LOCUS       DAKSQE010000001        77816 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.001, whole genome shotgun sequence.
ACCESSION   DAKSQE010000001 DAKSQE010000000
VERSION     DAKSQE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 77816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77816
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.001"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(181..738)
                     /locus_tag="O0V33_000001"
     CDS             complement(181..738)
                     /locus_tag="O0V33_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCX8974705.1"
                     /translation="MINFDKTALVLIDLQQGILKMDYAPHTAENVVQNANKLIEAFRK
                     NNGFIAFVRVNFYDGKDALQPNAMISLPPKEGDDYSHFHYLLDKRDDDFVIDKRQFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHVGVDTTARDAYQLNYDQYFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDLLNILN"
     gene            complement(805..2445)
                     /locus_tag="O0V33_000002"
     CDS             complement(805..2445)
                     /locus_tag="O0V33_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HCX8974706.1"
                     /translation="MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEQAGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENAATEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTAAKDTDASTYIELLVSKLHQSKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLSSLLQQLTDISYTNN
                     ASFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     SNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQEVF
                     NTINGHPDVMHFVEVKMSVEDAPKKLIDIAKAFSQQNK"
     gene            2637..2738
                     /locus_tag="O0V33_000003"
     CDS             2637..2738
                     /locus_tag="O0V33_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8974707.1"
                     /translation="MMLQNTIAMKKQARHLMKVYAPVYSKHYLASNN"
     gene            complement(2717..4762)
                     /locus_tag="O0V33_000004"
     CDS             complement(2717..4762)
                     /locus_tag="O0V33_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCX8974708.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGEALQHY
                     LPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQTGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
     gene            5347..6402
                     /locus_tag="O0V33_000005"
     CDS             5347..6402
                     /locus_tag="O0V33_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCX8974709.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKHEQVTYLIDANPSILTPSFYEHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSGLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     IRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDSVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            6402..7298
                     /gene="murQ"
                     /locus_tag="O0V33_000006"
     CDS             6402..7298
                     /gene="murQ"
                     /locus_tag="O0V33_000006"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCX8974710.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            7310..8764
                     /locus_tag="O0V33_000007"
     CDS             7310..8764
                     /locus_tag="O0V33_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCX8974711.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHNHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            8767..9641
                     /locus_tag="O0V33_000008"
                     /pseudo
     CDS             8767..9641
                     /locus_tag="O0V33_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            complement(9836..10117)
                     /locus_tag="O0V33_000009"
     CDS             complement(9836..10117)
                     /locus_tag="O0V33_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8974712.1"
                     /translation="MVLITYQIILFLIISLSYYLTLNHFMAVTVGNFTSIFGMFAAIL
                     FMYYYLLYKSPEYNQRKRFKHFIHITNLIIITFSTFVLVHLALKLFFNI"
     gene            10330..13119
                     /locus_tag="O0V33_000010"
     CDS             10330..13119
                     /locus_tag="O0V33_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCX8974713.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLSFLDTKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAHLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTVAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFI
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
     gene            13319..14182
                     /locus_tag="O0V33_000011"
     CDS             13319..14182
                     /locus_tag="O0V33_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HCX8974714.1"
                     /translation="MDNRNMINRVFSQKILHQIAIKNKSDVVDEAYDFYIQGPKNINV
                     IQKMKSLYNYLKKSYRNEYFYKNTMLNKLLLGLHSVNTTTALSEMPIGNSIADFILLN
                     GKGVVYEIKTELDKLDRLDNQINDYYEVFNYVVVITNDKHLNKVMARYKDTTVGILVL
                     TSRNTLSEVQKPKENNSLLNSKAMYNFLRKEERKRVIAQNHMDVPTYNDFTEYDVLFD
                     VFKEIPMTKLHNNMIFELKKRGNMKEYKDEFLAAPTEIKFLLYFAKMTKKDKNKLYHF
                     LKDTNNPPHFT"
     gene            complement(14387..15979)
                     /locus_tag="O0V33_000012"
     CDS             complement(14387..15979)
                     /locus_tag="O0V33_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8974715.1"
                     /translation="MSNLLEVNSLNVQFNYDETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLTNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVLFQHKRVSKSEAKSMTIDILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYTSPSGSLYRAVNDINLAIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKSLRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTIVSLLEKVGLDQSFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
     gene            15936..17414
                     /locus_tag="O0V33_000013"
     CDS             15936..17414
                     /locus_tag="O0V33_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8974716.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VISIITYLESFKSLYHKAFYKFVLSVLSLPLFLYAVIKFFFSAKRKNFYANNSEISEI
                     EQALHQKYKYLSQQKSSTQIHKEALKIFKTQSSNTNSKNIEQAHFSTYFENVLFHKFI
                     IIKVILALPMFILLTIYLQPLVRYIFERIVMAVIVIIGVIVSVFSILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNDVVTKAVGERIP
                     ITIIVAVLALIVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            17420..18583
                     /locus_tag="O0V33_000014"
     CDS             17420..18583
                     /locus_tag="O0V33_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8974717.1"
                     /translation="MVKLTTKIASLKLFASYAIATYILVILTSALNLFKGYVADTFYI
                     AETLLIVLTIILIIILTTEQTWKHHDLWRRIVEVLLLLMTLTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVVFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVTP
                     DVAEWGNILRTGSNYLETHSNLAIVPGICIMFVVLAFNFIGDAVRDALDPRIH"
     gene            18600..20375
                     /locus_tag="O0V33_000015"
     CDS             18600..20375
                     /locus_tag="O0V33_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCX8974718.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSRDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDNSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGPAKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            20413..22419
                     /gene="ggt"
                     /locus_tag="O0V33_000016"
     CDS             20413..22419
                     /gene="ggt"
                     /locus_tag="O0V33_000016"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCX8974719.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVVEPFASGIGGGGYLLYYEQSTGSITAFDARETAPAHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHSANWATEKYS
                     RQQHARLTKYHETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTITLEDLANYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLQSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDDYLKARSKL
                     IDSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVTGGINEIAPYKRPLSNMAPTIVMHHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRIYSSHPNRIEWEPQFSQSTILALIARGHAME
                     HKPDAYIGDVHGLHVDLNTRDASGGADDTREGTVMGGEVLSIRKQPLPYRQMYGSNVY
                     RVYFNDVQLPLLADQVRWMHDKYWVDESVVRIIFSEVSAHIEDLRSYENAGENYIDIT
                     WLARKKGYQVTLKDDGLYLTDDTYTSVKRNTNAYYRYDRDSITR"
     gene            complement(22956..23582)
                     /locus_tag="O0V33_000017"
     CDS             complement(22956..23582)
                     /locus_tag="O0V33_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCX8974720.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPEDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVLVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATTF"
     gene            23791..24369
                     /locus_tag="O0V33_000018"
     CDS             23791..24369
                     /locus_tag="O0V33_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCX8974721.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKLEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGKQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIDQLPDGERSLYDL"
     gene            24752..25849
                     /gene="ugpC"
                     /locus_tag="O0V33_000019"
     CDS             24752..25849
                     /gene="ugpC"
                     /locus_tag="O0V33_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCX8974722.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMANDKITLAFDMNKCHFFDEKTGNRIV"
     gene            25862..27133
                     /locus_tag="O0V33_000020"
     CDS             25862..27133
                     /locus_tag="O0V33_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCX8974723.1"
                     /translation="MSKILKYITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            27136..28404
                     /locus_tag="O0V33_000021"
     CDS             27136..28404
                     /locus_tag="O0V33_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCX8974724.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYVINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     SKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            28406..29245
                     /locus_tag="O0V33_000022"
     CDS             28406..29245
                     /locus_tag="O0V33_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCX8974725.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            29422..30498
                     /locus_tag="O0V33_000023"
     CDS             29422..30498
                     /locus_tag="O0V33_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCX8974726.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAACA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRPDSQFLYKSAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHDAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            30523..31563
                     /locus_tag="O0V33_000024"
     CDS             30523..31563
                     /locus_tag="O0V33_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCX8974727.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPHAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNCKLLTVAYHYRHTDVAITAKKAIESGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            31618..32586
                     /locus_tag="O0V33_000025"
     CDS             31618..32586
                     /locus_tag="O0V33_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCX8974728.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVAESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPTEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(32945..33439)
                     /locus_tag="O0V33_000026"
     CDS             complement(32945..33439)
                     /locus_tag="O0V33_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HCX8974729.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
     gene            33672..35051
                     /gene="uhpT"
                     /locus_tag="O0V33_000027"
     CDS             33672..35051
                     /gene="uhpT"
                     /locus_tag="O0V33_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCX8974730.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            complement(35410..36168)
                     /locus_tag="O0V33_000028"
     CDS             complement(35410..36168)
                     /locus_tag="O0V33_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCX8974731.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLAPCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(36161..37717)
                     /locus_tag="O0V33_000029"
     CDS             complement(36161..37717)
                     /locus_tag="O0V33_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCX8974732.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYIANTPRLSEIKKD
                     SRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            complement(37714..38682)
                     /locus_tag="O0V33_000030"
     CDS             complement(37714..38682)
                     /locus_tag="O0V33_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCX8974733.1"
                     /translation="MKSKIYILLLFLIFLSACTNTRHSESDKNVLTVYSPYQSNLIRP
                     ILNDFEKQEHVKIEIKHGSTQVLLSNLHNEDLSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIQGYQDLLQPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQDHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
     gene            39270..41519
                     /gene="pflB"
                     /locus_tag="O0V33_000031"
     CDS             39270..41519
                     /gene="pflB"
                     /locus_tag="O0V33_000031"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCX8974734.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            41542..42297
                     /gene="pflA"
                     /locus_tag="O0V33_000032"
     CDS             41542..42297
                     /gene="pflA"
                     /locus_tag="O0V33_000032"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCX8974735.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            42401..42568
                     /locus_tag="O0V33_000033"
     CDS             42401..42568
                     /locus_tag="O0V33_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8974736.1"
                     /translation="MRQVQQWLKEDAFVSSYADKECDALLKMGFQYHRMGYYLLLAYE
                     YESFQIFIDPE"
     gene            42619..44382
                     /locus_tag="O0V33_000034"
     CDS             42619..44382
                     /locus_tag="O0V33_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HCX8974737.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWMEIVSHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIISGATYLAICVDEEGDKFLVSSILFVVLKSSLFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKPKRKSRFFIISMVLAVTCFIGYNMYLLYN
                     NTINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDVVGLKMRQNGHEAKLVSLDEFIETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     SLDFFVIEDFSYSPRLVNQAHSENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(44546..44890)
                     /locus_tag="O0V33_000035"
     CDS             complement(44546..44890)
                     /locus_tag="O0V33_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HCX8974738.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSHLNLDAYQKRDILAAHYIAKSAIRTKNLDQMTKAKHRLESIY
                     DSISNPLHSQNN"
     gene            45080..46909
                     /gene="coa"
                     /locus_tag="O0V33_000036"
     CDS             45080..46909
                     /gene="coa"
                     /locus_tag="O0V33_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009557200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HCX8974739.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSKESRVNENSKY
                     DTPIPDWYLGSILNRLGDQIYYAKELTNKYEYGEKEYKQAIDKLMTRVLGEDHYLLEK
                     KKAQYEAYKKWFEKHKSENPHSSLKKIKFDDFDLYRLTKKEYNELHQSLKEAVDEFNS
                     EVKNIQSKQKDLLPYDEATENRVTNGIYDFVCEIDTLYAAYFNHSQYGHNAKELRAKL
                     DIILGDAKDPVRITNERIRKEMMDDLNSIIDDFFMDTNMNRPLNITKFNPNIHDYTNK
                     PENRDNFDKLVKETREAIANADESWKTRTVKNYGESETKSPVVKEEKKVEEPQLPKVG
                     NQQEDKITVGTTEEAPLPIAQPLVKIPQGTIQGEIVKGPEYLTMENKTLQGEIVQGPD
                     FPTMEQNRPSLSDNYTQPTTPNPILKGIEGNSTKLEIKPQGTESTLKGTQGESSDIEV
                     KPQATETTEASHYPARPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETN
                     AYNVTTNQDGTVSYGARPTQNKPSETNAYNVTTHANGQVSYGARPTQNKPSETNAYNV
                     TTHANGQVSYGARPTQNKPSKTNAYNVTTHADGTATYGPRVTK"
     gene            complement(47091..47198)
                     /locus_tag="O0V33_000037"
     CDS             complement(47091..47198)
                     /locus_tag="O0V33_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8974740.1"
                     /translation="MCVRTRLVSSSSVRLSKAIIIAVIVVCHIDVRGLF"
     gene            complement(47496..48680)
                     /locus_tag="O0V33_000038"
     CDS             complement(47496..48680)
                     /locus_tag="O0V33_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCX8974741.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALRTGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIQVNSVEYTNVGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GYKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            complement(48710..50971)
                     /locus_tag="O0V33_000039"
     CDS             complement(48710..50971)
                     /locus_tag="O0V33_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HCX8974742.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     LVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRTEQHKLSIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLYENGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDVIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDELGDLPQWISDLDGGFYKQDETIEYATPVSHFVKDELWDKGDAKLSVTHNDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENEHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRHGHFISDHDYHIALNIATILSGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            complement(51158..52369)
                     /locus_tag="O0V33_000040"
     CDS             complement(51158..52369)
                     /locus_tag="O0V33_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HCX8974743.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            complement(52481..53986)
                     /locus_tag="O0V33_000041"
     CDS             complement(52481..53986)
                     /locus_tag="O0V33_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HCX8974744.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFKAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDDDGDIFIIDR
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILDQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            complement(54012..55589)
                     /locus_tag="O0V33_000042"
     CDS             complement(54012..55589)
                     /locus_tag="O0V33_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCX8974745.1"
                     /translation="MEVTCLKQITWHDLQHIIKDDDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPNDLTFILANDIHSLGVAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     IDDETYLHYTFPSVDVALLRGTYADQQGNIYLTQESYLSECYHVALNAKANHGKVIVQ
                     VKDLVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQSLEQEVYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGELKQSIHKV"
     gene            55948..56619
                     /locus_tag="O0V33_000043"
     CDS             55948..56619
                     /locus_tag="O0V33_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCX8974746.1"
                     /translation="MRVNVLQHTPNEGVGYIGEWAKERGHEVYIYHPYMNDGVLPLAE
                     ETDMLVILGGPMNPDDEMPWIQSERQLILVLMAQNKPIYGACFGAQQISKALGYAVLK
                     SPVKEVGWDDVYLESEIIPNIPSRLKALHWHEDMFQIPQQAELLFSGNHIRNQGFIID
                     HHVIGLQFHFEPGPDDVREIVLNDAQYINHSILEQSAEDILAFEVPKQNRQAIFRILD
                     FITQA"
     gene            56843..57985
                     /locus_tag="O0V33_000044"
     CDS             56843..57985
                     /locus_tag="O0V33_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HCX8974747.1"
                     /translation="MSSASTQSTKTSDIHNESIDKQMEAKAHETAQNTDLKNEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMFSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVGLHGIWDTSLTI
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
     gene            complement(58296..59771)
                     /locus_tag="O0V33_000045"
     CDS             complement(58296..59771)
                     /locus_tag="O0V33_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCX8974748.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKVLVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
                     VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTSSAYPELVSELANPFMAIYDTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSLFNDKIPYIKEPKLTKQNIEQAKTLLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEDYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNNNVDDLINQLNHTVDVKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER"
     gene            complement(59969..60325)
                     /locus_tag="O0V33_000046"
     CDS             complement(59969..60325)
                     /locus_tag="O0V33_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCX8974749.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLYGAFKEKYEKELRDNETQKEAFEKLKAIVNQHHHVLLLYSAK
                     DTKHNQAVVLQQLLNT"
     gene            complement(60482..60655)
                     /locus_tag="O0V33_000047"
     CDS             complement(60482..60655)
                     /locus_tag="O0V33_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8974750.1"
                     /translation="MKLNQRYVKVFALYFASIVTANIVVKNNNLIKTLIQTLAGYTVF
                     AVGLKYLTKRKNK"
     gene            complement(60680..61825)
                     /locus_tag="O0V33_000048"
     CDS             complement(60680..61825)
                     /locus_tag="O0V33_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HCX8974751.1"
                     /translation="MLTEQEKDIIKQTVLLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGENL
                     LKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETDAYDFSQYVPGQYITVDVSSDKLPYRAKRHYSIVSGDKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIANHYDNAKLYTHLKGEQGYIGAEELKA
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVVV"
     gene            61980..62087
                     /locus_tag="O0V33_000049"
     CDS             61980..62087
                     /locus_tag="O0V33_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8974752.1"
                     /translation="MSVCTPTIARIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            62398..63351
                     /locus_tag="O0V33_000050"
     CDS             62398..63351
                     /locus_tag="O0V33_000050"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCX8974753.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDAEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(63672..65201)
                     /locus_tag="O0V33_000051"
     CDS             complement(63672..65201)
                     /locus_tag="O0V33_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCX8974754.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEI
                     VDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            65565..66500
                     /locus_tag="O0V33_000052"
     CDS             65565..66500
                     /locus_tag="O0V33_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCX8974755.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIVVAKGADRPLIKLVAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADEHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE"
     gene            66610..66713
                     /locus_tag="O0V33_000053"
                     /pseudo
     CDS             66610..66713
                     /locus_tag="O0V33_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            66838..68934
                     /locus_tag="O0V33_000054"
                     /pseudo
     CDS             66838..68934
                     /locus_tag="O0V33_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
     gene            68919..69386
                     /locus_tag="O0V33_000055"
     CDS             68919..69386
                     /locus_tag="O0V33_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCX8974756.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLKYN"
     gene            69409..69687
                     /locus_tag="O0V33_000056"
     CDS             69409..69687
                     /locus_tag="O0V33_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCX8974757.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            69914..71173
                     /locus_tag="O0V33_000057"
     CDS             69914..71173
                     /locus_tag="O0V33_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCX8974758.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQVICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQRVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            71191..72246
                     /locus_tag="O0V33_000058"
     CDS             71191..72246
                     /locus_tag="O0V33_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCX8974759.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCESKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDILRKKGFYSQIGIFKDAEITFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKALLKPLDLDENEGEN"
     gene            72248..72394
                     /locus_tag="O0V33_000059"
     CDS             72248..72394
                     /locus_tag="O0V33_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8974760.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            72418..73461
                     /locus_tag="O0V33_000060"
     CDS             72418..73461
                     /locus_tag="O0V33_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCX8974761.1"
                     /translation="MKALKLYGVEDLRYEDNEKPVIESANDVIVKVRATGICGSDTSR
                     YKKMGPYIQGMPFGHEFSGVVDAIGSDVTHVNVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIATSLGAHQTINSKEENLE
                     KFIENHYANQIDIAIESSGAKVTIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSNFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
     gene            73988..74704
                     /locus_tag="O0V33_000061"
     CDS             73988..74704
                     /locus_tag="O0V33_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCX8974762.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNTQGIN
                     DDDVIVTHDAVRPFLTQRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSAQKEILSDACKIIVESGHAVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
     gene            74697..75722
                     /locus_tag="O0V33_000062"
     CDS             74697..75722
                     /locus_tag="O0V33_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCX8974763.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEKHDVIAENY
                     LASSYFRSSGYDGFMQDYVVMAHDRIVPLPNDIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKTSFGIWGDGNLGYITAILLRKLYPEAKIYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKIDHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            75744..77438
                     /locus_tag="O0V33_000063"
     CDS             75744..77438
                     /locus_tag="O0V33_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCX8974764.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFLCRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKANYVLANEAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIDITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKANITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMENELPLIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDYREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     YESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
BASE COUNT        26041 a        12342 c        14620 g        24813 t
ORIGIN      
        1 tactaaattt tttgaaaatt ccggaataaa tactgttttt tatgtagtaa gggcataggt
       61 taagaacggt ttaacagata tagaatatgt ttatatagtg gaggttagac aacccgacgt
      121 attaatacat tgtgaaataa agataccccc ttcaatataa tcgaaggggg aagtgaatgt
      181 ttagttcaat atatttagca agtcgtttgt ggttatggtt tgtcccatca atgggaatac
      241 attatctatt gggaattgat gtagcgtttc gttttgtgca ctcatcatat ctgtaacaaa
      301 atactgatcg tagtttaatt gataggcgtc tcgtgctgtc gtatctacac caacatgtgt
      361 tgctacaccg ccaagaacaa ttgtatcaat tcctcgacgt ctcaactgta agtctaaatc
      421 cgttcctaca aatgcactaa attgtcgttt atctatgaca aaatcgtcat ctcttttgtc
      481 tagtaaataa tggaaatggc tgtagtcatc gccttctttt ggtggtaatg agatcattgc
      541 atttggttgc aatgcatctt taccatcata gaaattcacg cgaacaaagg cgataaagcc
      601 attgtttttt ctaaaagctt caattaattt gttagcattt tgaacaacat tttcagctgt
      661 atgtggcgca taatccattt taagaatacc ttgttgcagg tcgattaaca ctaaagcggt
      721 tttatcaaaa ttaatcatct tatatcagag tccttttcat ttaataatat attcgcttaa
      781 acttataccc tgaatacgat gaaattattt attttgttgt gaaaaagctt tagcaatatc
      841 gatcagtttc ttcggtgcat cttcgacaga cattttgact tcgacaaaat gcatcacatc
      901 gggatggcca ttaattgtat taaatacctc ttgtaaatct tttgatgatt caacgtcatg
      961 aatttcaaca tttttaccac caaatacagc tggtaaagct ttataatccc acatgtgaat
     1021 ttcattataa ggttcataca tgccgtgaat aagtcgttct accgtatagc cgtcattatt
     1081 aatcacaaat aataccggtt taatatgctg tctaatcata gttgaaatag cttgaacagt
     1141 tagttgcaat gagccatcgc caattaataa taagttacga cgatctttgt ctgctaattg
     1201 tgaacctaat gttgcaggta atgtatagcc gatagaaccc cataacggtt gccctataaa
     1261 agtattgctt ttgtataatg ctaaatcata agcaccaaaa aatgatgtac cttgatcagc
     1321 aataatgaca tcatttggtt ttaagaaatt ttgcatcatt ttaaaataag tttgttgtgt
     1381 taatggttct gtgccaacag tataatcggg tgatgttgga cgatgatatg caggaaacga
     1441 tgcgttattc gtatatgata tatcggtcaa ttgttgtagt aatgatgata gagatatttc
     1501 atcatttgta acatcatcaa ttttgatatt gtgatgattt aacataacga catcatcgat
     1561 attgaattgg tatgaaaatc ctgctgttgc tgaatctgtt aatttggctc caatatttaa
     1621 aattaaatcg ctgttgtcca cataatctcg tattttatct tcggcaattt tcccatcgta
     1681 aatacccata taatatggat tttcctcatt aaaagcacct tttcctaatg aaagttgtgc
     1741 tactggtatc tgtgtttgat ttacaaaatc ttctaattct tgatggaggt gaaaactgtt
     1801 aatttcatgt ccagtaatga tgataggctg cttcgattga tgcaatttag atactaataa
     1861 ctctatatat gttgatgcat cagtatcttt ggctgccgtt acttcgaatg gtgtcggtat
     1921 ctcaatttca gagattgcga catcgattgg taaatgtaaa tgaactgggc gtctttcggc
     1981 gattgctgta ttaattaagc gtggtatttc agttgctgca ttttcaggtg tgatataacc
     2041 ttgtgcaacg gttatatgtg caaacatttt tcggtagtcg tcaaatgtac cttcaccaag
     2101 tgagtgatgt acatatttac cggcttgttc aacagcacgt gtcggcgcac ctgtaatcgc
     2161 aatgacaggt atgcgttcag catacgaacc tgcgataccg ttgacggcac ttaattcgcc
     2221 aacaccaaat gtagtaacta atgcagcgag tccattaaga cgggcataac cgtccgctgc
     2281 gtaacttgcg tttaattcat ttgtatttcc tacccaatct acattgggat tactgataat
     2341 atcgtctaga aaagcgagat taaaatcacc aggaacacca aaaattttat cgacgcctgc
     2401 tcgatgaata gcgtcaatta agtaagctcc aatgcgttgt ttcataaaaa aaccaccttt
     2461 tctctatatt tacacttaaa atgatagcct cgaatatagt gtaaggtcaa ggaagttgac
     2521 tttgttgtga aaacgctaac aatagtgagc aattaatatt ttttaggaaa tgagtatgaa
     2581 gggggattta attgatgatt ttaaaattca aaatttaagg ggagagcgaa ctatcaatga
     2641 tgctgcaaaa cacaatcgca atgaaaaaac aggcgcgtca ccttatgaag gtatacgcac
     2701 ctgtttatag taagcattat ttagcttcaa ataattgatc gccaaatgaa atgttgccat
     2761 gttcaccttg tttaaaatca aggtttgtaa tgtttccttg tgtaacgata ataggcgtaa
     2821 tatcactctt tgcatgattg cggatgtagt ctaaatcaaa gttgattaat aaatcacctt
     2881 gtttaacttc ttgaccttcc tcaacatgta aagtaaagcc ttctccgttt aatttaacag
     2941 tgtctaaacc gatgtggatt aatagttcta aaccactatc tgatacaaga ccaattgcat
     3001 gttttgttgg gaaaatcatt tgtactttac cgttgaatgg tgcacgaact tcaccttgtg
     3061 aaggtttaat agcgataccg tcacccatca ttttttcgct gaacacttga tcaggcactt
     3121 ctgataatgg tgttacttca ccagttaatg gtgcatgcac gatatggctc aattcgcttg
     3181 ttgcagattt atcttctgca acaacaacgg tttcgtcttt atcgtcttcc atagtagtag
     3241 ggttttctac tacttgacca ttcataatct gttgcatttc atgtttgatt tggtcagatt
     3301 ttggaccaaa aattgcttgc atattattgc cgacttctaa tacaccagat gcgcctaaat
     3361 ctttcaaacc aggaacatca actttagatt tgtcgttaac ttcaacacgt agacgtgtga
     3421 tacaagcgtc taaatgttta atgtttgctt tgccacccat agcttctaat actgcatatg
     3481 gtaattcagt tgctgaagca gtagccgctt gtgattgttt atcttcacga cctggtgttt
     3541 tgtattttaa ttttacaatt aggaatcgga atacgaagta gtaaatgact gcgtatacaa
     3601 gacctacagg aatgactaac caccattgtg tcttattagg tagtataccg agtaagaagt
     3661 agtcgatgaa accacctgag aatgtataac ctagatgaag atctaataag tacaatgtta
     3721 agaatgataa accatcaagt actgcgtgaa taaagaataa taatggtgct acgaataaga
     3781 atgagaattc taaaggttct gtaataccag ttaagaatga tgttaaagca gcagaaccca
     3841 ttaaaccggc tactactttc ttgttttcag gtttagctgt gtgataaatt gctaaagctg
     3901 ctgcaggtaa accgaacatc ataacaggga attcaccttg catgaattta ccagctgtta
     3961 aatgtgcacc ttcacgaatt tgttcgataa agatacgttg gtcaccgtga ataatttcac
     4021 ctgctgcatt tttccatgaa ccaaactcga accagaacgg tgcgtggaaa atgtgatgta
     4081 gaccgaatgg aattaataaa cgcttgatga aaccaaataa gaatacggca acaccagtat
     4141 ttgagtctaa taatcctgta ctgaatgcat ttaatcctgt ttggattgtt ggccagatta
     4201 atgccattgg gaatgctaaa ataaatgatg ttgtagccat cataataggt acgaaacgct
     4261 taccagcgaa gaaacctaaa taagatggta agttaatgtt atagaacttg ttataacacc
     4321 aagctgccag ggccccgatt ataataccgc cgaacacacc tgtttgtaat gttgggatac
     4381 ctaaaatgct agcgtaacca ctcgctggat caccaatgtt cttaggtgtt acttgtaaaa
     4441 agtcacccat tgttttgttc atgattatgt aaccgacgaa tgctgcgata gctgctacac
     4501 catcaccgcc agctaatccg attgcgacac ctaatgcgaa aatcataggc aagttatcaa
     4561 aaatgatacc accagcacct gtcattaatt tagcgacagt ttgtacgcca ccattttgta
     4621 taaaaggcaa gtagtgttgt aatgcttcgc cttgcatagc tgtaccgata gctaataaca
     4681 gacccgctgc tggtaaaatc gcaacaggta acattagtgc cttaccaata cgttgcaatt
     4741 gaccgaaaag tttcttcctc acttgtccaa cctccaaagt tgtatttatt atttaagatt
     4801 caataaaaaa gacacgagca aatagactgt gttggtaacc atcacagctt aatttaactc
     4861 atgcctaatc ttacttagta acacgttggt gtatgtaatt aatgtaaaag aagcaattga
     4921 ctaagtagta tataaaacgg atacgttagt ttatctagct taccatcaca tcttattgaa
     4981 tcttatttgt ttgcgactcc tattttagca tggtgaagta tgcgttttca atacaattat
     5041 taaattcaat ttcaaatctc ttaattgttc acagattatt attaaaacat aatataagat
     5101 aatcctgaat aaaatgtata aaagttgttg taacaattca gcaatagcaa ctgttttcga
     5161 gcgttaaatg ataagaaaaa tatgtcgttt aaaaaaatta acttgtgaca gaaatatgta
     5221 agattattac aatgaacagg ctttatctga ttaatattgt aatgtgtttt ctcttatagt
     5281 aaaatgtaag cgattacaca actcaactat attttcctaa taattatatt gttaaggagg
     5341 gctactttga caggcttttc agtgtattta ggacaacctt tagatgaagc gtatattaag
     5401 cgaatgatta aacaaggtta ccaaatgatt tttacatctg tacaaatacc agaagaagat
     5461 gacgagacaa aatatcatta tttcacaaaa ctactcaatt tattaaaaca tgaacaagtg
     5521 acttacctca tagatgctaa tccatctata ttaacaccat ctttttatga gcatcttcga
     5581 caatatgatg cacaatttat gattcgtatc gatcatagta catcaattga ggcaatcgaa
     5641 gcgataatgg cacagggttt aaagtgctgt ttgaatgcaa gtattatttc ccgggaattg
     5701 ttaacaagct tacatcaaca attgaatgat tttacattac tttcattttg tcataactat
     5761 tatccaagac cagatacggg attatctgtt gacttggtca ataagaaaaa tgaactcatt
     5821 tatcaattta atccaaaggc acaaatatat ggttttattg tagggagtgg tttgcgaggt
     5881 cctttgcata aaggcttgcc aacaattgaa gcaacgagac atagtcatcc tgtcgttgca
     5941 gctaaattat tacaagaaac tggtgtatct gaagtgttag ttggagactc attgattgaa
     6001 ataaggcagg caaaacaact tatagatttt tgtaagcata ggcatttcac gttatgtatt
     6061 gaagaagtgt ttgatacgac agtgacttac cttttcgata tgtgtcataa agtacgtccg
     6121 gataatccgg agaatgtcat tcgttcggaa acgtcaagac aaatatgtcc gcattcgatt
     6181 caaccacagt ttacgacgca acgacgcatt ggttcagtaa ccgttgataa tttgaataac
     6241 ggacgttatc aaggtgaaat gcaaattgtg agacaaacgc ttagtgcaca tgacagtgtg
     6301 aatgttgttg cacaaattat taaagaagac ttaccactgt taagttgtat cgagccgaat
     6361 gatacatttg attttcaaaa aactagggag tgtaagaagt gatggaaaat agtacgaccg
     6421 aagcgcgtaa tgaagcgacg atgcatcttg atgaaatgac tgtggaagag gcattaatta
     6481 cgatgaataa agaagatcag caagtcccgt tagcagttcg aaaggcaata ccacaattga
     6541 caaaagtaat taaaaaaaca attgcacagt ataaaaaggg tggacgattg atctatatcg
     6601 gtgcaggtac aagtggacga ttgggtgtct tagatgcagc ggagtgtgta cctacattca
     6661 atactgatcc tcatgaaatt ataggtatta ttgctggtgg acaacatgct atgacgatgg
     6721 ctgtagaagg tgcggaagat cacaaaaaat tagcggaaga agatttgaaa aatatagatt
     6781 taacatcaaa agatgtcgtt ataggaattg ccgcgagtgg caaaacgcca tatgttatag
     6841 gcggtttaac atttgctaat acaatcggtg ctacaacagt atctatttca tgcaatgaac
     6901 atgcagttat aagtgaaatt gcgcagtatc cagtagaagt taaagttggt ccagaagtat
     6961 taactggttc aacgcgttta aagtctggta cagcacaaaa attaatttta aatatgattt
     7021 caaccatcac aatggttggt gtcggaaaag tttacgataa cctcatgatt gatgttaaag
     7081 caaccaatca aaaactgatc gaccgttcag tgcgtattat tcaagaaata tgtgctatca
     7141 catatgatga agcaatggcg ttatatcagg tatctgagca tgatgtgaaa gttgcgacag
     7201 ttatgggtat gtgtggcatt tctaaggaag aagcaacaag acggttatta aacaatggtg
     7261 acattgttaa acgagcaatc agagatagac aaccttagga gggatttaaa tgaccaaaga
     7321 acaacaactt gcagaacgaa ttattgctgc agtaggtggt atggataata tagatagtgt
     7381 catgaactgt atgacacgtg tgcgtattaa agtattagat gaaaataaag tagatgacca
     7441 agaactaagg cacattgatg gtgtcatggg tgttatacac gatgaacgca ttcaagttgt
     7501 agttggacct ggtacagtca ataaagtggc taatcatatg gctgagttaa gtggtgttaa
     7561 actaggtgac ccaataccac acaatcacaa tgatagtgaa aaaatggact ataaatcata
     7621 tgcagctgat aaagcaaagg caaataaaga agcacataaa gcaaaacaaa agaatggtaa
     7681 gttgaataaa gtattgaaat caattgccaa tatctttata ccgttgattc ctgcatttat
     7741 tggagctgga ttaattggtg gtattgcagc agtactgagt aacttaatgg tggcaggcta
     7801 tatttcaggt gcttggatta cgcaacttat aacagtattt aatgtcatta aagacggtat
     7861 gttagcatac ttagctattt tcactggtat taatgcggct aaagaatttg gtgcgacacc
     7921 aggacttggt ggcgtgattg gtggtacaac gttattaacg ggtattgctg gtaaaaatat
     7981 tttaatgaat gttttcactg gagaaccatt gcaacctgga caaggtggaa ttattggtgt
     8041 tatttttgcc gtttggattt taagtattgt cgaaaagaga ttgcataaaa ttgtgccaaa
     8101 tgcgattgat attattgtaa cgccgactat tgcattgttg attgtaggac tattaactat
     8161 ctttatcttt atgccattag caggttttgt ttcagacagt ttagtttcag tagttaacgg
     8221 aattattagt attggtggcg tatttagtgg atttatcatt ggtgcaagct tcctaccgtt
     8281 agttatgtta gggcttcatc atatttttac gccaattcat atagaaatga ttaaccaatc
     8341 tggtgctact tacttattgc caattgcagc gatggctggt gctggacaag taggtgccgc
     8401 attagcactt tgggtaagat gtaaacgcaa cacaacatta cgtaatactt taaaaggtgc
     8461 attgccagtt ggtttcctag gtattggaga accattaatc tatggtgtga ctttgccatt
     8521 aggtcgacct ttcttaactg cttgtattgg cggtggtatt ggtggcgctg taataggtgg
     8581 aattggacat attggtgcca aagcaatagg cccaagtggt gtgtcactat taccattaat
     8641 ctcagataat atgtatttag gttatattgc aggattactt gctgcgtatg ctggtggatt
     8701 cgtttgtaca tatttatttg gaacgacaaa ggcgatgcga cagacagatt tgttgggtga
     8761 ttaatgatga caaatatttt atatcgcatt gataagcagt tgagtgattt tacgaagaca
     8821 gaaaagataa tcgctgatta cattttaaag aatccacata aaatcattga tatgactgtg
     8881 aatgatttag cagatgttac gaatgttagt acagcatcaa ttgttagatt tagtcggaaa
     8941 atgacacatc aaggttttca agagctaaag attgcgatat ctcgatactt acccgaagat
     9001 attgcaacca atccacattt agaattgatt gaaaatgaat ctgtagaaac tttgaaaaat
     9061 aaaatgattg ctagagcaac gaatacgatg cgatttgtag ctactaatat tatggatgcg
     9121 caaattgatg caatttgtga tgtgttgaaa aatgcaggac aatattttta tttggatttg
     9181 gcgcatcgag tttgactatt ggtgatcttt ttcaaaagtt atctcgtatt ggcttaaatg
     9241 tcaggttatt acatgaaacg catttacttg tgtcaacatt tgcgacgcat gatgatagag
     9301 attgcatgat ttttgtgacg aatcaaggta gtcatagtga attgcagtca attgcacagg
     9361 tggccacaca ttacagtatt cccatcataa ctatatctag tacagctaat aatccagtgg
     9421 ctcaaattgc agactatgca ttgatttatg gcagaactga tgaaaatgaa atgcgtatgg
     9481 cggctacaac gtcactattt gcacagttat tcacggtaga tatattgtac tatcgatttg
     9541 tagcattaaa ttatcatgcg attctagatt gtataaccca atcgaaaatg gcacttgata
     9601 attacaggaa gcatcttgcg acgatagatt ttaaacatta gatattgtgt gcgttctaat
     9661 ataaagttag ttgcaacgta aacaattgaa gtttttgatg tgatttagtt gcttgatgaa
     9721 aaattgttga tacaagtttg taaaactggc atagaagtta ttttgaaaat gataaaagtg
     9781 tgacaaatta aagcaaagtt caggtttgag ctttgctttt ttcatagtat agaaattaaa
     9841 tgttgaagaa taattttaat gctaaatgaa ctaatacgaa ggtgctaaaa gtaattatta
     9901 tcaaattagt gatatgaatg aaatgtttaa atcgtttgcg ttgattgtat tcgggacttt
     9961 tatagagtag gtagtagtac ataaagagta tggctgcgaa catgccgaat attgaagtga
    10021 agttacctac agtgactgcc atgaaatgat ttaaagttaa ataataactt agactgataa
    10081 ttaaaaataa aatgatttga taggtaatta acaccattca agtccctcca ttaatcgtag
    10141 gcaaaaaatt tatcaatgat ttgattattt gtattcaaat tttagtatac gacttacctc
    10201 aaacacaaat attaaaatat aagacaataa aattagaata aatttacgtt tgaagataaa
    10261 aaacaaacat ttttcattaa agtttatgat atatttagag caatagaaag tgtatggaag
    10321 gggatatgaa tggcatacca aagtgaatac gcattagaaa atgaaatgat gaatcaactt
    10381 gaacaattgg gttacgaaag agtaacgata cgtgataata agcaattgct tgataatttt
    10441 agaacgattt taaatgagcg tcatgcggac aaattagaag gcaatccctt aacagataaa
    10501 gaatttcaac gtctgttaac gatgattgat gggaaaagta ttttcgaaag tgcccgtatt
    10561 ttacgtgata aattaccact tagacgtgat gatgagtctg agatttattt gtcgttttta
    10621 gatacgaaaa gttggtgtaa aaataagttt caagtgacga atcaagtatc ggtcgaggat
    10681 acatataaag cacgttatga tgtaacgata ttaatcaacg gactacccct tgtccaagtt
    10741 gaattgaaac gtcgaggcat tgatattaat gaggcgttta accaagtaaa acgttaccgt
    10801 aaacaaaatt acacaggctt attccgctac atacaaatgt ttatcattag taatggtgtt
    10861 gaaacgcgat acttttctaa taatgatagc gaactattga agagtcacat gttttattgg
    10921 agtgataaac agaataaccg aatcaataca ttgcaatcgt ttgctgagtc atttatgaga
    10981 ccttgtcaat tagctaagat gatatcacgc tatatgatta ttaatgaaac agatagaata
    11041 ctgatggcaa tgcgtccgta tcaagtgtat gcggtagaag cacttattca acaagcgact
    11101 gagacaggga ataatggata tgtgtggcat acaacgggaa gtggtaagac gttgacttct
    11161 tttaaagcga gtcagatttt atcacaacaa gatgacatta agaaagttat ctttttggtt
    11221 gaccgtaaag acttggatag tcaaacagaa gaggaattta ataaatttgc taagggtgct
    11281 gtcgacaaaa catttaatac ctcgcaactg gtacgccaac taaatgataa aagtttgcca
    11341 cttattgtaa cgacgattca aaaaatggct aaagcgattc aaggtaatgc ccatttatta
    11401 gaacagtata aaacaaataa agttgtattt attattgatg agtgtcatcg cagtcaattt
    11461 ggtgacatgc atcgtctagt taaacaacat ttcaaaaatg cccaatattt tgggttcact
    11521 ggtacgccac gtttcccaga aaatagtagt caagatggta gaacaactgc agatattttt
    11581 ggtagatgtt tacatacgta tttaattaga gatgccattc atgatggtaa tgtacttggt
    11641 ttctcagttg actatattaa tacttttaaa aataaagctt taaaagcaga agataacagc
    11701 atggttgaag caattgatac ggaagaagta tggttagcgg ataaacgtgt ggaattagta
    11761 acacgacata tcatcaataa tcatgataaa tatacacgta atcgtcaata ttcaagtata
    11821 tttacagttc aaagtattca cgcgcttatt aaatattatg agacatttaa gcgacttaac
    11881 aaaaagttgg aacaaccatt aacggtagct ggtatattta cgtttaaacc taatgaagat
    11941 gatcgtgatg gtgaagtgcc atatcattca cgtgaaaaat tagagataat gattagtgat
    12001 tataataaaa agttcgagac gaatttttca acagacacaa ctaatgagta ttttaaccat
    12061 atttcaaaaa acgttaaaaa gggcgttaaa gatagtaaaa ttgatatctt aatcgttgtt
    12121 aatatgttct taactggttt tgatagtaaa gtactgaaca ctttatatgt tgataagaat
    12181 ttaatgtatc atgatttaat tcaagcgtat tcacgtacaa atagggttga aaaagaatca
    12241 aagccatttg gtaaaattgt aaactatcgt gacttgaaaa aagagacaga cgatgcactg
    12301 agagtattct cacaaacaaa tgatacggat acaattttaa tgcgtagtta tgaagagtat
    12361 aaaaaagaat ttattgacgc ttatcgtgag cttaaaatga ttgtgccgac accacacatg
    12421 gttgatgaca ttcaagatga agaagagcta aagcgctttg ttgaagctta tcgtttatta
    12481 gctaaaataa tattacgttt aaaagcattt gacgagtttg agtttacaat tgatgaaatt
    12541 ggaatggatg aacaagagaa tgaagactat aaaagtaaat atttagccgt gtacgatcaa
    12601 gtaaaaagag cgacggctga gaaaaataaa gtatccattt taaatgatat tgatttcgaa
    12661 atagaaatga tgcgtaatga tacgattaat gtgaattata ttatgaatat attgagacaa
    12721 attgatcttg aagacaaagc ggaacaacgt cgtaaccaag aacaaattag acgtatttta
    12781 gatcatgcag atgatccgac attgaggtta aaacgagatc taattagaga attcatcgac
    12841 aatgttgtac cttctttaaa taaggatgat gatatcgatc aagaatatgt taatttcgaa
    12901 agtattaaaa aagaagcgga gttcaaagga tttgctggag agagatctat cgatgaacaa
    12961 gccctaaaaa caatttcaaa tgactaccag tatagtggtg ttgtaaaccc acatcacctt
    13021 aaaaaaatga ttggtgattt gccattgaaa gaaaaacgta aagcaagaaa agccattgaa
    13081 tctttcgtgg cagaaacaac tgaaaaatac ggtgtgtaat gattcagccc cctcgctaga
    13141 ttagtgtagg gggcattatt atttcattag aaaaggctca tgtgtttggc aatcatcatt
    13201 tggtttcaat acaattttat catcgtattt gtatagaaat aatgtctata ggcatttaaa
    13261 atagtttata atattaacag gataagtatt aaatgtatgt tgaaggaggt gcgtagctat
    13321 ggataataga aatatgatta atcgtgtttt tagtcaaaag atattacatc aaattgcaat
    13381 caaaaataaa agtgatgttg ttgatgaggc atatgatttt tatatacaag ggcctaaaaa
    13441 tatcaatgta atacagaaaa tgaagtcttt atataactat cttaaaaagt cttatcgtaa
    13501 cgaatatttt tataaaaaca caatgcttaa taaactcctt ttaggactac attctgttaa
    13561 tacaactact gcactttctg agatgcctat aggaaatagt attgctgatt ttatattgtt
    13621 aaatggtaaa ggcgttgtct acgagattaa aacagaatta gataagttgg atagattgga
    13681 taatcaaatt aatgattatt atgaagtatt taattatgta gtagtcatta caaatgacaa
    13741 acacttgaat aaagttatgg ctagatacaa agatacaaca gtcggaattt tagtattaac
    13801 atctagaaat acactgagtg aagttcaaaa accaaaagaa aacaatagtc tcttaaactc
    13861 aaaagcgatg tacaactttt tacgaaaaga agaaagaaaa agagttattg cacaaaatca
    13921 tatggatgtg ccaacttata atgatttcac agagtatgat gtgttatttg acgtgtttaa
    13981 agaaatacca atgacgaaac tgcataacaa tatgattttt gagttgaaaa aaagaggcaa
    14041 catgaaagaa tacaaagatg aatttttagc agctccgact gaaattaagt tcttgttata
    14101 tttcgcaaaa atgacaaaga aagataaaaa taaactatat cattttctta aggatacaaa
    14161 taatcccccg cacttcacat agtggcgggg gatttatcat gcgttaaaat gcccaaagta
    14221 tgggtgtgac ccttaaatca tggcaaagca tctaacactt gatatggtgc cgagccctaa
    14281 catagcacaa tatagttagt atgacaaagt catctaacat aacacatcag atgttgcccg
    14341 atttattcca gaccatacag tgataaagtt gcacaacgca tgacttttat ttagcaataa
    14401 ctgctacttc tgaaataagt tgctttgcat agtctgattg cggatgtttg ataatatctt
    14461 ctgtgttatt aagttcaacg atttcgccat ttttcataac tgcaactcga tcacatattt
    14521 cattgataac acccatgtca tgtgtgatga ataaataagt gatgccgaag tctaactgta
    14581 attgttttaa taactcgatg atatcttttt gaattgaaac gtctaaagct gatactgcct
    14641 cgtcgcaaac aatcacttta ggttctacag caagtgctct tgcgatactt acacgctggc
    14701 gttgcccacc agataattcg tgtggatagc gatataagaa actttgatct aggccaactt
    14761 tttctaacaa cgatacgata gttttaataa tgtcgtcatt atctttgact ttcccatgaa
    14821 tgattagtgg tcgtttaatc acatcaatga ctttaaatct tggattaata gatgcgaatg
    14881 gatcttggaa aatcatttgt atctcttgtc gtaaagattt caattcatca tctttaaata
    14941 aacttaatgg taattcgtta taccaaataa agccttctga cacttccttt agaccgacga
    15001 ccgtcttagc taatgtcgat ttccctgacc ctgattcacc gacaatgcct aacgtttcgc
    15061 cttttctaat agccaagtta atatcattaa ctgctcggta taggctgcca ctcggtgatg
    15121 tgtaatccac gctcacgcga tcgaatttta ataaaatatc attgtttaac ggtcttggcg
    15181 gacgcgtttg atgaatatca ggaatcgcat ctattaagcg tttcgtatag gtatgttgtg
    15241 gcgatttaaa aatactttca accgtgccac tttcaacgac acttccatct ttcattacaa
    15301 tcacatcgtc gcaaaattga tacacagcac ctaaatcgtg agtgataaaa ataatagacg
    15361 tttctgtgta ctcataaagg gacttcatta actgcagtaa ttgattttgt gtactggcat
    15421 ctaatgccgt tgttggttca tctgcgatta aaatttgtgg ctttaaaatc aatgccattg
    15481 ctatcatgac acgttgacgc ataccaccag aaagttcatg cggataagca tcaaattgtc
    15541 gagttgcatg ttttatacct actttttcta aaatgtctat tgtcatcgac tttgcttcag
    15601 atttagatac acgtttatgt tgaaatagta cttctgtaat ttgtttgcca atcgttaatc
    15661 ttggattcaa cgaagagagt ggatcttgaa aaatcattga aatatccttg cctcgaattt
    15721 gttgtaacgc tgaagttgat aaattagtta acgattgccc attaaaaata atttctcctg
    15781 ttaacgtgtg atccggataa tctggtagta gccctaaaat agatttagcg gtaatacttt
    15841 ttcctgatcc tgattcacca acaataccta ggatatgttt ttttcgtaat tcgaaagaga
    15901 cgttttttac cgcttgaact gtagtttcat cataattgaa ttgtacattc agactgttga
    15961 cttctaataa atttgacatg atgttgtgcc tccctattca caaatgtgta aatttattct
    16021 atacttttgt atatcatagt aatctactca agattatttt acaaggagtg attagcataa
    16081 ttacttattt agaatctttt aagtcgttgt atcacaaggc attttacaag tttgtattat
    16141 cggtattaag cttgccatta tttttatatg cagtgataaa gtttttcttt tctgctaaga
    16201 gaaaaaattt ttacgctaat aattctgaaa tttcagaaat cgaacaggcg ttacatcaaa
    16261 aatataaata tttatcgcag caaaagtcat ccacacaaat acataaagaa gcattaaaaa
    16321 tattcaagac acaaagttct aatacgaatt caaagaatat tgaacaagca catttttcaa
    16381 catactttga aaatgtatta tttcataagt tcatcattat caaagtgata ttggcgttac
    16441 cgatgttcat cttattgact atttatttac agccattagt tagatatatt tttgagcgaa
    16501 ttgtcatggc tgtgattgtc atcattggtg ttattgtcag tgtgtttagc attctgtatt
    16561 tttcaccgct tgatgcggct tatagcatac tgggacaaaa tgcaacaaag gcacagatac
    16621 atcaattcaa tgtattacat catcttaacg aaccttattt tattcaattg tgggatacca
    16681 tcaagggtgt ttttaccttt gacttaggta cgacttacaa agggaatgat gttgtgacta
    16741 aagcagttgg cgaaagaatt ccaattacaa taattgtcgc agtattagcg ctaattgtgg
    16801 cattaattat tgcaatacca attggtatta tcagtgcgat gaagcgaaat agttggcttg
    16861 atatcacgtt aatgataatt gcattaattg gtttgtctat tccaagtttc tggcaagggt
    16921 tattattcat tttagcgttc tcattgaaat tggatatttt gccaccatct tatatgccag
    16981 aacatccaat atcgttgatt ttacctgtac ttgtcattgg aacaagtatt gctgcttcta
    17041 tcacgcgtat gacaaggtct tctgtacttg aagtaatgcg cagcgattat gttttaactg
    17101 cttatgcaaa aggattatcg acgacacaag tggttattaa acatattttg aaaaatgcca
    17161 ttattccaat tgtaacgcta gttggtcttc tagtggcaga gttactaggc ggttcagcag
    17221 tgacggagca agtatttaac attaatggta tcggtcgtta tatcgtccaa aaacaactaa
    17281 tacctgatat cccagcagtc atgggtgggg tcgtatatat atcaattgta atatctttag
    17341 caaacttaat tattgatata ttttatgctt taatcgatcc aaaattacgt agtgaaatta
    17401 acgaaaggaa gtgaggcata tggtaaaact tacaacaaag atagcttcct taaaactatt
    17461 cgcaagttat gctattgcaa cttatatttt agttatatta acgagtgcat taaatctttt
    17521 taaaggttat gtggccgata cgttctatat tgctgaaaca ttgctaatcg ttttaaccat
    17581 cattttaatt atcatcttaa caacggaaca aacatggaag catcatgacc tatggcgacg
    17641 tatcgtcgaa gtgttgttat tgttgatgac attaacaggg aacgtattta cattattaat
    17701 gtttgtaagt attagacgtt accaacgtac atcgcaaata catagttata atggctggga
    17761 atcgtttata cgaaaaacta ctagacatcg tattgcgatt atcgggttac ttattttagt
    17821 ctatatgctg acattatcaa ttgtgtcaca atttacattt gatacgacat tggctactaa
    17881 aaatcagttc aacgcactgt tacatggacc gagtctagcc tatccgtttg gtactgatga
    17941 tttcggtaga gacttattta cacgcgtagt tgtcggaacg aagctgacat tttcaatttc
    18001 aattatttca gtagttattg cagttgtttt tggtgtgtta ctaggcacta tcgcaggtta
    18061 ttttaatcat attgataatt taataatgcg aattttagat gtagtgtttg caattccatc
    18121 attattgtta gcggtggcaa ttattgcatc gtttggagca agtattccaa atttaattat
    18181 tgctttaagt atcggtaata taccatcatt tgcacggaca atgcgtgcca gtgttttaga
    18241 aattaaacgc atggaatatg tagatgcagc acgtatcact ggtgaaaaca cttggaatat
    18301 catatggcgt tatattttgc cgaatgcgat tgcgcctatg attgtacgtt tttcattaaa
    18361 tataggtgtg gttgtattaa caacaagtag tttaagtttc ctaggacttg gtgttacacc
    18421 tgatgtagct gaatggggca acattttacg taccggtagt aactacttgg aaacgcacag
    18481 taatttagct attgtacctg gtatttgtat tatgttcgtc gttttagcat ttaattttat
    18541 aggtgatgca gtgcgtgatg cgctagatcc aagaattcat taaaaaggta gggatagatg
    18601 tgaagaaaat cattagtatc gcaattatag ttttagcgtt ggtattaagt ggttgtggtg
    18661 tccctacgaa atcagaagtg gctcaaaagt catcgaaagt tgaagtgaaa ggcgagcgac
    18721 caacgataca tttcctagga caagcaagtt atgaaaatga tatgaatatc gtcaaagatc
    18781 aattggaaaa tgcaggattt aacgtgaaga tgaatatcca accagattat ggtagctatc
    18841 gtacacaacg tcaagccggc aattatgata tccaaatcga tgactggatg acagtattcg
    18901 gtgacccgaa ctatgctatg acggcattat ttagttctac aggatcaaat agtttattga
    18961 aagataaaca tgtagaccag ttgttaaata aagcttctac tcaaaatgaa gcagatgtta
    19021 aacaaacata taagcaaatt gaagatgaag ttgtatttga taaagggtat atggcgcctt
    19081 tatatggatc aaaaaagaat ttagtatatg acaataaagt gttagataaa aatagtgttg
    19141 gattgccaaa ttcacgtgca ttaatatggc aacaatttga ttacaacaat agtagagaac
    19201 gagatacgcg gccacttgtg atgacacaac aagacggtga aattcctaca ttggatccaa
    19261 tacgttcaat tgcgccgtca gtatattcaa ttaatatgaa tatgtacaca aggttattat
    19321 tattagatga aaatgaccac ttaacaacga aaggttcgtt aagtcgtgat tatgctgtga
    19381 ataaggacaa taaagcgttt tatttcttat taagagatga tgattatttt gcgaaagtgg
    19441 ttaatggaca agcacgtaat actggagagc gtgtatcggc tgaagatgtt aagttttctt
    19501 tagatagagc acgtgataaa aagtctgtgc ctaacaataa tacttacaat atgcacaaac
    19561 atataaatga catcaagata ttaaaagatg aggacatcga tcagttgcgt aaagagaagg
    19621 acaaggacga taattccatc tatgataagt tgattaaagc gtataacgtc aaatcgttaa
    19681 cgacagatgg tcaaaaagta aataataaag acggtattta tcaaattgtt aaaattacga
    19741 cagatcaatc gatgcctcga gaggtaaatt acttaacaca ctcttcggca ggcattttat
    19801 ctaaaaaatt tgttaatcaa gtaaatcaag aatatccaaa aggatatggg gatagcagta
    19861 ccattcctgc aaattcagat ggaaaaaatg cgctttatgc aagtggcgca tacattatga
    19921 cacagaaaaa tgcatatcaa gcaacgtttc aacgtaatcc aggattcaac gaaacagaaa
    19981 aaggtagtta tggaccagct aaaattaaaa atattacatt gaagtttaat ggtgacccga
    20041 ataatgcatt gtcagaactt agaaatcatt caattgatat gttggcagat gtgaatcaaa
    20101 aacattttga tttaattaag tcggataaaa atttaagcat tattcgcaaa aatggacgta
    20161 agtcagtctt tttaatgcta aatattaaaa aaggtatatt taagacgcat ccaaacttga
    20221 gacaagcagt agttaatgcg atagatcagg atcaatttat taagttttat cgtggcgata
    20281 aatttaaaat tgcatcaccg attacaccac ttgtcgatac tggtaacgag caacgtcaag
    20341 atttagaaaa agtagaaaaa gccattaatc aataatgttt taaatattta acagaaagta
    20401 ggaggatata gtatggtcat taacttaaat gacaaacaga caaaaacatc taaagaaggg
    20461 ttaatttccg tatcacatcc tcttgcggct aaaattggta aggatgtatt agatcaaggt
    20521 ggcaacgcta tggatgccgt gattgcaatt caactggcat tgaatgtggt agaaccattt
    20581 gcatcaggta ttggtggtgg cgggtacttg ctatattatg agcaaagtac tggcagtata
    20641 actgcgtttg atgcgcgtga gacagcccct gcacatgtag acaaacaatt ttatctagat
    20701 gattcaggcg aatataaatc attttttgat atgactacac atggtaaaac tgtcgctgtg
    20761 ccagcaattc caaagctgtt tgattatatt cacaagcgtt atgctaaatt gtcattggaa
    20821 gatttaatta atcctgcaat tgaactagcc attgaaggtc attcagctaa ttgggctact
    20881 gaaaaatatt cgcgccagca acacgcgcga ttgacaaagt atcacgaaac ggcacaagta
    20941 tttacccatg aaaatcaata ttggcgtgaa ggtgattgga ttgtacaacc cgaattaggt
    21001 aagacatttc aaatattaag agaacaaggg tttaatgcat tttataaagg tgacattgcg
    21061 aaacaattag tcaatgttgt caaagcatgt ggtgggacaa tcactttaga ggatctagcc
    21121 aattatgata ttcagattaa agcgccaatc agtgcgacat ttaaagacta tgacatttat
    21181 tcaatgggac catctagttc tggcggtatc acggtaattc aaatattgaa gttattagaa
    21241 catgtcgatt tacaatctat gggtccaaga tctgttgatt acttgcatca tttgatacag
    21301 gcgatgcatt tagcatatag tgatcgcgcg caatacttgg cggatgataa ttttcatgag
    21361 gtgcctgtac agtcattaat tgatgacgat tatttaaaag cacgcagtaa gctcattgat
    21421 agcaataaag caaatattga tatagagcat ggtgttgtgt ctgattgcat tagtcataca
    21481 gatgttgaag aaaatcatac cgaaacaact catttttgtg tgattgataa ggaaggtaat
    21541 attgcttcat ttacgacatc aatcggtatg atttacggat cgggtatcac gattccaggc
    21601 tacggcgtgt tattgaatac cacaatggat ggttttgatg ttgtgacggg tgggatcaat
    21661 gaaattgcac cttataaacg accactaagt aacatggctc caacgattgt gatgcatcac
    21721 gggaagccaa tattaacagt aggtgcacct ggtgctataa gtatcattgc tagtgttgcg
    21781 caaacattaa tcaatgtatt agtgtttggc atggatattc agcaggctat agatgaacct
    21841 agaatttata gtagccatcc taatcgcatt gaatgggagc ctcaattttc acaatctaca
    21901 attttagcat tgattgcacg tggacatgca atggaacata aaccagatgc ctatattgga
    21961 gatgtacatg ggctacatgt tgacttgaat acacgtgacg catcgggagg tgctgatgat
    22021 acgagggaag gtacagtgat gggtggtgag gtattatcaa ttagaaaaca accattacca
    22081 tatcgtcaaa tgtatggtag taatgtatat cgtgtatatt tcaatgatgt gcagttacct
    22141 ttattggcag atcaagtgcg atggatgcat gacaaatatt gggttgatga aagtgtcgtt
    22201 agaatcatct tctctgaagt tagtgcacat attgaagatt taagaagtta tgaaaacgca
    22261 ggagaaaatt atatagatat tacctggtta gcacgaaaga aaggttatca agtaacattg
    22321 aaggatgacg gtttatactt aactgatgat acatatactt cagtgaaacg gaacacaaat
    22381 gcatactata gatatgatcg agatagtatc acaagatagt ttatacgctt gatatgaagt
    22441 ttgtagtgga taaggacttt tgttgaaaga aaggattcga tttcgaactt tagcgtgacg
    22501 cttgagtgta gtaatgttgg aaatgctata cgaaacgcta gtcacctttg taaatataca
    22561 acacctcaca tattcgtttt tgatgtacat cacaatagtt agtctaaaat ctaacgtgat
    22621 gtggtttggt acagttgaac gagttatgtg aggtgttttt ataatgtggg attttaatag
    22681 atgatttgga tgtttctata attacagcat aagtattaat gttcgctact aagtttaacc
    22741 agaagttttt atgtgtcttc atactttcat atctactctc aaaatatatc atcaacacta
    22801 tcatatgttt taaaaatatt tacatcataa ctaaagtgaa tgtcaaaatt taaatttgta
    22861 aaaacaatag atgtatataa aaagttgaaa aggattgtaa gaaaatcaaa aaaacactgt
    22921 atgacgtttg attagtcaca cagtgtagat aacaattaaa atgttgttgc caatttttca
    22981 gcattattaa tactagttgc tttaatttct tcagttttat gcggttcggc attgtgtcct
    23041 tcaataatga ttgtttcata tgatggcaca cctaagaatg tcataattgt tcttaaataa
    23101 cggtcaccca tttcaaaatc agcagcaggt ccttcagtat aatatccacc acgtgattga
    23161 atgtgtaata cttttttgtc agttagtaaa ccttgtggtc cttcagcaga atatttaaaa
    23221 gttttacctg caattgaaat agcatcaata tatgctttaa ctacaggtgg gaaagaaagg
    23281 ttccacatag gcgttacaag cacatattta tctgcactta aaaattcttc taaaatgtca
    23341 ctcaatcttg aaactttcat ttgttcatca tcagttaacg tttcgccatt actcattttc
    23401 ccccaaccag ttaatacatc tttgtcaata actggaatat aagtttcaaa taaatcaata
    23461 tgtttcactt catcttcagg atgttgttgt tgatatgttt cgataaatgc tttaccagcc
    23521 gccatagaat ttgataccag ttcattaaaa gggtgtgctg taatatataa tacttttgcc
    23581 atttgaaaat tctcctctgt ttctgttatt ttcttaagta taattattat actaggtata
    23641 aaatttaata tcaatcaaaa tattcaaatt accatcattt tcttcatcta tattttgcag
    23701 tactactaaa gtatgagtgc atttaattat gaaatagttg atttagaata tatacttaat
    23761 acccaaaata tatgaaggat ggatgccact atgacaaagc gaccaaaacg tattttggca
    23821 acaattatca tttttctttc actattattt acgattattt atatagatga catacaaaaa
    23881 tggtttaacc aatataccga taaattgaca caaaatcata aaggacaagg acactcaaaa
    23941 ttggaagact tttttagagg gagtcggatt actgagactt ttggtaaata tcaacattca
    24001 ccatttgatg gtaagcatta tggtattgat tttgcattgc caaaaggtac accaattaaa
    24061 gcgccgacga atggtaaagt aacacgtatc tttaataatg aattaggcgg caaggtatta
    24121 cagattgccg aagataatgg agaatatcac cagtggtatc tacacttaga caaatataat
    24181 gtcaaagtag gtgatcgagt caaagcaggt gatattattg catattcagg caatacaggt
    24241 aaacaaacga caggcgcaca tttacatttt caaagaatga agggtggcgt aggtaatgca
    24301 tatgcagaag atcctaaacc gtttatcgat cagttacctg atggggaacg tagcctatat
    24361 gatttgtagt tatagaagag tgcccgcagt ctaaaaaatc aagcaatcat tgtgtgagta
    24421 taatacttac ataatggttg cttttttcaa tgaaaatcga aatgctaagt catacttgtt
    24481 tgatttagat attatttaaa atgtaagaca aggttgttag cattggcagt gaaatttcgc
    24541 acgtaaaaaa tcattattga cacactaaaa atagttgtgt gctatatcaa ttttctgtat
    24601 aaaagtttaa ttctgacagt aatgtaaacg tttacaattt atgattgaca ttaataataa
    24661 gtgactatat gatttatgta agtatttgtg caaacgtttt cacaaagtgt attgcacaat
    24721 caaactgtaa acaaagtatg ggaggcataa catggcagaa ctaaagttag agcatattaa
    24781 aaagacgtat gataacaaca atactgtagt gaaagatttt aatctacata ttactgacaa
    24841 agaattcatt gtatttgttg gaccatcggg atgtggtaaa tcaacaacat tacgaatggt
    24901 tgctggacta gagtctatca catctggaga tttttatatt gatggggaac gcatgaacga
    24961 tgttgaacca aagaatagag atattgcgat ggtatttcaa aactatgcat tatatccaca
    25021 tatgactgtt tttgaaaata tggcatttgg gctaaagcta cgtaaagtga ataaaaaaga
    25081 gattgaacaa aaagttaatg aagcagctga aatattagga ttaactgagt atcttggtcg
    25141 taaaccaaaa gcgttatctg gtggacagcg tcaacgtgtt gctttgggca gagctattgt
    25201 tagggatgcg aaagtctttt taatggatga accattatcg aatcttgatg cgaagcttcg
    25261 agtacaaatg cgcacagaaa tattgaaatt acataagcga cttaatacta cgacaattta
    25321 tgttacgcat gatcaaactg aagcattgac gatggctagt cgaattgttg ttttgaaaga
    25381 tggcgacatt atgcaagtcg gcacacctag agaaatatat gatgccccta attgcatttt
    25441 tgtggcgcaa tttatcggct caccagcaat gaatatgttg aatgccacag ttgaaatgga
    25501 tggattgaag gtaggaacac accattttaa attacataat aaaaaatttg aaaagttaaa
    25561 agctgctggc tacttagata aggaaattat tttaggtatt cgagctgaag acattcatga
    25621 agaaccaata tttattcaaa cctctccaga gacacaattt gaatctgaag tagttgtatc
    25681 cgaactgtta ggttcagaaa ttatggtaca tagcacattc caaggaatgg aattgatttc
    25741 taaattagat tcaagaacac aagtgatggc gaacgacaag attacactag catttgatat
    25801 gaataagtgt cacttttttg atgaaaaaac aggaaatcgt atcgtctaag ggggagtatt
    25861 catgtctaaa attttaaaat atatcacgtt agccgtggta atgttattaa tcgtaactgc
    25921 atgtggccct aatcgttcga aagaagatat tgataaagca ttgaataaag ataattctaa
    25981 agacaagcct aaccaactta cgatgtgggt ggatggcgac aagcaaatgg cgttttataa
    26041 aaaaattacg gatcaatata ctaaaaaaac tggcataaaa gtaaagcttg taaatattgg
    26101 tcaaaatgat caactagaaa atatttctct agacgctcct gctggaaaag gtccagatat
    26161 ctttttctta gcacatgata atactggaag tgcctatcta caaggcttag ctgctgaaat
    26221 caaattatca aaagatgagt tgaaaggctt caataagcaa gcacttaaag cgatgaatta
    26281 tgacaataag caattagcat tgccagctat cgttgaaaca accgcacttt tttataataa
    26341 aaaattagtg aaaaatgcac cgcaaacgtt agaagaagtt gaagctaatg ctgccaaact
    26401 aactgatagt aaaaagaaac aatacggtat gctatttgat gctaaaaatt tctattttaa
    26461 ttatccgttt ttattcggca atgatgatta tattttcaag aaaaatggca gtgaatatga
    26521 tattcatcag ctaggactaa attcaaaaca tgtcgtcaag aatgctgaac gattacaaaa
    26581 atggtacgac aaagggtatc ttcctaaggc agcaacacat gatgtcatga ttggtctttt
    26641 taaagaagga aaagtaggac aatttgtcac tggaccatgg aacattaatg aatatcaaga
    26701 aacgtttggt aaagatttag gagtaacaac attgcctaca gatggtggca aacctatgaa
    26761 accatttctc ggtgtacgtg gttggtattt atctgaatat agtaaacata agtattgggc
    26821 taaagattta atgttgtata tcactagtaa agatacatta caaaaatata cagatgaaat
    26881 gagcgaaatt actggacgtg ttgacgtaaa atcatctaat ccaaatttaa aagtgtttga
    26941 aaagcaagca cgtcatgctg aaccgatgcc taatattcct gaaatgcgac aagtttggga
    27001 accgatgggc aatgcaagca tatttatctc aaatggtaag aatcctaaac aagcgttaga
    27061 tgaggcgacg aatgatataa cgcaaaatat taagattctt catccgtcac aaaatgataa
    27121 gaaaggagat tagttatgac gaaacgtaac cctaaattag cggcattatt atctgttata
    27181 cctggtttgg gacagtttta taataaaaga cccattaaag ggacgatatt ttttatcttt
    27241 ttcatcagtt ttatttctgt attttatagc tttttaaata ttggtttttg gggattgttc
    27301 acattaggga cagtacctaa gttagacgat tctcgtgtct tacttgcaca aggtattatt
    27361 tctatcttac tcgttgcttt tgcaatcatg ctatatgtca ttaatatttt agatgcatat
    27421 cgtaatgctg aacgatttaa tcgcaatgag gaaataaagg atccgaaggc acgtatggtt
    27481 gcaacatggg acaagacgtt cccatactta ctaatctcac caggtacatt cttattgata
    27541 tttgtagttg tatttccatt aatatttatg tttggagtag catttacaaa ctacaattta
    27601 tacaacgcgc ctccgagaca cacattagaa tgggttggtt tagataactt taaaacgtta
    27661 ttcacaattg gcgtttggcg taaaacattt ttcagtgtta ttacttggac attagtatgg
    27721 acgcttgttg caacgacact tcaaattgca ttagggctgt ttttggcaat tattgtaaat
    27781 caccctgtcg tcaaaggtaa gaaatttatc cgcactgtgt taatcctacc ttgggctgta
    27841 ccatcatttg tgacaatttt aatatttgta gcgttattta atgatgaatt tggtgcgata
    27901 aataatgata tattgcaacc tttattaggt gttgcaccag catggttaag tgatccattt
    27961 tggtcaaaag tggcattaat tggtattcaa gtatggcttg gtttcccatt cgtctttgca
    28021 ctgttcactg gtgtactgca aagtatttca tcagattggt acgaagcagc agatatggat
    28081 ggtgcgtcta gttggcaaaa gttcagaaac atcacattcc cgcatgtcat ttacgccaca
    28141 gcgccattgt taattatgca atatgcaggt aatttcaata attttaatct tatttatcta
    28201 tttaataaag gtggtccacc agtgtcaggg cagaatgctg gtagtacaga tatcttgata
    28261 tcttgggtgt ataatctgac atttgagttt aacaacttta acatgggtgc agttgtgtca
    28321 ttaattattg gatttattgt tgctattgtc gcatttattc aattcagacg tacaagtacg
    28381 tttaaagatg agggaggttt ataagatgac aaagaagaaa aacatattaa aagcaatcgg
    28441 tatttacagt tttatagcga tgatgtttgt catcatttta tatccactac tgtggacatt
    28501 tggcatttcc cttaatccag gtacgaactt gtatggtgcc aaaatgatac cagacaatgc
    28561 aacatttaaa aattatgcat tcttactatt cgatgacagt agtcaatacc tgacttggta
    28621 taaaaatacg cttatcgtag catctgcaaa tgcactgttt agtgtgatat ttgtcacgtt
    28681 aacagcatat gctttttcta gatatcgctt tgttggtcgt aaatacgggc tgattacatt
    28741 tttgatttta caaatgttcc ctgtattaat ggcaatggtc gcaatctata ttttgctaaa
    28801 tacaattgga ttattagatt ctttatttgg actaacactt gtatatattg gtgggtctat
    28861 accgatgaat gcctttttag tgaaaggtta cttcgatacg attccaaaag aacttgatga
    28921 atctgccaaa attgatggtg ctggccatat gcgtattttc ttacaaatta tgcttccatt
    28981 agctaagcca attttagcag ttgttgcttt gttcaatttt atggggccat ttatggactt
    29041 tatattacct aaaatactat taagaagtcc tgaaaaattc acattagcag ttggattgtt
    29101 caactttatt aatgataagt atgcaaataa tttcacagtg tttgcagcag gggcaattat
    29161 gattgcagta cctatagcaa tcgtattctt gttcttgcaa cgctacttag tatcaggttt
    29221 aacaacaggt gcgacaaaag gttagtttga aattgggagc ggggcagaaa tgataaagaa
    29281 ccactaatga atttattgtg tagtggctct tacaaattag ccacagctaa tatgtatcta
    29341 aaaatagcct agaacgttga ttgatgttta aggctcgaca tacgtactaa tgacgataaa
    29401 gatttaaagg aggacgttat gatgacgatt aaagttggaa tcattgggtg tggtggtatt
    29461 gcgaatggca agcacatgcc aagtttacaa aaagttgaaa atgttgaaat gatcgcattt
    29521 tgtgacgtag acatttcgaa agcagcgtgt gcggcagaag catacggtac tgacaatgca
    29581 aaggtttatg atgattacaa agcattgtta aaagatgaca cgattgatgt tatccatgtt
    29641 tgtacgccaa atgactcgca ttgtgaaatt actgtagcag ggttgcatgc cggtaaacat
    29701 gtgatgtgtg aaaaaccaat ggctaaaacg acagcagaag ctcaaaaaat gatagataca
    29761 gctaaatcaa caggtaaaaa attaacaata ggttatcaaa atcgcttcag accagatagt
    29821 caatttttat ataaatcagc gcaacgtggc gacttaggtg acatttattt cggaaaggca
    29881 catgctattc gtcgtcgagc agtaccaaca tggggtgtat ttctagacga agaagctcaa
    29941 ggtggaggac cattaatcga tatcggtaca cacgctttag atttaacgtt atggatgatg
    30001 gataattatg aaccagaatc agtgatgggt tcaacattcc ataaattaaa taaacagcat
    30061 gatgcggcaa acgcttgggg ttcatggaat ccagatgaat ttacagttga agattctgcg
    30121 tttggcttta ttaaaatgaa gaacggagcg acgatcattt tagaatccgc ttgggcgatt
    30181 aattctttag aagtggatga ggcaaaatgt tcattatcag gaacgaaagc aggtgctgat
    30241 atgaaggatg gtctacgtat tcatggtgaa gatatgggca cactttatac caaacacgtt
    30301 gaattggaaa acaaaggcgt cgacttttat gaaggtaatg aagtggatga agctgaagaa
    30361 gaagcaaaag cttggattga tgcagttgta aatgatactg aaccagttgt gaaaccggaa
    30421 caagcaatgg tagttacaaa aattcttgaa gcgatttatc agtctgcaaa atcaggcaaa
    30481 gcaatttact ttgaataaca tcatacggta aggaggcaca tcatgacaaa attaaaagtt
    30541 ggtgtgatag gtgttggtgg tattgcacaa gaccgtcata ttccagcatt gctgaaactc
    30601 aaagacacag tctcattagt tgcagtacaa gatattaata cagtgcagat gattgatgtt
    30661 gcgaagcgct ttaatatacc tcatgcagtt gagacaccta gcgagctgtt taaacttgtt
    30721 gatgcggtgg tcatttgtac acctaataaa ttccatgctg atctttctat agaggcattg
    30781 aaccatggtg tccatgtatt gtgtgaaaag ccaatggcga tgacgacgga agagtgtgac
    30841 cgcatgattg aagcggctaa taaaaattgc aaattattaa ctgtcgcata tcattatcgt
    30901 cacacagatg tggcaattac tgctaaaaaa gcaattgaat caggtgtggt tggaaaacct
    30961 ttagtagcac gtgtacaagc gatgcgtagg cgtaaagtgc ctgggtgggg tgtttttacc
    31021 aataaggcat tgcaaggtgg cggtagttta atcgattatg gttgccactt gttagactta
    31081 tctttgtggc tactaggtaa agatatggtg ccgcatgaag tgctaggaaa aacatataat
    31141 caattgagca aacaaccgaa tcaaattaat gattggggaa catttgatca cactaaattt
    31201 gatgtcgatg atcatgttac tagttatatg acatttgcca atcgagcaag catgcagttt
    31261 gaatgttcgt ggtctgcaaa tataaaggaa gataaggtac acgttagttt atcaggcgaa
    31321 gatggcggta tcaatttatt tccatttgaa atatatgagc cccgctttgg aactattttt
    31381 gaaagcaaag ctaatgttga gcataacgaa gacattgctg gtgagagaca ggcgcgtaac
    31441 tttgtcaatg cgtgtttagg gatagaagag attgtggtaa aaccggaaga agcacgcaat
    31501 gtaaatgccc ttatagaagc gatttatcgt agcgatcttg ataacaagag catacaactt
    31561 taatgattat tatatataat acaaaattct caatataaaa agaaggagtg cttttcaatg
    31621 aaaataggtg tattttcagt attattttac gataaaaatt tcgaagatat gttagattat
    31681 gtcgcagaat ctggattgga tatgattgaa gttggaacag gtggtaaccc aggagataaa
    31741 ttttgtaagt tagatgagtt gttagaaaat gaagacaagc gacaagcatt tatgaagtca
    31801 atcacagaca gaggcttaca aataagtggt ttcagttgtc ataacaatcc aatttctcca
    31861 gatccgacag aagcgaaaga agcggatgaa acgttacgta aaacaatccg tttagcaaat
    31921 ctattagacg tgccagttgt taatacattt tctggcattg caggatcaga tgataccgct
    31981 aaaaagccta attggcctgt tacaccttgg ccaacagcct actctgaaat ttatgattat
    32041 cagtggaatg aaaagttgat accatattgg caagatttag ctgagtttgc aaaagagcaa
    32101 gatgtaaaaa ttgccataga gttgcatgca ggatttttag ttcatacacc atatacgatg
    32161 ttgaagttac gtgaggctac aaatgaatac atcggtgcta acttagatcc tagtcattta
    32221 tggtggcaag gtatcgaccc aattgctgcg attcgcatat taggccaagc aaatgcaatt
    32281 catcacttcc atgctaaaga tacgtatatt aatcaagaaa atgtaaatat gtatggtcta
    32341 actgatatgc aaccatatgg taacgttgca acaagagcat ggacgttccg tacagttggt
    32401 tatggacata gtccatatgt atgggcagat atcataagcc aacttattat taatggatat
    32461 gattatgtat taagtattga acatgaagat cctattatgt cagtagaaga aggtttccaa
    32521 aaagcttgtc aaactttgaa atctgttaat atttacgaca agccagcaga catgtggtgg
    32581 gcataatacg aattcgaggt tagtctgaag tttgtctgaa gtaagactgg tggcagtgtt
    32641 gaataaatgc atatatcgcc aagccattgc caaaaatttc acaccttaaa tcaagttcat
    32701 tgtttgtaaa gaaggtgtac tttatataag tatatagcga tggtcatacc cattcacaat
    32761 aacaatcctc actattgaaa agagtaaata agcttttcaa tagtgaggta tatgataata
    32821 aaaaagcctg ttgtcacaat ggtcatagac acgacatact ttaaaggttt ctgaatataa
    32881 tatttcagaa cgcactttaa agatggacgt cgatggcgac taatgtgatg acaggctttc
    32941 atttttaaat attcattaat ttctcttctt gtttaatacg tacatataag aaatacgcat
    33001 acggtactaa taaaatagtt gtatatgttg cgtgtgttaa taataataca ccgattaatt
    33061 caggaatgat gtttaagaag taatttgggt gttttgtaat tttatataat ccagatttaa
    33121 taataggatg gttaggtaaa atgaataatt ttaatgtcca aataccacct aaagttttaa
    33181 taaccataaa taacatgata taagcaaaga ttaatataac taagccaata ccatttgcaa
    33241 agctaaatgt atctttatta ataaatgcct ctacaccagc caatacataa attaaaacgt
    33301 gtgttattgc taaaaacttc gaatttttaa cgccatattc aactgcaccg tctgctttta
    33361 attgttttga gtgattaata gatatcttta agctgacaag tctgatacag aaaaagataa
    33421 gtaatataga tagaatcatg atgtcctccg tcattatgtc atatgtataa gcgttgattt
    33481 tgacaacata aagtatttta tagataaaac ttgtcaaata ctattaacta tttattaatt
    33541 ttagtacata aatatgtttc taagtatgtg tttatgttca gtattttgga taatttaata
    33601 attttaagga tattaagcgc ttacaccgac gtgatatatt tgacttaacg aaaatgattg
    33661 aggtgacaga gatgaacttt tttgatatcc ataagattcc gaacaaaggc attccattat
    33721 cggtacaacg taaattatgg cttagaaact tcatgcaagc tttcttcgta gtgttctttg
    33781 tttatatggc tatgtattta attcgaaaca actttaaggc ggcacaaccg tttttaaaag
    33841 aggaaattgg attatctaca ttagaacttg gttatatcgg attagcattt agtatcacgt
    33901 acggtttagg gaaaacatta cttggatatt ttgtcgatgg acgtaacaca aaacgtatta
    33961 tctcattctt acttatctta tctgcgatta cagttttaat tatgggattt gttttaagtt
    34021 actttggttc agtaatggga ttattaattg tactttgggg acttaacggg gtgttccaat
    34081 cagttggtgg acctgcaagt tattcaacga tttcaagatg ggcgccaaga acgaaacgtg
    34141 gccgatactt agggttttgg aatacatcac ataatatcgg tggtgccatt gcaggtggtg
    34201 ttgcactttg gggtgctaat gtattcttcc atggaaatgt tatagggatg ttcattttcc
    34261 catcggtgat tgcattactt attggtatcg caacattatt tatcggaaaa gatgatccag
    34321 aagaattagg atggaatcgt gctgaagaaa tttgggaaga gccggttgat aaagaaaata
    34381 ttgattctca aggtatgaca aaatgggaga tctttaaaaa atatatcctg ggaaatcctg
    34441 ttatatggat tctatgtgtt tcaaacgtct ttgtatacat tgtacgaatc ggtattgata
    34501 actgggcacc gttatatgtg tcagagcatt tacactttag taaaggcgat gcagttaata
    34561 cgatattcta ctttgaaatt ggtgctttag ttgcaagttt attatggggc tacgtatcag
    34621 acttattaaa aggtcgtcgt gcaattgtag ctattggctg tatgtttatg attacatttg
    34681 ttgtcttatt ctacacaaat gctacaagtg taatgatggt taacatttca ttgtttgcat
    34741 taggtgcgtt aatctttggt ccgcaattat taattggtgt atcattgact ggttttgttc
    34801 ctaaaaatgc catcagtgta gcaaacggaa tgacaggttc attcgcgtat ctattcggtg
    34861 actcaatggc aaaagttggt ttggcggcta ttgctgatcc aacacgtaac ggtttaaaca
    34921 tctttggata tacattaagt ggatggacag atgttttcat cgtcttctat gttgcattat
    34981 tcctaggcat gattctatta ggaatcgttg ctttctatga agaaaagaaa attagaagtt
    35041 taaaaatcta atatcaatcg gattaaaagt atcgccaatt tattgcagta tagttggcaa
    35101 tcctgccccg acggcatgtg cgtgaagaga tgaaagatac tgcttctacc cttgcaaata
    35161 tatcatctct atgtctcggg gcagatcata attccctgtt atgaagtatc cttatttgcc
    35221 cgacttaggg tgactcaatg aatttactcc ctacaataaa gacatatagc ggtgtcaata
    35281 ttgtagggag tattgtttta tatttagact ctctaaaaag cggattgaaa gaaaagtgaa
    35341 aacttctcta tcagtccgct ttttcataga acaaaatgga tgcgccataa tcattagtta
    35401 tttgctaatc tattttgctt gcttacaata atcacttggc gacatttgta aatatttttt
    35461 aaaatgatag ctaaacattt tatactctga aaagcctact ttgtctgcaa tttcatagtg
    35521 cttataatgt cgatctaaca attgtagaga ttgtaaaata cgatagcgat ttaaataatc
    35581 gacaattgta ataccaacat gatctttaaa tgttcgcatc gcatacgatt cactaacatc
    35641 gatatgttga attaaatctg aaacagtcac tttcgtttga taagattgct taatttgatc
    35701 aacaatctga tttacataat aatcatcgta ttctactttt aataatggtt ggaaggcatc
    35761 atgacaaggt gctaagctac ggccgttctg tgattgttgc tctaataagg tacggacaag
    35821 tcttcctaaa ataacttcta attgtgcatg gtctactggt tttaataaat aatcaagaac
    35881 atgatgttga atgccggctt tcatatattc aaagtcatcg taactcgata atatgatgac
    35941 attacaatct agatgcgcaa tatcattgag taaatcgacg ccatttttac gtggcatacg
    36001 aatatcagta attactaatt ctggctgatg ttgttgaatt agtgataatg cttcaacacc
    36061 atctttagca gtgtatattg tattgaaatg atagtctccc caaggaatga tttgctttaa
    36121 accttctcga ataattcgct catcatcaca aataactacc ttaaacatct acattccccc
    36181 ttgaaagtgg tattttataa caaattaacg taccttgatt acgctttgaa aaaatatgga
    36241 gtcgtgcatg tgaaccatat tgaatcattg ctttattgtg taaatgattt aatcccaaat
    36301 gcttagtatc aaatacatca ttattaagag attggcgtac atattgcagg cgagatgacg
    36361 acatcccaat accattgtcg caaactaaaa catgtaaatt ctgacgtgcc aatgtcaggc
    36421 gtatagtaat gtccaatgac tcagtatctc taccatgttt aatagcattt tctatgagtg
    36481 gctgaagcat cattttacca attgtctggt gacgcgcttc ttcagaactt tcgatatgga
    36541 gcttaatcat gtcatcaaaa cggatgtttt gtatcgcaac atactgttca atgtagttca
    36601 actcttcgtt taattccact gtatgtgagt ttgtacgtaa tgagtaacgt aacatttgcg
    36661 ataattgttg gaccacagtt tgtgctaatt tcggagataa cgtaattaaa tattgtattg
    36721 tttgcatcgt attgaatagg aaatgaggtt ggaattggcg ttctatttcc tttaactgaa
    36781 tatcacgcaa gcgacgttct gtatgctcga tagaatggat cagttgctca tttgattcaa
    36841 ataaatcgta aatataatta ttaatttctt ctagttcact gttgtttttt aaaggcgtat
    36901 atgtacctag atgacgattt ttggcatagt aaattttttg aataatcgtt tcgatatctt
    36961 ttgtttgtcg tttagccata ttatctgcac taatgaaacc aaatattact agtaaaacaa
    37021 gaactacggc cataacaatt aacaacgtga taccatcttc aatgttttca tgtatatctt
    37081 tataaataat gagacgatgg tcagcatggt ttaattttac agattcattc ataaatccga
    37141 attgttgcgg tctatacttt tcacctatag taaaacggtc atcgttggcg tataaaatat
    37201 tgtcatattg atcaacgata agtgcgaatt gtcggctatc tttcttaatt tcacttaaac
    37261 gtggggtgtt agctatataa attttaagca tatatgtact atttttgaat ttaagctgat
    37321 gcgttgaaaa taaatacata tttttagtgt ttaaatgttc ataattattg gttataaact
    37381 gatttggtcc agataattca taataaagtg ttgcgggctg ttggtgtatt aattttaata
    37441 attcacgttt tgtagcggta acatcatgat gatttgttaa atcgagctct tgaaacgaat
    37501 tattatgctg tgtaataaac gtctgaattt gcttttcagt atgatgtaaa gatgactgac
    37561 tttcatcaac atgttgatga atcgtacgat gctcaatcca aatatagatg gcatagaagc
    37621 ttactagtcc aataataatc actaaaaata ctggaaaaat agtagacgca aataacgatc
    37681 gtctcaattg atgtctataa ggtttgtatg ccgtcattga atcatctcca aaaatttatg
    37741 atgcggaatg tccggtaatt tagatttcgg tattaaaggt atgttcttaa gattttcgat
    37801 agactgatcg ctttgttcac taacatcttt tcgaattgac ttggcatcga actctgcaac
    37861 taatcgttgt tgtactgagc ggcttgttaa atattgcact aactttttac gcttaggatg
    37921 agggtgtgca tttttaacta aagcaatacc atcaacattt aacattgttc cttcaattgg
    37981 ataaacgatt gatacaggat aacctttgtt tttccatgtg cgtgcatctt gttcgtagct
    38041 tagacctgca taatatttac cttttgcaac atcttcaatg actttagacg tctttgacag
    38101 ttgcatcgcg tggtcttgga attgatgcac atcacttact cgatgatgca tgctataaat
    38161 agcgcgcata tgttgatagc ctgtcgttgt tgtatttgga tttgagtacg caattttacc
    38221 tttaagtata ggttgtaata aatcttgata accttgaatc ttaatatctc cttgtaaatc
    38281 tgaattaact actataactg ttggcattaa tagaaagcta gtaacatatt tattgttcga
    38341 gcgataatcc tctaattgct gtgttacaga tgtatcttga tagggaacaa aatcttctgg
    38401 atgatcaatt gtttctgaca acacaccacc cataaagaca tcaccacgct ccgaaagatc
    38461 ttcattatgc aagtttgaaa gcagtacttg agtagatccg tgtttaattt caattttgac
    38521 atgctcttgt ttttcaaaat catttaaaat tggacgaatc aagtttgatt gatacggaga
    38581 ataaactgtt aatacatttt tatctgattc agagtgacgc gtattagtgc atgctgataa
    38641 aaaaatgaga aataatagca agatataaat ttttgatttc atgatatccc atcaattcta
    38701 tgtatatttt aatacaataa ttttagcaat aaatgacgca taagtaatgt taaatattta
    38761 gaaatgttta tagatgactt gttaagacgt tgcaaatgtt gtgatagcac ataatttttg
    38821 tttgtcaaga cgacttaccg aagctgtaaa gtcaaactgt tatattttat ttgaagctgt
    38881 tatataaaaa ttggcaagat attgaacggt tcaaaagtaa atttttacgt caataaaagt
    38941 atttaatcct gtctcttcat atataaaagt aaatctttct aagtattgat ttaacgctta
    39001 ttaacaatca ttttttataa acaaatatat actcctaaat taacttttaa agtaatgaaa
    39061 atagtgaaca ttataactgt tgtgtaacag aatgcaatta gcatattact gttacacaaa
    39121 ttagtacagt ttctatgttt tgacatacat ttgatgaaaa ttgtacataa tttatgtgaa
    39181 aaaaatcaca acaaacatgc tacaatgact atgaaaacgt taacatagca tttcaaattc
    39241 acaacattat acagatggag gcgtttagta tgttagaaac aaataaaaat catgcaacag
    39301 cttggcaagg atttaaaaat ggaagatgga acagacacgt agatgtaaga gagtttatcc
    39361 aattaaacta cactctttat gaaggtaatg attcattttt agcaggacca acagaagcaa
    39421 cttctaaact ttgggaacaa gtaatgcagt tatcgaaaga agaacgtgaa cgtggcggca
    39481 tgtgggatat ggacacgaaa gtagcttcaa caatcacatc tcatgatgct ggttatttag
    39541 acaaagattt agaaacaatt gtaggtgtac aaactgaaaa gccattcaaa cgttcaatgc
    39601 aaccattcgg tggtattcgt atggcgaaag cagcttgtga agcttacggt tacgaattag
    39661 acgaagaaac tgaaaaaatc tttacagatt atcgtaaaac acataaccaa ggtgtattcg
    39721 atgcatattc tagagaaatg ttgaactgcc gtaaagcagg tgtaatcact ggtttacctg
    39781 atgcatacgg gcgtggacgt attatcggtg actatcgtcg tgtagcttta tatggtgtag
    39841 atttcttaat ggaagaaaaa atgcacgact tcaacacgat gtctacagaa atgtcagaag
    39901 atgtaattcg tttacgtgaa gaattatcag aacaatatcg tgcattaaaa gaattaaaag
    39961 aacttggaca aaaatatggt ttcgatttaa gccgtccagc agaaaacttc aaagaagcag
    40021 ttcaatggtt atacttagca taccttgctg caattaaaga acaaaacggt gcagcaatga
    40081 gtttaggtcg tacatcaaca ttcttagata tctatgctga acgtgacctt aaagcaggtg
    40141 ttattactga aagcgaagtt caagaaatta ttgaccactt catcatgaaa ttacgtattg
    40201 ttaaatttgc tcgtacacct gattacaatg aattattctc tggagaccca acttgggtaa
    40261 ctgaatctat cggtggtgta ggtattgacg gacgtccact tgttacgaaa aactcattcc
    40321 gtttcttaca ctcattagat aacttaggtc cagcaccaga accaaactta acagtattat
    40381 ggtcagtacg tttaccagac aacttcaaaa catactgtgc aaaaatgagt attaaaacaa
    40441 gttctatcca atatgaaaat gatgacatta tgcgtgaaag ctatggcgat gactatggta
    40501 tcgcatgttg tgtatcagcg atgacaattg gtaaacaaat gcaattcttc ggtgcacgtg
    40561 cgaacttagc taaaacatta ctttacgcta tcaatggtgg taaagatgaa aaatctggtg
    40621 cacaagttgg tccaaacttc gaaggtatta acagcgaagt attagaatat gacgaagtat
    40681 tcaagaaatt tgatcaaatg atggattggc tagcaggtgt ttacattaac tcattaaatg
    40741 ttattcacta catgcacgat aaatacagct atgaacgtat tgaaatggca ttacatgata
    40801 cagaaattgt acgtacaatg gcaacaggta tcgctggttt atcagtagca gctgactcat
    40861 tatctgcaat taaatatgca caagttaaac caattcgtaa cgaagaaggt cttgtagtag
    40921 actttgaaat cgaaggcgac ttccctaaat acggtaacaa tgacgaccgt gtagatgata
    40981 tcgcagttga tttagtagaa cgctttatga ctaaattacg tagtcataaa acatatcgtg
    41041 attcagaaca tacaatgagt gtattaacaa ttacttcaaa cgttgtatac ggtaagaaaa
    41101 ctggtaacac accagacgga cgtaaagctg gcgaaccatt tgcaccaggt gcaaacccaa
    41161 tgcatggccg tgaccaaaaa ggtgcattat cttcattaag ttctgtagct aagattcctt
    41221 acgattgctg taaagatggt atttcaaata cattcagtat cgtaccaaaa tcattaggta
    41281 aagaaccaga agatcaaaac cgtaacttaa ctagtatgtt agatggttac gcaatgcaat
    41341 gtggtcacca cttaaatatt aacgtattta accgtgaaac attaatagat gcaatggaac
    41401 atccagaaga atatccacag ttaacaatcc gtgtatctgg ttacgctgtt aacttcatta
    41461 aattaacacg tgaacaacaa ttagatgtaa tttctcgtac attccatgaa agtatgtaac
    41521 aaaatttaag gtgggagcac tatgcttaag ggacacttac attctgtcga aagtttaggt
    41581 actgtcgatg gaccgggatt aagatatata ttattcacac aaggatgctt acttagatgc
    41641 ttgtattgcc acaatccaga tacttggaaa attagtgagc catcaagaga agtcacagtt
    41701 gatgaaatgg tgaatgaaat attaccatac aaaccatact ttgatgcatc gggtggcggt
    41761 gtaacagtca gtggtggcga accattgtta caaatgccat tcttagaaaa attatttgca
    41821 gaattaaaag aaaatggtgt gcacacttgc ttagacacat cggctggatg tgctaatgat
    41881 acaaaagcat ttcaaaggca ttttgaagaa ttacaaaaac atacagactt gatattatta
    41941 gatataaaac atattgataa tgacaaacat attagattga caggaaagcc taatacacac
    42001 atccttaact tcgcgcgcaa actgtcagat atgaaacaac ctgtatggat tcgccatgtc
    42061 cttgtgcctg gttattctga tgataaagac gatttaatta aactagggga atttattaat
    42121 tctcttgata acgtcgaaaa gtttgaaatt ctgccatatc atcagttagg tgttcataag
    42181 tggaaaacat tgggcattgc atatgaatta gaagatgtcg aagcgcccga tgatgaagct
    42241 gttaaagcag cctaccgtta tgttaacttc aaagggaaaa ttcccgttga attataaata
    42301 caattcagac cgaaaagaaa gcatatgcaa cttcaagagt gaaggggcat atgcttcttt
    42361 ttcaattgag tattgattat taacaagacg tagtaaatat atgagacaag tccaacaatg
    42421 gttgaaggaa gacgcatttg taagtagcta tgctgataaa gaatgtgatg ccttgttaaa
    42481 gatggggttc caatatcatc gaatgggtta ttatttgcta cttgcatacg aatatgagtc
    42541 ttttcaaatt tttattgacc ctgagtaatg aaaaatatta agatgaaact taatattaaa
    42601 gcaatgcgga gcgtgattat gaagagaatt agtaaagata tatgggcagt atttaaatta
    42661 ctgtatcaaa ataaagggcg ttttagcatt aatgccttac tattgcagtt aatcatgatt
    42721 tttattagta gtacatactt aattttacta tttaatatga tgttaaaagt agctgggcaa
    42781 agccaactta cgattaacaa ttggatggaa atcgtaagtc accctgccag tgtgatactt
    42841 cttattatat tcatattaag tgttgccttt ctgatttatg tagagttttc attgttagtt
    42901 tatatggttt atgccggctt tgatcgacag attattacat ttaaatccat ttttaaaaat
    42961 gcctttgtca atgtgcgtaa actcataggt gtaccagtta ttttctttgt catttattta
    43021 atgttaatga tacccattgc caacctagga ctaagttcag tattaacaaa aaatatttac
    43081 atacctaaat ttttaacgga agaacttatg aaaacgacga aaggtataat catttacggt
    43141 acctttatga ttgctgtatt tatattaaat tttaaattaa tatttacctt accgttaacg
    43201 attttaaacc gccaatcgtt atttaaaaat atgagactaa gttggcaaat tacgaagcga
    43261 aataagtttc gacttgttat agaaatagtt atattggaac tcatcattgg tgcgatttta
    43321 acattaatta tttcaggagc aacatatctt gctatttgtg tagatgaaga aggagataag
    43381 tttttagtct catcaatttt atttgttgta ttgaaaagct cattgttctt ctattattta
    43441 tttacgaaat tatcattaat cagtgtgtta gtactgcact taaaacaaga gaatgtatta
    43501 gaccagccgg gcttagaatt taaataccca aaaccgaaac ggaagtctag gttctttata
    43561 atttcaatgg tgcttgcagt gacgtgtttt atcggttata acatgtactt actttacaat
    43621 aatactatca atacaaatat ctccattatt ggccatcgtg gtttcgaaga taaaggcgtt
    43681 gaaaattcta ttccgtcatt gaaagctgct gcaaaagcga atgtcgaata cgttgagtta
    43741 gatacaatta tgacgaaaga taaacaattt gttgttagtc atgataacaa tttgaaacgt
    43801 ttaacaggtg ttaataaaaa tatttctgaa tctaatttca aagatgtcgt cggtttgaaa
    43861 atgcgtcaaa atggacatga agcaaaactt gtatccttag acgaatttat tgaaacggct
    43921 aaacaatcaa atgtgaaact actagttgag ttaaagccac atggtaaaga accagcagat
    43981 tatacacaac gtgttattga tattttgaaa aagcatggtg ttgaacatca atatcgtgtg
    44041 atgtcattgg attatgatgt gatgactaag ttgaaaaaag aagcgccata tctcaagtgt
    44101 ggttatatca ttccgttgca gtttggtcat tttaaagaaa catctttaga tttctttgtc
    44161 atcgaagatt tttcttattc gccaagactt gttaatcaag cacactcgga aaataaagaa
    44221 gtctatactt ggaccattaa cggcgaagaa gatttaacga aatacttaca aaccaatgtt
    44281 gatggtatta tcacagatga cccagcatta gctgatcaga ttaaagaaga aaagaaagac
    44341 gaaacatact tcgatcgttc tataagaata ttatttgagt aatataaaca aagacctcta
    44401 aagttatcaa gatgatatct tcagaggtct ttttaacgtt gccatctatg gtataggcaa
    44461 tcgtttcatt cgtttataat catatgacaa ggatttataa ggaaatttgg cgtcacaaac
    44521 acttacatga tttattggtg aattattaat tgttttgtga atgcaaaggg ttagaaattg
    44581 aatcgtaaat actttctaat ctatgtttcg ctttagtcat ttgatccaaa tttttagtgc
    44641 gtatagctga tttagcaata tagtgtgcag ctaaaatatc gcgtttttga tacgcatcta
    44701 aatttaggtg cgataattta tttaagtcag tgtttgctat taattcatgt aattgatcta
    44761 caagcgcttg atgttgatac gtatgtgatg tagtttcaga tttgcttgct aatttaatac
    44821 cagtcgtatc aaggagcgcc gctttaatac cagcaactaa atatgttttg attttcattt
    44881 gtgttgtcat gctttgttac tcctttgatg tacattaatc aaaaaaatta tacactattg
    44941 tatattgcaa agctaattaa ctataacaaa aagatagtta atgctttgtt tattctagtt
    45001 aatatatagt taatgtcttt taatattttg tttctttaat gtagattggg caattacatt
    45061 ttggaggaat taaaaaatta tgaaaaagca aataatttcg ctaggcgcat tagcagttgc
    45121 atctagctta tttacatggg ataacaaagc agatgcgata gtaactaaag attatagtaa
    45181 agaatcaaga gtgaatgaga acagtaaata cgatacacca attccagatt ggtatctagg
    45241 tagtatttta aacagattag gggatcaaat atactacgct aaggaattaa ctaataaata
    45301 cgaatatggt gagaaagagt ataagcaagc gatagataaa ttgatgacta gagttttggg
    45361 agaagatcat tatctattag aaaaaaagaa agcacaatat gaagcataca aaaaatggtt
    45421 tgaaaaacat aaaagtgaaa atccacattc tagtttaaaa aagattaaat ttgacgattt
    45481 tgatttatat agattaacga agaaagaata caatgagtta catcaatcat taaaagaagc
    45541 tgttgatgag tttaatagtg aagtgaaaaa tattcaatct aaacaaaagg atttattacc
    45601 ttatgatgaa gcaactgaaa atcgagtaac aaatggaata tatgattttg tttgcgagat
    45661 tgacacatta tacgcagcat attttaatca tagccaatat ggtcataatg ctaaagaatt
    45721 aagagcaaag ctagatataa ttcttggtga tgctaaagat cctgttagaa ttacgaatga
    45781 aagaataaga aaagaaatga tggatgattt aaattctatt attgatgatt tctttatgga
    45841 tacaaacatg aatagaccat taaacataac taaatttaat ccgaatattc atgactatac
    45901 taataagcct gaaaatagag ataacttcga taaattagtc aaagaaacaa gagaagcaat
    45961 cgcaaacgct gacgaatctt ggaaaacaag aaccgtcaaa aattacggtg aatctgaaac
    46021 aaaatctcct gttgtaaaag aagagaagaa agttgaagaa cctcaattac ctaaagttgg
    46081 aaaccagcaa gaggataaaa ttacagttgg tacaactgaa gaagcaccat taccaattgc
    46141 gcaaccacta gttaaaattc cacagggcac aattcaaggt gaaattgtaa aaggtccgga
    46201 atatctaacg atggaaaata aaacgttaca aggtgaaatc gttcaaggtc cagatttccc
    46261 aacaatggaa caaaacagac catctttaag cgataattat actcaaccga cgacaccgaa
    46321 ccctatttta aaaggtattg aaggaaactc aactaaactt gaaataaaac cacaaggtac
    46381 tgaatcaacg ttaaaaggta ctcaaggaga atcaagtgat attgaagtta aacctcaagc
    46441 aactgaaaca acagaagcat cacattatcc agcgagacct caatttaaca aaacacctaa
    46501 gtatgtgaaa tatagagatg ctggtacagg tatccgtgaa tacaacgatg gaacatttgg
    46561 atatgaagcg agaccaagat tcaacaagcc aagcgaaaca aatgcataca acgtaacgac
    46621 aaatcaagat ggcacagtat catatggcgc tcgcccgaca caaaacaagc caagcgaaac
    46681 aaacgcatat aacgtaacaa cacatgcaaa cggccaagta tcatacggag ctcgtccgac
    46741 acaaaacaag ccaagcgaaa cgaacgcata taacgtaaca acacatgcaa acggtcaagt
    46801 gtcatacgga gctcgcccaa cacaaaacaa gccaagtaaa acaaatgcat acaatgtaac
    46861 aacacatgca gatggtactg cgacatatgg tcctagagta acaaaataag tttataactc
    46921 tatccataga catacagtca atacaaaaca ttatgtatct ttacaacagt aatcatgcat
    46981 tctatgatgc ttctaactga attaaagcat cgaacaatcg gaagcatatt tctaaattat
    47041 ttattcatta tagtcttaaa cataacatga cctaatatat tactaaccta ttaaaataaa
    47101 ccacgcacat ctatgtgaca tacgacaatc acagcaataa taattgcttt agaaagtcgt
    47161 accgaactgg aacttacaag tctagttcga acacacactg atgtgagtgg ttttctttat
    47221 tttaaacatg aacaatcaga taagttacta gtagtagcaa atattattaa atcaaagggc
    47281 ttcgattcat aaaatttaaa acaatgatta aaattagacg tgtaaatgtt aaattctaaa
    47341 acggaaatac ccgctatcct attaaaccac tttttggttc aatcactata tttcacacag
    47401 tttcaataat aaaacgaatc tgcttcaatc cgcttcaact tcagcctact tcattcaata
    47461 acaaaacgaa tccgcttcat tcaaaatcaa ccattctaac gcacatactc aaatatagca
    47521 gctgcaccca taccgacacc aatacacatc gtaaccatgc cgtaacgact atcgggacgt
    47581 ctacccattt cattaagtaa acgcgcggtt aacattgcgc ctgtagcacc taatggatga
    47641 cctaaagcaa tagcgccacc attcacattc gtacgtgata tatctagacc tacttcttta
    47701 atagatgcaa tcgtttgaga agcaaatgct tcgttcaatt cgattaaatc aatgtcttca
    47761 acagatagat tgctgagtga caatacttca ggaatcgcat atgcaggccc aatacccata
    47821 attttcgggt caacgcctac tgccttataa ccaacgaatc gtgcaatagg tgtcacgcct
    47881 agttctttca ctttatctcc agacattaaa actacaaatc ctgcaccatc agaaagtggg
    47941 gcagatgttc ccgcagtcac agtgccgtca gctttaaata ctgtacgtaa tttggctaat
    48001 gcctccatcg tggtgtcagg tcgtataaat tcatcttggt caaagatatt tgtgtgtact
    48061 tttggtccta cgtttgtata ttcaactgag tttacttgta ttggaataat ttcatctttg
    48121 aaccgaccat cacgttgtgc gtcataggca cgctgatgac ttctgacagc ataagcatct
    48181 tgatcttcgc gtgatacgtc aaattgggat gctacatttt cagcagttaa acccatagga
    48241 tatgacgcac ctatatcatc atattgtaag gtaggattgt ttgtgggctc gttgccaccc
    48301 attggtacgg cactcatcaa ttcaacgcca ccagctacaa gtatatctcc ttgacccgcc
    48361 ataatttgat tggctgcaat cgcgatggtt tgtaatcctg atgagcagta gcgattcact
    48421 gtttgacccg gtacagtgtc agataatccc gtacgcaatg caatcgttcg tgcaatgttt
    48481 tgtccttgta atccttctgg aaaagccgta ccaacaatga catcttcaat catattctta
    48541 ttgaattttc cgtcaatacg tttcaatacg ccttgtaata ctttggctgc gacatcatca
    48601 ggtctttcgt ggaataatgc gccttgcttt gctttcgctg cggctgaacg cccataagct
    48661 acaatgtatg cttcttgcat ggttatcatc ctctcttaat gactatcttt taattacgta
    48721 atggcttacc agtttttaac atatgcgcaa ttctttcata tgatttttta gattttagta
    48781 agtcaataaa gccaattttc tccaacgatt gaatgtaacg ttgattgata aatgtatttc
    48841 ttggtaaatc accacccgat aaaattgtgg cgatatttaa ggcgatatga taatcatggt
    48901 cgctaataaa atgaccgtgt ctttgcgcat ctaattgtcc ttggatcaat gctttgaagt
    48961 cttcgcctaa agcgatatat tgatgtctag gattcggaat atagtttgtt tctgcttcat
    49021 atttcgcacg tttgagcgca acttcgacac gttgtgctgt attgaaaata atcgtatctg
    49081 tatcacgtaa ataaccataa cgacgtgcct caaaggcatt tgtagagact ttcgcaaatg
    49141 cgatattcgt cagtactttt gtcatggaag cttgtttgtc atcaaactta tgcgatgtgc
    49201 gtaatatgcg atcagccatt tctgcaaggc cacctccact cggtaataag ccaacacctg
    49261 cttcaacaag accgatataa gtttcacttg cagcgacaac aataggtgag taaagtacaa
    49321 gctcacaacc accgcctaag gcacgacctt gaacagctgt gactactggt ttcaaactat
    49381 acttcaaacg attaaagcta taatgtaatt tatcaattga ttgtgcaacg acatcatcta
    49441 caagaccgtc ttcatgcgcc tttttcatta agaaaaggtt agcgcccaca ctgaaattat
    49501 taccatctgc ataaataacc atacttgtgt aatgttcatt ttccagtaaa tcaatcgcat
    49561 caactaacgc atcgttgaat tcatcggtaa tgacattatt tttactttgt aatttcagta
    49621 acagttgatc attatgagtt acggaaagtt tggcatcacc tttatcccaa agttcatctt
    49681 ttacgaagtg agaaacaggc gttgcatatt caatggtctc atcttgttta taaaagccac
    49741 cgtctaaatc actaatccat tgtggtaagt ctccaagttc gtcttccata cgtgttttaa
    49801 cacgttcata tcccattgca tcccataatt ggaatggacc aagtttccag ttgaaccccc
    49861 agacaagcgc acggtctatg tctcggaaat catcggtagc tttaggtaca ttgatagcag
    49921 agtaatagaa attattacgt aatgtctccc ataaaaatag tcccgcttcg tcttgcgcat
    49981 tgaatatgac atcaaggtta tgcactaagt ctttattaaa ttcatttaaa attggtaatt
    50041 gtggttgcga tacaggtaca taatcttgtt tttcaacatc gtaaacaagt cgagctttag
    50101 tttctttatc ctttttgtaa aatccttgtt ttgttttacg tccgagtgcg ccattttcat
    50161 acaacgtatt tacaattttg acatcatgaa aataaggtgt ttcttcaggt acttgttgca
    50221 tgcctttaat tacagacact gcaatatcta aaccgactag gtcagatagg gcatatgtac
    50281 ctgttttagg acgaccaatc gcttgcccag ttaaagcatc cacatctaca atgcttaact
    50341 tgtgttgctc ggtgcgatac ataatatcat tcattgtttg cgtgccgact ctatttgcga
    50401 caaagccagg cacatcattg acaacaatga cacctttacc taacacattt tgcgcgaaat
    50461 tttttacatc taatataata gattccttcg tgtgtgacgt aggtattaac tccactaatt
    50521 tcataatacg tggtgggtta aagaaatgta gaccaaagaa tcgttcttga tccttctcgt
    50581 taaatgcttg agcaatcgca ttaattggaa tacctgatgt atttgtagcg aataaagcat
    50641 cttctttagc atgttgtaga actagttgcc aaacagcatg cttaatttca atatcttctt
    50701 tgactgcttc gatatataaa tcagcatcat catttaccaa gtcatcatca aaattaccat
    50761 atgttaaatg actcgctaga tttaagtcga atagtagcgg tcgtttctta tctgtaattt
    50821 tatcgtaaga ttttttcgca atgagatttg gatcgttttt gtccactaca atatctaata
    50881 gttttacttt aagtccagca ttcacaaaga gtgctgccag ttgagcgccc attgtgcctg
    50941 cgccaagaac ggttacttta ttaattgtca tagtgattcc tccaatttag ttgaggataa
    51001 gataaccatt aagataattg gaataacgtt gctattttat aaaattaatt aagtatcttt
    51061 gacagtcatc ttagcctctt atttaaggaa aaagctttat gcttaaaata agtctttttt
    51121 tagtgaaatt actgcatcac atataattct ttgctattta tacgaaagca gaatctccag
    51181 tcaaagcgcg tccaattact aaggcattaa tttcatgtgt gccttcgtat gtgtaaatcg
    51241 cttctgcatc agagaagaaa cgtgcaatat cataatcgtc agctagtatg ccattaccac
    51301 ctgtaatacc acggcccata gctactgttt cacgcaaacg taaggcattc atcatcttcg
    51361 cggttgaagt tgcaacctca tcatattcac catgtgcttg catattagct aattgagcac
    51421 atgttgccat tgcttgagct aaattacctt gcatcattgc tagcttttct tgtattaact
    51481 gatatttact aattggtttg ccgaattgct tacgctcagt gacataatct aatgtggcac
    51541 gtaaagcgcc agccatacca cctgtagcca tataagcaac gcctgctctt gttgaataaa
    51601 gaattttggc aatatcttta aagcttgtta tgttttgtaa gcgatccgct tcatctactt
    51661 tgacattagt taatttaatt agggcgttag gaacaatgcg aagtgcgatt ttattatcaa
    51721 tgacttcaat atcgacgcca tcttgttctg gtctgactac aaagcaatgg ggtttgccag
    51781 tttctttatt tactgcgaat actggaatga catcagatac atgtgcacca ccaatccatt
    51841 tcttttcacc attgataacc caagtatcgc cctggcgttc agcgactgtt tcaagacctc
    51901 ccgcaacgtc cgaaccgtgt tctggttcag ttaaagcaaa gcatgtacgc agttcatgtg
    51961 actgtaattt aggtacatat ttcgcaattt gttctttgct acctccgaaa tagaaagtgt
    52021 tatgccctaa accttggtga acaccgagta gggtagctaa ggaaatatca aatcgcgcga
    52081 gtaggtaaga catgaaaaac tgaaatagtt gactaggcat tttggcgttt ggacgatcct
    52141 tgtatagtaa tggattgtta aaataattta attctcccag atctttaaaa tagtcctcgg
    52201 gtacagtagc gtctatccaa tgttgattaa tattttcacg gtacttactt tctagcaatg
    52261 aatctacttg ttgtaaaaat tcgacttcac cgtctgttaa acctttagca atactaagta
    52321 catcttcagg aaataatgtt tttaagactg tttctttttc aaatgtcata taaattcctc
    52381 ctaaaaataa tatgaatact aatgtgaaat gcatttaatt caaaacaaca cgctttattt
    52441 gtaaacgctt acactaaatg ctaaaaattt tttatcacct ttaaagtgtt tgtgagactt
    52501 tgtcattcat catttgtcga atcgcaagtt tatctggttt ctgcgtactg tttaacggca
    52561 tatgtgtcac tggtacatac attcttggaa ctttataacc tgctaaacga cttcgcatat
    52621 gttgatctaa aatttcagcg taatgaggtt catcttcacg aagtataatg gctgcagcaa
    52681 ttgactcacc atattttgga tgatcatagc caacgaccac acaccggtct actagtggat
    52741 gctccgctaa agcattttcg acttcggatg gtaagacatt ttcgccacca gttatgatta
    52801 attctttttt gcggtcaata ataaatatat cgccatcgtc gtccatcttc gctaaatctc
    52861 ctgtcaataa gtaacgacca tgaaacgctt tagcagtctc tgctggttta ttccaatatc
    52921 ctggcgtgac atttttagcc ttaattgcaa gttcgccaat ctcaccagta ggtacttctt
    52981 caccgttatc atcaaggata cgtgcatcaa caaacatgac tgctttacca atactcattg
    53041 gtttacgttt tgaattttcc ggtgtattaa caagtacaag cggtgcttca gttaagccat
    53101 agccattaat aatgtttatg ccatattgct taaaagctgc ttggatactt ggtaatggtt
    53161 gtgaaccacc ttggatgata taatccatag ctttaaaatt ttcaggatta aaattactag
    53221 cacgtagcgt actataatac attgtcggaa tcatgataat aaatgtaggg tgatattgtg
    53281 caatcatgtc attcaattct tcgccgttaa agtaacgttg aagaataagt gttccacctg
    53341 acattaatac tggtaataca gtatcgttaa accctaaaac atggaacatt ggtgttgata
    53401 caatcgtaat atagtttgaa ttgaacttgt acgttagctc taagtttgcg ccgttatgaa
    53461 caaatgattc atatgagaac atcacacctt taggtgatcc ggttgtacca cttgtataaa
    53521 ttaatgctgc aagatcttgt ggttcaacag gtgttgcttg aaaaggttgg tgataatctg
    53581 gatttacgat ttcatcatat tgcgctacat caatatccat atgcaataag ttttggtcaa
    53641 tatcggtgag tgaacttaaa tgtttttcag catagaagag cagttttaat tgtgcatctt
    53701 ccacaatggc tgcaatttct tttgggttaa gccgccaatt caatggtaaa aaaaccgcac
    53761 ctgttttaaa acaagcaaat aataaatcta atattgcaat atcatttggc gcaaaaatac
    53821 cgataacatc gcctttttta acaccttgag atgttaaata atgtgccata ttatcagcgc
    53881 gtgcattgag ttgttggtat gtccaagatg tttgttttgc gtgatcaata acggcaggct
    53941 tgtcatcatc gaagtctgag cgcgttttta tccaatcgaa attcattagt ataccccctt
    54001 tagcttcact ttcatacttt atgaattgat tgtttaagtt ctccccattt ttctttgtaa
    54061 atgctggtat caattaattt taaatgatca gcaataattg gtttaaaagc catttgattc
    54121 aaaatatctt tatgcaaatc aagacctggt gcaatttcaa ttagtttcaa gccttgattg
    54181 gtgagctcga atactgcacg atcagtaaca aaatagactt cttgctcgag tgattgtgaa
    54241 tattgtgcat taaagtcgat atggctcaca tctgatacaa atttctggtt ttgtccttca
    54301 gtttcaatgt ttaatcgttg attatgacat gagacatgac tgccagctac aaaagtacct
    54361 gaaaagataa ttttatttac agattgtgta atgtctataa agccgccaca tccatttagt
    54421 cggtcattga agtaagacac gttgacattg ccgtattgat caacctcagc aaagctaaga
    54481 taggcaactg atacaccatt gttataaata aaatcccatg ctcgatcatg aggcatgcgc
    54541 acatctgcat tgtaattcat accaaaatgt tcacgactcc caacgaatcc accgaaaatg
    54601 ccaacatcta gaatcggttg cacatcatgt tcaacacatt cttcatgcaa taaattagag
    54661 agttcattat tgatgccata accgatgcta attgtatcgc cataagttaa aaactgagca
    54721 gcacgtcgga gaatcaactt gcgactatta aaaggtaatg cgggttcagg tattccatca
    54781 atccgttctt ctccagacaa ggctggtaaa taatgactct gaattacttg gcggtgattc
    54841 ttttcatctt ctgtgacgta tacataatcg acaagatttc ctgggataac aacttcattc
    54901 ggctttagtt gatagtcgtc aactaaatct ttaacttgta caataacttt cccatgattg
    54961 gctttcgcgt ttaatgcgac atgataacac tcgctcaagt aagattcttg agttaaataa
    55021 atgttacctt gttgatctgc gtatgttcct ctcagtagtg ccacatcaac gctagggaat
    55081 gtgtagtgta agtatgtttc atcgtcgata gttactaatg aaactaaatc atcagttgtt
    55141 cgtgtattta ctttaccgcc accgtatcta ggatcaacag ctgtgtttaa tccgatttta
    55201 gtaataactc ccggtaataa ttgattactc tgacgataat gagttgcaat gataccttgt
    55261 ggcaaaaaat aagcttcaat gtcattattt ttcattgctt gtgccgtttt ggaagaagcc
    55321 gttaaaatgc tcataatgac acgtttaatc atgcgacgtt ctataaaatc atctaaatcc
    55381 ggtgcgacac ctaaactatg gatatcattc gctaatataa atgttaaatc attgggcgta
    55441 tgatatgtgt catgttgcgc taacacagca cgtagaactt cggcgggtaa gttggctaca
    55501 gctaatgctg gtaaaccaat cacatcatca tctttaatga tatgttgtaa gtcgtgccat
    55561 gtgatttgtt tcaagcaagt cacctccatc acatttgata aaatatagcg tttttacact
    55621 ttgtgtaaac ccttacaaga aatataacat aacgacgttt aaaatcaatt agaaatatct
    55681 ttttattctg ataatagaca cagtatagac acattttaat agtcgataac aattgtaata
    55741 tcaagggttt gtaatgaatt gaatatcatt aaaatactta tataaaaata ttgctcggaa
    55801 tataaaaagt aacccatgtt tatttaaaaa atgcaaagac taattatcat tttgatgcat
    55861 cagtaaggcg agggtgatca gcgtacaatt agtggtgtat aatgtaactg ataaaatcat
    55921 tggagagttg aaggagatgt taaacatatg agagtcaatg ttttacaaca tacaccaaat
    55981 gaaggtgttg gatacatagg tgagtgggcg aaggaaagag ggcacgaagt ctatatttat
    56041 catccatata tgaatgatgg cgtgctacct cttgcggaag aaacggatat gttagtgata
    56101 cttggtgggc caatgaatcc agacgatgag atgccttgga ttcaatcaga gcgccaatta
    56161 attttagtat tgatggcaca aaacaaacca atttatggtg cttgctttgg tgcacaacaa
    56221 atttcgaaag cattaggtta tgcggtgtta aaatcaccag taaaggaagt aggttgggat
    56281 gatgtttact tagaatcaga aatcattccg aatataccaa gtcgattgaa ggcactacat
    56341 tggcatgaag atatgtttca aataccgcaa caagcagaat tattattttc ggggaaccat
    56401 attagaaacc aaggttttat catcgatcat catgtgattg gattacaatt tcattttgag
    56461 cctggtccag acgatgtcag agaaatagta ctaaatgatg cccaatacat caatcattct
    56521 attttagagc aatcggcgga ggacatatta gcctttgagg tgcctaaaca gaataggcag
    56581 gccattttcc gaatacttga ttttattact caagcgtaag atgtgagttg tattttaaaa
    56641 tgagtaaaaa aaacgaatgt acacaatcga acaaagtagt tatataaata tatggaaaat
    56701 ccatcaatat taaattaaaa tagttttatt atgaaaagtg aaagtaggtt agtctatgga
    56761 aggtcttaat catcaaagaa atacagaaaa agaagagaca acacaaacgc aatcagttgc
    56821 acctaataca ggtgaagaag ggatgtcatc agcaagtaca caatctacta agacatccga
    56881 catacataat gaatctatcg ataaacaaat ggaagccaaa gcgcatgaaa cagcgcaaaa
    56941 tacagattta aaaaatgaag caagaagttt atttgataat gcaaccaaat caatcggtag
    57001 actagctggc aatgatgaaa gcttaaatct taatttaaaa gatatgtttt ctgaagtatt
    57061 taagccacat actaaaaacg aagcagatga aatatttatt gcgggtactg ctaaaactac
    57121 gccagcaatt tgtgacatat cagaagaatg ggggaagcca tggctctttt ctcgagtatt
    57181 catcgctttc acagtaacat ttattggatt atgggtcatg gcagcaattt ttaataacac
    57241 taacgcgatt ccgggtctca tttttatagg ggctttaaca gtaccattat cgggtttgtt
    57301 ctttttttat gaatcaaatg cgtttaaaaa tattagcatt tttgaagtta ttatcatgtt
    57361 ctttattggc ggcgtatttt cattactaag tacgatggta ttatatagat ttgtcgtttt
    57421 tagtgatcaa ttcgaaaggt ttggttcttt aacatttttc gatgcatttt tagtaggatt
    57481 agttgaagaa actggaaaag cgctcattat tgtttatttc gtcaataaat tgaaaacaaa
    57541 taagattttg aatggattat taatcggtgc tgctattggt gcagggttcg cagtttttga
    57601 atcagcaggt tatattttga atttcgcttt aggggaaaat gtcccattat tagatattgt
    57661 cttcacacgt gcgtggactg cgattggtgg tcatttagtt tggtcagcga ttgttggtgc
    57721 tgcaatagtt attgcgaaag aacagcatgg ctttgaattc aaagatatat ttgataaacg
    57781 ctttttaata ttctttttat cagccgttgg tttacatggc atttgggata catctttaac
    57841 tatacttggc agtgatacgt tgaaaatatt tattttaatc gttattgtgt ggatacttgt
    57901 attcatttta atgggggcag gtttaaaaca agtgaattta ctgcagaaag aatttaaaga
    57961 acaacagaaa aaagtagacg aataataatt aaagcttatg ttgcacatat gtttgtgaca
    58021 taagctattt ttataatttg tctttaaaag agtggaagtg gaatactttt tggagttaaa
    58081 aaagtgtttc acgttaaaca aatagtgaca attaggttta tataaaatga acatgattcg
    58141 ctgaaagtat gtaataatca ttttattgaa attcatcaaa cagaaagtaa tacaatcata
    58201 taagcaaatt aaaccacgcc atcatcatct agaatgactt cggcgtggtt tttattgaag
    58261 cggggctgag acataaatca atgtcccaca ttcccttatc gttcaatcgt agttcgataa
    58321 tcgattaaat agataccttc aggtgttact ttataatttt taaccttaga gttagcagca
    58381 actatttgat cgttgtaagc aatataactg ttaggtacat ctcgacttga taatttaata
    58441 atatcattag aaatattgtg acgttcctta acatctacag tatgattcaa ttgattaatt
    58501 aaatcatcaa cgttgttatt attgtagtct cctttattaa tagcaccatc ttttttatat
    58561 gcttgattaa agaaatagcc tgtatctcca cgaggaatcg ttccgaaact atacattgtt
    58621 gcatcccatg cagaacggtc ttttaagtaa tcttctatgt catcaacact tttaatgtcg
    58681 atttcaatat ttgctttttt agcatctgat tgtaatactt gcgcaatttt cgatagctct
    58741 ggacgaccgt catacgtaat taacttaatt tttaaagggt gttcttttgt gtaaccatct
    58801 ttagctaata acgtttttgc ttgttcgata ttttgtttgg ttaacttagg ttctttaata
    58861 tatggaattt tatcattaaa tagactcgtt gcaggtttcg cgtaaccttg ataaatatga
    58921 tctgcaatac cttgtctatc aatgatatga tctaatgctt cacgaacgga tttagtcatt
    58981 tttttattag tatgattata cataagtaaa gaagttctaa atccagattc ttttgacact
    59041 tttaaatttt gattattttc tatgtcttga actttattaa ctgggacatc agttattaaa
    59101 tcatcttttt gagattctaa atttctgacg cgattattgc cgtcttcttg gtacgtcaca
    59161 gtaatatgat caagtttcgg tttaccttgc caatagtcct taaaattcga caatgatatt
    59221 tttcgagatt gcttataatc tttaatttgg taagggcctg taccaacagg agtttgatta
    59281 acatctgatt tagcatctgt atcataaatt gccataaaag gattagctaa ttcagataca
    59341 agttcagggt aagcggaact ggttttaatt gttagttttt gacctttagc ggtaattgat
    59401 gatattggta atgaatattt gaccaagtcg ctttttttca tgctattttc aaggctagat
    59461 ttcacttttt ctgcagtcaa tttttgaccg ttttgaaatt taatattatc ttttaattct
    59521 atatctaacg ttgtatcatt tggttgatga tacgatttca ctaatacttt ttctattttt
    59581 ccttgatcat ttgttttaaa taatgattct gcagcaccga tcttaactgg tacatctgtt
    59641 tcataaggtg caatagactt tgtttttaac ggtaacgaaa tatttaagtc tttgccagat
    59701 gaatgcattg agccacatcc tgataacact aatactgctg aaaatatagt tgctagtctt
    59761 ttaaacttca tttcattaac actctctttc taattactat gtaaaaccca acaattaata
    59821 ttttaaaact ttattttgtt gaagtaaaat gttgttcaag ttcagtaatt attaaagttc
    59881 aattaattgt agtaattaca ctttttaaaa ataaatatta gaaatgaagt tagcgacatg
    59941 tatagtgatc cacgataaac atatatatct aagtattgag caactgctgt agtactacag
    60001 cttggttatg tttagtatct tttgctgaat ataacaatag aacatgatga tgttgattta
    60061 caattgcctt taatttttca aaagcttctt tttgcgtctc attgtcacgc aattcttttt
    60121 cgtatttttc tttaaaagca ccataaagtt taggatcatg ttggaaccat tgtcgtaact
    60181 cagtagaagg ggcaatgtct tttaaccaat aatctaggtt agcagttctt ttcgaaatac
    60241 ctcttggcca gactctatcg actaggatac gaatagcgtc tgtattatct ttattgtcat
    60301 aaatccgtcc aatatctacg gtcatcttgt gaactccttt cttatgaaat tcagtgagca
    60361 tacatcaatg catgttgtgg tgggacgacg aaataaattt tgcgaaaata ttatttctgt
    60421 cccactccca attaaaaaac aaccatgaca aagtaaagtc atagctgttt ttgtatagat
    60481 gtcatttatt tttacgttta gttaaatact tcaaaccaac tgcaaagacg gtgtaaccgg
    60541 caagtgtttg aatcaatgtt ttgattaaat tgttattttt gacaacaata tttgcggtaa
    60601 caatactcgc gaaatataat gcaaatactt taacgtaacg ttgattaagt ttcatatgag
    60661 cactaacctc ttttctcagt tatactacaa cgcttagtct tggaataaat gtttcgtagt
    60721 gtacgcgatc catatcgtaa tttaaagatt taagtgcttc gatcatagat tgtaagaatt
    60781 ttgtaccacc acagatataa atttcaggtt tatttgctaa aaatgctttt aattcttcag
    60841 caccgatata accttgttcg ccttttaagt gggtatataa tttagcgttg tcataatgat
    60901 ttgcaatact gttgaagttg tctttgaaag gtaaatgttg ttcattttca gcaacttgaa
    60961 ccatctgagt atctaaacct ttggcagagg cagcttcata cattgctact aaaggtgtga
    61021 cgccaatacc tgaacctaag aaaagttgtg gttcagtcgt attctctaat acgaatccac
    61081 ctacaggtgc agctaaatta atcatatcgc cttctttaat ctcatcgtgt aaaattgttg
    61141 aaacttcgcc ttcatgttct gttgtgacat cacgtttaac gccaaaggtt aaatgatttt
    61201 tatcacctga tacgatagaa tagtgacgtt tagctctata tggaagttta tcactagaaa
    61261 catcaactgt gatgtattgg cctggtacat attgactaaa gtcatatgca tcagtttcaa
    61321 ctgtaaatga tttaatgtct tcagattctt gtttaatatt ggtaattttg aatggtttaa
    61381 aaccaatcca catcatttga tcataaattt ctttttcaat ttggatgaac acatccgcaa
    61441 taacgccata tgctttcgcc caagcttgaa tgacagggtc attttcttct aatcctgtca
    61501 cgtcttgaat ggcttttaat aaattttccc ccacaattgg ataatgttca gcataaactt
    61561 gtagtgcgca gtgtttatat gcaactggca taataactgg tttaataaca cttaaattat
    61621 cgatattaac cgctgcggcc attacagctt gtgctaatgc tgaagattgc atgcctcttt
    61681 tttggttcgt ttgattaaac atatttaaaa gttcaggatg tgccttaaac atttttggat
    61741 aaaagattga cgtaatttct gttccttttt ctttaagcaa tagcaccgtt tgtttgataa
    61801 tgtctttctc ttgttctgta agcatgatac tcctccttta atctgtctat tttgattatt
    61861 ctactaaaaa ttccgatatt caattaattg gtttgagaaa atataaataa aatggcaaat
    61921 ttgataattg tatgacattt ttaatttttt aaatacttat caataagcat tgtgtacaat
    61981 tgtctgtttg tacaccgacg attgcgcgca tttatttgac taattcaaaa aacattgttg
    62041 ttttcttaga aaaaagtaaa catgataata aaaatgtgaa agtgtaaata atcactggcg
    62101 aagtacgaag actaaagaca tctaagatgt aatcgtatac aaattaaaaa ggtgtaaaaa
    62161 ttaaaataaa atgtgaaata aatcacaatt taatattgac ccagtactta atgcatgtta
    62221 cattttatat gtgaaataaa tcacaaactt aaaagcggat gacacatgac cttttaagtt
    62281 atgcgttgaa aataaaagag atgtttattt gcttttgtat cgtcaataag cagcattaaa
    62341 ctaacatatt tgaaactaca cgtatgttag tgaattaatc ataagggagt ttttgtaatg
    62401 aacaaattta aagggaacaa agttgtatta ataggtaatg gtgcagtagg ttcaagctac
    62461 gcattttcat tagtgaacca aagcattgtt gatgaattag tcatcattga tttagacgct
    62521 gaaaaagttc gaggagatgt tatggattta aaacatgcca caccatattc tccaacaaca
    62581 gttcgtgtga aagctggcga atacagtgat tgtcatgatg cggatctagt tgtcatctgt
    62641 gctggtgctg cacaaaaacc tggagaaaca cgtttagatt tagtatctaa aaacttgaaa
    62701 atattcaaat caattgttgg tgaagtaatg gcatcaaaat ttgatggtat tttcttggta
    62761 gctacaaatc ctgttgatat tttagcgtat gcaacatgga aattctctgg tttacctaaa
    62821 gaacgtgtta taggttctgg tacaatttta gactctgcac gctttagatt attgttaagc
    62881 gaagcgttcg atgttgcgcc acgtagcgtc gatgctcaaa ttattggtga acatggtgac
    62941 actgaattac cagtatggtc acacgctaat attgcgggtc aacctttgaa gacattactt
    63001 gaacaacgtc ctgagggcaa agcgcaaatt gaacaaattt ttgttcaaac acgtgatgca
    63061 gcatatgaca ttattcaagc taaaggtgcc acttattatg gtgttgcaat gggattagct
    63121 agaattactg aagcgatttt cagaaatgaa gatgctgtat tgactgtatc agcattatta
    63181 gaaggcgaat atgatgaaga agatgtttat attggtgttc cagcagtcat caatagaaac
    63241 ggtattcgca acgtcgtaga aatcccatta aacgacgaag aacaaagcaa gttcgcacat
    63301 tcagctaaaa cattaaaaga tattatggct gaagcagaag aacttaaata actttttata
    63361 aaatctatac cattccaaaa attgtaaaac cttaccccaa aaaattgtat aaagggctat
    63421 ttgatacaac tatacgtgtc gagtagccct atttttaatg tagtgaaagt cgttgttgaa
    63481 attaaattta aatctggcac ttaagcttta atcatagcat taagatgatt gtctagcaga
    63541 ggcgatttgc ggactcacta cagtgcatga tgaacttaat gtttcaaatg caacattaaa
    63601 aataaaagca gcgatgtcac ttcttactcc gtacatcgtt gccattagtt ctgttgatat
    63661 ttcggttagt cttaatcccc gagcaattct tcaatttcat ttttgataac tgtaacgtga
    63721 ggaccgtaaa ttacttgcac accagtgcct tgctggatta cacctttggc accagtactt
    63781 tcgagtagta ctttatcgac tttgtcattt tgatgaagtg taacgcgtag tctcgttgca
    63841 caacagtcaa cgatttcaat gttatccttg cctcccaaac cagcaacaat agtttgtgct
    63901 ctttcagtag cctcaacttg ttgtgctgca gctttatctt cacgaccagg tgttttgaaa
    63961 ttaaatttcg taattaagaa tctgaaaacg atgtaataca aacagaacca cacaattcca
    64021 ataggtatga catataggta gtttgtttta ctattacctt gtagcacacc aaaaagtaag
    64081 aaatcgataa agcctccact gaaggtttga ccaattgtaa tgttgaaaat gtctgccatc
    64141 ataaatgcta atccatcaaa gaaggcatgg attacataaa gaataggtgc gacaaacaag
    64201 aaactaaact ctaaaggttc ggtaatacct gttaaaaatg aagtgagtgc agcggataac
    64261 attaaaccgc cgacaacttt tttatgttca ggtttagccg tgtgataaat tgcaagtgcg
    64321 gcaccacata agccgaacat catcgtaata aaacggcctg acataaagcg tgacacacct
    64381 gaataatact tcgtcacatc tggatcacca agttgagcaa agaagatgtt ctgcgtacct
    64441 tgaactaagt gacctttgac ttctaaagta ccaccaagtg ccgtctgcca aaatggtaag
    64501 taaaaaatat gatgtaaacc gagtggacct aacaatctta agatgaagcc ataaacaaaa
    64561 gtaccgatgg cacctgtttt cgttacaaat ccacccacat gataaatgcc ggcttgtatg
    64621 cttggccaaa tgaaaaacat caatacacct aaaaagattg cggcaaatgc tgtgacaata
    64681 gggacaaatc tagagccacc aaagaaacct aaatacggtg gtaataccac tttgtgatat
    64741 ttgttgtgaa gtattgcggt cataatacct gtgataatcc cgccaaaaac accggtttca
    64801 accgtttgta taccgagcac catgccttgt ccattttgtg caagctgatc ttttgccaat
    64861 gtgcccgtga tagttaataa gccattcata gttgcgttca taattaagaa accgagcagt
    64921 gcagctaaac ctgcagtacc tttatcgctt ctagataatc cgattgcgac accaattgca
    64981 aagatgactg gtaaattttg gaaaacaata ctacctgcag ctgacattaa tgtaaaaata
    65041 ttttgtaata aggtaatatc taaaataggg tatgctttaa cggtgtttgg attacttaat
    65101 gcaccaccga tacccaacaa tagacctgca gctggtaaga ttgcgatagg tagcataaag
    65161 gacttgccga actgctgtgc tttttcaaat aaagatttca tcaacatccc tcctaattat
    65221 tctcaatata gcttttgaga aatttaatat caatatatat tctgtgtatg aaaatatttt
    65281 tcataaaaat tgtttgaatc atgtaacaat catataaatt gctgtttata ttgtggtgaa
    65341 aatgagttga caaaagtcgg tatagttctg tagacatcct attttttagc gaggtggggt
    65401 gacaaggcat atcggataaa tttataagcc ataaaataga taaatagtgt ttgtatccaa
    65461 aactatgtga aagttaaaac tatttttcaa aataaacatg attcaccaca taaataaata
    65521 tacataagct atcgataaca acttaagaaa ggtggatata tataatgaaa agaaagatta
    65581 ttatggattg tgatccagga cacgatgatg caatagcatt aattttagcg ggggcaattg
    65641 acagtccact agagatatta gctgtaacca cagtcgcagg taatcaatca gttgacaaga
    65701 acacgacaaa cgccttgaac gtattagata ttatgggacg ccaagatata gtagtagcga
    65761 aaggtgcgga taggccgtta attaaactag ttgcctttgc ttctgaaata catggggaat
    65821 ctggattaga tggtccgaaa ctaccgtcga caccatcacg tcaagcagtt gcaatgccag
    65881 catcagatgt gattattaac aaagtgatga cgagtgatac acctgtaaca attgtagcga
    65941 caggtcctct tacgaatgta gcaacggcat tgattcgtga gccaagaatc gctgagcata
    66001 ttgaatctat tactttgatg ggtggtggta catttggaaa ttggacgcct acagcagaat
    66061 tcaatatttg ggtagatgct gaagcagcga agcgtgtttt tgaaagtggg attactataa
    66121 atgtgtttgg tttagatgta acacatcaag ttttagccga tgaacacgtg attgaacgct
    66181 ttgaaagtat caataatcct gttgcacagt tcgtcgtaga attattgcaa ttctttaaga
    66241 agacatacaa gactcacttt aatatggacg gtggtccaat acatgatgct tgtacaattt
    66301 tgtatttgtt acaaccagaa ttgtttacaa tggtacccgt taatatcgac attgaacatc
    66361 aaagtccact aacttatggc actatggctg tagatttaaa tcatgttaca ggtaagcctg
    66421 ccaatgccta ttttgctaca gcagttgatg ttgaagaagt gtggaacttg atagatcata
    66481 agttacgtac atacgaataa taatacttaa ttaaatagat acagttaacc ctaaggtgcc
    66541 tgatttaagc gtccttaggg tttttgtatt ggattacaat ggtcatggca atttgataat
    66601 gattgagata tgtatgttaa aaatgcttca aaaaacacaa aatacaaaaa tatatgcaaa
    66661 aatttcaatt gctttatttt aaaagattaa aatcacaaaa aataccgata taattggaat
    66721 gttgtcgaaa catcacaatg atgtacatac tcattttgaa aatatgtgtt caacgagtgt
    66781 acatcggtgt gacgattgat gataaattga gctcagatgc tcaaaggagg atcgaacatg
    66841 aacggggata atcagcaaat actcagagaa attgtattga atcctactat tcatggtaaa
    66901 gaacttgaat cgatatttgg tttgtctcgt agacaactag gatatcgcat tcaaaaaatc
    66961 aatttgtggc ttgaacaaga gggttatcca aaacttgaaa gaacaagcca aggaaatttt
    67021 attgtaagtt ctgaaatcat gacgttattc aaacgagatg tatcggagca gcaaatgtta
    67081 aacggcaaca atgtcatttt tagcatagaa acacgtcgtt attatttaat gctcatgctt
    67141 tttagtaagg aaaacgcaat gtctctaaac catttttcaa ttgatttaca agtcagtaaa
    67201 aatactgtca ttcacgatat aaatcatgtg aaagagcaat tggaagatca tggtttgtca
    67261 cttaagtatt ctcgaaaaca tggttatgaa attgttggtg atgaatttga agttcgccgt
    67321 ttcttcatta agttgattga tcaaaggtta aatcatgata ttactaaaag tgaagtttta
    67381 aaggcgctca acttaacatt cgaagatatc gcatatcaaa aagacaagat caaacaggta
    67441 gaacaatttt tgaagagtcg ctttatagac aaatcactga gttcattgcc ttacgtcctt
    67501 tgtgtgattc gtagacgaat tcaaagtggt catgtgatta atccattaaa tattaattat
    67561 cagtatttga gggatacgaa agaatatcaa gcaacggaga ttatgacgca acatgagccg
    67621 gatttgccag aagcggaaaa gttatatttg acgctacact tactttcaac aagtgtgcaa
    67681 tggactgatt tgcaggaatc agataacata tcgaatttaa cgatggccat tgatcaaatg
    67741 attcaccatt ttgaacaaat tacttttatt aacattgaag ataaggagaa attatcacag
    67801 caactcttgt tacatttaac gcctgctttt tataggatta aatataactt aacggatcgt
    67861 gatgaattaa taaatccttt acaaggaaat tatcaatcct tatttcatat ggtgaaacaa
    67921 tcatgtcaat cgttaactga atatttcgga aaatcattgc ctgataatga aatagcatat
    67981 ttaaccatgt tgtttggagg tagtttgaga cgtcaagatg aaaacttcga tggcaagata
    68041 aaagctatta tcgtgtgtac acaaggtacg tcagtatcac aaatgatgtt atacgagttg
    68101 cgaaacttat ttccagaaat tattttctta gatgcgattt cacttagaac atttgaaaat
    68161 tacacattag attatgacat cgtcttttca ccaatgtttg tcttaacaca taaaaaatta
    68221 tttatcacaa aagtagcttt atctgaaaat gagcaatgaa agttacgtaa agaagtgatg
    68281 aagtacatta ataaggaatc ggctgacatt gataaggaaa taaacaagtt aatggcatta
    68341 attgaacgca ctacgacagt taatgacatt acagaactac gtgatggttt agaagatttt
    68401 attgcgaatt ataattcaat ttcaaccatt aatggatcga ttgtcacaca aaataagaca
    68461 ttagatttag ctgacttgat accagcaagg cacgtgaaaa gaatacatca tgttgaaaat
    68521 attgatgaag ctattgctaa agcaagtgat gtgttagttg ctaatcattt tattgatatt
    68581 aaatatattc atgagatgca acaggcattt gatgattcgt atatggttat catgcaaaat
    68641 attgctattc cacatgcata ctctgaaaag catgtacata aaacagcgat gagtatgttg
    68701 atattacaag aaccaatata catgtcagat ggcacagcaa tccatattat tgtacctatt
    68761 gctgctgttg ataaagtgac acacttaaga gcgttactac aattgagaga tgtggcgcaa
    68821 aacaatgacg caattaagcg catcatacaa agtcgcaaaa attctgatgt aaatgagatt
    68881 ttaaaaaatt attcaaataa agaagcgagg gaaaatggat gggacagcaa ttagtgcata
    68941 aagaaaatat aatgctcaat ttgtcggcaa ctgataaaga atctgtattg tcacaaatgt
    69001 cagatgtgtt atttcaaaat gggttcgtaa agtcaacgtt taaagatgca gtcatcgaca
    69061 gagaaaaaga gtttgctact ggtttaccaa cgcatctatg ttcggttgct ataccgcata
    69121 cagatgtcga acatattaac catagaacga taggtgtggc tgttctagaa aaagaagtgc
    69181 cgtttattga aatgggaaca cttgatcaac agacagaagt gaaaatcgtt tttatgttag
    69241 caatggataa agtagatgat caacttaagc tgttacaaca gttgatgcaa atttttcaaa
    69301 gtgaagaaaa attggagcag attctacgaa cgaaagatga aacaatttta gcaacactaa
    69361 tcaatgatta tttgaaatat aactaaaaat taattggagg aattgaaaat gaaacaagta
    69421 ttagtagcgt gtggtgcagg tattgcaacg tcaacagtag taaataatgc aattgaggaa
    69481 atggcaaagg aacacaatat taaagtagat attaaacaaa tcaaaattac agaagttgga
    69541 ccttatgaag acactgcaga tttattagtt acaactgcaa tgacaaagaa agaatataaa
    69601 ttcccagtta tcaacgcacg taatttctta actggtattg gtattgaaga aacaaaacaa
    69661 caaatcttaa cagagttaca aaaataactg aattgatatg taacgtgggt caataacata
    69721 taaatatatg caaattgcat ataaatgact atcacgacaa atgattgtag atgacataga
    69781 aacgcagtga ctgtaattca aagggattgc agtcgaatgt agcaagggtt gtcgttgaaa
    69841 tgactgtaac gtcaaatgta atcacaatcg gaaagtatcg tgacaatgca tatataacag
    69901 ggagggttta aatatgagtt acttcactga ttttgtaagg ggatttttag atttaggtgc
    69961 aactgttatt ttaccggttg tcatattctt gcttggccta ttctttaggc agaaaattgg
    70021 agcggcattt aggtctggtt taacaatagg tgtggctttt gtagggattt tcttagtcat
    70081 cgatttatta gttaaaaatt tagggccagc agcacaagcg atggttaaaa atttaggcgt
    70141 cagtctgaat gtgattgatg taggttggcc agcaacatca tctatcgcgt gggcatcatc
    70201 tgtcgcagca tttattattc cactcggaat cattgttaac gttgtattgc tagtaactaa
    70261 agtgacaaag acgatgaatg tagatatttg gaatttttgg cattatacgt ttacagcagc
    70321 aatggtttat gccgtatcag gcagtatttg gcaagcgtta ttagcagcag ttattttcca
    70381 agttatctgt ttgaaagtag cagattggac agcaccgatg atgagtgagt tctttgattt
    70441 accaggtgta tcgattgcta caggaagtac aatttcttat gcaccaggta tttacttagt
    70501 taaattgtta caaagagtac ccggtctgaa taagttagat gctgatcctg aaacaattca
    70561 aaaacgtttt ggcgcatttg gagagtctat ctttgtcgga ttaattttag gtttaggtat
    70621 tggtgtgtta gcaggttaca aacctggaga catcattaat ttaggaatgt caatggctgc
    70681 agtaatggta ttaatgccta gaatggtaaa aatcttaatg gaaggtttaa tgccagtttc
    70741 agagtctgca agaacatggc taaataaacg ttttggcgaa cgtgaaattt atattggatt
    70801 ggatgcggct gtagcattag gtcatccagc ggttatttcg acagcattaa ttttagtacc
    70861 tatcactgtt ttattagccg ttattttacc aggaaaccaa gtattacctt ttggtgactt
    70921 agcaactata ccatttgttg tcgcgtttat tgttggtgca gcaagaggaa acattattca
    70981 ttctgtcatt gtgggtacga ttatgattgc aatttcacta tatattgcaa cagacgtagc
    71041 acctattttc acagatatgg cgaaaggtac gaatgtacaa atgccaaaag gttcatctga
    71101 aatttcaagt attgatcaag gtggtaatat cgttaactat cttatcttta aactatttag
    71161 tctattcaat taaaaatcga ggtgtttgtg gtgaaagctt tagtaaaaac aagagaagga
    71221 catggcaact tagaacttct tgataaagaa gttgcaacac cgctagatga taaagtaaag
    71281 attaaagtac attatgcagg aatttgtggc acagatattc atacttatga aggtcattat
    71341 aaagttaatt ttccagtgac attaggtcat gaattttctg gtgaaatcgt tgaagttgga
    71401 gcagacgtta aagattttaa agttggtgac cgtgtcactt ctgaaacgac attctatgtt
    71461 tgtaatgagt gtgaatactg tgaatcaaaa gactataatt tatgcaacca tcgaaaaggt
    71521 attggaacac aagttgatgg cgcatttact aattatgtca ttgcacgtga agaaagtttg
    71581 catcatattc cagacgaagt gtcgtatcag tctgcagcta tgacagaacc attagcatgt
    71641 gcacatcatg gcgtttctaa gattcaagtc aattcaggcg atgtagcagt tgtaatggga
    71701 cctgggccaa tcggattact tgtagcacaa gtgttgaaaa gtaaaggcgc aactgttgtg
    71761 gtaactggat tggacaatga caaagtcaga ttagataaag cagaagcatt gcacatggat
    71821 tatgtagtca atttacaaca aacagactta aaaacgtata tcaatggaat tacagacgga
    71881 tacggtgcag atgttgttgt tgaatgttca ggtgcagttc cagcagcacg acaaggtttg
    71941 gatattttac gcaaaaaagg tttctacagt caaataggta tttttaagga tgctgaaatt
    72001 acatttgata tggaaaaagt gattcaaaaa gaaataacag ttgttggtag tagaagtcaa
    72061 aagccggcag attgggaacc ttcattgcaa cttatggcgg atggtttagt aaatgctgaa
    72121 gctttggtga caaaaatata tgatatttcg aaatgggacg aggcgtatca acatttaaaa
    72181 tctggcgaag gtattaaagc attacttaag ccgctcgatt tagatgaaaa tgaaggagag
    72241 aattaatatg gtagaatcaa tgctaacttt tatgcttggg ccattgagac aaatcactga
    72301 tttttatatg gaacatctac ttgtaagtaa ttctatcgtc attgcaggtt attttgcgac
    72361 aggtattttt aaaaagaaaa aagttgtgaa ttaaatcaaa gttgaggtga tttacaagtg
    72421 aaagctttga aattatatgg cgtggaagat ttacggtatg aggataatga aaagccagtc
    72481 attgaaagtg cgaatgacgt tattgttaaa gtacgagcga ctggaatatg tggttcagac
    72541 acgtcacgat acaaaaaaat ggggccatac attcaaggta tgccatttgg tcatgaattt
    72601 tcaggtgttg tagatgccat tggaagtgat gttacgcatg ttaatgtagg cgacaaagtg
    72661 acaggttgcc cagccatacc ttgttatcaa tgcgagtatt gtttaaaagg tgaatatgca
    72721 cgatgtgaaa agttgttcgt cattggctca tatgaacctg gatcgttcgc ggaatatgtc
    72781 aaattgccag cgcaaaatgt tttaaaggta ccagacaatg ttgattacat tgaagcagca
    72841 atggttgagc catcagccgt tgttgcgcat gggttttata aatcgaatat acaacctggt
    72901 atgactgttg cagtaatggg gtgtggcagt ataggattgt tagctattca atgggcacga
    72961 atatttggtg ctgcgcatat catcgctata gatatagatg cgcataaact agatattgct
    73021 acatcattgg gcgcacatca aaccatcaat tcaaaagaag aaaatcttga gaaattcatc
    73081 gaaaatcatt acgccaatca aatcgatata gctatagaat catcaggtgc taaagttacg
    73141 attggtcaaa tattgacgct acctaaaaaa ggtggcgagg tggtattact cggaatacca
    73201 tatgatgata ttgagattga tcgcgttcat tttgaaaaaa ttctgcgtaa cgagttgaca
    73261 gtatgtggct cttggaactg tttgtccagt aattttccgg ggaaagagtg gacggcaacc
    73321 ttacattata tgaagacgaa agatattaat gtaaagccta ttatttctca ttttttaccg
    73381 ttagaaaaag gcccggagac atttgataaa ttagttaaca agaaagaacg atttgataaa
    73441 gtcatgttta cgatttatta gtatgcacct ttgaggacga aaacgctggt atagttatag
    73501 ctatgaaagt gcgaatgccg tctggtctac agatactatc gaaataattc atcttcgaat
    73561 atacgttgct aaatagccgg tttacttgtg tgaaatatgc ttgtgaatcg gttgttttgc
    73621 attttgtata cttaaaatga gatggcaata tttgataatt tttaaagtga aaatcaagta
    73681 cagccactta ataagataaa tttattataa tatatggtaa aatgatggca gtaataatga
    73741 atttgaaaaa gagtaaacat taataccttt aacaatttaa tatcgtcagt gttaatgatt
    73801 aactgcatgg caaaacaact tagaatggtc agttacaaaa atacattttt ataaaaatta
    73861 tcacactatt gtgacaactg cctttggctt aataaaagag gaaagtgagc aataggttgg
    73921 gcttatgtgc gggcataggt cagtaatgta taaatggaaa tgatgtaatg acagaatgga
    73981 ggacaacatg atttatgcag gtattttagc aggaggtatt ggttcgagaa tgggaaacgt
    74041 gccattacca aaacaatttt tagatattga taataaaccg attttaattc atacaattga
    74101 gaagttcatt ttagtgagtg aatttaatga gattattatc gcaacgccag cacagtggat
    74161 ttcgcatacg caagatattt taaaaaaata taacattaca gatcaacgtg tcaaagtagt
    74221 tgcaggtggt acggatcgaa acgaaacaat tatgaacatt atcgaccata ttcgcaatac
    74281 gcagggtatc aacgatgatg atgtgattgt aactcatgat gccgtaagac catttttaac
    74341 tcaacgtatt attaaagaga acattgaagt agcagcaaaa tatggtgcag tagatacagt
    74401 cattgaagca attgatacga ttgtaatgtc taaagataaa cagaacatac acagtatccc
    74461 tgtaaggaat gaaatgtatc aaggccaaac accacaatca tttaatatta aattattaca
    74521 agatagttat cgtgccttaa gtagtgcaca aaaagaaatc ttatcagatg catgtaaaat
    74581 cattgtcgaa tctggacatg cagttaaatt ggtacgcgga gaactataca acattaaagt
    74641 gacaacaccg tatgatttaa aagtagcaaa tgccattatt caaggtgata ttgccgatga
    74701 ttaatcaagt atatcaactc gttgcaccga gacagttcga cgtcacatat aataatgttg
    74761 atatttatgg taatcatgtc atcgtaagac ctttatactt gtctatttgt gcagctgatc
    74821 aaaggtatta cacaggtcga agagatgaaa atgtactgcg caaaaaatta ccaatgtcat
    74881 tagttcatga agctgttggt gaagttgtat tcgatagtaa aggcgtattt gaaaaaggta
    74941 cgaaagtagt aatggtgccg aatacaccta cagaaaaaca cgacgtgatt gcggagaatt
    75001 acttagcctc tagttatttt agatctagtg gttatgatgg ttttatgcaa gactacgttg
    75061 tgatggcaca tgatcgtatc gttccgctgc ctaatgacat tgatttgagt acgatttcat
    75121 acacagagtt agtgtcagta agttatcatg ctatacaacg atttgaacgt aaatctatac
    75181 ctttgaaaac cagctttggt atttggggtg atggtaactt aggttatatt actgctattt
    75241 tgctgcgtaa gttgtaccca gaagctaaaa tttatgtatt tggtaagaca gactataaat
    75301 taagtcattt ttcatttgta gatgacatct ttacagtaaa tcaaatacca gatgatctta
    75361 aaattgatca tgcatttgaa tgtgttggag gtaaaggaag tcaagttgca cttcaacaaa
    75421 tagttgaaca tatttcacca gaaggcagta ttgctttgtt aggcgtaagt gaattacccg
    75481 tggaagtgaa tacacgatta gtacttgaaa aaggattaac gttgattggt agtagtcgaa
    75541 gcggctctaa agattttgag caagttgttg atttatatcg taagtaccca gacattgttg
    75601 aaaagttagc cttattaaaa ggacatgaaa ttaatgtatg tacgatgcaa gatatcgtcc
    75661 aagcgtttga aatggattta tcgacatctt ggggaaaaac agtattgaaa tggacgattt
    75721 aataaaccga tgggggaata acaatgacaa aaacgaaaca agcaatacat attgataaca
    75781 tatactggga acgtgttcag ttatatattg aaggacatag tgaaggcgta gatttaacat
    75841 caggacaatt tgttctgagg aatttaaccg aaacaaaaac attagaagca aatgaaatga
    75901 aaatagacgg taatacattt ctatgtagat tcaatgtcgc aatattagac gatgggtatt
    75961 atttaccaat ggataaatat ttatttgttt atcatgacca gttagagtat attggacaac
    76021 ttaatccaaa tattattgat caagcttatg cggcattaaa tgaagagcaa attgaagaat
    76081 acaatgagct gactacacaa aatggaaaag caaactatgt gttagccaac gaagctaaag
    76141 ttttccgcaa aggtggcgta tcacaacata cggtttatac cattactcca gaaatagcaa
    76201 gtgacgttaa cgaatttgta tttgatattg acatcacctt acctcaagag aaatcagggg
    76261 tcattgcgac aagcgcacac tggcttcata aacaaggcca taaagcttca tttgaaagta
    76321 gaagtttctt atttaaagct atttttaata ttacaaagtt actacatatt aaaagaagca
    76381 aaacaatatt attcacatca gattcacgtc cgaatttatc aggtaacttt aaatatgtat
    76441 atgatgagtt actacgccaa aaagtagatt ttgattatga tattaaaacg gtatttaagg
    76501 cgaatattac ggatagacgt aaatggagag acaagtttag attgccatat ttacttggta
    76561 aggctgatta tatttttgtc gatgatttcc atccattaat ttatacggtt cgctttagac
    76621 catcacaaga aattattcaa gtgtggcatg ccgttggtgc ttttaaaaca gttggcttta
    76681 gtcgtacagg taaaaaaggt ggtccgttta ttgattcatt aaatcatcgt agctatacaa
    76741 aagcttatgt atcatcagaa accgatattc cattttatgc tgaagcattt ggaattagag
    76801 aagaaaatgt tgtaccaaca ggtgtaccac gtactgatgt actatttgat gaagcttatg
    76861 caacacaaat taaacaagag atggaaaatg aattaccact tataaaaggt aagaaagtta
    76921 ttctattcgc accgacattt agaggtaatg gtcacggtac ggcacattat ccatttttta
    76981 aaattgattt tgaacgttta gcaagatatt gcgagaagca taatgcagtt gtgttattca
    77041 aaatgcatcc gttcgtaaaa aatagactca atatttcacg tgaacataga caatacttta
    77101 tcgatgtgtc agattatcgt gaagttaacg atattctctt tgttacagac ttgttgatta
    77161 gtgattattc atctttaata tatgaatatg cagtatttaa aaaaccaatg attttctatg
    77221 catttgactt agaagattat attacgacgc gtgatttcta tgaaccatat gaatcatttg
    77281 ttccaggtaa aattgtacaa tcctttgatg cattaatgga tgctttggac aatgaagatt
    77341 atgaggttga aaaagttgta ccattcttag ataaacattt taaatatcaa gatggtcgct
    77401 caagtgaacg tttagtcaaa gatttgttta gacgctaatg ttggcatata ttacttgcca
    77461 tgcaaatgat cataaggcat agttttcaag aagggttatg acaatgaagt gagcaacatg
    77521 tcatgatgag aagcaggact atacaatgag aataaccttt tgatttttca tgcataagag
    77581 gctgggacat aaatccctaa aaaaacagca gtaagataat tttcaattag aaaatatctt
    77641 actgctgttc tctatttata caatacttcg tattgaatgg cttcgctttg cccgtctggc
    77701 acattactgt aaaattctat aaatagaatt tttgatgacg ggtcccttcc tagggtgccg
    77761 tctcagccac cccaaccggc acattgttgt aagctgacta tatgtcagct tctgtg
//
LOCUS       DAKSQE010000002        54300 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.002, whole genome shotgun sequence.
ACCESSION   DAKSQE010000002 DAKSQE010000000
VERSION     DAKSQE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 54300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54300
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.002"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..356)
                     /gene="cap8H"
                     /locus_tag="O0V33_000064"
     CDS             complement(<1..356)
                     /gene="cap8H"
                     /locus_tag="O0V33_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8H"
                     /protein_id="HCX8974765.1"
                     /translation="MRIAILGATNIKHMSLLSHYLNHIDLNINEVDIIYTDKYDIEEN
                     IQGINNYYKYKVDIKEDWTFIKKAIAYYRFRPYAMKILKENHYDFVIVWGSYTGHLFK
                     SFLEKHYKNKFILNIR"
     gene            complement(359..1483)
                     /gene="cap8G"
                     /locus_tag="O0V33_000065"
     CDS             complement(359..1483)
                     /gene="cap8G"
                     /locus_tag="O0V33_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HCX8974766.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGF
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKIPVIYSTHPRSWKKIEESKFEFDPLVKQLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIIGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            complement(1487..2596)
                     /gene="cap8F"
                     /locus_tag="O0V33_000066"
     CDS             complement(1487..2596)
                     /gene="cap8F"
                     /locus_tag="O0V33_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HCX8974767.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFVVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLREYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNNRNVELTLNYVDDIVAEIKRAIEGNPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDNEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            complement(2609..3637)
                     /gene="cap8E"
                     /locus_tag="O0V33_000067"
     CDS             complement(2609..3637)
                     /gene="cap8E"
                     /locus_tag="O0V33_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HCX8974768.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKRFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVDYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            complement(3627..5450)
                     /gene="cap8D"
                     /locus_tag="O0V33_000068"
     CDS             complement(3627..5450)
                     /gene="cap8D"
                     /locus_tag="O0V33_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
                     /protein_id="HCX8974769.1"
                     /translation="MAHLSVKLRLLILALIDSLIVTFSVFVSYYILEPYFKTYSVKLL
                     ILAAISLFISHHISAFIFNMYHRAWEYASVSELILIVKAVTTSIVITMVFVTIVTGNR
                     PFFRLYLITWMMHLILIGGSRLFWRIYRKYLGGKSFNKKPTLVVGAGQAGSMLIRQML
                     KSDEMKLEPVLAVDDDEHKRNITITEGVKVQGKIADIPELVRKYKIKKIIIAIPTIGQ
                     ERLKEINNICHMDGVELLKMPNIEDVMSGELEVNQLKKVEVEDLLGRDPVELDMDMIS
                     NELTNKTILVTGAGGSIGSEICRQVCNFYPERIILLGHGENSIYLINRELRNRFGKNV
                     DIVPIIADVQNRARMFEIMEMYKPYAVYHAAAHKHVPLMEDNPEEAVRNNILGTKNTA
                     EAAKNAEVKKFVMISTDKAVNPPNVMGASKRIAEMIIQSLNDETHRTNFVAVRFGNVL
                     GSRGSVIPLFKSQIEEGGPVTVTHPEMTRYFMTIPEASRLVLQAGALAEGGEVFVLDM
                     GEPVKIVDLARNLIKLSGKKEDDIRITFTGIRPGEKMYEELMNKDEIHPEQVFEKIYR
                     GKVQHMKCNEVEAIIQDIVNDFSKEKIINYANGKKGDNYVR"
     gene            complement(5470..6234)
                     /gene="cap8C"
                     /locus_tag="O0V33_000069"
     CDS             complement(5470..6234)
                     /gene="cap8C"
                     /locus_tag="O0V33_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HCX8974770.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMLDLLRQATTQGVTEVIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVITGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGKKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            complement(6237..6923)
                     /gene="cap8B"
                     /locus_tag="O0V33_000070"
     CDS             complement(6237..6923)
                     /gene="cap8B"
                     /locus_tag="O0V33_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HCX8974771.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKSLIIDGDMRKPTQNYIFNEQNNNGLSNLII
                     GRTTMSEAITTTEIDNLDLLTAGPVPPNPTELIGSERFKELVNLFNERYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKTLMEKAGSNILGVILNKAKVDKS
                     SSYYHYYGDE"
     gene            complement(6939..7607)
                     /gene="capA"
                     /locus_tag="O0V33_000071"
     CDS             complement(6939..7607)
                     /gene="capA"
                     /locus_tag="O0V33_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HCX8974772.1"
                     /translation="MESTLELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKY
                     QANTQILVNQTKGDNPQFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSK
                     LSSMLTITNQENTQLINIQVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKA
                     DGTAVKVAPKTVVNLIGALFLGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSI
                     QKFN"
     gene            complement(7952..10561)
                     /gene="adhE"
                     /locus_tag="O0V33_000072"
                     /gene_synonym="adhC"
     CDS             complement(7952..10561)
                     /gene="adhE"
                     /locus_tag="O0V33_000072"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCX8974773.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEIN
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTSIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQRTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
     gene            complement(10947..12122)
                     /locus_tag="O0V33_000073"
     CDS             complement(10947..12122)
                     /locus_tag="O0V33_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCX8974774.1"
                     /translation="MFIGKNLEYVRKLNALSRKELSEKINVSEQAIWQYETKNMMPEI
                     NKIYDMTSIFNVKSSYFISEQPEELLINSVDKHSIAFRAKNYKVSTKLLNKQYYQAMY
                     LSNLTSYLFSFVKIPDNIILSLINNLDDLLNGNLESLNKKESIKETAKVVRAKILQDE
                     SNEALLFMLEKAGIVIYEKRINDSIDAYSFWSKDQTPFIILGTNKGVAVRRNFDLAHE
                     LGHLVLHRHIQFDLLSPEEYKTIEHEADIFASEFLLPEEAFKKDFDQMTKKSNPDYLA
                     VLKEKWYVSIQAIAMRAYYLGLMSSTQYRYFWASLNKKGYKSKEPLDDVIEMSRPVKM
                     NSLLKLYFDRNILTPQKLLNYLKVDETFLNHLAGINLKLFKDYVNENREYNITNLYK"
     gene            complement(12128..12895)
                     /locus_tag="O0V33_000074"
     CDS             complement(12128..12895)
                     /locus_tag="O0V33_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8974775.1"
                     /translation="METIKQEVRESIVKGIFAGYKDYVNERNEVKKRMVISDAYVYTK
                     SNHIENQVAKHLENFVKYIKENAGPSWKYLKFIFNKDEEKNNIMFILKNEDYFDEKNV
                     SVGKSLVADKNQKSKNYLEGLIAKNSGINFDTVGEDFKIRHQMTADSILFNIKEGSNR
                     DINSYFLIITYRIDKESKQLAAIKQWLPNPETNSAIMVDDLTPLLDKVIVEREDYHID
                     EEELEVLKNDGELEFIDVEHAFGISIDEDNDMIESER"
     gene            complement(13052..14587)
                     /locus_tag="O0V33_000075"
     CDS             complement(13052..14587)
                     /locus_tag="O0V33_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCX8974776.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVQYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFATLQQHLPEMKRYADIIVVCYHGGFEKDIESGTPTEVLTGENEGYGMLE
                     AFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGKVVLSTDEYENVSVESCELL
                     PVIDDPTFTIDEDDQHIRKQLEDWLDYEITTLPYDMMINHAFEARVAPHPFTNFMNYA
                     LLEKSGADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGFKLKQAIERSA
                     EYFDVKNDEVSVSADFLEPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMYIDAPVVKDIQVEGAQLLIDFLSNNKLMRIPQVVDFK
                     VEK"
     gene            complement(14638..16182)
                     /locus_tag="O0V33_000076"
     CDS             complement(14638..16182)
                     /locus_tag="O0V33_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCX8974777.1"
                     /translation="MKNIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQQTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVNYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITAWMSKDNDGKSL
                     KASGLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADP
                     QPNTPDDHTPPNQTEKVSFDIPQNVSVNEPFEVTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNATIKVNAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
     gene            16411..17367
                     /locus_tag="O0V33_000077"
     CDS             16411..17367
                     /locus_tag="O0V33_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCX8974778.1"
                     /translation="MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            17581..18354
                     /gene="phnC"
                     /locus_tag="O0V33_000078"
     CDS             17581..18354
                     /gene="phnC"
                     /locus_tag="O0V33_000078"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCX8974779.1"
                     /translation="MSQIKFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSKIYGRTIKEDEKLGVN"
     gene            18356..19156
                     /gene="phnE"
                     /locus_tag="O0V33_000079"
     CDS             18356..19156
                     /gene="phnE"
                     /locus_tag="O0V33_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCX8974780.1"
                     /translation="MPLETPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            19153..19968
                     /gene="phnE"
                     /locus_tag="O0V33_000080"
     CDS             19153..19968
                     /gene="phnE"
                     /locus_tag="O0V33_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCX8974781.1"
                     /translation="MKQEIEKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIESLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIL"
     gene            complement(20099..21277)
                     /gene="deoB"
                     /locus_tag="O0V33_000081"
     CDS             complement(20099..21277)
                     /gene="deoB"
                     /locus_tag="O0V33_000081"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCX8974782.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
     gene            complement(21305..21967)
                     /gene="deoC"
                     /locus_tag="O0V33_000082"
     CDS             complement(21305..21967)
                     /gene="deoC"
                     /locus_tag="O0V33_000082"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCX8974783.1"
                     /translation="MKFEKYIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKVFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVVKAAEGHTVKVIIETVLLDHDEIVKASELTKAAGADFVKTSTGFAGGG
                     ATAEDVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADS
                     DY"
     gene            complement(22048..23400)
                     /gene="tet(38)"
                     /locus_tag="O0V33_000083"
     CDS             complement(22048..23400)
                     /gene="tet(38)"
                     /locus_tag="O0V33_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCX8974784.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFALL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVILNRWTQSEK
                     "
     gene            complement(23407..24114)
                     /gene="deoD"
                     /locus_tag="O0V33_000084"
     CDS             complement(23407..24114)
                     /gene="deoD"
                     /locus_tag="O0V33_000084"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCX8974785.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIVAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            24350..25105
                     /locus_tag="O0V33_000085"
     CDS             24350..25105
                     /locus_tag="O0V33_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCX8974786.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLDRV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            complement(25207..25872)
                     /locus_tag="O0V33_000086"
     CDS             complement(25207..25872)
                     /locus_tag="O0V33_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCX8974787.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPVTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMNQNTHMN
                     QQPHMQQGHMQSSNHQMNQSNKKVLPAAGESMTSSILTASIAALLLVSGLFLAFRQRS
                     TNK"
     gene            complement(26241..26840)
                     /locus_tag="O0V33_000087"
     CDS             complement(26241..26840)
                     /locus_tag="O0V33_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCX8974788.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATILFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
     gene            complement(27108..28538)
                     /locus_tag="O0V33_000088"
     CDS             complement(27108..28538)
                     /locus_tag="O0V33_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCX8974789.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYM
                     VVITIFIDDYYLIFLLQGIYIIGAELDISWFYAGTEKFKIPSLSNIVASVIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMCIQQ
                     SHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVMNLVLCLVLIYFYGIYGAAIARLITEFF
                     LLIWRFIDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRHLRHKTI"
     gene            complement(28528..29766)
                     /locus_tag="O0V33_000089"
     CDS             complement(28528..29766)
                     /locus_tag="O0V33_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCX8974790.1"
                     /translation="MENQHNSKYLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMLVIMLILLCFTFYNINYYLFQLSDLDALPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMIYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            complement(29747..30913)
                     /locus_tag="O0V33_000090"
     CDS             complement(29747..30913)
                     /locus_tag="O0V33_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCX8974791.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHHDVYVIVGNHGPMIE
                     QLDARVNVIIIEHLVSPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRVVFTAHGWAFTEGVKPAKKFLYLVIEKLMSRITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGEVVGMLPNKQDLQINDSTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVVQAGLENDVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVTDNGICIV
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            complement(31123..31812)
                     /locus_tag="O0V33_000091"
     CDS             complement(31123..31812)
                     /locus_tag="O0V33_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCX8974792.1"
                     /translation="MKKLQRKKWKCREIGIEAVRNKGYAYQRKLDTLEEVKKGYFPIK
                     RAIDLVLSIVLLFLTFPIMFIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMCK
                     NAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVEL
                     FSSEIIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIRT
                     LMVVITGEGSR"
     gene            complement(31775..32755)
                     /locus_tag="O0V33_000092"
     CDS             complement(31775..32755)
                     /locus_tag="O0V33_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCX8974793.1"
                     /translation="MEDLEKVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LKLLETIKKYNSHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAVGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHDFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            complement(32825..32946)
                     /locus_tag="O0V33_000093"
                     /pseudo
     CDS             complement(32825..32946)
                     /locus_tag="O0V33_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(33090..33866)
                     /locus_tag="O0V33_000094"
     CDS             complement(33090..33866)
                     /locus_tag="O0V33_000094"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HCX8974794.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            complement(34077..34703)
                     /locus_tag="O0V33_000095"
     CDS             complement(34077..34703)
                     /locus_tag="O0V33_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCX8974795.1"
                     /translation="MEKNVEKSFIKIGLYFQIAYIVLMAITLCGFVICYGLIFGLFYL
                     LSGSRADYLIVTIVISAIISIFVIILSIVPVIVLASDLFKERISNGVILIVLAIIALV
                     LCNFVSAILWFVSAISILGRKKLVSTSDTTTIQKSKGNANQASHKDTCKKELDSQDMM
                     EHPEFKNPTTKNLEGLNEEVHKDEATTKVDSDNTEPPIESKDHVSKKD"
     gene            complement(34899..35663)
                     /gene="sbnI"
                     /locus_tag="O0V33_000096"
     CDS             complement(34899..35663)
                     /gene="sbnI"
                     /locus_tag="O0V33_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HCX8974796.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
     gene            complement(35667..36869)
                     /gene="sbnH"
                     /locus_tag="O0V33_000097"
     CDS             complement(35667..36869)
                     /gene="sbnH"
                     /locus_tag="O0V33_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HCX8974797.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERTILDTISQYVEGFEVASQGEIAKGLVFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALAMPNIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKAWSEKHQF
                     PLKHINLGGGIGVNYADLTSQFEWDNFVENFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFDIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREIQIEAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            complement(36869..37645)
                     /gene="sbnG"
                     /locus_tag="O0V33_000098"
     CDS             complement(36869..37645)
                     /gene="sbnG"
                     /locus_tag="O0V33_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HCX8974798.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDED"
     gene            complement(37620..39470)
                     /gene="sbnF"
                     /locus_tag="O0V33_000099"
     CDS             complement(37620..39470)
                     /gene="sbnF"
                     /locus_tag="O0V33_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HCX8974799.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMYQLVSS
                     LIYENIVVYKASYQDGVGYFTIEGNDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDTYLKDELKTAFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGVPFIENWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEKLQWKWVKGIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGITDATTISET"
     gene            complement(39379..41115)
                     /gene="sbnE"
                     /locus_tag="O0V33_000100"
     CDS             complement(39379..41115)
                     /gene="sbnE"
                     /locus_tag="O0V33_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HCX8974800.1"
                     /translation="MQNKELIQHAAHAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGQFAYWSAMGHHMYHPEVWLIDGKSKKLTTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYAEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPIHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDSVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPTSKLSQVIEQSGLAPE
                     AWLECYLDRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPEVCYVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWKLV
                     AHRLMTWKKEYANNAVFIDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            complement(41105..42361)
                     /gene="sbnD"
                     /locus_tag="O0V33_000101"
     CDS             complement(41105..42361)
                     /gene="sbnD"
                     /locus_tag="O0V33_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HCX8974801.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNVEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVIGQIIGSLSGAAITSYTTPATTFIVMGIVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            complement(42354..44108)
                     /gene="sbnC"
                     /locus_tag="O0V33_000102"
     CDS             complement(42354..44108)
                     /gene="sbnC"
                     /locus_tag="O0V33_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HCX8974802.1"
                     /translation="MQNHTAVNTAQTIILRDLVDALLFEDIAGIVSNSEITKENGQTI
                     LIYKRETQQIKIPVYFSALNMFRYESSQPITIEGRASKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTQMIHGDTANIDELESLTAPIKEQATDMLHDQGL
                     SIDDYVLFPVHPWQYQHILPNVFAKEIREKLVVPLPLKFGDYLSASSMRSLINIAAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAERLLRQLIEKDAMLAKYVTVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KKDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            complement(44129..45139)
                     /gene="sbnB"
                     /locus_tag="O0V33_000103"
     CDS             complement(44129..45139)
                     /gene="sbnB"
                     /locus_tag="O0V33_000103"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HCX8974803.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIENDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDTPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            complement(45136..46116)
                     /gene="sbnA"
                     /locus_tag="O0V33_000104"
     CDS             complement(45136..46116)
                     /gene="sbnA"
                     /locus_tag="O0V33_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HCX8974804.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            46347..47339
                     /gene="sirA"
                     /locus_tag="O0V33_000105"
     CDS             46347..47339
                     /gene="sirA"
                     /locus_tag="O0V33_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HCX8974805.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDSKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            47355..48350
                     /gene="sirB"
                     /locus_tag="O0V33_000106"
     CDS             47355..48350
                     /gene="sirB"
                     /locus_tag="O0V33_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HCX8974806.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALTGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYLMKKGRQRL"
     gene            48347..49345
                     /gene="sirC"
                     /locus_tag="O0V33_000107"
     CDS             48347..49345
                     /gene="sirC"
                     /locus_tag="O0V33_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HCX8974807.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMSGSSFIPLQRVLMYF
                     INPNDSIDQFTLEVLRLPRITLAILAGGALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVIGGAVAMVILLVFQTKGHIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            49714..50466
                     /gene="sarS"
                     /locus_tag="O0V33_000108"
     CDS             49714..50466
                     /gene="sarS"
                     /locus_tag="O0V33_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HCX8974808.1"
                     /translation="MKYNNHDKIRDFIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLF
                     HQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQRE
                     KIAERVTLFDQIIKHFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVE
                     FTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILI
                     HMDDAQQDHAEQLLAQVKQLLADKDHLHLVFE"
     gene            50887..52245
                     /gene="spa"
                     /locus_tag="O0V33_000109"
     CDS             50887..52245
                     /gene="spa"
                     /locus_tag="O0V33_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HCX8974809.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAKKLNDSQAPKADAQQNNFNK
                     DQQSAFYEILNMPNLNEEQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     EQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
                     EQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNNKP
                     GKEDGNKPGKEDNKKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAADNKLADKNM
                     IKPGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGAALLAGRRR
                     EL"
     gene            complement(52574..54166)
                     /locus_tag="O0V33_000110"
     CDS             complement(52574..54166)
                     /locus_tag="O0V33_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCX8974810.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPARVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISEDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFIVDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLEASEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICVWTFILTLIF"
BASE COUNT        17314 a        10222 c         8209 g        18555 t
ORIGIN      
        1 tcccttatat ttaaaatgaa tttattttta tagtgttttt ctaaaaaact tttaaataag
       61 tgtcctgtat aacttcccca tactatgaca aaatcataat gattttcttt gagaattttc
      121 atagcgtatg gcctaaatcg atagtaagca atagcttttt tgataaatgt ccaatcttct
      181 ttaatatcta ctttatattt atagtaatta ttgatgcctt ggatattttc ttcgatatca
      241 tatttgtcag tgtatataat gtccacctca ttgatattca aatcaatgtg gtttaaataa
      301 tgtgataata atgacatatg cttaatgtta gtagcgccaa gaatcgctat cctcatcgtc
      361 atttcctcca agtatttcga ttgataatat ctttatagct ttgaataatt ttaactactt
      421 ttgtcgaaac gttagtgtct ttataatcaa tagcatcaat catcggttcg ttattgtttt
      481 gcatctctct tgctagttca acggattgga ttagattgtt ataggtaata ccacctataa
      541 taaccgtacc tttatctagt acttccggtc tttctgtgga agttcgaata aggacaccag
      601 ggaacttcaa aatagacgac tcttctgaca atgttccact atctgatagc acaacaaatg
      661 catctttttg caatgcatta taatcaaaga aaccaaatgg ctttaactgt ttaactaatg
      721 gatcaaattc aaatttactt tcttcaattt tcttccaact tcttggatgc gttgaataaa
      781 tcacaggaat tttatacttt ttggcaatat catttatcgc attcattaat gatttaaaat
      841 tcttttcatt atcgatattc tcttctctat gcgcagatac taaaatgtat tgttgcggtt
      901 ctaatcctag tttatttaaa acgtcactgt gattaatttt atctcgatgc gcttctatca
      961 cttctgtcat cggtgatcct gtcacaaaga tattcgcttt attgaagcct tcatctaata
     1021 aataccgtct gctatgttct gtataaggta gattcacatc actgacatgg tcaacaattt
     1081 tacgattgat ttcttcaggt acattctgat caaagcatct attacccgct tccatatgga
     1141 acacaggaat ttttaatcgt ttagcagata ctgctgctaa acaactattt gtatcaccaa
     1201 gaattaaaag tgcatctggt tgttcgcgta ataaaacgtc atatgtcttc gcaataatat
     1261 tccccatcgt ttctccgagg ttacttccaa ctgcctctaa gtagtggtcc ggttgtctta
     1321 attccaaatc atcaaagaaa atttgattca atgtataatc ataattttga ccagtgtgca
     1381 ctaatatctg attaaaatat tgatcacatg ctttaatcgt tgatgataaa cgaatgattt
     1441 caggccttgt accaactatt gtcattaatt ttagtttttc catgcgctat acctccaaga
     1501 aatacgtatc tggctgattt ggatcaaaca tttcattcac ccacataata gttaccatat
     1561 ctgtgtcgcc taaattttca atattatgtg tgtatcctac tggtatgtct acaacttcta
     1621 atttgtcgcc agaaacgtaa tattcaatga tttcattatc attaacatgt ctaaaacgaa
     1681 ttaccccttt acctgatacg actagaaatt tttcgttttt agtatgatgc cagtgattac
     1741 ctttagtaat tcctggttta gaaatattta cagaaacttg accacgatcc ggtgttttta
     1801 taaattctgt aaaagatccc ctatcatcca cattcataag taagggataa ctaaagtctg
     1861 tactaggtag atagcttaaa tacgtactat acaaatcttt ttcaaacaag ttatctaatt
     1921 tcggcaatgt tcgatcgaga cgtgactgtt tgaacttgta taataaatct acaatttctc
     1981 ccaatgtcac tttaaataca tttggtactg taggtacacc attttcaatc gttggatttc
     2041 cttcaatagc acgttttatt tcagcgacga tatcatccac gtagtttagc gttagttcaa
     2101 cattccgatt attaacttga atctcttcgt tacgtgcaat tttgtaacaa aatgttgcta
     2161 tcacagagtt ataattcggc ttacaccact taccgaataa atttggccat cgataaatat
     2221 aaaccgtatt gccatactct tcggcatact ctcttaatag ctgttcacct tgcaacttac
     2281 tctcaccata aggattatct tgtgttgctt gtattgaaga cgataataat atcgctggct
     2341 ttttcgtatt tctagttaat atatcaagta catgatctaa ataactcacg tttcctaagc
     2401 tgaattcttt gtcatgttca ggtcgattaa ctcccgctaa atgcacgaca aagtctgctt
     2461 tcaacaacgc tgactctaat tcttcctctt tagtttgtcg atgtacttcg aaaatatgat
     2521 gatctgtcgt tgatgttaaa tctgctttca agttttttcc tacaaaaccc tttgctcctg
     2581 taattacaat attcaacgtc aatctctcct atctcattga agctttataa tcattcaatt
     2641 cgtttctaac atattctagt gttaaaagtt tttcttttat ctcttccacc gttaaaatat
     2701 gtgtattatc ggagttatat tcataagatt gcgtaatctt ttcgttaccg gtttcaacat
     2761 aattactata atttaaatct ctggagtctg ccggcactct aaaataatca cccatatctt
     2821 cacattgtgc gtattcttct ctcgtcaaca atgtttctgc ttttttctct ccatgtcgcg
     2881 taccaatgat ttcaattgca ttatcagctt caaacaattc taataatgcg gtcgcaagat
     2941 cccctaccgt tgaacttggt gctttttgaa ccataatatc gcctgtctct gcatgcttaa
     3001 atgcatgaac aactagttct accgcatctt ctaagctcat taaaaatctt gtcatatcag
     3061 gatctgtaat cgttaaaggt tctccagctt tgattttatc gataaacaaa ggaattactg
     3121 aacctcttga agccattaca ttaccgtatc ttgtaccgca aataagcgtt tgttcactac
     3181 gaatatttct tgatttagct acgaatactt tttccatcat tgctttcgaa atacccatag
     3241 cattaatagg atacgctgcc ttatctgtag ataaacatat gactttttta acattttgat
     3301 gaatagcact ttgtaagaca ttttctgtac caataatatt tgtcttcact gcctcaactg
     3361 gaaagaattc acatgacggc acttgtttta aagctgctgc atggaatacg taatcaacat
     3421 ctcgcattgc tgtttctaca ctttgactat cacgcacatc accaatgtag aactttaatt
     3481 ttgaattatt atattttttt cgaatgtcat cttgtttttt ctcatcgcgt gaaaaaatac
     3541 gaatttcttt aatattagaa tctaaaaacc gtttcataac agcattaccg aatgatcctg
     3601 tgcccccagt aattaataaa attttgtcat cgaacataat tatctccctt tttgccattg
     3661 gcatagttaa taattttttc tttactaaag tcattgacga tgtcttgaat aatcgcttca
     3721 acttcattac atttcatatg ttgtactttg ccacgataaa ttttttcaaa tacttgttca
     3781 gggtgaattt catccttatt catcaattct tcatacattt tttcaccagg tctaatccct
     3841 gtaaaagtaa tgcgtatatc gtcttctttc ttaccactta acttaattaa atttcgtgct
     3901 aaatctacta ttttcactgg ttctcccata tctaacacaa atacttcgcc accttctgct
     3961 aatgcccctg cctgcaaaac tagtctagaa gcttcaggaa ttgtcataaa gtaacgtgtc
     4021 atttcaggat gtgtcacagt aactggccca ccttcttcaa tttgactttt gaaaagtgga
     4081 atcacagatc ctctcgatcc aagtacatta ccaaatctca ctgcaacaaa atttgttcga
     4141 tgcgtttcat catttaaact ttgaataatc atttctgcaa ttcgctttga agctcccatg
     4201 acattaggtg gattaacggc tttatccgta gaaatcataa cgaatttctt tacctctgca
     4261 tttttagcag cttcagcagt atttttcgta cctaaaatat tattacgtac tgcttcttca
     4321 gggttgtctt ccattaacgg cacgtgcttg tgtgctgctg catgataaac tgcgtatggt
     4381 ttatacattt ccataatttc aaacatacgc gctctatttt gcacatccgc tataatagga
     4441 acgatatcaa cattttttcc gaagcgattt cgcaattcac gattgattaa ataaatactg
     4501 ttttcaccat ggccaagtag aataatacgt tctggataga aattacaaac ttgtctacaa
     4561 atttctgatc ctattgaacc tccagcgccc gtaactaaaa tagttttatt cgtcaattca
     4621 tttgatatca tatccatatc taattcaaca ggatctctgc caagtaaatc ttctacttca
     4681 acttttttca gttggttcac ttctaactca ccagacatga cgtcttctat atttggcatt
     4741 ttcaataact ctacgccatc catatggcaa atattattaa tttctttcaa acgctcttga
     4801 ccaatagttg gaattgcaat gataattttt ttaatcttat atttcctcac tagttctgga
     4861 atatccgcga ttttaccttg gacttttaca ccctcagtaa ttgtgatatt acgtttatgt
     4921 tcgtcatcat cgactgctaa taccggttca agtttcattt cgtcactttt caacatttgt
     4981 ctaatcagca ttgaacctgc ttgaccagca ccaacaacta aagttggctt cttattaaat
     5041 gacttacctc caaggtattt ccgataaata cgccaaaata accttgagcc acctattaaa
     5101 atcaagtgca tcatccatgt aattaaatac aacctaaaaa acggtctatt gcctgtaaca
     5161 attgtcacga acaccatcgt aataacgata gatgtcgtca cagctttaac aattaaaatc
     5221 aattcactca cactggcata ttcccacgct cgatgataca tattaaaaat aaatgctgaa
     5281 atatgatgcg atatgaatag tgatatagct gccaatatta ataatttgac agaatatgtt
     5341 ttgaaatacg gttctaaaat gtaataactt acgaatactg aaaatgtcac tatcagtgaa
     5401 tcgattaatg ctagtattaa aagccgcaat ttcacagata aatgtgccat aaaacccctc
     5461 atttgctgtt tataacccaa accatctttt ctgtttataa tcttgttgtg gcattcgttt
     5521 aggaattttt ttatcatcaa caactaactt cgcattacta ataaatccgt tcatatcttc
     5581 ataataatca cgtaatttct tatcattaaa taagtctttc attaagaacg gtctgatttc
     5641 tgtgttatgc gcatctgaac cgatgaaatg tgtcagattg ttttcaatca tttgaattgc
     5701 taattttcta atttttttac cggaaatacc cgctaatgac gccgttgtca cttgacttaa
     5761 agcacctttg ttaattaaat cgtatagtat gtcaaggttt tgacttattg ctttattccg
     5821 ctctggatgt gcaataatcg gtacaaagcc tttactctgt aattcgaaaa ataattgatc
     5881 agtatagtgt ggaacttcat ttgatggaaa ttctattagt aaatagcgtg aatcattaat
     5941 accggtaata acttttcgat caatatcatt aaggatttga tcggtaattc ttatttcctg
     6001 accataataa aactttagat ttagtgcttg tacttcctct aagctttcaa tatggtttaa
     6061 acatgatttc actttttcta taggtgtggt atatcgagga tgtaagtgat gtgatgttac
     6121 aatgacttct gtaacacctt gtgttgtcgc ttgtcttaaa agatccagca tctctgtttc
     6181 atttgtcgga ccgtcatcga tattcggcaa tatatggtta tgaatatcaa tcatacctat
     6241 tcatctccat aatagtgata ataactagaa gacttatcta cttttgcctt atttaaaatg
     6301 acgcctagaa tattactgcc tgctttttcc ataagtgttt tagctttttt aacttcattt
     6361 ttatcatttt tttcactatc aatgactaac agactatctt taatagcacg tgcatatagt
     6421 tgtgcatcag tcacggtatt aactggcggt gtatcgacaa taatgatgtc ataacgctca
     6481 ttgaaaagat taactaactc tttaaatctt tcagaaccaa ttaattctgt tggattagga
     6541 ggtacaggac cagctgttag taaatctaaa ttatcaattt ctgtcgtcgt aatagcttct
     6601 gacatagtcg ttctaccaat gattaaattc gataatccat tattattttg ttcattaaaa
     6661 atatagtttt gtgttggctt acgcatatcc ccatcgataa ttaacgattt ataacctgct
     6721 tgtgcataag taatcgctac attggatacg actgtacttt tacctgcacc aggcttttca
     6781 gaagtaacca ataagcgctt tacttcacca tttgcttttg aaaacatgat gtttgaacgt
     6841 atacctcgaa acttttcact aattgttgat tttggttttt catacacaaa tagtgttgtt
     6901 gtcgtatttt ccttttttga cataagtagc aaccatcctt aattaaattt ttgaattgaa
     6961 cccaatacag gcaatcctaa ttctttctct acatcttctt catctttaat tcgcttatca
     7021 aaaattactt tgaagaagat atatataagc gcgacaacta atcctaaaaa gagtgcaccg
     7081 attagattca ctacagtttt tggtgcgact ttaactgctg taccgtctgc tttagataaa
     7141 attgatacgt tatccacact cataatcttc ggaatttgtt tacttgtaac tttagcgaag
     7201 ctattcgcaa ttttttccga atcttgttta tgaccacttt taacttggat gttgataagt
     7261 tgtgtatttt cttggtttgt aattgtcaac atactcgata atttagatgg tgaatactta
     7321 tcatttaagt cctttgacac ctcatctaaa attctaggac ttttaacaat ttctttatac
     7381 gtatttacaa gttgaatatt actttgaacc tcttgcgcca taaactgagg attgtcaccc
     7441 ttagtttgat tcactaaaat ttgagtatta gcttgatatt taggtgataa gacgaaaaat
     7501 gtaacaatag cgctaataat taaaaataat agcggtaaaa taattaaaat cttcaagttt
     7561 ttttgtaata cttctttaat ttttgttaat tctaatgtac tttccattat ttacctccct
     7621 taaaaatttt cattaaaatt ggtattatat atatagtatt tacatattac atatcgttta
     7681 aacaattaat tacttttaaa ccgcattatt ttcgcctttt tatttatatg aaattaaaaa
     7741 taattatact ctaaagtagt aatagtttgt gaaaagtgta ccaatcttaa tactttcttt
     7801 attaaaaaaa ttgattgtct aggttgtatc tctctgaaga tttggcaata aagaaaagcc
     7861 gataaccgta taatgattat cgactttaag tttatgtggc attttttact tttgtaattt
     7921 caggtgagtt agatgattat tatcagatag attattgctt ataatcatat gatgtttgaa
     7981 tgatatcttt gatttcactg attagcgctt ctttaggatt agcagttgta cactgatctt
     8041 caaatgcgag ctctgccatt ctatcaattg actcatttaa ttcttcttca gacacacctt
     8101 gtgatttcaa attcatttca attccgactg attgacctaa ttcgtagaca gctttagcta
     8161 atgattctac gagtgcttct gtcgtattac cttttaatcc taagaatttg gcaatatctg
     8221 cataatctgt atctgctctg aagaactcat atttagggaa taatgcatgt ttttgcgggt
     8281 ctttggcatt ataacggata atatgcggta gtaatatcgc attcgctcta ccatgcggaa
     8341 taccatattc cccaccaatt ttatgcgcaa ttgagtgtgc aatgcctaag aatgcatttg
     8401 caaatgccat accagccaaa gttgatgcgt tatgcatttt ctctcttgaa actttatcac
     8461 ctttttcaac agatgatttt aaatattcga acgtcaattt aatcgcttgt agactcaaac
     8521 ctcttgtgta atctgaagcc attacagata catatgattc cattgcatgc gttagtacat
     8581 ccattcctgt atctgctgta acgctttttg gcacactcat cacaaattga gggtcaataa
     8641 ttgcaacatc aggtgttaaa gcaaaatcag ccaacggata ttttacattt gtttcactat
     8701 ctgtgataac tgcaaatggt gttacttctg aacctgtacc tgatgtcgta gggatacaaa
     8761 tgaacgtcgc attttcaggc atgcctattt tataagtacg tttaccgatg tctaggaact
     8821 tttgtttagc accgaagaat gatgtctcag ggtgttcaaa gaacatccac attgcttttg
     8881 cagcatccat cgctgaacca ccaccaagtg caatgattgt atccggttgg aaatcaacca
     8941 tcatttccag acctttatat actgtattcg ttgatgggtt cggttcgact tcgctaaata
     9001 ttttaatttg aggctgttcc gttctttgtc ttaatacatt ctcaactgtt tttgtataac
     9061 cgaattcgac cataccaggg tcacagacaa tcatcacttt ttcaatcttg tccattgttg
     9121 ttagactcat gattgcattt tcttcaaaat aaatttgagc aggcaccttg aaaatttgag
     9181 tattattacg tcgtttagca attgttttaa tgtttaataa atctgtcgca ctaacattat
     9241 gtgaaattga gtttctaccg tacgaaccac aacctaatgt taaagacgga atcaattcgt
     9301 tatacatatc accaatacct ccaaccgctg atggtgtatt tacaagtaca cgacaagctt
     9361 tcattcttag tccaaaatct ttttgtaatg tttcatcttc tgtatggata acggctgtgt
     9421 gtcctaatcc accaaaatgt agtgtgtctt cacaaatttg aaatgcttgt tttgtagatt
     9481 gggcttttac taaggctaat actggagata atttttcacg agataacgga tagtctgaac
     9541 ctacaccgct aatttcggct atgataagtt ttgtattttc ggggacaggt atacctgcta
     9601 attcagctat ttcaactgca gatttaccga caatatcagg tttaatactt gttttttgtt
     9661 cattcataat tgcattttct aagcgttgta attcatcttt tttaacaaag tatgcttgat
     9721 gtgctttaaa ttcattagta acatctttat aaatttcttt atcaatgact acaacttgtt
     9781 cagaagcaca aatcatacca ttatcaaatg tttttgaacc aatgatatca tttactgcac
     9841 gcttaatgtg tgctgttttt tcaatgtaag acggcacgtt acctggtccc acacctaatg
     9901 ccggtttgcc agttgaatat gcagacttaa ccatgcccga accacctgtt gctagaacta
     9961 atgcaatacc tttgtgattc attaattgtt ttgttgcttc gatagagggc acttcaatcc
    10021 attgaataat atctttaggt gcacctgcct tcattgccgc ttctaataca acttctgctg
    10081 cacgcttcga cgattcttgc gcacttggat ggaatgcaaa aatgattgga tttcctgtct
    10141 taattgcaat catcgcttta aaaatagttg tcgacgtagg atttgttgtt ggcgtaacac
    10201 cacaaataac accaattggt tccgctacat acgttaatcc tttttcttta tcttcaccaa
    10261 taatccctac tgtcttattg tcttttattg aattccatat atattcagaa gcgtataaat
    10321 ttttaatcgc tttatcttcg tatatacctc ttccagtttc ttcatgtgct aattttgcta
    10381 gcaccatatg ttgatcaaca gctgctaagc tcatttgatg aacaatatga ttaatttctt
    10441 cttgtgactt tttagataat gcttctaatg cctttttccc tttgtcagct agagcatcaa
    10501 tcataattgc cacttcttgt tctttcgatc cacgattttc tttttcaggt atagttaaca
    10561 tatacaacca ctcctttata ctttgtgaat tatttcacaa acattatagt acatgtctct
    10621 caggatataa agaaaattct atacaaaaaa gtttaatttc gaatattatt tgaacaaata
    10681 tcaaatttta aaataaatgt tttcatgaaa tcattgttat ttcggtgttt ttagaatgat
    10741 tttataatca taattttttc aatgacataa tttattcata attatatatt taattctgaa
    10801 tattctttaa tagcctaaaa agcttgttat atcgcacaaa acacaacatt aaaaggtcct
    10861 tgtcgatacc tatcaacaga tgttgcaaat aaaaacctcg aactgaaatg ataatctttt
    10921 cagctcgagg tttaaatatt ggtgtcttat ttatatagat tcgttatatt atattctcta
    10981 ttttcattaa cataatcctt aaagagtttt aaattaatac ctgctagatg attcaaaaat
    11041 gtttcatcaa cttttaaata attcaataat ttttgtggtg tcagtatatt tctatcaaaa
    11101 tacaacttta ataaactatt cattttgaca ggacgtgaca tttcaatcac gtcgtctaaa
    11161 ggctctttac ttttatagcc ttttttattt aatgatgccc aaaaatatct gtactgagta
    11221 ctagacatta gacctagata ataggctctc attgcaattg cttgtataga aacataccat
    11281 ttttctttca atacagctaa ataatcagga tttgattttt ttgtcatttg atcaaagtct
    11341 tttttaaacg cttcttcagg tagtaaaaat tcagaggcga aaatatctgc ctcatgttca
    11401 attgtcttat attcttcagg agataataaa tcgaattgaa tatgacgatg tagaacgaga
    11461 tgtcccagct catgtgcaag atcaaaattc cttctaactg ctaccccctt atttgtaccc
    11521 agtataataa aaggtgtctg atctttactc caaaaactat atgcgtctat actatcatta
    11581 attcgctttt cgtagataac tatacccgct ttttcaagca taaataatag tgcttcattg
    11641 ctttcgtctt gaagaatttt agccctcaca actttagcag tttcttttat gctttctttt
    11701 ttatttagtg attctaaatt gccattcaac aagtcatcta aattatttat taaagataaa
    11761 ataatattat ccggtatctt tacaaaggaa aataagtagc tagttaaatt agataaatac
    11821 attgcttgat aatattgctt atttaatagt ttcgtagata ctttataatt ttttgctcta
    11881 aaagctatac tatgtttatc gacagaattt attaggagct cttcgggctg ctcgctaata
    11941 aagtagctag atttaacatt aaaaatagat gtcatatcat aaattttatt aatttctggc
    12001 atcatatttt ttgtttcata ttgccaaatt gcttgttcac ttacatttat tttttctgat
    12061 aattcttttc tacttagagc attaagcttt ctcacgtatt ctaaattctt gccaataaac
    12121 attaacttca cctttcgctt tcaatcatat cgttatcttc atcgatactg ataccaaatg
    12181 catgttctac atctatgaat tctaactctc catcgttttt aagtacttct aactcttctt
    12241 cgtcaatatg ataatcttct ctttctacta ttactttgtc taacaatggt gtcaaatcat
    12301 caaccataat tgcagaattt gtttctggat ttggcaacca ttgtttgatt gcagctaatt
    12361 gctttgattc tttatcaata cgatatgtaa taattaaaaa ataagaatta atatctctat
    12421 tactgccttc ttttatatta aataatatac tatctgcagt catttgatgc ctaattttaa
    12481 aatcttctcc aacagtatca aaattaatgc cactattctt agctataagt ccctctaggt
    12541 agtttttact tttttgattc ttatcagcaa ctaaactctt ccctacgctt acattttttt
    12601 catcaaagta atcttcattt ttcaatataa acatgatatt atttttctct tcatccttat
    12661 taaatataaa ctttaaatac ttccatgatg gtccagcatt ttctttaata tacttaacaa
    12721 aattttccaa atgcttagca acttgatttt caatatgatt agatttggtg tacacatagg
    12781 catcactaat caccatacgt ttcttgactt catttctctc attaacatag tccttatatc
    12841 ccgcgaaaat tcctttaaca atagattctc ttacttcttg tttaattgtt tccaaattaa
    12901 tcacttcata atattaagtt atataaaata taagccttgt ttttttaaaa atcaacattt
    12961 ttcttaaagt atattggtgt ttaagagtga ttagtttaaa caaaaacaaa aacttacccg
    13021 attaaaatca agtaagtttt acatatatcc gtcacttttc aactttaaaa tcaacaactt
    13081 gcgggatgcg catcaattta ttatttgata aaaaatcaat aagtaattgt gcgccttcaa
    13141 cttgaatatc ttttacaact ggcgcgtcga tatacatatc atactgacca ccgccaactg
    13201 cacgataatt atttacacaa attgtatatg tctgctttaa atcaactgcg tgaccttgaa
    13261 tcatcatatt gctcacacgt tgtccctttg gtcttccaac atgaatggta taacttacgc
    13321 caccatatat atcataatta aagtgttggg gtttgggttc aaggaagtct gcgctcacac
    13381 ttacttcatc atttttcacg tcaaaatatt ctgctgatcg ttcaatggct tgtttaagtt
    13441 tgaaaccact tacagctaaa actttaaatg tatttggaaa tgggtaattg ttaataacat
    13501 ctcgcatcgt cacgacttgc ttgaaaccac tagcagaatc aaacaaagct gtacaggcaa
    13561 catctgctcc acttttttct aataaagcgt aattcataaa attagtaaaa ggatgcggag
    13621 ctacacgtgc ctcaaatgca tgattaatca tcatatcata tggcaatgtt gtaatttcgt
    13681 aatctaacca gtcctctagc tgctttcgta tatgttggtc atcttcatca atagtaaatg
    13741 tgggatcatc tataacagga agtaattcac atgattcaac ggatacattt tcatattcat
    13801 cagtactcaa gacaactttg cctacagttg tacctctcgt accaggttga atcacagccg
    13861 tttgcttaaa cctttcagca atttgtcgat gttggtgacc cgtaataaag atatctatat
    13921 ctttagaaaa cgcttctaac atgccatatc cttcattttc acccgttaac acttcggtcg
    13981 gcgtaccact ttctatatcc ttttcaaatc caccatggta acaaaccaca atgatatctg
    14041 catatcgctt catttcaggt aagtgttgtt gaagtgttgc aaaagcacta tgaaacgtaa
    14101 gtgactgaat atgctctggt tgttcccaat ggggaataaa ttgtgtcgtt aaacctatca
    14161 caccaaccgt ttgatctcca acctgaaaat actgcacacc gttatcagtc aatgtactat
    14221 cattttcata aatattagcg cacaaaactg gataattgag tctgcgtaaa gtgtctttta
    14281 agtatggtaa tccataatta aattcatgat taccaagcgt accaaagtcg aatgccattc
    14341 gattataaaa atcaactaaa ggctggctac tgccgctatg cgcgattaag taattacaaa
    14401 atggtgaccc ttgcaaaaaa tcaccattat ctattttaaa actttggtca tactgccttc
    14461 tgtcttgttc tataacatga ttcgctagta acaatcccat aggctgatat tgatttctac
    14521 tcgtaaaatc tgttgggaaa atataaccat gtacgtcact cacgacataa aatgctatgt
    14581 ttgacatcct cactcactcc ttcaatcaca aacatctttc ttatttctat tatatattta
    14641 tttgaagtct gttgtaatca aggttttgtc accgagtttt aaacgaatct ttgaaccttc
    14701 catactttcg agtactttgg cattgacctt aattgtggca tttccgtttt catctgcttt
    14761 aactgttggc aaagtactgt aacctggcgg gttataatcg ttatctttac ttgaaaattg
    14821 tccgatttga cgtcctcctt ctttgtaaat accaactcta agattttcaa gtgtttgatt
    14881 tgcttcaaat ccttttaaat gtattgtcac ttcaaatggc tcatttacag aaacattttg
    14941 cgggatatca aatgatactt tttcggtctg atttggtggc gtatgatcat ctggtgtgtt
    15001 tggctgagga tccgcgcctt tttcactgcc ataactacct gctttaaatg ttgttgggtc
    15061 ataccattta taaccactcg gcggttgtga ccatggctct ttttcaggct cagttgaacg
    15121 ctctggtcgt tcaaaatcaa gcaacttagt ctttgtatct aatgttaggc cactcgcctt
    15181 aagtgatttc ccatcattat ctttagacat ccaagctgtt atattattta atagcttacc
    15241 gttgtcttgt tctttaaaac catcatatgt tttcttcttt tctccattat cttctctcac
    15301 atatttgggc gaactatctt ccacaagtga tgaatcaccg ataaatgctg ctttaccttt
    15361 tccaacttta gaaattgcta catagggacc ttctgcttta ccgcccccat tataaatacc
    15421 ttgatctaca gcatgtgacc atttactttt cgctggcaat tgttctggtg tatacacaat
    15481 accttttgct ttctctggat tagtaattgc taatgtcgaa ccggcatgca tagatacaga
    15541 tttcacacct tcagtaatac caaaactttc ttttgaagaa acaatattgc tcgtatttaa
    15601 atcacctagt gcattatatc gaaaacgtac gccaaagttt gtagataacc aatctgaact
    15661 tttcacacct tgcattgcag tagaactttt ttcttctgca ttcatacctt tcgacatatc
    15721 ttcatacgct ccacgtcgat aaccattcat tgcctctgat gaatcaatac gatttaaatt
    15781 tcggtcagca ttgtaatggt ctgaaataaa gacaacattt cccccttgtt tcacatagtt
    15841 aacaattgct gcctgttctg attctttgaa aggaatgtta gcctcaggaa ttacaaatat
    15901 tttggaactt ttcaaacttg cttctgttat gttcgaatga ccatcaatag ctttaacgtc
    15961 ataaccttgt ttttgtattg aatccgcata atctgaaaat gcaccatcac taacccaatc
    16021 tgcagcacca gctgtttgac catgagaacg atcgaataat actgtttgct gttgctttgt
    16081 aggttgcgat tcatgcgtta tagctaaaga ttgcggtaaa gcacttaatg ataccgttgc
    16141 aacaattgca gagacagtta atgacttata tatatttttc attttttgag gctcctttta
    16201 aaataaattt gttcttgaat tataggataa atatatgttt gaaatgggca atttaacgaa
    16261 aattttacaa aatcttatca cactcttaaa gaaagttatt aaaattcatt tttataaaat
    16321 actttttagc atttaaatgt ggtacgctat aagtgtaatt tcattgcata catattacac
    16381 gattaagaat gtgaagggga cagttatcaa atgaaaaatt ttaagtgttt atttgtatta
    16441 atgttagcag tcattgtttt tgcagcagca tgtggaaatt caagttcttt agataatcaa
    16501 aagaacgcta gtaatgattc ggattctaaa tcaggaggat acaaacctaa agaattaaca
    16561 gttcaatttg taccttcgca aaatgctgga acattagagg ctaaagcaaa accattagaa
    16621 aaattactat ctaaagaatt agggattcca gttaaagtgt ctgtatcaac taactacaat
    16681 acaattgttg aagctatgaa gtctaaaaaa gttgatgttg gtttcttacc accaacggca
    16741 tacacattag cacatgatca aaaagcagct gatttattat tacaagcaca acgttttggt
    16801 gtaaaagaag atggttcagc aagtaaagaa cttgtagata gttataaatc agaaattctt
    16861 gttaaaaaag actcaaaaat taaaagcttg aaagatttaa aaggtaagaa aattgcctta
    16921 caagatgtaa catcaactgc tggatataca ttcccacttg cgatgttaaa aaacgaagca
    16981 ggtattaatg caactaaaga tatgaaaatt gtgaatgtta aaggtcatga ccaagcagtt
    17041 atctcattat taaatggaga tgtagatgct gcagctgtat ttaacgatgc acgtaatact
    17101 gtgaaaaaag accaaccaaa tgtatttaaa gacacacgaa ttttaaaatt aacacaagct
    17161 attccgaatg acacaatttc tgtaagacca gatatggata aagacttcca agagaaattg
    17221 aaaaaagctt ttatagacat tgctaaatca aaagaaggtc acaaaattat tagcgaagtt
    17281 tattcacatg aaggatacac agaaacgaaa gattcaaatt tcgacattgt aagagagtac
    17341 gaaaaattag ttaaagatat gaaataatca ttatttaacc aatgaatcat tatcgaattt
    17401 ggtattaaaa gctttcgttc aatagatata ttctagatta atattgaaaa gctaggcgct
    17461 aaactgaaac agatatagaa aggtgtcgct gtacatttga aactatttgt acacagaaac
    17521 ccaatgtcta tgatatttca gtttaccttg gcttttcttt attaaagaaa ggtgtcaaac
    17581 atgagtcaaa tcaaatttaa aaacgtcagt aaagtctatc ctaacggtca tgtaggcttg
    17641 aaaaatatta acttaaatat tgaaaaaggt gaatttgcag tcattgtcgg actatctggt
    17701 gctgggaaat ccacgttatt aagatctgta aatcgtttgc atgatatcac gtcaggtgaa
    17761 attttcattc aaggtaaatc aatcactaaa gcccatggta aagcattatt agaaatgcgc
    17821 cgaaatatag gcatgatttt ccaacatttt aatttagtta aacggtcaag tgtattacga
    17881 aatgtactaa gtggacgtgt aggttatcac cctacttgga aaatggtatt aggtttattt
    17941 ccaaaagaag acaaaattaa agcaatggat gcactagaac gggtcaacat cttagataaa
    18001 tataatcaac gctctgatga attatcaggt ggccaacaac aacgtatatc tattgcacgt
    18061 gcgctatgcc aagaatccga aattattctt gcagatgaac cagttgcttc attagaccca
    18121 ttaacaacga aacaggttat ggatgattta agaaaaatca accaagaatt aggcatcaca
    18181 attttaatta atttacattt tgttgactta gcaaaagaat atggcacacg catcattggt
    18241 ttacgtgatg gtgaagttgt ctatgatggt cctgcatctg aagcaacaga tgacgtattt
    18301 agtaaaatat atggacgtac aattaaagaa gatgaaaagc taggagtgaa ctaacatgcc
    18361 tttagaaaca cctacaaagt atgactccct tttaaagaaa aaggtttctt taaaaacgag
    18421 ttttaccttc atgttaatca ttgtgcttat catttggagc ttcatttata cggggtttaa
    18481 ttttggagat ttaatgatag gaatacctca aataggtgat ctattcaaac aaatgattcc
    18541 acctgatttc gagtatttac aacaaattac aacgccaatg ttagatacca ttcgaatggc
    18601 tatcgtaagt acagtattag gtagcatcgt ttcaatacca attgcgttat tatgtgctag
    18661 caatatcgtt catcaaaagt ggatttcaat accctcgcgc tttattttaa atatagttcg
    18721 tactattcca gatttgttat tagcagcaat ctttgtggct gtatttggaa tcggtcaaat
    18781 tccagggata ttagcattgt ttattttaac tatctgtatt attggaaaat tattatatga
    18841 atcattggaa acgatagatc caggtccaat ggaagcaatg acggctgttg gcgctaataa
    18901 aataaaatgg attgttttcg gtgttgtacc acaagccata tcgtcattta tgtcatacgt
    18961 attatatgca tttgaagtaa atatacgtgc ttcagctgtg cttggattag tcggcgctgg
    19021 cggtattgga ttgttttatg atcaaacact tggtttattt caatatccaa aaacagcaac
    19081 gattatttta tttactttag ttatcgtcgt cgtcattgat tacatcagta cgaaagtgag
    19141 ggcacatctc gcatgaaaca ggaaatagaa aaatataatg ttcacacaaa agcacacaaa
    19201 cgaaaattga ttaaaagatg gcttattgca attgtcgtct tagctattat catctgggca
    19261 tttgcaggtg taccaagttt agaacttaaa agtaaatcat tagaaatctt aaaatccata
    19321 ttcagcggat tattccatcc tgatatcagc tatatctata taccagatgg cgaagactta
    19381 ttacgtggtt tacttgaaac ctttgcgata gccgttgtag gtactttcat cgccgcaatt
    19441 atctgtattc cattagcatt tctaggtgca aataatatgg taaagctacg cccagtttca
    19501 ggtgttagca aatttatttt aagtgttata cgtgtcttcc cagaaattgt aatggcactt
    19561 atatttatca aagctgttgg cccaggttca ttttcaggtg tattagcttt aggtatccat
    19621 tccgtaggta tgcttgggaa acttttagct gaagatattg aaagtctaga tttcagtgct
    19681 gtagaatcat taaaggccag tggtgcgaat aagattaaaa cactcgtatt tgcagtcata
    19741 ccacaaatta tgcctgcctt tctatcactc atactttatc gctttgaact aaacttacgt
    19801 tcagcttcta tactggggct aattggggct ggtggtatcg ggacaccact catatttgcc
    19861 attcaaacac gttcttggga ccgtgtaggt attatattaa tcggtttagt actaatggtc
    19921 gcaattgtcg atttaatttc cggttcaatc cgaaaacgta ttctttaaca ttaaatcagg
    19981 atactcctga ataagaggtc ctaccgtctt acgtttctct attataataa aaacagcagt
    20041 gaagaaaact attattatag ttaacttcac tgctgttttt attatatcta aatttattct
    20101 attttaattc ctttaaataa cttttaccga actctggtaa tgttacgttg aaattatctg
    20161 ctatagttgc accgatagag ctgaatgtag tatcactttc tagtgcatga ccacctttaa
    20221 atttcggact gtacataatt actgggatgt attctctcgt atggtccgta cctggcgctg
    20281 tcgggtcatt accatggtct gctgtaataa ttactaaatc gtcttctttt aagttgctaa
    20341 acagttctgg caagcgatca tcgaaatctt taattgcttg tgcataaccc ggtttatcac
    20401 gacgatgacc gtataatgca tcaaagtcta ctaagtttaa gaagctaata cctgtgaaat
    20461 ctttcttaac aattttcatc aattgatcca taccgtccat gttactcttt gtacgaaccg
    20521 cttctgttac accttcacca tcataaatgt cattaatttt accgatggca atgacatcat
    20581 aaccaccgtc tttcaaatga tctaagacag ttttaccaaa tggttttaac gcatagtcat
    20641 gtcgattaga tgtacgtgta aagtttcctg gttcaccaac atatggacgt gcgataatac
    20701 gaccaattaa atatttaggg tcttttgtca actcacgaac cttttcacaa atatcatata
    20761 actcttctaa tgggataatg tcttcatgtg cagcaatttg caatactggg tctgcacttg
    20821 tataaacaat taagtcacca gttttcattt ggtgctcgcc ccactcatcg ataatttgcg
    20881 tacccgatgc cggtttgtta gcaacaactt tacgacctgt catttcttca atttgttgaa
    20941 ttaactcttc agggaatcca ttagggtata ctttaaaagg ctgcataata tttaatccca
    21001 taatttccca gtgaccagtc attgtatctt taccaactga agcttcactc aatttagtat
    21061 agtatgcttc tggttgttca actgcattta ctactggtaa tttatcgatg ttccctagac
    21121 ctaacttttc aaggtttggt aaagtttgat cgaagccttc taaggtatgt cttaaagtat
    21181 gtgaaccttc atctttaaaa tcagctgcgt ctggcgcttc accaatacct actgaatcca
    21241 ttacgattaa atgtacacga ttaaatggtc ttgtcatagc tatcactccc aaattttata
    21301 tatattagta atctgaatct gcttctaaac cttgcataat ttgaacgcct gcgctcgcac
    21361 caatacgtgt cgcacccgct tcaaccattt tattgaaatc ttctaaatta cgtacgccac
    21421 ctgatgcttt tacttctaca tctgcaccta ctgtatcttt cattaattta acgtcttctg
    21481 cagtcgcacc gccacctgca aaacctgttg aagttttaac gaagtccgca ccagccgctt
    21541 ttgttaattc actcgctttt acgatttcgt catggtccaa caataccgtc tcaataatca
    21601 cttttactgt gtgaccttcc gcagctttaa ccactgcttc aatgtcttgt tgtacatcat
    21661 caaaacgccc atcttttaat gcgccgatgt tgatgaccat gtcaatttca tctgcaccat
    21721 tttgaattgc atcttctgtt tcaaatactt tcgttgcagt tgtcgacgca cctaatggga
    21781 atcctattac cgtacaaact agcacctctg aatcagctag tcgctctgct gcatatttaa
    21841 catgcgttgg attcacacat acagatttaa aattgtatgc tttcgcttca tcgatgattt
    21901 gatcgatttg cgtacgtgtt gactcaggct tcaataaagt gtgatctata tatttctcaa
    21961 atttcatact tactactcct cgtgttatat aatctcttta tttaatttta ctataaatac
    22021 gaatatatct cgcgaattta tatctattta tttttcagat tgtgtccaac gatttaagat
    22081 gacgaataaa actaatccga caatactcat taaacctaaa ataattaaaa taataccgaa
    22141 atgtgaactt aatgcatcat tgcctgggaa atttaatgct ttaaaatcga ttaaagccgc
    22201 agcaatcgca atacctacag ataccgccac attaataatt aaattataaa aaccaatagc
    22261 cacacctgtc atattaagat ctattgtttt aatggcttcg ttaagtaaag gtgcatacat
    22321 taaagcaaag ctacctgcaa agaatatcat agaaatgacg aagattgaaa tgtgattacc
    22381 tactgcaaat gcaggtaaaa tcaagctcag tgcgattaaa ataattgctg tgataatcgc
    22441 ttgttttgaa ttaagatatt cgccgatttt accacttagt gcaccaacga tgactgctac
    22501 aatataaccc ggtactaata gcagtgatgt tgtgtctagc tgaagatgat aaatttgttc
    22561 cattatgaat gggaacgtaa aaatataacc caattggatg gcatacatta caaatactat
    22621 aaataaaaat gaagcataac gtttattttg gaaaaatgat ttgtttacta atggacgttg
    22681 cgcattttta atatatagcg caaaaacgat aatcgcaatt aaggcaccaa tcatatataa
    22741 ccaattaaag ttcgtaataa acagcataac tgttgtagcg atagttgcca ctagaattaa
    22801 gccaacaaaa tctaaatgag ctttatttgt attattttct tttggtaata atttaaatag
    22861 gaatggcaac gtaaatacta cgattaatgc aattaaaaac atatttgtcc agtgtaaata
    22921 cgtagaaata aatccacctg ataatgtacc gataactaat gacaaggaat aactgctcgt
    22981 acttaagcca aggtaagtct tctggtcctc tttagaaaga tactttgcaa catatatcac
    23041 atataatgtc tctgcagcag ctaaacctgc agtttgaata atacgtccaa ctaaaagtaa
    23101 tgcgaattga tgttggaaaa tgtaaccaat aattgatccg ataatgatta atatcacgcc
    23161 ataaataaat agtgtgcgta tactaatggc atcagataat gaagcgtata ctacagcgcc
    23221 aataccaatt actaaaccgg ctaatgtcgc ttgccaactt actgtcgtta ctgaaatgtt
    23281 taagtcatca gcaatggcta cagaaatcaa tttgaatgag ttgtctataa ccaaactgaa
    23341 tacaaataag aataataaaa taggtactgc tttctttatt ttagaatatt caacattcat
    23401 aatcatctac accaatgaca gtgcaatttc aatcatatct gtaaatgcac gttccctttc
    23461 ctcaggtgtt gttgatgttt catgaattaa atgatcgctc actgtgaaca cacctaatgc
    23521 ttcgacacca gcgtaaattg cattcatgta taatgcagct gattccattt ctacacctaa
    23581 aatacccata cgcatccaac gttcactcgc tgttgtatcc gcgttataga aaatatcact
    23641 tgataacaca ttacctacat gatgacgtac acctttgtca cgtgctgttt caacagcttt
    23701 ttctaataac tgataagaag caattggcgc aaaatgacct ggtaattgat attgttgaac
    23761 gtaatttgaa tcagtagagg caccttgtgc aacaatcaca tcatataaat caatgttttc
    23821 ttgcatcgcg ccacaagaac caacgcgaat taatttttta caaccaaatg tatgaattaa
    23881 ttcataagag taaatgccaa tagatggcat acccattcct gaacccatga cagaaacttt
    23941 tttaccttta tatgttccgg taaaaccaaa catatttcgc actgtattga actgttccac
    24001 atcgtccaaa tatgtttctg caatgaactt agctcttaac ggatctcctg gcaatagaac
    24061 cgtttctgca atttcgacgt cattcattgg tttaatgtgt ggtgttgatt tcataaaata
    24121 ttcctcccaa tatttgtata gtaatttgtc tatttgtcta accatgtata taataatatt
    24181 ttttaaaagc gctttcaagc attacatttt gacaatatta agaacatttt acagttatct
    24241 ctactgatct tgcataaaac caatgttcaa gttgtttcta tgtttaattt ttataatttt
    24301 tccaattcga gtaaaaatta ttgtatgatg agaatgatta agtaacttta tggaggttgt
    24361 ctacgtgacg caaacacaac caaaattttt aacgatttat aatacgttat ataaagaaat
    24421 tcaaatagga aaatatccga gtggccatgc attacctact gaaaaggaac tttgtgcgcg
    24481 ctttgatgtc agtcgtatga ctttgcgcca agctataaaa ttgttagccg aagatggcat
    24541 cgtagagagt acaagaggga agggacactt tgttattcca caaacaaatg cccaacacgc
    24601 ttcaagtatg gatcaattta agcatccact tgatcaaata ttattagatc gtgtatctat
    24661 gtcatcaatt aattatcgtg ttgatttaga aagtgaatat acaaatcatt tgtttgcaaa
    24721 ccatccttct gccgtcattg caatggaacg ctattatcaa aaaaaggaca accattctaa
    24781 gcaagctgat gcattttgtt ttacctttat accgttaaat gtcattgaca catttaaagt
    24841 gaatacacaa aacgaagacg acatgaagac gttcgttgag caaacgattt atagcaatgc
    24901 atatcaatca gatttaaaaa tgtctattac caaagcgccc cacttcaaaa atcatagtca
    24961 cgtgtttgat ggtgatacac attgttggct gattattgaa acattgtacg cacaaacgcc
    25021 ctatccgatt atgattaata agtggtatat tccacaggaa atatctgaat taacactgac
    25081 acgtataaga caatcggatt attaaaatta ttgaaaaaaa atcatgtgat gactgtttag
    25141 ctattgttta atcgacaata actttcaggt aatcacatga tttttctact ttaaaacgta
    25201 tgatgtttat ttatttgttg aacgttgtct aaatgctaag aataacccag atactaatag
    25261 tagtgcggca atacttgcag taagaatact tgatgtcata ctttcaccag cagctggtaa
    25321 aacttttttg ttactttggt tcatttgatg gtttgatgat tgcatatgac cttgttgcat
    25381 gtgtggctgt tggttcatgt gtgtgttctg attcatatga cctgttttat tttgcaccat
    25441 ttcactacca ccatgattaa caccatttgc atctacattc gtttgataaa aattgccatc
    25501 ttttgttaca ggtaacgcat ggccattcat atctctaaca gcatgtggtt taaatacccc
    25561 tacattagtt gtaattatca cattgtattt atcagcaata ttttgcgtcg catcatacaa
    25621 tttcatatta tagtcttgca tattactgcc agcgcttaat tggggtaaag ctttctctaa
    25681 ttcagtatat tgtgcttgcg tcattccttg tggtactact ccgccataag ggcgatgatt
    25741 ttgtacattt tggctttgtt tattcgacac attcattgaa gttgtgtcag catgcgcatt
    25801 ttgaaaaggc atacttgaga atactaaagt gcttgttaca attaaagaac ctactgttgc
    25861 taattttttc attgtctttt ccccttatat tacaatttga ttacatttat attatcatat
    25921 gattacaaaa gaaatgcaac aaaatttttg aatcattaca attttttata aaaatttcac
    25981 tttagattca caataattac ttattttgtc aatttattta atgtcaatat gttgattaat
    26041 caatagtgtt gtctgatgta tatagcattt aagtcatcgt tatagtcaac aataataagg
    26101 tatttcgagt tgaaatttat cttattattt tgccactttt acgcgctatc ccattacaca
    26161 aaaacgaata agtaaagata ttaagtacaa aaaaggtgaa acacctaata gatgctccac
    26221 ctcaattata ttaagttata ttattttgct gcttggtata attcatcaac ttttttccag
    26281 ttaacaatat tccaaaatgc agtcatatag tctggacgtt tattttgata tttcagatag
    26341 taggcatgct cccaaacatc aaatagtaag attggtgttt tgccttctgt taatggatta
    26401 tcttggtttg gcgttgtcac aatttctaat tttccatcat taacaactaa ccaagtccaa
    26461 cctgatccaa ataatattgt tgctttattt gcaaattcat ttttaaattc atctaaagtg
    26521 ccccactgcg ctttaatgtc gtctattacg ccaccttttt cttcagaatt aggtgatagt
    26581 atttcccaga ataatgaatg gttaaaatga ccaccgccat tattacggac tgacatcctc
    26641 atcgcttccg gtaccttgtc taagttagca atcatatctg ctagtgattg atgctctaac
    26701 tctgttcctt caactgttgc gtttaatttc gtcacgtacg tattgtgatg tttgtcgtga
    26761 tgaaactcca ttgttctttg atctatatat ggttccaatg catcatatgc atatggtaaa
    26821 tttggtaatt taaatgccat aagtatattc ctccttttat gaatatactt ttataataat
    26881 taatttcggg tgtgttttgc aataattatt atttatttgt tatatccaat tatataaaat
    26941 ttaaatattc gaaattgata taatatttaa tattgaatgc aaaaaggcat ttaactgctt
    27001 ttgtttcccg cttttaaaga gaattaaaaa agacaacttc catttttcaa taagaaattg
    27061 ccttttcttc ttattcttaa atttctaaag tatagcgtta ccgatactta aattgtttta
    27121 tgtcgaagat gcctcaatat ttgccatacg tattgatttt tcatagtcat cattaataaa
    27181 agataaacta ctataccaat cgcaattaat agcagcgtag cgtacattgt aggaggcaaa
    27241 taatgattga ccacaccaag cacaataaac atcataacgg cagcaatgac acattgaatc
    27301 gtacttacaa tattcaactt cacattgatt ttagtaatat caataaatcg ccaaatgagc
    27361 aagaaaaact ctgtaattaa acgcgcaata gcagcaccgt aaattccata aaaatatatc
    27421 aaaacaagac ataatactaa attcattacc gcaccaatag tgattgacgc attatataat
    27481 cttattttat tcactattaa taaatattgc ctgcttatca acatatttaa aggaatgatt
    27541 aatacaagta tcgctaaaat ggtcatcaat gggacagttg atgcgaattc ctcaccaaag
    27601 aaccataaat aaaatgatgg cataattgca atcaaaccaa agaccatagg tattgttaat
    27661 atcaattgaa tattcatatt attagctaac gttttagtta aactatgtga ttgctggata
    27721 cacattttag taatacgcgg aatcattaca agatcaaatg tattaatcat tataattgcg
    27781 accgttaaaa tattaaatgc gttagaaaag ataccaactt gttggtatgt gcctactaaa
    27841 ccaagaacaa cgcaagaaat actagtgtat aagttgagtt gaccatttgg taataaatat
    27901 gctaatgacg aacgaaacaa ttgccagacg tgtatccaat taaccgaaac aaagctaatg
    27961 tatcgtttta aatagataaa caaaggtaat tggtttaata ctgtcacaat agcaatagta
    28021 aatacataca atgataaatc tgattgatct ttcacaaaga taacaacgac acttaataca
    28081 ataacagacg caacaatatt actaaggcta ggtattttaa acttttcagt tccagcataa
    28141 aaccatgaaa tatcgagttc tgcacctata atatagattc cttgtagtag gaaaataaga
    28201 tagtaatcat ctataaatat agtaattacg accatataca tcgcaaaaac tgttaacgct
    28261 aaaaataatt tactgacaaa gatatcccaa aactgctgag acaattgccg tttgtcgtta
    28321 acggacttcg cgataactct attaaaatat aactgaacgc caacacttgc aatcatcaaa
    28381 aagtattgca cgatattgaa agaaaatgaa acaataccca caccactggg accaaatgca
    28441 cgtgaaataa tgggtattgt aatcagcggt gtcatcgttc taatcaattg gtatagccct
    28501 tgataaataa tattttcttt tagtgaatca ctcttcatta cgatcccttt ccatcttttc
    28561 atattgaaca ataaagacga taattcctaa aataaaagcg acatatcttg agttattaaa
    28621 cgatactgtt aaaaagtaaa tcagcatcgt caacattaca acaattgctg ttacattttt
    28681 cccagaaata ttgaatctaa ataattcaaa taataaataa atcataaata tgataaataa
    28741 cgcaccgaat aaaatgcccc attccgcaaa aatttgcaaa tatgtattat gggcaacaag
    28801 taaaatacca ttaatttgcg agccaatatg tacataatcc actaagccaa caccaaaacc
    28861 taatgtatat ttaattactg aaatggcatt tatccataca acacttcgct cagatccact
    28921 atcatttaat gatgcaaacc cctcttcaaa aatagacgcc attcgatcta atgacggtaa
    28981 ggcatcaagg tcgcttaatt ggaataaata gtagtttata ttataaaagg taaaacaaag
    29041 tagaattaac ataatcacta acatactcac aacacttact gcatttctac taaataactt
    29101 tttaataaag aaataaatgg ctaagacgat taatatgata aacgcagtct tagaccccgt
    29161 tgtagttaaa gaccatagca aaataccaca tgcaaggacc ttgaatatgt aattatgaat
    29221 atacttgtaa gcaagtacca atgtaataat ctgtgtcatc gcgaaatagt tagggtcatt
    29281 cattaatcct tttgaacgta tttcatcaaa gtataataat ttcattagta aaggggactt
    29341 gttcaaacca gctataatac aaaatagacc tatagtcaca ctgcttataa tataagaggt
    29401 aacgatcact tttttgctat tacctaactt aaagatgatc atccctaaat agaaataaat
    29461 gactacaaat gcatatttaa ctgtagatgc aagaacttcc ttaaccgtaa taaatatcaa
    29521 atcatcaaaa aatagtaaac aaagcgtaat aatcatacga tatgtataca aaataatgaa
    29581 aaactgtaaa aaatgatttg cctttaataa atggttggcg aaaaacagta aataaactaa
    29641 tattagtaat gtgataaagt cagctataga aacattcaca ccggcaataa ccgaagattg
    29701 ctgaataaaa accgctaaac cgataagtaa caatgttaga tatttactat tgtgttgatt
    29761 ttccattata aacgtcttcc acttctttaa tcattttctc ctctgtaaaa cattctaaat
    29821 aacgttttct agattggtta ctcattttga tgtaatcact gtctattaaa tatttttcca
    29881 ggactttggc aatagtttca ggttgattgt tcactataca tataccatta tcagttacta
    29941 attccgaaat gccaccaaca tgactagcga tgacaggcaa ccctgtagcc atcgcttcga
    30001 taatgctaat tggcaaacct tcatgcttac ttattaaaat aaacgtatcg tattgtgata
    30061 ataaatgact cgcattaatg acattgccca aaaatgtgac atcattttct aacccagctt
    30121 gtacaacttg ttgctgacaa tcatttaatg taggtccatc gcctataaat gtaaaatgcg
    30181 catgattact gttatgtaat ttcaatatct ctattgccgc gattagattt tgtggcaatt
    30241 ttggataagc aaatcttgca atcataacaa attgatgttt tgtcgagtca ttaatctgta
    30301 aatcttgttt attaggcaac attccaacaa cttcgccaat attgttatgt gattggcttt
    30361 ttagcgtttg cttaacagcg ggaacatctg caattccatt atgtatagtg gttaatttca
    30421 atcgattaaa tcgatatttt aacgctaact gtttatcgaa atctgaaaca caaataatgc
    30481 tatctgtaat acgtgacatt aatttttcaa taactagata tagaaatttt ttagctggtt
    30541 taacaccctc tgtaaaagcc catccatgcg cagtaaaaac tacacgtgtg tctttcgatt
    30601 tcgaaatgaa cttcgcaatt cgtccgaccg ttccagcttt ggaagaatgt agatggataa
    30661 catcaggttt aattttcgag aataactgtg ctaacacttt gacagctaaa atatcttgtt
    30721 taaagtcaat tggacttact aaatgttcga taataattac attgactctt gcatctagtt
    30781 gttcaatcat tggtccatga ttgcctacaa tgacataaac atcatggtgt acgcaaaaat
    30841 ggttagcgag ttgaatgaga tgtgtttgtg caccaccatt atctgcttta gtaatacaat
    30901 atataatttt caactgttac aaaccccttt aatgctatac tttcaatttc ttaacatggc
    30961 tatctcatca gatgaatagt atttatagcc atgcaaatca atgatggcaa atatttctta
    31021 atgccatttg atactgtctc aagggattcc tcgttatact gtaacaattg gtcacaatct
    31081 ttaaaatgta acttttattt aaacttatta agcaaattaa gactaccttg agccttcccc
    31141 tgtaataaca accatcaatg ttctaattga tatatataat tccatcatta agctaccttt
    31201 atgtatatat ttcatgtcat attttaattt ttgttgtggt gttaagtcat atccaccttg
    31261 aatttgcgca agacctgtta atcctggtgt aacaagacat ctttgctcaa atcctattat
    31321 ttctgaacta aataattcta caaattctgg acgttccggg cgtggtccaa taaaactcat
    31381 ttccccttta acaacattaa ttagttgtgg taattcatca atgcgtgttt tacgaataaa
    31441 cttcccgaca tttgttatac gatcatcatc tttatcagcc cattgcgcac cgtttttctc
    31501 tgcgttttta cacatcgaac gtaatttgta tattttaatt aatttaccca tcttcccaac
    31561 tctaacctga ctataaatag ggtttcctgg cgaatctatg acgatagcaa tggcgaatat
    31621 gaacataatc ggaaatgtta aaaataataa aacgatactt aataccaagt caatcgcacg
    31681 tttgattgga aaatagccct tttttacttc ttctagtgtg tctaattttc tttgataggc
    31741 gtaaccctta ttacggacag cttcaatgcc aatttcacga catttccact tctttcgctg
    31801 taacttcttc aatattatct acctcaaaat taaagtaatc ctttaagcct gtttccacag
    31861 tgtatttagg aacaaatcct aatgctttta aattagaaat atctgcataa gaatgcttaa
    31921 tatctccctt tcttgcttct ttaaaatcat gctcgactga ttttccatat aattcaccaa
    31981 taatgcgata aacctctagc aaattcgtaa atgtacccgt accaatgtta taaccatgtc
    32041 caaccgcatc tttatgctcc ataattaagc gcacagattg aacaacatca taaacataaa
    32101 caaagtctct cgtttgcaga ccatcgccaa aaaatgtaaa aggcttgtta tgttcaaatg
    32161 aatcgaacat ttttgaaatc acacctgaat attgagactt aggatcttgc cttggcccaa
    32221 atacattaaa gaatttaaca acagctgttg gaatgttata tagcgagcaa taatttaatg
    32281 tcgtacgttc gccgtaatat ttatctattg catatggtga taatggtaga attaatgatt
    32341 gatcactttt aggtaaatca ggtaaatcac cataaacggc tgcagatgat gcaaagatga
    32401 atcgttttat atgactgtta tattttttaa tggtttctaa taatttcaac gttgctacga
    32461 cgtttatttc ttgagataag ataggtttct caaccgactc agcgacacta actaatgctg
    32521 ctaaatgaat aacataatca aattgatatg tcttcatgat ttgttcaact gcatcatatt
    32581 cacgaatatc taattcaaac acatgatcgt cagccaaact tttaatattt tctcgtttac
    32641 ctgttctata gttatctaga acataaacat cataatcttg ttgcaaatca tctactaaat
    32701 gtgacccaat aaaaccagcc ccaccagtta tcaaaacttt ttccaaatct tccacctcat
    32761 ttatacatta aaaatatatc acaaaaacat aaagtattgt aagcttttta tcggtatttt
    32821 ttatttataa aaataaaatg cgataacttt gtgaattttt atgtagataa attagatagt
    32881 ggtgttttat gatgttttag cgtaactatg cttgatagaa tttagtaatg ctttcaactg
    32941 atgcattaga ctgtgacatc ataactcact taagagcttc tgttattaat tttctactaa
    33001 tacaatccct tctaagtgca ctaaaaaatc cttactgcta agtgattaaa cttaacaata
    33061 aggatttatt tattattaat ggatgattat taacggaatc tcataccacc atctacaata
    33121 attgtttgtc cagtaatgta atcagagtct ttaccagcta agaagctcac tacatttgaa
    33181 acatcttcgg gttgtgaaac tctgcccaaa gcaatctgac ttgtaaattg ttcccaaccc
    33241 catgcttcag gtttacccgc ttcttcggct gttgccactg cgatactttc catcattggt
    33301 gtttgaacga taccaggtgc gaaggcattc acagtaatac cttcagacgc taaatcttgt
    33361 gcggctactt gtgttaaacc tcgcactgcg aattttgtac tgcaatataa agacaagcct
    33421 gggttaccct caacgcctgc ttgagatgtt gcattgataa ttttaccgcc atgattgaat
    33481 tttttaaatt gttcatgtgc ggcttgaata ccccatagca cacctgcaac gttcacgcca
    33541 tatactgttt taaactgttc ttcagtaatt gtatcgattg gtgttgttgg tccaaggccg
    33601 gcattgttaa ccatgacatg gaaatcgcca aattgcgctg cagtttgtct tactgcatta
    33661 aatacatcat cacggtttga tacatctgct ttgatagcaa tagcttttgt accatcactt
    33721 gataatttaa gtgcagctgc ttttgcccct tcttcattga aatcaacaac tgctactttg
    33781 aaaccatctt ccactaaacg ttctgcaatt ttaaaaccaa tcccttgtgc tccgccagtt
    33841 actaatgcta ctttgttgtt tgtcataaag atcactcctc aaattacttt cctttaatta
    33901 cattttactc ctcttcattt gaatagtaca acaaaggtaa ctccatttaa caaaatattc
    33961 agatatttaa ggtatagtta aacgcactac ctttagtgat tggcaatgcg tttaaatgtc
    34021 gttttaaaag tgtttatgtt gaatattatt ttttaagtct ctcgattagt ttgtcatcaa
    34081 tcttttttcg agacatggtc ttttgattca atcggcggtt ccgtattatc actgtcaact
    34141 ttagttgtag cttcatcttt atgtacttct tcgtttaatc cttctaggtt tttagtcgtg
    34201 ggatttttaa actcaggatg ttccatcatg tcttgactat caagttcctt tttacacgtg
    34261 tctttatgtg atgcttgatt tgcgttccct ttactttttt gaatagtggt agtatctgat
    34321 gtagatacta atttttttct acctaaaata gatatggctg aaacaaacca gagtattgca
    34381 gatacaaagt tgcataatac taaagcgata atagccaata caattaatat gacaccattt
    34441 gaaatccttt ctttaaataa gtcagatgcc aatacgatga caggtacgat tgaaagtata
    34501 attacaaata tagaaattat tgccgatata actatcgtta ctattaaata atcagctctg
    34561 ctacctgaca ataaatagaa aaggccgaaa attagtccat agcaaattac aaacccacat
    34621 aaagttatag ccatgagtac tatataagct atttgaaaat ataaacctat ctttatgaat
    34681 gatttttcta catttttttc catgtctatt ccccatttat ttaatattta tactttacct
    34741 taatatacct tattttattt aatttttata tgcaaaatac aaaaatggag aacttcaata
    34801 tttataaaat atcaaaagtt ctccacacta tattgtttta ttatattttc gctattaata
    34861 ggctaaatca tcatatttcc ctcaacatca cagtaaaact attgctccac caagtatact
    34921 ttttcagtat aacatctcat attggcaaac ttatctgcta aaaactgctt ccaattgcgc
    34981 agttgttcaa catcatcatc ttgtttaagt aatgccagtg gtacttgaag attaagacat
    35041 cgtcctgaaa tattaaagcg tgtcacacct gctggcacag tttccccttt atgaacaacc
    35101 gcttcaattt ccttataact caatggctga tacttcatga gtacatcttg ttgagaaaga
    35161 caaggatatg taccttgtgc aattctctct acagaacaac aaccactata acttgcgaca
    35221 accttttccc atacttgaaa atgtgcttcg cctaaatctt ttgtatacaa atattgttct
    35281 gtatcaccat gacacatcgt aataaatggc gcttcttgtc ttgtctcagt agtccatggt
    35341 aagcgatgtt cttgttgtaa cgtttcccac cacacaccaa atggaacttt atgttgccat
    35401 gtactaattg aatattgtgt ttcatggatt tcttgcactg gaactttctt acatcctaac
    35461 gctttcaaac ttgtataccg atgcacacca tctataacca tatatctacc atgttgcatc
    35521 gctgtcacta aaataggatg acgtataaaa tcatctgctt caatactact tttcgttttt
    35581 tccaatctta aaggttcgaa tgtttcgtga agatcaatct tatctactgg taccaatttt
    35641 aaatgttcat gaatatgatt caatagttat tcatcctcct ttgtttgcgt taaataaata
    35701 aattcaggat gtggatggct taagaaatcg tgatgtgaaa tagaccatcc gtatgcacct
    35761 gcatatttga aaacaataac gtcgcctgta ctgattgcct ctatctgtat ttctctagca
    35821 aagacatctt tcggtgtaca taattgaccg actaacgttg tgtcctgtct cgaaattgaa
    35881 actttttcaa atgaatatgg attgtcctta tagcgataaa tgtcaaaagg atggttatgc
    35941 tgccaagata ccggcagtct aaattgttgc gtacctcctc ttaaaatggc ataccaagca
    36001 ccatgtactt tcttaatgtc tagcacttct gtcacatagt aaccaatatg tgccacaata
    36061 aagcgcccac attcaaagtt caatgtcaca tcttccattt cttgctcaac gataagtgtt
    36121 ttaaaatttt ctacaaaatt atcccactca aattggctag ttaaatctgc atagttaacg
    36181 cctatgccac caccaagatt gatatgtttg agaggaaatt gatgtttttc agaccatgcc
    36241 tttgcttttt taaaataaag tttcactaca tcgacatgta aattcgagtc taaattgtta
    36301 gaaatagaat gaaaatgaaa tccatctaga tgaatatttg gcattgctag cgcagcttca
    36361 atgacatcat caacttcgtc ttcagaaata ccaaattgtg ttgggcgtcc tgccatatgc
    36421 aacgttgcat tgggaaatgg tcctgctaaa ttaacacgca ataaaatgtg ttgtgtctta
    36481 tcttcatctt ctaagatggc atttagccgt tgtaattcat gcatactttc aacatgaata
    36541 cgctgaacac cttcacttac tgcatatctt agttcctcgt ctgtcttacc agggccacca
    36601 aaaataatat gatttgctgg tttaaaaaca agaccttttg ctatttcacc ttgagatgca
    36661 acttcgaatc cttcaacata ctgactaatt gtatctagga ttgttcgttc actatttgct
    36721 ttcattgcat agtacatctg acaattacta ggcaatgacg atgtaatatg ttgcaaatga
    36781 tgttccagtc cgactaaatc atagatataa tgacaaactg gatgagattg tgcttttaac
    36841 tgttcaataa caggttgaac tatacgcatt agtcttcatc ccccttctgt ttagacgtcg
    36901 ctagagatgc acttaaatgg cgatatattt ttccgcgatc atcacctaaa ataaatgttt
    36961 gtacaccttg tgcctgccat tttgcaatat cttcatcttc acgtggtaat gcacaaaaat
    37021 gtttaccatg tgcattcaca acttcaaaaa tatgttgaac atgtgatgtt acttgatcat
    37081 cacgcgtttg ccatggtatg ccaagtgact gggataaatc tgcggcacct tcgactatca
    37141 tgtctaaacc ttcgacttgt gctatatcgt caatggccat aaccccttca acatcttcta
    37201 tcatggcaat caccataata tgctcattag ccatctccat tgcatcaagt aatggtgtac
    37261 gtccaaatct tgccatgcga ccaccattca aacttcttaa tccttgcggg taataacgac
    37321 ttaatttcac aatatgctca actgtctcac gatctttaac gtgtggcaca ataatacctc
    37381 tcgcacccat atccaacact ttaatgatat ctctatctat cactgcagtg acacgtacaa
    37441 ttggtataat atgcgctgct tcagctgcac gaattaaatg cgctagtgtc tcatcattaa
    37501 tcgccacgtg ttctgtatca atcacaacaa agtcataccc gcttgctgcg ataacctcta
    37561 tcatcaatgg gtccggtata gaattaaaaa tgccataaac tgaatcacca ttgtttaatc
    37621 tatgtttcag agatagttgt tgcatcagta atacctccta cacctaatgg atttgtaaca
    37681 tgatgaattc ttaactcgga gtcacttaat aatcgacgtg tcgttaactt ttcaacttga
    37741 atcgtaggtt caaacaaatc aaaatgttga tagttattca gctctggaaa tgcttcttga
    37801 tacgcctcaa tgatgccttt aacccatttc cattgcagct tctcatcgat accatattgc
    37861 ttttcaataa ataagatgat ttcggcgata ttaataaaga aaaatgcatc atgtaaaaag
    37921 tcacgtacta aacgttcatc atctgtttca ataaatgaat tactattcac ttttttatgt
    37981 gcttctggca ttggctttaa tgtcaggtgt gaagcagctt cacttaaatg ctcacgctta
    38041 aaacgaacac catcatggaa atcttttaag gcaatacgtg taggccaacc attttcatga
    38101 atgagcatca tattttgtgc atgcgattca aaggcaatac cgtgataata aagcatatga
    38161 atcattggac gaatcgctac agctaaaaat tgctttgtcc aagcttcaga accatattgt
    38221 ttaatccaat tttcaatgaa tggtacacca tccttatcac tcgcataaag tgcattaaat
    38281 ggtatcgcat cctcttcatc gattaacata tgatatatat tttcacgcca tataacacct
    38341 aacgcaccat aaacttgagt ttgtttataa ggcgaaagtt gtgtatttaa ataagactgt
    38401 cctaagactt cccctagaaa agctgtcttt aattcatctt ttaaatacgt atcttgttgc
    38461 tgtatctgct ttaaccaatc cgtaatttgc gctgcatttt caattgtatg aggtgccaac
    38521 actcgtttcg ttgaagtatt cgttatactt attggtacct ttaaataata ttttgtcgtg
    38581 tctattggcg acatcgtacg aatcgattgt tgagggtgat atagctcatc actttcccct
    38641 aaccatagta ctgtgccatt aagcctttct tcagccaaat caacttgaat gacatgttca
    38701 aactgccatg ggtgtacagg tatcatctca acatcattta catgtttgcc agacgcttca
    38761 atttgctgta caaaatgttc ataagtctta tcgccaactt gttgacgtaa catttcgtta
    38821 actacaacat ttcttgatac cgtcgtttct actttatctt tgtcgatagc taaccactgc
    38881 agtttaacgt ttggtacaaa gtcaggacca aatttcaaat tatcactcaa cgtaaatcct
    38941 aaacgtgatt tgtaacttgg atgatactga tgcccttcca tcgcataaaa ttcatagtcg
    39001 ttaaatgtct caggtgttgc tggtgggttt gattctcgat actgcatact ttgcgtatct
    39061 tttagttctg tctgtaataa ctcgacaata aattgttcta gcttttcatc atttttagga
    39121 aatgtaaata caacctctct cagtaattgt gtatagtctg ttgttgtatc tgcctcatct
    39181 cctacgacac gctcaattgg tgatgtgata cgtatacgat caaagctatg tgtcttttca
    39241 gcagtaaaac gatactctga atcatttcct tctattgtaa aataaccgac accatcttga
    39301 tatgacgctt tatacacaac aatattctca taaataagcg atgataccaa ttggtacatc
    39361 actctagtct ttacacgatt aagaattgtt tgattcacaa tacgatacct ccttgttatg
    39421 acaaattgga tttggtatat gtgtataaat agggtttgca ccacaatcat tcaatttact
    39481 catcaaattc gctttagccg caatggtcgg cgtttgatat aaatcttcta cacagtcaat
    39541 aaatactgcg ttattcgcat attctttttt ccaagtcata agacgatgcg ctacaagttt
    39601 ccataacaca acttcatttc tagtcgcttt accaatagtt gatactaaat gtcctaagtg
    39661 atttactaca acgtaatatt taagacgatg ccacgcttca tcatgtgcat atacaacagg
    39721 gctagatgct gcaacaacat ttggcacaag ctgtttttca gtagcaatcg ttctagatag
    39781 acaaatacct tctaaatctc tgacatagca cacttctggt atgccatctt ttaattcaat
    39841 taatgtattt tgtacatgtg cttctagact aatgcctgtg ttactaaaca gctttaatat
    39901 cggcaataat gtacgatcca aataacattc aagccatgct tctggtgcta aaccactttg
    39961 ctcaatcact tgtgataact tagaggtcgg tgaatcaggc atcgtttcaa ataatgacgc
    40021 caatacatga atatctttat cagcatggta attcggtatc ccttcacgaa caatcatggc
    40081 actatttgtt aataaatcca tttcaggttc aactgtttgc cctaatggat tcggtaacaa
    40141 tgcacgatat ccttcttcaa acatcaattt aaaatggggt gtttcaacct catctttgac
    40201 tgatgcgata acttgcgcgg catcaattgt ccgttcaatc tgttcaaggt cattcgtacg
    40261 tataaaatta gtgattttaa cgtgtatcgg taatttcaaa taaatgttta atgcttttga
    40321 aaatacagtt ctaaccgaag acgtcgggta cactgaatca ccggaaacgc caaggtcttt
    40381 tatcaaacct tgttcactat attgcatata ctgtggatgc tgtcgcaaca cattgatttg
    40441 ataaggatgt attggtaata aaataaaatc tttgggtatc tctgatatgt ctatatctgc
    40501 taattgatac aacactttct caacctgatc ttctttacct tctacatagc gcgtgagcag
    40561 aacatcttga tgcacagcta aataatgcaa ttggaatgat gtatgacatt cgggtgcata
    40621 tttctctaaa tctgcttctg aaaacccact tgcactctta ggagtcggat gaaatggatg
    40681 acctaaatat aaagattgtt ctgaaacgat atagcgatcc tctgcgtagt ctattgtgtt
    40741 actttgcaaa taacgcgccg tgcgatgaat gctattatcg atgtcagaca taatttgcgc
    40801 catatgttgt tgcactgccg tttgattatc tgcactttga gccatatgtt gcaaaatacg
    40861 cgcaatggct tctttatatg ttgttaattt cttactttta ccatcaataa gccatacttc
    40921 tggatgatac atatgatgcc ccatcgcaga ccaataagcg aattgacctg ttaacgtttc
    40981 gagctcagat aattgtatag accattgatg attttgaggt ggtacttgat ataaattttc
    41041 ttctctaaaa tattcattta aaatacgttc gatagccgca tgcgctgcat gttgtattaa
    41101 ttctttattt tgcacttttt tgtttcaact cccataattt cattaatgtg tgatcgttga
    41161 tttgattagt gatggttgaa caaattaaaa ataaactact tacagcaaat actatgccca
    41221 taacgataaa cgtagtagct ggtgtagtat aacttgtaat ggcagcgcca ctaagactac
    41281 caataatttg accaataact aacatactat ttgtcgtacc aacaaatgtg cctttaagtt
    41341 gttgatgaca cgcattcacg acgacaaaca tgacactttg aatcaatgca ctatatgtta
    41401 atccttgaag tattcttgca gccattaaaa attctacatt cgtcgctaaa ccttgcagta
    41461 tcgcactaca accacatgca atcgtggcaa atatatatac tgatttaaca tatgatttat
    41521 cattaaagcg accccataaa ggcgcgctta atatcgaagc cgtccaaaat gcggactgta
    41581 aaaatccaat cacactacgg tcatctatcg ctgtatgatt cactgatgaa gcaagtggtg
    41641 ataatgcagt tagcatgcca tacatagcaa agtttgctaa aacgccaacg ataataaatc
    41701 gacatgtttg ttgtgtgcat aatagacatt gaaatgaacg gcgaatacct ttattaatat
    41761 ttggtgtttg tgattttggc atatgtgtcg tttcaatcaa ttttaatgca ccgaaaatac
    41821 agacaataaa agtaataacg gcaatactca tcagtaacgc actaaaacct aatatcgaag
    41881 ctgtaacacc gccaattaat ggtcccacaa gagaccctgc gctgactgaa ctttgcagtc
    41941 ttcctaatac ctttccacga tcttcagctg gcgcctctgc actcgcaaac gcacttgatg
    42001 catcaacaac accaccaaat agtccctgca ataacctcac aagtacaaac tgtaatggtg
    42061 tagtacacaa tgccattaaa aataaacata ccgccaaacc aagtaacgct cgtagcacca
    42121 tccattttcg actgatctta tcacctagct tcccccatat cggcgaagct atcatcgtcg
    42181 ttacagctgg agcagcaatc gctataccac tccacaactg tatttctacg actgatagat
    42241 tttgtagtga tgccatataa attggcaata atggcacaag tactgtcagt ccagcaatcg
    42301 ctataaactg actgagccat aaaatgcgaa agttactgcg ccatatagac tgattaatca
    42361 tatgtcacca ttggatttgg tacggtagtt aaacctgaag gcatactacc tccaccacta
    42421 tcacgttgat atagcaatgg taataaaatt tgtttaaatg gccacgtctg tttatcaaat
    42481 aaaatatgtc tgacagctag ctgatcagtt gtaacccagg aaatagttgc cacttcattt
    42541 tttaaaattt gttttaacaa cgacataagt tcatgctcac ttacaccaaa taaatcttga
    42601 attgcatcaa taatggcata tagatttacc gatacagcta atgtttgaaa ataagcaaag
    42661 aatgtttcca aatcttcatt aattagcgta ttaggtgtat cttctctgac gacatacttc
    42721 ggcaatgaaa gctgatgtgc tgttagccat ggtttataaa ttctgacagt atcatgatca
    42781 cgtaacacgc atttttgtac acgtccatct tcaaatgaca acaatatatt ttgaccatgc
    42841 aactctggta atgcgccgta ttgcataaat gatagtgtta cctttaaaaa gacttgtgcg
    42901 atatcttcaa ataacgtcat gacatctttt ttagaaatat tatcttttcc acaaatcatt
    42961 tgatataagg tgcgatcatt tgccgcgagt gctgccattg acactagctg ttgcgtatca
    43021 tttttggcta gcacttcggg atattttctt agctgaacag ttaaatgacc taattgatct
    43081 ttgaaaatat cattatcttg acccatatat gaccaccaag ctgtttcatc acaaactgtg
    43141 acatacttag ctaacatggc atctttttct ataagttgac gtaataatcg ttcggcttgt
    43201 tctccgtttt tcatgtaacg cgtaggcgtt agccttaatg cgcctaatga ctgcattgca
    43261 aatggcactt taacatggtt atacggcgct gcgatattaa ttaatgaacg catacttgaa
    43321 gccgatagat aatctccaaa ttttaatggt aatggtacaa ccaatttttc cctaatctct
    43381 ttcgcaaaga cgttcggcaa aatatgttga tattgccaag gatgtacagg aaatagtaca
    43441 tagtcatcta ttgataaccc ttgatcatgt aacatatctg tcgcttgttc ttttataggt
    43501 gctgtcaatg attctaattc atcaatattt gcagtatcgc catgaatcat ttgtgtcttt
    43561 ttaactgctg caaccattaa aggaaatgat tgatttaatt cagcttgata cacttgataa
    43621 tccgcttctc ttaatcctct tttttcttta gctaatggat gaaatggacg atcttttaaa
    43681 cttgcaaact gttcggacat cacaaaagga tgcgacgcta aatctaattc tgataattgt
    43741 ttagcaagct gtgtggcagc agtagtcagt ccttcttcaa cgcgagccac ttcccattca
    43801 tgacttaaat cacaattcat attagcgatt gtttgccaaa attcagctgc cgttaaaggt
    43861 tgcttagacg cccttccctc tatcgtaatt ggttgtgaac tttcataacg aaacatattt
    43921 aaagcactaa aataaacagg tatctttatt tgttgtgttt cacgtttgta tatcaaaatg
    43981 gtttgtccat tttctttagt aatctcacta ttcgatacaa taccggctat atcttcaaat
    44041 aataatgcat caactaaatc ccttaatatt atcgtttgtg ctgtattgac tgctgtatga
    44101 ttctgcaatg ttcagacacc tcgcattctt aatataggtt caatgttgtc ccaatatttt
    44161 gttgttgtgc ctgttgataa ataaagtaag cacttgaaat atcttcgata gccataccca
    44221 tcggattaag taatatgatt tcatcatcgt cttcacgtcc tggtgtgtca cctgtcacaa
    44281 gttgccctag ttcagcatga agtgcttctt tgctgaattt accttctaac accaattggt
    44341 taatagtttt cttttctcga ttacattgtg accagtcatc tactacgact ttgtcagctt
    44401 taataaagac ttctttatgc acatccatga tagaaatgtt gctaataaat gcaccctttt
    44461 gtaaccaatc attttcaata tatggttgat ccgttacggt acatgtaatg actacttcac
    44521 catttgatac tgcttcttta gcattttctg tcgcaataaa attaatttcc ggacgctgtt
    44581 gttgccatct atcaacaaag cgtgcacacg cttcagagaa ttgatcgtaa acaaaaacgc
    44641 gttcaatatg atcgaattgc tctaacatac tttgtaattg cttgtctccg attagcccgc
    44701 aaccaatgat tgttaagtct ttaaatcctt ttttagccaa atgcttagct gcaatcactg
    44761 aaactgctgc agtacgcata ctactaatca aacttgcttc cataactgca attggataat
    44821 tcgtttctgg atcattcaaa ataatgacac cacttgcacg ctccatatta cgtttcgatg
    44881 gattgtcgtg cttactacct atccacttaa tacctgaaat tgcgtgttca ccaccgatat
    44941 gacttggcat tgcaataatt cgatctgcga tgtgtccatt ttcaggatcc tgtcttaaat
    45001 acggcttaag cggttgtaca aaatcattgt gcgcatgcgc tgttaatgct tctgttaatg
    45061 cgtcaacata aacttgtgaa tgatttcctc ccgcttgttc aatatctgat ctatttaaat
    45121 acaacatctc tctattcatt ctgatttaac tccttgtctt gatttcattt tttctaacca
    45181 tgtatctgaa taaactaaat ctaagtaacg atcgcctcga tctggtaaaa tcgtgacaat
    45241 tgttgcacct tcttcaattg acgttatcaa ctgctcaatc gctgcaataa tcgaacctgt
    45301 tgaacctcca gcaaatatac cttcataatc aatcagtttt cgacagccca aagcagattg
    45361 ataatcatct acatggatca cttgattaat ttctgatcta ttcaatattt cgggtacacg
    45421 actagcaccg ataccaggta attctctatt aataggtttg tcaccaaaaa tgactgaccc
    45481 tttcgcatca acagcaacaa tttgtgcgtt tggatgcact tctttaattt ttctactcat
    45541 acccataatg ctacctgtcg tgctgactgg cgcgacaaaa taatctatag gttgcttaat
    45601 tgtttcaaca atctctgtgc ctgcaccatg ataatgggat tgccaattta actcattcgc
    45661 atattgatta atccaatatg catcgtcaat agtggctaac agttcttgca cctttgcaat
    45721 acgagtcatt aaataacccc catgtgcatc aggttcttca accatttcta cattggcacc
    45781 ataactttta ataattttca aatttgttgg tgatatttta ggatcaacaa cacacgtgag
    45841 ttttaatccc ttgattttag ctatcattgc caacgcaatg cctaaattac cagaagtact
    45901 ttcaattaaa tgtgtattct cagtgattaa accatgttta ataccatgtt caatgatgta
    45961 cttggcaggt cgatctttca tgctgcctcc aggattcata tactctaact ttgcaaacac
    46021 ttcatgtttc ggaaatagtt gatgaagttg aaccataggt gtttgcccta cagaatctaa
    46081 caatgaatcg tgacatgctt gacttttttc aatcaaaagc gcttcctcct caaatttaaa
    46141 attctataat attgtgtgtt acctaattga taatgattct cactatcaag taattaggat
    46201 tatatttttt atgcatttat atgtcaaata attataagtt gcatgtaaat cataaatatt
    46261 ttattgactt aggaaaaaat ttaattcata ctaaaccgtg ataatgattc tcattgtcat
    46321 acatcacgaa ggaggctaat tagtcaatga ataaagtaat taaaatgctt gttgttacgc
    46381 ttgctttcct acttgtttta gcaggatgta gtgggaattc aaataagcaa tcatctgata
    46441 gcaaagataa ggaaacaact tcaattaaac atgcaatggg tacaactgaa attaaaggga
    46501 aaccaaagcg cgttgttaca ctatatcaag gtgccactga cgtcgctgta tctttaggtg
    46561 ttaaacctgt aggtgctgta gaatcatgga cacaaaaacc gaaattcgaa tacataaaaa
    46621 atgatttaaa agatactaag attgtaggtc aagaacctgc acctaactta gaagaaatct
    46681 ctaaattaaa accggactta attgtcgcgt caaaagttag aaatgaaaaa gtttacgatc
    46741 aattatctaa aatcgcacca acagtttcta ctgatacagt tttcaaattc aaagatacaa
    46801 ctaagttaat ggggaaagct ttagggaaag aaaaagaagc tgaagattta cttaaaaagt
    46861 acgatgataa agtagctgca ttccaaaaag atgcaaaagc aaagtataaa gatgcatggc
    46921 cattgaaagc ttcagttgtt aacttccgtg ctgatcatac aagaatttat gctggtggat
    46981 atgctggtga aatcttaaat gatttaggat tcaaacgtaa taaagactta caaaaacaag
    47041 ttgataatgg taaagatatt atccaactta catctaaaga aagcattcca ttaatgaacg
    47101 ctgatcatat ttttgtagta aaatcagatc caaatgcgaa agatgctgca ttagttaaaa
    47161 agactgaaag cgaatggact tcaagtaaag agtggaaaaa tttagacgca gttaaaaaca
    47221 accaagtatc tgatgattta gatgaaatca cttggaactt agctggcgga tataaatcat
    47281 cattaaaact tattgacgat ttatatgaaa agttaaatat tgaaaaacaa tcaaaataat
    47341 taaggagttt tacgatgcta cttaaaccaa aataccaaat cgttattgct ggtttatgtc
    47401 ttgcaatagt agctatctta agtttaatga ttggaaatac gcttgtgtca ccaggtacgg
    47461 tgatacaggc gttattcaac tttgatagtg aaaacgattt acatgatgtt gtcactggtg
    47521 cacgggcgtc gagaacaatc attgcgctat tgacaggtgc cgcactagcc gtctcaggat
    47581 tattgatgca agcacttact cgaaacccaa tagcatcgcc ggggcttttc ggtgtcaatg
    47641 caggcgcagt attttttgtc atctttagta ttacatttat ccaaattcaa tcttttaaaa
    47701 tgattgttgt catcgcgttt ttgggggcta ttgttgttac agtcttagtc gttgcactag
    47761 gtatgtttag acaaacccta ttctcacctc accgtgtcat tttagcgggt gccgcgattg
    47821 cgatgttatt tacagctttt actcaaggca ttcttattat gaatgaaaca gacttgcaag
    47881 gcctattatt ctggttaagt ggttccgttt cattacgtaa tatttgggat atcccatgga
    47941 ttattccgct tgtattgata cttattttaa ttgcgtttag catggctgca cacatcaata
    48001 ttttgatgac aagtgacgac attgctactg gactcggaca aaacataaaa ttaatcaaat
    48061 ggatgattat aatgctcatt agtatgctag caggcatctc ggttgctgta gctggatcaa
    48121 tcgtctttgt aggacttatt gtaccgaata ttagtaaacg attattacca ccaaactata
    48181 agtatttaat tccttttact gcattaaccg gcgcaatttt gatgattatc tcagacattg
    48241 ttgctcgtat tattattaag ccactagagt tgcctatcgg tgtcgttacc gctgtcattg
    48301 gcgctattgt cttaatctat cttatgaaga aaggacgtca acgcttatga ccgaaaagat
    48361 taataaaaag gacaattacc atctcatctt cgcgttaatc tttttagcca tcgtttcagt
    48421 ggtaagtatg atgagtggtt caagctttat accattacaa cgcgtactga tgtactttat
    48481 aaatccaaat gacagtatag atcaattcac tttagaagta ttacgcttac ctcgcattac
    48541 acttgcgata ttagctggtg gcgcactagg gatgagtggt ttaatgttac aaaatgtatt
    48601 gaaaaatcca attgcctcac ctgatattat cggtatcaca ggtggcgcta gcttaagtgc
    48661 tgttgtcttt attgcatttt tcagccattt aacgatacat ttacttcctc tatttgcagt
    48721 aataggtggc gcagttgcaa tggtgattct attagtgttt caaacgaaag gtcacattcg
    48781 accgacaaca ctcataatca ttggtatttc aatgcaaacg ttgtttattg cgcttgtcca
    48841 aggattactc attacaacga agcaattatc tgctgccaaa gcttatacat ggctagtcgg
    48901 aagtctttac ggtgctacgt ttaaagatac aatcattttg ggtatggtta ttttagctgt
    48961 tgtgccgttg ttatttcttg tcataccaaa aatgaaaata tctatacttg atgaccctgt
    49021 agcgattggc ttaggcttac atgtacaacg tatgaagctg attcaattaa tcacttctac
    49081 tatactcgta tctatggcaa tcagtttagt aggtaacatt gggtttgtcg gtttaatcgc
    49141 accacatatc gcgaaaacaa tcgttcgcgg aagttatgct aaaaagttac taatgtcagc
    49201 aatgattggt gccatatcaa ttgttattgc agacttaatt gggcgtacct tattcttgcc
    49261 taaagaagtg ccagcaggtg tatttattgc tgcttttggt gccccattct tcatatactt
    49321 attattaacc gtgaaaaagt tataaagata ttattaaaac aaaatgacct cacaacaacg
    49381 ttagcaaaat gattcagcta actaacagtt gcgaggtttt tttatacata tagttgttgt
    49441 taatgttaac aagcgtcgac tttcttaatt acatattaac actttatata caaataacac
    49501 cgactcatat tctataatat caatcaatat tcttcgattt ttcaaatatc gataactatt
    49561 tcttatttaa atatagtgtt tgataatgtc atttattcaa aaacacaaat tttaataaaa
    49621 atatcatatt atttttaatt gtaaattacg gattattttt cttattatat tgtgttatat
    49681 taaaataaag tgcatataca aggagataga acaatgaaat ataataacca tgacaaaatt
    49741 agagatttta taatcattga agcatatatg tttcgtttta agaaaaaagt caagcctgaa
    49801 gtcgatatga ctataaaaga atttatatta ctgacttatt tatttcatca gcaagaaaac
    49861 acacttccat ttaagaagat tgtttcagat ttatgttata aacaatcgga tttagtacag
    49921 catataaaag tacttgtgaa acattcatat attagtaaag ttcgaagtaa aattgatgag
    49981 cgtaatactt acatttcaat atctgaagaa caacgagaga aaattgcaga acgtgttaca
    50041 ttgtttgatc aaatcattaa acactttaat cttgcagatc aaagtgaatc acagatgata
    50101 ccaaaagata gtaaagaatt tctaaacttg atgatgtata caatgtattt caagaatatt
    50161 atcaaaaagc atctaacatt aagttttgta gaattcacaa ttctagctat tatcacttct
    50221 caaaataaaa acatcgttct tcttaaagat ttaattgaaa caatccacca taaataccct
    50281 caaactgtta gagctctcaa taatttaaaa aagcaaggct atctaataaa agaacgctca
    50341 actgaagatg aaagaaaaat tttaattcat atggatgacg cgcaacaaga ccatgctgaa
    50401 caattattag ctcaagtgaa gcaattatta gcagataaag atcatttaca tcttgttttt
    50461 gaataatatc tctattacgc aagtgtgctg tattctaaag tgcacttgtg ttttctattt
    50521 tttaataaaa actcagcaca ttatgaacaa ctatctattt tctatatctc ttaaaaccat
    50581 ttccgaaatt aaacctcagc acattcaaag ccccacttta ttcttaaaaa tattttttaa
    50641 cttatatgta ttaaaccgct ttcattataa aaaatatctc tatattttat ctgtttttat
    50701 taattgaaat agcgtgattt tgcggtttta agccttttac ttcctgaata aatcttttag
    50761 caaaatattt attttataag ttgtaaaact tacatttaaa tttaattata aatatagatt
    50821 ttagtattgc aatacataat tcgttatatt atgatgactt tacaaataca tacagggggt
    50881 attaatttga aaaagaaaaa catttattca attcgtaaac taggtgtagg tattgcatct
    50941 gtaactttag gtacattact tatatctggt ggcgtaacac ctgctgcaaa tgctgcgcaa
    51001 cacgatgaag ctcaacaaaa tgctttttat caagtcttaa atatgcctaa cttaaatgct
    51061 gatcaacgca atggttttat ccaaagcctt aaagatgatc caagccaaag tgctaacgtt
    51121 ttaggtgaag ctaaaaaact taatgactct caagctccaa aagctgatgc gcaacaaaat
    51181 aacttcaaca aagatcaaca aagcgccttc tatgaaattt tgaacatgcc taacttaaac
    51241 gaagagcaac gcaatggttt cattcaaagt cttaaagacg atccaagcca aagtactaac
    51301 gttttaggtg aagctaaaaa attaaacgaa tctcaagcac cgaaagctga caacaatttc
    51361 aacaaagaac aacaaaatgc tttctatgaa atcttgaaca tgcctaactt gaacgaagaa
    51421 caacgcaatg gtttcatcca aagcttaaaa gatgacccaa gtcaaagtgc taacctattg
    51481 tcagaagcta aaaagttaaa tgaatctcaa gcaccgaaag cggataacaa attcaacaaa
    51541 gaacaacaaa atgctttcta tgaaatctta catttaccta acttaaacga agaacaacgc
    51601 aatggtttca tccaaagctt aaaagatgac ccaagccaaa gcgctaacct tttagcagaa
    51661 gctaaaaagc taaatgatgc acaagcacca aaagctgaca acaaattcaa caaagaacaa
    51721 caaaatgctt tctatgaaat tttacattta cctaacttaa ctgaagaaca acgtaacggc
    51781 ttcatccaaa gccttaaaga cgatccttca gtgagcaaag aaattttagc agaagctaaa
    51841 aagctaaacg atgctcaagc accaaaagag gaagacaata acaagcctgg taaagaagat
    51901 ggcaacaagc ctggcaaaga agacaacaaa aaacctggta aagaagacgg caacggagta
    51961 catgtcgtta aacctggtga tacagtaaat gacattgcaa aagcaaacgg cactactgct
    52021 gacaaaattg ctgcagataa caaattagct gataaaaaca tgatcaaacc tggtcaagaa
    52081 cttgttgttg ataagaagca accagcaaac catgcagatg ctaacaaagc tcaagcatta
    52141 ccagaaactg gtgaagaaaa tccattcatc ggtacaactg tatttggtgg attatcatta
    52201 gccttaggtg cagcgttatt agctggacgt cgtcgcgaac tataaaaaca aacaatacac
    52261 aacgatagat atcattttat ccaaaccaat tttaacttat atacgttgat taacacattc
    52321 ttatttgaaa tgataagaat catctaaatg cacgagtaac atcttttgtt gctcagtgca
    52381 ttttttattt tacttacttt tctaaacaac ttctgaaacg cctcaacact ttctactctg
    52441 attacatata cgacattttt agacattaaa aaatcgaact agacaagatg ctcattgcat
    52501 ttcgtactag ttcgattcat gaataattag atttaaaatg tcatttgaat ccaagtgaca
    52561 acattattta tatttagaat attaacgtta gtataaacgt ccaaacacaa ataaaggcaa
    52621 caaatataat actgtatttt aacgtcattt ttaataatgc agattcttca ccaacttttt
    52681 taacagctgc agtcgcaatg gcaattgatt gtggtgaaat aagtttcgct gctacaccac
    52741 ctgcagtgtt agctgccaca agtaatgaac cgcttgttga aatttgttgt gctactgtcg
    52801 cttgaatagg tgcgaataaa gtattgttat ttacaactga accagtcata aacacaccaa
    52861 tccaacctaa tactggagag aataatggga aaattgctcc cgctttagca ataccttgtc
    52921 caattgctac agtcaaacca ccgtatgtca taactttagc aatagctagg atagctgaaa
    52981 ttgtaaggat cggtaaccat aattctttaa ttgcttcgac caataaagca cctgcacttt
    53041 tccattttaa cttcgtaatt aaaattgtaa taattactgt taataaaatc gctgtcccag
    53101 ttgcaccaat taaatcgaga cgcaacgcaa ttcctttagg cgataaatca ctcacagtat
    53161 ttggaattgg caattttatt actaaacttt caagtgcacc tccaggttgg aataattttt
    53221 tgaagaatgg tgcactccat actaatacaa aggcagttaa aatgacgaac ggactccaag
    53281 caaagacaat ttctttaggc gttcgttttt gaattttatg ttcagacgct tccaatctga
    53341 aaatgttttt cggtttaaat ttacgacaaa caaatgctaa caccaccatt gttgctagtg
    53401 atggaataat gtctgctagt tctggaccat ggaatattgt taataataat tgtaatccag
    53461 tatatgtacc actcactgtt aaaatgacag gtaaaatttc tttaatacct ttcataccat
    53521 ctacaatgaa tactaaaaca aatggaataa taaagtttaa aattggaagt gttaatgctg
    53581 agtatctcgc aacatctaat gttgtaacgc ctccacttaa gttaaaagta tcaataatac
    53641 taactggtaa accaattgca ccaaaggcac ccgccgcacc attagcaatt aaacataaca
    53701 tcgctgcttt taatggttca aatccaagtt gaattaataa tactgcacaa atcgcaattg
    53761 gcacaccaaa tcctgctgca ccttctaaaa atgcgttgaa acaaaatcca attaataata
    53821 gttggattct ttggtcctct gaaatacttg caatactatc ttgaataata gaaaattgtc
    53881 ctgttttaat agaaacttta tataaccaaa ctgccattaa cacgatatat cctattggga
    53941 aaataccggc aacaacgcct tctgtaatcg cacctgctga tacacgcgct ggtaattcaa
    54001 atacaaataa agccacaatc aatgtaacaa ccaaagttgt caatgctgca taaatgcctt
    54061 tcattttaaa aacggttaag cataataaaa ataaaataat aggtactgct gcaactaagg
    54121 ctgataatcc aacattatcg aatggattta cagtaagtag tgtcataatg actccctctc
    54181 tttatataaa atatttatca tcctgattaa tctacaacct atttcaactt atattttgcg
    54241 atgatcacat atttaaaatg taacactcct atatgtgaca ggcaatcgaa tttttacaaa
//
LOCUS       DAKSQE010000003        54030 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.003, whole genome shotgun sequence.
ACCESSION   DAKSQE010000003 DAKSQE010000000
VERSION     DAKSQE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 54030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54030
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.003"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(546..653)
                     /locus_tag="O0V33_000111"
     CDS             complement(546..653)
                     /locus_tag="O0V33_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCX8974811.1"
                     /translation="MFNLLIDIMTSALSGCLVAFFAHWLRTRNNKKGDK"
     gene            complement(787..1173)
                     /gene="gtxA"
                     /locus_tag="O0V33_000112"
     CDS             complement(787..1173)
                     /gene="gtxA"
                     /locus_tag="O0V33_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HCX8974812.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLHIEYMISHI
                     TGFIVAFVISYYLNCYFVYRVKPTWRKFISFPITQIVNISLQTVLLYVFVSWLNLPAE
                     IAPFAGLIITIPITFILSKWILKDSN"
     gene            1441..2583
                     /locus_tag="O0V33_000113"
     CDS             1441..2583
                     /locus_tag="O0V33_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCX8974813.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGKTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDV
                     NGDLLQMNGDNLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            2643..3302
                     /locus_tag="O0V33_000114"
     CDS             2643..3302
                     /locus_tag="O0V33_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8974814.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDDNGGA
                     N"
     gene            3484..4695
                     /locus_tag="O0V33_000115"
     CDS             3484..4695
                     /locus_tag="O0V33_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCX8974815.1"
                     /translation="MQSSKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILASGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTPKEELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
     gene            complement(4818..5291)
                     /locus_tag="O0V33_000116"
     CDS             complement(4818..5291)
                     /locus_tag="O0V33_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCX8974816.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCIPELDGKMSYTIAVTGDNSADIEKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQADTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            5404..6060
                     /locus_tag="O0V33_000117"
     CDS             5404..6060
                     /locus_tag="O0V33_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCX8974817.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPDAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQKQNKHT
                     "
     gene            complement(6123..6929)
                     /locus_tag="O0V33_000118"
     CDS             complement(6123..6929)
                     /locus_tag="O0V33_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCX8974818.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKNLDILLIAGDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPF
                     IVNEEWAIIGHTGWYDYSFAAQRFSLDELQRGKHYGATWQDKERISWGTSDQNLSKIA
                     AEQVKKDLLEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDI
                     PYSIMGHVHFRKSVMDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
     gene            7215..8624
                     /locus_tag="O0V33_000119"
     CDS             7215..8624
                     /locus_tag="O0V33_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCX8974819.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
     gene            8762..10840
                     /locus_tag="O0V33_000120"
     CDS             8762..10840
                     /locus_tag="O0V33_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCX8974820.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAIEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITQG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQ
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPDNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKD"
     gene            complement(10980..12808)
                     /locus_tag="O0V33_000121"
                     /pseudo
     CDS             complement(10980..12808)
                     /locus_tag="O0V33_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
     gene            13040..14539
                     /locus_tag="O0V33_000122"
     CDS             13040..14539
                     /locus_tag="O0V33_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCX8974821.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQID
                     TIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDDVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDS
                     VTNKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSW
                     LKPLHMTHTSFKQNNNKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASMLNQYKDQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGKIILRKSKLTTFLSWLTLCLCIAIALILYALPY
                     LILGSNNWSFVLTWLPIEIKLTLTTAFIALFSMLITLLLILHTTTIKKP"
     gene            complement(14646..15497)
                     /locus_tag="O0V33_000123"
     CDS             complement(14646..15497)
                     /locus_tag="O0V33_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCX8974822.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAV
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYKTWK
                     DGMNPIK"
     gene            complement(15819..16754)
                     /locus_tag="O0V33_000124"
     CDS             complement(15819..16754)
                     /locus_tag="O0V33_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCX8974823.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEAMKPHIDNETIVVC
                     TMNGLKHEEIIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            complement(16980..18380)
                     /locus_tag="O0V33_000125"
     CDS             complement(16980..18380)
                     /locus_tag="O0V33_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCX8974824.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLVA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLIAVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEMWYIVSSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIV
                     AFLVILLLVPKNQTNL"
     gene            complement(18674..19369)
                     /locus_tag="O0V33_000126"
     CDS             complement(18674..19369)
                     /locus_tag="O0V33_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8974825.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            complement(19369..20310)
                     /locus_tag="O0V33_000127"
     CDS             complement(19369..20310)
                     /locus_tag="O0V33_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCX8974826.1"
                     /translation="MKKIKYILVVLVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGV
                     LKEAPIKDPKKAMIATQQGFKKKFDQTFFNSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            complement(20327..20962)
                     /locus_tag="O0V33_000128"
     CDS             complement(20327..20962)
                     /locus_tag="O0V33_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8974827.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIILTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLILGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            complement(20959..22185)
                     /locus_tag="O0V33_000129"
     CDS             complement(20959..22185)
                     /locus_tag="O0V33_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8974828.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSVSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRNIGYVIQQIGLMTHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAELSGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQAEATLNDAVHIMRQ
                     KRVDTIFVVDRDNHLLGFLDIEDINQGIRGHKSLRETMQQHIYTVQIDSKLQDSVRTI
                     LKRNVRNVPVVDDQQRLVGLITRANVVDIVYDTIWGDSEDTVQAEYVGEDTASSKVHE
                     QHTTNVKVRDIGDDKS"
     gene            complement(22548..22631)
                     /locus_tag="O0V33_000130"
     CDS             complement(22548..22631)
                     /locus_tag="O0V33_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8974829.1"
                     /translation="MGVCPPVTMDACALLQKFDFCNNISHF"
     gene            complement(22711..22820)
                     /locus_tag="O0V33_000131"
                     /pseudo
     CDS             complement(22711..22820)
                     /locus_tag="O0V33_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22855..23454)
                     /locus_tag="O0V33_000132"
     CDS             complement(22855..23454)
                     /locus_tag="O0V33_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HCX8974830.1"
                     /translation="MSEKQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLEE
                     IQARREEIKYYIAEAIKAEKAGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            23635..25272
                     /locus_tag="O0V33_000133"
     CDS             23635..25272
                     /locus_tag="O0V33_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCX8974831.1"
                     /translation="MIKDKQLHNEKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPA
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMKHLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIIVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSY"
     gene            25579..26931
                     /locus_tag="O0V33_000134"
     CDS             25579..26931
                     /locus_tag="O0V33_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HCX8974832.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSATFTEHEDCLYLNIWKQHNDQSKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKGFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSIWTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLESARNKAQHFQKLMLDYLDTDDVTSLSTDDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
     gene            complement(26993..28180)
                     /locus_tag="O0V33_000135"
     CDS             complement(26993..28180)
                     /locus_tag="O0V33_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCX8974833.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHEAPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGSLGLIVVFTLKNNHYAKTF
                     KSS"
     gene            complement(28574..29350)
                     /gene="fetB"
                     /locus_tag="O0V33_000136"
     CDS             complement(28574..29350)
                     /gene="fetB"
                     /locus_tag="O0V33_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCX8974834.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAVVQLII
                     LGFLLHYIFKINDKWLLVLCVFVIIVNASWNTISRSSPVMHHVFLISFVAIFVGTALP
                     LVGTIATGAIQFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TTKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            complement(29343..30005)
                     /locus_tag="O0V33_000137"
     CDS             complement(29343..30005)
                     /locus_tag="O0V33_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCX8974835.1"
                     /translation="MLLEIKDLVYEASDRVILNHINLKVDKGETIAIIGPSGSGKSTF
                     QKLICNLISPTSGTLYFKGKPYDDYEPEILRRHISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDKKRAKQLIKDVGLGHYQLSSKIEHMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDINNKEKIENIIFKLADQGVAIMWITHSDDQSMRHFQKRITIVDGQISNTEELNQ
                     HE"
     gene            complement(30351..31427)
                     /locus_tag="O0V33_000138"
     CDS             complement(30351..31427)
                     /locus_tag="O0V33_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCX8974836.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVDGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLTNDKYLTAKAFDNRYGCALAVEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKKHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSVRLVTEIVRSLNDETYKNIMW"
     gene            31665..32120
                     /locus_tag="O0V33_000139"
     CDS             31665..32120
                     /locus_tag="O0V33_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCX8974837.1"
                     /translation="MKKLCSLIVVALVCVIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            complement(32288..33865)
                     /locus_tag="O0V33_000140"
     CDS             complement(32288..33865)
                     /locus_tag="O0V33_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HCX8974838.1"
                     /translation="MTFLTVMQFIVNIIIIGFLLTVMVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLTNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLSASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            34061..34810
                     /locus_tag="O0V33_000141"
     CDS             34061..34810
                     /locus_tag="O0V33_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCX8974839.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAVFTQQAPWGFSFPISMNISYLLAVIISLAIFRRMHEDAFVPMIRMQIVASA
                     KYKWHILISFTLSIVLFLLLNHFAIFALNVDSFTVFDIYPLIREYGSISLIAIFSLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSVILETLYYYPYINTWWEFIPALILAS
                     SATYLYLKSNRNFMVSYFYQLAVILVLHIFM"
     gene            complement(35034..36227)
                     /locus_tag="O0V33_000142"
     CDS             complement(35034..36227)
                     /locus_tag="O0V33_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCX8974840.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVVARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSLLKKPTPR
                     PANSL"
     gene            complement(36239..36988)
                     /locus_tag="O0V33_000143"
     CDS             complement(36239..36988)
                     /locus_tag="O0V33_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8974841.1"
                     /translation="MIKIKDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDQPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKDVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            complement(36981..37796)
                     /locus_tag="O0V33_000144"
     CDS             complement(36981..37796)
                     /locus_tag="O0V33_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8974842.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIVGLNPERLRVTGDITFDGKSMLSLSETQLKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDPKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEHGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            complement(37793..38662)
                     /locus_tag="O0V33_000145"
     CDS             complement(37793..38662)
                     /locus_tag="O0V33_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8974843.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            complement(38659..39594)
                     /locus_tag="O0V33_000146"
     CDS             complement(38659..39594)
                     /locus_tag="O0V33_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8974844.1"
                     /translation="MFKFILKRIALMFPLMIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTMISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            complement(39607..41205)
                     /gene="cntA"
                     /locus_tag="O0V33_000147"
     CDS             complement(39607..41205)
                     /gene="cntA"
                     /locus_tag="O0V33_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HCX8974845.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            complement(41348..42649)
                     /gene="cntM"
                     /locus_tag="O0V33_000148"
     CDS             complement(41348..42649)
                     /gene="cntM"
                     /locus_tag="O0V33_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HCX8974846.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKEKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFSQDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMAILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGEMLGIKTPMINQFLTRYEA
                     SCKTYKDMHQDQQLSSQFNTNLFEGDKALVTKFLEINRTLS"
     gene            complement(42642..43460)
                     /gene="cntL"
                     /locus_tag="O0V33_000149"
     CDS             complement(42642..43460)
                     /gene="cntL"
                     /locus_tag="O0V33_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HCX8974847.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEVLETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSIASNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            complement(43471..44292)
                     /gene="cntK"
                     /locus_tag="O0V33_000150"
     CDS             complement(43471..44292)
                     /gene="cntK"
                     /locus_tag="O0V33_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HCX8974848.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKAIEIIYETYVHYVIPVSQVTTE
                     IQHLVETYVREQQWNHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            complement(44867..45634)
                     /locus_tag="O0V33_000151"
     CDS             complement(44867..45634)
                     /locus_tag="O0V33_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCX8974849.1"
                     /translation="MNIRHIRNATAVIEYGGKRVLIDPMLSDKGAFEPFPNSPRQDEN
                     NPLVELPLAIEDIINNIDLVILTHLHIDHFDPKAIEVLPKDIKIYTQNEADASEVEGY
                     DFTNVSVFNDVTHIGEIELIKTDAQHGHGEILKMTGHVHGLVLKHSEEPTLYLAADTV
                     WFDGVEKTLKAHHPDIIVLNGGANQFFEGGPLVMDEHDVLKVAHTLSLAQIVVVNMEA
                     VNHWHLSREELNEFINSNDLGNRVVVPNDGELLTFEK"
     gene            complement(45735..46553)
                     /locus_tag="O0V33_000152"
     CDS             complement(45735..46553)
                     /locus_tag="O0V33_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCX8974850.1"
                     /translation="MKRLENKVAVVTGASTGIGQASAIALAQEGAYVLAVDIAEAVSE
                     TVDKIKSNGDKAKAYIVDIASEQQIDNFASEIREQIGHVDVLFNNAGVDNAAGRIHEY
                     PTDVYDKIMNVDMRGTFLMTKMMLPLMMTKGGSIVNTSSFSGQAADLYRSGYNAAKGA
                     VINFTKSIAIEYGRDGIRANAIAPGTIETPLVDKLTGTSEDKEGKAFRENQKWMTPLG
                     RLGKPEEVGKLVVFLASDESSFITGETIRIDGGVMAYTWPGEMLSDDSWKRTLE"
     gene            46788..48326
                     /locus_tag="O0V33_000153"
     CDS             46788..48326
                     /locus_tag="O0V33_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCX8974851.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMT
                     WVISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAMMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     VSVVVLLPTILLVTTKLIIPRLGQYDDRLMHDDHEETSSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIAWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            complement(48438..48860)
                     /locus_tag="O0V33_000154"
     CDS             complement(48438..48860)
                     /locus_tag="O0V33_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCX8974852.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDKL"
     gene            complement(49313..49726)
                     /locus_tag="O0V33_000155"
     CDS             complement(49313..49726)
                     /locus_tag="O0V33_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCX8974853.1"
                     /translation="MSKKHVFIIIGVILCICIVATVIHFKMKYDEKEKQKAIYYKEQQ
                     ARITLYLKHNTKEPNTIKSVHFTKLETSPMGSAVIEGYINENKKDDFVAYASPENNFQ
                     FVGDIILSKNLSEIIKIKTKSPDEIKEELDKKEGR"
     gene            complement(49744..50163)
                     /locus_tag="O0V33_000156"
     CDS             complement(49744..50163)
                     /locus_tag="O0V33_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCX8974854.1"
                     /translation="MSKKKILILTNIMLIILISVAGIYFKMKYDEKEKQKSIYYKEQQ
                     ERITLYLKHNTIEPNTIKSVHFTNLETSPMGSAMIEGYINENKKDNFTAYATPEHNYQ
                     FGGAMIESERLSELLKPAQKLKSPDDIKKELNKKKGH"
     gene            complement(50415..50841)
                     /locus_tag="O0V33_000157"
                     /pseudo
     CDS             complement(50415..50841)
                     /locus_tag="O0V33_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162200.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
     gene            complement(50868..51278)
                     /locus_tag="O0V33_000158"
     CDS             complement(50868..51278)
                     /locus_tag="O0V33_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCX8974855.1"
                     /translation="MLKKKILMLTSIILIILISVASIYFKMKYDEKEKQKSIYYKEQQ
                     ARITLYLKHNTIEPNTIKSVHFTNLETSPMGSAVIEGYINENKEDDFVAYASPENNFQ
                     FVGDLIKSERLSELLKPAHQLKSPDDIKKELNKK"
     gene            complement(51556..51978)
                     /locus_tag="O0V33_000159"
     CDS             complement(51556..51978)
                     /locus_tag="O0V33_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCX8974856.1"
                     /translation="MTKKHVFIIIGVILCICIVATVIYLKVKYDEKEKQKSIYYKEQQ
                     ERITLYLKHNTKEPNTIKSVHFTSLKTGPMGDAVIEGYINENKKADFVAYGSPEHHYQ
                     FGGSLIKSKNLSTLLKPAHQTKSPDEIKKELESKKNDR"
     gene            complement(52230..52925)
                     /locus_tag="O0V33_000160"
     CDS             complement(52230..52925)
                     /locus_tag="O0V33_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCX8974857.1"
                     /translation="MTVLTDKIAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKDKLQ
                     NVANQLAQDSVKVVPTDVTKKEEVDELIKMAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTAKAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITATYNPTDRKKLEPQDIAEAVLYALTQP
                     KHVNVNEITVRPV"
     gene            53224..53505
                     /locus_tag="O0V33_000161"
     CDS             53224..53505
                     /locus_tag="O0V33_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCX8974858.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEIKKLDDVRMPFSSAHDAQVKLYNVVYSYNGIKRNFKQVENEGF"
     gene            53441..53624
                     /locus_tag="O0V33_000162"
                     /pseudo
     CDS             53441..53624
                     /locus_tag="O0V33_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501240.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(53763..53954)
                     /locus_tag="O0V33_000163"
     CDS             complement(53763..53954)
                     /locus_tag="O0V33_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8974859.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
BASE COUNT        17397 a        10204 c         8048 g        18381 t
ORIGIN      
        1 taatacttat tacaagtaca tcatatatta gttgataacg attatcaatg tcgcgtggat
       61 ttgtgacaca tttcttttaa aaattcacaa ggttatgggg cagaaatgat aaagagccac
      121 taatgattta ttatgtaatg attctttatc cttagccaca gctaatatgt acttaaaaat
      181 aggaatacat gagtaaaact ctgtgttggg gccccgccaa cttgcattgt ctgtagaaac
      241 tgtggatcca atttctctat gttgggtccc tgagtatagc cttgtagagt ctagtacatt
      301 gatttgtatc ccaatgtccc tataattgat tattcgcttt atctaatgat cctatgactc
      361 aactattaaa tcatttttcg aaatacttaa ttctaatgta attaaatcca tttattgtaa
      421 tattgcaaaa atacattgca caccttgttc atcaatgcta taattagtta cataataaat
      481 tgaacatcta aatacaccaa atcccctcac tactgccata gtgaggggat ttatttaggt
      541 gttggttatt tgtcaccttt tttattgttg cgcgttcgta accaatgtgc aaaaaacgca
      601 acaagacagc cgcttaaagc tgaagtcatg atgtcaatta ataaattgaa catccgtcat
      661 acacctcctc tctgcgttaa agtaacgccc gagatgttag gcgaccatta tattatatca
      721 tttatttatt atattccacg caatattaag acccttccaa agtatttttt agtggtttac
      781 gctactttaa ttactatctt ttaaaatcca tttagataat ataaatgtga tgggtatcgt
      841 aataattaaa ccagcaaatg gtgcaatttc tgctggcaaa tttagccagg atacaaatac
      901 atataataaa actgtttgta agcttatgtt gacaatctgc gtaattggaa aactaatgaa
      961 ttttctccaa gtaggtttta ccctgtaaac aaaataacaa ttcaaataat acgaaatcac
     1021 aaaagcgact ataaatccgg taatatgact aatcatatat tcaatgtgta ataattttaa
     1081 cagcaataaa tagacaacat aatagtttaa cgtattaatg ccgccaacaa tgataaattt
     1141 taaaatttca gcatgcgttt gtgttagttt catatgtgta actcctcaac atcaaaatat
     1201 atgtataact gcgttctcca acatactcga atatgcgagc caatccgctt ctcttcaaat
     1261 atacttattt cagtttttat accctttcac agcaaattta gtctctttcc cctcatcctt
     1321 atacgccatt ataatgtaac tgatttatcg cgcgactcat tagcactata gagattactt
     1381 tagttcacta gtaattttat atacaataag agcgacaaca gtaatgagag gatgtctact
     1441 atgcaattac aaaaaattgt catcgctcct gactcattta aggaaagtat gaccgcacag
     1501 caagttggca atattataaa acaggcattt actaatgttt atgggaaaac ccttcattat
     1561 gatatcattc cgatggctga tggtggtgaa ggcaccacag atgctttaat gcatgcaaca
     1621 ggtgctacta agtatacagt catcgttaat gaccctttaa tgcgacctat tgaagcatgt
     1681 tatgcacgcg cagacgaaca acaaattgca attattgaaa tggcagcagc gtcaggtttg
     1741 gatttattag aaaaagagga acgtaatcct ttatacacat catcatatgg taccggtgaa
     1801 ctaattaaag atgcattaaa tcatggtgct aagaccatta ttttagggat tggtggcagt
     1861 gcaacaaatg atggtggtac aggtatgcta agtgcactag gcgtaaagtt tactgatgta
     1921 aacggggact tattacaaat gaatggtgat aatcttgctc acattgcaca aatcgatata
     1981 accaatctag attcgcgatt aaaagaagtg acctttaaag tggcctgcga tgtttcaaat
     2041 cctttattgg gtgaaaatgg tgctacctat atttatggtc ctcaaaaagg cgctgatgca
     2101 aagatgatac caaagttgga tttcgcaatg tcgcattatc atgataagat aaaaatgtgc
     2161 acaggaaagt cagttaatca aataccaggt tctggtgcag ctggcggtat gggcgcagca
     2221 ttattagcgt tttgtgagac aactttaaca aaaggtattg atgtcgtctt tgacattaca
     2281 gattttcatc aaagaattaa agatgcagac ctcgttatta ctggagaagg acgcatggat
     2341 tatcagacca tctttggtaa aacacccgta ggcgttgcgt tagctgcaaa acaatatcat
     2401 attcctgtca tcgcgatttg tggcagtcta ggcgaaaatt atcaacatgt ttacgatttc
     2461 ggtattgata gtgcctattc tataatctct tcacctagca ctttagaaga tgtcctacaa
     2521 aatagcgaac aaaatttatt aaacactgca actgacattg ctcgtattct gaaattacaa
     2581 taatgtcaaa gtaaatcatc agctttatta tttgcagtta aaacttgaat gaggtgaaac
     2641 ccatgaaaag aactgataaa taccgtgatt catatcaata cgacaatcaa aatcaaaatc
     2701 atcgtcgtca atctgaagac gcatcgtata gacaacaata tgctaaaggt gatcctgaag
     2761 aacacccaga acgatactat aacggtagag attatcgaag agaacaaatt cttgaagaag
     2821 aaaacgagaa atcccgccgt tcaaaaaaat ggttatatat cattattgcc attctcttaa
     2881 ttattgtcgc tatttttgtc acacgcgcct tacttaacaa tgatagcgat aaagttagta
     2941 atgaccctaa agtctctcaa aattataaaa aacaagttga aaatcaagac ggccaaatta
     3001 accagcaagt cgataatgct aaagaaaata ttaaaaacaa ccaaaaaact gatgacatta
     3061 ttaaaaattt acaaaatcaa atcgataact tgaagcagca agaacaaaac aaagctgatt
     3121 ctaagctaac tcaattttat caagaccaaa tcaacaaatt gacagaggca aataatgcac
     3181 ttaaaaacaa tgcaagccaa ggtaaaattg aaagcatgtt aaatgatatt aatacaaaat
     3241 tcgacagtat taaatctaaa ttagaaagct tatttaaaga tgacaatggt ggcgctaatt
     3301 aattattaaa cctgcttttg atgataaaca ttaattccct atactttatc tgtatcacta
     3361 cgttattcgt gatgatgcat taagagtata gggatttttt atataaactt gtattctaac
     3421 tacatacaaa tacacacaaa acgtatataa tttatataat tatataaatt aaggagtctg
     3481 tttatgcaat cttcgaaaac caaaacgaag catttttcat ttttattgct aatcacgtta
     3541 ggtgtcatga ccgcttttgg ccctctaact atagatatgt acgtaccatc attacctaaa
     3601 gtgcaaggtg attttggttc tactacatca gaaattcaat taacattatc attcacaatg
     3661 attggtcttg cactaggcca atttatcttt ggacctttat ccgatgcttt tggtcgcaaa
     3721 cgtattgctg tatccatttt aatcattttc attttggcat caggtttgtc tatgtttgtt
     3781 gatcaattgc cattattctt aactttacga tttattcaag gtttgacagg tggtggcgtc
     3841 atcgttattg ccaaagcctc tgctggtgat aaatttagtg gcaacgcact cgctaaattt
     3901 ttagcatctt taatggtagt taatggcatc atcactattc ttgcaccatt agccggtgga
     3961 ttagctttat ccgtagcaac atggcgttct atttttacaa ttttaacaat tgtggcactc
     4021 atcattttaa ttggcgtcgc ttctcaatta cctaaaacac ctaaagaaga attaaagcag
     4081 gtgaatttta gtagcgtcat taaagacttt ggaagtcttt tgaaaaaacc agcatttatt
     4141 attccaatgc tattacaagg tttaacttat gtaatgctat ttagttattc atctgcatcg
     4201 ccatttatta ctcaaaaatt gtataatatg acaccccaac aatttagtat catgtttgct
     4261 gttaacggtg taggtttaat cattgtcagt caagtcgttg ctttattagt agaaaaatta
     4321 catcgtcaca tattattaat cattttaact attatacaag tggtaggtgt tgctttaatt
     4381 atcctgacac ttacattcca tttaccactt tgggtcttac tcatcgcatt cttcttaaat
     4441 gtgtgtcctg tgacgtcaat tggaccgctt ggtttcacaa tggctatgga agaacgaaca
     4501 ggtggcagtg gtaacgcatc aagtttactt ggcttattcc aatttatctt aggtggcgct
     4561 gttgcaccat tagttggctt aaaaggcgaa tttaatacat caccatatat gattattatc
     4621 ttcattacag ccattctatt agtcagtcta caaatcattt actttaaaat gattaaaaag
     4681 caacatgtcg cataacactt caacataatt agaaccctag caaagatatc tatctttgtc
     4741 agggttcttc tttatgaatt atgagattga atcttcaact aaaattacgc cttcatatca
     4801 agtacatttc tattcaatca ccctttaaca ggcatccaaa tttctgtaat atatttttca
     4861 cttgtagtat caccatcctg atacaactca aaaaatggtg ctgattttac cgtatctgct
     4921 tgatattgat gtatgtagtg atgaacctct tccatttttt gttgaactgc tttaggtact
     4981 gctccctgtg cttcaaatac catatacttt gaacttgcta atgttatgac atcatatttt
     5041 tcaatatcag cactattatc tccggtaact gcaatcgtat atgacatctt accatctaat
     5101 tcaggtatac ataagccaag tatcccggct aaatcaccat tattttttaa ctgtaaatct
     5161 gcaattactc cctcttgata acatctttgc caaaaccctg ctatatgttg ctgagcctgt
     5221 cgtccatttt catacgtttc tttaatacct actaacgtta acgacgcaag ttgttgtaat
     5281 tgatattcca ttttatcact ccattaattt tattcactca tgtaaaacat atgtttagga
     5341 cattttgcat tgacgctata taattatatt aaatatttat tgaatcaatg cgaggactat
     5401 attatgaacc gaaaaatatt accagttaaa cctatcagtc agctatttcc tattcctatg
     5461 gtcatggggt gtgaaggtgt ttccgcagcc atgatgttgc aatacaataa ccaacatatc
     5521 cctgctacag aaattatgag acattggccg acacatccga acaatccaca taaaggttat
     5581 gttggacatc acttattcat taagcttggc aattatcatc aaacaatatt tccagatgca
     5641 tatgtaccat ttttacaaaa atacaatcct aatatcgtag acggcactgg taaatcctta
     5701 gaagatctta aaggtattat cgatcaaggt caacctgtgt taatatacca tactaatctt
     5761 ggttctaaac cattactacg tgttttccgc tttgataaca agccagcgaa acaagtttca
     5821 aatattcacg taacgttact cattgggtac gatgattact attactatta tatcgatcca
     5881 ctttggagtc atattcgtcg tggcctagtt ttacctgcca tcattccaaa tcgcaaacaa
     5941 attattaaaa ttcgtaaaga gaaaatggaa tacagcttta attcaccagg aagaaagtgc
     6001 atttatgtgc aaccacattc atatacaatt gaaaatcaga aacaaaataa acacacgtaa
     6061 gtaaacacat tactcgcgtg tgtaaaacat aaaatgcgca aactgggtca ggtttgcgca
     6121 ttttaaattt ctattatttg tatcgtctca tttatttcct ggtaaatatc ttctgaacgc
     6181 cattgtcttg gatagcctag acacggacag agataacatc tgccatcatc catcacactt
     6241 ttccgaaaat gaacatggcc cataatacta tatggtatat cgaacatcgc atacaaagga
     6301 ttgaaatcac ttgtcccaat aaatgcatta taaaaatcga atatacgatg cggcatagga
     6361 acaatgaaat cagggtgcgt cacaacatgt gtgactaaaa tcactcttct atttcctact
     6421 tctaatagat ctttcttcac ttgttcagcc gctattttag ataaattttg atctgatgtg
     6481 ccccaagata ttcgttcttt atcttgccaa gtcgcaccat aatgttttcc tctttgtaac
     6541 tcatctaatg aaaatcgttg tgctgcaaag ctataatcat accaacctgt atgtcctatg
     6601 attgcccatt cttcatttac tataaatggt tttcctacca agcactgtga catactctta
     6661 taattattcc aaatgtcttg cgtagtcata ctttcaactt cccataaatc atggtttcca
     6721 ggaacaaatt tcacaggtat atctaattgt tttgtcaatt gcgttataaa ttggtgcgtc
     6781 aattgataat gatttgagat atcccccgca atcaacaata tatccaaatt tttatactta
     6841 actatttcaa ccaatgcctc taagtaatct tcggatgttt ttttattatg acgatcaata
     6901 tgtaaatcgg cgattgtccc tatcttcata cccatacctt ctttactcaa attaaaatgt
     6961 tatttattat gccatagctt atctaatata tatagttaac tgcttcattt agatgattat
     7021 tttatatttt tgcataaaaa cttatatctt ttcaaaaaat cgataagttt tagttatcat
     7081 acccttaact atcaaatgtt ttttcttata tttaaaaaaa taattgcttt attaaatgga
     7141 tttctttagt atttataatt aagaaaaagc ttacacacaa cttttttatt tgctttatcc
     7201 tgaggaggaa aattatggca agaaaattgc atagagagtt gaataacaga cacatccaat
     7261 tgatagcaat tgggggcgca attggaactg ggttattcct aggatcaggt caaacaatat
     7321 ccttaactgg tccatcactg ttattcacat acatgattat tggggttgta ctattcgctt
     7381 ttatgcgcgc attaggcgaa ttgttgttga gcaatacaag atttaattca tttgttgata
     7441 ttgcaaatga atatttaggc ccttttggtg gctttgtcat tggctggact tactggctat
     7501 gttggattgt atcaagtatg tcagacctaa ctgcgatggg acaatacttt gcattttggt
     7561 atccacaagt cccaaattgg attaccgtgc tatttattgt tttaatcttg attagcttca
     7621 acttattagg tgccagatta tttggtgaac tggagttttg gttctctatt attaaagttg
     7681 ttacaattat tgcgatggtt atcgttggtc ttgtattaat ctttttctca tttaaaacac
     7741 attatggaca tgcatcattc acaaacttaa tcagtcacgg cggcatgttc cctggaggga
     7801 catttggttt cttaatgtca ttccaaattg ctgtatattc attcattggt attgaactta
     7861 taggtgtaac tgctggcgaa acgaaagatc ctgaaaagac tttaccgaaa gcaattaata
     7921 atgtacctat ccgtatttta ttattctata tcggtggtct attagtaatt atgtcagtca
     7981 taccttggaa tgatatcgat ccaaatagta gccctttcgt taaactcttt acattaatcg
     8041 gcgtgccatt tgcagcaggt gtcgttaact ttgtcgtgct aactgccgcg gcctctgcta
     8101 caaatagtgg aatctattcg aatagtcgta tcttattcgg actgtcacaa caagggttag
     8161 gtcctaaagt tttaaataaa acgaatagtc atggcgtgcc ttatttatca atgttagttt
     8221 catcaattgc attacttata gcagccttgt taaactacat ttttcctaat gcaattcaac
     8281 tattcatata cgttacaacg ttatcaactg tgttgttttt agttgtttgg gcaatgatta
     8341 ttgtcgctta tctaatgtat ttgaaaaagc atcctgaggc acataaaaac agtaaattta
     8401 agttgattgg tggtaagcct attgcttaca ttattttagc tttcttcttc tttgtattca
     8461 ttttattatt ctttagtgac gaaacaagag cagctattta tatttcgcca ttctggttta
     8521 tatttttatt tttcttttat aaaaaataca aaacgaatgc tgaaaagctc gcatatgaac
     8581 aacgacaaaa tgacagcggt catttcagat atgacaatca ataatttaaa catctcgctc
     8641 tagtatcagt tcaaccgata ctagagtttt tttgaaaata acttcatttt atcaatatat
     8701 ctatgtcatt atgccttaaa agtgataaaa tattaactat taaaattttg aggagtattg
     8761 tatggcacta ttagaagcat ttttaatttt tatttttgct gtgattatta gttcggttat
     8821 caataatcga tttccacaaa ttcctactgc ctttattcaa attgcattag gtgtcgttat
     8881 tttcattatt ccaatacaag ttgatttcca attcaattct gaagtattta tgtttgccgt
     8941 tatcgcgcca ctactttttg tggaaggtac ccacgtctct cgaacaaaat tattagaata
     9001 tcgcaaacct atactattaa tgtcaatggc actagtgttt gcaactgtag tcggtgttgg
     9061 ctattttata cattggattt ggcctgcctt accaatgcca gccgcttttg caatagcagc
     9121 aattttatgt cctactgatg cagtagcagt atctgctatt acacgcggaa aattattacc
     9181 taaaggttct atgacgattt tagaaggtga atctttactc aatgatgctg caggtatcat
     9241 ttctttcaaa atcgctgtta cagcattagt aactggtacc ttttcattat tccaagctat
     9301 tgaacaattt atcatttcaa caatactagg tgtacttatt ggtgcaatta ttggattcgt
     9361 tgtcgttaga atacgtatag acttaaccgc taataaaggt ttaaaagata acaatacctt
     9421 aacttttatt caattattaa ctccttttgt cgtatacttt ttagcagaag aagttcatgc
     9481 ttcaggtatc attgcagttg taatcgcagg tctaatccat ggtttagaac gcgaccgttt
     9541 aattagagca caaacggaac tacaaatgaa ttaccatcaa atttggaaca catttagtta
     9601 tgccttaaat ggttttgtat ttgttgtatt aggttttatg attcctactg tagtcataga
     9661 tattttccaa acagagcctg acaacttttc atttttaata gtgatcacta tattaatcgc
     9721 tattgcaatt tatgcatgtc gatttgtttg ggtttatttc tggtacaaag atttttattt
     9781 cccgaaaaat atacaatctt atctagacga ggaacatgat tcacatgaaa caccaccttc
     9841 tcgtgtgcgt tacgcattta ttatgacgat gtgtggtatt cacggtacca tttcactttc
     9901 aatggcactt acattgccgt ttattattac acaagggcaa gcttttgaat accgtaatga
     9961 tctattgttt attgcatctt tcatggtatt aattagttta attttagcgc aaattgtttt
    10021 acctttaatt acaccatctg ccgaagatac tacttttaaa ggtatgactt atcaatctgc
    10081 caaaattttc attgttcaaa aagtgatcca gcattttaaa aacgaaagta aaaaagacaa
    10141 aaacgataca aattatcgcc cagtattaaa ccaatactat ggagaattgt tatttttatt
    10201 aaattcagaa cctgataatc aaaatacaaa agaactcaaa cgtttagaag atattgcaaa
    10261 agtaatcgaa acatctacac ttgagcgttt aatcgataaa ggtaaggcaa cataccagga
    10321 tattaataat taccgcaata ttgtcgaatt aacagagaca caccgtactg catcatttgt
    10381 tgaaaaattt gcaaacttct tcaagatgtt atggcttaga cttaaagcga tgaagcacta
    10441 caaagcctta aacaaagaat ctaagaagca agaatttgaa aattcattca aagatgttca
    10501 aaaaattatg cgtattgtga atcacaatat tattttacgc ttaaaagaag aacaaaatag
    10561 tacaaatgta cttgaggtta gcttagtcat taatcattac tatgatatga gtcgctcatt
    10621 aaagtggcgt gcacaacgtc gaaaagaacg tcaagaaaac agcaatcaaa tcataccgca
    10681 agctatgttc cataaccaca aattggaagc attgtactta caacgtcatc ttttagatga
    10741 attaattcgc aaaaataaaa tcaacaatat cgttgcagct caaattcgag aaaatatcaa
    10801 ttacaacgaa attgtcttgt ctttacagtc caaagattaa gcaagacata acaacacccc
    10861 cgtacatatg agacaaagtc attatcatct catatgtacg gggtttttat agtcaacatc
    10921 aaaaaatcgg attgatgaaa agtaaataac ctttcatcaa tccgatttga ttatagaatc
    10981 tattttttaa gtttaaatgg aattgtacat acgttaacat tcttttgata aattaaatac
    11041 aatttcatac gcaaactagt ttgattgtgt aataaattat gccaacgttt cttagtaata
    11101 aattctggta tcactactgt aatcatatag ttttgatcgt tggctttacg attaatctta
    11161 tcgataaaac gtgaaattgg tcgtataata ctgcgatatt ccgagtgtaa aataactaat
    11221 cttacatctg ggaaatgacg tttccatttc tcttggaatg ctttttcatc ttcatctcca
    11281 aatgatacat gtactgcaat aacatcatta ttcgcaagca tttgtgcgta ataaatagac
    11341 ttatcaactg cagttgtaat gcttgttatc ggaacaattg ctaaattacg atctactact
    11401 tctacattat gaacatcaat atcagaacgt aattgttctg cgatatctcg ataatgtttg
    11461 ttaattttca agaagaaaat caccacgaac ggcaagaaaa taagtatcgg ccaaacttgg
    11521 ctaaatttag ttatgagtaa aatcataaaa acaataaatg tcacgatacc accaagtaag
    11581 ttcacagaca acttacttaa ccaattctta ggacgttcat gaatccattt aataaccata
    11641 ccgaattgtg ctagtgtaaa tggaatgaat acaccaaccg catacaatgg aattaaatct
    11701 tcagtcatac catcaaatac aataattaaa ataatggcta atacacctaa aataataatc
    11761 gagttagagt agcctaagcg atcacctcta acagtaaaca ttctaggcat atatttatct
    11821 ttagacattg aagccgctaa catcggaaaa gctgtaaatc cagtgttggc tgctaatacc
    11881 aaaatcatca cggtcgttgc ctgtacaaaa tagaaggcag cattatcatc aaatatttgc
    11941 atagctaatt gtgataaaac tgtcgtttcc gtttgtggca taattccata aacgtatgct
    12001 aaaccaacga taccaactaa taaaaacgct aaaattgaac ccatagcaat taacgttttt
    12061 acagcattat tagcacttgg ttctctaaaa ttggttaccg catttgaaat agcttcaaca
    12121 cctgttaatg atgaagcccc tgatgaaaat gcttttaata gcaagaataa tgttactcca
    12181 ggaaccgcag ttcctactga tgcatgcata tgtggttgaa tatcacctgt cgccacacgg
    12241 aaagtaccta taaatattaa tatcactaac cctataatga aaagatatac tggataggat
    12301 aatacggtgg cagattcagt taaaccacgt aaatttaata ttaaaataaa aagtacaagt
    12361 aaacatgcaa tcagtacttt atgcccatat aaacttggga atgcagcaac aaatgcatca
    12421 gcaccagatg atatactaac agcgacagtc agtatgtaat cgactaataa tgagcctcct
    12481 gcaagcaatc cccatttttc tcctaaattg gtcttggaca ccatatacgc gccgccacct
    12541 ttaggatatg cataaataat ttgcctataa gacataatta aagcagctaa taagatcaga
    12601 acagcacctg caatcggtaa agtataccaa gttgcaactg cacccactac tgataatgta
    12661 atcagtattt gttcgggacc gtaagccact gaagataatg catccgacga aagaatcgct
    12721 aaccctttaa acttcgatat cttttcgtct tttagttctc tgttttttta ggttgcccta
    12781 taataagtct tttaaattga ttgaacataa tctcctattc ctttttatac tttttgatgg
    12841 atatcactat acgcctatcc ttccaaaaag tatagagagt taattaatat tttttatgta
    12901 attatacacc tattaacata taaggtgtca aaaataatgg taaatatacc tctttaatta
    12961 atttattcaa tagaactggt ataataaaat aaatctcatt aggcacttaa gtaaatttaa
    13021 catataaaaa ggaatgttta tgactactaa aaaactgtat tttctatcca tttctattat
    13081 cattttagtc gccatttcaa ttgctatata tataacatta aatagcaata cgaagacacg
    13141 gttaaccaat gattcgcaac aacaaataga tacaattatc gaacatgatt tacaaaaggg
    13201 acatattcct ggagcatcaa ttttaatagt aaaaaatggc aaagtttttt taaataaagg
    13261 ttatggttat caagatgttg ataaaaaagt caaagcttct cccacaacaa agtatgaaat
    13321 tgcttctaat acgaaagctt tcacaggtct tgcaatttta aaattagctc aagaaggtcg
    13381 attaaactta aatgatgacg tatccaaaca tgtgcctcat tttaaaatga actataatgg
    13441 tcaaaatgaa actattacga ttaagcaact tttggctcaa acaagtggta tacctagtga
    13501 tattacaagc gaagactccg taacaaataa aaataatcgt ttaaatgatg taacccgtgc
    13561 aattatgggt gatgaattac atcataagcc cggagaagaa tttgaatact caaatatgaa
    13621 ctatgattta ttaggtttaa ttatccaaaa cgttacgaag caatcctata caaaatatat
    13681 tacaaattca tggctcaagc ctttgcatat gacacataca tcattcaaac aaaacaataa
    13741 caaatcaaaa catgatgcta ttggctacga attacaaggt tcgacacctg tcgtctctaa
    13801 acctgaattt aacctttggg atacaccatc agcatatatg atgacatcaa ctgaagattt
    13861 ggaacattgg ataaaattcc aacttaatcc acctgataaa tacaaatcat tagttcaaca
    13921 atcacataaa aatttatcat caacaattgg tgaacctaat gccaatgcat atgcttccgg
    13981 ctggtttact aataatgatg aacatttagt gtttcattca ggaacgctcg ataacttttc
    14041 atcatttatt ttactaaatc caaaacaaaa ttatggaatt gttgtgcttg caaatctaaa
    14101 ttcggaatat gtacccaaat tagttgagca tcttaacaca caaattgtaa atcacaagcg
    14161 atattcgaca gttgcgtcta tgctcaatca atataaagat caatttaata ttgttaccgt
    14221 tttgatgaca acacttattt tattagcatt tatattctca gcttatcgtg cttggcaaat
    14281 gcgtcacggg aaaatcatcc tgcgcaaatc aaaacttacc acatttttaa gttggctaac
    14341 tttatgttta tgtatcgcta tagcactcat attatatgca ttaccatatc tcattctcgg
    14401 tagcaataac tggtcttttg tactgacttg gctgccaata gaaattaaat taacgttaac
    14461 tacagcattt attgcattat tcagtatgtt aattacactt ctgttaatat tacatacaac
    14521 gacaattaag aaaccataaa aaagacttgt tcaatcgcac atcaatccga aacatacata
    14581 tttatgtatg aggattagcg atttgaacaa gtcttcatta ttaacatgct atatgcagaa
    14641 accgattatt taattggatt catgccatct ttccaagttt tataaatcag agtaccacct
    14701 tgcgctttaa acttctcatt gcttacgcca cgttcaaatg gttgcgcagg ttgaatatca
    14761 atatttggtt caacaccaag ttgttcttta taccattctg caaattcaga acctttaaca
    14821 ggtgcatcat ctgctacatt atagatacca ttttcaaaat gaatagcttg aatagatgtt
    14881 tcaactgcat catcaagatg cacaaatgat gttacgccat ctgaaagtgt cacttgacca
    14941 tccataaatt gattataaat catgccatct tttccgtacc aagtacctgg accatataac
    15001 cagccaaaac gtaaaacaac gtattcatcc atacgggccg tttcttcttc tagaccaacc
    15061 acaccatcaa ccgttacttt tctatctcca gttgagttaa aatcaagtga agtttcctca
    15121 tttgctaatc cttcgcccgg ttcatacata aaggcaatac tttgggcaat tactttctta
    15181 acatcatgct ttttcgccgc atcaattaag tttttagaac cttcaatacg tactttcgta
    15241 tttgctgcca tatcaacatt ttttaaatcc gtaatttggt tgataatgat ttctggttta
    15301 aaatctgcta acgcttgatc aatggtatca gcttttaata tatcaccaat atatgctttt
    15361 acattaacag cagctagctt ttgttgacca ttctcagatg tagtaaaacc agcaacctca
    15421 tgcccctctt cttttagtct ttgaactaat ttaatgccaa taaggcccgt tgcaccagtt
    15481 acaaaaattt tactcattat aaacaccttt tctctatttg tcttttaata taattacttg
    15541 cttgatgagt gtacaaaatt cacgtaagac ttccaaatga tttgcctcaa tttttttcaa
    15601 agtgtcgtcg taaaaactgt ctagcaaatg ctagtagtat gtcgtagacc tatgacaaat
    15661 ctgaattatg acgaagacca atcaagaaaa acagcaccaa gatcatttca atttgaaagt
    15721 actttactgc tgttctttat ttattacatt tcaattttaa taatgaccgt aatatcacgt
    15781 gattgtcatt tccttgcaat cacacctaaa aacactaatt aatcctgtgc atgtcgctga
    15841 ctttctttag tatgaattaa atcagtaata aagcgattga ctggtgcttc aatatgacgt
    15901 tgtttaccta atgttgcaac tgcgccatta atataatcaa tttcagtctt tctattatta
    15961 acaattaaat cttgatacat ggatggataa tgcgcaccaa ctttttcatt taaatcaact
    16021 aaatattcaa atacttcatc aacatttaaa tgaacattat caatcgtcgc tacatgcact
    16081 atttcttgcg ttaatttata aatcaaacac ttcgcataac tactttcatt cagtgatgcc
    16141 atattacact ccaacactgt gcttaatgca tttgccgtac cattaacaca aatctttttc
    16201 caaatcgatt ggtataaatc tttactaatg acaccattca attccgcttc gttaagtaaa
    16261 tcagcaactt ttataacatt ttctttacct tcatcaacta gttcacctat ttcaactggt
    16321 ccactaccaa gtaaatgact gtgtccaggg ctttcaagac ctgccgtcca agtcgtaaca
    16381 cctctgacaa tttgtgattg agcaacatac tgcgcaatga tttcttcatg cttcaaacca
    16441 ttcatcgtac atacgacaat cgtttcatta tcaatatgtg gcttcatagc ttccatcact
    16501 tcttttaatt gcatagactt tggaaataga aagacaacat cgtaaatgct ttcgtccggt
    16561 tgatcattaa aatgatacat tggaatgttt aactcgaatg cctctccatt aatcgttata
    16621 tttaatccat gttgcttaac cgcttcaaca tgagatgtat atccgtcaat aagtgtgaca
    16681 tcatatcctg cttgaaatag tttggcacca aagccactac ctaatgcacc cgatccagca
    16741 attgcaattt tcatcctata aacccctctt catcgttgtt gttcattttt aaaatactac
    16801 tttcgcatat tagcgtcaat ttgaaaatca ttcgcttaat atacacacag cttttatcgt
    16861 ctataaaaga acacacctaa cattaaacgt caatacgcaa cattaagtgt gtatgataaa
    16921 cttactttca caaaatttcc tttaaaatcc ctacataata acttgctttc atttcagttt
    16981 tacaaattcg tttgattttt aggaactaat agtaaaatga ctaaaaatgc aacaattgct
    17041 agcaaggcat taaacatcat acctgtgaaa ccacctaagt tcaaatttaa attagctgct
    17101 aacacagtat aaaccgtacc agatactgct actccaaatg catttcctaa tgatgacgcc
    17161 attttatata cgcctgaagc aacacccgac ttatcatctg gcgcactagc aactgctgta
    17221 tcagttgatg gtgtagcata taatcctaaa ccagtaccaa acaataaata tccaactata
    17281 ctagatacga tataccacat ttctggtaaa aatgttaacg acaataagat taacccaatc
    17341 actgtaaagc cacttcctag taatagtggg cgtttcggac catgttgaga taaaatcttt
    17401 tcacctacac gaatcataga caacactgct attaaatacg ttaatgaaat ataacccgtt
    17461 tgcgaagaat taaatcctaa ttgttgttga taatacgtgt taatgacgat aagtgcacca
    17521 cctgctacac catttaataa gaagtttgaa atagttgcac cactgtatcc tctattttta
    17581 aaaattgaaa aatcaacgag tgggtgcttt attttatttt cgtaatatac aaaaccaact
    17641 aatgaacaga taaacactac aattaaacct agaattaacg gtgaaactaa accaaaatgg
    17701 gacgtctgtg taatgattac atttaaactt aacattgtca ctactaaaat gactaacccg
    17761 ataacgtcaa actttttagc ttctgctttc atacctttga ttggttctgc tttagtctca
    17821 ggtgcatgtt tgattaagta cattgctaat aatgttaata gaattgaaac aacaaatatt
    17881 gaacgccaac ctatatatgt agccactaag ccaccaaaca acgtacaaat accactacca
    17941 ccccaagaac caatagacca atagcttaag gcacgttgtc ttcttgtacc aatataatat
    18001 tcgttaataa tagcaagtgt ggatggcata atacatgctg cagacaaacc ttgaattatt
    18061 ctacctataa ttaaaaatgc tggcaaaggt gtaataatga tgagtaatga acctacaaca
    18121 tttaatatca atcctacata agtaattttg acgcgaccaa atttatcagc aacatcacca
    18181 gcacctacga taaacaaacc agcaaataag gcagataagc taacagcgat atttatcgtt
    18241 ccaacgtcac tactatatgt tgattgtaat gggacaacaa gattaacaag tgactgcgcg
    18301 aatagccaga aggtaataac ccctaaaacg atacctaaaa gcaatcggtt gtcacctcta
    18361 aattgttttg atgtgtccat gaattagata agcctcctat gtttaagaaa aacaaatttt
    18421 taatatgata tattaagaaa caatatcata aatttgcaga aaattataaa taaaacatct
    18481 cattttttag tatttttaca ataatcaacg ctttttatcc aaaaactttc atgataataa
    18541 tttctatttt agtgtaaacg catacaaaca caacctataa aaaggctact caaagattat
    18601 ttgatctcct tgagtagcct cattttttat aaatcatgaa acggaatcgc taaattataa
    18661 acatcttcta ctattacata ttaatacttt gcggccgatg attagattga tttttacgat
    18721 gtcgtgtcgt tgggtctaat cgtttttcta aaaatcttaa tagtacatca atgacgattg
    18781 caatgatagc aatcggaatc gcacctgcta aaataaacgt tgtgccatcc gtcgcatttg
    18841 taccacgaat cacaatgtca ccaagcgtag gtgctcctat aaatgatcca acggcaacaa
    18901 cacctatcgc aacaaccaag gcaatacgaa tgccaccgat aataaccgaa acagataacg
    18961 gtaattcaat cattcgtagc acttgattgc gtgtcattcc catacctttg ccagcatcct
    19021 taatattcgc atcaacacta gctataccag tataagtgtt ttttataatt ggaagtaacg
    19081 catataaaaa cactgttaaa actactgttt ctgagcctaa gcccatgact aacattaaaa
    19141 tagctaacat tgcaatgact ggaactgttt gaattatatt tgcaattgta attacaaatc
    19201 cagaaagttt tgtgtatctt gcaagcaaga ttcccaatgg aataccaatt aaagctgcaa
    19261 acagcacacc atagacagac attaataagt gtttgaaaaa taagtcccat agatagccaa
    19321 agttcgtaac ataataattg aataattgct gtaataaatt accttccatt acttatgacc
    19381 acctttctgt ttatcaaaat agtggtgttt tttcaaaaat tcttctgcga cgacagcagg
    19441 ttctttacct ttaccatccg cttcataatt caagcgctgc atctctgaag ttgaaatctt
    19501 tcctgtcaac ttattaatcg tcgttttaag ttctgggtgt tggcgtaata attcatttgt
    19561 tgcaacagca ctcgcagcat aaggtgggaa aaattgttta tcatctttaa gtactttcaa
    19621 atcatacgcg gcaattcgac catctgtaga ataacctaat gcaacgtcta acttctctga
    19681 gtttaatgcg tcgtagacta gacctatttg cattggtctc actgtaccaa agtcaaaacc
    19741 atactctttt ttaaatcctt catagccatc gcctttacga ttcatccatg aactatccat
    19801 acctaaacgt aaatctttac tatgctttgc taaatctgaa actgtctcta aatgatattt
    19861 tttagcagtt tccttcgtta ccatgaatgc atacgtattc gcaaaaccat acgaattgaa
    19921 aaatgtttga tcaaatttct ttttaaatcc ttgttgtgtg gctatcattg ctttcttagg
    19981 atctttaatt ggtgcttcct tcaaaactcc cgttaaatct gtgccattat atctaacacc
    20041 tgatatatta gcatccccat taattaaggc attatgttga atcgtacttg accctaaatt
    20101 atttactaat gttggcttta tcttgccatg tgtatcatgc tctattaaca accgtaacat
    20161 atgtgaaata atttgcgatt cgcttgttga taatgctgta attttgacat catttttcgt
    20221 gctcttacta cctagtccgg gcaaactaca tccagataat acggtaagcg ataagacaag
    20281 cacgacaagt atatatttaa ttttcttcat tatcttagcc tcctaattat ccagatactt
    20341 ttaagccttt gggaactacc catttttcaa ctaaagctaa taaggcatca acacctaatg
    20401 ctagtgcagt aacgagtacc gttgcagtta caatcatcag tggatcatat aaatttaaac
    20461 cattgaaaat gaaatcacct aatccacccg cacctacata acttgcaagt gtagcccaac
    20521 taattacata cacagacgac aagcgaatac cgcctagtat aagcggtaag gctaacggca
    20581 attcaacatc cttcatcaat tgaaattgtg tcattcccat actttttcca gcttctttaa
    20641 tgttactatc aatattttga acgccgagta ccgtgttatt taaaataggt aataatacat
    20701 aaataaatag cgctacaatt gcaggcgttt taccaacacc aaaaatcggt atcataatag
    20761 caagtacagc tagtgttgga atagtttgta agacacctgc cacagttaat ataatattgg
    20821 cagttcgctt tgtttttgat aataaaatgc ctataggtac tgcaacaatg atggcaagta
    20881 ataatgccac tatagaaata tagaaatgtt ccagtgtttt cgacatcaac tgtccaccat
    20941 gttcatgtag gaactcgatc atgatttatc atctcctatg tcacgtactt tgacattagt
    21001 agtgtgttgc tcatgcactt ttgaggacgc agtgtcttcc cccacatatt ctgcttgcac
    21061 tgtatcctca ctatcgcccc aaatcgtgtc atatacaata tcaacaacat tggcacgcgt
    21121 aatcagtcct actaaacgct gttgatcatc tacgacaggt acattcctaa cgtttctttt
    21181 taaaatcgta cgtacagaat cttgtaactt agaatcaatt tgaacggtat aaatatgttg
    21241 ttgcatggtt tctcgtaaac ttttgtgtcc acgtataccc tgatttatat cttcaatatc
    21301 taagaatcct agtaaatggt tatccctatc tactacaaaa atagtatcaa cacgtttttg
    21361 tctcataata tgaacggcgt cattcagtgt tgcttctgct tgtatcgtga ttggtttaat
    21421 cattacacct tctacagtct tgtcattggg acggtcttga atcagtctat tttgtcctat
    21481 aaaatcacgt acaaaatcat ttgcgggatg tcttaaaata ttgtccggtg tatcaaattg
    21541 caccacctta ccttctgaca taatacaaat tttgtctgct aatttaatcg cttcatccat
    21601 atcatgtgtt acaaagataa acgtcttgcc taattttcgt tgtaacgttt taactaaatc
    21661 ttgtaacgta tctctcgtaa taggatccaa tgctccaaaa ggttcatcca ttaaaataat
    21721 atcttgttcg gccgcaagtg ctcttacgac accgatacgt tgttgttgcc cacctgatag
    21781 ttctgctgga taacgctcta aaaatgactc cggtaaatcc acaagtttaa ttaattcctt
    21841 tgcacgttta tccttttctt ctttagtcca tttcaacaat ttgggtacca acacaatatt
    21901 ctctttaatc gtcatatgag tcattaagcc aatttgttga ataacatagc caatatttct
    21961 acgtaattcg acaggattca tactccgaac gtctttacca tcaatttcaa tttgtccttc
    22021 tgtcgcttca atcatacggt ttatcattct taaagcagtc gttttgccac ttacactggt
    22081 tccaataaat gcgataaatt ccccagactg aatatctaaa gagatgtcat ctactgcctt
    22141 tttattacca gaatagattt tcgttaaatg cttaatactt aacataatca tttctccttc
    22201 ctcataaaaa atatgacgaa tgatacgcat aaaataaaaa gaccatcatc gcaattgtta
    22261 aacaaataga gtcataagtt aaacaagtac acatcatggt cttgattatg tagaatttta
    22321 tatttttcat ctgtaatgtg aatacttaac agtaaacggc gctatgtatg ttgaatttct
    22381 accgattact ttgtttgcac attcgtatat tcgacattta aaactaacat actctatatg
    22441 atgttattaa acattatcat tttatctttt gcaactaaat agataactca tatttttcaa
    22501 aagcgaattt attatcgctc aattggttgt tttatagcaa aaaagtgtca aaaatgtgaa
    22561 atattattgc aaaagtcgaa tttttgtaat aatgcacacg catccattgt tacaggcgga
    22621 caaacgccca cccgccactc acaaagtcct ccttatattt caatatcttc atcattcatt
    22681 gtagacaccc ttgccttatg ctaacagttc ctactcccaa gcctgtaacg gaattccacc
    22741 gtcaaagtgt tgcccatgat aggcacacct aaaaaacaac caacagcatg agccattggt
    22801 tgtttcgtgt catcttgtat gttgttgtat aaccggtatg cttttactca ttaattactt
    22861 atcatgcatc cctttacctt ctagtatttg gttaatatac ttttcaacac gcttttgtct
    22921 tgtttcactg cgtttagctt gtccaatgtg ataaatgtat tgatgctgtc tgcctggtgt
    22981 caatttataa aatgacgact ctagctgtgg catttcttcg aacttagctt ctaactcttt
    23041 aggaataaca tattcctctg ttttcttcat ttcaactttt ttaccagctt tttcagcttt
    23101 aattgcttca gcaatatagt atttaatttc ttctcgacgt gcttgaatct cttctagatt
    23161 ttcaaatcgt aactgacgag ctgcttgcac cttttcagtc tgttgaatga gtgtatgata
    23221 tttatcctcc aaaatggcac ctttatgaaa taatagtgca acataatttt tgaagccatg
    23281 gataagtact acatttttat tattcaacgt gtaacaagga tgcatccatt tatacgcttc
    23341 ctctaattct gtttcattaa agattaacgc tctcaaatat ttatattctt cttgccattg
    23401 actctcttta tttaaaaatg cctcaacttg ttcattcgat tgtttctcac tcatagtcat
    23461 cacttccatt ttatttttca atacacattg tattatatat cctaatttta taaaaaacta
    23521 attttacaaa tgcttttgca ttcttacaaa aaatgcattt gactattatt ataataagcg
    23581 tataattttt cgcatactat tttttgaatt ttcggcaata acgaaggagt atttatgatt
    23641 aaagacaagc aattgcacaa cgaaaaaatc aatctatccc aattagtctt attagggtta
    23701 ggctctttaa taggatctgg ttggctattt ggtgcgtggg aagcatcatc aatagctgga
    23761 ccagcagcaa tcatatcatg ggttcttgga ttcctagtca ttggaaccat tgcctataac
    23821 tacattgaaa tcggcacaat gtttcctcaa tcaggtggca tgagtaacta tgcccagtat
    23881 acacatggct cattattagg ctttattgct gcttgggcga attgggtgtc tttggtgaca
    23941 ataataccta tcgaagctgt gtcagctgtt caatatatga gttcttggcc gtggcattgg
    24001 gcgaaaccaa tgaaacattt aatggaaaat ggctctatta gcacatacgg attgctagct
    24061 gtatatctca tcattgttat tttttcttta ttaaactatt ggtccgtaaa acttttaaca
    24121 tcatttacga gtttaatttc tgtatttaaa ttaggcgtac ccatgttaac catcatcatg
    24181 ttaatgttat caggattcga cacttcaaat tacggacatt cagcaagcac atttatgcct
    24241 tacggaagtg caccgatttt tgctgcaaca acagcatcag ggattatttt ttcattcaat
    24301 tcattccaga caattattaa tatgggttca gaaattaaaa atcctgaaaa aaatatcgca
    24361 agaggcatcg ctatttcact gtcaatcagt gcagtgttgt acatcatttt acaaagtacg
    24421 tttatcactt ctatgcctca atcaatgtta caacatagtg gatggaatgg catcaacttc
    24481 aattcaccat ttgctgattt agctatctta ttaggaatta attggctcgc aattttacta
    24541 tatattgaag cttttgtatc accattcggt actggcgtct catttgtcgc cgttacaggt
    24601 cgagttttac gagcaatgga gaaaaatgga catatcccta aatttcttgg gaagatgaac
    24661 gaaaaatatc atattccacg tgtagcaatc atctttaatg ccatcattag tatgattatg
    24721 gttacgttat ttagagattg gggtacgcta gcagcagtta tttctactgc aactttagta
    24781 gcctatttaa ctggcccaac gacagtgatt gcattaagaa aaatgggacc aacaatgact
    24841 cgtccattta gagcaaaaat tttaaaagta atggcaccat tatcatttgt attagcttca
    24901 ttagctatat attgggcaat gtggccaaca acggctgaag taattttaat cattatactt
    24961 ggcttaccaa tctacttctt ctatgaatat cgtatgaatt ggcgtaatac aaagaaacaa
    25021 attggtggta gcttatggat tattgtatat ttaatcgtgc tatcaatact gtcatttata
    25081 ggaagcaaag aatttaaagg cttaaatatg attcactatc catttgactt tatcgttatt
    25141 attattgtgg cgcttatctt ctattacatc ggtacaacga gttcatttga aagcgtctat
    25201 ttccgtcgcg caacacgaat caatacaaag atgcgcgagt cactaaataa tgaatcaaag
    25261 tcatcttatt aaatatttta aaatgcagtg ccatatatga tttcaacaca cacattaacc
    25321 aaccattgat ttcaacatct tggttggttt tttattttga aaatgggtta taaataacta
    25381 acataacaag atagcgagag tgatattgta atgaaatgtc actgtagaag aacatcatat
    25441 aatgctgaaa atggtttgag ttaaaacaga tatcaagctc gtctgattca gccacaaaat
    25501 tgtcttgtta tacttgtcac ctatcatcca tagaccgtgg tatgattaaa ttggggatga
    25561 taaaggaggt taataaatat gaagattaat actacaggtg gtcaaattca tggtattaca
    25621 caagatggtt tagatatctt cttaggcatt ccttatgcag aaccaccagt tcatgacaat
    25681 cgctttaaac attctacgtt aaaaacacaa tggtcagagc caattgatgc aactgaaata
    25741 caacccatcc caccgcaacc agacaacaaa ttagaagatt ttttctcctc acaatctgca
    25801 acttttactg aacatgaaga ctgtttatat ttaaatattt ggaaacaaca taatgatcag
    25861 tcgaagaaac ctgtcatcat ttatttttat ggtggtagtt ttgaaaatgg tcatggtaca
    25921 gccgaactct atcaaccggc acatttagta caaaataacg acattatcgt tattacatgc
    25981 aattatcgtt taggcgcatt aggatattta gactggtcat attttaataa aggttttcat
    26041 tccaataatg gactttcaga tcaaatcaat gtcataaaat gggtgcatca atttattgaa
    26101 tccttcggtg gcgacgctaa taacattact ttaatgggtc agtctgcagg cagtatgagc
    26161 atttggactt tacttaaaat acctgacatt gagccatact tccataaagt cgttttgcta
    26221 agtggcgcac tacgattaga cacccttgag agtgcacgca ataaagcaca acatttccaa
    26281 aaattgatgc tcgattattt agatacagat gatgttacgt cattatcgac agatgatatt
    26341 cttatgctga tggcgaagct aaaacaatct cgaggacctt ctaaagggct tgatttaata
    26401 tatgcgccta ttaaaacaga ttatatacaa aataattatc caacaacgaa accaattttt
    26461 gcatgttata caaaagatga aggcgatatt tatattacta gtgaacaaaa aaaattatcg
    26521 ccgcaacgct ttatcgacat tatggaatta aatgatattc ctttaaaata cgaagatgtt
    26581 cagacggcga agcaacaatc tttagcgatt acacattgtt atttcaaaca gccgatgaag
    26641 caatttttac aacaactcaa tatacaagat tcaaacgcac aactatggct tgctgaattt
    26701 gcatggcatg atacttctag tgcacactat cgcagtgctt accatattct agatatggta
    26761 ttttggttcg gaaacttaca aatattagca gcacatcaat atccaacaac tgctcacctt
    26821 aaatttttaa gtcgacaaat gcaaaatgat ttagctaatt ttgctaaaag cggtaaaatg
    26881 ccttggccga tgtatcataa tgaacgtcgc tattatcgca catatcaata acgttatcgc
    26941 atctaaaaaa gcacacctga atttgatagc aggcgtgctt ttcatatgag aattatgatg
    27001 atttaaatgt tttagcataa tgattatttt tcaatgtgaa aacaacgatt aatcctaatg
    27061 agccaatgat ggcactcgta tacgtgatag cttgaacaga aacatgtgtc atgaccaagc
    27121 ctccaataat gcctccaaca ccaataccag cgtttaaact agacatgttc caactcatta
    27181 cttggcttgt gtcgccttca acatgttgaa tcacaccgct ttgcactgct ggattagtac
    27241 tccattgcat gatattccaa ataaatagtc ctgctaacaa tagacctgaa ccaggtaaga
    27301 tcaaattcat aagtaacatc atcacgataa aaatagaaac agaaatcatt aaccaacgct
    27361 tacttgtaat tttatcagag aatataccac ctaatgatgt tccaataacg ccagcgattc
    27421 catttactag aagtgctaat gaaacgaatg acatatcatg accattagat aaaataagtg
    27481 gatttataaa gacgaatgtc actgagtttg caatcaatac taaaaacgta ataattaaat
    27541 attttgctac ttcagcaggt cttaatattt tcgaagtaac atgattttca tgagatggtg
    27601 cctcatgatt cacagggcct cgttgtattt cctgatcctt cggtaaatag atcatcatta
    27661 agaagccaac aataatactc acaataatta agaatagaaa tgtatagcgc caacctacta
    27721 aatcgccgat aaccgttcca attggtacac caaaaacatt ggcaccacta aaccctgtat
    27781 agacaagtcc aatcattttg ccacgatttt taggtgctga taacatcgct gtaatagcta
    27841 atacttttac gataattagt gctgctgctg cagatgagat aattctacca actactaata
    27901 ttgaaaaatt tggcgctaca gcaataatgc cattaccaat gataaatata agtaatgtcc
    27961 ataataatac aggccttgat gaaaaacggt tcgttaattt aacaagaata ggtccacata
    28021 tcgcaaatgt taaagcgtac attgtcacta attgaccaac gacagcttct gatacatgta
    28081 agtcctgact catcaagttc ataattcctg caaccatcat ttcaaccatt cctacgataa
    28141 aaatacttaa aatgaatgta aatagtcgca tgattgacat tttaaaactc ctttcaacaa
    28201 aatttccgat attcatttcc cttagaataa cgatttatta aaaaatgtgt gcaacacaat
    28261 tttatatata accgttctat tatataatat tttgctttaa ttttctgtgc ttaattggaa
    28321 aattattgaa attgtttttc agtatttgaa aaattaacac aagtatgtca tttaataatt
    28381 atgtataatt acttcagttt caacaatgac tccaaaaaag cacgcacgct ttatatgtca
    28441 gaacatataa ctgactacaa agtatgcgtg cctttatgaa tattttcgct gcgctaaata
    28501 ataacattac tatatgtgcg cgtcacttca ttacaactat agcgaagctc taaatcacat
    28561 ccagcaattg ctatcatgac tcactcttaa catccgtatt tcgtgctact aattgatgtc
    28621 ttgcattgaa aaattgacca tagcttaaat atgtcgcaat caaagcagac ataatggtcg
    28681 cagttgtatg aataaacacg actaacaatt gaaatttaat cgcttgtaaa ggtggtacgc
    28741 caccaataat taagcctgtc atcataccag gaatcgacac aagcccatat gttttaaccg
    28801 aatcaattgt tggcactata gctaaacgaa tactttcacg tattgcacct ttagaagcca
    28861 atttagttgt agctgcaagt gataatttag attcaatatt agtaccatct tgtacgaatg
    28921 cacgatctaa attttggtaa gctaaattaa ttgcaatcaa gccattattt gcaagcatgc
    28981 cgccgatagg tataacttca ttggctgtaa attgaatggc ccctgtcgca atagtaccta
    29041 caagcggtaa tgccgttcca acaaagatag ccacaaagga aatcaaaaag acatggtgca
    29101 tgacaggtga tgatcgacta atcgtattcc atgatgcgtt gacaataatg acaaatacac
    29161 ataatactaa tagccatttg tcattaattt taaaaatata gtgcagtaag aatcctaaaa
    29221 tgattaattg tacgaccgca cgtagtgtcg ccacaatcaa atctttaata atatgtagac
    29281 cttctttata agaaataata attggaataa ctaatagcaa tgccgtcagt cctaatgctg
    29341 tattactcat gttggttcaa ctcctctgta ttagaaattt gaccatcaac aatggttata
    29401 cgcttttgaa agtgtcgcat actttggtca tcgctgtggg taatccacat aattgccacg
    29461 ccttgatctg ctaatttaaa aatgatattt tctatttttt ctttattatt aatatctaat
    29521 gcacttgttg attcatctaa tagaaggatg tctggggtat acattaattg gcgtgcaatc
    29581 gcgatccttt gacgctcgcc acctgacata tgttctattt tagaacttaa ttgatagtgt
    29641 cctaatccta catctttaat taattgctta gcacgttttt tatcaaattt atcattacgc
    29701 gcaagagacg gaaaaatcat attgtcttca atcgtttcac caaacaaatc gctttgttgc
    29761 attaaatagc taatgtgtcg gcgtaatatt tctggttcgt aatcatcata tggtttacct
    29821 ttaaagtaaa gcgttccact ggttgggcta attaaattac atattaattt ttgaaatgta
    29881 cttttaccac tacctgaagg accaataatt gcaatggttt cacctttatc tacttttaaa
    29941 ttaatatgat ttagtatgac tctatcactt gcctcataca ctaaatcttt tatctctaac
    30001 aacatattta cccctcctcg tatcatgagt tatttcgata taatagggta atccttttct
    30061 tgaatagcgt gcttacctat aagtcggcct agtcaatgct tattacccat taaaatcgct
    30121 ctaataaaac atatcaaata tagtattccc atttttgaaa aatttagtgc catttcattc
    30181 tggaaaattt ttttaatatt tatgtgaaaa ttgaagtaca ctgcagtgac ttagtctcat
    30241 ctttataaaa ataaaaagcc tttaacatga catctcatgc taactaagca tctaacttag
    30301 ttcttgcatg acatcatatt aaagactttc tttattattt atggatttga ctaccacatg
    30361 atatttttat aagtttcatc attcaatgaa cggacaattt cagtaacaag acggacagaa
    30421 ttttcataat catctacatt gagtaccgat acattagagt gcatgtatcg taacgtgaca
    30481 cctattgtca tcgttggaat accttcattt gcgacatgaa tacttcccgc atctgtacct
    30541 ccacctggtg tcgtatccca ttgtacttcg atgttatgtt tcttagctac atctttaata
    30601 tgctttcgca aaccttggtg cgcaatactt gtagcatcca tcataataac aactggacca
    30661 ccgcctagtt tactatcact cgtttgacct gacatacctg gtgtatcata agcaataccc
    30721 acatcgacgg ctatcgccaa atctggttta attgtattcg ctgccacttt cgcaccacgt
    30781 aacccaactt cttcttgcac tgtggcacca ctgtataagt taatgccaat attttcatct
    30841 tttaaacgtt ttaatacctc aacagctaat gcacagccat agcgattatc aaatgctttc
    30901 gcagttaaat atttatcatt tgtaagcact tcaaattcac tatatggcgt aaccatattg
    30961 cctacttcaa cgccagcttc ttcagcttct tccttgctac taacaccaat atctataaac
    31021 atatttttga tttccattgg ctttttacgt tcttcaggcg ttaagacatg cggcggttta
    31081 gaaccgatga tacctctaat ttctttgcct gaatctgttg taatcgttac tttttgagat
    31141 aacatgactt gattccacca tccatcaact ggcgtaaatg aaataaaacc atgtttatca
    31201 atctttgtta ccataaaacc aacttcatcc atatggccag aaatcataat tgagtattga
    31261 ccattctcag catttttctt tccaaaaatg ccacccaaat tatcttcaat aatttgatca
    31321 ctgacaggct ctatatagtt acgcattgct tctttaactt gcatttcata accagcaatg
    31381 ccgtttacat ctgttaaatg tttaagtaat ttgactgact gattcatgtt attcatcctc
    31441 ctgtatttca acttaaaact attgtatcac taactttcag aaaaacgttc cctaccgctt
    31501 tatcgttttc aggcttaata attttaaaaa ctttgtgaga aatccgcttc aaaaacctca
    31561 tatttacaaa aaaattatca aaatttaaat ttttgaataa catcattttt tagtaaatgt
    31621 tataatttct ttataataca aatatcgaaa gaaggcgact gactttgaaa aaattatgtt
    31681 cattaattgt agtagcatta gtttgtgtca ttgcattatc agcttgtggt aaagaacaaa
    31741 ccaaaacata tgaaggtgat gttagtggta aacatgtact aacttctatc acatataaag
    31801 atgataaagt tttaaaacag tctacaatca acacaattaa atatgacgat ctaggtatgg
    31861 ataaagacga agccaaaaag ttattcgcca aatctgaaag tattttcaaa gaccttaaag
    31921 gcgtaaaata caaagtagac tataaagata aaaaagcaat tgaacactta gacatagatt
    31981 acacagaagt tgacatgaaa aaattaaata aacgtcttgg tgtttcaact aaagaaaata
    32041 aagatattag ttttgaaaaa cttgaaaagc aattaaagca cagaggctta aaagaaaaag
    32101 ataaaatgga cgacaaatag tttataacat aaaaatgccc tcagataaga ctaaggttac
    32161 aaaccttaat tcatattctg agggctttaa tatttgaaat tcttgtgtga ccaacatcca
    32221 ctactaatat aaaattattt gcagtaacgc taaaatccgc tgctttcatt ttcccgaaat
    32281 aaataagtta actaatgagt tttaatttat aatcatgtat cgtttgcaat tcaccatcga
    32341 cttttcgata tacaatatga tcagcagtaa tttctgtagg gcttgatacg ccaacagctg
    32401 ctgcaatatt gaataagcct tcatgcaaac ttgttacata gtttgtgaca cgatattgct
    32461 tttctccaac aatcaatgct ttttcttttt tcgcatctgt cgttgcaaca cctacaggac
    32521 atgtattcat atgacattgt tgactcataa tacaaccgac actaatcatc atgccacgtg
    32581 caatattaac aaaatctgca cctaaaccta gtgcaatcgc aattttatct ggtgtcacta
    32641 acttaccaga tgccgataat ttcactttat ctcgaatacc atatttttct aacatgccag
    32701 acacaatagg tagagctgta aatagcggta agccaacacc atcttgtaat tcttggaatg
    32761 ttgcaccagt accaccttca ccaccgtcaa tcgtaataaa gcttggatac ttatctaatt
    32821 ccaccatcgt acgtacaagt gtttcaattt ctgaaacttt gcttactaca attttgaatc
    32881 ctactggttt ttgacctaat tgctgcaact gatcgacgaa acgaatcaaa tcttcagcat
    32941 tatgaataaa ttcgtaacgg ttaggtgaat tgattgtttt ataaggttca acatttcgga
    33001 ttttagcaat ttcttcgttt accttttcag cttccatatg accaccacga gtcttagcac
    33061 cttgtgccaa cttcagctca aatgcgcgaa cgttagttaa ctgtgcgacc tctttaaata
    33121 aaccttcact aaaattacct tccttatcac gaacaccaaa taaaccggga ccaatttgga
    33181 aaattatatc cccattacct tttaaatgat attctgataa gccaccttca cctgtattca
    33241 tccaagtgcc cgctttagct agacctttag ataaagctgt aatggcattt tttcctaagg
    33301 ctccataact catacccgat tgtcctacga tacgttttaa aataaacgga tgttttaaat
    33361 gttcacctaa ttttattgca tgatcatcac ttaagtaata cggatcaatc tttgtcggta
    33421 cacgatattc ttcacgacta aataaacgct cattcgcgat tttataaatg aatgttgata
    33481 acaatgttgt attatctact gaaatctcat tacgttgcat cggaaacatt gtgttctgta
    33541 tgtaaaagcc gtcttgataa tctttagtag taccgaagct ggtcatacga gagttatatt
    33601 ttccagccaa aacgatattt ttataatcat tacgtgaaaa aggtttccct tcattatccc
    33661 cagaaaataa atactgacgt aattccggtc ccattttttc tgaaatatat ctaatacgtg
    33721 ctagtaaagg ataattcctt aatacactat gttgtgattg tcttttatct ttaattaacc
    33781 aaataagccc gataaccatg accgtaagca ggaagcctat aatgataata ttaactataa
    33841 attgcatgac tgtaagaaac gtcattacaa tccctccccc aaaaaatttc aattcaatat
    33901 ttatgataca ccttacaaaa taaaacacaa tggaagcgct tcattttata aaacaatttt
    33961 atgatatgtt tttcatttta aattttaatg tataaaacat acaatacaaa gtaatatgtg
    34021 ctaaagtatc tatataatac aactatttaa gaggtatact atgtcaaata caaataaaca
    34081 ttacatagaa gaagaatacg ctaccgaaca atcgcgtttt ttcaaacgtg atattggatt
    34141 tattttcctt tatatatttt tggttaacat cttgccaatc attatcgttt ttttaacttt
    34201 tgggttagta gctgtattca ctcaacaagc cccatggggt ttctcttttc ctatttcaat
    34261 gaacattagc tatttactcg cagttattat ttcactagct atttttcgtc ggatgcatga
    34321 agatgcattt gttccaatga tacgtatgca aatcgttgcc agtgctaaat ataaatggca
    34381 tattcttata tcatttacat taagtatcgt actcttttta cttttaaatc atttcgctat
    34441 tttcgcacta aatgtagatt cctttactgt ctttgacatt tacccgctca ttagagaata
    34501 tggttccatc agtttaatag ctatattttc gttaaccatt atattaaata ctttgatttg
    34561 tcaacttatt ttccgtcata ttttaattca agaattaagc agattaatac ctttaaagat
    34621 tacaatagta ttatccgtta tccttgaaac actgtactat tatccataca ttaatacatg
    34681 gtgggaattc attccagcat tgatactagc tagtagtgcg acttacttat atttaaaatc
    34741 aaaccgtaat tttatggtga gctatttcta tcaattagca gttatactcg ttttgcatat
    34801 tttcatgtaa attttttcag agtattatcc attatccata cacatcaaaa agcacactta
    34861 aaagtcagta tcacaactca cttttaagcg tgctttattt agatatactg tcgttctaaa
    34921 aacgagccat atagaatgtt tcatgtccta gcatgaagca catgaatcgc catgctcaat
    34981 atacctttta aaatttaaat attcattaac ttaatatgct ttaattcact tcattaaaga
    35041 ctatttgctg ggcgtggtgt tggttttttc aataaactca taatcattat tacaatgact
    35101 aacataccac aaattagata catccattta aatcctacta aatcggaaat aggtcccatc
    35161 aatgcgccac ctaacgatat acctaagtct gcacaggcaa taaataaacc taacaacata
    35221 ttacgaccta cttttggtaa gacgaaactt aagtatgatg ttaatgttgg gtacaccatt
    35281 gcttgtgtca ttcctattaa tattgcactg ccatagaaaa taattgcacc tacttgcgga
    35341 ccaaatgcta ctacaaatga cgcgattact aataatgata gtacagatac catatattta
    35401 ggatgccaca taccatctga cggaatgtat ttccttaagt aaaatcttgc cacaacaact
    35461 gcgatggcct gtattgtcaa aaagattccc gcattcgcga atcctaaact cactgtgtat
    35521 aacggtacaa atgtactaac tgcaccaaat acaatcgatg caacaatcat gataatacca
    35581 ctgttcaaca actctttatt tttgaaaaat tgcgcaaaaa cagttacagc gttaaacggc
    35641 attttttcaa tcttatctga cgtatcgggt tcctgttcag caaaggtcac gcgatagcca
    35701 aagaatgttg ttgttaatgc gataaagata atgacaattg caaatagtga aatattattt
    35761 gcattccaaa tacctacggc aactaatggt ccgattaagt ttggaatcgt tgaaaatagc
    35821 gagtacaatg atacaccttc agaacgatgt tcctccggta atgcatcaat aatacctagc
    35881 tgtaaagaca tagaaaagaa tgccgtacac acaccttgca taacacgtgc tacgaaataa
    35941 ccttctaagc cagtaaaacc ataaataatt aaagctatgg cattgataat caaaataatt
    36001 cttaatactt taatgggacc gacacgagca ataatttgtc ctgcccatgg tcgaaatacc
    36061 attgctgtta acatgtatgc ccccataacg ataccgataa ccgtattcgt tgcgcctaaa
    36121 tcatgccctc gtaaaggtat aaacacgttt aagattgcat tggcactaaa gaacatcaat
    36181 gttaatatat ataatctcaa aaagggccaa gccattgcac ctttcattat ctcactcctt
    36241 agaatgatag ttgtttttct attaattctc ttgtataagc attgtcactt ttatgcaatg
    36301 cgcttgtcgg aatttgttct tctatttttc cgtttttaaa aataattaat tgatcacata
    36361 aatacgtggc agcttgaata tcatgtgtga taaaaatata actcaactga cgcgtttcac
    36421 gtaaatgaat caataaatct aatatttgtg tttgaattga catgtcgagt gaactaatgg
    36481 cttcatcaaa caaaatatat ttagggttaa tacatattgc gcgcgcaatc gcaacacgct
    36541 gtgcctctcc acctgataac atattaggat atttatccat gtatgcctta gatagaccaa
    36601 cttcttccaa caatgtaatt gcttggactt ccataacatc tttaggttgt ccatcacatt
    36661 gacacatcac ttcaaataag atttctctaa cagtctgaaa tgggtgtaat gatgacgtat
    36721 aatcttgaaa tacagcacca atttgatgac gtctcacttt cttcttatgc atcggttgat
    36781 catttaaggt tacacaacct ttatctggtt tctcaatacc taatatcata cgactcaacg
    36841 tcgatttacc gctaccactt tctccgataa tcgcaatcgt cgcaccgatt ggacactcaa
    36901 atgacacacc tttcacgata ggtgttcgac gacgcttaaa aacatgtgcg ctttgatatg
    36961 acttttcaac atctttaatt ttaatcatgt acatcacccc tcatcacatg tttaaaatga
    37021 tcattaatct ttttcttagt ggataataaa tacttcgtat aaacatgttc tggatgatgc
    37081 aagactgatt cacgcgtacc atgctcaatc agttgaccat ttttcatcac aacaacacgg
    37141 tctgcaatct tgttaataac cgttaaatca tgtgaaatga aaatcatcgc acagtcaaag
    37201 tgttttttta tatctataaa tgcttccagt acatcatatt gtgtaattgt atctaaagcc
    37261 gttgtcggct catcagcaat gattaacttt ggtttcaaag ctaacgctaa agcaatcatc
    37321 aatcgctgta acattcctcc tgataacatg taagggtatg attttaatat acgtttagga
    37381 tctttcaaac ttaaataatc catatattca atcaatgtct tttcaatttc ttgtgtagac
    37441 attgacgtat gtactttcat agtctcaaac atttgtttac cgacagtagt tgatggatca
    37501 aaggcacgac taccttgttg catgaccatc gcaatatctt taccacggta ttttttcaat
    37561 tgtgtttccg ataacgacaa cattgactta ccatcaaagg ttatatcacc tgttacgcgt
    37621 aatcgttctg gatttaaacc tacaatcgat ttgcatgtaa ttgatttacc gctaccactt
    37681 tctccaataa cgcctaaagt ttcaccctta gttaatgtaa aattcacatc actcacgagt
    37741 ggttgatctg tccaggtatc tgtaatcgtc aaatgcttaa ctgttaacaa tgtcatgatt
    37801 gcaccactcc ttttttcaca gaacgaagtt tatctttaga agagatgcgg ggatcaatgg
    37861 caatttgtaa agcatcggat aagaagttaa atgccatgac tataatcact atggcaatac
    37921 ctggcgcaaa catcatttca ggatgtgtaa acatcacttt tctagcttcg ttaagcatca
    37981 tgccccactc tgcagtaggc gctttgacac ctaatcctaa aaatgaaaag ccagatattt
    38041 gcaagatcat tgaacacatc gaactactag agatgatagc aatatctgct aatgttaacg
    38101 gcataatatg tttgtgaata attttcatat cattcatacc gattgtttta gcaaatctta
    38161 catggtcaga agcagtgtac tgcataacac ttgtacgtat aacgcgacag aaccatgccc
    38221 aacgcgtcaa aataaatgcc atgataatat tttcggcacc cataccaaac aatgcaatta
    38281 atgctaacgt tacaacataa cttgggaatg ccaacataac atcacacgca cgcatgatta
    38341 aggcgtcaac aaacccttgg aaatatcctg ataagaatcc taaaatagat ccaataagta
    38401 cagaaacaaa tagtgcaaca aagacatata acaaacttgg tctaatcgca taaattaacc
    38461 tagttaaaat atctctacct aaatggtcag tacctagtag atgttgaaaa ctcatgccag
    38521 caaatttgtt tgctgtatcg atatggttag gatcataaaa tgtcacaagt ggtgctgcta
    38581 atcctaaaaa gacatataat acaataatgc ctaaagcaat cactgcacct ttatcttgta
    38641 ataatcgttt taaaattatc atcgtgcgcc ctcccttaat cttggattta atagcgcatt
    38701 aatgatatct gctaatgtat taaatacaat aaataatacc gctacaatta atacatatgc
    38761 ttgaatgact ggaaaatcat gttcaagtat tgcttttaaa cttaattgac ctaatccagg
    38821 ccatgcaaag atatactcga taacaactag tccacccatt atcattggta tagacataca
    38881 aaagattgat accgcaactt gtaaagcatt acgcaacaca tgcaacatta atgtgataga
    38941 tttcacaccg cttgctctta aataaagtac ataatcttca tttaattgtt ccaccatcga
    39001 gcgtctaaca tttctaaagt aaataccagc ataggcaatc gtaataacga tcactggcaa
    39061 tatgtaactt tctggacctg ttaatccaga agtcggcaat atgtttaact tcactgaaac
    39121 gtaaataata agtattgaag ctatccaata tgatggtaat gcagttagaa agaaagccac
    39181 tgaacgtatc gcacgatcag tgaactttcc tctttttaat gcactaacta cacctaaaat
    39241 aattgatgta atcatcacca taacacttga aatcattgtt aatttcaatg tattcataaa
    39301 tgctggacca atgcgttcag caacaggatc acctgtaatg tagcttgtac caaaattaaa
    39361 ttgcatcgct tcaagtaacc aatttttata ttgaattaat aatggatcat tgaaaccgta
    39421 cttctcattc gtttctgcaa tcaactctgg tgttacattt ggcgttcctt gtgcatgtaa
    39481 aattgtcaca gctggatttt catttgtaat atacgtcaat agaaatgtca taaaacttac
    39541 tacaatcatc aatggaaaca tgagcgcaat acgttttaag ataaatttga acatctaaac
    39601 gctcctttat ttatactgca tttcattgaa tggtaattca tactgtgatt gtgtgaatga
    39661 tactttttct aaatctttcg gcgcaacaac tgtcatacta ccgtgtgaaa tagggataaa
    39721 aataccttca tcatcaattt gtttcaaaat gtttttataa gcgtctgaac gctctttacc
    39781 gttttggatt ttaaatgcgt catcaatgct gttgtatatt ttatctttgt tctcaatgcc
    39841 tgatgttgca ctttcataac catttttcgc tttaaatgct gcaatagtac tttgtggatc
    39901 gtacaataat ccccaagttt ggttgaacat taagtcataa tcaccagaag tacgacgttc
    39961 agcaatttta tctgatgttt cgccattgat gtttaactta atacccattt tcttaaattc
    40021 tgcttgtaag tattctgctt gttctttttg acttgaagaa cctttgtcat agtacattgc
    40081 catttcaagg tttttaccat ctttttgacg aacatcgctg tctttacctt tcttccaacc
    40141 agcttcatct aataatgatt ctgctttttt aaggtcatac ttacgtgttg gcatatcgaa
    40201 attaatgtct gttacatttt tcgcaaataa ttgagttgct ggtttttctt gaccatctaa
    40261 aatttctttg gcaattttat ctctgtttac catatgacca atcgcttgtc tgactgtttt
    40321 gtcactgaca gcgttatctt ttttaccaga attgaccact aacattttcg tattcatagg
    40381 ttgactacgc ttaacttgat agtcacctgt atctttcaat tgttttaaag agtctttgtc
    40441 taagctatct gtacctctat cgtctgtgaa ggcaaagttt gtttcacctt ttttcattga
    40501 taggaatgct gtttcaccag caggcattac ttttgcttgt actttgttaa gtttagactt
    40561 ttcgccccag tattgatcat ttttattaaa gtctgcagac tcatcttttt tgtgttcacc
    40621 taatttaaat ggaccagtac catcgaactt tttaacgcca tcttttgttg tgccgttttt
    40681 aaagtctttt ggagatacaa atacatatgg acgaggcatc gctaattcag ccaatgcagg
    40741 ttgatatgct tctttcaaat ttaattcaac cgtgtactta tctttaactt taacattgtc
    40801 aattaatgtt gaaatcttta accaagaatg caattttttg ttttcttgaa ctgcgtcaat
    40861 atttttctta actgcgtcag catcaaatgg cgtaccatca tggaatttaa cgtcatctct
    40921 caaatggaac gtgtatgtct tcccatcttc tgacacatcc cattttttag ctagtaaagg
    40981 cttaatccca tctttcgtgt tacgtacaag cggctcgtat atcatacttt cagcagacat
    41041 cgatccaccg taaacatgcg gattcatatc cccgatatct ttaaccgtcg tatacgttaa
    41101 ttgcttgttt tcttttttct cctctaaacc tttattaccg ccacaaccag ttaaaattag
    41161 ccctgatgca agtaacattg cactcatttt agttagtttt ctcatttgct tttcctcttt
    41221 ctaaattgat aagttgcatt acttaatcaa atcgtaatga ttatttttct caattatttg
    41281 cttattttta tgtaaatgtc aaacataatt caataatttt tttctattat aaaacttcaa
    41341 acccttatta tgaaagcgtt ctattgattt ccaaaaattt tgtgacgagt gccttatctc
    41401 cttcaaatag atttgtatta aattgagaag atagttgttg atcttgatgc atatccttgt
    41461 acgtcttaca acttgcttca tagcgagtta ggaactgatt aatcattggc gttttgatac
    41521 ctagcatttc cccaatatgc tgaatcatcg ctgttctgta ataatcctca cttggcattc
    41581 ttggaatttg aacaacatcc tgttcatttt tatagacttg cttaaatggt acagctgaaa
    41641 aatcaaagta atgtccgttt tcgtctggct gtgaaaatgg atcaataagg attgcggtat
    41701 atcttacata aagcagatat tcttgtaaga tatctggcaa gatttcgaaa tgctcaatat
    41761 caccttcatc caaagtttca ggacgtactg gataattttc cttcaccata aattgaagca
    41821 ggttgactga cggtactcta aatgcttgta aaatagccat catttccttc cacattaaac
    41881 gcatttcacg gattagtgtc atcgttatcg gtccttcagg aaataactta tacacataaa
    41941 ccggtacatc cgttccttcg aaaatggctt tcaatgaaaa gtcattcata aatagtggtg
    42001 ggtgcacata aagcgagctg tttcgcgttt cagcatgcag tggcgattca acgacttcta
    42061 attgaatctt caactgctca gctaaagcag agattcgttg acacattgtt gagtttgaat
    42121 gtgtcgatcc catgtacaat ttctttttta cacctgttgt caacacatga ttaggtgctt
    42181 ctttatcaac aatacgtgta tcgccaagat aagttgaaaa tgaaatcact tcgatatcct
    42241 gactgaactt agacataaat tgttccacaa tcatttgcga tccaaatgtc ggtgaaatta
    42301 aaatgacatg tttgacactt tgcaaagttt ctaacgacaa ttgctgtagt gtgtcataat
    42361 aagcatctgc tgtgcatgcc atgacaaccg tttcgtattc acccttaacg tttttaacat
    42421 ctttatacaa acgattaatt tcaaacttac cttccagatg ttgatgcgcc tcgttttgta
    42481 ttttgacttc aaattgtttc tcttttttat acgcttgata taagcgtttt gatttttctg
    42541 atgttgaggc acgtccaacc atatcaatct cataatctga ttttaaatag caaatattcg
    42601 ctaattggat tgcgaccgga ccagtgccta tcattaataa tttagacatc cgtaatacct
    42661 acctttatag ctgctttttt ataaagtgct atatcaaaaa tttgctgtgg tctcatatgt
    42721 ttattcacac attgccactt atcttccgct gtttcttgtg acggataatt aaatattgct
    42781 ttgatgccat caccaaagcg cattgcaact acgacatttt catttgttaa atcatataat
    42841 tcttctaaaa tgctgtactt taaaggaatt gtcgagctga atatgatatg cgtcacatct
    42901 ttgatatctt taagttcaga taccttttga tccgtaattg ttatatcttc atttggtgct
    42961 aagacgttaa cgattctgcg tcctaggtca acggcttgtg gatcaatatc aataccgata
    43021 actgaagcac ctgtttcttt tgctacttga attaacgtca ttggatatgc acctgaacca
    43081 actaacaata atttgtcatt gctagctata ctacattgtc caaactcttc atcaatacaa
    43141 tgttctatat tgtcaaagta acctgtggta gacgcctgtc cgtctaatag acgtctagct
    43201 ctaatgactt ccacttgttt aacacactgc gctgtaatat attgcagtga ttttattaat
    43261 tgtgcttttt cttcaacgtc acgccaagca ttaaattgtt gttcataaat aggatttaaa
    43321 ataaattcac tatagtcatc catcaatgtt tctaatactt cgatatattg atcgtcttgt
    43381 aatacacgct cgtaatgcgc ttcaaacttt tctaaatatt gttgtaatat caatttgatt
    43441 tcattattaa agttattcat tgtaggctcc ttattctata tatgcttttc ctgtagctac
    43501 agttgtaacc tgtcctttaa ttgaagtttg atatcccaat tgatgacatc gctttgatgt
    43561 cactaaaata ctgccccctg gctgatgtac tgtaaaatct ttgcatgcgt cattacgttg
    43621 ataattatta aaaaccccaa ttgatgctgt accagaacca cagctatttt cccaaattaa
    43681 actttggatt tctggtatat agattaatgg ctgtaaaaat tgacgttgtt catcaaaaag
    43741 catcatacct actgttttat atttgtgatt ccattgttgc tcacgcacat atgtttcaac
    43801 caaatgttga atttctgttg ttacttgact aactggaatc acataatgta catatgtttc
    43861 ataaataatt tctattgctt tccatgaatg attacccata ttaattgttg ttggcacaac
    43921 acgatgggct tgtggcattt gaacttcata gtattggcag tcatgaattg cgcattgcac
    43981 taaatccgaa cagccagata ccttcacctt aaactgttgg tctttaagca aatgactttc
    44041 ctgcaaatga tgtatatatg acatcgtcgc attaccgcaa aattcattac cgctcataac
    44101 taagtgaaaa tcattgccat catcattttg tgttgattct ataaagccta cctgttcaca
    44161 acatacatgt gttgcggcca tcaactgatt ggcgatagat gcatattcac tagcatcatg
    44221 ttttgaatga acaagtatcg tcatattccc cgaaggatta tacttagaaa attctataac
    44281 ctgccgattc atacgacact cctttagatg tatttttaga gcctctgtaa ttttaattac
    44341 tagaggctct tatgcagttg ttgcgaagca agcaactgta ttcctctagc tcttttacct
    44401 taagtgattt ttcagaagat gaacctaata gtttcccatc tattaggtaa gtcatgctaa
    44461 tccgcaaagt ggaaagcatg acttagtaat cggttgttgc atcaacatgc aaccgtaaac
    44521 cttatttaaa gttgcttctt gtggctctac aagcgaatgc agttgtccgt taactacatt
    44581 cacttagatg cttttactgt tttcacctca actaaaactt caaaaaagtt gcagaaaaca
    44641 aatcgtaact attacgattt gattatatac tatattagtt gagaatcatt gtcaataaag
    44701 gttgtaaatg aaagcacatt tatattttat gaatccctcc ataaacatcg cattctcagc
    44761 acattcaagt ctttttgaaa atttttatca aacaaaaaag ccagcttcaa cgtaaaatac
    44821 gttaaaacta gctttaagca atacatttca tcaaataaac aaatatttat ttttcaaagg
    44881 ttaataattc cccgtcattt ggtacaacaa ctctattacc taagtcgtta gaatttataa
    44941 attcattcag ctcttcacga gataaatgcc aatgattgac cgcttccata ttcacaacaa
    45001 ctatttgagc aagcgacaat gtatgagcga ctttcaatac atcatgttca tccatcacta
    45061 acggcccgcc ttcaaagaat tgatttgcac caccattaag tactatgata tctggatgat
    45121 gcgcttttaa agtcttctca acaccgtcga accaaaccgt atcagcagct aaatataacg
    45181 ttggctcttc agaatgtttc agcactaaac cgtgaacatg tcctgtcatt ttcaagattt
    45241 caccatgccc atgttgagca tctgttttaa tcaactcaat ttctccaatg tgtgtcacat
    45301 cattaaatac cgaaacgttt gtaaagtcat aaccctccac ttcactagcg tctgcttcgt
    45361 tttgtgtata aattttaata tcttttggca atacttcaat cgctttcgga tcaaaatgat
    45421 caatatgcaa atgtgttaaa attactaaat cgatgttgtt aatgatatct tcaattgcta
    45481 atggtagttc tacaagtgga ttgttttcat cttgtctcgg tgaatttgga aatggttcaa
    45541 atgcgccttt gtcacttagc attggatcaa tcaaaacgcg tttacctcca tactcaatca
    45601 cggccgtggc atttcttata tgtctaatat tcatgttaaa tccccctatt tcatatgtct
    45661 gtcattgcga caatatgcat acattgccta cttcatgcca aattattttc cactggatat
    45721 cgattttaaa acaattattc caatgtccgc ttccacgaat catcactcaa catctcacca
    45781 ggccatgtgt aagccataac accaccgtca atgcgaattg tttcaccagt aataaatgaa
    45841 ctctcatcag atgctaaaaa gacaacgagc ttgcctactt cttctggttt gcctaaacgt
    45901 cccagcggtg tcatccattt ctgattttct ctaaacgctt tgccttcctt atcttcgcta
    45961 gtacctgtca gtttatctac taacggggtt tcaattgtac ctggtgcaat tgcattagct
    46021 cgaatgccat ctctaccata ttcgattgca atggatttag taaaattaat cactgcccct
    46081 ttcgcagcat tatatcctga gcgatacaaa tctgctgcct gtcctgaaaa tgaagatgtg
    46141 ttaacaatag agccaccttt agtcatcatt aaaggtaaca tcattttcgt catcaaaaat
    46201 gttccacgca tatctacatt cataatcttg tcatatacat ctgttggata ctcatgaatt
    46261 ctaccagccg cattatccac accggcatta ttaaataaca catcaacatg cccaatctgt
    46321 tctctaattt ctgatgcaaa attgtcaatt tgttgttcgc ttgcaatatc cacaatataa
    46381 gcctttgctt tgtcaccatt acttttaatt ttatcgacag tctccgatac cgcttcagct
    46441 atgtctaccg ccaatacata cgcaccttct tgagctaaag cgattgcaga agcttgtccg
    46501 atacctgtac ttgctcctgt tacgactgca actttatttt ccaaacgttt cattcagtca
    46561 ctcctataca acatatctta aacttacagt ccttattttc cactgttcaa tgcttcataa
    46621 acctaatagc taagtctcca ggcagtctta gctactctct gctatacaat ttctattgaa
    46681 taactcaaaa tatttaacaa tattagattt ttcattttct gaaaattgtt acttttgaga
    46741 gtattttttg ttacacttta aatatttaac aaagggggga atcattgatg acatcaaaac
    46801 atcaacaaaa ggggtctatc gtcaatcgtt tcttgaatag tgttgaaaaa attggaaata
    46861 aattgccaga tcctagcgtt ttattctttt taatgtgtgt gggcttagcc attatgacgt
    46921 gggttatctc attatttaat gtatctgtta agcatccagg tacgcatcaa accatttata
    46981 ttaaaaatat aattagccat gatggattta caatgataat gaatgatacg attaaaaatt
    47041 tctcagagtt cccagcatta ggcttagtac tagcaatgat gattggtata ggtgttgcag
    47101 agaaaacagg atacttcgac aagttaatga tttctgttgt gaatcgcgca ccacgtttct
    47161 taattttacc gactattata ttaattggga ttttaggtag tacagccggc gatgctgcta
    47221 caattatctt gccgccgctc gcagcgatgc tttttattaa aattggctat caccctatcg
    47281 ctggactaac gatggcatat gcttccgctg taggtggctt tgccgcaaat atagttgttg
    47341 gtatgcaaga tgctttagtc tattcattta cagaaccagc aacacgtatc gtttcagatt
    47401 ctattaaaac aaatgttgct atgaactggt actttatcgc agtgagtgtc gttgtattac
    47461 ttcctacgat attgctagtt acaactaaac tgattattcc taggttaggt caatatgatg
    47521 atcgtttaat gcatgatgat catgaagaaa cgtcttcaca tattactgac aaagaagcac
    47581 atgctttaaa atgggcaaat atcagtttca tagtcacaat tattttatta attattacag
    47641 ccattcctga acatagcttt ttaagaaatg ctaaaacagg cagcttacta gacgatgcgc
    47701 cattaattaa cggtgtcgga ttaattattc ttgttgtatt tttagtacct ggattagttt
    47761 atgggatttt aagcaaagaa attaaaaata caaaagactt aggtaaaatg tttggcgatg
    47821 ctgtaggatc aatgggaaca tttatcgtta ttgtgttttt tgcagcacag ctacttgcgt
    47881 atttaaagtg gagtaattta ggaattatcg ctgctgttaa aggtgccaaa ttattagaac
    47941 atcaaaacgg tattgtattg atactaggta ttattgtact gagtgcaatg gtcaacatgt
    48001 taatcggaag cgcatcggct aaatggggta ttttaggacc gatattcgta ccaatgttaa
    48061 tccttatcgg ctttcacccc gcatttacac aagtcattta ccgtgtagga gattcaatta
    48121 ccaatccaat tacaccgatg atgccgtact tacctttact attaacttat gcacaaaaat
    48181 acgataaacg catgaaactc ggagccttac tttctagttt aatgccgtat tcaatcgcgc
    48241 taagtattgc gtggacagta tttgtcatta tttggttctt attaggtatc cctgttggac
    48301 caggtggacc gatattcgtt aaataacagc actcaagggg ataatttaaa atgccactat
    48361 acaagatttg acctgtatag tggcatgatt tgttatgata tagcgttcga gatgaaactc
    48421 tgtatttata tgaacaatta tagtttatca ctaataatat ttagtctgtt ccaaccgtta
    48481 atttggttta caacaaagac aagatcaata atttgttctt catcataaaa tgattttagc
    48541 cggtcaataa tttcaaactt cttaaagtct ttgattgaat ttaacatttc tgcaaattca
    48601 agcgtcactt tttcttgatc agtaaataaa tctaaatgtc taaaactcaa gacttcatca
    48661 attttcattt gtgttacacc caattccttt aattcttttt tatggatatc aacacaataa
    48721 tgacaaccgt taatttttga aattaataac ttaatcaatg cactaagttc atcattaacg
    48781 ggtgattgat gaataaactt ttcaccttgg ctcattacag acattaactc tccattttgt
    48841 ttcttgtaat tatacggcat ttgcaatctc tcctttacat tgtttaatac caatccagtg
    48901 atactacact tcgaacaata ttggatagaa tttgaaattc tgtataacat cttgtcaatc
    48961 tgtgctttag ttaaaacatt tagcgcttat atttaaaaaa tatataaaat atgaaaacag
    49021 attgaaagga tattatacct tgctctaccc ttttttataa aaaatcacac acatttgaga
    49081 aaatttgata atgtcaataa atcacgtcat cgccattgtt attcggtata taatgttttt
    49141 ataatggcca aactattatt tcatttatat taatagggtt cacttgtgtt aatgtactgt
    49201 aaaaaaactg gcgaagtatt ggtgatgatg aagtgattta tattaattgc gaaacacctt
    49261 cgatagcaat gaaatagatt attggaagat taaatcaaaa ataaagaaga ccctagcggc
    49321 cttctttttt gtctaattct tctttgattt cgtcaggtga ttttgttttt attttaatta
    49381 tttccgataa atttttgctt agtattatat cacctacaaa ttgaaagtta ttttcaggcg
    49441 atgcataagc aacaaaatca tcttttttgt tttcgtttat gtatccttca atcactgcac
    49501 ttcccatagg acttgtttcc aactttgtga agtgtacaga tttgatcgta ttcggttctt
    49561 tagtgttatg cttgagataa agtgtaatac gcgcttgttg ttctttataa taaattgctt
    49621 tttgcttttc tttttcatca tatttcattt taaaatgtat gaccgtagcg actatacata
    49681 tacataaaat aacaccaata attataaaaa catgtttttt ggacatttaa ccacctccaa
    49741 cttttagtga cccttctttt tgtttagctc tttttttata tcatcaggcg attttaattt
    49801 ctgtgctggt tttagtaatt cacttaatct ttcactttct atcatagcgc caccaaattg
    49861 ataattatgt tctggtgtag cataagcagt aaaattatct tttttgtttt catttatgta
    49921 accttcaatc atagcacttc ccataggact tgtttccaag ttagtgaaat gtacagattt
    49981 gatcgtgttc ggttctatag tattatgctt gagataaagt gtaatgcgct cttgttgttc
    50041 tttataataa attgattttt gtttttcttt ttcgtcatat ttcattttaa aatagatacc
    50101 tgcaacactg attaatatga ttaacataat attagttaat attaaaattt tctttttcga
    50161 catgtatcac cagctcctaa tgtaaggtgg aaaatgaaaa atttttatag ttctgtaata
    50221 attaatgtta tagtttataa atcatagtac cactatcgtc gtatccaaca acgtgaatat
    50281 tagaattaac tattactgag aattgaacga tatgatattc cgcttctata aaattcgaca
    50341 ccctactaac ataaatatct tcgatagcaa tcgatattaa acatgtacaa atcaagctaa
    50401 atattacgtg aaaatcactg tttagtgttt ttctttttgt ttatttcttc tttaattttt
    50461 tcaggagatt ttaaattttc agcgttcttc aattttccta agacttcatt acttcctgtc
    50521 aatcctccag tgaattgata gttttcttca ggtgacccat atgcaacaaa atcagctttt
    50581 ttgtgttcat tgatgtatcc ttcaattact gcatcgccca taggtcctgt ctttaaactt
    50641 gtgaaatgca cagatttgat ggtattgggt tctttggtat tatgcttgag ataaagtgta
    50701 atgcgctctt gttgtccttt gtagtaaagt gctttttgtt tttctttttc gtcataattt
    50761 catttttaaa taaatgactg atgcaactat acatatacat aaaatgacac caataattat
    50821 aaaaacatgt tttttgttca ttaaagtaac tcctaacttt agtgactcta ctttttattt
    50881 agttcttttt ttatatcatc tggtgatttt aattgatgcg ctggttttag taactcactt
    50941 aatctttcac ttttaattaa atcacctaca aattgaaagt tattttcagg tgatgcatag
    51001 gcaacaaaat catcttcttt attttcattt atgtaacctt caatcacagc acttcccata
    51061 ggacttgttt ccaagtttgt gaaatgtaca gatttgattg tgttcggttc tatagtatta
    51121 tgcttaagat aaagtgtaat gcgcgcttgt tgttctttat aataaattga tttttgtttt
    51181 tctttttcat catatttcat tttaaaatag atacttgcaa cactaattaa tatgattaat
    51241 ataatactag ttaacattaa aattttcttt ttcaacatat atcaccagct cctaaaatgt
    51301 ggtgggattt tatacagttt acatgtttat gatactacat aacacgctct tacaaagaaa
    51361 aactataatt ttaatctagg tcaaggaaaa gcaattaaaa gtgttgatat aaagttcatc
    51421 taagatcatc ggaatattgc ggttatgaag tggttaacag ataaattcag tggaaattgt
    51481 actaaaaatt caacttttca tttttatgtg taagtatgat taacttgaaa gcaaaattaa
    51541 aacgactgca tagttttagc ggtcgttctt tttagactct aattcctttt ttatctcatc
    51601 aggtgatttg gtttgatgtg ctggttttaa taacgtgctt aaatttttac ttttgatcaa
    51661 acttccacca aattgataat gatgttctgg agacccatat gcaacaaaat cagctttttt
    51721 gttttcattg atgtaacctt caattacggc atcgcccata ggtcctgtct ttaaacttgt
    51781 gaaatgtaca gatttgatcg tgttcggttc tttagtgtta tgcttgagat agagtgtaat
    51841 acgctcttgt tgttctttat aataaattga tttttgcttt tctttttcgt catatttcac
    51901 ttttaaataa atgactgtag caactataca tatacataaa ataacaccaa taattataaa
    51961 aacatgtttt ttggtcatta aagcaactcc taaaatgtgg tggaaaatga aaaagttttt
    52021 atagttctgt aattatgaac attatagatt ataaatcata gcactaatat cgtcgccttc
    52081 actcactcga ttgttaaaat caccagttac tgagaattgt gcaaggaata atttcctatc
    52141 atgcttttat atcattattg ccttactaat attaacaaaa ccgatagcta ttacattaaa
    52201 tgcctacctc ccacacctca aacacctttt tatacaggac gcactgtaat ttcattcacg
    52261 ttaacgtgct ttggttgtgt taatgcatat aatactgctt ctgcaatatc ttgtggctca
    52321 agttttttac gatctgttgg attgtatgtg gcagttattg ctgtatcaac cattcctgga
    52381 gaaatgcttg ttactttaac acctgtcttt gccaactctt tttctaatcc ttgagtaata
    52441 gtgtgaactg ccgctttcgt cgcactataa atcgtactgc ttttcgttac ttcaaagcca
    52501 gaaatagatg caatgttaat aagatggcca cttgattgtt ctaacatagt tggtaatgca
    52561 gcctttgccg tgtataaagt gcctttgata ttaacatcaa tcatactatc ccattcatct
    52621 acttgataat cagtaatctt agacgacaac atttgccccg cactattgat gacaatatcc
    52681 aaaccaccga atgtttgttg tgccattttt atcaattcat cgacttcttc ttttttcgtt
    52741 acatcagttg gcactacctt cacactatct tgagctaatt gattcgccac attttgtagt
    52801 ttatctttat ttctaccggc taagacaact ttcgcccctt cttcatgtag taatgttgca
    52861 attgcttctc caataccact acctgcacct gtaactactg ctattttatc tgttaatact
    52921 gtcataatga tcgactcctt tgattctttt tattttttta gggtacatca taagtacata
    52981 ttactattta tacttttaaa aagcgtatca caattcatat tctggttata ctaactcgat
    53041 ttatttacac tgataaagta catttttaag ttattcaaaa tatacaaata atcgttgttc
    53101 atatttttta tttttaaaaa tgttgaaaaa ttgtcaattc tttattgtaa aagtcgaaag
    53161 ttagtactaa agttataagt attagtaaac aatggcaagg ctgttagaaa tgggggagtt
    53221 attgtgacat atcatgagcg tgtgttagca ctgagagcag aaagtaaaag aactgcattt
    53281 gattttagat tcgaggattt atttagcaaa gaagaatggc taagtatgtc gcttgcagaa
    53341 agacaaaaag ccgagaaagc atttcgacac gaaatcaaaa aattagatga tgtaagaatg
    53401 cccttctcaa gtgcacatga tgcccaagtg aaactgtata atgttgtgta ttcatacaat
    53461 ggcattaaac gtaatttcaa acaagttgaa aatgaaggat tctaatatca tttcgtttat
    53521 atatcgtgga catgatacag ttatatataa tcttgtaggt aatcatacta aaattcaata
    53581 tagacatgac taaagccaca tccaatatag gacgtggctt ttagtatcgt tccagatttg
    53641 tggggagtta gggtttactt aattaaaggg gtctgccctc aattgctcac caacgagggc
    53701 aagttacatc aatcacactt caattgccgc cgactagggg tagtaatcat tggcaataag
    53761 aactagttag tgactaacga tttacgttcc atagcaaagt gatacaaacg ctcagcatca
    53821 atgtaaggca tagaatcaat agttaggtaa ccatctttga tgtcaccaat taatccttga
    53881 tttacaccag tgttcacgta aatatcatag ctacttacgt ctgagaaaat aagcgcttca
    53941 atctcttttc tcataatcat cactccaatg tttatatatt atttatataa actctctcgt
    54001 ctctctctat ttattaactt cttacaagtc
//
LOCUS       DAKSQE010000004        51691 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.004, whole genome shotgun sequence.
ACCESSION   DAKSQE010000004 DAKSQE010000000
VERSION     DAKSQE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 51691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51691
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.004"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(62..499)
                     /locus_tag="O0V33_000164"
     CDS             complement(62..499)
                     /locus_tag="O0V33_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCX8974860.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEEVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQVP
                     SSPSYALFKGRDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
     gene            complement(515..1639)
                     /locus_tag="O0V33_000165"
     CDS             complement(515..1639)
                     /locus_tag="O0V33_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HCX8974861.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEENQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEDILPDIKAAFSGESQVLESGKDPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTKHDNIVFMRKWLDLGN
                     RYGNIEEVMDGVLEEVLATYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPAVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMTNRTI"
     gene            complement(1677..1928)
                     /locus_tag="O0V33_000166"
     CDS             complement(1677..1928)
                     /locus_tag="O0V33_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HCX8974862.1"
                     /translation="MQIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            complement(1940..2134)
                     /locus_tag="O0V33_000167"
     CDS             complement(1940..2134)
                     /locus_tag="O0V33_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HCX8974863.1"
                     /translation="MSRILTYSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            2379..3083
                     /locus_tag="O0V33_000168"
     CDS             2379..3083
                     /locus_tag="O0V33_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HCX8974864.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTAIFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            3324..3725
                     /locus_tag="O0V33_000169"
     CDS             3324..3725
                     /locus_tag="O0V33_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCX8974865.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDEQHYTYTHELGEMDNHTA
                     EWAACIYALEHARELNVSNALLYTDSKLIADSVNAGYVKNAKFKPYFDQLEIFEQDFD
                     LLFVKWIPREQNKEANQHAQQALYKFIKKNK"
     gene            complement(3768..35645)
                     /gene="ebh"
                     /locus_tag="O0V33_000170"
     CDS             complement(3768..35645)
                     /gene="ebh"
                     /locus_tag="O0V33_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
                     /protein_id="HCX8974866.1"
                     /translation="MNYRDKIQKFSIRKYTVGTFSTVIATLVFLGLNTSQAQAAETNQ
                     PASALKQKQQNGDTQTENREVEVQNSQNGQSLSAPIENEQPNNNQTNHVDASAVQSST
                     TEHDQPVSQNEQAKKDTAAATPTQSAKAASKHEQSESRAANKKENDNKATHVESHEAN
                     VVTASDSSDSGNVQHDRNELQAFFDANYHDYRFIDRENADSGTFNYVKGIFDKINTLL
                     GSNDPINNKDLQLAYKELEQAVALIRTMPQRQQTSRRSSRIQTRSIESRAAEPRSVSD
                     YQNANSSYYVENANDGSGYPVGTYINASSKGAPYNLPTTPWNTLKASDAKEIALITAK
                     QTGDGYQWVIKFNKGHAPHENMIYWFALPAGQTPVGRTEFVTVNADGTNVQWSNGAGA
                     GANKPLPEMWPYGVNDSRSWDYKIRNRSGQVIYDWPTVHINSLKDLARASDYFSEAGA
                     TPATKAFGRQTFEYINGERPTESPGVPKVYTFIGKGDASYTISFKTQGPTIDKLYYAA
                     GGRALEYNQLFMYSQLYVESTQDYQQRLNGLRQVVNRTYRIGTTKRVEVSQGNVQTKK
                     VLESTNLNIDDFMDDPLSYVKTPSNKVLGFYPTSANTNAFRPGGVNPLNEYQLSQLFT
                     DDKLQQAARTGSPIRLMIGFDYPDAFGNGETLVPVNLTVLPEIQHNIKFFKNDDGQNI
                     ADKPASKQAGHPVFYVYAGNQGNASVNLGGSVTSIQPLRINLTSNENFTDKDWQITGI
                     PRTLHIENSTNRTNNARERNIELVGNLLPGDYFGTIRFGRKEQLFEIRVKPHTPRITT
                     TAEELRGTALQKVPVTVTDIPLDPSALVYLVIPTSQTRDGGSEADQIPSGYTKIATGT
                     PDGVHSTITIRPEDYVVFIPPVGNQIRALIFYNNVVASNMSNAVTILPDDIPPTINNP
                     VGLNAKYYRGDEVSFTMGVSDRHSGLKSTTITTLPSGWTSNLTKSDKKNGSLAISGRV
                     SMNQAYNSDITFKVSATDNVNNTTNDSQSKHVTVHVGKISDDAHPIVLGNSEKVVVVN
                     PTALTGDEKQRITTAFMNKNQNIRGYLASSNPVTVDNHGNVTLQYRDGSSTTLDATNV
                     MTYEPVVKPEYQTANAAKTATVTIAKGQSFNIGDIKQYFTLSNGQAIPSSSFTNITSD
                     RTIPTAQEVSQMNAGTQLYHIVATNAYHKDTEDFYITLKIIDVKQPEGDQRVYRMSTY
                     DITTDEISKVKQAFINANRDAISFAEGDISVTNTPNGSNVSTITVNINKGRLTKSFTS
                     NLNNMNFLRWVNFPQDYTVTWTNAKIANRPTDGGLSWSDDHKSLIYRYDATLGTQITT
                     NDILTLLKATTTVPGLRNNIAGNEKAQAEAGGRPNYKTTGYSQSNPTSDGQRQFTLNG
                     QVIQIMDIINPSNGFGGQPVTNSNVRANHSNSTVVSVNESAANGAGAFTIDHVVKNNS
                     THNAADAVYKAQLYLSPYGPKQYVEHLNQNTDNTNEAINIYFVPSDLVNPTISVGNYT
                     NHQVFSGETFTNTITANDNFGVQSVTVPTTSQLTGTVDNNHQHVSATAPNVTSTTNKT
                     INLVATDTSGNTATTSFNVTIKPLRDKYRVGTSSTAANPVRIANISNNATVSQADQTA
                     IINSLTFTSNAPNRNYATASANEITSKTVSNVSRTGNNAQVTVTVTYQDGTTSTVTVP
                     VKRVIPEIVAHSHYTVQGQDFPTGNGASASDYFKLSNGSAIPDATITWVSGQAPNKNN
                     TTIGQDINVTAHILIDGETTPITKTATYKVVRTVPKQVFETARGVLYPGVSDMYDAKQ
                     YVKPVNNSWSTNAQHMNFQFTNSYGPSKDVVGISTRDIRVTYDNHQTQIIKILSKVKP
                     DPPRIDGNSVTYKAGLTNQQIKINNVLSSSSIKLFKADNTPLTITNTTYGSGNTAVVT
                     VSDALPNGEIKARSSISMNNVTYTTQDEHGRAIDVTRNESVDSNDSASVHVTPQLQAT
                     TEGAVFIKGGDGFDFGHVERFIQNPPHGATVAWHDNPDTWKNTVGNTHKTAVVTLPNG
                     QGTRNVEVPVKVYPVANAKAPSRDVKGQNLTNGTDAINYITFDPNTNTNGITAVWANR
                     QQPNNQQAGVQHLNVDVTYPGISAAKRVPVTVNVYQFEFPQTSYTTTVGGTLASGTQA
                     SGYAHIQNATGLPTDGFTYKWNNAATGTNDANWAAMNKPNTAQVINAKYDVIYNGHTF
                     ATSLPAKFVVKDVQPAKPTVTETAAGVITIAPGANQTVNTHAGNVTTYADKLVIKRNG
                     NVVTTFTRHNNTSPWVKEASAANVAGIAGTNNGITVAAGTFNPADTIQAVATQGSGET
                     ISDEQRSDDFTVVAPQPNQATTKIWQNGHVDITPNNPSGHLINPTQAMDIAYTEKVGN
                     GAEHSKTLNAVRGQNNQWTIANKPDYVTLDAHTGKVTFNANTIKPNSAITITPKAGTG
                     HSASSNPSTLTAPAAHTVNTTEIVKDYGSNVTAAEINNAVQVANKRTATIKNGTAMPT
                     NLAGGSTTTIPVTVTYNDSSTEEVQESIFTKADKRELITAKNHLDDPVSTDGKKPGTI
                     TQYNNAIHNAQQQINTAKTEAQQVINDERATPQQVNAALSKVQAAQTKINEAKALLQN
                     KEDNSQLVTSKNNLQSSVNQVPSTTGMTQQSIDNYNAKKREAESEITAAQRVIDNGDA
                     TAQQISDEKHRVDNALTALNQAKQNLTADTHELEQAVHQLNRTGTTTGKKPASITAYN
                     NSIRALQSDLTSAKNSANAIIQKPIRSVQEVQTALTNVNRVNDRLTLAINQLVPLADN
                     SALRTAKTKLDEEINKSVTTDGMTQSSIQAYENAKRAGQTESTNAQNVINNGDATDQQ
                     IAAKKTKVEEKYNGLKQAIAGLTPDLAPLQAAKTQLQNDIDQPTSTTGMTSASVAAFN
                     DKLSAARTKIQEIDRVLASHPDVATIRQNVTAANATKTALDQARNGLTVDKAPLENAK
                     NQLQQSIDTQTSTTGMTQDSINAYNAKLTAARNKIQQINQVLAGSPTVDQINTNTSAA
                     NQAKSDLDHARQALTPDKAPLQTAKTQLEQSINQPTDTTGMTTASLNAYNQKLQAARQ
                     KLTEINQVLNGNPTVQNINDKVAEANQAKDQLNTARQGLTLDRQPAFTTLHGASNLNQ
                     AQQNNFTQQINAAPNHAALETIKSNITALNNAMTKLKDSVADNNSIKSGQNYTDATTA
                     NKQAYDNAVNAAKGVIGETTNPTMDVNTVNQKAETVKSTKGALDGQQNLQRAKTEATN
                     AITHASDLNQTQKNALTQQVNNAQNVQAVNDIKQTTQSLNTAMTGLKRGVANHNQVVQ
                     SDNYVNADTNKKNDYNNAYNHANDIINGNAQHPVITPSDVNNALSNVTSKEQALNGEA
                     KLNAAKQEANTALGQLNNLNNAQRQNLQSQINGAHQIETVNTIKQNATNLNSAMGNLR
                     QAVADKEQVKRTEDYADADTAKQNAYNSAVSSAETIINQTTNPTMSVNDVNSATSAVT
                     TNKNALNGDEKLAQSKTDAASAIDALPHLNNAQKADVKSKINAASNIAGVNTVKQQGT
                     DLNTAMGNLQGAINDEQTTLNSQNYQDATPSKKTAYTNAVQAAKDILNKSNGQNKTKD
                     QVTEAMNQVNSAKNNLDGTRLLDQAKQTAKQQLNNMTHLTTAQKTHLTNQINSGTTVA
                     GVHTAQSNANTLDQAMNTLRQSIANKDATKASEDYVDANNDKQTAYNNAVAAAETIIN
                     ANSNPEMNPSTITQKAEQVNSSKTALNGDENLATAKLNAKTYLNTLTSITDAQKNNLI
                     SQISSATRVSSVDTVKQNAQHLDQAMASLQNGINNESQVKSSEKYRDADTNKQQEYDN
                     AITAAKAILNKQHGPNTAQNAVEAALQRVKTAKNALNGDAKLIAAQNAAKQHLGTLTH
                     ITTAQRNDLTNQISQATNLAGVESVKQSANSLDGAMGNLQTAINDKSGTLASQNFLDA
                     DEQKRNAYNQAVSNAETILNKQTGPNTAKTAVEQALNNVNSAKHALNGTQNLNNAKQA
                     AITAINGASDLNQHQKDALKAQANGAQRVSNAQDVQRNATELNTAMGQLQHAIADKTT
                     TLASSKFVNADSTKQNVYTTKVTNAEHIISGTPTVVTTPSEVTAAANQVNSAKQELNG
                     DERLRVAKQNANTAIDALTQLNTPQKAKLKEQVGQANRLEDVQSVQTNGQSLNNAMKG
                     LRDSIANETTVKASQNYTDASPNNQSTYNSAVSNAKGIINQTNNPTMDASAITQATTQ
                     VNNAKNGLNGAENLRNAQNTAKQNLNTLSHLTNNQKSAISSQIDRAGHVSEVTAAKNA
                     ATELNTQMGNLEQAIHDQNTVKQGVNFTDADKAKRDAYTNAVSRAETILNKTQGANTP
                     KQDVEAAIQSVTSAKNALNGDQNVTNAKNAAKHALNNLTSINNAQKRDLTTKIDQATT
                     VSGVEAVSNTGTQLNTAMANLQNGINDKTNTLASENYHDADSDKKTAYTQAVTNAENI
                     LNKNSGSNLDKAAVENALSQVTNAKGALNGNHNLEQAKSNANTTINGLQHLTTAQKDK
                     LKQQVQQAQNVAGVDTVKSSANTLNGAMGTLRNSIQDNAATKNGQNYLDATESNKTNY
                     NNAVDSANGVINATSNPNMDANAINQIATQVTSTKNALDGTHNLTQAKQTATNAIDGA
                     TNLNKAQKDALKAQVTSAQRVANVTSIQQTANELNTAMGQLQHGIDDENATKQTQKYR
                     DAEQSKKTAYDQAVAAAKAILNKQTGSNSDKAAVDRALQQVTSTKDALNGDAKLAEAK
                     AAAKQNLGTLNHITNAQRTDLEGQINQATTVDGVNTVKTNANTLDGAMNSLQGSINDK
                     DATLRNQNYLDADESKRNAYTQAVTAAEGILNKQTGGNTSKADVDNALNAVTRAKAAL
                     NGADNLRNAKTSATNTINGLPHLTQLQKDNLKHQVEQAQNVAGVNGVKDKGNTLNTAM
                     GALRTSIQNDNTTKTSQNYLDASDINKNNYNTAVNNANGVINATNNPNMDANAINGMA
                     NQVNTTKAALNGVQNLAQAKTNATNTINNAHDLNQKQKDALKTQVNNAQRVSDANNVQ
                     HTATELNGAMTVLKAAIADKERTKASGNYVNADQEKRQAYDSKVTNAENIINGTPNAT
                     LTVNDVNSATSQVNAAKTVLNGDNNLRVAKEHANNTIDGLAQLNNAQKAKLKEQVQSA
                     TTLDGVQTVKNSSQTLNTAMKGLRDSIANEATIKAGQNYTDASPNNRNEYDSAVTAAK
                     AIINQTSNPTMEPNTITQATSQVTTKEQALNGAQNLAQAKTTAKNNLNNLTSINNAQK
                     DALTRSIDGATTVAGVNQETVKATELNNAMHSLQNGINDETQTKQTQKYLDAEPSKKS
                     AYDQAVNAAKAILTKASGQNVDKAAVEQALQNVNSTKTALNGDAKLNEAKAAAKQTLG
                     TLTHINNAQRTALDNEITQATNVEGVNTVKAKAQQLDGAMGQLETSIRDKDTTLQSQN
                     YQDADDAKRTAYSQAVNAAATILNKTSGGNTPKADVERAMQAVTQANTALNGIQNLER
                     AKQAANTAITNASDLNTKQKEALKAQVTSAGRVSVANGVEHTATELNTAMTALKRAIA
                     DKADTKASGNYVNADANKRQAYDEKVTAAENIVSGTPTPTLTPSDVTNAATQVTNAKT
                     QLNGNHNLEVAKQNANTAIDGLTSLNGPQKAKLKEQVGQATTLPNVQTVRDNAQTLNT
                     AMKGLRDSIANEATIKAEQNYTDASPNNRSEYDSAVTAAKAIIGQTSSPTMNAQEINQ
                     AKDQVTAKQQALNGQENLTNAQINAKQHLNGLSDLTNAQKDAAKRQIEGATHVSEVTQ
                     AQNNADALNTAMTNLKNGIQDQNTIKQGVNFTDADEAKRNAYTNAVTQAEQILNKVQG
                     PNTAKDNVESALQNVQRAKNDLNGNQNVANAKTTAKNALNNLTSINNAQNEALKSQID
                     SATTVAGVNQVSATASELNTAMSNLQNGINDEAATKAAQKYTDADSDKQTAYNDAVTA
                     AKTLLDKTAGTNDNKAAVEQALQRVNTAKTALNGDARLNQAKNTAKQQLATMSHLTDA
                     QKANLTSQIERGTTVAGVQGIQANAGTLNEAMNQLRQSIASKDATKASEDYHDANTDL
                     QNAYNDAVTNAEGIISATNNPEMNPDTINQKASQVNSAKSALNGDEKLAAAKQTAKSD
                     IGRLTDLNNAQRTAANAEVDQAPNLAAVTAAKNKATSLNTAMGNLKHALAEKDNTKRS
                     VNYTDADQPKQQAYDTAVTQAEAITNANGSNANETQVQAALNQLNQAKNDLNGDNKVA
                     QAKETAKRALASYSNLNNAQSTAATSQIDNATTVAGVTAAQNTANELNTAMGQLQNGI
                     NDQNTVKQQVNFTDADQGKKDAYTNAVTNAQGILDKAHGQNMTKAQVEAALNQVTTAK
                     NALNGDANVRQAKSDAKANLGTLTHLNNAQKQDLTSQIEGATTVNGVNGVKTKAQDLD
                     GAMQRLQSAIANKDQTKASENYIDADPTNKTAFDNAITQAESYLNKDHGANKDKQAVE
                     QAIQSVTSTENALNGDANLQRAKTEATQAIDNLTHLNTPQKTALKQQVNAAQRVSGVT
                     DLKNSATSLNNAMDQLKQAIADHDTIVAGGNYTNASPDKQGAYTDAYNAAKNIVNGSP
                     NVITNAADVTAATQRVNNAETGLNGDTNLATAKQQAKDALRQMTHLSDAQKQSITVQI
                     DNATQVTGVQSVKDNATNLDNAMNQLRNSIANKDEVKASQPYVDADRDKQNAYNTAVT
                     SAENIINATSQPTLDPSAVTQAANQVNTNKTALNGAQNLANKKQETTANINQLSHLNN
                     AQKQDLNTQVTNAPNISTVSQVKTKAEQLDQAMERLINGIQDKDQVKQSVNFTDADPE
                     KQTAYNNAVTAAENIINQANGTNANQSQVEAALSTVTTTKQALNGDRKVTDAKNNANQ
                     TLSTLDNLNNAQKGAVTGNINQAHTVAEVTQAIQTAQELNTAMGNLKNSLNDKDTTLG
                     SQNFADADPEKKNAYNEAVRNAENILNKSTGTNVSKDQVEAAMNQVNTTKAALNGTQN
                     LEKAKQHANTAIDGLSHLTNAQKEALKQLVQQSTTVAEAQGNEQKANNVDAAMDKLRQ
                     SIADNATTKQNQNYTDSSPNKKDAYNNAVTTAQGIIDQTTSPTLDPTVINQAAGQVST
                     TKNALNGNENLEAAKQQATQSLGSLDNLNNAQKQAVTDQINGAHTVDEANQIKQNAQN
                     LNTAMGNLKQAIADKDATKATVNFTDADQAKQQAYNTAVTNAENIISKANGGNATQTE
                     VEQAIQQVNAAKQALNGNANVQHAKDEATALINSSNDLNQAQKDALKQQVQNATTVAG
                     VNNVKQTAQELNNAMTQLKQGIADKEQTKADGNFVNADPDKQNAYKQAVAKAEALISG
                     TPDVVVTPSEITAALNKVTQAKNDLNGNTNLATTKQNVQHAIDQLPNLNQAQRDEYSK
                     QITQATLVPNVNAIQQAATTLNDVMTQLKQGIANKAQIKGSENYHDADTDKQTAYDNA
                     VTKAEELLKQTTNPTMDPNTIQQALTKVNDTNQALNGNQKLADAKQAAKTNLGTLDHL
                     NDAQKQALTTQVEQAPDIATVNNVKQNAQNLNNAMTNLSNALQDKTETLNSINFTDAD
                     QAKKDAYTNAVAHAEGILSKANGSNASQTEVEQAMQRVNEAKQALNGNDNVQRAKDAA
                     KQVITNANDLNQAQKDALKQQVDAAQTVANVNTIKQTAQDLNQAMTQLKQGIADKDQT
                     KANGNFVNADTDKQNAYNNAVAHAEQIISGTPNANVDPQQVAQALQQVTQAKGDLNGN
                     HNLQVAKDNANTAIDQLPNLNQTQKTALKDQVSHAELVTGVNAIKQNADALNNAMGTL
                     KQQIQANSQVPQSVDFTQADQDKQQAYNNAANQAQQIANGTPTPVLTPDAVTQAVTTM
                     NQAKDALNGDEKLAQAKQDAIANLDTLRDLNQPQRDALRNQINQAQALATVEQTKQNA
                     QNVNTAMSNLKQGIANKDTVKASENYHDADADKQTAYTNAVSQAEGIINQTTNPMLNP
                     DDITRALTQVTDAKNGLNGEAKLATEKQNAKDAVNAMTHLNDAQKQALKGQIDQSPEI
                     AIVNQVKQTATSLDHAMDQLSQAINDKAQTLADGNYLNADPDKQNAYKQAVAKAEALL
                     NKQSGTNEVQAQVESITNEVNAAKQALNGNDNLANAKQQAKQQLANLTHLNDAQKQSF
                     ESQITQAPLVTDVTTINQKAQALDHAMELLRNSVADNQATLASEDYHDATAQRQNDYN
                     QAVTATNNIINQTTSPTMNPDEVNRATTQVNNTKVALDGDENLVAAKQQANNRLDQLD
                     HLNNAQKQQLQSQITQSSDIAAVNGHKQTAESLNTAMGNLINAIADHQTVEQLGNFVN
                     ADTDKQTAYTTAVNEAEAMINKQTGQNANQTEVEQAITKVQTTLQALNGDHNLQVAKA
                     NATQAIDALTSLNDPQKTALKDQVTAATLVTAVHQIEQNANTLNQAMHGLRESIQDNA
                     ATKANSKYINEDQSEQQNYDQAVQAANNIINEQTATLDNNAINQAATTVNTTKAALHG
                     DVKLQNDKDHAKQTVSQLTHLNNAQKHMEDTLIDSETTRTAVNHDLTEAQALDQLMDA
                     LQQSIADKEATRASSAYVNAEPNKKQSYDEAVQNAESIIAGLNNPTINKGNVTSATQA
                     VTSSKNALDGVERLAQDKQTAGNSLNHLDQLTPAQQQALENQINNATTRDKVAEIIAQ
                     AQALNEAMKALKESIKDQPQTEASSKFINEDQAQKDAYTQAVQHAKDLINKTTDPTLA
                     KSIIDQATQAVTDAKNNLHGDQKLDQDKQRATETLNNLSNLNTPQRQALENQINNAAT
                     RGEVAQKLTEAEALNQAMEALRNSIQDQQQTEAGSKFINEDKPQKDAYQAAVQNAKDL
                     INQTNNPTLDKAQVEQLTQAVNQAKDNLHGDQKLADDKQHAVTDLNQLNGLNNPQRQA
                     LESQINNAATRDEVAQKLAEAKALDQAMQALRNSIQDQQQTESGSKFINEDKPQKDAY
                     QAAVQNAKDLINQTGNPTLDKSQVEQLTQAVTTAKDNLHGDQKLARDQLQAVTTVNAL
                     PNLNHAQQQALTDAINAAPTRTEVAQHVQTATELDHAMETLKNKVDQVNTDKAQPNYT
                     EASTDKKEAVDQALQAAESITDPTNGSNANKDAVDQALTKLQEKVNELNGNERVAEAK
                     TQAKQTIDQLTHLNADQIATAKQNIDQATKLQPIAELVDQATQLNQSMDQLQQAVNEH
                     ANVEQTVDYTQADLDKQNAYKQAIADAENVLKQNANKQQVDQALQNILNAKQALNGDE
                     RVALAKTNGKHDIEQLNALNNAQQDGFKGRIDQSNDLNQIQQIVDEAKALNRVMDQLS
                     QGITGNEGRTKGSTNYVNADTQVKQVYDEAVDKAKQALDKSTGQNLTAEQVIKLNDAV
                     TAAKKALNGEEKLNNRKSEALQRLDQLTHLNNAQRQLAIQQINNAETLNKASRAINRA
                     TKLDNAMGAVQQYIDEQHLGVISSTNYINADDNLKANYDNAIANAAHELDKVQGNAIA
                     KAEAEQLKQNIIDAQNALNGDQNLANAKDKANAFVNSLNGLNQQQQHLAHNAINNADT
                     VSDVTDIVNNQIDLNDAMETLKHLVDNEIPNAEQTVNYQNAEDNAKTNFNDAKRLANT
                     LLNSDNTNVNDINGAIQTVNDAIQNLNGDQRLQDAKDKAIQSINQALANKLKEIEASN
                     ATDQDKLIAKNKAEELANSIINNINKATSNQDVSQVQTAGNHAIEQVHANEIPKAKID
                     ANKDVDKQVQALIDEIDRNPILTDKEKQALKDRINQILQQGHNDINNAMTKEEIEQAK
                     AQLGQALQDIKDLVKVKESAKQDIDKQVQALIDEIDRNPILTDKEKQALKDRINQILQ
                     QGHNGINNAMTKEEIEQAKAQLGQALQDIKDLVKAKESAKQDIDKQVQALIDEIDRNP
                     NLTDKEKQALKDRINQILQQGHNGINNAMTKEEIEHAKAQLAQALQDIKDLVKAKEDA
                     KNAIKALANAKRDQINSNPDLTLEQKAKALKEIDEAEKRALENIENAQTKDQLNQGLN
                     LGLDDIRNTHVWEVDAQPAVNEIFDATPEQILVNGELIVHRDDIITEQDILAHINLID
                     QLTAEIIDTPSTATISDSLTAKVEVTLLDGSKVIVNVPVKVVEKELTVVKQQAIESIE
                     NAAQQKINEINNHATLTPEQKEAAIAEVNKLKQQAIEQINNAADVHTVEEVQHQEQAH
                     IEQFNPDQFTIDQAKSNAIKSISDAIQHMIDEINASKDLTDKEKQEAISKLNQLKDQS
                     IQAIQRAQSIDEIAQQLEQFKAQLKAANPFAKELENRKKAAISKIKDISTDKIDRIRN
                     STIGTAEERQAAMNRINEIVLETIKDINNAQTPQQVEAALNNGIARILAVQIVTSDHS
                     KPSSNSDGQSNSHLHVGYGTVNHPFNSSPIGHKKKLDQDDEIDPLHMRHFGDRIGNVI
                     KNALGVVGISGLLASFWFFIAKRRRKEDEEEELEIRDNSKDKKKGSIEGTKHLPLLFA
                     KRRRKEDEEDAIVEEKDSLNNDESLDKVKHTPFFLPKRRRKEDEEDVEVTNENTDEKV
                     LQDNEHSPVLIAKRLKDKDGNVETTTSIESKDEDVPLLLAKKKNQKDNQSKGKKSASK
                     KPSKKVAAKKKKKKSKKIKNNLFLR"
     gene            complement(36046..37437)
                     /gene="norB"
                     /locus_tag="O0V33_000171"
     CDS             complement(36046..37437)
                     /gene="norB"
                     /locus_tag="O0V33_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HCX8974867.1"
                     /translation="MEKPSRETFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNI
                     LITKGSELGVTSLLFITILAIAIVSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTFGCKKP
                     MLIGTAVLIVGECLISLTFLPEILYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIDTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
     gene            complement(37594..38916)
                     /locus_tag="O0V33_000172"
     CDS             complement(37594..38916)
                     /locus_tag="O0V33_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCX8974868.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
     gene            complement(38947..39987)
                     /gene="tdcB"
                     /locus_tag="O0V33_000173"
     CDS             complement(38947..39987)
                     /gene="tdcB"
                     /locus_tag="O0V33_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCX8974869.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQS
                     ITKGNVFLKLENMQFTGSFKFRGASNKINHLTDEQKEKGIIAASAGNHAQGVALTAKL
                     LGIDATIVMPETAPQAKQQATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSEN
                     VHGMAESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAM
                     KDLMQRAKIITEGAGALPTAAILSGKINNKWLEDKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            complement(40082..41200)
                     /gene="ald"
                     /locus_tag="O0V33_000174"
     CDS             complement(40082..41200)
                     /gene="ald"
                     /locus_tag="O0V33_000174"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCX8974870.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLATSHDLDYKEILNVIE"
     gene            complement(41675..42553)
                     /locus_tag="O0V33_000175"
     CDS             complement(41675..42553)
                     /locus_tag="O0V33_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HCX8974871.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGVPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSIC
                     NEHELHVSGKYISSHF"
     gene            complement(42573..46013)
                     /locus_tag="O0V33_000176"
     CDS             complement(42573..46013)
                     /locus_tag="O0V33_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCX8974872.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFATFKSRVEKSIA
                     DWDIKLEHTFYVSKFDHSENELGALSTYLVSLDQHRETIEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGLFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEGLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLVDRQETTYQVATDNAKDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDISFSTIEDFINSDLEKLKSHLNKNQ
                     LAFVHAVEKHYNLYVSMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQAFKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLQQMDHSYVEMITEFETNKADISRRQHRLTVYK
                     DKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDIKSVFNSQMTQNSDFNEEK
                     KVSTKVYLDQIHQRLFLEQSLITERIKKYFKNQLTEQIAPIVQQLADLHVIVNPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLGLLQPQMGLL
                     RQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY
                     "
     gene            46317..47658
                     /locus_tag="O0V33_000177"
                     /pseudo
     CDS             46317..47658
                     /locus_tag="O0V33_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
     gene            complement(48003..48335)
                     /locus_tag="O0V33_000178"
     CDS             complement(48003..48335)
                     /locus_tag="O0V33_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCX8974873.1"
                     /translation="MGKKMGLGLSIALVVIGIAVVCLMIFSSQKTTYFGYMNSNTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMM
                     KIHDMHMN"
     gene            complement(48419..49564)
                     /locus_tag="O0V33_000179"
     CDS             complement(48419..49564)
                     /locus_tag="O0V33_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCX8974874.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMQANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILTSNKEFEYLVDRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN"
     gene            complement(49773..50152)
                     /gene="rnpB"
                     /locus_tag="O0V33_000180"
     ncRNA           complement(49773..50152)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="O0V33_000180"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(50215..50559)
                     /gene="gpsB"
                     /locus_tag="O0V33_000181"
     CDS             complement(50215..50559)
                     /gene="gpsB"
                     /locus_tag="O0V33_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCX8974875.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILK
                     RISNLEKAVFGK"
     gene            complement(50573..51136)
                     /locus_tag="O0V33_000182"
     CDS             complement(50573..51136)
                     /locus_tag="O0V33_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCX8974876.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            complement(51129..51479)
                     /locus_tag="O0V33_000183"
     CDS             complement(51129..51479)
                     /locus_tag="O0V33_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HCX8974877.1"
                     /translation="MNDVVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     LLNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTYG"
BASE COUNT        13566 a         9245 c         8644 g        20236 t
ORIGIN      
        1 aatgacagac accattttta atattataaa atgtgttaac ctttatattt atttatgtgt
       61 actatttaca attttcgtca aaggcatcct ttaagtccat tgcaatgtca ttaatatctc
      121 taccttcgat aaattctcta ggcataaaat aaactaaatc tcgacctttg aataaagcat
      181 acgaaggact agatggtacc tgctgaatga attctcgcat tgtagcagtt gcttctttat
      241 cttgcccagc aaaaactgta actgtatttg taggtctatg ttcattttgt gttgcaacag
      301 ctactgcagc tggtcttgct aatccagctg cacagccgca tgtagagtta ataactacaa
      361 aagtagtgtc atcagcattt acttggttca tatactctga tacttcctcg ctcgtttcta
      421 aacttgtaaa accattttga gttaattcgc cacgcatttg ttgcgcaatt tctttcatat
      481 aagcatcata tgcattcata tttaattcct ccaattaaat tgttctgttc gtcatttgtt
      541 tccatactga accaagtgct tcatctccgt tttcaatacg agatatggcc atttcaattt
      601 gtaatttaac ttcaaacgca ttgtcattaa tatgggcttt aagtgcggga agctgctctg
      661 cattaccttc atcaaagata aacatagcag cacgccacct aacgattttc tgtggatcat
      721 ctaatagtag caccatttct ggtagtgcct ctggataccc taaatcgctt atgcaatccc
      781 ctgcagttct tcttacagca ggacttttat cacgaagccc cttatataaa tacggtaaaa
      841 tttctttact ttcaatcata cctaatagta caatcgcttg acgtctaacc ggtacttttt
      901 catcagataa agctaaatca agcagcggta tatcttcaaa agtcggcttt ggaaaatgat
      961 ttaacattct taatcgagtc ttccaattat cagtcgcatg atattcatcc aaagagacat
     1021 gtcgatagaa atgataatta ttagttgcgt gattttcttc taaagcatgt tttaccaata
     1081 caggtaactg tgattctgga taggtagcta gtacttcttc taggacacca tccattactt
     1141 cttcaatatt tccatagcga tttcctaaat ctagccattt acgcataaaa acaatattgt
     1201 catgttttgt ttgcgcttga gtcatatgat caacatatgt ttgtggtaat tgttctctta
     1261 attcttggtc agcagaagtt agcttaattt gatacggtat acccttaaaa gttaatagtt
     1321 cagctttaat ttcaccaaaa tgattgtcaa tttgtgggtc ttttccagat tccaacacct
     1381 gactttcacc tgaaaaagca gctttaatat caggtagtat gtcttcccaa tcagcttttg
     1441 gtgccttatc aacagctaag aagttcatga catgaaaaat ggaagtgata ccatctatag
     1501 ataacaactg atttataaat cttggttgat tttcttctac ttttttataa gtattagata
     1561 acttgtcttc tcttgtatat gacaaaacaa ctttcattgt atttggacta ggtgttggct
     1621 ctatacgtaa aatttccata tactttattc cccccgaata ctagttgatg aaaaatttat
     1681 tcgaatacag cctctacttt tggcaatact gtttcccaat ttgcgtcatt ttctttatct
     1741 actgaaataa agtccataac atggaaaatt gatttaacgc cttcaacctt taagatgtca
     1801 ttaataaatg ctggctgtga atcatcaact ttagtatacg tatctgatgt cataccttct
     1861 ctgctttcac taagtgtaat cttcattgtg ttgtggttcg gtgtttctga tatagatata
     1921 atttgcattt gacttcctcc tattgaagtt catttcctaa ttgttttatt tctttcccta
     1981 ttgaacactc attgatacaa aaatgatgcg ccttcgtttt cccctctaat ttacgcagac
     2041 gagttttgat tggacattga ttgcaataag tattcattaa atcatctatt ttcgcgagtg
     2101 cattttgctc tgaataagtt aaaatacgcg acacttcctt tattacttca aaatattgca
     2161 accttgtaaa taagtgttac tgttttaaat ttgacataaa aatattataa catttttgac
     2221 cctaagattt gaatgaaacc ttttacaatt ctatacgtaa aatgtattta tattcaaatg
     2281 ctattgcatc gcaaacgatt tttcagtaaa atgagtaagt tatttattga gcggtttcta
     2341 aacacccgta tgatcaaaat ttaggaggac attttaatat gtataatgaa attttaggac
     2401 tagttacgtt tattgcaacg ttcgtgctta tggtacttat gtatcgcttt tttggtaaac
     2461 aaggtttaat tgcatgggtt gcaattggca caatcattgc caacatacaa gtgattaaaa
     2521 ctgtagaaat ctttggtatt tcagcaactt taggtaatgt catgtttgct tctatttatt
     2581 tagcaacaga tatattaaac gatatttatg ggcgtagagt tgcaaaaaga gcagtttggt
     2641 taggcttttc atcaacatta attatgatta ttgttatgca attgtcatta cattttattc
     2701 ctgcaccaga agatatggca caaaaagcat tacacgcaat ctttgatgtt gtgccacgta
     2761 tagctttagg ctcaattgtc gcatatatta ttggtcaaca tattgatgta tttatctttt
     2821 cactaattaa aaaagtattt agttctgata aaacgttttt catccgtgca tacggtagta
     2881 catttttaag ctcaataatt gatacagcta tatttgtagc tatcgcattt atcggaagtt
     2941 tacctggtac agttgtattt gaaatattta ttacaacgta cgtactaaaa ttagcttcaa
     3001 cagttttcaa tgtaccattt ggatatattg ctaaatcatt ttatcgtaaa ggtaagattg
     3061 ataaattaga tcaaggctat taaagcactt actacataaa agaataagtt ttaaggctga
     3121 tatttgagaa aattttatca agatgatttt tttaggaagt atattacagt actacgtatt
     3181 agaaaatcat ctttgactca gcgagtattt taaacacgtg ttacttattt aagtgatgcg
     3241 tgttttttta tggattaccc aacgtatttt gtattacagt aatagtaact ttaactaagt
     3301 attgaataga aagtaggttt attatggcga aaataaattt tgatgctgcg acgaaaggaa
     3361 atccaggcat aagtacttgt gccattgtaa tcaaagaaga tgagcagcat tatacatata
     3421 cacatgagtt aggtgaaatg gataatcaca cagcagaatg ggcagcatgt atttatgcac
     3481 tagaacatgc acgtgaatta aatgttagta atgcgttgtt gtacactgac tctaagttaa
     3541 ttgcggatag tgttaacgct ggttatgtaa aaaatgccaa attcaaacct tattttgatc
     3601 aattggaaat atttgaacaa gattttgatt tattatttgt taaatggatt ccgagagaac
     3661 aaaacaaaga agcgaatcaa catgcacagc aagcattgta caaatttatt aaaaagaaca
     3721 aataaaagga gcaccgactg atatattttc aatcggtgct cctcttttta tcgaagaaac
     3781 aaattatttt ttattttttt agatttcttt ttcttctttt tagctgctac ctttttagaa
     3841 ggtttttttg atgccgactt tttccctttg gattggttat ctttttgatt tttcttttta
     3901 gccaataata aaggaacgtc ttcatcttta gattcaatac tagttgttgt ttcaacattt
     3961 ccatctttat ctttgagtcg ttttgcgatt aagaccggtg aatgttcgtt atcttgcaac
     4021 actttttcat ctgtgttttc atttgtaact tccacatctt cttcatcttc ttttctgcga
     4081 cgtttaggta agaagaacgg cgtatgttta actttatcga gtgactcgtc attgtttagc
     4141 gaatcttttt cttcaacaat agcatcttct tcatcttctt ttctacgacg tttcgcaaat
     4201 aaaagtggta aatgtttagt accttctatt gaacctttct tcttgtcttt gctattatct
     4261 ctaatttcca attcttcctc ttcatcctct ttacgacgac gtttcgcaat gaagaaccag
     4321 aagctagcaa gtaatccaga aataccaaca acgccaagtg cgtttttgat aacattacca
     4381 attcggtcac cgaaatgacg catatgaagt ggatcgattt catcatcttg atccaatttc
     4441 ttcttatgtc caataggcga actattgaat ggatgattta cagtgccata accaacgtgt
     4501 aaatgactat tagattgacc atctgaattt gatgaaggtt tgctatgatc tgatgttaca
     4561 atttgtactg ctaaaattcg tgcgatacca ttattcaatg cagcctcaac ttgttgtggc
     4621 gtttgcgcat tattaatatc tttgattgtt tcaagcacaa tttcgttaat tcgattcatt
     4681 gctgcttgtc tttcttcagc agttccaatt gtgctatttc taatgcgatc aattttatct
     4741 gtagaaatat ctttaatttt tgaaatcgca gcttttttac ggttttccaa ttctttagca
     4801 aatggattgg ccgcttttag ttgcgcttta aattgctcta attgttgcgc aatttcatcg
     4861 atgctttgcg cacgttgaat cgcctgaatt gattgatctt ttaactgatt taatttagat
     4921 atagcttctt gcttctcttt atcagttaaa tccttactag cattaatttc atcaatcata
     4981 tgttggatag catcagaaat tgatttaatg gcatttgatt tggcttggtc aattgtaaat
     5041 tgatctggat tgaattgttc aatatgcgcc tgttcttggt gttgaacttc ttccaccgta
     5101 tgaacatctg ctgcgttgtt aatttgctca atagcttgtt gtttaagctt attaacttcc
     5161 gcaatcgcag cttctttttg ttcaggtgtt aatgttgcgt gattattgat ttcattaatc
     5221 ttttgttgtg ccgcattttc aattgattca atcgcctgct gtttaactac tgtcaattct
     5281 ttttctacta ctttcacagg aacattaaca atcacttttg atccatcaag caatgttact
     5341 tcaacttttg ctgttaaact atcagaaatt gttgcagttg acggtgtatc aataatttcc
     5401 gctgtaagtt gatcaattaa gttgatgtgt gcaagaatat cttgttctgt aatgatgtca
     5461 tcacgatgga caatgagttc gccatttaca aggatttgtt caggagttgc gtcaaaaatt
     5521 tcatttacag caggttgtgc atcaacttcc catacatgtg tatttctaat atcatctaat
     5581 cctaaattta atccttgatt taattgatct ttagtttgag cattttcaat gttttctagt
     5641 gctcgttttt cagcttcgtc aatttctttg agcgcttttg ctttttgttc aagtgttaaa
     5701 tcagggtttg aattgatttg atcacgcttc gcatttgcta atgctttaat tgcatttttc
     5761 gcatcttctt tagctttcac taaatcttta atgtcttgta atgcttgtgc aagctgcgct
     5821 ttggcatgct caatttcttc tttagtcatc gcattgttaa tgccgttatg accttgttgc
     5881 aagatttgat ttattcgatc tttaagcgct tgtttttcct tatctgttag atttggattt
     5941 cgatcaattt cgtcaattaa tgcttgaact tgcttatcaa tatcttgttt cgcgctttct
     6001 ttagctttca ctaaatcttt aatgtcttgt aacgcttgtc caagttgtgc tttggcttgt
     6061 tcaatttctt ctttagtcat cgcattgtta atgccgttat gaccttgttg caggatttga
     6121 tttattcgat ctttaagcgc ttgtttttcc ttatctgtta gaattggatt tcgatcaatt
     6181 tcgtcaatta atgcttgaac ttgcttatca atatcttgtt tcgcgctttc tttaactttc
     6241 actaaatctt taatgtcttg taacgcttgt ccaagttgtg ctttggcttg ttcaatttct
     6301 tctttagtca tcgcattgtt aatgtcgtta tgaccttgtt gaagtatttg gttaattcga
     6361 tctttaagcg cttgtttttc cttatctgtt agaattggat ttcgatcaat ttcgtcaatt
     6421 aatgcttgaa cttgcttgtc aacatcttta ttcgcatcaa ttttagcttt cggtatttca
     6481 ttggcatgca cttgttcaat cgcatggttg ccagctgttt gaacttgaga tacatcctga
     6541 ttacttgttg ctttattaat gttgttgatg atgctgtttg ccaattcttc tgctttattt
     6601 ttcgcaataa gcttgtcttg atccgtcgca tttgaagctt cgatttcttt tagcttatta
     6661 gctaaagctt gattaataga ttgaattgcc ttgtctttag catcttgtag tcgttgatca
     6721 ccattaagat tttggattgc atcattgact gtttgaattg cgccgttaat atcattcaca
     6781 tttgtgttat cactatttag caatgtattt gctagacgtt tagcatcatt gaagtttgtt
     6841 ttagcattat cttcagcgtt ttggtaattg acagtttgct ctgcatttgg aatttcattg
     6901 tcaactaaat gtttcaatgt ttccattgca tcatttaagt caatttgatt attaacaata
     6961 tctgttacat ctgatacagt atcggcattg ttaattgcat tatgtgcaag atgttgttgc
     7021 tgttgattta atccatttaa cgaattaaca aacgcatttg ctttatcttt ggcatttgca
     7081 aggttttggt ctccatttaa tgcattttga gcatcaataa tattttgttt caattgctct
     7141 gcttcagctt ttgcaattgc attaccttgc actttatcta actcatgtgc tgcattcgca
     7201 attgcattat cataatttgc tttcaaatta tcatctgcat tgatgtaatt tgtgctgctg
     7261 ataacaccaa ggtgctgttc gtcaatatat tgttgtactg cacccattgc attatctaat
     7321 ttagttgctc tattaattgc tcgagatgct ttatttagcg tttcagcatt attaatttgt
     7381 tggattgcta attgtctttg agcattgttt agatgtgtta attgatccaa tctttgtaat
     7441 gcttcagact tacgattatt aagtttttct tcaccattta atgctttctt tgctgcagtg
     7501 actgcatcat ttaatttgat aacttgttct gcagttaagt tttgacctgt cgatttatca
     7561 agtgcttgtt tcgctttatc aaccgcttca tcatacactt gtttaacttg tgtatctgca
     7621 ttgacatagt tcgtgctacc tttcgtgcgt ccttcattgc cagtgattcc ttgtgacaat
     7681 tgatccatta cacgattaag tgccttagcc tcatctacaa tttgttggat ttgatttaaa
     7741 tcgtttgatt gatcgatgcg acctttaaat ccatcttgtt gagcattgtt taatgcattc
     7801 aattgctcta tatcatgttt accatttgtt ttagcaagtg ctacacgttc atcaccattt
     7861 aatgcttgtt ttgcatttaa aatattttga agtgcttgat ccacttgttg cttattcgca
     7921 ttttgtttca atacattttc ggcatcagca atagcttgtt tataagcatt ttgcttatct
     7981 aaatctgctt gtgtgtaatc tacagtttgc tcaacgttag catgttcatt aactgcttgt
     8041 tgtaattgat ccattgattg gttcaattgc gttgcttgat ctactaattc agcgattggt
     8101 tgaagtttcg tcgcttgatc aatattttgt ttagcagttg caatttgatc agcatttaaa
     8161 tgtgttaatt ggtcaatagt ttgtttcgct tgtgttttag cttcagcgac tctctcatta
     8221 ccatttaact cattcacttt ttcttgaagc ttagttaatg cttggtctac agcgtcttta
     8281 ttcgcatttg aaccattagt tggatctgta atgctttctg cagcttgtaa cgcttgatct
     8341 actgcttctt ttttatcagt tgacgcttca gtgtaatttg gttgagcctt atctgtattc
     8401 acttgatcaa ctttattttt caatgtttcc atcgcgtgat caagttcagt agcagtttga
     8461 acatgttgtg caacctctgt tcttgtaggc gctgcattta tagcatcagt taatgcttgt
     8521 tgttgtgcat gatttaagtt tggcaatgca tttacagttg ttactgcttg tagttgatca
     8581 cgagcaagtt tttgatcacc atgtaggtta tcttttgcag ttgttactgc ttgtgtcaat
     8641 tgttctactt gtgatttgtc gagtgttgga ttacctgttt ggttaattaa atcttttgca
     8701 ttttgaactg ctgcttggta agcatctttt tgcggtttat cttcattgat aaacttgcta
     8761 ccagattctg tttgttgttg atcttgaata ctattacgta atgcttgcat tgcttgatca
     8821 agcgcttttg cttcagctaa tttttgcgct acttcatcac gagttgctgc gttgtttatt
     8881 tggctttcaa gtgcttgacg ttgcggatta ttcaaaccat ttaattgatt taaatcagta
     8941 accgcatgtt gtttatcgtc tgcaagtttt tgatcaccgt gtaggttatc tttagcttgg
     9001 ttaacagctt gtgtcaattg ttcaacttgt gctttatcaa gcgttggatt gttagtttga
     9061 ttaattaaat cttttgcatt ttgaactgct gcttggtaag catctttttg tggtttatct
     9121 tcattgataa acttgctacc cgcttccgtt tgctgttgat cttgaatgct attacgtaaa
     9181 gcttccattg cttggttaag tgcttctgct tcagttaatt tttgtgctac ttcgccacga
     9241 gttgctgcat tattgatttg attttcaagt gcttgacgtt gtggtgtatt caagttagac
     9301 aagttattta acgtttctgt tgcacgttgc ttatcttgat ctagtttttg atcaccatgt
     9361 aaattgtttt tagcatcagt cactgcctgt gtcgcttgat caatgattga tttagctaat
     9421 gtaggatcag ttgttttgtt aatcaaatct ttcgcgtgtt gtacagcttg cgtataagca
     9481 tctttttgtg cttgatcttc attaataaat ttactacttg cttcagtttg tggttgatct
     9541 ttaatacttt cttttaatgc tttcatcgct tcatttaatg cttgcgcttg tgcaataatt
     9601 tcagccactt tatcacgagt tgttgcatta ttaatttgat tttctagtgc ttgttgttgt
     9661 gctggtgtta attggtctaa atgatttaga gaatttccag cagtttgctt atcttgcgct
     9721 aatcgttcaa caccgtctaa tgcattttta gatgatgtta cagcttgtgt cgcacttgtt
     9781 acattacctt tattgatagt cggattattt aatcccgcaa tgatagactc agcattttga
     9841 actgcttcat catatgattg ttttttattc ggttctgcat tgacatatgc actgctcgca
     9901 cgtgttgcct ctttgtcagc aatactttgt tgtaatgcat ccataagttg atctaatgct
     9961 tgtgcttcag tcaaatcatg gttaacggct gttctagttg tttcactatc aatcaacgta
    10021 tcttccatat gtttttgagc attgtttaaa tgtgttaatt gactaactgt ttgtttcgca
    10081 tgatctttat cattttgtaa tttcacatca ccatgtaatg ctgctttcgt tgtattcaca
    10141 gttgtcgctg cttgattaat tgcattatta tctaatgttg ctgtttgttc attgataata
    10201 ttatttgcgg cttgcactgc ttgatcgtag ttttgttgtt ctgattgatc ttcgttgata
    10261 tatttgctat ttgctttagt tgctgcgtta tcttgaatac tttctcttaa gccatgcatt
    10321 gcttggttaa gcgtattcgc attttgttca atttgatgaa ctgcagttac taaagttgca
    10381 gcagtaactt ggtcttttaa tgctgttttt tgaggatcat ttaagcttgt taaagcatca
    10441 attgcttgcg tcgcatttgc tttagcaact tgtaaattat gatctccatt taacgcttga
    10501 agtgttgttt gaactttagt aatagcttgt tctacttctg tttggttcgc attttgacca
    10561 gtttgtttgt taatcatggc ttctgcttca tttaccgctg tagtataagc agtttgttta
    10621 tcagtatcag cattgacgaa gttaccaagt tgttcaacgg tttgatgatc tgcaatcgca
    10681 ttaattaagt tacccattgc tgtatttaaa gattctgctg tttgtttgtg accattaact
    10741 gcagcaatat cagatgattg cgtaatttgt gattgtaact gttgcttttg cgcattattt
    10801 aaatgatcta attgatcaag tctattgttc gcttgttgct tagctgctac aaggttttca
    10861 tcaccatcta atgcaacttt cgtgttattc acttgtgtcg ttgcacgatt aacttcatct
    10921 ggattcattg taggtgatgt tgtttggtta atgatattat tagtagctgt tactgcttgg
    10981 ttatagtcat tttgtctttg cgcagttgca tcatgataat cttcagacgc taatgtcgct
    11041 tgattatccg caacactatt tcttaataat tccatcgcat gatctaacgc ttgtgctttt
    11101 tgattaattg tagtgacatc tgtaacaagt ggcgcttgtg taatttgact ttcaaatgat
    11161 tgtttttgtg catcatttaa gtgtgttaga ttcgccagtt gttgtttcgc ttgttgctta
    11221 gcattcgcta aattatcatt accatttaat gcttgtttcg ctgcgttcac ttcattagtg
    11281 atgctttcaa cttgtgcttg tacttcatta gtaccacttt gtttattcaa taatgcttca
    11341 gcttttgcta ctgcctgttt atacgcattt tgtttgtcag gatctgcatt taagtaatta
    11401 ccgtccgcta atgtttgagc tttatcatta atagcttgtg ataattgatc cattgcatga
    11461 tctaggctcg ttgctgtttg tttaacttgg ttcactatag caatttcagg cgattgatcg
    11521 atttgtcctt ttaatgcttg tttttgagca tcgttcaaat gcgtcattgc attgacagca
    11581 tctttagcat tttgcttttc agttgccaat ttagcttcac cgtttaagcc atttttagca
    11641 tcagtcactt gagttaatgc acgagtaata tcatctgggt taagcattgg atttgtcgtt
    11701 tgattgataa taccttccgc ttgagacact gcatttgtat atgctgtttg cttatcggca
    11761 tcagcatcgt gatagttttc acttgctttg acggtatctt tatttgcaat accttgtttc
    11821 aagttactca ttgctgtatt aacattttgt gcattttgtt tagtttgttc aactgtagct
    11881 aacgcttgtg cttgattgat ttgattacgt aacgcatcac gttgtggttg atttaagtca
    11941 cgcaacgtat ctagatttgc tattgcatct tgtttcgctt gcgctaattt ttcatcaccg
    12001 tttaatgcat ctttcgcttg attcattgta gtaactgctt gtgttactgc gtcaggcgtc
    12061 aatacaggtg ttggtgtgcc atttgcaatt tgttgcgctt ggttagctgc attgttatat
    12121 gcttgttgtt tgtcttgatc cgcttgtgta aagtcaactg attgtggtac ttgactgttt
    12181 gcttgaattt gttgtttcaa cgtacccatt gcattattta atgcatcagc attttgctta
    12241 atagcattaa cacctgtaac aagttctgca tgtgacactt ggtcttttaa tgctgttttt
    12301 tgtgtttgat ttaagtttgg taactgatca atggcagtat tcgcattgtc tttagcaact
    12361 tgtaagttat ggttaccgtt taaatcaccc ttagcttgag tcacttgttg taacgcttga
    12421 gccacttgtt gtggatccac gtttgcattt ggtgtaccac taatgatttg ttcagcatgt
    12481 gctaccgcat tgttgtatgc attttgctta tcagtatcag cattgacaaa gttaccattt
    12541 gctttagttt ggtctttatc tgcaatacct tgtttcaatt gtgtcattgc ttgatttaaa
    12601 tcttgtgctg tttgcttaat cgtgtttaca tttgcaacag tttgcgcagc atcgacttgt
    12661 tgttttaacg catctttttg cgcttgattt aaatcattcg catttgtaat cacttgtttc
    12721 gctgcatctt ttgcacgttg tacattgtca ttaccattca atgcttgttt cgcttcgttc
    12781 acgcgttgca tcgcttgttc cacttcggtt tgacttgcat tgctgccatt tgctttagat
    12841 aaaatacctt ctgcatgtgc aaccgcatta gtataagcat ctttcttagc ttgatctgca
    12901 tcagtaaagt taatgctatt taatgtctca gttttatctt gtaatgcatt acttaagtta
    12961 gtcatagcat tattcagatt ttgagcattt tgcttaacat tattaactgt tgcaatatct
    13021 ggtgcttgtt caacttgtgt tgttaacgct tgtttttgcg catcatttaa atgatctaat
    13081 gtgccaagat ttgtcttagc agcttgttta gcatcagcta atttttgatt accgttaagt
    13141 gcttgatttg tatcattcac tttagttaat gcttgttgaa ttgtatttgg atccattgtt
    13201 ggatttgttg tttgttttaa caattcttct gcttttgtta ctgcattatc atatgctgtt
    13261 tgcttgtcag tatcagcatc gtgatagttc tcgctacctt taatttgtgc tttattcgca
    13321 ataccttgtt tcaattgtgt catcacatca ttaagcgtag ttgccgcttg ttgaatagca
    13381 ttgacgtttg gtacaagtgt tgcttgcgta atttgtttgc tgtattcatc acgttgcgct
    13441 tgattcaagt ttggcaattg atcaatagca tgttgaacat tttgtttcgt tgttgctaag
    13501 ttcgtattac catttaaatc atttttagct tgtgtaactt tatttaacgc tgcagtaatt
    13561 tcgctaggtg taacgacaac atcaggtgtg ccgctaatta atgcttcagc ttttgctact
    13621 gcttgtttat atgcattttg tttgtcagga tctgcattga caaagttacc atcagctttt
    13681 gtttgttctt tatctgcaat gccttgtttt aattgtgtca ttgcattgtt taactcttgc
    13741 gctgtttgtt taacattgtt tacaccagct acagtagttg cattttgaac ttgctgtttt
    13801 aatgcgtctt tttgtgcttg gttaaggtca ttagagctat taattaatgc tgttgcttcg
    13861 tctttcgcat gttgaacgtt ggcattacca tttaatgctt gttttgcagc attaacttgt
    13921 tggattgctt gttcaacttc agtttgtgtt gcattgccac cattagcttt tgaaatgatg
    13981 ttttcagcat ttgtaacagc agtgttatat gcttgttgtt ttgcttgatc tgcatcagtg
    14041 aagtttactg ttgctttcgt agcatctttg tcagcaatcg cttgtttcaa gttacccatc
    14101 gctgtattta agttttgcgc attttgttta atttgatttg cttcatcaac agtatgcgcg
    14161 ccattaattt gatcagtaac tgcttgtttt tgcgcattat ttaagttgtc taatgaacct
    14221 aatgattgtg tcgcttgttg tttcgctgcc tctaggtttt cattaccatt taatgcattt
    14281 ttagttgtgc ttacttgtcc agcagcttga ttgataacag tcggatctaa agttggactt
    14341 gtcgtttgat caataatacc ttgtgcagtt gtgacagcat tattgtacgc atccttttta
    14401 ttcggacttg aatcagtata attttggttt tgttttgttg tcgcattatc tgcaatactt
    14461 tgacgtaatt tgtccattgc tgcatcaaca ttgtttgctt tttgttcatt accttgtgct
    14521 tctgcaacag tagtcgattg ttgtaccaat tgttttaatg cctctttttg tgcatttgtt
    14581 aaatggctta aaccgtcaat cgctgtattt gcgtgttgtt tagctttttc aaggttttga
    14641 gtaccattaa gcgctgcttt tgtagtattc acttgattca tagctgcttc aacttgatct
    14701 ttagacacgt ttgtacctgt agatttattt aaaatatttt cagcattacg aactgcttca
    14761 ttgtatgcat ttttcttctc tggatctgca tctgcaaagt tttgactgcc aagtgtagtg
    14821 tctttatcat tcaagctatt tttcaagtta cccatcgctg tatttagttc ctgagctgtt
    14881 tgaatggctt gcgttacttc agcaacagtg tgcgcttgat tgatgtttcc agtaacagca
    14941 cctttttgtg cattgtttaa gttatctaac gtagataatg tttggtttgc attgttttta
    15001 gcatctgtta cttttctatc accattcaat gcttgtttag tagttgttac agttgaaagt
    15061 gctgcttcaa cctgtgattg gttcgcattt gtaccatttg cttgattaat aatattttca
    15121 gcagcagtta ccgcattgtt gtatgctgtt tgtttttctg gatctgcatc tgtaaagtta
    15181 acactttgtt tcacttgatc tttgtcttgg attccgttga ttaaacgttc cattgcttga
    15241 tctaattgtt cagctttagt tttcacttga cttactgtgc taatatttgg tgcatttgtc
    15301 acttgcgtat ttaaatcttg cttttgagca ttatttaaat gacttaattg gttgatgttg
    15361 gcagttgttt cttgcttttt atttgctaag ttttgtgcac cattaagcgc agttttgtta
    15421 gtgttcactt gattagctgc ttgtgttact gcagatggat caagtgtcgg ctgactcgtt
    15481 gcattaatga tattttcagc acttgtaaca gctgtgttgt atgcattttg tttatctcta
    15541 tctgcatcaa catatggttg actcgctttt acttcatctt tattcgcaat actatttcga
    15601 agttgattca ttgcattatc aagatttgtc gcgttgtctt tcacactttg aacgccagtt
    15661 acttgtgtcg cattatcaat ttgaacagta atactttgtt tttgtgcatc agataaatgt
    15721 gtcatttgac gtaatgcatc tttagcctgt tgcttcgcag ttgctaagtt tgtatcaccg
    15781 tttaaacctg tttcagcatt attgacacgt tgtgttgctg ctgtaacatc tgctgcattt
    15841 gtaatcacat taggtgaacc atttacaatg tttttcgcag cattatatgc atcagtataa
    15901 gcaccttgct tatcaggact cgcgttagtg taattaccac cagctacaat tgtgtcatga
    15961 tcagcaattg cttgttttaa ttgatccatc gcattattaa gtgatgtagc actatttttc
    16021 agatcagtta cacctgatac acgttgcgca gcattcactt gttgttttaa tgctgttttt
    16081 tgtggtgtat tcaaatgtgt caagttatcg atagcttgcg tagcttcagt tttagcacgt
    16141 tgtaagtttg cgtcaccgtt caaagcattt tcagtagacg ttacactttg aattgcttgt
    16201 tcaacagctt gcttatcttt attcgcaccg tgatctttat ttaagtatga ttctgcttgt
    16261 gtgatggcat tatcaaatgc tgttttgtta gttggatctg cgtcgatgta attttcgctc
    16321 gctttagttt gatctttatt tgcgattgct gattgtaatc gttgcattgc accatctaag
    16381 tcttgtgctt tcgttttaac accatttaca ccgttgactg ttgttgcgcc ttcgatttgt
    16441 gatgttaaat cttgtttttg tgcattattt aagtgtgtta atgtgcctaa gtttgctttc
    16501 gcatctgatt ttgcttgtct tacatttgcg tcaccgttta aagcattctt agcagtcgtt
    16561 acttgattta atgcagcttc aacttgtgct ttcgtcatat tttgaccgtg tgctttatct
    16621 aaaatacctt gagcattcgt aacagcattt gtgtaagcat ctttcttacc ttggtcagca
    16681 tctgtaaagt tcacttgttg tttaacagtg ttttggtcat taataccatt ttgaagttga
    16741 cccattgctg tatttaactc attagcagta ttttgtgcag cagttacgcc tgctactgtc
    16801 gttgcattgt caatttgact agttgctgca gttgattgcg cattattcaa gttactatat
    16861 gaagctaatg cacgtttcgc agtttcttta gcttgagcaa ctttattatc accattcaag
    16921 tcatttttag cttgattcaa ttggttaagc gctgcttgaa cttgtgtttc attcgcgttg
    16981 ctgccattag cattagtaat tgcttctgct tgtgtaaccg cagtatcata cgcttgttgt
    17041 ttaggttgat ccgcatctgt gtaattgaca ctacgtttcg tattatcctt ttcagcaagt
    17101 gcatgtttca aattacccat cgctgtattt aacgatgttg ctttattttt agccgctgtg
    17161 acagctgcaa gatttggtgc ttgatccact tcagcatttg ccgcagttcg ttgtgcattg
    17221 ttcaagtctg ttaaacgacc gatgtctgat ttcgcagttt gttttgctgc tgctaatttt
    17281 tcatcaccgt tcaatgcaga cttcgcactg ttcacttggc tcgctttttg attaatcgta
    17341 tctggattca tttcagggtt attcgttgca ctaataatac cttcagcatt agttaccgca
    17401 tcattgtatg cattttgtaa atctgtattc gcatcatgat aatcttcgct cgctttagtc
    17461 gcatctttag aagcaatgct ttgtctcaat tgattcattg cttcatttaa agtaccggca
    17521 ttagcttgaa taccttgaac acctgcaaca gtcgtaccgc gttcgatttg cgatgttaag
    17581 tttgcttttt gagcatcagt taaatgtgac attgtcgcta attgttgttt agctgtgttc
    17641 ttcgcttgat ttaatcgcgc gtcaccattt aatgcagttt tagcagtatt cacacgttgt
    17701 aatgcttgtt caacggctgc tttattgtca tttgtgccag ctgttttatc taataacgtt
    17761 ttagctgctg ttacagcatc attgtatgca gtttgtttat cactatctgc atcagtatat
    17821 ttctgagctg cttttgtagc tgcttcatca ttaataccat tttgtaagtt gctcattgct
    17881 gtatttaatt cagatgccgt tgcagacact tgatttacac ctgcaactgt tgtcgcacta
    17941 tcaatttgtg atttcaacgc ttcgttttgt gcattattaa ttgatgttaa attatttaat
    18001 gcatttttcg cagttgtctt agcatttgca acattttgat taccgttcaa atcgttttta
    18061 gcacgttgta cattttgtaa cgcagattca acattgtctt ttgcagtatt tggaccttgt
    18121 actttattta aaatttgttc agcttgcgtc actgcatttg tatatgcatt acgtttcgct
    18181 tcatctgcat cagtgaagtt aacaccttgc ttaatcgtat tttgatcttg aataccattt
    18241 ttcaagttcg tcatagctgt atttaatgcg tccgcattat tttgcgcttg tgttacttca
    18301 ctaacatgcg ttgcaccttc gatttggcgt tttgctgcat ctttttgtgc attagttaag
    18361 tcacttaagc cattcaaatg ttgtttcgca tttatttgtg cgtttgttaa gttttcttga
    18421 ccgtttaacg cttgttgttt agctgtcact tggtctttcg cttgattgat ttcttgagca
    18481 ttcattgttg gacttgatgt ttgaccaatg attgcttttg ctgcagtgac tgcactatcg
    18541 tattcactac gattattagg acttgcatcc gtgtagtttt gttctgcttt aatcgtcgcc
    18601 tcattcgcaa tgctatctcg tagacctttc attgcagtgt ttaatgtttg tgcattatca
    18661 cgaacagttt gaacatttgg caacgttgtc gcttgaccca cttgttcttt aagttttgct
    18721 ttttgcggac catttaaaga agttaaacca tcaattgctg tgttggcatt ttgtttcgct
    18781 acttctaaat tatgattacc gtttaactgc gtcttcgcat tcgttacttg cgttgctgcg
    18841 tttgtaacat ctgatggtgt taacgttggt gttggtgtac cactaacgat attttctgca
    18901 gctgtcactt tttcatcata tgcttggcgt ttattcgcat cagcattgac atagttacca
    18961 ctagcttttg tgtcagcttt atcagcaatg gcacgtttta aagctgtcat cgcagtattt
    19021 aattcagtag cagtatgttc aacaccattt gctacagata cgcgtcctgc acttgttact
    19081 tgtgctttca atgcttcttt ttgttttgta tttaagtccg aagcatttgt aatcgctgtg
    19141 ttagcagcct gtttcgcacg ttctaagttt tgaataccgt ttaatgcagt attggcttgt
    19201 gtaacagctt gcattgctct ttcgacatct gctttaggtg tattaccgcc agatgtttta
    19261 tttaaaattg tagctgctgc atttactgct tgagaataag cagttcgttt cgcatcatca
    19321 gcatcttgat aattttgact ttgtaacgtc gtgtctttat cacgaattga tgtttctaat
    19381 tgacccatag caccatctaa ttgttgcgct ttggctttaa ctgtattaac accttcaaca
    19441 tttgttgctt gtgtaatttc attatctaac gctgtacgtt gtgcattatt aatgtgtgtt
    19501 aatgtaccta acgtttgttt cgcagcggct ttagcttcat ttaatttagc atcaccgttc
    19561 aacgctgtct tagtactatt cacattttgt aatgcttgtt caactgctgc tttgtctaca
    19621 ttttgaccac tagcttttgt taaaattgct ttcgctgcat ttactgcttg atcataagct
    19681 gatttcttac ttggctctgc atctaggtat ttctgagttt gttttgtttg tgtctcatca
    19741 ttgataccat tttgtaaact gtgcattgcg ttatttaatt cagttgcttt gacagtttct
    19801 tgatttacac cagctactgt tgttgcacca tcaatgctac gcgttaacgc atctttttgt
    19861 gcattgttaa ttgatgttaa gttattcaag ttgtttttcg cagttgtctt agcttgagct
    19921 aagttttgcg caccatttaa tgcttgttct ttagttgtca cttgcgatgt tgcttgcgta
    19981 atagtatttg gttccatcgt tgggttcgat gtttgattaa tgattgcttt tgctgcagta
    20041 actgcgctgt cgtactcgtt acgattattt ggacttgcgt cagtgtagtt ttgacctgct
    20101 ttaattgttg cttcattcgc aatactatct cttaagcctt tcatcgctgt attcaacgtt
    20161 tgagaactat ttttaacagt ttgaacgcca tctaatgtag ttgcactttg aacttgttct
    20221 tttaattttg ctttttgtgc attattcaat tgcgctaagc catcaattgt attgttggca
    20281 tgctctttcg caacacgtaa gttgttatca ccatttaata ctgttttagc tgcattcact
    20341 tgtgaagttg cactatttac gtcattgact gttaatgtcg cattcggtgt accattaatg
    20401 atattttcgg cgttagtcac ttttgaatca tacgcttgac gtttttcttg atcagcattg
    20461 acataatttc cgcttgcttt tgttctttct ttatcagcaa tagctgcttt aagtactgtc
    20521 atcgcgccgt tcaattcagt agctgtatgt tgaacgttat ttgcatcaga tacacgttgt
    20581 gcattgttaa cttgtgtttt taatgcatct ttttgttttt gatttaagtc atgtgcgtta
    20641 ttaattgtgt tcgtcgcatt tgttttagct tgagctaagt tttgtacacc atttaacgct
    20701 gcttttgttg tattgacttg atttgccatg ccattaatcg cattagcatc catatttgga
    20761 ttgttcgttg cattaataac accatttgca ttatttacag cagtattgta attatttttg
    20821 ttgatatcag atgcatcaag ataattttga cttgttttcg tcgtattatc attttggata
    20881 cttgtacgta atgcacccat ggcagtattt aatgtattac ctttatcttt aacaccattt
    20941 acaccagcta cattttgcgc ttgttcaact tgatgcttca agttgtcttt ttgtaattgt
    21001 gttaagtgtg gtaaaccatt aatcgtatta gttgctgaag ttttcgcatt tcttaagttg
    21061 tcagcaccat ttaaagccgc tttcgctctt gtaactgcat ttaatgcatt atcaacgtct
    21121 gctttagatg tgttaccacc agtttgttta tttaaaatgc cttcagccgc tgtgacagct
    21181 tgcgtatatg catttcgttt tgattcatcc gcatcaagat aattttgatt tcttaatgtc
    21241 gcatctttat cattgattga accttgcaag ctattcattg cgccgtctaa tgtattggca
    21301 tttgttttca cagtattaac gccatcaaca gtcgtcgctt gattgatttg gccttctaag
    21361 tcagtacgtt gtgcattcgt aatatggttt aaagtgccta agttttgttt agctgccgct
    21421 ttcgcttctg ccagtttagc atccccattc aatgcatctt tcgtacttgt tacttgttgt
    21481 aatgcacggt caactgctgc tttatctgaa tttgatccag tttgtttatt taaaattgct
    21541 ttcgcagcag ctacagcttg atcataagca gttttcttac tttgctctgc atcacgatat
    21601 ttttgagttt gttttgttgc attttcatca tcaataccat gttgtaattg acccatagct
    21661 gtattaagtt catttgcagt ttgttggata cttgttacat ttgcaacacg ttgcgcactt
    21721 gtaacttgtg cttttaacgc atctttttgc gctttattta agttagtagc accatcaatc
    21781 gcatttgttg ctgtttgttt cgcttgcgtt aaattatgtg taccatctaa tgcatttttc
    21841 gttgatgtca cttgtgtagc gatttggtta attgcattag catccatatt tggattgctt
    21901 gttgcattaa tgacaccatt agcactatca acagcattgt tatagtttgt tttgttactt
    21961 tctgtagcat caagatagtt ttggccattt ttcgtagctg cgttatcttg aatgctattt
    22021 cttaacgtac ccatagcacc atttaatgtg ttggcacttg atttaacagt atctacacct
    22081 gcaacatttt gtgcttgttg cacttgttgt ttcaatttat ctttttgagc agttgttaaa
    22141 tgttgaagtc cgtttatagt agtgtttgca tttgatttag cttgctctaa attatggtta
    22201 ccatttaggg cacctttcgc atttgtcact tgtgacaacg cgttttcaac ggcagctttg
    22261 tctaaatttg atccactatt tttatttaaa atattttctg cgttcgtaac ggcttgagta
    22321 taagcagttt tcttatctga atcagcatca tgatagtttt cgctcgctaa agtattcgtt
    22381 ttatcattaa taccattttg caagttagcc atcgctgtat tcaattgcgt acctgtatta
    22441 gataccgctt caacaccaga tactgttgtt gcttgatcaa ttttagttgt taagtcacgt
    22501 ttttgtgcat tattaattga agttaaatta tttaatgcat gtttagctgc attcttcgca
    22561 tttgtgacat tttgatcacc attcaatgca tttttagcgc tagtaacact ttgaatagcc
    22621 gcttctacat cttgtttagg cgtatttgca ccttgcgttt tgttcagaat tgtttctgct
    22681 ctgcttaccg catttgtata cgcatcacgt ttggctttat ctgcatcagt gaagttaaca
    22741 ccttgtttaa ctgtgttttg atcatggata gcttgttcca agttgcccat ttgcgtgttt
    22801 aactcagttg ctgcattttt agcagctgtt acctcactca catgacctgc acgatcaatt
    22861 tgtgatgaga ttgcagattt ttggttattt gttaagtgtg ataacgtatt taagttttgc
    22921 ttagcagtgt tttgtgcatt tcttaagttt tcagcaccgt ttaaaccatt tttagcatta
    22981 ttcacttgtg ttgtagcttg ggtaatcgca ctagcatcca tagtcggatt gttagtttga
    23041 ttaatgatac ctttcgcatt tgacacagcg ctattgtatg ttgattggtt attcggactt
    23101 gcgtctgtat agttttgact tgctttgact gttgtttcgt tagcaatact atctcttaag
    23161 cctttcattg cattgttcaa tgattgtcca tttgtttgaa cagattgtac gtcttctaat
    23221 ctattggctt gtcccacttg ttcttttaat ttagcttttt gaggtgtatt taattgcgtt
    23281 aatgcatcaa tagcagtatt ggcgttttgt tttgcaacac gtaatctttc gtcaccattt
    23341 aattcttgtt ttgcgctgtt tacttgatta gctgcagctg ttacttctga aggtgtcgta
    23401 acaaccgttg gcgtaccgct aataatatgt tcagcattgg taactttagt tgtgtaaaca
    23461 ttttgtttag tgctatcggc gttaacaaat ttactgcttg ctaacgtagt cgtcttatct
    23521 gcgatggcat gttgtaattg acccattgcc gtgttcagtt cagtcgcatt acgttgtaca
    23581 tcttgtgcat tagatacgcg ttgagcacca ttagcttgtg cttttaatgc atctttttga
    23641 tgttgattta aatcagatgc gccattgatt gctgtaatcg ctgcttgttt cgcattattt
    23701 aagttttgcg taccatttaa tgcatgtttc gcactattaa cattattaag tgcttgttcg
    23761 actgcagttt tcgctgtatt cggtccagtt tgtttattta aaattgtttc ggcatttgat
    23821 acagcttgat tgtaagcatt acgtttttgc tcatcagcat ctaggaagtt ttggctcgct
    23881 aatgttcctg acttatcgtt gatagccgtt tgtaagttac ccatagcacc atctaaacta
    23941 ttcgcacttt gcttaacaga ttcaacacca gctaagtttg tagcttgtga aatttgattt
    24001 gttaaatcat tgcgttgtgc tgtagtgata tgcgttaaag tacctagatg ttgtttcgct
    24061 gcgttttgag ctgcaattaa tttggcatca ccattcaatg catttttcgc agttttcaca
    24121 cgttgcaatg ctgcttcaac tgcattttgc gcagtgtttg gaccatgttg tttatttaaa
    24181 atcgctttag ctgcagtaat agcattatca tactcttgtt gtttatttgt gtcagcatca
    24241 cgatatttct cagatgattt cacttgagat tcgttgttaa taccattttg caagctagcc
    24301 atagcttggt ctagatgttg cgcattttgt tttacagtat caacactact cactcttgtc
    24361 gcactactaa tttgactaat caaattgttc ttttgagcat cagtaatact tgtcaatgtg
    24421 tttaagtatg tttttgcatt tagttttgcc gtagctaagt tttcatcacc gttaagtgcc
    24481 gttttagaac tattcacttg ctctgctttt tgtgtaatcg tacttggatt catttctgga
    24541 ttactattcg cattaataat cgtttcggca gcagctactg cgttgttata tgctgtttgc
    24601 ttatcattat tagcatctac gtaatcttca cttgctttag tcgcatcttt gttggcaata
    24661 ctttgtctta acgtattcat cgcttgatct aatgtattcg catttgattg agccgtatga
    24721 acaccagcga cagtagtacc actattaatt tgatttgtta aatgcgtttt ttgagcagtt
    24781 gttaaatgcg tcatattatt taactgctgt ttcgctgttt gctttgcttg atctaataaa
    24841 cgcgtaccat ctaagttatt tttagccgaa ttcacttgat tcatcgcttc tgtaacttga
    24901 tccttcgttt tattttgacc atttgatttg tttaaaatat ctttcgctgc ttgtaccgca
    24961 tttgtgtatg ctgttttctt acttggtgtc gcatcttgat aattttgact atttaatgtc
    25021 gtttgttcat cattgattgc accttgcaag ttacccatcg ctgtatttaa atctgtacct
    25081 tgttgtttaa cagtatttac accagcaata tttgatgcag cattaatttt agatttaaca
    25141 tctgcttttt gagcattatt taaatgtggt aatgcatcaa ttgcacttgc agcatctgtt
    25201 ttagattgtg ctaatttttc atcaccattt aatgcatttt tattagtagt aacagctgaa
    25261 gttgcactgt taacatcatt aacagacatc gttggatttg ttgtttgatt aatgattgtt
    25321 tcagcacttg aaacggcgct attatatgca ttttgtttag ctgtatctgc atccgcataa
    25381 tcttctgtac gtttcacttg ctctttatct gcaacagctt gtcttaagtt acccattgca
    25441 ctatttaagt tagttgcatt ttgcttaatc gtattaactg tctcgatttg atgcgcacca
    25501 ttaatttgcg attgtaagtt ttgacgttgt gcattattca agttatttaa ttgacctaat
    25561 gctgtgttcg cttcttgctt cgcagcattt aacttagctt caccattcaa tgcctgttct
    25621 ttacttgtga catttgataa agcattgtta acatcacttg gtgttataac tggatgttgt
    25681 gcattaccat taataatgtc attcgcatgg ttgtatgcat tgttgtaatc atttttctta
    25741 ttagtatctg cgttgacata attatcactt tgtacgactt ggttatgatt agcaacgcca
    25801 cgttttaaac ctgtcatagc agtatttaag ctttgagtcg tttgtttaat atcatttact
    25861 gcttgtacat tttgtgcatt atttacttgt tgtgttaatg cattcttttg tgtttggttt
    25921 aaatcacttg catgcgtaat cgcatttgtt gcttctgttt tcgcacgttg taagttttgt
    25981 tgaccatcta aagcgccttt tgtagatttc actgtttctg ctttttggtt cactgtatta
    26041 acatccatcg ttggattagt cgtttctcca atgacacctt tagctgcatt aactgcattg
    26101 tcatacgctt gtttattagc cgttgttgcg tcagtgtaat tttgacctga tttaatcgaa
    26161 ttattatccg caacactgtc ttttaatttt gtcatcgcat tatttaaagc tgtaatgtta
    26221 gacttgatcg tttcaagtgc agcatgattt ggtgcagcat taatttgttg cgtgaaatta
    26281 ttttgttgtg cttggtttaa gttagatgca ccatgtaatg ttgtaaacgc tggttgtcta
    26341 tctaatgtta aaccttgacg tgctgtattt aattgatcct tagcttggtt tgcctctgcc
    26401 actttatcat tgatattttg gaccgttggg ttgccattca acacttgatt aatttcagtt
    26461 aacttttgac gcgctgcttg taatttttgg ttgtacgcat ttaacgaagc ggtcgtcata
    26521 cctgttgtat ccgttggttg attaatgctt tgttctaatt gcgttttcgc agtttgaagc
    26581 ggtgctttat ctggtgttaa agcttgacgc gcatgatcca aatcagattt cgcttgattt
    26641 gctgcagacg tatttgtatt aatttgatct acagtaggtg aaccagctaa tacttgattg
    26701 atttgttgaa tcttattacg tgcagctgtt aacttcgcat tgtatgcatt aatagaatct
    26761 tgcgtcatac cagttgtact tgtttgcgta tcaatacttt gttgtagttg atttttcgca
    26821 ttttctaaag gcgccttatc tactgttaag ccattgcgtg cttgatcaag tgctgtttta
    26881 gtagcattcg ctgctgtcac gttttgtcga attgttgcaa catctggatg cgatgctagt
    26941 acgcgatcaa tttcttgaat tttagttcta gctgctgaaa gtttgtcatt aaatgcagca
    27001 actgatgcac ttgtcatacc tgtcgtgctc gttggttgat caatatcatt ttgcaactga
    27061 gttttcgcag cttgtaatgg tgccaagtct ggtgttaatc cggcaatagc ttgttttaag
    27121 ccattatatt tttcttctac tttagttttt tttgcggcaa tttgttggtc tgttgcgtca
    27181 ccattgttaa taacattttg tgcatttgtt gattctgttt gacccgcacg tttagcattt
    27241 tcatatgctt ggattgatga ttgtgtcata ccatcagtag ttactgattt attgatttct
    27301 tcatcaagtt tcgttttagc agttcttaaa gcactattat cagctaaagg tactaattga
    27361 ttaatagcta gcgttaatcg gtcatttaca cgatttacat tcgttaatgc tgtttgtacc
    27421 tcttgcactg atctaattgg cttctgaata atagcgttgg cactattttt agcacttgtt
    27481 aagtcacttt gaagtgcacg aatcgaatta ttgtatgcag tgatacttgc tggtttctta
    27541 cctgtcgttg tacctgtacg attcaattga tgtactgctt gctctaattc atgtgtatct
    27601 gctgttaaat tttgtttcgc ttggtttaat gctgttaatg cgttatcgac acgatgtttt
    27661 tcatctgaaa tttgttgtgc tgttgcgtca ccattatcaa tgacacgttg agctgctgta
    27721 atttcagatt ctgcttcacg tttcttcgca ttatagttat caatactttg ttgcgtcata
    27781 ccagtagttg atggtacttg gttcacagaa ctttgtaagt tatttttaga tgttactaat
    27841 tggctattat cttctttatt ttgaagtaat gctttagctt catttatctt agtttgtgca
    27901 gcttgaactt tagataatgc tgcgttcact tgttgtggtg ttgcacgctc atcattaatc
    27961 acttgttgcg cttctgtttt cgcagtattg atttgttgtt gcgcattatg aattgcatta
    28021 ttgtactgcg taattgtacc tggcttttta ccatctgtac tcactggatc atctaaatga
    28081 tttttagctg tgattaactc acgtttatcc gcttttgtga aaatggactc ttgtacttct
    28141 tcagtactgc tatcattgta agttactgtc acagggatcg tcgttgtact accaccagct
    28201 aaattagtag gcattgctgt gccattttta atcgttgcag tacgtttatt agcaacttga
    28261 actgcattgt taatttcagc tgctgttaca tttgaaccat aatcttttac aatttcagtt
    28321 gtgttgacag tatgtgctgc cggtgcagtt aatgtacttg gattactgct tgctgagtga
    28381 ccagtacctg ctttcggtgt aatggtgatt gctgaatttg gcttaatagt attggcattg
    28441 aacgtcactt taccagtatg tgcatctagc gttacatagt caggtttatt ggcgattgtc
    28501 cattgattat tttgaccacg aacagcatta agtgtcttac tatgttctgc accattaccc
    28561 actttttcag tgtaagcaat atccattgct tgagttggat taattaaatg tccagacggg
    28621 ttattaggcg tgatatcaac atgaccattt tgccaaatct tagtagtcgc ttggttcggt
    28681 tgaggtgcga caactgtgaa atcatcacta cgttgttcgt cactgattgt ttcaccacta
    28741 ccttgtgttg caacagcttg aattgtatca gcaggattga aagtaccagc tgcaacagta
    28801 ataccattat tcgttccagc aatacctgct acatttgctg cagatgcttc tttcacccat
    28861 ggactcgtat tattatgacg agtaaatgtc gtcacaacat taccattacg tttaataact
    28921 aatttatcag cgtatgtcgt tacgttaccg gcatgtgtat tcactgtttg gtttgctcca
    28981 ggtgcaatcg taattactcc tgctgccgtt tctgtaacag ttggtttcgc tggttgaaca
    29041 tcttttacta caaacttcgc tggtagagat gttgcaaatg tatgtccatt atagatgaca
    29101 tcatattttg cattaatgac ttgtgcagta tttggtttat tcattgctgc ccagtttgca
    29161 tcgtttgtac ctgttgctgc attattccat ttatacgtaa atccatctgt tggtaaacca
    29221 gtagcatttt gtatatgtgc atatcctgat gcttgcgtac cacttgctaa agtgccacca
    29281 actgttgttg tataagaagt ttgagggaat tcaaattgat atacattcac agtaacaggt
    29341 acacgttttg cagctgaaat acctggatat gttacatcga catttaaatg ttgaacgcct
    29401 gcttgttggt tatttggttg ttgtctattt gcccatactg cagtgatacc atttgtgttt
    29461 gtatttggat caaatgtaat gtagtttatc gcatccgttc cattagtcaa attttgacct
    29521 ttaacatcac gagatggcgc tttggcatta gcaactggat aaactttgac aggtacttct
    29581 acattacgtg tcccttgacc atttggtaat gttacaaccg cagttttatg agtgtttccg
    29641 actgtattct tccatgtatc tggattatca tgccatgcaa ccgttgcccc atgtggtgga
    29701 ttttgaataa atcgttctac atgaccgaaa tcaaaaccgt cgccaccttt aataaataca
    29761 gcgccttcag tagtcgcttg taattgaggt gtcacatgta cagaagcact gtcatttgaa
    29821 tcaacagatt catttcttgt tacatctata gctcgtccat gttcgtcttg tgtcgtataa
    29881 gttacattat tcattgaaat cgaagatctt gctttaattt caccatttgg taacgcatca
    29941 cttacagtaa ctactgcagt atttccacta ccataagtcg tgttagtaat tgttaatggt
    30001 gtattatctg ctttaaataa tttaatagaa gaactactta aaacattatt aatcttgatt
    30061 tgttggttag ttaagcctgc tttgtatgtc acagagtttc cgtcaattct cggtggatca
    30121 ggcttaactt tagataaaat cttgataatt tgtgtttggt gattatcata agtaactcgg
    30181 atatcccttg ttgaaatacc tactacatct ttacttggtc catatgaatt tgtaaattgg
    30241 aaattcatat gttgcgcatt tgtcgaccaa gaattattta ctggcttaac atattgtttc
    30301 gcatcataca tatctgaaac acctgggtat aagacacctc tggctgtttc aaagacttgc
    30361 tttggtacag ttcttaccac tttatatgtt gctgttttcg taattggggt tgtttcacca
    30421 tcaattaaga tatgtgcagt tacattgata tcttgaccta tagttgtgtt atttttgttt
    30481 ggcgcttgac cacttaccca tgttatcgtc gcatctggaa tggcactacc attcgataat
    30541 ttaaagtaat cagatgcact tgctccatta cctgttggga agtcttgccc ttgtacagtg
    30601 taatgcgaat gtgcaacgat ttctggaata acacgcttta caggtactgt cactgttgat
    30661 gttgttccat cttgataagt aacagttaca gttacttgtg cgttatttcc tgtacgactc
    30721 acattactca ctgttttact agtgatttca tttgcacttg ctgttgcata gtttctattt
    30781 ggtgcattgc ttgtaaacgt taatgaatta ataattgctg tttgatcagc ttgcgatact
    30841 gtcgcattat tcgaaatatt ggcaattctc actggattag cagccgttga tgatgttcca
    30901 actcgatatt tatcacgtaa aggtttaatt gtgacattga atgacgttgt tgcagtatta
    30961 ccgcttgtat cagttgcaac caaattaatt gttttatttg tagtagatgt cacatttggt
    31021 gctgttgctg aaacatgttg atgattatta tcaacagtgc ctgtcaattg tgatgtcgtt
    31081 ggtactgtta cagattgcac accaaaatta tcattcgctg taatcgtatt ggtaaatgtt
    31141 tcaccagaga atacttgatg atttgtgtaa ttacctactg aaatcgttgg atttactaga
    31201 tcactaggta caaaataaat gttaattgct tcattcgtat tgtccgtatt ttgatttaaa
    31261 tgttcaacat attgttttgg tccatatggc gataagtata actgcgcttt ataaactgca
    31321 tcagctgcat tatgcgtaga attatttttt actacatgat ctattgtaaa tgcacccgca
    31381 ccattagctg ctgattcatt aacactaacg acagtcgagt tactgtggtt cgcacggaca
    31441 tttgaatttg taacaggttg cccaccaaat ccatttgaag gattaataat atccattatt
    31501 tgaatgactt gaccattcaa cgtaaattgt cgttgaccat cagatgttgg atttgattgt
    31561 gaataaccag ttgttttata gtttggtcta ccaccagctt ctgcttgtgc tttttcatta
    31621 ccagcaatgt tattacgtaa tccaggcacc gttgttgtcg cttttaacaa agttaaaata
    31681 tcattcgtcg taatttgcgt acctaatgtt gcatcatagc gataaattaa tgatttatga
    31741 tcatcagacc atgataaacc accatcagtc ggtctatttg caatttttgc gtttgtccat
    31801 gtcactgtat aatcttgagg gaagttaacc caacgtaaga agttcatatt attaagattt
    31861 gatgtgaacg atttcgttaa tcgaccttta tttatattga cagtaatggt actcacatta
    31921 gaaccattcg gtgtatttgt tactgaaata tctccttctg caaatgaaat cgcatctcta
    31981 ttggcattga taaatgcttg tttcactttt gaaatttcat cagtagttat atcatatgtt
    32041 gacatacgat atacacgttg atcaccttct ggttgtttca catcaatgat tttcaaagtg
    32101 atatagaaat cttcagtgtc tttatgatac gcatttgtag caactatatg atatagctgt
    32161 gtgccagcgt tcatttgact aacttcttgc gcagttggaa tcgttctatc cgatgtaatg
    32221 tttgtaaatg aactgcttgg aatcgcttgt ccattactta aagtgaaata ttgtttgata
    32281 tctccaatat taaaagattg ccctttagca attgtaaccg ttgccgtttt agcagcatta
    32341 gcagtttgat attcaggctt aacaactggt tcgtatgtca tcacgtttgt cgcatcaagc
    32401 gtagttgatg acccatcacg atattgcaat gtgacattac catgattatc tactgtaact
    32461 ggatttgatg aagctaaata acctcttata ttttggtttt tattcataaa cgcagtagta
    32521 atacgttgct tttcatcacc agttaaagca gtcggattta caacgacaac tttttcagaa
    32581 tttcctaaga caattggatg cgcatcatca ctaattttac ctacatgcac tgtcacatgt
    32641 ttagactgac tatcattcgt cgtattattg acattgtcgg ttgctgacac tttgaatgta
    32701 atatcactat tataggcctg attcatagag actctacctg aaatagctaa tgagccgttt
    32761 ttcttgtcgg atttagttaa atttgatgtc caaccacttg gcaatgtcgt aatagttgta
    32821 ctttttaatc cagaatgtct atctgaaacc cccatagtaa agctaacttc atcgccacga
    32881 taatatttcg catttaatcc aactgggtta ttaattgtcg gtggaatatc atcaggtaaa
    32941 atagttactg cattactcat atttgaggca actacattat tgtaaaatat taacgcccta
    33001 atttgattac ctaccggagg tataaatact acataatctt ctggtcgtat cgttatcgtt
    33061 gaatgaaccc catcaggtgt acccgtcgca atttttgtat aacctgatgg tatttgatct
    33121 gcctcagaac caccatctct tgtttgagac gtaggtataa ctaaatatac taatgcagat
    33181 ggatccaatg gaatatccgt aacggttaca ggtacctttt gtaatgctgt acctcttaat
    33241 tcttctgctg ttgtagtaat tctaggcgta tgtggcttaa cacgaatctc gaataattgt
    33301 tctttacgtc caaatcgtat tgttccaaag taatcgcctg gtaataaatt accaaccagt
    33361 tcaatattac gttctctagc attattagtt ctatttgttg aattctcaat gtgtaatgta
    33421 cgtggaatac ctgtaatttg ccaatcttta tctgtaaagt tttcattact tgttaaatta
    33481 atacgtaacg gttgtataga tgttacacta ccacctaagt ttactgaagc attcccttga
    33541 ttacctgcat atacatagaa aaccggatgt ccagcttgtt ttgaagcagg tttatcagca
    33601 atattttgac cgtcatcatt tttaaagaat ttaatgttat gttgaatttc aggtaatact
    33661 gttaagttaa caggcactaa agtttcaccg ttaccaaaag catcaggata gtcgaaacca
    33721 atcattaatc gtattgggct gccagttcta gctgcctgtt gcaatttatc atcagtaaat
    33781 aattgactta attgatattc atttaatgga ttgactccac ccggtctaaa agcgttagtg
    33841 tttgcactag ttggataaaa acctaatact ttattacttg gtgtcttaac ataacttaat
    33901 ggatcatcca taaagtcatc tatatttagg ttcgtacttt ctaatacctt ttttgtttgt
    33961 acatttcctt gactcacttc tactcgttta gttgtaccta tacgataagt acgattaacc
    34021 acttgtctta aaccattaag acgttgttga tagtcttgtg ttgattcgac atataactga
    34081 ctatacataa ataattgatt gtattctaaa gcacgcccac ctgctgcata gtacagctta
    34141 tcaattgttg gaccttgcgt tttaaatgaa atcgtataac ttgcatcacc tttaccaata
    34201 aatgtataaa cttttggtac accaggtgat tctgtcggcc tttcaccatt aatatattcg
    34261 aatgtttgtc taccaaaagc ttttgtagca ggtgttgctc cagcttcact aaaatagtca
    34321 cttgcacgtg ctaaatcctt taagctatta atatggacag ttggccaatc ataaatcacc
    34381 tgcccactcc tatttctaat tttgtagtcc cacgaacgtg agtcgtttac cccatatggc
    34441 cacatttctg gaagtggttt gttcgcaccg gctcctgcac cgttactcca ttgtacattt
    34501 gtgccgtctg cattcactgt tacaaactcc gttcgaccaa ctggagtttg tccggccggt
    34561 agtgcaaacc agtagatcat attttcatgt ggtgcatgac ctttgttaaa cttaatcacc
    34621 cattgataac cgtctccagt ttgctttgct gtaataagtg cgatttcttt tgcgtctgat
    34681 gcttttaacg tattccatgg cgtagtaggt aaattatatg gtgccccctt actagaagca
    34741 ttaatatatg tacctacagg atacccagaa ccatcattag cattttcaac gtaatatgat
    34801 gagtttgcgt tttgatagtc tgatactgat cttggttctg cagctcttga ttcaatcgaa
    34861 cgcgtttgaa ttctacttga gcgtcgactt gtttgttgac gttgaggcat tgtacgaatt
    34921 aaagcaacag cttgttctaa ttctttgtat gcaagttgca agtctttatt gtttattgga
    34981 tcattactac ctaataacgt attgatcttg tcaaaaatgc cttttacata gttaaatgtc
    35041 ccagaatctg cattttcgcg gtcaataaag cgataatcat ggtaattagc atcaaaaaat
    35101 gcttgtaatt cattacgatc gtgttgtacg ttaccactat ctgatgaatc tgaagctgtg
    35161 actacatttg cttcatgact ttctacatgt gtcgctttat tgtcgttttc ctttttgtta
    35221 gcagcgcgac tttcgctttg ttcatgctta cttgccgctt tagccgattg tgttggcgta
    35281 gcagctgccg tatctttctt tgcttgctca ttttgagata ctggttggtc atgttctgtt
    35341 gtagatgatt gtactgcact agcatctaca tggtttgttt gattattatt tggttgttca
    35401 ttttcaatag gcgcagataa tgattgacca ttttgtgaat tttgaacttc tacttctcga
    35461 ttctcagtct gcgtatcacc attttgttgt ttctgtttta atgcactagc tggttgattt
    35521 gtctcagcag cttgtgcttg tgatgtgttt aatcctagaa atactaacgt cgcaatgacc
    35581 gtcgagaatg ttccaacggt atacttacga atactaaact tttgaatttt atcacgataa
    35641 ttcacagcta ttgttcactc cttatcttgt tgttatgtct ctgttgtgtt gtgaatgtat
    35701 ttacatttga tttcatgtgt attgtaatgc tcattccatt ctcatttaaa aatttaaaac
    35761 aatgacgatt caatacaatt tcgcaatcta tctaaaaagt attgaaatac attacaacta
    35821 tgtgttgaac aatttattta ttctccagaa tataataaca caaaatatat tggtaggtaa
    35881 attgaatttg tgacaattta taataattat aaataaagta gtgtaaaagg gtaataaaaa
    35941 accgacctta tgtagaatca tttataatga ttacacataa gaatcggttt atatttttgt
    36001 ttaatattgt agcgaccttt atgatttcaa tttaattctc aattatcata attgagtgtc
    36061 gttttgttta ggcacaagta acaaaatgat aacgaatgat aaaattccca tacctgcgtt
    36121 taaccataat gcaatcattg caccagtatc aatgtttgtc atatttgata cgattgcata
    36181 aactgcacca ctcaatgcga cgccaaatgc tcctcctaat gcagaagcca ttttatagat
    36241 acctgcagct acgcctactt tttctaacgg tgcatttgca attgctgtat ctgttgatgg
    36301 tgtagcatat attcctagtc ctaaaccaaa gaataaataa cctataatac aacaaatgac
    36361 atataatatt tctggcaaaa atgttaatga aatgagacat tctccgacaa taagaactgc
    36421 tgttccaatt agcattggtt tcttgcatcc gaatgtttga agtaactttt caccaacacg
    36481 aatcataatt agtaccatta ctaaataagt gattgataaa cttcctgctt gcaaggaaga
    36541 atatcctaaa cctctttgaa caaatgtgtt ggctactatt aatgttcctg caacaccatt
    36601 taacaaaaag tttgaagctg ttgcacctgt ataagcttta tttttaaata atttaaaatc
    36661 gattaaagga tttgtagcac gcttttcaag aactataaat aaactaaaag atacaattgc
    36721 aattgctaat atagtgataa aaagaagtga ggtaacacct aattctgatc ctttagtaat
    36781 taaaatattt aaactgagaa gcataatgac taaaagaacc agacctttaa tgtcaaattt
    36841 atttagagaa atcgatttag atttagtttc aggtgtgcct ttaataagaa acagcgcaat
    36901 taatgaaatt ataattgata agatgaaaat ccaacgccaa cctaaaagtg ttgcaactgc
    36961 acctccaaaa aatgaacaaa caccagagcc gccccatgag ccaattgacc aataacttaa
    37021 agcgcgttgt ctatctttcc caatgtaata tgacttaata atagacaaag ttgcaggcat
    37081 aatacatgct gctgaaagtc cttgaattaa tcttcctata ataagtaata aaggaatatt
    37141 tgaaataatg attaataatg aacccaatat atttaagata ataccaatgt tcgtgagttt
    37201 aattctgcca tatttatcag caagaccacc tgctcctact acaaacattc ctgaaaataa
    37261 agcagttata ctaacagcga tattaaccgt tccaatatct gtattaaaac tatcttcaag
    37321 tattggtaca acattaacca atgattgtgc aaatagccaa aacgttatta cacttaaaac
    37381 aattcctatt aacaacttat tattgccttc aaatgtctct cttgacggct tttccatctc
    37441 tatttgcctc cctatacttt tgaaaaacag acaaattcca taacagaaat ttgtctgttc
    37501 gatagaataa ttgaatggtt tttgatttta atgtgcaaaa ctttaaattt attagtagtc
    37561 cattcaatta ttctcaatgc tagttatctt accttatgct gctttttgtt tcttcttata
    37621 gtaataaact ggtataccaa gtgctgttat tagaatccca ataattgcca atataaattg
    37681 tgtaaacagt gtattaatta atacaaatga tcctgccaaa atagcaatta aaggtatgat
    37741 tggatataac ggtactttat atggtcgttc catatttggt tcacgttttc ttaaaattat
    37801 taccgcaaca aatgacatac aatagaacaa ccaaataaca aaaattaaca tgtttgtaat
    37861 tgtatcaaag gcacccattg acatcatgat gatagcgatt ataagttgta taattgcgcc
    37921 aaaccatggt gcaccagatt ttgttaattt tgcgaataag tggctaaatg gcaataattt
    37981 tctttcagcc attgcatatg gtacgcgcat accagtcata gtatagccat tgatcgtacc
    38041 ataaacagat attaatatac cgattgtgat aatcttaccg ccattttctc caaataatat
    38101 ttttgatgta tctgatgctg catttaaatt tcctgcaagt agttctattg gcagtgttaa
    38161 taagaatgta gcgttaatta ataaatacac agccataata caaccgatac caactgaaat
    38221 cgctaaaggt aaatcgcgtt taggattttt aagttctccc gcaacatttc ctacatgaat
    38281 ccaaccatca tatgcaaaca tagttgctaa taaaccacta ccaattgctg taaagaaacc
    38341 atttcctgaa ttacctgtag ttggaattaa tgaaaaagtg atgtctcctg attggaaaat
    38401 accaaagata acgataacaa taattggaat taatttaata actaaggtaa ctgattgtaa
    38461 aattccgcct gcttttgaac ctaggaaatt tatcaacaca atagataacg cagatgcgat
    38521 tgctattggt attaatgaac ctatagataa atggaataaa ttaattaact gtgtcgcaaa
    38581 tacgatagac aatgctgcta cgtttgccgg aaaataaata aatgattgtg cccagcctga
    38641 taggaagccc cagaaatcac cgtatgtata ttctatatac tttgttaagc cacctgtttc
    38701 agggattgca gcagcaagtt ctgctgctgt taaccccgca caaatggtaa tgatgccgcc
    38761 taggaaccat acaaacaagg ccattcctgc tgttcctgtt acttctgtta cgtttgatat
    38821 tttaaagaat actcctgaac caataactgt ccccataaca atagcaaacg ctgagaagaa
    38881 acctatattt ttttgtaatt ctttaccatt tgacataatt acacctcatt tttcagatta
    38941 aatgttttaa cctaccacac ccttgcttgt atctgcaata ttcagtccat gttcaatgac
    39001 acctgaaact ctagttaagt caacattccc gcctgaaact aaagcaacaa catttttatc
    39061 ttcaagccat ttattgttta tttttccact taaaattgca gctgttggta atgcgcctgc
    39121 accttcagta ataattttgg cacgctgcat taaatctttc atagcatgtt caatttcttc
    39181 ttcagtaaca agaataaatt catctactaa atgtttaact acttcatatg tttgttcacc
    39241 aggaactttt acatcacaac catctgctat tgtgctatcc actcgatgtt cagttaaatc
    39301 tctcttatag aaagactcag ccataccatg aacattctca gattgaacac cgataatatg
    39361 aattgaaggg ttaaatgatt ttaatgcggt ggcaatacct gcaattaatc ctccaccgcc
    39421 aactggtacg atgactgtat tcacattcca aatatcatct aaaatttcta aaccaattgt
    39481 tccttggcct gccattacaa acttatcgtc atatggatga acgattgtca tgccattttc
    39541 tttcgctaat tcttccatat aaagtctagt ttcgttaaag tttttacctt ttaaaataac
    39601 ctttgcccca tagcctttcg ttgcttgttg tttcgcttgt ggtgctgttt caggcattac
    39661 gatagttgca tcaatgccta ataatttagc tgttaaagca acaccttgag catggttccc
    39721 agcagatgct gcgataatgc ctttttcttt ttgttcatcc gttaagtgat taattttatt
    39781 gctagcgcct ctaaatttaa aagatcctgt gaattgcata ttttctaatt ttagaaatac
    39841 attcccttta gttatacttt ggcttaaata cattgattta attagaggtg ttcgacgaat
    39901 aaatggttta atgcttgctt tagcttcttc gatatctcct aaacttacaa tatgtgctgt
    39961 ttgtaatgta actgtgttgg ttgtcataat aattacatcc ttccgttttg taaattttta
    40021 gtccaaacta taatgctata tttaaaatat tcatttcact caatttcatt taaatcacta
    40081 tctattcgat aacatttaat atttctttat aatctaggtc atgtgatgta gctaatcctt
    40141 ggtttgtcac ttgtccttgg taaatgttta ctccagtact taaggcttca ttatctttaa
    40201 ttgcttgttc taggcctttg tcacaaattt ctaatatata atcaatattt ccttgtgcta
    40261 atgccattgt tgaagttctt gggactgctc ctggttgatt tggtacacca taatgaatca
    40321 caccttcttc ttcatacacc ggatcagaaa ttgtagttgg tctaattgtt tcaatagttc
    40381 caccttggtc aatagctata tcgattaata ctgaaccttt tttcattgat ttaaccatct
    40441 cacgagtaac caattttggc ggtttcgcac caggaattaa aattgtagaa ataaatacat
    40501 ctgctttttt aatttgttct gctaaatttt ctggtgttga tttgactact gtgacatctt
    40561 tttctgcata catatcttca agatatttaa tgcggtcatc gtttaactcg ataatgatta
    40621 ctttagcatt tagtcccaag gcaacatttg ctgcatttgt tgctgctacg ccaccaccga
    40681 aaatcacata tgtactacca ggtatatcca cattttcatg tacaccagtc actaaagtac
    40741 cttgaccacc atgttgtgct tcagagtagt aagctcccat aattgctgag cgttgacctg
    40801 ctatagcact cattggcgct aataattctg cttttccatt ttttataatg gtttcaccac
    40861 taatcgcagt tacaccaact tcttgcattt tttctactat ttcttttgaa gatgctagat
    40921 gtaaaaatcc ccagataatt tgattctttt tgaaatattg atattcgctt tcatgaggtt
    40981 cttttacttt gataacaaga tcagcttccc atgcttgttc gtgagttacg atcttagcgc
    41041 cttctttttc atacatatcg ttagaaaatc ctgaaccaat gccagcattt ttttcaacaa
    41101 ttactttatg tcctgcatcc gttaacttac gcacattttc gggtgtgcaa gctacacgtc
    41161 cttcaccttg ttttaattct ttgactactg caactaacat ttgcaacact cctctttcat
    41221 ttgtgaataa tttcacaatt attttatcct ttggttatga atatgtaaat accattaatt
    41281 aattattcat attttatcta taatttgtta ataaatattt aatatattat tattattctt
    41341 aaatattatg tgcaattata ccactatatt tatttcttat actccatttg atttatagtt
    41401 gtattaatac attgactcaa aaaataatta atcaaatatg ttttttagat taataaagtt
    41461 gtaactgtac tattttgtag tgtatggtaa tttatttgga tgaaatataa ttctttatac
    41521 tctattgatt aatcaaatat gtatctatca aaattcggct tatttattca ctctgacata
    41581 ttcaaatacg caaaaagact atttctacct tgtatcgata gaaatagtct ttttatactt
    41641 aattggttta atttttcata aaatcttata ctttttaaaa atgggatgaa atatattttc
    41701 ctgaaacatg taattcatgt tcgttacaaa ttgataaaat atgattaagc gttgttgcaa
    41761 atgacatttt ctcaaaaagc gcttcactat caattggtac ttgtgtgtga atttcagcaa
    41821 gtcgcttcga taaatatagt tcatctaaat tgtcatttat cttattacgt tgaccagctg
    41881 ataatgcatc aatgttttca accacatttt caacgctttg atattgctga attaacttaa
    41941 tagccgtttt ctcgccaata cctttaacac ctgcatatcc atctgctgta tcacccataa
    42001 acgctttaat atcgattagt tgttgtggtt caagagcata ttcttcgtta aaacgatgta
    42061 atgtatatct attataaatg ttaaaacctt ttttaattag ccagacttca acattgtcat
    42121 taatacattg cagtaaatct ttgtcgcccg taataatata gacatcgtta tcagttgaat
    42181 attgttgtgc taatgtacct ataacatcat ccgcttcata gtttttaacg ccaatattta
    42241 caaagccaaa ttgctctgaa atttctttaa cataatcaaa ttgtggtatc aattcttctg
    42301 gtggtgcaga acgattttgc ttataaccgt caaacatatc attcctaaaa gttgattgcc
    42361 ccatatccca acatacagct acatgtgtag ggcgtatttc atgtattgcc gaaaagatat
    42421 gacgcacaaa tccttgtatt ccatttgtag gtacaccttg tgaattgtac ataaattgtt
    42481 tatgaagact tgtagcgtag aaatgtctaa ataatagcgc cataccatct acaagtaata
    42541 ttttatttgg catttaaaac gttccttctt tcttaatata aaatagtttt cagttgttga
    42601 tgtttatctt tcaattgatt gataagtgtt gtatctaaat caaatgctaa taaatcgttt
    42661 atttgcgttt gaatattagc ttcaaaactt tcaaattgcg attcagcttc tacagccatt
    42721 tgctttacat ataattcaag ctgttgcctc aataatccca tttgtggttg taataatcct
    42781 aacgtacttt gacaaattga ttcatgtata tctctttgcc catttggatt caaaatttta
    42841 cgttttgtta attgtttagg caatgcattt agcatatcgt tgaaatcaat gtgcaataat
    42901 ggttgctcta tattagctga ttcaaagtta aactgaggat taacaatgac atgtaaatct
    42961 gctaattgtt gaacgattgg tgcaatttgc tcagtgagtt gatttttaaa gtattttttt
    43021 atacgttctg taattaaaga ttgctctaaa aacaatcgtt gatgaatttg atctaagtat
    43081 actttcgtag acactttctt ttcttcatta aaatcactat tttgcgtcat ttgagaatta
    43141 aacactgatt taatatcgtc tagtagttgt agttttaaac gggcatttaa atgataaact
    43201 tgttcttcaa cttcgttgtc tgtatgttgt agcgtcgcat caattaattg atgttgtaaa
    43261 cgctgtttat ctttatagac tgttaaccta tgttggcgac gtgaaatatc agctttattt
    43321 gtttcaaatt ctgtaatcat ctctacataa gaatgatcca tttgttgaag ctgatgaatc
    43381 atttgttgtt ctaaaattga tttagattca acatcaacaa attgttgtat gctttgtttt
    43441 aattgatcaa tgcccttatc ttcagcttgc aatgcatttc gacttgatac agcaaaaatg
    43501 tctgattgta agtgtacttg tcttaatgca tctgatacat atgtttcaac cgcttcaaga
    43561 tcatcttgac tttctgctaa atcagcagca ttaattacca ttttaaatgc ttggttttca
    43621 ttcaactggt tcatatcttt catgtgttct ataaacgcct tgtcattatc agtaaacgaa
    43681 tgattaaaat aacttacata caagattaag tctgaagaag ttaaaatttg ctcggtttca
    43741 tttgtatgcc tttggttatt tgagtgtagc cctaatgaat caacaattat tttacctttt
    43801 aaccaatcat gcattaatgc aatgtgtact gttttaacaa atgttgcgta ttcatcttct
    43861 gcactccact ttttcaattc ctgttgatta atggcatgtt tttctccatt ttccaacata
    43921 ctgacataca aattataatg tttttcaact gcatgaacaa aagcgagttg atttttattt
    43981 aaatgtgatt ttaacttttc taaatctgaa ttaataaagt cttctatagt tgaaaaagat
    44041 atatcttggt attcaactac tgcattaatt tcatctaata attgcgattg tgattttagc
    44101 gttatataac tctcgtctcc ataagatatt tctgtagtag ctgctgtagt aggatttgga
    44161 gaactaacta aaatatgctc gcctaataat gcatttatca aactactttt acccgcacta
    44221 aacgttccaa atacacctat ttttattaat ttattatcta tacgtgttaa tgtttcgtgg
    44281 atatcctgct ttgtacgctt gaacaaaggc acatccgaaa ttatatcaag tgctttttga
    44341 atatctatag acatatttgt tgtagctgta ttctgattta gctgctgatt atcacgatta
    44401 tccttagcat tatcagtagc cacttgatat gttgtctctt gtcgatctac taatttatct
    44461 agagattcat ctaaatgaat atagtaatga cgataattct tagtcgtcaa tgactgacgc
    44521 agctcattta attctgtata acgttgatat tcttttaaat catcactttc ttctgttggt
    44581 aattcatctg cctgcacatt ttctattatt tctttaaaaa ttggtgttga ttgttgttca
    44641 acatatttct taatggcttt aaccacttcg tctgaaaatg taagtacata agtattgctg
    44701 attgatgttt gtggttgata taaaccttca atcatttccg gcttaacgtc ataatgctga
    44761 tttaatactt tatctgaagc ttctttttta ttgataaaac gcgtaacaaa tgacatatct
    44821 tctcgcattg gttgacgaat ttgttgatta acatgttctt gtaacgcatc tgtcgctgtt
    44881 aataatcgct gttgttgaat ttgaagcttt ttcttctttt tattaaaaag gccaccaaca
    44941 ttaaagtctt gagacatact ttccaaataa cttcgtaaca tttctctcat attatgcggc
    45001 ataatgtatg cattttctaa aatattttta cgcttattct ttaaaaatgc cattaattca
    45061 tctggattat ttaaaagttg cgcctcttca ctaattgctt gatgttgttg actatttaaa
    45121 aatgcttgtt caaactctgc ttcttcgata cccaaatctt ctagtacttc ctgaatttca
    45181 gactgaatgt aatctagctg agcttcggta atatattcaa ccgttcttga tgtataatcc
    45241 tctattgttt ctctatgttg atctaatgaa actagataag ttgataatgc tccaagttca
    45301 ttttcagagt gatcaaattt cgatacataa aaggtgtgtt ctaatttaat gtcccaatct
    45361 gcaattgatt tttcaactcg agatttaaac gtagcgaatg acaattcatc gtcttgatgc
    45421 ttgtcaattt gattaatgat aaacacaaca ggtattccaa catcatttat atgcttcata
    45481 aacttaaagt taagttcaga ttgaacgtgg ttatagtcaa ccgtataaaa tatcatatta
    45541 cttgtataca tatattgttc tgttattgac tgatgtgatg caacatttga atcaacacct
    45601 ggtgtatctt gcaacgtaaa cccattttca aatttagctg attgaaaatt aatttctaca
    45661 gattcaacgt cgacattttg gcgattcatt tcccttactt catcataatt agataatttg
    45721 gcatacgttt gattcggcaa attagcaata atatcgtgat tgtctgaaac tgacacgata
    45781 gcagtattac ttgtcgttgg tacaggtgaa cttggtaaga tatcttgttc aattaataaa
    45841 tttatcagtg tcgatttacc tgcagaaaaa tgtccaacga acgaacatgt atattgctgc
    45901 aaatatactt tcttaattac ttggttaatt gtatgtaaaa gtgcttcatt tcgcgacttt
    45961 tcaacttctt tttttaattt atataaaaga tctaattgtt ctttattaat catgttactt
    46021 tcccctttgg ttcgttcaca tagaaatgct tgcttgttaa aacgaattca tacaatatcg
    46081 atgtccattt aaacaaaggt cgttgaatgg ctcgcattgt attatgttta aattttatca
    46141 tagctgcttg ataaaacata tgaataatca attttcttca aacattccaa cttaaaagtt
    46201 catttttcta aaaatcacaa atcattgtta aatttttcta gaaaataaat attgtcacag
    46261 tttgttcaca cacgtcacag aattgacacc gtataatagc caattgggag ggaaatatga
    46321 aaaatacatt taatccatta caaagattac atttctacgc agcaatattt attgctccac
    46381 tgttaatcac tttaaccatt tcgggcattg gttatttgtt ctttccagaa gttgaaaata
    46441 atatttataa gaatgagttt tttggtgaca gtgatgtcaa aacgcatcaa acattaaatg
    46501 atgcagtaca tcaagttgaa cgacaatatg aaggattctt tgtaagtaaa gttagcatac
    46561 ttgatgaacc atataacaaa cgaattacac tgagtgatat ggcaggaaat cagcgttacg
    46621 tctttctaga tcataacaat caatttgttg cagatcaaaa tgcgaaacat acgtattcta
    46681 atgtgatgcg aagtatacat agttctttgt ttactgaaaa tactattatt aattatttag
    46741 ttgagttaac cgcatgttgg acgatattca tgattttatc tggtacttat ttactcatta
    46801 agaagcattt aatttctaac aaaagtaagg cacttcattg gcaaaagtgg cacgcaatga
    46861 ttggaattat cattgcaatt ccagtatttg tattagtctt aactggattg ccatggtctg
    46921 gttttatggg cagtaaaatt gccggtatga tggacacaaa cggtgacctt ggtcaaggtg
    46981 aattagcgat taatccacct aaatcagatt tgaatgaatt accttgggct acacgtaaaa
    47041 ataaacagcc agcttcatcc gaaaaaggtt caagtggtca tcatggtaat gcagcaatgc
    47101 ctcaaactaa attagattat caaatatcta ttgataaggt cgttgaacag gcgcaaaaat
    47161 ctggtattaa aaagccgttt tcaatcgtat atccaagtga taaaattggc acctttattg
    47221 tatctaatac tagtaattca ggtgttactg ggctagatgt atctccttac aaggaacaaa
    47281 cactttattt tgatcaatat agcggtaaaa agctaggtac gattaaatat gatgactacg
    47341 gtattattgc taaatggttt acgtggggca ttccgcttca cgaaggtcat ttattcggta
    47401 ttgtaaacaa aatcattaat ttatttgtat gtatcgcttt attagtagcc attggtatgg
    47461 gatttgtctc ttggataaat cgtacaaaaa atactgcagt aaaagtacca catcgcgtaa
    47521 aaaaaaccag catctatatc actcatcata tgtttaattg tattaggact attaatgcca
    47581 ttatttggat tatcacttat cctagtattt ataattgaat taatattata tattaaagat
    47641 cgtcgtgcta aacaataatg cacttaaagt tttgaactga cgaaatttac aaaatgattc
    47701 tctaattact taaaacaggt tctataataa atcgaactga tggagcttgg gagataaatc
    47761 aatgttctag ggtctataat attatattgg cagtagttga ctgaatgaaa atgcgcttgc
    47821 aacaagcttt tttcaactct agtcaacctt gccggggtgg gacaacgaaa taaattttgt
    47881 aaaaatatca tttctgtccc actcccatct gtccgatttt ttgaatttga agataaaaaa
    47941 gcatctaaaa cgtataagaa gattacttta cttcttccaa attacgttat agatgctttt
    48001 tattaattca tatgcatgtc gtgaattttc atcattaaac cgtgtgggac gtcatcatgt
    48061 ttaacaatct cttgtttata aaatgtcttc ccatcatctt tagaaactaa ttttacaaag
    48121 tctccttttt tcggtttaaa atcatttgat ggttctactt tgatattatg tttgactaat
    48181 ccatcttttt cactgacaac tttttctgca tttgtattac tattcatata accaaaataa
    48241 gtcgtttttt gactagaaaa aatcattaaa catacaacgg caataccaat aacaaccaat
    48301 gctatagata aacctagacc cattttttta cccatttata gactcctcca tttttcaaaa
    48361 gtacctatta tcatgagaac ataattatta ctagttttag tcacgcatta gaaacacttt
    48421 agttttcaat agttttacgt tctgtctttt taccccagta ttgataataa gtacattcta
    48481 tatatccatt aaataactta cgtcgcttcg ttgctttacg atctactaaa tattcaaatt
    48541 ctttattact tgttaaaatg tatgtagata aaaatggatg ttgtttcatt agtttaccaa
    48601 tataacggta catttcttca acttcttcac gatcaccaat acgttcacca tatggaggat
    48661 ttccaattaa cgcaaccggt tcttctgtat caattgttaa tgtattgaca tcttttacac
    48721 taaatttaat aatatcagac aaccccactt cttcagcgtt acgcttagca atctctacca
    48781 tttcaggatc gatatcagaa gcatatactt cgatttcttt atcataatca gccatcttat
    48841 ccgcttcatc acggtaatca tcataaatat ttgcttgcat gatgttccat tgctctgata
    48901 cgaactctcg attaaaacca ggtgcgatat tttgagcaat taaacaagct tctatagcta
    48961 ttgtacccga accgcaaaat ggatcaatta aaggtgtatc acctttccag tttgcaagac
    49021 ggattaaact tgctgccaac gtttctttaa ttggtgcttc accttgtgct aatctataac
    49081 cacgtctgtt caaaccagaa cctgatgtgt cgatagtcaa taatacatta tcttttaaaa
    49141 tggcaacttc aacggggtat ttcgcacctg attcatttaa ccaacctttt tcgttatatg
    49201 cgcgacgtaa tcgttcaaca atagctttct tagttatcgc ctgacaatct ggcacactat
    49261 gtagtgttga tttaacgctt ctaccttgaa ctgggaagtt accttcttta tcaataatag
    49321 attcccaagg gagcgctttg gtttgttcga ataattcgtc aaatgttgtt gcgttaaaac
    49381 gtccaacaac aattttgatt cggtctgctg tgcgcaacca taaatttgcc tttacaattg
    49441 cacttgcgtc tccttcaaaa aatatacggc cattttcaac atttgtttca tagcctaatt
    49501 cttgaatttc cctagcaaca acagcttcta atcccatcgg acaaactgca agtaattgaa
    49561 acatatatga ttctcctttt atacaggtat tttattctta gcttgtgttt tttatacatt
    49621 tccaacaaat ttaatcgctg atacattaac gcatttgctt atcattttaa aacaaagcag
    49681 tgtcattata tcaagataag gcgttaattt taagtgttct tttcatgaaa aaagctctcc
    49741 atcatctagg agagctaaac tagtagtgat atttctataa gccatgttct gttccatcgt
    49801 actcatcacg tgcactagtc acactggtac tcaggtgata accatctgtc tacaccactt
    49861 catttcgcga agtgtgtctc gtttatacgt tgaattccgt taaacaagtg ctcctaccaa
    49921 atttggattg ctcactcgag gggtttaccg cgttccacct tttatatttc tataaaagct
    49981 acgtcactgt ggcactttca aattactcta tccatatcga aagacttagg atatttcatt
    50041 gccgtcaaat taatgccttg atttattgtt tcatcaagca cgaacactac aatcatctca
    50101 gactgtgtga gcatggactt tcctctatat aatatagcga ttacccaaaa tatcactttt
    50161 aaaattataa catagtcatt attagtaaga cagttaaact tttgtattta gtaattattt
    50221 accaaataca gctttttcta agtttgaaat acgttttaaa atatctacat tatttgaaga
    50281 tgtatttgtt gttgtattat tcgaagaaaa acttttattg tcctgaggtc tagatgttgc
    50341 tacacgtagt cttaattctt ctaattcttt tttaagttta tgattctctt ctgataattt
    50401 tacaacttca ttattcatat cggccatttt ttgataatca gcaataatgt catctaaaaa
    50461 tgcatctact tcttctcttc tatagccacg agccattgtt ttttcaaaat ctttttcata
    50521 aatatctttt gctgataatt tcaatgaaac atctgacatt ttttccacct cattagaaac
    50581 tttgatcttc agaccactgt aagtcattga tgaatgctgt taattcatcg aacgtcacaa
    50641 tatcacaagt atagtttgtt ttatccataa aatcaactaa catctgcttg aagaacttag
    50701 gactcgcctc ttgttcctca tcataaatga gcaatgtttg atccgaatgt tcaagcataa
    50761 attgatctgc ttgtttaaat tgaaaaggac cttgatacga cgtatgaaaa atactatcaa
    50821 cataatctgc atgcttaatt atgttggcat acttactttg attatgttca ttccactttt
    50881 ctgtatgtcc ttggaacggt gtaattacag caaactttaa cgaatcatat gttcgttgta
    50941 attcaataac aacttctgca gtccataatt ctatacccat ttgcccttgt attaacaccc
    51001 attctaatcc ttcatccaac agttgttcaa ttttatgttt tataaattgt tttaaataat
    51061 gtacttcagg tgcgtcatct ttaaaaatgt ttaattcgaa tgatttgtaa cctgttacat
    51121 aaactgtttt aaccataagt accctcttta gttacgccat ctaatatatt ttgtaaatca
    51181 tattgaacac ttttaagctt ttcaataaat aactttcgac ttgttcgctt aaaatgacat
    51241 tcgacagaaa gttgttcaat gttagcaatc aatagctcaa atttccttga attcatataa
    51301 ggtacttcta taataaattc acgatgtaat ttgatctcat tgagcaagct atcaatatgt
    51361 tcagtatatg gctttacagt ttggtaaaaa tcatgatctt gttgttgtga tttcacattt
    51421 tcgaaatttt gttgcatgtt gttaacttca taaattagtg attcgactac atcattcatg
    51481 aggacgcctc ctacattttt taatttatca caatatactg tattcgtcat gttttaacac
    51541 tctatataat ttgatttaac ttattttcaa atgtgttatc tgttaaatca agtaaatcta
    51601 aaaacttcct atataaatac aaaattttat cgtgtatgtt gttatacgat gaaaatactt
    51661 ttaatctaat aaaatcattt aaatcaaata c
//
LOCUS       DAKSQE010000005        45658 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.005, whole genome shotgun sequence.
ACCESSION   DAKSQE010000005 DAKSQE010000000
VERSION     DAKSQE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 45658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45658
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.005"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            288..491
                     /locus_tag="O0V33_000184"
     CDS             288..491
                     /locus_tag="O0V33_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8974878.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            675..1610
                     /locus_tag="O0V33_000185"
     CDS             675..1610
                     /locus_tag="O0V33_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCX8974879.1"
                     /translation="MRYLVNNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPETLDE"
     gene            complement(1691..2659)
                     /locus_tag="O0V33_000186"
     CDS             complement(1691..2659)
                     /locus_tag="O0V33_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCX8974880.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTHYQFKMPVKVDKHYGSTVYTVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVNGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN"
     gene            complement(2780..3136)
                     /locus_tag="O0V33_000187"
     CDS             complement(2780..3136)
                     /locus_tag="O0V33_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCX8974881.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEHEYRK"
     gene            complement(3178..3588)
                     /locus_tag="O0V33_000188"
     CDS             complement(3178..3588)
                     /locus_tag="O0V33_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCX8974882.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     GVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            complement(3968..6034)
                     /locus_tag="O0V33_000189"
     CDS             complement(3968..6034)
                     /locus_tag="O0V33_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCX8974883.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVVLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKIVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGVGQIIPLSEVPDQVFAGK
                     MMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQDADPGK
                     LIMTVK"
     gene            complement(6334..8073)
                     /locus_tag="O0V33_000190"
     CDS             complement(6334..8073)
                     /locus_tag="O0V33_000190"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCX8974884.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELREFIEMAKIPVIHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAMASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLD
                     QIPPISVAAKRFL"
     gene            complement(8113..8802)
                     /locus_tag="O0V33_000191"
     CDS             complement(8113..8802)
                     /locus_tag="O0V33_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCX8974885.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLIILFY"
     gene            complement(8795..9190)
                     /gene="cidA"
                     /locus_tag="O0V33_000192"
     CDS             complement(8795..9190)
                     /gene="cidA"
                     /locus_tag="O0V33_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HCX8974886.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKQRKGIDAYE"
     gene            complement(9404..10282)
                     /locus_tag="O0V33_000193"
     CDS             complement(9404..10282)
                     /locus_tag="O0V33_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCX8974887.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            10449..10667
                     /locus_tag="O0V33_000194"
     CDS             10449..10667
                     /locus_tag="O0V33_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCX8974888.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            11055..11486
                     /locus_tag="O0V33_000195"
     CDS             11055..11486
                     /locus_tag="O0V33_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCX8974889.1"
                     /translation="MKYKKILFRLLIAFAVLFSADFIYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYIY"
     gene            complement(11626..12906)
                     /locus_tag="O0V33_000196"
     CDS             complement(11626..12906)
                     /locus_tag="O0V33_000196"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCX8974890.1"
                     /translation="MQSLDKNFRHLSRQQKLQQLVDKQWLSEEQFNILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHQIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASKDGQYRGIATWRYDQERQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAQVAEALKQEPRANTQVAERILQDLRSQQ"
     gene            13161..14327
                     /locus_tag="O0V33_000197"
     CDS             13161..14327
                     /locus_tag="O0V33_000197"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCX8974891.1"
                     /translation="MTLGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VISHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVDFDEQQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            complement(14498..15019)
                     /locus_tag="O0V33_000198"
     CDS             complement(14498..15019)
                     /locus_tag="O0V33_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCX8974892.1"
                     /translation="MVYKSYYDSPVGRLELVSDGISLTAVLFENQQDDGTREENTSLA
                     IFKEATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQIPYGTLTTYGAIAKKVG
                     KLLDKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYVPKHSTKP"
     gene            15382..17487
                     /gene="clpL"
                     /locus_tag="O0V33_000199"
     CDS             15382..17487
                     /gene="clpL"
                     /locus_tag="O0V33_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HCX8974893.1"
                     /translation="MNNNFFNSDFDSILRRMMQDMQNSNQTSNKKYYINGKEVSPEEL
                     AQFTQQGGNHSAEQSAQAFQQAALRQQGQQSGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISI
                     DISSLEAGTQYRGAFEENIQKLVEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNIMKDAALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFETHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAIDKVT
                     TEKRISELEHDKRKAVSAEEYKKADDIQKEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDANDIERLKNISSRLRSKIIGQDKAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKEALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDTEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDIDVEDNELVVKGK"
     gene            complement(17557..17727)
                     /locus_tag="O0V33_000200"
     CDS             complement(17557..17727)
                     /locus_tag="O0V33_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCX8974894.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDIDRDCCG
                     TEQHTANHFPGK"
     gene            complement(17740..19734)
                     /gene="feoB"
                     /locus_tag="O0V33_000201"
     CDS             complement(17740..19734)
                     /gene="feoB"
                     /locus_tag="O0V33_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCX8974895.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKHLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVAEQVEAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTVLKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV"
     gene            complement(19746..19973)
                     /locus_tag="O0V33_000202"
     CDS             complement(19746..19973)
                     /locus_tag="O0V33_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCX8974896.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(20188..22677)
                     /gene="farE"
                     /locus_tag="O0V33_000203"
     CDS             complement(20188..22677)
                     /gene="farE"
                     /locus_tag="O0V33_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HCX8974897.1"
                     /translation="MAKFLYKMGTFIAKYKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPSITKDIKKTLDDIKK
                     DDKHIDKISDPYENKQINKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAVGTAG
                     SAVIFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVIFAVFSALTLLPALISIFHKHI
                     KVNKLQSNFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYNKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFATVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTFV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVY
                     PQGATNVSKHQDVHGQDDARSIVLDDKTMALYQEVKQQSASSLFLYDALMDYQNKHQL
                     NSKQQATNIEQLNKNIEKLNQLLEKNLRNKS"
     gene            22822..23223
                     /gene="farR"
                     /locus_tag="O0V33_000204"
                     /pseudo
     CDS             22822..23223
                     /gene="farR"
                     /locus_tag="O0V33_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
     gene            23257..24824
                     /locus_tag="O0V33_000205"
     CDS             join(23257..23932,23932..24824)
                     /locus_tag="O0V33_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611580.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSau2 family transposase"
                     /protein_id="HCX8974898.1"
                     /translation="MKTLKGALFMSIKPKVDIDVLLYMFQEYEKGVSFQYLIDTLQLD
                     INVNTLYPKYKYYKTHGIETLLTQKQYNHYSKELKLKVVNEYLNSNQSTQDIAIKYNI
                     RSSTQVKNWIKMYTVGKEIKNQSPKTGVYIMKARKTTFEERVTIVEYYLNHKQSYREV
                     AEHFNISYGQIYQWVHKYQAHGKNGLVDGRGKGKPKSMMTPEEQKEAEIQALKAQNRL
                     LEMENDVFKKVSSIGKRDDSKRKQITAYKTIEALKHQYPIQRLCRILGISRASYYKWV
                     HYQSSELELENEQLKREIESIYHKYNGIYGYRRIYIYIRLKLGKQVNRKRIYRLMKEL
                     NLKAVIRQKRKPYRRSTPQITSENKLNRQFDIDTPNKVWLTDVTEFKIKEGSKIYLSA
                     IYDLGAKRIVSYELGPSNNNQLVFKTFNQAIEKVENTKGILFHSDRGFQYTSKTFKHM
                     LDECGMIQSMSRVSKCIDNGPMEGVWGTIKSEIFRGNKHFKFNSVEEATKTIHDFILF
                     FNHERITLKMADSV"
     gene            24882..25094
                     /locus_tag="O0V33_000206"
                     /pseudo
     CDS             24882..25094
                     /locus_tag="O0V33_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
     gene            complement(25188..26534)
                     /locus_tag="O0V33_000207"
     CDS             complement(25188..26534)
                     /locus_tag="O0V33_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCX8974899.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISDGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAVAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLAVGIVFGNAVKYMGLFGAILFIIIVGATAILLKGRQKPQQ"
     gene            complement(26549..26833)
                     /locus_tag="O0V33_000208"
     CDS             complement(26549..26833)
                     /locus_tag="O0V33_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCX8974900.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            complement(26835..27278)
                     /locus_tag="O0V33_000209"
     CDS             complement(26835..27278)
                     /locus_tag="O0V33_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCX8974901.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTATNAQDIKNILKEHD"
     gene            complement(27283..29238)
                     /locus_tag="O0V33_000210"
     CDS             complement(27283..29238)
                     /locus_tag="O0V33_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCX8974902.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTANTTILNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHIRIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINKQPLSIESAITVAVQPLINDGIVNSNYTAAILKQLEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLCRQDFLDGNIFKIKQHIALTMTKEV"
     gene            29450..29869
                     /gene="mepR"
                     /locus_tag="O0V33_000211"
     CDS             29450..29869
                     /gene="mepR"
                     /locus_tag="O0V33_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HCX8974903.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            29976..31331
                     /gene="mepA"
                     /locus_tag="O0V33_000212"
     CDS             29976..31331
                     /gene="mepA"
                     /locus_tag="O0V33_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HCX8974904.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVALPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMISMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMSNKGRMKDVIKAVIMSIGVIFVVCMIAV
                     FTIGHHMVGLFTTDQAIVEMATFILKVTMTSLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGVVIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            31437..31877
                     /locus_tag="O0V33_000213"
     CDS             31437..31877
                     /locus_tag="O0V33_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HCX8974905.1"
                     /translation="MYKSKILLKYIFSEESDVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKAGYFVTCWTKDENNCNQPYSKEAFADYLMIIVIDEELSGYFLFPRELLV
                     EKGILTTFEHKGKMAFRVYPKWCNQLNKRAEQTQKWQCKYFFEY"
     gene            complement(31961..33319)
                     /gene="glpT"
                     /locus_tag="O0V33_000214"
     CDS             complement(31961..33319)
                     /gene="glpT"
                     /locus_tag="O0V33_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCX8974906.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLVLT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYILIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            33631..34389
                     /locus_tag="O0V33_000215"
                     /pseudo
     CDS             33631..34389
                     /locus_tag="O0V33_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(34612..35148)
                     /locus_tag="O0V33_000216"
     CDS             complement(34612..35148)
                     /locus_tag="O0V33_000216"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCX8974907.1"
                     /translation="MGSKSINNREEFGHYEIDTVWSVRPSTYCLLTIIERKTRYLYAS
                     RLNTRKSNVVCNEIINIMKPLLPKSITMDRGKEFALFEQIEDELNCSIYFSDLGCPYQ
                     RGSIENVNGLLRQYYPKGTDFANITQKSLDEAVKQINTRPRMIFDYKSSEEMLKYHVS
                     TQNCEPILNDCVRHEPVN"
     gene            complement(35129..35632)
                     /locus_tag="O0V33_000217"
     CDS             complement(35129..35632)
                     /locus_tag="O0V33_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCX8974908.1"
                     /translation="MKKRKKRKELDYKTRLAIAKYLNDGLNIIQISKILGRDDAVLYR
                     EVNKRSVDGVYNPLLAEEDAIKSRSISDEQVAKRRRLNDETKAYIEEKLSLKWSPRQI
                     ASQIEKDTGHYISYPTIYRYIRNGLVKVDVKRDMRQAGKKYNKSSEKRGKLDVGARVI
                     KYRLKKY"
     gene            complement(35741..36082)
                     /locus_tag="O0V33_000218"
     CDS             complement(35741..36082)
                     /locus_tag="O0V33_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystatin-like fold lipoprotein"
                     /protein_id="HCX8974909.1"
                     /translation="MRRWFVLILGLVILLSACGQKYDKEIDAVLNSERKSMSESSFKK
                     PEKSNSDFKVYEDGKFITISFVYDKDGTVWTSLYKKNETTDKYVKVEDMNEKEYQSNH
                     KPVYEENNMKK"
     gene            complement(36138..36728)
                     /locus_tag="O0V33_000219"
     CDS             complement(36138..36728)
                     /locus_tag="O0V33_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8974910.1"
                     /translation="MKQKIIMFTLVTVILFCAVLIGYQIPKQQVKMKQNQIEDLQEEQ
                     RILRDKNGELNKLVKRQSKTVISDEEKQIREVSSNFVKQMFEMKKDSSFKSKAPQIKP
                     LVTKDYYDTLFKDSKDKYDLYDDITVNDIHVYFDTYDPKKDSYKVFVQFDERIETDGD
                     DKIEHRQTSAQLDLVRTAEGWRIDNLKRFNLKPLGR"
     gene            complement(36735..37781)
                     /locus_tag="O0V33_000220"
     CDS             complement(36735..37781)
                     /locus_tag="O0V33_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCX8974911.1"
                     /translation="MVNKKDIAKKVIGKTPIGVKLKLAKVIIILCVVAFFTFPVIVMF
                     LLAPDSNKDKDDAGCTVSGGNVSANGIDKFNENAKGGKLEGKGKEIQKIAEKNKVPVN
                     IFMAIIASESQWGKGENATRQNNPLSVMGSKSIHDSTYPTIEDGLNAGAKNLYDVYIA
                     KGLDTPKKIGPKYAPVGASNDPNNMNARWIPTVEKIMKDLGGSEAKTSCSNGKGKSIK
                     FNGKLPHWSNDDPGKGNLYTAGQCTWYAYGMRQKMGKPVSTYWHDAHKWNDRAKAEGY
                     KVDKNPEPGALFIAEQGAGGHDGHYGHVAVVIGVSDGGKTFRITEMNWEGAFKVNERT
                     LKMTDGYSFIHDKE"
     gene            complement(37771..39618)
                     /locus_tag="O0V33_000221"
     CDS             complement(37771..39618)
                     /locus_tag="O0V33_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8974912.1"
                     /translation="MKHKSVYLLCFLILPILLLTSVSAYAVSNPVGEPSHAVKPKIEK
                     YDLSHYRSIYSEKGDWNPFGQEEISRQINNVSDFFFSMTKILAGVTDYAIENLFQLDV
                     INDFADKIGDFVGDIYQKLLSNLAITLFIIVCFNAFIIFSVQGNAREALKRAFLIMCL
                     IGFGVGILANAGNIIRGTNNIGKDLNNIIMNSTSSIDGNIDYIHENSGMNHIRNQLFD
                     MTIYRTYLVMNYGTVDEKEIKAKGKDRIDNILKLDYDKKSEKELDDIVKDEVEKKNNK
                     YMTQGYVFQKLAISVIGFIITLFMSIVFLSISFAKLIFSTFALFLFLFLVFSWIVSFI
                     PGFELSVFSAFAKTLGYIILSACMTFLFVIVGLCIKLANSFIDPDSQNAYFLNSIFII
                     VILFVMYKKRAQIINFVSRGNISFSPSAIGAGVMNRTQERFNKIRHEQAQNKKAKREN
                     QRDEPAPPLQNDNDLRRRQQYKPMPLFINKENQKNGNKRREQQESMNGNDVKSASVES
                     NANNYSKQPQKASQQEHQVRETRQRKDIQRSPQVVNQPLNNENHSINRKEQKSVQTAY
                     DTDVQKRQIQNATQNQQSRQSGNRNQPITRNSQSKDRLKEQKDINKHGK"
     gene            complement(39623..40981)
                     /locus_tag="O0V33_000222"
     CDS             complement(39623..40981)
                     /locus_tag="O0V33_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCX8974913.1"
                     /translation="MAMNEIALFKGVRIQPYQKYIDFMFASVLAFIFLVYRIYILFID
                     YKSVNKKLDILTLFAYFKPHLLYLLIGTAIIFVICKLIAQLLIRFKTKDLAEMIETQG
                     FHNRTVIKKTTEYIPIDYKQTEYDYYPTMFYKRGKKTFVIHIKKDGSRFQDNYLELET
                     IIEPMFNSELVEKKHIGRYLRYEFMPLKYKKRIVMNGEQESNTIFYDTKIAITHQITW
                     DFVKAPHGLVTGITGGGKTYFLFYVIRELFRRHSEVRLLDPKVSDLSFMKRVIGDDKV
                     ADTKGQILKQLREANNEMEERFRLMNDSSDYKIGNDFRNFDMRPYFIIFDEVTAFTST
                     LDKKELQEMNDYLINIIMKGRQAGVFMFLTAQRPDADVIKGNVRDQLGLRVSLGNLSN
                     DGYRMTFGQTDKEFQTIHDSDIGRGYITILGQYNEPILFDAPLMEQYDFVEDVKQILN
                     KE"
     gene            complement(41035..43530)
                     /locus_tag="O0V33_000223"
     CDS             complement(41035..43530)
                     /locus_tag="O0V33_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCX8974914.1"
                     /translation="MNLKAPYAGLRDNLLLTKTGEVWAYYRVRSESISTANYDAKEES
                     KKRMRYFLESIKGYQDFHFEMYDHDLDLRNKFKEISKDFDDEAFNVAEYYSKETIDVL
                     EQELQMITHNSFVMGVKIRELADDAVTLKEILKSTLSDTAERIVSNFGFDVNLDDKIL
                     DKYKTFERDLADSFLGIGGERLTENELAYIIRKPFISNATHDVQEESSRRAITDIYNA
                     VIDPTRLSVLEVENDNEKFYTTTVVVDDFPLDMEYTHLFEKAQNMPFPVECHVKAKII
                     NNEKVKKKFDRAKINHKQQAKEKQDTGDSASEDVQDNLFVLNQMEREVTGSGVFIEWV
                     FMFVIKSDDYRDCKRKAKRLMKRLKETQIYAVNPLADQLQLFYQCLHGNDLFINKYWL
                     QRTTATGIAENLFGVSQSLGTKTGFYIGRIDKFIRSVSREEAVASSRDIILFSLLLAA
                     KGIKGAVSDSPHVLITGQTGKGKSFLAKLLWIYTSFFKGQMLYWDPKSEFAEWFDRVT
                     ESKEMQEKYPLFINHLKTFKYVTLNYEDSTNYGCLDPIVFLDGIEANEMLKSVFDEIK
                     SFENYHHIETAIYQAISDTVEERENGKKVGSLNVIEKLQNNEDEHVKNAGDLLFEKTQ
                     NNILKLVFSDGTNPALNIQEKATILQVKGLDMPKADDDTSSYSTSEKNGITLMLLIGK
                     FLEKFGSRRDVQTTIFIDEGWAFSASRQGKKVGKRIKRTGRSENNSLVFITQSVKDKA
                     DDDGGNFGCHFAFDEKDEREDILKSLGLEYSKESPENMEMLKDLKKGQCIFSDFYGRV
                     GKMVVHCPFEEMTEAFRTQEDSASSKAEEKFAM"
     gene            complement(43565..43948)
                     /locus_tag="O0V33_000224"
     CDS             complement(43565..43948)
                     /locus_tag="O0V33_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCX8974915.1"
                     /translation="MDKKAYNLKRTFEQPIVMYEFTDKFRINKGFRLDFWATFLIVWF
                     ILFLLFWYLLQPVIMSIGGLMFIYFTVAPYYITKYIVKLKQDGKKLFFFLWDFVIFVF
                     NVQLRKVKLSYDEEVEYHDKKITFK"
     gene            complement(43960..44220)
                     /locus_tag="O0V33_000225"
     CDS             complement(43960..44220)
                     /locus_tag="O0V33_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8974916.1"
                     /translation="MSGLFNFFVLGADRPSLGGVGDWISNEVGTGIGLVVLVVGIVKW
                     ASGKYGHMVILFVVGGFLFLVSKGPEQVFNAIAGVWKMIFGG"
     gene            complement(44225..45280)
                     /locus_tag="O0V33_000226"
     CDS             complement(44225..45280)
                     /locus_tag="O0V33_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCX8974917.1"
                     /translation="MKIISKYILTRFSKNGKHFDIPKEQRRVIPKFKNRRKMIVICFY
                     SLLAILLILLLASFIKATRANNDSKEAVNKTNMIQKKYEDNANTVQYSPKLKLYADKF
                     IDTYMNISKDSKELESREKELLKYFPSDYKKPDEKISDTERKLNSKEFYNIKRKDKQT
                     IIQYIVNYDVNITEKKEVKVKKKKKSEKDKDEYETKTEEKQRKVNQNILINIPIKSEN
                     NKYVVVEYPYFTPIPDSQLNKAKMVEDNLKDNKREDNPKAKAFIEDFFNKYASSKSDD
                     MAYLMDNPEGLEGTREVSQIREIRLYPKGDDYVAKVEILMKDKDSPLENLEHYTLDIT
                     KKDGKYYVKNMTNSIGG"
     gene            complement(45341..>45658)
                     /locus_tag="O0V33_000227"
     CDS             complement(45341..>45658)
                     /locus_tag="O0V33_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCX8974918.1"
                     /translation="KNRKRWPLYRPWALFIKDTEKLNLTTRPTLKSIEDNLDWLCKQV
                     ATTLDTVLTAESMAQSEGLLTDTDFLDKILAHSQFNDEHTNRINHYLEALKQKKHLSK
                     DKC"
BASE COUNT        15015 a         8292 c         6797 g        15554 t
ORIGIN      
        1 atttctaaaa acttttaata tttatacatt ttatctttcg agctatttat ttcttatttt
       61 tgtgctgtgt ttataaaacc gcacactata ttcaaaaata acttattaca catatatttg
      121 caataagata attaaagtag gatattattt ttagttttct gataggaatg atgatagtct
      181 ataggttgaa tctttacttt ttttaaagct aaatttacat caacttaaca atggttggtt
      241 atactgaaga tgaaaatatt tagtataact tagtggaggc gataaaggtg caattgagtc
      301 attccgttaa agttgcaatt tctatctatt tagcacttat ctttataacg ttcacttctt
      361 atttagtcat tattttatat acgagtatga ctggacatga tgtatcacat ttcgttttag
      421 atagtcagca ttctcatcat ggatctctta cgcaaaaaca tttgagtctt cctgaaatct
      481 catttaaata gtttatcctc tgtgtttcaa cattcatttc ccatatcgat tcatttatct
      541 atcatccaga ccactacatc ttagatgatt ttttttattt tctcatttca ctctttctta
      601 aaagtcgata taatgaatta aatcattatc atacactgac atattttatg ttgttggtgt
      661 taagttttaa aggggtgaga tacttggtga ataatcattc agctttgcgt ttaaaaggaa
      721 ttatacttgc cattgtcggt gcttgtttat ggggattagg tggtactgtt tctgatttct
      781 tgttcaaata taagaatatt aatgtcgatt ggtacgtcac tgctcgactt gtagtcagtg
      841 gtgttttctt acttattatg tacaaaatga tgcaacccaa acgttctata tttagcgtat
      901 tccaagatcg acgtatgtta ggcaaattac ttatcttcag tatactgggc atgttagttg
      961 tacaatatgc ttatatggca tctattaata caggtaatgc tgcgattgca acattactac
     1021 aatacattgc gccagtctat attattattt ggtttgtcat aagaggcgtt gcaaaactaa
     1081 cattatttga tgtgcttgct attatcatga cactattagg aacattttta ttattaacga
     1141 atggttcatt ttctaattta gtcgtcaatc ctgcaagttt attctggggt attttagctg
     1201 gtgtagcact cgctttttac acaatttatc cttcagacct acttaaccgc ttcggttcga
     1261 ttctaattgt cgggtgggca atgcttattt ctggtgttgc gatgaattta cgccatccaa
     1321 tttggcacat tgatatcact aaatgggaca tatcaattat attattttta atctttggta
     1381 ttatcggtgg taccgcactc gcattttatt tctttatcga cagtttacaa tacatatcag
     1441 cgaaagaaac aacattattc ggaactgttg aacctgtcgt agccgttatc gcaagcagtc
     1501 tatggttaca tgtggcattc aaaccatttc aaatcgtagg catcattctt attatgattt
     1561 taattttatt actatccctt aaaagacaac ctgaaacatt agatgaataa gaaaactctg
     1621 ataatcactt tagcaagtaa ctatttttaa caacgtagtt accttatagg tgatatcaga
     1681 gttttttatt ttagttaata atatttttca cttggtataa aaagcgtcgt cgctctggta
     1741 tcggaaatac tggataaata tggaattggt aatatccagg tataaagtca tgttcgatac
     1801 cttttgcact caacaattgc gataagttca aagcatctgg gtacagtact tcttttgtgc
     1861 caacagttaa tgtaatacga cctaaaccat ctaagtcacc attaattggc gatactttgt
     1921 agttatctaa aggtgtgtcc cctgcccatt gttcagctaa gaacacactg ccattcacac
     1981 ctaccattgg gtctttcttt aagtagtcag gaatttcagg atgctgcatc gttgcatcta
     2041 aaactggtga aattaataca atatgtcctg gttgcacaat atgtttttct tttaacaatt
     2101 gagcaaatga tagagcaatt tgaccgcccg cagagtcacc cattacaacg atttgtttag
     2161 aatctgctac ttgattcaat aaatcatggt acaacttttc aaaaagcaca tacgtcgctt
     2221 gataatcttg atgcggaatc ttcggatata ctggcatgat gactttagca ttgagtgttt
     2281 ctgcaagttc atcaataaat tcgaaatgaa ttttgagtgg gtcttggaac catgcgcctc
     2341 catgtgcata taatacaacg cgttgatgct tatcttgttt atcgttaacg gtataaacgg
     2401 ttgaaccgta gtgcttatcc acttttactg gcatcttaaa ctgataatgg gtgccctcat
     2461 attcacctgc tgtctgtggc gccatttttt ctaatgcttt catcgcttct tctttataca
     2521 taaaacgacg cttagctcta gataatcgta taccttgttc taacatataa cttttcacac
     2581 tgcgtttttc tttaattcta atatgtgcgt atgccgctac taaaaatcca aacgcaagtg
     2641 tagaccattt tttacgcatt tgtcgtcact cctctttgat gatttctact atagtattaa
     2701 tgtaccatgc tgattcttat tactaccaat atttactggg aaataagtta taatgtctac
     2761 ataatcttag caatactaat catttgcgat attcatgctc atcgacatcg ttattcacac
     2821 tatcattcat ttgcttaaga tgatgttcaa aacgttgcat atcgataaaa cgcccttgac
     2881 gatgcatttc ttttccattc ataaaaatta aatccacagg tacagtgaaa atatttaatt
     2941 ctccagcaat agcttccacc tgactttgat taatcacagc taatggcaca ttggggtatg
     3001 attgcatcaa gtcttcaatt tgtggtaaaa cggcatgaca cacgctacac tgatcgcgca
     3061 tgacatgtac aactgctaac ggatattgat gaataaattc acggaatgct tcgacactat
     3121 ttaattgctt aaccatttcg aacaccttct tactttttat attttaaaat gaagtcatta
     3181 ttttgttgct aaaatgtctt gaaatacttg tggcacagag gtaatcttct tatcgctcaa
     3241 tctgtaaaaa ccgcctgtct gtgtaccaat acaatgcacg ccatgtgcgg acacaatttc
     3301 aaactgaaac acccatttca tcttgcttgc tttaactaag taaatacggc ctactggttt
     3361 gtcatgtatt gtgacaggta ctttatattc tgcttccgtt ttcataagta taggtgaaat
     3421 gccttgattg atcattgttg agtaattcat gtgctgatta ataaacgctg ttctcaaatc
     3481 ttcgaaccat ctaatataaa cgatattact aacgacaccc atcgcatcaa tgtcgtaacc
     3541 attcacttcg atttctttct ctgcaactaa tagaccttct gttgtcataa tgacaaatct
     3601 ccttatctta caaatagatt taaaatcatg ttaatttatt tttattactt tcatgataaa
     3661 aatacaagca attgaacttt attacggaaa atcaaacttt tcaatgacgc tattaatttg
     3721 gctagtcgcg aagatgcatt tttctaaaat gtgacaaaaa cataagcaat gttttctata
     3781 cctaacacta atatttaact tattctcatc aacacacctg ctatacatca ttgcgcacta
     3841 aaaagacagt agcttcattt taagctactg ccttttgtaa cgtattctct ttcaactcgc
     3901 tacttcgcat acgcactatg aatgactaaa agttcattca tttgatatgc tatttcattt
     3961 tcaatcatta tttgactgtc ataattagct tacctggatc agcatcttgg acatcttcaa
     4021 tgacaagttc tttattttca agatttgtaa taatcattgg tgtaacgatg cttggtgcgt
     4081 gcgctttcaa gtatgctaaa ttcactttca ttaatggttg accttgtgtt actttttcat
     4141 caacgttaat cagactttcg aatccttcac cattcagttt cactgtatcg ataccaatat
     4201 gaataagtac ttcgacacca ctttcagatt ctaatcctat cgcatgtttc gtagggaaga
     4261 ttgttttcac tgtaccatca aacggtgcta caatttcacc tttttcaggg ataaagccaa
     4321 caccatcacc catcatttta ccagcgaata cttgatcagg tacttctgat aatggaatga
     4381 tttgaccgac acctggtgca tagatgtctg ttgttccaag tgcttctacg tgaactggtt
     4441 catctgacat ttcttcagtc actgtcgttt cacttggctt tgtaatttca ccactcataa
     4501 tcttcgccat atcatgttta atttgatctg attttggacc aaagatagct tgcatattgt
     4561 ttccaacttc taatacacct gatgcgccta aagctttaat acctgctaca tctacttttg
     4621 atttatcaac cacttctacg cgcagacgtg taatacatgc atctaaatgt ttaatgtttt
     4681 cttttccacc cattgcatct aagacatcaa atggtaattt tgcgacacta gagttacgaa
     4741 tttcagtttc ttcatcttca cgacctggtg ttttcaattt aaacttacga attgcaaagt
     4801 cgaataagaa gtaatacacg atagcatata caataccgac tggaataact aataatgcgt
     4861 gtgaacgatc ccagtttaat aaaccatata aaatataatc tatgaaacca cctgagaatg
     4921 tcataccaat tttaacgcct aataaatgca ttactaagaa tgatgtacca gctaataata
     4981 cgtgaattcc ataaagtact ggtgctacaa ataagaatga aaattctaat ggctcagtga
     5041 taccagttaa aaatgcagtt aatcctgctg ataacattaa accacccacg atttttttac
     5101 gttctggtcg tgcattttta taaatagcaa atgccgccgc tggtaaacca aacatcatga
     5161 atggatattt accagtagta aatgcaccag cagtaaatgg tacgccatct ttcaattgtg
     5221 ccatccaaat acgttggtca ccacgaacta attcacctgc gtgatttgta taacttccga
     5281 attcaaacca gaacggtgaa tagaaaatat gatgtaaacc aaatggaatt aatgagcgtt
     5341 caataatacc gaatataaaa gttgttaatg ttaaattttt atttaataag aaattcgata
     5401 aactatttaa tccatcttga attggtggcc acgcaaaact taaaaccaca cctgttgcaa
     5461 ttgctacgac cgatgtcaca atcggtacaa atcgtttacc tgcaaagaat cctaaaaatg
     5521 gtggtagtgt aatattataa aatttgttgt aacaccatgc ggctaaagca cccatgataa
     5581 taccaccaaa cacacccgtt tgtaatgttg gaatacctaa tactaaggca tgtgctggtt
     5641 ctttcgctgc ttgacttaat tcttttgccc ctttggcata tgagaaaatg tcatcaattg
     5701 taatatgcaa cactttcccc attgttgcat tcataattaa gtaacctact agcgctgcta
     5761 atgctgcaac accgtctcct cctgctaatc caagtgctgt accaactgca aataataatg
     5821 gcaagttatc aaatacaact tgtcctgctg cttccatgac cgacgaaatc attacaatga
     5881 tatcgttttt taaccatggt gcaatttcta ctaattgttc gttgtgcatt gcgttaccaa
     5941 acgctaataa aataccagct gctggtaaaa tcgcaacagg taacattaat gctttaccga
     6001 tacgttgcaa ttgtccaaac aatttcttaa acatataaac atcccctatc tcaatttccg
     6061 cttccatgta caaaaaagca ggcatgaaca aacaagacta ttctcatagt acggttgttt
     6121 gctcatgcct gcattaccag taacacgcaa gtgatatgta attcatagct attatacata
     6181 tttgtaaaag cgttttcaac aactttttca caattttatc atcaattcag tacaatcatt
     6241 tgtgataaat aaacaaaacg gcaattccca ttttgttgaa atagaaatta ccgtttttat
     6301 agttcaattc aattgtgata acctttcaat cagttataag aaacgttttg ctgcaactga
     6361 aataggtgga atttgatcta aatctaaatg tttatcttca gtaatagatc taaatgccca
     6421 tttaccataa ccaagcgctt cttcatttac aattttacct ggtaatggcg cagcattagg
     6481 atcaacataa acatctacaa ttgttggtac atcttgtgct agtgcctctt ctacaatagc
     6541 atctacttcg ctagcacttt taattgtata acctttacca cctgctgctt cagcaaattt
     6601 ggcatgatcc atatcagaga aatcaaccgc atattctaat tcaccagcag cttgttgttc
     6661 atatttaata aacgctaatt gcttattatt tagaacaaac actgttaaag gtagatcata
     6721 ttgtacagct gttgcaaagt cctgcatcac catttggaat gcaccgtcac cagcaattgc
     6781 gatggcttgt ctatttggat atgcaatttt tgatgccatc gcacctggaa gaccgcaacc
     6841 cattgtacct aaccaacttg aaatgatgaa cttgttattt acaccaaggt ttaagtatcg
     6901 agttgaccaa actgttgctg tacctacatc tgctgaaatc actgcatcat ctttaataaa
     6961 tttattgatt gatgccatta atcgttctgg acgtaatggt ttactattat tatttttatc
     7021 ttgttccatc catttatccc aaaccgcttt acgttctaac gttttgttta agaatggtct
     7081 ttcagcaaca tgtttaatat tttcagttaa ctgatgcaac gcaattttac tatctccaac
     7141 aattcctaca ttaatattga aacgatgtcc gatattttta ggatttgtgt caatttgaat
     7201 ggctttaata tttttcttag gtaagtaatc cacatatgga tagtttgtac caaccataat
     7261 taataaatcc gcttcctgca ttgtttgata agatgtttta gtaccgattt tacctaagtt
     7321 accgatacta tatggatgat catccggcaa gattgtttta gctggtaatg aatgaatgac
     7381 aggaatttta gccatttcaa taaattcacg tagctcatct ttcgcatgtt tcgcaccaac
     7441 accgattaac atgacaggct ttttactttt attaattaat ttaaccgctt ttttgatgtc
     7501 tttatatttt ggtgatacta ctgttggtct tgatgtatct actggtttat tcgttgtatc
     7561 tttaattttt tcagttaata agtcattagg acaaataaca acagctacac ctttttgttc
     7621 atatgccgta cgaattgctt cgttaacgat ttcaaacaca ttgtcacctt tttcaatttg
     7681 gtgattataa acggctacat cttcacataa tttttgtaaa tttgtttctt ggaatgcttt
     7741 cgttccaagt gctgtactat tcgtttgtcc agataatatt aattgcggta cattatccat
     7801 tttggcatcg tacataccat ttaataaatg aattaaacca gggccaccga tacttaatgc
     7861 cacaccgatt ttaccagtta attttgtata accagcagct gctaaacttg ctacttcttc
     7921 atgacgtaca tgataaaatt taaattgatc tctcactgta cgtaaactat cgaccactgc
     7981 gtcgattgaa tcacctggaa taccatataa gtgatctata tcccatgctt gtaatgcttt
     8041 aactaatgct tcatttgctt ttatttttgc cattattact aatagcctcc ctttcttagt
     8101 gtctttttct aattaataga acagtatgat tagaacaggt attaacaccg aactaataac
     8161 tgcagttaaa atcatcccaa ttgaactaaa tgcaccagat tcaatatcca tctccagtgc
     8221 tttagctgtg ccaaatgcat gtgacgcatt cccatacgtt aaacctttgg cgatagaaga
     8281 ttcaaatctt ccaaatcgta ataacatcga acctaaaata ctaccgatta aacccgttgt
     8341 gataataaaa agtacggtca tcgtatctgt gccaccaagt tcatgtgaca cttctatacc
     8401 aacagcggct gttatagatc ggggcaataa tgttacaatg acgtctttag aatagccaaa
     8461 tgctttaagt gttaagaaca ctaacatgaa atttagcatg acgccagtta atacacttgc
     8521 aaaaatgata gagacattat ctttaatttt ctctctattt ttataaagtg ggtacgctaa
     8581 acatacgacc gttgcgttta aaatatggtt gatccaactg ccacctttca tatacccgtt
     8641 ataactaatt ccaaagataa gtaagacaaa aataattcct aaagatgcaa ttaatgctgg
     8701 attcaaaaat ggatttggat atttttgttg taatctttta gcgaaataat ataaaacgac
     8761 agtcaataaa atcattaaca aggcttgcac gtaatcattc ataagcatct atacctttac
     8821 gttgcttatg tttaacagac attttttcag caatataacc tgaagataat gcaacgatac
     8881 atgttcctat gataatgact gcgaaaaaga gtatataatt tagcgtaatt tcggaagcaa
     8941 catccataat acctacaact gacggtatga agaaaaagac catcgtcttt aataaaaagt
     9001 ttgcaccgtc ttctacccaa gttagcggta caatcttaaa ttgtaatagt aaataaaata
     9061 aaaatagacc aacaatactg ccggctaagg gaagatgaaa aatcttttga atttctgtgc
     9121 caatataagt aatcacaatg atgattccta gttgtagtaa taatttgatt attaattgga
     9181 ctttgtgcat ggcgccatcc ctttctaaat atgtctaaat tgttacaata actattataa
     9241 agatggcgta cacaatataa aaatactctt tttttatggt ttgtattact attttctatg
     9301 gaaacgctct ctaaatttca cttcattttc acaaaaaaaa cactcattat ttcaatgatc
     9361 caagtaatga ggaatcacaa ataatgagtg ctttattaat atgttagcct aaacgatctt
     9421 tcaaaaattc tatccatttc gttgtggcat gacttaattg tttatccttc ttccaaacaa
     9481 cacctaattc ccaatgtaca tgcgcatctt caatgcgtag tagcttcaca tctccattta
     9541 gttgctctga aattgatgtt ggtaaaatac taatacctaa ttcattcgta actaaatctt
     9601 ctataacatg ccattgtgaa atttgcgcta cagtattcgg aacaaagcca acgtttttag
     9661 cattttcaat aattttatca ttcaagtaaa agtctttatt aaataaaatg aagtcttcac
     9721 ctgctaaatc ttcgagttta acagtttcat attttgcgag tcgatgctct ctgctcacga
     9781 taagtcgcaa atcttcctta tctagggtag tataatcgaa aatatgatga tccactggca
     9841 aagtggtcac gcctatatct acttcatcat tgataatttg ctgctcaatc gtttttccgc
     9901 cattttctat taaattatat gtaacatttg gatattgttg atggaatgca ccaagaatat
     9961 ttataagaat cttcatattc atgactgccg acatgcccat gtttatatgc cctgtttcca
    10021 gtccattcaa gcgttccatt tcagatggta aataatcgta tagtgcaaca atttctttac
    10081 ttttctcata aaaaatttga cctgcatcgg taagtattaa atgtctttta cttctatcaa
    10141 ataagggcgt acccatttca ttttcaatat ctttaattgc tttactaatt gtaggttgtg
    10201 caatatataa tgatttggaa gcattagtca tgcctccttg ctttacgact tcaataaaat
    10261 atttcatatg tttgatatcc actaacatca tcctaactat ttcaacacgt ttatttcatt
    10321 atacacaact tcttaaacat tcaataaatt tagatttata aaattgcttt ataaaaagtt
    10381 ttatgtaaaa tacacattct tttatggtat tatattaatg gctcaagttt gttattcgag
    10441 gtgaggtatt gagcttttac gaatttatgc aaagttttct tggtgatgac acaccattag
    10501 gtgaattggt tgattggatt aatcaagata gcaatttccc tagagaagtg aagagccata
    10561 gtgaaatatt gtcatatttt cgaacaaatc catgtcctga aagcatctca gtacctgtaa
    10621 ttaaacgggc actatcagtt ttcaaccaat tcacaaatgt acaatgatac atgaatacat
    10681 ggagtcacat catctcatgt tagcgctctg attgcagggc gctgttttta atgttatcgc
    10741 ttcatttata tgttgtgatt gctattttct ttcagttata gaaccacgta ccttttcttt
    10801 taaatcattt tatattttcc cactaatatc cgctgttaat caatcctgac atccttgtat
    10861 cactatgaca cttaattgtt agatacataa atttctacat tttatgaaaa aatccatttt
    10921 tattacaatt taacacttta tatgacagct tcattacagt tacttttatt gttgattgct
    10981 tgcattgttt tctaaaaaaa ttttgctatc ataaataacg ttgaataaag aaaaaattaa
    11041 cttgggagat aaaaatgaaa tataaaaaga tactttttcg tttactaatt gcttttgcag
    11101 tccttttctc agcagatttc atttatcaat ccgtcgaaca aacgcatcaa tcgcatgccg
    11161 cagttaatta ttatagtaaa aaccaatgta catggtgggc atttaaacgt cgcgcacaag
    11221 tcggtaaacc tgtttctaat agatggggca atgccaaaaa ttggtattac aatgcacgta
    11281 aatcaaaata tgcgactggt cgtacaccaa gaaaatttgc tgtcatgcaa tcaactgcag
    11341 gatattatgg acatgtcgca gttgttgaac aagtatataa aaacggtagt attaaagttt
    11401 cagaatacaa cttttatcgc ccattaaaat acaatacacg tgtactaagc aaaaaggcag
    11461 cacgtaactt taactatatt tactaatcaa aaaacttcta tcacgaacgc ttcaaattcc
    11521 tgtatgcgtg tgatagaagt tttttatttt ttgttcatat taatttattt aagcgctacg
    11581 atggactgac ttaaactttt ctttaaaatc gctattcgca tttgattatt gttggcttct
    11641 taaatcttgt aaaatgcgtt cagcaacttg tgtattcgca cgaggttctt gcttcaatgc
    11701 ttcagctact tgcgcaattt catcaccttt tgcacctaca acaatagcta aagatttata
    11761 ttgcaagctc atatggcctt gctggatacc ttcagaaacg agcgcgcgac atgctgcaaa
    11821 gttttgtgct aaaccaacgg cagcaactac atgacctaat tcttgcgctg actctacatt
    11881 tagtagctct aatgatgctt tagctattgg taatactttt gtaccaccgc caacgattgc
    11941 caatgtcata ggcacctcta tggtaccaat taaacgttga cgttcttgat cgtatctcca
    12001 tgttgcaata ccacgatatt gaccatcttt actcgcgtat gcatgcgcac ttgcttctgc
    12061 accacgcgta tcatttcctg ttgctaaaac aacggcatgt atgccattca taacaccttt
    12121 attatgtgtt gcagcacgat gaatatcaac ttgtgccaat acagaagcac gttccattcg
    12181 tttggcaacc tcttctccag ttctctcgcc ccttgctaaa tctttaacat caatttcgcc
    12241 ttgaacttta acaacggacg ctgttgcatg attggataaa atactcatta aaatgtcgct
    12301 ttgcggaaat tcatttttta aaaatgcagt tatagcctct aaaatcgtat taagcatatt
    12361 agcgcccata gcatctttcg tatcaacaaa tacttttaaa gatagtaact gttgctcagg
    12421 aaatgtatca atagctatac gttggtaacc accaccacgc gctttaatag aaggatatgc
    12481 ctcatccgca atttgatgaa tttgcttctc taaagcttta atgtctgctg ataatttttc
    12541 agtatcgtca acgccatcaa agacgatttg acctatcata atacgttcag aagataccgt
    12601 tttaaatcca ccagtctgat tcactagctt tgcaccataa ctagctgcag cgacaactga
    12661 aggctcttcc accatcatag gtacaacata tgctttatcg tccacaatga tattcggtaa
    12721 taatccaacg ggtaatgcac cttgcgcgat gacattttca attaaactat ttgctacttc
    12781 ctcatcaatt aatggatgat tcaataaaat gttgaattgc tcttctgata accattgctt
    12841 atctaccaat tgttgtaact tttgttgacg agataaatgt cggaaattct tatctaaact
    12901 ttgcatggac atactccttt tacttcacat aattttttaa catttcaatc actactattt
    12961 ttaccataaa ataacgttat tcgtcttaaa attcaattga ataattgtcg ttttgacttt
    13021 aaaataaagc aaggtaaatt aaaacgctta caagaaacgt caaatcattt ttaaatttag
    13081 tgtatttctt tgtataagat tagcatattc tgatatgata caagtgttgc ttttataaat
    13141 ttgaaaggat gtaaaacctt atgacattag gtatcgacaa aataaacttt tacgttccaa
    13201 agtactatgt agacatggct aaattagcag aagcacgcca agtagaccca aacaaatttt
    13261 taattggaat aggtcaaact gaaatggctg ttagtcctgt aaatcaagac atcgtttcaa
    13321 tgggcgctaa cgctgctaag gacattataa cagacgaaga taaaaagaaa attggtatgg
    13381 taattgtggc aactgaatca gcagttgatg ctgctaaagc agccgctgtt caaattcaca
    13441 acttattagg tattcaacct tttgcacgtt gctttgaaat gaaagaagct tgttatgctg
    13501 caacaccagc aattcaatta gctaaagatt atttagcaac tagaccgaat gaaaaagtat
    13561 tagttattgc tacagataca gcacgttatg gattgaattc aggcggcgag ccaacacaag
    13621 gtgctggcgc agttgcgatg gttatttcac ataatccaag cattttggca ttaaatgaag
    13681 atgctgttgc ttacactgaa gacgtttatg atttctggcg tccaactgga cataaatatc
    13741 cattagttga tggtgcatta tctaaagatg cttatatccg ctcattccaa caaagctgga
    13801 atgaatacgc aaaacgtcaa ggtaagtcac tagctgactt cgcatcacta tgcttccatg
    13861 ttccatttac aaaaatgggt aaaaaggcat tagagtcaat cattgataac gctgatgaaa
    13921 caactcaaga gcgtttacgt tcaggatatg aagatgctgt agattataac cgttatgtcg
    13981 gtaatattta tactggatca ttatatttaa gcctaatatc attacttgaa aatcgtgatt
    14041 tacaagctgg tgaaacaatt ggtttattca gttatggctc aggttcagtt ggtgaatttt
    14101 atagtgcgac attagttgaa ggctacaaag atcatttaga tcaggctgcg cacaaagcat
    14161 tattaaataa ccgtactgaa gtatctgttg atgcatatga aacattcttc aaacgttttg
    14221 atgacgtaga ctttgacgaa caacaagatg ctgttcatga agatcgtcat attttctact
    14281 tatcaaatat tgaaaataac gttcgcgaat atcacagacc agagtaatct gtgtatttga
    14341 aagacattta aatataacct aaaaacagca gtaagacagc ttctcattga aatcgtctta
    14401 ctgctgtttt ttatttataa tgcttcgtct tgaatgaatt cataatgcct atttgacaca
    14461 ttattaaagt tttcctattg cctgaatcct ttatacgtta cggcttcgtg ctatgttttg
    14521 gtacataaag ctttgacata tcgatattct ccaactctaa cagcttaatt ttattattaa
    14581 tcgttccacc gaatcctgtt aagctacccg ttttaccgac aacgcgatga catggcacga
    14641 taatagataa tggattactt ccgactgcac ctccaaccgc ttgggctgac atttttggct
    14701 tatcaagcag cttgcctact tttttggcaa tagcaccata cgttgttaac gtcccataag
    14761 gaatctgtct taattcattc caaacacact gttgaaaatg actacctgtt ggctttaaag
    14821 gtattgtgat ttcaggattg tcacctttaa aatacgcgtc taaccactgt gtcgcctctt
    14881 taaatatcgc taaagacgta ttttcttccc tagtaccatc atcttgttga ttttcaaaca
    14941 aaacagcggt cagacttatc ccatcactca caagttccaa tcgtcctaca ggagaatcat
    15001 agtaactctt atataccata aacacacact cctttttcat catcatacta ttcatttaac
    15061 tttatgaaaa tgattaacac tttatgcttt atcaagatca actattcact tccaaatcaa
    15121 aattcacata gactaccaac acaatatacc atttcttaat taactatgta tcaacacata
    15181 cgtttcttaa ccattgatta atatttactt ttaaattctt acttacttca aaaattacac
    15241 aaaaagttca taaagtgcct acaatgcgcc gtttttaaaa ctacaatggt ggaaaaatag
    15301 aattgccatt gtttatagtt tgcatatagt ttcaatatat tttcttttac ttaatagttt
    15361 aaaattttag aggtgatgtt tatgaataac aattttttca atagtgactt cgattctatt
    15421 ttacgaagaa tgatgcaaga catgcaaaac tcaaatcaga ctagtaacaa gaaatactat
    15481 attaatggta aagaagtttc accggaagaa ttagcacaat ttacgcaaca aggtggtaat
    15541 cattctgccg aacaaagtgc gcaagctttt caacaagcag cactaagaca acaaggtcaa
    15601 caaagtggca atggcaatta tttagaacaa attggtcgta acttaacgca agaggctcgt
    15661 gacggattat tagatccagt catcggtcgt gataaagaaa tccaagaaac tgcagaagtc
    15721 ttaagtagac gcactaaaaa caatcctatc ctagtcggag aagctggagt tggtaaaact
    15781 gcgattgtcg aaggtttagc gcaagcaatc gttgaaggga acgtacctgc agcaatcaaa
    15841 gataaagaaa ttatatctat agatatttca tcgcttgaag ctggaacgca atatcgtggc
    15901 gcctttgaag aaaacatcca aaagttagtt gaaggcgtta agtcatcaca aaatgctgtg
    15961 ctcttctttg acgaaattca ccaaatcatc ggttccggtg ccacaggaag tgattcaggt
    16021 agcaaagggt tatctgatat tttaaaacca gcattaagtc gtggtgaaat ttcaattata
    16081 ggtgcaacga ctcaagatga atatcgcaat aatatcatga aagacgctgc attaacacgt
    16141 cgttttaacg aggtactggt taacgaacca agcgcaaaag atacggttga aattttaaaa
    16201 ggaattcgcg aaaaatttga aacacaccat caagtcaaat tacctgatga cgtattaaag
    16261 gcatgtgtag acttatccat tcaatatatt ccacaacgcc tattacctga caaagcaatc
    16321 gatgtattag atattacagc tgcacattta tctgcacaaa gtcctgctat tgataaagtc
    16381 acgactgaaa aacgtatttc tgaattggaa catgataagc gtaaagctgt aagtgctgaa
    16441 gaatataaaa aagcagatga cattcaaaaa gaaattaaat ctctacaaga taaattagaa
    16501 aacagtaatg gtgaacatac tgcagtagca acggtgcatg atatttctga taccattcaa
    16561 cgattaactg gtattcctgt atctcaaatg gatgccaatg acattgaacg tttaaaaaat
    16621 atttctagtc gtttaagaag taaaatcatc ggtcaagata aagctgtaga aatggtttct
    16681 cgtgccattc gtcgtaatcg agctggtttt gatgatggta atcgtccaat tggtagcttc
    16741 ctatttgtcg gtcctactgg tgtgggtaaa acagagcttg ccaaacagtt agccattgat
    16801 ttatttggta ataaagaggc actgattcga cttgatatga gtgaatatag tgatactact
    16861 gctgtttcta aaatgattgg tacaactgct ggttatgtcg gttatgacga taattcaaat
    16921 acattaactg aaaaagtacg acgcaatcca tattcagtca ttctatttga tgaaattgaa
    16981 aaggctaatc cacaaatatt aacattatta cttcaagtga tggatgatgg taatttgact
    17041 gatggtcaag gtaatgtcat caactttaaa aatacaatta ttatttgtac atcaaatgct
    17101 ggctttggca atggcaatga cactgaagaa aaagatatta tgcatgaaat gaaaaaattc
    17161 ttccgccctg aattccttaa ccgcttcaac ggcatcgttg aattcttaca tttagataaa
    17221 gatgcattac aagatatcgt caacttgtta ttagacgatg tacaagttac attagataaa
    17281 aaaggtatta cgatggacgt ttcacaagat gcgaaagatt ggttaattga agaaggttat
    17341 gatgaagaat taggcgcacg cccattaaga cgtattgttg aacaacaagt gcgtgacaaa
    17401 ataacagatt attatttaga tcatacagac gttaaacatg tggatataga tgttgaggat
    17461 aacgaattag tcgtaaaagg taaataacga caatttaaca tatagcgtat caaaaaatga
    17521 gcatcaggac gccctcgcct gtgctcattt tttaaattat ttcccaggaa aatgattcgc
    17581 tgtgtgttgt tctgttccac aacaatcacg atcaatgtca catgtaccac attttccttg
    17641 tttcgaacgt ttgaaaaatt tcactagtgt atacaaggca tatccgaaaa ttgctaaaaa
    17701 aattaaaatg ttaataatga ctgacacttt aaccactcct taaacaaata aatgtccgac
    17761 ttgataaaaa atgaatgtta agacatatgc agtgactaga ggataggcaa ctgcaagtgc
    17821 cgtccatttc catgaataag tctctttacg gattgctgcc actgtagaaa cacaaggaat
    17881 atacaatagt ataaatatca taaatgcata cgcagatagc ggtgtgaatt gattttgaat
    17941 cacattaaca aggcctgcat cacctgatga atagataatc gccatcgaac ttacgataac
    18001 ttcttttgct aaaaatcctg gcactaacgt agcacctgct tgccatgttc caaatccgag
    18061 cggttgcact aacataccaa agaagctacc aaccatatgc aaaaaacttt gattgatatt
    18121 cacattgata ccatgtggtc ctacataact taatagccaa atgactactg agccgccaaa
    18181 aataaatgta cctgctttac gaacaaagcc cttagccttt tcccaagtac tacgccacaa
    18241 cgttttaatg gaagggacac ggtatgttgg caactccaca ataaagattg cattatcatt
    18301 ttttaaaact gtcttagtaa gtactgtact gactaaaaat gccataataa tacctaaaac
    18361 atacaagctt aatactacta aagattgatt ttctttgaaa aagataccta cgaacaatgc
    18421 atacactggc agtctagcag agcatgacat gaatggtgca attaatatcg ttgttaaacg
    18481 ctctttttca ttttcaatac tgcgcgcagc cataatactc ggtacgttac aaccaaatcc
    18541 gataatcatc ggtataaaag acttcccgct taaaccgaac gattccataa tacgatccat
    18601 taaaaccgca atacgtgcca tataacctga atcttctaat aaagatataa agaaaaagag
    18661 cacaacaatt tgtggtacaa agactaatac tgatcctaca ccagcaataa tgccatctgt
    18721 aattaaatct tgtaaaaatg gtataacgcc aagataattc ataatcgtct tcacactatc
    18781 cgtaaatgta ccacctataa atgcatcgag ttgatccgac aaaggtgtgc caatccatgt
    18841 aaatgtagtt tgaaagatca accacataat cgctagaaaa ataggcatcc ctatatattt
    18901 atgtgttaat atcttgtcta ttctagagct gaaatattgc ttatcttcat ctggatacgt
    18961 taccacgtct tgcaataacg tctcaatata atgattgcgt atacgttcca tctctcgacg
    19021 aacagataca gcctctactt gttcagcaac ttgatcacgt aaactcgaca atttatttac
    19081 aacctctgaa ttaagttcat ttgcaatttg catgttattt agtaagaatt gaatcgcaat
    19141 aaatctagct tgatatttat catgagatgt ctctgtcatt attatttgac acatattttt
    19201 aattgtctct tcaatcttct caccataatt gattttaaaa tgcggttgat acccttcccc
    19261 tagatgcttt atttcgccaa gtaaatgttt agttcctttg cctgtacgtg ccacaactgg
    19321 aaaaataggt gtttttaact ttttcatcaa tttatgataa tcgattttta tcccgcgctt
    19381 tgtagctaca tcaatcatat ttaatccgat gtatattggt tgattaagtt ctaacaattg
    19441 tactgttaat tgcatatttc tttttagttg actcgcatca acaatgttaa tgattcctga
    19501 aaaagaatcg tttaataaat agtctgtcac tacagtttca tctttagaaa tcggcgataa
    19561 atcatatgta cctggtaaat caattaattg tcctacattt tctttaagct tccctacttt
    19621 tttctctacc gttacgccac tccagttgcc tatatattca tacgaaccag ttaaagcgtt
    19681 aaacaaagat gttttaccaa cattaggatt tcctaaaata caataatttt ccattcgtcc
    19741 ggctcctatt cttctaatgc aatagaacaa gcatcgcaat gtctaatact taactgttgt
    19801 ccgtttactt caataataca tggcccttta aataaacatt tttgtttaat cgttatgata
    19861 gcgtcatctg ttaacccaaa ggcacttaga cgatacaaca tattctcatt agcaatatcc
    19921 attcgcttta ttttataagc cttattcatt tcaccatttt taatgtttaa catactattt
    19981 cgctctccta ttagaaataa taatcattat cacttaaaaa tcataaccct taaaattgta
    20041 gctcgcaata ctttatttaa ataattttca tttttcatgt aaaatttgtg acattgcaaa
    20101 aatgttttgt ataatcgctt ccatgcaaaa acccccactt caaatgtcat tgatagacat
    20161 ttaaagcgga ggcatgtaca tgttatttta tgatttattt cttaagtttt tttctaacaa
    20221 ttgattgagc ttttcaatat ttttattcaa ctgttctata ttcgtggctt gttgttttga
    20281 atttagttga tgcttgtttt gataatccat taaagcatca tataagaaca atgaacttgc
    20341 tgattgctgt ttcacttctt ggtacaatgc catcgtttta tcatctagta ctattgaacg
    20401 tgcatcatct tgtccatgta catcttgatg tttcgataca tttgttgcac cttgtggata
    20461 tactttaaag gttgtgtcgt atgtttcctt actatcttta gcttcagatt cctgtgattc
    20521 aaccgtttta tatttttcaa gtgcatgtcc ttcaatatca actcgtggaa taatacgatt
    20581 caaccatgct ggtaaatacc acgaaccttt tccaaacaat ttagttaatg caggaattaa
    20641 catcattctg actacgaagg catcaaagag tacaccaaac gctaatgcca tacccattga
    20701 tttaatcatg acatcttctt ggaatacaaa cgcaaagaag acactaaaca tgattaatgc
    20761 agctgctaca ataacaggac cactttcttt caatcctact ttgatagaat aatcattatc
    20821 ccctgtttta ctatattctt catgaattcg cgacataagg aagacttcgt aatccattgc
    20881 taagccgaat agtataccaa ttgtaataac cggcaagaag gctagcattg gtcctgtcgt
    20941 ttctataccg aataatcctt tcataaagcc atcttgcatc acaaaggttg taaaacctag
    21001 tgttgccatt aatgacaaga caaatcctaa aactgctttt aacggtatta agattgaacg
    21061 gaagacaatc attaataaga aaaatgccaa tacaacaatt actgttgcaa ataaaggtat
    21121 cgcctcattt aactttttag acatatcaat attaatgaca ctttgtcctg aaatttccgt
    21181 tttaaatcca tatttatctt gcgcatcttt attataatct cgtaaatcat gcactaagtc
    21241 atttgtactt tctgcattag gcccttgctt aggtatcacg accatcaaag cgtaatcatt
    21301 atctttactc atttgtggcg gcgtaacaat atccacattt ttcttatctt taatatcttt
    21361 atacacagat tgtaaatctt gttgtaatcc ttgtggatca tcttttttat ctttaacgtt
    21421 aatcagcatc ggtatttggc cattaaatcc ttcgccaaac ttatctgaaa taatatcgta
    21481 tgcctttttc tgagtagaat ctgctggctt tacgccatca tccggaatgc caagacgcat
    21541 atgactaact ggaatagccg ctgcaactaa tataattaaa cctaataata ctgctgcaag
    21601 tgcattacca gtaataaatt tagaccacgg cgtatcaata tcttttttaa aattagactg
    21661 caatttgttc actttaatgt gtttatggaa aatactaatt aacgcaggta ataaagtcag
    21721 tgcactgaat acagcaaata taacactaat tgccgaagca aaccccatca ctgctaagaa
    21781 atctatacca actagcgata aaccgcatac ggcaatcaca actgtcacac cagcgaagat
    21841 gactgcgcta cctgctgttc ccactgcaag accaatgcct ttaatgtaat ctgtttcagt
    21901 tttcataact tgtcgatatc tgaataaaat aaataatgca taatcgatac caactgctag
    21961 tccaatcatt acggctaatg tcagtgtgac atttggtata tcgaatgcat aagttaacaa
    22021 actgataata cctacaccag aagctaaacc aattaatgca cttataattg gtaatcctgc
    22081 agcaataact gaaccgaatg tgattaacag tacaacaaat gcaacaataa taccaactag
    22141 ttcagaatta ccgcctactt ctgtagatgt cattcctgta ccagttaact ctgtttgtac
    22201 attatgattg tcacgtaagt ctttcaaatg acttttaaca ttatctctag agccatcttt
    22261 taaagatgtt tgactaacgt catatgtgat atctgcaaat gcagttgttt tatctttatt
    22321 aatttgctta ttttcataag gatctgatat tttatcaatg tgcttgtcat cttttttaat
    22381 atcatctaac gttttcttaa tatctttagt aatactcggt tgcacaatac catcatcttt
    22441 agtcgtctta aagacaacac gtatttgtgc cttttcacta tcttgattaa aatgtttttc
    22501 aatcttttta ttcgtatcta acgactctag tcctgtcatt ttaatatcat tgtcaaattt
    22561 cggtgcattt gtagcaagtg gtatcaatat tgcagctaca atcactatcc atgcaatgac
    22621 cgcggaccat ttatattttg cgatgaatgt ccccatctta tataaaaatt ttgccaaagt
    22681 atattgcctc cttttaaaat caacgttgta gtttaactat acagtgtaga ttattgtttg
    22741 attatagtat ctatcaccga cctcttaaag aatcaattgg aaaattttgt atattaaact
    22801 acacacaaag gagaaatgta gatgaaagag actgatttac gagttataaa gacaaaaaaa
    22861 gcattgtcga gtagcttgct acaattgtta gaacagcaat tattccaaac gattactgtc
    22921 aatcaaattt gcgacaacgc actcgtacac cgcacaacat tttataaaca cttttatgat
    22981 aaatatgatc ttctagagta tttgttcaat caattgacta aagactactt tgctagagat
    23041 atcagtgacc gtcttaatca tccattccaa acgatgagtg atacgattaa taataaagag
    23101 gatttgagag aaatcgcaga attccaagaa gaagacgctg aatttaataa agtattaaaa
    23161 aatgtctgca ttaaaattat gcataacgat atcaaaaata atagagaccg tatcgatatt
    23221 gactgaaatg ctcccttcaa agtagacatt gaaaaaatga aaactttgaa gggagcttta
    23281 tttatgtcaa taaaacctaa agtagacata gatgtactgc tatatatgtt tcaagaatat
    23341 gaaaaaggcg tgtcgtttca atacttaatc gatacattac aattagatat taatgtcaat
    23401 actttgtatc ctaagtataa atattataaa acccatggta ttgaaacact acttacacaa
    23461 aaacaataca atcactattc aaaggaacta aaattaaaag ttgtaaatga atatttgaat
    23521 tcaaatcaat caacccaaga catcgcaatc aaatacaata ttcgtagttc gacacaagtc
    23581 aaaaattgga taaaaatgta tactgtagga aaagagatta agaatcaatc tcctaaaact
    23641 ggggtgtata tcatgaaagc aagaaaaacc acatttgaag aacgtgtaac aattgttgaa
    23701 tattacttga atcataaaca atcttataga gaagttgcag aacactttaa tatctcatac
    23761 ggtcaaattt accagtgggt tcataagtat caagcacatg gtaaaaatgg attagtagat
    23821 ggcagaggca aaggaaaacc caaatctatg atgacgccag aagaacaaaa agaagctgaa
    23881 attcaagcat taaaagcaca aaatcgatta ttagagatgg aaaatgacgt tttaaaaaag
    23941 tttcaagcat tggaaagaga gatgattcaa agagaaaaca aatcacggca tacaaaacga
    24001 tcgaagcgtt aaaacatcaa taccctatcc aacgcttgtg tcgtatttta ggcatttcta
    24061 gagcgagtta ttataagtgg gtacattatc aatcgagtga acttgaatta gagaatgaac
    24121 agcttaagcg agaaatagaa tcaatctatc ataagtataa tggtatttat ggctatcgtc
    24181 gcatttatat ctatattcgc ttaaaactag gtaaacaagt aaaccgaaag cgaatttatc
    24241 gtttgatgaa agaacttaat ttaaaagcag taatacgtca aaagcgaaaa ccttatcgac
    24301 gttcaacgcc acaaatcaca tcagaaaata agcttaatcg acaatttgac attgatacgc
    24361 caaataaagt ttggttaaca gatgtaacag aatttaaaat caaagaaggt agtaagatat
    24421 atttgagtgc catttatgac ttaggtgcta aacgtattgt tagttatgag ctaggccctt
    24481 ctaacaataa tcaattagta tttaaaacat tcaatcaagc gatagaaaaa gtagaaaata
    24541 caaaaggtat tttatttcat agtgaccgag gttttcaata tacatcgaaa acatttaaac
    24601 atatgttgga tgaatgtggc atgattcaaa gtatgtcacg tgttagcaaa tgtatcgata
    24661 acggtccaat ggaaggtgta tggggtacaa taaaaagtga aatttttaga ggaaataagc
    24721 actttaaatt taatagtgta gaagaagcta caaaaacaat tcatgatttt attcttttct
    24781 ttaatcatga aagaattaca ttaaaaatgg ctgattctgt ataacaacaa aaccaaccat
    24841 taattttaat tatttaattg tctactagac tgggtgcagt tcaaaaagtt gctactaaaa
    24901 aattgccaga ctttattttg agtctaggtg ctaaatttaa tcatcaagct aaagaaggta
    24961 aacttttatt agatatgaac cgcaatgtaa gtaacgaacg tgcaaaaaca ttacttggtt
    25021 gggaaccgat tgcgacacaa gaagaagcaa ttttagcagc aattgatagt atggctaaat
    25081 ttcatttaat ataatacaaa aacaccgtcc ataagtaagt ggctttcctc ttgataaaag
    25141 gggcattcac tcaatatgtg acggtgtttt atatttttac gttaacacta ttgctgtggc
    25201 ttttgacgac cttttaataa aattgctgtt gcacctacga ttataataaa tagaatcgca
    25261 ccaaataatc ccatatattt tactgcgtta ccgaacacga taccgacagc taaaaagtct
    25321 gtatctgaga atgttgttgc agcaccacct aattcgccta aaaatggcaa gaataataat
    25381 ggtaaaaacg ttattaggat accatttaga gcggcgccag caacagcacc tttaataccg
    25441 cctcttgcat taccgaatac agcagccgtt gcacctaaga agaagtgtgc aactacgcca
    25501 ggtaaaatga cgacgccacc aaataagaat aagataaaca taccgatgac acctgtaata
    25561 aagctgacaa agaatccgat tagtactgca ttttgtgcat aagggaacac aatagggcag
    25621 tctaatgcag gtttagaatt tggtacaagc ttttctgaaa tgcctttaaa tgctgggacg
    25681 atttcagcta agattaagcg aacgcccgtt aaaataataa atacaccagc agcaaatgtc
    25741 acaccttgaa ttaatgaaaa tacaataaag ttttgaccat cactaataga ttcgtgtaca
    25801 taactaacgc ccgcaaataa gcatgcgatg aagtaaagta atgccatcgt aatcgagata
    25861 ctaattgtac tttctcgtaa gaaacttaag ccttttggaa atttaatctc ttccgttgat
    25921 ttagacttac ctttgaataa ttgacctaca gcacctgcgg caaagtaact gattgagcca
    25981 aaatgaccta acgctacttg gtcattccct gtaatttttc gcatcgtagg ttggagtaat
    26041 gcaggtaaga ctgccatgat taatcctaat acgagtgcgc cgataacaat cgttagccag
    26101 cctttaatat gactgactgt taaaatgatt gctaaaaacg cagccatgta aaatgtatga
    26161 tgacctgtta aaaagatata ttttaaatta gtgaagcggg caattaaaat attaacaatc
    26221 atgccacaga ccatgatgag tgcagctgtt gttccaaaat cttttaaggc tagtgagacg
    26281 atagcttcgt tgttaggtac gataccttgc acaccgaatg cgtgttggaa tattttgccg
    26341 aatggctcaa gagatcgaac gacgacatca gcacctgcac ttaaaattaa gaagcccaat
    26401 atcgttttaa tggttcctga agtgattgtt gcggcaggtt ttttctgaac gattaaacct
    26461 ataaaggcaa tcagtgcaac aagaatggct ggttgactta aaatatcgac tataaaatta
    26521 aggattgctt gcataggtcg tacctccttt aaatcatgtt aagttgttgt aatttttctg
    26581 agagcttttg ttgtaattca gctttgtcta aaatattatc aagaactaag acatccccta
    26641 gacgttcggc attttcagct aaatctctac cacaaataaa caagtcagcc atctctggac
    26701 ttgctgtcat aatgtcgcta tgttcaactt cgatatcaga tggtgcatta agttgcctaa
    26761 gcgcttcttg tgcattcatt tctaccataa aactacttcc taaaccgtgg ccacatacta
    26821 ctaaaatttt catattaatc atgctccttt aaaatgtttt taatgtcttg tgcgtttgtt
    26881 gcagttaata gttgctggac tgtttggtta tcgcccagta cggttgctaa attttgtaat
    26941 acagataagt gtgaatgatt gtcgatggca ctcaatacaa aaatgagtga tgcgtagtga
    27001 tcttcatcac aaaatgccac atgttgattc aactttaata gacttaaacc aacttgatgt
    27061 acgtcattgt tcggtcttgc atgtgcaatt gcaatttcag gtgcgataac gatataaggt
    27121 ccaagttcat taacgctatc aatcattgct tgaacatagc cttgttcaat aatttgttct
    27181 tgtagtaatg gctgagaagc tatagttata gcttcagtcc aatcatttac ttgttctttt
    27241 acaatgatgc gtgttgttga caaaatgtct aatgacacgt tattaaacct cctttgtcat
    27301 agttaaagca atgtgttgtt taattttaaa aatattccca tctaagaaat cttgtcgaca
    27361 taagtcgttg cttaagcatt cgcttaactg tcccaatgcc tttaaatgtg cattggggtg
    27421 gtccgttgct aatgtaatta caaggtgaac gggatcgtta gctttactac caaaaataat
    27481 cccttcagtg aaataagtta gtgcgaaacc tacaccattc tgtacataat cagtaccagc
    27541 gtgaataagt gcaatgtgtg gactaatgac catatatgac ccgaattgtt ctaattgttt
    27601 taaaattgca gctgtataat ttgaattgac aatgccatca ttgattaaag gttgcacagc
    27661 cactgtaatt gccgattcaa ttgataatgg ttgtttattt ataatgatgc gatgttcagg
    27721 caataaatct gcgagtgact tgccatcagt tgccattttc atgactcgtt gttctcttga
    27781 gtcattgata atttgattca atttttgacg agattgctga ttgataaatg gatcgacatg
    27841 aataactggt acagctgaga tttcacaagg tactgttgaa atgacataat caatgttatc
    27901 ttgcaataat cgactttctt ccaattgata aatggaatag gcatcccaaa tgtgaaactc
    27961 aggatacaga tgatttagtt ttgattttaa aagttgcgac gtgcctatac cagaaccaca
    28021 tagtaagaca accttaatca ttgattgttt atgtgttgca acacgctcta tacttgatgc
    28081 gaagtgaatt gtaatgtatg ttaattcatc ttcgttgaag cgaatagcag catcttgttc
    28141 aattgggcta atatgcttgc taacggcttc aatgatttga ggatagcgac gcataacttc
    28201 ttgcctcaaa ggattaggtt gtagcatatc gtatttaata cgatgtatag ctggtttgat
    28261 atgtgtgata agactggtat gcaacttgtt gtcttttgac atatcaatgc ctaattcttg
    28321 gctaacacaa gtgatcaatt catgtatatt ttgcgataaa tcatggtatt caaaggtaat
    28381 tgaagatgct gtatgttcgg tcattttaga gcctagtaaa tgtaacgtga taaagataat
    28441 ttcagactct ggaaatgtga cattacaact gcgttctaag ttttctatca tttttgaagc
    28501 aatagcatac tgattagtat gtcgccattt atcaatttca ttgataggta tatcgaacga
    28561 aaaattttca tttaaacgct gaatggcaat gcgtatatga tagattaagc catcgatagc
    28621 cgactgaact aaatgataat tttcactatt taatgtctta ataatggcac ggcgaactaa
    28681 tgcgattgat tctgaattaa agatatcagc ctctataaaa ggtgcagctt gtttcatata
    28741 ttgatgtata aagtgtgcat acgctttacg ataatgatct tcctcaccaa taatattgaa
    28801 tcctttattg tggacataat ttaactttaa atggtattga tctagttggg cttgaatcat
    28861 tttaatatca tctgcaattg tccgacgcga aacattaaca tcttgcgcaa gttgctttgt
    28921 tgaaacagga tcagttgttt cgaataactt taaagcgata tgtgtgagtc gctcatcttt
    28981 tgaaaaatga atttgattcg ttagattgtg ctctaattca tttaatatcg ttgtgttagc
    29041 tgttgttact ttgatgcctg cagctttatt acggctgact tggtaatgat aagtttcagc
    29101 atattgctca atatatgcta tatcgtattg aatggtacga ggtgatacac caagttgatt
    29161 agcaatggta ttgattggaa taaacgtttg ctcatgaatt aaaagataca aaatttcaat
    29221 ttgtctataa cttaacaacg taatatcctc ctatttgtaa ttgtaagcga tttctcaaga
    29281 acgtagatat gcaatctctt tcatatttta atccgaaaaa ttgcatatca aaatgtttat
    29341 ggcgcaagat tttataggaa cttttaaaat aaattagata ttcatgttga caatttaaaa
    29401 atggcgcagt atatttagtt agacatctaa cgaaatggtg gtgcaataaa tggaattcac
    29461 ttattcgtat ttatttagaa tgattagtca tgagatgaaa caaaaggctg atcaaaagtt
    29521 agagcaattt gatattacaa atgagcaagg gcatacgtta ggttatcttt atgcacatca
    29581 acaagatggg ctgacacaaa atgatattgc taaagcatta caacgaacag gtccaactgt
    29641 cagtaattta ttaaggaacc ttgaacgtaa aaagctgatc tatcgctatg tcgatgcaca
    29701 ggatacgaga agaaagaata taggactgac tacctctggg attaaacttg tagaagcatt
    29761 cacttcgata tttgatgaaa tggagcaaac actcgtatcg cagttatctg aagaagaaaa
    29821 tgaacagatg aaagcaaact taactaaaat gttatctagt ttacaataaa tggtaagcgt
    29881 gactggtaga aatcagtcac tttatcttta atgttatagt tagatatcta attgttagta
    29941 agctaattat tggaaaagac aaggagtatt gaacaatgaa agacgaacaa ttatattatt
    30001 ttgagaaatc gccagtattt aaagcgatga tgcatttctc attgccaatg atgataggga
    30061 ctttattaag cgttatttat ggcatattaa atatttactt tataggattt ttagaagata
    30121 gccacatgat ttctgctatc tcactaacac tgccagtatt tgctatctta atggggttgg
    30181 gtaatttatt tggcgttggt gcaggaactt atatttcacg tttattaggt gcgaaagact
    30241 atagtaagag taaatttgta agtagtttct ctatttatgg tggtattgca ctaggactta
    30301 tcgtaatttt agttgcttta ccattcagtg atcaaatcgc agcaatttta ggggcgagag
    30361 gtgaaacgtt agctttaaca agtaattatt tgaaagtaat gtttttaagt gcaccttttg
    30421 taattttgtt cttcatatta gaacaatttg cacgtgcaat tggggcacca atgatttcta
    30481 tgattggtat gttagctagt gtaggcttaa atattatttt agatccaatt ttaatttttg
    30541 gttttgattt aaacgttgtt ggtgcagctt tgggtactgc aatcagtaat gttgctgctg
    30601 ctctgttctt tatcatttat tttatgaaaa atagtgacgt tgtgtcagtt aatattaaac
    30661 ttgcgaaacc taataaagaa atgctttctg aaatctttaa aatcggtatt cctgcatttt
    30721 taatgagtat cttaatggga ttcacaggat tagttttaaa tttattttta gcacattatg
    30781 gaaacttcgc gattgcaagt tatggtatct catttagact tgtgcaattt ccagaactta
    30841 ttatcatggg cttatgtgaa ggtgttgtac cattaattgc atataacttt atgtcaaata
    30901 aaggtcgtat gaaagacgtt atcaaagcag ttatcatgtc tatcggcgtt atctttgttg
    30961 tatgtatgat tgcggtattt acaattggac atcatatggt cggactattt actactgatc
    31021 aagccattgt tgagatggcg acatttattt tgaaagtaac aatgacatca ttgttattaa
    31081 atggtatagg tttcttgttt actggtatgc ttcaagcaac tggtcaaggt cgcggtgcta
    31141 caattatggc cattttacaa ggtgtggtta tcattccagt actatttatt atgaatgctt
    31201 tgtttggact aacaggtgtc atttggtcat tattaattgc tgagtcactt tgtgctttag
    31261 cagcaatgtt aatcgtctat ttattacgtg atcgtttgac agttgataca tctgaattaa
    31321 tagaaggtta aatatttcgt acacttctgg ctgagtatat ttcggtcgga agtgtatttt
    31381 tcaaataaaa aataaatata tgatacgatt atgaaaaaat aaagtgagat tggcatatgt
    31441 ataaatctaa aatactgttg aaatatattt ttagtgaaga atcagacgtt aaagatttaa
    31501 cggaagaaaa atataatcaa gattacgaag cattaacatt tagctttaaa gaggaaacat
    31561 atcaaagtag gttagcaaag aaaacaccga ctaaagcagg gtattttgtt acatgttgga
    31621 caaaagacga gaacaattgt aatcaaccat actcaaaaga ggcatttgca gattacttaa
    31681 tgattatagt tattgatgaa gaattaagcg gttatttctt atttcctagg gaattattgg
    31741 tagaaaaagg catcttaact acatttgagc ataaaggtaa aatggctttt agagtttatc
    31801 ctaagtggtg taatcaattg aataaaagag cagagcaaac acaaaagtgg caatgtaaat
    31861 atttttttga atactaatta aaaagtcata tggttgaact tataagagat gtatatatca
    31921 tctttcgata agataaccat atgactttat tttttatcat ttaatgatga acagtttctt
    31981 gtcctacttt attccaagtg aggataaagc tcaacattgc aaacacactg attgctgtta
    32041 ataaaataaa accgacatcc catccgaatt tatcaactac agcacctaag acgatgttgg
    32101 ccattacagc accaaacaga taaccaaata atccagttaa tccagctgct gtgccagctg
    32161 cttttttagg tacataatct aatgcttgta aaccaattaa cataactggt ccatatatta
    32221 agaaaccaat ggcaattaat gagacattgt ctaaccaagc attgcctgga ggatttaacc
    32281 aataaattaa tacaaatact gtgacaccta acataaagaa gaaaccagca ggtccacggc
    32341 gacctttgaa taatttatca gaaatgtaac cacataataa tgtaccagga attccagccc
    32401 attcgtataa gaagtatgcc caacctgatg cttttaagtc gaaatgtttt tcttcactta
    32461 agtagactgg cgcccaatca agtacaccat aacgcacgaa ataaacaaat atatttgcaa
    32521 aggcaattgc ccatacccat ttattgttca gtacatattt aaataaaatt tcttttgtag
    32581 ttaattctgt ttctaatgtt ttcttatcgc ttgtagcaaa gtcattttta taaatttcga
    32641 ttggaggtaa accttgagat tgaggtgtgt ctctaatcaa tatatatgaa attgcggcaa
    32701 tgataagtgc taagagtgca gggtaaatga atacaccttc aaaacctttt aaataaccaa
    32761 agttgataaa tgctgttgtt gtaatacccc aagcagcaat aggtgccata atacctccac
    32821 caacattatg cgcaacgttc caaagagcag tcttacttcc gcgttcactt acactaaacc
    32881 agtgaacgag aacacggcct gaaggtggcc agcccatacc ttgaaaccat ccatttaaga
    32941 ataataggac aaacataata ccgatacctg atgtaaagaa cggtacaaat cccattaaca
    33001 aattgacgat agcagtgagt actaatccaa gaactaagaa tatccgtgca ttgctccgat
    33061 cacttacagt acccataaag aacttactaa atccatatgc gatggaaaca gcagaaagcg
    33121 caaaacctag ttctgctttt gtaaaacctt gctcttgcaa tgccggcatc gctaatgaaa
    33181 agtttttacg taataaatag tacccagcgt aaccgatgaa aataccaaga aatacttgga
    33241 gacgtaatcg tttataggta tcatctatct gattttctgg caaaggctta atatgctttg
    33301 caggtttaag aaaattcata aaatcctcct taatatgtat ttatatgcat tttgtgcgat
    33361 aaacttatat tagacatgta tgaaagcgtt gtcaatatac ttttatgaaa tcttacatat
    33421 ttagttattg aagtatttta taaacattta gaaaaaataa attgctacac ataaatttat
    33481 aattatgcaa ttattatgtc gttatacata ctagctgtgt atctacgaag gagcgcaaac
    33541 atatttttat ttgcagaaaa gggctaaatg gcatataact atctttttta tataagttgg
    33601 tataaaattt atactaatag gagggatagt atgaatatag tagggcatca tcacatatcc
    33661 atgtatacaa aagatgcaaa acgtaataag gatttttaca caaatgtcct tggattacga
    33721 ttggttgaaa agtcggttaa tcaagacaat ccttcaatgt atcatttgtt ttatggggac
    33781 gaagtaggta cagccggaac aattttaagt ttttttgaaa ttcccaatgc gggtcataag
    33841 cagccaggta ctgaaacgat ttatcgattt tcattattag taccaaatca agcggcactt
    33901 cattattttg aaaaacgtct tgagaataat ggtattaagt ctgaacgttt gtactatctt
    33961 ggacaagaag gtgttgtttt taaagatgaa gacgacttag aaatcatact gcttgttaat
    34021 gatagttttg aagtaccaca tcaatggcaa cataacgctt atagtgaaat acctcaagca
    34081 tatcaaattt taggaatagg gccagtcgaa ttaagagtta gaaatgcagc gcgtacggta
    34141 gaatttttgg aaaatgtctt aggttatcgc aaaagagata ataaatcatt cgatgtgctg
    34201 acattagcac cacaaggttt atattcggat tttgtagtta ttgagcaaca gggacaacgt
    34261 gaaagacctg gacgaggtta tatccatcat attgcagtta atacaccaca aatgaatgac
    34321 ttagatgcaa tttacaagaa actacaacaa caaccacaaa gtaattcagg tataattgat
    34381 cgctatttct tggcagtgtg tcaatagaaa aatgagaatt tcttaatttt ttttaataaa
    34441 tattactaaa agagtacaga agaagaaaaa gaagcatttt actagtaata tctgtcaaaa
    34501 atcacagatt taaatagtat gcttcttttt agattatgca ttttatatac aaaatttatt
    34561 cgtcctgtcc ttccgcgctc attgtaaacc attcgcttag attaacctat attaattgac
    34621 gggttcgtgc ctcacacagt catttaatat aggttcacaa ttttgtgtgc ttacatggta
    34681 ctttaacatt tcctcactcg atttatagtc aaatatcatt cgaggccgtg tattaatttg
    34741 tttaacagct tcatctaatg acttttgtgt tatatttgca aaatccgtac ctttaggata
    34801 atactgtctt aaaagtccat ttacattttc aatggaacca cgttgatatg gacaacccaa
    34861 atcactaaaa taaatactac aattcaattc atcttctatt tgttcaaata gagcgaactc
    34921 cttacctcta tccatagtta tgctttttgg taataatggt ttcataatat ttataatctc
    34981 attacaaact acattggatt tacgtgtatt taatctacta gcatataaat atcttgtttt
    35041 tctttcaata atagttaaca agcaatatgt tgatggtcta acactccaaa cagtatcaat
    35101 ttcataatgt ccaaattctt cacggttatt aatacttttt gagccgatac tttataacac
    35161 gagcccctac gtctaatttg ccacgctttt cagaagactt attatatttt ttgcctgctt
    35221 gtctcatatc acgcttaaca tcaactttta ccaatccatt gcgaatatat ctatatatag
    35281 taggataact tatataatgc cctgtatctt tttcaatttg acttgctatc tgtctaggag
    35341 accattttaa tgaaagtttt tcttcaatat atgcttttgt ttcatcattc aaacgtctac
    35401 gtttagcaac ttgttcatct gaaatactac gcgattttat agcatcttcc tcagctaata
    35461 aaggattata aacaccatca acagaacgct tatttacttc tctataaaga accgcatcat
    35521 cacgacctaa aatcttagaa atctgaatga tgtttaaacc atcatttaaa tactttgcga
    35581 tagctagacg tgttttataa tctaattctt tacgtttttt tctttttttc atgtatatcc
    35641 tcctttactt ttaaaaagct tatttataaa ataagttata agaggatata tgttcatcaa
    35701 ctcccgatac caccgctaaa gcgtataggg agttgattct ttattttttc atattatttt
    35761 cttcataaac tggtttatga ttactttgat attctttttc attcatatct tcaactttta
    35821 catatttatc tgttgtctca ttctttttat ataaacttgt ccacactgta ccatctttat
    35881 cataaacaaa agaaatagtt ataaatttac catcttcata tactttaaaa tcactattac
    35941 ttttttcagg cttcttaaaa gaagactcac tcatactttt tctttcagaa tttaaaactg
    36001 catcaatctc tttatcatat ttctgaccac atgcagataa taatataact aatcctaaaa
    36061 ttaatacaaa ccatcgcctc atgataaatt cactcctttt tacgtaaatt atatcattgc
    36121 tatcgtcttg aaacaaacta acgtcctaga ggttttaaat tgaaacgttt taaattatca
    36181 atacgccaac cttcagctgt acgaactaaa tccaattgtg cagatgtttg acgatgttca
    36241 attttatcat ctccatctgt ttcaatacgt tcgtcgaact gaacaaaaac tttataacta
    36301 tcttttttag gatcataagt atcaaaataa acatgaatat cattaaccgt tatatcatca
    36361 tataaatcat atttatcttt agaatcctta aataatgtat cgtaataatc ctttgtaact
    36421 aaaggcttta tttgtggtgc ttttgattta aaagaagaat cttttttcat ttcaaacatc
    36481 tgtttaacaa aatttgaaga aacttcacgt atttgctttt cttcatcaga aataactgtt
    36541 tttgattgtc tctttactaa tttatttaac tctccatttt tatctcttaa tatacgttgt
    36601 tcttcctgta aatcttcaat ttgattttgt ttcattttaa cttgttgttt aggaatctga
    36661 taaccaatta aaacagcaca aaataatatt actgttacaa gagtaaacat aataattttt
    36721 tgcttcattt attttcactc cttatcatga ataaaactat atccatcagt cattttcaat
    36781 gtacgttcat taactttaaa agcaccttcc caattcattt ctgttattct aaatgtttta
    36841 ccaccatcac ttacaccaat aacaacagcc acatgaccat aatgcccatc atgaccgcct
    36901 gcaccttgtt cagcaataaa taatgcacca ggttccggat ttttatcaac tttataaccc
    36961 tcagccttag ccctatcatt ccatttatgt gcatcatgcc aataagttga tacaggttta
    37021 cccatttttt gacgcatacc gtatgcatac caagtacatt gaccagcagt gtataaatta
    37081 ccttttccag ggtcatcatt tgaccaatgt ggcaatttac cattaaactt tatagacttt
    37141 cctttaccat tactacatga cgttttagct tctgaaccgc ctaaatcttt cataattttt
    37201 tcaactgttg gtatccaacg ggcattcata ttattagggt catttgacgc accaacaggc
    37261 gcatatttgg gacctatttt tttaggagta tctaaacctt tagcaatata aacatcatac
    37321 aaatttttag ctcccgcatt aagaccgtct tcaatagttg gatatgttga atcatgaatc
    37381 gattttgaac ccataacaga caaaggatta ttttgtcttg tcgcattttc tcctttaccc
    37441 cattgtgatt cactcgcaat aatagccata aaaatattca caggtacttt atttttttct
    37501 gcaattttct gtatttcttt tcctttaccc tctaattttc cacctttagc attttcatta
    37561 aacttatcaa taccatttgc cgaaacatta ccgccactaa cagtacaacc agcatcatct
    37621 ttatctttat ttgaatcagg cgctaacaaa aacattacaa ttactggaaa tgtaaaaaaa
    37681 gccaccacac acaaaatgat gatgactttc gctaatttca atttaacacc tattggcgtt
    37741 ttacctatta cttttttagc aatatctttt ttatttacca tgcttattga tatccttttg
    37801 ctcttttaga cgatcttttg actgagaatt acgtgtaata ggttgattac gatttccaga
    37861 ttgtctactt tgttgattct gtgtcgcatt ttgtatttgt cgtttttgaa catcagtatc
    37921 ataagcagtt tgaactgact tctgttcttt acgatttatt gaatgattct cattatttaa
    37981 tggctgatta accacttgtg gcgacctttg tatatctttt cgctgtcttg tttctcgtac
    38041 ttgatgttct tgctgtgatg ctttttgtgg ttgtttagaa taattatttg cattcgattc
    38101 aacagaagca gattttacat cgttaccatt cattgactct tgttgttctc ttcgcttatt
    38161 accatttttt tgattttctt tatttataaa taaaggcatt ggtttatact gctgtcttct
    38221 tcttaaatca ttatcatttt gaagtggtgg ggcgggttca tctctttgat tttctcgttt
    38281 agctttctta ttttgcgctt gttcatgacg aatcttatta aaacgttctt gtgttcgatt
    38341 catcacacca gcaccaattg cactaggaga aaaactaata ttaccacgac ttacaaaatt
    38401 gataatttga gcccgttttt tatacattac aaacaatatt acaattataa atattgaatt
    38461 taaaaaataa gcattttgac tatccggatc aataaaacta tttgcaagct tgatacacaa
    38521 accaactatc acaaataaga atgtcataca ggcacttaaa ataatatagc ctaacgtttt
    38581 tgcaaacgca gagaaaacag ataattcaaa tcctggtata aaactcacta tccaactgaa
    38641 tactaaaaat aagaataaaa ataaagcaaa tgtagaaaaa atcaacttcg caaaacttat
    38701 tgataaaaat actatcgaca taaaaagtgt aataataaag ccaatcactg aaatagccaa
    38761 cttttgaaat acataacctt gcgtcatata tttattattt ttcttttcaa cctcatcttt
    38821 aacaatatca tctaattctt tttctgattt tttatcataa tcaagtttta aaatgttatc
    38881 aatacggtct ttaccttttg cttttatctc tttttcgtct acagttccat aattcattac
    38941 taaatatgtt ctatatatcg tcatatcaaa taattgatta cgtatatgat tcatacctga
    39001 attctcatga atataatcaa tattaccatc aatagaagat gtactattca taattatatt
    39061 atttaagtcc ttaccaatat tgtttgtacc acgtataata ttacctgcat tcgcaagtat
    39121 accaacacca aaaccaatta aacacataat taaaaacgcc cgttttaatg cttctcttgc
    39181 attaccttgt acactaaaaa taataaatgc gttaaaacaa acaataataa ataatgttat
    39241 tgccaaatta ctcaataact tttgatatat atcgccaaca aaatcgccga ttttatccgc
    39301 aaaatcatta ataacatcta gttgaaataa attttcaatc gcataatctg taacacctgc
    39361 taaaatctta gtcatagaaa agaaaaaatc tgacacatta ttaatttgtc gtgaaatttc
    39421 ttcttgacca aacggattcc agtcgccttt ttcactatat attgaacgat aatgtgataa
    39481 atcatacttt tcaattttgg gcttaactgc atgtgagggc tctccaactg gattagaaac
    39541 agcgtaagca ctcacagatg ttaaaagcag aataggcaaa attaaaaagc ataaaagata
    39601 cacactttta tgcttcatca aatcactcct tatttaatat ttgtttcaca tcttccacaa
    39661 aatcatattg ttccattaaa ggtgcatcaa ataaaattgg ttcattatat tgtccaagta
    39721 tagttatata acctcggccg atatcactat catgaatcgt ttgaaattct ttatcagttt
    39781 gaccaaaggt catacgataa ccatcatttg acaaattacc taaagacaca cgtaacccta
    39841 attggtcacg cacattacct ttaatcacat ctgcatctgg tctttgtgct gttaaaaaca
    39901 taaatacacc agcttgacga cctttcataa taatattaat taaataatca ttcatttctt
    39961 gtaattcttt tttatctaaa gttgaagtaa aagccgttac ctcatcaaaa ataatgaaat
    40021 aggggcgcat atcaaaatta cggaaatcat ttccgatttt ataatccgaa ctatcattca
    40081 ttaatctaaa acgctcttcc atttcattat tagcttctct caattgtttt aatatttgac
    40141 ctttagtatc tgcaacttta tcatcaccaa taacgcgctt cataaagctt aaatctgaaa
    40201 cttttgggtc taataaacgt acttctgaat gtctacggaa taactcacga atcacataaa
    40261 ataaaaaata tgttttacca cctccggtta tacctgtaac caatccatga ggtgctttca
    40321 caaaatccca agtaatttga tgcgtaatag caatttttgt atcataaaaa attgtatttg
    40381 attcttgttc gccattcata acaatacgct ttttatattt aagtggcata aattcatatc
    40441 tcaaataacg accaatatgc tttttttcta ctaattcaga attaaacatt ggttcaataa
    40501 tcgtttctaa ctctaaataa ttatcttgaa acctcgaacc gtctttttta atatgaataa
    40561 caaaagtttt tttaccacgt ttataaaaca tcgttggata atagtcatat tcagtttgtt
    40621 tataatcaat agggatatac tcggttgttt tcttgattac tgtacgatta tgaaagcctt
    40681 gtgtttcaat catttcagct aaatcttttg ttttaaaacg aattaaaagc tgagcgataa
    40741 gcttacatat aacaaatata attgctgtac caattaataa atacaataaa tgcggtttaa
    40801 aatatgcaaa caacgtcaat atatccaatt ttttattaac tgatttataa tcaataaaca
    40861 aaatgtatat acgatatacc aaaaaaataa atgctaatac acttgcaaac ataaaatcaa
    40921 tatacttctg ataaggctgt atacgtacgc ctttaaataa agcaatttca ttcattgcca
    40981 tcaaatccac tcctttaaaa taaaaagcac atacaatttt gtatgtgcta aaaattacat
    41041 cgcaaatttt tcttcagctt tagagcttgc agaatcttct tgtgttctaa atgcttcagt
    41101 catttcctca aatggacaat gtacaaccat ttttccaaca cgtccataaa aatcactgaa
    41161 tatacattga cctttcttca aatctttcaa catctccata ttttcaggag attcttttga
    41221 atattctaag cctaaagatt ttaaaatatc ctcacgttca tctttttcat caaatgcaaa
    41281 atgacaacca aagttaccac catcatcatc tgctttatct tttaccgatt gtgtaataaa
    41341 tacaagtgaa ttattctcag aacgtcccgt tctttttata cgcttaccaa cttttttacc
    41401 ttgtcttgaa gcactaaaag cccaaccctc atcaataaat atagtcgttt gaacatctct
    41461 acgagaaccg aatttctcta aaaacttacc aattaataac attaatgtaa tcccattttt
    41521 ctcacttgtt gaataacttg atgtatcatc atctgcttta ggcatatcta aacctttaac
    41581 ctgtaaaata gttgcttttt cttgaatatt taaagctgga ttcgtaccat cactaaaaac
    41641 aagttttaaa atattgtttt gcgttttttc aaataataaa tcgcctgcat tttttacatg
    41701 ctcatcttca ttattctgta atttttcaat tacatttaat gaaccaactt ttttaccatt
    41761 ttcacgttct tcaaccgtat cagaaatagc ttgataaatt gctgtttcaa tatgatgata
    41821 attttcaaaa gatttaattt catcaaacac tgatttcaac atctcattag cttcaatacc
    41881 atctaaaaat acaatagggt ctagacaacc ataattggtt gaatcttcat aattcaatgt
    41941 tacatattta aatgttttta aatgattaat aaatagggga tacttttctt gcatttcctt
    42001 actttctgtt actctgtcaa accattctgc aaactcagat tttggatccc agtacaacat
    42061 ttgaccttta aaaaatgatg tataaatcca taataatttt gctaaaaatg atttaccttt
    42121 accagtttga ccagtaatta aaacatgcgg actatcactg acagccccct taataccttt
    42181 tgcggccaac aataaactaa acaaaattat atcccttgaa cttgcaacag cttcctcacg
    42241 tgaaacagaa cgtataaatt tatcaatacg ccctatataa aaacccgttt ttgttcccaa
    42301 actttgagac acaccaaata aattctctgc tatacctgtt gccgttgttc tttgtaacca
    42361 atacttatta ataaataaat cattaccatg taaacattga taaaacaatt gtaactggtc
    42421 agctaatgga ttaactgcat atatttgagt ttcctttaat cgtttcatca aacgctttgc
    42481 tttacgttta caatctctat aatcatcact tttaattaca aacataaata cccactcaat
    42541 aaatacacct gagccagtca cttctcgttc catttgattc aatacaaaca aattatcttg
    42601 aacatcttcc gaagcactgt cgccagtatc ttgcttttct tttgcttgtt gtttatgatt
    42661 tattttcgca cggtcaaatt ttttcttaac tttctcatta ttaatgattt ttgcttttac
    42721 atggcattca accggaaaag gcatattttg agctttctca aataaatgtg tatattccat
    42781 atctaacgga aaatcatcaa caacaactgt tgttgtataa aatttctcgt tatcattttc
    42841 aacttctaaa acactcaagc gagtagggtc aattactgca ttataaatat ctgtaatagc
    42901 tcgtcttgaa gattcttcct gtacgtcatg tgttgcattt gatataaatg gcttccgaat
    42961 aatataagcc agctcattct cagttaaacg ttcgcctcca atacctagaa aactatcagc
    43021 caaatctcgt tcaaatgttt tatacttatc taaaatttta tcgtctaaat tcacatcaaa
    43081 accaaaattc gaaacaatac gctcagccgt atctgataat gtacttttta aaatctcttt
    43141 aagtgtaact gcatcgtcag ctaattcacg tattttaaca cccataacaa aagaattatg
    43201 cgtaatcatt tgtaattctt gttctaatac gtcaatagtt tcttttgaat aatattcagc
    43261 tacattaaaa gcttcatcat caaaatcttt actaatttct ttaaatttat ttcgcaaatc
    43321 taaatcatgg tcatacattt caaaatgaaa atcttgataa cctttaatcg actctaaaaa
    43381 ataacgcata cgcttttttg attcttcttt tgcatcataa tttgctgttg atatagactc
    43441 agaacgaaca cgataatacg cccaaacttc gccagttttc gttaataaca aattatctct
    43501 taaacctgca tacggtgctt ttaaattcat gtcatccctc caataaaaat aaccccaata
    43561 ggggctattt aaatgttatt tttttatcat gatattctac ttcttcatca taacttaatt
    43621 taacttttct taactgaaca ttaaaaacaa atattacaaa atcccataag aagaaaaata
    43681 atttctttcc atcttgtttt aacttaacaa tatatttcgt tatataataa ggtgcaaccg
    43741 taaagtaaat aaacataagt ccaccaatcg acataattac aggctgtaat aaataccaaa
    43801 ataacaaaaa taatataaac cacacaatca aaaatgtagc ccaaaaatct aaacgaaaac
    43861 ccttattaat acgaaactta tctgtaaatt catacataac tatcggttgt tcaaaagttc
    43921 gctttaaatt ataagctttt ttatccaaat ggaatcacct caaccgccaa aaatcatttt
    43981 ccaaacacct gcaatcgcat taaatacctg ttcaggacct ttagaaacta agaataaaaa
    44041 gccaccaaca acaaataata taaccatatg cccatattta ccactagccc attttacaat
    44101 accaacaacc aatactacta aaccaatacc cgtacctact tcattactta tccagtcacc
    44161 aactccacca agtgatggac ggtcagcacc taaaacaaaa aaattaaata aaccactcat
    44221 aacattaacc tccaatagaa ttagtcatat ttttaacgta atatttacca tctttttttg
    44281 ttatatctaa tgtgtaatgc tccaaattct ccaaaggaga atctttatct ttcatcaaaa
    44341 tttcaacttt agcaacataa tcatcgcctt ttggatataa cctaatttct cttatctgag
    44401 aaacttctct agttccttct aagccctcag ggttatccat taaataggcc atatcatcag
    44461 acttactaga agcatattta ttaaagaaat cttcaataaa agcttttgct ttcggattgt
    44521 cttcccgttt attgtctttc aaattatcct caaccatttt agctttattc aattgactat
    44581 cagggattgg cgtaaaataa ggatattcaa caacaacata cttattattt tcactcttaa
    44641 taggtatatt tattaaaatg ttttgattta ctttacgttg cttttcttct gtttttgttt
    44701 catactcatc tttatctttt tcacttttct ttttcttctt aactttaact tctttctttt
    44761 cagtaatatt cacatcataa ttaacaatat actgtataat tgtttgttta tctttacgtt
    44821 taatattata aaattcttta ctattcaatt ttcgttctgt atctgaaatt ttttcatcag
    44881 gttttttata atctgatgga aaatatttca acaattcttt ttctctagat tccaattctt
    44941 ttgaatcttt cgagatattc atatacgtat caataaactt atcagcatat aactttaatt
    45001 taggactata ctgaactgta ttcgcattat cttcatactt tttttgaatc atattcgttt
    45061 tattaactgc ttctttactg tcattatttg ctcttgtagc tttaataaaa gaagctaata
    45121 aaagaattaa caaaatagct aataatgagt aaaaacaaat tacaatcatc ttacgacgat
    45181 ttttaaattt tggaattact cggcgttgtt ctttaggaat atcaaaatgc tttccgtttt
    45241 tagaaaatcg tgttaaaatg tacttgctta ttattttcat aaacgatttt ctaaaatcct
    45301 ttacgaaatt tttaataaaa atcacatccc agttattttt ttaacactta tcttttgata
    45361 agtgcttttt ttgttttaaa gcctccaaat aatgattaat tctattcgta tgctcatcat
    45421 taaattgtga atgcgctaaa attttatcta aaaaatcagt atctgtaagc aacccctcag
    45481 attgcgccat actttcggca gttaacactg tatctaaagt agtagcaact tgtttacata
    45541 accaatcaag attatcctca atggacttta atgttggtct agtagttaaa ttcaattttt
    45601 cagtatcttt aataaataac gcccaaggtc tatataatgg ccaccgctta cgattttt
//
LOCUS       DAKSQE010000006        42801 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.006, whole genome shotgun sequence.
ACCESSION   DAKSQE010000006 DAKSQE010000000
VERSION     DAKSQE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 42801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42801
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.006"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..1377)
                     /locus_tag="O0V33_000228"
     CDS             complement(<1..1377)
                     /locus_tag="O0V33_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCX8974919.1"
                     /translation="MKNTLYHQLYEKLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTT
                     VEHAYQLLLDEGYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYKPKASDEAYDY
                     AFNLDEIDTKHFPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGV
                     ICDPNQIIIGSSTEQLVNQLVDLLYTSTFIIEKPSYPPIKNILDKKQVEYEQIEVEDN
                     GINVDEVIKSQKNIVYITPSHQFPTGYVMDLKKRTQLIQWAQEKEERFIIEDDYDSEF
                     RYFGKPIPAIQGLYSRGEKVIYISTFSKSIFPSCRVAYMVLPYSIMKKYHSQNHIEGN
                     TVPVHMQNLIATFISSGGFERHLNKMRRIYRRKLTYILKRLKPYKEQLDIQGAETGMH
                     FTITVKNGLTLQECLDRANKVKLKLQVYNFDDDQDYKKTPKFILGFGGIDDDALKPHV
                     DALIKSLII"
     gene            1481..2368
                     /gene="pdxS"
                     /locus_tag="O0V33_000229"
     CDS             1481..2368
                     /gene="pdxS"
                     /locus_tag="O0V33_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HCX8974920.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQDRGW"
     gene            2372..2932
                     /gene="pdxT"
                     /locus_tag="O0V33_000230"
     CDS             2372..2932
                     /gene="pdxT"
                     /locus_tag="O0V33_000230"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HCX8974921.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(3139..4353)
                     /locus_tag="O0V33_000231"
     CDS             complement(3139..4353)
                     /locus_tag="O0V33_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCX8974922.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            4511..4972
                     /locus_tag="O0V33_000232"
     CDS             4511..4972
                     /locus_tag="O0V33_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCX8974923.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            4991..5557
                     /locus_tag="O0V33_000233"
     CDS             4991..5557
                     /locus_tag="O0V33_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HCX8974924.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLVE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            5547..6554
                     /locus_tag="O0V33_000234"
     CDS             5547..6554
                     /locus_tag="O0V33_000234"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HCX8974925.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            6568..9024
                     /locus_tag="O0V33_000235"
     CDS             6568..9024
                     /locus_tag="O0V33_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCX8974926.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            9508..10872
                     /gene="radA"
                     /locus_tag="O0V33_000236"
     CDS             9508..10872
                     /gene="radA"
                     /locus_tag="O0V33_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCX8974927.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELVGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSF
                     ALHS"
     gene            10897..11970
                     /locus_tag="O0V33_000237"
     CDS             10897..11970
                     /locus_tag="O0V33_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCX8974928.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIIGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
     gene            12527..13981
                     /locus_tag="O0V33_000238"
     CDS             12527..13981
                     /locus_tag="O0V33_000238"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCX8974929.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMEISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
     gene            14405..15052
                     /gene="cysE"
                     /locus_tag="O0V33_000239"
     CDS             14405..15052
                     /gene="cysE"
                     /locus_tag="O0V33_000239"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCX8974930.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            15036..16436
                     /locus_tag="O0V33_000240"
     CDS             15036..16436
                     /locus_tag="O0V33_000240"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCX8974931.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQGLNQTVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGA
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKKADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            16429..16833
                     /locus_tag="O0V33_000241"
     CDS             16429..16833
                     /locus_tag="O0V33_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCX8974932.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNEIITIVNER"
     gene            16841..17587
                     /gene="rlmB"
                     /locus_tag="O0V33_000242"
     CDS             16841..17587
                     /gene="rlmB"
                     /locus_tag="O0V33_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCX8974933.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLSTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            17587..18111
                     /locus_tag="O0V33_000243"
     CDS             17587..18111
                     /locus_tag="O0V33_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCX8974934.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARMQLIDTIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIT
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            18192..18761
                     /locus_tag="O0V33_000244"
     CDS             18192..18761
                     /locus_tag="O0V33_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCX8974935.1"
                     /translation="MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSAEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRYDVENNYLNKLAIKELIRQFKYLSAFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            18876..19019
                     /gene="rpmG"
                     /locus_tag="O0V33_000245"
     CDS             18876..19019
                     /gene="rpmG"
                     /locus_tag="O0V33_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCX8974936.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGTATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            19075..19257
                     /gene="secE"
                     /locus_tag="O0V33_000246"
     CDS             19075..19257
                     /gene="secE"
                     /locus_tag="O0V33_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCX8974937.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFG"
     gene            19270..19818
                     /gene="nusG"
                     /locus_tag="O0V33_000247"
     CDS             19270..19818
                     /gene="nusG"
                     /locus_tag="O0V33_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCX8974938.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            19999..20421
                     /gene="rplK"
                     /locus_tag="O0V33_000248"
     CDS             19999..20421
                     /gene="rplK"
                     /locus_tag="O0V33_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCX8974939.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            20629..21318
                     /gene="rplA"
                     /locus_tag="O0V33_000249"
     CDS             20629..21318
                     /gene="rplA"
                     /locus_tag="O0V33_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002020168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCX8974940.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASL"
     misc_feature    21409..21555
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            21593..22093
                     /locus_tag="O0V33_000250"
     CDS             21593..22093
                     /locus_tag="O0V33_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCX8974941.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEENAE"
     gene            22136..22504
                     /gene="rplL"
                     /locus_tag="O0V33_000251"
     CDS             22136..22504
                     /gene="rplL"
                     /locus_tag="O0V33_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCX8974942.1"
                     /translation="MANHEQIIETIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
     gene            22679..23287
                     /locus_tag="O0V33_000252"
     CDS             22679..23287
                     /locus_tag="O0V33_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCX8974943.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKLNGIDNVIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
     gene            23502..27053
                     /gene="rpoB"
                     /locus_tag="O0V33_000253"
     CDS             23502..27053
                     /gene="rpoB"
                     /locus_tag="O0V33_000253"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCX8974944.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEFNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            27217..30813
                     /gene="rpoC"
                     /locus_tag="O0V33_000254"
     CDS             27217..30813
                     /gene="rpoC"
                     /locus_tag="O0V33_000254"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCX8974945.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIRHPETDEVI
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            30950..31204
                     /locus_tag="O0V33_000255"
     CDS             30950..31204
                     /locus_tag="O0V33_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HCX8974946.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            31302..31715
                     /gene="rpsL"
                     /locus_tag="O0V33_000256"
     CDS             31302..31715
                     /gene="rpsL"
                     /locus_tag="O0V33_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCX8974947.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            31781..32251
                     /gene="rpsG"
                     /locus_tag="O0V33_000257"
     CDS             31781..32251
                     /gene="rpsG"
                     /locus_tag="O0V33_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCX8974948.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            32374..34455
                     /gene="fusA"
                     /locus_tag="O0V33_000258"
     CDS             32374..34455
                     /gene="fusA"
                     /locus_tag="O0V33_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCX8974949.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            34672..35856
                     /gene="tuf"
                     /locus_tag="O0V33_000259"
     CDS             34672..35856
                     /gene="tuf"
                     /locus_tag="O0V33_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCX8974950.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(36137..37312)
                     /locus_tag="O0V33_000260"
                     /pseudo
     CDS             complement(36137..37312)
                     /locus_tag="O0V33_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
     gene            37483..38670
                     /locus_tag="O0V33_000261"
     CDS             37483..38670
                     /locus_tag="O0V33_000261"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCX8974951.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMASTELHDKLWDNAQYLKNGLSKLGYDTGESETPITPVIIGEEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     NLI"
     gene            38946..39824
                     /gene="hchA"
                     /locus_tag="O0V33_000262"
     CDS             38946..39824
                     /gene="hchA"
                     /locus_tag="O0V33_000262"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCX8974952.1"
                     /translation="MSQDVNKLSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPAKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALENDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQSLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            39982..41619
                     /locus_tag="O0V33_000263"
     CDS             39982..41619
                     /locus_tag="O0V33_000263"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCX8974953.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIEGELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPVNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFD
                     KVDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKDTMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAANRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYTNVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG"
     gene            41833..42798
                     /locus_tag="O0V33_000264"
     CDS             41833..42798
                     /locus_tag="O0V33_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCX8974954.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFTRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IDAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPDFKLEYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLVSMTKLMLEAIEQKDTVKNNN"
BASE COUNT        15335 a         6276 c         8341 g        12849 t
ORIGIN      
        1 tataattaat gattttatta gagcgtctac atgcggtttt aaagcatcat cgtctatacc
       61 gccaaagcct aatataaatt taggggtttt cttatagtct tggtcatcat caaaattata
      121 aacttgtaat tttaacttta ctttatttgc tctatcaaga cactcttgta atgttaatcc
      181 attttttact gtaattgtaa aatgcatacc tgtttcagca ccttgaatat caagttgctc
      241 tttgtatggt tttaatcttt ttaaaatata ggttagtttt ctacgataaa ttcgtctcat
      301 tttatttaaa tgcctttcaa aaccaccgga agatataaac gttgcaataa ggttttgcat
      361 atgaacaggt acagtgttgc cttcaatgtg attttgagaa tgatattttt tcattataga
      421 atagggtaac accatatatg caacacgaca gctaggaaaa atagactttg aaaatgtact
      481 gatataaatc actttttctc ctcttgaata tagaccttga attgctggaa taggtttacc
      541 gaaatatcta aactcggaat cataatcatc ttctataata aatcgttctt ctttttcttg
      601 agcccattgt attaattgag ttcgtttttt taaatccatt acatatccag ttggaaattg
      661 atgggaaggc gttatatata ctatattttt ttgtgattta ataacttcat ctacgtttat
      721 tccattatct tcaacttcaa tttgttcata ttcaacttgt tttttatcta aaatattttt
      781 gattggtgga taactaggtt tttcgataat aaacgttgaa gtataaagta aatcgactaa
      841 ttgatttact aattgttcgg tagatgaacc aattataatt tgattaggat cacaaattac
      901 gccacgatta gtaaataaat aaaatgccag ttgaaaccgc aaatgtaatt ctccttgaaa
      961 gtgtcctcta cgtaattgat ttaaatgatt tgtatcataa agatctttgg aatactttct
     1021 gaaaagttct atagggaaat gtttcgtatc tatttcatcc aaattaaaag cataatcata
     1081 agcttcatca ctcgcttttg gtttatatga atcatcatca aaaagagaag ggataggttg
     1141 attgtttaaa attgttaaag attcaatttc ggacacaaaa tatccagagc gaggtcttga
     1201 ataaatgtaa ccttcatcta atagaagttg atatgcatgt tctacggttg tttggctgat
     1261 agataaatgt ttgcttaatt gtcttttaga ataaaattta tcaccttctt taaattgacc
     1321 ttcaattatt tgttttttta atttttcata aagttgatgg tataaagtgt ttttcaattt
     1381 tataactgac ctcctaaatt tgtcttattt tgtacctttt taaatatcag tttatacatt
     1441 acaatgtatt taatcaactt gaaaaggggt tttatgtata atgagtaaaa ttattggatc
     1501 agacagagtc aaaagaggta tggctgaaat gcaaaaaggc ggcgttatta tggatgtcgt
     1561 taatgctgag caagcaagaa ttgcagaaga agctggcgcg gtagcagtta tggcattaga
     1621 acgagtacct tctgatatta gagctgctgg tggcgttgca cgtatggcaa accctaaaat
     1681 tgtagaagaa gtaatgaatg ctgtttctat tccagtcatg gctaaagcac gtattggtca
     1741 tatcactgaa gcaagagtat tagaggcgat gggtgttgac tatattgatg aatcagaagt
     1801 gttaacacca gcagatgagg aatatcactt aagaaaagat caatttacag taccatttgt
     1861 atgtggatgt cgtaatttag gtgaagctgc gcgtagaatt ggtgaaggtg ctgctatgtt
     1921 acgtactaaa ggtgaaccag gtacaggtaa tattgttgaa gctgtaagac atatgagaca
     1981 agttaattca gaagttagtc gattgactgt aatgaatgat gatgagatta tgactttcgc
     2041 gaaagatatc ggtgcgcctt atgaaatttt aaaacaaatt aaagacaatg gtcgtttacc
     2101 ggtagttaac tttgcagctg gtggcgttgc gactcctcaa gatgctgctt taatgatgga
     2161 attaggtgcc gatggtgtat tcgttggatc aggtattttt aaatcagaag atccagaaaa
     2221 atttgctaaa gcaattgttc aagcaacaac acattaccaa gactatgaac taattggaag
     2281 attagcaagt gaacttggca ctgctatgaa aggtttagat atcaatcaat tatcattaga
     2341 agaacgtatg caagatcgtg gttggtaaga tatgaaaata ggtgtattag cattacaagg
     2401 tgcagtacgt gaacatatta gacatattga attaagtggt catgaaggta ttgcagttaa
     2461 aaaagttgaa caattagaag aaatcgaggg cttaatatta cctggtggcg agtctacaac
     2521 gttacgtcga ttaatgaatt tatatggatt taaagaggct ttacaaaatt caactttacc
     2581 tatgtttggt acatgcgcag gattaatagt tctagcgcaa gatatagttg gtgaagaagg
     2641 ataccttaac aagttgaata ttactgtaca acgaaactca ttcggtagac aagttgacag
     2701 ctttgaaaca gaattagata ttaaaggtat cgctacagat attgaaggtg tctttataag
     2761 agcgccacat attgaaaaag taggtcaagg cgtagatatc ctatgtaagg ttaatgagaa
     2821 aattgtagct gtccagcaag gtaaatattt aggcgtatca ttccatcctg aattaacaga
     2881 tgactataga gtaactgatt actttattaa tcatattgta aaaaaagcat agcttaatgt
     2941 atgctaaatc aacgaattat tgatatttat agatttgttg agaagaaaat tactccttca
     3001 aacttagctt tggaggagtt attttttatg tcaaaattaa aaatgataaa agataaagat
     3061 atacataaga aaaaaccctt caaagagact gagaatagtc aaaattttga aggggttaat
     3121 tcgatgttga tgtatttgtt aaataaagaa tccagcgatt gcagctgaaa tgaaagatac
     3181 tagtgttgca ccgaataata atttcaaacc aaagcgggca actgtatctc cttttttgtc
     3241 attaagtgat ttaatcgcac ctgaaataat accaatagag ctaaagttag caaatgatac
     3301 taagaataca gatgtaacac cttttgcgtg ttcagataaa tcactaagtt taccaagtgc
     3361 ttgcattgct acaaattcgt tagataatag ttttgttgcc ataactgaac cggcttgaac
     3421 tgcatcttgc catggcacac ctactaagaa tgcaaatggt gcaaagacaa aaccaattaa
     3481 tgtttggaaa tcccaagaaa tagcgccacc tgaaactgta ctaaagatat tgcttacaat
     3541 tccatttaat agagcgataa tggcaatgta accgattagc attgcgccta caatgacagc
     3601 tactttaaat ccatctaaaa tatattctcc tagcatttca aagaatgatt gttgtctttc
     3661 ttcagtttct tcaactaata atttgtcatc ttcttcatta actttataag ggttaataat
     3721 tgaagcgatg ataaaaccac caaataagtt taagacaaca gccgttacaa catatttagg
     3781 ttcaattaag gtaaagtatg caccgataat tgaagcagaa acagtcgaca ttgctgaagc
     3841 tgttaatgtg tataaacgtt gcttaggtat gtatggtaat tgttttttaa ttgaaataaa
     3901 tacttcagat tgtcccaaaa ttgctgcagc aactgcattg tatgattcta aacgtcccat
     3961 accattaatt ttagaaatta agaatcctaa aacattaatg attaaaggta aaatctttgt
     4021 gtattgaagg ataccgataa tcgctgaaat aaatacgata ggtaataata cactgaagaa
     4081 gaatggtggt tgcttaggat cgatatattg aataccaccg aatacaaagt taacaccatc
     4141 tgcagctttt aataataagt agttaaaacc gttcgaaata ccaccaataa ccttgattcc
     4201 cattgtagtt ttaagcaaga taaatgcaaa gataagctga attgcaagta aaattcctac
     4261 atatttccag cgaatatttt tcctgtctga gctaaataga aacgcaagtg ctaaaaagaa
     4321 gataattccg ataatcccaa ttagaatatg catatatttc tcattccttt agttttttct
     4381 acaatctatc atacaataaa atggaagggc taacatcata aatttttgaa aatataaaaa
     4441 caaattaatt gaaaaaggtc aaaataggtc atataatata gtcaaagaag gtcaaaaagg
     4501 ggtgatatac atgcacaata tgtctgacat catagaacaa tacatcaaac gtttatttga
     4561 agagtcgaat gaggatgtcg ttgaaattca gagagcgaat atcgcacagc gttttgattg
     4621 cgtaccatca cagttaaact atgtaatcaa aacacgattc actaatgaac atggttatga
     4681 aatcgaaagt aaacgtggtg gtggtggtta catccgaatc actaaaattg aaaataaaga
     4741 tgcaacaggt tatattaatc atttgcttca gctgattgga ccttctattt ctcaacaaca
     4801 agcttattat attattgatg ggcttttaga taaaatgtta ataaatgaac gtgaagctaa
     4861 aatgattcaa gcagttattg atagagaaac gctatcaatg gatatggttt ctagagatat
     4921 tattagagca aatattttaa aacgtttgtt accagttata aattattact aaatgaaatg
     4981 aggtgttgaa gtgctttgtg aaaattgtca acttaatgaa gcggaattaa aagttaaagt
     5041 tacaagtaaa aataaaacag aagaaaaaat ggtgtgtcaa acttgtgctg aggggcacca
     5101 tccgtggaat caagctaatg aacaacctga atatcaagaa catcaagata atttcgaaga
     5161 agcatttgtt gttaagcaaa ttttacaaca tttagctacg aaacatggca tcaattttca
     5221 agaagtagcg tttaaagaag aaaaacgttg cccatcatgt cacatgactt tgaaagatat
     5281 tgcacatgtt ggtaaatttg ggtgtgctaa ttgttatgca acatttaaag atgacatcat
     5341 tgatatcgtc cgcagagttc aaggtggaca atttgagcac gttggaaaga caccacattc
     5401 ttcacataaa aagatagctt taaagcgaaa aatcgaagaa aagaatgaat atttgaaaaa
     5461 acttgttgaa atccaagatt ttgaggaagc agccattgtt agagatgaaa ttaaagcact
     5521 aaaagctgag agtgaggtgc aacatgatga cgcataatat tcatgataat atcagccaat
     5581 ggatgaaaag taatgaagaa acaccaattg ttatgtcttc tagaattcgg ttagcgcgta
     5641 atttagaaaa tcatgtgcat ccactaatgt atgctactga aaatgatgga tttagagtta
     5701 taaatgaggt acaagatgcc ttgccaaatt ttgaattaat gcgtcttgat caaatggatc
     5761 aacaaagtaa aatgaaaatg gttgcaaagc atttgattag ccctgaacta ataaaacaac
     5821 cagcagccgc agtattagtg aatgatgatg aatctttaag tgtcatgata aatgaagagg
     5881 accatattcg tattcaagct atgggaactg acacgacatt acaggcttta tataatcaag
     5941 cttcatcaat tgatgatgaa ttagatcgaa gccttgatat aagttatgat gaacaacttg
     6001 gttatttaac tacatgtcct accaatatag gtactggtat gagagcaagc gtgatgctac
     6061 atttaccagg tctatctatt atgaaaagaa tgacacggat tgctcaaacc attaatcgtt
     6121 ttggatatac aatcagaggt atttacggtg aaggttcgca agtttatgga catacttatc
     6181 aagtatccaa ccaacttaca cttggtaaat ctgagttaga aatcatagaa acattaacag
     6241 aagttgttaa tcaaatcatt catgaagaaa aacaaatacg acaaaagtta gacacttata
     6301 atcaactaga aacacaagac cgtgtttttc gctcgctagg tattttacaa aactgtagaa
     6361 tgataactat ggaagaggct tcttatagat taagcgaagt taaacttggt atagatttaa
     6421 attacattga attacaaaac tttaaattta atgaattgat ggtagctata cagtcaccat
     6481 ttttattaga tgaagaagat gacaaatctg taaaagaaaa acgagcagat atactaagag
     6541 aacatataaa gtaggaggtc attatttatg ttatttggta gattaactga acgtgcacag
     6601 cgcgtattag cacatgcaca agaagaagca attcgtttaa atcattcaaa tataggaaca
     6661 gaacacctat tattggggtt aatgaaagaa cctgaaggaa ttgctgcaaa agtattagaa
     6721 agttttaata tcactgaaga taaagtaatt gaagaagttg aaaaattaat cggacatggt
     6781 caagatcatg ttggtacatt gcattataca cctagagcta aaaaagtcat tgaattatcg
     6841 atggatgaag ctagaaaatt acatcacaat tttgttggaa cggaacatat tttattaggc
     6901 ttgattcgtg agaatgaagg tgttgcagca agagtttttg caaatctaga tttaaatatt
     6961 actaaagcac gtgcacaagt tgtgaaagct ttaggaaacc ctgaaatgag taataaaaat
     7021 gcacaagcta gtaagtcaaa taatactcca actttagata gtttagctcg tgacttaaca
     7081 gtcattgcca aagacggtac attagatcct gttataggac gtgataaaga aattacacgt
     7141 gtaattgaag tattaagtag acgtacgaaa aacaatcctg tgcttattgg agagccaggt
     7201 gttggtaaaa ctgctattgc tgaaggttta gcgcaagcca tagtgaataa tgaggtacca
     7261 gagacattaa aagataagcg tgttatgtct ttagatatgg gaacagtagt tgcaggtact
     7321 aaatatcgtg gtgaatttga agagcgtctg aaaaaggtta tggaagaaat ccaacaagca
     7381 ggtaatgtca tcctatttat tgatgaattg catactttag ttggtgctgg tggtgctgaa
     7441 ggtgctatcg atgcttcgaa tattttgaaa ccggcattag cacgtggtga attacaatgt
     7501 attggtgcta ctacattaga tgaatatcgc aaaaatattg aaaaagacgc ggctttagaa
     7561 cgtcgtttcc aacctgtaca agttgatgaa ccttcagtag tagatacagt tgctatttta
     7621 aaaggattaa gagatcgtta cgaagcacac catcgtatta atatttcaga cgaagctatt
     7681 gaagcagctg ttaaattaag taacagatat gtttcagatc gtttcttacc agataaagca
     7741 attgatttaa ttgatgaagc aagttctaaa gtaagactta agagtcatac gacacctaat
     7801 aatttaaaag aaattgaaca agaaattgaa aaagttaaaa atgaaaaaga tgccgcagta
     7861 catgctcaag agtttgaaaa tgctgctaat ctgcgtgata aacaaacaaa acttgaaaag
     7921 caatatgaag aagctaaaaa tgaatggaag aacgcacaaa atggcatgtc aacttcattg
     7981 tcagaagaag atattgctga agtcattgca ggatggacag gtatcccatt aactaaaatc
     8041 aacgaaacag aatctgaaaa acttcttagt ctagaagata cattacatga gagagttatt
     8101 gggcaaaaag atgctgttaa ttcaatcagt aaagcggtta gacgtgcccg tgcagggtta
     8161 aaagatccta aacgaccaat tggtagcttt atcttccttg gaccaactgg tgttggtaaa
     8221 actgaattag ctagagcttt agctgaatca atgtttggcg atgatgatgc gatgatccgt
     8281 gtagacatga gtgaatttat ggaaaaacac gcagtgagcc gattagttgg tgctcctcca
     8341 ggatatgttg gtcatgatga tggtggacaa ttaactgaaa aagttagacg taaaccatat
     8401 tctgtaattt tatttgatga aattgaaaaa gcacatccag atgtatttaa tattctatta
     8461 caagttttag atgacggaca tttgacagat acaaaaggac gtacagttga tttcagaaat
     8521 acaattatca taatgacatc aaacgttgga gcacaagaat tacaagatca acgatttgct
     8581 ggattcggtg gttcaagtga tggacaagat tatgaaacaa ttcgaaaaac gatgttaaaa
     8641 gaattaaaaa attcattccg tccagaattt ttaaaccgtg tagatgatat cattgtattc
     8701 cataaactaa caaaagaaga attaaaagaa attgtaacta tgatggttaa taaattaaca
     8761 aatcgattat ccgaacaaaa cataaatatt attgttactg ataaagcgaa agataaaatc
     8821 gcagaagaag gatatgatcc agaatatggt gcaagaccat taattagagc gatacaaaaa
     8881 actatcgaag ataatttaag tgaattaata ttagatggta atcaaattga aggtaagaaa
     8941 gttacagtag atcatgatgg taaagagttt aaatatgaca ttgctgaaca aacttcagaa
     9001 actaaaacac catcgcaagc ataattataa aacagtccaa aacaaattaa agttttgggc
     9061 tgttttttag tagcattgaa ctatagaaat tcgtgaaagt atccaccaac gaaacaacct
     9121 aataaaataa tcatcaaagg atagttaaga attatatgaa acaagttaat gagcatacag
     9181 cgatagtaaa aaggtatgaa tttttataaa ggttttttgt ttgaagatac tagcaactta
     9241 cgtcaaaata aaataggtct atataatatt gtgataaatc tagagaaaag agtttactaa
     9301 gaaattatta agattttatc tttgaaagat acctactatc aacatgatgt aagtttattt
     9361 tataatattc ataaaaaata aatatgataa aacagtttgc gttagtagtc atgttaaaat
     9421 acaaacaaat ttaactcaca tttaatttgt cataatggga atgtgcgttt aaaatagatt
     9481 tgttcaaagg aaagtggagg tgcaattttg gccaagaaaa aagtgatttt tgaatgtatg
     9541 gcttgtggtt atcaatctcc taaatggatg gggaaatgtc ctaattgtgg cgcttggaat
     9601 caaatggagg aaattgttga aaaagcagcc aatcctaaac atggagtgaa aaccaaggaa
     9661 ttagtaggta aagtacaaaa attaaatagt attaaacatg aaacaacgcc gagagtgtta
     9721 acagattcag cagaatttaa ccgtgtatta ggtggaggta ttgtgagcgg atcgttagta
     9781 cttattggtg gggatccagg tattggtaag tcaacgttac ttttacaaat ttgtgcatcg
     9841 ttatctcaaa agaaaaaagt actatatatt actggagaag aatcgcttag tcagactaaa
     9901 ttacgtgcag agcgattaga tgaagattca agtgaattgc aagtattagc tgaaacagat
     9961 cttgaagtta tttatcaaac agtaaaagag gaacaacctg atttactagt agtggattcg
    10021 attcaaacaa tatatcatcc tgaaatcagc tctgcgccag gttctgtttc acaagttcgt
    10081 gaaagtacac aaagtttaat gaatattgct aaacaaatga acattgcaac ttttatagtg
    10141 ggtcatgtaa cgaaagaagg tcaaattgct ggcccaagat tgctagaaca catggttgat
    10201 actgtacttt attttgaagg cgatgaacac cacgcatatc gaattttgcg agctgttaaa
    10261 aaccgttttg gttcaacgaa tgaaatggga atcttcgaaa tgaagcaaag tggattaaaa
    10321 ggtgtaaata atccatctga aatgttttta gaagaacgtt caacaaatgt tccaggttca
    10381 acaattgttg caaccatgga gggaactaga ccacttttaa tagaagttca agcgctggta
    10441 actccaacga cttttaacaa tccgagacga atggcaacag ggattgatca caatcgatta
    10501 agtttgttga tggctgtttt ggaaaagaaa gaaaattatc tattacaaca acaagatgct
    10561 tatatcaaag tagctggcgg tgtaaagtta acggagccag cagttgattt aagtgtaatt
    10621 gtagcaactg cgtctagctt taaagataaa gctgtcgacg gattagattg ctatattgga
    10681 gaagttggtt taacaggtga ggtacgtcgt gtatctcgga tagaacaacg cgtgcaagag
    10741 gctgcaaaac taggtttcaa acgtgtaatt attcctaaaa ataatatagg cggatggaca
    10801 tatcctgaag gtatacaagt aataggtgta actactgtac atgaagcatt gtcgtttgct
    10861 cttcattcat aaaacatcaa gaaaggagga cattgtgtga atatcgttaa actaatggtt
    10921 attattattt acttaattat tgggagcgca ttaggaataa ttattattcc tgaaattgca
    10981 aatgatcttg gattacaaaa ctccagcttt ttaaaaaatc actatgtaga tggcattatc
    11041 ggtagtattt ttatgttctt aatttttggt gtatttatta gacgagttac taacgctata
    11101 ataggtttag aacattttat tatgcgtaga agtgctgttg aaatactatt cgcaacaata
    11161 ggtttaataa tcggattact tatttctgtt atggtgtcgt ttatattgga atcaattggc
    11221 aactctattt ttaatcattt cattcctgtc ataattacga tattactatg ttatttcggt
    11281 ttccaatttg gccttaaaaa acgagatgaa atgttaatgt ttttacctga gaatatagcg
    11341 cgttccatgt cacaacatac taaaagtgct acaccaaaaa ttatcgacac aagcgcaatt
    11401 attgatggtc gtattttaga agtcattcgt tgcggtttta tcgatggcaa tattttaatt
    11461 ccacaaggtg ttattaatga attacaaatt gttgcagatt caaatgacag tgttaaacgt
    11521 gaaaagggta aaagaggctt agatatctta aatgaattgt atgatttaga ctatcctaca
    11581 aaggttatac atccaactaa aacacatagt gatattgata caatgctatt aaaactagca
    11641 aaacaatatc atgcaagtat tattacaaca gatttcaacc taaataaagt atgtcatgta
    11701 catggtatca aagcattaaa tgttaatgat ttatcagaag caatcaaacc taatgtacat
    11761 caaggtgatc aacttcatat tttactgaca aaaatgggta aagagcctgg tcaagcagta
    11821 ggatatctag atgatggtac gatggtagtt gttgataatg ctaaaaatct tattggcagt
    11881 catgtcaatt tagaagtagt gagcttattg caaacatctt caggaagaat tgtttttgct
    11941 aaaaaaatcg aagatacagt atcattataa aaactgtagt tgacggaaac aaaatagata
    12001 ttaaaaggta tatgaaatgg cgaagtttgt aatttgaagt ataactataa ataaatacaa
    12061 aacatatcag ttatcaaaaa atacaaatag tgctaacata tcattgaaac aagttcatta
    12121 agtgttaaat tttagatatt aataaagtta ttcttatagc aatcaagaca tacaaaatta
    12181 gaatcaattt taacgtattt gaatagttaa tttgaagttt aattttacag tacataatat
    12241 tcatttttaa gtcgttgaaa tgcgtatgta gtgcagtctt gattcttagg gatagctttt
    12301 ttaaagtgtt gaaaaaacaa ctgctttcta tttaaaattt aagcatatta cccagggaat
    12361 tatgttatga ttaaacaatg aaattatttt aaggtcatgt taaaaagcat tgtttcgttt
    12421 taacaataag acgagttaca catttgttat gacggtcgaa gatagcaaat gaacaattta
    12481 tatgaactaa cttgaaaatc tgatatttaa tttaggagtg aaaaaaatga gcgatcgtat
    12541 aagagtaaga tatgcaccaa gtccaactgg ttatcttcat attggtaatg caagaacagc
    12601 attattcaat tacttgtatg ctaaacatta caacggagat tttgtgattc gaattgaaga
    12661 tactgataaa aaacgtaatt tagaagatgg agaaacatca caatttgata atcttaaatg
    12721 gttaggatta gattgggatg agtctgtaga taaagacaat ggctacggac catatcgtca
    12781 atctgaacgt caacatatct accaaccatt aatagatcag ttactagcag aagataaagc
    12841 atataaatgc tatatgacag aagaagaatt agaagctgaa cgtgaagcgc aaatcgctcg
    12901 tggtgaaatg cctcgctatg gtggtcaaca tgcgcatttg actgaagaac aacgtcaaca
    12961 atttgaagca gaaggacgcc aaccatcaat tcgtttccga gtacctcaaa atcaaacgta
    13021 ttcatttgat gatatggtta aagggaatat ttcatttgat tcaaatggta ttggtgactg
    13081 ggttatcgtt aaaaaagatg gcattccaac gtacaatttt gcagtagcta tagatgatca
    13141 ttacatggaa atttcagatg tcattcgtgg tgatgatcat atttcaaaca cgcctaaaca
    13201 aattatgatt tatgaagcat ttggctggga gccacctcgt tttggtcata tgtcattaat
    13261 tgttaatgaa gaacgtaaaa agttaagtaa acgtgatggg caaattttac aatttattga
    13321 gcaatatcgt gacttaggtt atttacctga agcgttattt aattttattg cgttattagg
    13381 ttggtctcct gaaggtgaag aagaaatctt ttctaaagaa gaatttatca aaatctttga
    13441 tgaaaagcgt ttatcaaaat caccagcatt tttcgataag caaaaattag catgggttaa
    13501 taaccaatat atgaaacaaa aagatactga aacagtattc caattagcat tacctcattt
    13561 aattaaagcg aatttgattc ctgaggtgcc gtcagaagag gatttatctt ggggacgcaa
    13621 attaattgcg ctttatcaaa aagaaatgag ttatgccggt gaaattgtac ctttatcaga
    13681 aatgttcttt aaagaaatgc cagctcttgg tgaggaagaa caacaagtga tcaatggaga
    13741 gcaagtacca gagttaatga cgcacttatt cagtaaatta gaagcacttg aaccatttga
    13801 agcggctgaa attaaaaaga caattaaaga agttcaaaaa gaaacaggaa taaaaggcaa
    13861 gcaattattt atgcctattc gtgttgctgt aacaggccaa atgcatggtc ctgaattacc
    13921 aaatacaatt gaagtacttg gtaaagaaaa agtgctaaac cgtttaaaac aatataagta
    13981 atgaaataga caagatcaag gtagtatata cttgaatttt ataacgtaac cacatatgat
    14041 aaagttgtat taacaaatta caacaatatg aatagagtac tttatataaa acgaaggatt
    14101 agtaattaaa tttatacgat gcagagagtg tacggttgct gtgagtacaa cgtagaaatt
    14161 aatgaatgca ccttcgtaaa atgaattaaa tatataatga gagtgatgag cattaagttg
    14221 acttagtttc cttgataatt tggaagcgcc cgcaatttta ttaatgtaat tcgctaaatt
    14281 cagagtggaa ccgtgcggaa gcgcctctaa caatacaatt tgtatgttag tggtgctttt
    14341 tttgatattt aatttcgcag gtacttcaat tatgtattta cgtagttcaa tcattgagga
    14401 ggaaatgatc ttgttaaaaa gaatgagaga cgatataaaa atggtatttg agcaggatcc
    14461 agcggcacgt tcaacattag aagtcattac aacgtatgca ggtttacatg cagtttggag
    14521 tcatttgatt gcacataagt tatacaacca aaaaaaatat gttgcagcac gcgcgatatc
    14581 tcaaatttca agatttttca caggtataga aatccatcca ggtgctaaaa ttggaaagcg
    14641 tctatttata gatcatggta tgggcgttgt aataggagaa acatgtacaa ttggtgataa
    14701 tgtgacaatc tatcaaggcg tgacacttgg tgggacaggg aaagaaagag ggaaaagaca
    14761 cccagatata ggagacaatg ttttaatagc agcgggtgcg aaagttttag gaaatattaa
    14821 aataaattca aatgtaaata ttggtgcaaa ttcagttgtt ttacaatcag ttccaagtta
    14881 ttcaacggtt gttggtatac caggacatat tgttaagcaa gatggtgttc gagttggaaa
    14941 aacatttgat catcgccatc tacctgatcc aatttatgaa caaattaagc atttagaacg
    15001 acaacttgaa aagactagga atggagagat tcaagatgat tacattatat aatacgctta
    15061 cacgtcaaaa agaagtgttc aagccaatag aaccaggaaa ggtaaaaatg tatgtatgtg
    15121 gtcctacggt atataactac attcatattg gtaatgcaag accagcaatt aattatgacg
    15181 tagtgagacg ttactttgaa taccaaggat ataatgtaga atatgtatca aattttacag
    15241 atgtagatga taaattaatt aaacgttctc aaggattaaa tcagactgtt cccgaaattg
    15301 cagaaaaata tatcgctgct tttcatgaag atgttggtgc tttaaatgtt agaaaagcga
    15361 cttcaaatcc aagggtaatg gaccatatgg atgacattat tcaatttatt aaagatttgg
    15421 ttgatcaagg ttatgcatat gaaagtggtg gcgatgttta ctttagaaca cgtaaatttg
    15481 aaggttatgg taaattaagt catcaatcca tagatgactt aaaagtgggt gctcgtatag
    15541 atgcaggaga gcataaggaa gacgcacttg attttacatt gtggaaaaaa gcgaagcctg
    15601 gcgagattag ttgggatagc ccatttggtg aagggagacc aggatggcat attgaatgtt
    15661 ctgtaatggc atttcatgag ctaggagcta caattgatat acatgcgggt ggttcagatt
    15721 tacaatttcc acatcatgaa aatgaaatag cacaatcgga agcacataat catgcgccat
    15781 tcgctaatta ttggatgcat aatggtttta ttaatattga taatgaaaaa atgagtaaat
    15841 cactaggcaa ctttatttta gttcacgata ttattaaaga agttgatcca gatgtattaa
    15901 gattctttat gattagcgta cattatagaa gtccaattaa ctataatcta gaattggttg
    15961 aatcagcacg tagtggacta gagcgtattc gcaatagtta tcaattaatt gaagagcgcg
    16021 cacaaattgc tactaatatt gaaaatcaac agacatatat tgatcaaatt gatgcgattt
    16081 taaatcgttt tgaaacagtt atgaatgatg attttaatac agctaatgca attacagctt
    16141 ggtatgattt agcaaaactt gcaaataaat atgtactaga gaacacaaca tcaacagaag
    16201 taattgataa atttaaagca gtttatcaaa ttttcagcga tgttttaggt gtaccgttaa
    16261 aatctaaaaa agcagatgaa ttattagatg aagatgttga aaaattaatc gaagagcgta
    16321 atgaagcaag gaaaaacaaa gattttgcac gagcagatga gattcgagac atgctgaaat
    16381 cacaaaacat tatattagaa gacacacctc aaggggttag atttaaacgt ggataatcaa
    16441 caagataatc acattaaatt attgaatcca ttgaccttag catatatggg agacgcagtc
    16501 ttagatcaat atgtacgtac gtatatcgtt ttaaagctta aaagtaagcc taataaacta
    16561 catcaaatgt ctaaaaaata tgtatctgcc aaaagtcagg cgcaaacgtt agaatattta
    16621 atggagcaag aatggtttac agacgaagaa atggatattt tgaaacgagg gcgtaacgcg
    16681 aaaagtcata ctaaagctaa aaatactgat gttcaaacat atcgtaaaag ttcagcgata
    16741 gaagcagtga taggttttct ttatttagaa aaaagagaag aacgattaga ggcattatta
    16801 aatgaaataa taacaatagt aaacgaaagg tagtgacaat gtggaagata cggttattgt
    16861 tggtaggcat gctgttagag aagcgattat tactgggcat ccgataaata agatattgat
    16921 tcaagaagga attaaaaagc aacaaattaa tgaaatttta aaaaatgcaa aagatcaaaa
    16981 aatcattgtt caaactgtac caaaatctaa attagatttt ttagcaaatg caccacatca
    17041 gggggttgca gcgcttattg caccatatga atatgctgac ttcgatcaat ttttaaaaca
    17101 gcaaaaagaa aaagaaggtt tatcgacagt gcttatatta gacggcttag aagacccgca
    17161 taacttggga tcaattttaa gaacagcaga tgcaacggga gttgatggtg ttattattcc
    17221 taaacgtcgt tcagttacac taacgcaaac agttgcaaaa gcctcaacag gtgcaattga
    17281 acatgtacca gttattcgag tgacaaattt agctaaaact atcgatgaac taaaagataa
    17341 tggtttttgg gtagctggca ctgaagctaa taatgcaaca gattatagaa atcttgaagc
    17401 ggacatgtca ttggctattg taattggcag cgaaggacag ggtatgagtc gtctagtaag
    17461 tgataaatgc gatttttata ttaagattcc aatggttggc catgtaaaca gtttgaatgc
    17521 ttcggttgca gcaagtttaa tgatgtacga agtatttcga aaaagacatg atgttggaga
    17581 aatataatga aagaacgtta cttaatcatt gatggataca atatgatagg acaatcacca
    17641 acgctaagcg ccattgcaaa agagaattta gaagaagcta gaatgcaatt aatagataca
    17701 attgcaaatt ataatgcagt tatttcagat gaaattattt gtgttttcga tgcttatgac
    17761 caatcgggtg ttgaaagaga atacatgtat catggcgtta aaacaatttt taccaaggaa
    17821 aaagaaacag ctgatagttt catagaacgt tatgtctatg aactttatga caagcacact
    17881 aagcatatta cagttgtaac aagtgatatg agtgagcaac atgctatctt tggatcaggt
    17941 gcatatagaa tatcatctcg cgaaatgtgg agagatttaa aagaaaatga aattgatgtg
    18001 agtaaatcat tagatgatat aagtgaaaac aagccaagaa ctcgaattcc gttatcttct
    18061 gaaatccttg cagaatttga aaaaatacga agaggacatc ataagaaatg acatttccat
    18121 catcttgaaa ttttgaatgt aacaatatta aactaacctt aaattttaga tagaaggggt
    18181 tagtttaata cttgaaatat gatttgacaa ctcaagacag tacaatcaaa cgtaacaatg
    18241 caataaatga taaagacttc gaaaagttag taatggatct acaaccatta attatacggc
    18301 gcatcaaaac atttggattt aatcattatg atttagaaga tttatatcaa gaaatactta
    18361 tacggatgta taggtcggtc caaacatttg attttagtgc agagcagcct ttcacaaatt
    18421 atgttcaatg tttaattacg tctgtaaagt atgattattt gagaaaatat ttagctacaa
    18481 ataaaagaat ggataatttg attaatgaat atagagttac gtatccatgt gcaataaagc
    18541 gttatgatgt tgaaaacaat tatttgaata aattagcaat taaagagttg attcgtcagt
    18601 ttaagtattt gagtgcattt gaaaaagatg tcatgtattt aatgtgtgaa caatataagc
    18661 cgagagaaat tgctcaactg atgcatgtaa aagagaaagt gatttataat gccatacaac
    18721 gatgtaaaaa taaaataaaa cgttatttca aaatgatttg aaaagcgtct taggacgtga
    18781 attgaattag aacgtgttac ttcctgatgg tttgacattt gttataaatt ttatgtatag
    18841 tatactggta ttataatgaa taaaggtgaa ttattgtgag gaaaatacct ttaaattgtg
    18901 aagcttgtgg caatagaaat tataatgttc ctaagcaaga aggcacggca acaagattaa
    18961 ccttaaagaa atattgtcca aaatgtaacg cgcacacaat tcataaagaa tcgaaataaa
    19021 tacattcgaa ataatacttt gataatatgt tcaaaggatt tggaggttga gcagatggct
    19081 aaaaaagaaa gtttctttaa aggcgttaag tctgaaatgg aaaaaacaag ttggccgacg
    19141 aaagaagagc tatttaaata tactgtaatt gtagtttcta ctgttatatt cttcttagtc
    19201 tttttctatg ccttagattt aggaattaca gcattgaaaa atttattatt tggttagagg
    19261 agtgaagaca tgtctgaaga agttggcgca aagcgttggt atgcagtgca tacatattct
    19321 ggatatgaaa ataaagttaa aaagaattta gaaaaaagag tagaatctat gaatatgact
    19381 gaacaaatct ttagagtagt cataccggaa gaagaagaaa ctcaagtaaa agatggcaaa
    19441 gctaaaacga ctgttaaaaa aacattccct ggatatgttt tagtggaatt aatcatgaca
    19501 gatgaatcat ggtatgtggt aagaaataca ccaggcgtta ctggttttgt aggttctgca
    19561 ggtgcagggt ctaagccaaa tccattgtta ccagaagaag ttcgcttcat cttaaaacaa
    19621 atgggtctta aagaaaagac tatcgatgtt gaactcgaag ttggcgagca agttcgtatt
    19681 aaatcaggtc catttgcgaa tcaagttggt gaagttcaag aaattgaaac agataagttt
    19741 aaactaacag tattagtaga tatgtttggc cgagaaacac cagtagaagt tgaattcgat
    19801 caaatagaaa agctttaatt aacaattaaa gttattaaac taaccaaaag ataaaaaaga
    19861 gtattgattt tttaattaga aaagtgttaa aattatgtgg tcgcgctttt agagcgccca
    19921 tttcgtcacg aaatgttaag agtgggaggg caaaactgag ccctgtgacc acatcacgat
    19981 atcaaggagg tgcacatcgt ggctaaaaaa gtagataaag ttgttaaatt acaaattcct
    20041 gcaggtaaag cgaatccagc accaccagtt ggtccagcat taggtcaagc aggtgtgaac
    20101 atcatgggat tctgtaaaga gttcaatgca cgtactcaag atcaagcagg tttaattatt
    20161 ccggtagaaa tcagtgttta tgaagatcgt tcatttacat ttattacaaa aactccaccg
    20221 gctccagtat tacttaaaaa agcagctggt attgaaaaag gttcaggtga accaaacaaa
    20281 actaaagttg ctacagtaac taaagatcaa gtacgcgaaa ttgctaacag caaaatgcaa
    20341 gacttaaacg ctgctgacga agaagcagct atgcgtatta tcgaaggtac tgcacgtagt
    20401 atgggtatcg ttgtagaata attttacgaa tattaaattt gattacatga tttaaacgat
    20461 gaagcagata acagagataa taatgatgaa ttataaatat aatctgaatg actagattaa
    20521 tgattgattt attcatatga ttaattcttc tgttgtctgc tcttaacttg catctagcaa
    20581 gtaatgtggg aggaaattcc gctaaaacca ctaaaggagg aactataaat ggctaaaaaa
    20641 ggtaaaaagt atcaagaagc agctagtaaa gttgaccgta ctcagcacta cagtgttgaa
    20701 gaagcaatta aattagctaa agaaacaagc attgctaact ttgacgcttc tgttgaagtt
    20761 gcattccgtt taggaattga tacacgtaaa aatgaccaac aaatccgtgg tgcagttgta
    20821 ttaccaaacg gaactggtaa atcacaaagt gtattagtat tcgctaaagg tgacaaaatt
    20881 gctgaagctg aagcagcagg tgctgactat gtaggtgaag cagaatacgt tcaaaaaatc
    20941 caacaaggtt ggttcgactt cgatgtagta gttgctacac cagacatgat gggtgaagtt
    21001 ggtaaattag gtcgtgtatt aggaccaaaa ggtttaatgc caaaccctaa aactggaact
    21061 gtaacaatgg atgttaaaaa agctgttgaa gaaatcaaag ctggtaaagt agaataccgt
    21121 gctgaaaaag ctggtatcgt acatgcatca attggtaaag tttcatttac tgatgaacaa
    21181 ttaattgaaa acttcaatac tttacaagat gtattagcta aagctaaacc atcatctgct
    21241 aaaggtacat acttcaaatc tgttgctgta actacaacaa tgggtcctgg agttaaaatt
    21301 gatactgcaa gtttataata ataaatgata taaacaatta caggctgaaa gaaatatctt
    21361 tcagtctgta aaaatatatt gacaataagt aatttccaag ttatattact tattgtgatt
    21421 attttaccta agacagtagg agttatttat aacttaaaat ttatcctgcc gaggctaaaa
    21481 ttgacttgaa cgtgatgatc tatgatcttt caagcacttt ttgccgtggg tagaaagtgc
    21541 tttttttatt aattttaaaa aaagcaccaa aaatttaaat ggaggtgtct gaatgtctgc
    21601 tatcattgaa gctaaaaaac aactagttga tgaaattgct gaggtactat caaattcagt
    21661 ttcaacagta atcgttgact accgtggatt aacagtagct gaagttactg acttacgttc
    21721 acaattacgt gaagctggtg ttgagtataa agtatacaaa aacactatgg tacgtcgtgc
    21781 agctgaaaaa gctggtatcg aaggcttaga tgaattctta acaggtccta ctgctattgc
    21841 aacttcaagt gaagatgctg tagctgcagc gaaagtaatt tctggatttg ctaaagatca
    21901 tgaagcatta gaaattaaat caggcgttat ggaaggcaat gttattacag cagaagaagt
    21961 taaaactgtt ggttcattac cttcacacga tggtcttgta tctatgcttt tatcagtatt
    22021 acaagctcct gtacgcaact tcgcttatgc ggttaaagct attggagaac aaaaagaaga
    22081 aaacgctgaa taatttttag cgtaaaaaaa ttaaaaataa tggaggaatt ataaaatggc
    22141 taatcatgaa caaatcattg aaacgattaa agaaatgtca gtattagaat taaacgactt
    22201 agtaaaagca attgaagaag aatttggtgt aactgcagct gctccagtag cagtagcagg
    22261 tgcagctggt ggcgctgacg ctgcagcaga aaaaactgaa tttgacgttg agttaacttc
    22321 agctggttca tctaaaatca aagttgttaa agctgttaaa gaagcaactg gtttaggatt
    22381 aaaagatgct aaagaattag tagacggagc tcctaaagta atcaaagaag ctttacctaa
    22441 agaagaagct gaaaaactta aagaacaatt agaagaagtt ggagctactg tagaattaaa
    22501 ataattcaag tatcttaaac ttaataatca aagttttata gcaagtattg ctataatata
    22561 atgattcttt gagaagttaa aaccccgtta ttttgataac ggggttttat tcatttaaag
    22621 actaagtgaa atgttataat tataatgacg agttacaaag tgaagatgag gtggaataat
    22681 gagtcattat tacgatgaag atccaagtgt aattagcaat gaacaacgta ttcaatatca
    22741 attaaaccat cataaaattg atttaataac tgataacgga gtgttttcga aagataaagt
    22801 agattatggt tcagatgttc ttgttcaaac ttttttaaaa gcgcatccac ctggtccaag
    22861 taagcgaatt gccgatgttg gttgtggtta cggaccaatt ggtttgatga ttgctaaagt
    22921 atcaccacat cattcaatta caatgctaga tgttaatcac agagcgctag ccttagttga
    22981 aaaaaacaaa aaattaaatg gtattgataa tgtgatcgta aaggaaagtg atgctttgtc
    23041 tgctgtggaa gacaaaagtt ttgattttat tttaaccaat ccaccaataa gagcagggaa
    23101 agaaaccgtg catcgtatat tcgagcaagc attacataga ttagactcga acggtgaact
    23161 atttgttgta attcagaaga agcaaggtat gccatctgca aagaaaagaa tgaatgaact
    23221 ttttggaaat gtagaagtgg taaataaaga taaaggatat tacattctga gaagtataaa
    23281 agcttgaaat gaaatggata ttctgttata gttatttaat gtaaaaattt atgttcaata
    23341 agtgtgtact tttacgttaa atagataagt taattaagaa taaatataga atcgaaaatg
    23401 gtgtcatcat tagtgttgcc gttttctttt tgtcttttta ttaatatgct tatggtattt
    23461 agctaaaagc ggatcacata atttttgagg ggtgaatctg tttggcaggt caagttgtcc
    23521 aatatggaag acatcgtaaa cgtagaaact acgcgagaat ttcagaagta ttagaattac
    23581 caaacttaat agaaattcaa actaaatctt acgagtggtt cctaagagaa ggtttaatcg
    23641 aaatgtttag agacatttct ccaattgaag attttactgg taatttgtca ttagagtttg
    23701 tggattaccg tttaggagaa ccaaaatatg atttagaaga atctaaaaac cgtgacgcta
    23761 cttatgctgc acctcttcgt gtaaaagtgc gtctaatcat taaagaaaca ggagaagtta
    23821 aagaacaaga agtctttatg ggtgatttcc cattaatgac tgatacaggt acgttcgtta
    23881 tcaatggtgc agaacgtgta atcgtatctc aattagttcg ttcaccatcc gtttatttca
    23941 atgaaaaaat cgacaaaaat ggtcgtgaaa actatgatgc aacaattatt ccaaaccgag
    24001 gtgcatggtt agaatatgaa acagatgcta aagatgttgt atacgtgcgt attgatagaa
    24061 cacgtaaact accattaaca gtattgttac gtgcattagg tttctcaagt gaccaagaaa
    24121 ttgttgacct tttaggtgac aatgaatatt tacgtaatac tttagagaaa gacggcactg
    24181 aaaacactga acaagcgtta ttagaaatct atgaacgttt acgtccaggt gaaccaccaa
    24241 ctgttgaaaa tgctaaaagt ctattgtatt cacgtttctt tgatccaaaa cgctatgact
    24301 tagcaagcgt gggtcgttat aaaacaaaca aaaaattaca tttaaaacat cgtttattca
    24361 atcaaaaatt agctgagcca attgtgaata ctgaaactgg tgaaattgta gttgaagaag
    24421 gtacagtgct tgatcgtcgt aaaatcgacg aaatcatgga tgtacttgaa tcaaacgcaa
    24481 acagcgaagt gtttgaattg catggtagcg ttatagacga gccagtagaa attcaatcaa
    24541 ttaaagtata tgttcctaac gatgatgaag gtcgtacgac aactgtaatt ggtaatgctt
    24601 tccctgactc agaagttaaa tgtattacac cggcagatat catcgcttca atgagttact
    24661 tctttaactt attaagtggt attggatata cagatgatat tgaccattta ggtaaccgtc
    24721 gtttacgttc tgtaggtgaa ttactacaaa accaattccg tatcggttta tcaagaatgg
    24781 aaagagttgt acgtgaaaga atgtcaattc aagatactga gtctatcaca cctcaacaat
    24841 taattaatat tcgacctgtt attgcatcta ttaaagaatt ctttggtagc tctcaattat
    24901 cacaattcat ggaccaagca aatccattag ctgagttaac gcataaacgt cgtctatcag
    24961 cattaggacc tggtggttta acacgtgaac gtgctcaaat ggaagtgcgt gacgttcact
    25021 actctcacta tggccgtatg tgtccaattg aaacgcctga gggaccaaac attggattga
    25081 ttaactcatt atcaagttat gcacgtgtaa atgaattcgg ctttattgaa acaccatatc
    25141 gtaaagttga tttagataca catgctatca ctgatcaaat tgactattta acagctgacg
    25201 aagaagatag ctatgttgta gcacaagcaa actctaaatt agatgaaaat ggtcgtttca
    25261 tggatgatga agttgtatgt cgtttccgtg gtaacaatac agttatggct aaagaaaaaa
    25321 tggattatat ggatgtatcg ccgaagcaag ttgtttcagc agcgacagca tgtattccat
    25381 tcttagaaaa tgatgactca aaccgtgcat tgatgggtgc gaacatgcaa cgtcaagcag
    25441 tgcctttgat gaatccagaa gcaccatttg ttggtacagg tatggaacac gttgcagcac
    25501 gtgattctgg tgcagctatt acagctaagc acagaggtcg tgttgaacat gttgaatcta
    25561 atgaaattct tgtacgtcgt ctagttgaag agaacggcgt tgagcatgaa ggtgaattag
    25621 atcgctatcc attagctaaa tttaaacgtt caaactcagg tacatgttac aaccaacgtc
    25681 caatcgttgc agttggagat gttgttgagt ttaacgagat tttagcagat ggaccatcta
    25741 tggaattagg agaaatggca ttaggtagaa acgtagtagt tggtttcatg acttgggacg
    25801 gttacaacta tgaggatgcc gttatcatga gtgaaagact tgtgaaagat gacgtgtata
    25861 cttctattca tattgaagag tatgaatcag aagcacgtga tactaagtta ggacctgaag
    25921 aaatcacaag agatattcct aatgtttctg aaagtgcact taagaactta gacgatcgtg
    25981 gtatcgttta tattggtgca gaagtaaaag atggagatat tttagttggt aaagtaacgc
    26041 ctaaaggtgt aactgagtta actgccgaag aaagattgtt acatgcaatc tttggtgaaa
    26101 aagcacgtga agttagagat acttcattac gtgtacctca cggcgctggc ggtatcgttc
    26161 ttgatgtaaa agtattcaat cgtgaagaag gcgacgacac attatcacct ggtgtaaacc
    26221 aattagtacg tgtatatatc gttcaaaaac gtaaaattca tgttggtgat aagatgtgtg
    26281 gtcgacatgg taacaaaggt gtcatttcta agattgttcc tgaagaagat atgccttact
    26341 taccagatgg acgtccgatt gatatcatgt taaatcctct tggtgtacca tctcgtatga
    26401 acatcggaca agtattagag ctacacttag gtatggctgc taaaaatctt ggtattcacg
    26461 ttgcatcacc agtatttgac ggtgcaaacg atgacgatgt atggtcaaca attgaagaag
    26521 ctggtatggc tcgtgatggt aaaactgtac tttatgatgg acgtacaggt gaaccattcg
    26581 ataaccgtat ttcagtaggt gtaatgtaca tgttgaaact tgcgcacatg gttgatgata
    26641 aattacatgc gcgttcaaca ggaccatatt cacttgttac acaacaacca cttggcggta
    26701 aagcgcaatt cggtggacaa cgtttcggtg agatggaggt atgggcactt gaagcatatg
    26761 gtgctgcata cacattacaa gaaatcttaa cttacaaatc cgatgataca gtaggacgtg
    26821 tgaaaacata cgaggctatt gttaaaggtg aaaacatctc tagaccaagt gttccagaat
    26881 cattccgagt attgatgaaa gaattacaaa gtttaggttt agatgtaaaa gttatggatg
    26941 agcaagataa tgaaatcgaa atgacagacg ttgatgacga tgatgttgta gaacgcaaag
    27001 tagatttaca acaaaatgat gctcctgaaa cacaaaaaga agttactgat taatacgcaa
    27061 tttacaaaac aggcaaaaag atactaagct gaattttatt gatgattcgg tttagtactt
    27121 taagccattt taaataaatg caaatcaatc aaatagcaca gctaatctaa attgaaggag
    27181 gtaggctcct tgattgatgt aaataatttc cattatatga aaataggatt ggcttcacct
    27241 gaaaaaatcc gttcttggtc ttttggtgaa gttaaaaaac ctgaaacaat caactaccgt
    27301 acattaaaac ctgaaaaaga tggtctattc tgtgaaagaa ttttcggacc tacaaaagac
    27361 tgggaatgta gttgtggtaa atacaaacgt gttcgctaca aaggcatggt ctgtgacaga
    27421 tgtggagttg aagtaactaa atctaaagta cgtcgtgaaa gaatgggtca cattgaactt
    27481 gctgctccag tttctcacat ttggtatttc aaaggtatac caagtcgtat gggattatta
    27541 cttgacatgt caccaagagc attagaagaa gttatttact ttgcttctta tgttgttgta
    27601 gatccaggtc caactggttt agaaaagaaa actttattat ctgaagctga attcagagat
    27661 tattatgata aatacccagg tcaattcgtt gcaaaaatgg gtgcagaagg tattaaagat
    27721 ttacttgaag agattgatct tgacgaagaa cttaaattgt tacgcgatga gttggaatca
    27781 gctactggtc aaagacttac tcgtgcaatt aaacgtttag aagttgttga atcattccgt
    27841 aattcaggta acaaaccttc atggatgatt ttagatgtac ttccaatcat cccaccagaa
    27901 attcgtccaa tggttcaatt agatggtgga cgatttgcaa caagtgactt aaacgactta
    27961 taccgtcgtg taattaatcg aaataatcgt ttgaaacgtt tattagattt aggtgcacct
    28021 ggtatcatcg ttcaaaacga aaaacgtatg ttacaagaag ccgttgacgc tttaattgat
    28081 aatggtcgtc gtggtcgtcc agttactggc ccaggtaacc gtccattaaa atctttatct
    28141 catatgttaa aaggtaaaca aggtcgtttc cgtcaaaacc tacttggtaa acgtgttgac
    28201 tattcaggac gttcagttat cgcggtaggt ccaagcttga aaatgtacca atgtggttta
    28261 ccgaaagaaa tggcacttga actatttaaa ccatttgtaa tgaaagaatt agttcaacgt
    28321 gaaattgcaa ctaacattaa aaatgcgaag agtaaaatcg aacgcatgga tgatgaagtt
    28381 tgggacgtat tggaagaagt aattagagaa catcctgtat tacttaaccg tgcaccaaca
    28441 cttcatagac ttggtattca agcatttgaa ccaactttag ttgaaggtcg tgcgattcgt
    28501 ctacatccac ttgtaacaac agcttataac gctgactttg atggtgacca aatggcggtt
    28561 cacgttcctt tatcaaaaga ggcacaagct gaagcaagaa tgttgatgtt agcagcacaa
    28621 aacatcttga accctaaaga tggtaaacca gtagttacac catcacaaga tatggtactt
    28681 ggtaactatt accttacttt agaaagaaaa gatgcagtaa atacaggcgc aatctttaat
    28741 aatacaaatg aagtgttaaa agcatatgca aatggctttg tacatttaca cacaagaatt
    28801 ggtgtacatg caagttcatt caacaaccca acatttactg aagaacaaaa caaaaagatt
    28861 cttgctacgt cagtaggtaa aattatattc aatgaaatca ttccggattc atttgcttat
    28921 attaatgaac ctacgcaaga aaacttagaa agaaagacac caaacagata cttcatcgat
    28981 cctacaactt taggtgaagg tggattaaaa gaatactttg aaaatgaaga attaattgaa
    29041 cctttcaaca aaaaattctt aggtaatatt attgcagaag tattcaacag atttagcatc
    29101 actgatacat caatgatgtt agaccgtatg aaagacttag gattcaaatt ctcatctaaa
    29161 gctggtatta cagtaggtgt tgctgatatc gtagtattac ctgataagca acaaatactt
    29221 gatgagcatg aaaaattagt cgacagaatt acaaaacaat tcaatcgtgg tttaatcact
    29281 gaagaagaaa gatataatgc agttgttgaa atttggacag atgcaaaaga tcaaattcaa
    29341 ggtgaattga tgcaatcact tgataaaact aacccaatct tcatgatgag tgattcaggt
    29401 gcccgtggta acgcatctaa ctttacacag ttagcaggta tgcgtggatt gatggccgca
    29461 ccatctggta agattatcga attaccaatc acatcttcat tccgtgaagg tttaacagta
    29521 cttgaatact tcatctcaac tcacggtgcg cgtaaaggtc ttgccgatac agcacttaag
    29581 acagctgact caggatatct tactcgtcgt cttgttgacg tggcacaaga tgttattgtt
    29641 cgtgaagaag actgtggtac agatagaggt ttattagttt ctgatattaa agaaggtaca
    29701 gaaatgattg aaccatttat cgaacgtatt gaaggtcgtt attctaaaga aacaattcgt
    29761 catcctgaaa ctgatgaagt tatcattcgt cctgatgaat taattacacc tgaaattgct
    29821 aagaaaatta cagatgctgg tattgaacaa atgtatattc gctcagcatt tacttgtaac
    29881 gcaagacacg gtgtttgtga aaaatgttac ggtaaaaacc ttgctactgg tgaaaaagtt
    29941 gaagttggtg aagcagttgg tacaattgca gcccaatcta tcggtgaacc aggtacacag
    30001 cttacaatgc gtacattcca tacaggtggg gtagcaggta gcgatatcac acaaggtctt
    30061 cctcgtattc aagagatttt cgaagcacgt aaccctaaag gtcaagcggt aattacggaa
    30121 atcgaaggtg tcgtagaaga tattaaatta gcaaaagata gacaacaaga aattgttgtt
    30181 aaaggtgcta atgaaacaag atcatatctt gcttcaggta cttcaagaat tattgtagaa
    30241 atcggtcaac cagtacaacg tggtgaagta ttaactgaag gttctattga acctaagaat
    30301 tacttatctg ttgctggatt aaacgcgact gaaagctact tattaaaaga agtacaaaaa
    30361 gtttaccgta tgcaaggggt agaaatcgac gataaacacg ttgaggttat ggttcgacaa
    30421 atgttacgta aagttagaat tatcgaagca ggtgatacga agttattacc aggttcatta
    30481 gttgatattc acaactttac agatgcaaat agagaagcat ttaaacaccg caagcgccct
    30541 gcaacagcta aaccagtatt acttggtatt actaaagcat cacttgaaac agaaagtttc
    30601 ttatctgcag catcattcca agaaacaaca agagttctaa cagatgcagc aattaaaggt
    30661 aagcgtgatg acttattagg tcttaaagaa aacgtaatta tcggtaagct aattccagct
    30721 ggtactggta tgagacgtta tagcgacgta aaatacgaaa aaacagctaa accagttgca
    30781 gaagttgaat ctcaaactga agtaacggaa taacaagtat ataacagagg ctaaggcttt
    30841 agcctcttgt tatttttatg taaattattt gatttaatgt tgacgaattc tcttgttcaa
    30901 tgttaatata ttaaaggttg atgcaagcag aactttggag gataaattat tgtctaagga
    30961 aaaagttgca cgctttaaca aacaacattt tgtagttggt cttaaagaaa cgcttaaagc
    31021 gttaaagaaa gatcaagtta catctttgat tattgctgaa gacgttgaag tatatttaat
    31081 gactcgcgtg ttaagccaaa tcaatcagaa aaatatacct gtatcttttt tcaaaagcaa
    31141 acatgctttg ggtaaacatg taggtattaa cgtcaatgcg acaatagtag cattgattaa
    31201 atgagaatta gtaagtgttt aacttactaa attttattta acctaaaaat gaaccacctg
    31261 gatgtgtggg attaaaaagt gaagagagga ggacatatca catgccaact attaaccaat
    31321 tagtacgtaa accaagacaa agcaaaatca aaaaatcaga ttctccagct ttaaataaag
    31381 gtttcaacag taaaaagaaa aaatttactg acttaaactc accacaaaaa cgtggtgtat
    31441 gtactcgtgt aggtacaatg acacctaaaa aacctaactc agcgttacgt aaatatgcac
    31501 gtgtgcgttt atcaaacaac atcgaaatta acgcatacat ccctggtatc ggacataact
    31561 tacaagaaca cagtgttgta cttgtacgtg gtggacgtgt aaaagactta ccaggtgtgc
    31621 gttaccatat tgtacgtgga gcacttgata cttcaggtgt tgacggacgt agacaaggtc
    31681 gttcattata cggaactaag aaacctaaaa actaagaatt tagtttttaa ttaaatctta
    31741 aacttaaaat atttaatata aggaagggag gatttacatt atgcctcgta aaggatcagt
    31801 acctaaaaga gacgtattac cagatccaat tcataactct aagttagtaa ctaaattaat
    31861 taacaaaatt atgttagatg gtaaacgtgg aacagcacaa agaattcttt attcagcatt
    31921 cgacctagtt gaacaacgca gtggtcgtga tgcattagaa gtattcgaag aagcaatcaa
    31981 caacattatg ccagtattag aagttaaagc tcgtcgtgta ggtggttcta actatcaagt
    32041 accagtagaa gttcgtccag agcgtcgtac tactttaggt ttacgttggt tagttaacta
    32101 tgcacgtctt cgtggtgaaa aaacgatgga agatcgttta gctaacgaaa ttttagatgc
    32161 agcaaataat acaggtggtg ccgttaagaa acgtgaggac actcacaaaa tggctgaagc
    32221 aaacaaagca tttgctcact accgttggta agataaaagc ttttaccctg agtgtgttct
    32281 atattaatga attttcatta agcgttcatg cttagggcat cgccatatct atcgtattta
    32341 ttcagtaata taaactggaa ggagaaaaaa tacatggcta gagaattttc attagaaaaa
    32401 actcgtaata tcggtatcat ggctcacatt gatgctggta aaacgactac gactgaacgt
    32461 attctttatt acactggccg tatccacaaa attggtgaaa cacatgaagg tgcttcacaa
    32521 atggactgga tggagcaaga acaagaccgt ggtattacta tcacatctgc tgcaacaaca
    32581 gcagcttggg aaggtcaccg tgtaaacatt atcgatacac ctggacacgt agacttcact
    32641 gtagaagttg aacgttcatt acgtgtactt gacggagcag ttacagtact tgatgcacaa
    32701 tcaggtgttg aacctcaaac tgaaacagtt tggcgtcagg ctacaactta tggtgttcca
    32761 cgtatcgtat ttgtaaacaa aatggacaaa ttaggtgcta acttcgaata ctctgtaagt
    32821 acattacatg atcgtttaca agctaacgct gctccaatcc aattaccaat tggtgcggaa
    32881 gacgaattcg aagcaatcat tgacttagtt gaaatgaaat gtttcaaata tacaaatgat
    32941 ttaggtactg aaattgaaga aattgaaatt cctgaagacc acttagatag agctgaagaa
    33001 gctcgtgcta gcttaatcga agcagttgca gaaactagcg acgaattaat ggaaaaatat
    33061 cttggtgacg aagaaatttc agtttctgaa ttaaaagaag ctatccgcca agctactact
    33121 aacgtagaat tctacccagt actttgtggt acagctttca aaaacaaagg tgttcaatta
    33181 atgcttgacg ctgtaattga ttacttacct tcaccactag acgttaaacc aattattggt
    33241 caccgtgcta gcaaccctga agaagaagta atcgcgaaag cagacgattc agctgaattc
    33301 gctgcattag cgttcaaagt tatgactgac ccttatgttg gtaaattaac attcttccgt
    33361 gtgtactcag gtacaatgac atctggttca tacgttaaga actctactaa aggtaaacgt
    33421 gaacgtgtag gtcgtttatt acaaatgcac gctaactcac gtcaagaaat cgatactgta
    33481 tactctggag atatcgctgc tgcggtaggt cttaaagata caggtactgg tgatacttta
    33541 tgtggtgaga aaaatgacat tatcttggaa tcaatggaat tcccagagcc agttattcac
    33601 ttatcagtag agccaaaatc taaagctgac caagataaaa tgactcaagc tttagttaaa
    33661 ttacaagaag aagacccaac attccatgca cacactgacg aagaaactgg acaagttatc
    33721 atcggtggta tgggtgagct tcacttagac atcttagtag accgtatgaa gaaagaattc
    33781 aacgttgaat gtaacgtagg tgctccaatg gtttcatatc gtgaaacatt caaatcatct
    33841 gcacaagttc aaggtaaatt ctctcgtcaa tctggtggtc gtggtcaata cggtgatgtt
    33901 cacattgaat tcacaccaaa cgaaacaggc gcaggtttcg aattcgaaaa cgctatcgtt
    33961 ggtggtgtag ttcctcgtga atacattcca tcagtagaag ctggtcttaa agatgctatg
    34021 gaaaatggtg tcttagcagg ttatccatta attgatgtta aagctaaatt atatgatggt
    34081 tcataccatg atgtcgattc atctgaaatg gccttcaaaa ttgctgcatc attagcactt
    34141 aaagaagctg ctaaaaaatg tgatcctgta atcttagaac caatgatgaa agtaactatt
    34201 gaaatgcctg aagagtacat gggtgatatc atgggtgacg taacatctcg tcgtggacgt
    34261 gttgatggta tggaacctcg tggtaatgca caagttgtta atgcttatgt accactttca
    34321 gaaatgttcg gttatgcaac atcattacgt tcaaacactc aaggtcgcgg tacttacact
    34381 atgtacttcg atcactatgc tgaagttcca aaatcaatcg ctgaagatat tatcaagaaa
    34441 aataaaggtg aataatataa cttgttttga ctagctagcc taggttaaaa tacaaggtga
    34501 gcttaaatgt aagctatcat ctttatagtt tgattttttg gggtgaatgc attataaaag
    34561 aattgtaaaa ttctttttgc atcgctataa ataatttctc atgatggtga gaaactatca
    34621 tgagagataa atttaaatat tatttttaat tagaatagga gagattttat aatggcaaaa
    34681 gaaaaatttg atcgttctaa agaacatgcc aatatcggta ctatcggtca cgttgaccat
    34741 ggtaaaacaa cattaacagc agcaatcgct actgtattag cgaaaaatgg tgactcagtt
    34801 gcacaatcat acgacatgat tgacaacgct ccagaagaaa aagaacgtgg tatcacaatc
    34861 aatacttcac acattgagta ccaaactgac aaacgtcact acgctcacgt tgactgccca
    34921 ggacacgctg actatgttaa aaacatgatc actggtgctg ctcaaatgga cggtggtatc
    34981 ttagtagtat ctgctgctga cggtccaatg ccacaaactc gtgaacacat tcttttatca
    35041 cgtaacgttg gtgtaccagc attagtagta ttcttaaaca aagttgacat ggttgacgat
    35101 gaagaattat tagaattagt agaaatggaa gttcgtgact tattaagcga atatgacttc
    35161 ccaggtgacg atgtacctgt aatcgctggt tcagcattaa aagctttaga aggcgatgct
    35221 caatacgaag aaaaaatctt agaattaatg gaagctgtag atacttacat tccaactcca
    35281 gaacgtgatt ctgacaaacc attcatgatg ccagttgagg acgtattctc aatcactggt
    35341 cgtggtactg ttgctacagg ccgtgttgaa cgtggtcaaa tcaaagttgg tgaagaagtt
    35401 gaaatcatcg gtttacatga cacatctaaa acaactgtta caggtgttga aatgttccgt
    35461 aaattattag actacgctga agctggtgac aacattggtg cattattacg tggtgttgct
    35521 cgtgaagacg tacaacgtgg tcaagtatta gctgctcctg gttcaattac accacatact
    35581 gaattcaaag cagaagtata cgtattatca aaagacgaag gtggacgtca cactccattc
    35641 ttctcaaact atcgtccaca attctatttc cgtactactg acgtaactgg tgttgttcac
    35701 ttaccagaag gtactgaaat ggtaatgcct ggtgataacg ttgaaatgac agtagaatta
    35761 atcgctccaa tcgcgattga agacggtact cgtttctcaa tccgtgaagg tggacgtact
    35821 gtaggatcag gcgttgttac tgaaatcatt aaataatttc taatttctta gattttatat
    35881 aaaaagaaga tccctcaatc gaggggtctt ttttttaatg tgtaaatttt gtaatggctg
    35941 ttcaatttag aagaacaata attgatgaga ggttgactaa taaaacttat aactgataat
    36001 acagtttaaa taaaattgtt gagccttgga cattgtaaaa tgctcctctc aaagttttca
    36061 tttttcaatg tctactttga aggagcatta cattagttta tgtctcagat tcatatcttt
    36121 caattaattt aaatgcttaa tttgttttaa atacttgctc taattcaatg attttcaaaa
    36181 atacagctac agcgtatttt aatgattttt catcaatatc aaatttggga ttatggtgtg
    36241 gcgctgtaat acctttactt tcattaccgc aaccagtcag aaagaatgca cctggtcgta
    36301 ctttcaaata atgtgaaaaa tcttatccaa tcatcattaa atctgattca ttaaagcgta
    36361 catgtaagtc atttgttgct tctttaataa cttgatatgc tttctcgttg ttatggacag
    36421 gcaagtatcc tttaatataa ttcaagtcat attcaatgtc atttgcaatt gccaaacctt
    36481 gtagtagctt atccatttta tgcatgacat gttcttgtat tttcgagtca aatgtacgaa
    36541 ctgtaccttt acaaaatgct tgatcaggaa taacgctatc tgtggtgcct gcttgaatca
    36601 ttccaaatga aagtacagct tgtttaactg gatcaatcgt acgtgaaatt attttttgtg
    36661 cacttaaaat gaactctgcc atgattacta ttgggtcaat ggtttcatga ggtttggcac
    36721 catgaccgcc acgaccttta attgtgacac taaattcatc tggagaggcc atgattgccc
    36781 ccgcacgcga atgaatagtt ccagtaggat aaccactcca taaatgtgta ccgtaaattc
    36841 tatctacatt ttccagacat ccagcatcaa tcatttcttg agaaccacct ggcatgattt
    36901 cttcaccgta ctggaatatt aatacaacat taccttctaa taaatgttta tgttcatcta
    36961 aaatttctgc tacggtaagt aaaattgctg tatgaccatc atgcccacac gcatgcatac
    37021 atcctggatt tttagactta taaggcacat cgtttaattc ctcgacaggt aacgcatcaa
    37081 agtcagctct taatgcaatg gtaggtcctg tgcccaaacc tttaaatgtg gctttgatac
    37141 cattgcggcc gataggagtt tcaatatcac aagataactg gcttaattgg ttaacaatat
    37201 aatcatgtgt ttgaaattct tcaaaagata actcaggata ttggtgtaaa taacgtctga
    37261 gttgaattgt tttattttct ttattatttg ctagttgaaa ccaatctaac accctaatca
    37321 ctactttcta aaatattgtt tatagtataa cattttatga aattatcgta ctaaatgatt
    37381 gctttgagat attttatcta tgaatgataa ggctttcaag ttatgtagaa ttactgtatg
    37441 ataaaggtat taccaaacaa tacttaaggg ggattatata ctgtggttca atcattacat
    37501 gagtttttag aggaaaatat aaattatcta aaagaaaatg gtttgtataa tgaaatagat
    37561 acaattgaag gtgcaaacgg accagaaatc aaaatcaatg ggaaatcata cattaactta
    37621 tcttcaaata attatttagg actagcaaca aatgaagatt tgaaatcagc tgcaaaagca
    37681 gctattgata cacatggtgt aggtgcaggc gctgttcgta caatcaatgg tacattagat
    37741 ttacacgacg aattagaaga aacactagca aaatttaaag gaacagaagc tgcaatagct
    37801 tatcaatcag gatttaattg taatatggct gctatttcgg ctgttatgaa taaaaatgat
    37861 gctattttat cagatgagct taatcatgca tcaattattg acggatgtcg cttatcaaaa
    37921 gctaaaatta ttcgagttaa ccattcagac atggatgatt tacgtgcgaa agcaaaagaa
    37981 gcagttgaat caggtcaata caataaagtg atgtatatca ctgatggcgt ttttagtatg
    38041 gatggtgatg tggctaaatt acctgaaatt gtagaaattg cagaagaatt tggtttatta
    38101 acttatgttg acgacgctca tggttcaggt gttatgggca aaggcgctgg tacggttaaa
    38161 cattttggtt tacaagataa aatcgatttc caaattggta cgctttctaa agcaattggt
    38221 gtcgttggcg gttatgtagc aggtacaaaa gagttaatag attggttaaa agcacaatca
    38281 cgaccattct tattctctac atcattagca cctggggata ctaaagcaat cactgaagca
    38341 gttaaaaagt taatggcttc aactgaatta catgataaat tatgggacaa tgcacaatat
    38401 ttaaaaaacg gattgtcaaa attaggatat gatacaggag agtcagaaac tccgattaca
    38461 ccagtaatta ttggtgaaga aaaaacaact caagaattta gtaagcgttt aaaagacgaa
    38521 ggtgtctatg tgaaatctat cgttttccca acagtgccaa gaggtacagg acgtgtaaga
    38581 aatatgccta cagctgcaca tacaaaagac atgttagacg aagcaattgc ggcttatgaa
    38641 aaagtaggaa aagaaatgaa tttgatttaa tttaatatat atttattccc acggcaaata
    38701 tcgtcgtggg ttttttcttt tataaatgct tagtttatta acagtaagtt cttataacaa
    38761 tgtttagtgt tgtcaaccat taaagtttga attttaaaag catcttgtag aatttagttg
    38821 tatttttacg attaaattca ttttgattat ttttgataat aagcatttta atagtaatac
    38881 atgtttatag tgtgtagcat atgtctatac tagtagtaac tatatagaga aagtaggaat
    38941 aaactatgtc acaagatgta aataaattaa gtaagcaacc aacgccagat aaagcagaag
    39001 ataacgcatt tttcccatca ccatattccc ttagtcaata tacagcacct aaaacagatt
    39061 ttgatggtgt tgaacacaaa ggtgcctata aagatggtaa atggaaagta ttgatgattg
    39121 ctgctgaaga gagatatgta ttattggaaa atggaaaaat gttctctacg ggtaatcatc
    39181 ctgttgaaat gttattacct ttacatcatt taatggaagc aggttttgac gttgatgttg
    39241 cgacattatc tggttatcca gctaaattag aattatgggc tatgccaact gaagacgagg
    39301 cagttataag cacttataat aaattgaaag aaaaattaaa acagccaaaa aaattagcag
    39361 atgtgattaa aaatgaatta ggacctgatt cagactattt atctgtcttt atcccaggcg
    39421 ggcatgctgc agttgttggt atttctgaaa gtgaggacgt tcaacaaaca ttagattggg
    39481 cattagaaaa tgaccgcttt atagttacat tatgtcatgg accagccgca ctactttcag
    39541 caggtcttaa cagagaaaaa tctccattag aaggatactc tgtttgtgtt ttccctgact
    39601 cattagatga aggtgcaaat attgaaatag gttatttacc tggacgtttg aaatggttag
    39661 ttgctgattt attaactaaa caaagtttaa aagtagttaa cgacgatatg acaggaagaa
    39721 cgttaaaaga tcgtaaatta ttaacaggtg acagtccttt agcttcaaat gagttaggaa
    39781 aattagcagt taatgaaatg ttaaatgcaa tacaaaataa ataattaaat attaattaga
    39841 ggagccccat atgtaaatgt atgagggctc tttttttggc aaaattttag tgatacttgt
    39901 aaaatagaac ctattatgag tatgatttaa gaaaacgctt gaaaaactaa taactgcaac
    39961 tagcgatatg gaggaaacat gatgtcttat agcattggaa ttgattatgg aactgcttca
    40021 ggccgtgtgt ttttaattaa tacaactaac ggtcaagtag tatcaaaatt tgtgaaacca
    40081 tatacacatg gtgtcattga gggtgaatta aatggtttaa aaataccaca tacatatgca
    40141 cttcaaaata gtaatgatta tttagaaatt atggaagaag gaatatcata tatagtacgt
    40201 gaatcaaaaa tagatccagt caatatagta ggtattggta tagactttac ttcatctact
    40261 attattttta ctgacgaaaa ccttaacccg gtacataatt taaaacaatt taaaaacaat
    40321 ccacatgcgt atgtgaaact ttggaaacat catggtgcat ataaagaagc agagaaatta
    40381 tatcaaactg ctattgaaaa taataataag tggttaggcc attatggata taatgttagt
    40441 agtgaatgga tgattcccaa aataatggaa gtcatgaatc gagcaccaga aattatggaa
    40501 aaaacggctt atattatgga agcgggcgat tggattgtaa ataaattaac taataaaaat
    40561 gtacggtcga attgtggatt aggtttcaaa gcattttggg aagaagaaac agggtttcat
    40621 tatgatttgt ttgataaagt agaccccaaa ttatcaaaag taattcaaga taaagtatct
    40681 gcaccggttg ttaatattgg tgaagcagta gggaaactgg atgataaaat ggcacagaaa
    40741 ttaggattat caaaagacac tatggtaagt ccttttatta ttgatgccca tgctagttta
    40801 ttaggtattg gatctgaaaa agataaagaa atgactatgg tgatgggaac aagcacatgc
    40861 catcttatgt taaatgaaaa gcaacatcaa gtgccaggta tatcaggttc tgtaaaagga
    40921 gcaattattc cagaattatt tgcttatgaa gcggggcaat cagcagtagg tgatttgttt
    40981 gagtatgtcg ctaagcaagc accaaagtca tatgtagatg aagcagcaaa tagaaatatg
    41041 actgtatttg aattaatgaa tgaaaagata aaacatcaaa tgccaggtga aagtgggctc
    41101 attgctcttg attggcataa tggaaatcga agtgtattaa gtgatagcaa tttaacaggt
    41161 tgtatctttg gattaacttt acaaactaag catgaggata tttatagagc atatttagaa
    41221 gctacagcat ttggtactaa gatgattatg caacagtatc aagattggca tatggaagta
    41281 gaaaaggtat ttgcatgtgg cggtatacct aaaaagaatg ctgttatgat ggatatctat
    41341 acgaatgtac tgaataaaaa actaattgtt atggatagtg agtatgcacc agcaatagga
    41401 gcagcaatat taggtgcagt cagtggtggc gcacataatt caattaatga tgcagttgat
    41461 gctatgaaag agccaatttt atacgaaatt aatccagaag cggaaaaagt acaaaggtat
    41521 gaaacattat ttaaagctta taaggcttta catgatatcc atggttataa aaaagctaat
    41581 ataatgaaag atatccagag tttaagagtt gagggataaa aaatttaatt tgacagtaat
    41641 tagcaatatt aaacgctata aattactaaa atcaattaat acagctagaa tttccattta
    41701 cagaaatatc agcaaaatgt acatgtcata tatacaagaa agcgctttta tattataatt
    41761 attatgaaaa tgaatattat gtcctcttgt gatggactat ggttatatag aagagacaaa
    41821 ggagatattt ctatgaaaaa aattatgatt actggtgcat taggacaaat tggtacagaa
    41881 ttagttgtta agtgcagaga aatttatggg acagataatg ttcttgctac agatattagg
    41941 gaacctgaag cagactcacc tgtacaaaat ggaccatttg aaatcttaga cgtaacagat
    42001 cgtgaccgta tgtttgagtt agttagggac tttgaagcgg atagtctaat gcatatggca
    42061 gcattattat cagcaactgc tgagaaaaat ccaattcttg cttgggattt aaatatgggt
    42121 ggattaatga atgcattaga agctgcaaga acttataatt tgcacttttt cacaccaagt
    42181 tcaattggtg catttggaga ctcaactcct aaagttaata cgccacaagt aacgattcag
    42241 caacctacga caatgtatgg tgtaaataaa gtagctggcg aattgttgtg tcaatattat
    42301 ttcacacgat ttggtgtaga tacaagaagt gttagattcc ctggtttaat ctcgcatgtt
    42361 aaagaaccgg gtggtggcac aacggactac gcagttgaaa tttacttcaa ggctgtaaga
    42421 gaaggacatt atacaagctt catagataaa gggacatata tggatatgat gtatatggat
    42481 gatgcgattg atgcaattat taaacttatg gaagcagacg atgcgaaatt agaaacaagg
    42541 aatggttata atttaagtgc aatgagcttt gatccagaaa tggttaaaga agcaatacaa
    42601 gaatattacc cagatttcaa attagaatat gatgttgacc ctattagaca aggtatcgct
    42661 aatagttggc cggattctat tgatacaagc tgttcacgtg gcgaatgggg atttgatcct
    42721 aaatatgatt tagtgagcat gactaaatta atgttagaag ctattgaaca aaaagatact
    42781 gttaaaaata ataactaatc a
//
LOCUS       DAKSQE010000007        42781 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.007, whole genome shotgun sequence.
ACCESSION   DAKSQE010000007 DAKSQE010000000
VERSION     DAKSQE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 42781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42781
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.007"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            140..1129
                     /gene="ccpA"
                     /locus_tag="O0V33_000265"
     CDS             140..1129
                     /gene="ccpA"
                     /locus_tag="O0V33_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCX8974955.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGKLIEKGAKSFALVGGEHSKKAQEDVLAGLTEVLNKNSLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
     gene            complement(1227..2396)
                     /locus_tag="O0V33_000266"
     CDS             complement(1227..2396)
                     /locus_tag="O0V33_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HCX8974956.1"
                     /translation="MQQHLSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATDDELMLIHKYDYVEAIKHASHGIISEDEAMKYGLNDEENGQFKHM
                     HRHSATIVGGALTLADLIMSGKALNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDSSFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADTYTNGKIIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(2421..3053)
                     /locus_tag="O0V33_000267"
     CDS             complement(2421..3053)
                     /locus_tag="O0V33_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCX8974957.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTHEDLKALTFDAYLTAFRD
                     AEDQFEALLEITTLPEGRIYVARQDKLIVGYVTFHYPDEIERWSTGNLPYLIELGAIE
                     VSINFRQLQLAEKLIQLSLSTPEFEDYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSNPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            3222..4928
                     /gene="acsA"
                     /locus_tag="O0V33_000268"
     CDS             3222..4928
                     /gene="acsA"
                     /locus_tag="O0V33_000268"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCX8974958.1"
                     /translation="MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEHRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRLFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
     gene            5368..7035
                     /locus_tag="O0V33_000269"
     CDS             5368..7035
                     /locus_tag="O0V33_000269"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCX8974959.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVQGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKAREAGFDPAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWEKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            complement(7281..7370)
                     /locus_tag="O0V33_000270"
     CDS             complement(7281..7370)
                     /locus_tag="O0V33_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8974960.1"
                     /translation="MLLTTPIRWYLIDNNYQYQLLVLIVAHPN"
     gene            7369..10041
                     /gene="harA"
                     /locus_tag="O0V33_000271"
                     /pseudo
     CDS             7369..10041
                     /gene="harA"
                     /locus_tag="O0V33_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
     gene            complement(10247..11152)
                     /locus_tag="O0V33_000272"
     CDS             complement(10247..11152)
                     /locus_tag="O0V33_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCX8974961.1"
                     /translation="MTNQDNNHQLNHRIYQFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTNQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GVPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITKKEYNDALKYDIKSHLLNRKKR"
     gene            11533..12795
                     /locus_tag="O0V33_000273"
     CDS             11533..12795
                     /locus_tag="O0V33_000273"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCX8974962.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            complement(12889..14163)
                     /locus_tag="O0V33_000274"
     CDS             complement(12889..14163)
                     /locus_tag="O0V33_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCX8974963.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDSEDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
     gene            14335..14952
                     /locus_tag="O0V33_000275"
     CDS             14335..14952
                     /locus_tag="O0V33_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCX8974964.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLITGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            15080..16546
                     /locus_tag="O0V33_000276"
     CDS             15080..16546
                     /locus_tag="O0V33_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HCX8974965.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHAAPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGAVALAAALGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFVYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIVATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHNHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDQ
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            16652..17782
                     /locus_tag="O0V33_000277"
     CDS             16652..17782
                     /locus_tag="O0V33_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCX8974966.1"
                     /translation="MKKILFDVDGVFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYRDEPVELKLQNISTNIADRFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDANLFSLKGALWTLAQEVYQEWYLGSKLYEEVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGFLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALNGNKRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLSGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(17743..17838)
                     /locus_tag="O0V33_000278"
     CDS             complement(17743..17838)
                     /locus_tag="O0V33_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8974967.1"
                     /translation="MKPLVQSFTSSYAVKQQSNYKLSSTPLSSPK"
     gene            complement(17932..19536)
                     /locus_tag="O0V33_000279"
     CDS             complement(17932..19536)
                     /locus_tag="O0V33_000279"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCX8974968.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNINIDAATLKGILVINAPDGNTI
                     SATEHSLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAD
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     YLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            complement(19523..20683)
                     /locus_tag="O0V33_000280"
     CDS             complement(19523..20683)
                     /locus_tag="O0V33_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HCX8974969.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINYLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKERFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLK
                     GQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            complement(20798..21244)
                     /locus_tag="O0V33_000281"
     CDS             complement(20798..21244)
                     /locus_tag="O0V33_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCX8974970.1"
                     /translation="MHQHDFKVKTSWQGGRNNVGNVQGDILSENISIPASLGGVGIGT
                     NPDEMLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPHI
                     QIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            21324..22067
                     /locus_tag="O0V33_000282"
     CDS             21324..22067
                     /locus_tag="O0V33_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCX8974971.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRIADYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKFDKKLLIELKSPNLYPGIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRKYWYKKPNFSRIAQIASYVNPNYALVTRKFVDEAHHHQLQVMPY
                     TVNKLKTGEKLRQIGVDGLITDDPKLFIK"
     gene            complement(22283..22885)
                     /gene="rpsD"
                     /locus_tag="O0V33_000283"
     CDS             complement(22283..22885)
                     /gene="rpsD"
                     /locus_tag="O0V33_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCX8974972.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPSQTISVREKSQKLNVIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(23129..23593)
                     /locus_tag="O0V33_000284"
     CDS             complement(23129..23593)
                     /locus_tag="O0V33_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCX8974973.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLDQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQVVADVHQFKGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
     gene            23730..25424
                     /gene="ezrA"
                     /locus_tag="O0V33_000285"
     CDS             23730..25424
                     /gene="ezrA"
                     /locus_tag="O0V33_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCX8974974.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     SQCEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            complement(25498..25633)
                     /locus_tag="O0V33_000286"
                     /pseudo
     CDS             complement(25498..25633)
                     /locus_tag="O0V33_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792848.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25783..26931
                     /locus_tag="O0V33_000287"
     CDS             25783..26931
                     /locus_tag="O0V33_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCX8974975.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     DQLLQQAKVQINDMVNTNMTYDVVFTSGATESNNIALKGVAYRKFDTAREIITSVLEH
                     PSVLEVVRYLEEREGFKVKYVDVTKDGTIDLQHFNDLMSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAEVIKNYPKAHFHVDAVQAFGKISMDFNNVDSISLSGHKFNGLKGQGVLLVNH
                     IQNIEPIIQGGGQEYGVRSGTVNLPNDIAMVKAMKMANENLEALNAFVTELNNDVRQF
                     LSQYQGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            26931..28154
                     /gene="thiI"
                     /locus_tag="O0V33_000288"
     CDS             26931..28154
                     /gene="thiI"
                     /locus_tag="O0V33_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCX8974976.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITNRLSKIFGIKSISPVLKVEKSIEAMSAATIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGTVLKQIENVSVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGAYAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            28198..28968
                     /locus_tag="O0V33_000289"
     CDS             28198..28968
                     /locus_tag="O0V33_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HCX8974977.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSAIKFIRSGKVDLYVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAILFAALFILIGFYDGFVGGGTGS
                     FMLFVLLIFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLVMAASMIVGSY
                     AGAHFAIKQGVGYVKVLFIIITAILILKNAFDYIQQFV"
     gene            29066..29560
                     /gene="tpx"
                     /locus_tag="O0V33_000290"
     CDS             29066..29560
                     /gene="tpx"
                     /locus_tag="O0V33_000290"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCX8974978.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSEASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDADNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            29683..30630
                     /locus_tag="O0V33_000291"
     CDS             29683..30630
                     /locus_tag="O0V33_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCX8974979.1"
                     /translation="MAEQQTIMERLFHSLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGTGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSRDFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKSVEVLLANIPDFKNPSQFQGFMTELNQWMDTNRPKK"
     gene            30717..31919
                     /locus_tag="O0V33_000292"
     CDS             30717..31919
                     /locus_tag="O0V33_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCX8974980.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLVERIGLKDSIFT
                     IEVDGEKVKTVQDIKDHVEAVDIMLDAFKKHNIINDINDIDGTGHRVVHGGEKFPESA
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLQRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTYGGLK"
     gene            complement(32166..32666)
                     /locus_tag="O0V33_000293"
     CDS             complement(32166..32666)
                     /locus_tag="O0V33_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCX8974981.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            32807..33925
                     /gene="ald"
                     /locus_tag="O0V33_000294"
     CDS             32807..33925
                     /gene="ald"
                     /locus_tag="O0V33_000294"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCX8974982.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALHL"
     gene            34053..34157
                     /locus_tag="O0V33_000295"
     CDS             34053..34157
                     /locus_tag="O0V33_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8974983.1"
                     /translation="MILIHYMLLMNIPAYVFLACLLHIAFRLGQKFMS"
     gene            complement(34235..35290)
                     /locus_tag="O0V33_000296"
     CDS             complement(34235..35290)
                     /locus_tag="O0V33_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCX8974984.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEINKIRKAAELADKCIEIGVSYLKES
                     VTEREVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSQEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            35465..36154
                     /locus_tag="O0V33_000297"
     CDS             35465..36154
                     /locus_tag="O0V33_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCX8974985.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTEATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(36461..36874)
                     /locus_tag="O0V33_000298"
     CDS             complement(36461..36874)
                     /locus_tag="O0V33_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCX8974986.1"
                     /translation="MYKNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQ
                     ASIKAGVHLNKLTEERSKRLKKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEI
                     VVLSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
     gene            37079..38377
                     /locus_tag="O0V33_000299"
     CDS             37079..38377
                     /locus_tag="O0V33_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCX8974987.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVIDFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANQ
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
     gene            38688..39629
                     /gene="pde2"
                     /locus_tag="O0V33_000300"
     CDS             38688..39629
                     /gene="pde2"
                     /locus_tag="O0V33_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HCX8974988.1"
                     /translation="MISTMNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGRKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFC
                     QVKITEDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGSEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVNSWDEFEQLAKALRTKLN"
     gene            39650..>42781
                     /locus_tag="O0V33_000301"
     CDS             39650..>42781
                     /locus_tag="O0V33_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCX8974989.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDTCIANNIKPIFGMTIYVTNGLNNIETVVLAKDNYGLKDLYQLSSEIKMNAL
                     EHVSFELLKRFSNNMIIIFKNVADEHRDIVRVFDSHEDTYLDHRSVLVQGIKHVWIQD
                     VCYQTRHDADTISALAAIRDNTKLDLIHDQEDFGAHFLTENEIHQLDVNPEYFTQADR
                     IAQKCNAELKYHQSLLPQYQTPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRREKVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILTQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGTKQNGYHEDISKQIFDFILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVEERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNNKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDLR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFY
                     DETIKQMTTLGYIN"
BASE COUNT        14786 a         6225 c         7849 g        13921 t
ORIGIN      
        1 agcgtttgtt tttatttcag gcttttcgct aaatttttcc aaacaaaaat atgttactgt
       61 aaattaaaat atggtaaact atgaaaatga aatgaaaaca tgttattata atgaataaaa
      121 cgtttacaag gaggaaatta tgacagttac tatatatgat gtagcaagag aagcgcgtgt
      181 ctctatggcc acagtgtcgc gtgttgttaa tgggaaccaa aatgttaaag cagaaactaa
      241 aaataaagtt aacgaagtca ttaagcgttt gaattatcgt ccaaatgctg ttgctagagg
      301 tttagctagt aaaaagacaa caacagtagg tgtgatcatt ccagatatat ctaatatcta
      361 ttattcacaa cttgctcgtg gacttgaaga tattgcaaca atgtataaat atcactcaat
      421 tatttcaaat tcagataacg atcctgaaaa agaaaaagaa atttttaata acttattaag
      481 taaacaagtt gatggtatta ttttccttgg tggtacaatt actgaagaaa tgaaagaatt
      541 gataaatcaa tcatctgtac ctgtagtagt atcaggaaca aatggtaagg atgcacatat
      601 agcatctgtt aatattgatt ttactgaagc tgcgaaagaa attacgggaa aattaattga
      661 aaaaggcgct aaatcatttg ctttagtagg tggagaacat tctaaaaaag ctcaagaaga
      721 tgtattagca ggtttaactg aagtgttaaa taaaaatagt cttcaattag gtgatacatt
      781 gaattgttct ggtgctgaaa gttataaaga aggcgtaaaa gcttttgcta aaatgaaagg
      841 taaccttcca gatgctatat tatgtatcag tgacgaagaa gctattggta tcatgcatag
      901 tgcaatggat gctggtatta aagttccaga ggaattacaa attattagtt tcaataatac
      961 acgcttagtt gagatggtta gaccacaact ttctagtgtt attcagccat tatatgatat
     1021 cggtgcagta gggatgcgct tattaacaaa atatatgaac gatgaaaaga tagaagaacc
     1081 caatgtagtt ttaccacaca gaattgaata ccgaggaact acaaaataaa ttcacaaaat
     1141 taggcattca tctaacgact cgacttatac gggtgttgga agaatgcctt ttattcatca
     1201 tttaaaatcg ttgcagatta ggtgacttat tgacgagtag attcgtacca actcgctata
     1261 tgcaaagcta attttttatt tttctcacta atttcttttg tgcgggggac ataggtataa
     1321 tcatttaaac gatcttccca tcttttaggt aataattcag atgaataatg tttccattta
     1381 ttaatccatt ctaacggtaa ataaccactt tgaattggtt gatcaattaa acttaagaat
     1441 acatgactcc atgcacgtgg tacaactctc caaatattgt agcctccgcc accaaacata
     1501 attatctttc cattcgtata agtatcagct aaatatttta caaaatatgg aatttcatat
     1561 aatgaatgta acgtacaatt tagatgagtt agtggatcac gataatgtat atcgacacca
     1621 tttacgctta gaataatatc aggtttaaaa ctctttacga ctggctcaac tgttaattta
     1681 aaacactcca aaaatgatga atcttcggta tacggttcaa gtgggacatt tacagtgtgt
     1741 ccatagccga tatcttcacc gcgctcagta tagtgaccag agcctgggaa aagaaacttt
     1801 ccggtttcat ggatagaata agtagtaaca tggttatcag catagaaact ccattgtgta
     1861 ccatccccat gatgtgcatc ggtatctatg attaaaacgc gttgattgta ttctttagct
     1921 aagtattgtg cagtaattgc aatatcattg tatatacaaa aaccacttgc tcgaccaggt
     1981 tgagcgtgat gcaaaccacc acctaagtga caaccattta atgctttgcc tgacataata
     2041 agatctgcta aagttaaagc gcctccaaca attgtggcac tatggcggtg catatgctta
     2101 aattgaccat tctcttcatc atttaatcca tatttcatag cctcatcttc actgataatg
     2161 ccatgtgaag catgcttaat agcttcgacg taatcatatt tatgaattaa cattaattcg
     2221 tcatctgttg caattctagg ttgtactatt tgttctggag acaataaatt tgcattcaaa
     2281 agtagctctg ttgttaattt taaacgcatt tgattgaagg gatgttggtc atgaaatcga
     2341 tattgtaata acttatctga atatacatat gcagtttttg ataaatgttg ttgcatataa
     2401 tccctccgat attccaaaaa ttaaaagaaa aaccgattca tataacgaat atcatcaaac
     2461 gcttgttgct gttctaatgt aatatttttg ccaattcttg ccattaaaca attagctgga
     2521 ttacttgtta tttctggatc atctgtagcg aatatttcaa gtccaccagt tgccattaac
     2581 cgctgcatta attttttata gtcaaataca tctaactttg aattttttaa atcccaatgc
     2641 cagtaatatt ctgtagttat aacgatataa tcctcgaatt ctggtgtaga aaggctaagt
     2701 tgtattaatt tttctgcaag ttgtaattgc ctaaaattga tgctgacttc aattgccccc
     2761 aattcgatta aatatggaag gttacctgta gaccagcgct caatttcatc aggatagtgg
     2821 aaagtgacat aacccacaat gagtttatct tggcgagcaa cataaattct accttctggt
     2881 aatgttgtaa tttctaacaa agcttcaaac tgatcttcag catctctaaa tgcggttaaa
     2941 tacgcatcga aagtaagcgc tttcaaatct tcgtgtgtta aaggaccttc aataacaaat
     3001 tgcttgtcat gtatgtaata atcttcggat tgatacgtct ttaaatgatt catattttca
     3061 actcctcaat cgacgtcgtg attgtattaa attcattata tagaaaattt acaataatta
     3121 atactagaaa aaggataaaa gtaaaaattt tgaataatta gaaatgttat gtataatatt
     3181 gagaaagaaa gcgttttcac ataacaaagg gggagtttca aatgaaagtc gaagtttata
     3241 aaggagcgca aggcaaacat aaccttaaag attatgaaga aacatataat acttttgatt
     3301 ggaaagacgt agaacaagca ttttcttgga gtgaaactgg aaaaatgaac atggcatatg
     3361 aatgtataga tcgccatgta gatcaaggat taggggataa aatagcatta aattacaaag
     3421 atgaacacag aaaagaatcg tatacttata aagatatgca acggttatct aataaagcgg
     3481 cgaatgtttt gtctgaacat gcagaagttg acaaaggtga cagagtattt atatttatgt
     3541 cgcgtacacc tgaactatat tttgcgttgt taggtgtttt aaaaattggt gcaattgttg
     3601 ggccgttatt tgaagcattt atggaaaagg cggttgcgga tagattagag aacagtgaag
     3661 ctaaagtgtt aattactaat aaggcattgt tacctcgagt acctgtagat aaattaccga
     3721 acttgaaaaa aattgttgtc gtagatgagg atgtagaaga caattacata gacttcatta
     3781 gtttgatgga aactgctagc gatgaatttg acattgaatg gttaaagtcg gatgatggtt
     3841 tgattttaca ttatacatca ggttctactg ggcaacctaa aggtgtattg catgttcaac
     3901 aagcgatgtt agtgcactat atttctggaa aatatgtatt agatttacaa gaagatgatg
     3961 tttattggtg tacagcagat ccaggttggg ttacaggaac atcttatggt atttttgcac
     4021 catggttaaa tggcgctaca aattgtatag ctggtggtcg cttttcacca gaacaatggt
     4081 atagtatgat tgaagatttt aaagtgacga tttggtatac ggcaccaaca gctttaagaa
     4141 tgttaatgag tgctggtgac gatattgttg agaaatatga cttgtcatcg ttacgttcga
     4201 ttctatcagt aggtgagcct ttaaatcctg aagttataaa atgggcgaaa aaagtatacg
     4261 gtttaacggt gttagatact tggtggatga cagaaacagg tggacatatg attgttaact
     4321 atccaacgat ggacgtcaag cttggctcaa tgggcaaacc attacctggt attcaagctg
     4381 caattatcga tgatgcaggt aatgaattac caccaaatcg aatgggcaac cttgctataa
     4441 aaaaaggctg gccatcaatg atgtatcgta tctggaagaa tccagaaaaa tataagtcat
     4501 attttattgg agactggtat gtatctggtg attcggcata taaagatgaa gatggttact
     4561 tttggttcca aggacgtgtt gatgatgtaa ttatgacagc tggcgaacga gttgggccat
     4621 ttgaggttga gtctaaattg gttgaacacg aagcagttgc cgaagcagga attattggta
     4681 aacctgatcc ggttcgcggt gaaataatta aggcgtttgt tgcactgaga aaaggatatg
     4741 aaccaacaga cgaattaaaa gaagaaattc gtttatttgt taaagaaggt ttgtcggcgc
     4801 atgcagcacc acgtgaaatc gaatttaaag ataaattacc taaaacacgc tcaggtaaaa
     4861 ttatgagacg tgtattaaaa gcttgggaat taaatttaga tgcaggggat ttaagtacaa
     4921 tggaataatg acatgaatgt tatagaagat ttctttcgaa gaataaaggg tgacaacata
     4981 tttcatgtta atgtttaaat aatcgtttac tttacgataa gcaatataaa gagctgttaa
     5041 cttgtgtcat atcatttcgt agaaaacatt tgaaaatgat gacataacaa taatggcata
     5101 tctttatatt gctttttatt tttaatatga cctttggaag atgactattt taaataatag
     5161 aaaaatatag ttatcaatag tatcaagcgc taaaagttgt ataatacaaa actttaataa
     5221 gtgaatttat tgcaaaaatg aaagcgctaa cccgatttag tcgacaagtt tttaacagtt
     5281 cgttattata tgaatgtaag taaaaatttc ttagctacaa cttacatatt ataaatgcat
     5341 aaattaaaca aaaaggggcg aaaaaagttg actcatttat cagatttaga tattgcgaat
     5401 caatcaacac tacaaccaat taaggatatt gctgcatcag taggtatttc agaggatgca
     5461 ttagaacctt atggtcatta caaagctaaa atcgacatta ataaaattac gccaagagaa
     5521 aacaaaggga aagttgtttt agtaactgcg atgagcccaa caccagctgg tgaaggtaaa
     5581 tcaacggtta cagttggttt agctgatgca ttccatgagt taaataaaaa cgttatggtt
     5641 gcattaagag agcctgcttt aggaccaaca tttggtatta aaggtggtgc aactggtggt
     5701 ggttatgcgc aagtcttacc tatggaagat atcaacttac atttcaacgg agatttccat
     5761 gcgattacaa ctgctaataa tgcattgtct gcgtttatcg ataatcatat tcaccaaggt
     5821 aatgaattag gaatcgatca aagacgtatt gaatggaaac gtgtattaga tatgaatgat
     5881 cgtgcactta gacatgtaaa cgttgggtta ggtggaccta caaatggtgt accacgtgaa
     5941 gatggcttta atattacagt agcgtctgaa attatggcga ttttatgctt aagtagaagt
     6001 attaaagact taaaagataa aattagtcgt attactattg gttacactag agatcgcaag
     6061 ccagttacag ttgcagattt aaaagtgcaa ggtgcacttg caatgatttt aaaagatgca
     6121 attaaaccaa acttagtaca atcaattgaa gggacaccag cattagttca tggtggacca
     6181 tttgcgaata tcgcacacgg ttgtaactca attttagcaa ctgaaacagc acgtgattta
     6241 gctgatatcg ttgtaacgga agctggattt ggttcagact taggcgctga aaaattcatg
     6301 gacattaagg cgcgtgaagc tggattcgac ccagcagcag ttgtagttgt agcaacgatt
     6361 cgtgcattaa aaatgcatgg tggtgtagca aaagataatt taaaagaaga aaatgtagaa
     6421 gcagtaaaag caggaattgt taatttagag cgtcatgtta ataatattaa aaaattcggt
     6481 gtagaaccgg ttgttgcaat taatgcattt atacatgata ccgatgcaga agtagaatat
     6541 gtaaaatctt gggctaaaga aaataacgta cgaattgcct taactgaagt ttgggaaaaa
     6601 ggtggtaaag gtggcgttga cttagcaaat gaagtattag aagtcattga tcaacctaat
     6661 tcatttaaac ctttatatga attagaatta ccattagagc aaaagattga aaagattgtg
     6721 actgaaatct atggcggttc aaaagtaacg tttagtagta aagcgcaaaa acaattaaaa
     6781 caatttaaag aaaatggttg ggataattac ccagtatgta tggcgaaaac acaatattca
     6841 ttctcagatg atcaaacgtt gttaggtgca ccatcaggat ttgaaattac aattcgtgaa
     6901 ttagaagcga aaacaggtgc aggatttatc gtagcgttga caggtgcaat catgactatg
     6961 cctggtttac ctaaaaaacc agcagcatta aacatggatg ttactgatga tggtcatgca
     7021 attgggttat tctaatacat catgtcaatt gtttaataaa gataagtaaa tagtttaata
     7081 gaccggactg ttggagatgc attatttcag cagttcggtt tttttgctgt gctaaaaata
     7141 gattcaattt ggcgaatcta acggcaatgt ttgaagatgg ttaattaatg tattccaaga
     7201 taaaaagtgg gcttgaagaa taggaaagcg atgcaatgaa cattcatatt aaaaattttt
     7261 aataaaatag gttgcaatat ttaattggga tgcgctacaa ttaacactaa taattgatat
     7321 tgataattat tatcaattaa ataccatctt ataggagttg ttaacaacat gaacaaacat
     7381 cacccaaaat taaggtcttt ctattctatt agaaaatcaa ctctaggcgt tgcatcggtc
     7441 attgtcagta cactattttt aattacttct caacatcaag cgcaagcagc agaaaataca
     7501 aatacttcag ataaaatctc ggaaaatcaa aataataatg caactacaac tcagccacca
     7561 aaggatacaa atcaaacaca acctgctacg caaccaacaa acactgcgaa aacctatcct
     7621 gcagcggatg aatcacttaa agatgcaatt aaaaatcctg cagtagaaaa taaagaacat
     7681 gatataggtc caagagaaca agttaatttt caattattag ataaaaacaa tgaaacgcag
     7741 tactatcact ttttcagcat caaagatcca gcagatgtat attacactaa aaagaaagct
     7801 gaggttgaat tagacatcaa tactgcttca acatggaaga agtttgaagt ttatgaaaac
     7861 aatcaaaaat tgccagtgag acttgtatcg tatagtcctg ttccagaaga ccatgcctat
     7921 attcgattcc ctgtttcaga tggtacacaa gagctgaaaa ttgtctcttc aactcaaatt
     7981 gatgatggag ctgaaacaaa ttatgattat actaaattag tatttgctaa acctatttat
     8041 aacgatcctt cacttgtaaa atcagataca aatgatgcag tagcaacgaa tgatcaatca
     8101 agttcagacg caagtaatca aacaaacacg aatacatcta atcaaaatac atcaacgacc
     8161 aacaatacta atgatcaacc gcaggcaacg aacaatatga gttaacctgc acaaccaaaa
     8221 gcgtcagcga attcagatca agcatcaagc caaccagctc atgaaacaaa ttctaatgac
     8281 aatactaacg atgcaacgaa tgcatcaagt aatcagtcaa atgggtacca acagtatcca
     8341 ccagcagatg aatcactaca agatgccatt aaaaacccgg ctatcatcga taaagaacat
     8401 acagctgatc attggcgacc aattgatttt caaatgaaaa atgataaagg tgagagacag
     8461 ttttatcatt atgctagtac tgttgaacca gcaactgtta tttttacaaa aacaggacca
     8521 ataattgaat taggtttaaa gacagcttca acatggaaga aatttgaagt ttatgaaggt
     8581 gacaaaaagt taccagtcga attagtatca tatgattctg ataaagatta tgcctatatt
     8641 cgttttccag tttctaatgg ggcgagagaa gttaaaattg tgtcatctat tgaatatggt
     8701 gagaacttcc atgaagatta tgattataca ttaatggtct ttgcacagcc tattactaat
     8761 aacccagacg actatgtgga tgaggaaaca tacaatttac aaaaattatt agctccgtat
     8821 cacaaagcta aaacattaga aagacaagtt tatgaattag aaaaattaca agataaattg
     8881 ccggaaaaat ataaagcaga atataaaaag aaattagatc aaactagagt agagttagcc
     8941 gatcaagtta agtctgcagt gacagaattt gaaaatgtta cacctacaaa tgatcaatta
     9001 acagatgtac aagaggctca ttttgttgtt tttgaaagtg aagaaaatag tgagtcagtc
     9061 atggatggat ttgttgaaca tccattctat acagcaactt taaatggtca aaaatatgta
     9121 gtgatgaaaa caaaggatga cagttactgg aaagatttaa ttgtagaagg taaacgtgtc
     9181 actactgttt ctaaagatcc taaaaataat tctagaacgc tgattttccc atatgtacct
     9241 gacaaagcaa tttataatgc gattgttaaa gtcgttgtgg caaacattgg ttatgaaggt
     9301 caatatcatg tcagaattgt aaatcaggat atcaagacaa aagatgatga cacatcacaa
     9361 aataacacga ttgaacctct aaatgtacaa acaggacaag aagataaggt tactgcaacg
     9421 gatacagctg aaaatagcag cgctgcaaca aatccaaaag atgcgtctga taaagcagat
     9481 gtgatagaac cagagtctga cgtggttaaa gatgctgata gtagtgttga caaagatgtg
     9541 caaaatgatg ttgatcattt atccgatatg tcggataaca ctcacctcga taagtatgat
     9601 ttcaaagaaa tggatactca aattgctaaa gacactgata agggtgttga taatagcctt
     9661 ggaatgacat ctaatgtcga tactgacaaa gactctaata aaaatgaagg caaagttata
     9721 caactagcac ataataccga taaaaatcat actggaaaag cagcaaagct tgatggcgtg
     9781 agacaaaatt ataataatac agacaaagtt actgataaaa aaacaactga acatctgccg
     9841 agtgatattc ataaaactgt agacaaaaca gtgaaaacaa aagaaaaggc cggcacacca
     9901 tcgaaagaaa acaaacttag tcaatctaaa atgctaccaa aaactggaga aacaacttca
     9961 agccaatcat ggtggggttt atatgcgtta ttaggtatgt tagctttatt cattcctaaa
    10021 ttcagaaaag aatctaagta attaactgga tatagcatat gtatgattag ctttgtagac
    10081 aatgtgaaag caattaaatt ataaactatt gatttgttta atagctttcc tttagagtat
    10141 ttaaaataaa gaacagcagt gagaaatttt ctaattgaaa ataatcttac tgctgttttt
    10201 aatatttgga tggattattg tggttactta aaagagtgag catcaattaa cgcttttttc
    10261 gatttaacaa atgtgattta atatcatatt ttaatgcgtc gttgtattct tttttagtga
    10321 tttgatcttc gattaacata cgctttaata cataatgttg tctttgaata ctatatttca
    10381 aatctttatc cgattttaat gttccatctt tttcgtaggg tgtatagcca tatgggcttt
    10441 gcaacaaacc gataaggtat gcagattgtg caattgataa atcttttggt ggaacaccaa
    10501 ataaactata tgaagcggat gcaattccag aaatattagc gccattataa tctctaccga
    10561 agggaactat atttaaatac gtatatataa tttcatcttt tgagagtagg tgttctaatc
    10621 taattgctag gcgaagttca tttgcttttc tactatatgt tttttcgttg gtaagaactt
    10681 gatttttaac aagttgttgt gtaattgtgc taccacctga actttgatta gtattaaaaa
    10741 tatcttgtat cattgctctt aaaatcgcct ttggtaagat gccatcatgt ttataaaata
    10801 aagtgtcttc agatgacgtt aatgctttaa tgacatttgg acttgatgtt ttaggtccta
    10861 taatgagtga gttttgagaa tggttatact catataacaa atttttgtta ttatgatcta
    10921 ataattcatc gccaggtatt tgtcgaactt tttttattaa agcatcatct gataatgagt
    10981 cggacgtttt agttaaatga tgaaaatata aagacatcgc aatcacagcg atagcaacga
    11041 tggcaatgaa aatcataaat ataaaaacaa tgacatgttt gatagcttta tatatctttt
    11101 caaattgata tatacgatga ttcaattgat gattgttgtc ttgattcgtc atccttaacc
    11161 tcctaaatta cgtatagtat accatattta agcaatttga cttttatttt aacgttaatt
    11221 ataattaggg aaaatgcata aagaatagga taaaaggaca agtaatatta tttgaaatca
    11281 tcagagagct agtggtcgct gtgaactagt gtttttaata atatgaatac acctttataa
    11341 ctaacatgtc ctacgtttat taatgaacgt atcattaata gagtgaacat tatcttatga
    11401 ttatgtttaa gtttggtggt aacgtgcaat cgcgccctta catatttgtt gtaagggttt
    11461 ttttacgttc aaaaagcttt aaagtatttt aacaagtatt cactaaataa ttataaatag
    11521 gaggaaataa taatgacgaa tgtattaatt gaagatttaa aatggagagg tcttatttat
    11581 caacaaactg atgaacaagg tattgaagat ttattaaata aagaacaagt gacgttatac
    11641 tgcggtgctg atccaacggc agatagctta cacattggtc acttactacc atttttaaca
    11701 ttaagaagat ttcaagaaca tggacatcgt cctatcgttt taattggcgg tggtacaggt
    11761 atgattggtg atccatcagg taaatcagaa gaacgtgtgc tacaaacaga agaacaagta
    11821 gataaaaata tcgaaggtat tagtaagcaa atgcataata tttttgaatt tggaacagac
    11881 catggtgcag tgcttgttaa taatagagac tggttaggac aaatttcatt aattagcttt
    11941 ttacgtgact atggtaaaca cgtcggcgtt aattacatgt taggtaaaga ttcaatccaa
    12001 agtcgtttag aacatggtat ttcatataca gaattcacat acacgatttt acaagctatt
    12061 gatttcggtc atttgaatag agaattgaat tgtaagattc aagtaggtgg atcagatcaa
    12121 tggggtaata tcacaagtgg tattgaatta atgcgtcgta tgtatggtca aacagacgca
    12181 tacggtttaa ctattccgct tgtaactaaa tcagatggta agaaatttgg taaatctgag
    12241 tcaggtgctg tttggttaga tgctgaaaag acgagtcctt atgaatttta tcaattctgg
    12301 attaatcaat cagacgaaga tgtaattaaa ttcttaaaat actttacttt cttaggaaaa
    12361 gaagaaattg atcgcttaga acaatctaaa aatgaagcac cacatttacg tgaagctcaa
    12421 aaaacattag ctgaagaagt aactaaattt attcatggtg aagatgcatt aaatgatgca
    12481 atccgtattt cacaagcatt atttagtggt gatttaaaat cattatcagc gaaagaatta
    12541 aaagatggat ttaaagatgt gcctcaagtg acattatcaa atgacacaac aaatatcgtt
    12601 gaagtcctta ttgaaacagg catttctcct tctaaacgac aagcgcgtga agatgttaac
    12661 aatggtgcga tttatattaa tggtgagaga caacaagatg ttaattatgc tttagcacca
    12721 gaagataaaa ttgatggcga atttacgatt attcgtcgcg gtaagaaaaa atacttcatg
    12781 gttaactatc aataaatata attgcatagc taaataaatt agagcctact cattacattc
    12841 ttaggaatga atatgagtag gctcttaatg tacttttctg tctataaatt atctaaagaa
    12901 atctctatcg ccttggccag gtgattgacg ttctgatcgg ctttgacgtt ttggttgttc
    12961 tttttgttgt tttaatttca ctttaacttc tttagtttta ccatcacgga taacggtaac
    13021 agtgactgat tcaccaggct ttttattttc atataaatag cttcttaaat caacatcatc
    13081 tttaattttc ttgccatcaa tttctgtaat aatatcacct tttttaagat caatatcact
    13141 atcagctttt gcgacataaa taccgtcttc gctatcagta tgaagttgct cgcgctcttc
    13201 ttcaggtata tctttcaaat taattaaacc aataccaatc gaagggcggt ctattttacc
    13261 atgttttaca agttgttcaa ttgttacttt aacttcatta cttggaatag caaacccaat
    13321 accttcaact tgtgtcgcag caattttcat tgagttaata ccaactaaat taccattaat
    13381 atctactaat gcgccacctg agttacctgg gttaatagca gcatctgttt gaagaacgct
    13441 gacttttgta ttaccaccag ttgtctctgc gtcaatagta cgttcacttg ctgaaatgat
    13501 accagatgtt acagagttag caaattgtaa tcctaatggg ttacccattg cgaatacgct
    13561 atcgccagtt tgtacttttg aagagttggc gaattgaatc gctttaatac cttttgtatt
    13621 ttcaatttta agtacagcaa tatcagttac tgcatcttta ccaactaatt tcgctttaac
    13681 ttgtttttta ttatgtaatt ggactctaat ttcatttgcg ccatcaataa catgattgtt
    13741 tgtaacgata taagctgaat tgttgtttat ttgatagatg acacctgagc ctacaccggc
    13801 ttcagatggt ttagatgatt tgccttttaa taagtcgtct acacttgatg ctttttgcat
    13861 attaataact ccaacaattg taggggagac agattttatc atttcatgaa cggtaccgaa
    13921 tttcttgctt tgaccgtcta attgattgcc acctttatta tttgttgtct ggacagttga
    13981 accatcttta tttaaaattg tactatttaa tactttgcct ataccaagta ctagaagtgc
    14041 accaatgatt ccagcaatca atgcaacgat gactgtttta aaccatggaa atttaggtct
    14101 tctgtatcta ggtgtttggc tatggtttgt tgttgaatga tctgtatgat taaaatctga
    14161 catacttaac ctccattata tgatttatat atgctttaat tatgtctttt ttttataatt
    14221 attgctagta atacactata caactttaga tgtattttca gactatcttg ttctagtaaa
    14281 aatatagtga taaaatctag ttgttataaa caaataagtt aagtaggtgg cacaatgtat
    14341 tcagtgatta gtaagatttt gaatttcatt ttagttaaaa tgtcaaaatc attatacgta
    14401 atcggtaaag ataatattcc taaggatagt aaatatgtcg taacttgtac gcatgaaagt
    14461 tataacgaag tcattatgct aggtatggca ttatatccta atcaaattca ttatatggct
    14521 aaaaaagaat tattcaaaaa taaatggatt ggtaaatttt taacgtcttt aaatgcattt
    14581 cctgtagatc gtgaaaatcc aggacctagt actttaaaac gcccaatcaa tttgttgaaa
    14641 gataataaga cagttggtat cttcccaaca ggttctcgca catcgcaaga aggtgcacct
    14701 ttgaaacgtg gtgcttcaac aattgcaatg ttaagtaagt caccaatatt acctgttgct
    14761 tatgttggtc ctactaagat tcatggtctt ataactggac aagcatacat aaatattggt
    14821 aaaccaatcg atattaacga tttacctaaa gatttaaaac gaaacgagcg tatcgattac
    14881 attacaaaag agattgaaac acgaacagca aagttacaac aagaattgca tgaaattgta
    14941 aaaagtttat aaaataactt gaggtagaaa tatagctggt tagtaagcct aggtgactat
    15001 aacggagtat cgaagtagtt attctagtta cattagtaat ctttatattt ctaccttatt
    15061 ttaagaagga tgacattcaa tgtataaatt tttccaaaat ttagggcgtt cgttaatgtt
    15121 accagttgcc attttaccgg ctgcagcaat tattgccgga attggtaata cgttaaatgc
    15181 acttcatgcg gcgccgaaaa tcgcaatgtt ctttactacg gtaggtacta cgattttaga
    15241 gcaattaggt attttatttg caattggtgt agctatagga atggcaaaga aaaatgatgg
    15301 tgcagttgcg cttgcagctg cgttaggata ttttttagtt acggttgtat tatcaccaat
    15361 gaaattagcg cctttattag gaatgaaagc ttctgaaata aattcagctt ttgaaaagat
    15421 gaataatggc aatgtctttg ttggtattgt aataggttta atagcagctt atgcatacaa
    15481 taagtttagt gaaacagaat taccattagc attatcattt tttagtggta aacgtttagt
    15541 tccaattatg actgcatttt actgtacatt tttagttgtc atattgttat tcttatggcc
    15601 actactttat tcatggattg taaaatttgg tgaatctata gttggtttag gatcatttgg
    15661 ggctttcgta tatggtgttg ccaacagatt attaattcct acaggattac atcatgcact
    15721 gaatagtgtg ttctggtttg atacgattgg tattaatgat attggtaaat tccaaagtgg
    15781 caaagatgct ataaaaggta ttacaggacg ctatcaagca ggattcttcc caatcatgat
    15841 gttcggtatt ccagcggctg cgttagcaat gtatcataca gctaaaacta ctcagaaaaa
    15901 acaagtttac ggttggttcc tagcgagttc agttgcagca tttttcgttg gtgttacaga
    15961 gccgattgag tttgcattta tgtttgttgc gccaatctta tatgttgttc atgcgttatt
    16021 aacaggatta tctttattta tcgtagctac attccattgg acagctggat tttcatttag
    16081 tgcaggtttg atagattatg tgttatcact aattaatccg gtatcaaatc atccattgat
    16141 gttgcttgtt caaggtgttg tatttttcat cttatactat gtgattttcc gtgtagtaat
    16201 ccaagtattt aacttgaata cgattggtag aggtgaaaat gaattagttg atccaacagt
    16261 tgtaaaagat aatattgctc ctggtgaaaa tgatattaaa caaagtaaat atcataatca
    16321 tgctatacaa atattagaag gtttaggtgg tcaagagaat attgttaatt taaccaattg
    16381 tgcaacaagg ttacgtctag agttaaaaga cacatctatt attgatcaac agaagattaa
    16441 aaacgccggt gcagttggtg ttactgttaa tggcaaacat tcaactcaag ttattgttgg
    16501 tacacacgtt cagcaggttg ctgatgaaat tgaaaaacat ttataaaatt tagacagcta
    16561 aagcgcaaat ccaactcaat agggtttgcg ctttttttag tcaaatagta gaatgattta
    16621 tatctgaatt ttcagaaatg gagtggggat tgtgaagaaa attttatttg atgttgatgg
    16681 cgttttttta agtgaagaac gttgtttcga tgtctctgca ttaacggtat atgaattatt
    16741 aatggataag tgttatttag gtcttcattc acatatagat tgggaaactt tgactgataa
    16801 cgatatacaa gacattagga atcgaatttt tcaaaaagat aagattttaa acaaattaaa
    16861 atcgctaggg ctgaattcaa attgggatat gttatttatc gtttttagta ttcatttaat
    16921 tgatattttg aaaaagttat cacatgatga aattgaggca ttcatgtatc gagatgagcc
    16981 tgtcgaattg aagttgcaaa atataagcac aaacatagca gaccgtttta atttaaatga
    17041 acaactacct ttacaatttt tggataatgt aaaagttggt aaaaataata tttatgctgc
    17101 tttggaagag tttgcaacaa cagagttaca tgtttcggat gctaatttat ttagtttgaa
    17161 gggtgcatta tggacgttag cgcaagaagt ttatcaagaa tggtatttag gatcgaagtt
    17221 gtatgaagag gttgaaaaga aaatagcacg aactactttt aagacaggtt atatttatca
    17281 agaaattatt ttgagaccgg tagatgaagt taaggtactt ctgaatgatt taaaaggtgc
    17341 tggtttcgaa ttaggtatag caacaggtcg tccttatact gagactgttg tgccatttga
    17401 aaatttagga tttttaccgt attttgaagc tgattttatt gcaacagcaa gtgatgtttt
    17461 agaagcagag aatatgtatc cgcaagcacg accattagga aagccgaatc cttttagtta
    17521 tatcgcagct ttaaatggta ataaacgcga taaatatgaa tcttatatca ataagcaaga
    17581 taacattgta aataaagatg acgtatttat agtaggcgat tcgttagctg acttattaag
    17641 tgctcaaaaa ataggtgcaa cgtttattgg aacattatca ggtttaaaag gaaaggatgc
    17701 tgcaggtgag ttagaagcgc atcatgccga ctatgttatt aatcatttag gtgaacttag
    17761 aggtgtacta gataatttgt aatttgattg ttgtttgaca gcataacttg aagtgaatga
    17821 ttgaaccaaa ggtttcatat tgagttacaa tgaaattaat attaaaaaaa tgccaagaag
    17881 caatggaagt aatccaatgt cttcttggca ttttgaattt atataaattg tttatgactg
    17941 taccgtcaat tcagttgtga aaatttgatt gtattcacca acttgtttaa gttcatcaat
    18001 tatattgttt gaaacaggtt gatcaacgga taaaatcatt agcgcatctc cgcccgcttc
    18061 agttctacct aaagtcatag atgcaatgtt gatattgtat ttacctaaca atgcgccagt
    18121 tttacctacc atgcctggtg tatcattatg atatgacaca atttgatatt gatttggctt
    18181 taagtataca gaaaaattat taattctaac aattcttgga ccgaaacctg taaagacaga
    18241 agcaccaact ttaacggaat cgctatcgtt tgataattct acctctaagt agttactaaa
    18301 acctgtctct gctttattat tttcaatatt taatgtcact tgttgttgat ttaacaacat
    18361 tagagcattg ataatattga cttcaggacc aagatcatct tttaaaatat gagtaataat
    18421 tgtacgtgtt aataaactac tatcaataga ggctaagtcg ccaccgtagg taattttaat
    18481 agagcttggt gcattgtaca ttaattgaat agctaattca ccaactgttt ggcttaaatt
    18541 gatgaatgat tttacagtat catcaatatt gcttaagtcc attttaggtg cattcactgc
    18601 atgcgttaca gtaccatcaa ttaaaatttc gatgatttca tttgaaacag aaattgccac
    18661 tttttcttga gcttcgactg ttgaagcacc caaatgaggt gtaacaataa ttttatcatg
    18721 tgcaacaaga ggcgaatcag ttgcaggttc atgttcaaac acatcgatag ctgctcgact
    18781 aatttgtcct tcgtctaatg cttttattag tgccttttca tcaataatac caccacgtgc
    18841 cacattgatt atttgcaaac taggttttgc tttggcaaaa aagtcagcat taattaagcc
    18901 ttttgtttta ggtgttagtg gtgtatgcaa tgtaacgaaa tcagaatgtt gggcaatctc
    18961 atcaactgtt gccttcgtaa tgcttaaaga ttttgctttt tcatccgtta agtaagggtc
    19021 aaaagctagt attttcatac cgaagctttg tgcacgttta gcaacaccta aaccaattct
    19081 accagcacca atgacaccta atgttttatg ataaagctca gtacctttaa atgcatttcg
    19141 attccattct ttatttgtaa gtgattggtg tgcttgcgga atatttcgag ccattgataa
    19201 taacattgct agtgaatgtt cagtagctga aatcgtatta ccatctgggg cattaataac
    19261 taaaatacct tttaatgttg cagcatcaat attaatatta tctacaccaa caccggcgcg
    19321 tgcgattact tttaaagaat cagcagcatt tatgatattt tcagtaactg tagtttgact
    19381 acgaacgatt aaagcatggt atgaaggtat aattttgatt aatgcttctt cggacaaacc
    19441 agtttgaata tctacattga attgttcgtg atctaataat gctttgatac catcttttga
    19501 tatgggatct gcaacgagta cattaaattg cttcatgaat aacctccata tattttgata
    19561 tgcctttacc gatatagtta actttacggt gttcagttaa aataatttct aaagcagata
    19621 ctaccgataa aatatcgaaa ggactaattt tccccatatg accaattctt aaaatttgac
    19681 ctttaagatg gccttgacca ccagcaattg ttattttaaa gcgattttta agttcatctt
    19741 tgattatttt cacttcatca tttgtgttag gtttgaatgc cgtaaccgtt ggagatgcat
    19801 ctttatcatt gactaataaa gttaaatcta atgcctttaa ggcgcttctt aatgcatttc
    19861 taattgcata gtgtcgtgct attacgtgat tgaaaccttc tgcttttacg gtttcaacgt
    19921 atgcatttac acctctaaat aaagacacat ttggtgtgaa cggtgtagaa ttgtcagctt
    19981 gtgacgaaat gtatttattt aagtctagat aaaattttgg tgtagttact tctttgaaac
    20041 gttcttttgc acggtggcta taagctacaa aagctaatcc tggaggtaac ataattgctt
    20101 tttgactacc agaaacaagt acatcaattt tatctttgtt aatgtcaaca tcaacagcac
    20161 caatgcaact tacgccgtca actacaaaat aaatattact attaaattga ttgatggcat
    20221 ttcctaactc gtgaataggg tgtagcactg tcgtagatgt ttcgcaatat tgactaaata
    20281 ctgctttaac ttcaacattt aaagttgaaa ggtaattgat gaaatctttg acatctacag
    20341 cttctcccca tgttacgtca taaatatgta catttttgta ataagtttgt gcaatttgtt
    20401 taaatcggtt accaaaggca cctgaaacaa tgacaacgaa gtgatcttca gggtttacaa
    20461 tgttcaacat actagcttct aagacgcttg taccgctaga tgttaatata agtacatcat
    20521 tttgactccc aaatattggc tttagacctt gaaatgcttg ttgagcgatg tcttcaaaat
    20581 ctttagaacg atgaccaacc ataggtgctt gaatttctct cataatggca tcaggaacag
    20641 gggttgggcc aggtgttaat aacaacggtt gatgataata catttatatg cctcctataa
    20701 ataagatttc actattttag caaattttct gaaaatttaa aagcctaaaa aaagtgataa
    20761 cgctatattg tcgaacaaat gttatcactt aacgatttta tttggcctga atgatgggat
    20821 agatttttat gtccacatta tttcttacag catttgaaat catgcaatta ttatctgcaa
    20881 ttgttatcaa ttttggtaat cgcttttcta attgtgctat ttgatcactt ggaatttgaa
    20941 tgtgaggatg gtgcacaatt tttgacatac tgaattttcc attatttaaa caagctgttc
    21001 caatcgattg ttgttcaatt gaaatatctg tgaactttgc acgttcaaga gtagctgcta
    21061 atgagatgat ataacatgat gacgcggctg atactaacat ttcatcggga tttgttccta
    21121 taccaacacc acctaaagaa gcaggtattg aaatattctc tgaaagtatg tcgccttgaa
    21181 cgtttccgac attgttacga ccaccttgcc aagaagtttt gactttaaag tcatgttgat
    21241 gcaattgctt taacctccaa tataattgtg atagtttaat tttagaatag tttatcaaag
    21301 tttaacagaa aggtgactta ttaatgactc tgaataaact gaaagatgaa ttacaaattg
    21361 tttcgcatcg tggattgccg agtgattttc ctgaaaatac aatggtcggt tatcgagagg
    21421 taatggggct caatgttgct atgttagaaa tagatgttca tttgaccaaa gaccaacatt
    21481 ttgttgtgat acatgatgaa acaattgata gaacatcgga tggtaagggg cgtattgctg
    21541 attacacatt atcgcaatta aaatcatttg attttggtag ttataaagat gttgctttta
    21601 agggagaacg aatacccacg ttagatgaag tactttcatt atgcttaaag tttgataaaa
    21661 agctattgat tgaattgaaa agtccgaatt tatatccagg aatagaatgt aaattgctgg
    21721 catttttgga agaaaagaaa gtcgatgcga cacaagtagt tatacaatct tttgatatcg
    21781 aatgcatcga aaagttaaac acattaggca gtatatatga attaggtgtg ctttgtagta
    21841 aacgtaagta ttggtataaa aagccaaact tttcaagaat tgctcaaatt gctagttatg
    21901 ttaatcctaa ttatgcatta gtgaccagaa aatttgttga tgaagcacat catcatcaat
    21961 tacaggttat gccatacaca gttaataaat tgaaaactgg agaaaaatta agacaaatag
    22021 gtgtagatgg attaattaca gatgatccga aattatttat taagtaagac gatgaatggt
    22081 tatttgatag agggataagg attcgtatat cgcaattgat atttttaaaa agaaatgatg
    22141 aaatttgtag ataattcaac aaataagact tgtaaattct aagttaattg taaaataggg
    22201 tagctcgata taatcttgaa tctatcattt ttaaattaat aaaaaaaaca cccacaattg
    22261 tgggtgtttg tattgaacgg tattatcttg agtagtactc aacgattaat tgttcgttaa
    22321 tttcagcagg taattcgcta cgttctggta aacgtacgaa agtaccagtt aagctgtcag
    22381 catcaaagtt taagtactca ggtacgaaat tgttgatttc aactgattca acgatgacgt
    22441 ttaatttttg agatttttca cgaactgaaa ttgtttgact aggtttaaca gaataagatg
    22501 gaatatcaac acgtttacca tctactaaga tatgaccgtg gttaactaat tgacgtgctt
    22561 gacgacgagt acgagctaaa cctaatgaat aaacaacagc gtctaaacga cttgctaata
    22621 agatcatgaa gttttcaccg tgtacaccga attttttacc agcgatgtca aatgtgttac
    22681 ggaattgtct ttcagtcatt ccatataagt aacgtaattt ttgtttttca cgtaattgta
    22741 aaccatattc tgataatttt ttacgttggt ttggaccatg ttgtcctggt gcgtaaggac
    22801 gtttttctaa ttctttacca gtaccgctta aagagatacc taaacgacga gattttttcc
    22861 agtttgaacc tctgaatcga gccataataa gactcctcct ttaattcttt tttgttgtta
    22921 tgaataaaca aaaaagagtg ttgcatgctt actaagatat attgttttat gtgtcttcac
    22981 cttatagcga cagttacgtg acacgtccgc attgggaaca acatagcgct caataatata
    23041 caactgctac tttcgttaat tcacacaaat tttattgtaa cattttcctg ctaactgtca
    23101 acactttatt gaaaatgctg atgtcctttt atgcgagttg cttttcaata attttaacaa
    23161 ttgcttcaag atgttccttg tcattgtcat caaatcgatc cgttataggg gcatcgatat
    23221 ctaagacacc gataatttta tcatctttaa aaattggaac gacaatttca gacttactat
    23281 tagcatcaca agcgatatgt cctttgaatt gatgaacatc agctacaact tgtgtacgac
    23341 gttctgaaac ggctgtacca catacacctt ttccaattgg aatgtggaca caagcggggt
    23401 gtccttggaa aggtccaagt ataagttcat tttgttccaa taagtaaaag ccgacccaat
    23461 taatttgatc tagattatca tttaataagg cagacatgtt acttaaaata gcaatcatgt
    23521 gatgttcatc ttcaataagg cttgctgctt gtttctttaa taatgtgtag tttgttgggt
    23581 taattgttgt catatttaaa acctcttatt ttcatagatt ttataatagt atagaatttc
    23641 gaaaatatta tggcaataaa ttcattttat aatttatcct taagcaaaat tacgttataa
    23701 tagtaatgat aaattaggag gagaagcata tggtgttata tatcattttg gcaataattg
    23761 tgattatatt gattgctgta ggtgtattat tctatttacg ttcaaataaa agacaaataa
    23821 tagaaaaagc aatcgaacgt aaaaatgaaa ttgaaacgtt accttttgat caaaaccttg
    23881 cacaattatc taagttgaat ttaaaaggtg aaacaaaaac gaaatacgat gcaatgaaaa
    23941 aggacaacgt agaaagtaca aataagtatc tagctcctgt ggaagaaaaa atccataatg
    24001 ctgaggcttt attagataaa tttagtttca acgcatctca atgtgaaatt gatgatgcaa
    24061 atgagttgat ggatagttac gaacaaagct atcagcaaca attagaagat gtaaatgaaa
    24121 ttattgcgtt atacaaagat aatgatgaat tatatgacaa atgtaaggtt gattatcgtg
    24181 aaatgaaacg tgatgtttta gcaaatcgtc atcaatttgg tgaggcagca agtcttcttg
    24241 aaactgaaat tgaaaaattc gagccaaggt tagagcaata tgaagtacta aaagctgatg
    24301 gtaattatgt acaagcgcac aaccatatag ctgccttgaa tgaacaaatg aaacagctaa
    24361 gatcttatat ggaagaaata ccagaattaa ttagagaaac tcaaaaagaa ttacctggtc
    24421 aattccaaga tttaaaatat ggttgccgtg atcttaaagt tgaagggtat gatctggatc
    24481 acgtaaaagt agacagtaca ttacaaagct taaaaacaga gcttagtttc gttgaaccat
    24541 taattagtcg cttagaatta gaagaagcta atgataaact agctaatatc aatgataagt
    24601 tagatgacat gtatgattta attgaacatg aagttaaagc taaaaatgat gtcgaagaaa
    24661 caaaagatat cattacggat aacttattca aagcaaaaga catgaattat acattgcaaa
    24721 cagaaattga atatgtacgt gaaaactact atataaatga atctgatgct cagagtgttc
    24781 gtcaatttga aaatgaaatt caaagtttaa tttctgtata tgatgatatt ttaaaagaaa
    24841 tgtctaaatc tgctgtacga tatagcgagg ttcaggataa tttacaatat ttagaagatc
    24901 atgtcacagt tattaatgac aaacaagaaa agctacaaaa tcatctgatt caattgcgtg
    24961 aagatgaagc agaagcagaa gacaatctgt tacgagtaca atcgaagaaa gaagaagtgt
    25021 atcgtcgatt acttgcttct aacttaacaa gcgttcctga aaggtttatc atcatgaaaa
    25081 atgaaattga tcatgaagtt cgtgatgtta acgaacaatt tagtgaacgt ccaatacacg
    25141 ttaaacagtt aaaagataaa gtgtctaaaa ttgtgattca aatgaataca tttgaagatg
    25201 aagcaaatga tgttcttgtt aatgctgttt atgcagagaa attaattcaa tatggaaata
    25261 gatatcgtaa ggactatagc aatgttgata agagcttaaa tgaagctgaa cgattattta
    25321 aaaataatcg ctataagcgt gcgattgaaa ttgcagagca agctcttgaa agtgttgagc
    25381 caggtgtcac taaacatatt gaagaagaag ttattaagca atagaaaata gtatgtagtt
    25441 atacttaaat aatatgagct ctctgtcaaa ttggactgat gagtttaata attgaagtta
    25501 gccaacgata cgttgtctag cttctttttt tatatggata tatgaaaggg acaataaata
    25561 taaatagcaa ttgtttaaag ataaacgtaa tcaaatgtga agtttaaatt aatataagta
    25621 gtgaaaaaag catcgtgcta tgcattgagt gaaatagttc aattcttatt aaaatgggtg
    25681 gaaaaatgat taatttttgt tatagccttt attttttatg tgataatagt ataatgcttg
    25741 agtaaaaaca attttaaaag attgatgatt aatgaaagag aagtgtttat tttgatatat
    25801 ctagataatg cggcaacgac gaaagcattt gaagaagtgt tagatactta tttaaaagta
    25861 aatcaatcaa tgtattataa tccgaatagt ccgcataaag ctggtttgca agcagatcaa
    25921 ttattacagc aagcaaaagt tcaaataaat gatatggtca atactaatat gacatatgat
    25981 gttgttttca ctagtggtgc aacggaatct aacaatatcg cgcttaaagg tgtggcttat
    26041 cgtaaatttg atacagcaag agaaataatt acatctgttt tagaacatcc gtctgtttta
    26101 gaagttgtca gatatttgga agagcgcgaa ggctttaaag ttaaatatgt tgatgtaaca
    26161 aaagatggca cgattgattt gcaacatttt aacgatttaa tgtccgataa agtcggttta
    26221 gtaacatgta tgtatgtaaa taatgtaaca ggtcaaattc aacccattcc acaaatggct
    26281 gaagttataa aaaattatcc taaggcacat tttcatgtag atgcagttca agcattcggc
    26341 aaaatttcaa tggatttcaa taacgtagat agtattagtt taagtggcca caaatttaat
    26401 gggttaaaag gtcaaggcgt cttacttgtt aatcatattc aaaatattga gccaatcatt
    26461 caaggtggcg gtcaagagta tggtgttaga agtggtacag tcaatttacc caatgatatt
    26521 gctatggtta aagcgatgaa aatggctaat gaaaaccttg aagcattgaa tgcatttgtt
    26581 actgagttaa ataatgacgt ccgtcaattt ttaagccaat atcaaggtgt ttatattaat
    26641 tcatcaactt caggttcacc attcgtttta aatattagtt ttcctggcgt aaaaggtgaa
    26701 gtattagtta atgctttttc aaaatatgac attatgatat ctacgacgag tgcttgttca
    26761 tctaaacgta ataaattaaa tgaagtattg gctgcaatgg gattatcaga caaatctatt
    26821 gaaggtagta taagattatc atttggagct actacaacta aagaagatat agcgaggttt
    26881 aaagaaatat ttatcatcat ttatgaggaa attaaggagt tgctaaaata atgaagtatg
    26941 atcacttgct tgttagatac ggggagttaa cattaaaggg ttcaaataga aaaaaatttg
    27001 taaatcaatt aagaaataat gtaaataagt cattaaaagg acttgatgga tttgtcgtta
    27061 aaggcaaacg agatcgtatg tatattgaac ttgaagacca tgcagatata aatgaaataa
    27121 caaatcgatt atcaaaaatt ttcggtatta aatctattag tccagtatta aaagtagaaa
    27181 aatcaataga ggcaatgagt gcagcgacaa ttaaatttgc gcagcaattt gaagaaaaca
    27241 gcacttttaa aattgatgtg aagcgtgccg ataaaaattt cccaatggat acgtatgaat
    27301 tacaacgtga attgggtggt accgtattga aacaaattga aaatgtttct gttaatgtca
    27361 aacgtccaga tcatgaaatt cgagtggaag ttagattaga tgcaatttat atgtatgaag
    27421 aagttgttcc gggttcaggt ggattaccag ttggtactgg tggtaagacg ttactaatgc
    27481 tttcaggcgg tatagactca ccagttgctg ggatggaagt gatgagacgt ggcgtaacaa
    27541 ttgaagcgat tcatttccat agtccaccat ttacaagtga tcaagcaaaa gaaaaagtta
    27601 ttgaattgac acgtatttta gctgaacgtg ttggaccaat taaattgcat attgtaccat
    27661 ttacagaatt gcaaaaacag gtaaataaag ttgtacatcc aagatataca atgacttcaa
    27721 cgagacgtat gatgatgcgt gttgctgata aattagtaca tcaaataggc gcttatgcca
    27781 tcgttaatgg tgaaaattta ggtcaagttg ccagtcaaac acttcatagt atgtatgcaa
    27841 ttaataatgt aacttctacc cctgtattac gtcctttatt aacttacgat aaagaagaaa
    27901 ttattattaa atcgaaagaa attggtacat ttgaaacatc tattcaacct tttgaagatt
    27961 gttgcacaat tttcacccct aaaaatccag taaccgaacc aaactttgat aaggtagttc
    28021 aatatgaaag tgtctttgat tttgaagaga tgattaatcg tgctgttgaa aatattgaaa
    28081 cacttgaaat aactagtgat tataaaacta ttaaagaaca gcaaacaaac caattaataa
    28141 acgacttttt ataaataaaa tcttagagtg aatttaaaca taaggggatg ttaaactatg
    28201 gatttgaatt taacgatgat tataatcata attttatttg gttttatcgc ggcgtttata
    28261 gattcggttg tagggggtgg tggtttaatt tctacgccag cattattagc aatcggtcta
    28321 ccaccatctg tggctttagg tacaaataaa ttggcaagtt catttggttc tttaactagt
    28381 gcgataaagt ttataaggtc cggtaaagtg gacttatatg ttgttgccaa attatttggt
    28441 tttgtatttt tggcatctgc atgtggcgca tatattgcaa ccatggttcc gtcacaaata
    28501 ttgaaacctt taatcattat tgcactttcg tcggtgttta tattcacatt acttaaaaaa
    28561 gattggggca atacacgtac gtttactcaa tttacattta agaaagccat actatttgca
    28621 gcacttttta ttttaatcgg cttttatgat ggatttgtag gtggaggaac aggttcattt
    28681 atgctttttg tattgttaat ctttggtttt gattttttaa gcgctgcagg aaatgctaaa
    28741 gttttgaatt ttgcttctaa tataggtgcg cttgtattat ttatggtatt aggacaagta
    28801 gattatgtaa taggtttagt tatggctgcg agtatgattg tagggtcata tgctggggct
    28861 cattttgcta tcaaacaagg tgttggctat gtgaaagtat tatttattat tattactgcg
    28921 attttaattt taaaaaatgc ttttgattat attcaacaat ttgtataaaa ttaaattcgc
    28981 ttttgattaa agcatgaaaa ttgtaatcaa accataaatt gtcgtatgat gtagttagaa
    29041 ttttaaaatg caggaggtca agtatatgac tgaaataaca ttcaaaggtg gaccaatcca
    29101 cttaaaaggt caacaaatta atgaaggtga ttttgcacct gattttacag tgttagataa
    29161 tgacttaaat caagtaacat tagcagatta tgctggtaaa aagaaattaa ttagtgtggt
    29221 accatcaatt gatacaggtg tttgtgatca gcagactcgc aaattcaact ctgaagcttc
    29281 taaagaagag gggattgtgc ttacaatctc agcagactta ccattcgcac aaaaaagatg
    29341 gtgcgcttca gcaggtttag acaatgtcat tacattaagt gaccaccgtg acttatcatt
    29401 tggtgaaaac tatggcgttg ttatggaaga acttcgctta ttagctcgtg cagtatttgt
    29461 attagatgca gataataaag ttgtttataa agaaatcgtt agtgaaggta ctgatttccc
    29521 agattttgat gctgctttag ctgcatacaa aaatatttaa tcattaaaga gataaatctt
    29581 aaaatgtata catcgtgtcc atcgttgtca acagcattaa aatagaattg ttttctatga
    29641 ttgctaagac ctatgggcac tttttattgg agagggacga atatggcaga acaacaaaca
    29701 attatggaac gcttgtttca ttcattagat gaaaaagcta aaacattaaa taatgaaaat
    29761 ggccaaagtt ttattgaaaa tcttgggcta gcaatggaac aagtttatac aaatgaacga
    29821 ggattgttag aacaatcgac gttacaagat cgacgtaaag cattccaatt tgcatattta
    29881 agtttaatgc aggaagaaaa gatacaagca aatcatcaaa ttacaccaga ttcaattgga
    29941 ttgatattag gatttttagt tgagcgattt atgaacaatc aagaagaatt acatattgtt
    30001 gatattgcaa gtggtaccgg tcatttaagt gctactgtaa aagaagtgtt acctgaaatt
    30061 gcggttatgc atcatttaat tgaagttgat ccagttttat cacgtgttag tgtacattta
    30121 gcaaacttct tagaaattcc tttcgatgtg tatcctcaag atgccattat gccactaccg
    30181 ttagaagaag cagatatcgt tattggtgat tttccagtag gctattatcc aattgatgaa
    30241 agaagcaggg actttaagct aggtttcgaa gaaggacata gttattcaca ttatttatta
    30301 atagaacaag caataaatgc attaaaagat gctggatatg cctttctagt ggtaccaagt
    30361 aatattttta caggtgaaca tgtaaaacag cttgaaaaat atattgcaac agagacagaa
    30421 atgcaagcat ttttaaattt accaccaact ttatttaaaa atgaaaaagc gcgaaaatct
    30481 atattaattt tacaaaagaa aaaatcgggt gaaacaaagt cagttgaagt attattggca
    30541 aatattcctg atttcaaaaa tccttcacaa tttcaaggat ttatgacaga gttaaatcag
    30601 tggatggaca caaatcgtcc taaaaaataa tctgtaatag tatagtcata aaactgtatg
    30661 aatgataaaa tgaaaatgaa atagtataaa aagcaaacga tggaggatca ttacttatgt
    30721 caaaattaat cttggctatc aacgctggta gttcatcatt aaagtttcaa ttaatcagaa
    30781 tgcctgaaga ggaattagta acaaaaggat tagtagaaag aatcggatta aaagattcta
    30841 tttttacaat tgaagtagac ggggaaaaag ttaaaacagt acaagatatc aaggatcatg
    30901 ttgaagcagt tgatatcatg ttagacgcat ttaaaaaaca taatatcatt aatgatatta
    30961 acgatatcga tggaactggt caccgtgttg tacatggtgg tgaaaaattc cctgaatcag
    31021 cagcaattac tgatgaagtt gaaaaagaaa ttgaagaatt aagcgaatta gcaccgcttc
    31081 acaacccagc taacttgatg ggtattcgtg ctttccgtaa attattaccg aatatccctc
    31141 atgtggcgat ttttgataca gcattccatc aaacaatgcc tgaaaaagca tatctatata
    31201 gcttgccata tcattattat aaagattacg gtattcgtaa gtatggcttc catggtacaa
    31261 gtcataaatt tgtgtcacaa agagcggcag aaatgttaga taaaccaatt gaagatttac
    31321 gcattatttc ttgtcatatt ggtaacggtg catcaattgc agctatcgat ggcggtaaat
    31381 ctattgatac atcaatgggc ttcacaccac ttgctggtgt aacaatgggt actcgttctg
    31441 gtaatattga ccctgcttta attccattta ttatggaaaa aactggcaaa actgctgaac
    31501 aagtattgga aattttaaac aaagaatcag gtttattagg attatcaggt acatctagtg
    31561 atttacgtga cttatcagaa gaagctgaat caggtaaagc acgttcacaa atggctttag
    31621 atgtatttgc ttctaaaatt cacaaatata ttggatcata tgctgcgaga atgcatggtg
    31681 ttgatgtaat cgtatttact gcaggtattg gtgaaaattc agtggaaatt cgtgcaaaag
    31741 ttcttgaagg tttagaattt atgggcgttt actgggatcc taagaaaaat gaaaacttac
    31801 aacgtggtaa agaaggattt attaactatc cgcattcacc tgttaaagtt gtcgttatac
    31861 cgacagacga agaaagtatg attgctcgtg atgttatgac atacggtggt ctaaaataat
    31921 tttttaataa atattaagcg tctatctccg ctacaattat aagtaggagg tagacgcttt
    31981 ttgtattgtt tgaaaattaa ctagtgtaat gtcttttttc acgaatatct taaaaaaatt
    32041 aagtagtcga agaagattat gacaacatta ttaagtgagt tttatttata aacaataaaa
    32101 aaccaagtgc agaaagttgc acttggttca taatgatcaa gtagttatct aatgatcatc
    32161 tatatttaat tttgatattt ttcacgtaat tgagttgttg caacttgtgg ttggaagtct
    32221 gctggtaact cttcagtacg aacaactaac acgtcacatg gagcatgacg aacgatagat
    32281 tctgatacag aaccaacaat aaatctttcc acggcattta agcctgatgt accactcata
    32341 atcaagtctg cattaatttc atgtgcaagc tttttaggaa tgatagattt aggagagcca
    32401 aactctagac gcgtttctac atctttaaca ccagcgttag tagctacttc tttataacca
    32461 tttaataatt cttctgcaaa atgcttagat ttttcagtaa attgagcatc ataaacttca
    32521 taagaagaat acgttcttga atcaattaca ttcacaattg ttaacttcgc atcgttacgt
    32581 ttagcaacac caactgctct gttaaatgcc cattccgctt catgtgaacc gtcaactgcg
    32641 attaaaatat ttttgtaagt aatcatagta agtgcctcct tgttctttct taattttatt
    32701 ttaccacaat tttcggaaaa gtcagtgtaa taacctttac aattttgtgg atttttgatt
    32761 gcgctttcaa aatgttcgcg ttaagatgat tatggaggtg gatggtatga aaattggtat
    32821 accaagggag attaaaaata atgaaaatcg tgttggttta tcaccaagtg gtgtgcacgc
    32881 tttagtagaa agtggacata ctgttttagt ggaaacaaat gcgggttcag gatcgttctt
    32941 tgaagatgta gattacaaag aagcaggtgc tgagattgtt gctgaacaag caaaagtttg
    33001 ggatgtggat atggttatta aagttaaaga acctcttgaa tcagaatatc catattttaa
    33061 agaaggacta gtattattta cttatcttca tctagcgaat gaagaaaaat taacacaagc
    33121 tttgatagat agaaaagtaa ttagtattgc atatgagact gtgcagttac cagaccgatc
    33181 tttaccatta ttatcaccaa tgagtgaggt agcaggaaga atgtcagctc aagttggcgc
    33241 agagttccta caaaaactta atggtggtat gggaattcta ctaggtggcg ttccaggagt
    33301 acctaaaggt aaagtaacta ttatcggtgg tggtcaagca ggaacaaatg cagctaaaat
    33361 tgcactagga ctaggtgcag atgttacgat tttagatgtt aatccaaagc gtttacaaca
    33421 attagatgat ttattcggtg gacgtgtaca tacaattatg tcaaatccgt tgaatattga
    33481 gttgtatgtt aaacaaagtg atttagtaat aggtgcagtt ttaattccag gtgctaaagc
    33541 gccaagactt gtaacagaag acatgattaa acaaatgaaa aatgggtcag ttattattga
    33601 cattgctatt gatcaaggcg gtatttttga aacaactgat aaaattacga cacatgatga
    33661 tcctacatat atcaaacatg gtgtggttca ttatgcagtt gcaaatatgc cgggtgcagt
    33721 accgcgtact tcgacgttag ctttaaataa tgctacgcta ccttatgcgc tcatgctagc
    33781 taataaaggg tatagagaag catttaaatc aaatcaacca ttatcattag gtttaaatac
    33841 ttacaaaggt cacgtaacca ataaaggcgt tgcagaggca tttgaaatgg aatataaatc
    33901 tgtagaagaa gcattacact tataatttaa atttgatgaa aagggaatga attacagcgt
    33961 attaaagaat gacgttatca tctccttggt tattaaaatt aaataatgat aaattaaagc
    34021 tagaaacaat gcgaagaaat catcgaacat gaatgatatt gattcattac atgttactga
    34081 tgaacatacc tgcatatgtg tttctagctt gtttgttaca tattgcattt agacttggac
    34141 aaaaatttat gtcctaggcg ctacaatgtt atattggcag tgtttgactg aatgagaatg
    34201 cgctagtaac aagcttttca tttttgtccc actcttattt ttcgtaatgt gttaatactt
    34261 cgtatccttc attagtgaca agtatgtcat cttcaattct tacacctgca acacctggta
    34321 cataaatacc tggttcgatt gtaatgacca tgccagcttc taacaaatta gaattagtac
    34381 ttgaaacatc ttgatattca tgttcctgta atcctagacc atgacctaag cgatgaggga
    34441 aatattcacc ataacctttt tctgaaataa tatttctagc gatatgatcg atatctttta
    34501 aaggtattcc aggtttaatt gcttggattg cagatgtttc tgcttctaat acaatattat
    34561 aaatttcttg tgcttcttgg ctaggttcac caaatttaat ggtacgtgtc atatcgctac
    34621 aataatgctc ataaattaca cctaaatcaa atagtacata ctcattgctt tttaatctgc
    34681 gatctcctgg tgtgccatga ggtgatgcgg catgatctcc aaataaaacc atcgtatcaa
    34741 aactcatttc attgacgcca tattgtttga ttgtttgctc aatatggttg actacttcac
    34801 gttcagttac actttctttt aaataagaaa cacctatttc gatacactta tctgctaact
    34861 cagcagcttt acgtattttg ttaatttcat cttcagattt aatatttctc aattgtttga
    34921 ttgttaaatc aacatctccg aatgaattga cattgaaacc agagattaat tgtttttggc
    34981 gtgctactgt taagtgctcg ctttcaatta gtaatttatt gattgtttga ggataaagtg
    35041 aaaaaggatt ttcagtgtct aaatatccaa cgatttcacc tgtgaaaggt gatgctttga
    35101 cttcttcgac ttccattttt ggacaaaata gtacttgttt accatctttc ttaatcaata
    35161 atgcaaataa tctttcatgg ggttcgctac ggtatccagt aaaataatat acattcaacg
    35221 gtgttgtaat ccatgctgca tcagcttgtt gattgttcag ttcgtctatt atttttgata
    35281 tttttgtcat tttataaaca ctcctcaaca taatattact tacattttat gctaattatt
    35341 attagatttc aaaatcgagc taatacatta actagatttt caatttttaa aatttgagaa
    35401 tttgttataa ttaagaggca aaagggtact aaatttatat agcaattgat aggaggatgc
    35461 tcaaatgaag ttatcatttc acggtcaatc aacgatttat ttagaaggga acaacaaaaa
    35521 ggttatcgta gatccgttta tttcaaataa ccctaagtgt gatttgaata ttgaaacagt
    35581 gcaggtagac tatatcgttt taacgcacgg tcattttgat cattttggtg atgttgtaga
    35641 actagcaaag aaaacagaag caacagtaat tggaagtgca gaaatggctg actatctttc
    35701 ttcatatcac ggtgttgaaa atgttcatgg tatgaatatt ggtggtaaag caaactttga
    35761 tttcggaagc gttaaatttg ttcaagcatt tcatagttca agttttacac atgaaaatgg
    35821 tattccagtt tatttaggta tgccaatggg tatcgtgttt gaagttgaag gaaaaacaat
    35881 ttatcatact ggtgatacag gtctgtttag tgacatgtca cttattgcga aacgtcatcc
    35941 agttgatgta tgttttgtac caattggaga taattttacg atgggtattg atgatgcaag
    36001 ttatgctatt aatgaattta ttaaaccaaa aatttctgta ccgattcatt atgatacatt
    36061 cccattaatc gaacaagatc cacaacaatt taaagatgct gttaatgtag gggacgtgca
    36121 aattttaaaa ccaggtgaat ctgttcagtt ttaaatagaa tatagatttt acactaaaaa
    36181 acacctcgct aatctatgtg gaagttatcg tctgcatatt tttaatgggt gtttttatat
    36241 tctactgatt cagttgcgac gtggacgggg gagacatcag tggttcttta tcatttctga
    36301 cctactctcg taattttagt tttatacaat tagacattga ttataaattt actacgatat
    36361 aaaaaatcca gaaccacagt cataatcatt tcttgtgttt actcagtcgt ttgagactgc
    36421 atcatgattg aaagattatt tgtgattctg gataaaaatt ttatttaacg attaatacag
    36481 ggatagtcgc acgttttgct actttgtggc tgacacttcc aagtacaaat ttctttttgt
    36541 cttctgcttt acggttactt aaaacaacaa tttcgtattt accgctatta gcatgtttta
    36601 atagttcttc ttttgcatta ccacgaacaa ttatttggtc ataatcaata ccataatctt
    36661 ctaaagcttt gcgtgttttt ttcaatcgct tgcttcgttc ttcagtaagt ttgtttaaat
    36721 gaacacctgc tttaattgat gcttgagcat cttgttcgct gattgcgttt aaaactgtta
    36781 cgactgtacc ttcgccagct aatttagaca cttcttttag tgctttttca ttttttaact
    36841 gagtgtctac accaagtaat atatttttat acattagtac atccctcctc acttacattc
    36901 taataaataa taatggattt ttccatttaa aattaaagat tttgtgatct ttgtgtttgt
    36961 tgtatatttt caaaaaataa aataggtgaa acgatcatta tttcatttca cttcaatgct
    37021 ttttatccaa aataaagtta taatgtagtt aatactgttc gaaaagaaga ggtaaagaat
    37081 gacaaaacat gaacaaattt tagattatat tgaatcgtta tctataggct ctaaaatttc
    37141 ggtgcgtaaa atcgctaaat ttttaaacgt atctgaaggg acagcatatc gtgcaattaa
    37201 agatgctgat aaaatgggca tggtcgcaac gatagatcgt gttggtacgg ttcgaatcga
    37261 aaaacgtaat cgtaatgaaa ttgaacattt aacttttaat gaaattgtca atattattga
    37321 tggccaagtg ttaggtggca acaaaggtat cactaagatg gtatctaaat ttgcaattgg
    37381 agcaatggaa ctcaaagata ttttaaagta tattgggcca aagacattat tgattgtcgg
    37441 aaatcgcgaa gatgtgcaga ttgaagcttt aaaaagaggc acggctatat taattactgg
    37501 tggatttaag ccatctaata aagttattga ttttgcgaat gaacatgatt tacctgttct
    37561 atcatcaagt tatgatacat tcctagttgc aaatattatc aacaaagcac tttttaatca
    37621 gaaaatacga aaagatatct taatagttca agatataatg acgccattag atgatttatc
    37681 tgtgctgttt gatacgatga aaatagcaga ttataagcgt atggcgaatc aaactggcca
    37741 tacaagattt ccggtagtca atgagtctta taaattagta ggtattgtaa caagtcgaga
    37801 gatgattaat acgaaggacg acgacgaaat agataaagtg atgacgcgaa atcctattta
    37861 tgtaaatgct atgagtacag tagcaagttg tgcgcatatg atgatttggg aaggtattga
    37921 attgattcct gtcgtttcga gtaataaaaa gacagtgggt gtgattaata gacaagatgt
    37981 tttgaagtct atgcaacttt taggaagaca acctcaaatg ggtgaaacaa ttaacgatca
    38041 aatcgccaaa tatataacaa tgaatcaaga tggaattaca gtagaagttt caccactgtt
    38101 aatcaatcac tacggaacag taagtaaagc agcatttgta agtattatag aagaaacaat
    38161 acaatatgaa atgcgtaaat ttaaaaaagg taatgttatg atagagaact taaatatcgt
    38221 gtatattaaa acagtaccta ttgaatcaca tataacagtg cgctttggca tactagacgt
    38281 cggaagaaac ttcgccaaaa tagaagtgaa catgcatagt caaaacgaca aagtagcgag
    38341 cgctctcgtg atatgccaaa tgtttgacga agtataggaa cttagcagag aaaataggca
    38401 tacgatagat ttcacacaaa tgttggagcc caacttgcat tgtctgtaga atttcttttt
    38461 gaaattctct gtgttggggc cccaccccaa cttgcacatt atagtaagct gacattacgt
    38521 caccttctgt gttggggccc ctgaactaat tagtatgaaa aaatatatat ctaaataatt
    38581 aaatcgggaa aatagtttaa tatggtagaa caatttttat aatttagata ttttgatata
    38641 gactaatgct taaattaagc aatttttcta aaaaagggag aatgaaaatg attagtacga
    38701 tgaatgaaat tatgaaatgt atcgaagaca acgatacaat tattatacac agacatgtta
    38761 gaccagatcc tgatgcatat ggttcacaac ttggtttaaa atactatatt cagcagaaat
    38821 ttccgcaaaa gcaagttttt gcagtgggtg aagcggaatc atcattaagt tttattggag
    38881 agttagataa cattgatgat aaaacatatc aagatgcgct tgtaatagta tgtgatactg
    38941 ccaatgctcc acgaattgac gatgaacgtt atagtacagg tcgtaaactt attaaaatag
    39001 atcatcatcc tgcagttgat cagtatggtg atattaatct agttaatacg aacgcgtcat
    39061 ctacaagtga aatcatttac gatttaatct cacattttaa tgatgaagca attgttaata
    39121 aagatatcgc gagtgtttta taccttggta tcgtcggtga tactgggcga ttccttttta
    39181 acaatacctc agaacatact atggaaattg ctggaaagtt aattgggcat gatattgatc
    39241 ataatgcgct tttaaataaa atgatggaaa aggatccaaa aatgttgccg tttcaaggtt
    39301 atgttttaca acatttcgaa cttatggatg acggattttg ccaagttaaa ataactgaag
    39361 atgtattgga gcaattcggt attcagccaa atgaagcatc tcagtttgtt aatacaattg
    39421 ctgacatcaa aggtttgaaa atatgggtgt ttgcagttga tgagggtagt gaaataagat
    39481 gtcgcttacg ctctaaaggg caattgatta ttaatgatat tgcgcaagat tttggtggcg
    39541 gtggtcatcc gaatgcgtca ggagtttcag tgaacagctg ggatgaattt gagcaacttg
    39601 ctaaagcttt acgcacgaaa cttaactaat agaaaggggc cattcaatca tggtggcata
    39661 tttaaatatt catacggctt atgatttgtt aaattcaagc ttaaaaatag aagatgccgt
    39721 aagacttgct gtgtctgaaa atgttgatgc acttgccata actgacacca atgtattgta
    39781 tggttttcct aaattttatg atacatgtat tgccaataat ataaagccga tttttggtat
    39841 gacaatatac gtgacaaatg gattaaacaa tattgaaacc gttgttttag caaaagataa
    39901 ttatggtctg aaggatttat atcaactatc atcggaaata aaaatgaatg cattagaaca
    39961 tgtgtctttt gaattattaa aacggttttc taacaatatg attatcattt ttaaaaatgt
    40021 tgcagacgaa catcgtgaca ttgttcgagt ttttgattcg catgaagata cgtatttaga
    40081 tcatcgaagt gttttagttc agggtataaa gcacgtatgg attcaagatg tatgttatca
    40141 aacacgacat gatgcagata caatttctgc actcgcagcg ataagagata atacaaaatt
    40201 agacttaata catgatcaag aagattttgg tgcgcatttt ttaactgaaa atgaaattca
    40261 ccaacttgat gtgaatccag aatatttcac acaagctgac aggatagctc aaaagtgtaa
    40321 tgcagaatta aaatatcatc aatctctact tcctcaatat cagacaccta atgatgaatc
    40381 agctaaaaaa tatttgtggc gtgtcttagt tacacaattg aaaaaattag aacttaatta
    40441 tgacgtctat ttagagcgat tgaaatatga gtataaagtt attactaata tgggttttga
    40501 agattatttc ttaatagtaa gtgatttaat ccattatgcg aaaacgaatg atgtgatggt
    40561 aggaccaggt cgtggttcat ctgcagggtc tctggtcagt tatttattgg gaattacaac
    40621 gattgatccg attaaattca atctattatt tgaacgtttt ttaaacccag aacgtgtaac
    40681 aatgcctgat atagatattg actttgaaga tacacgccga gaaaaggtca ttcagtacgt
    40741 ccaagaaaaa tatggcgagc tacatgtatc tggaattgtg actttcggtc atctgcttgc
    40801 aagagcggtt gctagagatg ttggacgaat tatggggttt gatgaagtta cattaaatga
    40861 aatttcaagt ttaatcccac ataaattagg aattacactt gatgaagcat atcaaattga
    40921 cgattttaaa aagtttgtac atcgaaacca tcgacatgaa cgctggttca gtatttgtaa
    40981 aaagttagaa ggtttaccaa gacatacatc tacacatgcg gcaggaatta ttattaatga
    41041 ccatccatta tatgaatatg cccctttaac gaaaggggat acaggattat taacgcaatg
    41101 gacaatgact gaagccgaac gtattggttt attaaaaata gattttctag ggttacgaaa
    41161 tttatcaatt atccatcaaa ttttgactca agtcaaaaaa gatttaggta ttaatattga
    41221 tattgaaaaa attccatttg atgatcaaaa agtgtttgaa ttgttgtcgc aaggagatac
    41281 gactggcata tttcaattag agtctgacgg tgtaagaagt gtattaaaaa aattaaagcc
    41341 ggaacacttt gaggatattg ttgctgtaac ttctttgtat agaccaggtc caatggaaga
    41401 aattccaact tacattacaa gaagacatga tccaagcaaa gttcaatatt tacatcccca
    41461 tttagaacct atattaaaaa atacttacgg tgttattatt tatcaagaac aaattatgca
    41521 aatagcgagc acttttgcaa acttcagtta tggtgaagcg gatattttaa gaagagcaat
    41581 gagtaaaaaa aatagagctg ttcttgaaag tgagcgtcaa cattttatag aaggtacaaa
    41641 gcaaaatggt tatcacgaag acattagtaa gcaaatattt gattttattc tgaaatttgc
    41701 tgattatggt tttcctagag cacatgctgt cagctattct aaaattgcat acattatgag
    41761 ctttttaaaa gtccattatc caaattattt ttacgcaaat attttaagta atgttattgg
    41821 aagtgagaag aaaactgctc aaatgataga agaagcaaaa aaacaaggta tcactatatt
    41881 gccaccgaac attaacgaaa gtcattggtt ttataaacct tcccaagaag gcatttattt
    41941 atcaattggt acaattaaag gtgtaggtta tcaaagtgtg aaagtgattg ttgaagaacg
    42001 ttatcagaac ggcaaattta aagatttctt tgattttgct agacgtatac cgaagagagt
    42061 caaaacgaga aagttacttg aagcactgat tttagtggga gcgtttgatg cttttggtaa
    42121 aacacgttca acgttgttgc aagctattga tcaagtgttg gatggtgatt taaacattga
    42181 acaagatggt tttttatttg atattttaac gccaaaacag atgtatgaag ataaagaaga
    42241 attgcctgat gcacttatta gtcagtatga aaaagaatat ttaggatttt atgtttcgca
    42301 acacccagta gataagaagt ttgttgccaa acaatattta acgatattta aattgagtaa
    42361 cgcgcagaat aataagccta tattagtaca gtttgataaa gttaaacaaa tccgaactaa
    42421 aaatggtcaa aatatggcat tcgtcacatt aaatgatggc atcgaaactt tagatggtgt
    42481 gattttccct aatcagttta aaaagtacga agagttgtta tcacataatg acttatttat
    42541 agttagcggg aaatttgacc ttagaaagca acaacgtcaa ttaattataa atgagattca
    42601 gacattagcc acttttgaag aacaaaaatt agcatttgcc aaacaaatta taattagaaa
    42661 taaatcacaa atagatatgt ttgaagagat gattaaagct acgaaagaga atgctaatga
    42721 tgttgtgtta tccttttatg atgaaacgat taaacaaatg actactttag gctatattaa
    42781 t
//
LOCUS       DAKSQE010000008        40920 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.008, whole genome shotgun sequence.
ACCESSION   DAKSQE010000008 DAKSQE010000000
VERSION     DAKSQE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 40920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40920
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.008"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..169
                     /locus_tag="O0V33_000302"
     CDS             <1..169
                     /locus_tag="O0V33_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HCX8974990.1"
                     /translation="KSNKNPKLLFKGAGNIKGSSVGYKEIQIIFNRNKEESVSCIDSI
                     EFKPSEGDYNE"
     gene            182..1489
                     /locus_tag="O0V33_000303"
     CDS             182..1489
                     /locus_tag="O0V33_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8974991.1"
                     /translation="MRCWQIKKDTEPTSAISFMAYEIENANNHNLSKMAIKEQLDRSA
                     ERGMFPSNLEYVDSYTDSLTGVTTSAFLNKDTGKVTLGMTGTNIQGEAFKKLKEGEFS
                     RQNVTNALETVKDGYADIKILYSPASDQNYRYANTQEFINKIKSKYDIDFITGHSLGG
                     RDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITRFVAENDA
                     LTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLKGYAEENNKSF
                     VKHSNNAISKLASIELLRANMMTANGGGLSSSQQKVLDSLTALTIAQSFSQLIDDEIN
                     QIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNEVLNALDEGQVNESSMVREPEQMI
                     SAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
     gene            1486..1809
                     /locus_tag="O0V33_000304"
     CDS             1486..1809
                     /locus_tag="O0V33_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8974992.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELQNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            1828..2142
                     /locus_tag="O0V33_000305"
     CDS             1828..2142
                     /locus_tag="O0V33_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8974993.1"
                     /translation="MTLIEPDMTLRMPDISTTVETLNLISKMNAQKENIRTVIAPEHK
                     HKYKDIENGLKGEEKVLIEQMAQHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(2431..2691)
                     /locus_tag="O0V33_000306"
     CDS             complement(2431..2691)
                     /locus_tag="O0V33_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8974994.1"
                     /translation="MSTLLYNRDKDISHERESRCINMLFVILVLYIIGIAFILLSVFG
                     SKTEGLSTKHTLYTIGSAIITIAIFISIGYAIQYLTAALYGL"
     gene            2810..4012
                     /locus_tag="O0V33_000307"
     CDS             2810..4012
                     /locus_tag="O0V33_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCX8974995.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKVLSALQPTAKIIKTTNSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLESMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTDLDEGAITKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            complement(4671..4733)
                     /locus_tag="O0V33_000308"
     CDS             complement(4671..4733)
                     /locus_tag="O0V33_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HCX8974996.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(4797..4865)
                     /locus_tag="O0V33_000309"
     CDS             complement(4797..4865)
                     /locus_tag="O0V33_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HCX8974997.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNY"
     gene            complement(4917..4982)
                     /locus_tag="O0V33_000310"
     CDS             complement(4917..4982)
                     /locus_tag="O0V33_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HCX8974998.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            complement(5014..5079)
                     /locus_tag="O0V33_000311"
     CDS             complement(5014..5079)
                     /locus_tag="O0V33_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HCX8974999.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            5598..7082
                     /locus_tag="O0V33_000312"
     CDS             5598..7082
                     /locus_tag="O0V33_000312"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HCX8975000.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGMTLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVSIYIATGEWRVPQHIAHPTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILAWSLLVSWNQMVAF
                     SKGRMARLVGMILISIVTFIYIITHNYFYDVLQNITTHATTPPTISVIISVVILIFGS
                     LLSIWVARHRYSKTFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            7095..9800
                     /locus_tag="O0V33_000313"
     CDS             7095..9800
                     /locus_tag="O0V33_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HCX8975001.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPKMTIESVLNHFSIAQEDYQAYVEGHLLALPGWAGILYYRSQQHHFEQHLLTDYLAI
                     RLVVEQLLVGNEFKSVTKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVTENDNANQS
                     HSVSLNATQAVDENNSELNQVGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRVSLQKIKQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQAGVYIPETTVFAAAEHHTSTDTLAWVYVPDT
                     LSALALDAYESLNDVMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGKLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVVQAPDFVVARLLANNEHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            9962..10324
                     /locus_tag="O0V33_000314"
     CDS             9962..10324
                     /locus_tag="O0V33_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCX8975002.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIIFKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(10553..10969)
                     /locus_tag="O0V33_000315"
     CDS             complement(10553..10969)
                     /locus_tag="O0V33_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975003.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYIYYTEGVGRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
     gene            11007..11681
                     /locus_tag="O0V33_000316"
     CDS             11007..11681
                     /locus_tag="O0V33_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCX8975004.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYSVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISVA
                     AKTMTKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            11718..12452
                     /locus_tag="O0V33_000317"
     CDS             11718..12452
                     /locus_tag="O0V33_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCX8975005.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFITYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSNILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVVKIPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENIIRFFNALT"
     gene            12630..12743
                     /locus_tag="O0V33_000318"
     CDS             12630..12743
                     /locus_tag="O0V33_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975006.1"
                     /translation="MGIVPEVVMWRDVGDMDGVVCGEMLAQVYSVEPEILM"
     gene            12909..14246
                     /locus_tag="O0V33_000319"
     CDS             12909..14246
                     /locus_tag="O0V33_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HCX8975007.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIIIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVVGQLLDKKLLQQYFGKDRFRLFSGWYYLIKYAMPVVIILVFIVQLFS"
     gene            14462..15367
                     /locus_tag="O0V33_000320"
     CDS             14462..15367
                     /locus_tag="O0V33_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCX8975008.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEMAIQEGRVRAGQIIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGADVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QHIDYFVAGIGSGGTYTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERSLVDGIFTIKDQDAFRNVKSLAKHEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKEIFNYEENDYE"
     gene            15360..16502
                     /locus_tag="O0V33_000321"
     CDS             15360..16502
                     /locus_tag="O0V33_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCX8975009.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVIASLENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGVEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTLYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            16793..17818
                     /locus_tag="O0V33_000322"
     CDS             16793..17818
                     /locus_tag="O0V33_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCX8975010.1"
                     /translation="MIEFRQVSKSFHKKKQTIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSDKMMRDIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPDELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGQ
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSPSLIRRLNDKQVGDFKDYKIFVEETQ
                     MTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNMDQQFDGTAINQYFKEKN
                     IQFEEVH"
     gene            17822..18481
                     /locus_tag="O0V33_000323"
     CDS             17822..18481
                     /locus_tag="O0V33_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8975011.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPIINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVVIVQVIQTLGNVLARFIRR
                     H"
     gene            18518..19360
                     /gene="gmpC"
                     /locus_tag="O0V33_000324"
     CDS             18518..19360
                     /gene="gmpC"
                     /locus_tag="O0V33_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HCX8975012.1"
                     /translation="MKRLIGLVIVALVLLAACGSNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGVYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     DITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSDSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
     gene            19694..20698
                     /gene="aaa"
                     /locus_tag="O0V33_000325"
     CDS             19694..20698
                     /gene="aaa"
                     /locus_tag="O0V33_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HCX8975013.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNSTSNSSRPSTNSSGGSYY
                     TVQAGDSLSLIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVTGNASTNSGSTTTTNRG
                     YNTPVFNHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPT
                     VGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH
                     "
     gene            complement(20885..21154)
                     /locus_tag="O0V33_000326"
     CDS             complement(20885..21154)
                     /locus_tag="O0V33_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975014.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            21304..21699
                     /locus_tag="O0V33_000327"
     CDS             21304..21699
                     /locus_tag="O0V33_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCX8975015.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESPVHALL
                     REVKEELNLTLTMDEIEYIGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYDGKHDK"
     gene            21689..22174
                     /locus_tag="O0V33_000328"
     CDS             21689..22174
                     /locus_tag="O0V33_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCX8975016.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRKRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFVGRPVHIMALTI
                     K"
     gene            complement(22343..23125)
                     /locus_tag="O0V33_000329"
     CDS             complement(22343..23125)
                     /locus_tag="O0V33_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCX8975017.1"
                     /translation="MNAVFILALLLLFLMIIFGGKKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMIQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(23122..24240)
                     /locus_tag="O0V33_000330"
     CDS             complement(23122..24240)
                     /locus_tag="O0V33_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCX8975018.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMTDGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            complement(24355..25239)
                     /locus_tag="O0V33_000331"
     CDS             complement(24355..25239)
                     /locus_tag="O0V33_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCX8975019.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYNESDV
                     SHMLLPLIQTFHLQNDTHVIPNLLEHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLGPFAESVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSCIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
     gene            25421..29920
                     /gene="gltB"
                     /locus_tag="O0V33_000332"
     CDS             25421..29920
                     /gene="gltB"
                     /locus_tag="O0V33_000332"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCX8975020.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILG
                     SEHEVVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKH
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKFYTDLSDDLYQSKLGL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHLAAI
                     DQEHFKLTYLSTVYEGNLEDALEVLGREAVDAVKQGAQILVLDDSGLVDGNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGTQSKLSGISIDQIDAENKERQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRVEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATGGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
     gene            29938..31401
                     /locus_tag="O0V33_000333"
     CDS             29938..31401
                     /locus_tag="O0V33_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HCX8975021.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDFVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLTAAEELNLLGYQVTIYERARESGGLLMYGIPNMKLDKDVVRRRIKLMEEAGIT
                     FVNGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
     gene            31674..31766
                     /locus_tag="O0V33_000334"
     tRNA            31674..31766
                     /locus_tag="O0V33_000334"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31710..31712,aa:Ser,seq:tga)
     gene            32219..33646
                     /gene="treP"
                     /locus_tag="O0V33_000335"
     CDS             32219..33646
                     /gene="treP"
                     /locus_tag="O0V33_000335"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCX8975022.1"
                     /translation="MAVKREDVKAIVTAIGGKDNLEAATHCVTRLRLVLKDESKVDKE
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKKAAAQKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKELIVMYPQIADISNMIN
                     VIANTAFIFLPALIGWSSMRVFGGSPILGLVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLVVTGIA
                     IVVPCILTIVMSNFSKQKAKEIVED"
     gene            33710..35350
                     /gene="treC"
                     /locus_tag="O0V33_000336"
     CDS             33710..35350
                     /gene="treC"
                     /locus_tag="O0V33_000336"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCX8975023.1"
                     /translation="MLKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFERLINVAHEKGIKVMLDIVI
                     NHTSTEHEWFKAARKSKDNPYRDYYFFKASEDGPPTNWHSKFGGNAWKYDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTVGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKDSIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLACEGRVVIQTGTVENNTISGFGAIVIETNA"
     gene            35375..36103
                     /gene="treR"
                     /locus_tag="O0V33_000337"
     CDS             35375..36103
                     /gene="treR"
                     /locus_tag="O0V33_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCX8975024.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRTK"
     gene            36165..36434
                     /gene="ffs"
                     /locus_tag="O0V33_000338"
     ncRNA           36165..36434
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="O0V33_000338"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            36504..36605
                     /locus_tag="O0V33_000339"
     CDS             36504..36605
                     /locus_tag="O0V33_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975025.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYMKFDSSGMDAQFE"
     gene            36744..37268
                     /locus_tag="O0V33_000340"
     CDS             36744..37268
                     /locus_tag="O0V33_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCX8975026.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEMNSDDKTYYGLAIASLSVNPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            37337..39034
                     /gene="dnaX"
                     /locus_tag="O0V33_000341"
     CDS             37337..39034
                     /gene="dnaX"
                     /locus_tag="O0V33_000341"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCX8975027.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPTQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            39124..39441
                     /locus_tag="O0V33_000342"
     CDS             39124..39441
                     /locus_tag="O0V33_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCX8975028.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            39448..40044
                     /gene="recR"
                     /locus_tag="O0V33_000343"
     CDS             39448..40044
                     /gene="recR"
                     /locus_tag="O0V33_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCX8975029.1"
                     /translation="MHYPEPISKLINSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKNDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            40386..40461
                     /locus_tag="O0V33_000344"
                     /pseudo
     CDS             40386..40461
                     /locus_tag="O0V33_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        13737 a         5903 c         8419 g        12861 t
ORIGIN      
        1 taagtctaat aaaaatccta agttattgtt caaaggagca ggtaatataa aagggtcttc
       61 tgttggatat aaggagatac agataatttt caataggaac aaagaagaaa gtgtttcttg
      121 tattgatagt atagagttta aaccgagtga aggagactat aatgagtaag agtgtctata
      181 aattagatgt tggcaaatta aaaaagatac tgaaccgaca agcgcaatat catttatggc
      241 gtatgaaatt gaaaatgcta ataatcataa tttgagtaag atggcaataa aggaacagtt
      301 agatagaagc gcagaaaggg gcatgttccc ttctaatctt gaatatgttg atagttacac
      361 tgattctctt actggagtaa caacttctgc ttttttaaat aaagatacag gcaaagtaac
      421 tcttgggatg actgggacta atatacaagg cgaagccttt aaaaagttaa aagaaggtga
      481 attttcaaga caaaatgtta ccaatgcttt ggaaacagtt aaagatggat atgcagatat
      541 taaaatatta tattctcctg catctgatca aaactataga tatgcgaata cacaagaatt
      601 tataaataaa ataaaaagta agtatgacat tgattttatt actggacatt cactaggtgg
      661 aagagatgcg gtagttctag gaatgagtaa tggtattccg aacattgtgg tttataatcc
      721 agctcctatt tctataacta gtttgaatcc taattcccca gatggaaaac gattgttaga
      781 attatataaa aattataaag gtaatattac taggtttgtt gcagaaaatg atgcattgac
      841 agaaaatctg aagaaatata agcattatgt ttttttcggt aatgataaag tctttaaaaa
      901 tggtaaaggt catgaaatgg aaggctttct gaccgaagaa gaacaaaaag ctataaaaaa
      961 agaacttaaa aaactaaaag gttatgcaga agaaaataat aagtcatttg taaagcattc
     1021 aaataatgct atctctaaat tagctagtat agaattactt agagccaata tgatgactgc
     1081 aaatggagga ggattatctt cttcgcagca gaaagtttta gacagtttaa cagctttaac
     1141 aattgcgcag tcattcagtc aactgataga cgatgaaatt aatcaaatca aaaaaatgta
     1201 taatgaaaag aaaaagaaat ttggaaaaaa ttgggaagac gctcaaaaag ctggaaaagc
     1261 tgtaggtgaa gatttgagtg taaatgaagt tcttaatgct ttagatgaag gtcaagtgaa
     1321 tgaaagtagt atggtaagag aacctgaaca aatgatatct gcaaaagaaa gacaactttc
     1381 aacgataggg tcttctgtat caaattatat tatgagagtt agactcagta ttaatgaaat
     1441 cgtagataaa gatcaagtgc ttgcatcaca aataggtgga ttactatgat gtttaatcaa
     1501 attaataata aaaatgaatt agaagaatca tatgaatccg agaaaaaacg tatagagaat
     1561 gaactgcaaa atttaaatga acttaggcat agaactcgaa aagaaaatga acgtagttat
     1621 gatgtttttc aatatttgaa gcacgaaatg aattatagtg aagatgccca aaggaaaatg
     1681 acgagaaata tagaagcgta tgagcaagaa atcaatgaga taattagaaa gcaagaatgg
     1741 aaattagaag aatataaaga agacttaaaa aagtcttatg aaaagcagtt agataaacta
     1801 agtgactgat aatttggagg agattaaatg actctaatag aaccagatat gaccttaaga
     1861 atgccagata taagtactac agtagaaaca cttaatctca tatctaaaat gaatgctcaa
     1921 aaagaaaata ttcgcacagt tattgcacct gaacataagc ataaatacaa agatattgaa
     1981 aacggattaa aaggtgaaga aaaagtatta attgaacaaa tggcgcaaca ttgcgaggct
     2041 tttaaagcta attttaaagg cgcagctcaa ggagattggg ttaaaagtgc catgtctgag
     2101 atagacagca ttaaggatga cctgaaaaaa attaatagct aaactacata tcattatact
     2161 aaataaagcg accaatgttc agtgtattca caactgacac taggccgctt ctttttaatt
     2221 tattttattt ccatgtaaag taacgctctc ttaatcgaaa tatcacactc acaattgtaa
     2281 aaacacaact taaattactg tcccgcatta aacatatcca cattccaaac agcaacaaca
     2341 aaatcctcaa gtaccaaaag ctatgaccac tttatcaaaa tcaacgcaag ttaacctctc
     2401 ctgaactacc cgccataaat catcttcaac ttacaaacca taaagcgctg cagttaagta
     2461 ttgaatggca tagccaattg aaataaaaat agcaatcgtt ataatagcac tgccaatggt
     2521 atataaagta tgtttcgtag ataatccttc agtctttgaa ccaaaaacac tgagtagaat
     2581 aaatgcaata ccaatgatat ataaaactaa aatgacaaat aacatgttta tacacctcga
     2641 ttctctctcg tgagatatat ctttgtctct attataaagt aaagtgctca ataatttaat
     2701 taagtatact atttttttat tatcgaagat tgcgaaaggt gaaatgctcc tatataatgt
     2761 aaaacggaat tattcctatt tacaaataaa ttgaagtgag ggtatatata tggctaaaat
     2821 tccagttacg gtattaagtg gttatttagg ctcggggaaa acaacgttgt taaatcatat
     2881 tttacaaaat cgtgaaggtc gacgtatcgc agtaattgta aatgatatga gtgaagttaa
     2941 tatcgataaa gatcttgtcg cagatggtgg gggactatcg cgcacagacg aaaaattagt
     3001 cgaactttct aacggttgta tctgttgtac acttagagac gatttattaa aagaagttga
     3061 gcgtttagtg aaaaaaggtg gcatcgatca aattgttatt gagtcaacag ggatttcaga
     3121 gccagtacct gttgcacaaa ctttctcata tattgatgat gaacttggca ttgatcttac
     3181 agcgatttgc cgtttagata caatggttac agttgtggat gctaaccgct tcgtccatga
     3241 catcaactca gaagatttat tgatggatcg tgatcaaagc gttgatgaaa cagatgagcg
     3301 ttcgattgct gatttattaa ttgaccaagt tgaattttgt gatgtattga ttattaataa
     3361 aattgactta ataagtgaag aagaactagc gaagttagaa aaagtgttaa gtgcattgca
     3421 accgactgct aaaattatta agacaacaaa ttctgaagta gatttaaaag aagtcttgaa
     3481 tacgcagcgt tttgattttg aaaaagcgag cgagtcagca ggatggatca aagaacttga
     3541 gtctggtggg catgcatcgc atacacctga aacagaagaa tatggtatat catcgtttgt
     3601 atataaacgt cgtctacctt tccatgctaa aaggttcaat gattggttag aaagcatgcc
     3661 aaataatgtc gttcgatcaa aaggtatcgt atggttagca caatacaatc atgtagcatg
     3721 tttattatct caagcagggt catcttgcaa tattcatcca gttacatatt gggtggctag
     3781 tatgtctgaa gcgcaacaaa cgcaaatatt agcagaacgc caagacgtcg cagctgaatg
     3841 ggatccagaa tatggcgatc gtcatacaca atttgtcatt attggtacag acttagatga
     3901 aggtgcaatt acaaaagaac tcgatgcatg cttagtcaat gcgcaagaaa ttgatgcaga
     3961 ttggcaacaa tttgaagatc catatcaatg gcaaattaga ccggcacgat aagttgaatg
     4021 aagtgtaatc atttttaaat tgtggcttaa ttgtttgcaa tttatgacaa ttaataaaaa
     4081 gttaagatgt tcttaggatc tttttgtttt caatctatct aaagtgtgat agttttgata
     4141 aagcagaaat ttgcatttta tccatagggc taggacatgt atgtgtctta gtccttttta
     4201 tatttgcgtt gatgaaaaat gacaaaacca tcgtaccaac tgttaattga aagtacaatc
     4261 attgtttaaa ggtcataaaa atgagtgaag caaatgcagc tatcaatgta ctattactgc
     4321 ttacagtcac acgagtgttt cagcctaaac gtgcaatgaa attctgcacg cgtccaatat
     4381 atcatgcgct agatcatgaa gatcattaag atttcacatg ccaaatcatg aatgtctcag
     4441 acctcgagta tcaaacataa aacacgccac aaaaatataa cgcgtcagac tacaaagatc
     4501 acgaatattc cgtacgtccc actaagaaca tcctatacaa aaaacaccaa gaccatttat
     4561 ttgcaacaaa ctacccagac tcatctcaca tcaataaatc aacacaaagc aaagccacca
     4621 tccctattgg tatagtggcc tgagaatttt aagtattcaa ttcgcttaaa ttattttgcg
     4681 aaaatgtcga taattgcttt gatgatttta atgatagtac ctacaatagc catcgttttg
     4741 tcctcctgta tgttgattta acatagtgat gagttgttga tcgttaatgt ttgagattag
     4801 tagttaccta aaaatttacc aagtaaatct ttaaagaatt tgaataattt tgctacgaat
     4861 tccatgtgaa tggccccctt caaataagat gttcatatct atgatatttt tataacttac
     4921 ttaccagtga atttctcaat taatccttta atgaatttaa tgattcctgc aatgataccc
     4981 atgtgaaaga cctcctttgt ttgttatgaa aacttattta ccagtgaatt gttcgattaa
     5041 gcttttgata actttaatga tgccagcgat gatacccatt aagattacct cctttgctta
     5101 tgagttaact tcattgtaca tagttatctt gtgcgtaatt gatttttttc ctaacttgaa
     5161 ttgttatgat cataactgcg aattttttat tttaaaagga cgggaaatac tgcctaactg
     5221 tacaggtgca tttacaaaat ctttacaatc attagtttag tggggttaag gtcatgaatt
     5281 tgacatgaag cagaaattta attatatttt atgttataag tttaaaacga ataacacgtt
     5341 aggtctcgtc taggcaaagc atatttatga agatgaattg aacagacaga agtaacattt
     5401 tatatacata tgtgcatggg agcacgctta aagagaacag tgtaacaagg tatatcaaca
     5461 taccatggtg cagtgtctag ttaaactgaa acctgcaata gtaaattttc tgccattatg
     5521 tacagaatct actattgtag gtttttcttt ttatacataa gcattatgcg agagattaga
     5581 aataaggagg ttatgcagtg ttaagttttc aattgctatt ttcactgttt gttattgcgc
     5641 ttatcattgc attgataagt ggcttgttgt ttttagcacc agttatgcca atgagatata
     5701 ttaaattaca tttatacata ctagtcatgc cagtattatt tgcagtcatt ggctttttcg
     5761 gtattcatgg tcaacatgtc ttaggtccat ttaaaataga tcgtttatct tggttattag
     5821 ctggctttgt aatggcgctt ggctttatta ttcaaaagtt ttcaatgcga tatttactag
     5881 gtgatcatca ttatagacat tactttccat tgttcactgc gattacatcg tttgcatctt
     5941 tagcatggat gtctgaagac ttaagactga tggcactctg ctggggtatg acattattat
     6001 gtttaacatt gctgatgaac gttaatcgtt tttggaaagt gccacgtgag tctgcgaaat
     6061 tatcaagcat gacattttta tgtggttggc ttgcattcgt tggagcaatt gtatctattt
     6121 atattgcaac tggcgagtgg cgggtaccac aacatatagc gcatccgaca tggtcattgt
     6181 tgacgaatgt actacttgta ttagctgtca tgataccggc agcacaattt ccttttcatc
     6241 gatggttgat tgaatctgta acagcaccaa cgccagtatc ggcaattatg catgcaggaa
     6301 ttgtgaatgc aggtggtgtt attctaactc gttttgcgcc gatatttgat aatggatttg
     6361 cgttatcatt attacttatc ctttcaagca tttctgtatt gttaggatcg ggtattagct
     6421 tggttcaagt tgactataaa cgccaattgg tgggctctac gatgagtcaa atgggcttta
     6481 tgttagttca atgcgcactt ggtgtatatt cagcagcgat tatacattta atattgcacg
     6541 gtatttttaa agcaacatta tttttacaat caggttctat cgtgaagcga ttcaatattc
     6601 caaaacaagc atctgctaaa gacgcttatg gctggattgt catgggacgt gtattagcta
     6661 ttatcgtggc atttctgttt tggatgagta gtgacagaag tgcatatgaa gtgttaagtg
     6721 cactcattct agcatggtca ttacttgtat cttggaatca aatggtagcc tttagtaaag
     6781 ggcgtatggc acgtctggtt ggcatgattt tgatttcaat tgtgacattt atctacatca
     6841 tcacacataa ttatttttac gatgtattac aaaatataac aacacatgcg acaacgccgc
     6901 ctacaattag tgtcatcatt agtgttgtca tattaatatt tggtagttta ttaagtatct
     6961 gggtagcgcg tcatcgatac tctaagactt ttgcggtatt gtacgtgtgg ttagttaatc
     7021 taggagaagc acgctcgaaa gcgatagaaa gtcatccgaa ttatttgaag aagtatttat
     7081 aggaggtgaa aggtatgaca acacagttaa atatcaattc agtcattgaa aatgcgaaac
     7141 gtgttattac accattatca ccgatttcga tttttgcagc acgaaatcca tgggaaggat
     7201 tagaagcgga tacgtttgaa gatgtcgcaa aatggttacg tgatgttcgc gatgtggata
     7261 ttttcccaaa taaagcatta atagaaagtg ctgtggcacg cggtgaatta gacgaaagtg
     7321 tctttaatca acttgttact gatatgctac ttgaacatca ctacaatatc ccgcaacact
     7381 acatcaatct ttatattgat aacattaaaa cattaaaaga cgtacctgca tcatacatga
     7441 atcattcaaa tgttgatgtt gttgctgatc tactattaga aaaatcaaaa cgtgatatgg
     7501 ctgaatcata tcatcactat gatgtacgtc cgatgagtga tgcaataata gatgaacaag
     7561 gtgagccact tagcgaacaa gtgaatcgtc aaatgattaa atggacgaaa ctttatatcg
     7621 atcaatttct atcgagttgg acaatgccga agcgtgagca aagtttttac catgcatggt
     7681 tgcatttagc gcaacatgac catagtttta ctaaagcaca gcgccaagtg attaaaggct
     7741 tacccaatga tcctaaaatg acgatagagt cagtattaaa tcatttttca atagcgcagg
     7801 aagactacca agcttatgtt gaaggacatc ttttggcgtt accgggttgg gcaggtatat
     7861 tatactaccg ctcacaacag catcactttg aacaacattt gttaacggat tatttggcca
     7921 ttcggttagt tgtcgaacaa ttgctagttg gtaatgagtt taagtcagtc actaaagatt
     7981 gtgaaagcag atcggaaaat tggtttaagc aaactgttgc atcatggtgt tactacagtg
     8041 atatgcctag cgatgtatta ctacaacatg acgtcaatga aattcaaacg tttattcatt
     8101 ttgcagcaac tatgaataaa aatgtattta aaaatttatg gttgattgcc tgggaaatga
     8161 catacgaatc tcagttaaaa caaaaaatta aagcaggtca tgaaagtgtg gcgggcgcat
     8221 tagatgtaaa ccaagtaaat gttactgaaa atgacaacgc taatcagtca cattcagtat
     8281 cgttaaatgc cacacaagca gttgatgaaa ataatagcga gctaaatcag gtgggcacat
     8341 caacgaaagc gcaaattgca ttttgtatag atgttcgttc agaaccattt cgtagacata
     8401 tcgaagcagc agggcccttt gaaacgattg gtattgcagg tttttttgga ttacctattc
     8461 aaaaagatgc cgtagacgaa caattcaaac atgattcatt acctgtcatg gtaccgccgg
     8521 catatcgcat taaagaattt gcagaccgct acgatatgaa tgtttatcga caacagcaac
     8581 agacaatgtc atcgatgttt tacacattta aattgatgaa aaataatgtt atgcctagtc
     8641 tgttattgcc tgaattaagt gggccatttt taagcttgag taccattgtc aattcgatta
     8701 tgcctagaaa aagtcgcgtg tctttacaaa aaataaaaca aaaatggtta aaaaagcctg
     8761 aaacaaagtt aacgattgat cgtgagtttg acagaacatc agacttacct gttggattta
     8821 ctgaacaaga gcaaattgat ttcgcgttac aagcgttgaa attgatggat ttaaccgaag
     8881 catttgcgcc gttcgttgtg ttagcaggtc atgctagtca ttctcacaat aatccacatc
     8941 acgcatcact tgaatgtggg gcttgtggtg gtgcatcaag cggttttaat gctaagttat
     9001 tagcgatgat atgtaatcgt ccaaatgtca gacaaggatt aaaacaagca ggcgtgtata
     9061 ttccagagac tactgttttt gcggcagcag aacatcatac gtctactgat acattggcat
     9121 gggtatatgt gccagacaca ttatcagctt tagctctaga tgcatatgaa tcattgaatg
     9181 acgtcatgcc gatgatttct gaacacgcga atcgcgaacg tttggacaaa ctgccaacga
     9241 ttggtcgtgt gaatcatcca gtggaagaag cgcagcggtt tgcaagtgat tggagtgagg
     9301 tacgtccaga atggggcttg gctaaaaatg catcatttat aattggacga cgccaattaa
     9361 caaaaggcat tgatttagaa gggcggacat ttttacacaa ttatgattgg cgtaaagata
     9421 aagatggcaa attgttaaat accatcattt ctggtccggc gcttgtggca caatggatta
     9481 atttacaata ttatgcgtcg acagttgcgc cgcattttta cggaagtggg aataaagcga
     9541 cacaaaccgt cacgtcaggt gttggtgtca tgcaaggtaa tgcgagtgat ctgatgtatg
     9601 gcttatcatg gcaatctgtt atggctgctg atcgaacgat gtatcattcg ccaattcgtt
     9661 tgcttgtcgt tgttcaggca cccgattttg ttgtagctag actactcgcg aataatgagc
     9721 atttcgctag gaaggtgtct aatcattggc tgcgtttaat gagcgttaat gaggaagggc
     9781 gttttaaaag ttggatttaa cgatgaaatg ttgcgatgct aagtaactaa tatatcatca
     9841 tatctacaat gttctagctt cttaaaatat actaaaaatg ataaacttaa tgttatagtt
     9901 gatgtcgtga taacgataca aagatgaagt aatcaatttt aatattattg gagttagtga
     9961 tatgaaaaga acgaaaggtg aaatcgaagc tgaaatcagt aaagccatta cgcaatggga
    10021 aaaagatttc cttggcagag gttccttgtc ggttaaatca gacattttaa gagatatggt
    10081 gattattagt ttacaaggta tcttaacgcc agcagaatat cgtgtgtgta gtacgaatga
    10141 aggattatta aatattaaac gaacacgttc tgaattagtt gaatccggtg agcaagattt
    10201 gaatgatatc atttttaaaa ttacaggtat caaagtgatg agcttccata gtgatttaag
    10261 tacagttaca ggtgaacgta ttatcgtatt caaacttgag gataatttgg aaaagcatat
    10321 ttaagaaggt taggtatgct tctgcaggct tattaaagct atagaagtat gcttggttaa
    10381 acagcgcaac gccatatatt tgggtgtgct gtttttctat tatatgatgt gtttatcatg
    10441 tgtatgtatg cattggtttg aatatagccc gtgaaaggtg tatgtcataa aatttgtgaa
    10501 tggtgacaag tcaacgcaag catatttgtt gcgagacatg cccactatcc ttttacataa
    10561 agagtatatg tgtgacgtag gcatataatc gataaagtat acctaaaaaa ttaggtataa
    10621 tgaccattgt tgcaataagt ttttcggcct tgaaaccgat aacgcatagt ataataggaa
    10681 taaaatataa tacaaatatc cagtaaatca aatttgaaaa cgatggatgt ataggggtaa
    10741 agaatctaac taatgtaatg attaacgcta tatagacaaa aattactcta ttgatacgcc
    10801 ctaccccctc tgtataataa atatagatcg tactaaattg aaaatagcaa aatattgtta
    10861 tttcaattat acaatgtttt atttgcaata tacataacaa tatgaacttt ataattttga
    10921 tattgtcggt aaagtgaaat ttgtggcaca ttgatattat ggataacatt tgtaaaatga
    10981 aaatgatgat acgaggagat tgtaagatga tagataaaaa attaacatca ccgaaaatga
    11041 cagtgccttt atttttaatc gcgctgattg tatttatagg tatgttttac agtgtagtga
    11101 caaatcaaga atggcttaaa aatatagata tgggatcatt aacatggttt acagattatt
    11161 tcggtgagcc acaacgccag tatgttaaca atttatttaa ttactatatg acgtttagtg
    11221 cggaaattgg agatgtcaaa ggtgtcgtgt tgatttccat tatcgtcaca atcatactgt
    11281 ttattaaaca gaggcattta gcggtttggt ttgtgacata tttggtttca ggtgtcatca
    11341 tgaacaaatt aattaaagat actgttttac gtccaagacc atataatcat ttagccgttg
    11401 atacaggctt ttcatttccg agtggacatt ctaacgccag cacattatta tatttcgcct
    11461 taatgatcat tattatttca gttgctgcta agacaatgac aaaagtgttg agtgcgctgg
    11521 ttatgggaat attatggctt agcatattat tttgtcgcct ttattttcat gcgcattact
    11581 tttcagatgt cattggcggc acgtcactag caatcatttg ggtagcgtta ttcttaatgg
    11641 tatacccata ctttattaat catcgacgac aacgcgttta gcaaacatct ttagacatag
    11701 tggaggtata tagaaaaatg agaattaaaa caccgagtcc atcttattta aaaggcacaa
    11761 atggacatgc gatattatta ttacattcat ttacaggtac aaatcgggat gtgaagcatc
    11821 ttgcagctga gttaaatgac caaggattta gttgttatgc accgaattat ccaggtcatg
    11881 gtttattatt gaaagatttc ataacatata atgtagatga ttggtgggaa gaagttgaga
    11941 aagcttacca atttttagtc aatgaaggtt atgaatctat cagtgcaacg ggtgtgtctt
    12001 taggtggatt aatgacatta aaattggcgc aacactatcc tttgaaacgt atcgctgtca
    12061 tgtcagcacc aaaggaaaag agtgatgatg gcttaataga acatttagtt tattatagtc
    12121 aacgcatgtc gaatatttta aatctagatc agcaagcatc gagtgcgcaa ttagcagcaa
    12181 ttgatgatta tgaaggtgaa attacgaagt ttcagcattt tattgatgat atcatgacaa
    12241 atttaaatgt tgttaaaatt ccagctaata tattatttgg tggtaaagat gcgccatcct
    12301 atgaaacaag tgcacacttt atttatgaac atttaggatc agtagacaaa gaattaaatg
    12361 gtctgaagga ttctcatcat ttaatgacgc atggagaagg cagagatatt ttagaagaaa
    12421 atattattcg ctttttcaat gctttaacat aattgaagat tttaaaaatt aacacttgag
    12481 tgacgatgtt attatatttg tcatttgagt gttttttgtg gcgtgatagt atagggaact
    12541 tagacaaagt taatttgtat tgtgatgtcg tggaaatatt atgtagttat acctgaagtt
    12601 gatttgtatt gtgatgttgt gggaattttg tgggcattgt acctgaagtt gttatgtggc
    12661 gagatgttgg ggatatggat ggtgttgtat gtggcgagat gttggcgcaa gtatacagcg
    12721 ttgaaccaga aattttaatg tagcgtaatg acaacacgtt acaagggatt tcctatatta
    12781 tgataaactc aggcgctacc gtattaatga aacaattttg atgcaggtgt attgcatgtt
    12841 atttacatac atgttagagt gttacttaaa cacgtagacg ctttaacgct ttaaaggaga
    12901 tgtgaatgat gaaaagacaa caatcacaat ggaagtcatc aactggattt attttagcta
    12961 gtgcgggttc tgcaatcggt cttggtgcca tgtggaaatt cccatatatg gcagggatct
    13021 atggcggcgg tgcctttcta gctatgttct taatattcac catttttgtt ggattaccat
    13081 tactcattat ggaattcact gttgggaaaa tgggacggac atatacaaca caaatatata
    13141 gtaaattaac tggtaaaaaa tggctcaata tcattggctg gaacggtaat ttggcagtgt
    13201 ttattttatt tggcttctat agtgttattg gtggttggat tgtcatttac atcggacaag
    13261 tattatggca attagttata tttcaacgca tcaatcatct ccaagaaatg aattttgaag
    13321 cggtaatatc aaatccttgg ttaaccgttc tagggcaagg tatattcata ttcgctacga
    13381 tgattattgt catgttaggt gttgaaaagg gattagaaaa ggcatcgaaa gttatgatgc
    13441 cgttgctgtt tgtcttttta atcatcattg tgattaagtc tttaacatta gatggtgcct
    13501 tagaaggtgt gaaatttatt ttacaaccaa gagtgtcaga gattactgct gatggaatct
    13561 tgtttgcgct aggtcaatca ttctttacgt tatcattagg aactacaggt atgattactt
    13621 atgcgagtta tgcctctaaa gacatgacga ttaagtcatc agctatttct atcgttgtta
    13681 tgaatatctt tgtatctgta ttggcaggcc tagctatatt tccggcgtta catagttttg
    13741 gctatgagcc acaagaaggg cctggattat tatttaaagt actgccaatg gtctttagtc
    13801 aaatgcatct aggcacatta ttctatttgg gattcttagt gctgttctta tttgcggctt
    13861 taacgtcatc tatttcttta ttagaattaa atgtttctaa cttcacgaag aatgacaata
    13921 caaaacgtaa aaaagtcgca gtgatcggta gtatattagt atttatcatt agtattccag
    13981 caaccttatc ttttggtatc ttaaaagatg taagattcgg tgcgggaacg atttttgata
    14041 atatggattt catcgtttcg aatgtattga tgccattagg cgcattaggt actacgcttg
    14101 tcgtaggaca attattagat aaaaagttat tacaacaata ttttggtaaa gatcgattta
    14161 gattattcag tggttggtat tacttaatta agtatgcgat gcctgtcgtt attattttag
    14221 tctttatcgt gcaattattt agttaatata tagaagcatc tgacacacaa gtatttgtgt
    14281 tgctcaggtg ttttttgttt ggaattgaaa attttgtact ggttctgaat taatcgaaac
    14341 ttagataatt gcaaaataac aaaatgtcgt ttataattct tatcttaaaa gtaagaatta
    14401 aaattgagac atgaagaaag gtttcattca tttgatcggc atgtcagatg agggtgcatc
    14461 tatgattact tatgatttaa ttggcaatac accattagta ctgttagaac attatagtga
    14521 tgataaagtt aaaatttatg ccaagcttga acaatggaat cctggaggca gtgttaaaga
    14581 cagactcggg aaatatttag tagagatggc aattcaagaa gggcgtgtgc gtgcaggtca
    14641 aattattgtt gaagcgactg ctggcaatac aggcataggg ttagctattg cagcaaatag
    14701 acatcatttg aaatgtaaga tctttgcgcc gtatggtttt tcagaagaaa agattaatat
    14761 tatgatagcg cttggtgcag atgtttcaag aacgagtcag tctgaaggta tgcatggggc
    14821 acaattagct gcacgttcat atgctgaaaa atatggtgcc gtttatatga atcaatttga
    14881 atccgaacat aatccggata catattttca tacattggga cccgaattga cttcagcatt
    14941 acagcatatt gattattttg tggctggtat tggctctggc ggtacatata caggtaccgc
    15001 acgttattta aagcaacatc acgtgcaatg ttatgccgtt gagccagaag ggtccgtgtt
    15061 aaatggaggg ccagctcatg cacatgacac tgaaggtatc ggttctgaga aatggccgat
    15121 atttttagag agaagtcttg tagatgggat atttacgatt aaagatcaag atgcctttcg
    15181 aaatgtcaaa agtttggcaa agcatgaagg attattggta ggcagttctt caggtgcagc
    15241 attacaaggt gcattgaatt taaaagcgca attatctgaa ggtacgattg ttgtcgtatt
    15301 tccagatggt agcgatcgat atatgtctaa ggaaatattt aattatgagg agaatgatta
    15361 tgaataagaa aacgaaatta attcacggtg gacacacaac ggacgactat acaggtgcag
    15421 ttacaacacc aatttatcaa acaagtacat atttacaaga tgatattggt gatttacgtc
    15481 aaggatatga atattctcgt actgcgaatc caacaagaag ttctgtagaa agcgttattg
    15541 cgtcattaga aaatggcaaa catggctttg catttagttc aggtgttgca gcaatcagtg
    15601 cagttgttat gctgttggac aaaggtgatc atattatttt aaattcagat gtatacggcg
    15661 gtacttatcg cgcattgaca aaagtattta cacgatttgg cgttgaagtg gattttgtag
    15721 atacaacgca tacagattca attgtacaag cgatacgccc aacaacaaaa atgttattta
    15781 ttgaaacacc ttctaatcca ttattacgtg ttactgacat taaaaagtct gctgaaattg
    15841 cgaaagaaca cggtttgatt tcagtcgttg ataacacatt tatgacactt tattatcaga
    15901 atccattaga tttaggcatc gatattgtct tacattctgc aacgaaatat ttaggtggac
    15961 atagtgatgt cgttgctggt ttagttgcaa catcggatga caagcttgca gaacgtttag
    16021 catttatttc aaattcaaca ggtggcattt taggacctca agatagttat ttacttgtga
    16081 gggggattaa aacattaggt ttacgtatgg aacaaattaa tcgcagtgtt attgaaatta
    16141 ttaaaatgtt acaagcacat ccagctgtgc aacaagtgtt ccatccaagt attgaaagtc
    16201 atttaaatca tgatgtccat atggctcaag cggatggcca tacaggtgtg attgcttttg
    16261 aagtgaaaaa tacagaaagt gccaaacaat tgattaaagc aacatcgtat tacacattag
    16321 ctgaaagttt aggtgcagtg gaaagtttaa tttcagtacc tgcattgatg acacatgcat
    16381 ctattccagc agatattcga gctaaagaag gtattacaga cggacttgta agaatttctg
    16441 taggtattga agatactgaa gatttagtag atgatttaaa acaagcacta gatacgttat
    16501 aaataatagc agcactggca tatattttga gttcaacgtt tgttgttctt gaatatgcta
    16561 gtgctttttt gtgtggtgtt aatatttcgt aattgcattg tggaggattg ctggtcattt
    16621 ttattcattt aatttgataa atgtcatagt agtctcacaa ttcgtcattg tcacaaggta
    16681 ttatttttta attttttaga aaattcggca aattgtattg acgatatatg caattttaca
    16741 tataatgctc ttattcctag tggattaata agaatttgta ggaggggcga tgatgattga
    16801 gtttcgacaa gttagtaaat catttcataa gaaaaagcaa acaatagatg ctttgaagga
    16861 cgtatcattt acggtcaatc gcaatgatat ttttggtgtg attggatata gtggtgcagg
    16921 aaaaagtacg ttggtaagac tcgtgaatca tcttgaagct gcctcgaatg gacaagtgat
    16981 tgtagatgga catgatatta cgaattatag cgataaaatg atgagggata ttaagaaaga
    17041 tatcggtatg atatttcagc attttaattt actaaattca gctacggtat ttaaaaatgt
    17101 agctatgccg ctcattttaa gtaagaaaag caaaacagaa attaagcaac gagtaacgga
    17161 aatgcttgaa tttgtaggat tgagtgataa aaaagaccaa tttcctgatg aattatctgg
    17221 tggtcagaag caaagggtgg ctattgcaag agcgcttgtt actaatccga aaatactcct
    17281 atgcgatgaa gcaacaagcg cattggatcc agcaacgact gcttcgatat tgacgttatt
    17341 aaagaatgtc aatcaaacct ttggcattac aattatgatg attacacatg aaatgcgcgt
    17401 tattaaagac atttgtaatc gtgttgctgt aatggaaaag gggcaagtgg ttgaaacagg
    17461 aactgttaaa gaggtgttta gtcatcctaa aacgacgatt gctcaaaatt ttgtgtctac
    17521 agttatacag actgagccaa gtccatcatt gattcgtcga ttgaatgaca aacaagttgg
    17581 cgattttaaa gattataaaa tcttcgtcga ggaaactcag atgacacaac cgattataaa
    17641 tgacttgatt caaatttgtg gcagagaggt taaaatttta ttttcatcca tgtcagaaat
    17701 acaaggtaac accgtatgtt atatgtggct tcgatttaat atggatcaac aatttgatgg
    17761 cacggcaata aatcaatatt tcaaagagaa aaatattcaa tttgaggagg tgcattaaca
    17821 tatgtttggt tctgatttag acagtgcaca gttattacaa gcattatacg aaacactata
    17881 tatggtatct attgctttat ttttaggagc agtgattggt attccattag gtgtcttatt
    17941 ggtaattact cgaaaacaag gcatatggcc caatatagtg atacatcaag ttttaaatcc
    18001 tataatcaat attttaaggt cactaccatt tattattttg ttgattgcga ttgtgccatt
    18061 cacaaaatta gtagtaggta cttccattgg tacgactgct gccatcgtgc ctttaacagt
    18121 atatgtggca ccttacattg caagacttgt tgaaaactca ttattggaag tagacgaggg
    18181 gattattgaa gcggcgaaag cgatgggcgc ttcaccacta cagatcatta gatatttttt
    18241 aattcctgaa gcgttaggtt cattagtatt agcaattacc actgcgatta ttggacttat
    18301 tggaagtacg gcgatggcag gcgctgttgg cggtggtggt ataggagact tagctttagt
    18361 gtatggttat caaagatttg atacgacggt cattattatt accgttattg tattagtcgt
    18421 tattgtccaa gtgattcaaa cgctagggaa tgtcctagct agattcatac gtagacattg
    18481 atgatatata gtgaagattt tgaaaggaat tgatagaatg aaaagattga ttgggttagt
    18541 tatcgtagca cttgtattat tagcagcgtg tggtagtaac aatgataaaa aagtaacaat
    18601 tggtgtcgcg tcaaatgaca ctaaggcttg ggagaaggtt aaagaattag ctaaaaaaga
    18661 tgatattgat gtggagatta agcacttctc cgattacaat ttaccgaata aagcattaaa
    18721 cgatggtgat attgatatga atgcattcca acattttgca tttttagatc aatataaaaa
    18781 ggcgcataaa ggaacaaaga tttcagcatt aagtacaaca gttttggcac cgttgggcgt
    18841 ttactcagat aaaattaaag atgtcaaaaa ggttaaagat ggtgctaaag ttgtcattcc
    18901 aaatgatgtg tcaaaccaag cacgtgcact taaactatta gaagcagctg gtttaataaa
    18961 actgaaaaaa gatttcggat tagcaggtac agtgaaagat ataacgtcaa atccaaaaca
    19021 tttaaaaatt actgcagtag atgcacaaca aactgcacgt gctttatctg atgtcgatat
    19081 tgcagttatt aataacggtg tagcaactaa agctgggaaa gatccaaaaa atgatccgat
    19141 tttcttggaa aaatcagatt cagatgctgt aaagccatat attaatattg ttgcagttaa
    19201 cgacaaagac ttggataaca aaacatatgc taaaatcgta gaattgtatc attcaaaaga
    19261 agctcaaaaa gcgttgcagg aagatgtcaa agatggagag aaacctgtta atttatctaa
    19321 agatgagatt aaggcaatag aaacgtcatt agcaaaataa attatattac gtcctacaag
    19381 catagttcat gcttaagttt gtagggcgtt tttgtttgcc tataaaaatg tttcaaaaga
    19441 cagaaaggga ttgtaatcat tttatagtga aatattatga aattgtaata atttagatat
    19501 tgtaaaatct aataagttgt aataatttta agaggtaatt ataaaatttg atgacacagt
    19561 atatgatttt tttgtaatca taatgtcatc aaacatcaac ctattataca taattaaatc
    19621 gtataatgat gtagtattca taaattcgga taaaaagaat gttaggaaag ttatgcaaga
    19681 ggaggatttt aaagtgcaaa aaaaagtaat tgcagctatt attgggacaa gcgcgattag
    19741 cgctgttgcg gcaactcaag caaatgcggc tacaactcac acagtaaaac cgggtgaatc
    19801 agtgtgggca atttcaaata agtatgggat ttcgattgct aaattaaagt cattaaacaa
    19861 tttaacatct aatctaattt tcccaaatca agtattaaaa gtatctggct caagtaattc
    19921 tacgagcaat agtagccgtc catcaacaaa ttcaggtggc ggatcatact acacagtaca
    19981 agcaggcgac tcattatcat taattgcatc taaatatggt acaacttacc aaaacattat
    20041 gcgacttaat ggtttaaata atttctttat ttatccaggt caaaaattaa aagtatcagg
    20101 tactgcaagc tcaagtaatt ctacgagcaa tagtagccgt ccatcaacga attcaagcgg
    20161 tggatcatat tacacagtac aagcaggtga ctcattgtca ttaatcgctt caaaatatgg
    20221 tacaacatat caaaacatta tgagacttaa tggcttaaat aatttcttta tttatccggg
    20281 tcaaaaattg aaagtaactg gtaatgcatc tacgaactca ggatctacta caacgacaaa
    20341 tagaggttac aatacaccag tattcaatca ccaaaactta tatacatggg gtcaatgtac
    20401 atatcatgta tttaatcgtc gtgctgaaat tggtaaaggt attagcactt attggtggaa
    20461 tgcaaataac tgggataacg cagcggctgc agatggttac acaattgaca atagacctac
    20521 tgtaggttca atcgctcaaa cagatgtagg ttattatggt catgttatgt ttgtagaacg
    20581 cgtaaataac gatggtagta ttttagtttc agaaatgaac tattcagctg caccaggtat
    20641 tttaacttac agaacggtac cagcttacca agtaaataat tatagatata ttcactaaag
    20701 tcttacgtat ataaatatat ataatgaatt cctattacat ttcacaagct gaaaagttgg
    20761 atttaatcct aaactttatg gcgtgactat caaagtcgtg attattgtaa taggaatttt
    20821 tgtataagaa aaaggattga tcgaatgaac gtcatgctct atgttcgacc aatccttttc
    20881 tgatttagta ttcatgcttt actttgcgaa tgcttgtaaa taatctagca ccgtttgtta
    20941 ttaaagtaac aactgccact gcttttttga acttgcgtgg tgacttaatt gaaatgtaaa
    21001 agtctaacac ggttcctaca gtgcttaaac ctaatgaaag atatactaat tttttatttt
    21061 tagcatgata tttatagcca ttgtagccgt cgactatgaa acctgcgaca tttagtaaac
    21121 ttgataaacg ttgtgatttg gaacgttttg ccataataat aattccccct agtatcttct
    21181 gtttatatgg aataattggg tgcgctgaat tgctaacgtt ttgcgctata actactcata
    21241 tatgataaca taattgtaca gtataatttg aaaaattgat ttcacaaagt tggggtgtca
    21301 aagatgatta aatgtgtctg tttagtagaa gaaacagctg ataaaatatt gcttgttcaa
    21361 gtaaggaatc gcgaaaagta ttatttccca ggtggtaaaa tagaagaagg ggaatcacca
    21421 gtacacgcgc tgttaagaga agtaaaagaa gaattaaatt taacattaac aatggatgaa
    21481 attgaatata tcgggacaat tgtaggtcct gcatatccac aacaggatat gttaactgag
    21541 ttaaatggat ttcgcgcatt aaccaaaatc gattgggaaa acgtaactat caataatgaa
    21601 attacggata tacgctggat tgataaagat aatgatgcgt tgattgcgcc tgctgtcaaa
    21661 gtttggattg aaacgtatga tggtaaacat gacaaataat gacaccatca tgttacgaca
    21721 ttatgtccca caagattatt cgatgttaga agcttttcaa ttaagtgaaa gtgatttgaa
    21781 gtttgttaaa acgccagagg aaaatattac agctgcaatg tctgataatg aaaggtatcc
    21841 catcgttgta atggatggca ggcaatgtgt ggcctttttt acattacatc gtggaaaagg
    21901 tgtcgcacca tttagcgata accaagatgc agtatttttc aggtcattta gtgttgatca
    21961 acgttatcgt aaaagaggaa taggtaaagt ggtaatggaa aaattggcgt catttatcac
    22021 ttcaacattt caggatatta atgagattgt gttaacggtt aatactgaca atccacatgc
    22081 catggcactt tatcgccaac aaggatatca atatatggga gatagtatgt tcgtcggaag
    22141 acctgttcat attatggcgc taactataaa ataaattaaa tttaaaagca tctttactca
    22201 tcgtcgacca caacaattaa tgatgaataa aggtgctttt tgttatagac caccggacaa
    22261 tttactatag taaaaagcga cctagggaac aattgacata tatccacagg tcgcttacct
    22321 taagttatat tgctagttgc gattaattga tagactcatc atttttgcgc tgtcgagatg
    22381 gtctttttat taaaaatgcc gtaatccaag ccgtaatcgg aatactgatt gcaacggcaa
    22441 taccgcctaa aataatagaa ataaattctt gggcaaatat tttcgagttt ataatatgac
    22501 caaatgaata tttaagtttg aaaaaccaaa taaataaagc aagttggcca ccaaaaaagg
    22561 caaggtaaat cgtgttcgca gatgtcgcta aaatttctct accaacacgc atgccagatt
    22621 ggaataattc gtattgcgta agtgttggat tcacttgatg caattcataa atgggtgaac
    22681 taatggtaat cgttaaatct atcacagctg caataacagc aagaataata gtgaacacca
    22741 taaattgaat catatcaatg ccaatattca ttgaatacac atatgtttca tcttgttgtt
    22801 cggttgaaaa gccttgtaga tgaccgaagt agaccgataa ataaatgagt gtaatcaaca
    22861 atattgttgt aacgatagtg ctgataaatg cagcttgtgt tttaacattg taactattga
    22921 gtacgaataa attacaagcg ccaataataa tgcagaaaaa gaatgtgacg acataaatcg
    22981 gtacgccaaa aataatcaat acaatactaa taattaaaat agcgaaattt aaaaataggg
    23041 ttaaataaga gatgaatccc tttttacctc cgaaaattat catcagaaag aggagcaata
    23101 acgccaatat aaatacagca ttcattgttt cgccctcctt aatgtttcaa atatttccat
    23161 aaacaatatt gtgataggaa ttgtaagtac gatacctata ccgcctgtta gtgcgcgcgc
    23221 aatttctaac gaccaattca tcgaaatagt ataagtcact gtattggcat tttttaaaaa
    23281 gattaaaaac ataggtagtg caccggataa atatgagaat aataagatgt tagtcattgt
    23341 tcccataata tcttggccta tgtttcgccc agcaagcgcc catctcctca ttgaaatgtg
    23401 cggcgtacgc tgtaaaattt catgcatacc actagcaatt gtaattgcaa catccataat
    23461 agcgccaagt gaacctatta acactgaggc taggaagata tctttcggtg gtaatgataa
    23521 aaagttcatc gtttcatatt taatgccttt accatctgtc atatatatga ttaattctgt
    23581 taaacctata ctcaaaaaag ttccgataat tgtactggct atggtaatga gtgtacgcat
    23641 atgccagcct gtaacgagca ataaagtgag tattgttgaa cagatcatgg caatggtcat
    23701 gagtaagaat aaattaatat tgctatgttg aatatgaatg taaattgcga ttaatatggc
    23761 aatagaattc aagattaatg ataaaatcga ttgcagtccg actttgcgac caaccaataa
    23821 tacagttaat aagaacaaac cagtgatgat aaccgttaag gtatcacgct tcttttctat
    23881 aatataagca tcactcggct tgttagaaat atgtaataat actttttcgt gtgtgcgaaa
    23941 tgcctcagaa tctgcttgcg atttgacgta ctgatgatta atcgtcgtcg tttctccagc
    24001 aaattgacca tttaatattt tgacttttaa ttgattttta tatttaatat cacgattatt
    24061 ttgtgcatct tttgtaggtg tcgaagaaac atgtttgaca tctataattt gaccaattgg
    24121 tttgttgtaa aagttctcat tattgaatgt gaataaaata gcaccaatga atgcgatgca
    24181 gaacaaacct aaaattatat taaatggctt tgtaaataaa tttctatatt tcaaaaacaa
    24241 aaccccaatt ctatgaatga attaatatgg tgattatacg cccttaattt tttattttca
    24301 aagatattac tgtgaaatgt ataacgaaaa tcatcattga tagagtcgaa tggcttaatg
    24361 gaatgtagac gttttagtca ttaattgttg aatgagtgtt aataagatac caatatcact
    24421 cttcgtataa ggttctttcg taatagcaca tatcgttctt tttaattcag tatggtctaa
    24481 ttttatatct atacatgatt tagattctgg taaatgtata ttttgtgatg aaatgatgta
    24541 accttctttt tgacgaagga gatactgcgc aagtggttgg ctactgattg tgtatacatc
    24601 tgatttagta atcttacgca attgtttttt tacagattcg gcaaatggtc ccaagcaata
    24661 aatatgacta tgctcaaact gaattaatgg tgggtgtgtc gccatcgtga ttggatcgtc
    24721 tgaaggcgca tataaatgat agtgctcttc gaataaaggt agcatatgta attgtttgtg
    24781 tttacgtatt tctggtgtaa gttccgtgaa accaatgtct atatttccat ttaatacgct
    24841 atttataatt gtgtcatgtt ctaataaatt agggatgaca tgtgtatcat tttgtaaatg
    24901 aaacgtttgg ataagtggta gtaacatgtg ggatacgtca ctctcattat agccaatgta
    24961 gatgctttta tttttagtta atccatgact ttgaaattgt tcaatcgtgc tatctaaatg
    25021 ttcaataata cgcagagctt cattaaataa taatttccct tcagatgtga gcgtaatatt
    25081 acgtccttgc tttttaaata atgacacatt aagttcttgt tctaataatg taatttgacg
    25141 gcttatcgct gattgagcaa tgtttagttc aagtgctgtt tcggagatat gttctctttt
    25201 agcgacctcg ataaaatatc ttaattgttt aatttccata gcgatatagg cacctccaaa
    25261 aatgagtgtt ttgtaactat tatagcaata ttattgataa atgttctatt ttttagatga
    25321 atatcttcta ttttatatat tgaacagata aattttttag attatagtaa ttatcattaa
    25381 taactaatat cagaatattc taaaaaaagg ggtgtgcatc atgcacaatg agaaattaat
    25441 taaaggctta tatgactatc gtgaggaaca tgatgcgtgt ggtattggtt tttatgcgaa
    25501 tatggacaat aaaaggtctc acgacatcat tgataaatcg cttgaaatgt tgcgacgttt
    25561 agatcacagg ggcggggtcg gcgcagatgg catcactggt gatggcgcag gtattatgac
    25621 tgaaatacct tttgcatttt tcaaacaaca tgtaacggac tttgatatcc caggtgaagg
    25681 tgaatatgcc gtggggttat ttttttccaa agaacgcatt ttaggttctg aacatgaagt
    25741 agtttttaaa aaatattttg aaggcgaagg gttatcaatt cttggttatc gtaatgtacc
    25801 agttaataaa gatgccattg ctaaacatgt agcagatacg atgccagtca ttcaacaagt
    25861 gtttattgat attagggaca ttgaagatgt tgaaaagcat ttgtttttag cgagaaaaca
    25921 attagagttc tattcgactc agtgcgattt agaattgtat tttacgagct tatcacgcaa
    25981 aacaattgta tataaaggtt ggttacgatc agaccaaatt aaaaaattct atacagattt
    26041 atcggatgat ttatatcaat caaagctagg gttagtgcat tcgagattta gtacgaatac
    26101 attcccgagt tggaaaaggg cgcatcctaa ccgtatgtta atgcataatg gtgagattaa
    26161 cacgattaaa ggtaatgtga actggatgcg agcacgtcaa cataaattaa tcgaaacatt
    26221 atttggtgag gatcaacata aagtgtttca aattgtcgat gaggatggta gtgactctgc
    26281 tattgtagat aatgcgctag agttcttatc gttagccatg gagccagaaa aggcagcgat
    26341 gctactcata cctgaacctt ggttatataa tgaagcgaat gatgcaaatg tacgtgcgtt
    26401 ttatgaattt tatagttatt taatggagcc gtgggatggt cctacaatga tttcgttctg
    26461 taacggtgac aaacttggcg cgcttacaga tagaaatgga ttacgtccag gtcgttatac
    26521 gattactaaa gataacttta ttgtcttttc atctgaagtg ggtgttgtgg acgtacctga
    26581 aagtaatgtt gcttttaaag gtcaattgaa tcctggaaag ttattgcttg ttgattttaa
    26641 acagaataaa gtcattgaaa ataatgattt aaaaggtgcg attgccggag aattaccata
    26701 taaagcgtgg attgataacc ataaagttga ttttgatttt gaaaatatac agtatcaaga
    26761 ttcgcaatgg aaagatgaga cgttatttaa attacaacgt caatttgcat acacgaaaga
    26821 agagattcat aagtatattc aggaacttgt agaaggtaag aaggatccta tcggtgcaat
    26881 gggatatgat gcgccaattg cagtgttgaa cgagcgacca gaatcactat ttaattactt
    26941 taaacagctg tttgcacaag ttacgaatcc accaattgat gcgtatcgtg aaaaaatcgt
    27001 aacgagtgaa ctttcttatt taggtggcga aggtaactta ctagcacctg acgaaacggt
    27061 tttagatcgt attcaattga aaagaccggt attgaatgaa tcacacttag cagcgattga
    27121 tcaggagcat tttaaattaa catatttatc aacggtatat gaagggaatt tggaagatgc
    27181 gttagaagta ttaggccgag aagcagtgga tgctgtaaag caaggcgctc aaattctagt
    27241 gttagatgat agtggattag ttgatggcaa tggctttgca atgccgatgt tactcgcaat
    27301 aagtcatgtg catcaattac ttattaaagc agatttacgt atgtctacaa gtttagtcgc
    27361 taaatctggt gagacacgag aagtgcatca tgttgcttgt ttactcgcat atggagcgaa
    27421 tgcaattgtg ccatacctag cgcaacgtac agttgaacaa ctgacattga cagaagggtt
    27481 acaaggcacc gttgtcgata atgttaagac atatacggat gtattgtcgg aaggtgttat
    27541 taaagtaatg gcgaagatgg gaatttcgac agtgcaaagt tatcaaggtg cacaaatatt
    27601 tgaagcgatt ggcttgtctc atgatgtgat tgatcgttat tttactggaa cacagtctaa
    27661 gttatctggt atttcgattg atcaaattga tgctgaaaat aaagaacgtc aacaaagtga
    27721 tgataattat cttgcatcag gtagtacatt ccaatggaga caacaaggtc aacatcatgc
    27781 ttttaatccg gaatctattt tcttattgca gcacgcatgt aaagaaaatg actatgcgca
    27841 atttaaagca tactctgaag cggtaaacaa aaatagaaca gatcacatta gacatttact
    27901 tgaatttaaa gcatgtacac cgattgacat cgaccaagtt gaaccggtaa gtgacattgt
    27961 caaacgtttt aatacagggg cgatgagtta tggatcgatt tcagcggaag cacatgaaac
    28021 gttagcacaa gccatgaacc aattaggtgg aaagagtaat agtggtgaag gtggcgaaga
    28081 tgcaaaacgt tatgaagtac aagttgatgg aagcaacaaa gtaagtgcga ttaaacaagt
    28141 tgcttctgga cgttttggtg taactagtga ttatttacaa catgccaaag aaattcaaat
    28201 taaagttgcg caaggtgcaa agcctggtga aggtggtcaa ttacctggta ctaaggtata
    28261 tccgtggatt gcgaagacaa gagggtcaac gccaggtatc ggtctgattt caccaccgcc
    28321 acatcatgat atttattcaa tagaagattt agcgcaactg atacatgatt tgaaaaatgc
    28381 gaataaagat gcagatatcg cggtaaaatt agtttcgaaa acaggtgttg gtaccattgc
    28441 atctggggtg gcgaaagcat ttgcagataa aattgtcatc agtggttacg atggtggtac
    28501 aggggcttca cctaaaacga gtatacagca tgccggtgtt ccttgggaga ttggtttagc
    28561 agaaacacat caaacattaa aactaaatga cttaagaagt cgtgttaagt tagaaacaga
    28621 cggtaagtta ttaactggta aagatgtagc gtacgcatgt gcgcttggag cggaagaatt
    28681 tggatttgca actgcaccat tagtggtgtt gggctgtatt atgatgcgtg tatgccataa
    28741 agatacatgt ccagtaggag ttgcaactca aaacaaagat ttacgtgctt tatatagagg
    28801 taaagcacat catgttgtta attttatgca ttttattgca caagaattaa gagaaatttt
    28861 agcatcttta ggtttgaaac gtgtagaaga cttagttgga agaactgatt tattacaacg
    28921 atcatcaaca ttaaaagcga atagcaaagc ggctagtatt gatgttgaaa aactgttatg
    28981 tcctttcgat gggccaaaca caaaagaaat tcaacaaaat cataatcttg agcatggatt
    29041 tgatttaaca aatttatatg aggtaacgaa gccatatatt gctgaagggc gtcgctatac
    29101 aggtagcttt acagtaaata atgaacaacg tgatgtaggg gttattacag gtagtgagat
    29161 ttcgaaacaa tatggagaag caggacttcc tgaaaataca attaatgttt atacgaatgg
    29221 tcatgctggt caaagtcttg cagcatatgc accgaaaggc ttaatgattc atcatactgg
    29281 agatgcgaat gactatgttg gtaaaggatt atctggtggt acggtcattg tcaaagcacc
    29341 ttttgaagaa cgacaaaatg aaattattgc tggtaacgtc tcattctatg gtgcgacagg
    29401 tggtaaggca tttattaacg gtagtgcagg agaaagattc tgtattagaa atagtggtgt
    29461 agatgttgtc gttgaaggta tcggcgacca tggattagag tacatgactg gtggacatgt
    29521 cattaattta ggtgatgtag gtaagaactt cggtcaaggt atgagtggtg gtattgctta
    29581 cgttatcccg tctgatgtag aagcttttgt tgaaaataat caactagata cgctttcgtt
    29641 tacaaagatt aaacaccaag aagaaaaagc attcattaag caaatgctgg aagaacatgt
    29701 gtcacatacg aatagtacga gagcgattca tgtgttaaaa cactttgatc gcattgaaga
    29761 tgtcgtcgtt aaagttattc ctaaagatta tcaattaatg atgcaaaaaa ttcatttgca
    29821 caaatcatta catgacaatg aagatgaagc gatgttagct gcattttacg atgacagtaa
    29881 aacaatcgac gctaaacata aaccagccgt tgtgtattaa ggaaaggggg agatacgatg
    29941 ggtgaattta aaggatttat gaagtatgac aaacagtact taggtgaatt atcactggta
    30001 gaccgtttga agcatcataa agcatatcaa caacgattta ctaaagaaga tgcctctatc
    30061 caaggtgcgc gatgtatgga ttgtggaacg ccgttttgtc aaaccggaca acagtatggt
    30121 agggaaacaa taggttgtcc aattggaaac tacattcctg aatggaacga ctttgtgtat
    30181 catcaagatt ttaaaactgc ttatgaacgc ttaagcgaaa caaataactt tcctgacttt
    30241 acagggcgtg tatgtcctgc accatgcgaa agtgcttgtg tgatgaagat taatagagaa
    30301 tcgattgcga ttaaaggtat tgaacgcaca attattgatg aagcttttga aaatggttgg
    30361 gtagcgccga aagttccgag tcgccgtaga gatgaaaaag tggcaatcgt tggaagcggt
    30421 ccagcaggat taactgctgc tgaagaactt aatctactag gatatcaagt gactatttat
    30481 gaacgtgcta gagaatcagg cggtttatta atgtatggta tacctaatat gaagcttgat
    30541 aaagatgtgg ttcgacgtcg tattaagtta atggaagaag cgggcattac tttcgttaat
    30601 ggtgttgaag taggcgttga tattgataaa gcaacgttag aatctgagta tgatgccatt
    30661 atattatgta ctggtgcaca aaaaggtaga gatttacctt tagaaggacg catgggtgat
    30721 ggtatacatt tcgctatgga ttatttaact gaacaaacgc aactgttaaa tggagaaatt
    30781 gatgatataa caataactgc aaaagataag aatgtcatta tcattggtgc tggtgataca
    30841 ggggcagact gtgtagcgac agcattaaga gaaaattgta agtcgattgt tcaatttaat
    30901 aaatatacga aattgccaga agcaattacg tttacagaaa atgcatcatg gcctttagca
    30961 atgccggtgt ttaaaatgga ctatgcgcac caagagtacg aagctaagtt tggtaaggaa
    31021 ccacgtgcat atggtgttca aacaatgcgt tacgatgttg acgataaagg acatatacgt
    31081 ggtttgtata ctcaaatttt agagcaaggc gaaaatggta tggtcatgaa agaaggacct
    31141 gaaagatttt ggcctgctga ccttgtatta ttatcaatcg gcttcgaagg tacagaacca
    31201 acagtaccga atgcttttaa cattaaaacg gatagaaatc gaatcgtggc tgatgataca
    31261 aactatcaaa ctaataatga aaaagtattt gctgctggag atgctagacg tggtcaaagt
    31321 ttagttgtat gggcgattaa agaaggtaga ggcgtagcga aagcagtaga tcagtattta
    31381 gcgagtaaag tttgtgtata atctttgtgt gacaatgatg atgttcgcgt tgacgttgta
    31441 tggtgctgaa ttgaaaagga agcgacttag tatctatcaa cgtcacatgc catctttgta
    31501 acctaaaaat aaaggtttgt aagacaacta atagattaat tataagtagt gattttttac
    31561 attcgtttta taggtcaact gtagtggaag acaatgattt gtggtaatca tgtaatgctt
    31621 aaaaacaata ttgactttta cagaacgttc atatatgata aatattgtgt ttaggaggaa
    31681 tacccaagtc cggctgaagg gatcggtctt gaaaaccgac aggggcttaa cggctcacgg
    31741 gggttcgaat ccctcttcct ctgccatcaa tatttatatt aaatactata tataatgaag
    31801 gtaagtgctc aaattttgag tatttacctt ttttatttgt ctttgaatgg gtcgtatttt
    31861 tggctataga aatgataagg cattgaaata gaaaggacat ttggcttttg aatgacacat
    31921 ggctgaatgt cttttttatt tggtgatgta cgtaggagtc tttgcttggg catggaaatt
    31981 actatttggg tatgggtatg ataatacagc ttattcttta acatagtgta tgcgtttgga
    32041 ggttatggat aatgctattg tagcgaaaat tcatgctata aatgtagtcg tagccatttg
    32101 attttcttaa atatctaaac tagataattg tgaaatatta aaatattttc atgaaagcct
    32161 ttacataact tgtctagaca agttatactc tttttaagac attaagggag tgaagttcat
    32221 ggctgtaaaa agagaagatg taaaagccat cgttaccgca attgggggaa aggataatct
    32281 agaagctgca acacattgtg taacaagatt gcgtttagta cttaaagatg aaagtaaagt
    32341 tgataaggaa gcgttaagta ataatgcttt agttaaaggg cagttcaaag cagaccatca
    32401 atatcaaatt gtcattggtc caggaacagt cgatgaagtg tataagcagt ttattgatga
    32461 aacaggtgct caagaagctt ctaaagatga agcgaaaaaa gcagctgcgc aaaaagggaa
    32521 tccagtacaa cgtttgatta aattgttggg ggatattttt ataccaatat tacctgcgat
    32581 tgtgacagct ggtttgttaa tggggatcaa taatttactt acaatgaaag gattatttgg
    32641 tccgaaagaa ctcattgtga tgtatccaca aatagctgat atttcaaata tgataaacgt
    32701 catagcaaac acggcattta ttttcttacc agcattaatt ggttggagta gtatgcgtgt
    32761 atttggtggt agtccgattc taggtttagt cttaggtttg attttaatgc acccgcaatt
    32821 agtatctcag tatgatttgg caaaaggtaa tattccgacg tggaatttat ttggcttaga
    32881 gattaagcag ttgaattacc aaggtcaagt gttgcctgtt ttaattgcag cttatgttct
    32941 agctaaaatt gaaaaaggat taaataaagt tgttcacgat tcgataaaaa tgttggtcgt
    33001 tggacccgta gcgcttttag ttactggatt tttagcattt attatcattg gaccagttgc
    33061 attattgatt ggtacaggta ttacatctgg tgttacattt atattccaac atgctggttg
    33121 gttaggtggc gcaatttatg gtttgctata tgcaccgctt gtaattactg gcttgcatca
    33181 tatgttttta gcagtagatt tccaattaat gggtagcagt ttaggcggta catacttatg
    33241 gccaattgtt gcaatctcaa atatttgtca aggttcagca gcatttggtg catggtttgt
    33301 ctataaacga cgtaaaatgg ttaaagaaga aggtttagca ttaacatctt gtatttctgg
    33361 tatgttaggt gttacggagc cagccatgtt tggtgtgaac ttacctttaa aatatccatt
    33421 tatcgccgca atatcaacgt cttgtgtatt aggggcaatc gttggtatga ataatgtact
    33481 tggaaaagtt ggtgttggtg gtgtaccggc attcatttca attcaaaaag aattttggcc
    33541 tgtatatctt gttgtaacag gaattgcaat tgttgtacca tgtatattaa cgattgtgat
    33601 gtctaatttt agtaaacaaa aagcaaaaga aattgttgaa gattaataaa ataaaaaagg
    33661 ggcgttcgtt atttggacgc cttttattat gttataaggt ggtcatcgtg tgttgaaaga
    33721 aatagattgg agaaagtcag tagtatatca aatatatcct aagtcattta atgatacgac
    33781 aggaaatggt ataggtgata tcaacggtat tatagaaaaa ttagattata tcaaattgtt
    33841 aggtgttgat tatatttggt taacaccagt gtatgaatcg ccaatgaatg ataatggtta
    33901 tgatataagc aattatttag aaataaatga ggacttcggt acgatggatg attttgaaag
    33961 gttaattaat gttgcgcatg aaaaaggtat taaagtgatg ttagatattg taatcaatca
    34021 tacatcgacg gagcatgaat ggtttaaagc ggctcgtaaa tctaaagaca atccatatag
    34081 agattattac ttttttaaag catctgaaga tgggcctcca acaaattggc attccaaatt
    34141 cggaggtaat gcatggaagt atgattctga gacagatgaa tattatttac atttatttga
    34201 tgtcagtcaa gctgatttaa attgggataa tccggaagta cgtcaatcgt tatatcgcat
    34261 tgtcaatcat tggatagact tcggcgttga tggttttcga tttgatgtca ttaacttaat
    34321 ttctaaaggt gaatttaagg actctgacaa aataggtaaa gaattttata cggatggtcc
    34381 tagagtgcat gagtttctgc atgaattaaa tcgtcaaacg tttggtaaca ctgacatgat
    34441 gactgtagga gaaatgtctt cgacgacgat tgaaaattgt attaagtata cacagccaga
    34501 acgccaagaa ttgaatagtg tttttaattt tcatcatcta aaggttgatt atgttgatgg
    34561 tgaaaagtgg acaaatgcga agcttgattt tcataagtta aaggaaattc tgatgcaatg
    34621 gcaacgaggt atttatgacg gtggcggatg gaacgcgatt ttctggtgta atcatgatca
    34681 gccacgggta gtgtctagat ttggtgatga tacgtcggaa gagatgagga tacaaagtgc
    34741 taaaatgtta gctatcgcac tgcatatgtt gcaagggacg ccatatattt accaaggtga
    34801 agaaattggt atgacggacc cacattttac atcaatagca caatatcgtg atgttgaatc
    34861 gattaatgcc taccatcagt tgttaagtga agggcatgct gaagcggatg tgttagcgat
    34921 tttaggacag aagtcacgag ataattcgag aacgcctatg caatggagtg atgatgttaa
    34981 tgctggattt acagctggta agccttggat tgatatttcg gaaaattatc atcaggtcaa
    35041 cgttagacaa gcacttcaga ataaagattc tattttctat acgtatcaaa aattaataca
    35101 attaagacat acgcatgata ttattacgta tggagacatt gtgccacgtt ttatggatca
    35161 tgatcattta tttgtttatg aacgtcatta taagaatcaa caatggctag taattgcgaa
    35221 tttctcagca tcggctgttg atttgccaga aggattggcg tgcgaaggtc gtgttgtgat
    35281 tcaaactggt acagtggaaa ataatacgat aagcgggttt ggtgcaattg taatcgaaac
    35341 aaacgcgtaa aataaattga gtggatgcgt ttatatggcg aaacaaaaaa agtttatgaa
    35401 gatttatgag gcgttgaaag aagatatatt aaacgggcag attcaatatg gtgaacaaat
    35461 tccgtctgaa catgatttgg tgcaattgta ccagtcatct cgagagaccg tgcgtaaggc
    35521 attagatttg ttggcattag acggcatgat tcaaaagatt catggtaaag ggtcacttgt
    35581 catttatcaa gaggttacag agtttccatt ttctgaactt gttagtttta aagaaatgca
    35641 agaagaaatg ggtgtcgcat atttaactga agttgttgtg aatgaggttg ttgaagcgca
    35701 tgaagtgcca gaagttcaac atgctttaaa catcaattct agtgaatcac tcattcatat
    35761 tgttagaact cgtcggctta accaacatgt gaagattgtt gatgaagatt attttctaaa
    35821 gtcgattgtt tcagacatag gtaatgatgt tgcgagtgat tctatttatg attatttgga
    35881 aaaggtatta aatcttaata ttagttattc aagtaagtct attacttttg aaccgtttga
    35941 tgaacaagca tatcaattgt ttggtgatgt atcagtggct tattcagcaa cagttcgaag
    36001 tattgtgtat ttagaaaata caatgccgtt tcaatataat atttcaaaac atcttgcaaa
    36061 tgaatttaaa tttaatgact tctcaagacg tcgtacaaag taaacaatga tataaatgat
    36121 ttatacttgc aattaactat taaaatatag taatatatat cttgccgtgc taggtgggga
    36181 ggtagcggtt ccctgtactc gaaatccgct ttatgcgagg cttaaatcct ttgttgaggc
    36241 cgtatttttg cgaagtctgc ccaaagcacg tagtgtttga agatttcggt cctatgcaat
    36301 atgaacccat gaaccatgtc aggtcctgac ggaagcagca ttaagtggat catcatatgt
    36361 gccgtagggt agccgagatt tagctaacga ctttggttac gttcgtgaat tacgttcgat
    36421 gcttaggtgc acggtttttt atttttaaaa atattaaacc gattattaag agttgaaaat
    36481 atataattat agaagctact ttcttgaaga caattcagcg tattatacgt ggaacatgtt
    36541 tgtgggaagt agctttttta tatatgaagt ttgattcaag tggaatggat gcgcagtttg
    36601 aatgattttt tacggatata aaaagtaaga agttattatt tgatgataaa gaaatgatgg
    36661 tgaaatgagg gggagtacct tacaatagaa ttattaatga gatacgttat gattattgac
    36721 aatcaaatgc ctacggagga catatgcaaa tatatttaag tactttaaca gagttagatt
    36781 atgataaatc tttaaatagt attgaagaaa gttttgatga taatcctgaa acgagttggc
    36841 aagcacgtgc gaaagtaaaa catttaagaa aatctccttg ctataatttt gaattagaag
    36901 taattgcgaa aaatgaaaat aacgatgtcg ttggacacgt tttattaatt gaagtagaaa
    36961 tgaatagtga tgataagacg tattatggtt tggcgattgc ttctttatca gtcaaccctg
    37021 aattgcgtgg acaaaaatta ggtcgtggct tggttcaagc agtagaagag cgtgcaaaag
    37081 cgcaagagta tagtacggtt gttgtagacc attgttttga ctactttgaa aagttgggtt
    37141 atcaaaatgc tgctgagcat gacattaaat tagaatctgg tgatgcaccg ttacttgtaa
    37201 aatatttatg ggataatttg acggatgcac cacacggaat cgtaaaattt ccagaacatt
    37261 tttattaatt gttcatttaa gaagtaaagg tattatcatg ctataatgaa aggtaattgt
    37321 ttatggaggt gctaacttga attatcaagc cttatatcgt atgtacagac cccaaagttt
    37381 cgaggatgtc gtcggacaag aacatgtcac gaagacattg cgcaatgcga tttcgaaaga
    37441 aaaacagtcg catgcatata tttttagtgg tccgagaggt acggggaaaa cgagtattgc
    37501 caaagtgttt gctaaagcaa tcaactgttt aaatagcact gatggagaac cttgtaatga
    37561 atgtcatatt tgtaaaggca ttacgcaggg gactaattca gatgtgatag aaattgatgc
    37621 tgctagtaat aatggcgttg atgaaataag aaatattaga gacaaagtta aatatgcacc
    37681 aagtgaatcg aaatataaag tttatattat agatgaggtg cacatgctaa caacaggtgc
    37741 ttttaatgcc cttttaaaga cgttagaaga acctccagca cacgctattt ttatattggc
    37801 aacgacagaa ccacataaaa tccctccaac aatcatttct agggcacaac gttttgattt
    37861 taaagcaatt agcctagatc aaattgttga acgtttaaaa tttgtagcag atgcacaaca
    37921 aattgaatgt gaagatgaag ccttggcatt tatcgctaaa gcgtctgaag ggggtatgcg
    37981 tgatgcatta agtattatgg atcaggctat tgctttcggc gatggcacat tgacattaca
    38041 agatgcccta aatgttacgg gtagcgttca tgatgaagcg ttggatcact tgtttgatga
    38101 tattgtacaa ggtgacgtac aagcatcttt taaaaaatac catcagttta taacagaagg
    38161 taaagaagtg aatcgcctaa taaatgatat gatttatttt gtcagagata cgattatgaa
    38221 taaaacatct gagaaagata ctgagtatcg agcactgatg aacttagaat tagatatgtt
    38281 atatcaaatg attgatctta ttaatgatac attagtgtcg attcgtttta gtgtgaatca
    38341 aaacgttcat tttgaagtat tgttagtaaa attagctgag cagattaagg gtcaaccaca
    38401 agtgattgcg aatgtagctg aaccagcaca aattgcttca tcgccaaaca cagatgtatt
    38461 gttgcaacgt atggaacagt tagagcaaga actaaaaaca ctaaaagcac aaggagtgag
    38521 tgttgctcct actcaaaaat cttcgaaaaa gcctgcgaga ggtatacaaa aatctaaaaa
    38581 tgcattttca atgcaacaaa ttgcaaaagt gctagataaa gcgaataagg cagatatcaa
    38641 attgttgaaa gatcattggc aagaagtgat tgaccatgcc aaaaacaatg ataaaaaatc
    38701 actcgttagt ttattgcaaa attcggaacc tgtggcggca agtgaagatc acgtacttgt
    38761 gaaatttgag gaagagatcc attgtgaaat cgtcaataaa gacgacgaga aacgtagtag
    38821 tatagaaagt gttgtatgta atatcgttaa taaaaacgtt aaagttgttg gtgtaccatc
    38881 agatcaatgg caaagagttc gaacggagta tttacaaaat cgtaaaaacg aaggcgatga
    38941 tatgccaaag caacaagcac aacaaacaga tattgctcaa aaagcaaaag atcttttcgg
    39001 tgaagaaact gtacatgtga tagatgaaga gtgatacatg acaagcgata taatcgtatg
    39061 tataatgaaa gaaacatcat tttattgata aatatttatt gattttcaag gaggaaatgg
    39121 aatatgcgcg gtggcggaaa catgcaacaa atgatgaaac aaatgcaaaa aatgcaaaag
    39181 aaaatggctc aagaacaaga aaaacttaaa gaagagcgta ttgtaggaac agctggcggt
    39241 ggcatggttg cagttactgt aactggtcat aaagaagttg tcgacgttga aatcaaagaa
    39301 gaagctgtag acccagatga tattgaaatg ctacaagact tagtgttagc agctactaat
    39361 gaagcgatga ataaagctga tgagcttact caagaacgtt taggtaaaca tactcaaggc
    39421 ttaaacatcc ctggaatgtg atcatagatg cattatccag aacctatatc aaagcttatt
    39481 aatagcttta tgaaattgcc aggcattggt ccaaagacag cccaacgtct ggcttttcat
    39541 accttagata tgaaagaaga cgatgttgtt cagtttgcca aagcattagt agatgttaaa
    39601 agagaattaa catattgtag cgtatgtggt cacattactg aaaatgatcc atgttatatt
    39661 tgtgaagata agcaaagaga tcgttcagtt atttgtgttg tggaagatga caaagatgtc
    39721 atagctatgg aaaaaatgag agaatacaaa ggtttatatc acgttttaca tgggtctatt
    39781 tcgcctatgg atggaattgg accagaagat attaatattc cttcattgat tgaacgcttg
    39841 aaaaacgatg aagttagcga attaatctta gctatgaacc cgaacttaga gggggaatct
    39901 acagccatgt atatttctag attagttaag cctataggta tcaaagtgac gagattagca
    39961 caagggttat ctgtaggtgg cgatttagag tatgctgacg aagtaacatt atctaaagca
    40021 atcgcaggta gaacagaaat gtaatgtctt ctattaaaca ttttagaatt taatactata
    40081 gtaagaaaag tcacagtgta atcattgtgg ctttttttat ggtgtggtgt actactttat
    40141 ttgtggtgtg acggtggcat ggtttaccta gttttactga ggggtgggga atctttagga
    40201 agcaaaccat tggttgtgat ttgttatttc taatagtaat gaagtgaatt ggattatcga
    40261 agtagatcta tggttatggt gtgttggtgg catggtttac ctggttttac tgagggatgg
    40321 gtaatcttaa agaagcaaat cgttggttgt gatttgttac ttctaatagt aatgattcga
    40381 attgaatcat cgaagtagga atactgttgt ggtgtgttgg tgctattaat ttgataaatg
    40441 cggttgatgg ttaatgacta ggcaaatgaa attcttttgt aattgaaatg atagatgctg
    40501 gcttagtagt tgtacttctt tggtctaaag cttattaaat cagcctgtaa agcggtgttt
    40561 tgagagatta tttaaaactt gtaaatttat ttttaatttc tggtaaaaaa ataaagttct
    40621 gttttgcggt tttttcgatt gatatattta gagaaaaacc tgtttcttgt tctaaaaaac
    40681 gtactatcta taagtggggg ttttttaagt tcgattttta gaatgagagc gttcagaatg
    40741 gatagtaaag gtgtaatttt tactgttgtt aagcagtttg aaagcctgta tagtatttat
    40801 ttgttgaggc aaacaaaaca actcaactta agaaataact tgaattacta acgaaaatta
    40861 attttaaaaa gttgttgact taaatgttaa taaaatgtat aattaattct tgtcggtaag
//
LOCUS       DAKSQE010000009        40472 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.009, whole genome shotgun sequence.
ACCESSION   DAKSQE010000009 DAKSQE010000000
VERSION     DAKSQE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 40472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40472
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.009"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            67..1272
                     /locus_tag="O0V33_000345"
     CDS             67..1272
                     /locus_tag="O0V33_000345"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCX8975030.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMRAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAM
                     LHGGYSNEQ"
     gene            1262..2641
                     /gene="argH"
                     /locus_tag="O0V33_000346"
     CDS             1262..2641
                     /gene="argH"
                     /locus_tag="O0V33_000346"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCX8975031.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPGYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLSTYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            2883..3812
                     /locus_tag="O0V33_000347"
     CDS             2883..3812
                     /locus_tag="O0V33_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCX8975032.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNHIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DENVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNKNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(4207..5451)
                     /locus_tag="O0V33_000348"
     CDS             complement(4207..5451)
                     /locus_tag="O0V33_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HCX8975033.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVNEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(5560..6750)
                     /locus_tag="O0V33_000349"
     CDS             complement(5560..6750)
                     /locus_tag="O0V33_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCX8975034.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINKNTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFARDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            complement(7058..8185)
                     /locus_tag="O0V33_000350"
     CDS             complement(7058..8185)
                     /locus_tag="O0V33_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCX8975035.1"
                     /translation="MKSKYEPLFDKVVLPNGVELRNRFVLAPLTHISSNDDGTISDIE
                     LPYIEKRSQDVGITSNAASNVSDVGKAFPGQPSIAHDSDIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELTPDGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPIAVIEEVLKSKEVYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATAREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADDALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            complement(8547..8924)
                     /locus_tag="O0V33_000351"
     CDS             complement(8547..8924)
                     /locus_tag="O0V33_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCX8975036.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
     gene            complement(9339..9932)
                     /locus_tag="O0V33_000352"
     CDS             complement(9339..9932)
                     /locus_tag="O0V33_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCX8975037.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            9996..10379
                     /locus_tag="O0V33_000353"
     CDS             9996..10379
                     /locus_tag="O0V33_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HCX8975038.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHERKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            10510..12915
                     /gene="mnhA1"
                     /locus_tag="O0V33_000354"
     CDS             10510..12915
                     /gene="mnhA1"
                     /locus_tag="O0V33_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HCX8975039.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWIWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASKANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTVFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            12908..13336
                     /gene="mnhB1"
                     /locus_tag="O0V33_000355"
     CDS             12908..13336
                     /gene="mnhB1"
                     /locus_tag="O0V33_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HCX8975040.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            13336..13677
                     /gene="mnhC1"
                     /locus_tag="O0V33_000356"
     CDS             13336..13677
                     /gene="mnhC1"
                     /locus_tag="O0V33_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HCX8975041.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            13670..15166
                     /gene="mnhD1"
                     /locus_tag="O0V33_000357"
     CDS             13670..15166
                     /gene="mnhD1"
                     /locus_tag="O0V33_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HCX8975042.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPLRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTI
                     GIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVFFEVFLMSSYFLLVIGSTKIQLQE
                     TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIF
                     VFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAITRTLSLFFSDNVSFSH
                     YVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYT
                     LHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSG
                     FYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVK
                     YLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            15168..15647
                     /gene="mnhE1"
                     /locus_tag="O0V33_000358"
     CDS             15168..15647
                     /gene="mnhE1"
                     /locus_tag="O0V33_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HCX8975043.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            15647..15940
                     /gene="mnhF1"
                     /locus_tag="O0V33_000359"
     CDS             15647..15940
                     /gene="mnhF1"
                     /locus_tag="O0V33_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HCX8975044.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            15918..16274
                     /gene="mnhG1"
                     /locus_tag="O0V33_000360"
     CDS             15918..16274
                     /gene="mnhG1"
                     /locus_tag="O0V33_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HCX8975045.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            16537..17691
                     /locus_tag="O0V33_000361"
     CDS             16537..17691
                     /locus_tag="O0V33_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCX8975046.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNS
                     YTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMDHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKEHDQVVYDKTDHIVGSGITAAHLALKLLNHDDDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISDGLINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLYVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(17747..18121)
                     /locus_tag="O0V33_000362"
     CDS             complement(17747..18121)
                     /locus_tag="O0V33_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HCX8975047.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVIATAEIIHQGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(18140..19456)
                     /locus_tag="O0V33_000363"
     CDS             complement(18140..19456)
                     /locus_tag="O0V33_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HCX8975048.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            complement(19867..21342)
                     /locus_tag="O0V33_000364"
     CDS             complement(19867..21342)
                     /locus_tag="O0V33_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HCX8975049.1"
                     /translation="MNFKLNNTISNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKTRSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVEVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKYDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVYYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSKVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            complement(21473..22681)
                     /locus_tag="O0V33_000365"
     CDS             complement(21473..22681)
                     /locus_tag="O0V33_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCX8975050.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
     gene            complement(23135..23494)
                     /locus_tag="O0V33_000366"
     CDS             complement(23135..23494)
                     /locus_tag="O0V33_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HCX8975051.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKKDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(23507..23743)
                     /locus_tag="O0V33_000367"
     CDS             complement(23507..23743)
                     /locus_tag="O0V33_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HCX8975052.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
     gene            24060..25124
                     /locus_tag="O0V33_000368"
     CDS             24060..25124
                     /locus_tag="O0V33_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCX8975053.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
     gene            complement(25184..25507)
                     /locus_tag="O0V33_000369"
     CDS             complement(25184..25507)
                     /locus_tag="O0V33_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HCX8975054.1"
                     /translation="MEHVSVIVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            25608..25850
                     /locus_tag="O0V33_000370"
     CDS             25608..25850
                     /locus_tag="O0V33_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HCX8975055.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            complement(26112..27287)
                     /gene="dltD"
                     /locus_tag="O0V33_000371"
     CDS             complement(26112..27287)
                     /gene="dltD"
                     /locus_tag="O0V33_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCX8975056.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKDYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(27284..27520)
                     /gene="dltC"
                     /locus_tag="O0V33_000372"
     CDS             complement(27284..27520)
                     /gene="dltC"
                     /locus_tag="O0V33_000372"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HCX8975057.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            complement(27538..28752)
                     /gene="dltB"
                     /locus_tag="O0V33_000373"
     CDS             complement(27538..28752)
                     /gene="dltB"
                     /locus_tag="O0V33_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HCX8975058.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            complement(28749..30206)
                     /gene="dltA"
                     /locus_tag="O0V33_000374"
     CDS             complement(28749..30206)
                     /gene="dltA"
                     /locus_tag="O0V33_000374"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCX8975059.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLAGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVSR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            complement(30222..30374)
                     /locus_tag="O0V33_000375"
     CDS             complement(30222..30374)
                     /locus_tag="O0V33_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCX8975060.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
     gene            complement(30422..30532)
                     /locus_tag="O0V33_000376"
     CDS             complement(30422..30532)
                     /locus_tag="O0V33_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975061.1"
                     /translation="MMTTNYYVESIKLKLNFIMNIDIMNCKKQILKRILY"
     gene            complement(30785..31744)
                     /locus_tag="O0V33_000377"
     CDS             complement(30785..31744)
                     /locus_tag="O0V33_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HCX8975062.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLATARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTVGIYGMGDIGKA
                     FARRLQGFNTKILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHNPVN"
     gene            complement(31777..32556)
                     /locus_tag="O0V33_000378"
     CDS             complement(31777..32556)
                     /locus_tag="O0V33_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCX8975063.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGFLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            complement(32556..32990)
                     /locus_tag="O0V33_000379"
     CDS             complement(32556..32990)
                     /locus_tag="O0V33_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HCX8975064.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVDIRKQFTYDYTALD
                     IKVIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ"
     gene            33088..33354
                     /locus_tag="O0V33_000380"
     CDS             33088..33354
                     /locus_tag="O0V33_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HCX8975065.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            complement(33433..33816)
                     /locus_tag="O0V33_000381"
     CDS             complement(33433..33816)
                     /locus_tag="O0V33_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCX8975066.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
     gene            complement(33945..34862)
                     /gene="lipA"
                     /locus_tag="O0V33_000382"
     CDS             complement(33945..34862)
                     /gene="lipA"
                     /locus_tag="O0V33_000382"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCX8975067.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
     gene            complement(34946..36265)
                     /locus_tag="O0V33_000383"
     CDS             complement(34946..36265)
                     /locus_tag="O0V33_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCX8975068.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKAYMAEQRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALDWIVTDPLDSIKEEIEH
                     QRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVKTSFEEEGKTLMSNPVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLSGVKLKEILAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY"
     gene            complement(36292..37119)
                     /locus_tag="O0V33_000384"
     CDS             complement(36292..37119)
                     /locus_tag="O0V33_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCX8975069.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQIAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKTFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            complement(37132..37980)
                     /locus_tag="O0V33_000385"
     CDS             complement(37132..37980)
                     /locus_tag="O0V33_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCX8975070.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            complement(38280..39347)
                     /locus_tag="O0V33_000386"
     CDS             complement(38280..39347)
                     /locus_tag="O0V33_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HCX8975071.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     EPVVKITEEQQFKCHIDTIIKKQVPVCCFTFGIPNESIIKRLKEANIKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVVTKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELTHMWSGQSPRLATT
                     HPANTIMSNIINQINQIMQYK"
     gene            complement(39361..40401)
                     /locus_tag="O0V33_000387"
     CDS             complement(39361..40401)
                     /locus_tag="O0V33_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCX8975072.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKEDQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
BASE COUNT        12881 a         7261 c         6148 g        14182 t
ORIGIN      
        1 ttcattaaaa gtttgctatc atgtgttcat acgaaatatt cagataacta caatgaggtg
       61 aattttatga aagagaaaat tgttttagca tattcaggag gactagatac aagtgtggcc
      121 gttcaatggc ttatcgacaa aggatacgac gttgtagctt gttgcctaga tgttggtgaa
      181 ggtaaagatt tagacatcgt ttataaaaaa gctttagata tgcgagctgt tgaatgtcat
      241 attattgatg caacaaaaga atttagtgat gagtatgtaa gttatgcaat caaaggaaat
      301 ttaatgtatg aaaatgcata tccattagtt tctgctttat caagaccatt aatcgctaaa
      361 aaattagtag agattgctga gaaaacaaat tcagtaggta ttgcacatgg ttgtactggt
      421 aaagggaatg accaagtacg tttcgaagtt gccattaaag cattaaaccc atcattgaaa
      481 gcattcgcac ctgtacgtga gtgggcatgg agtcgtgaag aagaaattga ttatgcaatt
      541 aaacataata tccctgtatc aattaaccat gattcacctt attctatcga tcaaaaccta
      601 tggggcagag cgaatgaatg tggtatttta gaagatcctt atgctgcacc accagaggat
      661 gcgttcgatc taacaaatgc tttagaagaa acaccagata ctgctgatga aatcatttta
      721 acgtttgata aaggcatccc agttcaaatt gatggcaaaa catatgaatt agacgattta
      781 attttaacgt tgaatgcatt agctggtaag catggtatcg gaagaattga ccatgtagaa
      841 aatagacttg taggtatcaa atcaagagaa atttatgaag cacctgctgc agaagttatt
      901 ttaaaagcac ataaagcatt agaaacgatt acgttaacga aagatgttgc acactttaaa
      961 ccaatcattg agaagcaatt tgctgaacaa ctatacaatg gactttggtt ctcaccttta
     1021 actgatagct tgaaattatt tattgatagt actcagcaat acgtaagtgg tgatgtcaga
     1081 attaaattat tcaaaggtaa tgctatcgtg aatggtagaa aatcacctta cacattatat
     1141 gatgaaaaat tagcaactta tacaaaagaa gatgcattta atcaagacgc tgctgttggc
     1201 tttatcgata tctatggttt acctactcaa gtaaatgcga tgttacatgg aggctatagc
     1261 aatgagcaat aaagcttggg gcggtagatt tgaagtacaa cctgaagagt gggttgacga
     1321 ctttaacgca tccattactt ttgatcaaac gctcatagat caagatatcg aaggcagcat
     1381 tgcgcatgca actatgcttg cgaatcaagg cattattagt caacaagaca gcgaacaaat
     1441 tatacaagga ctaaaatcta ttcaacatga ttatcatcaa gatcaaattc aatttagtgc
     1501 atcattagaa gatattcatt taaatattga acatgaatta attaaacgta ttggtgatgc
     1561 tggtggtaag ttgcatactg gacgcagtag aaacgatcaa gttgcaacag acatgcactt
     1621 gtacactaag aaacaagtgc aagatatcat cgcattaatt aagtcgttac aaagtgtaat
     1681 tgtagacatc gcttccaata atgttgatac aattatgcct ggttatactc atttacagcg
     1741 tgcacagcca atttcatttg cacatcatat tatgacttat ttttggatgt tacaacgaga
     1801 ccaacaacga tttgaagata gtttaaaacg aatcgatatt aatcctttag gtgcagcagc
     1861 cttaagtggt accacatacc ctatcgatag acacgagaca acagcattgt tgaactttgg
     1921 cagcctctat gagaatagcc tagatgctgt tagtgacaga gactatatta ttgaaacatt
     1981 gcataatatt tctttaacga tggttcactt atcacgtttt gcagaggaaa ttattttctg
     2041 gtccacagac gaagctaaat tcattacatt atcagatgca ttttctactg gctcatctat
     2101 tatgccacaa aagaaaaatc ctgatatggc agaattaatt agaggtaaag ttggtcgaac
     2161 gactggtcat ttaatgagca tgcttatgac tttaaaagga ttacctctag catataacaa
     2221 agatatgcag gaagataaag aaggtttatt cgatgctgtc catacaatta aaggttcttt
     2281 acgtattttc gaaggtatga ttcaaacgat gaccattaat aaagaacgac tcaatcaaac
     2341 tgttaaagaa gatttttcaa atgcaacgga actagcagat tatttagtaa ctaaaaatat
     2401 tccatttaga actgcacatg aaattgtagg aaaaatcgtc ttagaatgta tacaacaagg
     2461 tcattattta ttagatgttc ctttatcaac atatcaacaa catcattcta gtatcgatgc
     2521 cgatatttac gattatttgc agcctgaaaa ttgcttaaaa cgacgtcaaa gttacggttc
     2581 tacgggtcaa tcatcggtca aacaacaact tgatgttgct aaacaattac tatcacaata
     2641 aatacgttaa tctacctacc cacaatgtct atgaaaatta cattgtgggt attttaatgc
     2701 tctcttcgtc ttgttgaaca tcacattttt aagattccta aaatgtttga taattctttt
     2761 aaatttatat tacagaaatg ttataaattg taaaagaaat gtgtaaagcg ttttcacaag
     2821 caggtttttg tagtatttta aaattgttag actacaaata aagagatgaa aggataaaga
     2881 ctatgactaa ctcttcgaaa agcttcacta aatttatggc tgcttctgct gtttttacta
     2941 tgggattttt atcagtacct actgctggcg ctgaacaaac taatcatatt gcaaataaac
     3001 ctcaggctat tcaatggcat acaaatttaa cgaatgagcg attcactact atcgcacatc
     3061 gtggcgcaag tggctatgca cccgagcata cgtttcaagc atatgataag agtcataatg
     3121 agttaaaagc atcgtatatc gaaattgatt tacaacgtac caaagatggc catttagttg
     3181 ctatgcatga tgaaaatgtt aaccgtacaa caaatggaca cggtaaagtt gaggattata
     3241 cccttgatga attaaaacag ttagatgcag gaagttggtt taataaaaaa tatccaaaat
     3301 acgcaagagc aagttataaa aatgctaaag tacccacttt agatgaaatt ttagaacgtt
     3361 atggtccaaa tgcaaactat tatattgaaa caaagtcacc tgatgtatac ccaggaatgg
     3421 aagaacaatt attagcttca ttgaaaaagc atcacctttt aaataaaaat aaattaaaaa
     3481 atggacatgt aatgattcaa tcattttctg acgaaagttt aaagaaaatt catcgtcaaa
     3541 ataagcatgt gccattagta aaattagttg ataaaggtga actacaacaa tttaacgacc
     3601 aacgcttaaa agagatacgc tcttatgcga ttggattagg tcctgattat acagatttaa
     3661 ctgaacaaaa tacccatcat ttaaaagact taggatttat agtacatcct tatacagtga
     3721 atgaaaaagc tgatatgtta cgattaaata aatacggcgt tgatggtgtc tttacaaatt
     3781 tcgctgataa atataaagaa gtcattaaat agtattgtta aactagaaaa cataaataca
     3841 aaaatatagc tattactata aaaaacagca gtaagatatt tccaaattga aattatccta
     3901 ctgctgtctt tttgctcaac tatatatagt tgagcgttta attagaaatt ctctgtgttg
     3961 ggggcccacc ccaacttgca cattattgta agctggcttt ccaccagctt ctgtgttggg
     4021 gccccgccaa cttgcacatt attgtaagct gacttttcgc cagcttctgt gctggggccc
     4081 cggctatttt tgaaataagc ttgttacaag tgtattttta ttcagtcaac tactgctaaa
     4141 atataattaa cgagcttagg gctttgtttt ctgtcccaag ctcgttaaat cacatatgat
     4201 aattaattat gcccaaccac gatatctagc tgcttctgct gtacgtttaa tacctatgat
     4261 atatgctgca agtctcatat ctatttttcg attttgagac aattcgtaaa tcgtatcaaa
     4321 tgccgcttct aatttttcac gtagcttttc attaacttct tcttcagacc aataataacc
     4381 ttgattattt tgtacccatt cgaagtatga aaccgttaca ccaccagcac ttgctaatac
     4441 gtctggaact aataatatac cacgttcagt taaaatacgt gttgcctctg gtgttgtagg
     4501 tccattagca gcttcaacaa cgatgctagc tttaatatca tgtgcattgt cttctgtaat
     4561 ttggtttgaa atagccgctg gtactaaaat gtcacaatct aattcaaaca attctttatt
     4621 tgagattgtt tcttcaaata aatttgttac cgtaccaaaa ctatcacgac ggtctaataa
     4681 ataatctata tctaagccat ttggatcgtg taatgcaccg taagcatcag agatacctac
     4741 aatttttgca cctaaatcat ataagaattt agctaagaaa cttccggcat taccgaaacc
     4801 ttgaataaca accttggcac cttcaatttg catattacga cgttttgcag cttgttcaat
     4861 tgcaataact acacctagtg cagttgatct atcgcgtcca tgagaaccac ccaatacaat
     4921 tggtttacct gtgatgaaac ctggtgaatt aaatttatct aatgcactat attcatccat
     4981 catccaagcc ataatttgtg agtttgtaaa tacatctggt gctggaatat ctttgttcgg
     5041 acctacgaat tgtgaaattg ctcttacata tccgcgtgat aaacgttcaa cttcatgaat
     5101 gctcatttga cgtggatcac aaacgatacc acccttacca ccaccgtatg gtaagttaac
     5161 aatgccacat ttcaaagtca tccacattga taatgctttt acttcttctt catcaacatc
     5221 tgggtggaaa cgcacgcccc cttttgttgg tccaacagca tcattatgtt gcgcacggta
     5281 acctgtgaat gttttaacag tgccatcatc cattcgtaca gggatacgca cttgtaacat
     5341 tcttaaaggt tctttaatta aatcgtacat tccttcgtca aatcccaatt tatgcaatgc
     5401 ttctttaata attccttgag tagaagttac taaattattg ttctcagtca tgatcctttt
     5461 cgcctcttct ttacctaatg atttcgcttt caaacatatt gtaacataac gtattccttt
     5521 ttaaagccct tacaaactga ttgttacaac tttttgacat tattgaaata catgtcttat
     5581 tttttcaagt gcaaggtcca attcttcttt agtaataatt aatggtggtg caaaacgaat
     5641 gacagtatca tgcgtttctt tacataataa gccttcttct ttcaatgctt cacagtatgg
     5701 tctagcactt tcattaagtt ccacacctat aaacaaacca cgtccacgga cttctttaat
     5761 tgatggatga tcaatttgct ttaattgttc tttaaaataa tctcctaatt ctaaagagcg
     5821 acctggtaaa tcctcatcaa cgataacatc taatgcagca attgatgctg cacaagcaag
     5881 tggattacca ccaaatgttg aaccatgtga gccaggtgta aagacatcta atacttcttt
     5941 atctgctaat acaacagaaa ttgggaagac accaccacct agtgctttac ctaaaatata
     6001 gacatcaggt tttacattat cccaatccct agcaaataat ttacccgaac gacctaatcc
     6061 tgcttggatt tcgtcagcaa taaataagac attatgttca tcacataatt ctctaattgc
     6121 tttcaaatat ccttctggtg gtatatttat acccgcttca ccttgaattg gttctactaa
     6181 aactgctgca gtatttttat taattgcagc tttcaatgca tctacatcac caaaatcaac
     6241 ttttctaaac ccatctaata atggaccata accacgttgg tattctgctt ctgatgataa
     6301 tgaaactggc gccattgttc gaccatggaa gttaccatta aatgcaatga tttctgcttt
     6361 atttggctca attcctttaa catcgtatgc ccagcgtcgt gctgctttca aagctgtttc
     6421 tactgcttca gcacctgtat tcattggtaa agctttatct ttacctgcca gtttacaaat
     6481 tttttcgtac cattctccta agttatcact atgaaaagca cgtgaaacta gagtcacttt
     6541 atcagcttga tcttttaatg cttgaataat tttcggatgt ctatgacctt ggttaacagc
     6601 ggaatatgca gataacatat ccatatattt attgccttca ggatctttaa cccatacccc
     6661 ttcagcttct gaaatgacaa ttggcaatgg taaataatta tgtgctccgt aatgatttgt
     6721 taactcaata attttttcag atttagtcat catatctccc cttttcatca tttataacta
     6781 ttatacatga aacattatcc aaataattac attagttttc aaagcagata ctttttcacc
     6841 cgaaaagatg gattaatcac taagtttcat taaatttgtc tattttgaaa acccttacat
     6901 ttataatgac ataattactt aaatgattac aagcaaaaga attgataatt ttacacttaa
     6961 tcaaaagtat attttactaa gaatattttt atttataaat attgaaaacc actaacaaat
     7021 tgcatacaca atatcattag tggtaacagt caaacactta tttatcttta cgtggtaatg
     7081 gatagaaccc ttcattaaat tgatgccata aattaggagt taagtgatga ttatcttctc
     7141 tctctggatc aaagtaatta ataatttcat cttcccgtcc atctttaatt ttttcaacaa
     7201 attgataatc tagtagtagc tctctaccaa tggctactaa gtcaacacca acattttcaa
     7261 ctgcatctaa agcgtcgtca gctgtgaaaa ttgaaccaat accgataagt ggcatacgac
     7321 catttatcca tttgtgaatt aaaggcagtc tttcttgtcc agcgtattta ccttcacgtg
     7381 ccgttgcatg cgtatccatc attgaaacat gaatatagtc gattggcata tggctaattt
     7441 tattaacgag ttcctctgta atttccattg tgattcctgg agactccgct tcctctggag
     7501 ataatctgta tccaactata aagtctttat taccatacac ttctttcgat ttaagcactt
     7561 cttcaatcac agcgatcggg aatttatatt gatttgccca tacatcattt cttctattat
     7621 agtatggtga tacaaattga tgaattaagt aatgattcgc gccatgaatt tcaacaccat
     7681 caaaccctgc ttcaattgca cgtcgcgttg cttcaccaaa atccttgatt gcttgttcaa
     7741 tctcttcatt cgtcatttct ctagcactat gttcttgttt ctgaccgaaa ctttttaaag
     7801 aaattggact tggtgctacg acgtctccat caggtgttaa ttcaggcaat gcttgtgcac
     7861 cgccatgatg tatttgtacg agtgctttgg caccgttttt cttcattgct gtagctaatc
     7921 gttttagtcc ttcaatatca ctgtcatgcg cgattgacgg ctgtcctgga aatgcttttc
     7981 cgacatcact cacattactc gcagcattac ttgtaatacc aacatcttgt gaacgctttt
     8041 caatataagg aagttctata tctgaaatag taccatcatc atttgaagaa atatgtgtta
     8101 aaggggctaa cacaaatcga tttctcaact ctactccatt tggtaatact actttatcaa
     8161 acaatggttc gtatttactt ttcattactt aattcctcct taaaattatt gagttgacta
     8221 taacatgtaa ttttttcatt ctccaattat ctgtttatat agttgcaatt cgtttaatac
     8281 tttaagttgt agttctcatt tctaaaataa attaaaaaaa caaatccaca tacatcattg
     8341 caaatcaacg attctatcta ataaagataa tggatatgtc accttaaacc atttgtacgt
     8401 gtaaatagaa tcaatcatat tacgttaacg cagacaaaaa gagattagta tctaatataa
     8461 tttaatagat actaatctct tttatagtct aagaaacttt catttcagac cctttgtatg
     8521 attttcggta cgatttatct gttcctttag tcgtttcgaa ttgctcgctt tgactgtttt
     8581 atccagattg gtaagcgttc tttaagtgtt tgaaacccat atttttctgt ttctctgatt
     8641 tcatctaata ctgattgttt ttccttctta cgctcataat ttttgaagta atcattatcc
     8701 tttaatgata gattgagctt tccttcatca tctatagaca aaattttagc tttaacaatt
     8761 tggccttctg atagaaattt cttcaaatta tgaacgtagt catccataat ttctgatata
     8821 tgaatcagtc cttcagtatg attaggggtc tcaacaaacg caccgtatgg ttgaatacca
     8881 gttacacgca ccttgatatg ttggccaatt ttgtagttat tcaacttaat aaccccttat
     8941 ttcgtttact taacactatt atcatagcac aaatcgaaat ggtggactaa ttaaaacatc
     9001 aaatcatacg aatattattt tataaatgaa taagaatact acaaacttgg atttacaaat
     9061 aaaatcagca acaaattcct atctaaatca atatgatatc cagctacttt gttttcaatt
     9121 tattaactat ttgaatagca attctctgta aaacataaaa agcaacctca actattatct
     9181 ctagaataca catctattta aaaactgtgc tttcttgata aatatattga gattgctcat
     9241 catattagtt tatttgtttt cgttggaata tatactaatc cattcataaa ttagctttat
     9301 ccgagtttaa aatgttggca gttaattatg tttagatatt attcttcaac atcaatagat
     9361 tcaattacaa catcatgaag tggtttgtct tgtggtccca cttttgtatt tgcaatatct
     9421 tctaaagtag tttcaccatc aatgatttga ccgaatactg tatgtttttg atctaaccat
     9481 ggtgtaccac ccttttcgcc atatgcatca acgattggtt gaggccagcc accatctgca
     9541 agttgactta acatattttg aggtacttct ttcatttgaa caatgaaaaa ttgtgaacca
     9601 tttgtattag gtcctgagtt agccattgat aatgcgccat ataagttaaa tgcatttaac
     9661 gaaaattcat cttcaaaagc accgccataa atactttcgc cacccatacc agtagctgtt
     9721 ggatcgccac cttgaatcat gaagtcatta atgacacggt ggaatgttat tccatcataa
     9781 taaccatttt ttgcatgtgt cacaaaattt tcaactgttt ttggtgcaat atttggaaat
     9841 aatttgaatg tcatgtcacc tttatttgtg tgcataacca ctttaatttc accttgttgt
     9901 acttctttgt ttaactgtgg atagttagcc atttatattc tccattcatg ttatgataca
     9961 tatatattaa catactttta agggaggaaa atagcatgaa gctatatcga ttttcacaca
    10021 aaacaggtag ctatggtgtc acgattgttg aagaaaatgg agacgatgtc cttgttaaag
    10081 ttgaacaagt tatcaaacac ccgaaacaag gtgatttaca taaccctact gaaactgaag
    10141 gtgtattttt ccatgaacgc aaagcattaa gtcattttga aaaacgctat acgaaacgtt
    10201 cacaattacg cgacttcaat gttgatgtat tgcgctatga agattcactt caacaagcaa
    10261 ttacagattt agaaactaaa cttaaagctc agcaaacaaa acatgctgaa atgtctttag
    10321 ctagtttagc acgcttaaaa gaagattatt cacttcaata caaagaaaat tttaaataaa
    10381 aaatgacgtg tttcttctta tttaaatact tctgaaaata aagcacaaca aggagtgtgt
    10441 acgtttttca gaactatatc attcatgtat aatgaataac ataaatattt tttaaaggag
    10501 gacaacatag tgagtttgtt acatattgcg gtgatattac cgttaatatt cgcactcatt
    10561 attcctatcc tgtatagatt ttttaaacgt atacatttag gatggttcgt attacctgtc
    10621 cctatcgtca tatttattta tatgcttaca ttaattaaaa cgacgatgtc aggtaatact
    10681 gtaatgaaaa ccttaaattg gatgcctcat tttggtatga actttgattt atatcttgat
    10741 ggcttaggtt tactctttag tttgcttatt tcaggaatcg gcagtttagt agtattatat
    10801 tcaatcggct acttaagtaa gtcagaacaa ctagggaatt tttattgtta tttattacta
    10861 tttatgggtg caatgttagg tgtcgtactt tcagataatg ttattattct atatttattc
    10921 tgggagctaa cgtcattttc tagtttttta cttatttcat tctggcgcga acgacaagca
    10981 tctatatatg gtgctcaaaa atcgcttata atcacagtat ttggtggtct atcattatta
    11041 ggtggtatca tcttactcgc tataccaaca caatcattta gcattcagta catgattcaa
    11101 catgcttctg aaattcaaaa tagcccattc ttcatttttg cgatgatttt aatcatgatt
    11161 ggtgctttta caaaatctgc tcagtttcca ttttatattt ggctaccaga tgcaatggaa
    11221 gcacctacac cagtcagtgc ttatcttcat tcagcaacaa tggttaaagc agggttatat
    11281 ttaatcgctc gaatgacacc aatcttcgca gcatcgcaag gttggatatg gactgtcaca
    11341 cttgttggtt taattacgtt attctgggct tcgctaaacg caacaaaaca acaagacttg
    11401 aaaggtatac tggcattttc aacagtatcc caattaggta tgattatggc gatgcttgga
    11461 atcggtgcca ttagttatca ctatcaaggc gatgatagca aaatatatgc tgctgcattc
    11521 acggctgcaa tattccattt aatcaaccat gcaacattta aaggtgccct atttatgatt
    11581 actggtgcag tggatcattc tactggtaca cgtgatgtta agaaattagg tggcttacta
    11641 acaattatgc ctatttcatt tacaatcact gttattacag cattaagtat ggctggtgta
    11701 ccaccgttca atggtttctt atcaaaagaa tcatttttag aaacaacatt tacagcaagc
    11761 aaagcaaatt tattcagtgt tgatacatta ggctacctat tcccaatcat tggtatagtg
    11821 ggcagtgtct tcacatttgt atattcaatt aaatttataa tgcatatatt tttcggacaa
    11881 tataaacctg aacaattacc taaaaaagca cacgaagtat caatattaat gcttttatca
    11941 ccagctattt tagctacatt agtaattgta tttggtttat tccctggcat tttaactaat
    12001 tcgattattg aaccagctac atcttcgatt aatcatacgg taattgatga tgttgaattc
    12061 catatgttcc atggattaac gcctgccttt ttatcaacat tagtgattta tattttaggt
    12121 atcttactaa ttgtgacatt tagttattgg gtgaaattgt tgcaacgaca acctggtaaa
    12181 ctaacattca attattggta caacagaagt gcaaatgtta ttccaaacta ctctgaaaag
    12241 atgacgaata gctatgttac cgattattca cgtaataatt tagttatcat ttttggtgca
    12301 ttaattttat taacatttgt cactgtattc tcagtacctt tcaacattaa ttttaaagac
    12361 gttagtccga ttagaatttt cgaagtttgt atcgtaattt tattattaag tgctgccttt
    12421 ttaattcttt tcgctaaatc aagattattt agcatcatta tgttaagtgc agttggatat
    12481 gctgtgtctg tattgtttat attctttaaa gcacccgact tagcattgac acagtttgtc
    12541 gttgaatcta tttcaacagc attgttctta ttatgcttct atcacttacc aaatttaaat
    12601 cgttataatg aaaagcgctc attccagctt actaatgctt taattgctgg tggtgttggt
    12661 ttgtccgtca ttattatcgg tttaattgca tacggaaata gacattttga atcgatttct
    12721 aagttttatc aagagcacgt ttatgattta gctcacggta aaaacatggt aaacgttata
    12781 ctagttgatt tccgtggtat ggatacttta ttcgagtcat ctgtgcttgg tatcgcagga
    12841 ttagctgtat atacaatgat taaattacgt aaaaagcgtc aaacgcaagg taacgaggtg
    12901 aaaaatcatg aatagacaac aaaatgatct aattttgcaa tttgctgcag ttattatctt
    12961 tttcatggta atggtctttg gtttttcact tttcttagca ggacattata cacctggtgg
    13021 cggtttcgtc ggcggattat tattcgcaag ttcacttgtt atcattacga ttgcatttga
    13081 tatagaaaca atgcgtaaaa ttttcccatt agattttaaa atcttgatag gtatcggatt
    13141 agttttttgt attgcgacgc ctattgcaag ttggttttta ggcaaaaatt tctttacgca
    13201 tgtcacattt gatataccgt tatttatatt agaacctgtt catatgacaa cagcagtatt
    13261 ctttgatttc ggtgttttat gtgcagtagt tggtactgtc atgacaatta ttatttcgat
    13321 tggagagaac gaatagtgga aattattatg atttttgtta gtggtattct cacagcaatt
    13381 agtgtctatc tcgttttgtc taaaagtctg atacgaattg ttatgggaac tacgctatta
    13441 acacatgcag caaatttatt tttaataact atgggcggac ttaaacatgg tactgttcca
    13501 atttatgaag cgaacgtaaa aagctatgtt gatcctatcc cgcaagcact tattttaaca
    13561 gcaatcgtta tcgcctttgc gacaacagcc tttttcttag tattagcatt tagaacatat
    13621 aaagaattag gcacagataa cgttgagagt atgaaaggag ttccagaaga tgattgaaag
    13681 taatatgctt gtgttaacgc tcgtcattcc agtaattaca gccatattac ttgtctttat
    13741 tggaaaacgt cctattataa agcgttatgt cgcactaggc ggtacattat taaccttggt
    13801 cgcggcaatt atcaatttag caaatgttgt aaaacatgga ccattacgag ttgaactagg
    13861 ttcatggaaa gcgccttaca gtattgtctt tgttttagac atattcagtg ccctactaat
    13921 tattactagt attattatta cagctattgt gattttatat tcataccaaa caattggtat
    13981 tgaacgtgaa agatattatt attatttttc agtactattt atgttaatag gtatcattgg
    14041 tgcattcaca actggtgaca ttttcaacct attcgtattt ttcgaagtat ttttaatgtc
    14101 atcttatttc ttattagtaa ttggttctac aaaaattcaa ctacaagaaa caataaaata
    14161 tgttcttgtt aacgttgtat cgtcatcctt cttcgtaatg ggtgtagcga ttttgtattc
    14221 agtagtagga acattgaatc tagcagatat aagcaataaa ttagctaacc tttcagcgca
    14281 tgacagtggc ttagtcaata tcgttttcat actatttatc tttgtttttg ctacaaaagc
    14341 tggcgtattc ccaatgtttg tttggttacc tagtgcatac tatgcaccac caattccaat
    14401 cattgcattc tttggtgctt tgttaacaaa agttggtgtt tatgcaatca ctagaacatt
    14461 gagcctattc tttagtgata atgtaagctt ttcacattat gtcattttat tcttagcact
    14521 attaacgatc atctttggtt gtgtcggtgc tgttgcatat gccaacatta aaaaaatcat
    14581 cctatacaat gtcatgattg ctgtaggtgt gatcttagtc ggcgttgcaa tgatgacaga
    14641 gtcaggtatg attggtgcta tttactatac attgcacgac atgttagtaa aacttgcatt
    14701 attcttgctt atcggaatta tgattaaaat tacaggcact gctgatttaa gacaatttgg
    14761 tggcttaatt aaacgatatc ctgtacttgg ttggtcattc tttatcgcag cattaagttt
    14821 agctggcatt cccccactca gtggctttta tggaaaattt ttcattgtcc aatcaacatt
    14881 tgaaagagga ttttatttaa gtggtgtcat cgtcttatta tcaagtttag tcgtattata
    14941 ttctgtcatt cgcatattcc tacaaggctt tttcggtcaa ccaaaaggat atgatcttaa
    15001 taacaaagtc gacgtcaaat atttaactac gattgcaatc gttgcagttg tgataactgt
    15061 tttatatgga ttgtcagcag attacttata tccaatggta aaagctggtg cagaaacatt
    15121 ctacaatcca tcaacctacg ttaaagctgt attaggaggt aaataacatg gctgtacaat
    15181 tagttttaaa ctttattatt gcagtgtttt ggttgtttgt gacaaatagt tacacaacaa
    15241 ataactttgt gttaggtttc atctttggcc tagttcttgt ttacctctta cacagagtgt
    15301 taccaggtag attttatgtc atcacgctat atcgtattat taaattagtt attattttct
    15361 taatcgaact gattaaagct aattttgatg ttttgaaaat tattattaaa ccttcgatta
    15421 aaaatgaacc aggattcttt gtttatcaca cagatttaaa aaaagattgg caaattgtct
    15481 tgttgtctaa tttaattaca ttaacacctg gtactgtagt gcttggtgta agtgatgatc
    15541 gtacaaaaat ttatattcat gccatagatt tttcaactaa agagcaagaa gtagaaagta
    15601 ttaaaacatc attagagaag attgtaagag aggtgggcga aatataatga atcataatgt
    15661 tattatcgtt attgcattaa tcatagttgt catttctatg ttagctatgc tcattcgcgt
    15721 tgtgctaggc ccatcacttg ccgatcgtgt tgtcgcatta gatgcgattg gtcttcaatt
    15781 aatggcagtt atagcattat tcagtatttt attaaatatt aaatacatga ttgtcgttat
    15841 tatgatgatt ggtatattag cctttttagg tactgcagta ttctctaaat ttatggacaa
    15901 aggtaaggtg attgaacatg atcaaaatca tactgattag tcttgcactt atctttgtta
    15961 tcatcggtgc tttaattagc gccctagcag ccataggatt attgagactt gaagatgtat
    16021 attcacgtgc acatgctgcc ggaaaagcat caacattagg tgcaatgtca ttactatttg
    16081 gtacgttttt atattttatt gctactcaag gttttgtaaa tatgcaatta atcgttgcga
    16141 ttatctttgt attaattaca ggtcctcttt caagtcatat gattatgaaa gcagcatata
    16201 atattaaaac gccttatact aaaaagacta aagtcgatga aatttctgaa gacttaaaag
    16261 acacaaaatt atagattata taacaaagtc atgaatataa atcaacaaca aacagcagta
    16321 agatgattcc aaattaggaa tgattttact gctgttttct tttgacattg ttacctcttt
    16381 ttcaatgatt ttagtttagt acatacttct ttgactacac cttcgcccta tctaggtata
    16441 tctctttaat ttaattgcct ttcatgtcgt tatgtattat gataataatg attataaatc
    16501 gtaacgatta cgctttaaaa agagagaggt tttattatgc attggacaat tatcggcggt
    16561 ggcatacagg gaactgcaat cgcacaaaaa ctattatcaa gcggattaac aacagaccga
    16621 ttaacaatca ttgacccaca tgaaactttt tgccaaaggt ttaactcata tacaaatcga
    16681 atagaaatgc cttatttaag atcaccgatt gtacatcacg tacatccaca accgttccat
    16741 ctaaaacaat tcgctaaaca gcaccaatat acaaatgctt tttatggtcc ttatcagcga
    16801 cctgaattga caatgtttat ggatcatatt gcacatgctt ctaaacaata tcaattagag
    16861 gattgcttgg ttcaaggttt agttcaaact ttagataaac aagaagacaa atggcatatc
    16921 aagttagaag atggacaaat tatcactaca gattgcgtcg ttattgcaat aggcagtaca
    16981 aatattccgt ttatgcctga cattttaaaa gacaaacaga atgtaaatca tatcttcgag
    17041 aaagaacatg atcaagtagt atatgataag accgatcata tcgttggtag tggtattact
    17101 gctgcacatc ttgcacttaa attgttaaat catgatgacg ataaaaagat tcatttatgg
    17161 ctaaataaag atattgaaat acatgacttt gatgctgatc ctggttggtt aggtccgaaa
    17221 aatatgtctt catttttaag tactaaaagt atgcctgaaa gaaatgccat tgtacaacgt
    17281 gaacgtcata aaggatcaat gcctcacgaa ctgtacttac gccttaaaaa acatattaaa
    17341 aatggtcgta taaatgtgca taaaacacct atcactcaaa ttagtgatgg tttaattaac
    17401 actgaaaatg attctgttcc atatcaacag attatggttg caactggttt tgaacaagat
    17461 tttatgtcac aaccacttat taagcaatta atacaaaatt atgatgcacc tatcaacgaa
    17521 tgtaattacc ctgttatttc cgaaaaatta gaatggatac caaatctata tgtcgcagga
    17581 tgctttgcag acttagaatt aggaccattt ggtagaaatg ttatgggtgg ccgtaaagct
    17641 gccgaacgca ttgaacaagc atttctaaaa ttacaacaat atagcgcata aaaaaactgc
    17701 tagccgtagt tatgaatatc tcataacttt tcagctagca gttcttttat tttaaaggtt
    17761 taatagcaac tgtaccacgc ataactgtaa ttaattgttc tttgtcattc ttaattttta
    17821 tatcccatac atgtgttgac ttaccttgat gaataatttc agctgtcgca ataacacgac
    17881 catctttagc agaatgaata tgattggcat tcatctctaa acctaatgga ataaatttgg
    17941 ttgtatcaat tagattagca gatcctaatg aacatgctgt ttcacctaaa gcaatcgaag
    18001 caccgccatg taaatatcca aatggttgtt ttactttatc agtaacaggc atagaaataa
    18061 caactaaacc atcttcctgg tgatctattg acatctcaaa tgtctctaat aaatgagtca
    18121 tattttcacc tctttatgct tatagtacca tagcagcaat agtaccgaaa atcattaaag
    18181 gaatattata aaacaagaag tttggtacac atgtatcgcg tatatgatca tgttggccat
    18241 caacatttaa tcccgcagtt ggtcctaatg ttgaatcact tgcaggtgaa cctgagtcac
    18301 ctaatgcact cgctgttcca atcaatgcga ttaatgccat tgtatctagt ccaattgacg
    18361 ctccaaaagg aatgaataat gatgcgataa taggaattgt tgcaaatgat gatccaatac
    18421 ctaaagtgac aattaaacct atcacataca tcatgataat gctaaataat ttattatcac
    18481 cagtaatact tgttaaagtt ttaactaatt catctatatc accagtagca ttcattacac
    18541 cagcaaatcc atttgctgtt aaaataacta caccaatata agccataatt ttaatacctt
    18601 caacaaactt agcatctaat tcataccaat tatatgcacg tgaaataaag aatacgagta
    18661 ccccggccag tgcaccaaaa atcattgaat ctgtaaacgt ttgtactaaa aatgtagcta
    18721 gtattgccac aattgttact attaagatat atggttttaa ctctgtaaca ttgtcactat
    18781 ctgaaatttt acgtgtttca tattcacgtg gtttacgata tacatataaa ccgataagta
    18841 agccaacaat ataccccatt gaaggaataa gcattgcttt ccaaatcata ttaaactcaa
    18901 ttgggtgatt cgcctttgca aagccacttt gaataatttg ctggaaaatt tgaccgaatc
    18961 catatggtaa taacacatac gggaaacata aaccaaaacc gataatcaaa ccgattaaac
    19021 gtctatctat ttttaagtca ttaaacagac ttaataatgg tgggatgaca attggaatga
    19081 atgcaatatg tacaggaatt aagttttgac tcatgatact catagctaat aatgcaatga
    19141 ttattgtcac tttcacttta actcttgacc atcgactatt ttcagcgtga attgcattta
    19201 ttatttttcc tactaaatag tctgtgatac cactgtatga aattaatgct gcaaatccac
    19261 ctaataaggc atagcttaat gctacctcag caccatcgac tatatttttc ccaaatacat
    19321 ttataacttt ttcaatgctc atgcctgaaa ttaagccacc tactagcgca ctgataaata
    19381 agcttataac tacgtttaat cgacataaac atagcacaat cattaatatt actgctatta
    19441 ctactgcatt tatcatatga caaccaccct ttatcactct atcgaactaa agcgtttata
    19501 gactacatag tatcagtagc tttaacacct gtcaatagca aactatacca ataatcttta
    19561 ttttttagtt aattgtcttt cttaaaataa ttttcgcttt cattaaatta aacaatttta
    19621 caagtctgga acaccaatca aaatcctaag ttctataatg ttatattagt agtcgttgac
    19681 tgaatgaaca tatgcttata ataagttttt gcaatgctaa tcaagttgat ttatgctcac
    19741 aaggatatgc gatttatatt ttcttacaac aatgaaaatg cctgatacaa tgcgatcctt
    19801 tatactttca agagttaaat tagaaatgta taacaagaca caagttgtat caggcaaata
    19861 attaaattat tgttgtttta accattgtac tatcgtcgga atcataaatc ctgttggccc
    19921 ttttggacca ttatatgaag ctttattagt cgttgctgga cctgcaatat cgaaatgaat
    19981 gtgaggtgtt tgaccactaa aatgtgttac aaaacttgcc gcaaatagcg ctttaccttg
    20041 tccattcgta tggttaacta aatcagcgat atcactgtgt ttaatacttg cacgttcggt
    20101 tgcagtaatc ggtaattcaa ataccatttc atcgacttta gaacttattt gtaatatgtc
    20161 gtttaatatc actttactat tcgattcaaa tgcagcagct ttatcatcgc caagtgcaac
    20221 gatggctgca cccgttaatg tagcaaagtc cataatcaca ctaggctgat attgatttgc
    20281 ataatacaca gcatctgcaa ggactaatct accttcagcg tctgtattca ttacttctac
    20341 agtttcacca cttaacgctg taaatacatc atctggcttc attgatgctt catttatcat
    20401 attttcagca caagcaagca ctccgacaat atttacaggc agttgtaaac gactagccgc
    20461 ttcaatgata ccaacgacat tcgcagcgcc acacatgtca tatttcattg tagccatgcc
    20521 attcttcgtt ttaatactat aaccaccaga atcatacgtt atacctttac caactaaggc
    20581 aattggtgct tcatctttgt ctttgccatt atatgtgatg gttactaatc tcggtttatg
    20641 cttactacct ttacctaccg cttgtaaaag tccaaatcct tcagaaacta aagtgtcata
    20701 atctttaacc tctactttga cctttgtatt tttaaaatga ttaacaatat cttctgcaaa
    20761 tgtttgtggt gttaatacat tcggtggcat attactaaag tctcttgcca aattaatgga
    20821 ctggccaata ctgatacctt catgaataaa atctaattca attaatgatt cactaataag
    20881 gtttaaattt gtcttaaacg gtgccttctt acttgattta taatgatcga attcatatgt
    20941 tgcacgctca ctttgaatac cgcatgccat taatacatct gataactgat catattttga
    21001 aataaatgaa tccataagta aatacgtatc ttcaatgtgt tctgacttta tgtattggaa
    21061 aagatgtccc catattttca acatatcttg ataactacgt gtttttaagt ttcctaaacc
    21121 aactgtaatt aaacgatatg tttgatcttg tacatcaaat gcagttgtat aaatcttccc
    21181 aactttacta ccaataatat gttgatgttt tagtctttca agtgattctg taatatcgat
    21241 atgattaaaa ctaatgcgct ctaactgatt taaatgttct ggtataccaa taatcaatgt
    21301 atttatttcg ttgcttattg tgttatttaa tttaaaattc ataatgtacc tccttagatt
    21361 tgatatgtta ctcacttcaa attgtacaac aataaagccc ttcagtgaca ctgaaggact
    21421 ttataaatga ataatttaat tttatgtgtt tagctgtaac ttaatttaaa gcctagaatt
    21481 tacctttttt gaaagctaaa ccgataccac cgattttgaa taccgcacgt gtatcaatca
    21541 cttttttcat gaatgctgct tttttaccag cgataggttt accaaatacc atacctacac
    21601 cgtcatgtga acctaaagaa caaacagttc cacgatcaac gtattcgaat tcttcagttg
    21661 attcaccgtt taagatgcgt ttaatgtttt tagcaacact ttcaccttgt tgcattgcaa
    21721 tttgtgctgt agttggtaat ggacgctctt ctccagctgg gataaacgct gaacagtcac
    21781 caataacaaa aatgttgtcg taaccattga ttgttaaatc ttgctttgta acgatacgtc
    21841 cacgtttaac gccttcaaat gattcttcca ttaatttact accacgtaca ccagcagccc
    21901 atactgaagt acctgcattt aattgttgtt tttcaccatc tacttcaact acgaaacctt
    21961 tttcgttaca agcaacgatt ggtgtagcaa ttttaaattc aacaccgcgg tcttctaagt
    22021 agctaactgc gtggttaact aattcttctg agaacattgg taacatttta ggtgctgctt
    22081 caacacaagt gattttaact ttattttgat ccacaccata tttgctacat aattcaggaa
    22141 ttctgtctgt taattcacct aagaattcaa caccagtgaa tccagcacca ccaactaaga
    22201 tagataaatc gttatcatct ttttcttttg atgctgcata gttagcaaat ttgtcttcga
    22261 tatgacgtga taattcacgt gctgtgataa cattttcaat ttggaaagca tgatctttca
    22321 taccttcgat gccgaatgtt tcactaacga aacctaatgc tactactaaa atatcaaagt
    22381 cataaatacc ttgatttgtt tctacctttt tagcatcacg gtcaattttt gttacttctg
    22441 cttgaacaaa gttcactttg tctttcttca agacactttc cacaggatat aatacatctt
    22501 catagtttag tgtacctgct gatgcttcat gtaaccatgt tgcttcatag tgatattcat
    22561 ttttattaat aagcgtaatt tctgcttctt ctgttgatat cgctttttgc aatttagtta
    22621 cagtttgtaa acctgcataa ccagcaccaa gtacaagtac ttttttacga tcttgagcca
    22681 tttaatttca cctaagcttt catatttttt taaccaaatg ctgataatta ctagtaacaa
    22741 ctggtcatta ctacttttac cacaatgtat actagtttat catcattttg ttatttaagt
    22801 cttttaaatt atgtaaaaac ctgtgatgtt cacaaaaaag acatttttaa tccaatacta
    22861 attctatagc ttttatgtta tattttcaag caagattcag tcagattgtg aaaaaattaa
    22921 ttaatttttt cgtaacctga tttcgattaa aaatgtatcc gttatcacga tcatttaaaa
    22981 tactattaat agtcagattc ttaattttta atgagatttt taataaaaca aaatatcttt
    23041 acgcaaaaat aggacataat caaatatgtc cctaatcgta attgatatca atttgtatcc
    23101 ccaaaattgg gttaatcatt aaaaagttat tttattagca gttttcagga ttacctgcaa
    23161 cttttgcagt tctaaatgaa ctaccacagc cacatgatgc aattgcatta ggattgtcga
    23221 tttggaaacc gccacccatt aatgattgct taaaatcaat agtcgtacca tttaatacgg
    23281 gtgcatcttt tttgtctact aaaactttta atccaaagta ttctaagact tcatcatttt
    23341 caccaggcgc ttcttctgca cccattccgt atgttaaacc agtgcacccg ccaccattca
    23401 ctttaatttt taaatagcca tctggcattt catttgcttt aagcatatct tttacttcgt
    23461 aggcagctgc ttctgttaat ataactgttg gcatgataac tcctccttaa aaaatccaag
    23521 tttcttttat atgtgcatat atattttgta ataattcttc cggcgaatca ccttcaacaa
    23581 tatcaccatt tactaaagca tacaacccgg ctgaacatat accacaatgt gtcaggcaac
    23641 catactctaa cacatcgaca tctgggtcat tttccagttg attaaaaaca taatctccac
    23701 cttttgccat gttagagaga caaaattcta cgatcggatt catacttcac cttcttattt
    23761 catttgttac aatattatag cattttaaaa ctggtatttt aacatgatgt gctcaattag
    23821 caacaactga tgtttcttat cccagttatg taatagtgcc ttagttagga caaatattta
    23881 tttgtagtca ttttcaaaaa aatatataat aagcatataa atacatattt acttttttag
    23941 gggttattga tgaatcaaag acacttgcat tttataactg ttcattaaca aatatatcta
    24001 tgcactttga tcttttcaaa attaaagtaa atattaacat ataaaagggg tacttcatta
    24061 tgaaaaactt agttttgtta ggcggcggat atggtaatat gcgtatcatg tcacgcattt
    24121 taactacatc tttaccacaa gattatacag tcacattagt tgatcgtatg ccatttcatg
    24181 gattgaaacc agaattttat gctttagctg cgggcacgaa atcagataaa gatgttcgca
    24241 tgaaattccc taatcatcca caagtgaata cggtttatgg tgaaattaac gacatagatt
    24301 tagatgctca aattgtctca gtcggtaatt ctaaaattga ttatgatgag ctaattattg
    24361 gtttaggatg tgaagataaa tatcataacg ttccaggagc cgaagaatat acacatagta
    24421 ttcaaacact ctcaaaggct cgggatactt tccatagtat tagtgaacta ccagaaggtg
    24481 ctaaagttgg tatcgttggt gctggattaa gcggcataga acttgccagc gaattaagag
    24541 aaagtagatc agacttggaa atatatcttt atgaccgtgg gccacgaatt ttaagaaatt
    24601 ttccagaaaa attaagtaag tatgttgcga aatggttcgc caaaaataat gttaccgttg
    24661 ttccaaattc aaatattaat aaagttgaac ctggtaaaat atataactgt gatgaaccta
    24721 aagatattga tttagttgta tggacagcag gaattcaacc tgttgaagtt gttcgtaact
    24781 tgccgattga tataaatagt aatggacgtg tgatagttaa ccagtatcat caagtaccaa
    24841 catatcgtaa cgtctatgta gttggtgatt gtgctgattt accacatgcg ccaagtgctc
    24901 agttagcaga agttcaaggt gatcaaattg ccgatgtgct taaaaagcaa tggctaaatg
    24961 aaccattacc tgacaaaatg ccggaactaa aggtacaagg tatcgttggg tcattaggag
    25021 ataaacaagg ttttgcatat atcatggatc gcactgtaac aggacgactc gcttctattt
    25081 tgaaatccgg cgtactttgg ttatataaat atcataatgg ttaaaaatta aaaagcaatg
    25141 tcagttcaca ctattactga cattgctttt tattatgtgt atattattca ttcacaagct
    25201 tttggtctat aaatcgagtg atttgctttg tttgtatata accatctgct acatattcat
    25261 cgttcattgt aattaatgga taaaatagtt catcttgttc tattctttca ataaattgta
    25321 aatcatgatc tgttaagttg tcattatctt ttgtaatatc tatatacgta tatttaaatg
    25381 atatatttgg gtattttctt tttaatagcg gctgtagcca gtcataaata tctttcgatg
    25441 ttggcgcatt aacgcaactt gcacatataa catctgcccc atatactatc acactcacgt
    25501 gctccatata tttatccccc attggtttga tagattttta ttacactatc tattataata
    25561 tactaataaa gattatcaaa ttcatctctc gaaaggagac ttgcctgatg cctactgaag
    25621 atacaacgat gtttgatcaa gtagcagaag ttattgaacg tcttcgccca tttttattac
    25681 gtgatggtgg cgactgctca ttgattgacg tggaagacgg tattgttaaa ttacaattac
    25741 atggtgcatg tggtacatgc ccaagttcta caatcactct taaagctggt attgagcgtg
    25801 cattacacga agaagtgcct ggtgtaatag aagtagaaca agtattctaa tataaagata
    25861 ttaagttaac agaacatatt atgaataaca acaaagacag gaatactgaa ttatttataa
    25921 tcagttatta cctgtctttt tcgctgtcaa attggactga tgggaaagtt attttatact
    25981 atttatctga ttaattattt tataatagcc acatatccaa gcgcatatta ctattctatt
    26041 ctatgaaatt taaaatataa aaatagcaca taccctcaat caaaatcgtt gagttatgtg
    26101 ctatttgtat tttaattttt aggtttatct acttcaggtt gtggttcacc tttcatatgt
    26161 ttcgcaattt gctcatccat ataaacccaa cctttccaac cgatgtgtac ggcatcactg
    26221 attacatatt tttcataatc tttatcagtc atatcgtaaa ttttaccacc attatctaca
    26281 acagtagaat ggattttttt ataaactgct tgacgacgtt ctttatcgat accaatgtgg
    26341 tcataccata caccgtttga tggaatactt acatattgaa catctgcacc agcagcacgc
    26401 attgttttta caagtaattc taaatcttgg aattctggag aattaacatt gaattcatag
    26461 tcacgtctaa ctttacgctt actttcttga attaatttcc agtattgatc tctaatacca
    26521 aatttattcg atgtagtatc agctttacca atttcaactg ctttttgttt catctcatcc
    26581 caagaagcgt ctggttttgt agcaggttta acatgttcta atggagattt atccattgca
    26641 aacaatgatt taatcgcttc tattttaatc aattgattct ctttaaaacc agaaatataa
    26701 ctatctttag tttctttagg gtttttagca taagatttca agtattcttt attgtgtaca
    26761 tgtggaaact gtaataaacg ttgtgcataa cgacgtttta attcagtaga catgtttttc
    26821 tgctggaaca tttgattaat ttgagtttga gacatacgag catcaaagtt ttgattcgtt
    26881 aaaccatgat ttgtaaacca ttgtggtgaa ataataaatg ttaatttctt accttttaat
    26941 ttatcatact gtgatgcaag ttcaactgcg ttaattaagt ctgtagaacc accagcacct
    27001 aataagaatg cttttttgtt ggcgttatgc ttatttaatg caattgcagg attaaatggg
    27061 tcatctttac ctaattcact agagccgtat ataggataat acttgtttga ttcgtataac
    27121 ttatcttgaa tgagtgtgcc ttttagtact tgatctgtca atgaagttct attatcttct
    27181 acagtctttt catttactaa tcctgtaaac caactagcag gtaataatag aaagacaatg
    27241 aataccgctc cactaattaa aatgggtaaa aaaggtttta atttcatcgt aactcttcta
    27301 atgcttcaac gattttattt ggtgttgccc actcatctct atcaaagtcc ataatagata
    27361 cttcgatatc aagtttattt tgaatctcta ataataatcc aactgtttgg aaagaatcaa
    27421 taataccttc ttcaaaaatt tctacgtctg gattttcttt tacaatatca ttttctgcta
    27481 cttctgctaa taaatttaat acttgttctc taaattccat aattaaattc tcctttatta
    27541 tataagttta cctgagaaga ttaaaaagcc aaatgttaca aagtggaatg taatcacaat
    27601 gctaagtgct gttgtgaaac cattttgcca acgtggcgga tgtttcttgc gccaacgttc
    27661 ataatagcca taacctataa acaatgctgc atggtataaa ccataaacaa tgtaatacac
    27721 ttcgatacca tgccaaattc ccattatgaa gaagttgatt aagaatgcca cgttagacat
    27781 tgcaaattga ctcttcaata attttttacg agacatgtag aataaagatc tcatgtaaat
    27841 acaatctctg aaccagaatg ataatgtcat atgccatcta ttccagaaat ctttaatatt
    27901 tttcgctttg aaaggtttat cgaagtttgg tggtgtttta ataccgaata aataactaaa
    27961 tgctatcgca aataaactat aacctgcaaa gtcaaagaat aaatataggc tgtatgcgta
    28021 catatataac cacaaattga caaagccatg taagtctaat tgtaacggca tgattgcata
    28081 tgtgttaata aagtaagcaa caatatattt atacaagaaa ccaagcataa tcatgtgaat
    28141 tgcttttaat actaattcac gatattcatt gcctgttggt acttttttat cgtctttaac
    28201 gaaacgtttg taacgatcga ttggtccaga tgaaatcgtt gggaagaatg aaataaattg
    28261 aattaatttc catactttaa tttctttgat agaaccatca cgaatttcca taattaactg
    28321 cacacttttg aatgtaacat aagaaattcc taagaaacca acaaattcaa ttaatttact
    28381 ttcatggaag tgaatctgat gtccacctaa ccatgtactt tgtaacactt tcacaagtgc
    28441 taatggcaat attgataaaa ccattacagt tacaaatttt gaaaatgaat tattttttgg
    28501 ttttgaatga taataaaaca ttatcaataa aacttgccat acgacgtaaa taataaaact
    28561 aattaattga acacttaaat acttttggtc aaacagatta tgtttatcag aagaaaagat
    28621 taacacaatc ataaatgctg taactacgcc attataaatg taacttcgct tacctaaaaa
    28681 tccaagtata atgactggta ataatgcaat taaagcaatt aagaagaatg taaaatcacc
    28741 atatggaatc atccgttaat tacctctgca attttctttc tgtcaatttt accatttgaa
    28801 gtcaatggca attgttccat ccattcaaac tttctaggaa tcatatactc tggtaagcgt
    28861 gatttcaagt catttttaat atttttagtc atttctgcat tgtccgtaac ttcagtcgtt
    28921 ggcacaattg caccaattaa atgaataact ttatcatttt tatatacagg tacaacaatc
    28981 gcttctttta cgaactcaga ctggcgtaat tgtgtttcaa tttcttctaa ttccattctg
    29041 tagccattca atttgatttg gaaatcaata cgaccttgaa tgaaccattg accattttca
    29101 aacttcgctt tatcaccagt gtgataagta cgaataccgt catcgaaatt aaatacttca
    29161 gcagtttttt ggtcattttt taagtatcct aaacttacac tttgaccttc gataacaagt
    29221 tcaccttcat ctgtcgtaga taatcttgcg cctggtcttt caacgccaac aggtaatgtc
    29281 ggatattgat ctaagatttc ttgtgtaatt tgaatacttg taactgctac cgtagcttca
    29341 gttggaccat atgtgttgta aatcgtcgca cttgggaaac ggcttactaa cgcttttgct
    29401 gctctgtgag gtagaatttc accacagaag aagaattcgt taagactacc atactgttct
    29461 tcattaagcg ttggtaataa taaacacatt tccataaatg atggtgttga tacccaaatg
    29521 ttaatcggtg ttgctgttag catttcattt aatagtttag gtttattaat catgttttta
    29581 tctacaagat ttaatgtacc gcctgacgct aaacatggat aaatagccat tacagataaa
    29641 tcaaatgaaa atggcgcttg gttaagccat tgttgttcat ttcctgattt attaagttct
    29701 aacatccact cagtaaattg aactaaactt gcatattcaa tttgaacacc tttaggttcc
    29761 ccagtagaac cagatgtaaa gattgtgtat actgtgtcgt tatctttaat ctgactatca
    29821 aaaattactg ggtcttgaga tgttttaata tcttctattg taaatacttc acctgctaaa
    29881 ctttcaaacg attcatcagt cgtattaaat acaaactctg gttgaacctt gttaataatc
    29941 attttaatac ggtcttcagg aattgaagtg tctacaggta catatccaca tcctgcttta
    30001 atggcaccaa tcatcccaac aatcatatat ggtgacatgt gaccgaataa aatcatcggt
    30061 ttcttactac cttgtaatcg atgtgctaat ttactagact catccattaa ctgttgataa
    30121 gttaattcat cagttgtgtg tctaacagca atgctttgtg gatttgcatc cgcaaacgct
    30181 tgcagcttgt taataatatc tgtcatatta agtctccctc attagaactc attataaatg
    30241 aagttattgt gtgtgtcgcc actgccataa attaaatata aagtaataaa tattgccaaa
    30301 tacaatagtg ttaataaata tggtttgaat gcttcaacat atttattagg tggctgttta
    30361 cttttagatt tcatattgca cctcttaaag ttcttagtaa aaacgccttt ataaagaccg
    30421 ttcaatataa aatacgtttt aaaatttgtt ttttacaatt cattatatcg atattcataa
    30481 tgaaattcaa ttttaatttt atagattcaa catagtaatt ggttgtcatc actcaattat
    30541 tgtaaatgat acactatgtt gagaacatca ttcattttaa aggttattta aacaataaac
    30601 aattacagtc tatataacaa ttttgttata tacgtcaaaa tcaaataaac tcatcacatt
    30661 aatatgacga gtttataacg ttattgaatt atcatcagcg caaatatata cattcgcatg
    30721 tcaagcataa catatttaac aattgctttg cttgtttacc aatgattaaa aaccatactt
    30781 attttcaatt tactggatta tgtggtacct gatttgtcat aaccgcttct atattattaa
    30841 tacataattg aatcatattg tcccgtgttg ttactgatgc gctaccaatg tgcggtgtaa
    30901 tcagaacatt atcacgtccc attaatggat gtgtatgatc aattggttca tttgctaata
    30961 catctaaacc acatgctaat atttctttat tgtctagtgc atcgattaat gctgtttcat
    31021 ctacaatttg ccctctaccg atattaataa aaattgcatc atttttcatt tgttcaaatg
    31081 cttcagcatt aaatttatga tgtgtttctt ttgtaagtgg cgctgtacag atgataaaat
    31141 cactttctgc taacaacgtt tcaaaagaaa catatgttgc attaaagtcc gcctctgcat
    31201 ctttatgtct tgatcgatta tgataaagaa ttttagtatt aaacccttgc aaccttcttg
    31261 caaaagcttt accaatatct cccataccat atattccaac agttgaattg aagacatctt
    31321 tacctgacaa taaataagga ccccagcttt gccatgcatc tgcttctaca tatttttcag
    31381 cttctacaat acgacgtgct gtagcaagca ttaatgtaaa tcctaattct gcagttgttt
    31441 cagtaagaac atttggtgta ttcgtgacaa ccacgttgtt cgctgttgca ctttcaacat
    31501 cgatgttgtc atatcctaca gccatattcg caattacttt taaatttggt gattgtgcta
    31561 aaatttctgc atcgatctgt tcacttaatg tgataaaaca agcatctgcg tcacgaagtg
    31621 ctgtcacaaa ttgatcttta ggcataggca ctaatgattt ttgccacata acaacgtcac
    31681 caagtttact caattgttga taaaatttat ctggaatttt cctcgaaaca actattttta
    31741 ccaaatcaat tcacctactt tataaaatga ctatatctat ttttctaatt cagctattgc
    31801 ttcatttaaa tctttaaaag aatacgttgg tggcacattt ttattttgca catcttctaa
    31861 cgtagataca cctgtttgta catgaatcgt atccatacct acgttaatac cagacataat
    31921 atcggtatcg tacaaatcgc ctaccatagc aacttcggat ttatctagtc ctaaaatttc
    31981 taatgcttta accataataa tcggttctgg tttaccaata aattgtggcg atacacctgt
    32041 cgatacactt acaacacttg taatagcacc attccctggt aaaaatcctc gctcttttgg
    32101 aattgacaca tctggatttg tagaaataaa tgttgcccca tttcttacac ctaacgttgc
    32161 aatcgcaagc ttttcatatg taacttgttc gtcaagtcca ataactacat aatcaacatg
    32221 ctcgtcattt ttaatgacaa gtcccgcttc ggttaacgca gtatttaaac cactaccacc
    32281 taacatatat actgatgctc ctggtgattg ttctgaaata taatctgcag tggctaacgc
    32341 tgacgttaca acctcttctg gtttagcatc aatgtgcatt tcacgtaatt tttcagttac
    32401 ttgctcaggt gttttcgttg aattattagt tacgtataaa tgaggaatac cttttacatt
    32461 taaataatcg atgaattgtt ttgctccatc aatctcatct gttcccatat acattgtgcc
    32521 atctaaatcg attaaatacg ctttatactg tttcattatt gattttctcc tttaccaaaa
    32581 gctgttactg gtacattttc ttgatgtaaa aatgtcgtta cttctgtaat aaattgtttg
    32641 taataaggta atgcgtcatc aaacattggc atgataacct taatatctaa ggctgtgtaa
    32701 tcatatgtaa attgtttacg aatatcgaca gttttattaa tcgcctgctg tgtttcttta
    32761 gtaataacat tttctagttc taatatatca atcacatctt tataattacc aggatccctt
    32821 aaaataaatg catcgataat catattccct atatctaccg atgattctat caacatttga
    32881 gcaatgcgtt caaatgcata atgattgtgc ttactctcat gataatcatc agttaatgct
    32941 tgtaaatagg ctaatttctg agttagttta tctttgtcta caaaatacat tccttgtcac
    33001 ctctcgctta tatattatca taattttgca gtatacttca tttgtcgtta tactaacttt
    33061 actactttta aaaggagtcg ctacaaaatg attgatatgt atttatatga tgacaacgaa
    33121 gagagtcaag ttcagtttgt tggttttgtt ggcgagcata gtcgatatga tttaatgcta
    33181 gtccatacaa atagacatta tggtaagaca ctcgtactta atatgcaaac aaataaattc
    33241 ggtattatcg gtactgatga tttaaaagaa gaaggctata ttgctcatat tttaggtgta
    33301 aatgctgaag aaggcgatga aattacagag tatttaaatg aagtcattca ttaaagataa
    33361 aaacccctaa ctagattaca ggtccatgct tgaattgcat gattcccatt ttcaatctag
    33421 ctaagggttt tattaatgtt cagtatcttg aatggttggc ttaatcttca catcttgaag
    33481 tttattgtcg tcatctatca catcagacgg atgaacttct tcgattaact ttttaacctc
    33541 ttgtttagac aaatgtctta atacaaagta aggacaacca aagttacaat attcaaatat
    33601 gtaatcttga atatttgaaa aacgtttact catctctgct tttttattag aatctttgta
    33661 aaaacctttt aatcgtaatt gatcgtaacc atagtcacca acaatgtaat catatttatc
    33721 taaaatatct gaatacctag caataaattg ttcttcatta aaacattcgc gataattttc
    33781 tattaattca aagtaatgtt gatctacttt tatcaaattc ttcacctatc tatgacaatg
    33841 aaaggctcag tttatacttt agtgtttcat aaaaataatg attacttcta tgacacgttc
    33901 gttttgtaca aactaagcct ttatgcctta ttaatggtta aatattaact atttaactgt
    33961 gcctcacctt ggcgttgctt ttctttagca gcttcattta cttgctcatc cgcatgatat
    34021 gaactacgta ctaaaggtcc agcttgacaa tgtttaaacc ctttatccat tgccactttt
    34081 cttaatttac caaactctaa aggcgtgtaa tatttttgaa cctttaaatg tttacgtgaa
    34141 ggttgtaaat attgaccaat cgttaaaata tctacatcat tcgcacgtaa atcatccatc
    34201 gtttcataaa tttcttctat agtttcacct aatccaacca taatacttga tttagttggg
    34261 atatccggtt gtaattcttt tgaacgacgt aaaaactcta atgttctgtc gtatgtcgca
    34321 cgcgcacgaa ctctcggtgt taatcgacga acagtttcaa tattatggtt taaaatgtca
    34381 ggtcttgaag ccattaatgt ttctaacgca tcatagtccc cgcccatatc tgatggtaaa
    34441 atttcaatcg ttgtaaatgg atttctttct ctaactttac gtactgtctc agcataaaca
    34501 tttgaaccag catctcttaa atcatcacgc gcaacagcag tgataacaac gtgtttcaag
    34561 ttcattaatt caactgattc agctacacgt tcaggctcat ttaaatcaag ttcattaggt
    34621 aaacctgtct taaccgcaca aaaacgacaa gctcttgtac atacggcacc taaaatcata
    34681 aatgtcgctg tacgacgtgc accccaacat tcatgtatat taggacattt agcttcttca
    34741 catacagtat taagattttt ttccctcatc atcttcttaa gtcctgtata gttttcgttg
    34801 gtatttaatt ttattttcaa ccaatccggt ttacgtaata tttcctcgtt ttttgtcgcc
    34861 ataacaacac ataccctcct gttaagtgta tctttaaaca ttatatcgaa attcatgaca
    34921 aatgaaaatc caataatctt actttttaat attccaataa tttttcttta aatatatctc
    34981 ttaaaaactc tggcattaaa tactcttttg gtaattcttt caatgttggg aaataacgac
    35041 cgaacgtata catatctatc gtacctaata catattcttt gtcgtcttcg atttcttctc
    35101 cattcagata gtaacgccct gtagaatgaa tgtaccctaa attataaaga atatatcctc
    35161 caaatatatt ccctctgaaa cccaaacctt gtgcatgttc atacatatat tcttgtttat
    35221 tacttttagc taaaatctct tttaatttca caccagaaag tctaaccctt accatattaa
    35281 tcggatgcgg taacatttga tgaatgtcat attcagtcac ttcatctttt acaatatctt
    35341 taacgagtaa gccagcattg atgatagcac attgagcatg tgtatactca caaacacttt
    35401 gagctaataa gtatgcagct tcagttatgt ggttcatact acgcttaagc actactggat
    35461 gttgaattac tggattagac attagcgttt ttccttcttc ttcaaatgaa gttttaactt
    35521 caggtaatgt ttctaaagga ataatctttg cagttttatg tactacttta tgtgcctcaa
    35581 aagttaaatt aacctctcca agataattac catactttcc agctgccgcc atcagtacac
    35641 cattattaat ttcaccatgt tcaaaataat gatgcgtatg actaccaaaa atgacatcaa
    35701 tttcaggcaa ttcttggcat aatgtttcat cgaagaaaat gccacaatga cttagcacga
    35761 ttaatacatc aaatttacct cgttgatgtt caatttcttc ttttatagaa tcaagtggat
    35821 cggtaacaat ccaatctagt gcacgataaa atggggtaaa aggtgctgtc gctgcaacga
    35881 atagtatttt cacaccgtct atatccttaa tataagaaga aacgatatta tttggtaaat
    35941 gacctgattc atctataaca ttgctacatg tcactataaa ttttgcttcg tcgtaaaggt
    36001 gatttaaagc ttcgtgtgaa atggtcatcc cttcattatt accgattgtt gcaacatcac
    36061 attttgcttc atttagtaat gctacattct ttttacctaa agttgcttct gttataggtg
    36121 cggataaatc tacatgatca cctagatcaa catataaaga aggatgatta agtcgtggcc
    36181 gttgttctgc catatatgct ttaatgcgtt cgtattcatg taaatgacta tgaatatcgt
    36241 tcgtatgata aattgtaagc ctcaaattga cgtcctcctt ttaaagttgt attaaatcaa
    36301 cgcacgaata attaaatata tacccattaa caacattact gttctcaata atgttactac
    36361 cgtatctgat ttaattgatt gattcacttt gacaccgatt ttcgcaccaa aataactaga
    36421 tataattaaa atgattgcat aaccccaagc tacgtgacct tgagcaatgt gccctataga
    36481 actcatgaca cttgaaaaga aaatcatcat catacttgtt ccaacagcta catgaggtgg
    36541 aaatctaaat acaataagca ttagtggcgt cattagtgcg ccacctccaa taccaaataa
    36601 acctgtcaat ataccaatta aaaacgttgt aataaaagca aacaatggtg gcacactata
    36661 atgatatgtt ttacctttag cgtctacata agttttttca tactttggtt tatcaaatgt
    36721 tttaaacggt ttaatcttat ttcttaccat taataaaata gctacaaaga ttaaaaagat
    36781 tccaaaatat aaattaaatg actcaaatgt taaatatcta ctaatgaatg acccaagcaa
    36841 tgaacctggt aataatccaa ataaaaagat agagccattt ttaatatcaa cttgttttgt
    36901 ttttagatat ccaagcgatg aagaaagtcc tgtcacaatt agaatgacag acgatgtccc
    36961 aatcgctatt tgcgttgtaa tattatgtag taatccatgt tcaacaccga ggtaaaccat
    37021 tgttggaacg ataataatac cgcctccaat gcctacgatt gaccctataa tcgctgacaa
    37081 accaccgatt agaactaata atgtaattgt taataacata gtcgtcgcct cttaaaataa
    37141 ttttaattgt tgtggtgcta acccttcata ttctatattc aataatcgct gatatgtttt
    37201 ggcattattt gctgcatgac caccagaatt attgttaaat atgacgtaaa ctttcttagc
    37261 tttttgcgct aatatttgtg ccttttgtgc taagtctatt aattcttgct cattataatc
    37321 atataaatag cgtacatctc gccattcttg atctgacata tctttcttag tccaaccata
    37381 atgattacgt ccatgataac gtacaaacgc aatttcattt gtgattcgat tgactaaagg
    37441 tacactgcca tctttcactt gtggttcatc aactactgca tgaatgatct gatgttctgt
    37501 taaaaatgcc aatgtttgtt ctttaaatgc atcactaaac catgattgat gcctaaattc
    37561 tacacacatt ggaaatgctt gtaattgctg tcttacatac aaaatatatt tgatattttg
    37621 tgcattgcag tcaaaccacg gaggaaattg aactaatacc attgctaatt ttttctgtgt
    37681 atgtaagggc tctaacatat ttttaaattg atcaaatagt tcttgccttg tatctgcaaa
    37741 tgttttgtag tctgcatgca atgtgagtgc ttgatgaatt ttgaccacaa attcaaatgt
    37801 atcaggcgtt tcttttatcc atttcaatat atttctttcc ggttgtatcg catagtatgt
    37861 cgcatctaat tcgacaaccg gaaaatgtcc agcatatgtt ttaagtttat cggtttggcg
    37921 ttctaaatct tcatataatg aatagtgatc accccaacct gttaatccga tgtttatcat
    37981 atatatcacc aatgtcatca taccatataa cttttatcat aatcatttca gcgaacttta
    38041 gtttgatgtt ttcgttagat tataatcatt tcagtgaact ttagctcgat aatttcgttt
    38101 gatcataatc atttcagtga actttagctt gatgatttcg tttgatcata aacatttcag
    38161 cgaactttag ctcgataatt tcgtttgact agattggtaa aatagaaacg caatccacaa
    38221 aaatgacaag cactaaaata atgtttgggg gtgcttgtgc ttttgtggat tgcggtcgat
    38281 tatttatatt gcatgatttg attaatttga ttgattatat tggacatgat ggtgttggcg
    38341 ggatgcgttg ttgctagtcg cgggctttgt ccactccaca tatgtgttaa ctctttgtcg
    38401 ccgatgtttg ctgcggcttt tcttatgcta cttgttagct cattttgtat tggataatct
    38461 gggatgtcgc cttcgtattg ggacatttct tcgataaacc tattgttgat accgcgtgca
    38521 agctttccac taaacgcttt tgtaacgact gtatctgttt ctttactatt tataattgca
    38581 tctcgcagta gttctgatgc attactatct tgtgatgtta aaaatgcggt gcccatttgt
    38641 accccttctg cacctaagac aatacttgcc aaaactcctc taccgtccat aattccacca
    38701 gcggcaatga ccggaattga aacgacatct acaatttgtg gcactaaaga tattgttcca
    38761 accataggta attgattttt aggttttaaa aatgaaccac gatgtccacc tgcttcacta
    38821 ccttgagcaa cgatagcatc catacctgct ttttcattcg caatagcttc atcaacactt
    38881 gttgctgtac ctattagctt aatgtttgct tctttcaatc tttttataat agattcattt
    38941 ggaattccaa aagtaaaaca acatacaggc acttgctttt taattatcgt atcaatatga
    39001 cacttaaatt gttgttcttc ggtaattttt acaaccggct cttctaaatg taatgcgcgt
    39061 cgataaggtt ttaaccatgc attcatattt tcaatttgac tactggtata tgattgttga
    39121 cttggtacaa agacatttac accaaaagaa tttgacgtta attggcgtac ataatctatt
    39181 tcgtcttcta attgctgcgt attaaagtaa cctgcgccta ttgtgcctaa tccaccactg
    39241 ttacttactg atgcaactaa tttcggtgtc gtacttcctg ccatacctgc ttgtataatt
    39301 ggatattcaa tacttaacat ttgagtaagt cggttcttat tccacatagc tgttcgctcc
    39361 ttatatagat acgttgcgat ttttccgttg ttgaaattga atttgctgtt gagaaagttt
    39421 ttctttttcc tttttatcca tctcatcttc aatttccata cctaataatt cttcaattaa
    39481 gtcttcatgt gacactatcg cttcagtacc accaaattcg tccaacacaa ttgctaaatg
    39541 ttttctagaa atagtcatct tacgtaatac ccattcagct ttattgtgtt cattcacaaa
    39601 taatggctta gctgaatagt ttgtaatttg atcttctttt ttattactcc aagccaacag
    39661 atatttagaa tgaaacaccc caataatgtt atcaatatct ccctcgtaca ctggatatct
    39721 agtgtatggc ttattcataa ccgtttcata aacttcttcg tatgtcgcat ttgaagcaaa
    39781 tgccgtcaca ttaattctag gtgttgtatc aacatctttt acttttaaat tttcaaaatt
    39841 aatgacacct tccaacctac tcgtctcaat ttcatttaaa gcaccttcat gtccagcaat
    39901 tgctaacatt gttttaaatt cttcttttga aaattgatgt tcttgaggtt gacccttaga
    39961 taaacttcga ttaatactgt ccgtcaactt atttaaaagt aatgtgatag gacggaacac
    40021 aatgacacaa atatttataa ttggatatac aagccttgtt attttatctg gaaatgtcgc
    40081 agcgacagac ttgggaatca cttcggaaat caaaatgata acaactgtta aaacagctga
    40141 tgcaatccca acgctaatcc cccaacgtaa agccataatt gtaacaagtg ttggtaataa
    40201 aatattcgcg acattattcc caattagaat agttgtaata aactcacttg gtttttcaag
    40261 taactttaca atgccttttg cttttttatc acctttgtca gcttcagttt taaattttgt
    40321 tttattggca gccgttaatg ccgtctcgct tcctgaaaag aaaaacgaaa taaatatcaa
    40381 tataattatg gcaatgatca cgcgtgtagt ctccttattg tcatatctta tttttattgt
    40441 agttacctaa ttcccgacgt tctcgtttaa ta
//
LOCUS       DAKSQE010000010        40341 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.010, whole genome shotgun sequence.
ACCESSION   DAKSQE010000010 DAKSQE010000000
VERSION     DAKSQE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 40341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40341
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.010"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            14..796
                     /gene="modA"
                     /locus_tag="O0V33_000388"
     CDS             14..796
                     /gene="modA"
                     /locus_tag="O0V33_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCX8975073.1"
                     /translation="MKMKRFIAIVMALFLVLAGCSNSNDNQDNKKDAADNGKKQEIQV
                     AAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVD
                     ALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKGNDKLALGEVKTVPAGKYAKQY
                     LDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEV
                     ELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFTA"
     gene            810..1481
                     /gene="modB"
                     /locus_tag="O0V33_000389"
     CDS             810..1481
                     /gene="modB"
                     /locus_tag="O0V33_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCX8975074.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTILGIFISKWLYRRKGSWVKVLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGLRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            1482..2087
                     /locus_tag="O0V33_000390"
     CDS             1482..2087
                     /locus_tag="O0V33_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCX8975075.1"
                     /translation="MLKINLKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTAKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSEHIDQLIQTLNIDHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            2255..3259
                     /locus_tag="O0V33_000391"
     CDS             2255..3259
                     /locus_tag="O0V33_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HCX8975076.1"
                     /translation="MSQERYSRQILFKQIGEIGQNKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDY
                     IDHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKHRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWEGSHYSFGFSKMQRTACTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENTSITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            3288..3794
                     /locus_tag="O0V33_000392"
     CDS             3288..3794
                     /locus_tag="O0V33_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HCX8975077.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRNFDTDKGGQCVLQLLQVDNV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDVDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     EGFGELFRYLSYVEDVGTRALLSRAVAGTVNDKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(3796..4290)
                     /gene="moaC"
                     /locus_tag="O0V33_000393"
     CDS             complement(3796..4290)
                     /gene="moaC"
                     /locus_tag="O0V33_000393"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCX8975078.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNETIYKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTIIPMCHPLPLTGIDVHFNWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QLDQ"
     gene            4353..5612
                     /locus_tag="O0V33_000394"
     CDS             4353..5612
                     /locus_tag="O0V33_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCX8975079.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSTMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETTTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQQDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFADGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            5609..6094
                     /gene="mobB"
                     /locus_tag="O0V33_000395"
     CDS             5609..6094
                     /gene="mobB"
                     /locus_tag="O0V33_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCX8975080.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKSHGYTVATIKHHGHGKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRNEEELQVLQQLTNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            6108..6554
                     /locus_tag="O0V33_000396"
     CDS             6108..6554
                     /locus_tag="O0V33_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HCX8975081.1"
                     /translation="MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            6560..6793
                     /gene="moaD"
                     /locus_tag="O0V33_000397"
     CDS             6560..6793
                     /gene="moaD"
                     /locus_tag="O0V33_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HCX8975082.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFENLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            6800..7399
                     /gene="mobA"
                     /locus_tag="O0V33_000398"
     CDS             6800..7399
                     /gene="mobA"
                     /locus_tag="O0V33_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCX8975083.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NVYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            7419..8441
                     /gene="moaA"
                     /locus_tag="O0V33_000399"
     CDS             7419..8441
                     /gene="moaA"
                     /locus_tag="O0V33_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCX8975084.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATADGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
     gene            complement(8722..8892)
                     /locus_tag="O0V33_000400"
     CDS             complement(8722..8892)
                     /locus_tag="O0V33_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975085.1"
                     /translation="MFRFISFGQLVVYSTTFIPKKDILQLNDLECDNNVKEPLKIYYV
                     VILKSGIEMIFS"
     gene            9018..9368
                     /gene="sarV"
                     /locus_tag="O0V33_000401"
     CDS             9018..9368
                     /gene="sarV"
                     /locus_tag="O0V33_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HCX8975086.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            complement(9470..10681)
                     /locus_tag="O0V33_000402"
     CDS             complement(9470..10681)
                     /locus_tag="O0V33_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCX8975087.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFFVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYAIDLLFIICVILGILGVIISLFIKVDFEVTNSKTETNVIDKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFAIELHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(10674..11114)
                     /locus_tag="O0V33_000403"
     CDS             complement(10674..11114)
                     /locus_tag="O0V33_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HCX8975088.1"
                     /translation="MLSQEFFNSFITIYRPYLKLTEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHEILLKEAHND"
     gene            11277..12041
                     /locus_tag="O0V33_000404"
     CDS             11277..12041
                     /locus_tag="O0V33_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCX8975089.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNDIEAVKN
                     KLQSEQVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKK
                     LQKYFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIE
                     DGKVSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            complement(12033..12284)
                     /locus_tag="O0V33_000405"
     CDS             complement(12033..12284)
                     /locus_tag="O0V33_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975090.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIALAIIHMGILAHSTYVYLY"
     gene            12673..13938
                     /gene="femX"
                     /locus_tag="O0V33_000406"
                     /gene_synonym="fmhB"
     CDS             12673..13938
                     /gene="femX"
                     /locus_tag="O0V33_000406"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HCX8975091.1"
                     /translation="MEKMHITNQEHDAFVKSNPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEIQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMETSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            14055..17222
                     /locus_tag="O0V33_000407"
     CDS             14055..17222
                     /locus_tag="O0V33_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCX8975092.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTSMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKTSASNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVTTIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHADGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKIDQNKAAENGISASQLAMHLNENLPKKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(17409..17726)
                     /gene="mspA"
                     /locus_tag="O0V33_000408"
     CDS             complement(17409..17726)
                     /gene="mspA"
                     /locus_tag="O0V33_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HCX8975093.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(17971..18135)
                     /locus_tag="O0V33_000409"
     CDS             complement(17971..18135)
                     /locus_tag="O0V33_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975094.1"
                     /translation="MSLENQLAELKYDYVRLQGDLEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            18268..19176
                     /locus_tag="O0V33_000410"
     CDS             18268..19176
                     /locus_tag="O0V33_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCX8975095.1"
                     /translation="MTEQFTMIILLIALGYFLKRINYIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSLSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFGMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNISHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVHVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     PFIGMTTNLTIIISIIILYVYQAVFV"
     gene            19347..19487
                     /locus_tag="O0V33_000411"
     CDS             19347..19487
                     /locus_tag="O0V33_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975096.1"
                     /translation="MYGAYLINEYHQDFKSIANFKVLNRMINVMMCYCVLFLEPCISW
                     DI"
     gene            complement(19595..20458)
                     /locus_tag="O0V33_000412"
     CDS             complement(19595..20458)
                     /locus_tag="O0V33_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein GlcU"
                     /protein_id="HCX8975097.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWVGIIII
                     VIAAIILGNLK"
     gene            complement(20639..21526)
                     /locus_tag="O0V33_000413"
     CDS             complement(20639..21526)
                     /locus_tag="O0V33_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCX8975098.1"
                     /translation="MSIVQLYDITQIKSFIKHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVETFETLFKTMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            21592..21687
                     /locus_tag="O0V33_000414"
     CDS             21592..21687
                     /locus_tag="O0V33_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975099.1"
                     /translation="MILTCYNTFNTSFAIIEFKSKISGIDEARSK"
     gene            21713..23848
                     /locus_tag="O0V33_000415"
     CDS             21713..23848
                     /locus_tag="O0V33_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCX8975100.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPLRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYYTLSL
                     TVKGFDFQLESNQRYTNKETLEQIVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMSPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            23961..25295
                     /locus_tag="O0V33_000416"
     CDS             23961..25295
                     /locus_tag="O0V33_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCX8975101.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
     gene            complement(25488..25877)
                     /locus_tag="O0V33_000417"
     CDS             complement(25488..25877)
                     /locus_tag="O0V33_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975102.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLLFN
                     ALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVL
                     VVLSAPTYIPNKNARVIYTQYKDGHRE"
     gene            26234..26542
                     /gene="rpsJ"
                     /locus_tag="O0V33_000418"
     CDS             26234..26542
                     /gene="rpsJ"
                     /locus_tag="O0V33_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCX8975103.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            26570..27232
                     /gene="rplC"
                     /locus_tag="O0V33_000419"
     CDS             26570..27232
                     /gene="rplC"
                     /locus_tag="O0V33_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCX8975104.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            27259..27882
                     /gene="rplD"
                     /locus_tag="O0V33_000420"
     CDS             27259..27882
                     /gene="rplD"
                     /locus_tag="O0V33_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCX8975105.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKVQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            27882..28157
                     /gene="rplW"
                     /locus_tag="O0V33_000421"
     CDS             27882..28157
                     /gene="rplW"
                     /locus_tag="O0V33_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCX8975106.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            28190..29023
                     /gene="rplB"
                     /locus_tag="O0V33_000422"
     CDS             28190..29023
                     /gene="rplB"
                     /locus_tag="O0V33_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCX8975107.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            29090..29368
                     /gene="rpsS"
                     /locus_tag="O0V33_000423"
     CDS             29090..29368
                     /gene="rpsS"
                     /locus_tag="O0V33_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCX8975108.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            29397..29750
                     /gene="rplV"
                     /locus_tag="O0V33_000424"
     CDS             29397..29750
                     /gene="rplV"
                     /locus_tag="O0V33_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCX8975109.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            29774..30427
                     /gene="rpsC"
                     /locus_tag="O0V33_000425"
     CDS             29774..30427
                     /gene="rpsC"
                     /locus_tag="O0V33_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCX8975110.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            30430..30864
                     /gene="rplP"
                     /locus_tag="O0V33_000426"
     CDS             30430..30864
                     /gene="rplP"
                     /locus_tag="O0V33_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCX8975111.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            30854..31063
                     /gene="rpmC"
                     /locus_tag="O0V33_000427"
     CDS             30854..31063
                     /gene="rpmC"
                     /locus_tag="O0V33_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCX8975112.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            31087..31350
                     /gene="rpsQ"
                     /locus_tag="O0V33_000428"
     CDS             31087..31350
                     /gene="rpsQ"
                     /locus_tag="O0V33_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCX8975113.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            31382..31750
                     /gene="rplN"
                     /locus_tag="O0V33_000429"
     CDS             31382..31750
                     /gene="rplN"
                     /locus_tag="O0V33_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCX8975114.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            31786..32103
                     /gene="rplX"
                     /locus_tag="O0V33_000430"
     CDS             31786..32103
                     /gene="rplX"
                     /locus_tag="O0V33_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCX8975115.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            32130..32669
                     /gene="rplE"
                     /locus_tag="O0V33_000431"
     CDS             32130..32669
                     /gene="rplE"
                     /locus_tag="O0V33_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCX8975116.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            32692..32877
                     /locus_tag="O0V33_000432"
     CDS             32692..32877
                     /locus_tag="O0V33_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCX8975117.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            32909..33307
                     /gene="rpsH"
                     /locus_tag="O0V33_000433"
     CDS             32909..33307
                     /gene="rpsH"
                     /locus_tag="O0V33_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCX8975118.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            33332..33868
                     /gene="rplF"
                     /locus_tag="O0V33_000434"
     CDS             33332..33868
                     /gene="rplF"
                     /locus_tag="O0V33_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCX8975119.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            33899..34258
                     /gene="rplR"
                     /locus_tag="O0V33_000435"
     CDS             33899..34258
                     /gene="rplR"
                     /locus_tag="O0V33_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCX8975120.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            34279..34779
                     /gene="rpsE"
                     /locus_tag="O0V33_000436"
     CDS             34279..34779
                     /gene="rpsE"
                     /locus_tag="O0V33_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCX8975121.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            34796..34975
                     /gene="rpmD"
                     /locus_tag="O0V33_000437"
     CDS             34796..34975
                     /gene="rpmD"
                     /locus_tag="O0V33_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCX8975122.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            34992..35432
                     /gene="rplO"
                     /locus_tag="O0V33_000438"
     CDS             34992..35432
                     /gene="rplO"
                     /locus_tag="O0V33_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCX8975123.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            35432..36724
                     /gene="secY"
                     /locus_tag="O0V33_000439"
     CDS             35432..36724
                     /gene="secY"
                     /locus_tag="O0V33_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCX8975124.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            36741..37388
                     /locus_tag="O0V33_000440"
     CDS             36741..37388
                     /locus_tag="O0V33_000440"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCX8975125.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            37581..37799
                     /gene="infA"
                     /locus_tag="O0V33_000441"
     CDS             37581..37799
                     /gene="infA"
                     /locus_tag="O0V33_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCX8975126.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            37831..37944
                     /gene="rpmJ"
                     /locus_tag="O0V33_000442"
     CDS             37831..37944
                     /gene="rpmJ"
                     /locus_tag="O0V33_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCX8975127.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            37967..38332
                     /gene="rpsM"
                     /locus_tag="O0V33_000443"
     CDS             37967..38332
                     /gene="rpsM"
                     /locus_tag="O0V33_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCX8975128.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            38356..38745
                     /gene="rpsK"
                     /locus_tag="O0V33_000444"
     CDS             38356..38745
                     /gene="rpsK"
                     /locus_tag="O0V33_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCX8975129.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            38820..39764
                     /locus_tag="O0V33_000445"
     CDS             38820..39764
                     /locus_tag="O0V33_000445"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCX8975130.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            39781..40149
                     /gene="rplQ"
                     /locus_tag="O0V33_000446"
     CDS             39781..40149
                     /gene="rplQ"
                     /locus_tag="O0V33_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCX8975131.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
BASE COUNT        14598 a         6042 c         7721 g        11980 t
ORIGIN      
        1 ggggtatttc ataatgaaaa tgaaacgttt tatagctatt gtaatggcat tatttttagt
       61 attagctggt tgttctaatt ctaatgataa tcaagacaac aaaaaagatg cagcagacaa
      121 tggaaaaaaa caagaaattc aagttgcagc ggcagcaagt ttaacagatg ttacaaaaaa
      181 attagcatca gaatttaaaa aagaacataa aaatgctgat attaaattta actatggtgg
      241 atcaggagca ttaagaaaac aaattgaatc aggcgcacct gttgacgtat ttatgtctgc
      301 aaatactaaa gatgtagatg cattaaaaga caagaataaa gcacatgata catataaata
      361 tgcgaaaaat agtctagtat taattggtga taaagattca aattacactt cagtaaaaga
      421 cttaaaaggc aatgataaat tagcattagg tgaagtgaaa actgtaccag caggaaaata
      481 tgcgaaacag tatttagata acaataactt gtttaaagaa gttgaaagta aaatcgttta
      541 tgctaaagat gtaaaacaag tattaaatta tgttgaaaaa ggtaatgcga aacaaggttt
      601 tgtatataaa actgacttat ataaacaaaa taaaaaaatt gatactgtaa aagtaattaa
      661 agaagtagaa cttaagaaac caatcacata tgaagctggt gcgacatcag atagtaaatt
      721 agctaaagag tggatggaat tcttaaaatc agataaagct aaagaaatat taaaagaata
      781 ccactttaca gcataaggag ttgtaatcca tgcctgactt aacacctttt tggatatcaa
      841 tacgagttgc tgtaatcagt acgattattg taacgatttt aggtattttt atatctaaat
      901 ggttgtatcg tcgtaagggt tcgtgggtta aagtattgga aagtttattg atattaccta
      961 ttgttttgcc gccaacggta ttaggtttta ttctattaat catcttctcg ccaagaggac
     1021 caattggtca attctttgcg aatgtactac atttacctgt agtattcact ttgacaggtg
     1081 ctgtgatagc atctgtcatt gttagttttc cactaatgta tcaacatact gtgcaaggcc
     1141 tcagaggtat agacacgaaa atgattaata cagctagaac gatgggagca agtgaaacga
     1201 aaattttcct caaattaatt ttaccattag ctaaacgctc tattttagca ggtataatga
     1261 tgagttttgc tcgtgcatta ggtgagtttg gtgctacatt aatggttgca ggatatattc
     1321 caaataaaac gaatacacta cctttagaaa tatacttctt agtggaacaa ggtagagaaa
     1381 atgaagcgtg gttatgggta ttagtgctag tcgcattctc tattgtggtt atatctacaa
     1441 ttaatttatt gaataaagat aaatataagg aggtcgacta gatgcttaaa atcaatttaa
     1501 aatatcaatt aaagaatact ttaattcgca tcaatataga tgatactgaa ccaaaaattt
     1561 atgcagttcg tggtccatct ggcattggta aaactactgt tttaaatatg attgccggat
     1621 tacgtaaagc agatgaagct attatcgaag tgaatgggca attgcttact gatacggcaa
     1681 aaaacgtgaa tgttaaaatt caacaacgac gtattggata tttatttcaa gactaccaac
     1741 tgtttcctaa tatgacggtc tataaaaata ttacttttat ggctgaacca tctgaacaca
     1801 tcgatcaatt aatccaaact ttaaatattg atcatttgat gaaacaatat cctatgacat
     1861 tgtcaggtgg agaggcacaa cgtgtagcac ttgcacgtgc acttagcacg aaaccagatt
     1921 taattttatt agatgaacct ttttctagtt tggatgatac tacaaaagat gagagtatta
     1981 cattagttaa acgtattttc aacgaatggc aaataccaat catatttgtg acacattcaa
     2041 actatgaagc agaacaaatg gctcatgaaa ttattacaat tggttaatga tacatttgcc
     2101 ttgaaagagt ttagaacgta tttaaaaatg tagaagtgca atcttctatt agcattttaa
     2161 tgatgtttta aactcttttt taggcgcaat ttttgagaga cattgacgca cgtcatataa
     2221 tgaaagtaag gataaataga aaggataact taatgtgagt caagaacgtt attcaaggca
     2281 aattttattt aaacaaatag gtgaaatagg tcaaaacaaa ataaatcaaa aatgcgcgtt
     2341 gattattggt atgggcgcat tgggtacaca tgtggctgaa ggtcttgtga gagcaggcat
     2401 tgctaaacta atcattgttg acagagatta tattgaattt agtaatttac aaagacaaac
     2461 attatttact gaagaagatg ctttgaaaat gatgcctaaa gtggttgcag ctaaaaagca
     2521 tttgctagcg ttacgtagtg atgttgatat tgatgattat attgaccatg tggattatta
     2581 ttttttggag acacatggac aggacgttga cgttatcatt gatgcaaccg ataactttga
     2641 aacacgacaa ctgattaatg attttgcata taaacatcgt ataccttgga tttatggagg
     2701 tgttgtacag agtacatata cagaagctgc atttatacct ggtaaaacac cttgctttaa
     2761 ctgtttggta ccacaattgc cagcattaaa tttaacatgt gatacagtag gggtcattca
     2821 acctgccgtg acgatggcaa caagtttaca attaagagat gcgatgaaag tattaacgga
     2881 acaaccaatt gacacaaaaa taacttatgg cgatatttgg gaaggtagtc attattcatt
     2941 tggtttcagt aaaatgcaac gtacagcctg tacaacttgt ggagatgtac caagttatcc
     3001 gtatttaaac aagaatgaac aacgttatgc aacattgtgt gggagagaca ctgtacagta
     3061 tgaaaataca tcaattacac acgatattct tgttcaattt ttaaaacagc atcaactaaa
     3121 ttatcgcagt aattcatata tggttatgtt tgaatttaaa gggcaccgca ttgttgcttt
     3181 taaaggtgga aggtttttaa tacatggcat gacacgcaca tcagatgcca cacatctaat
     3241 gaatttattg tttggataaa aaggtaaaac aaaaggagtg taatattatg ggcgaacatc
     3301 aaaacgttaa attgaatcgt acagttaaag cagccgtact aacggtatca gatactagaa
     3361 actttgatac agataaaggt ggtcaatgcg tgctccaact gttacaagta gataatgttg
     3421 aagttagcga tgcacattat accattgtaa aagacgaaaa agtagctatt acgacgcagg
     3481 tgaagaagtg gttagaagaa gatgttgatg tcatcattac gactggtgga acgggtattg
     3541 cacaacgtga tgtaacaatt gaagcagtaa aaccgctttt aactaaagag atagaaggct
     3601 ttggagaatt atttagatat ttgagttatg ttgaagatgt tggcacgcgt gcattattat
     3661 ctcgcgcagt ggcaggtaca gtaaatgata aattgatatt ttcaattcca ggatcaacag
     3721 gtgcagttaa attagcatta gaaaagctca ttaaaccaga attaaatcat ctgattcatg
     3781 agcttacaaa ataatttatt gatctaattg gcgttgaaaa tctccagatt taccgccaga
     3841 cttgctttca aggtaggttt cgccaataat cataccttta tcaactgctt ttgtcatgtc
     3901 gtaaatggtt aaagccgttg ctgatgcagc ggttaaagct tccatttcaa caccggtttt
     3961 gccagttgta gagacagttg tttgaatgtt taaagtataa aggggtgcat ttgtttcatc
     4021 ccagttgaag tgaacatcta tgccagtcaa tggtaatgga tgacacatcg gaataattgt
     4081 tgatgtattt ttggcagcca taataccagc gatttgagca gtgttcaata cattgccttt
     4141 ggtattggtg ttattagaaa tctgcttata aatagtttca tttacagtaa tgcttgaatg
     4201 agcgacagca gttctttttg taatttgttt gtctgataca tcgaccattt tggcgtggcc
     4261 ttgttgatta atatgagtaa actcagtcat tttacccctc ctagtgcatc tagtatatca
     4321 tgaaaaaata aaagttttgg agatgatttt taatggtagt agaaaaaaga aacccaatcc
     4381 cagttaaaga agcaattcaa cgtatcgtta atcagcagag tacaatgccg gcaattacgg
     4441 tagcacttga aaaaagtcta aatcatattt tagcagaaga tattgtagct acttatgata
     4501 taccaagatt tgataaatca ccttatgatg gttttgcaat tcgcagtgtt gattcacaag
     4561 gggcaagtgg tcagaatcga attgagttta aagtgattga tcatattggt gcaggttcag
     4621 tttctgataa attagttggg gatcacgaag cggtgcgtat tatgactgga gcacaaatac
     4681 ctaatggcgc agatgctgtt gttatgtttg aacaaacgat tgaactagaa gatacattta
     4741 caattcgtaa accattttca aaaaatgaaa atatatcttt aaaaggtgaa gaaacaacga
     4801 caggcgatgt tgttctaaaa aaaggacaag taattaatcc aggggctatt gcggtccttg
     4861 caacatatgg ctatgcagag gttaaagtta ttaagcaacc gagcgtcgct gttattgcaa
     4921 caggaagcga attattagat gttaatgatg tattagaaga tggaaaaatt cgtaactcta
     4981 atggtccaat gattcgtgcc ttagcagaaa aattaggtct tgaagttggt atttacaaaa
     5041 cacaacaaga tgatttagat agtggtatac aagtcgttaa agaagctatg gaaaaacacg
     5101 atatcgttat tacaacgggc ggagtttctg ttggagactt tgactattta cctgagattt
     5161 ataaggctgt aaaggcggaa gtgttattta ataaagtagc aatgcgtcct ggtagcgtta
     5221 caacggttgc atttgcagat ggaaagtatt tgtttggatt atctggaaat ccatcagctt
     5281 gttttacagg atttgaacta tttgtgaaac cagctgttaa acatatgtgt ggcgcactag
     5341 aagtcttccc gcaaataatt aaagcaacat taatggaaga ttttaccaag gcaaacccat
     5401 tcacacgatt tatacgtgct aaagcaacgt taacaagtgc tggagctact gtagtacctt
     5461 caggattcaa taaatcaggt gcagttgtag cgattgcaca tgctaactgt atggtcatgt
     5521 taccaggagg gtcacgtggt tttaaagcgg gtcatacagt agatattata ttgactgaat
     5581 ctgacgctgc tgaagaggaa cttcttttat gattttacaa attgtaggtt acaaaaagtc
     5641 tggtaagaca acattgatga ggcatattgt ctctttctta aagtcacatg gttatacagt
     5701 tgctactatt aaacatcatg ggcatggtaa ggaagatatt caattacagg attcagacgt
     5761 cgatcacatg aagcattttg aagcgggggc agatcaaagt attgtacaag gatttcaata
     5821 tcagcaaact gtaacacgtg tagataatca aaatcttact caaattattg agaaatctgt
     5881 tacaattgac accaatatcg tattagttga aggctttaaa aatgctgatt ttgaaaaagt
     5941 cgtagtctat cgaaatgaag aagagttgca agtattacaa caattgacga atgtttgtta
     6001 tagcattaat gtaagggagc atgaagattt tacagcattt gagcaatggt tattaaataa
     6061 aattaaaaat gattgtgata cacaattaac atagaggatt gaaatgaatg aaacaatttg
     6121 aaatcgtgac agaaccgata caaacagaac aatatcgtga attcactata aatgaatatc
     6181 aaggtgcagt agttgttttt accggtcatg ttcgcgaatg gactaaaggc gtcaaaacgg
     6241 aatatttaga atatgaagcg tatattccaa tggctgaaaa gaaattggca caaattggag
     6301 atgaaataaa tgaaaaatgg cctggaacga taacgagtat tgttcataga atagggccat
     6361 tacaaatttc agatatcgct gtattaattg cagtttcttc accgcatcgt aaagatgcct
     6421 atcgagcaaa tgaatatgca attgagcgta tcaaagaaat tgttccgatt tggaaaaaag
     6481 aaatttggga agatggttca aaatggcaag gacatcaaaa agggaattat gaagaagcga
     6541 agagggagga ataagagaga tgaaggtact ttacttcgca gaaattaaag atatattaca
     6601 aaaagcacag gaagatattg tgcttgaaca agcattgact gtacaacaat ttgaaaattt
     6661 attgtttgaa cgttatccgc aaatcaataa taaaaagttt caagttgctg taaatgagga
     6721 atttgtacaa aaatccgatt tcattcaacc taatgatacc gttgcattaa ttccaccggt
     6781 tagtggaggt taagcgagca tgaaagcaat aattcttgca ggtggtcatt cagtaagatt
     6841 tggtaagccc aaagcttttg cggaagtgaa cggtgagacc ttttatagta gagtaattaa
     6901 gacattagaa tcaacaaata tgttcaatga aattattatt agtacaaatg cgcaattggc
     6961 aacgcaattt aaatatccaa atgttgttat agatgatgag aatcataatg ataaaggtcc
     7021 attagcagga atttatacaa tcatgaagca acatcctgaa gaagaattgt tttttgtcgt
     7081 ttctgttgat acaccaatga ttactggtaa agctgtaagc acgttgtatc agtttttagt
     7141 ttctcatctt attgaaaatc atttagatgt cgcagctttt aaagaagatg gacgttttat
     7201 tccaacaatt gcattttata gtccgaatgc attaggcgct ataactaaag cactacattc
     7261 tgataattac agttttaaaa atgtatatca tgaattatca actgattatt tggatgtcag
     7321 ggatgtagat gcgccctcat attggtacaa aaatataaat tatcagcatg atttggacgc
     7381 tttaattcaa aaattgtaag ccgttaggag gtccacaaat ggtagaacaa ataaaagata
     7441 aactaggacg tcccatccgt gacttacggt tatctgtgac agatcggtgt aactttaggt
     7501 gtgattattg catgcctaaa gaggtatttg gagatgattt cgtattttta cctaaaaatg
     7561 aactgttaac gtttgatgaa atggctagaa tcgctaaggt atatgcagaa ttaggtgtaa
     7621 aaaaaatacg cattacaggt ggagaaccat tgatgcgacg ggatttagat gtacttattg
     7681 ctaaattaaa tcaaatcgat ggtattgaag atattggttt gactacaaat ggtttgttat
     7741 taaaaaagca tggacaaaag ttatatgatg ctgggctacg cagaattaat gtcagtttgg
     7801 atgctattga tgatacgcta tttcaatcaa tcaataatcg taatattaaa gcgactacga
     7861 ttttagaaca aattgattac gcgacgtcta ttggtttgaa tgtaaaagta aatgttgtta
     7921 tacaaaaagg tattaacgat gatcaaatta taccaatgct tgaatatttt aaagataaac
     7981 atatagagat tcgatttata gaatttatgg atgttggtaa tgataatgga tgggatttca
     8041 gcaaagttgt aactaaagat gaaatgctta ctatgataga gcagcacttt gaaattgatc
     8101 ctgtagaacc aaaatatttt ggggaagtag caaaatatta tcgacataag gataatggtg
     8161 ttcaatttgg tttgattaca agtgtttcac aatcattttg ttctacatgt acacgggcaa
     8221 ggctgtcatc agatgggaag ttttacggat gtttatttgc aactgccgat ggatttaacg
     8281 ttaaagcgtt tattcgttct ggcgtgaccg acgaagaatt aaaagaacaa tttaaagctt
     8341 tatggcaaat aagagatgat cgatattcag atgagagaac tgctcaaaca gttgccaatc
     8401 gtcaacgtaa aaagataaac atgaattata ttggtggtta atgtgtagga accactacat
     8461 attaaatcat tagagattct ttaatatttc tgtcttactc cctaaaatac aatattattt
     8521 attaaagtaa aaacggtcat atctatgcca aatttaatag aaatgatcgt tttaaatatt
     8581 tttacaaatt ggcggggccc caacatagaa ggtgacgaaa agtcagctta caataatgtg
     8641 caagttggcg gggccccaac atagagaatt tcaaaaagaa attctacaga caatgcaagt
     8701 tggggaacaa cgaaataaat tttatgaaaa tatcatttct atcccactct taagaatcac
     8761 tacataataa atctttagtg gctctttaac attgttgtca cactccaagt cattgagttg
     8821 taatatatct tttttaggta taaatgttgt cgaataaaca acaagttgtc caaaagatat
     8881 aaatctaaac aagatatagc cagcaattta atatttgtaa tagataaaat gctaagtttg
     8941 atatataata aatttaagta attgtataat aatatgaatt acaaacatct aagaagaaac
     9001 ataggaggca tcatattatg agtaataaag ttcaacgttt tatagaagca gaaagggagt
     9061 taagtcagtt aaagcactgg ttaaaaacaa cacataagat ttcaattgaa gaatttgtag
     9121 tactttttaa agtgtatgaa gctgaaaaga ttagcggtaa agaattgagg gatacattac
     9181 attttgaaat gctatgggat acaagtaaaa tcgatgtgat tatccgtaaa atctataaaa
     9241 aagagcttat ttctaaattg cgttctgaaa cggatgaaag acaagtattc tatttctata
     9301 gtacttctca aaagaaattg ttagataaaa ttactaaaga aatagaagtg ttaagcgtta
     9361 caaactaaaa acttaaaaag catgccaatc agtacatcat aattgcgtct tggggacaga
     9421 caaatgatga atagagattg gcatgctttt tatttttgaa tataaatatt tagttcatgg
     9481 catttctagt tacatgacgt ccatgaatta agaagtaaac aagcatagta atgattgcta
     9541 aagcggccat aaaaccgaag atttcactat atgaaaacat atgagtaaat aacccaagga
     9601 atgatggacc gaagccgaca cctgcatcta gaccaacgta aaaagtagat gtcgcgatac
     9661 catatttaat cgggggtgag acttttatcg caatagattg cattgcagat gataaatttc
     9721 cataccctaa acctaggcaa gcaccagcaa gtaatattaa ccagctttga tagcttgaaa
     9781 ttaagcacac aaatgaaagg aaaagcatga taaatgctgg gtagacaata atattttcat
     9841 ttttatcatc catcaatcta ccagcaatag gtctagtaat taacgatgct atagcatagc
     9901 aaataaagaa atagcttgct gcagttacta ggtgtagctc tatagcaaat gcttgtaaat
     9961 aagttaggat ggacgcatag gtaacgccaa ttaaaagcat aataacagca acaggaatgg
    10021 cctcttttgc aataaattga tgaatactaa atcttggttt atcaatgaca ttagtttcag
    10081 ttttgctatt tgttacttcg aaatcaactt ttataaataa tgagataatg actccgagta
    10141 tgcctaatat aacacaaata ataaacagta aatcaattgc gtattttgta ataagtaaca
    10201 tgcctagaaa tgggccaatc gctgtaccta atactaaact taaagaaaat aaactgatgc
    10261 cttcactttt tctattaaca ggggtaacgt atgccgcaat agtacctgtt gcagttgtca
    10321 caactgcagt tgcgataccg tttatgagac gtacaaagat taaaaaagct aaagatccat
    10381 caataaaata aagtaattgc gtgataatta aagcaattaa accaataaat aataatcgtt
    10441 taggtccgat ttgatttaca aatttacctg tagcaaatcg accaattaaa gaaccgacaa
    10501 taaatagtcc tacaactaat ccggctaatg aatcggaagc gtcgaaagct tgcttactat
    10561 agccagcaat aactacaagt aacaaataca tgcataggta tacgataaaa ttgacaaaaa
    10621 agttaattgt aaaacttttg gtgaaaatag gtgatttatt taatgagcct tgattagtca
    10681 ttgtgtgcct cctttaataa tatctcatga atttgattca tcacatttat ggtttcttgc
    10741 atttgatctt gtgaaatgtt tgttttggca actactgctt gttggagctt ttgtacatca
    10801 agacaaacaa tctcatataa ttcatgccct ttaggtgtta aagttaaaaa cttttgtcgt
    10861 ttatcctcta aactgttttc tactgttata aggtcatttt ctattagagc ttttaaagtt
    10921 tttcttgcag taggcttttc aattgcacgt ctatgtgaaa tttcaatgag agtagtgggt
    10981 tgatgtttag cgatatcgcg taagattaac cattgaccat aatatatatt gtgtttttcc
    11041 aaaataggct ctgttaattt taaatacggg cgatatattg ttataaaact attgaaaaat
    11101 tcttgtgata gcatagcgac atctcctaag acaaaatagt tagcttagct aacctttttt
    11161 acaacaatag taattataaa acggggacaa ttagaaatca atatataatt attaagagca
    11221 aaaatattta tactttgtta aaataagcgt aattacatgt aaataggggg atactaatga
    11281 tattgaaatt tgatcacatc attcattata tagatcagtt agatcggttt agttttccag
    11341 gagatgttat aaaattacat tcaggtgggt atcatcataa atatggaaca ttcaataaat
    11401 taggttatat caatgaaaat tatattgagc tactagatgt agaaaataat gaaaagttga
    11461 aaaagatggc aaaaacgata gaaggtggag tcgcttttgc tactcaaatt gttcaagaga
    11521 agtatgagca aggctttaaa aatatttgtt tgcgtacaaa tgatatagag gcagttaaaa
    11581 ataaactaca aagtgagcag gttgaagtag taggaccgat tcaaatggaa agagatacac
    11641 ataaagatgg taaggtaaag tggcaattgc tttatataat gaatcaggat gatgatgaaa
    11701 ttaagccacc attttttatt caatgggaag aaagtgattc catgcgtact aaaaaattac
    11761 aaaaatattt tcaaaaacaa ttttcaattg aaactgttat tgtgaaaagt aaaaaccgat
    11821 cacaaacagt atcgaattgg ttgaaatggt ttgatatgga cattgtagaa gagaacgacc
    11881 attacacaga tttgatttta aaaaatgatg atatttattt tagaattgaa gatggtaaag
    11941 tttcaaaata tcattcggtt ataataaaag acgcacaagc aacttcacca tattcaattt
    12001 ttatcagagg tgctatttat cgctttgaac cattagtata aatatacgta agtgctatga
    12061 gcgagaatgc ccatatgaat aatggcaagc gcaatggaaa gaatcgttaa tatattattt
    12121 aatcgtgatg acttaattaa aatgaaaaag attgataata taaatgtgaa aaagataagt
    12181 ataacccgta aactaaagta attcacggtg agaggttgac tcaatgtcat aatgattgca
    12241 acgatgttca taattataaa tagacttaaa ataattgttc tcatatcaaa cacctcattg
    12301 ttagattatt gacattataa caggggtaat tgtatatgaa cattaatgtg gttgcttgag
    12361 gagaaattta ttcattgaag tcaaattggt tcatgttaga aatgaatatc gtgttagatg
    12421 atgaaagtat attgaagtat aggtaactag ttgaaaagta ttaattgtac gataacatta
    12481 aatttaacag gaagcataga tataaaatga ttcacaatta aaatgagtaa atttgaactt
    12541 gctaaactat taattggagc atggacattt caaaaataag agttcaaatc ttacacaagc
    12601 tctgaatcga cactataaga tacaaactgt ataattaaag gtattgttaa atagaaggag
    12661 atatcataaa tcatggaaaa gatgcatatc actaatcagg aacatgacgc atttgttaaa
    12721 tctaacccaa atggagattt attacaatta acgaaatggg cagaaacaaa aaaattaact
    12781 ggatggtacg cgcgaagaat cgctgtaggt cgtgacggtg aaattcaggg tgttgcgcag
    12841 ttacttttta aaaaagtacc taaattacct tatacgctat gttatatttc acgtggtttt
    12901 gttgttgatt atagtaataa agaggcgtta aatgcattgt tagacagtgc aaaagaaatt
    12961 gctaaagctg agaaagcgta tgcaattaaa atcgatcctg atgttgaagt tgataaaggt
    13021 acagatgctt tgcaaaattt gaaagcgctt ggttttaaac ataaaggatt taaagaaggt
    13081 ttatcaaaag actacatcca accacgtatg actatgatta caccaattga taaaaatgat
    13141 gatgagttat taaatagctt cgaacgccga aatcgttcaa aagtgcgctt ggctttaaaa
    13201 cgaggtacga cagtagaacg atctgataga gaaggtttaa aaacatttgc tgaattaatg
    13261 aaaatcactg gggaacgcga tggcttctta acgcgtgata ttagttactt tgaaaatatt
    13321 tatgatgcgt tgcatgaaga tggagatgct gaactatttt tagtaaagtt ggatccaaaa
    13381 gaaaatatag cgaaagtaaa tcaagaattg aatgaacttc atgccgaaat tgctaaatgg
    13441 cagcagaaga tggaaacatc tgaaaagcaa gctaaaaaag cgcaaaatat gattaatgat
    13501 gcgcaaaata aaattgctaa aaatgaagat ttaaaacgag acctagaagc tttagaaaag
    13561 gaacatcctg aaggtattta tctttctggt gcactattaa tgtttgctgg ctcaaaatca
    13621 tattacttat atggtgcgtc ttctaatgaa tttagagatt ttttaccaaa tcatcatatg
    13681 cagtatacga tgatgaagta tgcacgtgaa catggtgcaa caacttacga tttcggtggt
    13741 acagataatg atccagataa agactcagaa cattatggat tatgggcatt taaaaaagtg
    13801 tggggaacat acttaagtga aaagattggt gaatttgatt atgtattgaa tcagccattg
    13861 taccaattaa ttgagcaagt taaaccgcgt ttaacaaaag ctaaaattaa aatatctcgt
    13921 aaattaaaac gaaaatagat taacgactga aatctgaacg ctcataagac tgtcatttgc
    13981 gttcagattt ttttacacaa tatagaatgg ttgagtaaaa tatttttgaa tatagtgaaa
    14041 gagggggaag tactgtgata aaaaagctat tacaattttc tttggggaat aagtttgcta
    14101 tctttttaat ggttgtttta gttgtcttgg gcggtgtata cgcgagtgct aaattgaaat
    14161 tagaattact accaaatgta caaaatccag ttatttcagt tacaacatca atgccgggtg
    14221 caacgccaca aagtacccaa gatgaaataa gtagtaaaat tgacaatcaa gtaagatcgt
    14281 tggcatatgt gaaaaatgtt aaaacgcaat ccatacaaaa tgcttcaatt gtaacagttg
    14341 aatatgaaaa taatacagat atggataaag cagaagaaca gcttaaaaaa gaaatcgata
    14401 aaattaaatt taaagatgaa gttggtcaac cagaattaag acgtaattcg atggacgctt
    14461 ttccggtttt agcatattca ttttcaaata aagagaatga cttgaaaaaa gtaacgaaag
    14521 tactgaatga acaattaata ccaaaattac aaacggtaga tggtgtgcaa aatgcgcaat
    14581 taaatgggca gacgaaccgt gaaatcaccc ttaaatttaa gcaaaatgaa cttgaaaaat
    14641 atgggttgac tgctgatgat gtagaaaact atctaaaaac ggcaacaaga acaacgccac
    14701 ttggattgtt ccaatttggt gataaagata aatcaattgt tgttgatggt caatatcaat
    14761 ctgttgatgc ttttaaaaac ataaatattc cattaacgtt ggcaggaggg caagggcaat
    14821 ctcaatccca aagtgacaat aaacaaaatt cagccatgtc agacgttaat caggcatcac
    14881 cgcagcaaaa ttcaaaaacg tcagcatcaa ataatataag tggtatgccg acagcgaaac
    14941 taggagattt agctgatatt acagttggtg atgtgcgtac ttctatttct aaaacgaatg
    15001 gcaaggatgc ggttaatcta caaataacta aagctcaaga tgccaataca gttcaagtag
    15061 ccaaagatgt acagcgtaaa attgatacat tcgttgatga aaataaagat tttaatgtca
    15121 caaaaacaat ggatactgca aagcctgttg agaaatcact ttatacgatg gttgaaaaag
    15181 catcattagg tacaatcgtg gcaattatag ttattttgct gtttttaaga aacattcgta
    15241 cgacggcaat ttctattata tccattccat tatcacttct tatggcgctt attgctctga
    15301 aattgagtga tgtttcattg aatatactaa cgttaggtgc attaacagta gcgattggac
    15361 gtgtgataga cgattcgatt gtagttgttg aaaatattta tcgacgctta acagattcag
    15421 aagaacaact aaaaggtgaa aatttaatta tcagtgcgac aactgaagta tttaaaccaa
    15481 taatgtcatc gacactagtt actattatcg tcttcttacc acttgtgttt gtatcaggtt
    15541 cagtaggaga aatgtttaga ccttttgcat tggctattgc atttagttta ttagcatcgt
    15601 tattagtgtc aattacactc gttccagcgt tggcagcaac actatttaaa aaaggcgtta
    15661 aacgtcgtaa taaacaacat caagaaggat taggtgttgt tagtacaact tataaaaaag
    15721 tattgcattg gtcactaaat cataagtgga ttgtaattat attaagtaca ttaattttgg
    15781 ttacaactat tgtatttgga ggaccgagac taggcactag ctttatttca gcaggtgacg
    15841 ataaattttt agctattact tatacaccga agcctggtga aacggagcaa gcagtgttga
    15901 atcatgcgaa agatgttgaa aaatatttaa aacagaaaaa gcatgtaaaa acaattcaat
    15961 actcagttgg cggtagtagt ccagtagatc caacgggtag tacaaatagt atggcaatca
    16021 tggttgaata tgataatgac acgcctaatt ttgatgtaga agcggataag gttattaaac
    16081 atgcagatgg ctttaaacat cctggagagt ggaaaaatca agatttagga acaggtgcag
    16141 gtaataaatc tgtagaggtt actgtgaaag gtccatctat ggatgccata aaatcaactg
    16201 taaaagatat tgaacagaaa atgaaacagg ttaaaggtct agccaatgtc aaatctgatt
    16261 tatcacaaac atatgatcag tatgaaatta aaattgatca aaataaagcg gcagaaaatg
    16321 gtatttctgc aagtcaactt gcaatgcact tgaatgaaaa cttaccaaaa aaaacagtta
    16381 cgactgttaa agaaaatggt aaaactgttg atgttaaagt caaacaaaat aagcaaacag
    16441 actggtcaga agataagttg aataatataa ctttgaaaaa gccgactggt ggtacgatta
    16501 aattgggaga tatcgctacg ttagttaaaa caacgacacc aagtaaattg acgcaagaac
    16561 aaggtgatta tgcaacgacg gtatctgcta aagtaacaaa taaagatgtg ggtggcacaa
    16621 cacgacaagt gatgtctaaa ataaataatt tggacaaacc gaataatgta aaggttaata
    16681 tcggtggtgc atcagatgat attaacaatg caatgacaca attagccttt gcaatgttag
    16741 ctgcaatcat tatcgtatat ttaatcctag ttattacatt taaaggtggc ttagcaccat
    16801 ttacaatttt attctcttta ccatttacag ttatcggtgt aattattgca ctattaatca
    16861 caggagaaac aatatcagta ccaagtttaa ttggtatgct aatgttaatt ggaattgtag
    16921 taacaaatgc cattgtgtta atagaccgtg tcattaataa tgagcagcag ggcatggaga
    16981 tgaaagaagc attaatcgaa gcaggcggta ctagaattag accgatatta atgacggcga
    17041 ttgcaacaat cggtgcatta gttcctttgt tatttggtca agatagctcg attcttattt
    17101 cgaaaggatt agccgctaca gttattggtg gtttaatttc atcaacttta ttaacacttg
    17161 tagttgtacc agtaatatat gaaattctct ttactttaaa aaaacgattc actaaacgat
    17221 agataatata aaagccataa aagcggtcat gatgttgact acttttatgg ctttttaaaa
    17281 tttgaaattt tagaccttat ttcacgtgcg atttaatcga tgtgttaata aaacaatact
    17341 ggcattcgat atgtaatctg atgataatat aaggttgcaa ttatgttatt gcatgttttg
    17401 tttattactt agaatagtac gatggttaag atgaaatata tgacgaagat aaataatgac
    17461 attaaattca agatgtttac gccttttaga tcttttttaa gcattttaaa tcctgtcact
    17521 tcgacattac caactaagag taataagacg ataaataccc accaattctc ttttggaaaa
    17581 ctcatcgtcg ttaatgctaa gacgaataat aacaacacac ccataataat tctcaaaatt
    17641 cgagtaaata caacttccat tttaaaaata ctaataaaac taacaattag ataaagtatt
    17701 gctagtaaaa tcagataaaa ttgcatatga taacttcctc tccgtttcaa agggttcatg
    17761 tagtacagta gcgtagaaaa cataaatagc agtgttggga tagatatgcg cgtatgttgt
    17821 taatgagaca ctaaatcata acacgacaac atcttgtgat tctagatgta attgagcgaa
    17881 aaatgaagca attcatcaat ataacgtatt cagagcaatg ctagtcaaat ttatgtaatg
    17941 aaaagcattt attgtctgaa tgatagatta ttaatcttgc atttgagcac gaacgtttct
    18001 aatttcattt tcaatatctt taagttgacg aacaagagcg gaagtatcta aattcaaaga
    18061 ttcccgtttt tctaagtcac cttgaagacg aacataatca tatttaagtt cggctagttg
    18121 gttttctaaa gacatactat aacactcctt tgaaatataa ttaagtgcat ttaatgtaga
    18181 ttatagcatg aaacagtaga cttctaaaat atctgtggag tatgttaaag ttataaatta
    18241 tgaagattat tgaaagaagg gataacagtg acagaacaat ttactatgat tattttatta
    18301 attgcgcttg gttacttttt gaagcgcatt aattatataa aggcaacaga tagtcaagtc
    18361 attgctacat tagttcttaa tgtcacactc ccatctttag ttattgtgaa tttaaacagt
    18421 gcagaattaa aattgtcatt atccatttta ccaattttaa tgattatcta tggtattgtg
    18481 gcgaaaatga ttgctgtctg gttttttaga aaatatgata atcacatgcg tggatcagta
    18541 ggtatgatga ctggtgctat gaatattgga ttgtttgcat atccactaat tgaagtgata
    18601 tggcctaaaa cgggacttat ttattttggt atggctgata taggtggtgc ctttgtgatg
    18661 tttggaatta cttactttgt aggtagctac tttagtgaag gtagtgatca attcgatttt
    18721 aaatatttag gtaagaaatt attacaatca gtgccactcg ttacatacat tgtaatgttt
    18781 atattgaata taagtcacat ccatttacca catgtggcaa tcgatttctt tagcatactt
    18841 tcccatgcaa atatgccgtt atcaatgatt ttgctaggtg tcatgttgaa ttttactctg
    18901 gaaagaaagt atatacctgc tactattaaa tatttatgtt tacattatgg attagcatta
    18961 gttgcgggat tgttagtaca tgtgttgcta ccggtatcag atgatatgat taagacgaca
    19021 ttgttggtca cttggatgtt cccggttggt gtcgcaatta tttcttatgg tattcagttt
    19081 aaatatcgaa cactgccatt tataggtatg acgaccaatt taacaatcat tattagtatc
    19141 attattttgt acgtgtatca ggcagtgttt gtctagttta cacatttgaa aataaaatcc
    19201 attttagtat tcgtagaaaa tgagagcccc ctttaccaca atatgtaaat gcatagtgtg
    19261 aaaagggggc ttttatttat gacatagttg ttcaactcat acattctagc agataatgca
    19321 gtgcgttatg cagctatcca cttcatatgt atggcgcata cctgataaac gaatatcatc
    19381 aagactttaa aagtatcgct aattttaaag tcttaaaccg catgattaat gtcatgatgt
    19441 gttattgcgt tttgttttta gagccatgta tttcctggga catatagagg aacattcaac
    19501 gtaaaattca cgaaaattaa ttttaaacat tctaaaagtg aatggttatt ttcagaaacg
    19561 ataacatttt tacgttacat gagtatttaa ctttctattt caaattacct agaattatag
    19621 cagctatcac gataattata atacctaccc aaataccagt catctgacga cgatcttttc
    19681 tttcgcctaa aatgaaaata ccgcctaatg ttgaaacgat aacaagtaac tgtgataatg
    19741 agaaacttgt agctacacca acttttggtt gagaatagaa catgaacaag ttaccaattc
    19801 cccaaatcac acctggcaat agattaagtg ctgttgattt aagtgatgtt ttatgattca
    19861 tggataggat aaagccacca attgccatac cgacagattg gaagaacaat gcatcagttc
    19921 caccaacacc aaagatgtca ccaagtacaa cgaaacctac atatccaact gtagatacaa
    19981 ttaaaatacc cattgctttt ttaaattcag gattatctga ttgacgttca tttttagctt
    20041 taagtgaagt aagtgctaca ccagtaacta ataatatcat ggcgattaaa ccaaagataa
    20101 tttgagtcat tgaagaccat tcacctaaaa agataacact gaataatgtt gtaccaacta
    20161 attgcatacc tgtagaaact ggcatagtat ttgatacacc gattaaacta atagatttaa
    20221 gctgattcgc ttgtccaaac gcccataatg caccagaaat aagaccgaca ataattacag
    20281 taggattatt gaattttgcg aaaccagtaa taagtaaacc taatccaaca atgagtgcgc
    20341 ctagcgtcgt accacgaatt tggttgtaag gtccaccgcc gacgaacaca ttaataagaa
    20401 cgacacttcc ccagaataaa gcaggtaaaa gtgcgattaa gaaatcaaga aattgcaaga
    20461 ctactcactc tcctatacta tttatgcaag tgctcccctt aattgacact tgcatgttat
    20521 tgtaaagcat tctgccctaa tagtaaaaaa caactactta taattttcaa tatttacaaa
    20581 aagtagaaaa acccacaata catgctaatg atgtactgtg gggtatttgc ttggtatttt
    20641 attcagggat tgtttgatct tttacgttac gtgattcgaa tttgaattta atgtactcat
    20701 agttgatatg gaaacctaga ccattaaatc gttcgatgag tttattattt ttgttacgaa
    20761 ttttaaaata aacttttcta atatcaaagt tatcaaaagc atattgcaat gcatacgcaa
    20821 gcaactcgaa agcgatacct ttcaaacggt aatctacatg agaactgaag tatttaatct
    20881 cggcgattga ctgttgtgaa tcaatttcaa ggtataaata acctttaagt aaaccttcgc
    20941 taacaaataa aaacaaatga tgatggtcgt ctaaactatc aatgatatct tgtggtgact
    21001 gggcctgata tttaaatgta gttgtgtgta atttgctgaa agcacgataa aaccctttgt
    21061 gataaggaat gatatttggt tggtgcatat cttcttctaa tcttgtacgg gcttctatgt
    21121 aatagtcagt gaagttataa cttgcttgaa taactttcat taatggtttg tattgttgaa
    21181 tgccttcttc aaaagaaaag ttaaagacgg catcgtcagg ttgattcgaa gtcattgttt
    21241 taaacagcgt ttcaaacgtt tcgacagata atacataatt gtcagccacg aaaggaccta
    21301 tgactttgaa tttattttta tcgtatgcaa aagacaatat gattgcttta attgaatcgt
    21361 tttctttaat tgaaaataca ccaggtgatt caatcgcatc ggttattaat acatctattt
    21421 cattgtactg ttgaggaagt ttatataagt atgatgctga ttcataattc gaatgtttaa
    21481 tgaacgattt tatttgtgta atatcatata actgaactat gctcatgtca ttacctccgt
    21541 actttttgtt acttttatta tatagtattt caactgaaat gaaagttaat agtgatatta
    21601 acatgttaca atacatttaa cacatcattt gcaatcattg aatttaaatc aaagattagt
    21661 ggaatagatg aagcacgttc gaaataaaag aaagtatgag aaaggataat ttatgaaatc
    21721 tttaatatta gctgaaaaac catcagtcgc aagagatatt gctgatgctt tacaaataaa
    21781 tcaaaagcgt aatggttact ttgaaaataa ccaatatatt gtcacgtggg cgttaggtca
    21841 tctagtgaca aatgcgacac ctgaacaata cgataaaaat ttaaaggaat ggcgattaga
    21901 agaccttcca attataccta aatatatgaa aactgttgtt attggtaaaa caagcaaaca
    21961 atttaaaaca gtaaaagcgt taattttaga taataaagtg aaagatatta ttattgcaac
    22021 agatgctgga cgagaaggtg aactagttgc aagattgatt ttggataaag ttggtaacaa
    22081 aaagccactc cgtcgattat ggattagctc agttactaaa aaagctattc aacaaggttt
    22141 taaaaattta aaagacggtc gccaatataa cgatttgtat tatgcagcgt tagcgagaag
    22201 tgaagcggat tggattgttg ggattaatgc aacgcgtgca ctaacaacaa agtatgatgc
    22261 acagctatcc ctgggacgtg ttcagacacc aacgattcaa ttagtaaata cacgacaaca
    22321 agagattaat cagttcaaac cacaacaata ctatacatta tcattaacgg taaaagggtt
    22381 tgattttcag ctagaatcaa atcagcgata taccaataaa gaaactttag aacagatagt
    22441 taataatttg aaaaatgtcg atggtaagat taaatctgtt gctactaaac ataagaagtc
    22501 gtatccgcaa tcactgtaca atttaacaga tttacaacaa gatatgtata gacgttataa
    22561 aattggacct aaagaaacat tgaatacact tcaaagttta tatgagagac ataaagtcgt
    22621 aacctatcca agaacagatt caaactattt aacaactgat atggtagata ctatgaaaga
    22681 acgtattcag gcgacgatgg caacaacata taaagaccaa gcgcgcccat taatgtctaa
    22741 aacattttca tcaaaaatgt cgatatttaa taatcaaaaa gtatctgatc accatgcaat
    22801 tattcctaca gaagtgagac ctgtcatgtc agacttaagt aatagagaat taaagttata
    22861 cgatatgatt gtcgagcgtt ttttagaagc tttaatgtct ccgcacgagt atgacgcgat
    22921 aactgttact ttagaggttg cagggcacac atttgttttg aaagagaatg taacaactgt
    22981 attaggcttt aaatctatta gacaaggtga atctattaca gagatgcaac agcctttttc
    23041 agaaggcgat gaagtgaaga tttcaaaaac aaacattaga gaacatgaaa caacacctcc
    23101 agaatatttt aatgaaggtt cgttattaaa agcgatggag aaccctcaga actttattca
    23161 attgaaggat aaaaaatatg cgcaaacatt aaaacaaaca ggtggtatcg gcacagttgc
    23221 aacaagggcc gacattatcg ataaattatt taatatgaat gccattgaat caagagacgg
    23281 taaaattaaa gtgacgtcaa aaggtaaaca aatattagaa ttagcaccag aagaattaac
    23341 gtcgccactt ttaactgcac aatgggaaga aaaattactt ttaattgaac gtggtaaata
    23401 tcaggcgaaa acattcatta atgaaatgaa agattttacg aaagatgttg taaatgggat
    23461 aaaaaatagt gatcgtaaat ataaacacga taatttaaca accactgaat gcccaacgtg
    23521 tggtaaattc atgattaaag ttaaaactaa aaatggtcag atgcttgtgt gccaagatcc
    23581 atcttgtaag acgaaaaaga atgtacagcg caaaacaaat gcaagatgtc caaactgtaa
    23641 aaagaaatta acgttgtttg gtaaaggaaa agaagcggta tatcgttgtg tttgtggaca
    23701 ttctgaaacg caagcacata tggatcagcg tatgaagtct aaatcctctg gtaaagtatc
    23761 tcgtaaagaa atgaaaaagt atatgaataa aaatgaaggt ttagacaata atccgtttaa
    23821 agatgcatta aaaaacttga atttatagat aaaatcgaac aaagttgaat cataaaatcg
    23881 aaaagttcgc ttttggtatt gttttttatt aagaatgata ttaaactatt aaggtatttt
    23941 aaaaaaagga gcatccattc gtgaaaaact atttccagtt cgataaatat ggaacaaact
    24001 ttaaaagaga aatattaggc ggtatcacaa ctttcttatc tatggcctat attttagcag
    24061 ttaacccgca agttttaagt ttagcaggtg ttaaaggcgt atcagaagat atgaaaatgg
    24121 accaaggtgc catttttgta gcgactgcat tagcagcatt tgtaggctcg ctattcatgg
    24181 ggctaatagc taaatatcca attgcgttag caccaggtat gggattaaat gcattctttg
    24241 catttactgt tgtgttaacc atgggtattc cttggcaagt tggtttaaca ggtgttttat
    24301 tctcaggtat attctttgcg atattaactg tcacagggtt tagggaagtt attattaatg
    24361 caattcctta tcaaatgaag atggctgttt cagcaggtat agggttattc attacatttg
    24421 taggtttaca aagttcgggt attatagtta aaaatgaatc tacattagta acattgggac
    24481 atttaactga tccaccagta cttttagcga tattcggtat cgttattaca gttattttat
    24541 atgcaattaa attgccaggt tctatcttta tcggtatgat catcacagca atcgttggta
    24601 tgttcactgg attgattcaa atgccatctg gtattgtagg taagattcca agtattgaac
    24661 caacgttcgg cgctgcattt gaagcgttta aagacccaag tcaattatta acgatacagt
    24721 tcttaattgt tattttaacg ttcttattta ttgatttctt tgatacagct ggtacattag
    24781 tagccgttgc tactcaagca ggtattatga aagataataa attaccaaga gctggtagag
    24841 cattgttctc agattcttta gctacgattg taggatcagt ttttggaaca actacaacaa
    24901 cttcttatat cgaatcaact tcaggtgttg ctgtaggtgc tagaacagga tttgctagta
    24961 tcgtaacagg ttgttgtttc ctattagcgc tattctttag tccgttaatc gctgttgtaa
    25021 caagtgcagt tacgacacca gctttagtgg tggtaggtgt attaatggct gcaaactttg
    25081 ctgaaataaa ctggaaaagt tttgaagttg cagtgccagc attcattaca atcattatga
    25141 tgcctttatc atattcaatt gcaactggta ttgcgtgtgg atttattttc tatccaatta
    25201 caatgcttat ctctaaaaaa cataaagaag tacatccgat aatgtatgta ttaatggtac
    25261 tattcatttt gtacttcata tttgttcatg gttaactcca gacacaaaaa acgactgcca
    25321 atgattggta gtcgtttttt ctttgaagta aggataaaat aagaagcgaa ataacataaa
    25381 gcaatattat gaatgtcttt aaatatccga tataaatata cttcttttaa aattgttgaa
    25441 cgtgtttctt ctataatata tatacctaat taaaaaggtt ttaagaatta ttctctatgt
    25501 ccatctttgt attgtgtgta aatgacacga gcatttttat taggtatata ggttggcgct
    25561 gataatacaa ctaggacaaa tagaatcatt aggaaaaata aaatccaatg aatcaaaatt
    25621 cctaagaagg ggataatagc taaaaaacta cctactatac caaatattgg aataatcgct
    25681 ataggtctaa ttgcattttg tctattcact aataatataa tagtaagaat tagataaagt
    25741 aatgcattaa agagtaaagg ctgccatcca aaacttaaaa taaacgttgc acctaaaaaa
    25801 ggaatgccat agaaaaactc acaaagtatt aaaaagatac ctataagtgc taaagataat
    25861 tttggtccat ttttcatatt cattcaccac cgttcttatg actaattata tagaataatt
    25921 ttcaatgata ataataaaat gattcaaggt acgaatttac aataaagaag gaaataaaaa
    25981 atatctcgaa aatagttgaa ctgactaagt atataaagta aaatataaaa gtatgtgtta
    26041 gacagataga aaaattatgt gaaaagcctt gatttatcga tgtttttcgt gtaatataac
    26101 taacgttgga ctttaagaaa agcgatgaag cgaaaggtta ctgacacacc cggccgcttt
    26161 gccatggcgc tgtgtaagat agttttcgtg gagaagtcta tcactaaatg tagacgaata
    26221 aggagggaaa attatggcaa aacaaaaaat cagaatcaga ttaaaagctt atgatcaccg
    26281 cgtgattgat caatcagcag agaagattgt agaaacagcg aaacgttctg gtgcagatgt
    26341 ttctggacca attccgttac caactgagaa atcggtatac acaatcatcc gtgccgtgca
    26401 taagtataaa gattcacgtg aacaattcga acaacgtaca cataaacgtt taatcgatat
    26461 tgtaaaccca acaccaaaaa cagttgacgc tttaatgggc ttaaacttac catctggtgt
    26521 agacatcgaa atcaaattat aatagacaat tttaggaggt ggactttcga tgaccaaagg
    26581 aatcttagga agaaaaattg ggatgacaca agtattcgga gaaaacggtg aattaatccc
    26641 tgtaacagta gtagaagcta aagaaaatgt tgtattacaa aagaaaactg tagaagttga
    26701 tggatacaac gcaatccaag ttggatttga agacaaaaaa gcatacaaaa aagatgcaaa
    26761 atctaacaaa tatgctaata aaccagctga aggtcacgct aaaaaagctg acgcagcacc
    26821 taagcgcttc attcgtgaat tccgcaatgt agacgtggat gcttacgaag taggtcaaga
    26881 agtctcagta gatacttttg tagctggcga cgttattgac gtaacaggcg tatcaaaagg
    26941 taaaggtttc caaggtgcaa ttaaacgcca cggacaatct cgtggaccta tgtcacacgg
    27001 ttctcatttc cacagagcac caggttctgt aggtatggct tcagatgctt ctagagtatt
    27061 caaaggccaa aaaatgccag gacgtatggg tggaaacact gtaactgttc aaaacttaga
    27121 agtagttcaa gttgacacag aaaacaaagt tatcttagta aaaggtaatg tacctggacc
    27181 taaaaaaggt ttagtagaaa tcagaacttc aattaaaaaa ggtaataaat aataagaagt
    27241 gaaaggagga aatgcataat ggctaattat gatgttttaa aattagacgg aactaaatca
    27301 ggttcaatcg aattaagcga tgcagtattc ggtattgagc caaataatag cgttttattc
    27361 gaagctatta atttacaacg tgcttcatta cgtcaaggta cgcatgctgt taagaatcgt
    27421 tcagcagtaa gcggtggcgg acgtaaacca tggaagcaaa aaggaacagg tcgtgctcgt
    27481 caaggtacaa tccgtgctcc acaatggcgt ggcggtggta tcgtattcgg accaactcca
    27541 agaagttatg catacaaaat gcctaagaaa atgcgtcgtt tagctttacg ctcagcatta
    27601 tctttcaaag ttcaagagaa tggcttaact gtagttgacg cattcaactt cgaagctcca
    27661 aaaactaaag aattcaaaaa tgtattatct acattagaac aacctaaaaa agtattagta
    27721 gttactgaaa acgaagatgt aaatgttgaa ttatcagcac gcaacatccc tggcgttcaa
    27781 gtgacaactg ctcaaggttt aaatgtttta gatatcacta atgctgacag cttagtaatt
    27841 actgaagctg ctgctaaaaa agttgaggag gtgctcggat aatggaagca agagatattc
    27901 ttaagcgccc cgtaatcact gagaaatctt ctgaagcaat ggctgaagac aaatacactt
    27961 tcgacgttga tactcgtgtt aacaaaacac aagtgaaaat ggcagttgaa gaaatcttca
    28021 acgtaaaagt tgcaagtgtt aatatcatga attacaaacc taagaaaaaa cgtatgggcc
    28081 gttaccaagg ctatacaaac aaaagaagaa aagcgattgt aactcttaaa gaaggatcaa
    28141 tcgacttatt taactaataa accaattacc attaaggagg taagcgacaa tggctattaa
    28201 aaagtataag ccaataacaa atggtcgtcg taatatgact tcgttagatt tcgcagaaat
    28261 cacgaaaact acacctgaaa agtcattatt aaaaccgcta ccgaaaaaag cgggacgtaa
    28321 caaccaaggt aaattgactg taagacacca tggtggtgga cacaaacgtc aataccgtgt
    28381 tatcgatttc aaacgtaaca aagatggtat caatgcaaaa gttgattcta ttcaatatga
    28441 tccaaaccgc tcagcaaaca tcgctttagt tgtatatgca gacggtgaaa aacgatatat
    28501 cattgctcct aaaggattag aagtaggtca aatcgttgaa agtggtgctg aagctgacat
    28561 caaagttggt aacgcattac cattacaaaa cattccagtt ggtacagtag tacacaacat
    28621 cgagcttaaa cctggtaaag gtggacaaat cgctcgttca gctggtgcaa gtgctcaagt
    28681 acttggtaaa gaaggtaaat acgtattaat cagattaaga tctggtgaag ttcgtatgat
    28741 cttatctact tgccgtgcta caatcggtca agttggtaac ctacaacacg aattagttaa
    28801 cgttggtaaa gccggacgtt caagatggaa aggtattcgt ccaacagttc gtggttctgt
    28861 aatgaaccct aacgatcacc cacacggtgg tggtgaaggt cgtgctccta tcggtagacc
    28921 atctccaatg tcaccatggg gtaaacctac gcttggtaag aaaactcgtc gtggtaaaaa
    28981 atcatcagac aaacttatcg ttcgtggacg taagaaaaaa taatataatc aacttatttg
    29041 ggtgtgcggc ttaaagctgc acgcacataa taagaaggga ggcgcccaaa tggctcgtag
    29101 tattaaaaaa ggacctttcg tcgatgagca tttaatgaaa aaagttgaag ctcaagaagg
    29161 aagcgaaaag aaacaagtaa tcaaaacatg gtcacgtcgt tctacaattt tccctaattt
    29221 catcggacat acttttgcag tatacgacgg acgtaaacac gtacctgtat atgtaactga
    29281 agatatggta ggtcataaat taggtgagtt tgctcctact cgtacattca aaggacacgt
    29341 tgcagacgac aagaaaacaa gaagataata tctattaagt agaggaggac atcctaatgg
    29401 aagcaaaagc ggttgctaga acaataagaa tcgcacctcg taaagtaaga ctagttcttg
    29461 acttaatcag aggtaaaaat gctgctgaag ctattgcaat tttaaaatta acaaacaaag
    29521 cttcatcacc agtaattgaa aaagtattaa tgtccgcttt agctaatgct gaacataact
    29581 atgacatgaa cacagatgaa ttagtagtta aagaagcata tgctaacgaa ggaccaacat
    29641 taaaacgttt ccgtccacgt gcgcaaggtc gtgcaagtgc gattaacaaa cgtacaagcc
    29701 acattacaat cgtcgtaagt gacggtaaag aagaagctaa agaagcttaa ttaactttta
    29761 aggagggaat actgtgggtc aaaaaattaa tccaatcgga cttcgtgttg gtattatccg
    29821 tgattgggaa gctaaatggt atgctgaaaa agacttcgct tcacttttac acgaagattt
    29881 aaaaatccgt aaatttattg ataatgaatt aaaagaagca tcagtttctc acgtagagat
    29941 tgaacgtgct gcaaaccgta tcaacattgc aattcatact ggtaaacctg gtatggtaat
    30001 tggtaaaggc ggttcagaaa tcgaaaaatt acgcaacaaa ttaaatgcgt taactgataa
    30061 aaaagtacac atcaacgtaa ttgaaatcaa aaaagttgat cttgacgctc gtttagtagc
    30121 tgaaaacatc gcacgtcaat tagaaaaccg tgcttcattc cgtcgtgtac aaaaacaagc
    30181 aatcactaga gctatgaaac ttggtgcaaa aggtatcaaa actcaagtat ctggtcgttt
    30241 aggcggagct gacatcgctc gtgctgaaca atattcagaa ggaactgttc cacttcatac
    30301 gttacgtgct gacatcgatt atgcacacgc tgaagctgac actacttacg gtaaattagg
    30361 cgttaaagta tggatctatc gtggagaagt tcttcctact aagaacacta gtggaggagg
    30421 aaaataataa tgttactacc aaaacgtgta aaatatcgtc gtcaacatcg tcctaaaaca
    30481 actggtcgtt ctaaaggcgg taactacgta acatttggtg agtttggttt acaagctaca
    30541 acaacgtctt ggatcacatc tcgtcaaatc gaatctgctc gtatagcaat gacacgttac
    30601 atgaaacgtg gcgggaaagt ttggattaaa atcttcccac atacaccata tactaaaaaa
    30661 cctttagaag tacgtatggg tgctggtaaa ggtgcggttg aaggctggat cgcagttgtt
    30721 aaaccaggta gaattttatt cgaagttgct ggcgtttctg aagaagttgc gcgtgaagca
    30781 ctacgtttag caagtcacaa acttccagta aaaactaagt ttgtaaaacg tgaggaattg
    30841 ggtggtgaaa caaatgaaag ctaaggaaat tagagactta accacttcag aaatcgaaga
    30901 acaaatcaaa tcttcaaaag aagagctttt taacctacgc tttcagttag ctacaggtca
    30961 attagaagaa actgcacgta ttcgtacagt aagaaaaacg attgcgcgtc taaaaactgt
    31021 tgctcgtgaa agagaaattg aacaaagtaa ggctaatcaa taattaagta agaggaggtt
    31081 acaaaagtga gcgaaagaaa cgatcgtaaa gtttatgtag gtaaagttgt ttcagacaaa
    31141 atggacaaga ctattacagt acttgttgaa acttacaaaa cacacaaatt atacggtaaa
    31201 cgagtgaaat actctaaaaa atacaaaact catgatgaaa acaattcagc taaattagga
    31261 gacattgtta aaattcaaga aactcgtcct ttatcagcaa caaaacgttt tcgtttagta
    31321 gagattgttg aagagtcagt aattatttaa tacaagttta gagataaggg aggtttaact
    31381 aatgatccaa caagaaacac gcttgaaagt agcagacaac tctggtgctc gtgaagttct
    31441 tacaatcaaa gtattaggtg gatctggtcg taaaacagca aacatcggcg atgttatcgt
    31501 atgtactgtt aaaaatgcaa caccaggtgg cgttgttaaa aaaggtgacg ttgtcaaagc
    31561 tgtaatcgta cgtactaagt caggtgttcg tcgtaatgac ggttcatata tcaaatttga
    31621 tgaaaatgca tgtgttatca tccgtgatga caaaggccca cgtggtactc gtatcttcgg
    31681 acctgttgct cgtgaattac gtgaaggtaa cttcatgaaa atcgtatcat tagcaccaga
    31741 agtactttaa ttaataacaa ccaatacaaa taaggaggtg cccacatgca tatcaaaaaa
    31801 ggtgacaacg ttaaagttat cgcaggtaaa gacaaaggta aagaaggtaa agtaatcgct
    31861 actctaccta aaaaagaccg tgtcgttgtg gaaggtgtta acattatgaa aaaacaccaa
    31921 aaaccaactc aattaaatcc tgaaggtgga atcttagaaa cagaggcagc aatccatgtt
    31981 tctaatgtac aattattgga ccctaaaaca aacgaaccaa ctcgtgtagg ttacaaattt
    32041 gttgatggta aaaaagttcg tatcgctaaa aaatctggcg aagaaattaa atctaataat
    32101 taataacaag ttgaaaggag gatcccactt tgaaccgttt aaaagaaaag tttaacactg
    32161 aagttactga aaacttaatg aaaaaattca attatagttc agtaatggaa gtaccaaaaa
    32221 tagataaaat cgttgtgaac atgggtgtag gtgacgcagt acaaaattct aaagtattag
    32281 acaatgctgt tgaagaatta gaattgatca ctggtcaaaa accattagta actaaagcta
    32341 aaaaatcaat cgcgactttc cgtttacgtg aaggtatgcc aatcggtgcg aaagtaacac
    32401 ttcgcggtga aagaatgtat gaattcttag acaaattaat ttcagtatca ttaccacgtg
    32461 tacgtgactt ccaaggtgtt tctaaaaaag catttgacgg acgcggtaac tacactttag
    32521 gtgttaaaga acaattaatt ttcccagaaa tcgactatga taaagtaagt aaagttagag
    32581 gaatggatat tgttatcgta acgactgcta acactgatga agaagctcgt gaattgttag
    32641 ctaacttcgg tatgccattc cgtaaataat tatttaaagg aggctaatta agtggctaaa
    32701 acttcaatgg ttgctaagca acaaaaaaaa caaaaatatg cagttcgtga atacactcgt
    32761 tgtgaacgtt gtggccgtcc acattctgta tatcgtaaat ttaaattatg ccgtatttgt
    32821 ttccgtgaat tagcttacaa aggccaaatc cctggcgttc gtaaagctag ctggtaataa
    32881 aaaagagtct gaaaggaggc aacaatcaat gacaatgaca gatccaatcg cagatatgct
    32941 tactcgtgta agaaacgcaa acatggtgcg tcacgagaag ttagaattac ctgcatcaaa
    33001 tattaaaaaa gaaattgctg aaatcttaaa gagtgaaggt ttcattaaaa atgttgaata
    33061 cgtagaagat gataaacaag gtgtacttcg tttattctta aaatatggtc aaaacgatga
    33121 gcgtgttatc acaggattaa aacgtatttc aaaaccaggt ttacgtgttt atgcaaaagc
    33181 tagcgaaatg cctaaagtat taaatggttt aggtattgca ttagtatcaa cttctgaagg
    33241 tgtaatcact gacaaagaag caagaaaacg taatgttggc ggagaaatta tcgcatacgt
    33301 ttggtaataa aaaataagga ggtgccataa catgagtcgt gttggtaaga aaattattga
    33361 catccctagt gacgtaacag taacttttga tggaaatcat gtaactgtta aaggtcctaa
    33421 aggtgaatta tcaagaactt taaatgaaag aatgacattc aaacaagaag aaaacacaat
    33481 tgaagttgta agaccatctg attctaaaga agatagaaca aaccatggta caactcgtgc
    33541 tttattaaat aatatggtac aaggtgtttc tcaaggatac gtaaaagtac ttgaacttgt
    33601 tggtgtaggt taccgtgctc aaatgcaagg taaagactta atccttaacg ttggttattc
    33661 tcacccagta gaaattaaag ctgaagaaaa cattactttc tcagttgaga aaaacacagt
    33721 cgttaaagtt gaaggtattt caaaagaaca agttggagca ttagcatcta acatccgttc
    33781 agtaagacct ccagagcctt acaaaggtaa aggtattcgt taccaaggtg aatacgttcg
    33841 ccgtaaagaa ggtaaaactg gtaaataata gataaactct aaagaaagga gtattgaaat
    33901 gatcagtaaa attgataaaa ataaagtgcg tttaaaaaga catgctcgtg ttcgtactaa
    33961 cttatcaggt acagctgaaa agccacgttt aaacgtatat cgttcaaaca agcatatcta
    34021 cgctcaaatt attgatgata ataaaggcgt aacattagct caagcttctt caaaagacag
    34081 cgacattgct actacagcaa ctaaagttga attagcaact aaagtcggtg aagcaattgc
    34141 taaaaaagct gctgacaaag gcattaaaga aatcgtattt gaccgtggag gatatttata
    34201 tcacggacgt gttaaagcat tagctgaagc agcaagagaa agcggattag aattttaatt
    34261 taaaggaggg acaaatacat ggctcgtaga gaagaagaga cgaaagaatt tgaagaacgc
    34321 gttgttacaa tcaaccgtgt agcaaaagtt gtaaaaggtg gtcgtcgttt ccgtttcact
    34381 gcattagttg tagttggaga caaaaatggt cgtgtaggtt tcggtactgg taaagctcaa
    34441 gaggtaccag aagcaatcaa aaaagctgtt gaagcagcta aaaaagattt agtagttgtt
    34501 ccacgtgttg aaggtacaac tccacacaca attactggcc gttacggttc aggaagcgta
    34561 tttatgaaac cggctgcacc tggtacagga gttatcgctg gtggtcctgt tcgtgccgta
    34621 cttgaattag caggtatcac tgatatctta agtaaatcat taggatcaaa cacaccaatc
    34681 aacatggttc gtgctacaat cgatggttta caaaacctta aaaatgctga agatgttgcg
    34741 aaattacgtg gcaaaacagt agaagaatta tacaattaag gagggaaaac tagttatggc
    34801 taaattacaa attaccctca ctcgtagtgt tattggtcgt cctgaaacac aacgtaaaac
    34861 tgttgaagct ttaggtctta aaaagactaa cagttcagta gttgttgaag ataaccctgc
    34921 tattcgtggg caaatcaaca aagttaagca cttagtaaca gtagaagaaa aataatcaag
    34981 gaggtgccga aatgaaatta catgagttaa aaccggcaga aggttcacgt aaagaacgca
    35041 atcgtgttgg acgtggtgtt gcgacaggta acggtaaaac aagtggtcgc ggacacaaag
    35101 gtcaaaaagc tcgttcaggc ggtggtgtaa gaccaggatt cgaaggtggt caattaccat
    35161 tattccgtcg tttaccaaaa cgtggtttta ctaacataaa tcgtaaagaa tatgctattg
    35221 ttaacttaga ccaacttaat aaatttgaag atggtactga agtaactcca gctttattag
    35281 tagaatctgg tgttgttaag aatgaaaaat ctggtatcaa aatactaggt aatggttcac
    35341 ttgataagaa attgacagtg aaagctcata aattctcagc ttcagcagca gaagctattg
    35401 atgctaaagg tggagcacac gaggtgatct aatgattcaa acccttgtga acttctttag
    35461 aacaaaagag gttcgtaaca agattttctt cacactagca atgttagtaa tttttaaaat
    35521 agggacttat ataccagctc caggagtaaa tcctgcagct tttgataatc cccaaggttc
    35581 tcaaggtgcc actgagttat taaatacttt tggtggcgga gccttgaaac gattttctat
    35641 ttttgcaatg ggtattgtac cctacatcac tgcatcaatc gtaatgcaat tattacaaat
    35701 ggatattgtc cctaaattct cagaatgggc aaaacaaggt gaagtaggta gaagaaagtt
    35761 aaataacgtt actcgttatt tagcaatttc tttagcattt atccaatcta taggtatggc
    35821 attccaattt aataattatc tcaaaggtgc gctgattatc aatcagtcaa ttatgagtta
    35881 tttattaata gcactagttt tgacagcagg aactgctttc ttaatatggc ttggtgatca
    35941 aatcactcag ttcggtgttg gtaatggtat ttctattatc atatttgcgg gtattttatc
    36001 aacgttgcca gccagtctaa ttcaatttgg acaaactgcg ttcgttgggc aagaagatac
    36061 atcattagca tggttgaaag tattaggact tttagtgtca ctgatactat taactgtagg
    36121 tgctatttat gtacttgaag ccgttcgtaa aattccgatt caatatgcta agaaacaaac
    36181 tgctcaaaga ttaggttcac aggcaactta tctaccttta aaagtgaact cagccggtgt
    36241 tattcctgta atctttgcaa tggcgttctt cttactgcct agaacattaa cgttgttcta
    36301 tccagataaa gaatgggcac aaaacattgc gaatgctgca aatccttcaa gtaatgttgg
    36361 tatggttgta tatattgtac taattatact attcacatac ttctatgcat ttgtacaagt
    36421 taatccggaa aaaatggctg ataaccttaa gaaacaaggt agctatgttc caggtattag
    36481 acctggtgaa caaaccaaaa aatatattac taaagtttta tatcgtttaa cttttgtagg
    36541 ttctatcttc ttagccgtta tttcaattct accgatatta gcaactaagt ttatgggatt
    36601 accacaatca attcagattg gtggtacaag tttacttatc gttattggtg tagcgattga
    36661 aacaatgaaa tctttagaag cacaagtatc tcaaaaagaa tataaaggct ttggtggtag
    36721 ataattttag gagggcactt atgaatatca ttttgatggg tttacctggc gcaggtaaag
    36781 gaactcaagc aagtgaaatt gtcaagaaat tcccaatacc ccacatttca actggtgaca
    36841 tgttcagaaa agctataaaa gaagaaactg aattaggtaa agaagctaag tcttatatgg
    36901 accgtggcga attagttcct gatgaagtga ctgtaggtat cgttaaggaa agaatttctg
    36961 aagacgatgc aaaaaaaggc tttttattag atggcttccc aagaacaatc gagcaagctg
    37021 aggcattaaa taatattatg tctgagcttg acagaaacat tgatgctgtc atcaatatcg
    37081 aagttccgga agaagaatta atgaaccgtc ttacaggtcg tcgaatctgt gagtcatgtg
    37141 gtacaacgta tcatcttgta tttaatcctc cgaaggtcga aggtatttgt gatatcgatg
    37201 gtggtaaatt gtatcaacga gaagatgata atcctgaaac ggtagctaat cgtttgagtg
    37261 ttaatattaa acaatctaaa cctattttag atttctatga tcaaaaaggt gtattgaaaa
    37321 atattgatgg ttcaaaagat attagcgacg ttaccaaaga tgtcattgat attttagatc
    37381 atttgtaata gatcaacttt tagcggtcta ttttaagtga ggcatgattt aatcatatta
    37441 gatagatgag agtggcaatt aacgataatt aattcccgta atttttgttt aacttataaa
    37501 attaatcact gatagattcg taacaataaa gttgtctatt gacgattacc ttactattgc
    37561 aaaaaggggg aagttaatca atggctaaac aagatgtaat tgaattagaa ggtactgtat
    37621 tagatacttt accgaacgca atgtttaaag tagaattaga aaatggtcat gagattttag
    37681 ctcacgtaag tggtaaaatc agaatgaatt acattcgtat tctacctggc gacaaagtaa
    37741 ctgttgagat gtctccgtac gatttaacac gcggaagaat tacttatcgt tataaataat
    37801 cgtcactcca taatataggg aggtataaaa atgaaagtaa gaccatcagt aaaacctatt
    37861 tgcgaaaaat gtaaagtcat taaacgtaaa ggtaaagtaa tggtaatttg tgaaaatcca
    37921 aaacacaaac aaagacaagg ttaataaaag agaggtgtaa attaatatgg cacgtattgc
    37981 aggagtagat attccacgtg aaaaacgcgt agttatctca ttaacttata tatacggtat
    38041 cggtacgtca actgctcaaa aaattcttga agaagctaac gtatcagctg atactcgtgt
    38101 gaaagattta actgatgacg aattaggtcg catccgtgaa gttgtagacg gttataaagt
    38161 cgaaggtgac ttacgtcgtg aaactaactt aaatatcaaa cgtttaatgg aaatttcatc
    38221 ataccgtggt atccgtcacc gtcgtggttt accagttcgt ggtcaaaaaa cgaaaaacaa
    38281 cgcgcgtact cgtaaaggac cagttaaaac ggtagctaac aagaaaaaat aataggtaaa
    38341 ggaggcaaat tttaaatggc acgtaaacaa gtatctcgta aacgtagagt gaaaaagaat
    38401 attgaaaatg gtgtagcaca catccgttca acattcaaca acactattgt aactatcact
    38461 gatgagttcg gtaatgcttt atcatggtca tcagctggtg cattaggatt caaaggatct
    38521 aaaaaatcaa caccatttgc agcacaaatg gcttctgaaa ctgcatctaa atcagctatg
    38581 gagcatggtt taaaaacagt tgaagtaaca gttaaaggac ctggtccagg tcgtgaatca
    38641 gctattcgtg cattacaatc tgcaggttta gaagtaactg cgatcagaga cgttactcca
    38701 gtacctcata acggttgtcg tccaccaaaa cgtcgtcgtg tataatttat gatggtattg
    38761 ttacaggtca ctgagcaaac attttaaatt aagtcgacgt atataaggag gatatttaaa
    38821 tgatagaaat cgaaaaacct agaattgaga caattgaaat tagtgaagat gctaaattcg
    38881 gtaagttcgt tgttgaacca ctagaacgtg gctacggtac tacactagga aactccttac
    38941 gtcgtatcct actatcttca ttaccaggtg cagccgttaa gtacattgaa attgagggag
    39001 ttttacatga attctcagca gtagacaatg tagttgaaga tgtttctaca attattatga
    39061 acattaaaca attagcattg aaaatttact ctgaagaaga taaaacttta gaaattgatg
    39121 tacgtgatga aggcgaagta acagcaagcg acattacaca tgacagtgat gttgaaattt
    39181 taaacccaga gcttaaaatt gcaacagtat ctaaaggtgg tcacttaaaa attcgtctag
    39241 ttgctaacaa gggtagaggt tacgcattag cagaacaaaa taatactagt gatttaccaa
    39301 ttggtgtaat ccctgttgat tcattgtatt cacctgttga acgtgtgaac tatactgttg
    39361 aaaatacacg tgtaggtcaa agcagtgatt ttgataaatt aacattggat gtttggacta
    39421 atggttcaat cacaccacaa gaatcagttt cattagcagc aaaaataatg actgaacact
    39481 tgaatatctt cgttggtctt actgatgaag cgcaaaacgc tgaaatcatg attgaaaaag
    39541 aagaagatca aaaagaaaaa gtattagaaa tgtctattga agaattagac ttatctgtac
    39601 gttcatataa ctgcttaaaa cgcgcaggaa tcaattctgt tcaagagtta gctgacaaat
    39661 ctgaagctga catgatgaaa gtgcgtaatt taggtcgtaa atctttagaa gaagttaaat
    39721 acaaattaga agatttagga ttaggattaa gaaaagaaga ttgataaagg aggttaactc
    39781 atgggttaca gaaaattagg tcgtacttct gatcaacgta aagctatgtt acgtgactta
    39841 gctacatcac ttattattag tgaacgtatt gaaactacag aagctcgtgc aaaagaagtt
    39901 cgcagtgttg ttgagaaatt aatcacttta ggtaaaaaag gagatttagc ttctcgtcgt
    39961 aatgcagcta aaactttacg taatgttgaa atcttaaacg aagatgaaac tacacaaact
    40021 gcacttcaaa aattatttgg tgaaatcgca gagcgttaca cagaacgtca aggtggttac
    40081 actcgtatcc ttaaacaagg ccctcgtcgc ggtgacggtg ctgaatcagt aattatcgaa
    40141 ttagtataag aatttatata aatacactaa ctacttttat ataaagcaaa tgagtgcaac
    40201 gataatgttt gccacataca aatattgtct agctcagagt accccatcaa ctaaataaag
    40261 atttaaagcg tgaactcaac aaacttatga tggggtgcgc gcttttttat tgaataagat
    40321 atcggactta tgaacagaaa t
//
LOCUS       DAKSQE010000011        40107 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.011, whole genome shotgun sequence.
ACCESSION   DAKSQE010000011 DAKSQE010000000
VERSION     DAKSQE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 40107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40107
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.011"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            374..1246
                     /locus_tag="O0V33_000447"
     CDS             374..1246
                     /locus_tag="O0V33_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCX8975132.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
     gene            complement(1629..3008)
                     /locus_tag="O0V33_000448"
     CDS             complement(1629..3008)
                     /locus_tag="O0V33_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCX8975133.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EEVEGHPFKMPGGAVSNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            3288..3644
                     /locus_tag="O0V33_000449"
     CDS             3288..3644
                     /locus_tag="O0V33_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975134.1"
                     /translation="MLKQILPRATKISVIFAVAFFIINYIGMEKPDTLYLVGRTIIAT
                     LAFILICLTVFSIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            3641..3826
                     /locus_tag="O0V33_000450"
     CDS             3641..3826
                     /locus_tag="O0V33_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975135.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(3922..4113)
                     /locus_tag="O0V33_000451"
     CDS             complement(3922..4113)
                     /locus_tag="O0V33_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975136.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILAIIGYFLLLQGKL"
     gene            complement(4124..4759)
                     /locus_tag="O0V33_000452"
     CDS             complement(4124..4759)
                     /locus_tag="O0V33_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCX8975137.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDEYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            complement(4834..5751)
                     /locus_tag="O0V33_000453"
                     /pseudo
     CDS             complement(4834..5751)
                     /locus_tag="O0V33_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            6019..6549
                     /locus_tag="O0V33_000454"
     CDS             6019..6549
                     /locus_tag="O0V33_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975138.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            complement(6615..8219)
                     /locus_tag="O0V33_000455"
     CDS             complement(6615..8219)
                     /locus_tag="O0V33_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCX8975139.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSSKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVVIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDNVDPKRASTGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVEDTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
     gene            8550..9314
                     /locus_tag="O0V33_000456"
     CDS             8550..9314
                     /locus_tag="O0V33_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCX8975140.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHVDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTEKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            complement(9372..9878)
                     /locus_tag="O0V33_000457"
     CDS             complement(9372..9878)
                     /locus_tag="O0V33_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCX8975141.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGNYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDSMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(10048..11358)
                     /locus_tag="O0V33_000458"
     CDS             complement(10048..11358)
                     /locus_tag="O0V33_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HCX8975142.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIILMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFSWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLVAILFNFPKLKKNTSQEMK"
     regulatory      complement(11435..11530)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11583..12152)
                     /locus_tag="O0V33_000459"
     CDS             complement(11583..12152)
                     /locus_tag="O0V33_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975143.1"
                     /translation="MALIVSRAILFLILALALGIATVFPEQKLPVYILFTLFVVVAIV
                     SNVWLTHQNVKKHIIKFDKHVVYFLGVLSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIRNTRPSNKQLKVISEQSAQQFKVISLILVSVSIVFIFIALMLKW
                     WLILAISIIVLIIITVAMIVYEKYYTTPN"
     gene            complement(12639..13520)
                     /locus_tag="O0V33_000460"
     CDS             complement(12639..13520)
                     /locus_tag="O0V33_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCX8975144.1"
                     /translation="MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGNQ
                     RLQGYKMLVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            complement(13631..14752)
                     /locus_tag="O0V33_000461"
                     /pseudo
     CDS             complement(13631..14752)
                     /locus_tag="O0V33_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
     gene            complement(15027..16265)
                     /locus_tag="O0V33_000462"
     CDS             complement(15027..16265)
                     /locus_tag="O0V33_000462"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HCX8975145.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEVIALLPEVKHYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTLYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNIQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTLSFKV"
     gene            complement(16265..17962)
                     /gene="hutU"
                     /locus_tag="O0V33_000463"
     CDS             complement(16265..17962)
                     /gene="hutU"
                     /locus_tag="O0V33_000463"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017547454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HCX8975146.1"
                     /translation="MELTSKKNRSEFYEKNSSKKGLSIECKGWEQEAVLRMLYNNLDT
                     EVAERPEDLVVYGGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEA
                     PRVLISNSVLVPEWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAEL
                     GNQHFNGDLAGTVTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIATKYCDI
                     KTANLDEALKLAEEAKERGEGLSIGLVGNAVDLHQAILEKGFKIDIITDQTSAHDPLN
                     GYVPQGYSVEEAKVLREKDPKKYVELSQASMAKHVELMLEFHKRGAVAFDYGNNIRQV
                     AFNNGVRNAFDFPGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENE
                     KLLRWLDLAEEKISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLD
                     AGSVASPNRETESMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGM
                     VVVADGSERAERRLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            18085..18969
                     /locus_tag="O0V33_000464"
     CDS             18085..18969
                     /locus_tag="O0V33_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCX8975147.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLCRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            19211..19630
                     /gene="fosB"
                     /locus_tag="O0V33_000465"
     CDS             19211..19630
                     /gene="fosB"
                     /locus_tag="O0V33_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB/FosD family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HCX8975148.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFKLAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
     gene            complement(19886..20821)
                     /locus_tag="O0V33_000466"
     CDS             complement(19886..20821)
                     /locus_tag="O0V33_000466"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HCX8975149.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSTPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFATLAAKSIVNHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTGYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFIHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            21099..22358
                     /gene="sdpC"
                     /locus_tag="O0V33_000467"
     CDS             21099..22358
                     /gene="sdpC"
                     /locus_tag="O0V33_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HCX8975150.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSVIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKIQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYDTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGIAKNDTENTVADEPSIHEGTEKTES
                     QHHIDNQTESNHDEDHDITSKSVESAESVKHAPQSDDLTNDSNEDETEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            complement(22424..23110)
                     /locus_tag="O0V33_000468"
     CDS             complement(22424..23110)
                     /locus_tag="O0V33_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HCX8975151.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKMVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            23281..23934
                     /locus_tag="O0V33_000469"
     CDS             23281..23934
                     /locus_tag="O0V33_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HCX8975152.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPSLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
     gene            complement(24170..25189)
                     /locus_tag="O0V33_000470"
     CDS             complement(24170..25189)
                     /locus_tag="O0V33_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCX8975153.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLQGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRGNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
     gene            complement(25219..25545)
                     /locus_tag="O0V33_000471"
     CDS             complement(25219..25545)
                     /locus_tag="O0V33_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCX8975154.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGVILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            complement(25594..25779)
                     /locus_tag="O0V33_000472"
     CDS             complement(25594..25779)
                     /locus_tag="O0V33_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975155.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            complement(25763..26995)
                     /locus_tag="O0V33_000473"
     CDS             complement(25763..26995)
                     /locus_tag="O0V33_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8975156.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGIIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGIMAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGRLALITSYIPFLAPFQLVVRAQTSGLQLHEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            complement(26988..27887)
                     /locus_tag="O0V33_000474"
     CDS             complement(26988..27887)
                     /locus_tag="O0V33_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8975157.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDMSLELESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTHIRGIIHSENMKQGLQLTIENEDVSKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            complement(28120..28767)
                     /locus_tag="O0V33_000475"
     CDS             complement(28120..28767)
                     /locus_tag="O0V33_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCX8975158.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMMLWHLILMV
                     PAVCVLIISLFIFFISIAADNEALIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVLLYFVYLGLTI
                     VMIVFLCFDSKPANKYGESPKYPSSIKNQSVTSETPKKAGTSETPKTPEETDNQL"
     gene            29166..29774
                     /locus_tag="O0V33_000476"
     CDS             29166..29774
                     /locus_tag="O0V33_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCX8975159.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEDG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            30110..31318
                     /gene="gltS"
                     /locus_tag="O0V33_000477"
     CDS             30110..31318
                     /gene="gltS"
                     /locus_tag="O0V33_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCX8975160.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTAQTGINIPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(31366..32415)
                     /locus_tag="O0V33_000478"
     CDS             complement(31366..32415)
                     /locus_tag="O0V33_000478"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCX8975161.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMTYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRSLNIHRG"
     gene            complement(32446..33393)
                     /locus_tag="O0V33_000479"
     CDS             complement(32446..33393)
                     /locus_tag="O0V33_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCX8975162.1"
                     /translation="MALTIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFSFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYRYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            33490..33981
                     /locus_tag="O0V33_000480"
     CDS             33490..33981
                     /locus_tag="O0V33_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCX8975163.1"
                     /translation="MVIITQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQPTITWPEQLGHKIAIPHKG
                     ALK"
     gene            33978..34397
                     /locus_tag="O0V33_000481"
     CDS             33978..34397
                     /locus_tag="O0V33_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975164.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(34505..35425)
                     /locus_tag="O0V33_000482"
     CDS             complement(34505..35425)
                     /locus_tag="O0V33_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCX8975165.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGHQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNN"
     gene            35633..36271
                     /locus_tag="O0V33_000483"
     CDS             35633..36271
                     /locus_tag="O0V33_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975166.1"
                     /translation="MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVNFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADALKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            complement(36517..38454)
                     /locus_tag="O0V33_000484"
     CDS             complement(36517..38454)
                     /locus_tag="O0V33_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCX8975167.1"
                     /translation="MTTTFIISYIVLALIIVGVINLLLIRSRRKAKRQQKEQHFATRQ
                     SNQSKFKASDLDRTTDQSSQHTAREEARIDNKDNQNQVSLNKQTEGSEQDQASFTDNN
                     ESDEEVYAAKNPNSEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTL
                     LNVALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFT
                     IGSLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMI
                     LAPAIGPTLSGYIVQNYHWNVMFYGMFIIGILAILVGFVWFKLYQYTTNPKADIPGII
                     FSTIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKAPMLNLEVLK
                     FPTFTLTTVINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFA
                     GKLLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMV
                     TAAINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNP
                     VVQDHMRELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQ
                     SNKKAKATAQKIDADNSINHE"
     gene            complement(38467..39114)
                     /locus_tag="O0V33_000485"
     CDS             complement(38467..39114)
                     /locus_tag="O0V33_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCX8975168.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYITTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVATVTVQGQDGETKDMELKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASVKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLDSEPSKQVVPGMNAEVKIHKN"
     gene            39235..39789
                     /locus_tag="O0V33_000486"
     CDS             39235..39789
                     /locus_tag="O0V33_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCX8975169.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKRNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQKIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQNQ"
     gene            complement(39913..40107)
                     /locus_tag="O0V33_000487"
                     /pseudo
     CDS             complement(39913..40107)
                     /locus_tag="O0V33_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT        13373 a         7543 c         5635 g        13556 t
ORIGIN      
        1 taaaaagttt cttcaaattg taaagatgca ttaatcgagt tataatcata atgattaaga
       61 tggtagattt agtcttatat aactacattt actaagattt aaatagatga gtacataatg
      121 tataaattgg gaatatacag tatgacttac ggtaatatat cagtatattg atatattatt
      181 tgcacatggg agtatgctat tttttatcta tacatcttaa aaaaatttac agttttataa
      241 ttcaatatca ttaaacaaac aacatattca gcttattttt cattctctta cgttacaagt
      301 tgtaaatacc atcacgttaa acaacaatag ccaacgctaa taaaatatat tgcgttaaag
      361 tggagtgatt aatatgtcaa acgtactaac agaaatagat agtcaatatc catatatgac
      421 taaaaatgaa aaaaagatag ctaaatttat actaaattca cctcaaaagg tgattaaaat
      481 gaggtctcaa gacttagcaa gcttattaga tattagtaca tcatcagtca ttcgattcag
      541 caagaaaatt actgatggcg gttttcacga cttgaaaata aatatatcta aatatgttcc
      601 taaagcatca tcaatttaca atgttgaatt aatgaataac gaaagcacag aatctttaag
      661 aactaaactc catacacgta ctacacgcgc acttaatcat gcaaataacg aattaaatga
      721 taaaactatt gatcaaatat gtcattgttt aaaacgttct gagacgattt ttatatatgg
      781 ttttggcgca tcttttgtag ttgctaccga cttataccaa aagttatcaa gaataggtct
      841 taatattcaa cttgttcaag aaacacatat ttttgcaaca ttattagcaa ctcacaattc
      901 gaacgatagt gttattctta ttactaacaa tggtacacaa agtgaaatgc aatcaatggt
      961 taaagttatt gatgactacc atatacctat aatcacaatt actagtacaa gggataatcc
     1021 cgtagcacag gcatcaaata ttgttttaac atatgggaaa actgatgaaa atgagatgca
     1081 tatgggagca acaacttcat tatttgctca aatgttcact attgatattt tatattatcg
     1141 ttatgttgca ttaaattatc acgcatcatt agatttcata acacaatcta aaatggcttt
     1201 agataactat cgtaaacatt tatcaaatat taactttaaa cattaacagt cttatataaa
     1261 ttctttaatc atgtcatata ataatttatg catcattttc aaagcttaca tgtcttttat
     1321 cttgtataac atacgcatat gaccaatgat ttgataaggc cattctattt cttatattaa
     1381 aaacaaacgc agttgattaa cggattcatt ccatatatca attgcgtttg ttattttaat
     1441 aatgctattt ttttaagccc acagtaaaaa ctaacgatag tattgccact tataagtaaa
     1501 ttcatactac atctttgttt taagctatat ttataaaaaa tgcgcctact cataacattt
     1561 ctttggctat ttaactttaa taagctaggg ccaagtttgt cataaaataa gcgcacatta
     1621 ttatcatact attttgcttt tacattattt ttattatatc taataaaata ataaatcgtt
     1681 acaatcgtta agaagatgac accaataagc actgatattc tagtttcaac attaaatacc
     1741 ataccgacca atactaatat taagaataat attgttaagt agttacttac tgcgccacca
     1801 ggcattttga atgggtgacc ttccacttct tctgggtgta attttctaaa acgtaagtga
     1861 ctgaataaaa tcataaacca aggaatcata cctggtaaaa tagacgcact atatacgtat
     1921 acgaaaatac tcttcgctcc atcgatatat aaaggtaaaa ttacatttaa taaagcacca
     1981 attaaaatac caagagatac tgcaacaaca gtgtacaatg gcacaccatt tctcatgatt
     2041 ttagtaaata ttttcggcat ttcaccttta tgagcaagtg tataaatcat acgactcgca
     2101 ctgaaaatac ctgagttaca tcctgacatt gctgcagtta atacaacaaa gttaattaat
     2161 ccagctgcaa atgtaatacc gattttagca aatgttgcaa caaacggact tccaatgtct
     2221 cctaattgat tccaaggata cacagaaaca ataacaaaaa tagcacctaa atagaaaatt
     2281 aaaattctcc agattacacc attcactgct ttaacgatat ttttctgagg atctttagtt
     2341 tcacctgcag taatacctat cagttccaca ccttggtatg aaccaattac aattgataat
     2401 gcaaagaaga agccaacaat accatttggc ataaatccgc catttgtcca taaattagaa
     2461 atacctaccg catggccacc atttccaaaa ccaaagaaaa taagaccaaa accagcaata
     2521 atcattaaaa taattgttac aactttaatt aaagcaaacc aaaattcaaa ttctccaaac
     2581 gcttttaccg agaataaatt cgctgccatt aataataaaa tagcaataac accaggaatc
     2641 caagttggca attccgggaa ccaaaacttc atatattctc ctactgcgat gacctcactc
     2701 ataccaacaa caatccattg gaatatatta ctccatgctg tcatataacc tgctgcagga
     2761 tgtatataat cacttgcaaa tgttgcaaat gatcctgttg tagggtttaa ataaatcatt
     2821 tcccccattg ctctcatgat taaaaataaa aagatacccg caattaaata tgcaaggata
     2881 actgatgggc ctgtccattt aattgtactt gtggcaccca tgaaaagccc cacgccaatc
     2941 gtaccaccaa gtgcaatcat ttgaatttga cgggcactca atcccctttg tagttcattg
     3001 ttctccataa cttcttaccc catctctgat tgatactaaa taaatccata aaaaaactta
     3061 ttgccgtaat tatactataa attatcgatt ttggtacatt aatatttgca taccatgcaa
     3121 tcgtttacag aactactata gcaggctttt gcaactttgc aaacaattat tttgtgattt
     3181 ttctaaaaaa ttaaaaaagt tgtccatgaa ccgttgcgtc acatacaact tcagctattc
     3241 tcggtaatta tgatataatt ttaataatat taaaaaggag tatatcaatg ttaaaacaaa
     3301 tactgccacg tgctacaaaa atatcggtaa tctttgctgt agcatttttc atcataaatt
     3361 atatcggtat ggaaaaacct gacaccctat atttagttgg aagaactatt attgcaacac
     3421 ttgctttcat actcatttgt cttacagtat tttctattat taattctcca gagcgcaaga
     3481 ttaaactcgg tacgacttta ccgattgcat taattattgg tatcattttc ggcgccatat
     3541 ttttaacggt acaaatcggt gtcatcactg ggttaatcat cggtgtgatt gctacgttta
     3601 tttgggaatt gattgaaaaa aataaaggag gtcgttcatc atgacaacag cattaattgg
     3661 tgtaattgta ttaatcttac taattattag ctttgtgcca aactaccgtg ctatgaaatc
     3721 agcaaaagaa caaggaagta atgcaacaag agctacaatt atggtaggca tagatattat
     3781 cttaattgta cttatcatcg tgacgttatt attaaaatat atgtaactat ttaaaccacc
     3841 ctatctcata cataaacatg aacgttctac catttcacat acatatggtc gtccatttaa
     3901 tgaggtaagg tggttttctc gttaaagttt accttgtaat agtaaaaaat agccaattat
     3961 tgcgagtatt aagtaaatca ttgaaccaac aacaaaagat cttacttgca cgcgaaatgg
     4021 tgtctgtttg ttaaatatag gaccaatcac caaatttcca acgcctaaaa ttactggacc
     4081 agctagaaaa aacaatataa tgtaacccat catgatacgc ctcctatttc gtaaagaaac
     4141 gcgctacaat ttgatcgtaa tcaatatctt ttgctacata atctacatta gaaaagtcct
     4201 gtgcgcttgt cattttattc gtattaacaa taacatctaa tccagctgca atcgcggctg
     4261 ttgcaccatt tacagaatct tcaatagcca aacattgtgt aggcatataa tttaattgtt
     4321 gtactgcaga taaatataat tcagggtcag gtttaacttg ttcaacatct tctctaccaa
     4381 caactacctc tatatactcg tctagaccta atgctttaaa ggtaggcatt atttccgctt
     4441 tcacactact tgtggcaatc gccataggaa tgtgacgttg ctttaaaaat gccatcaatg
     4501 atttaatcgt atcaatcatc ggtaatgttg tactagtaag atgatgttct tcataaagtt
     4561 tatctttatt ttccgaacca atcgctttaa ttaaatggtc atgcaaatct gtagctgctc
     4621 ctccaataga agaacgataa aaatcaatgc ttataggatc ggcattatgc atctctaaat
     4681 gtttattaat aacattaaat aaatgttgtt ccgtatctat tattgttcca tcgaaatcaa
     4741 atataactgc tctatacata tatttactcc tctatttttt ctctttatca aagcttaaca
     4801 cagtccgatg accacttcat tctattagtg caattataac ttttttgacc aatactttgc
     4861 taaaataggc cctgttatat tatgaatgaa actaaacact gcgcctggta ctgctgcaat
     4921 tggattaaaa tgcaatgctg ctaatgacac agctaaacca gagttctgca ttccaacttc
     4981 aatactgact gctttttgat cttgtcgatc taattttaaa agacgagcta accaatatcc
     5041 aatcgtatac cctaatacgt tatgtaacat aactactaaa aatattaaca atcctgtagt
     5101 taagatttgg tgcttactgc cacctacaac tattgctaaa attaatgaaa tagcaacaac
     5161 tgatataatc ggcaaagctg tagaagcttt ttcagcaatt ttacgattaa taatttacaa
     5221 aacaatacct aaagcaattg gaattaatac aacttgaaca actgaccaca acatactcag
     5281 gaaagatact tccaaccatt catttgcaaa tagatatatt aacgcaggtg taacgaatgg
     5341 cgctagcaac gtagagaccg tcgtaataga aacagaaagc gctacgttag ctttggctaa
     5401 ataactcatt acattacttg atgtcccacc cggacaacat ccaactaata ttacgccaac
     5461 agcaatatca ggtggtaaat gaaaagactt tgcaattata aatgctaatg tgggcataat
     5521 actgaattgt aggcagacac caataattac tgctctaggt gctttaaaga ccattttgaa
     5581 atcattaggt gtaattgtta atcccatacc taacataaca ataccaagta aataaggtac
     5641 ccacttacct aatgtcgcaa catgttgtgg gaaaataaat ccaataacag ctgctactaa
     5701 catccatacc aaaaaactct tcgtggcaaa tatactcaat ttttgaaaca ttgttaacac
     5761 atcctcgtcg ataatattga aaaatattac cataagacca gtaaggttac aattactttt
     5821 ctgaaaattg agattattta gccattctct ttttattgca aatattttat cactgataaa
     5881 caattatttt aaacttcatt caaaagtctt agtgaataaa gaaaaaaagc gtggtaaaat
     5941 caacttgaac aaaattaagt cacctttata aatattaaac gtgtatttaa tgccatgtat
     6001 tcctcgggag gtcctcacat gttaattgat aaattcgaaa catacatcat caacatcgcc
     6061 ggtttaaatg accgtactac tagaaaaaaa ttaagtaagt tatgcaaaag tgtacaattt
     6121 tgtgatgcgt tgcaattctc aataaacaaa cagtttaatc agtatgtatt agaaattagc
     6181 ttaccaaagc aacaacttcc atacttcatt tcatttttaa gttttcatca gtatagtatt
     6241 ttccaagtac tttcaccaaa gaaaataaat gaattacttg attctgataa tttatatcaa
     6301 tctgcaaagc gtttcgatat caatattgat ggcttgcaag atgctttcat taaagacaaa
     6361 gtcatcgata ttatgaatat gttccaaaat cacactgata tcacttatac gttgaataag
     6421 tctcatgcgc atattatatg tacgccagaa atattcgcta aattgttaca tacgattgca
     6481 actagaaata tcgacattct atctgccaat tatagatcgt catctatgag caaagctcgt
     6541 atatcataat aaaagacaca tctatacgat gatcatttac aatgattgcg tatagtgatg
     6601 tgtctttttt tgtattattt atcttcggac gattgatcct caaccatttg ttcaaatgct
     6661 tcacgaactt gtggtactgt catacctact acaacttgga tactcttacc actctttact
     6721 aatccgtgtg ccatttgttg atgcgtgaaa tattccgtat cctcaacttt actttcatca
     6781 taaacagtta agcgtaatct tgttgtacag ttagtaacat ctttgatatt ttctttgccg
     6841 cctaaaccgt ctaggtaata cgcagctttg tcttcgtatt cattaccagt tgaagcacgt
     6901 ttagggtcaa cgttatcacc ttttttattt ttatagtctt gtttagagaa taacttaacc
     6961 tcttcttctg ttttcttacg tccaggtaat ggaatatcaa atttcaaaat taagaatctg
     7021 aataagaaga agtaaatcgc tacaaatatt aagcctataa caacttgtgc tacataagtg
     7081 ccccaatgtt ctttcccaag tggtaaccag tttgttgaaa taaagtctaa caaaccacca
     7141 cccatgttac ctacaacacc aaatgcgtac attaaagtat ccattgatgc tgctaaaact
     7201 gcatgtaaaa caaataagta tggcgcgatg aataagaatg taaattctaa tggctcagta
     7261 atacctacaa cgactgctgt taatgtggca ggaataagta gtgccgccac ctttttacga
     7321 ttttctttag gtgtcgtagc atacattgct aaggcaatac ctaaacatcc aaatactttc
     7381 ccatttcctt gaagcataaa gccataaggg aattgctctt ttaatggctt actactctta
     7441 gcaaactcat ttaaatgttg taaccattca gctttcaagc cgtgattgac tactactggt
     7501 ccaacttcaa ttggcgcata aataaagtga tgtaatcctg ttggtattaa tacacgttct
     7561 aagaagtgat acaaccatac accgatatag cctgaagcaa tgataaagtg ttgcattgag
     7621 ccaataccgt gctgaacagt tggccaaata acacaagtaa ttgctgctaa tggtaacatc
     7681 acaaaaaatg agattgtaac tacaaatgtt aaaccttgga atactccaac catttctggt
     7741 aatcgtttac tatagtatct attgtgaatc caagtaataa tagctgaaat aataatacca
     7801 cctagaatat tcgtatccaa tgtggcaata cctgcaattg attttaaacc aggtacattt
     7861 tcaacgcctt tttctaaatt agcgccaaac gtatgtggcc attgagttaa aatggcattt
     7921 ataaatgtat taaacattaa gtaacccatc aatgctgcaa gtgctgcatg acctggtgct
     7981 tttttagcta aagaaagtgg taaacctaca acaaatacaa cttccatatg cgtaaaaata
     8041 acccagccac cagattctat tactgaccaa aatttaaacc aaaacgtatg ttgatctgct
     8101 aaactcccca tgattgtagg atttttaaat aatgtcgcaa agccaagcac tatcccaaag
     8161 aaagcgaaca ttaatactgg tacaatcatt gcactaccga aacgctttat cgcattcatc
     8221 ttctattccc tccatatcat ctttcctaac aatacatcta attagattca tttataaata
     8281 gatgccttac tatttaaata taatatatag taaacgctta cacacctaca acgacattga
     8341 cgtattttga aagtattttg tataatcaga ttatcttttc atatagtgaa aattttttca
     8401 caagcatgtc gagaatatgc tcgattttaa aatatgtaag tactatagcc atgtataacc
     8461 ttatatacaa tcaaaaaatt aatttctaaa atttcacctt ttaatgtaaa aaccatatat
     8521 cacaaattta ttttccaagg aggttgaata tgtttttaga cgaacacatt aatagaaact
     8581 ttgacaaact aaatgataat gatttacata tcgcccactt catcaacaca cacgttgatg
     8641 aatgtaaaaa tatgaagata caagatttag cgcaattcac acatgcctcg aatgcaacca
     8701 ttcatcgatt cacacgtaaa ttaggttttg atggttatag tgattttaaa tcgtacctta
     8761 aatttgaaag taataagaca caccaattac catctaactc tatcgatagt ttcaagcaag
     8821 aaatcgatag tacattcagt tatttagaac gcgttgatta ccagcttctt actgaaaaaa
     8881 tgcatacagc ttcaacaata tatttgtatg gtacagggcg tgcacagtta aacgttgctt
     8941 cagaggcaca gcgtatatta ttaacgttac ataatcatgt cattgtactt catgatgaac
     9001 atgaacttaa aatgattttg aacaagagtg aacctaatga tttattcttt attatttcat
     9061 tatcaggtga aacacatgaa ttattagaag taacaaactt gcttcaactt agacaaaaat
     9121 actttatttc tgttactaca atgaaagata atacccttgc tcaaaaagca aattacaacg
     9181 tctatgtatc cagtaacaca ttctacttaa ctgatggtac agactattct agcttcatca
     9241 gttatcatat attctttgaa acacttgtaa gaaaatataa tgagtattta ctacataatg
     9301 atatttcaaa ttagaaaaaa ggaagttatg atttaatggc cttgagccta tcataacttc
     9361 cttttatcat tttattgttg tgttgatgtt tcgataacgt ggtacatttt atcaaacatc
     9421 aattcgaaac catgcaccat ggaatcatga tattcttttt tcttttgctt gtattctaaa
     9481 ttagtaaatc gtctttcctt ttcaactaat gaacgataat aaaatagcat ttgggtgcca
     9541 cctgtttcac gttcaaaaaa ttctacctca atgacatctt gtgtttcact tagtccaggc
     9601 ataccgatag tcatcttaac gtattcatcc ataactaaag attcataaat accttcaatc
     9661 acatttactt tgccattacg ttgttgatct acaatacgat aattaccgcc ttctttaaca
     9721 tccgcttcaa tctctttatt cgttctggct gatgtcataa accattgttt taacaaatct
     9781 ttctttgtcc aagcttcgta tactaactct ggagaaaatt tataaagctt ttcaatttca
     9841 acttcgacat gttcattctc tacattaaat tttgccactg ttgtccaccc actttcgctc
     9901 ttacttttat tttaacgtat ttttgctcag ttccaaacat agatgatcat catttttaaa
     9961 agattagcgt tatacggtga gtacaacatg atctgttaat ataacaagcc accttacttg
    10021 gctacatcga tatattgtta agcattacta tttcatttct tgactagtgt tcttttttag
    10081 ctttggaaaa ttaaataaaa tcgcaacaag tccgcataca cctaataata taggataaat
    10141 gctatatggg aataacatta acggtgaaat accagctaca ccagccgctg aaatgacttg
    10201 cgggctatat ggtaataaac cttggaagca gcctccaaat atatcaagaa tacttgctga
    10261 tttccttgaa tctacatcat attcatctgc aatattttta gctaaaggac ctgacataat
    10321 aatagagatg gtgttgtttg ccgtggcaat atctgcgaca cttaccaaac tcgcaattcc
    10381 taattctgcg ccacgctttg atttcacttt agagcgaaca aattgcaaca accattcaat
    10441 accaccattg tgttgaataa taccgactaa accaccaatt agcaatgcaa tcatagcaat
    10501 atcttccatg cttataatac ctttggacac tgcatctagt agccccatcc aactgaatga
    10561 accatctatg agaccaatga ttccggctaa taatgttccg ccaatcaata cgataatgac
    10621 atttacacct aataatgcta ataccaatac taaaatatac ggtacaactt taattaaatt
    10681 ataatcatag tttttagcat gatttaaaga aatgccattc gttaagaaat aaagaataat
    10741 aatcgttaaa atagcacctg gcaatacaat tttaaagttt actctgaatt tatctttcat
    10801 tttcgtatgt tgtgttctaa ccgcagcaat tgttgtatct gaaatcattg atagattatc
    10861 gccgaacatt gcacctccaa caactgtagc cattgctagc gcagttggta catctgtcgc
    10921 ttgtgcaaat ccgtaaccaa caggtgctat tgcagcaaca gtccctactg acgttcccat
    10981 agatatagaa acaaacatac aaatcacaaa caatcctaca ataattaaat tttctgggat
    11041 taatgatagt cctaaattaa ctgtcgactt tacgccaccc attttttcag ctgtatttga
    11101 aaatgcacct gctaaaataa aaatcaacat cattaaaata atgtttgaat ggcctgcacc
    11161 tttcgtgaaa acctcaactt ttttagcaaa tgattctttt cgattcatta ataacgccac
    11221 aattaccgtt atcgtaattg caacatttaa tggcattgaa gtaaaatcac ccgtgataat
    11281 acctacgcct aaaaacaacg ccacaaataa taacaagggg aataatgccc aagcattgct
    11341 ctttttatgt acttccatcc tttttacctg ctttccaatt aaaaatacct ctttctcaca
    11401 aacgatgaag aaagaggttt tcatgtactt tacctgctta tcttcaaacc attacggtta
    11461 ctggaattgg cacattcgag atgttgccga ggcttcatag ggccagtccc tccacctctc
    11521 tagataagtg atgcttattt acgtttacgt tacaagataa tccttagtac gtcaatcata
    11581 aattaattag gagtcgtata atatttttca taaacaatca ttgctactgt aataataatc
    11641 aaaacaataa tgctaatcgc aagtataagc caccatttaa gcattaatgc aataaaaatg
    11701 aacacgatag acacacttac taatattaat gatatgactt taaattgctg tgcactttgt
    11761 tcagaaataa ctttcaactg tttgtttgat ggacgcgtat ttcttatact gattcccagt
    11821 atattttcta atatttgaac caatacgata cttattgcaa atataataat tggtaaaaca
    11881 tcatagctcc ctatagttaa tgtataaatt acaaatccaa tgtaaagtaa ccctgagagc
    11941 acacccaaaa agtaaacaac atgtttgtca aacttaatga tatgtttttt aacgttttga
    12001 tgtgtaagcc atacattcga aacaatcgca accacaacaa acagtgtgaa taatatataa
    12061 actggcaact tttgttcagg aaaaacagtc gctattccta aagctaatgc taaaatcaaa
    12121 aataaaatag ctctagatac tattaatgcc ataataacaa cccctttgtt taatatcgag
    12181 tttgcaaatt tacgtttatc agcatttcta tgatcagtac ttctacggtc agcgtttcta
    12241 tgtaatttac atcatcttaa catataaata cttcgctatt taattgaaaa catatcctat
    12301 tattctttgt ccgttctgac gtctaatatc taaccttagg cattacactt ggtaataaat
    12361 ttaactttct tccactaacc gccactaaac ccaatcccgc aatagttttt aactttttcg
    12421 ttgttgtcct gacatcctca ttaagaaagt ttattctgct taaaacttat aatccacacc
    12481 ctgagcaaac gctccttatg acaaagtatt aaaataagcc gataaagata cacaccttta
    12541 ccgactattt aaaatacact tcaccaattc attttaattt acttgattga agtagctaaa
    12601 ttaatattat gttgttcaat taaaagcttc atacaaacct aatctatttg cactccaccg
    12661 ctaacaccga acacttgtcc agttgtataa cttgattctt ctgacgctaa tagcacataa
    12721 gttccacata actcaacagg ttgacctgca cgacctaaag gtgttttttg accaaatgtt
    12781 gggattttac tttgaggttg tccaccagaa atttgtaatg gtgaccagaa tggaccaggc
    12841 gctacacagt tcactctaat tccttttggt cctaattctt ctgaaaaact tttagttaat
    12901 gaaataattg ctgcttttga agcggcataa tcatgaagaa taggactagg attataacct
    12961 tgtacagacg atgtcgtcgt aattgacgca cccggtttta aatattccaa agctttttga
    13021 actgtccaaa atagcggata gacattcgtt tcaaatgttt cagtaaatgc ctcagttgta
    13081 aatccatgaa tatcatcatg atactgttga tgtccagcaa ctaaagtaac attatctaag
    13141 ccacctaatt gttgatatgc ttgttcaaca aggtcatagt tgaactgctc atctcttata
    13201 tcaccaggaa ttaacactgc cttttgacca ctttcttcaa tcacttggcg tacttcttgt
    13261 gcatcttgtt cttcactcgg aagatagtta atcgctacat ctgcaccttc tttagcatac
    13321 gcaattgctg ctgcacgccc tattgctgag tcaccacctg taactaacat tttatagcct
    13381 tgtaagcgtt gattaccttg gtaagacgtt tcgccacaat caggtgctgg cgtcatttca
    13441 gattgtaaac ccggtacctc ttgctcttgt ttttcataat ccgtggtttt aaattttgtt
    13501 ctaggatctt gagctgccat ttttaaacat ctccttattc gcttaatggt tattatttac
    13561 ccaatcttcc taggaactta atcatgatta cactaaaaat tactttcttc tttataaaaa
    13621 caagctcgaa ttactcatgc aatagtctct ttacaaattc aacaaaatac tcaggtactt
    13681 tttctagaat cctttcatcc ggtttatatt gagggtgatg taaatcatat tcactatgag
    13741 aaccaattaa cgcaaataca cttggaaaat gttgactata acctgaaaaa tcttctccaa
    13801 tcgtaagcgg ctgttccatc atttccacct tatatccaac atgttaggct actgctattg
    13861 ctttatgcgt caatgcctca tcattcatca cagcgccagg taaatgtgta taatttaaat
    13921 taattttcat attatatgct tgagctaacc cgtccgcaat atcttgtaat cgtgtttcta
    13981 caagctttcg caccacagga tcaaaactac gcactgtgcc ttgtacatac gcatgatcag
    14041 caatgacatt ccaagtatta ccacatgata tttgtccaat tgttactacc gcttcatcaa
    14101 acgcagatag atttctacta actatggatt gaatactatt aatcaattgc gccaacacaa
    14161 taactggatc gttgcattgt tctggctttg cagcatgacc acccacgcct ttaatatgaa
    14221 actcaaaacg atctactgct gatgtaattg cccctgtttt gattgcaaat gtacctaccg
    14281 gacgcgatgg gtcattatga aaacccaata ctgcttgtac atcttgtaat gcatgtgttt
    14341 caataatttt aaaagcgcca tgtcctagtt cttctgctgg ttgaaaaatg aatttaacac
    14401 gcccagtaag agtgccctca atttctttta actttacagc tgtagccaaa atactagcca
    14461 tgtgaatatc atgaccacac gcatgcatca caccttcatt ttcagattta aaatcctgtt
    14521 caacaagctc ttgaattggt aaagcatcaa tgtcagcccg aacagctata caactcagac
    14581 cttgccccac ttcagcaaca agtccagtcg caagtggtaa gtctaatatt ctaatattat
    14641 attctgttaa aatatcttta attttttgtg tagtcttaaa ttctttatcg gatagttctg
    14701 gaaattgatg aaaatacctt ctccaggcaa cagcttgatc ttttaatccc atcggtcatt
    14761 ccccttcctt aagtcaatga tatgttgtct accctacgat gatcgtcttt aactattaaa
    14821 cgatgatttc acaacaatgt actcttgtta attactttcg ctaatgatag acagttgttt
    14881 aataatatcg taacattatt gtcaaactat tctaactttt ataattaaga acctatacaa
    14941 aaacgtgttc tcagataaac ttgttttaca aaccacaaaa agctctaaac attagtgtaa
    15001 accaatgctt agagctttta attactttat actttaaaag ataatgtgtt atctacgatg
    15061 accttaccgt ctttaataac tttttctgcg tgattgatac caaaatgata aggaatatat
    15121 tcatgatttg gtgcatccca aattactaaa ttagccttat cacctgtgtt aattgtaccc
    15181 gcgttaatgt ctattgcttt agcagcattg accgtaacag cattccaaac ttcattaggc
    15241 gatagcttta atttcaacgc tgcaatcgcc ataacaagct gtatgttgtt tgtgacacta
    15301 cttcccggat tataatcagt tgctaatgca atcgcaccgt tattgtcaag catgcctctt
    15361 gcatctgcat aatcttcttt acctaaatag agcgtcgttg caggtaagag gacagctaca
    15421 gtatcactat ttcgcaactt ttcttttcct ttgtcactag acgctactaa gtggtcagct
    15481 gatattgctt gttcgtcaat tgctaattcc agtccgccta atggatcaat ttcatccgca
    15541 tgtattttca ctttaaaacc tgcttctttg gctttttgca tataatgttg cgattgctct
    15601 attgtaaata cacctgtttc acagaatata tccgcaaagt ctgcataatg ttttacttcc
    15661 ggaagtaacg caatcacttc ttctaaaaat gcctcatttg aacttgcctc tttaggtaca
    15721 gcatgaggcc ctaggaaagt atgtttcata tctaaatcat atttctcagc taaacgatta
    15781 gacactttca attgcttcag ttcattttct ctatctaatc cataaccact cttactttca
    15841 actgcaagca cgccgtgttt aatcatagta agcaaatcat gctctgcttt tttaaacaag
    15901 tcatcttcgg atgtttctct agtagcatta acggtagata atatgccacc acccatttct
    15961 aatatttcaa ggtaagactt accttgacgt tttaatgaca tctcatgttc tcgagatcca
    16021 ccaaatgtta aatgggtatg agcatctact aatgctgggg acactacctt cccactagca
    16081 tcaattgtct cagtcgcatc gtagtcatct gtatgtgttc cagcatatac aattttgcca
    16141 tctttaatga caactgtacc atttttcaca acatttaatt catctaattc cttacccttc
    16201 aaaggtttat ctgttgatct cggtaaaatt aattctgcta tatgattaat tattaaatca
    16261 ttcattactt atcacctgct ttatcaatca ttggaatatg aatacctttt tctttagctg
    16321 tttgaatagc aatgtcatag ccagcatcaa catgtcgtgc aacacccata cctggatcag
    16381 tcgtcaatac gcgttccaat cttctttcag cacgctctga tccatctgct acgacaacca
    16441 tacccgcatg aagtgaatat cccatgccaa caccgccacc atgatggaat gaaatccatg
    16501 aaccacctgc agccgtgtta atgagtgcat tcaatacagc ccaatcacca accgcgtcac
    16561 taccatcttt catactttct gtttcacggt taggactcgc aactgaacca gcatctaaat
    16621 ggtctcgtcc aataacaatt ggtgctgaaa tttcaccgtc acgtacaaga cgatttaaag
    16681 ctaagcccat ttttgctctt tctccatagc ctaaccaagc aatacgtgat ggtagtcctt
    16741 gatatgaaat tttttcttca gctaaatcaa gccatcttaa taacttttca ttttctggga
    16801 aaagtttacg catttcttca tccgcacgct cgatatcttt tggatcacca ctcaatgcag
    16861 caaagcggaa tggcccctta ccttcacaga ataatggtct aatgtaagct ggtacaaagc
    16921 ctgggaagtc aaaagcattt ctcactccgt tattgaaggc tacttgacga atattgttac
    16981 cataatcaaa tgctacagct ccacgtttat ggaattcaag cattaattca acatgctttg
    17041 ccattgaagc ttgtgacagt tcaacatatt ttttcggatc tttttcacgc aatactttcg
    17101 cttcttctac ggagtatcct tgtggcacat atccatttag cggatcatgt gcacttgttt
    17161 ggtcagtaat aatgtcaatt ttaaatcctt tttctagaat cgcttgatgg aggtctacag
    17221 catttccaac taacccgatt gacaatcctt ctccacgttc tttcgcttct tctgctaatt
    17281 ttaatgcttc atctaaatta gctgttttaa tatcacaata tttcgtagca attcgcttat
    17341 caacacgtgt ttcatcaaca tccacacaaa ttgctacccc atgattcata gtaattgcta
    17401 acggttgcgc accacccata ccacctaaac ctgctgtcag tgtaacagtg cctgctaaat
    17461 ctccattaaa gtgttgatta cctagctcag caaatgtctc ataagtacct tgcacaatac
    17521 cttgagaacc aatatatatc caactaccgg ctgtcatctg tccatacatg attaaacctt
    17581 ttttatctaa ttcattaaaa tgatcccagt ttgcccattc aggcactaat actgaatttg
    17641 aaattaatac acgtggcgct tcttcatgtg ttttaaatac agcaactggc tttcctgatt
    17701 gtactaacat tgtctcatct gattctaatt cgcgtaacgt tttctctatt gcttcaaaag
    17761 cttcccaatt acgagctgct tttccaatac caccataaac aactaaatct tctggtcttt
    17821 cagcaacttc tgtgtctaaa ttgttgtata acattctaag tactgcttct tgttcccaac
    17881 ctttacactc aatactcaaa ccttttttgc ttgaattttt ctcataaaat tcgctcctgt
    17941 tctttttaga agttaattcc actaaattta aaacgcttac attattatct tcaatattca
    18001 ttatagtatg ttaaaatata gccaacaaat ataaataaac taattatcca tagcttgaat
    18061 ctataaataa aaggagcaaa aaacatgaaa attattcagt tagaatactt cttggctatc
    18121 gtgaaatata atagttttac taaagctgca caatttttac atattagcca gccatcttta
    18181 actgctacga ttaaaaaaat ggaagcagat ttaggttatg acttatttac acgttcaaca
    18241 aaagacatca agattaccga aaaaggaata cagttttatc gttatgcgag cgaattagtt
    18301 caacaatatc gatccacgat ggaaaaaatg tatgatttaa gcgttacatc agaaccaagg
    18361 ataaaaattg ggactcttga atctacgaat caatggattg cgaatttaat tcgaaaacac
    18421 cattccgact accctgaaca gcaatatcgt ttatatgaaa tacatgacaa acatcaatct
    18481 atagagcaat tactgaattt taatattcat ttagctataa caaatgaaaa aataacccac
    18541 gaagatataa gatccattcc tttatatgag gaatcttaca ttttattagc acctaaagaa
    18601 acatttaaaa atcaaaattg ggtagatgtt gaaaatttgc cactcatatt gccaaacaaa
    18661 aattctcaag tgcgcaaaca cttagatgac tattttaata gaagaaatat tcgtccaaat
    18721 gtcgttgtag aaacagatcg attcgaatca gcagttggat ttgttcatct tggcttaggt
    18781 tacgctatca ttccgagatt ttattaccaa tcatttcaca cgtccaattt agaatataaa
    18841 aaaattcgtc caaacttatg ccgaaaaatt tatatcaatt atcataaaaa gcgtaaacac
    18901 tccgaacaag tacatacatt cgtacaacaa tgccaagatt atttatatgg acttttagag
    18961 gctctttaac ttaagttatt agagcctctt atgcagttgc tcagtcaact gtataccttt
    19021 tgcctttaac ttaagttatt agagcctctt atgcagctgc tcagtcaact gtataccttt
    19081 tgcctttaac ttaagttatt agagcctctt atgcagttgc tcagtcaact gtataaaaat
    19141 taatgacgtc atttcaaaaa tcgatacaaa aataatttat tataaaaatt ctaagaaaga
    19201 agtgaacccg atgttaaaat ctattaatca tatatgcttt tcagtcagaa atttaaacga
    19261 ttcaatacat ttttatagag atattttact tggaaaattg ctattgactg gtaaaaaaac
    19321 tgcttatttt aagcttgcag gcctatggat tgctttaaat gaagaaaaag atataccacg
    19381 taatgaaatc cacttttcat atacacatat agctttcact atagatgaca gcgaatttaa
    19441 atattggcat cagaggttaa aagataataa cgtgaatatc ttagaaggaa gagttagaga
    19501 tattagagat agacaatcaa tttactttac tgaccctgat ggtcataagc tagaattaca
    19561 tactggcaca cttgagaaca gattaaatta ttataaagag gctaaaccac atatgacatt
    19621 ttacaaataa ggcgtcatta taaaaaggcc tcttgaactc cgttaaaatt ttaattaatt
    19681 attatataat aagagaactt ttcaaacaat acagttgtta tttttactat ctcaacaaac
    19741 ataaataagc agtaagatga ctacaactta agagtcttct tactgcaatt atctttcaaa
    19801 tatatcaacg ttaatataac ttctattacg aaatactcac attctgccct gcaatgcaaa
    19861 tttcgtcaca tataaatatt tttaattatt ttaaaaaatg atgcactaaa ttagcaacga
    19921 gcttcgcagt tctattgtca gcgtcatatg ttggattcat ctcagcaata ctaactgaag
    19981 acaccttatc acttggaata atacgttttg ccaattcaag aacagtatgt ggatacaaac
    20041 ctaacactgc cggcgcactt accccaggcg caaatgcact atcaatgaca tccatacaaa
    20101 tcgtaaacat aatgacatca tgttcatgta taaaacgctc aatcatatct ttgattgttg
    20161 gtgatacgtg actcaataat tcatctgcaa agacataatc aatctttttc tctttagcat
    20221 aatcaaataa actttgcgta ttcccacctt gagcaatacc aagcactaaa taacctgtgt
    20281 tttcatcttc ttctaaaatt tgtctaaagc tcgttccaga tgtagattgt tgttcagcac
    20341 gtgtatcaaa atgcgcatca atatttatca caccaataga ttgtgttgga tagactttac
    20401 gcgttgctaa atattgagca tacgcaatat catgtccacc acctaataaa aacgtttgtc
    20461 tatgattaac aattgacttc gctgcaagtg ttgcaaattc tttttgagta tcaattaatt
    20521 cctcatgatc atgataaaca tttccgtaat cgactaaagt ttcacattga ttcaaatccg
    20581 gcaaacctgc aaatgcttgt ttaatcgcat ctggtccttc ttttgcacca atgcgcccct
    20641 tgtttaaagc aacacctttg tcaacagcat agcctaatat accaacccct gatggtgtac
    20701 tacttttttc caatttcgac aaatcttcaa atgtcactgt ttgaaaatgc ctgaattttt
    20761 tcgggtctgt ttcactatct aaccttccag tccataaatt tggttcacct tgcttgtaca
    20821 cagcatttcc ccctcttatt tatgtggctt tttaacaatt aaagtataac gtactacaaa
    20881 ttttgaattc aattcataat taaaaagtac ttatctatat accttttacc cctattttct
    20941 attcactaac gaacacgaaa gcgatttatg ttaataaaat acgtcaagac tattacattt
    21001 tcattaatat tagcacagac aatttaactc acggcttgta atatgtataa ttgttacaaa
    21061 atgatatttt gcttgttacc taatggaggt tacatataat gaaaaacaat aaaatttctg
    21121 gttttcaatg ggcaatgacg atcttcgtct ttttcgttat tacaatggcg ttatccatta
    21181 tgcttagaga tttccagtct gtcattggtg tcaaacactt tatatttgaa gttacagatc
    21241 tagcaccatt aattgctgca atcatttgta tactcgtttt caaatataaa aagatccaac
    21301 ttgcaggttt aaaattctca atcagcctga aagtaattga acgtctactg ctagctttaa
    21361 ttttaccttt aattattcta attattggta tgtacagctt taatacattt gcagatagct
    21421 ttattttatt acaatcaaca ggcttatcag tacctattac acatattctg attggacata
    21481 ttctaatggc gttcgtagta gaattcggat tccgttcata cttacaaaat attgttgaaa
    21541 cgaaaatgaa tacgttcttt gcaagcatag ttgttggttt aatgtactct gtattttcag
    21601 caaatacaac ttacgataca gaatttgcag cttataattt cctttacaca ttctcattct
    21661 caatgattct aggtgagtta attagagcta caaaaggacg tacgatctac atcgcaacaa
    21721 catttcatgc ttcaatgaca tttggcctta ttttcttatt tagcgaagaa atcggtgatt
    21781 tattttcaat taaggtcatt gctatttcaa cagcaattgt agcggttggc tatattggtt
    21841 taagcttaat tattcgaggt attgcttatt taacaactag acgaaacctt gaagaacttg
    21901 agcctaataa ttatttagac catgtcaatg acgatgaaga aactaatcat actgaggctg
    21961 aaaaatcttc ttcaaatatt aaagatgctg aaaaaacagg tgtagctact gcatcaacgg
    22021 tcggtattgc taaaaatgat actgaaaata ctgtggctga cgaaccaagc attcatgaag
    22081 gtactgaaaa aacagaatct caacatcaca tagataatca aactgaatct aatcatgatg
    22141 aagatcatga catcacttcg aagtcagtag aatcagcaga atcagttaaa catgcaccac
    22201 aaagtgacga tttaacaaac gattcaaatg aagatgaaac agagcaatca ttaaaagaac
    22261 ctgcgaccta taaagaagac agacgttcat cagttgtaat cgatgcagaa aaacatatcg
    22321 aaaaaactga agaacaatct tcagataaaa acaaataata acaaagcgct tgctagtacc
    22381 tcttaaaaag atgatgctag caagcgcttt tctatactat atattatttt tctaaaattt
    22441 taacgccctc ttgagtacct acaataactt gatctgccat atctaagaag tagcctgtct
    22501 caaacacacc tgtcagatga attaaatact catggaactt atatgggtca attccttttg
    22561 gtaacttaca atctaaaata taattgccat tatcagttat aaatgcgaca tcttcgttta
    22621 cacggcgttc tacttttata tcagcgtatg attcaatttt acgtaatatg tgataccagt
    22681 taaatttatc cacctctact ggtaacttaa acgtctcacc taagtattga accattttcg
    22741 tttcatcgac aaccacaaca aaacgtgatg ccatttcatc tatcactttc tctctgaaca
    22801 gcgcaccacc accaccttta attatattta aagatggatc tacttcatca gcaccatcaa
    22861 ttgctaagtc gatatgatca acatcattga tttcgcatat tttaatacct agttcttttg
    22921 ctaaaaatgc aattttatta gaagtgcata cacctgtaat attgtaacca cgttctttaa
    22981 ttagttgcgc catttgaggt aagagtaatt ccattgtact tcctgtacca attcccagcg
    23041 tcatgtcacc attgatttga cttaaaacat catttaatgt cattaacttg agtgctttga
    23101 catctttcat gaaggtagcc tcccatattt atgtaatcta ttcaattcat attttacatg
    23161 actcgtataa attaacatac ccttatttct aacatttttt taaacatatc aatcagaatt
    23221 tacaattcta aaaatttttt atagtacaat atactttaac ttaacgttaa ggagaatgat
    23281 atgatatcaa ttcacgcaat ttcgacagga aaaatccaag atttgcctta tagctcgaaa
    23341 agaccgatgc gctctgcttt agataaaact aagatttcac aaacaacatg gttatcttca
    23401 actggtttca ctggtgatga acaggcttat aaagatcatg gtgggccaca taaagcagtt
    23461 tgtgggttta gtaaacataa ctatgcactg tatcaagatg atttaccttc actacctact
    23521 catgcgatgt ttggagagaa tttaacattt gattatttag acgaatctga tgtttacttt
    23581 ggtaatcaat atcgtttagg tgaagcgtta attgaggttt ctgaaattag agaaccatgc
    23641 tggaaaattc aagcaaaata taatattcct gatttagtga agcgcatgtc tacatctggt
    23701 aaaacaggtt tctatttccg ggtattaaaa caaggctatg tatctccaaa tgatcagctt
    23761 tacttaatac aagaagcacc aatcgaacat cgtttatctg tacaacagct taatgacctt
    23821 tattataatg atagacaaaa tcaagatatg ttacgatatg cactaaacaa tccatttctg
    23881 tcaccaacaa gacgcgataa acttcaaaaa atgtataaca gaacattgaa ataattacct
    23941 ttcattataa atgttaaatg aacttttcaa aacaaaaagg aatcaactta cacatcgttt
    24001 gtataatagt cttatctata actactgatg ctattgattc cttttttatg ttatatattt
    24061 tctttatttc aaactgtata ttaatgatgt cacttcattt gatacgattc ttgataacct
    24121 attcaaaatt ccgccaaata acataaatat tatataaatg ccgatacttt taatcatttt
    24181 ctactttttc ttcgatacgg aaacttgttt tcgaattgaa cacttcacca gcttttaaaa
    24241 ttgacggtgc tttttcacca tataaattaa tatcatttgg taaaaattgt gtttctaatg
    24301 taaagccaga atgtggttta taaatattaa atggactatc ccactcatca ggctgattaa
    24361 aagtaaagaa tacaacatga ggcatatctg tatcgacctc taacataaat tcatgatttt
    24421 caacatacat tttatgttca ccaactgtaa atgggtgatc tagaccacca aaacgtgtca
    24481 tttgttggct taattgcgca tgttgtgatg taaaaatatc actcaatttt atcttgttat
    24541 cttcaaacac ttcatgtaaa tctagaatat cacctgtaac aatatttcgc tcatctaata
    24601 catacatatc taattgatta cttgaaatgc gatgattatc aacgacatta ttacctcgat
    24661 tcaaattgaa gtacacatga ttcgtaggac taaacaatgt gtcttctgat gcaactgctt
    24721 cgtattcaat cgaccattgg tgatccgcat cataaatatg tgtaatcgtc acatcgatat
    24781 cacctgggaa atgatcatca gctgatttca acaccgtttt aaatatcact ttaatttgag
    24841 caatttcatt tctaatttca taatcaaata acttattgtc caaaccatga catccaccat
    24901 gtaaatgatg ttcgccgttg tttttttcta actgatattc tttaccttgc aacttaaatt
    24961 tagcattatc aattctaccg ctatatcttc ctatagaagc accaaattta aaaggattac
    25021 tatgataaaa ttcatccgct tcaacaacat ttccaagaac aatattatta tcatgatatt
    25081 tccaagacac tactcttgct ccataattcg taaaaataat tttagtttca tcattatcaa
    25141 ttttgattaa atctacacct tgtctttggt gctcaacttc aactatcatt tttacttctc
    25201 ccttctaacc acaagtgttc aagctctgct gggcagcaac attactaaaa cacctacaat
    25261 acaaatgatt gcaccgataa catcatattt atctggcatt tgtttatcta cgaccatcgc
    25321 aaaaatcaaa ctcatgatga taaatacgcc accatatgct gcatatactc ttccgaatga
    25381 tggaaatgat tgaaatgtcg caatgacacc atataacatg agtatcacac cgcctattag
    25441 cccaacaagt gaagactgtc cttccctaag ccacagccaa atcaggtatc ccccacctat
    25501 ttcacataag ccagctaata taaatataaa aatcggatat aacatgaaat cactccatca
    25561 cacatttgct accaataatc tatcggctac atatcatttg tttacatttc ttcttacttc
    25621 acattcccat tttaaaaagt tcgttttcac attcatattg tacacttttt tagacattat
    25681 tctatagcta aatataaaaa aataagagta acacgctttc atcatcattt tatatgataa
    25741 atgtgtgtca ctctcatcaa ttttattttt taaatacacg tttcattgaa ttaaataagc
    25801 cacgttcaaa tgtaagtact gaatctttat atgttttaat tgcaatccat atcaagacag
    25861 ctaccattac aattgagatt aatgaactta agatgacttc atgtaattga agccctgaag
    25921 tttgagcgcg tacaactaat tgaaatggcg ccaaaaacgg aatataactt gtgattaaag
    25981 caagtcgccc atcaggatta tttatcgtga atatggcgat ataaaatgca atcataccaa
    26041 gtaatgtcag tggcatcaaa gattgattta aatcttctat tctagatgtt aatgatccga
    26101 gaatggctgc aagtaataca tacgccataa ttccaacaat actacttata attccgacaa
    26161 taataatttg ccaagacaat tgattcattt ccacgttaaa accttgtagc aagtctttta
    26221 agtcaaaggc aaaaatgcat atgactgcca tcaatacaat taaaataatc tgagtcacgg
    26281 caactgctat aatgcctaaa attttagcaa aaatatgttg gattggtgaa atacttgtaa
    26341 taatcatttc aatgacacgc gatgttttct cactagcaat ttccatagct atttgagatg
    26401 cataatttaa aacaataaag aacattagaa agataatgcc ataagctaaa gcatagttaa
    26461 aaatcttttg tccttctgat actttatcga cttcatcatt agaaatcacc ttattatcaa
    26521 ctttactttg tgcttgtaat ttttgtaagt cttctttgtt gatatttaat tccccggcta
    26581 ccatatttgt ttgaatagct gtaagtagtg cttgtacttt ttgtgaatct tcatgactta
    26641 ctcgcttctc actaatgatt gtcccttgca acgtgcgatt ttgattcacc ttgataatat
    26701 aagctttatc aagtttatgt ttttttactt ctttttcagc atcttctata gaaactttag
    26761 taaacttagc atcactatga aatgtattcg cctgttgctt gaaaacctta tagatttgtt
    26821 cattcggtgc tgctacacca attttatctg gaccatcatc aaacatgtta ataatcttat
    26881 caatgttaga taggccaatc attaaggcag caatgataat cataaaaatt acaaatgatt
    26941 tagctttaat ttttttgata tatgtcaaag taaatgtcgc ccaaaactta tgcatccttg
    27001 ccaccaacct tctcaatgaa tatatcttgt aatgatggtt ctacaacttg gaatcgttta
    27061 acataacctt gatgtgctac aacttgataa atatctttgg atacgtcttc attctcaatc
    27121 gtcaactgaa gaccttgctt catgttttca ctatgaatga tgcctctaat gtgtgttaaa
    27181 tctggtagtg ttgtttctga ctcaatgaca actttcttgt taccattaga tgcacgtaca
    27241 tgattgatat caccagaaac aacaagttga cctttatcta aaatacaaac atcatcacat
    27301 aattcttcaa catgttccat acggtgagaa ctataaacga ttgtactgcc ccaatcattt
    27361 aagtctttaa ctgcttcttt taataactca acattaactg ggtctagacc actgaaaggc
    27421 tcatctaata ttagtaattc tggtttatgt aacatacttg ctaacagctg aattttttgt
    27481 tgattccctt ttgatagact atcaattcgt tttttacggt tttcagtaat atcaaaacgc
    27541 tcaagccaat acgatatttg ctgttgtatt tctgtttttg acattccctt taaagttgcc
    27601 aaatatttca attcttcttc aactgtcaat ttcccatgta aaccgcgttc ttccggtaaa
    27661 taaccaatac gattgtacat tgttttatct agttttttac cgttatacgt aatgtgtcct
    27721 tcagttggtt cacttaagcc taaaatcata cgaaatgtcg ttgttttacc tgcaccattt
    27781 cttcctagaa aacctaacat tttgcctgat tctaactcta atgacatatc atttactgcc
    27841 gtcatcttac caaaacgttt cgtaacatgc tcaattacaa gtcccatctt ttgcctccta
    27901 aaacaatatg tatttatctt aatataacat ttctattctc tataaataca atatttttaa
    27961 agtgaattta tttttaaaat ttctgaaatt gaaatattta aatagtatct tctttgcatg
    28021 ttaagtatca tgagcgttag atatgctttt ttccatgcct ttattgcctt attggtaaaa
    28081 aaaatatccc agcaacaatc tgctgagata tctatatcat tacaattgat tatctgtttc
    28141 ttccggtgtt ttcggtgttt cagatgtccc cgcttttttc ggtgtttcag atgtcactga
    28201 ttgattttta attgaagatg ggtattttgg gctttcgcca tatttatttg ccggcttact
    28261 gtcaaagcat aggaatacta tcataacaat tgttaggcct aaataaacaa aataaagaag
    28321 tactaacaaa atattaagac ccatcggcat taaggtaaaa tcactgtcat aataactatc
    28381 gataatctgt aatactatat aaaatataat actgaatact gtcataatca ttggaaataa
    28441 cattgttctt gatatatcgt gaaatcttcg aacgcacaac gctaaatttg gaataaacgt
    28501 tgccaaacta tagacaaaag tatacacaga tgtaaggata atcatcaata tactcataac
    28561 tattaatgct tcgttatctg ccgctataga aataaagaat ataaataggc ttattattag
    28621 cacacacaca gctggaacca taagtattaa atgccacaac atcatatacc aatattcact
    28681 acgtcttgat ctccccttaa aattaacata atttttccaa aataatacga atgatttcat
    28741 aaaacctact tgaggtaatt gttccattgt aatctccctt tcgttaatca tatttatatt
    28801 tttaattatt attaccgtta taatttacaa gattcattat caagccactt catttcaatc
    28861 gtcattcact ttggaaaatg aagtttatat ttcatcacta gttaaatgaa aacccgctac
    28921 aagtacgcat ctatatggag actcatttga aagtcaatgc ttcgttaact atactaaaaa
    28981 tatgtcacac tgcaatgttc atgtttaaaa gagtctcaat ctctgcaaat aaaatattcc
    29041 ataacaaagt atatacttta catttttata attattaaca atactatttt atcaaacatt
    29101 tactacaata aaaatatctt tttcattttt atttaaatta atcatataat tgcgaggaga
    29161 atattatgga tttcgttaat aatgatacaa gacaaattgc taaaaactta ttaggtgtca
    29221 aagtgattta tcaggatacc actcaaacgt atacaggcta catcgtggaa acggaagcat
    29281 acttaggttt gaatgatcgt gcggctcatg gctatggtgg taaaataaca cctaaagtta
    29341 cgtcattata taaacgtggt ggtacaattt atgcacatgt catgcatacg catttactca
    29401 ttaattttgt aacaaaatct gaaggtatac ctgaaggcgt acttatccgc gcaattgaac
    29461 cagaagacgg tttatccgct atgttccgta acagaggtaa gaaaggctac gaggtaacga
    29521 atggtccagg aaaatggact aaggcattta acattccacg ggctatcgat ggcgctacgt
    29581 taaatgactg tagattgtct attgatacta agaatcgtaa atatcctaaa gatattattg
    29641 ctagtccacg aatcggtatt ccaaataaag gtgattggac acataaatct ttacgttaca
    29701 cagtgaaagg taatccattt gtgtctcgca tgcgtaaatc agattgtatg tttcccgaag
    29761 atacttggaa ataaatgtca tctttcattg attactatca tgaaaatgaa atctatctcc
    29821 ttataagtca atcaatcagc cgtcaacatg cggatggggt gattgttttt ctttgtgtcc
    29881 atcatatttt ttgattcatc tcctcttttt gaacttgttc ttaattttaa aatataacaa
    29941 tagaattatt tataattatt aaatttagat gtattaatat tattgatatt attttcaaaa
    30001 actagaaata ttgatttatt gcatgtataa tgttaaaagt gcccttttat aacgcttaca
    30061 tataaaagct tatttaggga gagggatatt caacaagggg gatttgaaaa tgatagaact
    30121 taatgcaatt acaacattat gtttagcctg tatactttac ttacttggta aagctatcgt
    30181 taatcacgtt aattttttaa aacgcatttg tataccagca ccagtgattg gtggcttaat
    30241 ctttgctatt ttagttgcgg ctttggattc atttggcatg gtcaagatta aattagatgc
    30301 ttcattcatt caagatttct tcatgttagc attctttacg acaatcggtc ttggtgcatc
    30361 attgaaatta tttaaattag gtggcaaagt cttgctatta tactttatgt tttgtgctat
    30421 catttcagtc attcaaaaca tagttggtgt atcactagca aaagtattaa atattaaacc
    30481 gttactagga ttaacagctg gttctatgtc tatggaaggt ggtcacggta atgctgccgc
    30541 ttatggtaag acaatccaag atttaggcat tgactcagca ttgacagcag cacttgcggc
    30601 tgcaacttta ggtcttgtat ttggagggct tatcggcgga ccagttgtaa aatttttaat
    30661 caaacgttat aacttaaagc ctcaacatag tgacgataca tttaaagatt atagccaagt
    30721 tgcatataac gaacatttac atagtaaatt taatgccact gaagtattct tcattcaatt
    30781 tacaatcgtt gtattctgta tggcagttgg aagttatttc agtcatttat ttacagctca
    30841 aacagggatt aatattccaa tttacgttgg ctcattattt gtagcggtta ttgtccgaaa
    30901 tatctctgaa agttttaatt ttaatattgt agatttaaaa attactaatc aaattggcga
    30961 tgtcgcatta ggtattttct tatctcttgc gctaatgagc attcaattaa tcgaaattta
    31021 taaacttgct atacctctta ttattatcgt tttagttcaa gttgtcgtta tgattttatt
    31081 tgctgtttta attttattta gaggtttagg aaaagattat gatgctgcag taatggtagg
    31141 tggttttatc gggcatgggc ttggtgcaac gccaaatgcc atggcaaatt tagatgttat
    31201 tactaaaaaa tatggaaact cacctaaagc atatttagtt gtacctattg ttggtgcatt
    31261 cttaatcgat ttaattggtg ttatagtcat tatgggattc attcaatggt ttagttaaac
    31321 accaaactca taaataaaag aggaggcctt cgcctcctct tttatttatc ctcgatgtat
    31381 attcaagcta cgttgttcta tccatgacaa tatttccgga ctaaatacga tttgtttttg
    31441 tgttaagtcg tcaatatttt tagcatctaa catcgtcatt attgatttca tgtgttcaat
    31501 aaatgattct acataagcta ctgtatgtgc aatgccatta ttttcaactt gatttaaaaa
    31561 cggacgtgac ataccagttg cctttgcacc aagtgctaaa cttttaattg catcgagtgg
    31621 tgtacgtaaa ccaccactcg cgaaaactga aatttcgctt tgataagccg ttgtttcaag
    31681 taatgactca actgtagact gtccccatga tgataagtaa tccatatctt tatttgcacg
    31741 acgttcattt tcaatatcta caaagttagt accacctttg ccactaacat cgacatactt
    31801 aacgcctatt tgttgtaagt catgcattaa ttctttgctc ataccaaatc caacttcttt
    31861 tataatgact ggaacagata ctcgtgatac aatcgacgct atattatcta accaagtcac
    31921 aaattcacga ttcccttcag gcataactaa ttcttgagga gaattaacat ggatttgtaa
    31981 cgcttgtgcc tcaagtaatt caactgcttc caaagccttt tctactggta cgtccgcacc
    32041 aacattgcta aaaatcatgc cttcaggatt catttttcgc gcaatcgtaa atgtctcagc
    32101 catgcgtgga tttctcaatg ctgcatgtgt tgatccaact gccatcgcta agccagtttc
    32161 tcttgcaact acagctagct tttcattgat gtttttcgtc cactcgctac cacccgtcat
    32221 tgcattaata taaaccggat atgtcatcgt taagtcaggt gtctgtgatg tcaaatcgat
    32281 atcatttaca ttaattgatg ggatagaatg atgcacaaaa cgcatcttat caaaatctga
    32341 atgcattgcg tcagattggg ccattgctat ttcaacatgt tcattttttc tctgttctct
    32401 ttgaaaatca ctcatgatta aacctacctt ttcgtcattt ctaatttaaa accatttttt
    32461 acgtctgaaa aagatgatta atacgacagc tataacaaac attacagcta agcaaatata
    32521 atatctataa tgtaaatgta atgccggcat atttacaaag ttcataccat aaatcccagc
    32581 tatgaatgtt aacggtgaaa atataactga tactaatgtt aatacttgca taatactatt
    32641 cattctaaat gacgtgtatg actcaaaatt ttctcgtatt tcatttgtca tttcttgtgc
    32701 agtacgaatg atattacgtt gcttaatcaa gtggtcatcg atatgttgaa tgtatagcga
    32761 atgtttatta tctataatca aatcaccatt ttgtttcatt gtatcaatta gctcctgcat
    32821 aggaaacagt acacgtttta ctttaatcaa atccgaacgt aacttaaaga cactatccat
    32881 gaccatttta ttaaagcgat catctacatg gcggtcttca aaatgataaa cactatcttc
    32941 aagtgcatat acaaagttga aatatttatc aaccatcata tctaaaatta atatgacgac
    33001 atctgcacaa tctaattctg catctaatgt attcatatac ttatagacta ctttatttaa
    33061 tgattccaac gtttgatgat gatatgttac taatacattg tcttgtataa aaatatttag
    33121 tgctattggt gaatagtttg accccataat actatggaat actaagtatt gataatcttt
    33181 ataagattta tatttagctc gtggcatacc gttaattgca tcatccactt ctaaatcatt
    33241 aaaactaaaa tgtgctttaa accattcatt ttcttgttca ttcggttcat caaaatcata
    33301 ccaaacaata gtcgcatctt ttggtatctc tttgatatca tcaactactt taaacggttc
    33361 gtatgtagtt tgataccgta tcgttaaagc catcgatact ccccctaaat aacgaattct
    33421 ctattatttt atcatgaatt aaataacgtg tatgtcttaa tttattttag tatgatagtc
    33481 actaaggaga tggttattat cacacaactt tttacacata ctcaaaccgt aacatctgaa
    33541 ttcatcgacc ataacaatca tatgcatgat gcaaattata atatcatttt tagtgacgtc
    33601 gtgaatcgtt ttaattacag ccacggtctt tctttaaaag aacgagaaaa tttagcatat
    33661 acgctattta cactagaaga acatacgaca tacctctcag aattgtctct tggcgatgta
    33721 tttactgtta ctttatatat ttatgattac gattataagc ggttgcattt atttttaaca
    33781 ttaactaaag aagatgggac actagcatca acaaatgaag taatgatgat gggaattaat
    33841 cagcacacac gtcgttctga tgcttttcct gaatcatttt caacacaaat agcacactat
    33901 tataaaaatc aaccaactat cacttggcct gaacaattag gacataaaat agcaattcca
    33961 cacaaaggag cattaaaatg acagatgcat tacaacaaaa gattcatatc gaactactag
    34021 atttattaga tgatgttaag tttgaattaa cagaattaaa tgcacaaaaa gggttataca
    34081 ttaacggacc agcaaatcag ttacttaagc gtggcgtgca tatggcttat gttcaaggac
    34141 aaaagcaagc tatcgataat attatgacta ttgtggaaca acagcttgaa gatcaacatt
    34201 tcctagaaca ttatgataaa tttcaaaatg aggttgctca tcgcaattat gataaaacag
    34261 ctaactttgc agaacttagt gatattccac gtcaattcga taatttttta gatcaatttt
    34321 atcaaattaa agggcaatac tttatcatca cacatatcaa tacacttatt ggtgattttc
    34381 actcagaagc tcattaacaa ttagtctata taatccttgc tatattttca aaaacaaaac
    34441 ccagttgcgt tttcatgtta aatatcttct accatgaaat cgtaactggg tcatttatat
    34501 gttattagtt gttttgtgtt acatcctcat ctatcgattt ggcaatttgt ttaatagctt
    34561 tatgtgattg tctaattgga taaattggaa aatcatgtac catcttagga taatcataaa
    34621 actcaatgta ttgatgatgt tgcaacatca tttgttcgaa cagcttcata tcaggatgtg
    34681 tcatttcacg tccaccacca aacatataaa ctggtggtaa tccttctatt gtgccattaa
    34741 ttggtgatat gcgcttatct gttaatggta ggccattcgc ccattttttc ataatctcat
    34801 tgacaccaaa ctgacttaga accgcatctt gttcgattaa agcatccgaa atatctttat
    34861 tagatagtgt tgcatctaaa attggtgaga ttaaatacaa tttattcggt aatggctgtt
    34921 gattgtctaa aagagattgt acaaaggata atgccagtgc accacctgaa ccatcaccca
    34981 tgactacgac attttgatgg cctacttcag atactaattg atcataaaca cgttgtatcg
    35041 cttggaaagt gtcgtcaata tgaaactctg gtgtctttgg atagataggc agtacaacct
    35101 catataatgt acttaaagtg attttatcca acaatctcca atggaacggt gatggttgta
    35161 gtgcattgaa tccaccgtga atatataaaa ttttcttatc aatttgatgt ctgaaattaa
    35221 agcgaaagac ttgcatatca tccaatgata atttttctaa atttgcttta acatttaatg
    35281 ttgaaggctg cttatgtttt tttctatttt caatttctct tttataaaaa aatctttcaa
    35341 catcttgatc atttttaaac ataatcgagc gattgtgaag caaatattta ttgacaacgc
    35401 tattcataac acggtttcta atcaatgtct taacctacct ttatatattt tatgtatcca
    35461 atgattgtct atcccctaca ttctttgcca aaaaaagtat ataatgtaga agatattttc
    35521 tttttcactt tcaaatttaa gactacaatt gaacagtgat ttttcatcat tataacagac
    35581 aactagacat attgataagt aaagaaaaga actttatacg gaggtgcctt gtatgacaaa
    35641 tccaaatcaa cgattagaac catttgatga gacatttcaa caaccgaata ttcatcgtgg
    35701 taagcgatat ggtaagaaaa aacgttcatt ggtaagcatg attattcaaa tcattgttgt
    35761 tatattaacc gcaatcgctg gatacagcat gtggaaacaa cccattttta atattgtctt
    35821 caccaaagaa tctgtaaatt ttgatggcat taaaaatttt aacgagacaa ttggaaaaat
    35881 gcatcaatta aatattaatt tgggtaatat caatgattta caacaaggaa tcgatcgctt
    35941 aattttagtc tttaacggtt tctttatttt atgtatcgtt agcctagtca taacaatact
    36001 gacaattgta tttaatcgca cagttctaaa agtagtaaat attttagtgt taacaattat
    36061 gttagtcatt acattgtatt tcagttatat tattaaagca ctcgcacaag aaattgcaga
    36121 tgctttaaaa aaatattatt taaatgtacc accagatcaa gtgattacag aagctgacgc
    36181 tattcataac ggcatcattt tactgggctg tagcattggt ttacttgtta taagcttatt
    36241 cttccgtaat cgcatgcctc gattaaaata atgttatata gtagggattg ggacagaaat
    36301 gttattttca caaaattcat ttcgttgacc cacttcctgc tttttcttta ttaatgacct
    36361 actacatcaa tgccaaattt taatataatt tctctaatat tctacaaatc aacaaataaa
    36421 aaagtatcga taaccatata ttggcaccga tactttaaaa atcatgtccc cacaacataa
    36481 tttttgatta gtcacacttc aattaactta ttttatttat tcatgattga tactattatc
    36541 tgcatctatc ttttgagccg tagcttttgc ttttttgtta ctttgtaaaa ataagcataa
    36601 aattaaagcg atgatgctaa atatcgttgc aactataaat gcatcattaa tcccttcaac
    36661 tgttgctagt ttatttacaa attgtaatag cactttcatt gcgccttctt gaccaccata
    36721 ttgcgatgct aattcacgca tatgatcttg tacaacagga ttcgttttat ctaactcttc
    36781 cccaaaagct gataaatgtt gtgtagtttg cgttgtcatt acagtaacca agattgctgt
    36841 tcctatagaa ccagctagtt gacgcatcgt atttaagaag gcattaccat gggatgccag
    36901 tcgtcccggc aacgcattaa tagctgctgt taccattggc atcataataa atgccatacc
    36961 aaatgaacgt agtacataga tgcccatgat tgtcatatat ggcgtatcca tattcaattt
    37021 agttaattcc catgttgcat aagtcatgac agcaatacca aaaatggcta aaggttttaa
    37081 accaatcgtg tctaacaact taccagcaaa tggtcctagt agacccataa ttagagaacc
    37141 aggtaataat aacaatccag aatctaatgc tgagaaacca cgtaaatttt gtaagtaaat
    37201 tggtaataaa atcataccac catacaagct caacattaca accatattaa ttactgttgt
    37261 tagtgtaaat gttggaaatt tcaatacttc taaattcaac attggtgctt tcattcttaa
    37321 ttctcttatt acaaatagaa taataaagat aataccaatc gcaaacattg tttctatctc
    37381 aacagaaccc cagcctttgt tccccgcttc tgaaaagccg tataacaaag caccaaaacc
    37441 aatcgtacta aaaatgatgc ctgggatatc agctttaggg tttgttgtat attgatataa
    37501 cttaaaccat acaaaaccaa ctaagattgc aagaataccg ataatgaaca taccgtaaaa
    37561 catcacattc cagtggtaat tttgtacaat ataacctgat aatgttggac cgattgcagg
    37621 tgctaaaatc attgcgatac ccattgtacc catggcagca ccacgttttt caggtggata
    37681 aattgtaata ataacaattg aacctaatgg cattagtaca cctgcaccaa tggcttgtaa
    37741 tacacgtcca accatcatga ttgggaaatt cattgaaatc gcacatatta atgaaccaat
    37801 tgtaaagagt actaacgcaa ctaaaaataa ttttcgatat gaatatttat taaatagata
    37861 cgccgtaatt ggtattaaaa taccgtttac taacatgaat cccgtcatca accattgccc
    37921 tgttgacgca gaaatattaa attccgtatt aatttttggc aaagcaacat ttaataatgt
    37981 ttggtttaaa atcgcaataa acataccgaa taataatgct gctaatattt taccgcgtga
    38041 aacaccttca ccaaaaataa agtttttatg ttctttttta attttctcat ttactttata
    38101 ttcttctgag ttaggatttt tagcagcata tacttcttca tcagattcat tattatcagt
    38161 gaatgatgct tgatcttgtt cagacccctc tgtctgtttg tttagactaa cttgattctg
    38221 attgtcttta ttgtcaatac gtgcctcttc acgcgccgta tgttgtgacg attgatcagt
    38281 tgttctgtct aaatcacttg ctttaaattt agattgattt gattgacgtg tcgcaaaatg
    38341 ttgttccttt tgttggcgtt ttgcttttct tcttgatctt attaataata aattgataac
    38401 accaacaata atgagcgcta aaacaatgta gctaataatg aaggtcgtag tcatttaatg
    38461 acccccttaa tttttatgga tttttacttc agcgttcatt ccaggaacaa cttgtttaga
    38521 cggttctgaa tctaacgtaa ttttaacagg tataacttga gatactttag tatagttacc
    38581 atcactattt gatgatggca ttaatgaaaa actagcagca gtagcttttc caatactatc
    38641 aactttacct ttaacagaag ctttttgacc gtcaatagtc acatcaacat ctttaccaac
    38701 ttcaacatct ttaatatctt tttcgtcaat atttgcagtt acatataaat catctaaatt
    38761 gtaagcataa gcaattggat tgcctgcttg taccatagaa ccttccatgc catctaattt
    38821 agcaattgta cctttttgtg gcattttaag ttccatatct ttagtttcgc catcttgacc
    38881 ttgaacagta actgttgcta ctttatcacc tttatcaagt ttatcacctt gcttaacatt
    38941 tagtgatttg atttgcccag aagctggact tgctatttta atttgatcgc catttacttt
    39001 agcattatca gttgttatat aacttgttgt tttattccag aaataaaaac cagcaatccc
    39061 aattgctagc agtacaacaa ccgtaatgac attaattaat atcatctttt tcatgaaaca
    39121 tttcctccta tttgctttta aaattcactt tatttattat agagacatga taactaaaaa
    39181 aacaccaaca atttttataa gagtgttggt taaaattcac aaaggtgtaa caatatgaaa
    39241 cgacaagcca aaatagaaat tcaaaacgca cttgttgatt tgatggctga atatcctttt
    39301 caagaaatat ccactaaaat gatttgcgcg tattgtaata ttaatcgttc tactttttat
    39361 gattattaca aagataaatt tgatttattg gataccatca actcaaagca taaagaaaaa
    39421 ttccaatttc ttctaagcgc gctacatcat aatttcgaaa atattaaaca ggataagctt
    39481 aaactttata aattcttcat tattattgct aagtatatca agcgcaatga gcagtttttc
    39541 aaagacattt tagttaccta tccaatgaaa acattattca tagactatat aaatttagcg
    39601 agagattatt atcaaaaaat tatgaatgac tatagtacta cagtaaccaa taaacagctt
    39661 tatgttacat atatcatagg cggtcaagct ggggtattta ttaattggct ttcgaatggt
    39721 tgtaatgaat cacctgaaga agttgctgac attttattgg ctaatacgat taaacttcaa
    39781 aaccaataat acggtttcat tctaataaag aaatgctata aaacaactag ataaagttaa
    39841 accaaatgga cagataatag tacatatgta atggtgctat caatataaaa atgcgcttgg
    39901 gacattaagc tcatatgcat ataagatgat ttttaacatc atttttggat gataggatgt
    39961 tgcgccacga tgatgtctga attcatctaa ttcgctatca ggtatcgttt caacaatttc
    40021 attaacatat cgcgaaatat cattttgagg aattctaaca gaagtttcta ttggtagtgt
    40081 aagttgggtc atattatgat ttttata
//
LOCUS       DAKSQE010000012        38626 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.012, whole genome shotgun sequence.
ACCESSION   DAKSQE010000012 DAKSQE010000000
VERSION     DAKSQE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 38626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38626
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.012"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            121..1458
                     /locus_tag="O0V33_000488"
     CDS             121..1458
                     /locus_tag="O0V33_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCX8975170.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILKSMKQVEKHSDYDGYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLVVLTYPNYYGET
                     FNVEEVIKSLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRENIIEYLSYFQTSSPSYLIMASLESAAQFYKTYDSTLFFAKRAQLI
                     ECLENKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWH
                     HDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
     gene            1460..2077
                     /locus_tag="O0V33_000489"
     CDS             1460..2077
                     /locus_tag="O0V33_000489"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCX8975171.1"
                     /translation="MSAFITFEGPEGSGKTTVINEVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            2105..2434
                     /locus_tag="O0V33_000490"
     CDS             2105..2434
                     /locus_tag="O0V33_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCX8975172.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            2648..3574
                     /locus_tag="O0V33_000491"
     CDS             2648..3574
                     /locus_tag="O0V33_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCX8975173.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQDMSKPVAEMISTYTTQIDNALALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDSDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            3575..4378
                     /locus_tag="O0V33_000492"
     CDS             3575..4378
                     /locus_tag="O0V33_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCX8975174.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVELDDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRIADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            4395..4742
                     /gene="yabA"
                     /locus_tag="O0V33_000493"
     CDS             4395..4742
                     /gene="yabA"
                     /locus_tag="O0V33_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCX8975175.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKALAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            5016..5741
                     /locus_tag="O0V33_000494"
     CDS             5016..5741
                     /locus_tag="O0V33_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCX8975176.1"
                     /translation="MLKENERFDQLIKENFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRADRLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKEVYYG"
     gene            5734..5982
                     /locus_tag="O0V33_000495"
     CDS             5734..5982
                     /locus_tag="O0V33_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCX8975177.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            5984..6823
                     /gene="rsmI"
                     /locus_tag="O0V33_000496"
     CDS             5984..6823
                     /gene="rsmI"
                     /locus_tag="O0V33_000496"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCX8975178.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIN"
     gene            7108..9081
                     /gene="metG"
                     /locus_tag="O0V33_000497"
     CDS             7108..9081
                     /gene="metG"
                     /locus_tag="O0V33_000497"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCX8975179.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKAGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMINKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLTEP
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATEEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            9112..9885
                     /locus_tag="O0V33_000498"
     CDS             9112..9885
                     /locus_tag="O0V33_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCX8975180.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREVGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            10052..10588
                     /gene="rnmV"
                     /locus_tag="O0V33_000499"
     CDS             10052..10588
                     /gene="rnmV"
                     /locus_tag="O0V33_000499"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCX8975181.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHADLIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            10599..11492
                     /gene="rsmA"
                     /locus_tag="O0V33_000500"
     CDS             10599..11492
                     /gene="rsmA"
                     /locus_tag="O0V33_000500"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCX8975182.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEIGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVIIQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            11592..11855
                     /locus_tag="O0V33_000501"
     CDS             11592..11855
                     /locus_tag="O0V33_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCX8975183.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
     gene            12166..13014
                     /locus_tag="O0V33_000502"
     CDS             12166..13014
                     /locus_tag="O0V33_000502"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCX8975184.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFNIGASLEELALLGSKIGTDIPFCIYNKTALCTGKGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLAQKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            13028..13852
                     /gene="purR"
                     /locus_tag="O0V33_000503"
     CDS             13028..13852
                     /gene="purR"
                     /locus_tag="O0V33_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCX8975185.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            13869..14249
                     /locus_tag="O0V33_000504"
     CDS             13869..14249
                     /locus_tag="O0V33_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCX8975186.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNVDGKIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            14321..14623
                     /gene="spoVG"
                     /locus_tag="O0V33_000505"
     CDS             14321..14623
                     /gene="spoVG"
                     /locus_tag="O0V33_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HCX8975187.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDEIDEVVPDKNATSEDSEEA"
     gene            14812..14943
                     /locus_tag="O0V33_000506"
     CDS             14812..14943
                     /locus_tag="O0V33_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975188.1"
                     /translation="MRLNDARIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEV"
     gene            14966..16318
                     /gene="glmU"
                     /locus_tag="O0V33_000507"
     CDS             14966..16318
                     /gene="glmU"
                     /locus_tag="O0V33_000507"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCX8975189.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAENAMQRRTNHYHMLNGVTIIDPDSTYIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            16465..17430
                     /locus_tag="O0V33_000508"
     CDS             16465..17430
                     /locus_tag="O0V33_000508"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCX8975190.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            17580..18233
                     /locus_tag="O0V33_000509"
     CDS             17580..18233
                     /locus_tag="O0V33_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCX8975191.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            18544..19116
                     /locus_tag="O0V33_000510"
     CDS             18544..19116
                     /locus_tag="O0V33_000510"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCX8975192.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            19116..22622
                     /gene="mfd"
                     /locus_tag="O0V33_000511"
     CDS             19116..22622
                     /gene="mfd"
                     /locus_tag="O0V33_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCX8975193.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFVDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNIEEV
                     DITTASDYIITEEVISHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIDYFQKDAIIAVDEFNRIKETEESLTVESDSFISNVIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQIHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQL
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLKFLVKCIEESMRISDEA"
     gene            22612..24138
                     /locus_tag="O0V33_000512"
     CDS             22612..24138
                     /locus_tag="O0V33_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCX8975194.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGMLLFIAIFVFANN
                     IAHMMGDSHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLHAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRITGLVELLCAAVLGIIALFFYIFRFNVLTYKEITYLPFGSKLYQIKKGRR
                     "
     gene            24138..25331
                     /locus_tag="O0V33_000513"
     CDS             24138..25331
                     /locus_tag="O0V33_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCX8975195.1"
                     /translation="MEHTITIVGLGNYGIDDLPLGIYKFLMTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYEAHDQFEDVYNDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVTLNVRTHTLIT
                     QVYSSMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKITTPTYLYHDFDFATEVIDTLVDENKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVKFEKVFAEHFLNLYEKTKDKSFDEAALKQWLEK
                     GESNT"
     gene            25328..25591
                     /locus_tag="O0V33_000514"
     CDS             25328..25591
                     /locus_tag="O0V33_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCX8975196.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            25609..26001
                     /locus_tag="O0V33_000515"
     CDS             25609..26001
                     /locus_tag="O0V33_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCX8975197.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            26106..26507
                     /locus_tag="O0V33_000516"
     CDS             26106..26507
                     /locus_tag="O0V33_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCX8975198.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            26687..27982
                     /gene="tilS"
                     /locus_tag="O0V33_000517"
     CDS             26687..27982
                     /gene="tilS"
                     /locus_tag="O0V33_000517"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCX8975199.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDLSHSLNRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRNGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIEK
                     PGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVQQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKQWIIISKNGDE"
     gene            27987..28526
                     /gene="hpt"
                     /locus_tag="O0V33_000518"
     CDS             27987..28526
                     /gene="hpt"
                     /locus_tag="O0V33_000518"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCX8975200.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
     gene            28784..30877
                     /gene="ftsH"
                     /locus_tag="O0V33_000519"
     CDS             28784..30877
                     /gene="ftsH"
                     /locus_tag="O0V33_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCX8975201.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHRQA
                     PNIEKPYDPNHPDNK"
     gene            31291..32172
                     /gene="hslO"
                     /locus_tag="O0V33_000520"
     CDS             31291..32172
                     /gene="hslO"
                     /locus_tag="O0V33_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCX8975202.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
     gene            32351..33283
                     /gene="cysK"
                     /locus_tag="O0V33_000521"
     CDS             32351..33283
                     /gene="cysK"
                     /locus_tag="O0V33_000521"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCX8975203.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
     gene            33499..34302
                     /gene="folP"
                     /locus_tag="O0V33_000522"
     CDS             33499..34302
                     /gene="folP"
                     /locus_tag="O0V33_000522"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCX8975204.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVESAVTRVKAMMDEGADII
                     DVGGVSTRPGHEMITVEEELNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDII
                     NDQWAGLYDHRMFQVVAKYDAEIVLMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNFS"
     gene            34280..34645
                     /gene="folB"
                     /locus_tag="O0V33_000523"
     CDS             34280..34645
                     /gene="folB"
                     /locus_tag="O0V33_000523"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HCX8975205.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLSEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            34642..35118
                     /gene="folK"
                     /locus_tag="O0V33_000524"
     CDS             34642..35118
                     /gene="folK"
                     /locus_tag="O0V33_000524"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCX8975206.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGINVSNISSIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRVRKERWGPRTLDVDILLYG
                     EEMIDLPNLSVPHPRMNERAFVLIPLNDIAANVIEPRSKLKVKDLVFVDDSVRRYK"
     gene            35512..35604
                     /locus_tag="O0V33_000525"
     CDS             35512..35604
                     /locus_tag="O0V33_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975207.1"
                     /translation="MVNGKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            35657..37144
                     /gene="lysS"
                     /locus_tag="O0V33_000526"
     CDS             35657..37144
                     /gene="lysS"
                     /locus_tag="O0V33_000526"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCX8975208.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKKPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            37702..37816
                     /gene="rrf"
                     /locus_tag="O0V33_000527"
     rRNA            37702..37816
                     /gene="rrf"
                     /locus_tag="O0V33_000527"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            37829..37904
                     /locus_tag="O0V33_000528"
     tRNA            37829..37904
                     /locus_tag="O0V33_000528"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37862..37864,aa:Val,seq:tac)
     gene            37921..37996
                     /locus_tag="O0V33_000529"
     tRNA            37921..37996
                     /locus_tag="O0V33_000529"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37954..37956,aa:Thr,seq:tgt)
     gene            38003..38075
                     /locus_tag="O0V33_000530"
     tRNA            38003..38075
                     /locus_tag="O0V33_000530"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38036..38038,aa:Lys,seq:ttt)
     gene            38109..38183
                     /locus_tag="O0V33_000531"
     tRNA            38109..38183
                     /locus_tag="O0V33_000531"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38141..38143,aa:Gly,seq:gcc)
     gene            38191..38279
                     /locus_tag="O0V33_000532"
     tRNA            38191..38279
                     /locus_tag="O0V33_000532"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38225..38227,aa:Leu,seq:taa)
     gene            38285..38361
                     /locus_tag="O0V33_000533"
     tRNA            38285..38361
                     /locus_tag="O0V33_000533"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38319..38321,aa:Arg,seq:acg)
     gene            38382..38455
                     /locus_tag="O0V33_000534"
     tRNA            38382..38455
                     /locus_tag="O0V33_000534"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38416..38418,aa:Pro,seq:tgg)
     gene            38479..38554
                     /locus_tag="O0V33_000535"
     tRNA            38479..38554
                     /locus_tag="O0V33_000535"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38512..38514,aa:Ala,seq:tgc)
BASE COUNT        13853 a         5252 c         7813 g        11708 t
ORIGIN      
        1 atacaataga caagctattg cataagtaac actaactttt gtcaaagaag tgttactata
       61 taattaatac ttttgaaagt aactaattcc aaaacattgt aaacaaagga agcgtatatc
      121 atgaagcaac ctattttaaa taaattagaa agtttaaatc aagaagaagc gatttctttg
      181 catgttccgg gtcataaaaa tatgactatc ggtcatttat ctcaattatc aatgacaatg
      241 gataaaactg aaatacctgg attagatgat ttacatcatc ctgaagaagt cattttgaaa
      301 agtatgaagc aggtggagaa acattcagat tatgatggtt atttcttagt gaatggcacc
      361 acttcgggaa tattatctgt catccagtct ttttcacaga aaaaaggcga tatcttaatg
      421 gcaagaaatg tacataaatc tgtattacat gcgctcgata ttagccaaca agaagggcat
      481 tttattgaaa cgcatcaaag tccgttaacg aatcattata ataaagttaa tttaagccgt
      541 ttgaataatg acggtcacaa acttgttgtg ttgacttatc ctaactatta cggtgaaaca
      601 tttaatgtag aagaggttat caaatctttg caccaattaa atattcctgt actcattgac
      661 gaagcacacg gcgcgcactt tggattgcaa ggatttccag attctacatt aaattatcaa
      721 gctgactatg ttgttcaatc ttttcataaa acgttaccag ctttaacgat gggctcggta
      781 ctttatattc ataaaaatgc accttataga gaaaatatta tagaatatct aagctacttc
      841 caaacatcta gtccttcata tttgattatg gctagtttag agtcagctgc ccagttctat
      901 aaaacatatg atagtacctt gttttttgct aagagagcgc aattaatcga atgtttggag
      961 aataaaggtt ttgaaatgct tcaagttgat gatccgttga agctgttgat aaaatatgaa
     1021 ggctttacag gtcatgatat tcaaaattgg tttatgaatg cacatatcta tttagaatta
     1081 gcggacgact atcaagcatt agcgatattg ccgttatggc atcatgatga tacgtattta
     1141 tttgattcgc ttttacgtaa aattgaagat atgattttac cgaaaaaatc agtttctaaa
     1201 gttaaacaaa cacaactttt aacaactgaa ggtaactata aaccaaaacg ctttgaatat
     1261 gttacttggt gtgatttgaa aaaggcaaaa ggtaaagttt tggcgcgaca tattgtcccg
     1321 tatccgccag ggattcctat tattttcaaa ggagaaacaa taactgaaaa tatgatagaa
     1381 ttggtaaatg aatatctgga aactggaatg atagttgaag gaattaaaaa taataaaatt
     1441 ttagttgagg atgaataaaa tgtcagcttt tataactttt gagggcccag aaggctcagg
     1501 taaaacaact gtaattaatg aagtttatca tagattagta aaagattatg atgtcattat
     1561 gacaagagaa ccgggcggcg ttcctactgg tgaagaaata cgtaaaattg tattagaagg
     1621 caatgatatg gacattagaa ctgaagcgat gttatttgct gcatctagaa gagaacatct
     1681 tgtattaaag gtcataccag ctttaaaaga aggtaaggtt gtgttgtgtg atcgctatat
     1741 cgatagttca ttagcttatc aaggttatgc tagagggatt ggcgttgaag aagtaagagc
     1801 attaaatgaa tttgcaataa atggattata tccagatttg acgatttatt taaatgttag
     1861 tgctgaagta ggtcgcgaac gtattattaa aaattcaaga gatcaaaata gattagatca
     1921 agaagattta aagtttcacg aaaaagtaat tgaaggttac caagaaatca ttcataatga
     1981 atcacaacgg ttcaaaagcg ttaatgcaga tcaacctctt gaaaatgttg ttgaagacac
     2041 gtatcaaact atcatcaaat atttagaaaa gatatgatat aattgttaga agaggtgtta
     2101 taaaatgaaa atgattatag cgatcgtaca agatcaagat agtcaggaac ttgcagatca
     2161 acttgttaaa aataacttta gagcaacaaa attggcaaca acaggtgggt ttttaagagc
     2221 aggtaataca acattcttat gtggtgtcaa tgatgaccgt gtagatgaaa tattgtctgt
     2281 gattaatcaa acgtgtggta atagagaaca gttggtttca cctattacac ctatgggagg
     2341 cagtgcggat tcgtacattc catatccagt tgaagttgaa gttggcggtg ctactgtatt
     2401 tgttatgcca gttgatgcat tccatcaatt ttaattctat aatacaatca tcaattagag
     2461 atacttaaaa tagtgtatta ataagttatt aacaattttg ggttgcttga ctgcgactag
     2521 ttcagatgcc aatagatttg atttttgtgg ttctaagaat aatcacaaat catggtcgct
     2581 attgttgcag taattagttg ctctttggca acctttttat ataaaagcaa aagggagttt
     2641 gtaatgaatg gatgaacagc aacaattgac gaatgcatat cattcaaata aattatcgca
     2701 tgcctattta tttgaaggtg atgatgcaca aacgatgaaa caagttgcga ttaattttgc
     2761 aaagcttatt ttatgtcaaa cagatagtca atgtgaaaca aaggttagta catataatca
     2821 tccagacttt atgtatatat caacaactga gaatgcaatt aagaaagaac aagttgaaca
     2881 acttgtgcgt catatgaatc aacttcctat agaaagcaca aataaagtgt acatcattga
     2941 agactttgaa aagttaactg ttcaagggga aaacagtatc ttgaaatttc ttgaagaacc
     3001 accggacaat acgattgcta ttttattgtc tacaaaacct gaacaaattt tagacacaat
     3061 ccattcaagg tgtcagcatg tatatttcaa gcctattgat aaagaaaagt ttataaatag
     3121 attagttgaa caagacatgt ctaagccagt agctgaaatg attagtactt atactacgca
     3181 aatagataat gcactggctt taaatgaaga atttgattta ttagcattaa ggaaatcagt
     3241 tatacgttgg tgtgaattgt tgcttactaa taagccaatg gcacttatag gtattattga
     3301 tttattgaaa caggctaaaa ataaaaaact gcaatcttta actattgcag ctgtgaatgg
     3361 tttcttcgaa gatatcatac atacaaaggt aaatgtagag gataaacaaa tatatagtga
     3421 tttaaaaaat gatagtgatc aatatgcgca aaagttgtcg tttaatcaat taattttgat
     3481 gtttgatcaa ctgacggaag cacataagaa attgaatcaa aatgtaaatc caacgcttgt
     3541 atttgaacaa atcgtaatta agggtgtgag ttagatgcca aatgtaatag gtgttcagtt
     3601 tcaaaaagcg ggaaaattag aatattatac acctaatgat atacaagtag agttagatga
     3661 ctgggtagtt gtcgaatcta aaagaggcat agagataggt attgttaaaa atccattaat
     3721 ggatattgct gaagaggatg ttgtgttacc tcttaaaaat attattcgca ttgctgatga
     3781 caaagatatt gataaattta attgtaatga acgcgatgct gaaaatgcat taatactatg
     3841 taaagacatt gtaagagaac aaggtttgga catgcgttta gtcaattgcg aatatacatt
     3901 agataaatcg aaagttattt ttaattttac ggcggatgat cgtattgatt ttagaaaatt
     3961 agtaaaaata ttagcgcaac atttaaaaac acgtatcgag ttgagacaaa ttggtgtaag
     4021 ggatgaagcc aaattgcttg gcggtatcgg accttgtggt agatcgttat gttgttctac
     4081 atttttaggt gattttgaac cagtatcgat taagatggct aaggatcaaa atttatcatt
     4141 aaatccaact aaaatttccg gtgcatgtgg tcgtttgatg tgttgtttaa aatatgaaaa
     4201 tgactattat gaggaagtac gtgcgcaatt acctgatatc ggtgaagcaa ttgaaacgcc
     4261 tgatggtaac gggaaagtag ttgctttaaa tatattagac atttctatgc aggtgaagct
     4321 tgagggacat gaacagccac ttgaatataa attagaagaa atagaaacta tgcattaagg
     4381 aggcattatt acatttggat cgcaatgaaa tatttgaaaa aataatgcgt ttagaaatga
     4441 atgtcaatca actttcaaag gaaacttcag aattaaaggc acttgcagtt gaattagtag
     4501 aagaaaatgt agcgcttcaa cttgaaaatg ataatttgaa aaaggtgttg ggcaatgatg
     4561 aaccaactac tattgatact gcgaattcaa aaccagcaaa agctgtgaaa aagccattac
     4621 caagtaaaga taatttggct atattgtatg gagaaggatt tcatatttgt aaaggcgaat
     4681 tatttggaaa acatcgacat ggtgaagatt gtctgttctg tttagaagtt ttaagtgatt
     4741 aatcaagcac actcaaatag tgttataatt ataaatgaat atggtttgga taagtctgag
     4801 acaatgcatg tttcaggctt taattgtgta taaagttttg gtgattgcat aagagatagc
     4861 ggtactaaat gttattatta agtgtgcacg cagtatcatt agttataaaa tgtagctgtt
     4921 aaaagtcaaa aatacatcga atgtagatag gcatataata taaaaagaga tttcaattac
     4981 tcaatagaaa aaggttgtct tcataggagt taaaaatgtt aaaagagaat gaacgatttg
     5041 atcaactaat caaagaaaat tttagtatta ttcaaaatga tgatgttttt tcgttttcaa
     5101 cggatgcttt gttgttagga cattttacaa aacctagaac aaaagatata gtgttggact
     5161 tatgttcagg caatggggtg atacccttgt tattgtttgc gaaacatcca cgacatatag
     5221 aaggtgttga gattcaaaaa acacttgtcg atatggcgcg acgcacattt caattcaatg
     5281 atgttgatga atatttaaca atgcatcaca tggatttgaa aaacgttact aaagtattta
     5341 aaccttcaca atatacttta gtaacgtgta atccgcctta ttttaaagag aatcagcaac
     5401 accaacatca aaaagaagca cataagatag cgagacatga gattatgtgt acacttgaag
     5461 attgcatgat tgcagcccgt catttattaa aagaaggtgg caggctaaat atggtacatc
     5521 gtgcagatag actcatggat gtcttgtttg aaatgagaaa agtaaatatt gaacctaaga
     5581 aagtcgtttt tatatatagt aaagtaggga aatcggcaca aacgatagtt gtagaaggtc
     5641 gaaaaggtgg aaatcaaggt ttagaaatca tgcccccatt ttatatttat aatgaagatg
     5701 gtaattatag cgaagaaatg aaggaagtat attatggata gtcattttgt atatattgta
     5761 aaatgtagtg atggaagttt atatacagga tacgctaaag acgttaatgc acgtgttgaa
     5821 aaacataacc gaggtcaagg agccaaatat acgaaagtaa gacgtccggt gcatttagtt
     5881 tatcaagaaa tgtatgagac aaagtctgaa gcattgaagc gtgaatatga aattaaaact
     5941 tataccagac aaaagaaatt gcgattaatt aaggagcgat agtatggctg tattatattt
     6001 agtgggcaca ccaattggta atttagcaga tattacttat agagcagttg atgtattgaa
     6061 acgtgttgat atgattgctt gtgaagacac tagagtaact agtaagctgt gtaatcatta
     6121 tgatattcca actccattaa agtcatatca cgaacataac aaagataagc agactgcttt
     6181 tatcattgaa cagttagaat taggtcttga cgttgcgctc gtatctgatg ctggattgcc
     6241 cttaattagt gatcctggat acgaattagt agtggcagcc agagaagcta acattaaagt
     6301 agagactgtg cctggaccta atgctgggct gacggctttg atggctagtg gattaccttc
     6361 atatgtatat acatttttag gatttttgcc acgaaaagag aaagaaaaaa gtgctgtatt
     6421 agagcaacgt atgcatgaaa atagcacatt aattatatac gaatcaccgc atcgtgtgac
     6481 agatacatta aaaacaattg caaagataga tgcaacacga caagtatcac tagggcgtga
     6541 attgactaag aagttcgaac aaattgtaac tgatgatgta acacaattac aagcattgat
     6601 tcagcaaggc gatgtaccat tgaaaggcga atttgttatc ttgattgagg gtgctaaagc
     6661 gaataatgag atatcgtggt ttgatgattt atctatcaat gagcatgttg atcattatat
     6721 tcaaacttca cagatgaaac caaaacaagc tattaaaaaa gttgctgaag aacgacaact
     6781 taaaacgaat gaagtatata atatttacca tcaaataaat taatcacttt atcgattata
     6841 tgaaatttta aacgatttta taaacgcaag ctgtaatttt aaatggtaag ttatcatttt
     6901 gcattgatac tgataaaatg atgttgacta tgataaaaaa atgatgacat cgacgttttt
     6961 taatgtaaaa taaatacatt gaaagtaata aataccttaa cattgaataa gatgaaaatg
     7021 agatgacgag ataaatgttc gcgtccgttg aaatgcatag aaatcttaga tattatttga
     7081 agtgagacat tacgaggagg aacagttatg gctaaagaaa cattttatat aacaacccca
     7141 atatactatc ctagtgggaa tttacatata ggacatgcat attctacagt ggctggagat
     7201 gttattgcaa gatataagag aatgcaagga tatgatgttc gttatttgac tggaacggat
     7261 gaacacggtc aaaaaattca agaaaaagct caaaaagctg gtaagacaga aattgaatat
     7321 ttggatgaga tgattgctgg aattaaacaa ttgtgggcta agcttgaaat ttcaaatgat
     7381 gattttatca gaacaactga agaacgtcat aaacatgtcg ttgagcaagt atttgaacgt
     7441 ttattaaagc aaggtgatat ctatttaggt gaatatgaag gttggtattc tgttccggat
     7501 gaaacatact atacagagtc acaattagta gacccacaat acgaaaacgg taaaattatt
     7561 ggtggtaaaa gtccagattc tggacatgaa gttgaactag taaaagaaga aagttatttc
     7621 tttaatatta gtaaatatac agatcgttta ttagagttct atgaccaaaa tccagatttt
     7681 atacaaccac catcaagaaa aaatgaaatg attaacaact tcattaaacc aggacttgct
     7741 gatttggctg tttctcgtac atcatttaac tggggtgtcc atgttccgtc taatccaaaa
     7801 catgttgttt atgtttggat tgatgcgtta gttaactata tttcagcatt aggctattta
     7861 tcagatgatg agtcactatt taacaaatac tggccagcag atattcattt aatggctaag
     7921 gaaattgtgc gattccactc aattatttgg cctattttat tgatggcatt agacttaccg
     7981 ttacctaaaa aagtctttgc acatggttgg attttgatga aagatggaaa aatgagtaaa
     8041 tctaaaggta atgtcgtaga cccgaatatt ttaattgatc gctatggttt agatgccaca
     8101 cgttattatc taatgcgtga attaccattt ggttcagatg gcgtatttac acctgaagca
     8161 tttgttgagc gtacaaattt cgatctagca aatgacttag gaaacttagt aaaccgtacg
     8221 atttctatga ttaataagta ctttgatggc gaattaccag cgtatcaagg tccacttcat
     8281 gaattagatg aagaaatgga agctatggct ttagaaacag tgaaaagtta tactgaaagc
     8341 atggaaagtt tgcaattttc tgtggcatta tctacggtat ggaagtttat tagtagaacg
     8401 aataagtata ttgacgaaac aacgccttgg gtattagcta aggacgatag ccaaaaagat
     8461 atgttaggca atgttatggc tcacttagtt gaaaatattc gttatgcagc tgtattattg
     8521 cgtccattct taactcatgc gccgaaagag atttttgaac aattgaacat taacaatcct
     8581 caatttatgg aatttagtag tttagagcaa tatggtgtgc ttactgagcc aattatggtt
     8641 actgggcaac ctaaacctat tttcccaaga ttggatagcg aagcggaaat tgcatatatc
     8701 aaagaatcaa tgcaaccgcc tgctactgaa gaggaaaaag aagagattcc tagcaaacct
     8761 caaattgata ttaaagactt tgataaagtt gaaattaagg cagcaacgat tattgatgct
     8821 gaacatgtta agaagtcaga taagctttta aaaattcaag tagacttaga ttctgaacaa
     8881 agacaaatcg tatcaggaat tgccaaattc tatacaccag atgatattat tggtaaaaaa
     8941 gtagcagttg ttactaactt gaaaccagct aaattaatgg gacaaaaatc tgaaggtatg
     9001 atattatctg ctgaaaaaga tggtgtatta accttagtaa gtttaccaag tgcaattcca
     9061 aatggtgcag tgattaaata actgtatttt ttaaaattag gagagataat tatgttaatc
     9121 gatacacatg tccatttaaa tgatgagcaa tacgatgatg atttgagtga agtgattaca
     9181 cgtgctagag aagtaggtgt tgatcgtatg tttgtagttg gttttaacaa atcgacaatt
     9241 gaacgcgcga tgaaattaat cgatgagtat gattttttat atggcattat cggttggcat
     9301 ccagttgacg caattgattt tacagaagaa cacttggagt ggattgaatc tttagctcag
     9361 catccaaaag tgattggtat tggtgaaatg ggattagatt atcactggga taaatctcct
     9421 gcagatgttc aaaaggaagt ttttagaaag caaattgctt tagctaagcg tttgaagtta
     9481 ccaattatca ttcataaccg tgaagcaaca caagactgta tcgatatctt attggaggag
     9541 catgctgaag aggtaggcgg gattatgcat agctttagtg gttctccaga aattgcagat
     9601 attgtaacta ataagctgaa tttttatatt tcattaggtg gaccagtgac atttaaaaat
     9661 gctaaacagc ctaaagaagt tgctaagcat gtgtcaatgg agcgattgct tgttgaaacc
     9721 gatgcaccgt atctttcgcc acatccatat agagggaagc gaaatgaacc ggcgagagta
     9781 actttagtag ctgaacaaat tgctgaatta aaaggcttat cttatgaaga agtgtgcgaa
     9841 caaacaacta aaaatgcaga gaaattgttt aatttaaatt cataaagtta aaagtgagaa
     9901 agatcaccgc cataaatgta aacgatgcta tattcgttta atatgctatg gttctttctc
     9961 acttttttaa attaaaatat cgtgcatgtg caatacgtgc gatagagatg gttagagctt
    10021 tgaaattaag aattgtagga aggcgtttta aatgaaaatc aatgagttta tagttgtaga
    10081 aggacgagat gatactgagc gtgttaaacg agctgttgaa tgtgatacga ttgaaacgaa
    10141 tggtagtgcc atcaacgaac aaactttaga agtaattaga aatgctcaac aaagtcgagg
    10201 cgttattgta ttaacagatc cagatttccc aggagataaa attagaagta caattactga
    10261 acatgtcaaa ggtgttaaac atgcgtatat tgatagagaa aaagctaaaa ataaaaaagg
    10321 gaaaattggt gttgaacatg ccgacttaat tgatattaaa gaagcgttaa tgcatgttag
    10381 ttcacccttt gatgaagctt atgaatcaat tgataaatct gtgctaatag agttggggtt
    10441 aatcgttggg aaagatgcaa ggcgccgtag agaaatttta agtagaaaat tgcgaatcgg
    10501 ccattccaat ggtaagcagt tattgaaaaa gttaaatgca tttggttata ccgaagcgga
    10561 tgtaaggcaa gctttagaag atgaatgagg aagtgaaaat gttggataat aaagatattg
    10621 caacaccatc aagaacgcga gcgttgttag ataaatatgg ctttaatttt aaaaaaagtt
    10681 taggacagaa ctttttaata gatgtgaata tcattaataa tatcattgat gcaagtgata
    10741 ttgatgcaca aactggggtg attgaaattg gtccaggtat ggggtcgttg acagaacaat
    10801 tggccagaca tgctaaaaga gtattggcat ttgaaattga tcaacgatta ataccggtat
    10861 taaatgatac actatcacct tatgataatg tgacggtgat taatgaagat attttaaaag
    10921 cgaatattaa agaagctgtt gaaaatcatt tacaagattg cgaaaaaata atggttgttg
    10981 caaacctgcc gtactatatt acgacgccaa ttttattaaa tttaatgcaa caagatatac
    11041 caattgatgg ctacgtggtg atgatgcaaa aagaagtggg cgaacgctta aatgctgaaa
    11101 taggttcaaa agcatatggt tcattatcaa ttgtcgtaca atactataca gagactagta
    11161 aagtattaac ggtacctaaa tctgtattta tgccaccacc taatgttgat tcaatagttg
    11221 taaaactgat gcagagaact gaaccgttag taacagtaga taacgaggaa gcattcttta
    11281 agttagcaaa agcagcattt gcacaaagaa gaaagacaat taacaataac tatcaaaatt
    11341 attttaaaga tggtaaacaa cacaaagaag tgattataca atggttagaa caagcgggta
    11401 ttgatccaag acgtcgcggt gaaacgctat ctattcaaga ttttgctaaa ttgtatgaag
    11461 aaaagaaaaa attccctcaa ttagaaaatt aaatgattga caaagcaaag cactattgtt
    11521 aaaatttaaa ttttgtttga cgaaaacgct gcaaatatgg tattatgtaa cttgtagcga
    11581 ggtggagcaa tatgccaaaa tcaattttgg acatcaaaaa ttctattgat tgtcatgtag
    11641 gaaatcgtat tgtactgaaa gccaatggag gccgtaagaa aacaataaaa cgttctggaa
    11701 ttttaaaaga aacatatccg tcagttttca ttgttgagtt agatcaagac aaacacaact
    11761 ttgagagagt atcttataca tacactgatg tgttaactga aaatgttcaa gtttcatttg
    11821 aagaggataa tcatcacgaa tcaattgcac actaaataag acatatagag atgttagacg
    11881 tttcttagta taagaagtaa atattatgat aattatttga gtgttgggca ttatgttcaa
    11941 tactcttttt atttacaaaa tgtttaacac tgatgtttcg cttatagatt tttcagtaaa
    12001 tggataattg tatttataaa cacaaataca agtaaatact aagtaattag atggagaaaa
    12061 ttactttttt attaaaaaaa cactaaaaaa caaattaaaa tgtcaaatat taattctctt
    12121 tatgttaaaa tcatcatatt aagataacga aaagagggcg caaaaatgat atatgaaacg
    12181 gcaccagcca aaattaattt tacgctcgat acacttttta aaagaaatga tggctatcat
    12241 gagattgaaa tgataatgac aacagttgat ttaaatgatc gtttaacttt tcataaaaga
    12301 aaagatcgaa agatagttgt tgagattgaa cataattatg tgccttctaa tcataaaaat
    12361 ctcgcatatc gtgcagcgca actatttatt gagcaatatc aactaaagca aggtgtaaca
    12421 atttctatcg ataaagaaat acctgtttct gctggcttag ctggaggttc ggctgatgca
    12481 gcagcaacgt taagaggatt gaatcgactt tttaatatag gggcgagttt ggaagaattg
    12541 gctctactag gcagtaaaat cgggacagat attccgtttt gtatttataa taaaactgca
    12601 ctatgtactg gaaaaggaga aaaaatcgag tttttaaata aaccaccttc agcttgggtg
    12661 attcttgcta aaccaaactt aggcatatca tcaccagata tatttaagtt gattaattta
    12721 gataagcgtt acgacgtaca tacgaaaatg tgttatgagg ccttagaaaa tcgagattat
    12781 caacaattat gtcaaagttt gtctaatcga ttagagccaa tttctgtttc aaaacaccca
    12841 caaatcgata aattaaaaaa caatatgttg aaaagtggtg cagatggtgc gttaatgagt
    12901 ggaagcggac cgactgtgta tgggctagca caaaaagaaa gccaagcaaa aaatatttat
    12961 aatgcagtta acggttgttg taatgaagtg tacttagtta gactattagg atagaagggt
    13021 tgaaaagatg agatataaac gaagcgagag aattgttttt atgacgcaat atttgatgaa
    13081 ccatccgaat aaattgattc cattaacttt ttttgtgaaa aaatttaaac aggcgaagtc
    13141 ttcaataagt gaagatgtcc aaattataaa aaatacattc caaaaagaaa agttaggtac
    13201 agtaattact actgctggcg caagtggtgg tgttacgtat aaaccaatga tgagtaaaga
    13261 agaggcgact gaagttgtta atgaggtcat tactctatta gaagagaaag aacgtttgtt
    13321 acctggcggg tatttatttt tatcagattt ggtaggtaat ccatcgctac taaacaaagt
    13381 tggtaagtta attgccagta tttacatgga agaaaaatta gatgctgttg ttaccattgc
    13441 gacaaaaggt atttcattgg caaatgcggt tgctaatatt ttaaatttac cagtagtagt
    13501 gattagaaaa gacaacaagg tgactgaagg ttctacagtt tcaattaatt acgtttcagg
    13561 atcttcaaga aaaatagaga caatggtact ttcgaagaga actttagcag aaaattcaaa
    13621 tgttttagtt gtcgatgatt ttatgagggc tggtggctct atcaatggtg ttatgaattt
    13681 aatgaatgag tttaaagccc atgtaaaagg ggtatcagta cttgtagaat caaaagaagt
    13741 taaacaaaga ttgattgaag attatacttc cttagtgaaa ttatctgatg tagatgaata
    13801 taatcaagag tttaacgtag aacctggcaa cagtttatct aagttttcat aaaaggagtt
    13861 ttagtattat gaaaatcatt aacacaacaa gattaccgga agcacttgga ccatattcgc
    13921 atgcaacagt tgtgaatggt atggtttata cttctggtca aattccattg aatgttgatg
    13981 ggaaaatcgt aagcgctgat gttcaagcac aaacaaaaca agttttagaa aatttaaagg
    14041 ttgttttgga agaagcagga tctgatttga attctgttgc gaaagcgacc attttcatta
    14101 aagatatgaa tgatttccaa aaaataaatg aagtgtatgg tcaatatttt aatgaacaca
    14161 agccagcgcg tagttgtgta gaggttgcgc gtttgccaaa agatgtgaaa gtagaaattg
    14221 aattagtaag taaaattaag gaattataat tttcgattaa tatgtttaat caagcttcta
    14281 aataaaacag agagagatat actatagggg ggctcactac atgaaagtga cagatgtaag
    14341 acttagaaaa atacaaacag atggacgaat gaaagcactc gtttccatta cattagatga
    14401 agctttcgta attcatgatt tacgtgtaat tgaaggaaac tctggcttgt tcgttgcaat
    14461 gccaagtaaa cgtacaccag atggtgaatt ccgcgacatc gcgcatccta ttaattcaga
    14521 tatgagacaa gaaattcaag atgcagtgat gaaagtatat gatgaaatag atgaagtagt
    14581 accagataaa aacgctacat cagaagattc agaagaagct taatcaattt tatatttagc
    14641 gatgtaatac atttgcaata agttgatttg atactgtcga taaagcataa agctttgtcg
    14701 gcagtttttt tagtttgtat taatattaat tttatgaaat gaaaggctaa taaatatata
    14761 tgttaacaga ttatgatgat atgaaaatta ttgatacatt gctgataaaa attgcgtttg
    14821 aatgatgctc gtatttttga agtaaggaaa aagttgtttt taaaattaca acgaattaaa
    14881 aacaatgcct tttatatgtt gaaagagtat tgcagattaa attataataa tgacgaagtg
    14941 taaaatttaa tgggggttaa tgttcatgcg aagacacgcg ataattttgg cagcaggtaa
    15001 aggcacaaga atgaaatcta aaaagtataa agtgctacac gaggttgctg gaaaacctat
    15061 ggtcgaacat gtattggaaa gtgtgaaagg ctctggtgtc gatcaagttg taaccatcgt
    15121 aggacatggt gctgaaagtg taaaaggaca tttaggcgag cgttctttat acagttttca
    15181 agaggaacaa ctcggtactg cgcatgcagt gcaaatggcg aaatcacact tagaagacaa
    15241 ggaaggtacg acgatcgttg tatgtggtga cacaccgctc atcacaaagg aaacattaga
    15301 aacattgatt gcgcatcacg aggatgctaa tgctcaagca actgtattat ctgcatcgat
    15361 tcaacaacca tatggatacg gaagaatcgt tcgaaatgcg tcaggtcgtt tagaacgcat
    15421 agttgaagag aaagatgcaa cgcaagctga aaaggatatt aatgaaatta gttcaggtat
    15481 ttttgcgttt aataataaaa cgttgtttga aaaattaaca caagtgaaaa atgataatgc
    15541 gcaaggtgaa tattacctcc ctgatgtatt gtcgttaatt ttaaatgatg gcggcatcgt
    15601 agaagtctat cgtaccaatg atgttgaaga aatcatgggt gtaaatgatc gtgtaatgct
    15661 tagtcaggct gagaatgcga tgcaacgtcg tacgaatcat tatcacatgc taaatggtgt
    15721 gacaatcatc gatcctgaca gcacttatat tggtccagac gttacaattg gtagtgatac
    15781 agtcattgaa ccaggcgtac gaattaatgg tcgtacagaa attggcgaag atgttgttat
    15841 tggtcagtac tctgaaatta acaatagtac gattgaaaat ggtgcatgta ttcaacagtc
    15901 tgttgttaat gatgctagcg taggagcgaa tactaaggtc ggaccgtttg cgcaattgag
    15961 accaggcgcg caattaggtg cagatgttaa ggttggaaat tttgtagaaa ttaaaaaagc
    16021 agatcttaaa gatggtgcca aggtttcaca tttaagttat attggcgatg ctgtaattgg
    16081 cgaacgtact aatattggtt gcggaacgat tacagttaac tatgatggtg aaaataaatt
    16141 taaaactatc gtcggcaaag attcatttgt aggttgcaat gttaatttag tagcacctgt
    16201 aacaattggt gatgatgtat tggtggcagc tggttccaca atcacagatg acgtaccaaa
    16261 tgacagttta gctgtggcaa gagcaagaca aacaacaaaa gaaggatata ggaaataatc
    16321 atttacgtat ttaaaatggc taggataaaa ggataatcct atgtaatatt aatgtaatct
    16381 ttatgattta atgattcgca tagtaatgga gttacatttt atatataata gtaattgcgt
    16441 aagtaaataa ttggaggact ataaatgtta aataatgaat ataagaattc gtcattaaag
    16501 attttttcat tgaaaggaaa cgaagcatta gcgcaagaag ttgctgacca agtaggaatt
    16561 gaactaggta aatgttcagt taaacgtttt agtgatggag aaattcaaat taatatcgaa
    16621 gagagtattc gtggttgtga cgtatttatt attcaaccaa catcatatcc tgtgaatcta
    16681 catttaatgg aattattaat tatgattgat gcttgtaaac gtgcttctgc agcaacaatc
    16741 aatattgtag tgccatatta tggatatgca agacaagata gaaaagcccg tagccgtgag
    16801 ccaatcactg ctaaattagt tgcaaactta atcgaaacag ctggcgcaac tcgtatgatt
    16861 gcgttagact tacatgcacc acaaattcaa ggattctttg atattccaat tgaccactta
    16921 atgggtgtgc caattcttgc taaacatttc aaagatgatc cgaatattaa cccagaagaa
    16981 tgtgtcgttg tttcaccaga ccatggcggt gttacacgtg cacgtaaatt agctgacatt
    17041 ttaaaaactc caattgcaat tatagataaa cgtcgtccta gaccaaatgt tgctgaagtg
    17101 atgaacattg ttggtgagat tgaaggacgt acggcaatta ttattgacga tattattgat
    17161 acagcaggta caatcacttt agctgcacaa gcattaaaag ataaaggtgc taaagaagta
    17221 tatgcttgtt gtacacaccc tgttttatca ggaccggcta aagaacgtat cgaaaattct
    17281 gctataaaag aattaatcgt aacaaactca attcatttag atgaagatcg caaaccatct
    17341 aacactaaag aattatctgt tgctggttta atcgcacaag ctatcattcg tgtatacgaa
    17401 agagaatcag ttagcgtatt atttgactaa tatttaaaag gcgtttgacg aacatattcc
    17461 aaacgtgtat aatagtttcg ttcgtgatta tacgaataaa taaacacttg caagcaacga
    17521 tgatgttgat gggtaagtga ggtgctcgtt ttgagcaaaa atgaaaggtg gaaatgagaa
    17581 tggcttcatt aaagtcaatc atccgtcaag gtaaacaaac acgttcagat cttaaacaat
    17641 taagaaaatc tggtaaagta ccagcagtag tatacggtta cggtactaaa aacgtgtcag
    17701 ttaaagttga tgaagtagaa ttcatcaaag ttatccgtga agtaggtcgt aacggtgtta
    17761 tcgaattagg cgttggttct aaaactatca aagttatggt tgcagactac caattcgatc
    17821 cacttaaaaa ccaaattact cacattgact tcttagcaat caatatgagt gaagaacgta
    17881 ctgttgaagt accagttcaa ttagttggtg aagcagtagg cgctaaagaa ggcggcgtag
    17941 ttgaacaacc attattcaac ttagaagtaa ctgctactcc agacaatatt ccagaagcaa
    18001 tcgaagtaga cattactgaa ttaaacatta acgacagctt aactgttgct gatgttaaag
    18061 taactggcga cttcaaaatc gaaaacgatt cagctgaatc agtagtaaca gtagttgctc
    18121 caactgaaga accaactgaa gaagaaatcg aagctatgga aggcgaacaa caaactgaag
    18181 aaccagaagt tgttggcgaa agcaaagaag acgaagagaa aactgaagag taagtttaac
    18241 tgattaccat aaagttttta tactttgtta aacaagcact gtgcttattt taatataagc
    18301 atggtgcttt ttgtgttatt ataaagctta attaaacttt attgctttgt actaaagtta
    18361 aattaatttt agtgagtaaa agacattaaa ctcaacaatg atacatcata aaaattttaa
    18421 tgtacttgat tttaaaatac atacttacta atctaaagaa taatgataat tgatggcaat
    18481 ggcggaaaat agatgttgtc attataataa taaatgaaac aattatgttg gaggtaaaca
    18541 cgcatgaaat gtattgtagg tctaggtaat ataggtaaac gttttgaact tacaagacat
    18601 aatatcggct ttgaagtcgt tgattatatt ttagagaaaa ataatttttc attagataaa
    18661 caaaagttta aaggtgcata tacaattgaa cgaatgaacg gagataaagt gttattcatc
    18721 gaaccaatga caatgatgaa tttgtcaggt gaagcagttg caccgattat ggattattac
    18781 aatgttaatc cagaagattt aattgtctta tatgatgatt tggatttaga acaaggacaa
    18841 gttcgcttaa gacaaaaagg aagtgcgggc ggtcacaatg gtatgaaatc aattattaaa
    18901 atgcttggta cagaccaatt taaacgtatt cgtattggtg tgggaagacc aacgaatggt
    18961 atgacggtac ctgattatgt tttacaacgc ttttcaaatg atgaaatggt aacgatggaa
    19021 aaagttatcg aacacgcagc acgcgcaatt gaaaagtttg ttgaaacatc acgatttgac
    19081 catgttatga atgaatttaa tggtgaagtg aaataatgac aatattgaca acgcttataa
    19141 aagaagataa tcattttcaa gaccttaatc aggtatttgg acaagcaaac acactagtaa
    19201 ctggtctttc cccgtcagct aaagtgacga tgattgctga aaaatatgca caaagtaatc
    19261 aacagttatt attaattacc aataatttat accaagcaga taaattagaa acagatttac
    19321 ttcaatttgt agatgctgaa gaattgtata agtatcctgt gcaagatatt atgaccgaag
    19381 agttttcaac acaaagccct caactgatga gtgaacgtat tagaacttta actgcgttag
    19441 ctcaaggtaa gaaagggtta tttatcgttc ctttaaatgg tttgaaaaag tggttaactc
    19501 ctgttgaaat gtggcaaaat caccaaatga cattgcgtgt tggtgaggat atcgatgtgg
    19561 accaatttct taacaaatta gttaatatgg ggtacaagcg ggaatctgtg gtatcgcata
    19621 ttggtgaatt ctcattgcga ggaggtatta tcgatatctt tccgctaatt ggggaaccaa
    19681 tcagaattga gctatttgat accgaaattg attctattcg ggattttgat gttgaaacgc
    19741 agcgttccaa agataatatt gaagaagtcg atatcacaac tgcaagtgat tatatcatta
    19801 ctgaagaagt aatcagccat cttaaagaag agttaaaaac tgcatatgaa aatacaagac
    19861 ccaaaataga taaatcagtg cgcaatgatc tgaaagaaac gtatgaaagc tttaaattat
    19921 tcgaaagtac atactttgat catcaaatac tacgtcgttt agtagcgttt atgtatgaaa
    19981 caccttcgac aattattgat tatttccaaa aagatgcaat cattgcggtt gatgaattta
    20041 atcgtattaa agaaactgaa gaaagtttaa cagtagagtc tgattcgttt attagcaacg
    20101 ttattgaaag tggtaatgga tttataggac aaagttttat aaaatatgat gattttgaaa
    20161 cattgattga aggctatcct gtcacttatt tttcattatt cgctacaaca atgccgataa
    20221 aactaaatca tattattaaa ttttcatgta aacctgtcca acaattttat gggcaatatg
    20281 acattatgcg ttctgaattt caacgatatg ttaatcaaaa ttatcatatc gtggttttgg
    20341 tcgaaaccga aactaaagtt gaacgtatgc aagcgatgtt aagtgaaatg catattccat
    20401 caataacaaa attgcatcgc tcaatgtcat cggggcaagc agtgattatt gaaggcagtt
    20461 tatctgaagg atttgaacta cctgatatgg gattagttgt cattactgaa cgtgagcttt
    20521 ttaaatcaaa acagaaaaag caacgaaaac gtacgaaagc tatctcaaat gctgaaaaaa
    20581 ttaagtctta ccaagattta aatgtgggag attatattgt tcatgtgcat catggtgttg
    20641 gtagatattt aggtgttgag acgctcgaag tagggcaaat acatcgtgat tatattaaat
    20701 tgcaatataa aggtacggat caactatttg ttccagtaga tcaaatggat caagttcaaa
    20761 aatatgtagc ttcggaagat aagacgccaa aattaaataa actcggtggc agtgaatgga
    20821 aaaaaacaaa agctaaagtt caacaaagtg ttgaagatat tgctgaagag ttgattgatt
    20881 tatataaaga aagagaaatg gcagaaggtt atcaatatgg ggaagacaca gctgagcaaa
    20941 caacatttga attagatttt ccatatgaac ttacgcctga ccaagcgaaa tctatcgatg
    21001 aaattaaaga tgacatgcaa aaatcgcgtc caatggatcg cttgctatgt ggtgatgttg
    21061 gttatggtaa aactgaagtt gcagtgagag cagcattcaa agctgtaatg gaaggaaagc
    21121 aggttgcatt tttagttcct acaactattt tagctcagca acattatgag acgttaattg
    21181 agcgtatgca agattttcct gttgaaattc aattaatgag tcgttttaga acgccaaaag
    21241 agataaaaca aactaaggaa ggacttaaaa ctgggtttgt tgacatagtt gttggtacac
    21301 acaaattact tagtaaagat atacagtata aagatttagg gctgttgatt gtagatgaag
    21361 aacaacgatt tggtgtacgc cataaagagc gtattaaaac attaaaacat aatgtagatg
    21421 tactaacatt gactgcaacc ccaataccta gaacattgca tatgagtatg ctaggtgtgc
    21481 gcgatttgtc agtgattgaa acgccgccag aaaatcgttt cccagttcaa acatatgtat
    21541 tagaacagaa catgagtttt atcaaagaag ctttagaaag agaactatcc cgtgatggcc
    21601 aagtatttta tctttataat aaagtgcaat ccatttatga aaaacgagaa caactccaga
    21661 tgttaatgcc agatgctaac attgcagttg ctcatggaca aatgacagag cgcgatttag
    21721 aagaaacgat gttaagtttt atcaataatg aatatgatat tttagtaacg acgacgatta
    21781 ttgaaacagg tgtcgatgtc ccaaatgcaa atactttgat cattgaagat gcagatcgct
    21841 ttggattgag tcagttgtat caattaagag gtcgtgttgg tcgttcaagt cgtattggtt
    21901 atgcatactt cttacatcca gcaaataagg tactaactga gactgcagaa gatcgattac
    21961 aagcgattaa agaatttacg gagttaggct caggatttaa gattgcgatg cgtgatttga
    22021 acattcgtgg tgctggtaat ttgttaggta aacaacagca cggctttatt gatacagttg
    22081 gatttgattt gtacagtcaa atgttagaag aagctgtaaa tgaaaaacgt ggtattaagg
    22141 aaccagaatc tgaggtgcca gaagtcgaag ttgatttaaa cttggatgca tatttgccaa
    22201 cagaatatat tgcaaatgaa caagctaaaa ttgaaattta taaaaagcta agaaaaactg
    22261 aaacatttga tcaaattatc gacataaaag atgaattaat tgatcgtttc aatgattatc
    22321 ctgttgaagt agcacgtttg cttgatatag tggaaataaa agtacacgca ttacattcag
    22381 gtatcacgtt gattaaagat aaagggaaaa taattgatat tcatttatct gtaaaagcca
    22441 ctgaaaatat tgatggcgaa gtgctgttca aagcaacaca acctttaggt agaacaatga
    22501 aggttggtgt tcaaaataat gcaatgacaa ttactttaac gaaacaaaat caatggcttg
    22561 atagtttgaa gtttttagtt aagtgcattg aagaaagtat gagaatcagt gatgaagcat
    22621 aaagaagcat ttaatggcgt tgtcgtgtta actgctgcat taattgtcat taaaattctg
    22681 agtgctgtat atcgaattcc atatcaaaat atattaggcg atacaggttt gtatgcatat
    22741 caacaagtgt atccaattgt agcattagga atgatattat cgatgaatgc cattcctagt
    22801 gcaattacac aaaatatagg gaagtatcat agtgacgaag catatgcaaa agcagtcgct
    22861 tatatacaat tagttggtat gttattattt attgctattt ttgtgtttgc gaacaatatt
    22921 gcacatatga tgggtgatag ccatttaaca cctatgattc aagctgcaag tttaagcttt
    22981 atatttatag gtatgcttgg cgtgttaaga ggttattatc aatctgcaaa taatatgaca
    23041 gttccggcta tttcccaggt tatagaacaa gttatacgag taggtattat cattgttact
    23101 attgttattt ttgtagatag aggttggacg atatatgaag cgggtacaat tgctatttta
    23161 gcatcaacga taggtttttt aggttcttca atttatttag tagcgcaccg accttttaag
    23221 tttaaaatgg taaataacac tgcaaagatt gtttggaaac agttcgcact ttcggttttg
    23281 attttcgcta tcagtcaatt aatcgtaatt ttatggcaag tgattgatag tgttactatt
    23341 attaagtcac ttcaagcgat acgcgtgcca ttcgatgttg ctataactga aaaaggagtc
    23401 tatgaccgtg gtgcatcatt tattcagatg ggattgattg taactacaac atttagtttt
    23461 gcgctcattc ctctgttaag tgacgcaatc aaaatgaata atcaggtact tatgaatcgt
    23521 tatgcaaatg cgtcattaaa gattacgatt ttaataagta cagcagcggg aataggatta
    23581 attaatttat tgcctttaat gaacggtgta ttttttaaga cgaatgattt aaccttaacg
    23641 ttaagcgttt atatgattac ggttatttgt gtatcgttaa ttatgatgga tatggcatta
    23701 ttacaagctc aacatgctgt gagaccaatt tttgtcggta tgacggcagg attggttatt
    23761 aaatttatac ttaatatcat tttgattcgt ttaagtggca ttattggtgc gagcattagt
    23821 actgttgtat cattaattat attcggtacg attatccata ttgctgtcac gagaaaatac
    23881 catttacatg cgatgagacg attttttatc aatgttgttt taggtatggt atttatgtcg
    23941 attgttgttc aatgcgtgtt aaacatagtg acaacacacg gtagaatcac tggactcgtt
    24001 gaattattat gtgcagcagt attaggtatc attgcattgt ttttctatat ttttagattt
    24061 aatgttttga catataaaga gataacttat ttaccatttg gttcaaagtt gtatcaaatt
    24121 aagaaaggaa gacgttgatg gaacatacca ttacgattgt tggcttagga aactatggca
    24181 ttgatgattt gccgctaggg atatataaat ttttaatgac acaagataag gtttatgcaa
    24241 gaacgttaga tcatccagtt atagaatcgt tgcaagatga attaacattt cagagttttg
    24301 accatgttta tgaagcacat gaccaatttg aagatgtcta taatgatatt gtggcgcaat
    24361 tggttgaagc tgctaatgaa aaagatattg tctatgcggt tccgggtcat cctagagttg
    24421 ctgagacaac tacagtgaaa ttactggctt tagcaaagga caatactgat atagatgtga
    24481 aagttttagg tgggaaaagc tttattgatg atgtgtttga agcagttaat gtagatccaa
    24541 atgatggctt cacactgtta gatgcgacat cattacaaga agtaacactt aatgttagaa
    24601 cgcatacatt gattacgcaa gtttatagtt caatggttgc tgctaatttg aaaatcactt
    24661 taatggaacg atatcctgat gattatcctg ttcaaattgt cactggtgca cgaagcgatg
    24721 gtgcggataa cgttgtgaca tgcccattat atgaattgga tcatgatgaa aatgcattca
    24781 ataatttgac gagtgtattc gtaccaaaaa tcacaacacc gacatatttg tatcatgact
    24841 ttgattttgc aacggaagtg attgatactt tagttgatga aaataaaggt tgtccatggg
    24901 ataaagtgca aacgcatgaa acacttaagc gttatttact tgaagaaaca ttcgaattgt
    24961 tcgaagctat tgacaatgaa gatgattggc atatgattga agaactagga gatattttat
    25021 tacaagtgtt attgcatact agtattggta aaaaagaagg gtatatcgac attaaagaag
    25081 tgattacaag tcttaatgct aaaatgattc gtagacaccc acacatattt ggtgatgcta
    25141 atgctgaaac tatcgatgac ttaaaagaaa tttggtctaa ggcgaaagat gctgaaggta
    25201 aacagccaag agttaaattt gaaaaagtat ttgcagagca ttttttaaat ttatatgaga
    25261 agacgaagga taagtcattt gatgaggccg cgttaaagca gtggctagaa aaaggggaga
    25321 gtaatacatg agattagata aatatttaaa agtatcacgg ttaataaagc gacgtacgct
    25381 agcaaaagag gtaagtgatc aaggtagaat tacaataaat ggtaatgttg ctaaagctgg
    25441 atcggatgtt aaagttgaag atgtgctgac gattcgcttt ggtcaaaaat tagtaacagt
    25501 taaagtaact gcattaaatg aacatgcatc taaagataac gcgaagggca tgtatgaaat
    25561 cattgaagag cgtcgacttg aagaagcgta aattggaggt gacaagcaat gaaaaataaa
    25621 gtagaacata tagaaaatca gtacacgtcg caagagaaca agaaaaaaca acgtcaaaaa
    25681 atgaaaatgc gtgttgttcg taggcgtatt acagtatttg cgggtgtatt acttgcgata
    25741 attgttgttt tatcaatctt gcttgttgtc caaaaacatc gcaatgatat tgatgcacag
    25801 gagcgaaaag cgaaagaagc acagtttcaa aagcaacaaa atgaagaaat tgcgttaaaa
    25861 gaaaagttga ataatctgaa tgacaaagat tatattgaaa aaattgcgcg tgatgattat
    25921 tacttaagca acaaaggtga agtgattttt aggttgccag aagacaaaga ttcgtctagc
    25981 tcaaaatctt cgaaaaaata aatccaaatt gattcaaaat tatccgagta tagacattgt
    26041 gaaaaaatcc aaacaaggat ataataaggg aaaatcgaat caaatcggga ggatttattt
    26101 aacatatgtc aatcgaagtt ggaaataagc ttaaaggtaa agtcactggt attaaaaagt
    26161 ttggtgcatt cgtagaatta cctgaaggaa aaagtggttt agttcacatt agtgaagtcg
    26221 cagataatta tgttgaaaac gtagaagagc acctttctgt tggtgatgaa gtagacgtaa
    26281 aagtattatc tattgctgat gatggaaaaa ttagtctttc aataaagaaa gctaaagacc
    26341 gtccacgtag acaacatacg agtaaaccaa gtcatcaaaa accagtgcaa aaagccgaag
    26401 attttgaaaa gaaattaagc aatttcttaa aagatagtga agataaatta acttcaatca
    26461 aacgtcaaac agaatctaga cgcggtggca aaggttctag acgttaatta aaataaataa
    26521 agactgtttc gataaggaat atatttagaa tgatgcgtat cgaataatcg attgcagcgt
    26581 tagacaatct aagactgttt cttaaataag gagcagtctc ttttatttgt aatgatataa
    26641 ctaagactta taccattttt gaaaattgta aaagtgaggt gatgttatgc agttaaatag
    26701 taatggttgg catgttgatg accatattgt tgtcgctgtt tctacaggta ttgatagtat
    26761 gtgtttattg tatcaactac taaatgatta taaagatagt tatagaaaac taacatgttt
    26821 acatgtcaat catggcgtta ggtcagcttc aatcgaggaa gccagatttt tagaagcata
    26881 ctgcgaacgt catcacatcg atttacatat caaaaagttg gatttgtcgc atagtctcaa
    26941 tcgaaataac agcattcaga atgaagctcg aattaaacgt tacgaatggt ttgatgaaat
    27001 gatgaatgta ttagaagcgg atgtattgct aacggcgcat catttggacg atcaattaga
    27061 aactattatg tatcgcattt ttaatgggaa atcaacgcgt aataaactag gatttgatga
    27121 gttgtcgaag cgaaatggtt atcagattta tcgaccactt ttagctgtct ctaaaaaaga
    27181 aataaaacaa ttccaagaga gatatcatat accatatttt gaagatgaat ctaataaaga
    27241 taacaaatat gttagaaatg atattcgtaa tagaattatt ccagctattg atgaaaataa
    27301 tcaacttaaa gtatcgcatt tattaaaatt aaaacaatgg catgatgaac aatatgatat
    27361 tttgcaatat tcagctaaac aatttattca agaatttgtg aagtttgatg aacagtcaaa
    27421 atatttagag gtttctaggc aagcttttaa taacttgcca aactcattaa agatggttgt
    27481 gttggattgc ctattatcaa agtattatga gttgtttaat attagtgcta aaacatacga
    27541 agagtggttt aaacaattta gtagtaagaa agcacaattc agtattaatc tcacggataa
    27601 atggataatt caaatcgcat atggtaaatt aataataatg gctaaaaata atggcgatac
    27661 atattttaga gttcaaacta ttgaaaagcc aggtaattat atttttaaca aatatcgatt
    27721 agagatacat tctaatttac caaaatgttt atttccactt acagtgagaa cacgacaaag
    27781 tggcgataca tttaaactga atgggcgcga tggttataag aaagtaaatc gcctgtttat
    27841 agattgtaaa gtgcaacagt gggttcggga tcaaatgcca atcgtattgg ataaacaaca
    27901 gcgcattatt gcggtaggag atttatatca acaacaaaca ataaaacaat ggattataat
    27961 tagtaaaaat ggagatgaat agcgttatgc ataatgattt gaaagaagta ttgttaactg
    28021 aagaagatat tcaaaatatc tgtaaggaat tgggagcaca attaacaaag gattatcaag
    28081 gtaaaccatt agtatgcgtg ggtatcttaa aaggctcagc aatgtttatg tcagatttaa
    28141 ttaaacgaat tgatacccat ttatcaattg atttcatgga tgtttctagt tatcacggag
    28201 gcactgagtc aactggtgaa gttcaaatca ttaaagattt aggttcttct attgaaaata
    28261 aagacgtatt aattattgaa gatatcttag agactggtac tacacttaag tcaattactg
    28321 aattattaca atctagaaaa gttaattcat tagaaatagt tactttatta gataaaccaa
    28381 accgtcgtaa agcggacatt gaagctaagt atgtaggtaa aaaaatacca gatgaatttg
    28441 ttgttggtta cggtttagat tatcgtgaat tataccgaaa cttaccatat atcggtacgt
    28501 taaaacctga agtgtattca aattaatttt ttaatcgatt tcagttatta ttactatgcg
    28561 tttgagaaat aatagtgtag actcaaaaat atgaaaaatg tatttcatat atatttaatt
    28621 ttagacaaga catatgtctt aaaaagttga aaaatataga gattgataaa actaacacag
    28681 ggtgtgaatg acattgatgt taagctcaat tactagctta taaaacatgt catatgttac
    28741 aatttttgtt agttttatta tgggaagtag gaggaaatga cgcatgcaga aagcttttcg
    28801 caatgtgcta gttatcgtaa taataggcgt tattattttt ggtctatttt catatttaaa
    28861 cggtaatgga aatatgccga aacagcttac atataatcaa tttactgaga agttggaaaa
    28921 aggtgacctt aaaactttag aaatccaacc acaacaaaat gtctatatgg taagtggtaa
    28981 aacgaaaaat gatgaagact attcatcaac tattttatat aacaacgaaa aagaattaca
    29041 aaaaattact gatgctgcta aaaagcaaaa cggtgtaaaa ttaacgatta aagaagaaga
    29101 aaaacaaagt gtctttgtga gtatactttc aacattaatt ccagttgtag tcatagcgtt
    29161 attatttatt ttcttcctaa gccaagcaca aggtggcggt agtggcggtc gtatgatgaa
    29221 ctttggtaaa tctaaagcaa aaatgtacga taataataaa cgtcgtgttc gtttctctga
    29281 tgtagcaggg gcagatgaag aaaaacaaga attaattgaa attgttgatt tcttgaaaga
    29341 caataaaaaa ttcaaagaaa tgggatctag gattcctaaa ggtgtcttac ttgttggacc
    29401 tccaggtact ggtaaaacat tacttgctag agcggttgca ggtgaagcag gcgcaccatt
    29461 cttctctatt agtggttcag actttgtaga gatgtttgtt ggtgttggtg cgagccgtgt
    29521 tcgtgactta ttcgataatg ctaagaaaaa cgcgccttgt atcatcttta tcgatgagat
    29581 tgatgctgtt ggtcgtcaac gtggtgcagg tgttggtggc ggtcatgatg aacgtgaaca
    29641 aaccctaaac caattattag ttgaaatgga tggattcggt gaaaatgaag gtatcattat
    29701 gatagctgct acaaaccgtc ctgatatcct tgacccagcc ttattacgtc caggtcgttt
    29761 tgatagacaa attcaagttg gtcgtccaga tgtgaaaggc cgtgaagcaa ttcttcatgt
    29821 tcatgctaaa aataaaccac ttgatgaaac ggttgattta aaagcgattt cacaacgtac
    29881 acctggtttc tcaggtgctg atttagaaaa cttattaaat gaagcatctt taattgctgt
    29941 acgtgaaggt aaaaagaaaa ttgacatgag agatatcgaa gaggcaacgg atagagttat
    30001 agccggacct gctaagaaat ctcgagttat ttctaagaaa gaacgtaata ttgttgctca
    30061 tcacgaagct ggtcatacaa ttatcggtat ggtacttgat gaggcagaag tagtgcataa
    30121 agttactatc gttccacgtg gacaagctgg tggttatgca atgatgctac ctaaacaaga
    30181 tcgtttctta atgactgaac aagagttatt agataaaatc tgtggtttac ttggtggacg
    30241 tgtatcagaa gatattaact ttaacgaagt atcaacaggt gcttcaaatg acttcgaacg
    30301 tgcaacacaa atcgcacgct caatggttac gcaatatggt atgagtaaaa aattagggcc
    30361 attacagttc ggtcatagca atggtcaagt attcttaggt aaagatatgc aaggtgagcc
    30421 taattattca agccaaatcg catatgaaat tgataaagaa gttcaacgaa tcgttaaaga
    30481 acaatacgaa cgttgtaaac aaattttatt agagcacaaa gaacaattaa ttttaattgc
    30541 tgaaacatta ttaacagaag aaacattagt tgctgaacaa attcaatcat tattctacga
    30601 aggtaaatta cctgaaattg attatgatgc agctaaagtt gttaaagatg aagattctga
    30661 atttaatgat ggtaaattcg gtaaatctta tgaagagatt cgtaaagagc aattagaaga
    30721 tggacaacgt gacgaaagtg aagatcgtaa agaagaaaaa gatattgctg aggataaaaa
    30781 agaagctgat aaatctgatg aaaaagatga accagcacat cgacaagccc caaatatcga
    30841 aaaaccttac gatccaaatc acccagacaa taaataatcg attatattca gtacctcttt
    30901 ctatgataaa gttatagaaa gaggtacttt tatgatttaa ggaatacgag cctgagacat
    30961 aaaacaatgt tcaatgctct acaaagttat attggcagta gttgactgaa cgaaaaagcg
    31021 ctagtaacaa gcttttttca attctagtca ggcgccccaa cacagagaat ttcgaaaaga
    31081 aattctacag gcaatgcgag ttggggtggg acgacgaaat aaattttttg aaaatatcat
    31141 ttctgtccca ctcccattat ggcacgaata attaataatc aaacaagttg cttcattatt
    31201 tagaatgatg ggtgtagaat aagtacaatt gttgcatttt atgaagtaaa gtaattttta
    31261 aaatatagag taatagagga gattgaaata atgacacacg attatattgt taaagcatta
    31321 gcatttgatg gggagattag ggcttatgct gctttgacaa ctgaaactgt tcaagaagca
    31381 caaacgagac attatacatg gccgacagca tctgctgcaa tgggaagaac aatgacagca
    31441 acagctatga tgggcgcaat gttgaaaggt gatcaaaaat taacagtcac tgtagatggc
    31501 caaggaccta ttggacgaat tattgccgat gcaaatgcta aaggcgaggt gcgtgcttat
    31561 gtagaccatc cacaaactca ttttccatta aatgagcaag gtaaacttga tgtaagacga
    31621 gcagtaggga caaatggatc tattatggtt gttaaagacg ttggaatgaa agactatttc
    31681 tctggagcaa gtccaattgt ttcaggagaa cttggtgaag attttactta ttattatgct
    31741 acaagtgaac aaacaccttc atcggtaggt cttggtgtat tggtaaatcc tgataatacg
    31801 attaaagcag caggaggatt tatcattcaa gttatgccag gtgctaaaga tgaaacaatt
    31861 tcaaaattag aaaaagcaat tagtgaaatg acaccagttt ctaaattaat tgaacaagga
    31921 ttaacgccag aaggattact aaacgaaatc ttaggtgaag accatgtgca aattttagag
    31981 aaaatgcctg ttcaatttga atgtaattgt agtcatgaga aatttttaaa tgctattaaa
    32041 ggattgggcg aggctgagat tcaaaatatg atcaaagaag atcatggtgc tgaagcagta
    32101 tgtcatttct gtggaaataa atataaatat actgaagaag aattaaacgt gttgctagaa
    32161 agtttagcgt aatttaattt aaatcaatac gctaaaatgt ttatttttag cggtttagtg
    32221 aaatgtagaa ctaaatagtt gtataatcct tagtaatttt gtttgctttc tagaatttat
    32281 ttgataaaat aattctatat ccgataaata aactaagatt tcaacaacta actaaaaagg
    32341 agtgttctta atggcacaaa aaccagtaga taatattact caaattattg gcggtacacc
    32401 ggtagtcaaa ttgagaaatg tagtagatga caatgcagca gatgtttatg taaaattgga
    32461 atatcaaaat ccaggtggtt ctgtaaagga tagaattgct ttagcaatga ttgaaaaagc
    32521 agagcgagaa ggcaaaatta aacctggcga tacaattgta gaaccaacta gtggtaatac
    32581 aggtatcggt ttagcatttg tatgtgctgc taaaggatat aaagcagtat ttactatgcc
    32641 tgaaacaatg agccaagagc gtcgtaattt attaaaagca tacggtgcgg aattagtctt
    32701 aacgcctgga tcagaagcga tgaaaggtgc aattaaaaaa gctaaagaat taaaagaaga
    32761 acatggttac ttcgagccac aacaatttga aaaccctgcg aaccctgaag ttcatgagtt
    32821 aactacaggt cctgagttat tacaacaatt tgaagggaaa actatcgatg cgttcctagc
    32881 tggtgttggt actggcggta cgttatctgg tgtaggtaaa gttctgaaaa aagaatatcc
    32941 taacatcgaa attgttgcta tagagcctga ggcttctcca gtattgagcg gtggtgagcc
    33001 aggtccacat aaattacaag gtttaggtgc tggatttatt ccaggtactt tgaatacaga
    33061 aatctatgac agtattatta aagtaggaaa tgatacagcg atggaaatgt ctcgtcgagt
    33121 tgctaaagag gaaggtattt tagcaggtat ttcatcaggt gctgcgattt atgctgccat
    33181 tcaaaaagca aaagaattag gaaaaggtaa aacagtagta acagtattgc cgagtaatgg
    33241 tgaacgctac ttatcaacac ctttatattc tttcgatgac taattaatgt catttaaaag
    33301 agtgagctat ctttttgaga taacttgctc tttttttcta ccatgtataa ttttaaaaat
    33361 atgagcgtta aattaaacat ttttctgata aaaacatcca gtgaatgata agataataaa
    33421 cgtacatact aataactagt aaatcgcagg agtaaatttt attagagtta aacaatacat
    33481 aattaaaggg tggttaacat gactaaaaca aaaattatgg gcatattaaa cgtcacacct
    33541 gattcattct cagatggtgg aaaatttaat aatgttgaat cagcagtaac tagagtgaaa
    33601 gcgatgatgg atgaaggtgc tgacattata gatgttggag gtgtttcaac gagacccggc
    33661 catgaaatga ttactgtgga agaagagttg aatagagtat taccagttgt tgaagcgatt
    33721 gtcggttttg atgtaaaaat ttcggttgat acttttcgta gtgaagttgc tgaagcttgt
    33781 ttaaagctag gtgttgatat tattaatgat caatgggcag gcctttatga tcatcgcatg
    33841 tttcaagttg tagccaaata cgatgcggaa attgttttaa tgcataatgg caatggtaat
    33901 cgtgatgaac cggttgtcga agaaatgtta acatctttgt tagcacaagc acatcaagct
    33961 aaaatagctg gtataccttc aaataaaatt tggctagatc caggtatagg tttcgctaaa
    34021 actagaaatg aagaagccga agttatggca agactggatg aacttgttgc aacagaatat
    34081 ccagttttat tagcgacaag ccggaaacgt ttcactaaag agatgatggg ttatgataca
    34141 acaccggttg aaagagatga agtaactgca gctacgactg catatggtat tatgaaaggc
    34201 gttagagcag tacgcgttca taatgtcgag ttgaatgcta aattagctaa aggtatagat
    34261 tttttaaagg agaatgaaaa tgcaagacac aatttttctt aaaggtatgc gcttttatgg
    34321 atatcatggt gctttatcag ctgaaaatga aataggacaa attttcaaag tggatgtaac
    34381 tttgaaagta gacttatctg aagctggtcg tactgataat gttattgata cagttcatta
    34441 tggtgaagtg ttcgaagagg ttaaatcaat tatggaaggt aaggccgtta atttacttga
    34501 gcatctagct gaacgtattg caaatcgtat aaattcacaa tataatcgtg taatggaaac
    34561 gaaagtgaga atcactaaag aaaacccacc gattccgggt cattatgatg gagtaggtat
    34621 cgaaattgtg agggagaata aatgattcaa gcatacttag gattaggtag taatattggt
    34681 gatagagaaa gccagttaaa cgatgctata aagattttga atgaatatga tggtattaac
    34741 gtatctaata tttcttcgat ttatgaaaca gcaccagttg ggtatactga gcaacctaac
    34801 tttttaaatt tgtgtgttga gattcaaaca acactcacag tattacaact gttggaatgt
    34861 tgtttgaaga cagaagaatg tttacaccgt gttagaaagg aacgatgggg tcctagaact
    34921 ttagatgtgg atattttgtt gtatggagaa gaaatgatag atttaccaaa tctgtcggtg
    34981 ccacatccga gaatgaatga acgtgcattt gttttaatcc cattaaatga tatagcagca
    35041 aatgtcatag aaccacgttc gaaattgaaa gtgaaagatt tagtttttgt cgatgacagt
    35101 gtaaggcgat ataaataatg tattgttgag aacattcata tttattagga atagatgatt
    35161 gattattgga gatgtttcgg cattttgatt atctctaaat gcatttgatt tcgaattagt
    35221 atatgatgat ttagtatgtg ttagaattaa gttatttcat aaaatcaaag taagcaatat
    35281 ttgataaatt gctgtaattt atttggactt ttgttgaagt ttgtgaaaga taatttttat
    35341 acatttactt agggttgtat taatgataat cgtattttaa atgaataagt aattcataaa
    35401 cgaaaacaga aattgcttta ttacatttac aacctcttgt aaaattaaaa aagtgataaa
    35461 attgatgtta tgaatataag aaaacatgcc acatcataac tttaaggtgc aatggttaat
    35521 ggtaaagtat tagaaacatc gaaagagatg tatgttgagc aaaaatgtct gatattttat
    35581 aaaactttaa aggaaaatgt ttgagtgtac cagttggaat actaaaggat tacaacaagt
    35641 taaaggagag aaagttatgt cagaagaaat gaatgaccaa atgttggttc gacgtcaaaa
    35701 attacaagaa ttatatgatc ttggtataga cccgtttggt tctaaatttg accgttcagg
    35761 tttatctagt gatttgaaag aagagtggga ccagtattct aaagaagaat tggtagaaaa
    35821 agaagcggat agtcatgtcg ctatagctgg acgattaatg actaagcgtg gtaaaggtaa
    35881 agcaggattt gcacacgttc aggacttagc tggacaaatt caaatatacg ttcgtaaaga
    35941 tcaagttggc gatgacgaat ttgatttatg gaaaaatgct gatttaggcg atatcgttgg
    36001 tgttgaaggt gtaatgttca aaacaaatac tggcgaatta tcggttaaag cgaagaaatt
    36061 cacgctacta actaaatcat tgcgaccatt accggataaa ttccacggtt tacaggatat
    36121 tgaacagaga tatcgtcaaa gatatttaga tttaattacg aacgaagata gcactcgtac
    36181 atttattaat cgtagtaaaa tcattcaaga aatgcgtaat tatttaaata ataaaggttt
    36241 cttggaagta gaaacaccta tgatgcacca aattgctggt ggagcagctg ctagaccatt
    36301 tgtaacacat cataatgcat tagatgcaac gttatacatg cgtattgcta ttgagttgca
    36361 tttaaaacgt ttaattgtcg gtggacttga aaaagtatat gaaattggta gagtattccg
    36421 taatgaaggt gtatcaacta gacataaccc tgaattcaca atgattgaat tatatgaagc
    36481 atatgcagat tatcatgaca ttatggattt aacagaatct atggtgagac atattgccaa
    36541 tgaagtatta ggttctgcaa aagtacaata caatggggaa acgattgatt tagaatctgc
    36601 ttggactcgt ttgcatattg ttgatgctgt aaaagaagct actggtgtag atttttatga
    36661 agttaaaagt gatgaagaag ctaaagcttt agctaaggaa catggtattg aaattaaaga
    36721 tacaatgaaa tatggtcata ttttaaatga attctttgag caaaaagttg aagaaacact
    36781 tattcagcca acgtttatct atggtcatcc gactgaaatt tcacctttag cgaagaaaaa
    36841 acctgaagat cctagattta ctgatcgttt cgaattgttc attgtaggta gagagcatgc
    36901 aaatgcattt actgaattaa atgatcctat tgatcaaaaa ggtcgttttg aagcgcaact
    36961 tgttgaaaaa gcgcaaggta atgatgaagc gcatgaaatg gatgaagatt acattgaagc
    37021 gttagaatat ggtatgcctc cgacaggtgg tcttggtatc ggtattgaca gattagttat
    37081 gttattaact gactctccat caatcagaga cgtattatta ttcccttata tgagacaaaa
    37141 ataaatgacg ttgattgtta gtaagagctc tcgtgtatac aacatgtgta tgcgagggtt
    37201 ttcttaatta tggtaattag ttcgtgtttg aatgtttttg atagtaatag ttaacgataa
    37261 tggtgctatt tttgactgtt aaacaaggta gttagctgtt agatgaaaat gacgatacgt
    37321 atatagatta gttctgatga gaaatgttaa tgatagacat tgagatatct tataacaaaa
    37381 atttctatat tagtataatt tatcttaatc agctataaaa gtactttaaa attgtataga
    37441 atgtgtatgg ttttgtacat atgtgtatga tagaatacta aaagtgttat gaattagaga
    37501 gcacgagaaa tgtcagtttt gaagaataaa aaagttgatt aaaagtgttg actttatcaa
    37561 ttgaatgaag taatatataa aagtcgtcaa aaacagacga aacacactaa aaactgatgt
    37621 gacaaagttc acaacaaagt gtaaaattaa ctattgcgcc ttattaatta agcgtgtatc
    37681 ataaataagt aagttatttt gtctggtggc tatagcaagg aggtcacacc tgttcccatg
    37741 ccgaacacag aagttaagct ccttagcgtc gatggtagtc gaacttacgt tccgctagag
    37801 tagaacgttg ccaggcaaat gacaaatcgg agaattagct cagctgggag agcatctgcc
    37861 ttacaagcag agggtcggcg gttcgaaccc gtcattctcc accatttatt cttacatata
    37921 gccggcctag ctcaattggt agagcaactg acttgtaatc agtaggttgg gggttcaagt
    37981 cctctggccg gcaccatctt ttgagccatt agctcagctg gtagagcatc tgacttttaa
    38041 tcagagggtc agaggttcga atcctctatg gctcattacg atttaatttt tatatttagc
    38101 aaaataatgc agaagtagtt cagcggtaga atacaacctt gccaaggttg gggtcgcggg
    38161 ttcgaatccc gtcttctgct ccattatttt gccggggtgg cggaactggc agacgcacag
    38221 gacttaaaat cctgcggtga gagatcaccg taccggttcg attccggtcc tcggcaccat
    38281 tttagcgccc gtagctcaat tggatagagc gtttgactac ggatcaagag gttatgggtt
    38341 cgactcctat cgggcgcgcc atttttaaat taattgaata acgggaagta gctcagcttg
    38401 gtagagcact tggtttggga ccaaggggtc gcaggttcga atcctgtctt cccgattact
    38461 tctcaaattc cattttatgg gggcttagct cagctgggag agcgcctgct ttgcacgcag
    38521 gaggtcagcg gttcgatccc gctagtctcc accatttatt tttacacgat gaacattgaa
    38581 aactgaatga caatatgtca acgttaattc caaaaaacgt aactat
//
LOCUS       DAKSQE010000013        36967 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.013, whole genome shotgun sequence.
ACCESSION   DAKSQE010000013 DAKSQE010000000
VERSION     DAKSQE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 36967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36967
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.013"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            56..1411
                     /gene="brnQ"
                     /locus_tag="O0V33_000536"
     CDS             56..1411
                     /gene="brnQ"
                     /locus_tag="O0V33_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCX8975209.1"
                     /translation="MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIA
                     NLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPR
                     LATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
                     LLAIILLFAFIHPLGGISDAPISKQYQSNALFNGFLDGYNTLDALASLAFGIIIVATI
                     KKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQIT
                     QYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLF
                     ANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKIIYQCTIFFTMIAALVDG
                     LKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIPQ
                     A"
     gene            1664..2848
                     /gene="rocD"
                     /locus_tag="O0V33_000537"
     CDS             1664..2848
                     /gene="rocD"
                     /locus_tag="O0V33_000537"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCX8975210.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVITEGKGGKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNS
                     GTEAVEAAIKIARKWGSDVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKSGFHP
                     LLQGTKTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQI
                     LLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVEQLIKRGILCKDTHRTIIRLSPPLVIDKEEINQIVAAFQDVF
                     KN"
     gene            2887..3918
                     /locus_tag="O0V33_000538"
     CDS             2887..3918
                     /locus_tag="O0V33_000538"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCX8975211.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSLDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMARGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLKQSPVYP"
     gene            3930..5171
                     /gene="argJ"
                     /locus_tag="O0V33_000539"
     CDS             3930..5171
                     /gene="argJ"
                     /locus_tag="O0V33_000539"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HCX8975212.1"
                     /translation="MKHQETTSQQHNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASSAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DAQQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHINDANFATPGAFNEA
                     ILTTDTCTKHIAVSLNIDGKTITIGGSAKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANQQVEHQILNQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            5187..5957
                     /gene="argB"
                     /locus_tag="O0V33_000540"
     CDS             5187..5957
                     /gene="argB"
                     /locus_tag="O0V33_000540"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCX8975213.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQQQCSAIGLCGLD
                     AQLFEIKRFDQQYGYVGVPTTLNIDSLSYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIAASLEAPIYVLSNIAGVLINDVVIPQLPLADINQYIEHGDIYGGMIPKVLDAKN
                     AIKNGCPKVIIASGNKPNIIEAIYNNDFVGTTILKSSV"
     gene            6242..6721
                     /locus_tag="O0V33_000541"
     CDS             6242..6721
                     /locus_tag="O0V33_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HCX8975214.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMQLPWVSFIVHFSFSIVIAIIYCILVKKYAYLAMGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPFQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(7050..7694)
                     /locus_tag="O0V33_000542"
     CDS             complement(7050..7694)
                     /locus_tag="O0V33_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCX8975215.1"
                     /translation="MKVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGQAPWGYKKVSVFQLLSS"
     gene            complement(7707..14882)
                     /locus_tag="O0V33_000543"
     CDS             complement(7707..14882)
                     /locus_tag="O0V33_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HCX8975216.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNIEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKFKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHLNRNDDKDNQDASHIALDSNYFRLENNSDIHIDSYLPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKSVLQNMSQLQCAKSSLSLETIFHCYHQMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITKENGNKRTTVDELNLMTE
                     RDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNYVAVQFDGVFITYQTLNARANDL
                     AQRLRNQYGVEPNDRVAVIAEKSIEMIVAMIGVLKAGGAYVPIDPNYPSDRQEYILKD
                     ATPKVVITYQVVYENGKRDINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHQNHYVPLNEKTTILLSGTIAFDAATFEIYGALLNGGKLIVAKT
                     EQLLNPIALEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQRPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPEAIVKDDYVLSSAQKRMYLLWKSNHKDTVYNIPFLWRLSSE
                     LNVAQLRQAVQHLIARHEILRTQYIVVDDEVRQRIVADVVADFKEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSLDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDCDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQIRDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFDEVVSRQGNHVALVMNDLTMTYETLHNYVDAIAHMLLSNGVGKGQPVALIT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPKKRQGAILEDAKVTAVMSYGVEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGDQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPEQLQ
                     QLINKHRVTVASIPLQMCSVMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHQCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENAILAIRGISDCVVTVSHFDTHDILNAYYVGEQQMEQDLKQYLNDHLPKYMIPKT
                     ITYIDCIPLTTNDKVDTTRLPNPSPIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMHQNQQSLVALP
                     DNLSELQKIVMSRYNLGILEDSLSHRPLGKTLLTGATGFLGAYLIEALQGYSHRIYCF
                     IRADNEEIAWYKLMTNLNDYFSEETVEMMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVKNGLDGRIVRVGNLTSPYNGRWHMRNIKTN
                     RFSMVMNDLLQLDCIGVSLAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKCKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTKLTLKIMD
                     HISEKWPTITNNWLYHWAQYIKTIFNK"
     gene            complement(15329..16579)
                     /locus_tag="O0V33_000544"
     CDS             complement(15329..16579)
                     /locus_tag="O0V33_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCX8975217.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGIFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKKHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKIVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGAFLTTLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNTN"
     gene            complement(16965..17990)
                     /locus_tag="O0V33_000545"
     CDS             complement(16965..17990)
                     /locus_tag="O0V33_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HCX8975218.1"
                     /translation="MKIVALFPEAVEGQDNQLLNTKKALGLKTFLEERGQEFIILADN
                     GEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSTSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            complement(18164..18523)
                     /locus_tag="O0V33_000546"
     CDS             complement(18164..18523)
                     /locus_tag="O0V33_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCX8975219.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESVVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
     gene            complement(18737..19768)
                     /locus_tag="O0V33_000547"
     CDS             complement(18737..19768)
                     /locus_tag="O0V33_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCX8975220.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEATLR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVAVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNAQNGINQYLEYDVEGFKKRYRQLREEYYAILDDGNLTS
                     HLNELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLEAELKG"
     gene            complement(19781..20542)
                     /locus_tag="O0V33_000548"
     CDS             complement(19781..20542)
                     /locus_tag="O0V33_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8975221.1"
                     /translation="MTRLTINKIILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWTFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            complement(20539..21513)
                     /locus_tag="O0V33_000549"
     CDS             complement(20539..21513)
                     /locus_tag="O0V33_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCX8975222.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRASKEPSNNAQHQQVIKIGYLPI
                     THSANLMMTKKLLSQHNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEALREQL
                     KIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDA
                     YCCVLVLRGELLDQHKAVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSA
                     AWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            complement(21527..22267)
                     /locus_tag="O0V33_000550"
     CDS             complement(21527..22267)
                     /locus_tag="O0V33_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8975223.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NIIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGFQQKISDE
                     EINAQLKLVDLEGRGKHFPEQLSGGMKQRVALCRAHVHKPNVILMDEPLGALDAFTRY
                     KLQDQLVQLKHKTESTIILVAHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKIRNEIMETFALNHHQVEPEYYL"
     gene            complement(22609..23121)
                     /locus_tag="O0V33_000551"
     CDS             complement(22609..23121)
                     /locus_tag="O0V33_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HCX8975224.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKASSLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEAGISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
     gene            23301..23504
                     /locus_tag="O0V33_000552"
     CDS             23301..23504
                     /locus_tag="O0V33_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975225.1"
                     /translation="MDKESVVASLARNKKIAVETMTGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVDTIKDIDENHLDDAT"
     gene            complement(23566..24525)
                     /locus_tag="O0V33_000553"
     CDS             complement(23566..24525)
                     /locus_tag="O0V33_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCX8975226.1"
                     /translation="MNGKKANTINRYKYFHHVDHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTVILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDSIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIIADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(25172..26659)
                     /locus_tag="O0V33_000554"
     CDS             complement(25172..26659)
                     /locus_tag="O0V33_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCX8975227.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVKVAQEAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPNGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGDPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            27086..27469
                     /locus_tag="O0V33_000555"
     CDS             27086..27469
                     /locus_tag="O0V33_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HCX8975228.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRIGLII
                     MVIIQTAVLFTFVKTLPKSNNKIEE"
     gene            27476..27802
                     /gene="isdI"
                     /locus_tag="O0V33_000556"
     CDS             27476..27802
                     /gene="isdI"
                     /locus_tag="O0V33_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HCX8975229.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TKDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(27867..29012)
                     /gene="cap8P"
                     /locus_tag="O0V33_000557"
     CDS             complement(27867..29012)
                     /gene="cap8P"
                     /locus_tag="O0V33_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HCX8975230.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLAGITANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKESIFITGNTVIDALSTTVQNEFVSTIINKHKGKKVILLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDALEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRSDKPDEFVPLRRK"
     gene            complement(29089..30351)
                     /gene="cap8O"
                     /locus_tag="O0V33_000558"
     CDS             complement(29089..30351)
                     /gene="cap8O"
                     /locus_tag="O0V33_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HCX8975231.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEVYEEVLSSGKLKVSTTPEASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILPFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
                     ELVHNNRIIGGVTKACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPSYVVDTTKQIIKALSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            complement(30405..31292)
                     /gene="capN"
                     /locus_tag="O0V33_000559"
     CDS             complement(30405..31292)
                     /gene="capN"
                     /locus_tag="O0V33_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HCX8975232.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KGYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYG
                     KEGQVGKSDQIDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTLLINMPSMLNKYFNKLSVFRKLFGNLTYSNTLYENNNALEVIPG
                     KMSLVIADIMDETTTKDKA"
     gene            complement(31292..31849)
                     /gene="cap8M"
                     /locus_tag="O0V33_000560"
     CDS             complement(31292..31849)
                     /gene="cap8M"
                     /locus_tag="O0V33_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HCX8975233.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTAYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKANVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            complement(31860..33065)
                     /gene="cap8L"
                     /locus_tag="O0V33_000561"
     CDS             complement(31860..33065)
                     /gene="cap8L"
                     /locus_tag="O0V33_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HCX8975234.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGIRIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYI
                     NKHVYKNAENVIVLGTEMKNYLLNHQISKNPDNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQPQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKPLVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            complement(33099..34337)
                     /gene="cap8K"
                     /locus_tag="O0V33_000562"
     CDS             complement(33099..34337)
                     /gene="cap8K"
                     /locus_tag="O0V33_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8K"
                     /protein_id="HCX8975235.1"
                     /translation="MRLNKFIGDSFLMILSSGIAQVILIITTPIITRLYSPTEFGEFT
                     IFSNIAMILIPIINARYDLLIVNTKNDRSANILSQISFLISLLILLILIPIFAISAWL
                     YPNFILDFIFIIIMLFLVSLTNIFTNYLNKERKYKVLSLINVFRAGSMALLQIIFGLL
                     ALGSLGLIIGFSLSYIAGITLGYKTFKKHFNIVRDKEETKALFLENKNQLVYSTPSIL
                     LNSLSFSVVVFFIGILYTNTEVGIYGMAIRVLGIPVTIISLGLSKIFMQQANDYYIEY
                     GNFRNLLLKFSSILVIVSIILYVPLYLFSEELVNILLGHSWVDAITVIKIVIPLFVIR
                     LIVSTVSLSVIVLQKQQLELILQALFLIGTTATFVISKMLNLTFLNFVSINTVVLIVS
                     YMIFFIALYYFAKNKQFKNS"
     gene            complement(34346..34903)
                     /gene="cap8J"
                     /locus_tag="O0V33_000563"
     CDS             complement(34346..34903)
                     /gene="cap8J"
                     /locus_tag="O0V33_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8J"
                     /protein_id="HCX8975236.1"
                     /translation="MIVKTFMKSKIFRLMNTPLLLFYKKEYLTGYYFENKVAGWLWAW
                     KAVPFKLLGINTSLPFPADITVRMHNPNNIVFDKNDIHIFQSPGTYFNNFSAVIYIGR
                     GVYIAPNVGIITANHNIKNLKSHAPGEDVKIGNYSWIGMNSVILPGVELGEHTIVGAG
                     SVVTKSFPEGNVVIGGNPAKVIKKI"
     gene            complement(34900..36294)
                     /gene="cap8I"
                     /locus_tag="O0V33_000564"
     CDS             complement(34900..36294)
                     /gene="cap8I"
                     /locus_tag="O0V33_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8I"
                     /protein_id="HCX8975237.1"
                     /translation="MNKIYNVTSYVIAILMFPCLMLGDKPLLFLAPISYGVGKLFISF
                     SNNPNFKFSKIVYDVLGFLRLVFIPAMIVFFQDSTIDNLPLGQDYFNQAVIYMSVEFI
                     IGSLFILILSKLFKHEVVSRNSFTLSGSSIYYIVFGLVICGIFVAFPEVRKNISFLII
                     KTDAMGRGTEATSGLNVLFVMLFQLALALLFLIIAYASYKKYKENPKIIYVILPLAIG
                     ILNISLIVGERRSYQLYTMVAVLTVVSLLFSKHKRRINIIIISVGIFVLALMTLYKEL
                     YVFNYSSYSEALNSTSVSNLKIVDTLQSYFYGPSNIAASIDYLNYYNGSFKQYLFDNT
                     RAVFGFNFFVDKKQLITSQLFNQLIYGSKQLTGHLISSAGYGIIYFGPLFFYLNLIAN
                     IFFAFLSEYIIRKSHSLEVIFIGTYIYMRLITSIFSHPTPLITLISMILVVYVIAIIP
                     GIIIKKFTKKVGIE"
     gene            complement(36287..>36967)
                     /gene="cap8H"
                     /locus_tag="O0V33_000565"
     CDS             complement(36287..>36967)
                     /gene="cap8H"
                     /locus_tag="O0V33_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8H"
                     /protein_id="HCX8975238.1"
                     /translation="KIVDASRLTTLSSEGFLKFLPKSEKYRIIYSYNMSIIRESNVTD
                     EFKKRWPINIGFIGNVRFNEINQKLIKELANDSRFHMQYFGTGSEKLEVFARENFINN
                     ITFSGGFDLEETPKYLNEIDILNNLFGNKNIALDTALSIRMYYALFLNKPIITTDDTF
                     TATEANKFGLGFSVNPENLKGIGDELMDWYNNLNVMDINHKREAYRNDVIENNKQFYQ
                     EIGRIFNE"
BASE COUNT        12070 a         6919 c         5468 g        12510 t
ORIGIN      
        1 attatcttaa ttaagtagat ttttataagt tctaaaaagg agaacaaata catatatgaa
       61 gaagaaacta acatttaaag aaaacatgtt tattggttct atgttatttg gtttattctt
      121 tggtgccggc aaccttatct tcccaataca cttgggtcaa gctgctggtt ctaacgtttt
      181 tatcgctaac ttaggatttt taattacagc aattggctta ccatttctag gtatcattgc
      241 tattggtatt tcaaagacat ctggtttatt tgaaattgca tcgcgtgtta ataaaacata
      301 tgcttatatt ttcacgattg ccttatatct agttatcgga ccatttttcg ccttacctag
      361 actggcaact acatcatttg aaattgcatt ttcgccattt ttatcaccaa agcaaatcac
      421 tttatattta tttattttca gcttcgtctt ctttgtgatt gcatggtttt ttgcgagaaa
      481 gccatcaaga attttagaat atatcggtaa atttttaaat ccggtattct tagtattatt
      541 agcaattatt ttattatttg cttttatcca tccattaggt ggcatatctg atgcacctat
      601 tagtaaacaa tatcaatcaa acgccttatt taacggcttt ttagatggat acaatacctt
      661 agatgcgcta gcgtcattgg catttggtat catcattgtt gcaacgatta aaaagttagg
      721 tatcgaaaat ccaactgata tcgctaaaga aacaattaag tctggtacta tcagtatcat
      781 tatgatgggg atcatttata ccctactagc aatcatgggt acattaagta ttggtcattt
      841 caaacttagt gaaaatggtg gtattgcctt agcgcaaatt acgcaatact acttaggtaa
      901 ctacggtatc gtcttgttat cactcatcgt tatggttgct tgtttaaaaa cagctatcgg
      961 tttaattacg gcattttcag aaacatttga acaccttttc cctaaaatga attacctagc
     1021 gattgcaaca gttgtgagct ttatctcatt cttattcgcg aatgttggct taactaaaat
     1081 tattatgtac tcagtcccag tgttaatgtt cttatatcca ttagcaattg ccctgattgt
     1141 actaacatta tttagcagca aattccatca ttcaaaaatt atttatcaat gtaccatttt
     1201 ctttacaatg attgctgcat tagtagatgg attaaaagct agtccagagt tcatttcaag
     1261 cacatcattc tcacaaactt tgattaattt cagccaaaaa tatttaccat tatcagacat
     1321 tggtatgggc tgggttgttc tcagtttgat tggtttcatt atcggcttca ttatttataa
     1381 aattaagcat cgtaaaattc cacaagcata atactatgcc acagtcatat gttaaacata
     1441 tgcttgtggc attttttatt catactacat taaactgcac tcgtatacat acatattaat
     1501 gattatccac gaaaaatatt agtactttca ttttacgaat cacattaatg catacacaac
     1561 tttatcttta tattattaaa tttacatttg acacttatat tgaacaactg taatatatta
     1621 atattaattc tttaaattgt ataaatataa aggagggcga ccgatgaatt caatcattga
     1681 attaactgat tattatagct ctaataatta tgcaccactg aagcttgtaa ttactgaagg
     1741 gaaagggggc aaagtttggg atactgatgg caaacaatat atagattgca tttcgggttt
     1801 ttcagttgca aaccaaggcc attgtcatcc aacaattgtt aaagcgatga cagaacaagc
     1861 ttcaaagttg tctatcattt cacgtgtcct ttatagtgac aatctcggga aatgggaaga
     1921 aaaaatttgt catcttgcta agaaagacaa agtactcccc cttaactctg gtactgaagc
     1981 tgttgaagca gccattaaaa ttgctagaaa atggggctct gacgttaaag gcatcactga
     2041 cggacaagtt gaaatcatcg ctatgaataa caattttcac ggtcgtacac ttggctcatt
     2101 atcactatct aaccacgacg catataaatc aggatttcac cccctacttc aaggtactaa
     2161 aacagtagat tttggagata ttgagcaatt aacacaagct atttcaccaa atacagcagc
     2221 aattatttta gaaccaattc aaggtgaagg tggcgttaat ataccaccga aaggatatat
     2281 ccaagctgtg cgtcaactat gtgataaaca tcaaatatta ttgattgcag atgaaattca
     2341 agttggtctt ggtagaactg ggaaatggtt tgcgatggaa tgggagcaag tcgttccaga
     2401 catttatatt ttaggtaagg cattgggtgg cggcttatac cctgtatctg ctgtgcttgc
     2461 aaataatgat gtcatgcgag ttctaacacc aggtacacat ggctcaacat ttggtggtaa
     2521 ccctttagcc attgcaatat cgacggcagc gcttgatgta cttaaagatg aacaactggt
     2581 tgaacgatca gaacgcttag gttcattttt attaaaagcg ttgctacaac ttaaacatcc
     2641 tagtattaaa gaaattagag gtcgtggttt atttattggc atcgaactta acacagatgc
     2701 cgcacctttc gttgaacaac tgattaaacg tggcatttta tgcaaagaca cgcatcgtac
     2761 tatcattcga ttgtcaccac ctctagtcat tgataaagag gaaatcaatc aaattgttgc
     2821 agcttttcaa gacgttttta aaaattaaca attaatcatt tatatatgat ataggaggga
     2881 ttcatgatga ttaaagtagg tatcgttggc ggtagcggtt atggcgcaat tgaattaatt
     2941 cgattattac aaacacatcc tcatgtaacg attgcacaca tctactcaca ttcaaaagta
     3001 gatgagccgt tgaaattaac atttccacat ttacaacata ttatgcaaca tttcgaagca
     3061 cttacagtgg acaataatga ctgtgatgta attttctttg ctacaccagc acccgtgagt
     3121 aaaacatgta tccctccttt agtagaaaaa ggaattcatg ttattgattt atctggtgca
     3181 ttcagaatta aaaaccgtga aatatatgaa gcatattaca aagaaactgc tgcagcacaa
     3241 gatgatttga atcatgctat ttacagcatt tcagaatggc aatctttaga taacaatgga
     3301 acgaaactca tttctaaccc tggctgtttc cctacagcaa cgttattagc attacatcca
     3361 cttattagcg aaaaaattgt agatttgtca tctattatta ttgatgctaa gaccggcgtg
     3421 tcaggtgctg gtcgttcatt atcacaacgt gttcattttt cagaaatgaa tgaaaatcta
     3481 agcgcttatg caatcggaaa ccataaacac aaaccggaaa tcgagcaata tttatctatc
     3541 attgcgggtc aagatgtatc agtcatattt acaccacatc tcgtaccaat ggcacgaggt
     3601 attttatcaa caatatatgt caaattatca tctgaatata cgactgaatc attacataaa
     3661 ttaatgacct cttattatgc taatcagcca tttgtcagaa ttagagatat tgggactttt
     3721 ccaaccacaa aagaagtact cggtagtaac tactgcgata tcggtatcta tgtagatgaa
     3781 acaacgcaaa cagcaatttt agtatcagtg attgataacc ttgtcaaagg ggcaagtggt
     3841 caagccattc aaaacttaaa tatattatat gattttgaag ttacgactgg cctaaaacaa
     3901 tcaccagttt atccataagg ggtgttagaa tgaaacatca agaaacgaca tcacaacaac
     3961 ataacttttc aattattaaa catggcgata tcagtacacc tcaaggcttc acggccggtg
     4021 gtatgcacat cggtttacgc gctaacaaaa aagactttgg ctggatttac tcatcgtctt
     4081 tagcaagttc agctgccgta tatactttaa accaatttaa agctgcacca cttattgtca
     4141 ctgaagacac tttacaaaag tctaaaggga aattacaagc acttgttgtt aattcagcta
     4201 atgcaaattc ttgcacaggt cagcaaggta tcgatgatgc acaacaaact caaacatggg
     4261 ttgctcaaca acttcaaata ccatctgagc atgttgctgt tgcttcaact ggtgttattg
     4321 gagaatattt gccaatggat aaaattaaaa ctggtacaga acatattaat gatgctaatt
     4381 ttgcaacgcc aggtgcgttt aacgaggcaa ttttaacaac tgatacatgt acaaagcata
     4441 tcgctgtatc actaaatatt gatggtaaaa ccattacaat tggtggcagt gctaaaggat
     4501 caggtatgat tcacccaaat atggctacca tgcttgcttt tataacaacc gatgcatcaa
     4561 ttgaatcgaa tacacttcat caattattaa aatcttcgac tgaccataca tttaatatga
     4621 ttactgttga tggcgataca agtacaaatg acatggtatt agtcatggca aatcaacaag
     4681 ttgaacacca aatacttaat caagaccatc cacaatggga aacatttgtt gatgccttca
     4741 attttgtctg tacattttta gctaaagcta tagccagaga tggcgaaggc gcaacaaagt
     4801 taatcagtgt caatgtttca ggcgctaaat caatatcaga cgcaagaaaa atcggtaaaa
     4861 ctatcgtaag ttcaaatcta gtaaaatcag ctatttttgg cgaagatgcc aattttggtc
     4921 gaatcattac agctattggc tatagcggat gtgaaattga tcctaactgc acatatgttc
     4981 aactgaacca aatacctgtc gtggataaag gtatggctgt actatttgat gagcaagcta
     5041 tgtcgaatac attaactcat gaaaatgtca caattgacgt tcagcttggt ttgggtaacg
     5101 ctgcagcgac tgcatacggt tgtgatttat cctatgatta tgtgcgtatt aacgcatcat
     5161 atcgaacata aggtggtgtt ggttagatga aatttattgt cattaaaatt ggtggcagta
     5221 cactaagtga catgcatcca tcaattatta acaacattaa gcatttacga tcaaacaaca
     5281 tctaccccat tatcgttcac ggcggtggcc catttattaa tgaagcatta tcaaaccagc
     5341 aaatcgagcc acactttgtt aatggcctaa gagtgactga taaagcaacc atgactatta
     5401 ctaaacacac gctcattgca gacgttaaca ctgcattagt agctcaattt aaccagcagc
     5461 aatgttctgc aataggctta tgtggtttgg atgctcaatt atttgaaatt aaacgttttg
     5521 atcaacaata tggatatgtc ggggttccaa ccactttaaa tatagactca ttgtcttatt
     5581 tatgtactaa atttgtacct atcatcaatt cgattggttt caataaccat gatggagaat
     5641 tttacaacat aaatgcagac acactcgcct attttattgc agcatcatta gaagcaccca
     5701 tttatgtact aagtaatatt gctggcgtgc taatcaacga tgttgttata cctcaactac
     5761 ccttagccga tattaatcag tatattgagc atggtgatat ttatggaggt atgattccca
     5821 aagtgcttga tgctaaaaat gctattaaaa atggctgtcc taaagttatc attgcatcag
     5881 gaaacaagcc aaatattatc gaagctattt acaataatga ttttgttggg acaacgatac
     5941 taaaatcatc agtatgaatt tgaatatgac cactcaatct tgtatttttc ggcttcgaaa
     6001 catagttgta cctaatttgt ttaatgatta tgcagcgtca ccttttcacc tgtctcattt
     6061 cacaaaagaa ttcttagaat acagtatgat acaatatgag atgaaaaaca ctataagtga
     6121 ttttaaaatt tgtaactcga aatattttat attttaaaag tttttcattg tgattatttt
     6181 cacaaaatac tataatgagg atagtaaata gagaggagtc cttaagttga caaaacgaca
     6241 aatgggtata ttcatttatg ctggaattat cggtggcttg ttatctggaa ttgtaaaatt
     6301 aggttgggag gtcatgtttc cacctcgcac accagaacgt aatgcaacga acccacctca
     6361 agagttattg caacaattag gatttagtag tgagtttacg catcaaacat atacattttc
     6421 aaatatgcaa ttgccttggg taagctttat tgtccacttt agtttttcta tcgtcattgc
     6481 aattatttac tgcatattag ttaaaaaata cgcttactta gcaatgggac aaggtgctgt
     6541 ttttggtatt gctatttggg tattattcca ccttatcatt atgccaatca tgcatactgt
     6601 acctgctgtg tgggatcaac cattccaaga gcatctgtca gaattctttg gccatatcgt
     6661 ctggatgatg acaattgaat tagtgcgaca acattttgtc tatcgctata aattaaatta
     6721 atacactgac taacattaac gtgagtttta aatcatcgtt tgagtatgat gattgatgct
     6781 cacgttattt tattaactga catgatataa ttctagccaa cttacatgag cattaaagtc
     6841 tcaaatgcgt cgtaacaagc cattattttt ggcaatttca atattgctca gtatattttc
     6901 accttatcac ttactttaat ctcgtcatga ttttgaatga tgccatcgtg tattcacctt
     6961 tcatttttcc aataaaaaaa catctaacag taaacattta ggcagtatag tttaaaatca
     7021 ctgccaaata aaactgttag acgccatgtt taactactca ataactgaaa tacagacact
     7081 tttttataac cccagggtgc ctgtcctaag aaacatacct gtaccataaa ctgatcaaaa
     7141 ataaattgtt tgaacttcac ttcacgtgat tgataaaagt gtgattgcgt catatcataa
     7201 atgtccaatc ctttgattaa accttcacca atcaattttg taaaactttc tttttgtgtc
     7261 catatttgat aaaaatcatt taaactacat atttgatgtg cttcgtttgt agagaaacac
     7321 gtcactaatg tacgccagtc taaacgttgt gatatctttt cgatatcaat accaactggt
     7381 tctttatcga caacacacac gatataagga taactatatg ataagctcac atagatgggc
     7441 tgtccatcac gattgtgttg aactatatct gccttacctc gtggcgaaat gtgataatgc
     7501 cattcatgtg gtaataaccc tgtgtcatgt tgaattccat attgcactaa aatatctccc
     7561 aatctgtgca tgagtttatc ttgattgtat ctatagttga ctgtacgcgg ttttttatat
     7621 gaccaacgac tttgtgatat taattcttca atacttttca agttactctg taattgcatt
     7681 acaaatactt tcataacttt ccctacttac ttattgaata ttgttttgat atattgtgcc
     7741 caatgataca gccaattgtt agttatcgtt ggccattttt cactgatgtg atccattatt
     7801 tttaatgtta attttgtatc tatcattgct agttgttgtt cacggtcaac actagttaat
     7861 ccaatcgttt cgtacatgtc ttgtttctgt aaaatttcat taaatgattc atcgctgacg
     7921 agttcaattt ctttgcactt aacacattct aataaagatt tcaccggcat tttattaggt
     7981 gataacacat ggtaaatgat ttgtggcgtg ttgacctgtg ctaatgcgac aatttgtctt
     8041 gcagtcgtat ccacaaaaga aaaatctaca ggcatttcag cgaggctaac cccgatacaa
     8101 tccagttgta acaaatcatt cattaccatt gaaaaacggt tagtctttat atttctcata
     8161 tgccatcttc cattgtatgg actcgtcaaa ttaccaacac gtacaattcg accatctaag
     8221 ccatttttta cagcttctaa tacttttaat tcactataaa atttactccg tgtatatggt
     8281 gatgttagta gttgtccttt atagacatcc gcttctgaaa atgtcacatc ttctgtatct
     8341 atatcaaaat aagttcccac acttatcgta gacacatata ttaaccttgc atgatgttgt
     8401 tgtgccaaac gtatgacatc aacagtacct tgaacattta ctttttcaaa ttcatcatca
     8461 tcaccaaagt gatctgtacg agcacctgca tgaataattg tatccatgtt ttctggtaaa
     8521 acaacatcat ccatacactc gaaatcacca acaatgactt caatgtttga taacatcatt
     8581 tcaaccgtct cttctgaaaa ataatcattt aaattcgtca tcaacttata ccatgctatt
     8641 tcctcattat cagcacgtat gaaacaataa atgcgatgac tgtatccttg tagtgcttca
     8701 atcagataag cacctaaaaa ccctgtagcg ccagtcaata gtgtttttcc tagaggtcga
     8761 tgacttagac tatcctctaa aatacccaaa ttataacgag acataacaat cttttgtaat
     8821 tccgaaagat tatccggtaa tgcaactaat gattgttgat tttggtgcat ataattaaca
     8881 atctgtcgca cggttttata ttgatataat gtctgcattg aaatatgatg gccaaatcgt
     8941 tttaaatgcg agacaactaa catcgcctct aatgagttac caccaagttc aaagaaatca
     9001 tcgtcaacac cgacatcatt ttgtttcaat acctctccga atacatcaac aaatgtctgc
     9061 tcaatttcat tagatggttc gctatatact ttattagact gttgtatagg tgatggattt
     9121 ggcaaacgcg tagtatccac cttgtcattc gtggttaatg gtatacaatc gatatacgtt
     9181 atagtcttag gaatcatata cttaggcaga tgatcattta aatattgctt caaatcctgc
     9241 tccatttgtt gttctccgac ataataagca ttcaatatat catgcgtatc aaagtgacta
     9301 actgtgacaa cacaatcaga tataccacga atagctaata ttgcattttc aatttcatca
     9361 agttcaatac ggtacccgtt aactttcact tgtttatcta ttcttcccaa aaattcaatt
     9421 tgaccatcag atgtataacg tgctaaatca ccactatgat acaactttcc tttaccaaat
     9481 ggattatttt gccatttatc agccattaat tccggacgat taatatatcc tatcgctaaa
     9541 ctatcacctg caatacacaa ctcgcctggc ataccaatac cgcataacaa accatctgac
     9601 ataatataca cttggatgtt agataagggt ttgccaattg gaatcgtctc aggtatcaaa
     9661 tcaccacatt gatgtgacca atacgatgtg atgactgttg actcagatgg tccataggca
     9721 ttgaaatacg tgccacaatg cttctcaata tatttaacaa aggatgccgt actagttgcc
     9781 ccgcctgtaa tcaatttttc aatataaaag tcttccataa cactacacat ctgtaatgga
     9841 atcgacgcca ccgtcacacg atgcttatta atgagttgtt gtaactgttc tggattaaca
     9901 cgttcctctc tatctggaat cacaagcgta tgaccattta acaaacaaca ataaatctcc
     9961 ataactgatg catcaaaaac aatatttgca tgttgcaaaa atacttcatt gtcgcctaat
    10021 tgcaattcag ttgaccatgc atgcactaaa ttcaacaaat ttcgttgtcg tatggcaacc
    10081 cctttaggca tcccggtcgt accagatgtg taaatagcat acatctcatt atctaacatc
    10141 gctgtgtttt cgagttgatc gccatgtaaa tcatcatatt gttcattttc ctttgattca
    10201 acaaaacctt tagcattttc caattgaatg actggtaatg tcgtttcaat ttcaacgccg
    10261 taagacatga ctgcagttac tttagcatcc tccaaaattg caccttgtcg ctttttcgga
    10321 aaatcaatat cgataggtat ataagatgca cctactttaa ctgtcgccaa catcgccgca
    10381 atcatttcaa aactacgttc tgttattaaa gcaactggtt gacccttgcc cacaccattt
    10441 gataggagca tgtgcgcaat agcatctaca tagttgtgta atgtttcata cgtcattgtc
    10501 aaatcattca tgactagcgc aacatgatta ccttgtcgtg agacaacttc atcaaagtaa
    10561 cttatgatag atttatttcc cgggacatta agcattcgat cgttaacatg cgtattgacc
    10621 caatttaaaa gttcctccgt gccgtttggt atatcacgaa tttgtagtgt atcttgatgc
    10681 ttcaaaatat aatcaatcat aatcatacat tgattaccca tgtgacgaac tgtttctgag
    10741 tgatataaat cggtattata ctcgatattg attgtatagt catcgcaatc ttcttcaatg
    10801 atgaaagata aatcaaattt cgccgtcacc gatttgggtt gaatgtgtgt taatttacta
    10861 tgcccaaaat gagcatgatt cgtttcattg ttttgtagta ctaacatgac atcaaataat
    10921 ggattccgtg aggcatcatg tgattgatct aagtcattta ctaaacattc gaatgggtat
    10981 tcttgatgct cgtatgcctc caaactcatt tccttaacct cttgtaaaaa ctgtgtccac
    11041 attttatcaa gtgatggttg ccctctatat accaacgtat tagcaaacat gcctagcatt
    11101 tgctccgtgc ctttatgcat acgcgcactc atgacactac cgacaacaac atcatctttt
    11161 cgagcatatc tgcttaacaa cgtcatgacc acactcataa agaacataaa atcagtaatt
    11221 tgatgctttt ctacatactt ttgaagtagc tgtctcattt gttgattcat tgtaaatgac
    11281 atcattgctc catttgtcgt tttaatattt ggtctaacat agtctgtcgg taagcttaaa
    11341 ataggtactt catctttgaa ttgagataac caatattgtc tatgtttcgt catatcacga
    11401 tgcgacatcc attcactata gtctttatat tgcaatttaa gtggtaacaa taatttatgt
    11461 tgatataatg cgttaagatc attcattaat tgtatattac tcataccatc attaataata
    11521 tgatgtgtat ctataaacag gtatgcatgt aatggacttc gaatgtatct cactctaatt
    11581 tggcttggct tttccaaatt aaaaggtgcc acaaattggc gcatgatttc ttgttcatcc
    11641 gtaaaatgcg tgttaacttc tttaaagtca gctacaacat ctgccacaat acgttgtcga
    11701 acctcatcat ctacaacaat atattgtgtt cgtagaatct catgtcgcgc tatcaaatgc
    11761 tgaactgctt gtcgcaattg agctacatta agttctgatg ataaccgcca taaaaaaggt
    11821 atgttataca ccgtatcttt atggtttgat ttccataata aatacattcg cttttgtgca
    11881 gagctcaaca cataatcatc tttaactata gcttcaggaa tcacttcata gttttgttct
    11941 tgaaccttag caatcgcttg cgctagttca aatacagttg gcttttgtaa taaatcacca
    12001 atttgtaatc gtttcccagt agatgcctct atccgattca ccactaacgt tgcttttaag
    12061 gaatggccac ctaattcaaa gaaattatca tgaataccta cttgattcac atgtaaaata
    12121 tctgcaaaaa tttggcatag caagtgttcg gtatctgtac tcggtgctac ataggcatcc
    12181 gtatcgacat agtccatgat aggcaatgcc ttcttatcta atttcccatt aatagtaata
    12241 ggaatttgct caatatgcat aaaattaact ggtatcatgt actcaggtaa ggtcatacgt
    12301 aattgtgatt taatcttatt atgtgataat gtatgcatcg cttcataata agcaacgata
    12361 tactggtctt gatcatgatt ttgaacaata acaacagctt tattaatacc ttgtatacgc
    12421 tcgagcgcat gctcaacctc tgacaactca atcctaaatc ctcgaatctt aacttgtttg
    12481 tcctttcgat ataaataatc tatgttgcca tcgggtaaca aacgtacgat atcaccactt
    12541 ctatacatca gctgatttat atttgaatct ttgataaatt tatctgctgt caattctggc
    12601 tgatttaaat aacctgcagc caacccaaag ccacttgtac ataattctcc aggaatacca
    12661 acgccacacc gacgctcgcc ttgcatgata taaacatgag taccaagaat cggtttacca
    12721 ataggaatac gatttggaac tttgttaggt atattatacg tcgttgtaaa tgttgtattt
    12781 tcagttggtc cataaccatt aataatttga ggatgcttcg gtctttgatt aagcaaatcc
    12841 acccacttag cattcaatac ttctccacca attaataaat actttaacgg taccaatact
    12901 tctattcgtt cactagcaat ctgattaaat aatgaggagg ttaaccacat agtattgacg
    12961 tcattttcat tgattaattg ttctaacgct attggattta ataattgttc tgttttagca
    13021 acaatcagtt ttccaccatt tagcaatgca ccatatattt caaatgttgc agcgtcaaag
    13081 gctatagttc ctgataacaa aatcgtcgtc ttttcattta atggtacata atgattttga
    13141 tggaccaagc gaacaatacc tcggtgcgga attagtgtcc ctttagggtt accagttgtc
    13201 cccgacgtgt aaataacata tgcatgatct tctaacgtgt tacatttaga aagattatca
    13261 atatttttcc acgctatctt gttcaaatca atgtgattaa tatctcgttt accattttca
    13321 tatactactt gatatgtgat aacaacttta ggcgttgcat cttttaaaat gtactcctga
    13381 cgatcacttg gatagttcgg atcaattggc acgtaagccc caccagcttt caacacacct
    13441 atcatcgcta ctatcatctc aatacttttt tcagctatga cagcgacacg atcattaggt
    13501 tcaacaccat actggtttct caaacgttgt gctaaatcat tcgcgcgtgc attcaatgtt
    13561 tgatatgtta taaacactcc gtcaaattgc acagcgacat aattcggtgt tgcttcaact
    13621 tgttgctcaa ataaggtaac aactgtttgc gcatcatcta tctcaggtaa acttaaattg
    13681 atatcgtcat ataattgaat atcacgttct gtcatcaaat taagttcatc tacagttgtt
    13741 cgtttatttc cattttcttt agtaatttgc aaataaatat tacgaactaa gtcactcagc
    13801 gtctcgattg agagcaaatc atatgctgca ctgttatata taattttgaa cccgtgttga
    13861 tgtggaaaaa tttcaatatc cgctaaagat gtatgtgcat catgtatttg atgtacatcc
    13921 tcaataacat cattacaaca agacatcatt tgatgataac aatgaaaaat agtctctaat
    13981 gaaagcgaag acttcgcgca ctgtaattgc gacatatttt gcaacacact tttattaaaa
    14041 tctgttgtaa agcgttgaca tacatctttt gcatcgattg ttaacgttaa tggcacaata
    14101 tttccgtgta aatcatttgg taaatgtgat ggtacatgta tacctaatgt gacatcatgt
    14161 tgttgactca ttatatgatt agctaaatac acactaacag ccaacgatgc catatctatt
    14221 gatgtcatat catcaatcaa atacgtttga tataaagctt gctcaaatgg atgcttaatt
    14281 ggtaaataac tatcgatatg gatgtcagag ttattctcta gccgaaaata gtttgagtct
    14341 aatgctatat gcgatgcatc ttgattgtct ttatcatcat ttctatttaa atgtgcatgc
    14401 tgtcgagtat tgttaataac agtattgcca cgatatgcat tgcataaatc atcaagaaaa
    14461 atatcaattt gactatcatc gaaaatggac acatgaaaat ctaataatat atatgcagca
    14521 tcagcgaact gaaacaattt aaatttgaat aaaggtgaat cattaaaatg gtaagtactt
    14581 aattcttgct taaaaaaagc ttctaaatca tagtttgcgg aagaagatgg aacttgtttt
    14641 atctcaataa aaggcagaaa ttcatgaagt atcattgtta aattgtcatc ggtagtaaca
    14701 tcaaaaaaat gtcttataga tgcatgttgt tgcacaattg tcgataatgc atacatcatt
    14761 ttagtagctt caatattttt agcgagttta acccaatacg cattacggtg tgtcgttgat
    14821 tctgtattat ttttgtatat acgaaaatat tcctgttgaa atctcaaatt gcccataatc
    14881 ataaaaagtc cttctttcat atcataatac tcattactta ctgaaattgc atgatgatat
    14941 gataaccgac gaaatgttaa ttaactcgtt atgtaatgat taatataaaa caccattcgc
    15001 aacatatgag cgatatattc taccctaaaa tacatcttgt atcatcgtta caattggtat
    15061 atttttcaat gtaaattaca tacatattcg ataactagca cactacaact cgttaatcac
    15121 tttctcttat tcatatctca ttgcaagttc aatattgttg ttacaaaata tccatgaagc
    15181 aagtttatat taaacaaaca actcgcataa aacaattgtt atccttaaat tttaacaaat
    15241 tcttaataaa tttatctcta ttttaattac gaccaaatta ataggttttc aatataaaaa
    15301 gatgcataga ataaatattt aaataaattc aatttgtatt cacttgtttt tgtcccccga
    15361 atacaccagc aacaagcatg ctagcaccaa ttgttaaaac tataaacata tacagtccca
    15421 tttgtaatgt cgttaagaaa gcacccaaca caatccctaa cctagctagt gtttctgaaa
    15481 aatgaatgcc taatgcatta actgcactat atgttcctct tttagcttta ggaataattt
    15541 taaagcgttg ttctgaaact ataggcgaat aaataatttc acctacagtc gcaattatca
    15601 taaaaacaac taataagcca aactgattaa gataggttag accactatag ccaacaatat
    15661 aaatcagcaa accagtgatt aaagcttttt taaaatctat tttcaataca attttcgaga
    15721 ttgaatacgt gagtaaaatg acgacgaccg tattaatcat tagcaagatt gctaacatct
    15781 tagcacctgt aatatcatat gaacctatac ttattgtttc aaactgatcc tttagtctaa
    15841 tagcaatata tgaggagatt gaaaattcac ccatcatgat gatactgaac cccgaaatca
    15901 ataacatata attacggtct ttcaaaacta atttataact gcgaaatata ttcattattt
    15961 gtaatttttg ataacgactt gcatgtttct tgtcatcact ttgctttact tgatttcggt
    16021 cttgaggtaa ccaaatatat aaaataaaca gtacaattaa aaatatacaa gctgctatta
    16081 agaaaagcag taacatactg tagccataca tcaagccacc taacaatgct ccaatagcta
    16141 ccgataagtt cgtcatccaa tagctaatct tgtaaatata atgttccaca tcttcggtaa
    16201 ttgcatccat aattaacgtg tccataactg gaaattgcaa tccccaaaca attgtaaata
    16261 tggcgtatgc aacacaaaaa ccaataattt gccacaattg atgtgaccca aatatgccca
    16321 tgaacacaag cattatcacc atcgtcgctt gataaataag tactagcaac tttttcggaa
    16381 atatctcaat aaggtaacca gatataatag acaatggaaa ttttagaacc actaaaccaa
    16441 caaggtatat accgacaatt gattgactta acatatctgt taaatatagt gctataaacg
    16501 gtataaatgc tgtcgtaata attagctgta aaaaattgct aatcaatcgt actttcaaag
    16561 tcgtacttaa acgtttcatt gataactaca cccctatcca catatatgca cactaccatt
    16621 tatataaaat ctattatttg attatacgtt gaagcaaaga atttttagtt atacaaagtt
    16681 atttaacaaa caaatgtatc gactttacaa tgcatcttgc atataatgcc tcaaaaaaat
    16741 tagcactata tgactatata ttattccaaa tgagtgaaat ttatcaacat ttgcgtaagt
    16801 gaacacatat gtgcgtaaac gttaaatcat tgtcctaaca tagccgtgac tatacttaga
    16861 tgtaatcata aaaaaactac tgccatcttt acaacagcag tagtacttat atgattcatt
    16921 tatataaaat tgaaagcgtt aatcgctagc ccagcatcct tattctattt agctgtataa
    16981 cttttactag caatacgacc acttgcaaca ataatatctt tatcttggaa aggttcatga
    17041 ttgaagaaac gctctaaaat atctttaact ccatcttcaa tacgtttttg tgcttctaaa
    17101 gtcatacctg aatagtgaac tgtcatagca tttctaggca ttgttctcca tggatgatca
    17161 gcaggtgcag gttgtggata ccaaacatca ccagcatatc cttgtaaatg ctcagatgct
    17221 aacgcttcaa ctaacgcatc gcgatttaca attttaccac gtgcagtatt cactaaataa
    17281 ctgtgttttt tcatacgact taaaacatct ttatcaaata agttatcagt ttctggtgtt
    17341 aatggtgcat gaattgtaat cgcatcactt gttgaaacaa gttcatcaaa gcttacaaat
    17401 ttagacaatt tatggtcttg ttgattgatt ggatcatagt gttgtaatgt tacattaaat
    17461 ggcgctaatc tttcagcaac aagttgtcca attcgtccaa atccaaaaat accaattgtt
    17521 ttgtgttgta attcatgcgc atgattacct acttgagaca agttccattc accttctact
    17581 gattgacgat gaccttcttc atagtttcta agaagtatta ataaatccat aactgcatgt
    17641 tctgccacac taactgtatt acttcctgta acttcaacga caccaatgtt atgttcactt
    17701 gctgccgcta aatctacatg gtcagatcct acacctgctg taattgctaa tttcaagttc
    17761 ggtgcttttt caatacgttc acgagtcata tatgcaggat aaaatggcgc actaataatc
    17821 acatccatat ctggtaaatg tttatctaag tcttcaccat tatctgctaa tataatgaac
    17881 tcttgtcctc tttcctctaa aaatgttttt aatcctaatg cttttttagt attaagtaat
    17941 tgattgtctt gaccttctac tgcttctgga aataacgcta cgattttcat atccatctct
    18001 cctaaatgat aaattttgac ttccttaaag tacgtgttgc atgttgcttg ttttaaaaat
    18061 atgacaaaaa aaacggcact gtttgacata ccaccccctt gaattattgt taaattcaaa
    18121 gcgaagttag caaacagcac cgcgtacctt acaccagcaa cacttattct attgacttat
    18181 cgaaaacaaa tattgaaatg acttcatcat cattcaaatc aatatctgta aataatttta
    18241 caaacttagc gcctacaaca ctttccatct catttacatc tatttcttta tacatctgtt
    18301 taatcttctc tgtgcgtgta taatggagca tcgattcatt gcgtttgtcg tttaggtaaa
    18361 aactctcaac tttactcaaa acacctgtca tatgtgcaat cgcccaatta tctttaaacg
    18421 acactcgaat actgttgggt cctttaccca cgtattcttt acgataagat cttactagat
    18481 tcgagaattg ttgttcttta gtatgcgtat ctatagtatt caccttctct aaaataagaa
    18541 gttcattaat tgttcacatt ttcattatac accttattaa cactttttca atgataacga
    18601 ttattattac gaaatgtata ttttcctagt taaaaggata taacaaatta acagaaaatg
    18661 tttataaact aagatttata ttattttaaa aattgtttca acaagcgcat ctaaacaaca
    18721 aatcagcata tcatacttac cccttcaact ctgcctcaag tttacctaaa tgtcttaatg
    18781 ttggcgttaa tgccgcaaag aagaatcctt cttttaattt acgtacttgt ggcgaatatg
    18841 gtgtgtacgc tcttgaacca ccattgacaa cagaagcttg atttacatct aacaataagt
    18901 agccaatgtc cttcttcaat gatattaatt catttaaatg tgaagttaag ttaccgtcat
    18961 ctaatattgc ataatattcc tctctaagtt gacgatagcg ttttttaaaa ccttcaacat
    19021 catactctaa atattgattt attccgtttt gcgcatttga aaatgcatca attaactcta
    19081 aagaactttt aattgagcct aatcctattg gaatttggta agcaataaat tgcgggcgaa
    19141 tagttgccgc aaactgcttc gcatcatgcg tgataatttg tgattgtggc actgcgactt
    19201 gattcaaagt aatttgatac gttgctgagc cgtttacccc taaaaaattt gttttttcaa
    19261 caagcgtgat accatcttga ttggcacgta gaatgaacat gacaaattca tctgatgatt
    19321 catgcttcga aatcgcacca aaataatggt cttcttgaat attacttaca gctggcatac
    19381 gtccactgac aaccaattgt ccatcaacat aagtgtgttc aaggttcaac ttttctaaat
    19441 cattaaatga cttcatcgga ttagacaatc cggtagcacc taatatttct ccagataaca
    19501 attgctgttg taagtcatta tttaaatgtg gctgtgtggc attttctaaa tacgttgaaa
    19561 aagccaattg gcaccataaa caaaatcctg ttgtcaaaca agactgcgat acagcttcga
    19621 ttacttcagc attttttctc agtgtcgctt caccaaaata gccatctaca aataattgtt
    19681 ggataaatgt tctcggataa tacgtccctt catcaacttc tactaaatga ggatctaatt
    19741 gttctttgat aagtgtttct aaagtcatca tcatctctcc ttattcacca aatcttctaa
    19801 gtataaactg ctcaatataa ctaatgaatc gatcaataat aaaaccaaat aatccgataa
    19861 agaatattgc tgctaaaaca tcttctaagt tcaacatatt tcgtgcatca acgattaaaa
    19921 aacctaatcc cgattgtgca ccaatcattt caccagaaac taaaaatatc caacttgttc
    19981 ctaccgccat atgtatccca gccatgattt gtttaaatgc cccgggaaat aatatattgc
    20041 gatacaatga ccacccagtt aaatttaaat ttgccgctat ttttaaatat tgaggttcaa
    20101 tgtctctaac gcctttaatg gtattgaaca caattggaaa aaaagcagcg ataaaaataa
    20161 tcgcaatcgc tggcaaacta ccaataccaa accatagaac aacaaatggt gcccaagcta
    20221 tcggagatat cggcctaatc aattgaaata gcggttcgat agcgttgtat agccaacgat
    20281 tccttccaag caagaagccc aatggaatag caaccaacaa tgcgacaaca aagcccgcta
    20341 caaatctcca taaactaatt gctaaatgtt ggaaaatttc tccagtaaca atgaaagtcc
    20401 atatgctttt tcctacaaga gcaggaccag gtaacaatac aggttggtaa tgcccaataa
    20461 taatgaccat ttcccaaatg cctaagaaaa taataaatgt gataataggt aatataattt
    20521 tattaattgt gagacgtgtc atgaacgtga tgcctcctta tacaatgacg gttcaacaaa
    20581 gtcatcatat gcaggtggat taaacaaatg atgttgtttt accaatgtcg taatttcttg
    20641 atatccggat ggcttaattg ttaaatcacc ataggatgtc catgccgctg actgtgttaa
    20701 aacgtcacga ctttgtttaa aatgatgcgt cataatgtct acactttgct tgcgatcatt
    20761 cattttaaag ccagactttt tataatcttg tacaaatgct tgcgctacag ccttgtgttg
    20821 atcaagcaat tcgcctctca gtactagcac acagcaatac gcatcaggta taacgtcatc
    20881 accatgtttc aaagtcttac ctttgcctaa cttttccccc agtgcaccga atggttcggc
    20941 tacagaatac cctgtaattc tgtgttcact taatgcggct ggcatttctg ctggcgacat
    21001 ttcatgatag ctaaaatgcc ccggtttaat ctttaattgt tctcgtaatg cttcaagtaa
    21061 gagataatgt gttgaataac gatgcggtat accaaaatgg taatcatcgc cattattatt
    21121 aaattcattt aagtgcatac ctttttgtcc cataatgaca ttgccttcat gatgccccaa
    21181 tgccacagct tttatattcg agcccttctg ctttgatttc atcgcgagct ctattaaagt
    21241 tgatgcacca tcaatacgac cactgtttaa tgcgtccatt aaatctggcc aattattgaa
    21301 tttaactaat tctaatttat atttcggatg attgtgttgt gataataatt ttttagtcat
    21361 catcaaatta gctgaatgtg taatcggcaa atatccaatt ttaatcactt gctgatgttg
    21421 ggcattatta gacggttctt tagacgccct ttgccaatca catcctgtaa ttataaagat
    21481 tccaatgatg acgattatgc ttaacctttt catcgtcact cactccttat aaataatatt
    21541 caggttcaac ttgatgatga ttcaatgcaa atgtttccat aatttcatta cgaatcttaa
    21601 gtaggtggct atcattacga ctgcgtggat gtgatgctgt aatttcatat tgagaaataa
    21661 tattgcaccc ttcacctaac agaacaatgc ggtcggaaag ataaatagct tcatcaatat
    21721 cgtgagcgac taagataata gttgattccg ttttatgttt tagttgtact agttgatcct
    21781 gaagtttata gcgtgtaaat gcatctaatg cacctaatgg ctcatccatc aatataacgt
    21841 taggcttatg cacatgcgct cgacatagtg ccacacgttg tttcataccc ccggacagtt
    21901 gctcgggaaa atgctttcct ctgccttcta aatcaactaa tttaagctgt gcgttaatct
    21961 cttcatcact aattttctgt tggaatccaa tcctaatgtt gtcattaatc gttttccatg
    22021 gcagcagatt atgatgttga aatagcatta aacaatctgg agacggctgt tgtttaattt
    22081 cgttatcaat aatgacacga ccagatgatg gatgaataaa tccaccgatg atattgagta
    22141 aagtagactt cccgcaacca cttttcccta taaaagtgac tatttctccc ttgctaatgt
    22201 ccaaattaaa gttatgaatt actttatgtg atccaaagtg atgttgtaat tgttgtattt
    22261 taatcatgac aagccccctt ttaatcccta ccattttaat aagaattaaa tgtatatcta
    22321 aagggctatg ccaaacacag cccttaaatc gtgaattgtt atatctatag atgattatgg
    22381 tttggtatga gtactcttat aacataagcg ttattcaatt aacgacgtta tattaatttc
    22441 agctctacta aatttcaata aaatgattgt tttagacaaa acttgcattt agttcattag
    22501 tttcgtcata tcaactactt acatttatag cattgagcta ttctaattaa ctgcaatcga
    22561 aatccacgcc aaacctatat acatatatat catcaacttg ttgtcttatt aaagtttttc
    22621 gatgccatca atcggtgtat caactgcttt gcgcgcgcga cgtaccgctt cttcatcagg
    22681 tgcgttgtat aaacaaatac atttagacat atcttcacat acatatgtgc gtttaaattc
    22741 aacttctggc acttcttcat aatgaacaga atttttcttt ttacgtgcta aatattgatc
    22801 catcgtaatg ccttccggaa tgttccaagt tacaaggtaa tcaacatgtg catcaccatt
    22861 ttttacttca tctaaatctt taccaactaa gcgtacttct ttgactaatt gaatgctaat
    22921 acctgcttct gttaaaaatt gtttagcttc tgcttcgtca tttgcttcca caataaaata
    22981 accatgagtt aaattttcag tagcttgtac ttcaattaat gttggaattg tcttcgtaga
    23041 tagtgatgat gcctttgctt ctagttcttc tttcgttgat gcaaaatcaa gattgttagc
    23101 ttctaataaa aataatgtca ttaataagac ctcctataac aatggttgaa aaaaatatta
    23161 ctataattct tatcggatta caaggatatt gagcgatttt tttaaatatt tagataaaat
    23221 tctgtaaaat atattttcaa agagtataaa aataatgtta gggtataagt aaataaagat
    23281 atatataggg ggctatcatt atggataaag aatctgtcgt agcaagttta gcaagaaaca
    23341 aaaagattgc tgtagaaaca atgaccggtc aaaggtacat catagaacgc attttacata
    23401 caaatgatga aaaacatatt catattttga aacctaaaga tgtcgtgtta gatgtagata
    23461 ccatcaaaga catcgacgaa aatcatttag atgatgcaac ataagtataa aacgaaacat
    23521 gcgcctatga cattgattaa tgttttaggc gctacaatgt tttatttatg ttagtttgtc
    23581 gaagtatggc tcatctaatg gattcaattg caagttttca atttgcaacg ttgaatgtgc
    23641 aatgccatat ttttctttca acaatgatga tacttgatca atcgcttgat aatcatcccc
    23701 ctcatatttt ttatctaaca caacatgggc acttaatgaa taatgttctg ttgtaatact
    23761 ccacaaatga aattcatgta catctaatat gccatctatg ttttttatat ctgctataat
    23821 ttgatcagta tccaaatgtt gaggcacact ttccattaaa attaaccacg cattacgcgt
    23881 aattttataa ccaccacgta aaatgatgag tgaaattaca atactaatga ttgagtcaat
    23941 gatgcgccat cctgtaaagt agatcaatac aactgcaacg atgacaccaa tagagttcaa
    24001 taagtctccc atgaaatgcc ataatgcact ttgaatattg atattgtctt cttgttttaa
    24061 agaccttaca aggataacag tcaaaataat attgacgagt aaaccaatac tagcaatcat
    24121 aaacataatg ccactttcaa tgggttgcgg ataaataata cgtacaatag cttcatataa
    24181 aatccagatt gaaattacaa ttaatgctaa accatttaaa aatgccgcta atatctcaaa
    24241 tcttaaatat ccgaatgtgt agcgtgcagt cggcttctta ctagcaaaat aaattgccaa
    24301 cattgacaaa ccgagtgcta atacatcact aagcatatga aatgagtctg acagcaatgc
    24361 taatgaatta gatactaaac ctccaacaaa ttcaatcact gtaaataata gtgtgatgat
    24421 aagtgatgcc catagcgtct ttttagaact ttgttgaatt ttttgatgat cgacatgatg
    24481 aaaatatttg tatctgttta tcgtattcgc ctttttcccg ttcatagttt gccactccta
    24541 aataaatgcg tcgtatttaa tgtagttgct tttaataaca attatttata gcttctcagg
    24601 ttagtattaa tgcttccgta attaaccata atttatacat catcgtactt tacatgacaa
    24661 ctcattaatc tttacccttt atacctttat gtaatatcac cttggaaaaa cttttacagc
    24721 acactcgcgc tttgctttat ccctatcata tagaaatttt taaacatttc cgatatgatt
    24781 gtgctaatta cataaaaatg acaaaaaagt ttctcaaatt catttgtttt atttcatcaa
    24841 gtccaaaaat aacaatagta ttttatgatt gttccacata ttaaaacaaa cgatacattt
    24901 gcatatagaa cttcatattt caaaatcacc ttaattcata aacaactctg tccttataat
    24961 agccgcatcg caaattagca taagtaaaaa cagcagtaag atgatttgca attaaaaacc
    25021 atcatactgc tattatttta gtcattatat cctagtatca ttatgcaagt tcctaaatat
    25081 tttgtttcgc ttaaaatcta aacaactccg taaacatgct aagacttacc gctagactgc
    25141 ctacaaacac gcttcaaaaa tgatatttat tctagtacaa accttttaaa gcattgcttg
    25201 tatcaatata aatattttta acttgttgat agttacttaa cgcaccttta taagtttctc
    25261 gaccgatacc tgatttttta taaccaccaa atggtgcgcc ttctggtact tggttgtaag
    25321 tgttaatcca aatacgtcct gtacgaacag ctttagcaat atttaatgca cgtgtgatat
    25381 tttgagaaaa tacgccgcca gctaaaccat actctgaatc attagctata tcaattgctt
    25441 cttgatcatc cttcacttta atcactgtta aaactggtcc aaatatttct tcttgcgcta
    25501 atttatgatg attatctggc acagcaatta acgttggttc aaagaagaat cctttatcta
    25561 acccattttc agttaaacga tgaccacctg ctaaaatttg tgcatctgat tcttttgctg
    25621 catcaatata tgattgaatt ttatctaatt gatccttacc agtttgacta cccatttgtg
    25681 tagcttcatc ttgtggatct ccaactttaa tatttgaaaa tgcctcttgt aaacgtggca
    25741 ccaattgatc ataaattttt tcatgaacta ataatcgaga acctgcacta catacttcac
    25801 cttggttgaa taaaatacct aactgaatac cttcaactgc aaggtctaaa ttagcatcat
    25861 ctaatatgat attggcgctt ttaccaccaa gctctaaagt agcaggtact agatgtttcg
    25921 ctgcagcttc ggcaacttga taacctacat cagttgagcc cgtaaatgat aatttatcta
    25981 ccccatcatg attgaaaatt gcattacctg attctgaacc tttacccgtt agtatattga
    26041 caacaccatt aggtaatacc tcttggaaaa ttttagcaac ttccaataaa cttaatggtg
    26101 ttgaagacga aggttgaatc acaattgtat tacctgcagc aatggctggc gcaatcttcc
    26161 atgcagctaa tagcattggg aagttccaag caacaacagc acctacgacg ccaatcggct
    26221 catgtcgtac gatactcatt gtgtctttat cgatatcatt cactgtacct tcttctgttt
    26281 caataacact tgcgaaataa tggaaatgtc ttgcagcaaa tggaatatca attgctgttg
    26341 tctcacgaat cggtttacca ttatttaatg tttcaatcat tgcaatttta tctttttgtg
    26401 ccattaattt atcaccaata tcacgcaaca tttgtgcacg ttctgattta gaagttaatg
    26461 accatgattc aaatgcctct tgcgccactt tgactgcatg atcgacatct ttatcttttg
    26521 ctcttgtaat atgtgatagt gtttctccag ttgctggatt agtcacttcg attgtctcgt
    26581 cactgctacc tttaacaaat tccccattga taaataaacc ataattctct gctatataat
    26641 ctcgaacgtt tactgccata ttatctctcc tttgtttatt ggctataata taaatctctt
    26701 agcatcatat ttaataatgc ttttaaacga tatgattcac attatatagt aagcgtttac
    26761 attttataaa ataatcagct cacagaagtc tcatttttaa aacttttgcg tcgtttttgc
    26821 acataaaaat aatcttattt tcgaaaacat attgcacttt tcaaaaataa aacacataat
    26881 ataactataa tacttgagaa taattctcat taaatagttt ggttgttatg aaagtcgcgc
    26941 ttaaaatgcg ttaatgggac aaggataatt caacaacgtt tcatcttact gagtcattgc
    27001 acttatcata cacattatat ttagcatgag ttatattact aaaacaatat tacatgcgca
    27061 ttagtactag aatggagttt tttcaatgcg acttatttta atcgtcattg gcttaatatt
    27121 tacagcgctt ggtattgcag gtgccgtatt acctttactg ccaacgaccc cttttttact
    27181 cgtagcagtt ttttgttttg ctcgaagttc agatcgcttt tacaattggc ttattaatca
    27241 aaaaatttat aaagaatatg tagaaaactt ttatttacat cgaggctaca cgctacaaca
    27301 gaaaattaaa attttaatta gcttatacat tgtaataggt ttttcaattt atatggtgga
    27361 tgttcttgca gtccgtatag gattaatcat aatggttatc atacaaaccg ctgtactctt
    27421 tacatttgta aaaacattac ccaaatcaaa taataaaata gaggagtgat tgcccatgtt
    27481 tatggcagaa aatagattac aattacaaaa aggcagtgcg gaagaaacga ttgaacgttt
    27541 ttacaataga caaggtattg aaactattga aggcttccaa caaatgtttg tcactaaaac
    27601 attaaatacc aaggatacag atgaagttaa aatcttaact atttgggaat ctgaagatag
    27661 ctttaataat tggttgaatt ccgatgtatt taaagaagct cataaaaatg tacgtttaaa
    27721 aagtgatgac gatggacagc aaagtccaat attatcaaat aaagtattca aatatgatat
    27781 tggctaccac tatcaaaaat aaacatatta aaaaccccta gtcaccgctg gttgtctaaa
    27841 ccaacttaat gattaggggt tttttattat ttgcgacgta aaggtacgaa ttcatccggc
    27901 ttgtctgaac gcaatccaaa ataatattct attgcttcac aaatacgtcg tgatgcttgt
    27961 ccatcaccat atggattatt cgcttgactc atacgttgat acgcttcatc atcctcaatc
    28021 aattgtttca catttcgaac aatattgtca taatctgtgc caattactct cgatgttccc
    28081 gcctcaacgc cttcgggacg ctctgtatga ttccttaata ccaacacagg ttttccaaat
    28141 gtaggagcct cctcttgaat accaccagaa tctgtcagca cgaggtacga ttgatttgta
    28201 aaattatgga actcaagcgc atctaatggc tcaattaatt caatccgatt tctcccagat
    28261 aaatattttt cggcaatcgc tcttaccttt ggattacgat gcattggata aatgaagaca
    28321 acatctttat attcatctgc caaatctctt actgctttaa aaatctgatg catcggttct
    28381 ccaatatttt cacgacgatg cgctgttagt aaaataactt tcttgccttt atgtttatta
    28441 ataatcgttg aaacaaactc attttgaact gttgttgaca atgcgtcaat aactgtattt
    28501 ccggtaataa agatactctc tttgtctttg ttttcaaaaa gtaaattttt agctgcaatt
    28561 actgttggcg caaaattcaa ttcagcaata ttacttacca tgactcgatt taactcttca
    28621 ggaaatggtg agtatttctg atgtgttcga agtccagctt ctacatgtcc gaccggaatt
    28681 tgatgataaa atgctgccaa gcttcctaca aaagtcgttg tagtatcacc atgtactaaa
    28741 atcatatccg gctgttcctc attaatgatg ctatcaagtt tagcaagcgc attcgccgta
    28801 atgcccgcta acgtttgttg atcttgcata atatttaaat catgatcagc ttgaatatca
    28861 aatatactta acacactatc taacatatct ctatgttgtg ctgtaatcac aatgttcgct
    28921 tcaaagttcc cattatgatc aatttctttt actaatggtg ccatttttat tgcttcgggt
    28981 ctcgtaccga aaataaccat aatttttttc ataacattat cctctctgaa gttcaaacac
    29041 atgacagctt atctttcctt aatcatgtat gccctagttt gacacatttt atttgtcgat
    29101 aaaattaaat atattgccat aattataata cgatacatct tcaaatgatg atttcacaac
    29161 attttttgta tcaaaaatca ctttatgctt cattttatca aaatgactgt ccgataaatt
    29221 tttaaattct gagtggtcac ttaaaatcaa tactagcgat gcgtctttga cagcatgtga
    29281 catatcatgt tccacaaaat ctaattcaac atgtggatca taagcacata cttctatgtc
    29341 tggttcttga tttaatagct cataaatatc aaatgctggt gattctctta tatcatcaac
    29401 atcaccttta taagttaaac caaatactgt gactttattc ccgctcaacg ctttgatgat
    29461 ttgcttcgtt gtatcaacaa cataggacgg cattgaatta ttaatttcac gtccagtttg
    29521 aattaacttt gcattttcag ggtctttagc aataataaag tacggatcaa cagctaaaca
    29581 atgaccgcct acacctggac caggttgatg gatattaaca cgcggatgtt tgtttgccat
    29641 ttcaatcaca tctaatacat taatatttaa gttgttgcaa atttttgtta attcattagc
    29701 taaagcaata ttcacgtctc tatatgtgtt ttccattagc ttactcattt cagcagtacg
    29761 tgcatctgtt tcaatcattt ctccctgaac gaatgtgcga tagacacgtt tccccgcttc
    29821 aatacaagct ttagtcacac caccaatgat gcgattgtta tgaactaatt cttctaaaat
    29881 ttttcctggc agtacacgtt ctggacaatg cactaaataa atatcttcac ctattgtaaa
    29941 ccctaaattt tcaatgactg gttttacaaa atcatccatc gttttaggcg caattgtcga
    30001 ctctacaata atggtatttc ctttttctaa aaatggtaaa atactatcta atgcacgcat
    30061 aactagcgaa atgtcacatg accggtactg atcatcatta ttcggtgtcg gaacggcaat
    30121 gataaaaaca tcagatgctt ctggcgttgt agataccttc aattttcccg atgacagtac
    30181 ctcttcataa acctcttgta atccaggttc ttcaatacta atttgaccac tttgtaactt
    30241 atcaatcgtt tgctgattaa tatcaacacc aagcacatcg acgccatgct ttgcaaacat
    30301 aattgatgtt ggtaaaccaa tataacctaa gccaactact gttaacttca tactataacc
    30361 tccaaataaa acgatttgta tgttgatttt atttaataga tgacttatgc tttatctttg
    30421 gttgtcgttt catccatgat gtccgcaata acaagtgaca tttttccagg aataacttca
    30481 agcgcattat tattttcata taaagtattg ctgtatgtta aattgccgaa taattttcta
    30541 aagaccgaca acttattaaa atacttattt agcattgaag gcatgttgat caatagcgtt
    30601 ttacgatgcg attggcgacg tatttcatac attactgacg atgtatcaaa gtaaaaacta
    30661 tcttgaggat gatacacgcc tgtcacttct aatgatatta attgatcaat aaatgctgtc
    30721 agatgtttaa tatataatgc actccgctga ttattaatat ttggaatgat tggcaatcgc
    30781 tttgacaatt gcattaaccg ttggaaattt cctgggcaat gtgcaccata aatcattggt
    30841 ggtctcacaa ttgctacttt aaacgaatca ctaatcaact cttgtaatgc ttgttcagcg
    30901 aactttttgg aaataccata gttggtcgta gggttcattg gtgtttgtgt atcaatttga
    30961 tctgatttac caacctgacc ttcttttcca taaactgcca tagtactcat aaaaataaat
    31021 tgtttaacgt cttcagcttt agccttttgt gccaattgtt tcgtaagcaa catattcact
    31081 tgcatataat cagatagcct tgcttgaggt gaattgttgt gaaccaaagc tgctgtatga
    31141 attaaaacat cataaccttt gaacgaggtc gacttccata attgattcct aacattaatt
    31201 tgatccactt gatgtccttg ttcaataagc ttatctttta aagcattacc gatatatcca
    31261 tgtacgcctg taattaaaat attttttctc attagtgatg cacaccttct gaagtaacga
    31321 tatttttaat tgttttatat atgatataca tatcaagcat catagattga tgtgttaagt
    31381 aataatgatc atacgctact ttttgatcat cagtaatatc atctctcccc atcacttgag
    31441 ctagccctgt tacacctggt ctaatcgtat gcacgttcgc ttttgtacgt ttttcaatta
    31501 attcgtattg attataaagc gctggtctag gacctacaat tgacatttct ccttttaaaa
    31561 cattcaataa ttgtggcaat tcatcaatag aggttttacg aatgaccttc cctgtctttg
    31621 ttatatacgc tgttgaatcc attaaatcag ttgcaacatt aggtgtgtct attttcattg
    31681 atctaaactt ataaatatta aacaattcat tattaatcgt cggtcttttt tgtttgaaaa
    31741 tggctggtcc gggtgattcc attttaatta gtaatgctgt aattaacaga atcggactta
    31801 aaactactaa accatatatt gaactcacta catcgaataa tcgcttcata attatctcct
    31861 cacttcaaca gattgtaata cttcatagta ttaatttctc tcgtatattt atctttaaac
    31921 agttgatgtg cgcgctcacc catctcgtgc aattccttac tcgagtgagt gttgatgaag
    31981 ttataaatgg catgtgcatc gccattatca atttggatac ccgcatcata ttgttcaaca
    32041 tgttgaacga tatcagattg cttatccatg atgagtacca acggcttctt agctgcaagg
    32101 tagccataat tcttgctcgg cacgcctaaa ccgacgcctt ctttaatcag cgatgcaata
    32161 catacatccg caatttttaa tacgtcagca tagtctgtac ctgttaaaaa ctcaaacatt
    32221 ttaacatttt caatacgatg gtcttctatt gccgttttga catctgcaaa tttcttacca
    32281 tgaccacaaa gtattgttaa cgtttgaggc tgatccttat ttaattttaa aaatgagata
    32341 agtgtctcca tatcctgtaa ctgccccata ttaccgctat acaataaaat tttgtcgtat
    32401 tgctcacggt aagctttaaa tgtgtcatta tagatacgat tgtcttgtaa ctgacgcatg
    32461 tcataccagt taggaatcac atgaatattg tcaggatttt tagaaatttg atgatttagt
    32521 aagtagtttt tcatttccgt accaaggaca atgacatttt cagcattctt gtagacatgt
    32581 ttattaatgt aacgcatcag tttatcaatc atgctacctg gacgtgttgc acctgtctta
    32641 atcgcattat caggtgctat atcatacacc acaaaagaat attttttctt aagcaatctg
    32701 tgtagaacgt ctggtattaa tggcaagatt ggtggattag agtaaacaag aatctgatca
    32761 tatttcaaca ttttaggtat attaatcacg aattttgaaa ataaactaaa gaaattgatg
    32821 atccttccaa ccttactttt gttattaaac ctcgaatact tgagacgtcg aatgcgaata
    32881 ccacgatgca tctcggtttt agaaacctgt ttatgattac tatattcata tggccatcca
    32941 cacatgacat cgacattaat gtgattcgca attaaatctt ccgccaattg tgttggtaac
    33001 gtcgctgaag atacatattc cggataaaaa tactgacata aaatcaaaat ctttttttca
    33061 ctcatctaat cgacgtcctt tttattaatg aatttagact aagaattttt gaactgttta
    33121 tttttagcaa aataatagag tgctatgaaa aatatcatgt acgatacgat taaaacaact
    33181 gtattaatag atacaaagtt taaaaaagtt aaattaagca tttttgatat aacaaatgtt
    33241 gcagtagtac ctattaaaaa taacgcttgc agtattaatt ctaactgttg tttttgtaat
    33301 acaatcacag aaagtgatac cgttgataca atcagcctta taacaaataa tggaataaca
    33361 atttttataa ctgtaattgc gtcaacccag ctatgtccta ataatatatt gactaattct
    33421 tcactgaaca aataaagtgg cacataaaga attatagaaa ctataaccag tatggaacta
    33481 aatttaagta ataaatttcg gaagttacca tattcaatat aatagtcatt agcttgttgc
    33541 ataaatattt ttgataaccc taatgaaata attgtcactg gtatgcctag tactcttatg
    33601 gccataccat aaatacctac ttctgtattg gtatacaaaa tacctataaa gaacacaaca
    33661 accgagaaag acaaactatt taataatatt gatggtgttg aataaactaa ctgattttta
    33721 ttttctaaaa ataatgcttt agtttcttct ttatctctca caatattaaa gtgcttttta
    33781 aacgttttat atcctagtgt aatgcctgcg atataggata atgaaaaacc aataattaat
    33841 cctagacttc ctaatgctaa aagtccgaaa ataatttgaa gtaaagccat tgatccagct
    33901 ctaaacacat taatcaaact taacacttta tattttcttt ccttatttag ataatttgta
    33961 aaaatgtttg ttaaacttac caaaaacaac ataataataa tgaaaataaa atctaatata
    34021 aagtttgggt ataaccatgc actaatcgca aatattggta tcagtattaa taaaataagc
    34081 aatgatatca aaaaactgat ttgtgaaagt atattagcac tacggtcatt tttagtattc
    34141 acaatcaaca aatcgtatct tgcatttatt attggtatta aaatcattgc gatatttgaa
    34201 aaaattgtaa actcaccaaa ttctgtaggt gaatatagtc ttgtaataat tggggtagtg
    34261 atgattaata tgacttgagc gatgccactg cttaaaatca ttaagaacga atcgccaata
    34321 aatttattta atctcataat taacctcaga ttttcttgat gactttcgct ggattgccac
    34381 ctataacaac gttaccttct ggaaaacttt ttgttacaac cgacccagcc cctacaattg
    34441 tatgttcccc caattctact cctggtaata taactgagtt cattccaatc caactataat
    34501 tccctatttt gacatcttca cctggtgcat gtgacttcaa atttttaata ttatgattag
    34561 ctgtaataat acctacgttt ggcgctatat aaacacctct acctatatat ataactgctg
    34621 aaaaattatt aaaatacgtc ccgggcgatt ggaaaatatg aatatcattt ttatcaaaaa
    34681 caatgttatt agggttatgc attctaacag ttatatctgc aggaaatggc aagctcgtat
    34741 ttattcctaa caacttgaac ggaacagctt tccatgccca taaccatcca gccactttat
    34801 tttcaaaata atatccagtt aaatattctt tcttataaaa taataatagt ggtgtattca
    34861 ttaatctaaa tattttcgat ttcataaatg tttttacaat cattctatcc ctactttttt
    34921 agtaaatttt tttattataa tgcctgggat aatagctata acatatacaa ctaaaatcat
    34981 agaaatcaat gtgattaatg gtgtcgggtg actaaaaata ctcgtaataa gtctcatata
    35041 aatatatgta ccaataaata tcacttctaa cgaatgactc ttccgaataa tatactcact
    35101 cagaaatgca aaaaagatat ttgcaattaa attcaaatag aagaataagg gtccaaaata
    35161 aataattcca tatccagcac tcgaaattag atgaccagtc aattgtttac taccatatat
    35221 caattggtta aaaagttggc tagtaatcaa ctgcttttta tctacaaaga aattaaaccc
    35281 aaaaacagct ctagtattat caaataaata ttgtttaaac gaaccattat agtaattcaa
    35341 atagtctata gaagctgcaa tattgctagg tccataaaaa tatgactgca acgtgtcaac
    35401 tatcttaaga ttacttacac tcgtactatt taacgcctcg ctatatgaac tataattaaa
    35461 cacatacaat tctttatata gtgtcatcaa tgctaatacg aaaataccaa cagatataat
    35521 aatgatattg atacgtcttt tgtgcttaga aaacaagagt gacaccactg ttaaaacagc
    35581 aaccatcgta taaagttgat aacttcttct ttcaccaaca attaaactaa tatttaaaat
    35641 ccctatagct agcggcaata taacataaat aattttagga ttctctttat actttttata
    35701 tgaagcatat gcgattatta agaataataa cgctaaggca agttgaaata gcattacaaa
    35761 aaggacattt aaaccacttg ttgcttcggt tcctcttccc attgcatctg ttttaataat
    35821 taaaaatgat atgtttttgc gcacttcggg aaaagctaca aaaatcccac aaataacaag
    35881 accaaacaca atgtaataaa ttgatgatcc agaaagtgta aagctatttc ttgataccac
    35941 ttcatgtttg aataatttag atagtatcaa tataaatagc gagcctatga taaactccac
    36001 actcatataa ataaccgctt gattaaaata atcttgtcct aatggtaaat tatctatagt
    36061 tgaatcctgg aaaaacacta tcatagcagg tataaatact aatctaagaa aacctaaaac
    36121 atcgtataca attttcgaaa atttaaaatt cggattattt gagaagctta taaagagctt
    36181 tcctactcca taacttatag gtgctaaaaa caataaaggt ttatcaccta acataaggca
    36241 aggaaacatt aaaatggcaa taacatatga agtgacatta tatatcttat tcattaaata
    36301 tcctgcctat ctcttgatag aactgtttat tattttcaat tacatcgttt ctataagctt
    36361 ctcttttatg attaatgtcc attacattca aattgttata ccaatccatc aattcatcac
    36421 caataccttt taaattttct ggattaacac taaaacccag tccgaattta ttagcttctg
    36481 tagcggtgaa tgtatcatct gttgtaataa taggtttgtt taaaaacaac gcataataca
    36541 ttcttatgga taaagcagta tctaaagcaa tatttttatt accaaacaaa ttattaagta
    36601 tatcaatttc atttaaatat ttcggtgttt cttccaagtc aaaaccgcca gaaaatgtaa
    36661 tattattaat aaaattttct cgagcaaaga cttctaattt ttccgaccct gttccaaaat
    36721 attgcatatg gaaccttgaa tcatttgcca gttcctttat caatttttga tttatctcat
    36781 taaatctgac attaccaata aaacctatat taattggcca tctttttttg aattcatcgg
    36841 tcacattact ttctctaata atactcatgt tataactata aataattcta tatttttcag
    36901 atttaggtaa aaatttaaga aaaccttctg aagataatgt tgtcagcctg ctagcatcaa
    36961 cgatttt
//
LOCUS       DAKSQE010000014        35447 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.014, whole genome shotgun sequence.
ACCESSION   DAKSQE010000014 DAKSQE010000000
VERSION     DAKSQE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 35447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35447
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.014"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..83
                     /locus_tag="O0V33_000566"
     CDS             <1..83
                     /locus_tag="O0V33_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
                     /protein_id="HCX8975239.1"
                     /translation="FNNNEDYARYSEKYNDLILEFDNEDI"
     gene            complement(88..304)
                     /locus_tag="O0V33_000567"
                     /pseudo
     CDS             complement(88..304)
                     /locus_tag="O0V33_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789088.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(341..1081)
                     /locus_tag="O0V33_000568"
     CDS             complement(341..1081)
                     /locus_tag="O0V33_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCX8975240.1"
                     /translation="MFNLKHDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTMPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNACHKSYMKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRALNKIEDADTLVVLGSSLVVQPAAGLISNFKGDNLIIIN
                     KDRTPYDNDATLVIHDDMVSVVKSLMTE"
     gene            1287..2078
                     /locus_tag="O0V33_000569"
     CDS             1287..2078
                     /locus_tag="O0V33_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCX8975241.1"
                     /translation="MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIARKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            2445..2873
                     /gene="lacA"
                     /locus_tag="O0V33_000570"
     CDS             2445..2873
                     /gene="lacA"
                     /locus_tag="O0V33_000570"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HCX8975242.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            2889..3404
                     /gene="lacB"
                     /locus_tag="O0V33_000571"
     CDS             2889..3404
                     /gene="lacB"
                     /locus_tag="O0V33_000571"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HCX8975243.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            3417..4349
                     /gene="lacC"
                     /locus_tag="O0V33_000572"
     CDS             3417..4349
                     /gene="lacC"
                     /locus_tag="O0V33_000572"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HCX8975244.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHAGIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPKISVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDDLFNQIEVLEV"
     gene            4353..5333
                     /gene="lacD"
                     /locus_tag="O0V33_000573"
     CDS             4353..5333
                     /gene="lacD"
                     /locus_tag="O0V33_000573"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCX8975245.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKDQDASTHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            5355..5666
                     /locus_tag="O0V33_000574"
     CDS             5355..5666
                     /locus_tag="O0V33_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCX8975246.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            5672..7384
                     /locus_tag="O0V33_000575"
     CDS             5672..7384
                     /locus_tag="O0V33_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCX8975247.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            7402..8814
                     /gene="lacG"
                     /locus_tag="O0V33_000576"
     CDS             7402..8814
                     /gene="lacG"
                     /locus_tag="O0V33_000576"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCX8975248.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYQGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGEYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIKDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
     gene            9080..9949
                     /locus_tag="O0V33_000577"
     CDS             9080..9949
                     /locus_tag="O0V33_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCX8975249.1"
                     /translation="MKKIFVISTTLLAVAIIIGTIITVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLSKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGESAQHSQLHENE
                     NVANELIDFLWKK"
     gene            10232..11233
                     /locus_tag="O0V33_000578"
     CDS             10232..11233
                     /locus_tag="O0V33_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCX8975250.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEDIDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKDGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAESLAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPRAFKNLFTGDNFGKQVIKVTE"
     gene            11468..12475
                     /locus_tag="O0V33_000579"
     CDS             11468..12475
                     /locus_tag="O0V33_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCX8975251.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMEVTQAPRVLGFDAIGTVEAIGPNVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISHNPSENIGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEKEIPLVDY
                     IFCTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHR
                     TPDMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHLLLEKQSMIGKLVIN
                     I"
     gene            12783..14345
                     /locus_tag="O0V33_000580"
     CDS             12783..14345
                     /locus_tag="O0V33_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCX8975252.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITSFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYASITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLLGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPSGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIAEIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLLKSLSEEKPRNSNKVLIKHRDPDV
                     LEYRSSKHIDEN"
     gene            14520..15056
                     /gene="amaP"
                     /locus_tag="O0V33_000581"
     CDS             14520..15056
                     /gene="amaP"
                     /locus_tag="O0V33_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCX8975253.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRADIKKNVEHFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            15069..15308
                     /locus_tag="O0V33_000582"
     CDS             15069..15308
                     /locus_tag="O0V33_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCX8975254.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTILIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
     gene            15371..15880
                     /locus_tag="O0V33_000583"
     CDS             15371..15880
                     /locus_tag="O0V33_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCX8975255.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
     gene            complement(16041..18017)
                     /gene="sfaD"
                     /locus_tag="O0V33_000584"
                     /gene_synonym="sfnaD"
     CDS             complement(16041..18017)
                     /gene="sfaD"
                     /locus_tag="O0V33_000584"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HCX8975256.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDNITNMITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAMSYQALSMQHDSTPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLSRTMSLSKDYDTTVHQLFPDLIKQLENEFTPN
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGI
                     HFYNEQDEADYQTERSEQLGTLFRKNIYQMIPQEVTPMIPSSLVATYPFNNESPIVTL
                     IKRYQSAASLSDFESSAKSWIETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKAQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVHSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            18119..19312
                     /gene="sfaA"
                     /locus_tag="O0V33_000585"
     CDS             18119..19312
                     /gene="sfaA"
                     /locus_tag="O0V33_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HCX8975257.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQSICYFIAVLLCLPLHFKVTKIPEDATRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPRVLKYIGMVNM
                     YYLSSFLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            19299..21056
                     /gene="sfaB"
                     /locus_tag="O0V33_000586"
     CDS             19299..21056
                     /gene="sfaB"
                     /locus_tag="O0V33_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HCX8975258.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKESLIEIQY
                     HMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIMTLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAIDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYSQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEEAELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            21063..22133
                     /gene="sfaC"
                     /locus_tag="O0V33_000587"
     CDS             21063..22133
                     /gene="sfaC"
                     /locus_tag="O0V33_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HCX8975259.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNIQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISMADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     HINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFIQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNQY"
     gene            complement(22234..22359)
                     /locus_tag="O0V33_000588"
     CDS             complement(22234..22359)
                     /locus_tag="O0V33_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975260.1"
                     /translation="MYHGYIIFELIQLTPFSMTVISLIQLILPIAVLCDELLHLI"
     gene            22526..23509
                     /locus_tag="O0V33_000589"
     CDS             22526..23509
                     /locus_tag="O0V33_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCX8975261.1"
                     /translation="MRGLKTFSILGLIVALFLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIRDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNIDAFKTI
                     AKALDKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDNAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
     gene            23521..24552
                     /locus_tag="O0V33_000590"
     CDS             23521..24552
                     /locus_tag="O0V33_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCX8975262.1"
                     /translation="MAIKELSSQSAIDHKRKRRTTLTYMVSVCFLFICVYLNMAIGSS
                     KINFSDIVHYVTGHTDTKATFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFVIVLVTIIIPSLEYYSLYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWLIGALIA
                     TVFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSIAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            24549..25517
                     /locus_tag="O0V33_000591"
     CDS             24549..25517
                     /locus_tag="O0V33_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCX8975263.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MIFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
     gene            25753..27123
                     /locus_tag="O0V33_000592"
     CDS             25753..27123
                     /locus_tag="O0V33_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCX8975264.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLLHNAFNVRIGW
                     LILRLLAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGIVDFFKSGLKTVVDMWFVILPVV
                     MSIGTIATIIANYTPLFVILGKPFVPFLELMHIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
     gene            complement(27255..27515)
                     /locus_tag="O0V33_000593"
     CDS             complement(27255..27515)
                     /locus_tag="O0V33_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975265.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            27692..28207
                     /locus_tag="O0V33_000594"
     CDS             27692..28207
                     /locus_tag="O0V33_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCX8975266.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPKGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMIGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            28251..29438
                     /locus_tag="O0V33_000595"
     CDS             28251..29438
                     /locus_tag="O0V33_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HCX8975267.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQAKQLKELHRQTGHKIQWYIMTSDINHEETLAYF
                     ESHNYFGYDQESIHFFKQDNIVALSEAGQLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVKVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
     gene            29457..30140
                     /locus_tag="O0V33_000596"
     CDS             29457..30140
                     /locus_tag="O0V33_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCX8975268.1"
                     /translation="MSKSSEKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIAYGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
     gene            30318..31661
                     /locus_tag="O0V33_000597"
     CDS             30318..31661
                     /locus_tag="O0V33_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCX8975269.1"
                     /translation="MRLKSIVTVIALILIMFMAAIESSIISLALPTIKQDLNAGNLIS
                     LIFTAYFIALVIANPIVGELLTRFKIIYIAIAGLTLFTVGSLMSGLSTHFSMLVISRV
                     IQGFGSGVMMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWH
                     WLFYINIPIAIIAIILVVWTFHFPEEETVAKSKFDTKGITLFYIFIGLIMFALLNQQH
                     LYLNIIGFVLAILVALRLFNVEKKVSSPFLPVAEFNRMITLVFITDLLTAVCLMGFNL
                     YIPVYLQEQLGLSPLQSGLVIFPLSVAWITLNFNLHHIEAKLSRKVIYLSSFTLLLLS
                     SIIIAFGIKLPILIACVLILSGLSFGYIYTKDSVIVQEETSPIQMKKMMSFYGLTKNL
                     GASIGSTIMGYLYALKSGLFGANLHNILGVVSLISVGLIVVWLIFYREAASQTKE"
     gene            31783..32256
                     /gene="sepA"
                     /locus_tag="O0V33_000598"
     CDS             31783..32256
                     /gene="sepA"
                     /locus_tag="O0V33_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HCX8975270.1"
                     /translation="MVMNYLKHKFYNLLTTIIVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIVFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYMVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
     gene            32445..33888
                     /gene="lmrS"
                     /locus_tag="O0V33_000599"
                     /pseudo
     CDS             32445..33888
                     /gene="lmrS"
                     /locus_tag="O0V33_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
     gene            34036..35100
                     /locus_tag="O0V33_000600"
     CDS             34036..35100
                     /locus_tag="O0V33_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HCX8975271.1"
                     /translation="MLTVDQVKELIGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     VAMAQLGGAPQLDLQMAVVNVLKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGINGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVRWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            35189..35375
                     /locus_tag="O0V33_000601"
                     /pseudo
     CDS             35189..35375
                     /locus_tag="O0V33_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12079 a         5004 c         6621 g        11743 t
ORIGIN      
        1 cttttaataa taatgaagat tatgcaagat attctgaaaa atataatgat ttaatattgg
       61 aatttgataa cgaagatatc tgatttttta atctaacaaa tgacaaacca ttaaaagtag
      121 catcccaaca tcaaataaaa cacaaatcat caaataaaaa tcgctacaaa accaaatcgt
      181 taagcacgca ataaataaaa tttctcactc attttaattt ggaattccaa acgtcgaatt
      241 ccgaaaacca aactccgaat tccaagctca aaaaatgaat acgcaacttc aaaattaaaa
      301 gcatttccct gttactcggg aaatgctttt tacgtactga ttactctgtc attaatgatt
      361 ttacaacgga aaccatgtcg tcatgtatga ccaaagtagc gtcgttatca taaggtgttc
      421 gatctttatt gataataatt aaattgtcgc ctttaaaatt tgatattaat cctgcggcag
      481 gttgtacaac gagtgatgaa cctagtacga caagggtgtc agcatcttca attttattta
      541 atgcccgtat gatggtaggt tgatctaaca tttcaccgta taatacgatg tccggtcgaa
      601 tggcaccacc acaattatca caatgtttca aagttctatc aataacatct gacttcatat
      661 aagatttatg acatgcatta caataaaaac gatttaacgt gccatgtaat tcatcaacat
      721 gttgacttcc agcgtctgag tgcaaaccat cgatattttg cgtgatgaca cctaaagatt
      781 gttgattacg ttctaatttt gcaatccaat catgaacgat attgggcatc gtatcgacaa
      841 atagtaagcg cttatggcag aaattgataa aaccttcagg atcatcttct aaataatcac
      901 ggcttaacaa gtattctggc gaaagcccat cttttgaaat ttcatcaaat aagccaccca
      961 ttgaacggaa atctggaaca ccacttgcga cagatacacc agcacctgta aaaaatgtaa
     1021 tacgattcga actatctata atatgtttta gtgtctctaa atcatgtttc aagttaaaca
     1081 ccccatcaaa ataatgaatg atttataacg tcttttgctt ttattatagt atattcattt
     1141 tacgattatc aaaagcgcag tttaaacagt tctttttaaa aatgaattat acatataatc
     1201 gaacagttaa attatattag tttttacgca atttttatta tagaataagc atgtatatta
     1261 aaattgatgt ctaaacataa aggataatga cccatcgcct aaataaagga ggcaatttta
     1321 cattgaataa acatgaacgt ttggacgaaa ttgctaaact agtgaataaa aagggcacga
     1381 taagaacgaa tgaaatcgtc gaaggtttaa atgtgtctga tatgacagtt cgaagagatt
     1441 taattgaatt ggaaaataaa gggattttaa cgaagattca tggtggtgca cgcagtaatt
     1501 caacatttca gtataaagaa atatcgcata aagaaaaaca tacccgtcaa attgcagaaa
     1561 aacgatttat agctagaaaa gctgcatcat taattgaaga tggggatact ttgtttttcg
     1621 gaccaggaac aacagtggaa ctattagcag aagaagtcaa tcatcatacg ctcacaatta
     1681 ttacgaattg tttgccggtg tataaaattt tgttggaaaa acaaacagca cattttcgtg
     1741 tctatttaat tggtggtgaa atgcgccata ttacagaagc atttgtaggt gaaatggcga
     1801 atgccatgtt agaaaaacta agatttagca agatgttctt tagtagtaac gcagtaaata
     1861 aaggtgctgt gatgacatct acattagatg aagcttatac gcaacaattg gcactaagta
     1921 attcaattga aaaatacttg ctaattgatc atacgaaagt tgggaaagaa gattttacat
     1981 cattttgtca gttaaacgaa ttgactgcag tggtcatgga ctatgaagat gaagaaaaag
     2041 tagaaacgat taaaacatat attgaagtag tcgactaaat taaaaatata actgcattgt
     2101 taaaatatgt gtctatatgc tcataagtga atgtgaattt gcattcattt atgggctatt
     2161 tttttgcaaa tttatgtaaa cgggaacccc gacaaatttt tactacaatt tcagacgaat
     2221 acctaattaa aacaacacca caataagaat gttcacttat tgtttgaaaa tatgtcataa
     2281 acaaacagat tataaacata ttatgttgat ttattgtttg tttatgttta taatttaaac
     2341 gtaatcaaat tgaaagcctt ttctcaagat tttcataacc tacaaataaa tatgtcatat
     2401 tttaagaaag gtatttcaaa attaaagtaa aaaggagtct tattatggcg attattattg
     2461 gttcagatga agctggcaaa cgattaaaag aagtcatcaa atcatactta ttagacaata
     2521 aatatgacgt tgttgacgta acagaaggac aggaagttga ctttgttgat gcaactttgg
     2581 ctgtagcaaa agatgttcaa agtcaagaag gtaacttagg tattgttatc gatgcatttg
     2641 gtgcaggtag ctttatggtt gctacaaaaa tcaaaggcat gattgctgct gaagtatcag
     2701 atgaacgctc tggatatatg actagaggcc ataacaattc aagaatgatc actatgggat
     2761 ctgaaattgt tggagacaca ttagcgaaaa atgtcgttaa aggttttgtt gaaggtaaat
     2821 acgatggtgg tagacaccaa atccgcgtag atatgcttaa taaaatgtgc taaggaggaa
     2881 aaattactat gaagattgca ttaggatgcg accatattgt tacagataca aaaatgcgtg
     2941 tatctgaatt tttaaaatca aaaggacatg aagtcattga cgtaggaaca tacgatttca
     3001 caagaacaca ttatccaatt tttggtaaaa aagttggaga acaagttgtt agcggtaatg
     3061 cagacttagg tgtttgcatt tgtggaacag gtgttggtat taacaatgct gtaaataaag
     3121 tacctggcgt tcgttcagca ctagtacgtg atatgacatc agcgctatat gctaaagaag
     3181 aattaaatgc gaacgttatt ggctttggtg gacgtattat tggtgagtta ttaatgtgcg
     3241 atattatcga tgcatttatt aatgctgaat ataaaccaac tgaagagaac aaaaaattaa
     3301 tcgctaaaat taaacattta gaaacaagca atgcagatca agctgatcca catttctttg
     3361 atgaattctt agaaaaatgg gacagaggcg aataccacga ttaagggacg acacatatga
     3421 ttttaacttt aacattaaat ccatccgttg atatttcata cccattaaca gcattgaaat
     3481 tagatgatgt taatcgtgtt caagaagtaa gtaaaacagc tggtggtaaa ggtttaaatg
     3541 taacaagagt acttgcgcaa gtaggtgagc cagtccttgc gagtggtttt attggcggtg
     3601 aattaggtca atttattgct aaaaaattag atcatgccgg catcaagcat gcattttata
     3661 acattaaggg tgaaacacga aactgcattg caattttaca tgaaggacaa caaacagaaa
     3721 ttttagagca gggaccggag attgataatc aagaggctgc aggatttatt aaacattttg
     3781 agcaattact agaaaaagtt gaagcagttg ctatttcagg tagcttacca aaaggattaa
     3841 atcaagatta ttatgcacaa atcattgaac gatgccaaaa caaaggtgtg cctgtgatat
     3901 tagactgctc gggtgcaact ttacaaacag tattggaaaa tccatacaaa ccaacagtca
     3961 ttaaaccaaa catttctgaa ttatatcagt tgcttaacca gccacttgat gaaagtttag
     4021 aaagtttgaa acaagcagtt tcacaaccct tattcgaagg aattgagtgg attattgtgt
     4081 ctttaggtgc acaaggtgcg ttcgctaagc acaatcacac attctataga gtgaatattc
     4141 cgaaaataag cgtattaaat ccggttggtt caggtgattc tacagttgct ggaattactt
     4201 cagcaattct taatcatgaa aacgaccacg atttactaaa aaaagccaat actttaggca
     4261 tgctgaatgc gcaagaagca caaacaggtt atgtcaattt aaataattat gatgatttat
     4321 tcaatcaaat tgaagtttta gaggtgtaaa aaatgtcaaa atcgaatcaa aaaatcgcat
     4381 caattgaaca attaagtaat aacgaaggta tcatttctgc attagcattt gaccaacgtg
     4441 gtgctttaaa acgcatgatg gcaaaacatc aaactgaaga accaactgta gctcaaattg
     4501 aacaattaaa agtattagtt gcggaagaat taacgcaata tgcatcttca attttattag
     4561 atcctgaata cggtttacca gcttctgatg cacgtaacaa agattgtgga ttattacttg
     4621 cttatgaaaa aactggatat gacgtaaacg ctaaaggtcg tttacctgac tgtttagtag
     4681 agtggtctgc aaaacgttta aaagaacaag gtgcaaatgc tgttaaattc ttactttact
     4741 atgatgttga tgatgctgaa gaaattaaca ttcaaaagaa agcatatatt gaaagaatcg
     4801 gttcagaatg tgttgcagaa gatattccat tctttttaga agtattaaca tacgatgaca
     4861 acattcctga taacggtagt gttgaatttg caaaagttaa gcctagaaag gttaacgaag
     4921 caatgaaatt gttctcagaa ccacgcttta acgttgatgt attgaaagtt gaagtaccag
     4981 taaacatgaa gtatgtagaa ggctttgctg aaggtgaagt ggtttataca aaagaggaag
     5041 cggcacaaca ctttaaagat caagatgcat caactcattt accatacatt tacttaagtg
     5101 ctggtgtttc tgcggaatta ttccaagaaa ctttaaaatt tgctcacgaa gctggtgcta
     5161 aattcaacgg tgtactttgt ggccgtgcta catggtctgg tgctgttcaa gtatacattg
     5221 aacaaggtga agatgctgct cgtgaatggt tacgtacaac tggtttcaaa aatattgatg
     5281 acttaaataa agtattaaaa gatactgcga catcttggaa acaaagaaaa taagtataaa
     5341 aggggattaa tgctatgaat agagaagaag tccaattatt aggttttgaa attgttgcat
     5401 ttgcagggga tgcacgttct aagtttttag aagcattaac agcagctcaa gctggagatt
     5461 ttgcaaaagc agatgcactg attgaagaag gaaacaattg cattgctgaa gcgcatagag
     5521 cacaaacaag cttattagct aaagaagcgc aaggtgatga tattgcatac agtgtgacga
     5581 tgatgcatgg tcaagaccat ttaatgacaa caattttact gaaagattta atgaagcatt
     5641 tattagaatt ttataaaaga gggtgatgac aatgatgcaa aaattgatcg cacaaattga
     5701 aaaagggaag ccatttttcg agaaattatc acgtaacata tatttaagag ccatccgtga
     5761 tggatttatt tctgcaatgc ctgtcatctt attctcaagt atctttctat taattgcata
     5821 cgtgccaaat atatttggtt ttaaatggga taaaggcatg gaagccattt taatgaaacc
     5881 atataactat acgatggggc ttgtagcatt tttagtagca ggaacgacag cgaaatcttt
     5941 aacagattca tttaaccgta agctagaaag tacaaatcaa attaacttta tttctacaat
     6001 gctagcagcg atgtgtggat tcttgttctt agcttctgat ccagccaaag atggtggatt
     6061 cttaagtgcg tttatgggaa ctaaaggttt attaacagca ttcttatcag catttgtaac
     6121 tgttattgtt tataacttct gtgtgaagcg caatattaca attaaaatgc cgaaagaagt
     6181 accgccgaat atttcacaag tatttaagga cttaattcca ttttcagcgg taatcatcat
     6241 tctttatgca ctagatttag tcattcgcaa cagctttaaa tcaaatgtag cggaaggtat
     6301 tttaaaatta ttcgaaccat tatttacagc agcagatgga tggataggtg tcacaattat
     6361 ctttggtgcc tttgcattat tctggtttgt aggaattcat ggtccatcaa tagtagaacc
     6421 agcaattgca gccattacat atgcgaatat cgaagcgaac ttcaagttgc ttcaagctgg
     6481 tgaacacgca gataaaatta ttacatcagg tacacaaatg tttatcgtta catttggtgg
     6541 tacgggtgca acattggtcg tgccatttat gtttatgtgg atgacgaaat ctaaacgaaa
     6601 caaagcgatt ggacgtgcat ctgttgtacc aacattcttt ggtgtaaacg agccgatttt
     6661 attcggggca ccactagtat tgaatcctgt gttctttatt ccatttgtat tagcgccaat
     6721 tgtgaacgta tggatattta aacttttcgt tgaagtgtta ggaatgaaca gttttagtgt
     6781 gaacttacca tggacaacac caggtccact cggcatcatc atgggtactg gatttggttt
     6841 atggtcattc gtactagcga ttactttaat tgttgtagat attattattt actacccatt
     6901 cctaaaagtt tatgatagtg aaattcttga tgaagaagaa ggacgtaaag aaagtaattc
     6961 agatttaaaa gaaaaagttg cagcaaactt tgatacgaaa aaagctgatt caattttagc
     7021 ggcaagtggt gtatcagacg atgcagctaa agcttcaaat atcacagaac aaacaaatgt
     7081 cttagtactt tgtgccggtg gtggtacaag tggattatta gccaacgcac ttaataaagc
     7141 agctgaagaa tatcatgtgc cagtgaaagc agcagctggc ggatatggcg cacatatgga
     7201 cattatgaaa gagtaccaat taatcatttt agcgccacaa gttgcgtcta actatgaaga
     7261 tattaagcaa gatacagata gattaggtat taaattagcg aagactcaag gtgctgaata
     7321 tatcaaattg acacgagatg gccaagcagc ccttgatttc gttcaacaac aatttgaaaa
     7381 ttaagaatag gagtttttca tatgactaaa acattacctg aagattttat ttttggtgga
     7441 gcaacagcag catatcaagc agaaggtgcg acaaatacgg atggtaaagg tcgcgtagca
     7501 tgggatacgt atttagaaga aaactattgg tacactgcag aaccagcgag tgatttttat
     7561 aacagatatc cagtggattt agaattaagt gaaaaattcg gtgttaatgg tatccgtatt
     7621 tcaattgcat ggtctcgtat cttcccaaat ggatatggcg aagttaaccc taaaggtgtt
     7681 gagtattacc ataagttatt tgcggagtgt cataaacgtc atgttgaacc atttgtaaca
     7741 ttgcatcatt ttgatacacc agaagtgtta cacaaagatg gcgattttct aaatcgtaaa
     7801 acgattgact actttgtaga ttatgctgaa tattgtttta aagaattccc agaagtaaaa
     7861 tattggacta cattcaatga aattggacca attggtgatg gccaatattt agtaggtaaa
     7921 ttccctccag gtattaaata tgattttgaa aaagttttcc aatctcatca taatatgatg
     7981 gttgctcatg caagagcagt aaaattattt aaagatggcg gctatcaagg agaaattggt
     8041 gttgtacacg cattgccaac aaaatatccg ttcgatccat caaatcctga agatgttcgt
     8101 gcagcagaat tagaagatat catccataac aaattcatct tagatgcaac atatttaggt
     8161 gagtattctc gtgaaacgat ggaaggtgtg caacatatct tatctgttaa tggcggtaaa
     8221 ttaaacatta cagatgaaga ttatgccatt ttagatgcag ccaaagattt aaacgacttc
     8281 ttaggtatca attactacat gagtgattgg atgagaggtt atgatggaga atctgaaatt
     8341 acgcataatg cgacaggtga taaaggcggt tctaaatacc aacttaaagg tgtcggtcaa
     8401 cgtgaatttg atgtcgacgt acctcgcaca gactgggact ggatgatcta tcctcaaggt
     8461 ttatatgatc aaatcatgcg tgttgttaaa gattatccta actatcataa gatttatatc
     8521 actgaaaatg gtttaggata taaagatgaa tttattgaat ctgaaaaaac agttcatgat
     8581 gatgcacgta ttgattatgt aagacaacat ttaaacgtta ttgcagatgc tattaaagat
     8641 ggtgcaaatg ttaaaggtta ctttatttgg tcattaatgg atgtgttctc ttggtcaaat
     8701 ggttatgaaa aacgatatgg tttattctat gtcgattttg agactcaaga acgctatcct
     8761 aagaaaagtg cttattggta caaagagtta gccgaaacaa aagaaattaa ataataaaag
     8821 atattaaaag gcctatcagt cgtgtgctgg taggtcttta tttataaaaa gagaaaaatt
     8881 aaacatgcat atacatattt ttatattaat caatgggaga cacacgattg aaagttaaat
     8941 aagcaatcaa ttaacaaacg ttaacggaca attttccaaa gtcatcttta aagttaatga
     9001 tgtattcaaa atcaactaaa ggacattttt aatcacatcc tttatactta aataaaagac
     9061 atgaatggga gttaaaagtg tgaagaaaat ttttgtaatt agtacgacat tgttagcggt
     9121 cgctattatt ataggcacta ttattacggt tgtgtttagt caacgacagg cacaaacatt
     9181 taaaatacaa caacagcaat ttgttaaaaa gccaattccg acattgtttt tacatggatt
     9241 tggtggtagt gccaactctg aaaaatttat ggtgaagcaa gcagaaaaaa gaggggtaac
     9301 taaagatatc atcactgctt acgtttcaaa ggatggtgca gtgacattta aagggaaact
     9361 aagtaaagat gcagtgaatc ctattgtgaa aattgaactt gaaaataaca gacaaggcta
     9421 tttggataag aatgctgcgt ggtttaaaaa tgtactgact aagttacaat ctgaatacaa
     9481 tttcgataaa ttcaactttg tcggtcactc aatggggaac ttaacatttg cacaatatat
     9541 gatgacatat ggtaacgaca agtcactgcc acagttaaac aaacaagtga atattgctgg
     9601 gacatttaat ggcgttttaa atatgaatga agatgtcaat gaaattacag ttgataaaga
     9661 tggtaaacca agtcgaatga atcaacctta tcagcaatta cgcgtgctaa aagatattta
     9721 taaaggtaaa ggtatcgaag tgctaaatat ttatggagat ttgaaagatg gtacacattc
     9781 agacggcaga gtgtctaata gttcttcgaa atcattaaaa tatttactgg gaaatagtcc
     9841 gaaaagttat cgagaatcta aatatgaagg tgagtctgca cagcatagtc agcttcatga
     9901 gaatgaaaat gttgctaatg agttgatcga cttcttatgg aaaaaataaa gcaactgcta
     9961 gtttttcggg ggcaaaaaag cttgtaatat tagagttttt caaaaattat ctattgtttg
    10021 agagtttcca taaaatgtct aagtcgtaac attttaaatt tatgattata gcaaatataa
    10081 tgtggttgaa gttacatgaa tgaatcagtg cttaagtcca aatataacaa ctaagaatgt
    10141 atttgcatac agacatcacg aagacacaat tcaaacttaa cgcttaagta acaagtaagt
    10201 aaaagtttca aataaagaaa ggtgattaat gatgcaaaat aaacaaattc tttttaacaa
    10261 aataccagaa ggcatgcctc aagaggatac attcaaaatt gaagacattg acacgcctaa
    10321 acttgagagc gatggtgtac ttgtacaaac gctgtatatt tcagtggatc catatatgag
    10381 aggacgtatg actaaggcag attcgtatgt acagcctttt gaaattggta aaccgattgt
    10441 tagccatgtc gttgcaaaag taattgattc gacactcgca gattataaaa aaggagatgt
    10501 cgttgtaggt atgttgcctt ggcgaatcat taatcatgtc caagcggatc aaattactaa
    10561 agttccaacg acagatgtac cacttgattt atatttaagt gtcttaggaa tgccaggaca
    10621 aacggcatat catggtctgc ttgatattgg acaacctaaa gacggtgata ctgttgtggt
    10681 ttcagctgca tctggcgctg taggttcagt tgtaggtcaa attgctaaga ttaaaggttg
    10741 ccgtgttgta ggtattgcgg gtggcgacaa aaaagtaaat tatttaacag agacactggg
    10801 ctttgacgct ggtattgatt ataagaaaga ggattttgca gaatcgttag cacaagcagt
    10861 accagatggt attgatgttt attttgaaaa tgttggtggt acagttggcg atgaagtatt
    10921 taagcattta aatcgttttg cacgtgtacc agtatgtggc gcaatttcgt catataatca
    10981 tcctgaagca gatatcggac cacgtattca aggtacgctt attaagaagc aagtaatgat
    11041 gagaggcttc ttagtaagtg aatttgctaa tgcttttaaa gaagctagtg aacagctcgc
    11101 aacatgggtt caagaaggta aaattcagtc acaagtaacc attgaagatg gttttgaaaa
    11161 tgcgccacgt gcatttaaaa atttatttac gggcgataac tttggtaaac aagttattaa
    11221 agttacggaa taataaagta tacattttta caaatcaact ttgttaattg accaaaattt
    11281 aataactttc ttatctaaag caaatttgtt cagtaacatg gtagaaataa gtctaaaatg
    11341 taatacgaaa gtcgtctgta attatttata tattcaaatg aaaaaggagt catgatgctg
    11401 cgacataagc acacatggct tctattttgt ataataagaa ataagttcat aaatgaggtg
    11461 aaacgatatg aaaatgatag gatttgaaaa gccctttaaa ttggaagaag gaaatttatt
    11521 taaagtatac gaacaaagaa agccaacgcc agaaaacgat gatatcttag tcaaagtaaa
    11581 tagcattagt gtaaatcctg tagatacaaa acaacgacaa atggaagtca ctcaagcacc
    11641 tcgtgtactt gggtttgatg ctattggtac tgttgaagct atcggaccaa acgttacatt
    11701 atttagtccg ggtgacgttg tattttatgc tggatcgcca aatagacaag gatcaaatgc
    11761 gacatatcaa ttggtttcag aagctatagt tgcaaaagcg ccacacaaca taagcgctaa
    11821 tgaagcggtt agtctaccgc taactggtat tacggcatat gaaacgtttt ttgatacatt
    11881 taaaatttca cataatcctt ctgaaaatat aggtaaatcc gtgttaatca ttaatggcgc
    11941 tgggggtgta ggaagtattg ccactcaaat tgctaaacgg tatggtttaa ctgtcattac
    12001 aacagcatca cgacaagaaa caacagagtg gtgtgaaaag atgggcgcag atatcgtatt
    12061 gaatcataaa gaggatttag tgcgtcaatt taaagaaaaa gaaattccat tagtagatta
    12121 tatattttgc acctataata cagacttgta ttacaatact atgattgaac tgataaaacc
    12181 attagggcat attacaacta tcgtagcttt taatgaagat caagatttaa atgcattaaa
    12241 attaaaaagt ataacattta cacatgaatt tatgtttgcg cgtccaattc atcgaacgcc
    12301 tgatatgatt aaacaacatg agtatttaga agatattacg aaaaatatag aatcaggaca
    12361 ttaccaacca acaacgacac aagtttttga aggtttatca cctgaaaact tatatcaagc
    12421 acatctattg ttggaaaaac agtcaatgat tgggaaatta gtaattaata tatagattag
    12481 taagtaaagt cgccttatgc agaaaatact gtatagggcg acttattttt tgtgtttagc
    12541 gactattgat tctaaagtga aatatggcaa ctttgcaggt atcaattcaa taatgcatta
    12601 gaaaggcatt acttaaatac aataaaatac tttgttgttc aatgataaat aatttgagag
    12661 tattttatat atcattttta aaagatgaag cattattaat tgtgtctata taaaattaca
    12721 aatgattaaa atgttgcagt tgtgggtatg aacttaccat tacacagaaa gggtttgtat
    12781 ttatgaaaaa taaatcagta agtctagtgt tttgggtatc gttagtgatt tgtacgatat
    12841 ttgttgcttt tggtgcaata tttccgaaac agttagaaaa actcacgcaa aacattactt
    12901 catttattgc gctacatttt tcttggtatt atttattgct cgttttagtt attttatttg
    12961 tctgtgtgta tatattattt tcaagatacg catcaattac attaggtgaa gaaggtgaag
    13021 acccagaatt ttcattgcca tcttggtttg ctatgttatt tagtgctggt atgggtatag
    13081 gacttgtgtt ctggacaacg gcagaaccaa tcagtcacgc cttcaaatta acgcctattc
    13141 ataaagctgg cacgcaatcc gctattaatg atgcaatgca gttttcattt ttccattggg
    13201 ggattcatgc atgggcagtc tatggtattg tcgcgcttgt gtttgcatat tttagctttc
    13261 ataaaggata tcctggctta gtcagcgcta cattgacgcc tttattaggt gaaaaagcaa
    13321 tgcgtggccc tttaggtggt gccattgatg tattggcagt catagctaca gtaacaggcg
    13381 ttgctgcaac attaggtttc ggtgcattgc aaataaacga aggtttacat tttttattta
    13441 atgtaccgag taacttcacg atgcaagtaa tcttgattgt cattgcaact atattattta
    13501 cttggtcagc atggtcaggt atcgataaag gtatcaaaac gttgagtaat atcaacatgt
    13561 tacttgcttt tgtagtactt cttggtcttt ttattgttgg gccaacgctt tacattttaa
    13621 atacatttac gaatggtcta ggtaattata ttgcaaattt ctttagtatg agtttacgca
    13681 ttccgtctgg cggtcaaaaa ttccaatggt tacaaaattg gacaatcttt tactgggcat
    13741 ggtggatatc ttgggcacca tttgtaggta tatttattgc acgtgtgtca aaaggacgta
    13801 cgattaaaga atttattcta ggtgtacttt ttgtcccggc gttagtttgc tttatattct
    13861 ttgcggtatt cggcgcgtca gcaatttact tacaagataa tcatattgcc gaaattgcta
    13921 aagctgcgac agaaacagca acttttgcaa cgctacaaca ttacccacta ggttttgtat
    13981 taagcttaat tactttactt gttattatga ttttctttgt tacatcagcg gactcggcaa
    14041 cttatgtttt agggatgttg agttcaagtg gtgatattaa tccaaagtca tttgtcaaag
    14101 tgagttgggg tatcattatg gcattatttg caattattat gatttacaca ggtggtacgc
    14161 aagcgataca aaatttatta atcatcgcag cgttgccatt ttccgtggtc attattgcga
    14221 tgatatggtc gcttttaaaa tctttaagtg aagaaaaacc tagaaatagt aataaggttt
    14281 taattaaaca tcgtgatcca gacgtattag aatatcgttc ttcaaaacat attgacgaaa
    14341 attaattaac aaagaggttt tatttacaaa aactagggaa tatattacta aatggtactt
    14401 catcttatat attaaaaata aatttaaagt tattaattta ttatgtttaa gaaaaaatct
    14461 gatgggtaca tatttaatat gtttttaaat taactttaac taaggaggtg tcgttagggg
    14521 tgaaacgact taaaaacttt atccttggct tattaatagt agtaatcgtg gggttcctgt
    14581 tattcatgta tatccaagat ggacgtataa ctgaatatca agactacttc cttcaatttg
    14641 aatggttcca accattactt atttcacttg ctgcactatt gatattaata ggtcttatat
    14701 tagtatttag tatcttcaaa cctacgcatc gaaaacctgg attatacaaa gacttcgatg
    14761 atggtcatgt ttacgtatct cgtaaagctg ttgaaaaaac tgcatttgat acagttgcaa
    14821 aatatgacca agtaagacaa ccgaatgtag ttgcaaaact atataacaaa aaaaataaat
    14881 cttatatcga cattaaaact gactttttcg taccgaataa tgttcaggta caatctttaa
    14941 ctgaagcaat tcgtgcggat attaaaaaga atgtagaaca ttttacagaa atgccagtac
    15001 gtaagttaga agtaaatgtg agagatcaga aaacttctgg tccacgagtg ttgtaaggga
    15061 ggtatatcat ggctaacaat cataaccaaa acggacaaga ctctacacaa caagtgatta
    15121 attttttaaa agtatttaaa tggagaatcg ttggtttctt agcgtttcta ttaatcgcga
    15181 tattattctt aacgttaggt ttttggaaaa caatacttat catcgtttta tgtttaattg
    15241 gtgtaggtat tgggtatatg aaagaccgta agcaagattt tatgaatttt ttaaatcgat
    15301 ggagttaatt catcaattta gaatttaatt aaaaaaatat aaaaaataac aagtaaaagg
    15361 agaatctatt atgactgtag ataacaataa agcaaaacaa gcatacgaca atcaaactgg
    15421 tgttaacgaa aaagaaagag aagagcgtca aaaacaacaa gaacaaaatc aagagcctca
    15481 attcaaaaac aaattaactt tctctgatga agttgttgaa aaaattgctg gtatcgctgc
    15541 acgtgaagtt aaaggtatct tagacatgaa aggtggctta actgatacat tcactaatgc
    15601 attctcaagt ggcaacaacg ttacacaagg tgtatctgtt gaagttggtg aaaaacaagc
    15661 tgctgtagac ttaaaagtaa ttttagaata tggtgaatca gcacctaaaa tcttccgtaa
    15721 agtaactgaa ttagttaaag aacaagttaa atatattact ggtttagatg tagttgaagt
    15781 taacatgcaa gttgacgatg taatgactca aaaagagtgg aaacaaaaac acgaaaaaaa
    15841 taatgaaaac aataaccaag aaagacaagg tttacaataa gttattcgat aaaaaagctc
    15901 ggcacaccat tttagtgccg agcttttttg tgatttttaa taatttgaat atggaaaatg
    15961 tattattctc ttatttgtat agattgtatt taataagtta atgtaatcct tgagctcacg
    16021 attaatgaaa ttctataatt ttaattattt tctcgataca aagggttgtt aactttaata
    16081 taagtataat gatgtgcttc atcttcaagc cgcattgttg taacacactt ataatctata
    16141 aatggcgcga acatggtatc tttaatttca tttatacgat tctcatttac ttgattagat
    16201 ttgtgtgtcg aatgtacaag ttgatcaaag atgttatcta gtacatcacg aacgatatac
    16261 cacatatgtc tttctaagtt tgaatcattc gatgctttag agattgtaag aattaattcg
    16321 cctaaatggt tttgaacggt agaataaaag gctttgttaa acacagacgt ttttgaatca
    16381 gtaagaattc ttgatttttc atggaaatga gatgtactgt atcccatttc atttagttgt
    16441 gctttatcaa ttcttaaacc ttcaaagtct ctaatataca tcgtgtccaa tagaccatct
    16501 ttccgaaatg ttgcaatagc attttgcaag tgagcctcta atgcaatgcc atatttagta
    16561 actagtggga ttacgagacc aagcaatgct ttactgtaag tttcaatcca cgatttcgcc
    16621 gatgattcaa aatcagacaa tgatgcagct gattgataac gtttaatcaa tgtcacgata
    16681 ggtgattcat tgttaaatgg gtaggttgca actaagcttg aaggaatcat tggtgtgact
    16741 tcttgtggaa tcatttggta tatgtttttt ctaaataatg tacctaattg ttcacttcgt
    16801 tcagtttgat agtctgcttc gtcttgctca ttataaaaat gaataccagc aacttcatca
    16861 ataattgttg atgcatagga cttaaatatg acatctttct ccaaaatatc atttaaaata
    16921 cgtgtcatta gtggaccatt gtgcgtcgtt tgttctgata atgtacgaat ttcacctgta
    16981 atatgaacgt ttgtcgacaa tttgatgtgt ggcgacatag ctgggtattt aggaactaat
    17041 gttctgaaag ataaaccggc ataataatcc aacgtatgtt ttgcttcaat gattaattct
    17101 ttatctactt ctgcttgata atcagaatgt aatacgtcat ctaattgcca tggatgtaca
    17161 atcataatgt gataatcatt aaagttaaag tttggtgtaa attcattttc caattgttta
    17221 attaagtccg gaaatagttg atgaacagtt gtatcataat ctttagacag tgacatagta
    17281 cggcttaatt tactgtgaat cagtactatt ttcaacttaa taggctgatt gaattctgaa
    17341 gaatataaga atgtttgtaa tgcatttaaa cctttacgta atttagcccc gggatgtagc
    17401 ggatgacctt caataacggc ttgctctgaa cgcaagtaac tatcttcgct attttcgata
    17461 atattaaata aaggtgtaga atcatgttgc attgacagtg cttgatagct catcgcaaat
    17521 gtcatattag ttgcactgtt tattaaatct tgctgaaatt gatcactagc agcatttttt
    17581 aaatctggtg cttcaattaa aatacactca agaatttcat ttggatggtg tactcgtgta
    17641 atcatatttg taatgttatc tttaatgtag aaagggcctt caacatcaat tcgatcaaag
    17701 gcgtgttctc cagtgatagg agcatataat gtttgcttag cttgtgggaa ctggatttct
    17761 agtatatgag tcgttgagat atctaacata atcaaatcac gactcaatat tttcttactt
    17821 tgagtgcgcg ctttaactaa gttttcgcga tgcattgatg tgactaatct ctgagtgact
    17881 ttatctcttc cttgtaaaat catctcttta aaaatattag cccaatcgct attatgttgt
    17941 tgtaaaaata aatatgtttc ttgttcttct ttgttaaatt ttaatgtctg ttctttaaaa
    18001 attaagttca agttcataat tcacctctat gaaatatttt acaaaagcaa gatagatttg
    18061 tataatccat attaatgata atgattctta ttatcaacag aatgcgggtg taagttttat
    18121 gacaaaatat ttttttagca gttcttttct actatttcta ggtaattgga ttggacaaat
    18181 agggctaaat tggtttgtac ttaccactta tcataacgca gtttatctgg ggattgtcaa
    18241 tttttgcaga cttgtaccaa tattattact aagtgtgtgg gcaggggcaa ttgccgataa
    18301 atatgataaa gggcgattgc tgagaattac aatttcatca tcatttttag taactgcaat
    18361 tttatgtgtg cttacgtata gtttcactgc aattccaatt agcgtcatta ttatatatgc
    18421 gacattaaga gggattttaa gtgcagttga aacaccttta agacaagcaa tcttaccaga
    18481 tttatcagat aaaatatcta ctacacaagc tgtatcattt cattcattca tcattaatat
    18541 ttgtcgttca atagggcctg ccattgctgg tgtcatatta gcagtctatc atgcgccaac
    18601 aacatttctt gcacagtcaa tttgttattt tatcgcagtt ttattatgct taccattaca
    18661 ttttaaagta actaaaatac ctgaagatgc aacaaggtac atgccgttaa aagtcattat
    18721 agattacttc aaattacata tggaaggtcg acaaatattt ataacatcat tattgattat
    18781 ggcgacaggt ttttcatata cgacactttt accagtttta acaaacaaag tatttccggg
    18841 gaaatctgaa atatttggta tcgctatgac gatgtgtgcc attggtggta ttattgcaac
    18901 gctagtttta cctagagtac ttaaatatat tggtatggta aatatgtatt atttaagttc
    18961 atttttattt ggtattgctt tgttaggtgt ggtatttcac aatattgtca tcatgttcat
    19021 ttgtattaca ttgattgggt tatttagtca atgggcacgt acgacaaatc gcgtttattt
    19081 tcaaaataat gttaaagatt atgaacgtgg taaagtactg agtattatta tgatggatag
    19141 aggtatgatt ccattgggaa gtctgttgat gagtatatgt gcagatgtgt ttggcattgt
    19201 tagaactttt tcaataatgg gaataagtac tatatgcatt acaatggtat tctattttat
    19261 aaatagaaag ttgaagttaa agttggagga aagtaatcat ggtatatctt gaatgggcaa
    19321 aggcagatag aaatattcaa tatcgtgtaa ttaacgccat tattaaagaa cgtatttacc
    19381 ccgagcaaac atttatttca caaaaagaat ctttaattga aattcagtat catatgcatg
    19441 tgttgactat tgaagttgtt agaaaaagtg cattagaacg ctatgagttt acaggtgata
    19501 ttacttattt aaataaaggt gaaacgtcgc taatcatgac gttagaaggg ctactagatg
    19561 tgttgaatca tgactttgat atccctattt cagagcgact acgcgaagag ttaatacaca
    19621 gtcgagatag tttagttgaa acatataagc aaatgtctca cagacaaaca ttaataagtc
    19681 aaagttttaa attttcaaga ttaccacaag atattaactt tttttcatgg ttacaacatg
    19741 taaaagatag tgataagaca gatgatttaa cttattctga gagtttggta ccagaggggc
    19801 atccaacaca ccctttaacc aaaacgaaat tgcccttaac tatggaagaa gtacgagcat
    19861 atgcacctga gtttgaaaaa gaaatccctt tgcaaattat gatgattgaa aaagaccatg
    19921 ttgtgtgcac agctatagat ggtaatgatc aatttattat tgatgaaata attcccgaat
    19981 actacaatca gattcgtgtg tttttgaaga gtttaggttt gaaaagtgaa gactatagag
    20041 cgattttagt acatccttgg caatatgatc atacgatagg gaaatatttt gaagcatgga
    20101 ttgctaaaaa aatattaatt ccaacgccgt ttacaatact ttcaaaagca actttatcat
    20161 tcaggacgat gtctttaatt gataaaccat atcatgttaa gttgcccgtc gatgcacaag
    20221 caacaagtgc cgttagaaca gtctcaactg tgactactgt agatggacca aagttaagtt
    20281 atgctttaca aaacatgttg aatcaatatc caggatttaa agttgctatg gaaccgttcg
    20341 gtgaatatgc aaatgttgat aaagataggg cgcgtcagct tgcttgtatt ataagacaaa
    20401 agcctgaaat tgatggaaaa ggtgcaacag tagttagtgc aagtctagtt aataaaaatc
    20461 caatagatca aaaagttatc gtggatagtt acttagagtg gttaaatcaa ggaattacta
    20521 aagaaagtat tacgacattt attgaacgat actctcaagc attaatcccg cctttaattg
    20581 cttttattca aaattatgga attgcattag aagcacacat gcaaaataca gtagtgaact
    20641 tggggccaca ttttgacatt caatttttag tgagagattt aggtggttct agaattgatt
    20701 tagaaacatt acaacatcgt gtatcagata ttaaaattac aaatgatagt ttaatagctg
    20761 attctataga tgcagtgatt gcaaaattcc aacatgctgt tattcaaaat caaatggcag
    20821 aattaatcca tcattttaat cagtatgatt gtgttgaaga agccgaatta tttaacatag
    20881 tacagcaagt agtagcgcat gccattaacc caacattacc acatgcaaat gagttaaaag
    20941 atattttgtt tggaccgaca attactgtca aagcgttgtt aaatatgaga atggaaaata
    21001 aagtaaagca atatttaaat attgagttag ataatccgat aaaaaaagag gtgtagtact
    21061 acatggcaca cgttaatata aatatatcga agattaaata taacgccaaa gtacttcaaa
    21121 cagtttttca aagtaaaaat attcaattca caccagtaat taagtgcata gctggtgacc
    21181 gtacaattgt agaaagctta aaagcgttag gtatcaatca tgttgcagaa tccagattgg
    21241 ataacataat tagtatggca gatcaggatt taacatatac gttattgcgt acgcctgcca
    21301 aaaaagagat ttcagatatg atagaaaaag ttgatatgag tatccaaaca gaattatcta
    21361 ccatacatca tataaatgaa gtagcagaag ttttaggaaa aaaacataag attttattaa
    21421 tggtagattg gaaagatggc cgtgaaggtg ttctgactta tgatgtttta gattatatta
    21481 aagagattat tcacttgaaa aatatccatt ttgttggttt agcattcaat tttatgtgtt
    21541 ttaaatctga tgcgccaagt gatgatgata tctttatgat taatcgattc gtctcagctg
    21601 ttgaaagaga aataggatat cgattgaaaa ttatttcagg tggtaattcg agcatgttac
    21661 cacaactttt atataatgat ttgggtaaaa taaatgaatt gcgaataggt gaaacgttgt
    21721 ttagaggtgt tgatacaact acaaatcaag cgatagctat gttatatcaa gatgcgatta
    21781 cattggaggc tgagatttta gaaattaaac ctcgagttaa tacacaaacg catgaatcat
    21841 tcatacaagc tattgtagat attgggtatt tagatacaaa agtcgataat atttcaccta
    21901 tggatcagca tatcaatatt ttaggcgctt cgagtgatca tttaatgctt gatttaaatg
    21961 gtcaaggaca ctatcaagta ggagatcata ttagtttttc acttaattat gaagcacttt
    22021 ctcattcgat gtatatgaaa aatttacata aggtatatat tgatgattca aagattgata
    22081 cgctacttca aaattttgat gtgaaatctc ctgctatggt gaaccaatat taatatattt
    22141 atcaataagt ctaagttgac aagttatact acatatcgtt tgcacggttg tatgataatt
    22201 gttcaactta gattttttgt atttaatgat ttatcaaatt aagtgcaaca gttcgtcaca
    22261 taaaactgca ataggtaata tcagctgaat taaagagata acggtcatgc taaatggtgt
    22321 caattgtatt aattcaaaga tgatatagcc atgatacatt atcagtattt tgtctaagga
    22381 aatgtgctaa ttgtagtcat aattattaga ggaaataaat atagtcatac attttagtaa
    22441 tataaaaaga ttgaacgcta cttgacaatg ataattgtta tcaataaaat aataaatgaa
    22501 gttatacata ttaaggagtg gaacgatgag aggtctaaaa acttttagta tattgggatt
    22561 aatagttgcc ttatttttag ttgcagcttg tggtaatacg gataattcaa gtaaaaaaga
    22621 atcatcaact aaagatacta tttcggtaaa agatgaaaat ggtacagtaa aagtacctaa
    22681 agatgcaaaa cgtatcgttg tattagagta ctcatttgca gatgcattag cagcattaga
    22741 cgttaaacca gttggtattg ctgatgatgg taagaaaaaa cgtatcatta aaccagttag
    22801 agaaaaaatt cgggattata cttctgtagg tacacgtaaa cagccaaact tagaggaaat
    22861 tagtaaatta aaaccggatt taattatcgc tgatagcagt agacataaag gtattaataa
    22921 agaattaaac aaaattgcac caacattatc attaaagagt tttgatggtg actacaagca
    22981 aaatatcgat gctttcaaga caattgcgaa agctttagat aaagaaaaag aaggcgaaaa
    23041 acgtcttgct gaacatgata aattaatcaa taagtataaa gatgaaatta aatttgatag
    23101 aaatcaaaaa gtgcttccag cagttgttgc gaaagctggt ttattagcac atccaaacta
    23161 ttcgtatgtt ggacaatttt taaacgaact tggatttaaa aatgcattaa gtgatgatgt
    23221 aacaaaaggt ttaagtaaat acttgaaagg accttactta caattagata ctgaacattt
    23281 agctgactta aatcctgaac gcatgattat tatgacagat aatgctaaaa aagattctgc
    23341 tgaattcaag aagttacaag aagatgcaac atggaaaaaa ttgaatgcag ttaaaaataa
    23401 tcgtgttgat attgttgacc gtgatgtttg ggcaagatct cgtggcttaa tttcttctga
    23461 agaaatggct aaagaacttg ttgaattatc aaaaaaagaa caaaagtaag gtggaagtaa
    23521 atggctataa aagaattaag tagccaatct gccatagatc ataaaagaaa aagacgcaca
    23581 acactcacgt atatggtgag tgtgtgcttt ctttttattt gtgtatattt aaatatggca
    23641 attggttctt caaaaattaa ttttagcgat atcgttcact atgttactgg tcatacggat
    23701 acaaaagcaa cgtttttatt acataatgta cgtatgccaa ggatgattgc agggttattt
    23761 attggcggtg cgttagcggt atctggtttg ttaatgcaag caatgactcg taacccactg
    23821 gcatctccaa agatttttgg tgttagttct ggcgcgtcat ttgtcattgt attagtaacg
    23881 attattattc catcattaga atactactca ctttatttag gtgtcattgg cgcctttatt
    23941 ggtggattaa ctgtctacac actttctggt gctacaaaag gtatgacgcc tattaaatta
    24001 gcgttggctg gtatggccat tcatttattt tttagtagta tgactgaagg gattataatc
    24061 ttaaatgaaa attctaatga acaagtgatg ttttggcttg ttggttcatt atccagtatg
    24121 aagtgggatg agattttaac cattctacca tggcttattg gtgcattaat tgcaacagtc
    24181 tttattggtc gtcagttaac gataatggaa cttggcgatg atattgctaa gggattgggt
    24241 caaaatataa ataaagttag aatcatcatt ggactattag ttattattct tacaggtatg
    24301 tctgtatcaa tagcaggacc aattggcttt gttggtttaa ttgtgccaca tatcgtcaag
    24361 cgctatgtga gtaagaatta tttagtcatg attccattaa catttattat aggtgcagat
    24421 ttattactac tatcagatgt attaagtcgt ttaattacat atccttatga atcaccagta
    24481 gggattgtta catcattcgt aggtgcactt tacttcttat ttataacgat taaaggggtg
    24541 aaaagaatat gatgacaacg aaaattatac gtcgttatag tttaatcaca atccttttaa
    24601 ttgtgagtat attcataagt ttatgtgtag ggtctgtcat gattcacccg attgatgcta
    24661 ttaagggaat ctttacacaa gatgatttta ttttaaatga gtatcgtata cctagaacgt
    24721 tacttggtat tattatcggt agtagcttgg ctatatcagg tgcaattata caagctgtaa
    24781 taagaaaccc gttagcctca cctgatgtta taggtatttc taaaggtgcc agtttagctg
    24841 ctgtgattat tattatgatc tttccaacag caccattgtt tgtattacct ataggttcat
    24901 tcttaggtgc ctttgcagta agtttatttt tatcattttt aatttcaaaa tatgatgtta
    24961 aaggttcaaa gcttgcctta attggtttag caataggcgc aatatgtacg gcgattgtgc
    25021 agttcttatt aatcagaaat ccactggaag caaacaaagc gcttgtttgg ttgacgggta
    25081 gtctatatgg acatgatatg tcgaatattt atactatatt accttggttt ttaataacga
    25141 taccaatcat cattttattg agttatcaat tagatgtatt gaatctaggt gatgctgttg
    25201 caaccgcact cggtttaaaa gttaaaacta ttaaaatgct attgctaatt ttagcagtaa
    25261 tattagcagg atccgcaatt tcagttgtcg gtggtattag ctttttagga ttaattgcac
    25321 ctcatattgc gagacagtta ataggaaata aaacattaca cgttattatc atgtcggggt
    25381 taattggtgc tatattgtta acatttggtg atggtttagc ccgtggcatt catccgccac
    25441 tggatattcc tgtaggggtt atcattgcaa ttatcggtgc accatacttc ttaatattat
    25501 tacgaagaat gaaataacta acatatgatt agagtttata aaaggttaag tacgaaatca
    25561 gtaattgtac ttaacctttt ttgaattcat cttgaaaaat tacaagaaat agcgtatatt
    25621 taataactgt aattattcat tattttgcat acataaacat tttaaaagat ataatgataa
    25681 ggaatatgtt tgttaactga atagaattct gtatatactt aaaacgaata catagagaaa
    25741 tggattgatt ttatggatag cttttcaagg aaagagatag tcattgggag acttaagttt
    25801 attacgatgt ctttaattgg tatcttatta tttctagtac caattccagt cgaacaagat
    25861 ggacagaagc aaacaacgtt gccagtggca tttttagcag gcgttcttaa agatgtatta
    25921 ggtggcgtaa tgccattttt aattgtgacg ataattactt tgtcgggtat cataacactg
    25981 atttgttcaa ctattttaaa agataaattg aaacctgatg gattattgca taatgcgttt
    26041 aatgtaagaa taggttggtt aattttgcga ttacttgccg ttgtctttgc gtggatgaca
    26101 tttttacgaa ttggttcaaa agtaatatat tcagatgaaa cgggtggatt attgttctcg
    26161 agtttattgc caactttagt ggcagtattc ttatttgcgg cattattttt gccactatta
    26221 atggaatacg gtttgttaga gatgttgggt cctattttta gaccggttat gagaccttta
    26281 tttacattgc caggacgttc gacggtagat aatttagcat catttattgg agacggaact
    26341 gttggggtct taattacaag tagacaatat ggtgaaggat attattctcg tcgtgaggca
    26401 acagttattt caacgacatt tagtgtagtt tctatcacct ttgcaattgt agttgctgaa
    26461 acagtacata tgcaaaatca attttttgct ttctatttat cagtgatagt atcctgcctc
    26521 gttgcagcag tcattatgcc aagaatttgg cctttaaata aaattcctga tgagtatgct
    26581 aaggaggtac ctgaaagtgc gcgtactgag gcattgccag aaggaaagac ggcattgaga
    26641 catggctttg atactgcaac agaagtaggt attaaagcac cgggcattgt tgacttcttt
    26701 aagtcaggat tgaaaacggt tgttgatatg tggtttgtta ttttacctgt tgtgatgagt
    26761 attggtacta ttgctaccat tattgcgaac tacacgccat tgttcgttat tttgggtaaa
    26821 ccttttgttc catttttaga gttaatgcat atacctgaag cggcacaggc ttctcaaacg
    26881 attattatcg gttttgccga tatgttttta ccttctatat taattgaagg tgtacaaaat
    26941 gatataactc gttttgttat tggggcactt agtatatcgc agttaatata cttgtctgaa
    27001 gtaggtggcg tcatacttgg ttcgaagatt cctgtaagca tcggcaaatt gtttatgatt
    27061 ttcttgatta gaacaatcat tacactacca attatttctt tgatggcaca cttgttattg
    27121 taattagata tagaaaagga caactgttaa aattgtgaaa acagttgtcc ttttgtattt
    27181 gtttatgctg ggtgttgaat caattaaaaa aatcattcat gaagccctca ccatgaatga
    27241 tttttttata caatttattt agttaattca ttgtaaactt tttgatcatt ttgagtataa
    27301 ataatgtatt cgtggtcttc agtatcgata ataacgcgat tcgttttacc aaatgttgag
    27361 ccgattctag atacatcttt gctgtctaat ttaggaactg cgtgaatatc ttggtcttgt
    27421 gtaatatttt taatttcact tgtaggaatt ttgatatcag caactctcca ttgaatacgc
    27481 acttcattat tatccttttt tactgtcatt gccattattc aaacacatcc tttaaatatt
    27541 ttgtaaatac atcataaacg attttgtaat cgttttcaag agttatgtga tactttttta
    27601 aattgtgtca tactaatgaa gttcaacccg aaaatatgtt cgtgtttttg gcgaatttat
    27661 ggtaaaatat gagtattaaa ggagaacaaa tatgacaggt aaaacacatg cttcatgcgg
    27721 catgctcgtt ggagcgctaa caacacaata ttttcaaacg gatatttttt cgtcggttac
    27781 ggttatcatt ctagcaactc tagctagctt attacccgat atatgtcaca ctcaaagtaa
    27841 gattggcaga aggtttaagg taattagttt ttttgtgaga ttgatctttg gacatcgaac
    27901 ctttacgcat tcaatattat ttatcgcaat catagcattt ttattgcaaa ttattcagac
    27961 gcctaattat tatatggcag caattataat tggattagtg tcacatgtta tactagatat
    28021 gataacacca aaaggcgtga aattatttta tccgttaccg tttaatgtga agacaccgat
    28081 tcaatttaaa acaggtggac ttgtagactt atctttagct actgcgttaa tgatcggaac
    28141 agtttatgtt ttatttcagc ctttcatcaa tgatataata agcaactgga atactaagtt
    28201 tttatagaaa ataaattatt aaaggttaca aactcagcag gaggcataaa atgctagata
    28261 aaaatcaatt agctaaatat aaacaagatc atttatgtga atatgaaaaa ataatgagta
    28321 acaatgaaaa agaagcactt gaagagaagg tagcgtcatt agatttagat tttattgcca
    28381 aattatataa cgatttatac atcaataaga aaacgataga tgatgtatct gcagtttctg
    28441 aagtgaaata tgatattaaa tcacaaatga gtgatgatga gattaagcgt ttagaggaac
    28501 aaggacttca agctattaaa gaaggacaat ttgcagtact tttgatggca ggtggacaag
    28561 gaacaagact tggatataaa ggacctaaag gttcattcga aattgaaggt gtaagtttat
    28621 ttgaacttca ggcaaaacaa ttaaaagaac tacatcgaca aactggtcat aaaattcaat
    28681 ggtacattat gacaagtgat atcaatcatg aagaaacttt agcttatttt gaatcacata
    28741 actactttgg ttatgatcaa gaatcaatcc atttctttaa gcaagataat attgtggcgc
    28801 ttagtgaagc aggacaatta attttaaatc aacagggtcg tattatggaa acgccgaatg
    28861 gtaatggtgg cgtgtttaaa tctttagaca aggcaggata cttagaagaa atgtctaata
    28921 atggcgttaa gtatattttc ttgaataata tcgataatgt tttagtaaaa gtgttggatc
    28981 cactatttgc aggatttacg gtagaacatg attacgatat tacttctaaa acgattcaac
    29041 caaaaccagg agaaagtgtt gggcgattag ttaatgtcga ttgtaaagat acagtattgg
    29101 agtattcaga attagatcca gaagttgcaa atcaatttaa caatgctaat atcggtattc
    29161 atgcatttaa acttggtttt attttaaatg ctgtaaatcg tgaattacct tatcatttag
    29221 ctattaagaa tttgaagcaa ttagatgaga actttggtgt tattgaacaa ccaactttaa
    29281 aattcgaatt gttctatttt gacatattta cttatggaac tagttttgtc acgttacaag
    29341 ttcctagaga agaagagttc tcacctctta aaaataaaga aggtaaagat agtgttgcaa
    29401 cagcgactga agatttacgt agaatgggtt taatttaatg ttaggcggtg acttacgtgt
    29461 ccaaatctag tgaaaaaagt aatgacacaa tcgtagaaac atttaaggat atcatccctt
    29521 tatcatttgg agaagaaatt ggtaatgcag catctcatgg tgttgcagct ttgttaacat
    29581 tattagtgtt accctatgca gctgtgcaca gttatattgc atatggtacg cttgcgtctg
    29641 tgagtatgtc tatttatgtt atttctatct ttatgatgtt tatttcatct actatttatc
    29701 attctatgca aaatgaaacg cctcataaat atattttaag aattattgat catagtatga
    29761 tttatgtagc aatttcaggt acatatacgc ctattttatt aacagttgta ggtggttgga
    29821 ttggatggac agtgtttatc ctattatggg gcacaacgct ttggggaatt ttatataaat
    29881 ccatagctgt caaagttaac caaaagttaa gcttaattgt ctatttagtg atgggctggg
    29941 taggtattat cttgttacca attattttta ttcgcacatc atggatattc atattattca
    30001 ttttcttagg cggtgtgtca tatacgattg gtgcatggtt ctatgcacaa aaaaacagac
    30061 catattttca tatgatatgg catattttta ttgttatagc atcattattg catttaattg
    30121 ctattttata ttttatgtaa tagtttatca gaaggtgggg ggaactccgc tttttttatt
    30181 acttaattca ttctaataga ggattgtttt gtatgactat tatcgttaag atttgaaagt
    30241 caaaagtgtt tatacaaata caaatcaata gtttgtaaat aaaatcgtat aaatatatat
    30301 aaagaaggag gtatagcatg cgtttaaaat caattgtaac tgtaatagct ttaatattaa
    30361 tcatgtttat ggctgcaatc gaatcatcaa ttatttcttt agcgttacca acaattaagc
    30421 aagatttaaa tgcagggaat ttaatttcat taatttttac cgcgtatttt atcgcattag
    30481 taattgccaa tcctatagta ggtgaattat taactcgttt taaaattatt tatattgcta
    30541 tcgcaggtct tacactattc acggttggta gtttaatgtc aggtttaagc acacatttta
    30601 gtatgttggt tatatcacgt gtaattcaag ggtttggatc tggtgtcatg atgtccttgt
    30661 cacaaattgt gcccaaatta gcatttgaaa ttccattacg ctataaaatt atgggtatcg
    30721 ttggtagcgt ttgggggatt tcgagtatca ttggaccact tctaggtggt ggcatattag
    30781 aatttgcaac atggcattgg ttattctaca taaacatacc gattgcgatt attgcaataa
    30841 tattggttgt ctggacattt cattttccag aagaagaaac tgtggctaaa tcaaaattcg
    30901 atactaaagg tatcaccctg ttttatattt tcattggttt aattatgttt gcattgttaa
    30961 accaacaaca tttatattta aatatcatag gttttgtctt agcaatatta gttgcacttc
    31021 gattatttaa tgttgagaaa aaagtttcat ccccattctt accagtagca gaatttaatc
    31081 gaatgattac tttggtcttt attacagatc ttcttacagc tgtttgttta atgggcttta
    31141 atttatatat tcctgtgtat cttcaagaac aactggggtt atcgccattg caaagtggat
    31201 tagttatttt tccactttct gtagcgtgga ttacattgaa ttttaatttg caccatattg
    31261 aagcgaagtt atcaagaaaa gttatctatt tgtcgtcttt tacattatta ttattaagta
    31321 gtataatcat tgcgtttggt ataaaattac caattcttat agcatgtgtc ttaattttaa
    31381 gtggactaag ctttggatat atttatacaa aagatagtgt aattgtccaa gaggaaacaa
    31441 gtccaataca aatgaagaaa atgatgtcgt tttatggatt aacaaaaaac cttggcgcat
    31501 caataggttc gacgattatg ggataccttt atgcgttaaa atcaggattg tttggtgcaa
    31561 acttgcataa tattttaggc gttgtatcct tgataagtgt agggctcatc gttgtatggc
    31621 tcatatttta tagagaagcg gcgtcacaaa caaaagaata gaattatgat taaaattgat
    31681 gtagtgtaaa taaagtaaca aataatcatt ttactaagtt tgaagtgtaa tcaactatct
    31741 taaaattaaa agcaatataa attatttatg gtaaggagga caatagttat gaattatctt
    31801 aaacataagt tttataattt attaacaaca attattgttc tctttatctt tgtgctttct
    31861 ggtgcgatat tcttaacgtt tttagggttc ggactatatg gattaagtag aattcttatt
    31921 tattttaggt taggtgactt cacatataac agaagtatgt acgacaattt gctatattat
    31981 ggtagttata ttatatttgg ctactttatc gtatttgcag ttgaacattt gatggattat
    32041 tttagaaaaa tgttacctga aaatgcttat tttagaggtg caacttttca tttaatctct
    32101 tatatggttg caacaacgtt attttatttt attattcatc tgaactatgt gtatatcaac
    32161 attgattttt gggtcatcat ggttattata ggctttttat atgtttgtaa gctgcagttt
    32221 taccctgaaa gtaaaaatct aaataataga aaatagagtt ttgaagttgg gggcaatgat
    32281 gtgttccaac ttctttttat tcatatagta gaaaatgagt tgttttgctt caattaatca
    32341 cggttaataa taaccattga tataaatagt tggtacttta atctgaaata tattttaagt
    32401 atatatgcta ataaacgtaa atttgttaca aaggaggaat tatgatggct aaagttgaat
    32461 taacaacccg gagacgaaac ttcatagttg ctgttatgtt gattagtgcg tttgtagcta
    32521 ttttaaatca aacattatta aatacagcgt tacctagtat aatgagagaa ttaaatatta
    32581 atgaaagtac atcgcaatgg ctagttactg ggtttatgct tgttaatggc gtcatgatac
    32641 ctctaacagc atacttaatg gacaggatta aaactagacc tttatactta gcagcgatgg
    32701 ggacattttt agtaggatca attgttgcag ccatggcacc caattttgga gttttaatgt
    32761 tagcacgtgt catccaagct atggggggca ggcgtactca tgcctttaat gcaattcaca
    32821 ttgtttacgt tattcagtaa agaacataga ggatttgcaa tgggattagc tggtttagtt
    32881 attcaatttg caccagcaat cgggcctact gttactggat taatcattga tcaagcgagt
    32941 tggcgagtac catttattat aattgtagga attgctttag tcgcattcat cttcggttta
    33001 atttcaattt caagctataa tgaagtgaaa tatactaagc tagataagcg ctctgtaatg
    33061 tattcaacga ttggatttgg attaatgtta tatgcattca gtagtgcagg cgacctagga
    33121 tttactagcc cggtagtcat tggtgcaatg ataattagtt ctattatcat ctacttattt
    33181 attcgtagac aattcaatat aagcaatgca cttttaaatt taagtgtttt taagaataga
    33241 acatttgcat tatgtacgat tagttcaatg attataatga tgtcaatggt tggtccagcg
    33301 ttattaatac cgctctatgt tcaaaatagc ttagctctat ctgcattatt gtttggactt
    33361 gttatcatgc caggtgcaat cataaatggt attatgtcgg tttttacagg taaattttat
    33421 gataagtatg gtcctatacc actgatttat actggtttta cgattttaac aattactact
    33481 attatgttgt gtttcttgca tacagacaca tcttatacgt atttaatagt cgtatatgca
    33541 attagaatgt tttcagtttc tttactcatg atgccgataa atactacagg aattaattct
    33601 ttgaaaaatg aagaaatctc acatggcacg gctattatga actttggtcg tgtaatggct
    33661 ggctcattag gaacagcatt aatggttact ttgatgagtt ttggcgcaaa aatattctca
    33721 tctacatcgc catcgcattt aactgcaact gaaattaaac agcaatcaat tgctataggg
    33781 gtggatatct catttgcttt tgtagctgtg cttgttatgg cagcttatgt gatagcgctt
    33841 tttataagag aacctaaaga aatagaaagt aatagaagga aattttaaaa taattatagt
    33901 agttggtcta ttgaaaataa taggctaact tcttttttta tttaatgaaa agttttatac
    33961 ttttattgat agactaagca aaaattgtta tttgctatga tgtagatatt ttaaattgat
    34021 taagggggat ttgctttgtt aacggtagat caagttaaag aactaatagg agaaattaaa
    34081 gatcctatta tagatgtacc tttaaaagaa acagaaggta ttgtagaagt ttctattaag
    34141 gaagaaaaag aacatgtaag tgttaaagtg gcaatggcac aattaggtgg tgcaccgcaa
    34201 ttagatttac agatggcagt tgttaatgtt ttaaaagaaa atggtgcaaa aactgtgggt
    34261 atacgatttg aaacgttacc agaagaaaaa gtaaatcaat ttaaaccaaa agaagaaaat
    34321 aaacctaaaa cgatagaagg cttattatct caaaataatc cagttgaatt tattgctatt
    34381 gcttcaggta aaggtggcgt cggtaaatct actgttgcag taaatttagc cgttgcctta
    34441 gcccgtgaag gtaaaaaagt tggattagta gatgccgata tatatggatt tagtgtacca
    34501 gatatgatgg gtattgatga aaagcctgga attaatggga aggaagtaat tccagttgaa
    34561 cgtcatggcg ttaaagttat atcaatggca ttttttgtgg aagaaaatgc accagttata
    34621 tggagaggac caatgttagg taaaatgttg acgaatttct ttacagaagt tagatgggga
    34681 gacattgaat atttaatact cgatcttcca cctggaacag gagatgtagc tttagatgtt
    34741 catacgatgt taccttcaag taaggaaatt attgtaacga cacctcatcc tacagcagca
    34801 tttgttgcag ctcgcgcagg tgcgatggca aaacatacgg atcattctat tcttggcgta
    34861 attgaaaaca tgagttattt tgaaagtaaa gagacgggga ataaagaata tgtctttggc
    34921 aaaggtggcg gtactaagtt agctgatgaa cttaatactc aattacttgg ggaattacct
    34981 ttagagcaac catcttggaa tccaaaagat tttgcaccgt caatatatca atcagatgat
    35041 cgtctaggta aaatttatag ctcgattgca caaaaagtta tagctgccac aaataaatag
    35101 gtataatata tgctaaacgt acaaaattac ttttattttg tacgttttta tattgatttg
    35161 ttttggaatg ctggtaaaat aagttcttgt gttaagcaat aaacgcattg aaaaaagaag
    35221 cgacttgcaa acacgaatgt ttttcaaaaa agttattgac tttaaattat aaagttgata
    35281 agataataaa gtcgtcaaaa acggtgatga tgtttttaaa gaagaatttc aaaagtgtaa
    35341 aacttaccat tgaaataact ataaaaacaa tataaaataa ataaagtaat cgttcgaaaa
    35401 gttattaact tcaataatta aatgaagtac aataaaagag attgaac
//
LOCUS       DAKSQE010000015        35390 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.015, whole genome shotgun sequence.
ACCESSION   DAKSQE010000015 DAKSQE010000000
VERSION     DAKSQE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 35390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35390
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.015"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            41..493
                     /gene="argR"
                     /locus_tag="O0V33_000602"
     CDS             41..493
                     /gene="argR"
                     /locus_tag="O0V33_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCX8975272.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
     gene            509..2188
                     /gene="recN"
                     /locus_tag="O0V33_000603"
     CDS             509..2188
                     /gene="recN"
                     /locus_tag="O0V33_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCX8975273.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSG
                     KSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
                     KYHQTFQNYKAKKQGLEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIK
                     RIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFY
                     YILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNE
                     INKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKD
                     ANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVK
                     SRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISK
                     SSKDDRTTTQVQELIGDDKVDEIARMISGASVTDLTRENAREMIQHNQRHR"
     gene            2339..3760
                     /gene="lpdA"
                     /locus_tag="O0V33_000604"
     CDS             2339..3760
                     /gene="lpdA"
                     /locus_tag="O0V33_000604"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCX8975274.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSVGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDANEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            3776..4768
                     /locus_tag="O0V33_000605"
     CDS             3776..4768
                     /locus_tag="O0V33_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HCX8975275.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGLHGEQVDGNDPLA
                     MYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEEREALKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            4768..5751
                     /locus_tag="O0V33_000606"
     CDS             4768..5751
                     /locus_tag="O0V33_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCX8975276.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGKKGGVFGTTQGL
                     QQQYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            5764..7038
                     /locus_tag="O0V33_000607"
     CDS             5764..7038
                     /locus_tag="O0V33_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCX8975277.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASSTVEQTSTSKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDLMSVI
                     ENGGTTAQSDKQVQTQSTSVDRSSNQSSEDDSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNNFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTTEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIVKKPVVINDMIAIRSMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            7388..7825
                     /locus_tag="O0V33_000608"
     CDS             7388..7825
                     /locus_tag="O0V33_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCX8975278.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGNITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            7839..8819
                     /locus_tag="O0V33_000609"
     CDS             7839..8819
                     /locus_tag="O0V33_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCX8975279.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIILLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEALLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVASLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(8946..9125)
                     /gene="prli42"
                     /locus_tag="O0V33_000610"
     CDS             complement(8946..9125)
                     /gene="prli42"
                     /locus_tag="O0V33_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HCX8975280.1"
                     /translation="MLVISSQVKNFISYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
     gene            9388..10521
                     /locus_tag="O0V33_000611"
     CDS             9388..10521
                     /locus_tag="O0V33_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCX8975281.1"
                     /translation="MINEQRLLNTFLELVQIDSETGNESTIQPILKEKFISLGLDVKE
                     DEAAKHPKLGANNLVCTMKSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIFGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAVVTVEQSYPGFKINDNETVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARQSK"
     gene            10589..11995
                     /gene="gndA"
                     /locus_tag="O0V33_000612"
     CDS             10589..11995
                     /gene="gndA"
                     /locus_tag="O0V33_000612"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCX8975282.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            12311..13524
                     /locus_tag="O0V33_000613"
                     /pseudo
     CDS             12311..13524
                     /locus_tag="O0V33_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13539..14438
                     /locus_tag="O0V33_000614"
     CDS             13539..14438
                     /locus_tag="O0V33_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCX8975283.1"
                     /translation="MKNSQNIVCIGAANIDKQLYLKSELVNKSSNPVSSISVSGGVAR
                     NLAYNIGKLSNKKTILLTLLGNDKEADYILNEANKFIDLKHIKIIENMNTGMFTSVID
                     YNGEMICGFADMDIYNLINNKYLNEKIEVIKNADCVVVDSNIPKESIQYLIQLCGKSN
                     IPIGIIPASIKKVRNILKNLAKIDYLIFNKKEAEAFWNVSLNTKSDYKKIKKISKTSE
                     IKNIVITNGNDDIFYTNGELSIFKNVQQSSNIINDTGAGDVFSASVFHYWLENYEVDN
                     YLEMALFNACSTVSTIYNTSMIN"
     gene            complement(14510..14671)
                     /locus_tag="O0V33_000615"
                     /pseudo
     CDS             complement(14510..14671)
                     /locus_tag="O0V33_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            14915..15286
                     /locus_tag="O0V33_000616"
     CDS             14915..15286
                     /locus_tag="O0V33_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCX8975284.1"
                     /translation="MEKLNQVMLYVDDVEKAKAFWTEILEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKYSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MNGQRVFNFQDGQGNYFAVSD"
     gene            15225..15479
                     /locus_tag="O0V33_000617"
     CDS             15225..15479
                     /locus_tag="O0V33_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975285.1"
                     /translation="MVNVYLISKMAKVIILQLVTKQSILIFIQTFILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFLESVTKLVLYKGWKRFPVQRLTGGD"
     gene            15480..16499
                     /locus_tag="O0V33_000618"
     CDS             15480..16499
                     /locus_tag="O0V33_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCX8975286.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGDFEVSKDRIQGFETVASQFNLDYQI
                     IETSNEREVIFNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTSIDIKPRMLGQQAGAAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
     gene            16515..18164
                     /locus_tag="O0V33_000619"
     CDS             16515..18164
                     /locus_tag="O0V33_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCX8975287.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDLG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDRLLKGVHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQL
                     EDEDSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDISHGEVKLFNYDAKVNLKQLLPYEACVIELN"
     gene            complement(18247..19113)
                     /locus_tag="O0V33_000620"
     CDS             complement(18247..19113)
                     /locus_tag="O0V33_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCX8975288.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(19343..20827)
                     /locus_tag="O0V33_000621"
     CDS             complement(19343..20827)
                     /locus_tag="O0V33_000621"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCX8975289.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
     gene            21140..22060
                     /gene="rnz"
                     /locus_tag="O0V33_000622"
     CDS             21140..22060
                     /gene="rnz"
                     /locus_tag="O0V33_000622"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCX8975290.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPIVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQTLIQNEDTPNFNFVKDFDSFKI"
     gene            complement(22165..22980)
                     /gene="proC"
                     /locus_tag="O0V33_000623"
     CDS             complement(22165..22980)
                     /gene="proC"
                     /locus_tag="O0V33_000623"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCX8975291.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNIEDQ"
     gene            23122..23877
                     /locus_tag="O0V33_000624"
     CDS             23122..23877
                     /locus_tag="O0V33_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCX8975292.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLENKVHVTLLVRDVDKATRIF
                     DQELGKTINVIPCDLNDLKSIQALQFEDNTLFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKKMGKIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
     gene            23893..24141
                     /locus_tag="O0V33_000625"
     CDS             23893..24141
                     /locus_tag="O0V33_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975293.1"
                     /translation="MNCQSESVVRLCVRYAEQLSVFEEFTVLDILSDISVDQISDSTL
                     YYTCEKFKLLVLQGNVLGVQVITNNDELTCEVKYRKMF"
     gene            complement(24357..25265)
                     /locus_tag="O0V33_000626"
     CDS             complement(24357..25265)
                     /locus_tag="O0V33_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCX8975294.1"
                     /translation="MQKNILKSGIALSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALFKYQQRDDIVIGTKVGNRLTKDGSTTWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNDDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVSLDQIKTARARVKDLEYTNHLV"
     gene            25347..25889
                     /locus_tag="O0V33_000627"
     CDS             25347..25889
                     /locus_tag="O0V33_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCX8975295.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCALTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDEDRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNVEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            25994..26443
                     /locus_tag="O0V33_000628"
     CDS             25994..26443
                     /locus_tag="O0V33_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCX8975296.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            26492..27379
                     /gene="xerD"
                     /locus_tag="O0V33_000629"
     CDS             26492..27379
                     /gene="xerD"
                     /locus_tag="O0V33_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCX8975297.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLVLLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(27457..27963)
                     /locus_tag="O0V33_000630"
     CDS             complement(27457..27963)
                     /locus_tag="O0V33_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HCX8975298.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEKQIAKLNA
                     AKPFSSVILPITPDFERIIKANYPDYDYNHETATDPFIVDHHMRRDRSEVIAAKEEAI
                     QLRKHRRN"
     gene            28055..28786
                     /locus_tag="O0V33_000631"
     CDS             28055..28786
                     /locus_tag="O0V33_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCX8975299.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDIDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTELSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIVRGVNYHFG"
     gene            28779..29321
                     /gene="scpB"
                     /locus_tag="O0V33_000632"
     CDS             28779..29321
                     /gene="scpB"
                     /locus_tag="O0V33_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCX8975300.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDISKDQLVELIENYSS
                     HGLIIQRFGTTYVLTTKKEASMYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            29314..30051
                     /locus_tag="O0V33_000633"
     CDS             29314..30051
                     /locus_tag="O0V33_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCX8975301.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            30184..30909
                     /gene="srrA"
                     /locus_tag="O0V33_000634"
     CDS             30184..30909
                     /gene="srrA"
                     /locus_tag="O0V33_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HCX8975302.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            30890..32641
                     /gene="srrB"
                     /locus_tag="O0V33_000635"
     CDS             30890..32641
                     /gene="srrB"
                     /locus_tag="O0V33_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HCX8975303.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNTRFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWTYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
     gene            32893..33825
                     /locus_tag="O0V33_000636"
     CDS             32893..33825
                     /locus_tag="O0V33_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001803819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCX8975304.1"
                     /translation="MKKFIGSVLATTLILGGCSTMENESSKDTNTETKSVPEEMEASK
                     YVGQGFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGDGVEVFVH
                     CDDHDIVFNASIPFDKSIIESDSSLRSEDKGDDMSTLVGTVLSGFEYRAHKQELDSLT
                     EVLKEYKSKYKYTGYTENAIMKTQNSGFRNEYYYLTAIPYTLDEYKKYFQPLIKEDDK
                     TFSDGMKNSKKQLKDKSRPYVVTTLFSTKDNYTKDNTIDEMIDFSEVLKKKKNIPHDL
                     NVSLQITDKYINSARPSYSKKDSIEVGVFNHEKN"
     gene            33812..>35390
                     /locus_tag="O0V33_000637"
     CDS             33812..>35390
                     /locus_tag="O0V33_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975305.1"
                     /translation="MKKINDRDLAELSGYWAYQDLDKRYVIRVNNNKYRLIEDYSDNP
                     NSKSFGGTDAKVFELVNSNNELTGQQAIIFQGTNNNKSIGSNNPFRGKVADDWIENIK
                     LMNDKNKSTSLLEQNNAYIKSYEQKLKDADTLKSSDFLRKYKKNPSTYKNKTIKSDGG
                     NSQGGASANHQGMINPNKNIVSTNPAMLPKSIWENFKSQNFENMINYHSKFDILSWLQ
                     DPFATPTLGKRVNLETGVPTMDGLVNSHLGYKRKLNPRYNTYKDLAVYKIKSVKDTMI
                     KNGKKVKKTIEIDINMDDRIPINVWTGDSIARTGKGTPIKLNLENLSALSNLVTGETS
                     NMLTDCVNYLNESYNISQVENANFGERKHKLKQDFKKIVEVDILEGMSRELTSLKKDA
                     FSAIDNVKKNLVGIAFIFSSVAVLIFELNTIEEMLEDLDKKLQRGIESLHDSLDNVIK
                     EMFKNLDHDFEDGVTEEMMKHLNVVNNNINFVKKQNDVYGEQITDIKNIMSHQDATVM
                     DGNLSINYSGEHMISGQV"
BASE COUNT        12762 a         4869 c         6493 g        11266 t
ORIGIN      
        1 aaaataaatg tataaatatt cgtaatcaga ggtgtttact gtgcccaaaa aatcggttag
       61 gcatataaaa attagagaaa ttatttcaaa tgaacagata gagacacaag atgaattagt
      121 taaacgatta aacgattatg atttaaatgt cactcaagca actgtttctc gtgatattaa
      181 agaactacaa cttattaaag tacctatacc ttcaggtcaa tatgtttata gtttaccaaa
      241 tgatagaaaa ttccatcctt tagaaaaatt gggacgttat ttaatggatt cctttgttaa
      301 tatagatggt actgataatt tacttgttct aaaaacatta cctggtaatg cacaatctat
      361 tggagctata ttagatcaaa tcaattggga agaagtacta ggcacaattt gtggtgatga
      421 tacttgttta attatttgtc gaagcaaaga ggcaagtgat gaaatcaagt caagaatttt
      481 caatttgtta taaggatgcg atgagtttat gttacaaacc ttatcaatca agcaatttgc
      541 tattattgaa gagttagaaa ttcaattttc tgatggttta actgttttaa gtggtgaaac
      601 gggttcaggt aagtcaatta ttatagatgc aattggacaa ttaattggaa tgagagcttc
      661 atctgatttt gtaagacatg gtgaaaaaaa ggctgtcatc gaaggtatat ttgatattga
      721 cgagagtaaa gatgctatac atatattgaa aaatatggat attgatgtag atgaagattt
      781 tttattagtt aaacgggaaa ttttcagctc tggtaaaagt ctttgtaaaa ttaataatca
      841 aacagttact ttacaagatt tacgaaaagt tatgcaagaa ttattagata ttcatggcca
      901 acatgaaacg caatctttgt tgaaacaaaa ataccattta acattattgg ataattacgc
      961 agaatcacgt tatcaagatt tgttggataa atatcaccaa acttttcaaa attataaagc
     1021 caaaaagcaa gggttagaag atttagaatc agcagaccaa gcattgctgc agcgtttaga
     1081 tttaatgaaa ttccaattag aagaattgtc tgaagcacat ctaaaagaag gcgaaataga
     1141 gcaacttgaa atagatatta aacgcattca aaactctgaa aaattaagct tagctcttaa
     1201 taacgcacat atgacattaa cggatgaaaa tgctatcact gatcgtttgt atgaactaag
     1261 caaccatttg ttaacaataa acgatatagt tccaaacaaa tatgacaaat taaaagaaga
     1321 tattgatcaa ttttattaca ttttagaaga tgcaaaacat gagttatatg atgaaatggc
     1381 taatacagaa ttcgacgaac aagtgttaaa tgagtatgaa tctcgtatga atttgctgaa
     1441 taatttaaaa cgcaaatatg gaaaagatat ttcagaatta atcgcatatc aagaaaaact
     1501 taataatgaa atcaataaaa ttgaaaacta tgaacaaagt acatcgcagt tacgagaaga
     1561 aataaatgca ttgtataatc aagttataga ggttggacaa gcgttatcta agcagcgtcg
     1621 tatagttgct agggagttaa gagaccacat tgtatcggaa attcaaaact tacaaatgaa
     1681 agatgcaaat cttgagattt catttaaaaa attagaagaa ccgaatattg atggaatcga
     1741 atttgtagaa tttttaatca gtcctaataa aggggaacca ttaaaaagct taaataaaat
     1801 tgcgtcaggt ggagaacttt ctagaattat gttagcgtta aaaagtattt ttgttaaatc
     1861 gagaggtcaa actgcaattt tattcgatga ggttgactca ggtgtatctg gacaagccgc
     1921 acaaaaaatg gcagagaaaa tgcgagatat agcagaatat atacaagtta tttgtatatc
     1981 tcacttgcca caagttgcat cgatgagcga ccatcattta ttaatttcga aatcatcaaa
     2041 agatgatcgt acaacaacac aagttcaaga gttaattggc gatgataaag tagatgaaat
     2101 tgcacgaatg atttcaggtg caagtgttac tgatttaact cgtgaaaatg caagagaaat
     2161 gatacaacac aatcaaagac atagataatt tccaaagtgt gttgaatact catttatgga
     2221 tttatttatg tgcattcaaa ctaaatagac aatcatgtaa aataggtaat taatggacat
     2281 gaaaactatg tattaataaa ttttcaatgg atatatatca ttagttagga gtaagaacat
     2341 gtcagagaaa caatatgatt tagtcgttct cggtggaggt actgcaggat atgttgctgc
     2401 gatacgtgcg tctcaattag gtaaaaaagt ggcaatagta gaacgacaac tattaggagg
     2461 tacttgtctc cataaaggtt gtattcctac taaatcgctg cttaaatctg ctgaagtatt
     2521 tcaaacagta aaacaggcag caatgtttgg cgttgatgtc aaagatgcta atgttaattt
     2581 tgaaaatatg ttggcacgaa aagaagacat tattaatcaa atgtatcaag gtgtaaagca
     2641 tttaatgcaa cacaatcaca ttgacattta taatggcacg ggacgtattc taggtacatc
     2701 tatattttca cctcaaagtg gcacaatatc tgtcgaatat gaagatggtg aatcagactt
     2761 attacccaac caatttgtat taattgcaac tggttcttcg cctgcagagt tgccattctt
     2821 atcatttgat catgataaaa ttttatctag cgatgatata ttatcattaa agacgttacc
     2881 atcaagtgtt ggtattattg gtggtggtgt tatcggaatg gaatttgcat cattgatgat
     2941 agatttaggt gttgacgtaa cagttataga agctggtgaa agaattttac caactgaaag
     3001 taaacaagct tcacaactat taaaaaaatc attgtcagca cgtggtgtta aattctatga
     3061 gggaataaaa ctttctgaaa atgatatcaa tgttaacgaa gatggtgtta catttgaaat
     3121 ttcctcagac ataattaaag tagataaagt gttgctatcg attggtagaa aacctaacac
     3181 atcagatatt ggtttaaaca acactaaaat aaaactttca acatcaggac atattttaac
     3241 gaacgaattt caacaaactg aagataaaca tatttatgca gctggtgatt gtattggaaa
     3301 attacaattg gcacatgttg gatcaaaaga aggtgttgtg gctgtcgatc atatgtttga
     3361 agggaaccca atcccagtaa actataacat gatgcctaag tgtatttatt cacaacctga
     3421 aattgcttct atcggtttaa acattgagca agcaaaggca gagggaatga aagttaaaag
     3481 ttttaaagta ccatttaaag caattggtaa agcagtgatt gatagccatg acgcaaacga
     3541 agggtatagc gaaatggtga ttgatcaatc aactgaagaa attgtgggta ttaatatgat
     3601 tggtccacat gtaacagaat tgattaatga ggcatcactg ttacagttca tgaatggctc
     3661 ggcattagaa ttaggactaa caacacacgc acatccttcc atctctgaag tgttgatgga
     3721 attaggattg aaagcagaaa gtagagctat tcacgtataa ataggaggat ttaacatgat
     3781 tgattataaa tcattaggcc ttagcgaaga agacctaaaa gtaatatata aatggatgga
     3841 tttaggtaga aaaatagatg aaagactgtg gttattgaac cgtgcaggta aaattccatt
     3901 tgttgtaagt ggtcaaggac aagaagcaac tcagattggg atggcatacg cattagaaga
     3961 aggtgatatt actgcgcctt attatagaga tctagctttc gttacttata tgggtatatc
     4021 agcgtatgat acgtttttat cagcgtttgg aaagaaagac gatgttaact ctggtggaaa
     4081 acaaatgcca tcacacttta gtagtagagc taagaatatt ttgtcacaaa gttcaccggt
     4141 agcaactcaa ataccccatg ctgttggtgc tgctttagct ctaaaaatgg atggtaagaa
     4201 gaaaattgca acagcaactg ttggtgaagg tagttcaaac cagggtgact tccatgaagg
     4261 tttaaacttc gcgggtgtac acaaacttcc ttttgtatgt gtcattataa ataataaata
     4321 tgcaatttca gtacctgatt cattacaata cgcagctgaa aaattatcgg atagagcact
     4381 tggttacggt ttacatggtg aacaagtaga tggtaatgat ccattagcaa tgtataaagc
     4441 gatgaaagaa gcaagagatc gagctatttc tggtcaaggt tcaactttga ttgaagcagt
     4501 aacaagtcgt atgacagctc attcatcaga tgatgacgac caatatcgta cgaaagaaga
     4561 gcgtgaagcg cttaaaaaag cagactgcaa tgaaaagttc aaaaaagagt tgctttcagc
     4621 tggcattatc gatgatgctt ggttggcaga aatagaagcg gagcataaag acatcatcaa
     4681 taaagcgaca aaggctgctg aagacgcgcc atatccaagt gtcgaagaag cctatgcatt
     4741 tgtgtacgaa gaagggagtc ttaactaatg gctaaattat cttatttaga ggcgattcgc
     4801 caggcacaag acttagcttt acaacaaaat aaagatgtat ttattcttgg tgaagatgtt
     4861 ggtaaaaaag gtggcgtatt tggtactact caaggactgc aacaacaata tggtgaagat
     4921 agagttatcg atacgccatt agctgagtca aatattgttg gaactgccat tggtgcagct
     4981 atggtaggta aaagaccaat tgcagaaatt caatttgcag attttatttt acctgcaaca
     5041 aatcaaatta ttagtgaagc tgcgaaaatg cgttatcgtt caaataatga ttggcaatgc
     5101 ccattaacga ttcgtgcacc atttggtggc ggtgttcacg gaggactata tcattcacaa
     5161 agtattgaaa gtatatttgc ttcatcacca ggtttaacaa ttgttatacc ttcaacaccg
     5221 tatgatgcta aaggtctatt attatcttca attgaatcaa atgatcctgt tttatacttt
     5281 gaacataaaa aggcttatag attcttaaag gaagaagtgc cagaagaata ttacactgta
     5341 cctttaggaa aagcagatgt taaacgtgaa ggtgaagatt taacagtatt ttgttatgga
     5401 ttaatggtca attattgttt acaagccgct gatattttag cagcagacgg tatcaatgtt
     5461 gaagttgtag atttacgaac tgtttatcca ttagataaag aaacaattat tgatcgtgct
     5521 aaacatacag gtaaagtttt acttgttact gaagataatt tagaaggtag cattatgtca
     5581 gaagtgtcag cgattattgc agagcattgc ttgttcgatt tagatgcacc aatcatgcgt
     5641 ttagctgctc cagatgtacc atctatgcca ttttcacctg tattagaaaa tgaaattatg
     5701 atgaatccag aaaaaatctt aaataaaatg cgtgaattag cagaattcta gggagggaaa
     5761 gtcatggaaa taacaatgcc taagttaggt gagagtgttc atgaaggtac cattgaacaa
     5821 tggttagttt ctgttggtga tcatattgat gaatatgaac cattatgtga agttattaca
     5881 gataaagtga cggctgaagt cccttccacg atatcaggaa caattacaga aattttagtt
     5941 gaagcggggc agacggtagc tattgataca attatctgta aaattgaaac tgctgatgaa
     6001 aagacaaatg aaacaactga agagatacaa gcaaaagtgg atgagcatac tcagaaatct
     6061 actaaaaaag ctagttcaac agtggaacag acatctactt ctaaacaaaa tcaaccacga
     6121 aataatggtc gcttttcacc tgttgtattt aaactcgctt cagagcatga cattgattta
     6181 tcacaagttg taggtagtgg atttgaaggt cgtgtaacta agaaggattt aatgtcagtt
     6241 attgaaaatg gtggtaccac agctcaatct gacaaacaag ttcaaacaca atcaacttca
     6301 gtagatagat caagtaacca atcatctgaa gacgatagtg aaaatagcac aataccagta
     6361 aatggtgtgc gtaaagcaat tgcacaaaac atggttaata gtgtaacgga gattccacat
     6421 gcatggatga tgattgaggt tgatgctaca aaccttgtga atacgagaaa ccattataaa
     6481 aacaacttta aaaataaaga aggatataat ttaacgttct ttgctttctt tgtaaaagct
     6541 gtagcagatg ctttaaaagc atatccatta ttaaatagta gctggcaagg aaatgaaatt
     6601 gtcttacata aagacattaa tatttcaatt gctgttgctg atgaaaataa attatacgta
     6661 cctgtgatta agcatgcaga cgaaaagtca atcaaaggta tagctagaga aattaatact
     6721 ttagcaacaa aagcgcgtaa taagcaattg acaactgaag atatgcaagg tggaacattt
     6781 acagttaata atactggtac atttggttca gtatcatcaa tgggtattat aaatcaccct
     6841 caagcagcga ttttacaagt agaatcaatc gttaaaaagc cagtagtaat taatgatatg
     6901 attgcaattc gaagtatggt taatttatgt atttcaattg atcatcgtat tttagatggt
     6961 ttacaaacag gtaaatttat gaatcatatt aaacagcgta tcgaacagta tactttagaa
     7021 aatacaaata tatattagtg ataacataga tgcatctatc gacaacttgt tttatcttgt
     7081 tcttgtcgat ggatgtattt ttttggcaat aaaatatttg caatatattc aaaaaaagat
     7141 aaagaacaat aatcaacatg gttgaatgca tttttgcagt aaagtcaaaa taagacatcg
     7201 tacttgaaac atattaaaga aaacaggtga acaaattagt ttccatgatt ttaagcacaa
     7261 taatgtttgg tatattgtta aaattgtgtc taaatatagg tgtgattcag attagtttat
     7321 tgaacaatat gttattaatt agtagaatga ggatagttta aatataaagg gataggtgat
     7381 tgaacttatg gacatgaatt tcgatttata catgaacggt gttgtagaac aagcaaggaa
     7441 tgaaattgaa tctgcgggat atgagcaatt aactactgca gaagatgttg acaaagttct
     7501 taaacaagat ggtacaacac tagttatgat caattctgta tgtggttgtg caggtggtat
     7561 cgcaagacca gcagcatcac atgctttaca ttatgacgta ttacctgatc gtctagtgac
     7621 agtatttgct ggacaagata aagaagcgac acaaagagcg cgtgaatact tcgaaggtta
     7681 tgcgccttca agtccgtcat ttgcattagt aaaagatgga aatattacag aaatgattga
     7741 aagacatcaa atcgaaggtc atgatgtgat gaacgtaatt aatcaattac aaacattatt
     7801 caataaatat tgtgaagaaa gataagaggc gctaacccat gttaaagtta aatccttaca
     7861 agattggatt tagaacaata aaaacagcag tgggtatgac tttaggtgta attattagta
     7921 agctgttagg tttagataat tatgcttcaa gtgccatatt agtcgtatta tgtattaaac
     7981 atacaaaagt acattcgcta caagcgatta tttcaagatt agtatcatgt tttttagtat
     8041 tgtttttagg ttcagcaata tttagtttat taggccagag tccaattgta ctcggtatta
     8101 tcatattgtt atttatacca ttaactgtcg tattaaaagt acaagaaggt gtcattacga
     8161 gttgcgttat attacttcat gtttttaatg caaaatcaat tgatgcacat ttaattgtta
     8221 atgaaacatt attactgtta attggactaa gcattgcatt tacaatgaat ttaatgatgc
     8281 caagtttaga caaacaacta gacgaataca aatgtaaaat tgagcaacaa attgctgata
     8341 tttttagtaa atatagttat atttgtgaaa aatatgaaga taccattgcg attgaatttg
     8401 aagcgttact tttaaatatt aaaaaggcga agtctatcgc gttccgagat gttaaaaatc
     8461 attttgttag aaacgaaaat tcatactatc attattttga tatgcgagaa gagcaagtgg
     8521 aattgttaat gagaatgaaa ccgctcatcg aaagtatctg tcataaagat ccacttttaa
     8581 atgatgttgc gtcactgctg aaagaaattg gtctaaatgt gaatagtaat gactatacag
     8641 caatgcgttt acacaatttg tatgagacgc gtttgaaatt ggatgaactg ccattgccaa
     8701 caacacacaa agcgcttaac tcgagagcaa gtattattca aatattaaat gaattagaag
     8761 aatatttaca aatcaaatcg caatttggtt cattgaaaat gcatagtgaa gtagcttaaa
     8821 aaataatcca tccgtatgac ccatgtttga aagtaatcgt tgcaatacga taacttatta
     8881 atacatgcgt ttataacgga tggattttta attgtatgat tttgtatagg tagttgagat
     8941 atggtttata aaattggaat gatactcgtt aataacaatg ctaatacaac tgatacaagc
     9001 ataacgataa tgataattct taaaacttta ttttgcattt tgtgtgcctc caaatgaata
     9061 acctttgtat taataattac gacataagat ataaaatttt taacttgaga cgatataact
     9121 agcaatcatc ataaaggaat attataaaaa acatgagtaa gtttgcttat gtcattttcc
     9181 tcattatacg atatctttga aaaatcaatc aatatatttt tgacaaagct tagtttgagt
     9241 tacaataaat atatatatta aattgaaaat aatatgacac aatttacaag cctgataaat
     9301 ctgtgtaacg tcaattactg taacatttta attcatcaat ttttaccgtt tggcgtactt
     9361 gaaaattttg ataaaagggg ttccattatg attaatgagc aaagattact taatacgttt
     9421 ttagaacttg tacaaatcga ttctgagaca gggaatgaat caacaatcca acctatttta
     9481 aaagaaaaat tcatatcatt agggttagat gttaaagaag atgaagcggc taaacatcct
     9541 aaattaggtg ccaataatct agtgtgtaca atgaaaagta ctatcgaaga aggcgaagta
     9601 cctaaattat atctaacgag ccatatggat actgttgtac ccgcaattaa tgtaaagcca
     9661 attgtaaaag atgacggcta catatactct gacggtacga ctattctagg tgcagatgat
     9721 aaagcaggat tagcagctat gcttgaagtt ttgcaagtga taaaggaaca acaaatccca
     9781 catggacaaa ttcaatttgt gattactgtt ggggaagaat caggtttaat tggtgctaaa
     9841 gaattaaatt cagagttgtt ggacgcggac tttggttatg ctattgatgc aagtgctgat
     9901 gtcggtacta cggttgtagg tgcaccgacg caaatgttaa tttcagctaa aatttttggc
     9961 aaaacggctc atgcaagtac gccaaaagag ggtgttagtg ctattaatat tgcggcgaaa
    10021 gcaattagtc gaatgaaatt aggacaagtt gatgaaatca ctactgccaa tattggtaag
    10081 tttcatggcg gttcagctac gaatattgtt gcagatgaag tcattttaga agcagaggca
    10141 cgttctcacg atccagaaag aataaaaaca caagtcaaac atatgactga tgtgtttgaa
    10201 actactgcat cagaattagg tgggaaagca gtagttacag tggagcaaag ttatccagga
    10261 tttaaaatta atgataatga aacggttgtt aaaattgcac aagaaagtgc tcgtaattta
    10321 ggattatctg caaatacaat tatttctggt ggtggttcag acggcagtat tattaataca
    10381 tttggtattc cttcagtcat tttaggagtg ggttatgaaa aaatccatac aacaaatgag
    10441 agaatgccga ttaaatcatt gaacttgctt gcaagtcaag tgttagaaat aatcaaaata
    10501 gttgcgcgac aatcgaaatg atttagtgta gtgtatgtct attttgtgat acaatactaa
    10561 tgaaaaatca acaagtgagg taagtaatat gacacaacaa attggagtta ttggtttagc
    10621 cgttatgggt aaaaacctag cttggaatat tgaatcacgc ggatatagtg tatctgtatt
    10681 taaccgctca agtgaaaaaa ctgatttaat ggttgaagaa tcaaaaggga aaaatattca
    10741 tccaacgtat tcattggaag aatttgttaa ttctttagaa aaaccacgta aaattttatt
    10801 aatggttcaa gcaggtaaag ctacggatgc aacgattgat agtttgttac ctttattaga
    10861 tgatggagat attttaattg atggtggtaa tacaaactat caagatacta tcagacgtaa
    10921 taaagcatta gcacaaagtg caatcaactt tattggtatg ggcgtttctg gtggtgaaat
    10981 tggagcgtta actggtccgt cattaatgcc gggtggacaa gaagaagctt ataacaaagt
    11041 tgcagatatt cttgatgcaa ttgctgcaaa ggcaaaagac ggtgcttcat gtgttactta
    11101 tattggacca aatggtgctg gacactatgt gaaaatggta cacaatggta tcgaatatgc
    11161 tgacatgcaa ttaattgcag aaagttatgc tatgatgaaa gaattattag gcatgtctca
    11221 tgaagacatt gctcaaacgt tcaaagactg gaatgctggt gaattagaaa gttatttaat
    11281 tgaaatcact ggtgatattt tcatgaaatt agatgaaaat aaagaagcat tagttgaaaa
    11341 aattcttgat actgcaggtc aaaaaggtac aggaaaatgg acttcaatta acgcattaga
    11401 attaggtatt ccattaacaa tcataactga atctgtattt gcacgtttta tctcttcaat
    11461 caaagaagag cgcgtaaatg cttctaaaga attaaatggt ccaaaagcat catttgatgg
    11521 tgacaagaaa gactttttag aaaaaattcg taaagcatta tatatgagta aaatttgttc
    11581 ctatgcacaa ggtttcgcgc aaatgcgtaa agcaagtgaa gataatgaat ggaatttaaa
    11641 acttggtgat ttagctatga tttggagaga aggttgcatt atccgtgcac aattcttaca
    11701 aaagatcaaa gatgcttacg ataataatcc tggattacaa aacttattgc tagatcctta
    11761 ctttaaaaac atcgttactg aatatcaaga tgcgttacgt gatgttgtcg caactggtgt
    11821 tcaaaatggt gtaccaacac ctggtttctc atcaagtatt aattactatg atagttaccg
    11881 tgcagctgat ttacctgcaa acttaattca agcacaacgt gactacttcg gtgctcacac
    11941 gtacgaaaga aaagataaag aaggcgtatt ccatacacaa tggattgaag aataatattg
    12001 actattgagc ctgagacctt atgaatttag gtaatgttaa agagacaatt tctattattt
    12061 atttgataga aattgtctct ttccttatgt atttatcgaa atttactacg catgatatat
    12121 tcaatgattt tctttctctt gtcaaattaa aatttttaac ttttataaat taacgatatc
    12181 tatcctcata gaatttgtcc gtttaagtgt agacgtttta cttacatact attaagatag
    12241 cattagttaa taattttaat atttagataa atagattacg ttttattatt tgatgaagaa
    12301 gagggataga atgtcatatg cttctgtatt gtttaataag aactttactt tttatttaat
    12361 agggatagct ttttctaaca taggcagtgc ttttactacg tttgttttcc gttaatgatt
    12421 ttaaagctta ctggctctgc atttcaagtt ggcatagttt ctgcactaag ttttatacct
    12481 tatgcaattt taggactgcc agcaggtgct ttaattgaca gattgaataa gaagaccata
    12541 atgaaatgtg ctgatataat taggctcatt tcatatttaa gtataccagt attaagtttt
    12601 tataatatgt taagcatctt tcaaatttat gtagttgcaa tcatttcagg tattggatta
    12661 gtatttcaca gtatttctga ggtttcgata atcccaagta ttgtaaaaga agaagatttg
    12721 gcgagcgcaa acagttatat atacgctact caaaatgttt cggaattttt gggccctata
    12781 attggaggtt tactttatac atatatggga tattcaatat tgatttttat agattctatg
    12841 acttttttac tctcattttt cagtttgata ttaattaaaa ttgaaacaaa gagtatattc
    12901 aatcaagaaa agttgagtgg aaaaaatttt ttgagcgatg ttaaagttgg atttgattat
    12961 ctattatcta attcaactat tagagtaatg gctgttgttg taagtttatc caatctaatt
    13021 atctcacctt attatattta tatagtaatg tttgtaaagg aagacatgaa tcaatcatct
    13081 caagcattgg ggttagtttt aggtatctca tcattaggtg cgctaatcgg ttcattatct
    13141 gcaagttttc tcttgaaatt atttaatttc ggaaaactaa ttgtaattat attattttta
    13201 gatacaattt ttagattaat gttaccattt agtacttata tttttatact catcccactt
    13261 ttagggttga catatatgac acaatccatt ttaaatatag cgataatcac actgaggcaa
    13321 aagaaatgtt cagaacatat gttaggacgt gttaatagtg tttttaaaac aatggttttt
    13381 gcattcagag ctataggcct ttttttaggc ggcatattac ttgaaaataa aggtggattt
    13441 tatgcactta cgatcagcgc tgtgttattt ataccattag tgttatatat actgaaaaat
    13501 agattttata aagtagaaga atagtaagga gaaaattaat gaaaaacagc caaaatattg
    13561 tttgtattgg tgcagcaaat atagataaac aattatattt aaaatctgaa ttggttaata
    13621 aaagttcaaa ccctgtatct tcgataagcg tatctggagg tgtagctagg aatttagcgt
    13681 ataatattgg caaattaagt aataaaaaaa ctattctatt aactttatta ggtaatgata
    13741 aagaagctga ttatatttta aatgaagcta ataagtttat cgatttaaaa catataaaaa
    13801 ttatagaaaa tatgaacaca ggaatgttca cttctgtaat agattataat ggggaaatga
    13861 tatgtggttt tgcagatatg gatatatata acttaataaa taacaaatat ttaaatgaaa
    13921 aaatagaagt aataaaaaat gcagattgtg tggttgtaga ctctaatatt cccaaggaaa
    13981 gtatacaata tttaattcag ttgtgtggaa agagtaatat tccaataggt ataatcccag
    14041 catctataaa gaaggtgaga aacatactaa aaaacttagc aaagatagac tatttaatat
    14101 ttaataaaaa agaagctgaa gctttttgga acgtttcttt aaatactaaa agtgattata
    14161 aaaaaataaa aaaaattagt aaaacttcag aaataaaaaa tatagttatc actaatggaa
    14221 atgatgatat cttttataca aatggagaat taagcatatt taaaaatgtt caacagagtt
    14281 caaatataat taatgacaca ggtgcaggag atgttttttc agcatcagta tttcattatt
    14341 ggcttgaaaa ctatgaagtt gataattact tagaaatggc attgtttaat gcctgttcta
    14401 cagtgagcac tatatataat acttccatga ttaattagca acacatgtaa atggcaacta
    14461 taaaaatggc tgctatgtca aattggactg atgaattcaa tataggaagt taggcaacta
    14521 aacattgtat aactttcttt ttagttattg gagcgtaaag ttttgaacat aataatattc
    14581 gattgcgcaa atgattgtaa cttccataat caaatcatgt acgtttaatt aattttatta
    14641 tgttagttat acattctaat ggacaatgtg agctacaccc agttaagttg acatagaaaa
    14701 tacgaaaact ttgaacgaag tatttcattg atatatgtcc cagatgacat gtttgattaa
    14761 tgaagttcta tgtggctaca acatataaaa aattacacaa gtattatctt tgaccttaaa
    14821 agcatcccgc catattttta agcattttgt gaagtaataa aatatttact gtgttatgat
    14881 agcatgtact atagagaaat agaggtacta ttttatggaa aagttaaatc aagttatgtt
    14941 atatgttgat gatgttgaaa aagcgaaagc attttggaca gaaattttag aatttgttgt
    15001 tgtaagtgaa acaccgttag ctgaggacta tgttgcagtt gaagtatcac ctacgaaaga
    15061 tgctgaaacg tcattgacga ttatggcaaa agaatttatt gaaaagtata gcccggaggt
    15121 gaacctagga acaccatctt taatgtttaa agagaaaaac tttgatgcat tatactcaaa
    15181 attaaatgat ttaggtttaa ctgggcatga tatagtagaa atgaatggtc aacgtgtatt
    15241 taatttccaa gatggccaag gtaattattt tgcagttagt gactaaacaa tcgattttaa
    15301 tatttataca aacctttata ttgattttcg gattgaattt cagtatgaag gttttatttt
    15361 tatcgtttta tgcaatcgtt tgcacaaatc ttttgacttt tttagaaagt gtgactaaac
    15421 tggtattata caaaggttgg aagcgttttc cagtacagag attaacagga ggtgattaaa
    15481 tggttacgat taaagatgtt gcactaaaag ccggtgtttc tccttcgaca gtttcaagag
    15541 ttataaaagg aaatcaacgt ataagcgaag cgacaatttc aaaagtgaag aaagttatgg
    15601 aagaattgaa ttattttcct aataccgctg ctagaacttt aattactaac caaacatata
    15661 aaattggttt agtgttaaaa gggtctgagg agcctattcg actgaatcca ttctacatca
    15721 atgtattgct aggaatttct gaaacgtgta accagcatgg ctatggtaca caaacgacag
    15781 tctcaaataa tatgaatgat ttaatggatg aagtttataa aatgattaaa caacgaatgg
    15841 ttgatgcgtt tatactgctc tattcaaaag aaaatgatcc gattaaacaa atgttaattg
    15901 atgaaagtat gccatttatt gtgattggta agcctacatc ggatatagat catcaattta
    15961 cacatataga taatgataat atattagctt ctgaaaattt gacacgacat gttattgaac
    16021 aaggtgtaga tgaattaata tttattacag aaaaaggcga ttttgaagtt tcgaaagaca
    16081 ggattcaagg atttgaaaca gttgcatcac aatttaatct ggattatcaa attattgaga
    16141 ctagtaatga aagagaagtt atttttaatt acatgcaaaa tctgcatacg cgtttgaagg
    16201 acccaaatat taaacaggca atcatttcgt tagatgctat gttacattta gcgattttaa
    16261 gtgtcctata tgaacttaat attgaaattc cgaaagatgt aatgacagca acgttcaatg
    16321 attcttattt aaccgaaatt gcgtcaccac ctcaaaccag tattgacatc aagcctcgaa
    16381 tgttaggtca acaggctggt gcagcaattt taaatatatt aaaaaataaa gcacaggatg
    16441 ttattgaact cgtcattata gatacagaat taaaaataag aaaatcaaca cagcgatagc
    16501 aaaggagttt taaaatgaat aagcaatggt ggaaagaagc agtagcatat caagtatatc
    16561 caagaagttt taatgatagt aatcacgatg gtattgggga tttacctgga atgattgata
    16621 aattggacta cttaaaagat ttaggtatcg atgtcatttg gctcagtcca atgtttaaat
    16681 cacctaatga tgataatggt tatgatatta gtgactacca agagattatg gatgaatttg
    16741 gaacgatgga agactttgat cgtttattaa aaggtgttca tgatagaggt atgaagctta
    16801 ttttagattt agttgtgaat catacatctg atgaacatcc ttggtttata gaatccaaat
    16861 ctagtaaaga caatcccaaa cgtgattggt acatttggca agatccaaag ccagatggct
    16921 ctgaacctaa caactgggaa agtatattta atggatctac atgggaatat gatgctaata
    16981 ctgagcaata ttatttccat ctattcagta aaaaacaacc tgatttgaat tggggtaatc
    17041 cggaagttag agatgctgta tttgaaatga tgaactggtg gtttgataaa ggcattgatg
    17101 gatttagagt agatgcaatt acgcatatta agaagacgtt tgaagcgggt gacttacctg
    17161 tacctgaggg taaaacatat gccccagcat ttgatgtaga tatgaatcag ccaggtatac
    17221 aaacttggtt acaagagatg aaagatcgct cattaagtaa gtatgacatt atgactgttg
    17281 gtgaagcgaa tggtgtaagc cctgatgatg ctgatgactg ggtcggggaa gaaaatggta
    17341 aatttaatat gatattccaa tttgaacatt tgggactgtg gaatagtggt gattctcact
    17401 ttgatgtaaa ttcgtataag tctgtattaa atagatggca aaaacaactt gaaaataaag
    17461 gttggaatgc gttgtttatt gaaaatcatg accaaccacg acgtgtatcg acgtggggtg
    17521 acgatgacaa gtattggtat gaatcagcaa caagtcatgc agcagtttat ttcttgcaac
    17581 aaggtacgcc attcatttat caaggtcaag aaattggtat gacgaattat ccatttgaaa
    17641 gtattgaaac atttaacgat gttgctgtta aaaatgacta tcaaattgtg aaagctcaag
    17701 gtggagatgt agacgcttta cttgcgaaat ataaagatga gaaccgagat aattctcgca
    17761 caccaatgca atgggatgat acgttaaatg gaggatttac aaatggtgaa ccgtggttcc
    17821 cagtgaatcc gaattataaa actatcaatg ttgcacaaca attagaagat gaggattcag
    17881 tattacaatt ttataaagat ttaattcaat taagaaagtc taatgatgta tacgtatatg
    17941 gtcaatttga tttagtagat gctgaaaatt cacaagtttt tgcgtacacg agaacattaa
    18001 atgaaaagca agttcttatt gtaggtaatc ttactaacca cgaagcagaa ttaactgtac
    18061 catttgatat aagtcatgga gaagtgaagc tatttaatta tgatgccaaa gttaatttaa
    18121 aacagttact tccatatgaa gcatgtgtta tcgaactaaa ttaacatatt aatatgcccc
    18181 ctaatagaat ctaaggccat ttgtagacct atagatttat tagggggctt ctttgttttt
    18241 aattatttaa tcttcgtcat aaggactctg tttaacagga atccaaagct gaattttagt
    18301 gaatgggtca ttaaatgaaa tatcgagagg gtacacttca acatataaat catttcgttc
    18361 atatggcaat gttaactgca aacttgattc aatatagtac caagcttcat tagttgcata
    18421 atcaatttca ccttgtaaat tgaatttcgc acaatgtttg ccaggtaaaa atcgactttc
    18481 taagtgtgca ggataacgtt cacttggtac acctacaaat atttctaaac cattttcaag
    18541 aggacaacta ataacaaata gttcaaatgg actaacgtca ttatatcgtc gtaactcttt
    18601 aattttacca tcaatgagca agtcttctaa aaaatccgga acattaaaag gatgtgacaa
    18661 atacttagtg tctataaatc gtgcatatcc aaccaatgaa atatcatctg tctcttctaa
    18721 tctgtatgga taaggtgctc tctcagtagt tgataatttg atatataatc gctcttgaat
    18781 ttgtaattca tcttttttag tagaagcttg aataggtgat acgccgtgaa aatcactaaa
    18841 atcatttgca aaatcatttg aatttgcata gtgatatttt ttagcgatat ctacaagtcg
    18901 tgtagcacca ttaatcacat catttgcagc gagtgtcatt ttacgcgcgc gtgcataagc
    18961 ttctggagat aagccgacaa tcattttaaa tgattgatca agatggtatg gcgaaagacc
    19021 aacgtaatca cttaattctt gcaaattgaa aggctctaat aaacgatctt caatataaac
    19081 aattgcctgt tgtatttgct tgataacgtc caaaactttc actccaatta attcaatcat
    19141 ttatttttat tctacattat ttctataaat tatacaccca tttgttcaat gattattaaa
    19201 atagttttgg gcattgtaaa atataatttc ataatatagt ctagaaaaaa agcgaatgat
    19261 agaacaattg atatacttga ttcgtaatca atccttgtca ttcgctcatt tatttttgtt
    19321 taacatgtgc gctttaattc aattattgaa tatcgtccca ccaatggtta ccatcacgag
    19381 caagtaataa atcactttct aatggaccat tagtacctga ttcatagtta gggaattctg
    19441 gatcaaccat attccattca tcttgaattg catcaacaaa tttccatgtt gattttaatt
    19501 cttcccagtg cgtaaagtta gtggcatcac ctttaagaca atcgaataat agattttcat
    19561 atgcatctac agtattcatt ttatcttgag cgctcattga ataagacaat tgaacaggtt
    19621 cagtttcgat accttgtgta tttttcttag catttagatg taaagataca ccttcatttg
    19681 gttgaatatt gattactaat aggtttgaat ctaacagttt atcagtttca tagtataagt
    19741 tcattggtac ttctttaaat tcaacgacaa cttgaattgt tttagatttc atacgtttac
    19801 cagtacggat atagaatggt acgccagccc atctaaagtt atcaattgtt aatttacctg
    19861 aaacaaatgt aggtgtgtta gagtcatctg caacgcgatc ttcatcacgg tatgctttaa
    19921 cttgtttacc atcgatatag ccttcgccat attgaccacg aacaaagttc tttttaacat
    19981 cttcagattg gaaatgacgc agtgatttaa gtacttttac tttctcagca cggatatctt
    20041 cactatttaa actaataggt gcttccatag ctaataatgc aaccatttgt aacatgtggt
    20101 tttgcaccat atcttttagc gcaccacttg attcataata accaccacga tcttcaacac
    20161 ctagtatttc agaagatgta acttggatgt ttgaaatata tttgttattc cataatggtt
    20221 caaacatcgc attcgcaaaa cgtaatacct cgatattttg aaccatgtct tttcctaaat
    20281 agtggtcaat acgataaatt tcttcttctt taaatgattt acgaatttga ttgtttaatg
    20341 cttcggctga ttttaaatca ctaccgaatg gtttttcgat aacaaggcgt ttaaatcctt
    20401 ttgtatcagt aagaccagaa gattttagat aatcagaaat aacgccaaag aattgtggtg
    20461 ccattgctaa atagaatagt cgattacctt ttaattcaaa ttggctgtct aattcattac
    20521 taaaatctaa taattcttga tagctttctt cattactaac atcatgtcta tgatagaaga
    20581 catgttccat aaacgcgtca attttgtttg tatcttttac gtgcttttga attgatgatt
    20641 ttacttgatt acggaaatca tcattagtaa tgtcacgacg tccaataccg atgatggcaa
    20701 tatgttcatc taaattgtct tgttggtaga gatggaatat tgatggaaac aacttacgat
    20761 ggcttaagtc accagttgca ccaaagattg tgattaaaca agggatgtgt ttgtttttag
    20821 tactcaagat taaaacctca attcttaatt agatatatga tttattatta taagataatc
    20881 cattcagcag gtcattcaat atgctcgaca gtatgcagtg tcttaaatga aatatcgatt
    20941 catgtatcat gtttaatgtg atacttatta atgataagta taacgtaatt atcaaaattt
    21001 atatagttat gtctacgtta aagttagaaa aattaactag caaagacgaa tttttaacag
    21061 attttgattc aagtataaat taaaactaaa ttgatacaaa ttttatgata aaatgaattg
    21121 aagaaaagga ggggcatata tggaagttac attttttgga acgagtgcag gtttgcctac
    21181 aaaagagaga aatacacaag caatcgcctt aaatttagaa ccatattcca attccatatg
    21241 gcttttcgac gttggtgaag gtacacagca ccaaatttta catcatgcaa ttaaattagg
    21301 aaaagtgaca catatattta ttactcatat gcatggcgat catatttttg gtttgccagg
    21361 attactttct agtcgttctt ttcagggcgg tgaacaaaag ccgcttacat tagttggacc
    21421 aaaaggaatt aaagcatatg tggaaatgtc tatgaattta tcagagtcac atttaaacta
    21481 tccaattaca tatattgaaa tcgatgatca tctaacatat catcatgatg gttttactgt
    21541 agaggcgcat ttacttaatc atggtattcc atcatatgga tatagagtaa tggcacctga
    21601 aactacaggt acaatcaatg ttgaagcgtt aaaaaacatt ggtcttgaac caggtcctaa
    21661 atatcaagaa gtaaaatctc atgatacatt tgaacataat ggtcaagttt atcaatcaaa
    21721 agattttaga ggtgagtcca aacagggtcc aattgtagca atttttggtg atacaaaacc
    21781 atgttctaat gaacgtgtga tttctagaga tgctgatgtg atggtacatg aagcgaccta
    21841 tatagatggt gaaaagcatt tagccaacaa ttatcatcat agccatattg aagacgtatt
    21901 tgcattgata aaagaagcca atgttaaacg tacgttgatt acacatttga gcaatcgcta
    21961 taatacagaa gatataaatg aaatttatca aacattgata caaaatgaag atacgccgaa
    22021 ttttaatttt gtgaaagatt ttgatagttt taaaatctaa tgacaaagta ataaacaatg
    22081 cttgccaaat agtaaaaatt tataaaaata acccgtcaaa cacagtgctt gcgatgatgc
    22141 atacatgtgt tggcgggttt gtttttattg gtcttctata ttagaaagtt caatactacg
    22201 gtcgacggca gcatttagac aatcttcgaa aatagatact aaatcatatt gtgacaatgt
    22261 atcaaggcca gcttgtgtcg taccaccttt agaggtaata ttttttctta attgagccat
    22321 gctcaaatct gaacgttcaa tcatcttact tgtacctata ataaggttgc gtatagattc
    22381 ttcaacttgt tctttttcta gtccaagttt cgtaccagct ttaacgtatt gctcgaatac
    22441 atgatataaa aatgctgggc cgcttccggt gatagctgtt acttgatgta aatgatcctc
    22501 tgatacttca attactgaac caaatgcttt aactaaatcg ttaatttcat ctttagattt
    22561 agggtcaaag ttgtttgaaa aactaatgcc agtaactgag tgtccaactt gcgcatttgt
    22621 gtttggcata attctagcaa ctggattttg gcattctaat tgctgtttaa tataatcaat
    22681 cggaatacct gccataattg aaatgaagca attgtctttt gtaatatgtg gtttgatgcg
    22741 tgttgctaga gcatcaaagt catgtggttt ggtacctaaa aatacataat ctgcatcttt
    22801 taataatgtc gcatcatcat aactatagtt aacacctagt ttttcagcga atgcttttaa
    22861 agcttgttca ttagatttat ttgttaaata tatatcattg gcatctaagt tgcttgagtt
    22921 aataattcct gtaaatatag cttgtgccat attaccagct ccataaaata cgagtttcat
    22981 taacgtcaca tccctttagt ttttgatttg tatgttatat tttcttatta ttgtatatat
    23041 attaggaaag ataatcaact tgtctcaatt atgtaaagtt gataaattgc attctatata
    23101 atggaaaagg aaggttaatt gatgaaagga caacatttta tagtgactgg tggtacgagt
    23161 ggtttaggtt tatcgattgt ccgaaagtta ctagaaaata aagtgcatgt aacgttgttg
    23221 gtgcgtgatg ttgataaagc aactagaatc tttgaccagg aattggggaa aactataaat
    23281 gttattcctt gtgacttaaa tgatttgaag tcgatacagg cactgcaatt tgaagacaat
    23341 acattattcg atggattcat ttatagtgca ggtttaggtt atttcaaatc tatttcagac
    23401 catagttttt cagaaatgat cgaaacgtat cagcttaatt taattagttt taatgtttta
    23461 tatactgtgt tgagaccata tttaacatct aacgcacata ttgtaggtat ttcaagtcaa
    23521 gctgcatttt ctacacaagc aaatgctgca cattatgggg catcgaaagc agggttttat
    23581 gcattaatga atgctttaag actagagtca ccaaacttac atatcatgac agttaatgtt
    23641 ggcccgattg atacaccttt tcatcaaaaa gccgacccat caatgaagta tgctaaaaag
    23701 atggggaaaa ttatgcttga tgctaatcag cttgcagaag atatcattta tggcattaaa
    23761 acaaaacaat tggaaattaa tcggcctaaa tggatgcatc atgcgttgaa aatgtatcaa
    23821 attgcgccta gattttttga acgatgtttc cctaaattat ttaaaaataa agcataaata
    23881 ggtgggatgg caatgaactg tcaatcggag agtgtggtaa gactttgtgt acgatatgcg
    23941 gaacaactat cagtatttga agaatttaca gtgctggata ttttaagcga tatttctgta
    24001 gaccaaattt cagacagtac attatattat acttgtgaaa aattcaaact acttgtgttg
    24061 caaggaaatg tgttgggtgt acaagtaata actaataatg atgaattaac atgcgaagtg
    24121 aaatatagaa agatgtttta ataaaattat taaaagatga agtggcagaa gtttgcttaa
    24181 ggcactgccg cagggcggaa ttactaaata ctgatgcgta aggacaactc tttaattact
    24241 attataagtg agcaatctta atcataaatt atagcgaatt ttacataaac aacagcagta
    24301 aggcattttt ataatgaaac gcctcatact gctgttttta ttactgaaaa tgacttctac
    24361 actaaatgat tggtatattc caaatccttt acacgagctc ttgctgtttt aatctgatct
    24421 aaactaactt tagtatgata gttttcaata ttttctttta attggtcgac gctacttgca
    24481 cctacaatga tggaaccaag cacgtcatgt gatgttaaat aactaaatgt caatgcagat
    24541 aaattacttt caatttcttt aatagaggct attgtttcac ccaattcatc atgagaataa
    24601 tcaaaaatac catctttaaa tttattgtcg agcacattaa cactgtttga agttaataat
    24661 cctttagaca caggtcctct tgccaatact ttaacatcat cgttgtgaat tgcatctaat
    24721 aatgattctg gacgattatc aatcaaattg aattgagaca ttatcgtttc gatttgacta
    24781 tgttttaaat aataatcaat tacatttggg cgaatagaag aaataccgta agcacgtata
    24841 attccttctt gtttcaattc atcaaatgcg cttattgttt cgtctaatgg atcatcaatg
    24901 gttccgccat gaagttgata taaatcgata tgatcaatac ctaaacgctt tagtgaacct
    24961 ttaaccgcct ctttaatata ggatttactc ggatcccatg ttgtactgcc atcttttgtt
    25021 aaacgattgc ctacttttgt accaatgaca atatcatcgc gttgttgata tttaaaaagt
    25081 gccttaccaa caactgattc atttatacct ttatcgtaca tgtctgctgt atcaaaataa
    25141 gtgataccat tttcaacagc acaatctata atttgttcgg ctttttttaa atctgtgcct
    25201 aaactcatgc aacctagacc taattcagat aatgcaatac cactttttaa tatatttttt
    25261 tgcatgtctg ttctcctttc gatacacttt agctaacaag catagtgtat caatatataa
    25321 tatgaattta aaggaacgtg attataatgg atttaaatga aaaaacaatt gatcgaacag
    25381 ttatttataa tggcaaaatt gtagatgtag aaattcatac agtgacatta ccaaacggtg
    25441 aaacgtcaac aagagaatta gtttatcata atggtgcagt tgctgtttgt gcattgacac
    25501 ctaaaaaaga agtcgtatta gtgaaacagt atcgtaaacc agtagaaaaa ccattgttag
    25561 aaattccagc tggtaaatta gaagatgatg aagatagagt cgaagcggct aagcgtgaat
    25621 tagaagaaga aacaggatat attgccaaag agttaacaca tgttgtagat atgtatggtt
    25681 caccaggttt ttgtgatgaa caattatcaa tatattttac ggataatgta gaagaaggca
    25741 cagttcactt agatgaagat gaatttgtcg aagtcattaa agttccgatt gagaatgtta
    25801 aatcgatgct aatgaataaa gaaatagaag atgcgaagac tatcattgca ttgcaacacc
    25861 tattattaaa ttataatcat tctaaataac aaatgagaaa agcttgcatt ttattgagaa
    25921 aactgttagt tttaattgtg aagtttgaaa taatttgtaa tgattttaat tattagtagg
    25981 ggagtggaca tcgttggaag aacgattaaa tcgcgttaag caacaattac aacaatcatc
    26041 atataagcta acgccacaac gcgaagctac tgttagagtt ctaattgaaa atgaaaaaga
    26101 tcatctaagt gctgaagacg tatatctgaa agtaaaagac aaagcgcctg aaattggctt
    26161 ggcgacagta tacagaacgt tagagttgtt agctgaacta aaagttgtcg acaaaattaa
    26221 ctttggtgat ggcgtcgctc gttttgattt aagaaaagaa ggcgcaaaac atttccacca
    26281 tcatttagta tgtatggaat gtggtcgtgt agatgaaatc gatgaagatt tgttaccaga
    26341 agttgaaaat cgagttgaaa atgagttcaa ttttaaaatt ttagatcatc gtttaacttt
    26401 ccatggtgtg tgtgaaacgt gccaagctaa aggtaaagga tagtaaattg cgtaggttaa
    26461 attaaccttc gcttttttta gaggtgtggt tatggaaaca attattgaag aatatttgcg
    26521 ttttatacaa attgaaaaag gactaagttc taacacaatt ggtgcttata gacgtgattt
    26581 gaaaaagtat caagattata tgactgaaca tcatatctcg catattgatt ttatagatcg
    26641 acaattaatt caagagtgtt tggggcattt aatagaccaa gggcaatctg ctaaatccat
    26701 tgcgcgattt atttcaacaa tccgtagttt tcatcaattt gctataagag aaaaatatgc
    26761 ggcgaaagat ccaacggtat tattagattc accaaaatat gacaaaaaat tgcctgacgt
    26821 tttaaatgtt gacgaagtgt tggttttatt agaaacgccg gatttaaata aaattaatgg
    26881 ttatcgtgat cgtacgatgt tagaacttct gtacgcaacg ggaatgcgtg tatctgaatt
    26941 gatacattta gagctagaga acgtgaactt aataatgggg tttgtacgcg tatttggtaa
    27001 aggcgataaa gaaagaattg tgccattagg cgacgcagtc attgagtact taactactta
    27061 tattgaaacg attagaccgc aacttttaaa aaagactgtt actgaagtct tatttttaaa
    27121 tatgcatggt aaacctttat cacgacaagc aatatggaaa atgattaaac aaaatggtgt
    27181 aaaggcaaac attaaaaaga cgctaacgcc acatacgttg cgccattctt ttgcgacaca
    27241 tttattggaa aatggcgcag atttaagagc agtgcaagag atgttaggtc actctgacat
    27301 atctactacc caactctata cacatgtttc aaaatctcaa attagaaaaa tgtataacca
    27361 atttcatcct agagcataaa gtgaaccata actcaaaagt cacaatacac atgactaaaa
    27421 atgtctgtgc tattgtggct tttgagttat ggttgattaa ttacgtctat gttttcttaa
    27481 ttgaatcgct tcttcttttg ctgcaatcac ttctgaacga tcacgccgca tgtgatggtc
    27541 tacaataaaa ggatctgttg ctgtttcgtg attataatca taatctggat agttggcctt
    27601 gatgatgcgc tcaaagtctg gagttattgg taatataaca gatgaaaaag gctttgctgc
    27661 attcaatttt gcaatctgtt tctcaattaa caactgataa tcatttaaat taaggtataa
    27721 cgcatctcta tctttagcat tttgtattat ttctttagat ttattaaaag acttataggc
    27781 gccttttaaa ttattgcggc gataatggta acaagcagtt gcaaacaaga ttaaactaac
    27841 aactgcatct tgcttactgt agttattttc agctttccat gcatcttcta aaatgtcatg
    27901 acataggaaa taatgttgct tagtatgaaa ttgataatag aaatttatca gtgcctgttg
    27961 cattttgtta tcaccccaat ttaaaagtaa gttattttca tgctataata ttttagagaa
    28021 ttatgcacat atgacgcaat acgaggtaga tattatgtat gaagttaaat tagatgcttt
    28081 caatggacca ttagatttat tactgcatct tatccaaaaa tttgaaatag atatttatga
    28141 tattcctatg caagcattaa cagagcagta tatgcagtac gttcatgcaa tgaaacagct
    28201 tgaaattaat attgcaagtg aatacctagt attagcgtca gaactcttaa tgattaaaag
    28261 taagatgcta ttaccacaat caacatcaga tatagatgtt gatgatgacc cacgggaaga
    28321 tttagttggg cgtttaatag aatatcaaaa ttataaagaa tatactgcta ttttaaatga
    28381 catgaaagaa gaaagagatt tttattttac aaaaagaccg acagagttat ctcatttgga
    28441 aacagatgaa tcttgggatc caaatcatac gattgactta actgaattaa ttgtagctta
    28501 tcaaagagtt aaaaatagag ttgagttaaa tacacctaaa tctgttgaaa tccgaaaaga
    28561 gacatttacc attcaacaag ctacagaaca agtgacatcg agattgaaag ataaagatca
    28621 ttttaacttc tttagtctgt ttacgttttc tgagccaatt gaacaagtag tcactcactt
    28681 tttagctatt ttagagatgt caaaagcagg aataattaat attgagcaac aacgtaattt
    28741 tgaagatatt aacattgtta gaggagtgaa ctaccatttt ggataatcat ggtatattag
    28801 agtcgctatt atttacagct ggcgatgaag gtttagatga aaaacagcta ttagaaattt
    28861 tggatatatc gaaagaccaa ctcgtagaat taattgaaaa ttattcatca catggattaa
    28921 taatacaacg atttggaacg acgtatgttt taacgactaa aaaagaagcg tcaatgtata
    28981 ttgaacaatt aattgaacaa aagtcacaaa tgaaattatc acaagcagca atggaagtac
    29041 tatcaattat tgcttataac caaccattat caagaagtga tattgaatta attcgtagta
    29101 tcaattcaga tggtgcggtc aagacattga ttgccaaagg actagttgag gctaaagtgg
    29161 tcaatgaaca gcgtagccaa caattaatta ctactgattt atttttaaat gtatttggta
    29221 tttccaatat agaagatttg ccgacaactg aagaagacga tgaagaaatg gatgcttttt
    29281 tcagtaatct agtcaatcaa aaaggagaaa ataatgacta aagaattaga acgattacaa
    29341 aaacgtatag ctaatagcgg ttatacttca agacgtaaag cagaaacttt aatttctgaa
    29401 ggaaaagtga aagtgaatgg tacgactgtc actgaattag gaacaaaagt gaagccatca
    29461 gatacgattg aagttgaagg tattaaaata gagctagaag acaaaattta cattttattc
    29521 cataaaccaa ctcaagtgat aacaagtgta tctgatgata gaggacgtac agtggttaca
    29581 gattacttta aagatataga agcgcgtatt tatcctgttg gtcgtttaga ctatgatact
    29641 tctggattgt tattacttac aaatgatggt gaatttacta atttaatgac acatccaaga
    29701 tatcaaatta agaagaaata tgtagcaaaa ttaaaaggtt acttaatgag agaagaagtg
    29761 aaagcgctag aaaaaggtat tgaattagaa gatggtatga cgcaaccggc tgaagtgaaa
    29821 gttaagaaac aagataaaga taaaaacaca acattggttg aaattacaat aacagaaggt
    29881 cgtaatcgtc aagtcagaag aatgttcgaa cattttggtc atcaagtgac gaaactgtct
    29941 cgtattgaat atgggccttt aaatgttgtc ggtttgaatg ctggtgaagg acgtgtattg
    30001 acgcctcatg aagtaaaagt gatgcgtcat ttagcagaac atgggaaata attaaataaa
    30061 atatgtattt atcacaaagt ttgagaattt tttcacaaaa ttttaggttt ttaataactg
    30121 tatgcgcttt cctgtgatat aatacttttg taaagaaaag catgtgtggg aggtatgacc
    30181 tgtatgtcga acgaaatact tatcgtagat gatgaggata gaatcagaag gttacttaaa
    30241 atgtatttag aaagagaatc ttttgaaatc catgaggcaa gtaatggcca agaggcttat
    30301 gaacttgcaa tggagaataa ttatgcttgc atactactag atttaatgtt gcctgaaatg
    30361 gatggtatcc aggtggcaac taaattgcgt gaacataaac aaacaccgat tattatgttg
    30421 actgctaaag gtgaagaaac aaaccgtgtt gaaggttttg agtctggtgc agatgattat
    30481 atcgtcaaac cattttcacc aagagaagta gtcttaagag ttaaagcact tctaagaaga
    30541 acgcaatcta caactgtaga acaaagcgaa cctcacgcac gtgatgtgat tgaatttaaa
    30601 catttagaaa tagataatga tgcacatcgc gtacttgctg ataatcaaga agttaatttg
    30661 actcctaaag agtacgaatt attaatatat ttagctaaaa caccaaataa agtatttgac
    30721 cgtgaacaat tattaaaaga agtttggcat tatgaattct atggtgattt aagaacagtt
    30781 gatactcatg ttaaacgact tagagaaaag ttaaatcgtg tgtctagcga agctgcgcat
    30841 atgattcaaa cagtctgggg cgttgggtat aaatttgagg ttaaatctaa tgatgagccg
    30901 gctaaatagt gtcgtaatta aactgtggtt aactattatt ttaatagtga cgacagtttt
    30961 aattttatta agtattgctt taattacctt tatgcaatac tatttcacac aagaaactga
    31021 aaatgccata agagaagatg ctagacgtat aagttcactg gtcgaacaat cacataataa
    31081 agaagaagca ataaaatata gtcaaacatt aattgaaaat cctggtgggt tgatgattat
    31141 aaataataaa catcgtcaat caacggcttc gctttctaat attaaaaagc aaatgttgaa
    31201 tgaagtagtc aacaacgacc attttgacga tgtgtttgat aaaggtaaat ctgttactcg
    31261 aaatgtaacg attaaagaaa agggctcatc tcaaacatat attttgttag gctatccaac
    31321 aaaagcacag aagaatagtc atagcaaata tagtggagtc tttatatata aagacttgaa
    31381 atcaatcgaa gatacaaata atgctattac gattatcact ataattacgg ctgttatttt
    31441 cttaacaatt acaacagtct ttgcgttttt cttatcgtca agaattacaa aacctttaag
    31501 acgtttaaga gaccaagcta cacgtgtatc tgaaggggat tactcttata aaccttctgt
    31561 cacaacgaaa gatgaaattg gtcaattatc acaggcattt aatcagatga gtacagaaat
    31621 cgaagagcat gtcgacgcat tatccacatc taaaaatatt agagacagct taattaactc
    31681 tatggtagaa ggtgtcctag gtattaatga gagtcgacaa attatcttat cgaataagat
    31741 ggcgaatgat attatggaca atattgatga agatgctaaa gctttcttat taagacaaat
    31801 tgaagatact tttaaatcaa aacaaacgga aatgcgtgat ttagaaatga atacacgatt
    31861 ctttgttgtg accacaagct atatcgacaa gattgaacaa ggaggtaaaa gtggtgttgt
    31921 tgtgacagtt cgtgatatga ctaatgagca caatctagat caaatgaaga aagatttcat
    31981 tgctaatgta tcacatgaat tacgtacacc gatatcatta cttcaaggtt atactgaatc
    32041 aattgtagat ggtattgtta cagaaccgga tgaaataaaa gaatcgcttg ccattgtcct
    32101 tgatgaatcg aaacgtttaa atcgtttagt taatgaattg ttaaatgtcg ctcgcatgga
    32161 tgctgaaggg ttatccgtaa ataaagaagt tcagcctatt gcagcgttac tagataagat
    32221 gaaaattaag tatcgtcaac aggctgatga tttaggtcta aatatgactt ttaattattg
    32281 taagaagcgt gtttggactt atgatatgga tcgcatggac caagtactaa cgaacttaat
    32341 tgataatgca tcacgttata cgaaacctgg tgatgaaatt gcaattactt gtgatgaaaa
    32401 tgaaagcgaa gatattttat acattaaaga tacaggtaca ggcattgcac cagaacattt
    32461 acaacaagta tttgatcgtt tttataaagt tgatgcagcg agaacgcgag gtaaacaagg
    32521 taccggttta ggtttgttca tttgtaaaat gattatcgaa gagcatggtg gttccataga
    32581 tgttaaaagc gaattaggga aaggcacaac atttattatt aaactaccaa aaccagaata
    32641 aaattgaata tagttatttc agaacgcatg ttattgatta gagactctaa tttatagcat
    32701 gcgttttttt attgatgtgg ggagttttgt atgtggatta ggatttaggg tttagggttt
    32761 ttgcgaatat caactattaa atatattact aatttatata aaaatataaa gtttgataaa
    32821 gttatttatt tgattataaa aatagggtaa aatatagatg tattgtaata attaaattat
    32881 tcgaggtgtc atatgaaaaa attcattgga tcagttttag ctacgacatt aattttaggg
    32941 ggatgttcca cgatggaaaa tgaatcaagt aaagacacga atacagaaac aaaatcagta
    33001 ccagaagaaa tggaagcttc aaaatatgta ggacaaggct tccaaccgcc tgcagaaaaa
    33061 gatgcgattg aatttgcgaa gaagcataaa gataaaattg ccaaacgagg cgaacaattt
    33121 tttatggata acttcggttt aaaagttaaa gctaccaatg ttgtaggtag tggcgacggt
    33181 gtagaagtat tcgtgcattg tgatgaccac gacatcgtat ttaatgcgag tattccattt
    33241 gacaaatcga taatagagag tgatagctca ttaagaagtg aggataaagg cgatgatatg
    33301 agtactttag taggtacagt gttgagcggc tttgaatata gagctcataa gcaagagttg
    33361 gatagtttaa cagaagtttt gaaagaatat aaaagtaaat ataaatatac tggttataca
    33421 gaaaatgcaa ttatgaaaac gcagaatagt ggttttagaa atgaatatta ttatttgact
    33481 gcaattccat atactttaga tgaatataaa aagtattttc aacccttaat caaagaggat
    33541 gataagactt ttagtgatgg tatgaaaaac tcaaaaaaac aacttaaaga taaatctcgt
    33601 ccatatgttg ttacaacttt atttagcact aaagacaatt acactaaaga taatacaata
    33661 gacgagatga ttgatttttc tgaagtacta aaaaagaaaa agaatattcc tcatgatttg
    33721 aatgtttctc ttcaaattac tgataaatat ataaattcag caagacctag ttacagtaag
    33781 aaagattcaa tagaagtagg agtatttaat catgaaaaaa attaatgata gagatttagc
    33841 agaattaagt ggatactggg catatcaaga tttggacaaa agatatgtaa ttagagtaaa
    33901 taataataaa tatagactaa ttgaagatta ttcagacaat cctaattcta aaagttttgg
    33961 tggtacagat gcaaaagttt ttgaattggt aaatagcaat aatgaattaa caggacaaca
    34021 agctattatc ttccaaggta caaataataa taagagtatc ggaagcaaca atccttttcg
    34081 tgggaaggtt gctgatgatt ggattgaaaa tataaagtta atgaatgata aaaataagtc
    34141 aacttcttta ttagaacaaa ataatgctta tattaaatcg tatgaacaga aactaaaaga
    34201 tgcagataca cttaaaagct ctgatttttt aaggaaatat aagaaaaatc cgtctacgta
    34261 taaaaacaaa actattaagt ctgatggagg caattctcaa gggggagcaa gtgctaacca
    34321 ccaagggatg attaacccga ataaaaatat agtttctaca aaccctgcca tgttaccaaa
    34381 gtcaatatgg gaaaatttca aatctcaaaa ctttgaaaat atgattaatt atcatagtaa
    34441 gtttgatatt ttatcttggc tgcaggatcc atttgccact ccgacactag gaaaacgagt
    34501 gaatttagaa actggtgtgc caacgatgga tggattagta aatagtcatt taggttataa
    34561 aagaaaatta aatccaagat ataatactta taaggattta gcagtttata aaattaaatc
    34621 agttaaagat actatgatta aaaatggtaa aaaagtcaaa aaaactattg aaatcgatat
    34681 aaatatggat gatagaattc caattaatgt gtggacagga gattcaatag cgagaacggg
    34741 taaagggact cctataaaat taaatctaga aaatcttagt gcattaagta atttggtaac
    34801 tggtgaaaca agtaatatgt taaccgattg tgtaaattat ttgaatgaga gctataatat
    34861 ttcacaggtt gaaaatgcta attttggaga aagaaaacat aaacttaaac aagattttaa
    34921 aaagattgtc gaagttgata tccttgaagg aatgagtaga gaattaactt ctttgaaaaa
    34981 ggatgcattt agcgcaattg ataatgtcaa aaaaaatctt gttggaattg ctttcatatt
    35041 ttcatcagtt gcagtcctta tttttgaatt aaatactatt gaagaaatgc ttgaagattt
    35101 agataaaaaa ttacaacgtg gcatagaaag tttacatgat tcattggata acgtaattaa
    35161 agaaatgttt aaaaatttag atcatgattt tgaagatggc gtgacagagg aaatgatgaa
    35221 gcatttaaat gttgtgaata ataatataaa ttttgtaaaa aaacaaaatg atgtatatgg
    35281 agagcagata accgatatta aaaatataat gtctcatcaa gatgcaacgg taatggatgg
    35341 aaacttatct attaattata gcggtgaaca tatgatttcg ggtcaagtac
//
LOCUS       DAKSQE010000016        34093 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.016, whole genome shotgun sequence.
ACCESSION   DAKSQE010000016 DAKSQE010000000
VERSION     DAKSQE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 34093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34093
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.016"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(164..724)
                     /locus_tag="O0V33_000638"
     CDS             complement(164..724)
                     /locus_tag="O0V33_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCX8975306.1"
                     /translation="MNECAFGTKDPVYLDYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAHDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAFGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLKDCPSKPRHN"
     misc_binding    782..1083
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1127..3757
                     /locus_tag="O0V33_000639"
     CDS             1127..3757
                     /locus_tag="O0V33_000639"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCX8975307.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNNKAGKYEGMDRFDCRKQLVEDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNGNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGETIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     gene            3770..5041
                     /locus_tag="O0V33_000640"
     CDS             3770..5041
                     /locus_tag="O0V33_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCX8975308.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPI
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDDIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            5302..6009
                     /locus_tag="O0V33_000641"
     CDS             5302..6009
                     /locus_tag="O0V33_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCX8975309.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCHKRISLTHFFGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSITYPVAFIIISMTTHIFYFLFRSYIG
                     YGDVLLISALSLFFPLQFTIYVIIFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            6006..6692
                     /gene="radC"
                     /locus_tag="O0V33_000642"
     CDS             6006..6692
                     /gene="radC"
                     /locus_tag="O0V33_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCX8975310.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIQVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKETCVFKGTLNSSIVHPREFFSI
                     AVRENANAIIAVHNHPSGDVTPSQEDIITTMRLKECGLILGIDLLDHIIIGDNRFTSL
                     VEAGYFDEND"
     gene            6760..7044
                     /locus_tag="O0V33_000643"
     CDS             6760..7044
                     /locus_tag="O0V33_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975311.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIITGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(7160..7357)
                     /locus_tag="O0V33_000644"
     CDS             complement(7160..7357)
                     /locus_tag="O0V33_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975312.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            7517..7990
                     /locus_tag="O0V33_000645"
     CDS             7517..7990
                     /locus_tag="O0V33_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HCX8975313.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKLEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKPENAYQ"
     gene            8064..8183
                     /locus_tag="O0V33_000646"
     CDS             8064..8183
                     /locus_tag="O0V33_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975314.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNR"
     gene            8383..9225
                     /gene="mreC"
                     /locus_tag="O0V33_000647"
     CDS             8383..9225
                     /gene="mreC"
                     /locus_tag="O0V33_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCX8975315.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            9225..9755
                     /gene="mreD"
                     /locus_tag="O0V33_000648"
     CDS             9225..9755
                     /gene="mreD"
                     /locus_tag="O0V33_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCX8975316.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRSFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     misc_feature    9782..10035
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            10048..10356
                     /gene="rplU"
                     /locus_tag="O0V33_000649"
     CDS             10048..10356
                     /gene="rplU"
                     /locus_tag="O0V33_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCX8975317.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            10362..10682
                     /locus_tag="O0V33_000650"
     CDS             10362..10682
                     /locus_tag="O0V33_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCX8975318.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            10694..10978
                     /gene="rpmA"
                     /locus_tag="O0V33_000651"
     CDS             10694..10978
                     /gene="rpmA"
                     /locus_tag="O0V33_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCX8975319.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
     gene            11465..12757
                     /gene="obgE"
                     /locus_tag="O0V33_000652"
     CDS             11465..12757
                     /gene="obgE"
                     /locus_tag="O0V33_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCX8975320.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLILFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            12770..13225
                     /locus_tag="O0V33_000653"
     CDS             12770..13225
                     /locus_tag="O0V33_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCX8975321.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            13239..13841
                     /gene="ruvA"
                     /locus_tag="O0V33_000654"
     CDS             13239..13841
                     /gene="ruvA"
                     /locus_tag="O0V33_000654"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCX8975322.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIHTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTEQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            13873..14877
                     /gene="ruvB"
                     /locus_tag="O0V33_000655"
     CDS             13873..14877
                     /gene="ruvB"
                     /locus_tag="O0V33_000655"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCX8975323.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERE
                     "
     gene            14879..15904
                     /gene="queA"
                     /locus_tag="O0V33_000656"
     CDS             14879..15904
                     /gene="queA"
                     /locus_tag="O0V33_000656"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCX8975324.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLTEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSTRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            15927..17066
                     /gene="tgt"
                     /locus_tag="O0V33_000657"
     CDS             15927..17066
                     /gene="tgt"
                     /locus_tag="O0V33_000657"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCX8975325.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            17085..17345
                     /gene="yajC"
                     /locus_tag="O0V33_000658"
     CDS             17085..17345
                     /gene="yajC"
                     /locus_tag="O0V33_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCX8975326.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
     gene            17620..19899
                     /gene="secDF"
                     /locus_tag="O0V33_000659"
     CDS             17620..19899
                     /gene="secDF"
                     /locus_tag="O0V33_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HCX8975327.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQYWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKGSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
     gene            20102..22375
                     /gene="recJ"
                     /locus_tag="O0V33_000660"
     CDS             20102..22375
                     /gene="recJ"
                     /locus_tag="O0V33_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCX8975328.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTSTTILVTT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     ALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDDEEEAAFLAEQVEQFNRERKDIVATITEEAMAMA
                     EMKVKNGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     PVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQLIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDVFKKCYKALITKQETNIQKEGMLLCQH
                     LSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQL
                     LYQDFSEIKNWIKSQLS"
     gene            22397..22915
                     /locus_tag="O0V33_000661"
     CDS             22397..22915
                     /locus_tag="O0V33_000661"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCX8975329.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIK
                     DYDVMSLISYDE"
     gene            23343..25532
                     /locus_tag="O0V33_000662"
     CDS             23343..25532
                     /locus_tag="O0V33_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCX8975330.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
     gene            25544..25996
                     /locus_tag="O0V33_000663"
     CDS             25544..25996
                     /locus_tag="O0V33_000663"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCX8975331.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQDSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVNINNDGPVTIIYESQDGKIQ"
     gene            25993..26868
                     /locus_tag="O0V33_000664"
     CDS             25993..26868
                     /locus_tag="O0V33_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCX8975332.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            27329..28591
                     /locus_tag="O0V33_000665"
     CDS             27329..28591
                     /locus_tag="O0V33_000665"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCX8975333.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNY
                     DGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFI
                     VTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELE
                     NNKIDVKNMTTGESETIELDALVEYFKK"
     gene            28607..30373
                     /gene="aspS"
                     /locus_tag="O0V33_000666"
     CDS             28607..30373
                     /gene="aspS"
                     /locus_tag="O0V33_000666"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCX8975334.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFEAENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            30451..30641
                     /gene="ssrS"
                     /locus_tag="O0V33_000667"
     ncRNA           30451..30641
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="O0V33_000667"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            30706..30834
                     /locus_tag="O0V33_000668"
     CDS             30706..30834
                     /locus_tag="O0V33_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975335.1"
                     /translation="MGLIAFNNCFLVEFWKNNQSNNAKHKILSYYVLMYKSVKEVK"
     gene            30834..31607
                     /locus_tag="O0V33_000669"
     CDS             30834..31607
                     /locus_tag="O0V33_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HCX8975336.1"
                     /translation="MKHQFSRNELAIGHQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(31768..33042)
                     /locus_tag="O0V33_000670"
     CDS             complement(31768..33042)
                     /locus_tag="O0V33_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCX8975337.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDEENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSERQLKEIYNNLLKQRP"
     gene            33127..33549
                     /locus_tag="O0V33_000671"
     CDS             33127..33549
                     /locus_tag="O0V33_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCX8975338.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            33649..33831
                     /locus_tag="O0V33_000672"
     CDS             33649..33831
                     /locus_tag="O0V33_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCX8975339.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKATGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            33871..34017
                     /locus_tag="O0V33_000673"
     CDS             33871..34017
                     /locus_tag="O0V33_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975340.1"
                     /translation="MSFIDKAKDAVEKFKNSDNEQVKNVKDKINEYTGSNNEEKKENE
                     DKEK"
BASE COUNT        12197 a         4680 c         6548 g        10668 t
ORIGIN      
        1 gcaaaggttt tacatctatt tttaaattaa gatatctaat attagaagtg cattaacatg
       61 gaactttaac ttgtgatttg aataaatttc tacattaaaa aacgagacag tctattagtt
      121 aaaaatagat gatgtctcgt ttactttatg atgctatagc aaattaattg tgtctaggct
      181 ttgatggaca atcttttaaa tgtgcatcat ataaaccggc tgcctctaaa aacgaaaata
      241 ctgttactgg acctaaaaat ttgaacccgt attgttttaa atctttagat agttgtgttg
      301 cagtatcatc aactgtgata cgatcagaag catgttcata ctgcaaatct ttaggcttac
      361 catttacata agaccataaa aatttactaa aactaccaaa tgcttgttca atttttaaat
      421 acccttgagc ttgattaaca attgcttcta attttttacg atgatgaacg atatttggaa
      481 aagtcattaa gcggtcgata tcatgagcgg tcatttgtgc taccttttct ggttcgaaat
      541 cataaaatgc ttcttcatag gcttctttct tttttaaaat agttaaccaa gatagcccag
      601 catgttgtga ttctaatgct aaaagtttaa acaatgcctt gctatcatag agcggttgtc
      661 cccatacatg atcatgatag tctaagtaga ctggatcttt agttccaaat gcgcattcat
      721 tcattttatt cccacctata cattgactta ttttgtcaat cattatacta taagttaaga
      781 gtttaatata ggatttggga agagtaagtt caaattattt taaagagagc atatgatttg
      841 gtgtgagtat gtaaatgatt tttactgaag gtagcccaca gagtatctta gctgaacgtg
      901 attaatagta ggctaagacg tgtttgagcg ttaatcataa tctagagtgc ttgaagagat
      961 gaagcatttc aaatttcacg atgatagtat tggtatgatt attccaatta tgttaaaaga
     1021 gtgaatttga tattaatcat ccttcttgaa tataggtggt accacggaat atccgtccta
     1081 tttgtatata ggatggatat tttttatttt ttaggaggta tagcaaatgg aaatgaaacc
     1141 aaaatatgat cctcgtgaag ttgaagcggg acgttatgaa gaatgggtga agaatggtta
     1201 ttttaaaccg tcagaagata aatcaaaaga aacatataca attgttatcc cgccaccaaa
     1261 tgtaactggt aaattacatt taggacatgc atgggataca actttacaag atatcattac
     1321 acgtatgaaa cgtatgcaag gatacgatac gttatactta ccaggtatgg atcatgctgg
     1381 tattgcgaca caggcaaagg tagaagctaa attaaatgaa caaggaataa ctagatatga
     1441 tcttggtcgt gaaaagtttt tagaacaagc atgggattgg aaagaagagt atgcgtcatt
     1501 tattcgtgcg caatgggcta aattaggtct aggtttagat tatagtagag aacgttttac
     1561 tttagatgaa ggtttaagta aagcagttaa aaaagttttt gttgatttat acaataaagg
     1621 aattatttat cgtggcgaac gtattataaa ttgggatcct aaagcacgta cagctttatc
     1681 tgatattgaa gtaatacatg aagatgttca aggtgcgttt tatcatttta aatatcctta
     1741 cgctgatggt gaaggtttta ttgaaattgc aactacaaga ccagaaacga tgttaggtga
     1801 tacagcgatt gttgttaacc ctaatgacga acgatacaaa gatgtaatcg gtaaaactgt
     1861 tatattacca atcgtaggac gcgaactgcc tattttagca gatgaatatg ttgatataga
     1921 cttcggttct ggtgctatga aagtgacacc agcacatgac cctaatgatt ttgaaattgg
     1981 tcaaagacat caattagaaa atattatcgt tatggatgaa aatggtaaaa tgaacaacaa
     2041 agctggtaaa tacgaaggta tggaccgttt tgattgtcgt aaacagctag ttgaagattt
     2101 aaaagaacaa gatttagtta tcaagattga agatcatgtt cattctgtag gtcattcaga
     2161 acgatctggc gctgttgttg aaccatattt atcaacgcaa tggtttgtgc gcatggaaga
     2221 cttagcgaaa cgttcattag ataaccaaaa aacagatgat cgtattgatt tttatccgca
     2281 acgtttcgaa catacattta accaatggat ggaaaatatt agagattgga cgatttcaag
     2341 acaattatgg tggggtcatc aaattccggc ttggtatcat aaagaaacag gagaaatata
     2401 tgttggagaa gaagcgccaa ctgatattga aaattggcaa caagatgaag atgtattaga
     2461 tacgtggttc tcaagtgctt tatggccttt ctctacgtta ggttggcctg atttagaaag
     2521 cgaagacttt aaacgatact acccaacaaa tgccttagtt acaggttacg acattatctt
     2581 tttctgggta gcacgtatga tattccaagg cttagaattt acagatcgtc gtccatttaa
     2641 tgatgtgtta ttacacggct tagttcgcgc tgaagacggg cgtaagatga gtaaatcatt
     2701 aggtaatggt gtggatccaa tggatgttat tgacgaatac ggtgctgata gcttgcgtta
     2761 cttcttagca acgggttcat ctccaggaca tgatttaaga tactcaactg aaaaagttga
     2821 gtcagtgtgg aactttatca ataaaatttg gaatggagca cgtttcagtt taatgaatat
     2881 cggtgaagac tttaaagttg aagatatcga tttaagtggt aacttatcat tagcagataa
     2941 atggattcta acacgtttaa atgaaacgat tgcaacagtt actgatctaa gtgacaaata
     3001 tgaattcggc gaagttggac gagcattata taatttcatt tgggatgatt tctgtgattg
     3061 gtacattgaa atgagtaaaa ttccaatgaa tggtaatgat gaagaacaaa aacaagttac
     3121 acgttcagta ttgagttata ctttagacaa tattatgaga atgctacatc cattcatgcc
     3181 atttgtaaca gagaaaatat ggcaaagttt accacatgaa ggtgaaacaa ttgttaaagc
     3241 ttcatggcca gaagtgcgtg aatcattgat ttttgaagaa agtaaacaaa caatgcaaca
     3301 acttgttgaa atcattaaat ctgtaagaca atcacgtgta gaagtaaata cgccattgtc
     3361 taaagaaata cctattttaa ttcaagctaa agataaagaa attgaaacaa ctttatcaca
     3421 aaacaaagat tatttaatca aattctgtaa tcctagtacg ttaaatataa gcactgacgt
     3481 ggaaattcct gagaaagcaa tgacatcagt tgtaattgca ggtaaagtgg tattaccatt
     3541 agaagggcta attgatatgg ataaggaaat cagccgtttg gaaaaagaat tagctaaact
     3601 tcaaagcgaa ttagatagag tagataaaaa gctctctaat gaaaactttg taagtaaagc
     3661 acctgaaaag gttataaatg aagaaaaacg taaaaaacaa gattatcaag aaaaatatga
     3721 tggtgtgaag gcaagaattg aacaattaaa agcataggag ttagtaacaa tgaattacct
     3781 agagagcttg tattggatac acgaaagaac taaatttggc atcaaaccag gtgttaaacg
     3841 tatggaatgg atgctagcac aatttaataa tcctcaaaat aacattaagg gtattcatgt
     3901 aggtggcacg aatggtaaag gctctacagt tgcttacctt agaacagctt tagttgaaaa
     3961 tggttatgaa gtaggtacat ttacgtcgcc gtttattgaa acatttaatg aacgaattag
     4021 tctaaatggt gtgccaatat caaatgacgc tattgtagaa ttagtatcac gtattaaacc
     4081 agttagtgaa atgatggaac gtgaaacaga tttaggtgtt gcaactgaat tcgaaataat
     4141 cacagcgatg atgtttttat attttggtga aatacatcct gttgattttg tcattgttga
     4201 ggctggattg ggtataaaga acgattcgac aaatgtcttt acaccgattt tatcaatctt
     4261 aactagtatc ggtctagacc atacagatat tttaggtggt acttatctag atattgctag
     4321 ggataaaggc gcgattataa agcctaacgt tccagttata tatgctgtta aaaatgaaga
     4381 tgcattaaag tatgttcgtg aacgcgcaat tgaacaacat gcaaagccaa ttgaattaga
     4441 tagagaaatt gttgttgtat cgcaaaatga tgaatttact taccgttata aagattatga
     4501 attagaaaca atcattttaa gtatgttagg tgaacatcag aaacaaaatg ctgcattagc
     4561 cataacagct cttattgaat taaatgaaca aggattaatt gaattagatt tcaataagat
     4621 gatagacggt attgaatcag ttcgttggac tggacgtatt gagcaggtgc atgacaaacc
     4681 tttaatcatt ttggatggcg cacataattc agagagtata gatgctctaa ttgatacaat
     4741 taaacagtac catgataaag aaaaagtaga tattttgttc tcagcaataa acggaaaacc
     4801 gattaacgag atggtcaaac atttaagttt aattgcgcat acattttatg caactgaatt
     4861 tgattttccg aaagcgttac gcaaagaaga aattgtaggt agcattgaaa atgacgatat
     4921 acaattagta gatgactacg tagaatttat aaaaaattat caaggtgata cattagtaat
     4981 taccggtagt ctgtacttca taagtgaagt taaatcaaaa ttagactttg aagcattata
     5041 aaactaaaat agccatgaag aatctattta atcaataggt agcttcatgg ttatttttta
     5101 tgctctaaat cgtatgtgtt tataaggtta atttcttgcc attattaaat ttgccgagca
     5161 gtaatgggct gttaaagtat ccatcaatta tgcttagtaa tattgaaaaa tttaaatgaa
     5221 aaagaggttc aatatttgga gaatagtgta tatttacatt ttggtaataa gtaactataa
     5281 tttacgtaag gaggttgtca tttggtagta ttgttatctt atagttgcag ttgtattttt
     5341 agttttctat atcaatttat atctattgaa gaaacgtcat ttgattattt acatagaaga
     5401 tcgaaatgtg attattgtaa ttcatcactc aaatggtatg aattaatgcc gattattagt
     5461 tttttattat taaaagggag atgtcgaaac tgtcataagc gtatttcgct aacacatttc
     5521 tttggggaaa cctttgcttt aatacctatc gtctttatta agtatgattt cacatacgta
     5581 aatgctacgc tttttataac tacatatgtt tttctgctta tttttactat gaccgatatc
     5641 acttctttaa tgttagattg tcgcttaatt ataatttatt gtattgtttc tctctcgtta
     5701 agtattactt atccagtagc ttttatcatt attagtatga ccacgcatat attctacttt
     5761 ttatttcggt catatattgg ttatggtgac gttttactaa tatctgcact ttctttgttt
     5821 ttccctctcc aattcactat ttatgtcatt atatttacat ttgtcattgc tggtttagtt
     5881 gctttaatta ccatgatatt taagccgatt aaactattac cccttgttcc atttatattt
     5941 atttcctttt ttatcaattc acttttttat aatgatatcc atcaattttt aggaggcgta
     6001 tatttttgaa aattaaagaa atggtaactt cagaaatgcc aagagaacgt ttgttaagcc
     6061 atggtgcaaa aagtctttcg aatacagaat tattagctat attaattaac accggaagaa
     6121 aaggattctc gagtattgac attagtaatg aactgcttaa atctgcatcg aatctgaatg
     6181 aattgaaaaa atcttcaatt aacgatttga tacaagttaa agggattgga ttacaaaaag
     6241 caattacatt aaaagcggca tttgagttag gggaaaggat ggggagaaga gctgaaaata
     6301 atcgtataaa aataacgcaa ccaagtgatg ttgctgatta tatgattcca acaatgaaag
     6361 atttaacaca agaacatttt gtcattttat tgttaaattc aaaaaatgta gtgattaaag
     6421 aaacctgtgt ttttaaaggt acattaaata gttcgattgt acatccacgt gaatttttta
     6481 gtattgcggt gagagaaaat gccaatgcaa tcatcgcagt tcataatcat ccatccggtg
     6541 atgtaacgcc ctcacaagaa gatataataa caacaatgag gttgaaggag tgtggtttga
     6601 ttttaggaat agatttattg gatcatatta taatcggtga taatagattt accagtcttg
     6661 tagaagcggg ttactttgat gaaaatgatt gaagttgata gaattaatga cgtatcttgt
     6721 gtataatacc tacgaagtac tttcattgga ggaaaaatag tgactctatt tattattatc
     6781 ggggttctcg tgccaatggt ttataccatg cagttaaata ttaaaaatga acctgtaaca
     6841 aagcgcaatc tattaataac attagcttta tctacgttag gtattttagt aacggcatta
     6901 gcaggtgtga ttgttacgaa acaagctttt cctttattaa gtgtagcaat aggctcaatt
     6961 attactggaa tcgtttgggg cctattacta agtggtagct acgcgctgat acgattttta
     7021 tctaacgcat ttgggcgtaa ataatagtaa tctgttcatc aagtagtata cgtgcttgaa
     7081 aacaaattaa actcctaatg tggaactagt taaaaaattc taaactagta cattaggagt
     7141 tttgttatgc agaatagatt taattgttaa attgaaagtg tggtaaaaat ccactattta
     7201 tttgaaaaaa atcgagcaac catattaagc cttgatatcc taagtaaata cataataaaa
     7261 caagtccgac ataaattaaa aatcgcaaaa tagaaagtcc aactctaaaa aggaggatga
     7321 ctaatagtgc tattaaaata attgtgagta tactcaatgc tacaaacctc ctcatacgct
     7381 tttaaatcat aacaaaatta taagccattt taaatgaacg tgcatcagtc ctaagatata
     7441 tcatgattaa ggctgaagac tgaggaaaat atacataata aaatatataa ttagatgaag
     7501 acattggagg cgaactatga gattgatatt tagtatgatt aaaaacatta tagcagtaat
     7561 agcaattgtg ataattgttt atatcgcctt gaaatacgca ccttttttaa aagaacaaga
     7621 ttggaatcct gtaaatcacg ataatgatca aatgaatcaa gttacgcaac ccactaatga
     7681 tgcccaacat gtttatgtaa gtggtgagaa atattcatta aaagaaaatg atttaataaa
     7741 aaatgtacca ttaagccaaa ttaaaaatgt atttaaaatg atagataaac tagagtttat
     7801 ggctgtgtct ggaatgaatc gcatggctta taatgatcaa tatattatag gtcaaagagg
     7861 agacgaattt attctttata aatttggaga tgagtcaatg cgtgtttaca atactgaatt
     7921 tgaaatgcaa caagacttaa atgaattagg gcaaaattta caactaaaac ccgaaaatgc
     7981 ttatcaatag gaaattattg cataagaaat atattttagt tatcctgtca ctagaataaa
     8041 tttaaaaggt tggtgtagtt caaatggcag aacaatcaaa acagaaacaa gctaatgaac
     8101 aacaaaaggc ccagaactta ttcgcacgtt ggagacaact tcagaattcg aacagtgaat
     8161 cctcgaacga tacaaacaga taattttaaa atttaaatcg attatttaaa ctactagtga
     8221 ctggaatgtt tgctagtagt tttttgtttt taaaatgcca caaatttttt tacggagttg
     8281 ttcattttca aagtaattcg tagttagtgc tttatttcat aatgggagta atataaaata
     8341 gaaaaggata tacaatattt tgacataata gaggtgttct gggtgcttaa gttttttaaa
     8401 aataacaaat taattgttgt tttatgtgca attatcgttt ttattgcatt aattgggctg
     8461 tccatacgtt cacaatctca atcacctcct gaacaatata taggtgattc tgtgtctttt
     8521 ggacaacgag ttgtgagtta tccagttaat tttgttgctg gtacgattgg ggactttttt
     8581 aaaaaaggag attctaaaga atctaaaaat aagattagcc agttagaatc taagaaccaa
     8641 caattagaag cggaaaatga aaaattaaaa aaagagcttg atttaaaaga tatttcaaaa
     8701 tttgatccta tttctactac ggttttagca agaaatccgg atcagtggat gaatacaatt
     8761 gtaattgata agggatctaa atcaggtata acttcaaata tggctgtgat gacatcacaa
     8821 ggttttgttg gaagagttac taaagttaat aaattttctt cacaagttga tttaatctca
     8881 actaatacac gtgcgggtaa attatctgta aatatacaac acggttctaa aaatatattt
     8941 ggcttaattg atcgttatga tgaaaagaac tcagaacttg taattagtga tattaataat
     9001 agagataata tctcaaaagg tgataaagtc gttacaagtg gattagctga tcaactacca
     9061 agtaatttat atataggaga agtgactaag gttcaaaatg atcaatacgg cttagctaaa
     9121 gaagttaggg ttaagactgg tgcagactta acagatttga gccatgttta tgttgcaaaa
     9181 agagatccta aaacaattcc tgatgatgaa agcagggata aataatgcgt acactgtatt
     9241 attttttgat aggtatttta ctattttata tagatactgc aattgggctt cttataccaa
     9301 tgcacatagg taagtttgaa cttgtatttg taccacacct tacatttatg tatattttaa
     9361 tgatggtagt gtatcgtagc tttggcgtat cattattgct cagcatattt ttaggtgtaa
     9421 tgactgatgt atactttggt agtatttatg gagtgtactt atttggctat atattgtttt
     9481 tagcacttat agatcgattc tttaaaattt tttacaaaga tcattcgatg ttattcatta
     9541 ttatattagc tagcacctta ttattagaag tctatgtggc attaatatac ggtatgttag
     9601 gattcattca atttgatatt attcattttg tagtctttag attattgcca acattaatta
     9661 tgaattttgt actgttgatc atgctttatc cgttgattat aaagttcctt aaaaaaacaa
     9721 acaatgacat tgacatgaaa cgccgtcaat ggtaagatgc aatagttgag tagttataag
     9781 tagctacata caaccgctca aatataggtt caagaacatt tttaatgaag aaagcgattt
     9841 tcccacagag tgcaaacgct tgactcattt gcagaagtta ttgaaagaaa gcgattgtca
     9901 atcaaggcgt tgaaggaaga aacgagaaga gtttatcttt ataaatgact cgagttgcga
     9961 ccgaaacaac gtagagtgca aacgcttgac tcaataacga aatgtcacct atgtatggcg
    10021 tgacttatta tacaggaggt gcaaagtatg tttgctatta ttgaaacagg tggaaaacaa
    10081 atcaaagtag aagaaggtca agaaatcttc gttgaaaaat tagacgtaaa cgaaggagat
    10141 acttttacat ttgataaagt attatttgta ggtggagatt cagttaaagt tggagcgcca
    10201 acagttgaag gtgcaacagt tactgctact gttaataaac aaggtcgcgg taaaaaaatc
    10261 actgtattca catacaaacg tcgtaaaaat tcaaaacgta aaaaaggcca tcgtcaacca
    10321 tacactaaat taacaatcga taaaatcaac gcgtaattat tatgattact gttgatatta
    10381 cagttaatga tgaaggcaaa gtaacagacg ttattatgga tggccatgct gaccatggtg
    10441 aatatggtca tgatatcgtt tgtgctggag cttcagctgt attgtttggt agtgttaatg
    10501 cgattatagg attgacatct gagagaccag atatcaatta tgacgacaat ggtggtcatt
    10561 ttcatataag aagcgttgat acaaataacg atgaagcaca actaattctt caaacaatgc
    10621 ttgtgtcttt acaaactatt gaagaagaat ataatgagaa tattagatta aattataagt
    10681 gaggtgcatt ccgatgttaa aattaaactt acaattcttc gcatctaaaa aaggggtaag
    10741 ttctacaaaa aacggacgtg actctgaatc aaaacgctta ggtgctaaac gtgctgacgg
    10801 tcaattcgta acaggtggtt caattttata tcgccaacgt ggtactaaaa tttaccctgg
    10861 tgaaaatgta ggtcgtggtg gcgatgatac attattcgct aaaatcgacg gcgttgttaa
    10921 attcgaacgt aaaggtcgcg acaaaaaaca agtttctgta tatgcagtag ctgaataatt
    10981 ttgtctagtt aacaccagaa gtgaatcttc tggtgttttt tactttttat aaaacgtttt
    11041 tcattattat tttcattttg ttattaaaaa atgggaatta tagacattat actaaatgtg
    11101 caactgcata agagccccta atcgctaaac tataagggga caaaggaata cagttgcagt
    11161 ataaaaagca aaaagtatcg aatttggtac tcggaaatat cagcaatttg agtaaagtaa
    11221 acttaaaagc aactgcataa gagcccctaa ttatttacaa tatataaggg gctaaaagga
    11281 ttacagttgc agcgtttaaa gaataaactg taccacaatt ggtgttgaga aatataagta
    11341 ttttaaagca aagatttata aaagcaactg cataagagcc cctaattatt tacaatatat
    11401 aaggggctct aatatgctat aattattggg aaaatgaaaa ttatatgtaa aagaggtgag
    11461 atatatgttt gtcgatcaag tcaaaatatc tcttaaagcc ggtgatggtg gtaatggtat
    11521 taccgcatac agaagagaaa aatatgtacc atttggtgga ccagctggcg gtgacggtgg
    11581 taaaggtgct tcagtcgtat ttgaagtgga tgaaggttta agaacgttat tagattttag
    11641 gtatcaacgt cattttaaag caagcaaagg tgaaaatggc caaagtagta atatgcatgg
    11701 taaaaatgcg gaagatttag tattaaaagt tccacctggc acaattatta aaaatgttga
    11761 aacagatgaa gtgttagcag atcttgttga agatggtcaa agagctgtag tagcgaaggg
    11821 cggtcgaggt ggccgaggta attcacgttt tgcaacacct agaaaccctg cacctgactt
    11881 cagtgaaaaa ggtgaaccag gtgaggaatt agatgtatct ttagaattga aattattagc
    11941 tgatgtagga ttagtaggtt tccctagtgt gggtaaatcg actttattat ctatcgtttc
    12001 aaaagctaag cctaaaatag gggcatatca ttttacaacg attaaaccaa atctaggagt
    12061 tgtttcaaca cctgatcaac gaagttttgt tatggcagat ttaccaggtt taattgaagg
    12121 tgcatctgat ggcgttggat taggacatca atttttaaga catgtagaga gaacaaaagt
    12181 tattgttcac atgattgata tgagtggttc tgaaggtaga gagcctattg aagattataa
    12241 agtcattaat caagaattag ccgcgtacga gcaacgttta gaagatagac ctcaaatcgt
    12301 agtagctaac aagatggatt tacctgaatc acaagataat ttaatcttgt ttaaagaaga
    12361 aattggcgaa gatgtgccag ttattccagt ttcaacaata acgcgtgata atattgatca
    12421 attattatat gcaatagcag ataaattaga agaatataaa gatgttgact tcacagttga
    12481 agaagaggag tcagttggca ttaaccgagt attatataaa catacaccgt cacaagataa
    12541 atttacaatt tcaagagatg atgatggtgc ttatgtggta agtggtaatg ctattgaaag
    12601 aatgtttaaa atgactgact ttaacagcga tccagcagta cgtcgatttg ctcgtcaaat
    12661 gcgttcgatg ggtattgatg atgcacttag agaacgtggt tgtaaaaatg gtgatatcgt
    12721 tagaattctt ggcggagaat ttgaattcgt tgaataggag cgtaacatga tggacaataa
    12781 agattataaa aagttttatt taattagaga agatgtcttg cctgaatccg tggttaaaac
    12841 attgaagatt aaagatgcct taaaaagtga tccgacattg tccatttatg atgccgttaa
    12901 acagtttgat ctatctagaa gtgcttttta taaatataga gaaacgatat ttccagtaga
    12961 cgataaaatg cttgaccatc gagaatttac attaatttta tatgtaactg atattgtggg
    13021 tatgttggca cgtgtactag atgttatatc aaagttagaa ctatctgtat taacgattca
    13081 tcaaagtatt ccaatggaag aaaaagcaac aataacatta tcactgaatg ctaaatctaa
    13141 agaaacttca gtagaagatg ttattggcgc tttgagaaat ttagattatg tatcaaaagt
    13201 agaattaatt agtatgagta tgtaaggatg tgcctataat gtacgcgtat gtcaaaggta
    13261 agttaacaca tttatatcct acacacgtag ttgttgaaac tgctggtgtt ggttatgaaa
    13321 ttcaaacacc aaattcttat cgttttcaaa agcatctaga tcatgaagtt ttaattcata
    13381 catctttaat tgttcgtgaa gatgcacaat tattgtatgg atttagtagt gaagaagaga
    13441 aagatatgtt cttgagttta attaaagtca ctggtattgg tccgaaatca gctttagcta
    13501 ttttagcgac aagtacacct aatgaagtaa aacgtgccat tgaaaatgaa aatgatacgt
    13561 atttaactaa attcccagga attggtaaga aaacggcaag acagattgtc ttagatttaa
    13621 aaggtaaagt gaaaatcact gaagaagata gcgattcatt attacaagta gacgctacat
    13681 cgacggagca agatcaattc gtgcaagaag caatgttagc gttagaagca ttaggttatt
    13741 ctaaacgaga gcttgcaaaa gttgagaaaa cgttaaataa aaataaatat gactcagttg
    13801 atgaagctgt taaggcaggt cttcaattag ttgtatctta attttaaata gattaatagg
    13861 ggaagtgttg tcatgaatga gcgtatggtt gatcaatcaa tgcatagtga agaaactgat
    13921 ttcgaattgt cgcttagacc tacgagatta cgacaatata ttggtcaaaa ttcaataaaa
    13981 agtaatttag aagtatttat taaagcggct aaacttcgtc atgaaccatt agatcatgta
    14041 ttgctttttg gcccccctgg attaggtaag acaacattat ctaatatcat tgccaatgaa
    14101 atggaagtta atatacgtac agtatcaggg ccttcattag aaagacctgg tgatttggct
    14161 gcaattttat cagggcttca acctggagat gttttgttta ttgatgaaat acacagactg
    14221 agtagtgttg ttgaagaagt gctataccct gcaatggaag atttcttttt agatattatc
    14281 attggtaaag gcgatgaggc tagaagtatc cgtatcgact tacctccatt cactttggta
    14341 ggtgcaacaa cacgagctgg tagcttaaca ggtccactaa gggatcgatt tggtgtgcac
    14401 ttaagattag aatattataa tgaatcagat ttaaaagaaa tcattattag aacagctgag
    14461 gttttaggca caggtattga tgaagaaagt gctattgaac ttgctaaacg ttctagaggg
    14521 actccaagag tagccaatcg actattgaaa cgggtaagag acttccagca agtgaatgaa
    14581 gatgaacaaa tatacattga aacaacaaag cacgcattag gtttacttca agttgatcaa
    14641 cacggactag attacattga tcataaaatg atgaactgta ttattaagca gtataatggt
    14701 ggacctgttg gtttagatac gattgccgta acaattggcg aagaacgtat tacaattgag
    14761 gacgtttatg agccatttct tattcagaaa ggctttttag aacgtacgcc gcgtggcaga
    14821 aaagcaacac cattagctta tgaacatttt gcaaagtcga atgaggagag agaataacgt
    14881 gaatattgaa gaatttgact atgacttacc agaatcatta attgctcaaa cgcctttaaa
    14941 agatcgtgat catagtcgtt tattagtcat ggatagagaa actggtgaaa tgaaacattt
    15001 acattttaaa gatatcattg agtattttag acctggtgat acattagtgc ttaacgatac
    15061 gcgagtaatg ccagctagac tttttggttt aaaagaagaa actggtgcaa aagttgaaat
    15121 gttaatgtta actcaaattg aaggtaatga ttgggaagtc ttactgaaac cagctaagcg
    15181 tattaaagtt ggtaataaat tgaattttgg taatggcaaa attatagctg aatgcataaa
    15241 agaaatggat caaggtggac gtatcatgcg tttacattat gaaggtattt tacaagaaag
    15301 attagatgaa ttaggggaaa tgccactgcc accatacatc aaagaacgtt tagatgatcc
    15361 agatcgttat caaacagttt acgctaaaga aagtggttca gcggcagcac caacagctgg
    15421 attacatttt actgatgagt tattaactga aattaaaaat aaaggtgtta atatcgcatt
    15481 tgttacatta catgttggat taggtacgtt tagaccggtg agcgtcgacg atgtgaatga
    15541 ccacgaaatg catagtgaat attatcaaat gacacaagaa acagctgatt tattaaatga
    15601 tactaagtcc aaaggacatc gcattatatc agttggtaca acttcaacac gtacacttga
    15661 aacaattcga cgcgatcatg ataaatttgt tgaaacgagt ggctggacta atatatttat
    15721 ttatccagga tttgatttta aagctattga tggccagatt actaattttc atttaccaaa
    15781 atcaacatta gttatgctag tatcagcgtt tagtactcgt gaaaatgttc tgaatgctta
    15841 taaaacggca gtaaatttag aatatagatt ctttagtttt ggcgatgcaa tgttaattat
    15901 ataaaaagaa tgtgaggatt ttgaatatgc ctgcagtaac atacgaacac attaaaactt
    15961 gtaaacaatc aggtgcgcgt ttaggtatcg tgcacacacc acacggttca tttgaaacac
    16021 ctatgtttat gccagttggt actaaagcaa ccgttaaaac aatgagtcca gaagagttaa
    16081 gacaaattga agcaaaaatc attttgggca acacatatca tttgtggtta caacccggaa
    16141 atgatattat caaacacgct gggggattac ataaattcat gaattgggat ggtccgattc
    16201 taacagattc aggcggtttc caagtgttta gtttaagtaa tttacgtaaa attacagaag
    16261 aaggcgtgga atttagacat catactaatg ggtctaaatt atttttgagt cctgagaaat
    16321 caatgcaaat tcaaaatgat ttaggttctg atattatgat ggcatttgat gaatgtccac
    16381 cgatgcctgc tgaatatgat tatgtaaaaa aatctattga acgtacaacg cgttgggcga
    16441 aaagatgtct agatgcacac caaagacctg aagatcaagc attgttcggc attatacaag
    16501 gtggcgaata tgaagattta agagaacaaa gtgcaaagga tttagtagaa ttagattttc
    16561 ctggttatgc aatcggaggt ttgtcagttg gtgaacctaa accggttatg tataaaatgg
    16621 ttgaacatac agagcagttt atgcctaaag ataaaccaag atatttaatg ggtgtaggat
    16681 ctccagatgc gttaatcgaa tgtagtattc gcggcatgga tatgtttgat tgtgtcttac
    16741 cgacacgtat tgccagaaat ggtacttgta tgacatcgca aggtcgttta gttattaaaa
    16801 atgcaaaatt tgcagatgat ttaagaccgt tagatgagaa ttgtgactgt tatacatgtc
    16861 aaaactactc aagagcgtat atacgtcatt taatcaaggc agaggaaact tttggtattc
    16921 gtcttactac tattcataat ttacattttc tgctaaaatt aatggaagat ataagacaag
    16981 ccattcgaga agatcgtctt ttagatttca aagaagaatt cttcgagcaa tatggattaa
    17041 atgttgagaa cccaaaaaac ttttaagcaa gaggagcgta taaaatgcaa ttttcattac
    17101 ttatatatat agtcgtaatt tttgcggtta tgtatttctt gatgatcaga ccacaacaaa
    17161 aacgtgcgaa acagcatcgt gagttgatta ataacattca atctggtcaa agaattacaa
    17221 ctattggtgg tattaaaggt actgttaaag cagtagatga aacaactgtt gttattacag
    17281 ttaatggtca tggtactgaa ttaactttcg aaaaacctgc tattaaacaa gttgaccctt
    17341 cataacaaga agtggtatta aaaaggatga aacgaatagc gtttcatcct ttttaagtta
    17401 taagtagtat gtagaatagc ttatttttat tccaaaaaaa gaaattgtga tacataataa
    17461 ttgttaaatt taaattaaat atatctatga ctatttttac tccctgctct agtatgttaa
    17521 tatgtataag gctaataaat tttgtaagaa agatttaact tgcgttataa aatagtattc
    17581 aagtattcta aatacatacg taaataatga ggtgttcatg tgaagaaaag tagtagaata
    17641 attgcgttct tgcttttagt cgtattgttg tttgccggaa tggctgcaac ttataaaagt
    17701 gttataaaga atgtcaattt aggacttgat ttgcaaggtg gatttgaggt gctttatcaa
    17761 gtcgaccctt taaataaagg cgacaaaata gataaaaaag ccttacaatc aacagcgcaa
    17821 acattagaaa atcgtgttaa cgttttaggt gtttccgaac ctaaaattca agttgaagaa
    17881 cctaatagaa ttagagttca acttgctggt gtaacagacc agaatgaagc acgtaaaatt
    17941 ttatcttctc aagctaattt aacaattcgc gatgcagaag ataaagtaaa attaagcggt
    18001 tctgatatta aacagggatc tgccaaacaa gaatttaaac aggaaacaaa ccagccaaca
    18061 gttactttca aagtaaaaga caaaaacaaa tttaaaaaag taacggaaga aatctctaag
    18121 aaacgtgata atgtcatggt tgtttggtta gatttcaaaa aaggtgacag ctataaaaaa
    18181 gaagcacaaa agaaaaaccc taaatttatt tctgcagcaa gtgttgatca accaatcaat
    18241 tctgatagcg tagaaatctc tggtggattt aaaggacaag aaggcgttaa aaaagcaaaa
    18301 caaatcgccg aattgttaaa tgcaggttct ttaccagttg acttgaaaga aatttattca
    18361 aactcagtag gtgcccaatt tggtcaagat gcattagata aaactgtgtt tgcatcgttt
    18421 attggtgtag cattaattta tttattcatg cttggattct accgattacc tggtttagta
    18481 gcgattattg cattgacaac ttatatctat ctaacgttag tagcatttaa tttcatttcc
    18541 ggggttctaa ctttaccagg attagcggcg ttggtattag gtgtaggtat ggctgtagat
    18601 gccaatatta tcatgtatga gcgtattaag gatgaacttc gaataggtag aacgataaag
    18661 caagcctttt ctaaagcaaa caaaagttcg ttcctaacaa tttttgattc taacttaaca
    18721 acagttatcg ccgcagcagt attattcttc ttcggtgaaa gttcagttaa aggtttcgcg
    18781 acaatgttat tattaggtat tctaatgatc tttgttacag ccgtgttctt atcaagattc
    18841 ttattatcat tacttgtttc atcaaatata ttcaaaaatc aatattggtt atttggtgtt
    18901 aaaaagaata aacgacatga tattaatgaa ggtgtagatg ttcatgacct taaaacttca
    18961 ttcgagaaat ggaattttgt taaattagct aagccattaa ttggagtaag tattttaatt
    19021 gtagttgtcg gtttagttat tctttatatc ttcaagttaa acctaggaat tgatttctca
    19081 agtggtacac gtgtagattt ccaatctaaa caagcaatta ctcaacaaaa ggttgagcaa
    19141 gttgtaaaag gtagtggact taaagcggat caaattcaaa tcaatggtaa agataataaa
    19201 gtagcaactg ttcaatttaa agacgattta acgcgtgctc aagataataa attgagtgat
    19261 aatattaaat cgaaattcgg tgacacacct caaattaaca ctgtatcacc aattattgga
    19321 caggaattag caaaaaatgc aatgcttgca ttaatctatg catcgatagg tatcatcatc
    19381 tatgtatcat tacgatttga atggcgcatg ggtctttcat ctgtattggc attattacat
    19441 gatgtattca ttatagtagc gattttcagt ttatttagaa ttgaagtaga tttaacattt
    19501 atcgccgctg tattaacaat tgtcggttat tcaattaatg atacaatcgt aacgtttgac
    19561 cgtgtacgtg aaaacttaca aaaggttaaa gtgattacta caacagaaca aattgatgat
    19621 atcgttaata gatcaattag acagacaatg acacgttcaa ttaatacagt attaacagtt
    19681 attgtagtag tagttgctat actattcttc ggtgctccta cgatattcaa ctttacttta
    19741 gcattattta tcggattgat ttctggtgta ttctcttcaa tcttcattgc cgttccgctt
    19801 tggggaataa tgaaaaaacg tcagttgaaa aaatcgccga aacacaaatt agttgtatat
    19861 aaagaaaaga aatcgaacga tgaaaagatt ttagtttaaa atgaattaag cggtatgtga
    19921 aacattaaag agttcacata ccgcttttta ctttgtttaa atttaacaaa atatatataa
    19981 ataagttccc gcattcattt ttaaaaaatg gtataattaa agtcttgatt atttagataa
    20041 gtgcttttta tttaaagtaa ttttagatat aatgtctttt ggaaatgagg cgtaaatata
    20101 tatgattaaa ccgaagtata agtggaaatt aactaaacca gctgaataca taagtgatga
    20161 attaacatca aaattaaagt taacgccaat cgtaaaaaaa atattagaaa gtaaatcgat
    20221 tattgatgaa caagcaattg aatccatcat ttcagataca gatatcaatc atgacgcatt
    20281 gcaattgagc gatatgacta agactattga aagaattaaa agagcgatag cgaatgatga
    20341 gaaaatccta gtttatggtg attacgatgc cgacggtgtt acctcaacta caattttagt
    20401 gacaacatta caacttttag gtgcacaagt aggatggcat attcctaacc gctttaccga
    20461 aggatatgga ccaaacgagc tagcatttag aaatgctcat gatgaaggca taacattaat
    20521 cataactgtt gataatggca tacaaggtca taatgaaatt aaaatggttc aagatttagg
    20581 agttgatgtc attgtaactg atcatcatga aattggtagt acattaccag aagcatatgc
    20641 aatcgtacat ccaatgcatc catcgtttaa ttatccgttc caacaattgt gtggtgcagg
    20701 tgttgcgtac aaattagcac aagcattgat tgaaaatgta cctgattatt ttaaagcatt
    20761 agttgcgata ggtaccattg cggatttagt atcattaact gatgaaaatc gatcattagt
    20821 gaaacaagga ttaaaagtct taaatgatca atgtccgacc tctgttaaag cattgctaaa
    20881 agaagcggga tacaatgaca atattgatga agaaacaatt ggttttatta ttggacctag
    20941 acttaatgct gtaggtcgtt tagatgacgc atcattagct tgcgaattgt tgatgactga
    21001 cgacgaagaa gaagctgcat ttttagcaga acaagttgaa caatttaacc gcgaaagaaa
    21061 agatattgta gcaactatca cagaagaagc aatggcaatg gcagaaatga aagttaaaaa
    21121 tggagattta tttttacttt tagcaaaaga aaattggcat gaaggtgtct taggtattgt
    21181 tgcttcgaaa attgtcgaaa catttgcttt gccaacatta attttaaata ttgataggga
    21241 gcaaaatcat gcaaaaggtt ccgccagatc aattgatcaa gtgtcgatgt ttgaaatact
    21301 atcagcacac caagagttaa tcgctaaatt tggcggccat catatggctg caggtatgac
    21361 gatggatatt gaaaatatag aatcgttagc tgaaggttta aataaatgga tgaaagaatt
    21421 atctaaaaca acttcgttag atcccgtaaa gccagtagat gtattattaa cagaaaatga
    21481 tataacgatt aaaaatattc gtgatatgaa tcgtttaaga cccttcggaa cagatttttc
    21541 tagacctatt tttgaaatgg atgatttatc agtttcttca gttaaggcga taggtcaaca
    21601 aaaaaatcat ctgaaattga cacttggtga aagtaatatc gccgcgctat tttggcaaaa
    21661 tggacactta gaacctgaat tacaagatga acagccaatt aatatattag gatctgttca
    21721 aatcaacgaa tggaatggta atcaatcacc gcaattaatt attcaagata ttgcgatgaa
    21781 cgaacagcaa atattagatt atagaagtaa gcgaaaaagt ttacctttta cagaaaatga
    21841 tgaaaatatt gtcgtgctta ttcatcctaa aagtgataaa gtaaatgcga atgaatatta
    21901 ttatggtgaa gaaattaaac aacaaactga taaagtagta ttaagagatt tacctacgtc
    21961 aatggaagat ttgtctaatt ccctgcaaca actgcaattt tctcaacttt atatagtttt
    22021 gcaacataat cattcgattt acttcgatgg tatacctaat atggatgttt ttaaaaagtg
    22081 ttataaagca ttaataacta aacaagaaac aaatatccag aaagagggta tgttattgtg
    22141 tcaacattta agtgtgaaac cagatacact taaattcatg ttgaaagttt tcttagactt
    22201 aaaatttgta acacaagaag atggtttaat tcgaatcaat caacaacctg ataaaagatc
    22261 gattgattcc agcaaagtat atcaattaag acaacaacgt atggatgttg aaaagcaatt
    22321 attatatcaa gatttttcag aaataaaaaa ttggataaag tcacaattgt cgtgagcaat
    22381 ttaggaggaa atattaatgg atttaaagca atacgtatca gaagttcaag attggccgaa
    22441 accaggtgtt agtttcaagg atattactac aattatggat aatggtgaag catatggcta
    22501 tgcaacagat aaaattgtag aatacgcaaa agacagagat gttgatatcg ttgtaggacc
    22561 tgaagcgcgt ggctttatca ttggctgtcc tgtagcttat tcaatgggga ttggctttgc
    22621 acctgttaga aaagaaggga aattacctcg tgaagtcatt cgttatgagt atgacctaga
    22681 atatggtaca aatgttttaa caatgcacaa agatgcaatt aaaccaggtc aacgtgtgtt
    22741 aattacagat gatttattag ctactggtgg tacgattgaa gcagcaataa aattagtcga
    22801 aaaattaggt ggtatcgtag taggtattgc atttataatt gaattgaaat atttaaatgg
    22861 tattgaaaaa attaaagatt acgatgttat gagtttaatc tcatacgacg aataaaaaat
    22921 aatataattt tatcaaataa aatccttcat caaatgaata agaacgaatg acttaattaa
    22981 aaaagttgtt taagttttct taacatgaga tgttaggatt ttttatttac tgaaaatgtt
    23041 agatgattga gcattatacc ttaataacaa cgtttattta tttcataaat tgtagtatca
    23101 tagaactaat atttaaaaaa tgaaacagta gatttaggtc gaatttttgt aaaagtttta
    23161 aaagtaggaa tagtatacaa attaaactcg ctcaagtaaa attaatatta cgattaatga
    23221 cgacaggata aatatttatc gtcgacggac gtatgattgg tgtgggacaa atactattca
    23281 acaagagcac ctaaatcatt gtttaaggcg aagtaataaa tatgaatggg gtgtatcata
    23341 taatgaacaa cgaatatcca tatagtgcag acgaagttct tcacaaagca aaatcatatt
    23401 tgtcagcaga tgaatatgag tatgttttaa aaagctatca tattgcttat gaagcacata
    23461 aaggtcagtt ccgaaaaaac ggattaccat acattatgca tcctatacaa gttgcaggta
    23521 ttttaacaga aatgcgatta gacggaccga cgattgtcgc aggttttttg catgatgtaa
    23581 ttgaagatac accgtataca tttgaagatg taaaagaaat gttcaatgaa gaagttgctc
    23641 gaattgttga tggtgtgacg aagcttaaaa aagtaaaata ccgatcaaaa gaagaacaac
    23701 aagctgaaaa tcatcgcaag ttatttattg cgattgccaa agatgtacgc gtaattttgg
    23761 tgaaattagc agacagatta cataatatgc gtaccttgaa agccatgccg cgcgaaaaac
    23821 aaattagaat ttctcgagaa acattagaaa tttatgcacc tttagcacat cgtcttggta
    23881 ttaatacaat caaatgggaa ctagaagata cggctctgcg ttatattgat aatgtacaat
    23941 attttagaat agtcaattta atgaagaaga aacgtagtga acgggaagcg tatatcgaaa
    24001 cggctattga tagaatacgt accgaaatgg accgaatgaa tatcgaaggc gatataaatg
    24061 gtagacctaa acatatttac agtatttatc ggaaaatgat gaagcagaaa aagcaatttg
    24121 atcaaatttt tgatttgttg gcgatacgtg ttattgtcaa ttctattaat gattgttatg
    24181 cgatacttgg gttggtgcat acgttatgga aaccgatgcc aggacgtttt aaagattata
    24241 ttgcaatgcc taaacaaaat ttgtatcagt cattgcatac tacagtagta ggtccaaatg
    24301 gagacccgct cgaaatccaa atacgaacgt ttgatatgca cgaaattgct gaacatggtg
    24361 ttgcagcaca ctgggcttac aaagaaggta aaaaagtaag tgaaaaagat caaacttatc
    24421 aaaataagtt aaattggtta aaagagttag ctgaagcgga tcatacttcg tctgacgctc
    24481 aagaatttat ggaaacctta aaatatgact tacagagtga caaagtatat gcatttaccc
    24541 cagcgagtga tgttattgag ttgccatatg gtgctgtgcc gattgatttt gcttatgcga
    24601 ttcacagtga agtaggtaat aagatgattg gtgccaaggt gaatggcaaa attgtaccaa
    24661 ttgactatat tttacaaaca ggcgatattg ttgaaatacg tactagtaaa cattcatatg
    24721 gaccaagtcg tgattggttg aaaattgtta aatcgtctag tgccaaaggt aaaattaaaa
    24781 gtttcttcaa aaaacaagat cgttcatcta acattgaaaa aggacgaatg atggttgaag
    24841 ctgaaataaa agagcaagga tttagagtcg aagatatttt gacagagaaa aatattcagg
    24901 ttgttaatga aaaatataac tttgcaaatg aagatgattt attcgcagct gtaggatttg
    24961 gcggcgtgac atccttacag attgttaata aattaactga aagacaacgt attttagata
    25021 agcaacgtgc tttaaatgaa gcacaagaag ttacgaaatc attgcctatt aaagacaaca
    25081 tcattactga tagtggtgtc tatgtagaag gtttagaaaa tgtacttatc aagttgtcaa
    25141 aatgttgtaa tcctatacca ggtgatgata ttgtaggtta tatcaccaaa ggtcatggta
    25201 ttaaagtaca tcgcactgat tgcccaaata ttaagaacga aactgaacga ctaattaatg
    25261 ttgaatgggt aaaatcaaaa gacgcaactc aaaaatatca ggttgattta gaggtaactg
    25321 cgtatgaccg aaatggcttg ttaaatgagg tactacaagc tgttagctcg acagccggca
    25381 atttaattaa agtttcaggt cgttcagata ttgataaaaa tgcaataata aatattagtg
    25441 tcatggtgaa aaacgtgaat gatgtttatc gtgtggtaga aaagatcaaa caacttggtg
    25501 atgtttatac agtaacaaga gtttggaact agaggtgcaa aatatgaaag tagttgtaca
    25561 aagagttaaa gaagcatcgg tgacgaatga tacattaaat aatcaaatca aaaaaggata
    25621 ttgtttatta gtcggtatcg gtcaggactc tacagagcaa gatgcagatg taattgcaaa
    25681 gaaaattgct aatgcaagat tatttgaaga tgacaataat aaattaaact ttaatatcca
    25741 acaaatgaat ggtgaaatat tatcagtttc acaatttact ctctatgcag atgtaaaaaa
    25801 aggtaaccgt ccaggtttct caaattctaa aaatcctgat caagcagtaa aaatatatga
    25861 gtattttaat gatgcgctac gagcgtatgg tctaactgtg aaaacaggtg aatttggaac
    25921 acacatgaat gttaatataa ataatgatgg tccagtcact attatttatg aaagtcagga
    25981 cggcaaaatt caatgaaaaa aatagaggca tggttatcta aaaagggtct taaaaataaa
    26041 cgtactctaa tagtagtgat tgcctttgtc ttatttatca tctttttatt tttattgctg
    26101 aatagcaata gtgaagatag tgggaacatt acgataactg aaaatgctga attacgtaca
    26161 ggtccaaacg ctgcgtatcc agtcatttac aaagttgaaa aaggtgacca ttttaaaaag
    26221 attggtaaag taggtaaatg gattgaagtt gaagatacat ccagtaatga aaaaggttgg
    26281 atagctggat ggcacacaaa tttagatatt gtcgcggata atacgaagga gaaaaatcct
    26341 ttgcaaggta aaacaatagt gcttgatcct ggtcatggag gtagtgacca gggtgcttca
    26401 agcaatacta aatataaaag tttagaaaaa gattatacgt tgaaaacagc aaaagaattg
    26461 cagcgtactt tagaaaaaga aggcgcaact gttaagatga caagaacaga cgatacatat
    26521 gtttcactag aaaatcgtga tatcaaaggc gatgcatatt tgagtataca taatgatgcc
    26581 ttagaatcat ctaatgcaaa tggaatgaca gtttattggt atcatgataa tcaaagagct
    26641 ttagcagata cgttagacgc tacgattcag aagaaaggtc tactttctaa tcgcggttca
    26701 agacaagaaa attatcaagt gttaagacaa acaaaagttc ctgctgtttt attagaatta
    26761 ggttatatta gtaacccaac tgatgaaacg atgattaaag atcaattaca tagacaaatt
    26821 ttagaacaag caattgttga tggccttaaa atttattttt ctgcgtaggg cttgcaaaaa
    26881 tatgtgaaag tagttatcat tgatattgaa ttttataact aaaaccgtta gtattcttga
    26941 aatggtaaat gaaataggta gcaatctaac taagattgtg taggaatata atccatagac
    27001 tgaaagatta tgctgagtag tttatataca ttgaacacaa gaagaggtgc tttatgaaaa
    27061 gtaaagccgt taaacgtacg ttaaacgttt tgagtgggtt tattaaatga acgcttataa
    27121 aaagtaatga tgattacaat taggcatgtt ttttaaacca atgagggtgg caacacggat
    27181 attcgtccct tgtgcagttt caggctgtgc aagggctttt tcttttgctt ttaaaattat
    27241 gatttatcgt gcaaagttaa gtggtcgtat atagttttag ttttaaaaag gtaattaaat
    27301 aaaatagttt gccgagggag atatcaaaat gattaaaata cctagaggga cgcaggatat
    27361 tttacctgaa gattcaaaga aatggcgtta cattgaaaat caattagatg aattaatgac
    27421 attttacaat tataaagaaa taagaacacc aatttttgaa agtacagatc tttttgcaag
    27481 aggtgttggt gattcaaccg atgtcgtaca aaaagaaatg tatacattta aagataaagg
    27541 cgatagaagt attacattaa gacctgaggg aacagctgca gttgtgcgtt catatattga
    27601 acataaaatg caaggtaatc caaaccaacc aattaaactt tattacaatg gaccgatgtt
    27661 tagatatgaa cgtaagcaaa aagggcgcta tcgtcaattt aatcaatttg gtgtagaagc
    27721 tattggtgct gaaaatccta gtgtagatgc agaagtatta gctatggtta tgcatattta
    27781 tcaatcattt ggattaaaac atttaaagct tgttattaat agtgtagggg atatggcgtc
    27841 tcgaaaagaa tataacgaag cgttagtgaa acactttgaa ccagtaattc atgaattttg
    27901 ttcagattgt caatcacgtt tgcatacaaa tccgatgcga attttggatt gtaaagttga
    27961 ccgtgataaa gaagcgatta agactgcacc tagaatcact gatttcttaa atgaggaatc
    28021 taaggcatat tatgaacaag taaaagctta tttagatgat ttaggtattc catatattga
    28081 agatcctaac ttagttcgtg gattggatta ttatacacat acagcatttg aattaatgat
    28141 ggataaccct aactatgatg gtgccattac aactctttgt ggtggtggcc gttataatgg
    28201 tttattagaa ttgctagatg gtccaagtga aacaggtatt ggttttgcgc taagtataga
    28261 gcgattattg cttgcacttg aagaagaagg tatcgaatta gatattgaag aaaacttaga
    28321 tttattcatt gttacaatgg gtgatcaagc agatcgatat gctgtgaagt tattaaatca
    28381 tttgagacat aatggtatta aagcagataa agactactta cagcgtaaaa ttaaaggaca
    28441 aatgaaacaa gcagaccgtt taggtgccaa gtttacaatc gttattggtg atcaagaatt
    28501 agaaaataat aaaatcgatg ttaaaaatat gacaactggt gaatctgaaa caattgaatt
    28561 agacgcatta gtcgaatatt ttaagaagta gagagggcgt taaaatatga gtaagagaac
    28621 aacttattgt ggattagtta ctgaagcatt tttaggacaa gaaattacat taaaaggatg
    28681 ggttaacaat cgtcgtgacc taggtggatt gattttcgtt gatttaagag atagagaagg
    28741 aattgtacaa gttgtgttta atcctgcatt ttcagaagag gcattgaaaa ttgctgaaac
    28801 agtacgttct gaatatgttg tagaagttca aggtacagtt acgaagcgtg accctgaaac
    28861 tgttaatcct aaaattaaaa ctggccaagt tgaagtacaa gttacaaata ttaaagtgat
    28921 taataaatct gagacaccac cattttctat aaatgaagaa aatgttaacg ttgatgaaaa
    28981 tattcgatta aaataccgtt atttagattt acgtcgtcaa gagttagcgc aaacatttaa
    29041 aatgagacat caaattacac gttctattcg tcaatatttg gatgatgaag ggttctttga
    29101 catcgaaaca ccagtactaa cgaagtcaac acctgagggt gcacgtgact atttagtacc
    29161 atctcgtgtc catgatggtg aattttatgc attaccacaa tcgccacaat tatttaagca
    29221 attattgatg attagcggat ttgacaaata ctaccaaatt gtaaaatgct tccgtgacga
    29281 agatttacgt gcagatcgtc aacctgaatt cacacaagtc gatattgaaa tgagttttgt
    29341 agatcaagaa gatgtgatgc aaatgggtga agaaatgctt aaaaaagttg ttaaagaagt
    29401 taaaggcgtt gaaattaatg gcgctttccc acgcatgaca tataaagaag cgatgcgtcg
    29461 ctatggttct gataaaccag atacgcgttt tgaaatggaa ttaattgacg tttctcaatt
    29521 aggacgtgat atggacttta aagtatttaa agatactgtt gaaaatgatg gtgaaattaa
    29581 agcaattgtc gctaaaggtg cagctgaaca atatacacgt aaagatatgg atgctttaac
    29641 agaatttgta aacatctatg gtgctaaagg attagcatgg gttaaagttg tggaagatgg
    29701 tttgacaggt ccaattggac gtttctttga ggcagaaaat gttgaaacat tacttacatt
    29761 aactggtgct gaagctggtg acttagtaat gtttgttgca gacaaaccaa atgtcgttgc
    29821 acaaagttta ggtgcattac gtgtcaaatt agctaaagaa ttaggtttaa tcgatgaaac
    29881 aaaattaaac ttcttatggg tgacagattg gccattatta gaatatgatg aagatgcgaa
    29941 acgttacgtt gcagcacatc atccatttac atctccaaaa gaagccgata ttgctaagct
    30001 tggcactgcg ccagaagaag ctgaggcaaa tgcttatgac atagtattaa atggttatga
    30061 attaggtggc ggttcaatca gaattcatga cggtgagtta caagaaaaaa tgttcgaagt
    30121 tcttggattt actaaagaac aagcacagga acaattcggc ttcttactag atgcatttaa
    30181 atatggtgca ccacctcatg gcggtattgc attaggatta gaccgattag tcatgttatt
    30241 gactaataga acaaacttga gagatacaat tgcattccct aaaacagcat ctgctacatg
    30301 tttattaacg aatgcgcctg gtgaagtttc tgataaacaa ttagaagaac tttctttgcg
    30361 aattcgtcac taaattttaa aaaatcaata aactttggtt tgttataatt tcattaattt
    30421 taattatgtg gtaatataac atcataagat agtcatctga gttgttcgta agtttgcttt
    30481 ttaatttggg cctaacactc ttcgatcaag ggagcccaat gggttttctt gcagcgtaca
    30541 cgccttatat gaaggacgta caaaacaaga aacagggcac ccacctgtat ataacaggcc
    30601 gaatgatcaa gctattataa ctacggcaat acggactcta tcgatacgca agactttgtc
    30661 ttgcgtattt ttatgctttt ctttaagttt ttaataaaga attagttggg tttaatagcg
    30721 tttaataatt gtttcctcgt tgaattttgg aaaaataatc aaagcaataa tgctaaacat
    30781 aaaattttaa gttactatgt acttatgtat aaatcagtga aagaggtaaa ataatgaaac
    30841 atcaattttc aagaaatgaa ctagcgattg gacatcaagg tttagattta ttgaagcaac
    30901 aaactgttgt catacttggc gttggaggtg ttggttcatt tgctgcggaa gcattagcaa
    30961 gaacgaatat tggtcatatt atattaatag ataaagatga tgttgatatt acaaatgtaa
    31021 acagacaatt acacgcttta actacaacgg taggacaaag taaagtttct ttgatggaag
    31081 aacgtattaa attaattaat ccagattgta aagtcacgcc tttacatatg ttttatacag
    31141 aagagacata tgaagaaatt ttcgataatt acgatataga ttattttgta gatgcaagtg
    31201 atacaataat gtacaaagta cacttaatga aagagtgtct tgaccgaggg attaaagtga
    31261 tttccagtat gggtgctgca aataaaacag atccaacacg ttttacaatt tcagatatct
    31321 ctaaaacaca taccgatcca atggccaaaa ttattcgtca aaaattgaaa aaactaggaa
    31381 ttcgtaaagg gattccagtt gtattttcag atgaaagtcc aattgtcata agagaagatg
    31441 taaaagatat agttggagat aaaaatgcta tcaatcgaaa aggacaaatg ccaccttctt
    31501 caaatgcctt tgtgccaagt gtagttggat taatttgtgc aagttatgtg gtgaatgacg
    31561 tattaaaaga tattccagtt cgtcgcatta aagacaaagg gcaataatta aaattgaaag
    31621 tcataatttt caacgtaagg caagtgtaac cataccataa aactaaaact gactagttcg
    31681 caagcattgt agaatatgcc tcgtgtacta gtcagttttg ttttgattat agctaaaata
    31741 tcaaaggtaa tcgatttaat cttattttta tggcctttgt ttaagtaaat tgttatatat
    31801 ttctttgagt tgtcgttcac ttttagatgt cgtttttggt tcataataaa ttttgttttt
    31861 aagtttatca ggtaaatatt gctgtgaaac atagccatta acatattggt gtggatattt
    31921 gtaaccaata gatcggccta aatctttagc accttgataa tgtccatctt ttaaatggtt
    31981 tggaatttgg cccacatgac cgtttctaat atcggacaat gcactatcaa tggcactcat
    32041 tgctgaattt gacttaggtg ataagcatag ttcgattact gcttggctta gtgggattct
    32101 agcttctggt aaacctagac gttctgctga ttcaatagca gcaagtgttc tctgaccagc
    32161 attaggtgag gctaagccta tatcctcata gctaattaca agtaatcgtc gaactattgt
    32221 aggtaaatct ccagcttcaa ttaatcgtgc taaataatgt aaagcggcat tgacgtcgct
    32281 accacggata gatttttgga aagcgctcat aacatcgtaa tgcatgtcac catccttatc
    32341 acttacaaat gcaccttttt gtaagcagtc tttagcatct tgcaatgtaa catgtcgata
    32401 accgtccttg tcattatctg cgcttaatac agctaattcc aatgcattta acgcactacg
    32461 aacatcacct tgactttgtg tagaaaagta ggtcatggca tcttcatcaa ttttgggttg
    32521 atatgttttc aaaccattct cttcatcttc tattgcacga gttaacgctt ggcgcacatc
    32581 ttcgtcattt aaaggatata actcgaaaat ttgcgctctt gaacgaatcg ctggattgat
    32641 agcatgataa ggatttgaag ttgtagcacc gatcaagacg attttgccat tttctaaatg
    32701 aggtaataaa aaatcttgtt tggctttatc tagtcgatgg atttcatcta ataacaagat
    32761 aacttgacca gacattttag cttcttcaac aacaagttgc atatcttttt tagtgttagt
    32821 tacagcattc aattgtctga atttatattg cgtactgccc gaaattgctt tggcaatact
    32881 tgttttgcct ataccaggtg gaccataaaa aatcattgaa gttaattttt ttgtgtcaac
    32941 cattcttctg ataatgcctc ttggtccaac taagtgttgt tgggaaatga tttcatctat
    33001 attttttggg cgcattctcg acgctaatgg ttctgtactc acatttgtca cacctttctt
    33061 atactatctt aactcaaaaa atgctaaaat gggaatatca atattcgaat taagtgaggt
    33121 tatacaatga aaatttctac taaagggaga tatggactta cattgatgat ttctcttgct
    33181 aaaaaagagg ggcaaggatg tatatcatta aagtcaattg ctgaagaaaa taatttgagt
    33241 gatttatatt tagaacagct tgtaggtcct ttaagaaatg cggggttaat tcgaagtgta
    33301 cgcggtgcta aaggtggata ccaattaaga gtgccagcgg aagaaatctc agcaggggat
    33361 attataagac tgttagaagg tccaattaca tttgttgaaa gtattgaatc agaaccacct
    33421 gcgcaaaaac aactatggat tcgcatgaga gatgcagtga gagatgtttt agataataca
    33481 acattgaaat atttagcgga atatgtagat acaagtgaag atttagacgg atacatgttt
    33541 tatatttaac cgtataaata ctatactata acataaaaac ttcatattat aatgtttagc
    33601 gaacctcctt tgtggtatat aaatatatac atctaaggag gtttcactat ggcagacgaa
    33661 agtaagttcg atcaatttaa aggcaatgtt aaagaaacag taggtaacgt tactgataac
    33721 aaagaattag aaaaagaagg tcagcaagat aaagcgactg gtaaagcaaa agaagttgtt
    33781 gaaaatgcta aaaacaaaat aactgatgca attgataaac ttaaaaaata agttcaatta
    33841 aactaatcaa atataagagg agcgattaat atgagtttta tagataaagc taaagacgca
    33901 gtagaaaaat ttaaaaatag cgataatgaa caagttaaaa atgtaaaaga taagataaat
    33961 gaatatacag gatctaataa cgaagagaaa aaagaaaatg aagataaaga gaaataaatt
    34021 tctttaaatt ttggatatga ttttcaatat tttgaaagcg ccccatcaaa taaacactta
    34081 cacagtgtaa aat
//
LOCUS       DAKSQE010000017        33169 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.017, whole genome shotgun sequence.
ACCESSION   DAKSQE010000017 DAKSQE010000000
VERSION     DAKSQE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 33169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33169
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.017"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..146
                     /gene="farR"
                     /locus_tag="O0V33_000674"
     CDS             <1..146
                     /gene="farR"
                     /locus_tag="O0V33_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HCX8975341.1"
                     /translation="DIPDNLIFYIYDSLIEGFIHWIKDEKIDWPGEDIDNIFHKVINI
                     KIK"
     gene            complement(223..390)
                     /locus_tag="O0V33_000675"
     CDS             complement(223..390)
                     /locus_tag="O0V33_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975342.1"
                     /translation="MRYVISIIMGIVLAIWSFKQLSQSHLDSGFMFFFIVYVLYISYF
                     NSDKHDKNKKR"
     gene            complement(502..2046)
                     /gene="pruA"
                     /locus_tag="O0V33_000676"
     CDS             complement(502..2046)
                     /gene="pruA"
                     /locus_tag="O0V33_000676"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HCX8975343.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(2236..2835)
                     /locus_tag="O0V33_000677"
     CDS             complement(2236..2835)
                     /locus_tag="O0V33_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCX8975344.1"
                     /translation="MTEKEKMLAEKWYDANFDQDLINERARAKDICFELNHTKPSDKN
                     KRKELIDELFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFHHRNEGFEKAGPINIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNEVPSEALNDETLN"
     gene            3074..3265
                     /gene="cwrA"
                     /locus_tag="O0V33_000678"
     CDS             3074..3265
                     /gene="cwrA"
                     /locus_tag="O0V33_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HCX8975345.1"
                     /translation="MRILITGTVAILIILGLVKTIQDYEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            3505..5913
                     /locus_tag="O0V33_000679"
     CDS             3505..5913
                     /locus_tag="O0V33_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCX8975346.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVAVETVELDITGMTCAACSSRIEKVLNKMDGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNRDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLITLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLTFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGCPVVT
                     DYHGDDQTLQLLATAEKDSEHPLAEAIVNYAKEKQLTLTETTTFKAVPGHGIEATIDH
                     HHILVGNRKLMADNDISLPKHISDDLTHYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQITKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
     gene            6458..6664
                     /gene="copZ"
                     /locus_tag="O0V33_000680"
     CDS             6458..6664
                     /gene="copZ"
                     /locus_tag="O0V33_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCX8975347.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSAEVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
     gene            complement(6754..7752)
                     /locus_tag="O0V33_000681"
     CDS             complement(6754..7752)
                     /locus_tag="O0V33_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCX8975348.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNLIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKDAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTLNDPLLESLIDREDVILTPHIAFYTEAAVENLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(7775..8929)
                     /locus_tag="O0V33_000682"
     CDS             complement(7775..8929)
                     /locus_tag="O0V33_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCX8975349.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSKVDSKIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKDTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKARRDRFEAMLTKADLPFFHAKGGIYVWLETPPGYDSEQFEQ
                     FLVKEKSILVAPGKPFGENGNRYVRISLALDDQQLDEAAIRLTELAYLYE"
     gene            complement(9356..10864)
                     /locus_tag="O0V33_000683"
     CDS             complement(9356..10864)
                     /locus_tag="O0V33_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCX8975350.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTMCGKNYEDYIELRQLRYIYDVYFDRDDCITVPTDL
                     AELQHMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRSDAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFADD
                     FRGNIEEIFEGRLSHDPSIYVYVPAVADKSLAPQGQTGIYVLMPTPELKTGSGIDWSD
                     EALTDQIKDVIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIEQGV"
     gene            complement(10876..11739)
                     /locus_tag="O0V33_000684"
     CDS             complement(10876..11739)
                     /locus_tag="O0V33_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HCX8975351.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEYHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFENERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFRDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVEKRKKAKL
                     FHEINSKYHRI"
     gene            complement(11776..12903)
                     /gene="crtQ"
                     /locus_tag="O0V33_000685"
     CDS             complement(11776..12903)
                     /gene="crtQ"
                     /locus_tag="O0V33_000685"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HCX8975352.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETACVARSYGATVVDVVDDADGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVEALINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMAAIVLWLFGSIASILGLCLSLKYRQMSVGKMLTVYLSYTTQFIYLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            complement(12909..14402)
                     /locus_tag="O0V33_000686"
     CDS             complement(12909..14402)
                     /locus_tag="O0V33_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCX8975353.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRH
                     ESDDFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGTRVDNIKTYERCVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLLHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKIGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREAKNRK"
     gene            complement(14395..14883)
                     /gene="crtO"
                     /locus_tag="O0V33_000687"
     CDS             complement(14395..14883)
                     /gene="crtO"
                     /locus_tag="O0V33_000687"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HCX8975354.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKHIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHKLNRNIYDQRHLMTINIDEIEKMIIETKRAEL
                     IHWISILPVIIFNKGPRVVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            complement(15064..15831)
                     /locus_tag="O0V33_000688"
     CDS             complement(15064..15831)
                     /locus_tag="O0V33_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCX8975355.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWASAAASSGYTVNNTPKAGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
     gene            complement(16192..18003)
                     /locus_tag="O0V33_000689"
     CDS             complement(16192..18003)
                     /locus_tag="O0V33_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCX8975356.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKSQFARTVLVILLLIPSI
                     IVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
     gene            complement(18689..19390)
                     /gene="isaA"
                     /locus_tag="O0V33_000690"
     CDS             complement(18689..19390)
                     /gene="isaA"
                     /locus_tag="O0V33_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HCX8975357.1"
                     /translation="MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVPASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLGAWGF"
     gene            complement(19998..21053)
                     /locus_tag="O0V33_000691"
     CDS             complement(19998..21053)
                     /locus_tag="O0V33_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCX8975358.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGILILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGITGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            complement(21180..21309)
                     /locus_tag="O0V33_000692"
                     /pseudo
     CDS             complement(21180..21309)
                     /locus_tag="O0V33_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21464..22033
                     /locus_tag="O0V33_000693"
     CDS             21464..22033
                     /locus_tag="O0V33_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCX8975359.1"
                     /translation="MTESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSGSFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
     gene            22231..22731
                     /locus_tag="O0V33_000694"
     CDS             22231..22731
                     /locus_tag="O0V33_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975360.1"
                     /translation="MTSIFVFAYLNNTSGNMLFRQQMICPCLLANVRHSSIVCLNNTC
                     RIVDTRCLNTKIGGIGVSETDEPQGFEHTHNRLNINQNSQSLMTFITSKLKSKLKQHI
                     IIARGNKRIDYRLSYNFYTTYYDNVEIKKIDCEYTYAMAIAILIRHTMSFDNAHFFAQ
                     LRHPVL"
     gene            22951..23181
                     /locus_tag="O0V33_000695"
     CDS             22951..23181
                     /locus_tag="O0V33_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCX8975361.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(23344..23721)
                     /locus_tag="O0V33_000696"
     CDS             complement(23344..23721)
                     /locus_tag="O0V33_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCX8975362.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGTVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(23738..24559)
                     /locus_tag="O0V33_000697"
     CDS             complement(23738..24559)
                     /locus_tag="O0V33_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HCX8975363.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDSVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYVFEHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQDYNK"
     gene            complement(24578..24877)
                     /locus_tag="O0V33_000698"
     CDS             complement(24578..24877)
                     /locus_tag="O0V33_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HCX8975364.1"
                     /translation="MSLNKEQRRITAEELQAHFEESTLSVQMIAGKLNVTTEDVEKAL
                     AMKAPLGIFSHQLQRFIHLVWDVRDVINDNIKENGQTPEPYTYLKGEKEDYWFLR"
     gene            25008..25565
                     /locus_tag="O0V33_000699"
     CDS             25008..25565
                     /locus_tag="O0V33_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCX8975365.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDHKALL
                     QCIEAGNNKLRFYQSAFYQELFYFYYDLFKSDDDIYTKFKTDMLLQALSTRVFDFQIE
                     HRNISIEAYRNYLLNIYLDEVERND"
     gene            25558..26262
                     /locus_tag="O0V33_000700"
     CDS             25558..26262
                     /locus_tag="O0V33_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCX8975366.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSTPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIDA
                     DGPTGTFINGNGEMPW"
     gene            26360..27370
                     /locus_tag="O0V33_000701"
     CDS             26360..27370
                     /locus_tag="O0V33_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCX8975367.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTG
                     FTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIA
                     QYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKT
                     AENLGVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHPVSYYFKNNFYITPSGMLTKP
                     QFDLVKKEAGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSS
                     NN"
     gene            27390..28220
                     /locus_tag="O0V33_000702"
     CDS             27390..28220
                     /locus_tag="O0V33_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCX8975368.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGDIFLPLAEQLKD
                     HFTVVAVDRRDYGESELAEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     SSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGM
                     KTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWSKFEIRQYTHSDITLDDFTKY
                     SDKITLLNGTDSRGSFPQDVNFYINKETGIPIVDIPGGHLGYIQKPEGFADVLLNMWG
                     "
     gene            complement(28367..29257)
                     /locus_tag="O0V33_000703"
     CDS             complement(28367..29257)
                     /locus_tag="O0V33_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCX8975369.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVVDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVSQMTATSDNKHESLHKTINHQFVQ
                     SPAKCTKAQFIKRLACLPTHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(29357..30697)
                     /locus_tag="O0V33_000704"
     CDS             complement(29357..30697)
                     /locus_tag="O0V33_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HCX8975370.1"
                     /translation="MLEVKYDMRTGHYTPIPNEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAVIDQTGFKSWMIWAIEPSMLRIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            complement(30691..31797)
                     /locus_tag="O0V33_000705"
     CDS             complement(30691..31797)
                     /locus_tag="O0V33_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HCX8975371.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISVDDAYYTIATTTETYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNDGQYHISFDSGQSVLTPHEPIVATGFDATKNPI
                     VQQLFATTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQEVIENYQKNQMYLDDYSCCEVSCTC"
     gene            complement(32130..32426)
                     /locus_tag="O0V33_000706"
     CDS             complement(32130..32426)
                     /locus_tag="O0V33_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HCX8975372.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFDNMKQNLDSEIQRGEVTPQ"
     gene            complement(32414..32782)
                     /locus_tag="O0V33_000707"
     CDS             complement(32414..32782)
                     /locus_tag="O0V33_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975373.1"
                     /translation="MSNKLDGINKMITAKHKQMDDLYDEKQEVKALIDESDALNHSIE
                     QLYQHLGERYYSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHDIRKAEEEMIDL
                     EMRRNIEIEMVTKEENKWKQ"
     gene            complement(32797..33075)
                     /locus_tag="O0V33_000708"
     CDS             complement(32797..33075)
                     /locus_tag="O0V33_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HCX8975374.1"
                     /translation="MMSTVQIDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLISKNQSVAEAIRTASDNIQKVGEAFDQTDVMIGNEIGKN"
BASE COUNT        10651 a         6208 c         5129 g        11181 t
ORIGIN      
        1 gcgacatccc agataatctc atattttata tttatgactc gttgattgaa ggttttatac
       61 attggataaa agatgaaaaa attgactggc ctggcgaaga tattgataac attttccata
      121 aggtcattaa tattaagatt aaatagtaaa ttagaggctc ataagcgtta ccaacattca
      181 taataaaaac gatagtgtac acgttaatga attcgtgtac tactatcgtt ttttattttt
      241 atcgtgctta tcgctattaa aataactgat atacaacaca taaactatga agaaaaacat
      301 aaatccgcta tctaaatgac tttgactcag ttgtttaaat gaccaaattg ctaatacaat
      361 tcccattatt attgaaataa cgtatctcac attcttatac ctagaatcct tttctaaaaa
      421 tatggttgct attacttaat ttttaaagtt ataaataaaa agagccaacc gcaatggatg
      481 gcccttgttc attatgaagc attagaacat ttctgaaaca accttttgtt ctaagaagtg
      541 taataagtag tctgggctac ctgttttagc gtccgtacct gacattttga aaccaccaaa
      601 tggatggtat ccaacaactg ctgaagtaca gcctctgtta aggtataaat tacctacatc
      661 aaattcgttt accgctttaa tccaatgctc gcgattattt gtaatcactg caccagttaa
      721 accgtaatct gtatcatttg caacctcaat tgcttcatca aaatcgttaa ctttcacaaa
      781 gccaacaact ggaccaaaaa tttcttcttg catgattcta tctttagatt taagtcctga
      841 aatgattgtt ggttctacaa agtaaccttt tgaatcatca gtgccgccac cttgttctaa
      901 tttaccttct tctttaccaa tttcaatata atttttaatc ttatcaaatt gttttttatt
      961 aataactggg cccatatacg tattgtctac agtattaccc aacgttaatt cttttgttag
     1021 tttaattgat ttttctaata cttcgtcata aacgtcttta tgcacaattg cacgtgaaca
     1081 tgctgaacat ttttgaccag agaaaccaaa cgctgacgtt acaatagctt ctgctgccat
     1141 atctgtatca atattttcat caactacaat ggcatcttta ccgcccattt cagcgataac
     1201 acgtttcaag aagttttgac cttcttgaac aacggcacta cgttcataaa ttctagtacc
     1261 tgtcgcacgt gatcctgtaa atgtaacaaa atgcgtatct ttatgatcaa ctaagtaatc
     1321 accaatttct ttcggatcac caggaacaaa gttaactacg ccttttggta atcctgcttc
     1381 ttctaaaatt tccattaatt tataagcgat ataaggtgta tcctcagcag gtttcaataa
     1441 cactgtatta cctgccacaa ctggtgctaa agttgtacca gccataatcg caaacgggaa
     1501 gttccacggt ggaattgtaa cacctgtacc aattgattta tagaaatatt tattgtgttc
     1561 accttcacga tcaagtactg gcttgccttg agccaagtcc atcattgaac gtgcatagta
     1621 ttcaataaaa tcaatacctt cagctgcatc accaactgct tcatcccatg gcttacctgc
     1681 ttcataaacc ataattgctg caatttccgc ttttcgacga cgaataattg ccgaaacacg
     1741 taacataagc tctgcacgat catttgctga ccatgttttc caagatttat aagcttcgtt
     1801 cgctgcttta aatgcatctt caacatcttg ttttgttgcc tttgatgcat ttgcaatcac
     1861 ttgtgatgtg tctgcaggat tgattgattt aattttgtca tctttgaaaa tcttctctcc
     1921 attaatcact aatggtatgt cttgacctaa ttctttttcc acgtctttca atgctttctt
     1981 aaacatatcc acattttctt ggactgaaaa atcgtaacca ggttcatttt taaattctac
     2041 taccatgtac acttaccccc tataaatttt gaaagtggtt taaccctttg atttaatgat
     2101 ataacatcat ttaaactcat tttactatga gtaaggttgg ttttgcaatc gctttcattt
     2161 ttatgcttta tcccttattc tcaagtattt tgaaattgat tggttacttt ttaaaattta
     2221 gaagtgtcat gacaactaat ttagtgtttc atcgttcaat gcttctgatg gtacctcatt
     2281 atcaatttta cgaacgactt tacaagggtt tccaaccgct aaactgtgtg gcggaatatc
     2341 tttggtgaca acactaccag caccaatcac actgccttct ccaatcgtca ctcccggaag
     2401 cacggctaca tgtccgccaa accaagtatt actgccaata ttaattggtc ctgctttttc
     2461 aaatccttca tttctatgat gaaaattaag tggatgtgtt gctgtgtaga atccacaatt
     2521 aggtcctata aaaacattat cgccaattgt aatctgtcca ccatccataa aataacaatt
     2581 ggtgtttaca tagacatttt ttcctagttt aacgttccaa ccataatctg tatcaaaagg
     2641 aatcgaaata cttacattgt ctgttgttgt ttgaaataat tcatcgatta attcctttct
     2701 tttatttttg tcactcggct ttgtatgatt taattcaaag caaatatctt tcgctcgtgc
     2761 acgttcattg attaagtctt gatcaaagtt tgcatcgtac catttttctg ctaacatttt
     2821 ttctttttca gtcattacac ctttcaactc ctaataactt atttacttgt ttaatagtta
     2881 atcaaataaa cattcgccta tgcaactaat aagctataac attatgaaat cataacctta
     2941 tcactcttat ctataccatt ctcgcatcaa atactgctaa agtagtagat aaattcaata
     3001 ctacagacgc attcattttt taatctatta acgtaaaatg tgagtaagag aaatataaag
     3061 gagtatgata gcgatgagaa tattaattac aggcacagtt gctatcttaa tcattctagg
     3121 tttagttaaa acgatacaag attacgaaat gacaaacgac acaagtcgtc agttgtcaga
     3181 caacaaagat gacgataaag tcatccatct taataatttc aaaaatttac atgcgaaaga
     3241 atttaaccca tctgatttct tttaagtcac ctaagaatta caaatccaga agtcatttaa
     3301 gttttacctt tcattcatac atcctttaat attaattacg acttctttta tatagatgct
     3361 aaatagagag attgttgtgc aatgtttgca cggcaatctc tctttttctt tttaaaattg
     3421 gtaaaagtaa aacgcaacga ttgacttata tacctatagg gggtacatta gacgtgtaac
     3481 aatgaatcac agggaggcaa taatgtggct aatacgaaaa aaacaacatt agatatcact
     3541 ggtatgactt gtgccgcatg ttcaaatcgt atcgaaaaga aactgaataa acttgatgac
     3601 gttaatgccc aagtgaattt aactacagag aaagcaactg ttgagtataa ccctgatcaa
     3661 catgatgtcc aagaatttat taatacgatt caacatttag gttacggtgt cgctgtagaa
     3721 actgtcgaat tagacattac aggcatgact tgtgctgcat gctcaagccg tattgaaaaa
     3781 gtgttaaata aaatggacgg cgttcaaaat gcaacggtca atttaacaac ggagcaagct
     3841 aaagttgact attatcctga agaaacagat gctgataaac ttgtcactcg cattcaaaaa
     3901 ttaggttatg acgcatctat taaagataat aatagagatc aaacgtcacg caaagctgaa
     3961 gcgctacaac ataaattgat taagcttatc atatcagcag tattatcttt accgctatta
     4021 atgttaatgt ttgtacatct tttcaatatg catataccag cactatttac gaatccatgg
     4081 ttccaattta ttttagctac acctgtacaa tttattattg gatggcaatt ttatgtaggt
     4141 gcttataaaa atttaagaaa tggtggcgcc aatatggatg tacttgttgc tgttggtaca
     4201 agtgcagcat atttttacag tatttatgaa atggttcgtt ggctaaatgg ctcaacaaca
     4261 caaccgcatt tatactttga aacaagcgcc gtactaatta ccttaatctt attcggtaag
     4321 tatttagaag ctagagcgaa gtctcaaaca accaatgcgc ttggcgaatt attaagttta
     4381 caagctaaag aagcacgcat tttaaaagac ggtaatgaag tgatgattcc tctaaatgag
     4441 gtacatgttg gagatacact tatcgttaaa ccaggtgaaa agatacctgt tgatggcaaa
     4501 attattaaag gtatgactgc catcgacgaa tctatgttaa caggtgaatc tatccctgtt
     4561 gagaagaatg ttgatgatac tgtaattggt tcaacgatga acaaaaacgg tactattact
     4621 atgacagcaa caaaagttgg cggggacact gcattggcaa atattattaa agttgtcgaa
     4681 gaagctcaaa gttctaaagc gccgattcag cgattggcag atattatttc tggttatttc
     4741 gttcctatcg ttgttggtat cgcactatta acatttatcg tgtggattac tttagttaca
     4801 ccaggtacat ttgaacctgc acttgttgcg agtatttccg ttctcgtcat tgcttgtcca
     4861 tgcgcattag gacttgctac accaacttct attatggtag gtactggtcg cgctgctgaa
     4921 aatggtattt tatttaaagg tggcgagttt gttgaacgca cacatcaaat tgataccatc
     4981 gttttagata agacgggtac cattacaaat ggttgtccag tcgtgacaga ttatcatggt
     5041 gacgatcaaa cgctacaact acttgctact gctgaaaaag attctgaaca cccattggca
     5101 gaagccattg tcaattatgc aaaagaaaag caattaacat taactgagac aacaacattt
     5161 aaagcagtac ctggccatgg tattgaagca acgattgatc atcaccatat attggttggt
     5221 aaccgtaaat taatggctga caatgatatt agcttgccta agcatatttc tgatgattta
     5281 acacattatg aacgagatgg taaaactgct atgctcattg ctgttaatta ttcattaact
     5341 ggtatcatcg cagtggcaga tactgtaaaa gatcatgcca aagatgctat aaaacaattg
     5401 catgatatgg gcattgaagt tgccatgtta actggcgata ataaaaacac tgctcaagcc
     5461 attgcaaagc aagtaggcat agatactgtt attgcagata ttttaccaga agaaaaagct
     5521 gcacaaataa cgaaactaca gcaacaaggt aagaaggttg cgatggttgg tgacggtgta
     5581 aatgatgcac ctgcattagt taaagctgat atcggtatcg ccattggtac aggtacagaa
     5641 gttgctattg aagcggctga tattactatt cttggtggcg acttgatgct tattcctaaa
     5701 gccatttatg caagtaaagc aaccattcgt aatattcgtc aaaatctatt ttgggcattc
     5761 ggctataata ttgccggtat ccctatagct gcattaggct tacttgcgcc atgggttgct
     5821 ggtgctgcaa tggcactaag ttcagtaagt gttgtcacaa acgcacttag attgaaaaag
     5881 atgcgattag aaccacgccg taaagatgcc tagattcctt aataatgaag gattcgttgg
     5941 tgattctgag ataggctagt gattggctct ataatgtcgc ggtttacagt tggatcttcg
     6001 ctccaactgc atatatagtt acacttttcg cttggcgaat tagtgtatct tgggagtggg
     6061 acagaaatga tattttcgta aaatttattt cgtcgtccca ccccaacttg cattgtctgt
     6121 agaaattggg aatccaattt ctctatgttg gggccccgcc ggcaaggttg actagagttg
     6181 aaaaaagctt gttacaagcg tattttcgtt cagtcaacta ctgccaatat aactttgtag
     6241 agcattgaac attgatttat gtctcagact ctacaatatt attacaaata ataagtacac
     6301 tatggtttac agttggatct tcgctccaac tgcataagag ccactaatta ttataaataa
     6361 taagtacact atggtttaca gttggatctt cgctccaact gcataagagc cactaattat
     6421 tataaataat aagtggctct aaaaggaggt gaatgctatg tcacaagaaa ttttaaatgt
     6481 tgaaggtatg agctgtggtc actgcaaaag tgctgttgaa tctgcattaa ataatattga
     6541 cggtgtcact tcagctgaag ttaaccttga aaatggtcaa gtaagtgttc aatatgatga
     6601 cagtaaagtt gctgtatctc aaatgaaaga cgcaattgaa gatcaaggtt acgatgtcgt
     6661 ttaattaggc aatattcaac gtcatcaaca ccaaattaaa aaatcgaact gatgagaatc
     6721 ccaacaatcc aaattatctc atcagttcga tttttaattt actcgtaacc tagtatctcc
     6781 agtctgcaat acatctaatg ttgcatctaa tgcatcaaca attagatttt caactgcagc
     6841 ttcagtataa aacgcaatat gtggtgttaa tatgacatct tccctgtcaa tcaacgattc
     6901 taacaatgga tcgttcagtg ttttgcccct ttgatcactt gggaaaagtt tgcgttcaaa
     6961 ttcatacgta tcaagtgctg cacctttaat cacaccattg tctaatgcgt ctaataaagc
     7021 cttagtatct actaaagaac ctctcgcaca attgacaaat actgcgtcct ttttaaaatg
     7081 tttaaataat tcagcattaa atagataatg attatatttc gttgcaggta catgtaatgt
     7141 cacgatatca gcaccttcaa ccgcttcctc aatcgtatct ttgtaatcga catacgttgc
     7201 aattttagca ttaggaaacg ggtcgtatgc gaccacatca ctttgataac cattggcaaa
     7261 tatatcggct actacacggc caattcgacc tgtaccaata acagctactt ttaaatcttt
     7321 aatggatttc gataaaatag taggttccca tctaaaatca tgctctcgca ctttcgtttg
     7381 aatttgatta aaatgacgaa ccacattaat agcctggttc acagcaaact ccgcaattga
     7441 attcggagag tatgacggca catttgacac aatcaagtta tacttgtttg ctaactccaa
     7501 atcatatgtg tcaaatccag cactacgttg tgcgatttgt ttaataccta gttcatttaa
     7561 tcgtttataa acatgctctg ataatggtat ttgttgtgat agcgataagc catcataacc
     7621 agcgacacct tcaacattgt catcagttaa tgcttcttta gtaatatcta cctcaacatg
     7681 atgtttctct gcccacgcct tgatataagg catatcttca tcacgtacac tcatgatttt
     7741 aatttttgtc attttaacat cacccttaac tttattattc atataaatat gctagttctg
     7801 ttaatcttat tgcagcttcg tctaattgct ggtcatctaa cgccaatgag attctcacat
     7861 aacgattacc attctctcca aatggtttcc ctggagcaac aagtattgac ttctctttca
     7921 ctaaaaattg ctcaaattgc tcgctgtcat aaccaggcgg tgtttccaac catacatata
     7981 tgccaccttt agcatgaaaa aatggcaaat cagcttttgt aagcatggct tcgaatcggt
     8041 cacgacgtgc tttaaataca ttgctttgtt cttctaaaaa atcatcataa tgattcaaag
     8101 catatattgc ggcatcttgt aatgcaccaa acatcccagc atttgtgtgc gtttggtact
     8161 ttttcaaagc ttgaatcata tctttattac caactgcaaa accgactcta aaacctgaca
     8221 tgttataacc tttagacaag gaataaattt caatcgcaac atctttccca ttttccgaag
     8281 caagtatact aggattttta gcgtcgaaac cgaaggcacc ataagcaaaa tcatgcacga
     8341 ttttagtgtc tgtatcttta aatttagcta tcgcttcatc aaaaacttct ttcgtagctg
     8401 tcgatccagt tggattattt ggatacgtta aataaatgag ttttgtttta tctattattt
     8461 ttgaatcaac tttggaccaa tctggcaaat aatgcggcgg ttctaaatta agcggaacag
     8521 gcttgccatc agctaaaagt acacctgcta aataatcagt gtagcctggg tcaggtagta
     8581 atacatagtc tcctggattg ataacacatg ttggtactgc cactaatcca ttttttgtac
     8641 catataaaat acatacttca tcttctttat ctaacgtcac attatattgt ctttgataaa
     8701 aatctacaat agcttgcttg aacgcttctt taccatgaaa agcaccatat ttttgatttt
     8761 caggaatagt tagtgctttt tgaaaatgat caataatacc ttgtggcgtg ggcccatcag
     8821 ggattccaac tgccatatta attaatggca atggtccatg ttcgatttta cgtcccatcg
     8881 ttttcccaaa ataactatca gggatatttg ctaatttgtt agagatcatc aaattcctcc
     8941 tctatcatta aacatagcct gggcgactat cataatccta acaacttgta tcactctcat
     9001 ttagatggtt acaatgacat cgccattcac cgttatcttc aacagatctt atgacacacg
     9061 ttgtattgaa tgaatttatt ttcattttag gtaggtacaa tattattgtc aatattagga
     9121 attttcagat taatatacgc tcaatcgtta ggatttaact gccatgcata tccgcatgcg
     9181 caaccagtta gatatgctta tataaagtat aacgcccatc aaggtacgta ttcaaacgtg
     9241 aaccttaaca ggcgtcattc attgttaaat aaaacttctt aagcacatac ttatttcact
     9301 atgcctttta cgttcccctt atacttttct cacatctttc tcttagacta ctcccttaca
     9361 cgccttgctc aatatcttta atcatttcat ctacagttat tttcgcactc gttaagacaa
     9421 taggaacgcc tgcacctgga tgcgtacttg cacctgcaaa atataaatct ttataatctc
     9481 gcgatacatt ttgtggacga taataattac tttgcgctaa agttggcatt aaaccgaatg
     9541 ccgaaccaaa tttcgcatga tacgtttgct caaaatcatt tggcgtaaag attgtttctg
     9601 aaacaatatg cgattttata tcttcaaata cttcaatagt tgctaattta cgataaataa
     9661 catcttttat ttggtctgtt aatgcttcat ctgaccaatc gattccgcta cctgttttaa
     9721 gttctggtgt tggcattaaa acataaatac ctgtttgtcc ttgtggtgca agagatttgt
     9781 cagcaaccgc aggaacatac acataaatag aaggatcatg cgataatcgc ccttcaaata
     9841 tttcttcaat gtttcctcta aaatcatctg caaaaataac attatgaagt ctcacttgat
     9901 ctgtcacatc aatatctata ccgatataca ttaaaaatgc tgaacaagag taatctaagt
     9961 ctgcaatttt atgtggtgga tactttttaa taggtgcaaa atctggcatt aatgattccg
    10021 caacactagg gaaatcagcc gtacataaaa ttttatcaaa ttttcttatg tcaccattca
    10081 cttttatcgc atcggatcgt ttgaatttag gatcaataat aatttgctca atttcagcat
    10141 taagttcaat attaacgcct aaatctttat ttaattgcgc tagcccttga gccatgccat
    10201 acataccgcc tttaataaaa tgcacaccaa acatcatttc aatcatagga ataattgaat
    10261 atagtgacgg gcctcgtttt ggatcaattc ctatgtataa cgtttgaaac gctaaaagct
    10321 tttgtatctt ttcgttatca atataatgtt caattagctg atctgcatga tttaacgttt
    10381 ttaacttagc accttgcaca agtgacgtca tattataaaa gtcacttggt ttgcgatacg
    10441 ttctttctaa gaaatagcga cgtgcaattt catatttttt ataaacatcc gttaaaaagg
    10501 acataaaacc atgcgttgaa ccaggttcta tactttctag catgtgctgt aattcagcta
    10561 aatctgtagg caccgttata caatcatcgc ggtcaaaata cacatcgtaa atataacgta
    10621 attgtctcaa ttcaatataa tcttcataat ttttaccaca cattgtaaat acatctttat
    10681 aaacatctgg catcatgaca attgtggggc ccatatcaaa tgtaaagcca tctttcttta
    10741 attgattcat acgcccgcct acattattat ttttttcaaa tatcgtcact tcatgacctt
    10801 gagaagcaat acgggctgcc gctgctaatc ctgtgacacc tgcaccaatc actgcaatct
    10861 tcattattca accacctata ttctatgata tttactattt atttcatgaa acaactttgc
    10921 ttttttcctc ttctccacaa aaacacgttc atgtaatgta tagttagcct gtctcacttc
    10981 gtccagtatt tcaatatata tacgtgctgc taattctatg attggttgtg cttcaatact
    11041 aaatactttg atttgatcca taacatctcg aaaatctttt tctgcgatag ctgcataata
    11101 ttcccataag tcaatataat gattattaac accattttgg tacacttcag caatatcaac
    11161 ttcatattgc tttaatcgtt gcttactaaa atatatccgt tcattttcaa aatcttcacc
    11221 gacatctctt aatatattaa tcaattgcaa cgattcacca agccttcttg cgacatcgta
    11281 agtctgatgc gtttcatgat cacttaaaat cggcgtcaat acttcaccta ctgtaccagc
    11341 aacaccataa caatatccga ataattcagc gtccgtttca aacattgtaa aatgttgatc
    11401 tttatataca gtatcaatga gattatagaa agattgaaag gcgatatttt tatgttgtgc
    11461 aacatgctga agggccatca tgattctacg atcactttga aagtgatgat attcatatgg
    11521 gtatttttca atagattgta tatcttcttt tatttgattt aaaaattgaa tatcgccata
    11581 aacatctata ctgtcatcaa ttttacgaca cacagcataa attgcccaaa ccgcttttct
    11641 ttgatcttct ggtaacaagt caaaagcgta agaaaagctt tttgaatgtt tcttcatgat
    11701 tttatgacaa tatttaaaat tcatatccat cattgtcata ctagtcctcc tatattgaaa
    11761 tgccattacc ttgatttatt gttctttaga tatagaatat tgacgacctt tccattcaac
    11821 tacaccataa cgatgcgttt gtttccaaga ttggatgaaa attttagtaa aaaacataaa
    11881 taacaatgga tgacatacca ttaataaatt agaaaattgg ccgacccttc gatgcagata
    11941 aataaattgt gtagtatagc tcaagtaaac tgttaacatt tttcctacag acatttggcg
    12001 atattttaaa ctaagacata gccctaaaat actcgcaata gaaccgaaca accacaacac
    12061 aattgctgcc atgatcttag gctttgtgcc acctgcccca gtcgacaaat gctttgtcca
    12121 tccttcttgt aatgactgaa atccttcttg atacatgcga aatgcaacaa atggaaatcc
    12181 ttcataaact gttacgggca atgattgtga agtatatgca cttcctaaag caaatccttc
    12241 aataatatga cggttcgcac ttttatgacc tccagttgca taataatctt ctttatttgt
    12301 taatgtcacc ggtccaaatg catgctggtt agtccgaccg tcgtctaagg tagaaaatac
    12361 attcatacca acgactgtca ttaaattaaa tattgctgaa aacccttcgt agaaacgctt
    12421 tgttatatga taaggctgta cgcttaacaa tcctttttca ccttgtaatt gatactgatt
    12481 aatcaacgct tcaacagcat ctttccttaa gaaagttaca tcagcatcta caaaagcaat
    12541 acgattcgta catgcatgcg tcacaccttg ataacaagca tgtgatttcc cataccattt
    12601 gccgtcagca tcatcaacaa catctactac agtcgcacca tatgaacgcg ctacacatgc
    12661 tgtttcatct gtcgatccgt cattcataac aatgacatct actggaactt gctgttgtat
    12721 tatcgaatgt agtaaatgac ctattctttt ttcttcgttt ctagccggaa taataattgt
    12781 taatgcttta tgattgaagt tcagcgtttt cgcctttagc tgatgtctac gattaaatat
    12841 caatgcacca cacgccatag acatggtcac tattactgtt aatattcgtg ataaccattt
    12901 catagatatc acttcctatt cttcgcttct cgcgcgttta ttttgtctgc gacttgttgc
    12961 ccacttaatg taaccattgg catgccacca ccaggattta ctgatccccc tacaaagtac
    13021 aagttttcaa aatactgact ttctttagga aatttaaatc ctttgttttt ctttttatct
    13081 gctacaacac catatattgc accacgatta gaacgataat ttttttcaat atcctccggt
    13141 gtccaaacat cttcataaat aatgtgtttt cttaaatcag tgagtcctat tttttctaat
    13201 ttatccaaaa ttttatccct aaacttcgca taatcttcag ttgtaaaagg ctggtcttga
    13261 atatatggaa tatgtggtaa gactttgata ttttcataac ctactggggc ttgtgtgtga
    13321 tcagttttat tcgtatttac taaataaatg gtcggatcat ctggcaatac cttttcatga
    13381 aaaacttgtt gataattgag ataagcattt tccgtaaaaa agaaattatg atgtgctaat
    13441 tgcgggtatt ggcaagcaac acctaaatgc atcacatatc ctgagcttgc tggctcaaat
    13501 tcccgctcta atttgtttaa tcgttgagta tcaaggtgaa gtaaatattt ataagtaggt
    13561 atgacttcca tatttgaaat aatataatct gcttttacaa actcacctgt atctaatctg
    13621 acacccgtca cacatctttc atatgtttta atattgtcca cacgtgtacc tgtatgaatt
    13681 gtgacacctt cttcacgcgc tagcttttcc aaggcattgg ctaaatgatg gattccacct
    13741 tctacatacc aaaggccttg ctcttgttgc atatggaata acatagataa tacagctggc
    13801 gcatcgtacg atgaagaacc tacatatttg ataaaatagc ctaacatttg tcgcaagtat
    13861 ggattcgaga tacgcttgtc tatggcctgt tgcatagtat gtacataatc ataattaata
    13921 agtgcattta atggcccatg aaatttaata atttgagata gtgtatctaa accatagtta
    13981 aaataccctt tttcagtaat acgatcgatt cgtcttgtat aattcaaata attttgcagt
    14041 tcctctatat cctgtttcga caatatcgca ttatgctgac ctgtttcttt aataccttca
    14101 tacaaatcga tagtcgttcc atctggaaaa aagctacgcc attgatgtgg caatcgcttg
    14161 attgtaacgt agtctgacat ttgcttcttg ctatattcga ataatttttc aaaaatataa
    14221 ggcatcgtta aaatagatgg acctaaatca aagccaaagt catctgactc atgacgattc
    14281 actttgcctc ctatatgatt attttgttca tataatgaga ccgaatagcc actttgtgcc
    14341 attcgaattg ctgcagaaat cccacctaag ccaccaccaa taacgatgat atgtttagtc
    14401 atgacgttca cctcttcgtt ttaatatgcg tagtaactgc gttaatctcg gtcgattata
    14461 gcgttgcaca ataatgattg gaacattagc tatcattgca tagaaaatat ttatatactt
    14521 tactacacga gggcctttat tgaatataat gactggaagt atcgatatcc aatgaatcaa
    14581 ctctgccctc tttgtctcta taatcatttt ttcgatttca tcaatattga ttgtcattaa
    14641 atgacgctga tcatatatat ttcgattaag cttatgacca tctaaaatct tatgcttcca
    14701 ttttcttacg taaaaccatt tgttccaaaa ttttccatgc ttctcaaagt taaatgattt
    14761 aaatattata tgcttctgac gaaaatactt gtcaggaatt cttgtaccta aatttgctat
    14821 atttagttga acaattaacc aatacatact acagaaaaat gctgttttga tatatttttt
    14881 catgattttc atctaaattg aatcactctc aatcatactg acatagtata aattatcatt
    14941 ttattactta gttccctaaa gatcagtgaa taaaccgctc tcatttatat ttatatttat
    15001 ataaaacaaa aacgccaata ctctcatgtt agagaatatt gacgtcattt gatttacatt
    15061 tgattaatga atgaaattat atgaacctgc ttggtttgct gaaattgtac gagatgtaac
    15121 cacaccagca ccatgtccat agttcatttc tgaaacacga acagaaccgt tgctgttaac
    15181 gccttcaacg taagcaacat gaccgtaata gccttgtgtt gtttgcatga tagcaccagc
    15241 ttttggtgta ttgttcactg tatagccaga tgatgcagct gcgctagccc aattactagc
    15301 attgccccat gttgaaccaa ttttcccacc aacacgatca aatacataat aagtacattg
    15361 tcctgaagta tataagttac ttcctgatgc ataaccatta gaaattgaac gaccatttga
    15421 agatggcgct gcagttgtag tcacatgaac attattgctt gttgtgctat aacttgcacc
    15481 tgaaccacca gtgtaatagt tatttgttgc tgtttggcta ttgtttgtat agttattgta
    15541 ttgatatgaa tgattatagt agttattgta agatgcattg ttataactat aagtattgta
    15601 gttattgtaa tagtatgtgt tgttttgcgt taattgactt ggattccaat ttcctgtcca
    15661 agtgtaatga taattacctt gtgcatcaat tgtatacgta tagctgtatg attgagcgtc
    15721 attagaatta tatccattgt tattttgttc tgcggcttgt gcatcatgtc ctgcaaatgc
    15781 gatagtggca agtcctgctg tagcgattgt agctgtaacg atttttttca taattaaata
    15841 tcctcccaat aatcaaaaca ctctatacat atagtcatta gcgactcaat tattttaaat
    15901 tttgtatttt ttattttttt agtatgcaaa taattataac ttttatagtt ttgcgacaaa
    15961 acaaactata acagagatta ggtaccttgt ggtctttgtt atcataatgt aatctgttcc
    16021 taacaaagca tcattgaatg taatatacca attagaatgt ttacaacact ttttctgcac
    16081 cgttaacatg ggatttgaaa ataaaaaagc actaaacttt taatgaaata catccttttt
    16141 gataataaat atcagaagta atgtaatatt tcaaaagttt agtgcatcaa attatttctt
    16201 atttgtagca tgtgtttcca tcgtttttac aatcaaatca gtcagcgctt tactacctgc
    16261 atattctaag tgaataccgt catatgcaaa gtactccgga tgacctgcag aagctttata
    16321 ccaatcgact agatgtacat tagagcgctt ttcagctgcc tcataaatta atttatttat
    16381 tctaccttca taatctctag gtactctaat agaaactaaa tatatgtctg cttttccaaa
    16441 actatccaat agttcattta attgatcttt cgtaaatgcc ccatttgtac caagctctac
    16501 tacaactttt tgaccttttt tagcatagtc tttatattgc gatttcacaa ttggtgtagc
    16561 atcaacgagt tgccgtccaa ctttaccatc aatttgtgca tttggtattt tcttagtaaa
    16621 gacattacca atatccacca taaccgagtc accaattaat agtggtgatg actttttaat
    16681 atcctcttta ctattcgccg tctgcttatc ctcttgctta tcttttttaa cgaccttttt
    16741 cttcgttgtt ttaaattccg tcttcttctc tttcttctca gcttcatgtt gtttaccaag
    16801 cgcatcaaat tgcccactga gaacgatgat agatggaatc aataataaga taactaacac
    16861 agttctagca aattgactct tctttttagg taaaaatgca aaagctttaa atcctttttt
    16921 acgtataggt gtttcaataa agcgatacga aatttcagcc attaacgctg ttaacaaaat
    16981 ttctataata taaacgtata ccggtatttg tccttgtacg taataactgt tcacaaaaac
    17041 aatgatagga taatgccata aatataagct atatgatcgt ttacctataa ttagtaaagg
    17101 tttcatactt aaaaatttag caaataaact agaaggatgt accgcaatcg caataatgaa
    17161 taaagttgca aatgatataa tgtaaaatcc tccattatag atccattgat cttggtctcc
    17221 aactataaag aacaaagtca ttagaaccgc aaaaccagat atccctataa tatctaatga
    17281 tacgacaatc tttttagaaa tatctttttt caaagcaaac ggaggccaaa taaatgctaa
    17341 tatacaacca agcaataaag tttgcagtcg tgtatctgtc ccaaaataca cacgtgaatt
    17401 atctccagtg atgaaatgaa tcactatcat aagtcctaaa gaaatcaacg atacaataaa
    17461 tagcgtttga ataatatttc tcggtttaaa tctatgtaat aagaacgtga taaccaatgg
    17521 gaaaagcaag taaaattgtt cttcaatggc taaagaccat aaatgtttga gtggttcaat
    17581 agcaaattgg ttaaaataat ctacattctg tgaaatgtac caccagtttg aaacatagaa
    17641 tatagctgca atagcatctc gtttcatttg tataatcaat tccggtttaa atatcaaggt
    17701 gaacgtaagc acgacacaga ttaaaaataa cactgccgga atgagtcgtt tcaatcgtcg
    17761 cttccaaaac tctagcaaat cgattttttg cgtccgataa tactcactta tcaacaaact
    17821 tgttattaaa taacctgaaa taacgaagaa tgtatctact cctaaaaagc ccccacttaa
    17881 ccattgtgca ttcaagtgat aaataatgat tcctataact gcgaatgccc tcaatccatc
    17941 taatccgggt aagtatcgcg gggaatacat tttttctaaa cgtttaaagt cttttgtatc
    18001 catgttaata aacgccccat ttatttttct ctattttgta gcatatcaca atatttttga
    18061 aaataaaata ttgcactgat tttcattaat tgatttaacc cttaattaag atagttttaa
    18121 attttttatt aagtagaaaa caattattac agttaatttc attactgcaa accacatata
    18181 aatttgtcga ttttactaca taacatagat tatcatagat tcttgaattt ttagcaaaat
    18241 aactgttatt ttcattatat ttttacaaaa aaggttcgtt ttatatttta tgcatcttac
    18301 tgtaacagaa tcattaagat atgctattcg gaatttactt tttcaaaatt tctataatga
    18361 ataaattacc gcgcactgtt attaaaatat atacacggtg atatctttcc acctctcttt
    18421 caaatttaac catatgaaat gacaaatact cactccatat tgaagtattt gtgtaaaaag
    18481 aatcatggta tattgtatat ttcgccttat tctaatttaa tagtaacatt aattcttcta
    18541 tcatgacatt aaaatcgtca ggaataataa aataacatcc taacctatga atcagacttt
    18601 aatttctaat aaaaaaactc tccccaattt ctatgggaag agctatatat ttaatgttta
    18661 aacattactt ttatttatta tgaaggaatt agaatcccca agcacctaaa ccttgtgctt
    18721 tgtatgcttt aacagctgcg ttgatttgtt ggtcaacagt gtttgttggg ccccaacctg
    18781 gcatagtttg gaataaacct gaagcacctg atgggttgta agcatttact tgaccatttg
    18841 attcacgagc gatgattgca gcccatgtag aagctggaac accagtacgt tgagccatga
    18901 tttgagctgc tgatgaacca gtagcacctg cagtattacc attgcttaat ctcactgaac
    18961 ttgaagtagt tgaagtgctg tagttatggt aagttggagc tgaaacagct tcaacgtttg
    19021 agttacttga ttgtgcattg tagcttactg attgtacatt tgaaccttgg ttgtatgaag
    19081 tagtgtagtc tgcacctgca acgtttgaga aaccagcagt ttgaccatta gctgcttcat
    19141 agctccatga ccatgtagta ccatttgaag taaagttata ttggaaacca tcttttacaa
    19201 agtggatgtc atatgcacca tctttgattg gagctgcatt taattgatct tgatgattat
    19261 gcgctaagtc aactaagtgt gcttgatcaa cgtttacttc agcagcgtgt gcttgatgtc
    19321 ctgtacctgc tgcgtaacct gttacaccta atgccactgc taatgatgat gccataattg
    19381 tctttttcat agtaaaaaat cctccagtaa taattgtaag tttatgtttt tagtaattat
    19441 attttgaatt tgaatgtcgt agtgcaagtt taaattgtct tttatttctt tcaacggtac
    19501 tcactatatc ataaaaaagt agcaagtaaa ttacactttc tttacaaaac attacaatat
    19561 caggtgttat ttgtaatgtt gaaatatggt tgttttatac tgtaatgtga aatatgtttc
    19621 ttttagaatc taatcaatcc ttgaaataag cataaatgca taagtttttt actatattta
    19681 gctcaactat tacagctttc gtaatattac agattgtatt tttgttacac gcctgtaaca
    19741 ttaccgtcat caaatataac ttaaattgat taattaaaca caattaaaaa gcaatcaata
    19801 catagattcc ctcacatgta ttgattgcta ttattgttat atattcaaag ttttaaaaca
    19861 catatctttt gtgtattgac ttatctttta taagcacaaa taaactgcag ctcaattata
    19921 ttgctaaact ttattctcgc aattcacaat aacattaaat aatttttggt ctaatatttt
    19981 caaaaaacat attgtcctta tcccatgaat ttaaaaatat cattagtata taaacgaaac
    20041 actttacgat aaatgatatc tgcaagccaa gctgttacaa atggtacaac aaagaacgct
    20101 actacaatta gtaagacact caaccaagca gaatcaacct ccataaattt aaatgcatta
    20161 atcggtccta ccattcctat aaaaccaaat ccagctgact ctttcgttcc atgaatacct
    20221 actaatgctg ataccaatcc tgatacaatg gctgtcgtca atattggtaa cataagaatt
    20281 ggatatttca ccatattagg tatcatcatt ttaacgcctc caaagaagac ggataacggc
    20341 acccctaaac gatttacttt acttgtacca attatcaata ctgcttcagt cgcggaaata
    20401 ccaattgacg ctgatccagc tgctaaacct gtaataccta tcgcaaaggc aatggccaca
    20461 gttgatagtg gcgaaataat aataagacta aataccattg aaatcaaaat actcatgaca
    20521 attggttgta attctgtaaa accattaacc atattaccga tggctgttgt aatcattttc
    20581 gtatacggca atattaaaat accaattgca cctgaaatac cgccaacaac tgttgggaat
    20641 acaatcaaag ccatactacc tacacgatgt tgaataagta aaatgaacaa cactgcaatc
    20701 gctgctgtaa tcattgtatt aattaaatca ccaatacccg taatcatcca agcaccattt
    20761 ttaaactgcg ctgcaccgct tcctacatat gctgcacttg ccacaacagc aattgctaat
    20821 ggcgataggt caaatttcat ggcaaccaat gcaccaatca aagcaggtac tgtaaattga
    20881 attgcaacga caacgcctaa taacgtttta aaaatcggat gataatccat aaagtattta
    20941 aaaatttctc caagtatcgc attaggaact aaacccgcaa caatacctat ggcgacacct
    21001 gataaaactc taaatataaa atctttgggt gtaattgttt taattgatgt cataatatca
    21061 tccttccatt tatgtatata catctttatg caaataataa agagccttaa gttataagct
    21121 gccactagct taaattctaa gatgtgcatg ccgatgttgt tatatttagg ctagcagtat
    21181 catctataac tcaagactat gaaaaatagt atatcacaaa attctgaatt tttagataaa
    21241 gaaattggca atttttcaat catattgtta caatacactt tttattttat cttcattttt
    21301 aaaatccatt aatataatag aagaaagaca ttcaaatgct taccaaaaag atacattatt
    21361 tgttaggagc gtatcagcac ttacatatca tcaacacaat tgacaacata atagaagata
    21421 ctgataataa gtgttaaaac aacagatgtt aggtagtgaa caaatgacgg aaagtaaatc
    21481 catagatcca agaatcgtta gaaccaaaca attgcttgtc gatgcttttc ttaaaatttc
    21541 tagagaaaag aaattgtctc aaattacagt taaagatatc actgatattg ctacgttaaa
    21601 tcgtgcaaca ttttacgctc atttcactga taaagaagac ctcctagact acacattgtc
    21661 tgtaaccatt ttaaaagact tgaatgataa cttgagcatt tctaatgtca ttaatgaaaa
    21721 ggtactgcgt aatattttca tttcaattgc gagttatatc aaagatgctg caaagtcttg
    21781 cgaattaaat agtgaagcat tttgcaacaa agcacatcaa cgtattaata atgaattaga
    21841 agatattttt gcgattatgt tagaaaacag ctacccagag catcaacgag atatcattgt
    21901 aaatagtggg agttttttag cagctggtat atcaggctta gcattacatt ggtttaacac
    21961 gagtcaagaa acagccgatg tgtttatcga tcgcaacctt ccatttttaa ttcatcatat
    22021 agcacatttt taataaaact tggtatttag tcatgcatct tgaaatcact atgtgactta
    22081 ggttcatact tgtacacaca ataaaattta acgtattacg attgattagc cgtgtctagg
    22141 acataaatca acgtcctata ctctacaaag ccatattggc agtcgttaac tgaatgaaaa
    22201 taagcttgtc attaaaacat atagatttta gtgacaagca tttttgtttt tgcgtactta
    22261 aacaacactt caggcaatat gttgtttagg caacaaatga tatgtccgtg tttattggca
    22321 aacgtacgac atagtagtat agtatgtcta aacaacacat gtcgcatagt tgatacgcgt
    22381 tgtttaaata ctaagatagg agggattggc gtgagcgaga cagatgaacc tcaagggttt
    22441 gaacacacgc ataacagatt aaatattaat cagaatagtc agagtctaat gacattcatt
    22501 acaagtaaat tgaagtcgaa gttgaagcag cacataataa ttgctcgtgg taataagcga
    22561 atcgactatc gactgtcgta taacttttac actacgtatt atgataatgt agaaatcaag
    22621 aaaatcgact gtgaatatac ctatgcgatg gccattgcaa ttttaataag acacacgatg
    22681 tcattcgaca atgctcattt ctttgctcag ttacgtcatc ctgtcttata aaacaacatt
    22741 gcagacatgt atatcaaacg gcacttcaat aacatcactt tgccgtcgta ctactattaa
    22801 aatcgtgtct caaacccctt attttaattc caaaaatctg ctggtcaaaa gacccagaaa
    22861 ctaaaaacat tactgaatgt gttgataaat tacctgatag aaataatctc aaaatatatc
    22921 aacacttgat tctaaggagg atatgacaat atgaaaattt tagatagaat taatgaactt
    22981 gcaaataaag aaaaagtaca accacttact gtagctgaaa aacaagaaca acatgcattg
    23041 cgccaagact acttaagcat gatccgggga caagtattaa caacattttc cacaataaaa
    23101 gtggttgatc caatcggtca ggatgtcaca ccagataaag tttatgatct tcgccaacaa
    23161 tacggttata ttcaaaatta atatttgctc acgaggtatt gcacttaagg tgccaactga
    23221 cctcataaac aaagcccata ctgattgaag acactaatgt gtcgcaccat gtctcttacg
    23281 acatgcgcac attacgcttc atctctgtat gggcttttta tttattcttt tgagaatttc
    23341 attttagcag actaaaaaat taaaatggtg tccatcaaga tctttgaaca ttgcaccata
    23401 aaacccttgg cttactgttg gttcttgaac gactgtgcca cccgcttctt tcactttatt
    23461 caccaactgg tcaacttctt catttgtatt cacacctaaa gaaatcatca cttcatttgg
    23521 ttcagtgtta aggctttgct gacttacatt ttgaaaatgc ttgttttcta ttaaaattac
    23581 ggttgtttga cctatttgaa tgcctaccat tttatctaac atttgtgggt ttctatttat
    23641 tttaaatcct aacgctttat aaaactgtgc gctcttttct aaatcttgca catgcaaatt
    23701 aaaccacatt gattgaatca tgattgcacc ccattcatta cttattatag tcttggactt
    23761 taagccaatc acttaatgat aatcttgttg gatttatttc agccattaat tcaaagtcta
    23821 cttcataacc tttttcttcc aaccattgct tttctgcaac accactaaca aattctcctt
    23881 ctataacagt cgatttacct gtcacttcac taaaaattgt tgctgcttca cttaatgtaa
    23941 cttcatcgga accaatctct attgattgat gcgtaaagct ttgtggatgt tcaaaaacat
    24001 acgatgcaat tttagctata tcaatagaag aaatcattgt gaattttata ttcggattaa
    24061 taaattctgg taatgtaata cgttcatctt cgactttagc aatacgtaaa aaattatcca
    24121 taaagaatga tggtttgata actgttgcat ttatattaga ttccattaat ctattttcta
    24181 tttttgctag tacttcaaag tgtgggcctg ttcgatttcg attaacccct cccgcagtgc
    24241 tatacacaat atgttgaata ttttcttgct cagctatttc aattatcttc ataccttgtc
    24301 ttaattcttc gctaacatca tctttaacga ttggctgaat actgtataaa ccatacttcc
    24361 ctttcatcgc tgattgcaaa ctaacactat cactcagatc accttcaacg attgataaat
    24421 gcggatgtcc tatgtctgaa agtttacgat tattcttatt tctagttaat gcacttacat
    24481 accatccatc ctctaacaac tgttttacaa ctgcattacc ttgcttccct gttgcgccta
    24541 ttactaaaat atctttcatt ttagatccac tcccctttta tcttaaaaac caatagtcct
    24601 ctttttcacc ttttaaatac gtatatggtt ctggtgtttg tccattttcc ttaatattgt
    24661 catttattac atctctgaca tcccatacta aatgaataaa tcgttgtaat tgatgactaa
    24721 aaatgcctag tggcgctttc atagctaatg ctttttccac atcttctgta gtaacattca
    24781 gttttcctgc aatcatttga accgataagg tagattcttc aaaatgtgct tgcaattctt
    24841 cagctgtaat acgtctttgc tctttattta aagacatacg caacactctt ttcttttatg
    24901 atatttaata gttattgcaa attcattgct aactttctta acacgtttta taataaacgg
    24961 agagttatcc gtttgtcaat tttagagaac tataagtagg tggaatcatg cgaaaagatg
    25021 caaaagagaa taggcaacga attgaagaaa tagcgcacaa actttttgat gaagaaggcg
    25081 ttgaaaacat tagtatgaat cgtattgcta aagaactagg tatcggtatg gggacattgt
    25141 atcgacattt taaagataaa agcgatttat gctactacgt catacaaaga gaccttgata
    25201 tttttatcac tcattttaag caaattaaag atgactatca ttctaattat gaggttatgc
    25261 aagtgtcgtt agattatctt ttacaattta aaatcgacca taaggcacta ctgcaatgta
    25321 ttgaagcagg caacaataaa ttacgttttt accaaagcgc attttatcaa gaattatttt
    25381 atttttatta tgacttgttt aaaagcgatg acgacattta cacgaagttc aaaacagata
    25441 tgctgttgca agcattatca acacgtgtat ttgatttcca aatagaacat cgcaatattt
    25501 caattgaagc gtatagaaat tatttattaa acatttattt agatgaggtg gaacgtaatg
    25561 actaaaattg tgttaatcac aggtggtaat aaaggattag gctatgcaag tgcggaggca
    25621 cttaaagcat tgggctacaa agtgtatata ggttctcgta atgacgtaag aggacaacaa
    25681 gcatctcaaa aattaggtgt tcattatgta cagttagatg ttacaagtga ctactctgtc
    25741 aaaaatgctt ataacatgat tgctgaaaaa gaaggtcgtc ttgatatcct cattaacaat
    25801 gcaggtatat ctggtcaatt ttcaacacct tctaaattga cacctcgtga cgttgaagaa
    25861 gtatatcaaa cgaatgtatt tggtatcgtt cgaatgatga acacattcgt ccctctctta
    25921 gaaaaatctg aacaacctgt tgtcgtcaac gtatcaagtg gtttaggttc atttggaatg
    25981 gttacaaacc ccgaaacagc tgaatctaaa gtgaattcat tagcttattg ttcgtctaaa
    26041 tcggcagtaa caatgctaac attacaatac gctaaaggat taccaaatat gcaaatcaat
    26101 gctgctgatc caggtgctac aaacactgat ttagttggtg attttagtaa taattcaaaa
    26161 catgtttctg aagggatcaa gccaatcatt caattagcaa ccattgatgc agatggcccg
    26221 acaggtacat ttattaatgg gaatggtgaa atgccttggt aatccaaatt tatgttacaa
    26281 atattgaatt tagaggtaaa taaaaaatgc ttctgcaata gatgcaatat acatgcaaaa
    26341 caatatggag gcgaatcaaa tgaaaagtat tacgtttgaa gaacattatg tcattgaaga
    26401 tattcaaaaa gaaacgatga atgcgatatc ggcagatcct aaaggtgtgc cgatgaaagt
    26461 aatgttagaa ggccttgaaa aaaagacagg ttttacaaat gccgacgaat tatcacatca
    26521 tgatgaacgt atccaattta tgaataatca agacgttcaa attcaagtct tatcttatgg
    26581 aaatgggtct ccttcaaatt tagttggtca gaaagccatt gaattatgtc aaaaagcaaa
    26641 tgatcaattg gcaaactata ttgcacaata tcccaatcgc tttgtaggct ttgcaacttt
    26701 acctatcaat gagcctgaag ccgcagcgcg agaattcgaa cgttgcatca atgatttagg
    26761 attcaaaggt gcgctcatta tgggacgtgc acaagatggt tttcttgatc aagacaaata
    26821 tgacattatt ttcaaaacag ctgaaaattt aggcgtaccg atttatctac atcccgcgcc
    26881 agttaacagt gacatttatc aatcatacta taaaggaaat tatcctgaag taaccgcggc
    26941 aacatttgct tgttttggtt atggttggca cattgatgtt ggtattcatg caatacatct
    27001 agtattatcc ggcatttttg atcgttatcc aaagttaaat atgattattg gacattgggg
    27061 tgagtttatc ccattcttct tagaacgtat ggatgaagct ttattcgctg aacatttgaa
    27121 ccaccctgta agctattact ttaaaaataa tttttatatc acaccgagtg gtatgttaac
    27181 gaagccacag tttgatttag tcaagaaaga agcaggtatt gatagaattc tttatgctgc
    27241 tgattatcca tatattgagc ctgaaaaatt aggtgtattt ttagatgaac tgggtttaac
    27301 agatgaagaa aaagagaaaa taagttatac aaatggtgct aaattattag gtttatcatc
    27361 taacaattaa caaatggagg tcattgaata tggaaacttt agaattacaa ggcgctaaat
    27421 tacgctacca ccaagtcgga caaggacccg tgctcatctt tattcctggt gcaaacggta
    27481 ccggagacat ttttctacct cttgcagaac agttaaaaga ccattttact gttgtagccg
    27541 ttgatcgtcg tgattatgga gaaagcgagt tagctgaacc actccctgat tccgcttcta
    27601 accctgacag tgattatcgt gtcaaacgcg acgcacagga cattgccgaa cttgcaaagt
    27661 cattaagtga tgaacctgtc tatatattag gttcaagttc aggttcaatc gttgcgatgc
    27721 atgtgttaaa agattaccct gaagtcgtta aaaagattgc atttcatgaa ccaccaatta
    27781 atacattttt accagacagt acttattgga aagataaaaa tgatgacatt gtgcatcaaa
    27841 ttttaacaga aggcttagaa aaaggtatga aaacatttgg tgaaacatta aatatcgcac
    27901 caattgatgc aaaaatgatg tctcaacctg ctgatactga agaaggacgt atagaacagt
    27961 acaaacgtac gatgttctgg tcaaaatttg aaattagaca atatacgcat tcagatatta
    28021 ctttggatga tttcacaaaa tacagcgata aaatcacctt actaaatggg acagattcta
    28081 gaggctcctt cccacaagat gttaactttt atataaataa agaaactggc atacctattg
    28141 tagatattcc aggcggtcac ttaggttata ttcaaaaacc tgaaggcttt gctgatgtat
    28201 tattaaatat gtggggttaa ttgatcgttc tctaataaaa cagcttggac agttttcaaa
    28261 ttatatttga tggctgacca agcttatttt tatttacgct taaacgttca aatcaactcg
    28321 ttattataca tgaaggcatg ttaaccattc tcttccactg aaaacactac tgttccaaac
    28381 attgatagtc ttcttttgaa ataccctttc cgataagcac caaatattct ggcacttttt
    28441 ttgaaaatgc tacaggtgtt aattcatatt gtccttgtgt aaattgaatg agatacgtat
    28501 gtgcggtgtc ttcaaatgtc ataaaccctt tcaacctata aatatgagtc ggaaggcatg
    28561 ctaaacgttt tataaactgt gctttagtac atttggctgg tgattgcaca aattgatgat
    28621 ttattgtttt gtgtaatgat tcatgtttat tgtcagaagt tgctgtcatt tggctaactg
    28681 atattggcaa agtgacgctg ccatgcatac caacttgtat atcggcctct gggttaataa
    28741 cttctaaatc tttcaatagt ttgctcgtcg tttcaacatc tgctgaatct attttattaa
    28801 caaataagac agaacaatat gctaattgct cataaaataa gccttggatg tcttttggaa
    28861 atgattttat gtgtttatac atgcttgcat ctacaacacc aatcatatgt gtaattgttg
    28921 taaacggagc taaaatgggc gttaaacaag catctaagac agagactggt tctgcaatcc
    28981 cactacattc aataaataca atgtctggtt gctcttttaa atataattga tgtaactgtt
    29041 ctgatacatc tgctttcatt gcacaacaga tacacccctc tgtcagttca cttaaaggta
    29101 tgtcttcaga tactaaggca ccatcaacac tcattttgcc aaattcattc atgataactg
    29161 ccggtttcag cgactccttt aatgattcag cgagcaaatg atttaagaca gtcgttttac
    29221 cgccacctaa aaacccacct ataataacta ttttcatctg cgactctcct cttatacttc
    29281 atatgaatag catattccaa gatagctttc ctattgactg acatgactct atattcagtt
    29341 atagttcatt tcttttttaa ataatcagca ttactatttt aacaatgatg cctacaccaa
    29401 taactaaaga caatgatgtc accatttgct ttccaattaa ttttagtgct gactgaccac
    29461 ctaaatgttt caaaagcatc gtcattgtga ccgagcacgc tgtaaatgtt gaactaaaaa
    29521 acacaagtag tagtaactgt gttgctgtca ttccttgtaa taaggcgcct tgatgcaaat
    29581 taaacaagag catgccgtct tttcgaatca ttgaaaataa aatccctggt gacaattctg
    29641 acgagatgcc taataacgat aatataggtg taaatatttg tgataaaaca ttcaaaattg
    29701 gcgttagtga taaaatacta acaataagac agattgtgat aaaaataggt agcgcttgaa
    29761 cgataaacat ttgtatattt tgccacattt gtagcaacat ttgacgtata tttggcatat
    29821 gtaattgcct atcataaggt agcggaacgc taagttgttg atcattcttt ttcaaccata
    29881 tcctattatg taagatgcca cctaaaagta ctaatattaa gtacggcata aatagccatg
    29941 actttccagc tacactaaaa atagataatg tcgcacctat ttgataacta caagaactac
    30001 caaagcttat taaactcata cactgtgtct tcgtgcaggt atggcattgg tgtgctgctt
    30061 gtgaaatagc tgctgcatta catccaaatc cttctaatag tggcacgata tcattacctt
    30121 gtaatcctat ccgtaacatt gaaggttcaa ttgcccatat catccatgat ttaaatcctg
    30181 tttgatcaat tacagcagtt gataagctaa tcaatattac gaccggcaat gcccatacga
    30241 gcgaatatgt tccaagtgat agcacaccat aatcaccaaa taatatatgc tgtaatatat
    30301 cgtgattgag caatgttgtt tgagttaaat attctatcca tgctgataca tactgatttt
    30361 gaaaccaatc tgaaaattga taagcgatac cgattggcaa tgcaaacatt aaaaatatca
    30421 acaaccaggc taaaaatgtc gcacttattt taggtcttaa atgtcgtcgt tcatgttgaa
    30481 atagtgtcaa ttgttctatc acatgttcgc tcgctatagg cgaagaaact acactttctg
    30541 ccaatgaaat atctaatttc tgttttatga ttaataatct taatttcgct ttttctgttg
    30601 cggtaagttt atccgcatgg ctaataacca aataatgagg ttcattaggt atgggcgtat
    30661 aatgccccgt tctcatatca tatttcactt ctaacatgtg catgacactt cacaacatga
    30721 ataatcatct aaatacattt gatttttttg gtaattttca atgacttctt gtttagctgg
    30781 taagccttcc cgctgtgtta aaagatgtgc aagtactgca aatcgcgctc tgaatttata
    30841 gatatagcat aatttggcat tatcattttc aactgttgca ccaatcataa aaatattcgg
    30901 ataacgtgtc gattcatcat gtgttgttaa tttaatatct tgatttgttg tcgcaaataa
    30961 ttgttgaacg attggatttt ttgttgcatc aaagccagtt gctactattg gttcatgagg
    31021 cgtaagcaca ctttgtccgc tatcaaaact gatatgatac tgtccatcgt taaaatcaat
    31081 atctttaact gtataatgca cattcatttc aatgcgagct ccttgcttaa tgacattacc
    31141 tagtcgctga cgtgtataag gtgacaatct aacactagga tcagcatccg gatcatttaa
    31201 accggttgtg ctagtataaa gtgcgatgtc agagccattt tttgcaagtt gatatgcagc
    31261 atcaaagcca ctttcattac ctccgataac cacatattgc cccttattaa agttatcaaa
    31321 gtcttcaatt tcactataat gaataccata tttaaatggc tttttaggga aattataatc
    31381 acctgttgcg acaaagatat aatccgcgtg atatgtctct gttgtcgttg caatcgtata
    31441 atatgcatca tctacagata tatttgtgac aactgtattt tcaaagatat tcagctcgta
    31501 atggttggca accacttgta aatattcagc atatgtttct ccggaaatat gttcttcatt
    31561 aaaagtaaat gctggtgaag tatccatgga aattgcattc atatcaggca tgccaaatcc
    31621 attagacgta aatgatggcg taatcgtacg ggtcgatttc ggccaatgtt taaatgaatg
    31681 tcctactgtt cctttttcta aaataatgac atctgttata ccgaaatctt ttaaggtaat
    31741 ggccatacct atacctgcag caccggcacc gataatagcc actttatgat gttgcatatt
    31801 aacactccct caatgatcta tttcaagtta ttgtttttga ttataaatcg taataattac
    31861 gatttataaa gtatagacct atccttgaga aatttcaata ttaaaagaaa aacttccgct
    31921 attgtagaga attcatctac atttcggaag ttggtatgtg tcttttttat taaatttaag
    31981 gatgcaacgg ttagttcaaa tgattatgaa tgacatttca tggtatcatc cagcctcagt
    32041 acttggagat aacattttgg cggtgagaca gaaatgatat ttttacaaaa tttatttcgg
    32101 cgtccctcca ataaatataa agtatttctt tattgtggcg taacttctcc acgttgtatt
    32161 tcactatcca aattttgttt catattatca aacaactctt gaaatctaac ctcatcttga
    32221 tcagaataac ctttaaaaat aactgaatct atattttctt cgttaaaata ataaatttca
    32281 tcttccacaa caccaatcgg atatttacat gcagaatagt caaacatata cttttgattt
    32341 tcaatttcta caattggtcc tctattaata atcattaact tttgcgaacc ttcttttaaa
    32401 taaataatgc ttcctattgt ttccatttat tttcctcctt tgtcaccatc tcgatttcga
    32461 tatttcttcg catttccaag tcaatcatct cttcttccgc tttacgaatg tcatgttgaa
    32521 tttgctgttg ctgctcgtat aacgcttccg ttgaacgtcg tttcgcaaaa tgaaattcat
    32581 cgcggaactg ttccatacga ctagccatat tgctactata ataacgctca cctaaatgtt
    32641 gatataattg ttctatcgaa tgattaagcg catcgctttc atctatcaat gctttaacct
    32701 cttgcttttc atcatataag tcatccattt gcttatgttt cgctgtgatc atcttattga
    32761 ttccatcaag tttattcgac atcatttcac cacgttttaa tttttaccaa tttcattacc
    32821 aatcattacg tcagtttggt caaaagcctc accaactttt tgtatattat cgctcgcagt
    32881 tcgtattgct tcagcaacag attgattctt actaatcaaa tcatctatca cactatgagc
    32941 attattattt ccacttactg ttgtactttc atctttatcc ggtttcgaca cattattaca
    33001 tgtttcaaca gcgcttttaa tagatgatga tgcactatta gtcttaaaaa tatcaatttg
    33061 aactgtactc atcatttaca cctcttccca tttaaattat tttaaaatta ttagaaactg
    33121 tatcaatata tgatattcaa acattcaatt atacttaaat attgttttg
//
LOCUS       DAKSQE010000018        32599 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.018, whole genome shotgun sequence.
ACCESSION   DAKSQE010000018 DAKSQE010000000
VERSION     DAKSQE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 32599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32599
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.018"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            158..955
                     /locus_tag="O0V33_000709"
     CDS             158..955
                     /locus_tag="O0V33_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCX8975375.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDVLGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNDLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKDYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
     gene            1204..1695
                     /gene="lspA"
                     /locus_tag="O0V33_000710"
     CDS             1204..1695
                     /gene="lspA"
                     /locus_tag="O0V33_000710"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCX8975376.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDAQYNLFMQVAISLLFAG
                     ALGNFIDRVLTGEVVDFIDTNIFGYDFPIFNIADSSLTIGVILIIIALLKDTSNKKEK
                     EVK"
     gene            1695..2612
                     /locus_tag="O0V33_000711"
     CDS             1695..2612
                     /locus_tag="O0V33_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCX8975377.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNSDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKPKGMVVH
                     PSPGHYTNTLVNGLMYQIKDLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTGEYIERHAELAQDFEDLLDTIRKRDA"
     gene            3014..3541
                     /gene="pyrR"
                     /locus_tag="O0V33_000712"
     CDS             3014..3541
                     /gene="pyrR"
                     /locus_tag="O0V33_000712"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCX8975378.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            3759..5066
                     /locus_tag="O0V33_000713"
     CDS             3759..5066
                     /locus_tag="O0V33_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCX8975379.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIFVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            5094..5975
                     /locus_tag="O0V33_000714"
     CDS             5094..5975
                     /locus_tag="O0V33_000714"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCX8975380.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            5993..7267
                     /locus_tag="O0V33_000715"
     CDS             5993..7267
                     /locus_tag="O0V33_000715"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCX8975381.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGIDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            7269..8369
                     /gene="carA"
                     /locus_tag="O0V33_000716"
     CDS             7269..8369
                     /gene="carA"
                     /locus_tag="O0V33_000716"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCX8975382.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAILMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            8362..11535
                     /gene="carB"
                     /locus_tag="O0V33_000717"
     CDS             8362..11535
                     /gene="carB"
                     /locus_tag="O0V33_000717"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCX8975383.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHNFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQCLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            11645..12337
                     /gene="pyrF"
                     /locus_tag="O0V33_000718"
     CDS             11645..12337
                     /gene="pyrF"
                     /locus_tag="O0V33_000718"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCX8975384.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQHTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
                     RMLTEKLGTSFLKVTPGIRPKGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
     gene            12337..12948
                     /locus_tag="O0V33_000719"
     CDS             12337..12948
                     /locus_tag="O0V33_000719"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCX8975385.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKQAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            12978..13190
                     /locus_tag="O0V33_000720"
     CDS             12978..13190
                     /locus_tag="O0V33_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975386.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTINHNDDLFKHVKDILRKQGQI"
     gene            13627..14028
                     /locus_tag="O0V33_000721"
     CDS             13627..14028
                     /locus_tag="O0V33_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCX8975387.1"
                     /translation="MDIPKITTFLMFNNQAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DPGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(14291..15988)
                     /locus_tag="O0V33_000722"
     CDS             complement(14291..15988)
                     /locus_tag="O0V33_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HCX8975388.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEID
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKMNPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVNRRQFMTS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLHKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYEHIQKMKQS"
     gene            16263..16886
                     /gene="gmk"
                     /locus_tag="O0V33_000723"
     CDS             16263..16886
                     /gene="gmk"
                     /locus_tag="O0V33_000723"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCX8975389.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLDHLRERLVGRGTESDEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            16886..17104
                     /locus_tag="O0V33_000724"
     CDS             16886..17104
                     /locus_tag="O0V33_000724"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCX8975390.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            17320..18519
                     /gene="coaBC"
                     /locus_tag="O0V33_000725"
     CDS             17320..18519
                     /gene="coaBC"
                     /locus_tag="O0V33_000725"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCX8975391.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            18519..20927
                     /gene="priA"
                     /locus_tag="O0V33_000726"
     CDS             18519..20927
                     /gene="priA"
                     /locus_tag="O0V33_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCX8975392.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIEVPSTLLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEERTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMEASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
     gene            21430..22383
                     /locus_tag="O0V33_000727"
     CDS             21430..22383
                     /locus_tag="O0V33_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975393.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKES
                     EEEKDPDLEKFEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKV
                     ELADYRFTKDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNS
                     NTPVFYAEIMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKP
                     VPYSVAQIKSFGVPLTSVSFMTHGSKDTKDEVMPALAYFTFSPKNYEDKSNPDPKVLN
                     LVGMDFLNASSDFGNAHFVVLSKYIKEYESNYETASDDSLK"
     gene            complement(22546..22749)
                     /locus_tag="O0V33_000728"
     CDS             complement(22546..22749)
                     /locus_tag="O0V33_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCX8975394.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
     gene            23048..23536
                     /locus_tag="O0V33_000729"
     CDS             23048..23536
                     /locus_tag="O0V33_000729"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCX8975395.1"
                     /translation="MAIKKLVPASHPILTKKAQAVIKFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            23529..24464
                     /locus_tag="O0V33_000730"
     CDS             23529..24464
                     /locus_tag="O0V33_000730"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCX8975396.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPKLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            24461..25768
                     /gene="rsmB"
                     /locus_tag="O0V33_000731"
     CDS             24461..25768
                     /gene="rsmB"
                     /locus_tag="O0V33_000731"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCX8975397.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNVMDKALF
                     TEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEETETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVDLQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            25771..26865
                     /gene="rlmN"
                     /locus_tag="O0V33_000732"
     CDS             25771..26865
                     /gene="rlmN"
                     /locus_tag="O0V33_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCX8975398.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            26872..27615
                     /locus_tag="O0V33_000733"
     CDS             26872..27615
                     /locus_tag="O0V33_000733"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HCX8975399.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNQTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEQHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            27612..29606
                     /gene="pknB"
                     /locus_tag="O0V33_000734"
     CDS             27612..29606
                     /gene="pknB"
                     /locus_tag="O0V33_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HCX8975400.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV"
     gene            29834..30709
                     /gene="rsgA"
                     /locus_tag="O0V33_000735"
     CDS             29834..30709
                     /gene="rsgA"
                     /locus_tag="O0V33_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCX8975401.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFDVQNINEGYIHQVYERKNELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARILVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWSAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            30710..31354
                     /locus_tag="O0V33_000736"
     CDS             30710..31354
                     /locus_tag="O0V33_000736"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCX8975402.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            31361..32002
                     /locus_tag="O0V33_000737"
     CDS             31361..32002
                     /locus_tag="O0V33_000737"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCX8975403.1"
                     /translation="MHINLLCSDRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
     gene            complement(32383..32571)
                     /locus_tag="O0V33_000738"
     CDS             complement(32383..32571)
                     /locus_tag="O0V33_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCX8975404.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
BASE COUNT        11741 a         4624 c         6124 g        10110 t
ORIGIN      
        1 tgtaaaaaat gtgtgattta ctaaaataaa aattattcag gaatggtaca aattctctga
       61 agcttataaa tgcgttatag ttgctattct caattatgtt cgcgataatt ttaagtaaaa
      121 gtaagcacag atattgaatt tgataggagt taattgaatg tatcataaca gtaacgcaaa
      181 ctttgtaaat ggtatcactt taaatgtgag agataagaat gaattaaagc cattttatga
      241 ggacgtatta ggattaaata ttataaatga gacattaaca tcgatccaat atgaagtagg
      301 tcaaaataat catgtcatta cacttgttga attacaaaat gggcgtgaac ctttaatgtc
      361 agaagcggga ctgtttcata tcgcaattaa actacctcaa attagtgatt tagctaattt
      421 actaattcat ttaagcgaat atgatattcc agttaacgga ggtatacagc ctgcttcgtt
      481 atcattattt tttgaagacc cggaaggaaa cggttttaaa ttttatgttg ataaagacga
      541 agcgcaatgg acgaggcaaa atgatttagt aaaaattgat attagaccat taaatgtacc
      601 gagattagtg agtcatgcaa caaaattgtt atggttaggt attccagatg acgctattat
      661 aggtgcattg catattaaga caattcattt atcagaggta aaagactact acctcgatta
      721 ttttggatta gagcaatcgg catatatgga tgattattca atatttttag catcgaatgg
      781 ctattatcaa catttggcca tgaatgattg ggtatcagca acgaaacgtg tagaaaattt
      841 tgatacgtat ggattagcaa ttgttgactt tcattatcct gaaacaacac atttaaattt
      901 acaaggtccg gatggtatct attatcgctt taatcatatc gaagttgaag attagtaaat
      961 actttgaatg gacgaaccat ataatgaatc gtttttaatg atctttttat acaagttatg
     1021 aaggcagtct tatgaggttt cccatgaggc tgctttcata atttttaatg tcaaaacata
     1081 gtagtttatc aagtattgag ttagtaacat tagatttaat gtaatatgct tacttttttt
     1141 attagcaggt gtaagctata atataaagag ttgtcttatg gacgattgat tggaggaacg
     1201 aaaatgcaca aaaaatattt tattggcact tccattttaa tagcagtatt tgtcgttata
     1261 tttgaccaag ttactaaata tattatagct actacaatga aaattggaga ttcatttgaa
     1321 gtgataccgc actttttaaa cataacatca catcgaaata atggtgctgc atggggaata
     1381 ttgagtggaa aaatgacatt tttctttatt attaccatta ttatattaat agccttagta
     1441 tatttcttta ttaaagatgc tcaatataat ttgtttatgc aagttgctat tagtttactt
     1501 tttgcaggtg cacttggaaa ctttattgat agagttttaa caggagaagt tgttgacttt
     1561 attgatacaa atatttttgg ttatgatttt ccaatattta atatcgcaga ttcaagttta
     1621 acaattggtg taatattaat tattattgcc ttattaaagg atacttccaa taaaaaggag
     1681 aaggaggtta agtaatggag acttatgaat ttaacattac agataaagaa caaacaggta
     1741 tgcgtgtaga taagttgctg cctgaattaa atagtgattg gtctcgtaac cagatacaag
     1801 attggattaa agcaggttta gtcgttgcaa acgataaagt tgttaaatct aattataaag
     1861 tgaaacttaa tgatcatata gttgtcactg aaaaagaagt ggttgaagct gacattctac
     1921 ctgaaaattt aaatttagat atttattatg aagatgatga cgttgcagtt gtttataaac
     1981 cgaaaggcat ggtagttcat ccatcaccag gtcattatac caatacatta gttaatggtt
     2041 taatgtatca aattaaagat ttatcgggta ttaatggaga aattcgtcca ggtattgttc
     2101 accgtataga tatggatact tctggtttat taatggttgc taaaaatgat attgctcatc
     2161 gtgggcttgt agaacaatta atggataaat ctgttaaaag aaaatatatc gctttagttc
     2221 acgggaatat tcctcatgat tacggaacaa tcgatgcgcc aattggtaga aacaaaaatg
     2281 atcgtcaatc tatggctgtt gttgatgatg gtaaggaagc agtgacacat tttaacgtac
     2341 tagaacattt taaagattat acgcttgttg aatgtcaact tgaaacagga cgtacgcatc
     2401 aaatccgtgt gcacatgaaa tatattggct tcccattagt tggtgatcca aagtatggac
     2461 cgaaaaagac attggatatt ggtggtcaag ctttacatgc tggacttatt ggattcgaac
     2521 atccagtaac aggtgaatat attgaaagac atgctgaatt agcacaagac tttgaagatt
     2581 tattagatac aattcgaaaa agagatgcat aattgtgtcg tgctataatt acgataacat
     2641 tattatgtaa actataagta tttatcgttt tcgtttttct aaaaatgaaa acactttata
     2701 aaatagatta aggttaacat ttttactgag aatggttaat ttttcaatat agattgacaa
     2761 agcgcctgat tagtattatc cttataacag tttaagtaca acataaagag tctttaattg
     2821 agcgttcaga gagacgtcaa agacatgaaa atatagatat atactaaaat gaatcataac
     2881 actttcacat atgcaagaat aagtagtaat actacgattc ttctatgtaa ataggatgat
     2941 tgtatatact aaacctcatg tcgatattaa agacatgagg ttttttgttt ctaaattttg
     3001 aaagggtgag aatatgtctg aacgtatcat aatggatgat gccgcaatac aacgtacagt
     3061 aacgagaatc gctcatgaaa ttttggagta taacaaaggt actgataatt taattctttt
     3121 aggtatcaaa acaagaggtg aatatttagc gaatcgtata caagataaaa ttcatcaaat
     3181 tgagcaacaa cgtataccta ctggaacaat tgatattaca tactttagag atgatataga
     3241 gcacatgtca tcacttacga caaaagacgc aatagacatc gacacagata ttacagataa
     3301 agtagtcatc attattgacg atgtgctgta tactggtcga acggttcgtg cttcacttga
     3361 tgctattttg ctaaatgcta gacctattaa aattggtttg gctgctttgg ttgatcgagg
     3421 acatcgtgag ttaccaattc gagcagattt tgttggtaaa aatataccta cttctaaaga
     3481 ggaaacggta agtgtctatt tagaagaaat ggatcaaaga aatgcagtta taattaaata
     3541 ataccccttt taattcagta cgagagactg aaaaaggtgg gggagttgaa tgataaataa
     3601 gttttcatat gcttgtttat ctaataatac cctcatgttc gacaactata agtcaatttc
     3661 tatggacgaa aatgacttgt agcaacttgt cacacttttt gtctctttac atatactgat
     3721 gtaaagagat ttttttatta tcggaggaaa tgaatattat gcaaaatgat gaaatgtttg
     3781 aacgaacagt aaaacctgta ctagatgtaa atgaaaaacc acaaccagcg caatgggcat
     3841 ttttaagctt acaacattta tttgcgatgt ttggcgcaac agtactagta ccattcttaa
     3901 caggattacc aatatcagca gcgttactag cttcgggaat cggtacatta ctttatatct
     3961 taataacgaa ggcgcaaata ccagcatact tgggatctag ctttgcattt atcacaccaa
     4021 ttatcacggg attaagtacg catagcttag gagacatgct tgtagcatta ttcatgagtg
     4081 gtgttatgta cgtcatcatc gggattctaa tcaaattaag tgggacagca tggttaatga
     4141 aattattacc accagttgtt gtaggaccag tcatcatggt aattggttta agcttagcgc
     4201 ctactgcagt caatatggca atgtatgaaa atcctggaga tatgaaaggt tacaatatca
     4261 gtttcttaat tgttgcaatg ataaccttgc ttgtaacaat attcgttcaa ggatttttta
     4321 aaggattcct atctttaatt ccagtacttg taggtattat cgtaggttat gtggtagcga
     4381 tttttatggg gattgtgaaa tttgatgcaa ttatgtcagc aaaatggata gatttccctc
     4441 atatttatct gccatttaaa gattatgtac cttcatttca cttaggactt gtacttgtaa
     4501 tgattccgat tgtgttcgta acagtaagtg aacatattgg gcaccaaatg gtactgaata
     4561 aaatcgtagg tagaaacttc tttgaaaagc caggacttga taaatcaatc attggtgatg
     4621 gtgtttctac aatgtttgcc agtattattg gtggaccacc aagtacaaca tatggtgaaa
     4681 atatcggtgt attagcgatt acaagaatat acagtattta cgtcattggt ggtgcggcag
     4741 ttatagcaat tgttttagca ttcattggta agttcactgc attaatttct tctataccta
     4801 caccagttat gggaggagta tctatattac ttttcggtat tattgcagca agtggcttaa
     4861 gaatgttagt tgaaagcaaa gtagattttg cgaacaatcg aaatttagtt atagcttcag
     4921 taattttagt tgtaggtatc ggtaatttag tatttaactt aaaagaaatt ggtatcaacc
     4981 ttcaaattga aggaatggca ttagctgcac tttcaggaat tattttgaac ttaatcttac
     5041 ctaaagagaa aaaacaaaac aattaagatt tacaaattaa ggagggcgct tttatgaatc
     5101 atttattatc aatggaacat ttatctacag atcaaatata caaacttatc caaaaggcaa
     5161 gtcaatttaa atctggtgaa cgtcaactac caaactttga agggaaatat gtcgcaaatt
     5221 tattctttga aaattctact cgaacaaaat gtagttttga aatggcagaa cttaagctag
     5281 ggttaaaaac gattagcttt gaaacatcaa catcatctgt ttcaaaaggt gaatctttat
     5341 atgacacatg taaaacttta gaaagtattg gctgtgattt attagtcatt agacatccgt
     5401 ttaataacta ctatgaaaaa ttagcgaata ttaacatccc aattgcgaat gctggtgatg
     5461 gtagtggaca acatccaaca caaagtttgc ttgatttaat gacgatatat gaagaatatg
     5521 gatattttga aggcttgaat gtattgattt gtggcgacat taaaaattca cgtgtcgcac
     5581 gtagtaatta ccatagttta aaagcattag gtgcaaacgt aatgtttaat agcccaaatg
     5641 cttggattga tgattcttta gaagcgcctt atgtaaatat agatgatgtt atagaaacag
     5701 tagatatagt tatgttatta agaattcaac atgaaagaca tgggcttgca gaagaaacta
     5761 gatttgcagc agatgattat catcaaaagc atggcttaaa tgaagtgcgt tataacaaat
     5821 tacaagaaca tgctattgtc atgcatccgg cacctgtgaa tagaggagta gaaatacaaa
     5881 gcgatttagt agaagcttca aaatcaagaa tttttaagca aatggaaaat ggcgtttact
     5941 taaggatggc agtcattgat gaattattaa aataggtaag gggacgaaaa tgatgaaatt
     6001 aattaaaaac ggtaaagtat tacaaaatgg tgaattacaa caagcagata ttttaattga
     6061 tggtaaggta attaaacaaa ttgcacctgc aattgaacca agcaatggta ttgacatcat
     6121 agatgcgaaa ggtcactttg tgtcacctgg atttgtcgat gttcatgttc atttacgcga
     6181 acctggtggt gaatataaag agacaattga aactggtact aaagctgctg ctagaggcgg
     6241 atttacaact gtatgtccaa tgcctaacac aagaccggta ccagattctg tagaacattt
     6301 tgaagcttta caaaaattaa tcgatgacaa tgctcaagta cgtgtattac cttatgcttc
     6361 aattacaaca cgtcaattag gtaaagaatt ggttgatttc ccagcactag taaaagaagg
     6421 tgcctttgcg tttacagatg acggtgtagg agtacaaact gcaagcatga tgtatgaagg
     6481 catgattgaa gctgcaaaag taaacaaagc catcgtagca cactgtgaag ataattcatt
     6541 aatctatggt ggtgcaatgc atgaagggaa acgcagtaaa gagttaggta taccaggtat
     6601 tccaaacatt tgtgaatctg ttcaaatcgc aagagatgta ctattagctg aagcagcagg
     6661 ttgtcattat catgtatgtc atgtttctac taaagaaagt gttagagtca ttcgtgatgc
     6721 taaacgcgca ggcattcatg ttacagctga agttacacca caccatttat tgttaacaga
     6781 agatgatatt cctggtaata atgccattta taaaatgaat ccaccattga gaagtactga
     6841 agatagagag gctttgttag aagggttact agacggtaca attgactgta tcgcaacaga
     6901 ccatgcacca catgcacgtg atgaaaaagc acaaccaatg gaaaaagcac cattcggaat
     6961 tgttggcagt gaaacagcat tcccattatt atatacgcat tttgtaaaaa atggtgattg
     7021 gacattacaa caattagtag attacttaac aattaaacca tgtgaaacat ttaatttaga
     7081 atacggcaca ttaaaagaaa atggttatgc agatttaaca atcattgatt tagatagtga
     7141 acaagaaatt aaaggagaag atttcttatc aaaagcagat aatacaccat ttatcggcta
     7201 taaagtttat ggaaatccga tcttaacaat ggttgaaggc gaagttaaat ttgaggggga
     7261 taaataatat gcaaagcaaa cgttatctag tgttagaaga cggttctttt tacgaaggct
     7321 accgtttagg atctgataac ttaactgtag gagaaattgt atttaataca gcgatgacag
     7381 gttatcaaga aactatttca gatccatcat atacaggtca gatcattact tttacgtatc
     7441 cattaatcgg taattatggt atcaatagag acgattttga atcattagta cctacattaa
     7501 acggtattgt agtgaaagaa gcgagtgcgc atccaagtaa ttttagacag caaaagacac
     7561 ttcatgacgt tttagaattg catcaaattc cagggattgc aggtgttgat acaagaagta
     7621 ttacgcgtaa aattcgacaa cacggtgtgt taaaagctgg ttttactgat cgaaaagaag
     7681 atattgatca acttgtcaaa catttacaac aagtagaatt acctaaaaac gaagtagaaa
     7741 tcgtttcgac taaaacaccg tatgtttcga caggtaagga tctaagtgtc gtacttgtag
     7801 actttggtaa gaagcaaaat attgttcgag aattaaacgt cagaggttgt aacgtcacag
     7861 ttgtaccata tacaactact gccgaagaaa ttttagcaat ggctccagat ggcgttatgc
     7921 tatcaaacgg accaggtaat cctgaagttg tagaatgtgc gattctaatg attcaaggaa
     7981 ttttagggaa aattccgttc tttggtatct gtctaggaca tcaacttttt gcattatctc
     8041 aaggagcaag ctcatttaaa atgaagtttg gtcatcgtgg tgcgaaccat ccagttaaaa
     8101 atttagagac tggaaaagtt gatattacga gtcaaaacca tggatatgca atagatatag
     8161 attcgttaaa aagtactgat ttagaagtta ctcatcttgc attaaatgat ggtactgtag
     8221 aaggtttaaa acataaaaca ttaccagcat tttctgttca ataccatcct gaagcaaatc
     8281 caggaccgtc agattcaaac tatctatttg atgattttgt agcaatgatg actaatttta
     8341 aggaaaagga gcgtcatatc aatgcctaaa cgtaatgata tcaaaacaat tttagtaata
     8401 gggtctgggc caattatcat aggtcaagca gctgaatttg attatgctgg aacacaagca
     8461 tgtctagctt taaaagaaga gggataccga gttattcttg taaattcaaa tccagcgaca
     8521 atcatgactg ataaggaaat tgcggataaa gtatatatcg aaccgttaac tcataatttt
     8581 atagcgcgaa ttatacgtaa agagcaacct gacgctttac ttccaacttt aggtggtcaa
     8641 acaggtttaa acatggcgat tcaactacac gaaagtggtg tgcttcaaga taataacgtc
     8701 caattattag gaactgagct aacatcaatt caacaagcag aagaccgtga aatgtttaga
     8761 acattaatga atgatttaaa cgttcctgtg ccagagagtg acattgtaaa tacagtagag
     8821 caagccttta aattcaaaga gcaagtggga tacccgctaa ttgttagacc agcatttacg
     8881 atgggtggta ccggaggcgg tatttgtcat aatgatgaag aattacatga aatcgtttca
     8941 aatggtcttc attatagtcc agcaacgcaa tgtttattag aaaaatctat cgcaggtttt
     9001 aaagaaatcg aatacgaagt aatgcgtgat aaaaacgata atgccatcgt tgtatgtaac
     9061 atggaaaata ttgatccagt tggtattcat acaggcgatt caattgttgt agctcctagc
     9121 caaacattat cggatgttga gtatcaaatg ttacgtgatg tttcattaaa agttattcga
     9181 gcattaggta tcgaaggtgg ttgtaatgtt caattagcat tagatcccca ttcattcgat
     9241 tattatatta tagaagtaaa tccgcgtgta tcacgttcat cagcgttagc ttcaaaagca
     9301 acaggatatc ctattgcaaa attagctgct aaaatcgcgg ttggtctaac attagatgaa
     9361 atgttaaatc caattacagg aacatcttat gcagcgtttg aaccaacttt agactatgtg
     9421 atttcaaaaa taccaagatt tccttttgat aaatttgaaa aaggagaacg agagcttggc
     9481 acacaaatga aagcaacagg tgaagttatg gccattggtc gaacttacga agaatcattg
     9541 ttaaaagcaa ttcgatcact tgagtatggt gtgcatcact taggattacc aaatggtgaa
     9601 agcttcgatc ttgattatat taaagaacgt atttcacacc aagatgatga acgattattt
     9661 ttcatcggcg aagcaattag aagaggcaca acattagaag aaattcataa tatgactcag
     9721 atagattact tcttcttaca caagttccaa aacattattg atattgagca tcaattaaaa
     9781 gagcatcaag gtgatttaga atatcttaaa tatgcaaaag attatggatt tagtgataaa
     9841 acaatagcgc atcgctttaa tatgacggaa gaagaagtat atcaattgcg tatggaaaat
     9901 gatattaaac ctgtttacaa gatggttgat acttgcgcag ctgaatttga atcttcaaca
     9961 ccatattatt atggtacata cgaaactgaa aatgaatcca tagttactga caaagaaaaa
    10021 atcttagtat taggctctgg accaattcga atcggccaag gtgtagaatt tgactatgcg
    10081 acagttcacg ccgtttgggc aattcaaaaa gcagggtacg aagcgataat tgtgaataac
    10141 aatccagaaa cagtttcaac agacttctca atttctgaca aattatactt tgaaccttta
    10201 actgaagaag atgtgatgaa tatcattaat ttagaaaaac ctaaaggtgt cgttgtacaa
    10261 tttggaggac aaacagcgat taatttagca gacaaattgg ctaaacatgg tgttaaaata
    10321 cttggtactt cactagaaaa tctaaatcgt gctgaagata gaaaagaatt tgaagcacta
    10381 ttaagaaaaa ttaacgtgcc acagccacaa gggaaaacag ctacatcacc tgaggaagca
    10441 ttagcgaatg ctgcagaaat cggatatccg gttgtagtaa gaccttctta tgtattaggt
    10501 ggtcgcgcaa tggaaattgt agacaatgac aaagagttag aaaactatat gacccaggct
    10561 gtaaaagcga gtccggaaca tccggtatta gtcgatagat atttaactgg taaagaaatt
    10621 gaagttgatg cgatttgtga tggagaaacg gtcattattc caggaattat ggaacatatt
    10681 gaacgtgctg gtgtgcatag tggtgactca atcgctgtat atccgccaca aactttgaca
    10741 gaagacgagt tagcaacgct tgaggactat actataaaat tagctaaagg tttaaacatc
    10801 attggcttaa tcaacattca attcgttata gctcacgatg gtgtgtatgt tttagaagta
    10861 aatccacgtt ctagtagaac ggtaccattc ttaagtaaaa ttactgatat tccaatggca
    10921 caattagcta tgcgagcaat cattggggaa aaactaacag atatgggtta tcaagaaggg
    10981 gttcaaccat atgctgaggg tgtatttgtg aaagcaccgg tatttagttt taataaattg
    11041 aaaaatgttg atattacttt aggacctgaa atgaagtcta caggtgaagt aatggggaaa
    11101 gatacaacat tagaaaaggc gttattcaaa gggttaacag gtagtggtgt tgaagttaaa
    11161 gatcacggta cagtattaat gactgtcagt gacaaagata aagaggaagt tgttaaattg
    11221 gcacaatgct taaatgaagt tggctataaa attttagcaa cgtctggaac agctaataaa
    11281 ttagctgagt atgacatacc tgcagaagta gtaggcaaaa ttggtggcga aaatgattta
    11341 ttaacacgta ttcaaaatgg tgatgttcaa atcgttataa atacaatgac taaaggtaaa
    11401 gaagtagaaa gagatggctt ccaaattaga cgtactacag ttgaaaatgg tattccatgt
    11461 ttgacatctt tagatacagc taatgcctta acgaatgtaa ttgaaagtat gacatttaca
    11521 atgcgtcaaa tgtaaatcaa tcaaactgta tcggtggggc tgtaattaac catttactta
    11581 aagaagttta tattacagcc tcattatttt agtgaatttc ttaacgtaaa gggagacata
    11641 tatgatgaaa gatttaccaa ttattgcatt agattttgaa tcaaaagaaa aagtaaatca
    11701 atttttagat ttatttgatg aatcattatt cgtaaaagta ggtatggaac ttttttatca
    11761 agaaggtcct caattaatta atgagataaa agaaagaggc catgatgtat ttttagattt
    11821 aaaactgcat gatattccca atacagttgg taaagcgatg gaaggactag ctaaattgaa
    11881 tgttgatctg gtaaatgttc atgctgctgg tggcgtaaaa atgatgtctg aggccattaa
    11941 aggattaaga aaacataatc aacatacaaa aattattgca gtaacacagc ttacgtcaac
    12001 aacagaagac atgttacgac acgaacaaaa tatacaaaca tcgattgaag aggccgtttt
    12061 aaattatgcc aagttagcaa atgcagctgg tttagatggc gttgtttgtt cacctcttga
    12121 aagtcgtatg ttgactgaaa agttaggtac atcattttta aaagtaacac caggcattag
    12181 acctaaaggt gcatctcaag atgaccaaca ccgtattacg acaccggaag aagcaagaca
    12241 gcttggttcg acgcatattg tagtcggtag accgattaca caaagtgaca atccagtcga
    12301 aagttatcat aaaattaaag aaagttggtt agtataatgg ctaaagaaat tgcaaaatca
    12361 ttattagata ttgaagctgt aacattatca ccaaatgatt tatatacatg gagttcaggt
    12421 attaaatcac cgatttactg tgataaccgt gttacgttag gttatccttt agttcgaggc
    12481 gcaatccgcg atggtttaat taacttaatt aaagaacact ttcctgaagt agaagttatt
    12541 tctggtactg caacagctgg tattccacat gcagctttta ttgctgaaaa attaaaatta
    12601 ccaatgaatt atgttcgttc atcaaataag agtcatggta agcaaaatca aattgaaggt
    12661 gctaaaagtg aaggtaaaaa agtagttgtg atagaagatt taatttcgac agggggatct
    12721 tcagtcacag cagttgaagc cttaaaacaa gcaggcgcag aagtattagg tgttgttgct
    12781 atctttactt acggtttgaa aaaagcagat gatacattta gcaatattca actacctttt
    12841 tacactttaa gtgattacaa tgaattaatt gaagtagctg aaaatgaagg taaaatttct
    12901 agtgaagata tccaaacatt agttgaatgg agagacaact tagcataata tagacgctag
    12961 aaggaggaat tcaacaaatg aatgacaaaa catctaatga tttatatggg aagataaaac
    13021 attgtaacga atttatcaat cattcaaatg attccaatct atctagtagt cacgatgtcg
    13081 acgaaagttc aacgaagcaa aaacatataa aaaataaaac aactataaat cataatgatg
    13141 atttatttaa acatgtaaag gatatattac gtaaacaagg acaaatttaa aaacaaaaaa
    13201 gctatagtgt ttaatatcag gggctgctta accgcatccg aatattacaa acactatagc
    13261 ttttattata taaaaaattt attagcggat aattaacttt attaaaatcc aaccaattaa
    13321 gaatacgagt aataggacta atactggaat cacataatgt aacataacgt ccctccttta
    13381 acttaatttt aattgtaatc aaatttgaca ataagtcaaa acattaatac ctatgataag
    13441 tatcatttat taacatatgt atcatatttt taatcttgcg taatttttat cgttaactat
    13501 gtgatttaat caacaggcca ataagacatt tgtcattcgt gcacataacg gtacgacgcg
    13561 cttttgtcat tttaattatg ttaagataat agtagttata gaagttcaat ctataggagg
    13621 catagcatgg atattccaaa aattacgaca tttttaatgt ttaataacca agctgaagaa
    13681 gctgttaaac tatacacaag cttatttgaa gatagtgaga ttataacaat ggctaagtat
    13741 ggtgaaaatg gacctggtga tcctgggact gtacaacact caatatttac attaaatgga
    13801 caagtattca tggcgattga tgctaatagt ggcacagaat taccaatgaa tcctgcgatt
    13861 tcattatttg ttacagtaaa agatactatt gaaatggaac gactatttaa tggattaaaa
    13921 gatgaaggtg ccattttaat gccaaaaacg aatatgccac catacagaga gtttgcttgg
    13981 gttcaagata agtttggagt aagttttcaa ttagcattac ctgagtaaaa ggattcgtac
    14041 agcgttgaat gataaagaaa cactttttct tatgcatgcg ttttacctat gtattcctat
    14101 tttaagtaca cattatcatt ttagtttcac tcatttttaa aatcacaaag ataattttta
    14161 acttaaatga tgatacgtta ttaaaaaaca cgatacttcg tttcaatgaa cgcatcaaat
    14221 taaaaataaa cacctcgcaa aagaaagtag gcctttctta tggtgaggtg tttatttgtc
    14281 gacaacttga ttatgattgt ttcatttttt gaatatgttc ataatcaggt gtagcataca
    14341 aagttttttg attgtcatat gttacaaacc caggctttgc acctgatggt ttgtgcacat
    14401 ttttaattaa cgtgtaatca acaggtattt gtccagaatt accagctttt gaaaagtatc
    14461 ctgctaacat agccgcttcc ttgattgtcg tatcacttgg tgcatcatta aatataacga
    14521 catgtgaacc aggaatatct tttgtgtgta accatgtgtg agttttttta gcttttttat
    14581 ttgttaaata atcattttgc ttgttattct tgccaacata tatatcgtcg ccatcagttg
    14641 atacataatg ttgtaattga atctgcgctt tctttttctt agtttgattt ttacgctgtt
    14701 tcataaagcc ttgttctgct aattcatctc taatttcatc aatgtcatgg acagaaatat
    14761 gatgtaattg ttgttcgatt gttgaaaaat aatctatatt gtctttcgtc aattgaattt
    14821 gatgttgtaa ttcacgttct ctcgttttca tacgattata ttgtttataa taatattgag
    14881 catttgcaga tggggatttt gtaggattta aaggaatgac aacttcttca ttcgtataat
    14941 aattcaatgc cgtcacttct ttatcgcctt gcttaattcg atatatatta gcagtaatca
    15001 attcaccata taactgttca gtatctttat ttttagactg ctcatattct tcaatcaact
    15061 tcgctaattt attttgatat ttgtgcaact gctgttgaac aaatcgaact aaatcattcg
    15121 cacgttgttt aacgcgttcc cgttcgccac gcgcatcata aaaacgatca agtaaatcat
    15181 ttaatgaatc gtacgtaact gtatcatcat taaattgatt taactttata aaatagaaat
    15241 cctctttacc tgtttcatga tttttatgaa aaataggagt aggtggtaac ttggtttctg
    15301 ccattacttc gtcgaatgct tctggtaaag ttgatgaagt cataaattga cgacgattaa
    15361 cgatttcatt cgtaattaaa gggctaaacc cttcaaactg attcaataat tgtttagcaa
    15421 tattacctgc gttaaaatcg atatatttca acacctctgc acctgtaata tcatacggat
    15481 tcattttgtg ctgagtaggt ggtgcttcat aattaaatcc tggcattact gttcgatagt
    15541 gattcgtatt tggtgttaag tgtttaaatc cttcaattat tttacgattt tcatctacta
    15601 aaattaagtt actatgttta cccataatct caaggatgac agtgcggtaa atagtatcgc
    15661 caatttcatc tttactcttt atatcgattt caatgcgacg atcattacca atttgcttaa
    15721 tcgattcgat aataccacct tctaagtgtt ttctaaaaac acgcgcaaac atgggtggat
    15781 taaatggatt atcatatttt ttagtagtca attgtaatct tgaaaagttt ggatggattg
    15841 acaataacaa ttgatggttt tgtctatttt gacgtacaac cattagtatc gtgtcattat
    15901 caggttgatt gattttgtga acgcgtcctg ttgttaaaaa ttgtagagac tcaaccattt
    15961 tctttgtaaa taagccatca taagccataa tttatcttcc accttcttca tatcattcgt
    16021 ttcattttaa catgattgta acttttcggt atagttattg aatcatacat tatgccacac
    16081 atttaacatt atctaaaagt tcgcaattca atttatttaa ttaaaattaa tcgtgcaata
    16141 taacgatatt gttagaccta atagaaatta tggcatgcaa tttcaaatat gctatacaat
    16201 ataaagaaca atgtgatatc atatttaaat aatagaagat tagcttagag aggtcgtaag
    16261 gcatggataa tgaaaaagga ttgttaatcg ttttatcagg accatctgga gtaggtaaag
    16321 gtactgttag aaaacgaata tttgaagatc caagtacatc atataagtat tctatttcaa
    16381 tgacaacacg tcaaatgcgt gaaggtgaag ttgatggcgt agattacttt tttaaaacta
    16441 gggatgcgtt tgaagcttta attaaagatg accaatttat agaatatgct gaatatgtag
    16501 gcaactatta tggtacacca gttcaatatg ttaaagatac aatggacgaa ggtcatgatg
    16561 tatttttaga aattgaagta gaaggtgcaa agcaagttag aaagaaattt ccagatgcgt
    16621 tatttatttt cttagcacct ccaagtttag atcacttgag agagcgatta gtaggtagag
    16681 gaacagaatc tgatgagaaa atacaaagtc gtattaacga agcacgtaaa gaagtcgaaa
    16741 tgatgaattt atacgattac gttgtagtta atgatgaagt agaacttgcg aagaatagaa
    16801 ttcaatgtat tgtagaagct gagcacttaa aaagagagcg cgtagaagct aagtatagaa
    16861 aaatgatttt ggaggctaaa aaataatgtt aaatccacca ttaaaccaat taacgtcaca
    16921 aattaaatca aagtatttaa ttgcaacaac tgcagcgaaa agagcgcgtg aaattgatga
    16981 acaacctgaa actgaattat taagtgaata tcattcattt aaaccagttg gtagagcgtt
    17041 agaagaaatt gctgacggta aaattcgccc tgttatttca agcgattatt atggtaaaga
    17101 atagtttcga ccattaaaaa tatgtgaagc aagacgtata tcaaatacgt caaggcatat
    17161 ccaaatcaaa cgcacctatg taggtgtgtt tttttagttt atccaatgat tgatgttata
    17221 ataatactaa atttgtatct ataaaaaagt aatgagcatt tgtgcgcata tgatgatgta
    17281 aagcgtaaat gtcatagtga tatttaaggg agattgaata tgaagaaaat attgttagcc
    17341 gttacaggtg gcattgcggc atataaagcg attgatttga caagtaagtt aacacaatct
    17401 gggtatgaag ttcgcgttat gttaacgaat cacgcacaaa aatttgtgac accattagca
    17461 tttcaagcaa taagtcgaaa tgctgtttat acagatactt ttatagaaga aaatccttca
    17521 gaaatacagc atatagcatt aggtgattgg gcagatgcaa tcattgttgc acctgcaacg
    17581 gcaaatacaa ttgcaaaatt gagtgtaggt attgctgatg atttggtgac atcaacgttg
    17641 ctagcaacag agacaccgaa atttattgcg cctgctatga atgtgcatat gtatgaaaat
    17701 aaacgtacgc agcaaaatat taatatttta aaagaagatg ggtatcattt tatcgaacca
    17761 ggaagcggat ttttagcatg tggttatgtt gctaaaggac gtatggaaga accgcttcaa
    17821 atcgtttctg ttattgatgc tcattttcaa aatagtaatc gtttagctaa tagttcattt
    17881 caagataaac gcgcattggt tacagccgga ccaactattg aagttatcga tccagtcaga
    17941 tttgtatcca atcgttcttc tggaaaaatg ggttatgcaa tagctgaagc attgcgaaat
    18001 cgaggagcta tcgtgacatt agttgcgggg cctacaacac tagaggatcc aaaagatatt
    18061 gaagttattc atgttcaaag tgctgaagaa atgtttgaac aagtgacaag ccgatttgac
    18121 gaacaagata ttgttgtaaa agcagcagcc gtatctgact atacaccggt tgatgtatta
    18181 gaacataaga tgaaaaagca ggacggtgat ttgtcagtat cttttaaacg tactaaagac
    18241 attcttaaat atttaggtga acataaaaca tcacagtatt taataggctt tgcagcagag
    18301 actgaagata ttgaaaatta tgcacaacaa aaattacgca agaaaaatgc agatgtgatt
    18361 atttcaaata atgttggaga tatgtctatc ggatttagtt ctgatgataa tgaattgaca
    18421 atgcatttta aaaataatga aaaagtaaat atcaagaaag gaaaaaaagt agtattagct
    18481 gcacaaattt tagatgaact agaaactagg tggcaataat gatagcgaaa gtcatagtcg
    18541 atgtcgcgtc gaagagcgtt gactataaat ttgattatat aattcccgaa caactcgaat
    18601 ctgtcatcca acctggtgtg cgtgtgattg taccttttgg accaagaacg attcaaggtt
    18661 atgtaatgga agtaacagca gaacctgatg cacaacttga cgtttcgaag ttaaaaaaaa
    18721 tcatagaagt gaaagatata caaccagaat taacatcaga attaatagct ttaagtgagt
    18781 ggatgggttc aactcatgtc attaaacgta tttctatgct agaagtgatg cttccgagtg
    18841 ctattaaagc gaagtataaa aaagcattta agatgaaaga tgacatagag gtaccttcaa
    18901 ctttattaca aaaatttgat aagcatggtt actattatta taaagatgcg caaaaaaata
    18961 atgatattca attgcttatg aagttgttaa aagatgatat cgttgaagaa agaacgattc
    19021 tcacacaaaa tataactaaa aaaaccaagc gtgctgttcg tgtcattgaa gggtatcatc
    19081 ctgatgaagt attagctaag ttggagaaag ttattaaaca atatgatttg tatgcttact
    19141 tgtctgaaga acaacataaa acaatatttt taactgatat tgaggatatg ggcttttcaa
    19201 aatctagttt agatggactt atcaaaaaag gttatgttga aaaatatgac gcggttgttg
    19261 aaagagaccc atttaaagat cgtgttttcg aacaagaatc aaaacagcaa ttaacagaag
    19321 accaatataa agcatatgaa gcgattaaag ctaaaattgt aagccaagag caagaaacat
    19381 ttttacttca tggtgtgacg ggatcaggta aaacagaagt atatttacaa acgatagaag
    19441 atgttttaag tcaaggaaaa caggcgatga tgttagttcc tgaaatcgct ctaacaccgc
    19501 aaatggtttt acgcttcaaa cgtcgatttg gtgatgacgt tgctgtatta cattctggct
    19561 tatctaatgg ggaacgttat gatgagtggc aaaaaattag ggatggtcgt gcgagagtaa
    19621 gtgttggtgc aaggtcaagt gtgttcgcac ctttcaaaaa tttagggtta atcatcattg
    19681 atgaagaaca tgaatctaca tataagcaag aagattatcc gagatatcac gctagagaaa
    19741 ttgcccaatg gcgaagtgaa tatcatcact gcccagtcat tttaggaagt gcaacaccat
    19801 gtcttgaaag ttatgcacga gctgaaaaag gtgtttatca tttgctatca ttaccaaaca
    19861 gagtgaacca acaagcttta cctgaaattg atatcgtaga catgcgtgaa gaattgagtg
    19921 aaggtaatcg gtcaatgttt tcaaaagatt tacgtgaagc catacaatta agattagatc
    19981 gacaggaaca agttgtttta tttttaaatc gacgtggtta tgcatcattt atgttatgtc
    20041 gggattgtgg atatgtaccg caatgcccaa actgtgatat ttcattaacg tatcataaaa
    20101 cgacagactt attaaaatgt cactattgtg gttaccaaga gacaccaccg aatcaatgtc
    20161 caaattgtga gagtgaacac attcgacaag taggtactgg tactcagaaa gttgaagaac
    20221 tattgcaaca agaatttgaa gatgcgcgca taattaggat ggatgtagat acaacctcaa
    20281 agaaaggtgc acatgaaaag ttattgactg aattcgaaaa aggtaacggt gacattttac
    20341 taggtactca gatgattgcg aaaggattag attatccaaa cattacttta gttggtgtgc
    20401 tgaatgcaga tacaatgtta aatttacctg attttcgggc gagcgaacgt acttatcaac
    20461 tattaacgca agtggctggt agagctggtc gtcatgaaaa ggcaggtcaa gtcatcattc
    20521 aaacgtataa tccagatcat tattcaatat tggatgttca aaaaaatgat tatttaacat
    20581 tttatcgtca ggaaatggaa tatcgtaaat taggaaagta tccaccgtat tattatttga
    20641 ttaatttcac aatctcacat aaagaaatga agaaggttat ggaagcatcg caacatgttc
    20701 ataaaatttt attacagcat ttaacagaaa aagcgcttgt actaggtcca tctccggcag
    20761 cacttgcgag aatcaacaat gaatttagat tccaaatttt agtgaaatat aaaagtgaac
    20821 ctggattatt acaagccatt cagtttttag atgactatta ccatgaaaaa tttataaaag
    20881 aaaaattagc gttgaagatt gatattgatc cacagatgat gatgtaattt tttgactaaa
    20941 acactttaat accaatgttt cctaagagtt tgaaaggtct tgtttttact ttaagggcag
    21001 aaaaagggca gaatatacaa ctgttaacca tttaaattag cagtttatat tctgtctttt
    21061 tatatggctt tataacttac gtgattttga tttgattagg gatttattag tattttcatt
    21121 tacgaattct gattttaatg taagtgccat acttactgat aaataaaagt actaatgatt
    21181 atgcaaacta tttttaaatg gcaaattgaa atggtgaaaa tgttacactt gtaatttaat
    21241 ggctataaac tattttatat ctatctatgt tttaactact taaattatta gacaaaacaa
    21301 ttttcttaaa ttatcaatta aagtatttaa aaatattgat ggttaatgta aaatatcaat
    21361 ataaataagt tttttaaatt tatgtatgtt tatttgattc aaacaaaata acttaagagg
    21421 agaaagttta tgaaaaagac actgggatgt ttacttttaa ttatgctttt agtcgtagca
    21481 ggttgttctt ttggtgggaa tcataaatta tcatcaaaga aatcagaaga atcaaaacaa
    21541 gaaactgtaa aaaaagaatc ggaagaagag aaagatccag atttagagaa atttgaagaa
    21601 atagagaaga aaatgaaagg aattaaagat gcgccatctc tagataagtt ggatccatta
    21661 atgacagaaa agtcgtttac gaatagtaaa gggattcaag gatggaaaga ttacaaagaa
    21721 ttaatgggta aagtggaact tgcagattat agatttacaa aagattcaaa aggatcttct
    21781 ataaaagatg ttgatgcatt ctttaaaggt aagaaaggta taaaaaggaa agtgattgaa
    21841 acacacgatg atgtaaaaca agttgattat tggtatgtag atccagatgg aaagaaaatt
    21901 ggcaattcaa acacacctgt tttttacgca gaaattatga caaaatataa agatggaaag
    21961 ttagtttatg catcagtcga accaggttct tacgtaatac ataaagatga tgcaattaaa
    22021 tatgacgatt attctaagtt aaaaaaatta agtcaactaa ctaaacttga tcatccaaag
    22081 ccagttccat atagcgtagc tcaaatcaaa tctttcggag tacctttaac aagcgtttca
    22141 tttatgacgc atggatcaaa ggatactaaa gatgaagtga tgccggcatt ggcatatttc
    22201 actttttcac caaaaaatta tgaagacaag tctaatccag atccaaaagt tttaaattta
    22261 gtagggatgg atttcttaaa tgcatctagt gattttggta acgcacattt tgttgttcta
    22321 agtaaatata ttaaagagta cgaatcaaac tatgaaacag cgtcagatga ttctttaaaa
    22381 tagtatttac tgtgcggaaa ataaatagtg tgctacatta aataatcgca ataataatcc
    22441 cgataaacaa tcagcattgc tgcttatcat atagagttcg taataactat aactctatga
    22501 ttcgcaaata ataaatgatt gtcatcggga tttattttta tcaatttata aagtgacatt
    22561 accttgttca tcagcaggtt tgaaaacagt aatcactgca ctaataattg ctaaaatgtg
    22621 tgggatacct gtccaacaga atattaagtg tagaatacct tgcatattct tgccggcata
    22681 aaatttatga ataccaaaac tacctaagaa caatgctaat aaaatataaa taactttgtt
    22741 tacttgcatt tctttccctc cagttgaatt gcttataatg acattagctt ctctttttat
    22801 tatacccact tttagttcaa acattctagt ttcagcattc ccaatcatct aaatttcatt
    22861 ttttcaatcc ttacaataaa tttaggattt catttcagtt gcattgtatt attttacgtg
    22921 tgaaatatac gtaatgaatc acattacaat ctttaaattg aaaaatatac attctatgat
    22981 gtaaggtcgc atttttaata tatttacgtt ataatggttt gatgaatttt attgaggagt
    23041 aagagctatg gcgattaaaa agttagtacc agcatcgcat cctattttaa cgaaaaaagc
    23101 gcaagcagtt ataaaatttg atgattcgtt aaaaagatta ttacaagatt tagaagatac
    23161 aatgtatgca caagaagctg ctggcttatg tgcacctcaa attaatcagt cattgcaagt
    23221 ggcaatcatt gatatggaaa tggaaggatt attacaactt gttaatccga aaattattag
    23281 tcaatcaaat gaaacgataa cagacttaga aggttcaatt acattgccag atgtttacgg
    23341 cgaagtgaca agaagtaaaa tgatcgttgt cgaaagttat gacgtcaatg ggaacaaagt
    23401 tgaactaact gcacatgaag atgtagcaag aatgattttg catattatag atcaaatgaa
    23461 cggtatccct tttacagaac gtgcggaccg tattttaaca gataaagaag tggaggcata
    23521 ttttataaat gactaaaata atatttatgg gtacaccaga cttttcaaca actgttttag
    23581 aaatgcttat tgcagaacat gatgtcattg cagtcgtaac gcaaccagat cgacctgttg
    23641 gacgtaaacg tgttatgaca ccaccaccag ttaaaaaagt tgcaatgaaa tatgatttac
    23701 ctgtatatca gcctgaaaaa ttaagtggat cagaagaatt agaacaattg cttcaattag
    23761 atgtagattt aattgtaaca gccgcttttg gacaattatt acctgaatca ttgttggcat
    23821 taccaaaact tggcgcaatt aatgtacatg catcattgtt gccgaagtat agaggtggtg
    23881 caccaattca tcaggcaatt atcgatggtg aacaagaaac cggcataaca attatgtata
    23941 tggttaaaaa attagatgcg ggtaatatta tttcgcaaca agcaattaaa atagaagaaa
    24001 atgataatgt cggtacgatg catgataaat taagtgtatt aggagcagat ttattaaaag
    24061 aaactttacc atcaattata gagggtacaa atgaaagtgt accacaagat gatacgcaag
    24121 caacatttgc ttccaatatt cgacgcgaag atgagcgaat tagctggaat aaaccaggaa
    24181 gacaagtgtt taatcaaatt cgaggattat caccatggcc agttgcttat acaactatgg
    24241 atgacactaa cttgaaaata tacgatgctg aactcgttga gactaataag ataaacgagc
    24301 ctggaacaat tatagaaacg actaaaaaag ccattattgt tgctacaaat gataatgaag
    24361 ctgttgcaat taaagatatg caattggctg ggaaaaagag aatgttagct gccaattatt
    24421 taagtggtgc gcaaaacaca ctagtaggga agaaacttat atgatagaaa acgtgagaag
    24481 tcttgctttt gacacgattc aagatatatt aaatgaaggt gcgtatagta acttgcgtat
    24541 caatgaagtg ttgtcagaaa atgaattaaa tgtaatggat aaagctttat ttacagaaat
    24601 tgtctacgga accgttaaaa gaaaatatac gttagatttt tatttaaagc cttttgtgaa
    24661 aacaaagatt aaggcatggg ttaggcaatt attatggatg agtatttatc aatatgttta
    24721 tttagataaa gttccaaatc acgccatcat taatgaagca gttgaaatag caaaagaacg
    24781 cggtggctat cacaacggaa atgtcgtaaa tggcatttta cgaacaatga tgcgtagtga
    24841 cttacctgat tttaatgaaa ttgcagatcc taaaaaaaga atggcaatcg agtatagtat
    24901 gccgaagtgg attatagatc attgggcaac acattatggt ctcgaagaaa ctgaaacgat
    24961 tttacagtca tttttagaaa cgacatcaac aactgtgcgt gccaacctga cgcgagcatc
    25021 attagatgat attattgaaa agttgcaaga cgaaggttat gacgttgaaa aagatcatga
    25081 cttaccttat tgtctccata taggaggaca accaattatt cattctcgtt catttaaaga
    25141 tggattcgtt tcaattcaag ataaaagctc aatgtttgtt gcacacatta tgaatgtaga
    25201 ccgacatgat cacgtattag atgcatgtag tgcacctggc ggtaaggctt gtcatattgc
    25261 tgaagtttta atgccagaag ggcaagttga cgcttcagat atacatgatc acaaaataga
    25321 cttaattaat tttaatataa aaaaattacg attaacaaat attaaagctt ttcaacatga
    25381 tgcgacaaaa ccttatgata aaacatacga taagatactt gttgatgcac catgtagcgg
    25441 attaggtgtt atgagacaca agccggagat taaatatact caaagcaaac aacatattga
    25501 gtcactagtt gatttacagc ttgaaatatt ggaaaatgta aaaaacaatg taaaaatagg
    25561 tggcgaaatc atctattcca catgtacaat agagcaacta gaaaatgaaa acgtgattta
    25621 tacgtttttg aaaaataata aaaacttcga atttgaaccg tttcaacatc cgataactgg
    25681 agagttggtc aaaacgttac aaatcatgcc gcaagacttt aattcagatg gattctttat
    25741 cactaagata aaaagaaagg acaattagga atgataactg ctgaaaagaa aaagaagaat
    25801 aaatttcttc caaattttga caagcaatca atatattcat tgcgatttga cgaaatgcaa
    25861 aactggctcg ttgaacaagg tcaacaaaaa tttcgagcga aacagatttt tgaatggtta
    25921 tatcaaaaaa gagtagattc gattgatgaa atgacgaact tatcgaaaga cttacgacag
    25981 cttttaaaag ataactttac tgttacaact ttaacaactg tagtaaaaca agaaagtaaa
    26041 gacggtacaa ttaaattctt atttgaatta caagatggct atacaattga aactgtttta
    26101 atgagacatg attatggaaa ttcagtatgt gtaacgacac aagtaggttg tcgtatcgga
    26161 tgtacgtttt gtgcttctac acttggcggc ttaaaaagaa accttgaagc tggcgaaatt
    26221 gtttcacaag ttttaacagt tcaaaaagcc cttgatgcta cagaagagcg cgtatctcaa
    26281 attgtcataa tgggtatcgg tgaaccattt gaaaattatg atgaaatgat ggacttttta
    26341 agaatcgtca atgatgataa cagtttaaat attggtgcac gtcacattac agtatcaaca
    26401 tcaggtatca ttcctagaat atatgacttt gcggacgaag atattcaaat taattttgct
    26461 gtaagcttac acgccgcaaa agatgaagtg cgatcacgct tgatgccaat taaccgtgca
    26521 tataatgttg agaagttaat cgaagcaatt caatattatc aagaaaaaac aaatcgtcgt
    26581 gttacttttg aatatggtct gtttggtggt gtgaatgacc aactagaaca tgcaagagaa
    26641 ttagcacatt taataaaagg cttaaactgc catgttaact taattcctgt caaccatgtt
    26701 ccagaaagaa attatgtaaa aacggctaaa aatgatatct ttaaatttga aaaagaatta
    26761 aagagactag gaattaatgc cacaatacgt cgtgaacaag gttcagatat tgacgcagct
    26821 tgtggtcaat taagagcaaa ggaacgacaa gtagaaacga ggtaaagaca aatgctagag
    26881 gcacaatttt ttactgatac tggacaacat agagataaga atgaagatgc gggtggtatt
    26941 ttttataatc aaactaatca acaactttta gttctgtgtg atggtatggg cggccataaa
    27001 gcaggagaag ttgcaagtaa atttgttaca gatgagttga aatcccgttt tgaagcggaa
    27061 aatcttatag aacaacatca agctgaaaat tggttgcgta ataatataaa agatataaat
    27121 tttcagttat atcactatgc acaagaaaat gcagaatata aaggtatggg tacaacatgt
    27181 gtttgtgcac ttgtttttga aaaatcagtt gtgatagcaa atgtcggtga ttctagagcc
    27241 tatgttatta atagtcgaca aattgaacaa attactagtg atcactcgtt tgttaatcat
    27301 cttgttttaa cgggtcaaat tacgccggaa gaagcattta cacatccaca acgtaatatt
    27361 attacgaagg tgatgggcac agataaacgt gtgagtccag atttgtttat taagcgatta
    27421 aatttttatg attatttatt attaaattca gatggattaa ctgattatgt taaagacaat
    27481 gaaattaagc gtttgttagt aaaagaaggt acaatagaag atcatggtga tcaattaatg
    27541 caattggcat tagataacca ttcgaaagat aacgttactt tcatactcgc ggctattgaa
    27601 ggtgataaag tatgataggt aaaataataa atgaacgata taaaattgta gataagcttg
    27661 gcggcggtgg catgagtacc gtttatcttg cagaagatac gatacttaac attaaagttg
    27721 caattaaggc gatttttata ccacctagag aaaaagaaga aacattaaaa cgttttgaac
    27781 gagaagtaca taactcatca cagctatcac atcaaaatat agtaagtatg atcgatgttg
    27841 atgaagaaga tgactgttac tacttagtaa tggaatatat tgaaggtccg actttgtctg
    27901 agtatattga aagtcatggg ccattaagtg ttgacacggc gattaatttt acgaatcaaa
    27961 tattggatgg cattaaacat gcgcatgata tgcgtattgt acatagagat attaagccac
    28021 aaaatatatt aattgacagc aataaaacgt tgaaaatatt tgattttgga attgctaaag
    28081 ctttaagtga gacgtcttta actcagacta atcatgtgtt aggtactgtg cagtactttt
    28141 cgccagaaca agcaaaaggt gaggcaacgg atgaatgtac agatatttat tctataggta
    28201 ttgtgttata tgaaatgctt gttggtgaac caccatttaa tggagaaact gcagttagca
    28261 ttgcgattaa acatattcag gattctgtgc caaatgtgac aaccgatgta cgtaaggata
    28321 ttccgcaatc tttaagtaat gtcattttac gcgctacaga aaaagacaaa gcgaatcgtt
    28381 acaaaacaat tcaagaaatg aaagatgatt tgagtagtgt tttacatgaa aatcgagcga
    28441 atgaagatgt ctatgaactc gataaaatga aaacgatagc ggtaccattg aaaaaagaag
    28501 atctagcaaa gcatattagt gaacataagt cgaatcaacc taaacgtgaa acgacgcaag
    28561 tacctattgt aaatgggcct gctcatcatc agcaattcca aaagccagaa ggtaccgtgt
    28621 acgaaccaaa acctaaaaag aaatcaacac gaaagattgt gctcttatca ctaatctttt
    28681 cgttgttaat gattgcactt gtttcttttg tggcaatggc aatgtttggt aataaatacg
    28741 aagagacacc tgatgtaatc gggaaatctg taaaagaagc agagcaaata ttcaataaaa
    28801 acaatctgaa attaggtaaa atttctagaa gttatagtga taaatatcct gaaaatgaaa
    28861 ttattaagac aactcctaat acaggtgaac gtgttgaacg tggtgacagt gttgatgttg
    28921 ttatatcaaa aggccctgaa aaggttaaaa tgccaaatgt tattggttta cctaaggagg
    28981 aagccttgca gaaattaaaa tcgttaggtc ttaaagatgt tacgattgaa aaagtatata
    29041 ataatcaagc accaaaagga tacattgcaa atcaaagtgt aaccgcaaat actgaaatcg
    29101 ctattcatga ttctaatatt aaactatatg aatctttagg cattaaacaa gtttatgtag
    29161 aagactttga acataaatcc tttagcaaag ctaaaaaagc cttagaagaa aaaggattta
    29221 aagttgaaag taaggaagag tatagtgacg atattgatga gggtgatgtg atttctcaat
    29281 ctcctaaagg gaaatcagta gatgaggggt caacgatttc atttgttgtt tctaaaggta
    29341 aaaaaagcga ctcatcagat gtcaaaacga caactgaatc ggtagatgta ccatacactg
    29401 gtaaaaatga taagtcacaa aaagttaaag tttatattaa agataaagat aatgacggtt
    29461 caacagaaaa aggtagtttc gacattacta gtgatcaacg tatagacatt cctttaagaa
    29521 ttgaaaaagg aaaaacagca agttatattg ttaaagtaga cggtaaaact gtagctgaaa
    29581 aagaagtcag ctatgatgat gtttaaatat aattgaagta aatgtaccga ggtttctatt
    29641 tggaagtctc ggtattttta tgttgagatt gcggtagttt taaaatgctt catgtcatca
    29701 tatacaggtg agcattgtct atgaattctg tatcactctc acaatattta tatcatttga
    29761 aatgatcatt tatatattaa ttttccagtc tttgatataa taaatgcagt aaaattaatc
    29821 gagaggtgcc attttgaaga caggtcgaat agtgaaatca attagtgggg tatatcaagt
    29881 agacgttaat ggcgaacgtt tcaatacaaa accacgagga ttatttagaa agaaaaaatt
    29941 ttcaccggta gttggagata tagtggaatt tgatgtacaa aacattaacg aaggctatat
    30001 tcatcaagtg tatgagcgga aaaatgagtt gaaaagacca cctgtaagta atatagatac
    30061 actagtaatt gtaatgagtg ctgtcgaacc aaatttttca acgcaattat tagatcgatt
    30121 tttagttatt gcacattcgt atcagttaaa tgcgagaatt ttggtgacaa aaaaagataa
    30181 aacaccaatt gaaaagcagt tcgaaattaa tgagttgttg aaaatatatg aaaatattgg
    30241 ctatgagact gaatttattg gaaatgatga tgatcgaaaa aaaattgtag aagcttggtc
    30301 agctggactt atagtactta gtggtcaatc aggtgtcggt aagtccactt tcttaaatca
    30361 ttatcgtcca gaacttaatc ttgagacaaa tgatatatca aaatcattaa atcgaggaaa
    30421 gcatactaca agacatgtcg aactattcga acgtcaaaac ggttatattg cagacacacc
    30481 tggattcagt gctttagatt ttgatcatat agataaagat gaaataaaag attattttct
    30541 tgaattaaat cgatatggtg aaacatgtaa gtttaggaat tgtaatcata tcaaagaacc
    30601 taattgtaat gttaagcatc aattagagat agggaatatt gcgcaattta gatacgacca
    30661 ttatttacaa ctatttaatg aaatttcaaa tagaaaggtt agatattaaa tgacaaaact
    30721 atatccatca ttattatctg ttgatttttt ggatttacaa catgaattaa aacgacttga
    30781 agaagcaggt gtcgacggag ttcattttga tgttatggat ggtcaatttg tgcctaatat
    30841 atctattggt ttaccaatat tagatgcagt aagaaaaggc acaacattac ctatagacgt
    30901 acatttgatg attgaaaatc cagaaaagta tattgcatca tttgcagaac atggtgccga
    30961 tatgatttca attcatgtcg aatcaacgcc tcatattcat cgtgctattc aaatgattaa
    31021 acatttagat aaaaaagctg gtgtagtaat taatcctggt acaccaatat cacaaattga
    31081 acctatttta gatattgttg attatgtact agtgatgaca gttaacccag ggtttggtgg
    31141 tcaatcattt attgatcaat gcgtagaaaa aatagcgggt cttaatgcta ttaaaatgga
    31201 acgtcaatta aactttgata ttgaagttga tggaggcgta aataccgata cagcgaaagt
    31261 atgtgttgaa aatggtgcta caatgctagt aacaggttca tttttcttta aacaagagga
    31321 ttataaaaaa gtcacacaag cattgaaagg ttgagtgtat atgcatataa atttattatg
    31381 ttctgatcga cacttgccgc aagatatttg ggccaaaagt aatgaaggta aatggggcgg
    31441 cgttgataga ggtgctttga ttttattgaa gcatcaaatt atcccttttt tctcagtggg
    31501 agactttgat tcagtcagta aagaagaacg ccaacttcta acagaacagt tacaaatcaa
    31561 accagttcaa gctgaaaaag ctgatacgga tttagcttta gcggttgata aagctgttgc
    31621 acttggattt gatagtatta caatttatgg tgcaacaggc ggacgattag atcacttttt
    31681 tggggcaatt cagttattat tgaaaaaagc atattataaa catgatgttc atatagaagt
    31741 tatcgatcaa caaaataaaa ttgaattatt gcctaaaggt caacatacag ttgaaaaaga
    31801 taagagttat ccgtacattt catttatacc gatgactgat gatgtagaac tttctctagc
    31861 aggttttaaa tataatttag ctagacaaat gcttaatata ggttctactt taactatttc
    31921 aaatgaaatt gagtctttgc aagcgaaagt aactgtacat gatgggttga ttttgcaaat
    31981 tagaagtaca gatttaaatt aagtatattc aaatatgcga agatttttaa taaagtccat
    32041 gagttattta ataaagtgac gtttatagta tagaatttat agcataataa tagatgaatc
    32101 acgctgtaat taatgaagat tatggcgtga tttattattg aaaggaaaat tgtaggtcct
    32161 agtacattga tttatgtgcc aaaatcgata ttgaacccat aagtccaatt tgacagagaa
    32221 ccattattaa acgttttaac caaattatat tgatagttcg aatttcacta tgaaccatta
    32281 tgtattgttt tgtaacaaaa aagaactaag aggttgagct cttagttctt aagtaaatgc
    32341 gtcctccaca taaggtcaca ttttttatgt gcaatatatt tattaaactc tagttacttt
    32401 accagatttt aaagcacgtg cagaaaccca aactttttta ggtttaccgt caactaggat
    32461 tctaactttt tgaaggttag cgttccatct acgtttagta gagtttaaag cgtgtgaacg
    32521 tctgttacca gtcgaagctt tacgacctgt tacgaaacat tgtttaccca tatgagtacc
    32581 tcctttaata aatataaat
//
LOCUS       DAKSQE010000019        32425 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.019, whole genome shotgun sequence.
ACCESSION   DAKSQE010000019 DAKSQE010000000
VERSION     DAKSQE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 32425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32425
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.019"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..250)
                     /locus_tag="O0V33_000739"
     CDS             complement(<1..250)
                     /locus_tag="O0V33_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCX8975405.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK"
     gene            596..2419
                     /gene="lepA"
                     /locus_tag="O0V33_000740"
     CDS             596..2419
                     /gene="lepA"
                     /locus_tag="O0V33_000740"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCX8975406.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCILRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
     gene            2926..4050
                     /locus_tag="O0V33_000741"
     CDS             2926..4050
                     /locus_tag="O0V33_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCX8975407.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRNLKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVNRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNAGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSRRFKKKFDQSIESVFG
                     QTINNLKEKKLIVEKNDAIALTNRGKVIGNEVFEAFLIND"
     gene            4151..5128
                     /gene="hrcA"
                     /locus_tag="O0V33_000742"
     CDS             4151..5128
                     /gene="hrcA"
                     /locus_tag="O0V33_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HCX8975408.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFSNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            5160..5786
                     /gene="grpE"
                     /locus_tag="O0V33_000743"
     CDS             5160..5786
                     /gene="grpE"
                     /locus_tag="O0V33_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCX8975409.1"
                     /translation="MTNKDESVEKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            5855..7687
                     /gene="dnaK"
                     /locus_tag="O0V33_000744"
     CDS             5855..7687
                     /gene="dnaK"
                     /locus_tag="O0V33_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCX8975410.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
     gene            7823..8962
                     /gene="dnaJ"
                     /locus_tag="O0V33_000745"
     CDS             7823..8962
                     /gene="dnaJ"
                     /locus_tag="O0V33_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCX8975411.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINDQPSNFKDRAKRFFKGE"
     gene            8966..9904
                     /gene="prmA"
                     /locus_tag="O0V33_000746"
     CDS             8966..9904
                     /gene="prmA"
                     /locus_tag="O0V33_000746"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCX8975412.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSYDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNELTYDDKLRQRIKDELLNLDELDQHNVQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASYLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIDDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            9906..10658
                     /locus_tag="O0V33_000747"
     CDS             9906..10658
                     /locus_tag="O0V33_000747"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCX8975413.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDKIAVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAHEEEAKDGELSNFKQTLQQFKAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            10665..12011
                     /gene="mtaB"
                     /locus_tag="O0V33_000748"
     CDS             10665..12011
                     /gene="mtaB"
                     /locus_tag="O0V33_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HCX8975414.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEEEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
     gene            12304..12480
                     /locus_tag="O0V33_000749"
     CDS             12304..12480
                     /locus_tag="O0V33_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCX8975415.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
     gene            12724..13407
                     /locus_tag="O0V33_000750"
     CDS             12724..13407
                     /locus_tag="O0V33_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCX8975416.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
     gene            13425..14414
                     /gene="floA"
                     /locus_tag="O0V33_000751"
     CDS             13425..14414
                     /gene="floA"
                     /locus_tag="O0V33_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HCX8975417.1"
                     /translation="MFSLSFIVIAVIIVVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            14431..15129
                     /locus_tag="O0V33_000752"
     CDS             14431..15129
                     /locus_tag="O0V33_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HCX8975418.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQQETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
     gene            15432..16379
                     /locus_tag="O0V33_000753"
     CDS             15432..16379
                     /locus_tag="O0V33_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCX8975419.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGTVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
     gene            16380..16847
                     /gene="ybeY"
                     /locus_tag="O0V33_000754"
     CDS             16380..16847
                     /gene="ybeY"
                     /locus_tag="O0V33_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCX8975420.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPDIDFSGLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRD"
     gene            16850..17194
                     /locus_tag="O0V33_000755"
     CDS             16850..17194
                     /locus_tag="O0V33_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCX8975421.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            17205..17609
                     /gene="cdd"
                     /locus_tag="O0V33_000756"
     CDS             17205..17609
                     /gene="cdd"
                     /locus_tag="O0V33_000756"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCX8975422.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSHFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            17610..18509
                     /gene="era"
                     /locus_tag="O0V33_000757"
     CDS             17610..18509
                     /gene="era"
                     /locus_tag="O0V33_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCX8975423.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            18537..19283
                     /gene="recO"
                     /locus_tag="O0V33_000758"
     CDS             18537..19283
                     /gene="recO"
                     /locus_tag="O0V33_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCX8975424.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNKWRGMGTLNSVDVISQHYKLQMDLYVSSYASLAAETIERSMDE
                     GDVAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKYDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(19434..20825)
                     /locus_tag="O0V33_000759"
     CDS             complement(19434..20825)
                     /locus_tag="O0V33_000759"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HCX8975425.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            21160..21783
                     /locus_tag="O0V33_000760"
     CDS             21160..21783
                     /locus_tag="O0V33_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCX8975426.1"
                     /translation="MELSQRQERIIEIVKTQGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHTVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            21794..22612
                     /locus_tag="O0V33_000761"
     CDS             21794..22612
                     /locus_tag="O0V33_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCX8975427.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDIDPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            22673..24472
                     /locus_tag="O0V33_000762"
     CDS             22673..24472
                     /locus_tag="O0V33_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCX8975428.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQS
                     NSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEILLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQHLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPE
                     PIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDF
                     YAENDQYNISDAVQYVNSNELRETLISLEQYSLNDEPYENEIDDYVNVINENGQETIE
                     SLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            24696..25802
                     /gene="rpoD"
                     /locus_tag="O0V33_000763"
     CDS             24696..25802
                     /gene="rpoD"
                     /locus_tag="O0V33_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCX8975429.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            25933..26610
                     /locus_tag="O0V33_000764"
     CDS             25933..26610
                     /locus_tag="O0V33_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCX8975430.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGI
                     AGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIHPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYST
                     QLIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHRQRLAQINDEI
                     AVIERVL"
     gene            26613..27713
                     /locus_tag="O0V33_000765"
     CDS             26613..27713
                     /locus_tag="O0V33_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCX8975431.1"
                     /translation="MKIADLMTLLDHHVPFRTAESWDNVGLLIGDEDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPHGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEYVDEVKIEFMIDACQKSWAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAANIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKTLLMNWFNTEKINIDVEASTINTDPFEYI"
     gene            27827..29173
                     /locus_tag="O0V33_000766"
     CDS             27827..29173
                     /locus_tag="O0V33_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCX8975432.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPEQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFFLIPTKGAAKVEKTLNLIDILNPYLCIVFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
     gene            29183..30073
                     /locus_tag="O0V33_000767"
     CDS             29183..30073
                     /locus_tag="O0V33_000767"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCX8975433.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQHFDPELKNKVMQQ"
     gene            30199..30984
                     /locus_tag="O0V33_000768"
     CDS             30199..30984
                     /locus_tag="O0V33_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCX8975434.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            31026..31889
                     /locus_tag="O0V33_000769"
     CDS             31026..31889
                     /locus_tag="O0V33_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCX8975435.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIPKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            31876..32286
                     /locus_tag="O0V33_000770"
     CDS             31876..32286
                     /locus_tag="O0V33_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCX8975436.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVM
                     DENYPGISFDTIYRNLHLFKDLGIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKV
                     IDYCPIDQIKLSLPGVNIHKHKLEVYGVCESCQD"
BASE COUNT        11937 a         4411 c         6108 g         9969 t
ORIGIN      
        1 atttatttgc agtcattaat tgtgatttaa tacggtcagc tttgtttgaa tgtattaaat
       61 tactttgagc agctttgtct actaacttaa ctgctaagct tactaattca tttttattat
      121 cagcgttatt tgaaacagct gttttagcgt ttttaactgc tgtacgcatt gcactctttt
      181 gtgaaatgtt gcgtgcttca gctttttcag ttgtttttac acgtttaatt gcagatttga
      241 tatttgccat ttctgtcacc tcctaaaagt gagttcatct tatcaaaatt ttatttgatt
      301 acaacaagaa atattttaac aaaagtctaa ccgttgtgca atcatttatt tgatggggtt
      361 agcttaaaat tttgcagatt taataaattg tgttggaaac acaatcaata cgttttataa
      421 ttataagtac gtaattaata tattaacata tcgcaagtat gtatttaaat aaagattgtt
      481 ataaattcaa agttcatcca agattatggc gtttgcattt acctattaaa aacgttatta
      541 tatcaaagat gcgaaagata atacgggttt attttatgaa agtgagaagg ataaaatgga
      601 taatgagcaa cgcttaaaaa gaagagagaa tataaggaat ttctcgatta tagcacatat
      661 tgaccacgga aaatctacat tggctgatag aattttagaa aataccaaat cagttgaaac
      721 aagagatatg caagatcagt tactagattc aatggattta gaaagagaac gtggtattac
      781 aatcaaatta aacgcagtac gtttaaagta cgaagctaaa gatggaaata cttatacatt
      841 ccatttaatc gatacgcctg gacacgtcga ttttacatat gaagtgtcac gttctttggc
      901 agcttgtgag ggcgcgattt tagtagtaga tgcggctcaa ggtatcgaag cacaaacatt
      961 agcaaatgtt tatttggcat tagataatga gttagagtta ttgcctgtta ttaacaaaat
     1021 tgatttacct gctgcagaac ctgaacgcgt gaaacaagaa attgaagata tgataggttt
     1081 agaccaagac gatgttgttt tagcaagtgc taaatctaac attggaattg aagagatact
     1141 tgagaaaata gttgaagttg tgccagctcc agatggtgac ccagaagcac cactaaaagc
     1201 gttaatattt gattctgagt atgatccata tagaggggta atttcatcga taagaattgt
     1261 agacggtgtt gttaaagccg gagataaaat tcgaatgatg gccactggta aagagttcga
     1321 agtaacagaa gttggaatta atacacctaa gcaacttcca gttgatgaat taacagttgg
     1381 tgatgttggt tacattattg caagtattaa aaatgttgat gattctaggg ttggtgacac
     1441 aatcacatta gctagtagac ctgcatcaga accattgcaa ggttataaga aaatgaatcc
     1501 aatggtatat tgcggactgt tcccaataga taacaaaaat tataatgatt taagagaagc
     1561 attagaaaaa ttacaattga atgatgcatc attagaattt gagcctgaat catcacaagc
     1621 attaggtttt ggttatagaa ctggtttctt aggtatgtta cacatggaaa taattcaaga
     1681 aagaattgaa agagaatttg gcattgaatt aattgcaacc gcaccatctg taatttatca
     1741 atgtatttta agggacggtt cagaagtgac ggttgataat ccagcacaaa tgccagaccg
     1801 tgataaaatt gataaaatat tcgagccata tgttcgtgca actatgatgg ttccaaatga
     1861 ctatgtaggt gcagtaatgg aattatgtca acgtaaacgt ggacaattta taaatatgga
     1921 ctatttagat gatattcgtg taaatattgt ttatgaatta cctttagctg aagttgtatt
     1981 tgatttcttc gatcaactta aatctaatac taaaggatat gcatcatttg attatgagtt
     2041 catcgaaaat aaagaaagta atttagtgaa gatggatatt ttattaaacg gtgataaagt
     2101 ggatgctcta agcttcatag tccatagaga ttttgcatat gaacgtggta aagcattagt
     2161 tgaaaaactt aaaacgttaa ttccaagaca gcaatttgaa gtacctgtac aagctgcaat
     2221 aggacaaaaa attgtagcac gtacaaatat taaatcaatg ggtaaaaacg ttttagctaa
     2281 atgttatggc ggtgacataa gccgtaaacg taaattactt gaaaaacaaa aagcaggtaa
     2341 agctaagatg aaagcagttg gtaatgttga aattccacag gatgcttttt tggctgtatt
     2401 gaaaatggat gacgaataat tttttaaaaa tcaattaaca atttacaatg aataaagttt
     2461 aataactaaa aagagggagc ctaggataaa ttaacgtcct gggctttaca atgttatatt
     2521 ggcagtcatc gacagagtta aaatgagctt ataacaatgg ggccccaaca tagaagctga
     2581 cgaaaagtca gcttacaata atgtgcaagt tggggtggga cgacgaaata aattttgcga
     2641 aaatatcatt tctgtcccac tcccttatgc atgagtttaa ctcatgtaat tctattttta
     2701 agtacatatt acctctggct aatgtgtaag agccactaca taataaatca ttagtggttc
     2761 tttattattt ctatctcact ccctcttaac atttataaat attaaaatga atcgatatat
     2821 tagacaatta ttgattaaac gttaaagtta aaagtaagaa taattgcaga tagtccaaca
     2881 ggatatagcc gattggataa acagtctgag aagcggggca ttaaaatgac agtacaaagt
     2941 gcatatatac atattccatt ttgtgtaaga atatgtacat attgtgattt caataaatat
     3001 tttatacaga accaacctgt agatgagtac ttagatgcac taataacaga aatgtctaca
     3061 gcaaaatata ggaacttaaa gactatgtat gtaggtggcg gcacaccaac ggccctttct
     3121 attaatcagt tggaaagatt acttaaagca atacgtgata cgtttacaat cacaggtgag
     3181 tatacatttg aagcaaatcc tgatgagtta actaaagaga aagtccaact attagagaaa
     3241 tatggagtaa acagaatttc aatgggcgtt caaacattca agccggagtt attgtctgtt
     3301 ttaggtagaa cgcacaatac tgaagatatt tacacgtcgg tgttaaatgc taaaaacgcc
     3361 ggtattaaat caatcagttt agatttaatg tatcatttac cgaaacagac gattgaagat
     3421 tttgaacaaa gtttagatct agctttagat atggatattc aacatatttc gagttacggc
     3481 ttaatacttg aacctaaaac ccaattttat aatatgtata gaaaaggttt gctcaaactt
     3541 cctaatgagg atttaggtgc tgacatgtat cagttgctga tgtctaagat agaacaatct
     3601 cctttccatc aatacgaaat atctaatttt gcattagatg gccatgaatc agaacataat
     3661 aaggtttact ggtttaatga ggaatattat ggatttggag caggtgcaag tggttatgta
     3721 gatggtgttc gttatacgaa tatcaatcca gtgaatcatt atatcaaagc tataaataaa
     3781 gaaagtaaag caattttagt atcaaataaa ccttctttga ctgagagaat ggaagaagaa
     3841 atgtttcttg ggttgcgttt aaatgaaggt gtgagtagta gaaggttcaa aaagaagttt
     3901 gaccaatcaa ttgaaagtgt ctttggtcaa acaataaata atttaaaaga gaagaaatta
     3961 attgtagaaa agaacgatgc gattgcactt acaaatcgag ggaaagtcat tggtaatgag
     4021 gtttttgaag ctttcctaat aaacgattaa aaaaaattga aatttcgagt ctttaacatt
     4081 gacttacttt gaccaatttg ataaattata attagcactt gagataagtg agtgctaatg
     4141 aggtgaaaac atgattacag ataggcaatt gagtatatta aacgcaattg ttgaggatta
     4201 tgttgatttt ggacaacccg ttggttctaa aacactaatt gagcgacata acttgaatgt
     4261 tagtcctgct acaattagaa atgagatgaa acagcttgaa gatttaaact atatcgaaaa
     4321 gacacatagt tcttcagggc gttcgccatc acaattaggt tttaggtatt atgtcaatcg
     4381 tttacttgaa caaacatctc atcaaaaaac aaataaatta agacgattaa atcaattgtt
     4441 agttgagaat caatatgatg tatcatcagc attgacatat tttgcagatg aattatcaaa
     4501 tatatctcaa tatacaactt tagttgttca tcctaatcat aaacaagata ttatcaataa
     4561 tgtacacttg attcgtgcta atcctaattt agttattatg gttatcgtat tttcatcagg
     4621 tcatgttgag catgtacatc ttgcttcaga cataccgttt agtaatgaca aactaaatac
     4681 aatatcaaat tttgttacta acaaactaac tgagtttaat cagaatttac aagatgatat
     4741 tgtctctttt gttcaatcag agcaggaaga aatatttatc aataaattgt taaatacgat
     4801 gaataatcat atttctaatc aaagtaatag catttatatg ggggggaagg ttaaacttat
     4861 tgatgcattg aatgaaagta atgtatcttc aattcagccc attctacaat atatagaatc
     4921 aaatagaatt gcagaattat tacaagatat atcttcacca aatattaatg taaaaattgg
     4981 taatgaaatt gatgacagct taagtgatat ttcaattgta acaagtcaat atcattttga
     5041 tgaaacgtta aaaggtcaaa tcgctgtgat tggacctaca gctatgcatt atcaaaatgt
     5101 cattcaatta ttaaatagaa tttggtaatg atggattaga aagtattgga ggacagaaaa
     5161 tgacaaataa agacgaatca gttgaaaaaa acactgaatc aacagttgaa gaaacaaacg
     5221 tcaaacaaaa tattgatgat tcagttgaac aagctgaaga aagtaaaggt catttacaag
     5281 atgaagcaat agaagaaacg tctgacgaga atgttattga agaaatagat ccaaaagatc
     5341 aaaaaattaa tgaacttcaa caattagcag atgaaaacga agagaaatat ttaaggctct
     5401 acgctgagtt tgaaaattat aagcgtagaa ttcaaaaaga aaatgaaata aataaaacat
     5461 atcaagcaca acgtgtgtta acagatattt taccagcaat agacaatata gaacgtgcac
     5521 ttcaaattga aggtgatgat gagactttta aatctcttca aaaaggtgta caaatggtgc
     5581 atgaaagttt gattaacgca ctaaaagata atggtcttga agttattaaa actgaaggtg
     5641 aagcatttga tccaaatatt caccaagctg tagttcaaga tgataaccct gattttgaat
     5701 ctggcgaaat cactcaagaa ctacaaaaag gatacaagct taaagataga gtattaagac
     5761 catcaatggt caaagtaaac caataactta aatttggcga aaagacattg tttaaaatta
     5821 aattaattta atgattaaat ggaggaattt tattatgagt aaaattattg gtatagactt
     5881 aggtacaaca aattcatgtg taacagtatt agaaggcgat gagccaaaag taattcaaaa
     5941 ccctgaaggt tcacgtacaa caccatctgt tgtagctttc aaaaatggag aaactcaagt
     6001 tggtgaagta gctaaacgtc aagctattac aaacccaaac actgttcaat ctattaaacg
     6061 tcatatgggg actgattata aagtagatat tgaaggtaaa tcatacacac cacaagaaat
     6121 ctcagctatg attttacaaa acttaaaaaa tacagctgaa agctatttag gtgagaaagt
     6181 tgacaaagct gtaattacag tacctgcata ctttaacgat gctgaacgtc aagcaactaa
     6241 agatgctggt aaaattgctg gtttagaagt tgagcgtatc attaatgaac caacagctgc
     6301 agcattagca tatggtttag acaaaactga taaagacgaa aaagttcttg tttttgactt
     6361 aggtggcggt acatttgacg tatctatcct agaattaggt gacggtgtat tcgaagtact
     6421 atcaacagcc ggtgacaaca aacttggcgg tgatgatttt gaccaagtaa ttattgacta
     6481 cctagttgca gaattcaaaa aagaaaatgg cgtagactta tctcaagata aaatggcatt
     6541 acaacgtttg aaagatgctg ctgaaaaagc taaaaaagac ttatcaggtg tatcacaaac
     6601 tcaaatctca ttaccattta tctcagctgg tgaaaacggt ccattacact tagaagtaaa
     6661 cttaactcgt tctaaatttg aggaattatc agattcatta attagaagaa caatggaacc
     6721 tacacgccaa gcaatgaaag acgctggctt aacaaactca gatatcgatg aagttatctt
     6781 agttggtgga tcaactcgta ttccagcggt acaagaagct gtcaaaaaag aaatcggtaa
     6841 agaacctaac aaaggtgtaa acccggacga agtagtggct atgggagctg caatccaagg
     6901 tggcgttatc acaggtgacg ttaaagacgt agtattatta gacgtaacac cactatcttt
     6961 aggtattgaa attttaggtg gacgtatgaa tacgttaatt gaacgtaaca ctacgattcc
     7021 tacatctaaa tctcaaatct attcaacagc agtagataat caaccatcag tagatgtaca
     7081 cgtattacaa ggtgaacgtc caatggctgc ggataataaa acacttggta gattccaatt
     7141 gactgatatt ccaccagctg aacgtggtaa acctcaaatt gaagtaacgt ttgatatcga
     7201 taaaaacggt attgtaaatg taactgcaaa agacttaggt acaaataaag aacaaagaat
     7261 tacaattcaa tcaagttctt cattatcaga cgaagaaatc gaccgtatgg taaaagatgc
     7321 tgaagttaac gctgaagcag ataaaaaacg tcgtgaagaa gtagacttaa gaaacgaagc
     7381 tgacagccta gtattccaag ttgaaaaaac tttaactgat ttaggcgaaa atatcggtga
     7441 agaagataaa aaatctgctg aagagaaaaa agacgctctt aaaactgctt tagaaggtca
     7501 agatatagaa gatattaaat ctaaaaaaga agaacttgaa aaagtgattc aagaattatc
     7561 agcaaaagta tatgagcaag cggctcaaca gcaacaacaa gcacaaggtg ctaatgctgg
     7621 tcaaaacaac gatagtactg tagaagatgc tgaatttaaa gaagtaaaag acgacgacaa
     7681 aaaataatta aacaatacaa ttagaatgtt gtcatgatct gaaaagtcaa agtcaataga
     7741 acattggctt tggcttttta tttggatagt atgacatatt acgttaaact atatgagtta
     7801 attaagaaaa ggagagatag ctgtggccaa aagagactat tatgaagttt taggcattag
     7861 taaagatgct tcaaaagatg aaatcaaaaa agcgtatcga aagctttcga aaaaatatca
     7921 tccagatatt aacaaagaag aaggtgcaga tgaaaagttt aaagaaattt ctgaagccta
     7981 tgaagtttta agtgatgata ataaacgcgc aagctatgat caatttggac atgacgggcc
     8041 acaaggtttt ggtggacaag gattcaatgg ctctgacttt ggcggtttta gtggcttcgg
     8101 tggcggcggt tttgaagata tctttagctc tttcttcggt ggcggcagac aaagagatcc
     8161 aaatgcaccg caaaaaggtg atgatcttca gtacacaatg acattgacat ttgaagaagc
     8221 ggtatttggt acaacaaaag aaatttcaat ccgtaaagat gtaacatgcg aaacatgtca
     8281 tggtgatggt gcaaagcctg gaacaagtaa aaagacttgt agttactgta atggcgctgg
     8341 tcatgtagct gtagaacaaa atacaatttt aggtagagtt cgcactgaac aagtttgtcc
     8401 taaatgtaat ggaagtggtc aagaatttga agaagcttgt ccaacatgtc acggaaaagg
     8461 tactgaaaat aaaacggtta aattagaagt gaaagtacct gaaggtgtcg acaatgaaca
     8521 acaaattaga ttagctggtg aaggctctcc aggcgtgaat ggaggacctg ctggtgattt
     8581 atacgtagta tttagagtta aaccatctga aacatttaaa cgtgatggtg atgatattta
     8641 ttacaaatta aatgttagct tcccacaagc agctttaggc gatgaaataa aaataccaac
     8701 attaaataat gaagttatgt taacaatccc agctggtaca caaacaggta agcaattccg
     8761 cttaaaagaa aagggtataa aaaatgttca tggctatgga tacggagatt tatatgttga
     8821 tattaaagta gtaacaccta ctaaattaac agatagacaa aaagaactaa tgaaagaatt
     8881 tgcccaatta aatggtgaag aaattaatga tcaaccttcg aattttaaag atagagcaaa
     8941 aagattcttt aagggagaat aaagtatgaa ctggacagag ctttcaatta ttattaatca
     9001 tgaagcagta gaattggcta ccaatatact tgaaaatcat ggatcaaatg gtgtcgtgat
     9061 agaagattca tatgatttaa ttaatcaacc agaagataaa tacggtgaaa tttacgcttt
     9121 gaaaaaagag gattatccag ataagggagt aagattgaaa gcctatttta atgagttgac
     9181 ttatgatgat aagttgcgac agcgaattaa agatgagtta ttaaacttag atgaacttga
     9241 tcaacataac gttcaattca gtgagcaaat cattgcagaa acggattggg aaaatgaatg
     9301 gaaaaactat ttccatccat tccgagcgtc gaagaagttc acaatagtcc ctagttggga
     9361 aacatatgct aaagaagcgg atgaagagct ttgcattgag ctcgatccag gtatggcttt
     9421 tggaacagga gatcatccga ctacaagtat gtgtttgaag gcaatagaaa catatgtatt
     9481 gccacagcat tcagtaattg atgttggtac tggctcaggt atattaagta ttgcaagtta
     9541 tctaatcggt gtaaaacgta ttaaagcgtt ggatattgat gaaatggcag tgagtgtagc
     9601 taaagaaaac ttcagaagaa atcattgtga aacgttaatt gaagctgttc caggtaactt
     9661 attgaaagac gaaacagaaa aatttgatat tgtaatagca aatattttag cgcatattat
     9721 tgatgaaatg attgatgatg cttataatac tctaaatgaa ggcggttatt ttattacttc
     9781 tggtattata aaagagaagt atgaaggtat acagtcacat atggagcgtg taggttttaa
     9841 aattatttca gaacaacatg acaacggttg ggtttgtctt gttggccaga aagtgagtga
     9901 ataatgtgca acgttatttc atagaccaaa acgctgatgt aagtcagcgt ttttttatta
     9961 caaaaaaaga agatattcat catataacaa atgtaatgag atatgatgtt ggtagtaaaa
    10021 ttattttaac ttttaatgat caaacagttt atttatgtga aattgctgat ataacaagtg
    10081 ataaaattgc agttgattta aaagaaaaac aaaatattaa tacagaattg ccagttgatg
    10141 ttacgatttg cagtggacta attaaagctg acaaatatga gtggttgcta caaaaagcta
    10201 ctgaattggg tgcttcatcg tttatagctg ttagcatgga acgttcaatt gttaaattaa
    10261 acgaggcaaa agttgccaaa aagatagaac gttggcaaaa aataattaaa gaagctgctg
    10321 aacaaagcta tcgtttagtg attccatcta ttcaattcga gtcgaattta aaattaattt
    10381 gtgatactat tgataattat gactatattc ttattgcaca tgaagaggaa gcaaaagatg
    10441 gtgagttaag caatttcaag caaactttac aacaattcaa ggctcaggat aaagtgttga
    10501 tgatttttgg tcctgagggt ggtttgtcag aaaatgaaat ttcattattt agtaatacta
    10561 gtacagttgt tggtcttgga ccgagaattt tacgggcaga aactgcgcca ttgtatgcgt
    10621 taagtgcaat aagttatgaa aaagaattaa tggggtgaat atttatgtca acagttgcgt
    10681 ttcacacatt aggttgtaaa gtaaaccatt atgaaactga agcaatctgg caattattta
    10741 aagaagcaaa ctatgagcgc gttgactttg aagcgaatgc tgatgtattt gttattaata
    10801 cttgtacagt aacgaataca ggtgataaaa aaagtcgtca aattattaga cgtgcaataa
    10861 gacaaaatcc tgatgctgta atctgtgtaa caggttgtta tgcgcaaact tcatcagctg
    10921 aaattatgga aattcctggt gtcgatgtag tagttggtac acaagataga cataaactat
    10981 taggttacat tgatgaattc cgtaaagaac gccaaccaat taatggtgtt ggaaatatca
    11041 tgaaaaatcg taaatatgaa gaattagatg tcccttattt tacagataga acacgtgcgt
    11101 cattaaaaat tcaagaaggt tgtaacaact tttgcacatt ctgtattatt ccatgggctc
    11161 gtggcttaat gcgttcaaga gatccggaaa aagtagttga acaagcgacg caactagtga
    11221 attcaggata taaggaaatt gtattgacgg gaattcatac aggtggatat ggtcaagatt
    11281 taaaagatta taacttggcc caattattac gtgatcttga aacgattaat ggattagaac
    11341 gaattcgaat ttcttcaatt gaagcaagtc aacttacaga tgaagtgatt gacgttttag
    11401 aacgttcaac aaaagttgtg cgtcatttgc atattccgtt acaatctggt tcagatacag
    11461 tattaaaacg tatgagacgt aagtatacaa tggataggtt ttcggaacga ttaacaaaat
    11521 tgcataaagc tttaccagac ttggcagtta cgagtgatgt aattgttggt ttcccaggtg
    11581 aaactgaaga agagttccaa gaaacatatg attttatcgt aaagcataag ttctctgaac
    11641 tgcatgtttt cccttattct cctagaattg gcacgccagc tgcaagaatg gatgaccaaa
    11701 ttgatgaaga aattaaaaat gaacgcgtgc ataagttaat tacgcttagc aatcaacttg
    11761 gaaagttata tgcgtctaaa tttgatcaag atgtgcttga agtaattcct gaggagcagg
    11821 gtgacacaga aggtacatta gttggatatg cagataatta tatgaaagta caatttgaag
    11881 gtgacgaatc actcataggt caaattgtaa aagttaaaat tacgcaagca aattatccat
    11941 taaatgaagg gcaagcaatt aaagttgttg atttcgcaac aaataaatct gatagagaag
    12001 ttttagttta agtattcctt gattcatacc ataaggttcc gtattaaaga tataggtttt
    12061 gtaacgttgt tttaacagct tcacaattta ctatataaac aactcttctc attaaataaa
    12121 ttattaagag atttttagaa caaaatctaa aagtctttgt gattttgatg tgaaatatgt
    12181 ttcaaataaa aaacaattta ctaattgacc ataaattaca gatatattat acttataaat
    12241 gcatagtttt actgtgcaat tgactataaa gttccgttga tatttggagg gagggaaata
    12301 cagatgtcta aaacagtagt acgtaaaaat gaatcacttg aagatgcgtt acgtagattt
    12361 aaacgttcag tttctaaaag tggaacaatc caagaagtac gtaaacgtga attttacgaa
    12421 aaaccaagcg taaaacgtaa aaagaaatca gaagctgcac gtaaacgtaa attcaaataa
    12481 ttaatacctc tgttgactcc ctcaacacga atattaatta tataaaataa acatcacaag
    12541 ttagtgtctg acactaatat gtgatgtttt tttgttgtca atttttaatt taaaaaaatt
    12601 atatagttta taaataatca aattgatatt ctataggttc ttataactat aaagtatatt
    12661 caatttcatg tataattaat gtgagggcga ggtgaaattg tgagttataa taatttttta
    12721 caaatgacaa ctatcttgga atcaacggct ggagatactt gggttgaaca agttagtaat
    12781 ataattgttc aacctatttt tacgttaata ttaacctgtt tgacattctt aggatttgta
    12841 tatcaacttt actctaaaaa aatcaatgca gctggtatta tcgccacatt atcattactt
    12901 attttatttt tgggatttct aatccaagga aatgtcaata tgcattctat cttaatattc
    12961 tcaattggcg ttatattagt tgtaattgaa ttatttgtag ttggtgcagt aattggtatt
    13021 attggcatga tactgataac tataagcata acaacgctcg gtgataattt gctatttatg
    13081 cttgcgaatg ttatcgttgc cttgatttta acgattgtag aatgggtgat attagtgaag
    13141 attttcaaca gaaagattcc gtttttggat aaagttatct taaaagattc aactaattct
    13201 gagtcaggtt acaattctca tgataaccgc tcgcacctcg taggaaagac tgctcaaaca
    13261 gttacagatc ttcgacctgc agggattatt ttttgtgaaa atgaacgtat tgatgctgtt
    13321 tcagatggca actttatttt acgcaataaa acggtaaaaa tccttgaagt tgaaggaaca
    13381 agagtagttg tgagggaagt agattaatta aaaggagcga taccatgttt agtttaagtt
    13441 ttatcgtaat agcagttatt atagtagttg cattacttat tttattctca tttgtaccga
    13501 ttggtttatg gatttcagcg ttagcagctg gcgttcatgt tggtataggt acattggttg
    13561 gtatgcgttt acgtcgtgta tctccaagaa aagttatagc gccattaatt aaagcgcata
    13621 aagcaggact agcattaaca acaaaccaat tagaatcgca ttatctagca ggaggaaatg
    13681 ttgacagagt tgttgacgct aatattgctg cacaacgtgc tgacattgac cttcctttcg
    13741 aacgtgcagc tgctattgac ctagcaggac gtgacgtatt agaagcggtt caaatgtctg
    13801 ttaatcctaa agtcattgaa actccattta tcgcaggtgt agcaatgaat ggtattgaag
    13861 tgaaagccaa agctcgtatc acagttagag ctaatattgc tcgacttgtt ggtggtgctg
    13921 gtgaagaaac aatcatcgca cgtgttggtg aaggtatcgt ttcaacaatt ggttctagta
    13981 agcatcatac agaagtactt gaaaacccag ataatatttc taaaacagta ttaagcaaag
    14041 gtttagattc aggtactgca tttgaaattt tatcaattga tattgctgac gttgatatta
    14101 gtaaaaatat tggtgcagac ttacaaactg aacaagcatt agcagacaaa aatattgcac
    14161 aagcaaaagc tgaagaacgt agagctatgg ctgtagcaac tgagcaagaa atgaaagcgc
    14221 gtgtacaaga aatgcatgct aaagtagttg aagccgaatc tgaagtacca ttagctatgg
    14281 ctgaagcatt acgttcaggt aatatcagtg ttaaagatta ttataatttg aaaaatatcg
    14341 aagctgatac aggcatgaga aatgcaatta ataaacgaac tgatcaaagt gatgatgagt
    14401 cacctgaaca ttaagtcgag aggtgattaa atgagtgtcg gtattctaat ttttgtcata
    14461 tcagtgatca tttctatcat tactactatg cgcgaaaata gtcataaaga tagacaaaat
    14521 caaaagccac ctcaaaaaac atctaccgat aatgaaccaa aaaaaggtgg cttttttgaa
    14581 gaaattgagc gaacgtttaa agaaataagt gaagaattaa atgaagaaga aaagaaatca
    14641 tcgaaacgaa aatacgatga tacgttacca cctttattcg atgaacttcc aaaggaagag
    14701 cctaaatcga aacctgttgt agaacctatg gcacctaaaa aacaacaaga aacaaaaccg
    14761 atgacagaga aaccaattac agtgcctaaa gcagaaccgg tggagcagaa acatagacct
    14821 tctagacaag ataattctga cgaaattaga cgtcaattag aaaaatcact tagagatgat
    14881 attaaaacga ttcgtactga cattgataga gaaaaagaaa agcaaattgc taaaatggaa
    14941 aaacgtgcta gagatattat tgaggataaa tacttatctg aacgtacaaa acgtttgaaa
    15001 ttaaagcagt tgcttaattc tcaaaatgtc gaaaaagatt tgactaaatc agcgttccaa
    15061 tttgataaag atgaagtaat caatggtatg atatggtcag aaattttagc taaaccaaaa
    15121 caattataaa atttttgaaa acaagcacta tcgtaatggt agttgcttgt ttttttacgt
    15181 taagaaaaat tgaaaatcaa agagaatctt tcgagaaata tgagttgttt aaattacaac
    15241 aaaaaattga ttagtctaaa attgaatctg tttttatgac aaggtgaaaa gtataaatga
    15301 ttattttaaa ttaaagaaaa atcagtaagt caatgcaaag atgtttaaat caatcaattg
    15361 catgatataa ttaagtagat attaaagcat catagaatga atataaatga tatatgaaaa
    15421 ggagcgcgtg tatgcctgga attatacaaa tagacgatat gaaccaatct caagctttaa
    15481 ttggaaataa tgatgaacat ttaaaagcaa ttgaagagag tttcgatgtt gtcatccatg
    15541 caagaggaca agaagttgcc gttaaaggta caaaaataga aaacgtagaa aaagcggaat
    15601 cagtattaat caatttgctg aaggttattg atttaggtaa taatattaca attaaagatg
    15661 ttgaagcagc tattaaaatg gcgcataata acacaattca acatctgtta gatttatatg
    15721 atgaagagat aactaaagat gcatttggta agacgattcg tgcgaaaacg atggggcaac
    15781 gtatatatgt taatgccatg aaaaataatg atttagtatt tggtataggt cctgctggta
    15841 caggtaagac attcttagct gtagtttatg cagcaaagca actccgtaaa ggtactgtta
    15901 aacgtattgt attaacaaga cctgctgttg aagcaggaga gtcacttgga tttttaccag
    15961 gagatttgaa agaaaaggta gatccatatt taagaccttt atatgatggt ctatatactg
    16021 ttcttgggcg tgaacaaaca gagcgattta ttgaaagagg cattatcgaa atagcgccac
    16081 ttgcatatat gcgcggacga acattagaag atgcatttgt aattcttgat gaggcgcaga
    16141 atacgacaca tgcgcaaatg aaaatgtttt taacaagact aggttttggc tcaaaaatgg
    16201 tagttactgg tgaccaaact caaatcgatt tacctaaagg tgttaaaagt ggacttaagg
    16261 aagcggtcag taggttacac aacgttaaag gtataagtat attgaaatta gatcagagcg
    16321 atgtagtaag acatccattg gtaagtaaaa tcattgaaca ttatgaagga gagaattaaa
    16381 tgtttacgat agattttagc gatcacacag gcttagttaa agatgcttgg tataaacaaa
    16441 ttgaagattt attagaattt gctaaaaaag aagagcatat agaagacgat gctgagcttt
    16501 ctgttacatt tgtagataaa caagaaatac aagaaattaa tcgaacatat agagataaag
    16561 ataaagttac agatgtaatc tcatttgctt tagaagagga tgagccagat attgatttta
    16621 gtggtcttga tataccacgt gttttagggg atataattat ctgtacggat gtagcgcaag
    16681 aacaagcaaa caattacgga cattcttttg aacgagaatt aggattttta gcattacatg
    16741 gatttttgca tctattaggt tatgatcata tgactgaagc ggatgaaaag gaaatgtttg
    16801 gtcgacaaga tacaatatta aacgcatatg gattaacacg agactaatta tgaaaaggtt
    16861 taaatatgca cttgatgggc tgaaaatctt aattcaaaaa gactataaat ttcttttaca
    16921 tgtgtttgca atgattgttg ctattgtctt tggtctcgta ctaaatatta atcggattga
    16981 gtggatattt atactcattg ctattgcatt agttctcact gttgaagctt taaacactgc
    17041 tattgaatat gttgtagatt tagtgaccgt tgaatatcat gatttagcta aatacgctaa
    17101 agatatagcg gcttttagtg tacttatagt ttcaatatta gcatttatta taggtttaat
    17161 agtattttta ccacatttta tagcgttatt ttagggaggc atatatgagt tatcaacctc
    17221 attattttca agaagttaga aaagcacaac aagaatcata ttcgccatac agtcatttta
    17281 aagtaggggc ttatttaaaa acgaaagacg gtagaacttt ttatggtacc aatgtagaaa
    17341 atgcttctta tccattatcg atatgtgctg aacgagctag tttggtatcg gcaatttctc
    17401 aaggatacag accaggtgat tttgaatcaa taactgtaac cgtagatgca gataaaccgt
    17461 catcaccttg tggtgcatgt cgtcaagttt tgaaggaatt atgtgatgat gatatgcctg
    17521 tgtatatgac aaatcataaa ggagatatgg ttatgatgac agtcgcagag ttactaccat
    17581 ttggattttc aggaaaggat ttagaataaa tgacagaaca taaatcagga tttgtttcaa
    17641 ttataggtag accaaatgta gggaagtcaa catttgttaa tagagtgatt ggccataaaa
    17701 tagcaatcat gtccgataaa gctcaaacaa ctagaaataa aattcaaggt gtaatgacaa
    17761 gagatgatgc gcaaattata ttcattgata cgccaggtat tcataaacct aaacacaaat
    17821 taggtgacta tatgatgaaa gtcgctaaaa atacattatc tgagatagat gcaatcatgt
    17881 ttatggttaa tgccaatgag gaaattggac gaggcgatga atatattata gaaatgttga
    17941 aaaatgttaa gacaccagta tttttagtat taaataaaat agatttagtg catccagatg
    18001 aattaatgcc aaagattgaa gaatatcaaa gttatatgga ctttacagag attgtaccta
    18061 tttcagcatt agaagggcta aatgtagatc attttattga tgtattaaag acgtatttac
    18121 ccgaaggacc aaaatattat ccagatgatc aaatttcaga ccatcctgaa caatttgtag
    18181 tgggtgaaat cattcgtgaa aaaatccttc atcttacaag tgaagaaatc cctcatgcga
    18241 ttggtgttaa tgtggaccgt atggttaaag aaagcgaaga tcgtgttcat atcgaagcaa
    18301 ctatatatgt tgaaagagat tcgcaaaaag gaattgtcat tggaaaaggc ggtaaaaagt
    18361 taaaagaagt aggaaaacgt gcgagacgtg atatagaaat gcttctaggc tctaaagttt
    18421 acttggaatt atgggtcaaa gttcaaagag actggcgaaa caaagttaac tttattcgcc
    18481 aaattggtta tgttgaagac caagattaat cttaaaagtg gtgaagataa ttgttaatgc
    18541 gccaaaaagg gattatcatc aaagcagttg attatggtga atctgataaa attatcacga
    18601 ttttaaatga gcatggtgca aaagtaccac ttatggcaag gcgtgctaaa aaagttaaga
    18661 cgggtttgca agcgcaaacg caattgtttg tttatggttt gtttatttac aataagtggc
    18721 gaggtatggg aacgttaaat tctgtagatg ttattagtca acattataaa ttacaaatgg
    18781 acctttacgt aagcagttat gcgtctctgg cagctgaaac tattgagcgt tcaatggatg
    18841 aaggggacgt tgcaccatat aactatcaat tattacaatt tgttcttgaa aaaatagaat
    18901 caggtacatc tgcacagtta atgtcagtcg ttgttatgtt aaagtgtatg aaacgatttg
    18961 gttttaccgc atcatttaat cgctgtgctg tgagtggtaa tgacacacaa gcagatttaa
    19021 taggttatag ttttaagtat gacggtgcta tttcaaggca agaggcttct aaagatgtac
    19081 atgcagttat attatcgaat aaaacactat atttattaga tgtattacaa aaattaccaa
    19141 tagataaaat gaattcattg aatatccatc aagaaattat tgatgaaatg tcagatatca
    19201 ttttaatgtt atatcgtgaa tatgcaggta tgttttttaa aagtcagaaa ctaatcaacc
    19261 aattaaaaag attggaacaa taacataaaa taataaaagg tattcaagta gccacataga
    19321 tgtgtttatt tgaatacctt tttcgaatag aaaagagata ctggcaattt tactaaccag
    19381 tatctctttt tataaattat atagccacca catatggtgg agagtctttt taattagaat
    19441 tttgtttttt cagttaagaa agcttctaac tctgagattg gcatacgaac ttgttccatt
    19501 gaatctctgt cacgtactgt aacttgatta tcttctaatg aatcaaagtc gaatgttaca
    19561 caataaggtg taccaatttc atcttgacga cgatatcttt taccgataga ttgtgattca
    19621 tcgaaatcga ttgagaattt agaacttaat tgctcaaaaa tcttaatcgc ttcgccagat
    19681 aatttcttgc ttaaaggtaa aatcgctgct ttatatggtg ctaatgcagg atggaagtgt
    19741 aaaactgtac gtgcatcttt actaccttca acgccttctt catcatatgc atcacataag
    19801 aaagctaatg ttacacgatc tgcaccaagt gatggctcga tacaatatgg aatatatttt
    19861 tcgttcgttt ctggatcgtg gtatctgaaa tcttcaccag agtgttcagc atgtttacgt
    19921 aagtcgaagt ctgtacgact tgcgataccc cataactcac cccaaccaaa tgggaattta
    19981 tattcaatat cagttgttgc atttgagtaa tgagataatt catcttcatc atgatcacgt
    20041 aaacgcatat tttcactact catatttaag cttgttaacc agtcacttgc aaaagttttc
    20101 caataatttt gccattcgat ttcttcacca ggtttacaga agaattcaag ttccatttgt
    20161 tcaaattctc ttgttctgaa aatgaagtta cctggagtga tttcattacg gaatgattta
    20221 ccaatttgac cgataccaaa tggtaatttt ttacgcattg aacgttgcac gtttttatag
    20281 tttacaaaaa taccttgtgc tgtttcagga cgtaagaaaa tttcatttgt agaatcttca
    20341 gttacacctt ggaatgtttt aaacattaaa ttgaattggc gaatttcagt ccagttagca
    20401 gttttactta caggacaaac aataccttca tcgtcaataa tttttttcat ttgttcaaaa
    20461 cttaaaccat cggcaatgaa gttttcatca cctttaacat cttgcatata atcttcaatt
    20521 aatttatcag cgcgatatcg aattttacta tctttattat caatcattgg gtcgttgaag
    20581 ttgtttaagt gtcctgaagc ttcccatact tttggattca ttaagattgc agcatcgata
    20641 ccaacgttaa acggtgattg tgtaatgaat ttttgccacc aagctttttt aacattattc
    20701 tttaattcaa cacctaaagg accataatcc catgtgtttg ataaaccacc gtaaatatca
    20761 ctaccaggga acacaaaacc tctgtgtttt gctaatgaaa caattgtatc catatctttt
    20821 gccataatta cctctcctca tacatgaaaa cgccccatgg aaaataacag catagtaaat
    20881 atgctttact tccatgggac gagttaatat tttaaattgt atataataca aaataagtta
    20941 cgtatttaac ccgcggttcc acccaaatta gtgtagtcac tcgcttttat tttaaaatga
    21001 ttcgttgcgc caatcttatt gttaagctta cactatcctt aactcgctgt acgtttattc
    21061 taattgatag attatttaaa ggcaagtaaa attaatgtgt ttgtgacatg aaagaaatat
    21121 cggtataata ataagtgaat aaaaaagggg tgaagaccta tagaactcag tcaaagacaa
    21181 gaacgaatca tcgaaattgt taaaactcaa ggacccatta ctggtgaaca aatagcagat
    21241 aagttgaatt taacaagagc aacgcttaga ccagatttag cgatattaac aatgtcaggt
    21301 tttttagaag cgcgtccccg agttggatat tattattcag gtaaatcaaa aggtaaattt
    21361 tttaatgaaa aacttcgaca atttgaggtt aaggactata tgtctcaacc tgttgtgcta
    21421 agagagaata caacagttta tgacgctatt tgtacaattt ttttagaaga tgtaagtaca
    21481 ttatttatta ttaatgaaga taacgatttt gttggtgtgt gttcaagaaa agatttatta
    21541 agagcttcaa tgattggagc agatattcat acagtaccta tcagtgttaa tatgacacgt
    21601 atgcctaatg tcacttattt agaggaaagc gaattagtca tatacgcagc agatagaatg
    21661 attgaaaaag aaattgattc gattccaatt gtaagaaaaa aagataatca aaagtatgaa
    21721 gtaattggaa gaatttccaa aacaacaata gctaagttat tagtagcatt atataaagaa
    21781 taggtgagaa gtaatggaaa aaattaaaat tatcgtagct tcagattcta taggcgaaac
    21841 ggcagagtta gttgctaggg caggtatttc acaatttaat cctaagcaat gtaaaaatga
    21901 attattaaga tatccatata ttgaatcttt tgaagatgtt gatgaagtga ttcaagttgc
    21961 taaagataca aatgctatca ttgtttatac acttattaaa cctgaaatga agcaatatat
    22021 gagtgagaaa gtagcagaat tccaattgaa gtccgtcgat attatggggc cattaatgga
    22081 tttattatca gcatcagttg aagaaaaacc ttataatgag ccaggtatcg ttcatagatt
    22141 agatgatgca tatttcaaga aaattgatgc gatagagttt gcagttaaat atgatgatgg
    22201 taaagatcct aaaggattac ctaaagctga tattgtttta cttggtattt cgagaacttc
    22261 aaagacacca ttatcccagt atttagcgca taaaagttac aaagttatga atgtaccgat
    22321 tgtaccagaa gtgacaccgc cagatggctt atatgatatt gatccaaaga aatgtatcgc
    22381 acttaaaata agtgaagaaa aattaaatcg cattagaaaa gagcgactaa aacaattagg
    22441 actaggtgac acagctcgat atgcaacaga agcacgaatt caagaagaat tgaattactt
    22501 tgaagaaatc gtaagtgaaa ttggatgtcc tgtcattgat gtttctcaaa aagcaatcga
    22561 agaaacagca aacgatataa tccattatat tgaacaaaat aaatcgaaat gatttcattt
    22621 ttgtcaaaaa ttaggtataa tagtataact aatgcttaat aggtgattta atttgcgaat
    22681 agatcaatcg atcattaatg aaataaaaga taaaaccgat attttagact tggtaagtga
    22741 atatgtaaaa ttagaaaaga gaggacgcaa ttatataggt ttgtgtcctt ttcatgatga
    22801 aaagacacct tcatttacag tttctgaaga taaacaaatt tgccattgtt ttggttgtaa
    22861 aaaaggtggc aatgtttttc aatttactca agaaattaaa gacatatcat ttgttgaagc
    22921 ggttaaagaa ttaggtgata gagttaatgt tgctgtagat attgaggcaa cacaatctaa
    22981 ctcaaatgtt caaattgctt ctgatgattt acaaatgatt gaaatgcatg agttaataca
    23041 agaattttac tattacgctt taacaaagac agtcgaaggc gaacaagcat taacgtactt
    23101 acaagaacgt ggttttacag atgcgcttat taaagagcga ggcattggct ttgcacccga
    23161 tagctcacat ttttgtcatg attttcttca aaaaaagggt tacgatattg aattagcata
    23221 tgaagccgga ttattatcac gtaacgaaga aaacttcagt tattacgata gatttcgaaa
    23281 tcgtattatg tttcctttga aaaatgcgca aggaagaatt gttggatatt caggtcgaac
    23341 atataccggt caagaaccaa aatacctaaa tagtcctgaa acacctatct ttcaaaaaag
    23401 aaagttgtta tataacttag ataaagcacg taaatcaatt agaaaattag atgaaatttt
    23461 attactagaa ggttttatgg atgttataaa atctgatact gctggcttga aaaacgttgt
    23521 tgcaacaatg ggtacacagt tgtcagatga acatattacc tttatacgaa agttaacatc
    23581 aaatataaca ttaatgtttg atggggattt tgcgggtagt gaagcaacac ttaaaacagg
    23641 tcaacatttg ttacagcaag ggctaaatgt atttgttata caattgccat ctggcatgga
    23701 tcctgatgaa tacattggta agtatggcaa cgacgcattt actgcttttg taaaaaatga
    23761 caaaaagtca tttgcacatt ataaagtgag tatattaaaa gatgaaattg cacataatga
    23821 cctttcatat gaacgttatt tgaaagaact gagtcatgac atttcactta tgaagtcatc
    23881 gattttgcaa caaaaggctt taaatgatgt tgcaccattt ttcaatgtta gtcctgagca
    23941 attagcaaac gaaatacaat tcaatcaagc accagccaat tattatccag aagatgagta
    24001 tggcggttac attgaacctg agccaattgg tatggcacaa tttgacaatt tgagccgtca
    24061 agaaaaagcg gagcgagcat ttttaaaaca tttaatgaga gataaagata catttttaaa
    24121 ttattatgaa agtgttgata aggataactt cacaaatcag cattttaaat atgtattcga
    24181 agtcttacat gatttttatg cggaaaatga tcaatataat atcagtgatg ctgtgcagta
    24241 tgttaattca aatgagttga gagaaacact aattagctta gaacaatata gtttgaatga
    24301 tgaaccatat gaaaatgaaa ttgatgatta tgtcaatgta attaatgaaa acggacaaga
    24361 aacaattgag tcattgaacc ataaattaag ggaagctaca aggattggcg atgtagaatt
    24421 acagaaatac tatttacagc aaattgttgc taagaataaa gaacgcatgt agcatgtggt
    24481 tttaaagaat aatacgaata atgattatgt caaaatgtat aagggtaaat gatagttacc
    24541 gcatttaaac aacactattg aaaaataaat attgggatta gttcctattt gtaaaataaa
    24601 attaaaaata tggatgaatt aattaagaat ttagtttaaa atagcaatat tgaataaatt
    24661 tcgaatgttc atatttaaaa tcgggaggcc gtttcatgtc tgataacaca gttaaaatta
    24721 aaaaacaaac aattgatccg acattaacat tagaagatgt taagaagcaa ttaattgaaa
    24781 aaggtaaaaa agagggtcat ttaagtcatg aagaaattgc tgaaaaactt cagaatttcg
    24841 atatcgactc tgatcaaatg gatgatttct ttgatcaatt aaatgataat gatatttcac
    24901 tagttaatga aaaagatagt tcagatactg acgagaaact gaatccaagt gatcttagtg
    24961 cccctccagg tgttaaaata aatgacccag ttcgtatgta ccttaaagaa attgggcgtg
    25021 ttaacttatt aagtgcacaa gaagaaatcg aattagccaa acgtattgaa caaggtgatg
    25081 aagtagcaaa atcaagactt gcagaagcga acttacgttt agttgtaagt attgctaaaa
    25141 gatacgtagg tcgtggtatg ttattccttg atttaatcca agaaggtaat atgggtctta
    25201 ttaaagctgt tgaaaaattt gactttaaca aaggatttaa gttttcaaca tatgcaacat
    25261 ggtggattag acaagcaatc actcgtgcaa ttgctgacca agcacgtaca attcgtatcc
    25321 ctgtgcatat ggtagaaaca attaataaat taattcgtgt tcaacgtcaa ttattacagg
    25381 acttaggtcg agatccagca ccagaagaaa ttggtgaaga aatggattta ccagcagaaa
    25441 aagttcgtga aattttaaaa attgcgcaag aacctgtttc attagaaaca ccaattggtg
    25501 aagaagatga tagtcattta ggggacttta ttgaggatca ggaagcacaa agtccttcag
    25561 atcatgctgc ttatgaatta ttaaaagagc aattagaaga tgtgcttgat acattaactg
    25621 atagagaaga aaatgtatta cgattaagat ttggtcttga tgacggcaga acaagaacac
    25681 ttgaagaagt tggtaaagtt ttcggtgtta cacgtgaacg tattcgacaa atcgaagcaa
    25741 aagcacttag aaaattaaga catccaagtc gtagtaaacg tttgaaagac tttatggatt
    25801 aatttatatc aattttaatg attgatacca agacatgaat aaacgcttat ttatttttag
    25861 attaagattt aacttgagtg aggttggtac attgaataat gttcaacctc ttttttcata
    25921 aaggagaata aaatgatttc gttaaataac cgattaacga cggtaagtcg atttttaaaa
    25981 caaggtacaa tcgcggatat tggctcagat catgcttatt tgccaatcta tgcaatacaa
    26041 aaccatttat gcgaatgcgg tattgctgga gaagttattc aaggcccttt tcaagctgct
    26101 gtgaaaaatg tagctgcaaa ccaattagta gatagaattg atgtacgttt aggggatggc
    26161 ttaagtgtta ttcatcctga ggatgtaatc gataatatca caatttgtgg tatgggcgga
    26221 ccacttatcg ctaaaatttt aaaagatggg caagataagt taagtcaaca tccaaggttg
    26281 attttacaaa gtaatatcca aactgaaaat ttaagacaaa cattacaaca attgaattat
    26341 gaaataattg atgaaataat aatggaagaa aaaggccata tttatgaaat tgttgtagca
    26401 gaatattcta cgcaattaat agagctatca tctgatgaat tgaaattcgg accaaaattg
    26461 ctgaataaca aaaatgaata ttttataaaa aaatggcaaa gagagttaga agcactttat
    26521 catattaaat cgaaactcaa tactgaacaa catcgtcaac gtttagctca aattaatgac
    26581 gaaattgctg taattgaaag ggtgttatag taatgaaaat agctgattta atgacattgt
    26641 tagatcatca tgtgccattt agaactgctg aatcttggga taatgtagga ttgttaatag
    26701 gtgatgaaga tgttgaagtt actggtgttt taacagcatt agactgtacg ttggaagtag
    26761 taaatgaagc aatcgaaaaa ggttataata ctattattag tcatcatcct ctaatcttta
    26821 aaggtgtaac atcattaaaa gctaatggtt atggtttgat tattagaaaa ctaattcaac
    26881 atgacattaa tttaatagcg atgcatacaa atttagatgt aaatcctcat ggtgtcaata
    26941 tgatgttggc gaaggcgatg ggattgaaga acatttcaat aataaataat caacaagatg
    27001 tatactataa agttcaaaca tatataccta aggataatgt tggaccattt aaagataagc
    27061 ttagtgaaaa tggattagca caagaaggta attatgaata ttgtttcttt gaaagtgaag
    27121 gaagagggca attcaaacca gttggtgaag ctaatccaac aataggacaa attgataaaa
    27181 ttgaatatgt agatgaagtt aaaattgaat ttatgataga tgcatgtcaa aagtcatggg
    27241 ctgagcaatt aattaaacaa taccatccat atgaaacacc ggtatttgat tttattgaga
    27301 taaaacaaac atctctttat ggacttggcg ttatggcaga agtggataat caaatgacat
    27361 tggaagattt cgcagctaat attaaatcta aattaaatat cccaagtgtc cgttttgttg
    27421 gtgagtctaa tcagaaaatt aaacgtattg caattattgg tggttcaggt attggatatg
    27481 aatatcaagc tgttcaacaa ggcgcagatg tctttgttac aggtgatatt aaacatcatg
    27541 atgccttaga tgctaaaatt catggtgtga acttaattga tattaatcat tacagcgaat
    27601 atgtgatgaa agaaggttta aaaacgttac taatgaattg gttcaataca gaaaaaataa
    27661 atatagatgt tgaagcatct acaattaata cagatccatt tgaatatatt taaactaaaa
    27721 tctcgggatt tctaaatttt gaaatttcga ggttttaata tttttattta aaatagcaca
    27781 tttatacttt ataatagtaa agatgaacat ataaggaggc caaatcatgg caaaacatcc
    27841 attcgaacaa tttaatctag aatctagttt aattgacgct gtgaaagacc ttaattttga
    27901 aaaaccaact gaaattcaga atcgaattat tccaagaata ctaaagagaa caaatttaat
    27961 tggtcaatct caaacgggta cagggaaatc tcatgcattt ttattaccat taatgcagtt
    28021 aattgatagt gaaataaaag aaccacaagc aatcgtagtt gcaccaacaa gagaacttgc
    28081 acaacaacta tacgatgcag cgaaccattt aagccaattt aaagctggtg tttcagttaa
    28141 agtttttatt ggtggtacag atatagagaa agatagacaa cgttgtaatg cacaacctca
    28201 attgattata ggtaccccta ctagaattaa tgatttagct aaaacgggac atttacatgt
    28261 gcacttagca tcatatttag ttattgatga agcggatctt atgattgact taggattaat
    28321 tgaagatgta gattacatcg ctgcaagatt ggaagataat gcaaatattg cggtgtttag
    28381 tgctacaatt ccagaacagt tacaaccatt tttaaataaa tatttaagtc atccagaata
    28441 tgtagctgtc gacagtaaaa aacaaaataa aaagaacatc gaattctttt taatacctac
    28501 taaaggtgca gctaaagttg aaaagacttt aaatttaatt gatatactaa atccatactt
    28561 atgtattgtt ttctgtaata gtagagataa cgcaaatgat ttagcacgtt cactaaatga
    28621 agctggtatt aaagttggta tgattcatgg tggtttaacg ccacgtgaac gtaaacaaca
    28681 aatgaaacgt atacgtaatt tagaattcca atacgttata gccagcgatt tagcatctcg
    28741 tggtattgat attgaaggtg ttagtcatgt catcaatttt gatgtgccaa atgatattga
    28801 cttctttaca catagagtcg gccgaactgg ccgtgggaat tataaaggtg tagcaattac
    28861 gctttatagt cctgatgaag aacacaatat ttcattaata gaagatcgcg gttttgtttt
    28921 caatactgtt gatattaaag atggtgagtt aaaagaagtt aaagcgcaca atcagcgtca
    28981 agcaagaatg cgcaaagatg accatttaac taatcaagtg aagaacaaag ttcgaagtaa
    29041 aattaaaaac aaagttaaac caggttataa gaagaaattt aaacaagaag ttgaaaaaat
    29101 gaaacgtcaa gagcgtaaac aatttagtaa gcagcaaaat agacaaaaac gtaagcaaaa
    29161 caaaaaaggt taggtgtgtt ttatgttatt aggatcacat gtttcaatga gtggtaaaaa
    29221 gatgttagaa ggttctgcta tagaagcgca tgaatatggt gaaacaacat ttatgattta
    29281 tactggtgca cctcaaaaca cgcgccgtaa aagtattgaa gatttaaata taactaaagg
    29341 tcatgaagtg atggaaaaat atggcttatc taatattgtt gttcatgcac catacatcat
    29401 taatattgca aatacaacca aaccagaaac gtttaatctt ggtgtagatt tcttgcaaca
    29461 agaaattgaa agaacgcaag ctataggcgc taaagatatt gtattacatc caggtgcaca
    29521 tgttggagct ggtgttgatg ccggaattaa taagattatt gaaggattaa atgaagtttt
    29581 aacgaatgac aataatgtac gtattgcgct tgaaacgatg gcaggtaaag gtacagagat
    29641 tggtcgctcg tttgaagagt tagcacgtat tattgatgga gttcataata acgaacgttt
    29701 atcagtatgt tttgatacat gtcatacgca tgatgctgga tataatgtga aagaggactt
    29761 tgatggcgta ttaaatgaat ttgataaaat cattggagtc gacaggatta aagttgttca
    29821 tgtcaatgat tctaaaaatg accgtggcgc tcaaaaagac cgacatgaaa atattggctt
    29881 cggttacatt ggttttgatg cattaaatta cattgtgcat catgatagtt ttaaagatat
    29941 tccgaaaatc ttagaaacac cttatgttgg tgaagataag aaaaataaaa aaccgccata
    30001 taaacttgaa attgaaatgt taaaacaaca gcattttgac ccagaattaa aaaataaggt
    30061 tatgcaacaa taaaatgata atgctctctt caagaaatgc gataaataaa acttgaaggg
    30121 ggcatatctc tttattttgt ctaattttga gtcgtaaaca ttactgttta catatgttac
    30181 taggagttgt atagttgaat gcgaggtgag aatacgatga caccagtctt tgaattgaaa
    30241 aatgtcaatt actactatga tcataaaaaa gtgttagaaa atataaacat taaaataaat
    30301 aaaggtgaat ttttagcaat tgttggacca aatggtgctg gtaaatcaac attattgaag
    30361 ttgattctag gattattacc tttacaaagt ggtgagattt ttgttgaagg tattgatttt
    30421 aaaaataaga aaacatcaat taaattaagc tatgtatcac aaaaagcaaa tgcctttaat
    30481 tcaggtttcc cagcaagtgt taaagaagtt gttttaagcg gattaacaaa gacaaaacgt
    30541 cttttccaaa catttaatag caaagataat gaaaaagtga ttaaagtact agaaagactg
    30601 aatataagtg atttaattca taaaaatata gcagaattat caggtggtca acaacaacgt
    30661 gtaatgattg ctcgagcatt gatttcagaa cctgcagtat tagtacttga tgaaccgacg
    30721 aatggtattg atgcaaaaca tgtaagtgaa ttttataata ctttagatca attaaaacaa
    30781 gaaggtatca ctattatctt agttactcat gatattggtg ttgtagcaga tactgctact
    30841 gaagtagcat gtttaaataa gcatttgcat ttccatggta caactgatga gtttaaatca
    30901 cttgatgaag ttgaaatttc aaaaatttat ggacatcctg tacgttttgt cgatcatcag
    30961 cataatcgag aatgttgtaa ttaataaaga tttgagttta tttaaaatgg aaaggcgtgt
    31021 cacaattgat agatgcatta ttgaattttg attttatgag gtactcttta attagtggta
    31081 tcttgatagg ttttattgcg cctctaatcg gtgcttttat cgttgttaga cgactatcac
    31141 ttatagctga tgctctaagc catgtaactt taggtggtat atccttcggt atgtttttac
    31201 ttactattat gccaacacta gtatttatta atccaatgtg gtttggaatc ttattcgcaa
    31261 tagtaggtgc gctactaatt gaaaaattaa gaacgtcata cactgcttac caagaaattg
    31321 ctattccaat tataatgagt gctggtatcg ccttgagtgc aatcttcatt tcattagctg
    31381 atgggttcaa tcaagaaatt gttggattgc tatttggctc aataagcgca gtgaatataa
    31441 gtgatttaac aacgattatt gtcattgcca tcatagttgt tctgtttatt acattgtttt
    31501 ataaagaatt atttatattg tcatttgatg aggaatatag taaagtcatt ggcattccta
    31561 aatggattca atttttattt attgttattg tagcgatggt tatttctgcc tcaatgcgtg
    31621 ttgtagggat actgttagta agtgcgttaa tcacgttacc aattgcaata agtatgcgaa
    31681 taacaaaaag ttttaagcaa ttgatattac taagtgtatt tttaggtgaa ttatcagtaa
    31741 tattaggctt agtcctagct ttctatatgg acatttctcc gggtggtgta attgtagttc
    31801 tactagttat tttacttatg attacaatgg cttatcagaa aatgcgaatg aagtttaaaa
    31861 agggagctaa tatcaatgaa tacaaatgat gctattaaaa ttttaaaaga gaacggttta
    31921 aaatatacag ataaacgtaa agatatgtta gatatttttg tcgaagaaga taagtatata
    31981 aacgcaaagt atatacaaca agttatggat gaaaattatc ctggaatttc attcgacaca
    32041 atatatagaa acctgcactt atttaaagat ttagggatta ttgaaaatac agaacttgat
    32101 ggtgaaatga agtttagaat tgcttgtaca aatcatcatc atcatcattt tatctgtgaa
    32161 aagtgtggag atacaaaggt aatagattat tgtccaatag atcagataaa attatcacta
    32221 cctggtgtta atattcacaa acacaaactt gaagtttatg gtgtatgtga gtcttgccaa
    32281 gattaatata aagaaatgag atttatgcac atttggtcag atgtatgcat aaatctcatt
    32341 ttttaaatta tagaagataa aggttcattt catactccgt tcgtatgatt ttatggcaaa
    32401 tctgttaact ttttatatac aaaat
//
LOCUS       DAKSQE010000020        29663 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.020, whole genome shotgun sequence.
ACCESSION   DAKSQE010000020 DAKSQE010000000
VERSION     DAKSQE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 29663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29663
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.020"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(180..422)
                     /gene="rpsR"
                     /locus_tag="O0V33_000771"
     CDS             complement(180..422)
                     /gene="rpsR"
                     /locus_tag="O0V33_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCX8975437.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            complement(474..977)
                     /gene="ssb"
                     /locus_tag="O0V33_000772"
     CDS             complement(474..977)
                     /gene="ssb"
                     /locus_tag="O0V33_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCX8975438.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            complement(998..1294)
                     /locus_tag="O0V33_000773"
     CDS             complement(998..1294)
                     /locus_tag="O0V33_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCX8975439.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            1538..1729
                     /locus_tag="O0V33_000774"
     CDS             1538..1729
                     /locus_tag="O0V33_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975440.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIINASKIIPFF"
     gene            complement(1815..2912)
                     /gene="ychF"
                     /locus_tag="O0V33_000775"
     CDS             complement(1815..2912)
                     /gene="ychF"
                     /locus_tag="O0V33_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCX8975441.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(2924..3127)
                     /locus_tag="O0V33_000776"
     CDS             complement(2924..3127)
                     /locus_tag="O0V33_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCX8975442.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIVSQDDTQR"
     gene            complement(3157..4038)
                     /locus_tag="O0V33_000777"
     CDS             complement(3157..4038)
                     /locus_tag="O0V33_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCX8975443.1"
                     /translation="MDQVMSIISSLFEPLTKIETYESVATKLAMIIIYIIVALIVIKI
                     LDKMIAQGFKIQNKSKKGNKKRSKTLISLVQNVVKYIVWFIVVTTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFENQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVDENIDNVEKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAFEDTRVILRVSAETIPGEGFSGARIIRKEVQKMFLQEGI
                     KTPQPIMTPFNHSENGV"
     gene            complement(4192..5037)
                     /locus_tag="O0V33_000778"
     CDS             complement(4192..5037)
                     /locus_tag="O0V33_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCX8975444.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIADLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGAKVDISIKKSVGKISFEFESQDDFVRIIKQLN
                     RKYGK"
     misc_binding    5266..5635
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5701..6804
                     /locus_tag="O0V33_000779"
     CDS             5701..6804
                     /locus_tag="O0V33_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCX8975445.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQKLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAEKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     gene            6801..7961
                     /locus_tag="O0V33_000780"
     CDS             6801..7961
                     /locus_tag="O0V33_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HCX8975446.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAMSE
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTATHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     AALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTLER"
     gene            7930..9771
                     /locus_tag="O0V33_000781"
     CDS             7930..9771
                     /locus_tag="O0V33_000781"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HCX8975447.1"
                     /translation="MSQFLTHLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAYLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLEKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKHTHFNIAGAFNPNVRKLDGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            9768..11996
                     /gene="metE"
                     /locus_tag="O0V33_000782"
     CDS             9768..11996
                     /gene="metE"
                     /locus_tag="O0V33_000782"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCX8975448.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGLGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            12039..12791
                     /locus_tag="O0V33_000783"
     CDS             12039..12791
                     /locus_tag="O0V33_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HCX8975449.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIETFENKDANGQQHAPGWG
                     LDALKCLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(12874..14055)
                     /locus_tag="O0V33_000784"
     CDS             complement(12874..14055)
                     /locus_tag="O0V33_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCX8975450.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEINEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPIVVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPAIMGIAPVDAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            14595..15728
                     /locus_tag="O0V33_000785"
     CDS             14595..15728
                     /locus_tag="O0V33_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HCX8975451.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFATVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIASTGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLSSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISYSLGIL
                     VMSYIVSLLFKGHDHVMIISVSVATFCIMLIYFKNQRLRNKHE"
     gene            complement(16097..16726)
                     /locus_tag="O0V33_000786"
     CDS             complement(16097..16726)
                     /locus_tag="O0V33_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HCX8975452.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMICASILLLISFVVFFFIY
                     GMVSGASALEFEKISTGGWLVVIVYAVIGIVITSVSYISSIKIFNKQEL"
     gene            complement(16726..17568)
                     /locus_tag="O0V33_000787"
     CDS             complement(16726..17568)
                     /locus_tag="O0V33_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8975453.1"
                     /translation="MTTLLNLDRVNKQYKSSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSITFFGEEHAIDDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYMTWNSQKFFDLIKYFELPLQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMELLEDFVAQGGGILISSHISEDIEQLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTEDKDVEIPKHLIIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            complement(17593..18306)
                     /locus_tag="O0V33_000788"
     CDS             complement(17593..18306)
                     /locus_tag="O0V33_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HCX8975454.1"
                     /translation="MKILRYIGYLLLGGLVGGIIGGILGNFDGLGIENLTFATHNNVV
                     VISIIATIIIILIEIIVLMNQRRALKYKRLVDEEVDNEETDQYELLANRHVLNGSILS
                     ILQTVIALLVLLIFVVGQAEVNGILLFLIPFFASAIFNTQFTLFNRRFDDRMPKIADK
                     NYTEKRLEILDEGEHHIELIALFKTYAINLSILILAIIFIGSYSIATGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            complement(18303..18506)
                     /locus_tag="O0V33_000789"
     CDS             complement(18303..18506)
                     /locus_tag="O0V33_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCX8975455.1"
                     /translation="MRNRLKELRARDGLNQTQLAKQAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNEKVETIFIIEEDEV"
     gene            18746..19135
                     /locus_tag="O0V33_000790"
     CDS             18746..19135
                     /locus_tag="O0V33_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCX8975456.1"
                     /translation="MAFKLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSVKSNHPVAPSSNQSIVQDVLTRHQQGQTDFETFCDEMAQAGIYK
                     WHIDIQAGTCTYIDLQEQAIISELIPQ"
     gene            19234..19458
                     /locus_tag="O0V33_000791"
     CDS             19234..19458
                     /locus_tag="O0V33_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HCX8975457.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            19475..20176
                     /gene="tatC"
                     /locus_tag="O0V33_000792"
     CDS             19475..20176
                     /gene="tatC"
                     /locus_tag="O0V33_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HCX8975458.1"
                     /translation="MVHFSELRQRLVKILLSFVIAVIVVYVSSFWWMTPFITYITRAH
                     VSLHAFSFTEMIQIYVMIIFFIAFCFISPVMFYQLWAFIAPGLHNNERQFIYKYSFFS
                     VLLFCAGVAFAFYVGFPMIIQFALKLSLTLNISPVIGFKAYLIELIRWLFTFGILFQL
                     PILFMGLAKFDLIDTYSLKHYRKYIYFACFVLASIIAPPDITLNILLTLPLILLFEFS
                     MFIVKFTSRNDLSSQ"
     gene            complement(20522..22234)
                     /locus_tag="O0V33_000793"
     CDS             complement(20522..22234)
                     /locus_tag="O0V33_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCX8975459.1"
                     /translation="MKHYLTKIIATIITLVMVFSFGLLKSQAAEQQSISDVYSVITDA
                     KSALSNNSISKDNKLKAIEQVVSAVKKLSLEDNSEGNAVKSNVRKLEDAKANDDQKDT
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDK
                     KSGTSANDQYHVSQLNDELEKAIKAIDNNQLSDADAALTHFIEIWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKAHVKDGLVDINNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRNVKDKKGTASVIGGAIAGLVLSIVLAITFVETLGNSGMLRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYTNAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFVIGIALAIVILIVFALLFRFIVRLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQAAYIMVVAILIF
                     KFKK"
     gene            complement(22215..23444)
                     /gene="efeB"
                     /locus_tag="O0V33_000794"
     CDS             complement(22215..23444)
                     /gene="efeB"
                     /locus_tag="O0V33_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCX8975460.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQSGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDTVGKPSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETPRNLMAFKDGTINPRKSNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATQQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            complement(23441..24295)
                     /locus_tag="O0V33_000795"
     CDS             complement(23441..24295)
                     /locus_tag="O0V33_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975461.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDITPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNKLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
     gene            complement(24560..25105)
                     /locus_tag="O0V33_000796"
     CDS             complement(24560..25105)
                     /locus_tag="O0V33_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCX8975462.1"
                     /translation="MFGMKVNEQIRLKILEAHDTEALFNLVNRSRNSLREWLPWVDAT
                     EQPSDTRAFIKSGLLQFADGNGFQCGIWYEGTLVGVIGLHEINHMHRKTSLGYYLDKQ
                     FEGHGIMTQAVEALIKYCFDEIELNRIEISAAVNNEKSQAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLKSEYNEG"
     gene            25465..26460
                     /locus_tag="O0V33_000797"
     CDS             25465..26460
                     /locus_tag="O0V33_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HCX8975463.1"
                     /translation="MASLKNKHFMIGLSLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFKGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSRLLAIDVGVVI
                     FSIVMMLFVNKLLRGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSVLLNIL
                     NTVSTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
     gene            complement(26520..27086)
                     /locus_tag="O0V33_000798"
                     /pseudo
     CDS             complement(26520..27086)
                     /locus_tag="O0V33_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            complement(27100..28161)
                     /locus_tag="O0V33_000799"
     CDS             complement(27100..28161)
                     /locus_tag="O0V33_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCX8975464.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPFKGEKVSYEQRINEIIEA
                     SKLADEAGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRASRTGVFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNTLPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDASPKSLPVSTASLFYTAKTTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSSDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNDIIPAIKKHLSK"
     gene            complement(28175..28342)
                     /locus_tag="O0V33_000800"
                     /pseudo
     CDS             complement(28175..28342)
                     /locus_tag="O0V33_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            28396..28680
                     /locus_tag="O0V33_000801"
     CDS             28396..28680
                     /locus_tag="O0V33_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975465.1"
                     /translation="MTEFTPFFDGSNQLNYLKFVILRFHGVSKRKICKMYNFAYFRIL
                     EVCEMAKKNDYRFTYKDYKFLKQYGVSNTFICKMYHISIEDLEFFEVMNR"
     gene            28831..29151
                     /locus_tag="O0V33_000802"
     CDS             28831..29151
                     /locus_tag="O0V33_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HCX8975466.1"
                     /translation="MKPVLDIKKTLGQLNFLGVDEKYKYENGERTDKTVYAYKLASQE
                     QGEQITVKTPNKVELDYLQECELVEPDVKMYVQMSGDFGTIAYSWNAEDIKVVGSNSA
                     LKQK"
     gene            29165..29467
                     /locus_tag="O0V33_000803"
     CDS             29165..29467
                     /locus_tag="O0V33_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975467.1"
                     /translation="MDYYTADRLYRYTNSSNLSEPILNYVASRINWGDKVSLMTLAKE
                     IQSKFNDSYVKENTVKGRPKIYADLCLLCMSLSEAGHGRMLQVNLEDCIYIGDIDV"
BASE COUNT         9477 a         5655 c         4485 g        10046 t
ORIGIN      
        1 tcaccaaact attatatgag attaatatct cgcaaagacg gacaattatt tgtccgtttt
       61 tttaatttta atcatcaaac ttttcacact ctgcgaaaaa attgcgaaat ttttataaaa
      121 ataaacacaa aaacaaccct ctagcctgat tgctaaaggg ttttgttata cgttatatat
      181 tattgttctt ctttaacata tggtaataat gccatatgac gagaacgttt gatagctgta
      241 gtcaacatac gttgatattt agctgaagta ccagttacac gacgtggtaa aattttaccg
      301 cgttctgaga taaaacgttt taataattca gtgtctttgt agtcgatatg tgtaatacca
      361 tttgctgtga aatagcatac ttttttacga cgacgtccgc ctcttcttgg tccacctgcc
      421 atgattgtgt gcctcctttg ataatttttt cgctttaatt tcgttaattt ttattagaat
      481 ggtaagtcat catcacttat atcaatcggt ccgtttgcat ttgcaaatgg attatcagat
      541 tgtttcgtgt ttgatgaatt attgtacgaa ttgttttgtc ctgattgttg accaccgaat
      601 ccttgaccgt aatcttggaa ttcattttgt tgacgttggc caccattttg ttgtgcattt
      661 ttaggttcaa ggaattgaac gctatcacac acaacttcag taacaaacac acgacgacct
      721 tcttgatttt cataattacg ggattgtaag cgaccatcta caccagctaa actaccttta
      781 gataaatagt tatttacatt atctgcttgt cttctaaaaa caacacagtt aataaaatct
      841 gcttcgcgct ccccttgagc attcgtgaac gtacgattta ctgcaagagt gaatgtcgct
      901 acactcacac ctgagggagt ggttctgtat tccggatctt tcgttaaacg acctactaat
      961 acaactctat ttagcattta aacgccccct ctaattatta cttgtcttcg tcttcacgaa
     1021 taaccatgta acgaatgata tcgtcactga ttttagctag acgttggaat tcgtcagtag
     1081 ctttgttgtt atcagattta acacgtacga tgttgtagaa gccatctttg aaatcattga
     1141 tttcataagc taggcgacgt ttaccccagt cttttgcttc taaaacttct gcaccttcag
     1201 tagctaagat accgttgaaa cgttcaacta acgctttttt agcatcttcc tcaatgtttg
     1261 ggcgtacgat gtacataact tcatatgttc tcattttata tttgcacctc cttgtggtct
     1321 atacggctta tcaatcttaa aacagataag caaggaataa ttttcattac tcacaataaa
     1381 gaattatatc atgcgccatt actttttaca ataataattc aaactactct tcatatcatt
     1441 tttgacatta attcatttga aattttctat gatattttta aaaatacact tcacaaaagc
     1501 gaacacatgt tctataatga ttgtgaggtg gtaaggaatg aatttaaata ttgattggtc
     1561 taaagatttt caagaattcc aagagatact taatagtggc attcatcctg aatggcttta
     1621 ttgtgcaaag gctaatcttg ttttagagcc tgcttatact ggcgaaggca aacaattttt
     1681 cagcactcaa gatattatta acgcgagtaa aattattcca ttcttttaat aaattatacg
     1741 cataattaaa agaggcgaac aattgattcc gcctcttcta catatatatt atttaattaa
     1801 cttcactcta tcgtttatac attaaatctg aaatgaacga tatcgccatc ttgcataata
     1861 tattctttac cttctaatcg ttgtctgccc gcttctttag cgccactttc gccaccatat
     1921 tgtacatagt cgtcataact tgttacttcg gcacggataa atccacgttc aaaatcagta
     1981 tgaatgatac cagcacattg aggtgcagtc ataccttgtt taaatgtcca agcacgtact
     2041 tcttgcacac cagcagtaaa atatgttgat aatcctaata attcataagt tgttctaatt
     2101 aatcgatcta atcctggttc ttcgatacct aaatcttcta agaacatttc tttatcttca
     2161 tcatctaatg tagcaatttc ttcttcaatt tttgcactaa taacaatcac ttcagagtct
     2221 tcttgcgctg catattcacg aatcgctttt actttatcat tatcatcatc accaatttca
     2281 tcttcaccaa cattagcgat ataaagcatt tttttagaag tcagtaattg cgcttgattc
     2341 acccattttt gatcttcttc attaaagtca atactacgag cgggtttacc attttctaaa
     2401 gcttctttaa tagttgttaa aatacgcact tccatttccg cagtcttatc tttttgacgt
     2461 gccaattttt caattctagg taagcgtttt tcaactgatt ctaagtctgc aagtactaat
     2521 tccatattga taacttcgat gtcatcaatt ggatcaactc gaccagctac atgtgttaca
     2581 ttatcatcgt caaaagcacg aacaacctga caaattgcat ctacttctct aatatgtgat
     2641 aagaatttat tacctaaacc ttcaccttta gaagcacctt taacaattcc ggcaatatct
     2701 gtaaattcaa atgtagtagg taatgttttt ttaggttgaa ccatttcttc taatttaagt
     2761 agtcttgcat ctggcacttc tacaataccc acgtttgggt ctatagttgc gaacggatag
     2821 tttgctgcca aggcacccgc ctttgtaatt gcattaaata atgttgattt accaacgttt
     2881 ggcaagccaa cgatacctgc tgttaaagcc atgaatcatt ctcctatctt tgtgtatcat
     2941 cctgagatac gatgattttt tttagttttt tatcaaacgt ttgacgtgga atcataatgc
     3001 ttcgttgaca attttcacac ttaattctaa tgtcagcacc cattctaata attttaaaac
     3061 gattggttcc acacgcatgt tgttttttca tttctactat atcatttatt ccatattttg
     3121 acgccattaa atatgacctc catgtattat aaactactaa acaccgtttt cactatgatt
     3181 aaaaggtgtc attattggtt gtggtgtctt aataccttct tgtaaaaaca ttttttgtac
     3241 ttctttacga ataatacgcg caccagagaa accttcacct ggtatagttt ctgctgaaac
     3301 ccttaatatt actctagtat cttcaaaggc atcaatacca atcacaacag gatcacttac
     3361 aaataaataa tatttactac gcagagatgt aaataatttg tttaatttct tctcaacatt
     3421 atcaatattt tcatctacag atactggaat tttaacaatt gctgttccat ttgtaattga
     3481 gtaattcgtt atttcaccca tactactatt tggcaagatt gtaagttccc cagatattgt
     3541 attaatgcgt gttgaacgga gtccaattga tttaactgtt ccctctgcta cagttgtacc
     3601 accgttgttt atcttaacat aatcacctac atcaaactgg ttttcaaata taataaagaa
     3661 tcctgtaatt acgtctttaa ctatagtttg agcaccaaaa cctactgcta ggcctacgac
     3721 accagcactt gcaattactc cttcaacact aatgccaaat ttacttaaaa tggtagttac
     3781 aactataaac cacacaatat atttaacaac attttgcacc aatgatatta atgttttcga
     3841 acgtttttta ttaccctttt tactcttgtt ttgtatttta aatccttggg caatcatttt
     3901 atcaagtatt ttaattacga ttaatgcaac gataatatat ataattatca tcgctaattt
     3961 agtagcgaca ctttcatatg tttcaatttt agttaatggt tcgaatagcg atgaaataat
     4021 actcataact tgatccataa ttagcgttac tcccttctat atagtctcaa ataattttat
     4081 taatattatt gtatgcttcc aacctactga agtcgcatca tcttcaaaga atatagataa
     4141 ctatcgatat ttctatagat tcacaaagtg atattatata aaattgtgta actatttacc
     4201 atacttacga tttaattgtt taatgattct cacaaagtca tcttgcgatt caaactcaaa
     4261 tgagattttg ccgactgatt tttttatcga tatatctact tttgcgccat actgttctcg
     4321 caactgtcgt tcttgttgct ttataaattt aggcttagtt atatctactt tgtctgtttc
     4381 cgactttgac gaaacatttt ttaattcatt aacatggttt tctaaatatc taacactcca
     4441 cttttcttta acaacccgtt tcgctaacct aagcatttgt tgttcatctt taattgccaa
     4501 taacgttcgt ccatgtgcac ttgtcagtcg cccatctttt accatgtcag caatcttttt
     4561 cggtaaatgt aataacctca acatattcgc tatatacggg cgcgacttac tcaatcgctt
     4621 cgctacttct tgttgcgtaa ttttcaaatc tgtcatcaaa cgttgataac tttcagcttc
     4681 ttcaatcgca tttaagtctt ctcgttgtaa attttcgatg accgccagtt ccatcatatc
     4741 ttcatctgtt aaatctttga taatcgctga tacgtatttt agatcagcaa ttttcgaagc
     4801 tctaaacctt ctttcaccaa ctacaatgta ataaccttga actgtttttc taagcacaat
     4861 tggttgcaaa ataccatatt gcttaattga atctgccaaa tcatttaaat gattttcatc
     4921 gaaagttttt cggggctgat acggattcgg tttgatatcc gatatatcta tttgcttaat
     4981 ttgttcatct tttgttttag taatacgttg atcttcactt tttgacaatt cactcacatc
     5041 actttttatt tgataacaac agttaaatat attttatgta ttctaaatga gtcagacaac
     5101 cattaaccac actttattta aaaaataatt catttttcaa cttcaagata ccaattacaa
     5161 tttcacaaac aaattaactt ttaaatcaaa attttctgaa taattgttga caaatctttt
     5221 tttactctgt aagctaatta atatcttata acagtttaat gaaacgtaaa cacaataaag
     5281 aggaaagtaa aacacaccct gcttatacag agagtcttta gtagctgaga gaagattttg
     5341 aaagcgtgtt tgaaaatggc cttggagtgt tgatgccaat atgaggtgtc tacgggttcg
     5401 cccgttatag cgatacagta ttaacattga tgttaaatgg cgtactggat tctttacgca
     5461 cgattttttg ttaataagta tgggatagca cattactgta tccttactta acgacttaaa
     5521 ttgtgataat tgttcagtaa gcatatttac ttttaatgcg tactgaataa ggttatttca
     5581 gcgatggaat aacaaataaa ggtggtaccg cgaaacataa gctttcgtcc tttttatccg
     5641 attcattcgg gtacgaagga cggaagcttt tttatttttt cttactagga gggtcttaat
     5701 atgaaggata cacagttagc ccaaatcaca ttaactgatg attcaaccgg tgctatagcg
     5761 aatccaatcc atttatctac tgcctacaag catccaaaac taggacaatc gacaggattt
     5821 gattatacac gtactaaaaa tcctacacgc tcaacatttg aaacctgttt tgccaaactt
     5881 gagcatggta ttgcatcatt cgctacatca agtggaatgt cagccattca attaatatgt
     5941 aatttattta aaccacacga tgaaatttta gtttcattcg atttatacgg cggcacattt
     6001 agattatttg aattttacga gcaacaatac gatatcaaat ttaagtacgt tgattttaca
     6061 gattatgaac aagttgaaaa agaaatcact gataaaacag tagcattatt cattgaacca
     6121 atatccaatc cacaaatgat tgctattgat gtaaagccgt actatcaact ttgtaaagct
     6181 aaaggcttat tgtcaattat cgacaatact tttttaacac cttatctttc aacaccatta
     6241 gcagaaggtg ctgatatagt cttacattca gctacgaaat atattggtgg acataacgat
     6301 gtactagcag gtgtcgtaac cgtcaaagat gaatcactcg cgcaaaagtt gtttgatttt
     6361 cacaacatga ctggcgcaac actatcacca atagatagtt atttgttgtt acgtggactt
     6421 aaaactttgc atttacgcat tgagcgtgcg caatcaaacg caagaaaact tgctgaaaaa
     6481 tgtcagtcac ttcaagcaat tgacgaagta ctatatagcg ggcaaactgg catgcttagt
     6541 ttaagactta acaaggccta tagcgtcgct aaattattag aaaatttaga catttgcatt
     6601 tttgcagaaa gtttaggagg tactgaaaca ttagtgacct tcccttacac ccaaacacat
     6661 gttgatatgc cagatgctga aaaagataaa cgtggcattg atgagtattt aatccgctta
     6721 tcactaggtg ttgaaaatta tgaagacatc gaacgcgata tcatccaagc attagataaa
     6781 gctcagattg gagagattgt atgacacttt caaaagagac agaagtgata ttcgattggc
     6841 gtagaggtgt ggaatatcat tcagctaacc caccactcta tgattcttca acattccatc
     6901 aaacaagtct tggtggcgat gttaaatatg attatgcacg aagtggcaac cctaaccgtg
     6961 aacttttaga agagaagtta gcacgattag aacaggggaa attcgctttt gcttttgcat
     7021 caggtattgc tgctatttca gcagtacttt tgactttcaa atctggtgat catgtcatct
     7081 tacccgatga tgtatatggc ggtacttttc gcctcactga gcaaattttg aatcgattta
     7141 atattgaatt tacaaccgta gatactacca agctcgaaca aatcgagggt gccattcaat
     7201 caaacacaaa attaatttat atcgaaacac catcgaatcc ctgttttaaa attactgata
     7261 tcaaagctgt ttctaaaata gccgaaaagc atgaactact ggtagctgtg gacaatacat
     7321 ttatgacacc gttaggtcaa tcacctttat tacttggcgc tgatatcgtc attcatagtg
     7381 ctacaaaatt tttaagtgga catagcgatt taattgctgg tgctgtcatt actaataatg
     7441 aagcaatgag tgaagcactt tatttaatac aaaatggtac gggcaatatg ttatctgctc
     7501 aagatagctg gacgcttgct aaacatttga aaacatttcc aatcagattt aaacaatctg
     7561 tcgaaaacgc gcaaaaaatc gtgtcatttt taataaagca agatgaaatt tcagaagttt
     7621 attatccggg acttactgct actcatttag aacaagctaa aaatggcggt gccgttattg
     7681 gctttcgttt agctgatgag tctaaagcac aacaatttgt cgatgcactg acattaccac
     7741 tcgtttcagt gagtctgggc ggtgttgaaa cgatactttc acatccagca acaatgtctc
     7801 acgctgcact acctgaagaa gtgagacaag aacgtggtat cactttcggt ctgttccgat
     7861 taagtgttgg tctcgaagat cctgatgaac tcattgcaga catcaaatac gcattaaagg
     7921 aggcattcaa tgagtcaatt cctcacacac ttgaaagata atgttttagt agctgatggc
     7981 gctattggaa ctattttata ctctgaaggt ttagacacct gcccagaagc atataacctt
     8041 agccatccag ataaagttga acgtatccat cgttcatata ttgaagccgg tgctgatgtc
     8101 attcaaacca atacttatgg tgcaaatttt gaaaagttaa aacgattcgg tcttgaagat
     8161 aaagttaagg ctatacatca agcagccgtt cgcatcgcaa aaaaagcagc aaataaagat
     8221 acgtatatat taggcacagt tggtgggttt agaggtatca aacaagagga tatcagctta
     8281 caaactattc tttatcatac tgaaattcaa atagacacct tgattgaaga aggtgttgac
     8341 gcgctacttt tcgaaacgta ttacgaccta gaagagctaa caaatgtcat ttcacgaacg
     8401 agaaagaaat acgacattcc aatcattgct caattaaccg cttcaaacac aaattactta
     8461 gttaatggtc aggcaatcaa tgaaggatta aaacaactcg ttcaatgtgg tgcaaacatc
     8521 gtgggactca attgtcatca tggcccacac catatgcaag agtctttcac acatattgaa
     8581 ttaccagagc acgcatactt atcttgttat ccaaatgcca gcttattaga tattgaaaat
     8641 agtgaattta agtatagtga caatgcacaa tatttcggtc aagttgctca aaatctaatt
     8701 cgcgaaggtg ttcgtttaat tggtggttgc tgtggtacaa cgcctgagca catcaaattt
     8761 attaaagaat ctattcagac acttaaacct gttaatgaca aaaaagtgat tccgatacca
     8821 acgaaagcac ttttcaatcc atctcaaaat aaagttagac aatcattaac atctaaggtt
     8881 caagaacgtc caaccgttat tatcgaattg gatacaccga aacatttaga cacggataga
     8941 ttttttgaaa atatcgctaa acttgaaaaa gctaatgtag atgcggtaac actcgcagat
     9001 aattcattgg caactgtcag aattagcaac attgctgctg caagcttaat taaacaatat
     9061 tacaatattg aaccactcgt acatattaca tgtcgagacc gaaacttaat cggcttgcag
     9121 tctcatttac ttggattatc actcattggc gttaacgaaa tattagccat aactggtgat
     9181 ccttcaaaag ttggtcacct acctggtgca accaatgtct atgatgttaa ttctaaagga
     9241 ttaactgaac tcgctctaag atttaatcaa ggtattaaca ctgacggtga tgcgctgaag
     9301 aaacatacac acttcaacat cgctggcgcc tttaacccta atgttcgtaa attagatggt
     9361 gccgtaaaac gactagagaa aaagatagaa agcggaatgt cttattttat aacacaaccc
     9421 gtgtacagca aagagaaaat cattgaaatt taccatgcca ctaagcactt gaacaaacca
     9481 tttttcatag gcattatgcc tatcgcaagt tacaaaaacg cactcttttt gcataatgaa
     9541 gtgccaggaa tcaagatgtc agatgaaatt ttacaacaat ttgaagcagt taaagatgat
     9601 aaagccaaaa ctcgtgaact aagccttaag ctttcaaagg atttaatcga tactgttcat
     9661 gaatatttta atggtttata cattatcaca ccgtttcaaa atgtcgaaga ttcattagaa
     9721 cttgcagcat actcaaaatc tattactgct cacaaggagg caatattatg acaacaatta
     9781 aaacatcaaa cttaggattc ccaagattag gtagaaaaag agaatggaaa aaagccatcg
     9841 aaagttattg ggcaaagaag atttctaaag aagaattaga tcaaacatta actgatctac
     9901 ataaagaaaa tttattatta caaaagtact atcacttaga tagcatccca gttggtgatt
     9961 tctctttata tgaccacatt ttagatacgt cattattatt caacatcatc ccagaacgtt
    10021 tccaaggaag aactatcgat gatgacttat tatttgatat agctcgcggt aataaagacc
    10081 atgttgcaag tgcacttata aaatggttta acactaacta tcattacatc gtacctgaat
    10141 gggacaatgt tgagcctaaa gtgagtcgca atgtattatt agatcgcttt aaatacgcgc
    10201 aatctttaaa tgttaatgct caccctgtta ttgttggtcc gattactttt gttaaattat
    10261 caaaaggcgg tcaccaaact tttgaagaaa aagttaaaac gttattacca ctttataaag
    10321 aagtgtttga atcattaatt gatgcaggtg ctgagtacat tcaagttgat gagccaatct
    10381 tagttacaga cgacagtgaa agctatgaaa atattacacg tgaagcttat gactatttcg
    10441 aaaaagctgg tgttgctaaa aaattagtca ttcaaacata cttcgaacgt gcacatctta
    10501 aattcttaag ttctttacct gttggtggtt taggattaga ctttgtccat gataatggct
    10561 ataaccttaa acaaattgaa gctggagatt ttgataaatc aaaaacatta tatgctggaa
    10621 ttattgatgg tcgtaatgta tgggcaagtg acattgaagc taaaaaagtc ttaatcgata
    10681 aattgttagc acacactaat gaacttgtca ttcaaccatc atcttcatta ttacatgttc
    10741 cagtatcttt agatgatgaa acattagata caagtgttgg cgaaggctta agctttgcaa
    10801 ctgaaaaatt agacgaatta gatgcattgc gtcgcctatt taatcaaaac gatagtgtta
    10861 aatatgataa attaaaagca cgttatgagc gtttccaaaa tcaatcattc aaaaacttag
    10921 attatgattt cgaaagcgtg cgtacttcaa gacaatcacc attcgcgcaa cgtattgaac
    10981 aacaacaaaa acgtttgaac ttaccggatt taccaacaac aactattgga tcattcccac
    11041 aaagccgaga agttcgaaaa taccgtgcag attggaaaaa caaacgcatt acagacgaag
    11101 catatgaaac attcttaaaa aatgaaattg ctcgatggat taaaattcaa gaagacatcg
    11161 gcttagatgt attagttcac ggtgaatttg aacgtaatga catggttgaa ttcttcggag
    11221 aaaaattaca aggtttccta gtaactaaat tcggttgggt gcaatcatat ggttcacgtg
    11281 ccgtaaaacc accaatcatt tatggtgatg taaaatggac agcgccttta actgttgatg
    11341 aaacagttta tgcacaaagc ttaacagata aaccagttaa aggtatgtta actggacctg
    11401 taacaattct aaactggtca tttgaacgtg ttgatttacc acgtaaagtt gttcaagatc
    11461 aaattgcttt agcaatcaac gaagaagtat tagcacttga agctgcagga atcaaagtta
    11521 tccaagttga cgaacctgca ttacgtgaag gcttaccatt acgctctgaa tatcacgaac
    11581 aatatcttaa agatgctgtt ttatcattta aacttgcaac gtcttcagtt cgtgatgaaa
    11641 ctcaaatcca tacacatatg tgttattctc aattcggtca aatcattcat gctattcatg
    11701 acttagatgc tgatgttatt tcaattgaaa catctcgtag ccatggtgat ttaattaaag
    11761 actttgaaga tattaattat gatttaggta ttggtctagg tgtatatgat attcatagtc
    11821 cacgtattcc aacaaaagaa gaaattacta cagcaatcaa tcgttcacta caacaaatcg
    11881 accgctcatt attctgggta aaccctgact gtggtttgaa aacgcgaaaa gaagaagaag
    11941 ttaaagatgc attgactgtg cttgtgaatg ctgttaaagc taaacgccaa gaataatcgc
    12001 agcatttatc atataataaa ctaaaaacgg gtgattttat gacacaatat cctttatggc
    12061 aacaattaaa tactttaaaa caagcaactt gggttgattt aacgcatacg tttgatccgg
    12121 aaataccacg ttttagtgag tttgaaaaag gcgaagtttc aactttattc acggttaaag
    12181 atcatggttt ctatgtgcaa cgttggaata ttgtaacgca atatggtact cacattgatg
    12241 caccaattca ctttgtcgag aataaacgtt atttagaaga cattgattta aaagaattgg
    12301 tattgccgtt aattgtatta gacttttcaa cagaagtagc gaacaacaat gatttcatcg
    12361 taacacgtgc gcatatagaa gcatgggaaa aagagcatgg tactattgaa cctggcactt
    12421 ttgttgcact tcgtaccgat tggtcaaaac gttggcctaa tattgaaacg tttgaaaaca
    12481 aagatgcgaa cggacaacaa catgcaccgg gttggggatt agatgctttg aagtgcttaa
    12541 ttgaagaacg ccgtgttgaa gctgttggtc atgaaacatt cgataccgat gcatctgtgg
    12601 acgttgtaaa aaatggtgat ttagttggcg aacgttatat tcttggtcag gataaatacc
    12661 aagtcgagtt attaactaat ttagatcaat taccaactcg tggcgccatc atttacgcta
    12721 tcagccctaa accaaaagat gcacctgggt tccctgttag agcatttgca attaaaccat
    12781 caaatgacta atattaccca ataaatccat aaacaaataa aaacaggtta tgccaacatg
    12841 taattaatac tgttgtgata acctgttttc ttattattta tactttgata caactgcagc
    12901 gatagcttga ccgccaccaa tacacaatga tgttaaacca gtttcaactt catcattcag
    12961 ttgatgcaat aatgtcacta aaactctagc accagatgca ccaataggat gtcctaatgc
    13021 aatagcgcca cctttaacat tcaccttttc aggaggtaat tttaattcac gatcaacagc
    13081 taatgattgt gctgcaaatg cttcatttaa ttcaaataca tcaatatcac ttaattcttt
    13141 tttactacgt ttcaaagcct tttcaacagc gtcaactggg gcaataccca taatagcagg
    13201 atctacacca tgacttccaa agccatcaag cactgccaat ggttcgatat ttaattcttt
    13261 agctttgtct tctgacatga ctaacatcat cgcagcacca tcattgattc ctgatgcatt
    13321 acctgctgta actgtaccgt cttttttgaa agctggtctt aagcgactta atttttcaac
    13381 tgatacattt tcacgtacac cttcatcctt agtgacaacg attggttcac ctttacgttg
    13441 aggaatcgat actggaacta tttcactatc aaattcacca ttttgctgtg cacgtactgc
    13501 tttttgttgt gagtttacag caaatgtatc ttgttcttct cttgaaatac catattgctc
    13561 tactaaattt tcagcagtaa tacccatatg atattgatta aatacatctg ttaaaccatc
    13621 atataccatg ctatcaacca ttgattgatg tcccatttta aaaccaaagc gactgttgtt
    13681 gacaagcatt ggtgattgag acatattctc cataccgcca gctagcacga tgtcattttc
    13741 accagtcaca atagattgat atgctaattg aatcgacttt aacccagaac cacatacttt
    13801 attcactgta aatgcaggta ctgtttctgg caagccacct ttcatagcag caattcgtgc
    13861 tggattttgt ccttgtcctg cttgtagtac gttaccgatg atgacttcat taatctcact
    13921 tggattcaaa cccgtctctt taataatatg ttctattaaa gtcgcaccta aatcataggc
    13981 tggcacgtct ttaaacgcac ctccaaaaac gccaataggt gtcctgtatg ctgctgctaa
    14041 tacgactctc gtcatgtcat attcatctcc ttatgatata tacaatttta ttactaatac
    14101 gtctaatgaa ccaatacgcc actaacatcc aatttatgaa accaattgct acgtatgtta
    14161 cattttcatt cttgttatgc atttaacaaa tcatattata caatgatgta atttgtttgg
    14221 gcaatcggaa acgtaaacgg tttcacttta acatttcaca ctcttaaata tgctctatca
    14281 tcctcatcta tagtacaaaa ttatgctttg atttaccaca aattatcatt aatcatttcg
    14341 cgtaatgact ctaattaaat gctatctatc attgcatcgc tattcacagc ttgtttcacc
    14401 atcaatttca ccatctactt tttcaaacca tgacgtctct attcaaatat taaatcgctg
    14461 ataactgtta atgccgcaac cagctccctg tcacccctcc ttccatttca tcgtcaaatc
    14521 tgcccctatt cactagaaaa aataaatagt atacttagta aattaattac acaccttaaa
    14581 ggagttaaaa ttttatggtt aatgcattaa caccaaaaca tttagctgaa aagcgtgcag
    14641 gattccatga gttgttcttt gatttaattt tcgtctatgc cattcaaaaa attgcacatg
    14701 tgattttaac aacacaaaat ggttccattt cagctgattt atttttcaaa tacatcgtca
    14761 tgagtttgtt cttatggtta atgtggtctc atcaaacatt ttttacaaat cgatttggtc
    14821 aagttacatt caaagacgtc tcgtttatga tgtttaatat gtttatcatg gtcttcctat
    14881 ctaacagcct atatccggac tttgaaaaga cattcttccc attcttctta tgcgtggcaa
    14941 ttatgtattt aagtatcgga ttacagtatt tattacatat acgtacagga ttagattatg
    15001 gcgataagcg tacatgtcaa gcttttgcga ccgtagcctt tgtgatttca tttttatcat
    15061 ttctatcatt agtactacca caaagtatcc attacatccc tggattttta ggcgtattca
    15121 ttgcatcgac aggacttatt ccatttcaaa aatatctcgt attatcacct gttaatatga
    15181 tgcatttagt agaacgtttt tcattactga caattatcat ctttggcgag gtactggttg
    15241 gtttagcttc gtcatcattt agcattgatc attttagtta catttatata ttccaattta
    15301 tgattttaat tagtcttttc ggtgtatatt ggatcattac tgaaaattac attaatcata
    15361 aattgtcatc tatcggtttc cgtttatcgt atacgcattt attaatcaat atcgctttag
    15421 gtgtgattaa tgccgcaatt gtttttagta ataacaataa attaaatgac ttgtttgaaa
    15481 ttaatatgat gtatatcagt gttttaatat tctatattgg attatggtta atcacgcctt
    15541 atttccataa cgaattgaca aatgcgaagt atatttcata ttcactaggg atactcgtta
    15601 tgtcttatat tgtcagcctt ttatttaaag gtcacgacca tgtaatgatt atctcagtat
    15661 cagttgcaac cttctgtatc atgctgattt attttaaaaa tcaacgtcta cgtaataaac
    15721 atgaataaat agtatttacc tcggtcaaag tatttgaact gaatccaaaa gtgtcgaact
    15781 tcacattata ctttgtgaca aacttcagtt atgatacatg atcgaggttt ttaaatattt
    15841 ccgaggaaat cgttagccta tacaatcatc aaatgatact cataaataac atatattctc
    15901 taacccccac tatctaaaag gaaataattc cctcacaaca taaccacctc aaaacaaata
    15961 aatcaatcta gatatgtcac ctatacttat caccccgcat ttcattccac accaaaaaga
    16021 acagatgaca atcgtaaaga tttatcacct gctctaaata ttatttcaat gactagcttc
    16081 acaccaagaa tattgattat agttcttgtt tgttaaaaat tttaatagac gatatataag
    16141 aaacacttgt tataactata ccgataaccg catatactat gacaactagc catcctccag
    16201 tgctaatttt ttcaaattct aatgcagatg caccactaac cataccatat ataaagaaaa
    16261 agaccacgaa agaaataagt agtagtatag atgcacatat cataatgaca ttggagtttt
    16321 cagcgccaaa tttaaatgtc aatgggaaaa tgatagagta tgcccctatg ataccaaaac
    16381 tcattaaacc tgacataata ccaatcatca cactttgggt cacaatcgta gtcacaacta
    16441 atccaatcat taaacttgca ccgaataaga ttaaataaaa ggcaaagtat gacttaatat
    16501 aatcactacg tttaaccggt aaagtagata cataatacat ccatcttgag tctttttcat
    16561 gtttaatatt atcagtaata ggtgtaatta acataacccc agccattgcc gaactcatca
    16621 acggattaaa tactgcaaag tatcccgcag ctatgatagc gactataaaa taaatatatg
    16681 tttgctttct cgttgcataa aaactactta ggaacatacc tttcattata cttcacctcg
    16741 cattatgatt ttcgtagcgt catcaatgtg ttttaaaggt tctgcatttt caatttctgc
    16801 ataatcttta actaaaattt gatatttccc cttcgataat ctagaagcaa tgattaaatg
    16861 cttaggaatt tcaacatctt tatcttctgt cgtaacaatt ccatattgtg ctaaaagtat
    16921 atctttctgt tcagttaaaa tcattcgtcc atctttcata aacactaatt tatccgctaa
    16981 ttgttctata tcttcagaaa tatgagatga tattaagatg cctccacctt gcgcgacaaa
    17041 atcttctaat agttccatta cttcttctcg tccagaaaca tccatacccg cagttgcttc
    17101 atctaagatc aatagcttca catcatgaga aagcgcaatc gtcaaagcta tcttcattcg
    17161 catccctctt gaaaaagttt taattttagt ttgtagtggc aactcgaaat atttgattaa
    17221 atcaaagaat ttttgactat tccaagtcat ataaatagat tgaaatactt tatcaatatc
    17281 tttaatagtc aatttattag gtactctcaa atcatcaaac actacaccta tgtgttcttt
    17341 atattcgaca tcatcaatag catgttcttc cccaaaaaat gtgatactac cgttatcttt
    17401 atgtcgattg cctactagaa tattaattaa tgtcgattta cctgagccat ttttcccaat
    17461 taatccaact gtctcattgg tagaaatcgt taaagatgca tcttgtaatt taaaatccga
    17521 agacttgtat tgtttgttca cacgatctaa gtttaacaat gttgtcatat ccatgctcct
    17581 ctttgtttaa ttttaataaa aacgtcttct cttcaataaa taactaaagg cgttatatat
    17641 gaaaatagca atgataagta gcaaactaaa gctttgatta attcctgtag caattgaata
    17701 agaccctata aaaataatgg ctagtatcaa tatggataag ttgattgcat atgttttaaa
    17761 taatgcaatt aattctatat ggtgttcacc ttcatccaat atttccaatc gcttttcagt
    17821 gtaattctta tctgcaattt ttggcattct atcatcaaat cttctattaa acagcgtaaa
    17881 ttgtgtattg aaaatagcac tagcaaaaaa tggtattaaa aataatagta ttccatttac
    17941 ttcagcttgt cctaccacaa aaataagcaa cactaacaag gcaataactg tctgtagaat
    18001 acttaatata cttccattta aaacatgacg attcgcaagc aattcatatt gatctgtttc
    18061 ttcgttatct acctcttcat ctacaagtcg cttatacttc aatgcacgtc tttgattcat
    18121 caaaacaatg atttctatta atatgatgat tatagtcgca ataattgata ttacaacgac
    18181 attgttatgc gttgcgaacg tcaaattctc aatacccaat ccatcaaagt tacctaaaat
    18241 tccacctatg ataccaccta caagtccacc tagtaaaaga tatccgatat atcttagtat
    18301 tttcaaacct catcctcctc aataataaaa atagtttcca ccttttcatt gaagatacga
    18361 gcaatcttta ttgcagttaa taccgatggc ataaagttgt ttcgctcaat tagcgaaata
    18421 gtttgtcttg aaacacccgc ttgtttagca agctgcgttt ggtttaagcc atctcgtgct
    18481 cgtaattctt tcaatcgatt acgcacatcg catcaactcc tcaactacac tgtaaatgat
    18541 aattgtcttt ttgacaacta tatttgtcaa atctacacaa aaaatataag tgtccaccta
    18601 tgtatgacat tttgaaacac acacctcaac acctacaagt cataattgtt tactttcgtt
    18661 acaccttcct gcataattaa cagcattcta attttagtat gatgcacgca ttttcactaa
    18721 atcaaaccat tcaaaggaga ttattatggc atttaaatta tctgcaattc aacaagcaca
    18781 tcaacaattt actggtgttg attttccaaa actattcaag gcttttaaag atatggggat
    18841 gacttacaat atcgtcaata ttcaagatgg cacagcaaca tacgtacatc aatcagaaga
    18901 tgatatcgtt acgtcatctg taaaaagtaa tcatcctgtc gctccaagtt caaatcaatc
    18961 tatcgttcaa gatgtattaa ctagacatca acaaggacaa acagatttcg aaacattttg
    19021 tgatgaaatg gctcaagctg gcatttataa atggcatatc gatattcaag ctggtacttg
    19081 tacttatatc gacttgcaag aacaagctat tatttcagaa ttaatacctc agtaagttgt
    19141 gtttatagca atattttaat tattatataa aatttgttga taatcattat cgttcggtat
    19201 aaagtaaata ctatatacta cttatgagtg aggttgatta tcatgataac taacactttt
    19261 attttaggca tcacaggtcc aacaagtctt gtcgtcatta gcattatcgc tttaattatt
    19321 tttggtccga aaaaattacc acaatttggt cgtgctatcg gttctacttt aaaagaattt
    19381 aaatctgcta cagaagattt agataaagag tctcacgata cacccagtaa ggaatcgaag
    19441 caacagcgag agcaatagca ctgatgatac attaatggtt cactttagcg aactacgcca
    19501 acggttagta aaaattttat tgtcgttcgt cattgcggtc atcgtcgtat atgtttcatc
    19561 tttttggtgg atgacaccat tcataacgta tattacccgt gcacatgtgt ccttacatgc
    19621 attttcattc acagaaatga tccaaatata cgtgatgatt atatttttca tcgcattttg
    19681 tttcatttca ccagtcatgt tttatcaatt atgggcattc attgcacctg gattacataa
    19741 taatgaacgt caatttattt ataaatatag ttttttcagc gtgttattat tctgtgctgg
    19801 cgtcgctttt gcattttatg ttggctttcc aatgatcatt caattcgcgt taaaattatc
    19861 attaacttta aacatttcgc ctgttattgg ctttaaagca tatttgattg aactcatacg
    19921 ttggttattt acatttggca ttctatttca attaccaata ttatttatgg gtcttgcaaa
    19981 attcgatctt atagatacat attctttgaa acattatcgt aaatatattt attttgcttg
    20041 ctttgtgctg gcgagtatca ttgcaccacc agacataaca ttaaatatat tactcacatt
    20101 gccactcata ttgctctttg agttcagtat gttcatcgtt aaattcacca gtcgaaatga
    20161 cttatcgtcg caatgacctt atacgaatca atactgcaat actcacaatg caacaacatc
    20221 acaatagcca ataaaagaac aatgtagcac ctcagattat atcaatactg cagctaacta
    20281 acattgcgtt aatcagaata cagcatcgga ttacaccgtg atatccgcaa tgcaacgaga
    20341 accaacaatg cttcgatatc tgcaatacaa cgagaaccaa caatgcagtg atatccgcac
    20401 ctcaacaatg ccgcgatatc cacaatgcta agataatccg cacctcaaca atgcggcgat
    20461 atctgcaatg cttcgatatc cacaatacag caacaaaaaa aacaggcact cggcctgttt
    20521 tttatttttt aaatttaaag attaagatag caactaccat aatataggca gcttgtgcta
    20581 tcaatggttc gtaacttgga tagaatccta accagttaat cgttgggaat ccttcaataa
    20641 catgtcttgg catcgcacct aataattgta acttttgaat acttacgcca agcattttga
    20701 atcccataat aaaaataaag atcgacaata ctctaaatat atagaatata ggtatcaatc
    20761 tgacgataaa tctaaataat agcgcaaaga caattaatat cacgatggcc aaggcaatac
    20821 ctatgacaaa atctttggtc gctaattcgc ctatcatccc catatagaaa atgataacct
    20881 caacaccttc acgcaacaca gatattaaac caatcgtcgc taacaatacc aaattaccat
    20941 tactaatcgc attggtatac atatttttaa tcatatcatt ccagcgtttc gcattcgaac
    21001 gtttatgcat ccaaacacca acgataaaca ttaatatgac cgcaacgata cctaatcccg
    21061 cttccatact ctcacgaagc ataccgctat tccctagtgt ttctacgaat gtaattgcta
    21121 aaacgatact caatacaagt ccggcaattg caccaccaat cacacttgca gtacccttct
    21181 tatctttgac attacgcgtc atagtagtca atgtcattac aattaataac acttctagtc
    21241 cttcacgtaa aaagataatc atcacatcga cgaagctata actatggcca acaacctctt
    21301 taatttgatt gtttatatct actaaaccat ctttgacatg tgctttatta tgttcgtcta
    21361 atacactttg ataatatggt attttatctt caattttcgt atacaaagca ccgtctttag
    21421 tttgaatttg accttcaaca tatggccaaa tttctataaa atgtgtaagc gcagcatcag
    21481 catccgacaa ttgattgttg tcgatagctt taatcgcctt ctctaactca tcatttaatt
    21541 gtgatacatg gtattgatca tttgcagacg taccactttt cttatcgaca tgatcaatat
    21601 ttgatttaaa agttgtccaa gcatgtgaca cttttgccgt atctaatggt gacttatgaa
    21661 ttgcaattct aagttgtaat aatgcgactt caatttgtcc atattgattt gcgtcataat
    21721 tgcgaatcac tgtttcatta ctagtccaaa tctgatttaa actattgttc aaagattcta
    21781 attccgcttt atttttgtct ttaatcgctt ttgtcatcgc tgcatctttg gcatcgactt
    21841 gctgttgcaa cagtttaatt ttagaacccg catctttact agccaatttc tcttcataag
    21901 caattaatga cttcgttaat tgcgaaagtg tatctttttg atcatcattc gcttttgcat
    21961 cttcaagctt tctcacattt gatttgacag cattaccttc gctattatct tcaagcgata
    22021 atttcttaac tgcacttacc acttgctcaa ttgctttcag cttattgtct ttcgatatcg
    22081 aattattaga aagtgcagat tttgcatccg ttatcacact atatacatca ctaatacttt
    22141 gttgttctgc tgcctgactt ttcaataacc caaagctaaa taccataact aaagtaatta
    22201 tcgtcgctat aattttagtc aaataatgtt tcaccaaggt atcctccctt actaacacct
    22261 ggtaatacta aaaatgaagc agaacctcta tgtgtaatgt attcatttaa cttatcatta
    22321 ctacctaaat tgttttgaat atcgataaat tgttgggtcg ctttttgaaa agcaatgaac
    22381 aacaagcctg tttcgaagtt acctgtgcgg tcatccgtac catcaacata attaaaggct
    22441 ctacgtaaaa ttgacgtatt tgcttctttc gctagtctcg tatgggcatc tttatcaata
    22501 atatactcgc catgactatc tttcgctttt aagtcaattt catcaaactc tttcccacct
    22561 gttaacggtg caccactatg tcgcttgcga ccaaatgtag cctcttgttc ttccagtgca
    22621 gtacgatccc acgtttcaat gtgtatttga atacgtctga caacacaata agttccatgt
    22681 ttcgcccatc cgtcatcaat aaacacataa tctttaagtt gattactctt tctaggatta
    22741 attgttccat ctttaaatgc cattaaattt ctaggtgttt ccttaccttt agcagagata
    22801 aaaccagatt gcgcccaacg taccttaaca atatctcgaa atggacgaac taaattatga
    22861 accgcatgaa aggatacttg cgaatcattt gagcatgctt gaatcataat atcaccatcg
    22921 ctgtaatcgt ctattaactg atcattcgga aaatgcggta aatctttaaa ggcatcggga
    22981 attttactag ataacccaat tttcttcatc aaagacttac taattccaaa ggtaatcgtt
    23041 aacttgcttg cacctaatcc aatcgattca ccagtatcta ctggcggcat taaaggattg
    23101 ttactcggct tgccaactgt atcaccatcc atcatacgat cagccataac cgtccacttt
    23161 ttaaacattg ccttaattgc atctctatct tttgacttca aatctaatgc aacgaaattg
    23221 catgtttttt gcgtgggtgt agtaatgcct gattgcactt taccataaaa ttcatacgca
    23281 tctttttccg gatcttctgg tgtattgaac attgatttga aagaccacat gctaccaaca
    23341 ccacttgcgc caattgcaac accggcaccg ccaataccta acattttcaa aaatgtacgt
    23401 cttgaaaatt gcgtactatc gttaacttgt tcataatttg tcattcagta atcacagcca
    23461 ttttacttag tggctctcct aatgcattaa cagcatccgc aaatgcttta cggtctttct
    23521 tcgatacttt ttcaaatgac tcataaccgc cgttgttatc tttatactta tccaataatt
    23581 tattcacttt atcgaagttc atttgaatat catcacttaa ttttttatct tttttctcta
    23641 aaataggttt aaataagtca taaatttttt gtgcgccttc aacgttcgct ttaaaatcat
    23701 ataaatctgt atgtgaataa atttcttctt cacctgtgat tttagaagtt gcaacttcat
    23761 ttaataggtc aacagaacct tgtaacatta attttggtgt gatatctaat gtatcagctt
    23821 tggcatgcaa ttcttttgca tctttcaata attgttgtgc gtctttttta gtcacatcat
    23881 caattttctt atcttcgtat aatgcttttt caatcttatg atatcctgac cattcttttt
    23941 cctttttctc ttctttcata tctgcaagac gtgcatcaat tttaggatct aaatctccaa
    24001 atgcttctgc aactggttca gagcgttcat aatacatacg aacttttgga tataacgctt
    24061 ttgcttgggc catatcatta ttttcaatcg ctttaacaaa tttttctgta ccttttaaaa
    24121 attcatttaa ctgttcatca gtatatttat catatgcttt tgttgcttgt tttaattctg
    24181 ctttaacacc atcatctttt ttcgatgtct ttgaatcatc cttcttacta tcgtcgttcc
    24241 cacatgcagc aattaataac gttgatgcta ataatagcgt tgttaacttt ttcattttga
    24301 cacctcatta tagaaattct attatcccca tagacatgct ctcattcgta ctatctcaca
    24361 ctccctaaat gccaagataa tacgtggctc ttaaaatgat gattttaagg catcctccca
    24421 tgaacattat ctagttgata ataattatca aaatcattat attgttataa aataatactg
    24481 tcaacaacct ttttagtttg aaaataaata aaagagtaac acttatatgt aagtcatcac
    24541 tcttttgtag tctgatttat catccttcat tatattctga ttttaataaa ctatagatgt
    24601 aactcgatga ataaatacca tttagtaatt cattgtcacg taacatacct tccctagtaa
    24661 atcccagcct ttcaggaata gcttggcttt tttcattatt aactgcggca ctgatctcaa
    24721 ttcgatttaa ttcaatctca tcgaagcaat actttatcaa tgcctcaact gcttgtgtca
    24781 taatcccatg accctcaaat tgtttatcta aataataccc taatgaagtt tttctgtgca
    24841 tgtgattaat ttcatgtaaa ccgatgacac caactagcgt tccttcatac caaatgccac
    24901 actgaaatcc attaccatca gcaaattgca aaagtccact tttaataaat gcacgcgtat
    24961 ctgatggttg ctcggttgca tctacccaag gtaaccattc cctaagtgaa tttcttgaac
    25021 gattgactaa attgaaaagc gcttctgtgt catgggcttc taaaattttt aatcttattt
    25081 gttcattcac tttcattcca aacataccat cacaccctca tttatttttc atataattct
    25141 gtaaattatc ataataataa catattaata ttacgccttg tatcttaaat ttgttgtcct
    25201 tccccaactt gcattgtttg tagaatttct tttcgaaatt ctctatgttg gggccccgcc
    25261 aacttgcact gtttgtagaa tttcttttcg aaattctcta tgttggggcc tcgacaataa
    25321 gcattgtagt gcctattact ttggttgatg cccttgatac ctatcacaca acttaattta
    25381 atcactactt tattaaccat tccaaattta tatttaaaaa tacgacaaaa cagtgctaaa
    25441 atgctattaa aggaggcact gctcatggca tcattgaaaa ataagcattt tatgattgga
    25501 ctatcattaa catttatagt cgcattattt agctttttag cagctaaatt gccaatctta
    25561 gataaggttg gcgcattaac tatcgcgata ttaatcgcta ttctgtatcg tcactttaaa
    25621 ggatatcctg aacaatatag ctcaggtatt acattttcat ccaaatattt attaagattt
    25681 gcaatcatcc tatatggact taaactcaat atattcgata ttatcggtca aggtagtaga
    25741 ctattagcca tcgatgtagg tgtcgttatt ttcagtattg taatgatgct ctttgtcaac
    25801 aaactgttgc gtggtgacaa aaacatcgca ttattactag gtgtaggtac aggcgtttgt
    25861 ggtgctgccg ctattgctgc agtagctcca atattcaaat cacgtgaaaa agatacagct
    25921 attagtatcg gtatcatcgc attgattggt acgatatttt cacttatata tacagctatc
    25981 tatgctatct tttcaatgac gacaaatgtt tatggcgctt ggtctggcgt tagtcttcat
    26041 gaaattgcac acgttgtctt agctggtggc tttggtggta gtgatgcact taaaatcgca
    26101 cttcttggta aacttggtag agtattctta ctgattccat taaccatcgt acttatttta
    26161 attatgcgct tccgttcatc agaatcatct agcaagggtc gaataagcat tccatacttt
    26221 ttaattggat ttgtcattat ggccttagtg aatacatatg taactatccc gtcagtattg
    26281 ttaaatattt taaatactgt atcaacaatc tgcttattaa tggccatggt tgctttagga
    26341 ttaaatgttg catttaaaga tcttaaaaat cgtgcactta aaccgcttat gacaatcatt
    26401 attacctcaa tatgcttgtc atcacttgcc tttatcgtcg ttcactggct atatagttaa
    26461 tatgaaaaga cgctcaaaca cttacaaatt tagtgtgccg agcgtcttat ttttatactt
    26521 taaaagtcgt attggttatt ttcttcttca agcacttgtt gttgtacttt ggcagttcgc
    26581 attgtggatt gtgccaacgc ttttaaacga aatacaacat catcattgac aatttgttga
    26641 tttgaaaagt ctttctcttc aatgaataca tacgtttgca tcgtatgtgc tttcatgtaa
    26701 ctcaatattg gttttaaatg catttcagga attaaataat gtttactaga acctgctgtc
    26761 gctacaagtc ctattacctt atcatgaaac gcattgactg gaagtaaatc aaacacattt
    26821 ttcaaagaac cagggatgga agcttgaaaa attggaaaac caataaaaat cacatcagcc
    26881 tgcattaacg acgtcgttaa tttatataca tctcctgtag tatctagata attctttcca
    26941 acactaaatt ataattcgag atctcgtaaa tccatcaatg ctatttggtg tccctcattg
    27001 atgacttcta gttcattttt taaatcatcc atagcaattc tcgttttaga acctactgtg
    27061 gagcctgaca ataatacaat attcatgatg tcgcccctcc tattttgata aatgcttttt
    27121 aatcgctggt ataatgtcgt tgccaattaa ctcaatattc ttcataacat tttcaaatgg
    27181 cacaccgcca aaatcaagct gtgccataaa acgttgatga ccatataact cgtgttgata
    27241 caatatcttt tcaataattt gttgcgggct tccaaccatt agcgcttctc gataatctga
    27301 cgaattagca aattgctgtt tcggataacc aacgccacga ataaatgaca tccctgtatt
    27361 caaatgtgga taaaattctc tcatagcatc ctgagttgtt ttagctgtat aaaacagact
    27421 cgctgtactt actggtaaag acttaggcga agcatcgaaa cctgcttcag ttgctgcttg
    27481 acgataagca tctatagaac ctttaaagtt cattgctggg ccaccaaggg ttgtaatcat
    27541 cattggcaca ccttgttttc ccgctttgat agcacttgca ggtggaccac caacagcacg
    27601 ccatattggc aatgtattat cgattggtct tgggaatatt ttcatatttc taagttctgg
    27661 acgatatttt ccagaccaag taatacgctc agttttattt aactctaaaa gtaaacctaa
    27721 tttttcttca aacaattcat catagtcttt taaatcatag ccaaacaagt caaaaacacc
    27781 tgttcttgat gctctgccag ctacaatttc ggctctacca tgagaaatca aatctaatgt
    27841 cgcgaagtct tcaaatactc ttacaggatc tgttgcacta ataatcgttg aagaacttga
    27901 aactttaata tgctttgtcg cttgggcaat tgcacctaac acaaccgtat gtgcctgtgt
    27961 cgtaaaatgt tcctgatgac tttcaccaac tgcaaaaaca tcaatacccg cttcatctgc
    28021 taatttactt gcttcaataa tttcattaat acgttgctca taactaactt tttcaccttt
    28081 aaatggattc aataaatgat cacctaagga atacaaacca aactcaagag gcgtattttt
    28141 attcatttct aattttgcca ttaaacattc tcctttatat ttcgtgtaac ttactttcaa
    28201 tttgttcacg ttctgcttct aaaaagtcag gcaagttcaa ttgacttcct aattgttcaa
    28261 caggtgtatc aatagtaaat ccaggcgctt cagtcgcaaa ttcatacata attgaattat
    28321 ggcgatagta taatgattta aagggtggtg taaactctag ttaacagtta attcttatga
    28381 aaggaattga taattatgac tgagtttaca cctttttttg atggttcaaa tcaattaaat
    28441 tatcttaaat ttgtaatttt aagatttcat ggtgtttcta aaagaaaaat ttgtaaaatg
    28501 tataattttg catattttcg tatattagaa gtttgtgaaa tggcaaaaaa gaatgattat
    28561 cgatttactt ataaggatta taaattttta aaacaatatg gtgtgtctaa tacatttatt
    28621 tgtaagatgt atcatatttc aattgaagac ttagaatttt ttgaggtgat gaaccgatag
    28681 gtttgattaa aaatatgata ggtcaggaga agcatagaca ctccattatc aaaaatgcag
    28741 tggtacggat ttgcgggagt tattcctatt cgtgcgaccc tttagaagtc gtttaatttt
    28801 attaaaaatt ttgtaaagga tggatatatt atgaaaccag tattggatat taaaaaaact
    28861 ttaggacaat taaacttttt aggtgtagat gaaaaatata aatatgaaaa tggagagcgt
    28921 actgataaaa ctgtttatgc atataaatta gcgagtcaag aacaaggcga acaaatcact
    28981 gttaaaacgc ctaataaagt ggaattagac tatttacaag aatgcgaatt agttgaacca
    29041 gacgttaaaa tgtatgttca aatgtcaggc gattttggaa ctatcgcata tagctggaat
    29101 gcagaagata ttaaagtagt tggttctaat agtgcactta aacaaaaata attaggtgat
    29161 gtaaatggat tattatacag cagatagatt atatcgctac acaaatagtt caaatttgag
    29221 tgaacctatt ttaaactatg ttgctagtcg tataaattgg ggcgataaag tttcattaat
    29281 gactttagct aaagaaatac agtctaaatt taatgatagt tacgttaaag aaaatacggt
    29341 aaaaggtcgg cctaaaattt acgctgacct ttgtctattg tgtatgagct tgagtgaagc
    29401 aggacatggc agaatgctac aagttaattt ggaagattgt atatatattg gcgatattga
    29461 tgtttaacat atagtattag tgatttaaca tgaccgtgta gcttttagcg tatgcaggcg
    29521 cagaaatgca agcacggagt gcgttaagca tttgggcgga gcctgcgaca aatacgcgtt
    29581 aaaaaagcga gaacggtcat gtatcaaatc gcttgcgatt tgataggagg cataaataaa
    29641 tatgacttta aaaaaccagt gta
//
LOCUS       DAKSQE010000021        28054 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.021, whole genome shotgun sequence.
ACCESSION   DAKSQE010000021 DAKSQE010000000
VERSION     DAKSQE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 28054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28054
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.021"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(185..1411)
                     /gene="ftsW"
                     /locus_tag="O0V33_000804"
     CDS             complement(185..1411)
                     /gene="ftsW"
                     /locus_tag="O0V33_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCX8975468.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKL
                     ILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLV
                     VFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAM
                     KLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVG
                     IATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQ
                     QRKKQQQKVDIRRQFN"
     gene            complement(1725..2000)
                     /locus_tag="O0V33_000805"
     CDS             complement(1725..2000)
                     /locus_tag="O0V33_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCX8975469.1"
                     /translation="MAKQTTMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            2141..2623
                     /locus_tag="O0V33_000806"
     CDS             2141..2623
                     /locus_tag="O0V33_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975470.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            2625..2792
                     /locus_tag="O0V33_000807"
     CDS             2625..2792
                     /locus_tag="O0V33_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HCX8975471.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(2931..4778)
                     /gene="typA"
                     /locus_tag="O0V33_000808"
     CDS             complement(2931..4778)
                     /gene="typA"
                     /locus_tag="O0V33_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCX8975472.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTLINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            4880..5071
                     /locus_tag="O0V33_000809"
     CDS             4880..5071
                     /locus_tag="O0V33_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HCX8975473.1"
                     /translation="MKQKKSKNIFWVFSILAVIFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(5225..6052)
                     /locus_tag="O0V33_000810"
     CDS             complement(5225..6052)
                     /locus_tag="O0V33_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HCX8975474.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWVVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPVLGEIFKDVVNISRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILNE
                     VDGLASNLLGEPLTISGPNSILVGNRGVHREILGVYLEPHRDALIQLHEQRFKRKSK"
     gene            6209..6823
                     /locus_tag="O0V33_000811"
     CDS             6209..6823
                     /locus_tag="O0V33_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HCX8975475.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLHELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATSKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERKFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLKEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
     gene            7007..8359
                     /locus_tag="O0V33_000812"
     CDS             7007..8359
                     /locus_tag="O0V33_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCX8975476.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIVQTFQELQG
                     "
     gene            complement(8441..8860)
                     /locus_tag="O0V33_000813"
     CDS             complement(8441..8860)
                     /locus_tag="O0V33_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCX8975477.1"
                     /translation="MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAK
                     QLSQNGGKVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSL
                     MALNLIAIVLWIIVMIMLISKKESKETTHKDDEYIYH"
     gene            complement(9143..10162)
                     /locus_tag="O0V33_000814"
     CDS             complement(9143..10162)
                     /locus_tag="O0V33_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCX8975478.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQELERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
     gene            complement(10236..11309)
                     /locus_tag="O0V33_000815"
     CDS             complement(10236..11309)
                     /locus_tag="O0V33_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HCX8975479.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     LLPIDHNKVPNIKNLDSDYMNMSFDKGNKYSLPYFFGTVGILYNKEKYPNESFDSWKS
                     LYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVG
                     DEITMMLEQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKE
                     GAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVY
                     KDLGPEVLSEYNENFLNFKMSLK"
     gene            complement(11309..12118)
                     /locus_tag="O0V33_000816"
     CDS             complement(11309..12118)
                     /locus_tag="O0V33_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8975480.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            complement(12124..12921)
                     /locus_tag="O0V33_000817"
     CDS             complement(12124..12921)
                     /locus_tag="O0V33_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8975481.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYAALITIITLAISYPAAYYITRSKFQNILLMIMIIPTWINL
                     LLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
                     KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGN
                     KVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
     gene            complement(12914..14008)
                     /locus_tag="O0V33_000818"
     CDS             complement(12914..14008)
                     /locus_tag="O0V33_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8975482.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            complement(14021..14560)
                     /locus_tag="O0V33_000819"
     CDS             complement(14021..14560)
                     /locus_tag="O0V33_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCX8975483.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEVYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            complement(14704..14979)
                     /locus_tag="O0V33_000820"
     CDS             complement(14704..14979)
                     /locus_tag="O0V33_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCX8975484.1"
                     /translation="MEYEYPIDLDWSNEEMILVINFFNHVEKYYESGVTAGDFMDAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
     gene            complement(15150..16556)
                     /gene="lpdA"
                     /locus_tag="O0V33_000821"
     CDS             complement(15150..16556)
                     /gene="lpdA"
                     /locus_tag="O0V33_000821"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCX8975485.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNAIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            complement(16560..17852)
                     /locus_tag="O0V33_000822"
     CDS             complement(16560..17852)
                     /locus_tag="O0V33_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCX8975486.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASATNEEVAETPAAPAAVSLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            complement(17943..18920)
                     /locus_tag="O0V33_000823"
     CDS             complement(17943..18920)
                     /locus_tag="O0V33_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCX8975487.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDIDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            complement(18924..20036)
                     /gene="pdhA"
                     /locus_tag="O0V33_000824"
     CDS             complement(18924..20036)
                     /gene="pdhA"
                     /locus_tag="O0V33_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCX8975488.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQTAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            complement(20207..20833)
                     /locus_tag="O0V33_000825"
     CDS             complement(20207..20833)
                     /locus_tag="O0V33_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HCX8975489.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            21198..21749
                     /locus_tag="O0V33_000826"
     CDS             21198..21749
                     /locus_tag="O0V33_000826"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCX8975490.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKDHPLQPHTDAVEV"
     gene            22080..22202
                     /locus_tag="O0V33_000827"
     CDS             22080..22202
                     /locus_tag="O0V33_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975491.1"
                     /translation="MNDMMNLVINQIVIKLHYRDDWNEFIDIYSNTILLGLTIN"
     gene            22231..22449
                     /locus_tag="O0V33_000828"
     CDS             22231..22449
                     /locus_tag="O0V33_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCX8975492.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            22449..24146
                     /locus_tag="O0V33_000829"
     CDS             22449..24146
                     /locus_tag="O0V33_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCX8975493.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
     gene            complement(24494..25156)
                     /locus_tag="O0V33_000830"
     CDS             complement(24494..25156)
                     /locus_tag="O0V33_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCX8975494.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
     gene            complement(25289..26308)
                     /locus_tag="O0V33_000831"
     CDS             complement(25289..26308)
                     /locus_tag="O0V33_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCX8975495.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFAISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYV
                     EGKKS"
     gene            complement(26305..27666)
                     /locus_tag="O0V33_000832"
     CDS             complement(26305..27666)
                     /locus_tag="O0V33_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCX8975496.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSLLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRNFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYTILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            27864..>28054
                     /locus_tag="O0V33_000833"
     CDS             27864..>28054
                     /locus_tag="O0V33_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HCX8975497.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNERQIDFEERNIKNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHV"
BASE COUNT         9261 a         5080 c         4048 g         9665 t
ORIGIN      
        1 taaaatttga taatcatgag taatctcgta atatcaaaac aaaaaggaag ttaagcgttg
       61 tttggttgct taacttccgt tattgaactc atcagtccaa tttgacaaag agtatataaa
      121 tagccaatgt gtagcattgc ttaatgacag aagtcatctc gcgctaaata ttggctagta
      181 ttttttaatt aaattgtctt cttatatcaa ctttttgttg ttgtttcttt cgttgctggt
      241 ctactttgat ttgtttacct acaatcagaa gtaatcccat agcaatactt aaactaatca
      301 ttgatgatcc accaaagctg ataaatggca atggcacacc agttaatgga attgttgccg
      361 aaataccacc aatgtttaca aacgtttggc ttccaaagta tgtggcaatc ccaacacaca
      421 caagtttata aaaatatgat gatgttttat ttgcaaactg gaaggcacga tatacaataa
      481 agaactctaa agtaataact agcaatcctc cgattaaacc taattcttcg caaataattg
      541 caaaaataaa atctgtatgt ggttctggta aatagcccaa tttcattgca ctatttccta
      601 atccttttcc aaatacgcca ccgttaccta tcgcaagcaa tgaattggaa atatggtatc
      661 cagttcctga ttcgaattgg aatggatctg ttagcgtact aaatctggca gttaaataac
      721 ttggtaacca accagccatt aatgcaatga caaatactac taagaatcct agtactgctg
      781 gtattccaaa tcttaggact ttgtttaccc caattcctga ataaaaaata atcgcaacta
      841 aaataattaa tattagtaat gtttgcccta cgtctttttg taagaaaact aaaaacgtac
      901 aacctaatgc taatacaata ggacttaaaa ttaattttgg tttacttaat actctaggca
      961 tttttttact aatcataaat gggatatata atatgattgc aatttttaat aactcagatg
     1021 cctgtaagtt cataaatcct aagtttatcc aacttttaga accattaata tctttaccga
     1081 ttactaacgt cagtaataat agcgagacga tagttataat catacctttt tgcactttaa
     1141 tattactcag taatttaaca ttcattaaaa atgcaataaa aaatacaatt ataaaactca
     1201 ttatgacata tgctaattgt cggttgtaaa aatacgttcc tggaacatcg ataccaccag
     1261 tcaatgtacc tttagttgct ggaaccatac tggcactata taccatcact aatccaatta
     1321 aactcaatac aatatatgta actaataacg ggtaatcaat aaactttgag gttttaccaa
     1381 tataccgtaa aatacttcta aaattcttca tatattcagt catccaattc tacaatttat
     1441 acttcaaatg atttcgtttt atactttaat tttaaaaatc catgttactt attatataca
     1501 tgacacagtc ataatcacaa atattaacag gtctcttgag gtaatttata gaatatttgt
     1561 ttacttaata tcgtgaattg ctaaaaccca ataaaagtga ctgacatgtc atcaagaaga
     1621 caacataaca gccactttaa tcaataatta aattataaat ataaaatcgg cgctgctcaa
     1681 ttgtcgatat ctcacgccgt tttaatatgc ttatatatta aaccttaaac aagtgtaaaa
     1741 gcttcatgta attttgaaag ttctttctca agacgtaaca taatttgttt gccatcttcg
     1801 cggtcaacta aacctaattt aacagcaaaa tcaacttctt tttgaagtcc aaacatttgt
     1861 gtatctagta cttcttcata taatgggcat gaaggtaatg ttaaattatc tagttgaact
     1921 ttaatcagat gcaagatttc atcagcatct ttagtcaatt gtttcaaagc tgcatttttc
     1981 attgttgttt gtttcgccat gacacgctcc ccctctatat aaattctcaa gtcttactaa
     2041 aagtttatct tactaaatat taaaaagcaa gtagatttaa aatgtgtcgt tagcaatttg
     2101 tgttaaaata atgctatata cattgatagg gagtgggaaa atgatacaaa ttaaaggagc
     2161 tgtaaagttt cctataacat tagatagtac gacttggatt ttcgatgata gaaaagttag
     2221 catcgaagat ttagaaaaag gtgtatttga tggttcaaaa ccaatcgact ttgaagataa
     2281 tcatgaatgg aatcgtgcta tcctagaagg acaaactaat ccgcctactt taaattcaga
     2341 aataaaatat aaaaaacgtg ccatgttaga aggttcattt gtcattaata tgacaccatt
     2401 tttcaaacat tccgaaccac atgactctgc aactactatt cgattatcaa atgagcatga
     2461 tcatttagat ataccgatag atctattgcc ctatttattt ttccaatttg caaaagacgg
     2521 taaacgactt tacagcgata atggtgtaga tagttttatt tacaatcaag aagatggtta
     2581 ttcatatcga atcaaccatg taactcatat tgaggtgatt taatttgaga caagtgcaat
     2641 gcattatctg tgatgcaaaa gtatttatag atgagcgcac aactgagtct aaacgactta
     2701 aaaacaatcc tattcgaaca tttatgtgcg atgattgtaa aagtcgacta gacaccccta
     2761 aacaacgtgc gcaacactat ccgctcgatt aatatctgaa ttaacaagtt agaaatttga
     2821 aaatgtgaaa gtaacaggaa ctgatagcat taaaacactt tttcaaatat tatatgccaa
     2881 aagagaagct aagcaacttt catcgctcag cttctctttt attatgttaa ttattcattt
     2941 tcttgcatca tttgtttgat acgctttgct tctttttcac gaacattttt gtttaaaatt
     3001 ttctttctta aacgtatgct ttctggagta acctcaacaa gttcatcatc attaatgaat
     3061 tgtaacgctt cttccaatgt tagtatacgc ggtctattca ttgtttgtgt ttggtctttc
     3121 gttgcagaac gtacgttagt ttgatgtttt gttttagtga tattaacagt taaatcattt
     3181 tcacgattat gttcaccaac aatcatacct tcataaactt cagtaccagg ttccatgaag
     3241 tttacacctc tatcttcaag tcctaaaatg gcataagtac ttgcagaacc ttgatccatt
     3301 gaaattaacg caccattacg acgaccgcca atttgcgctt taatacgtgg tctaaattct
     3361 tcaaatgtat ggttaataat accgtaacct cttgtcattg acataaattc agtcgtataa
     3421 ccaatcatac cacgagccgg tacattaaag attaaacgtg taagtccatt atcagttgta
     3481 gtcatatcaa ccatttcacc tttacgtgca cctaatgatt caataaccgc acctgcattt
     3541 tcttgtggca cttcacattg cacacgttca aatggttcac acattacacc atcaatttct
     3601 ttaataatta cttgtggttt tgaaacttgt aattcataac cttcacgacg catattttca
     3661 ataaggattg ataaatgcaa ttcacctcga ccagcaacta cccatgtatc tggagaatct
     3721 gtgttagaaa ctttcaaaga tacatctgtt tctaattgtt gatttaaacg ttcttgaatt
     3781 tgacgcgctg ttacaaagtc accttcacgg ccagcaaatg gtgaattgtt aactttaaat
     3841 gtcatttcaa gagtaggctc atcaatacgt agaactggca atgcttcttg atggtcatgt
     3901 ggtgttacag tttcaccaac attaatgtct tccatacctg aaacagcaat taaatctcca
     3961 gcttgtgctt cttcaatttc taaacgtttt aatccaaagt aaccaaagat tttagttaca
     4021 cggaagtttt tcactgtacc gtctaattta attagtgata cattatctcc gacacgcatt
     4081 ttacctctga atacacgacc aataccaata cgaccaacat aatcattgta gtccaacaat
     4141 gctacttgga attgtaatgg ctcatcactg ttatcaattg gagctggtac ataatcaata
     4201 attgtttcat ataatgattg taaattatca tcttgttttt caggatctaa gctagctgta
     4261 ccatttactg ctgaagcata aacaacaggg aattctaatt gttcatcgtt tgcttctaat
     4321 tcaataaata aatctaaaac ttcatctaca acaccctctg gacgtgctga tggtttatca
     4381 attttattaa caacaacaac aggttttagg ttttgttcta gcgctttttt aagtacaaaa
     4441 cgtgtttgag gcattgtacc ttcatacgca tctactacta agacaacccc atcaaccatt
     4501 ttcataatac gttctacttc tccaccaaag tcagcatgtc ctggtgtatc caaaatatta
     4561 ataagtgtac ctttataatc aacagccgta tttttggcta gaatcgtaat tccacgctct
     4621 ctttcgatat cgttagagtc cattgcacgt tcatcgacat gttcattttc tctgaatata
     4681 ccagattgtt ttaacaactc atctactaaa gttgttttac catggtcaac gtgagcaata
     4741 attgctatat tgcggacatc ttctctttta ttagtcatat aaatagtttc cttctttctt
     4801 aagtaaaatc ccacttttaa ttacaacttt tttattatat catataattg agaaaagata
     4861 agcaaaaggt ggggatagga tgaaacaaaa aaaatctaaa aatatctttt gggtattttc
     4921 tatattagct gttattttct tagtattatt tagttttgct gttggtgcat caaatgtacc
     4981 aatgatgatt ttaacattta tattactcgt tgcaaccttt ggaattggat ttactacaaa
     5041 gaaaaaatat cgagaaaacg attggctata agttatatcg acaataacac gcgttgatta
     5101 aagaccacac atgttcatga tgaacattta aatggtttta tattggcgcg tgtttttatt
     5161 atttaagata taaaaacgta atatgtcacc taattgttag gactacatat tacgcttttg
     5221 gtaattattt tgattttctt ttaaatcgtt gttcatgtaa ttgtattaac gcatcgcgat
     5281 gtggctctaa atatacaccg aggatctctc tatgaacacc acgattacca actaagattg
     5341 aatttggacc actaattgtt aatggttctc ctagtaaatt agaagctaga ccgtccacct
     5401 catttaaaat gactagtcct ccagcaaaat cccacggttg gagtctaggt gtcatatatg
     5461 cttctaaatt accggtagcg acagaaacaa tttcaagcgc tgcactacca tatgcccttg
     5521 cacttcttga aatatttacg acatccttaa aaatctctcc tagcacaggt ttagtcaacc
     5581 agttcggatt gatcccaata atgctttgtc ttagattaga atcattcaat ggtttcaagg
     5641 gttggctacc tcgatatgca ccttccccta ctttagcatg atataagaca tcagccataa
     5701 catcatatac aaaacctgca taaggtttac catcgatata aataccaatt gaaatcgcga
     5761 aattttcttg ttgatgaaca aaattcaatg taccgtctat tgggtcaaca acccataccg
     5821 tacctttgga agtatcgatg tcatgaccat gcccttcttc acctaatact tgatgattgg
     5881 gatatgtttc taaaattgta tcaaaaataa aatcttctgt tgctttatct acatttgtaa
     5941 caaggtcatt cggatttgac tttgtttcaa ttgttaaatt ttgctccatc aattgtttaa
     6001 ttctaattcc tgcttcttga ataagtcctt gggcaaatcc ataaagtgcc ataacatcat
     6061 ctcctaacat ctttattata catcaacatt ttataaaaat aacttcttat gataaaaatg
     6121 aaaatactaa ttttaatgag agcgtcttaa tccgcaattg ataaaacata tgctacaata
     6181 tttttaaaca ctatacagga ggtactctat gacaaaatat acatttaaac ctaaagattt
     6241 caaagcattc aacgtagaag gcttagacgc acgaatggaa gctttaaacg aatacatacg
     6301 accacaactc catgaattag gagaatattt tagtgacttc tttacaagtc aaactggtga
     6361 aacattttat cctcacgtag caaagcatgc tagaagaagt gtgaatcctc ctaaagatac
     6421 atgggttgct tttgctacaa gtaaacgagg ctataaaatg ttacctcatt tccaaattgg
     6481 tatgtttgaa gatcaactgt ttgttatgtt tggaatcatg catgaagcaa aagataaagc
     6541 aacacgtgca aaagtttttg aaagaaaatt taaagctatt caacaattac ctgatgatta
     6601 tcgtgtatgc ttagatcata tgaaacctga taaaccattt attaaagatt taacggatga
     6661 tgatttaaaa gaagcgatac aaagagccat caatgtgaaa aaaggtgaat tctttatagc
     6721 gcgtgcaatc acaccacaag ataaaagatt aaaaagtgac aaagcattta ttgcattttt
     6781 agaagaaacc ttcgatcagt tcttaccatt ttattctgca taaataactt tgtttaaata
     6841 atagagcacg taatcacatc catgatttcg tgctcttttt acttaccatt aaattgaacg
     6901 ttcaacataa taattcatac ttttaaaaaa attaaaataa atttaggttg acctaaacgt
     6961 tttattaggt tattatattg tccataagaa gtagaggtga gtcaaaatga ataataaacg
     7021 acattcaaca aatgaacaat taagtttaga cgaaataaac aatacaatta aattcgatca
     7081 tcgcagttca aataaacaga aatttttatc atttcttggg cctgggttat tagtcgctgt
     7141 tggttacatg gatcccggaa actggataac atcaatgcaa ggtggcgcgc aatatggcta
     7201 tactttgcta ttcgtaattc ttatttcaag tttatcagca atgttacttc aaagcatgac
     7261 agtgagatta ggaatagcaa caggtatgga cttagctcaa atgacacgac attatttatc
     7321 aagacctatt gctataatct tttggattat tgcagaacta gcaattatcg ccacagatat
     7381 tgctgaggtt attggtagtg ctattgctct caatctccta tttaacatac ctttaattgt
     7441 cggtgcacta attactgtac ttgatgtatt tttactacta tttataatga aatatggttt
     7501 tagaaaaatt gaagctattg ttggtacatt aattttcaca gtgttattca tctttatatt
     7561 tgaagtctat atttcatcac cacagttgaa cgctgtgtta aatggattta ttccacatag
     7621 tgaaatcatt acaaataacg gcattctcta tattgcatta ggtattattg gagcaacaat
     7681 tatgcctcat aatttgtact tacattcatc aattgtacaa tctagaacat actcaagaca
     7741 taacaatgaa gaaaaggcgc aagcgattaa atttgctacg atagattcga acattcagtt
     7801 atcaatcgca tttgtagtca attgcttatt attagtgtta ggagcatcac tatttttcaa
     7861 ctcaaacgct gacgatttag gtggtttcta tgatttatat cacgccttaa aaactgaacc
     7921 tgtactaggt gcaacaatgg gtgcaatcat gagtacatta tttgcagttg cattattagc
     7981 gtcaggtcaa aattcaacga ttactggtac tttagcagga caaattgtaa tggaaggatt
     8041 tttaagatta cacataccaa attggttaag acgtttaatt acacgttccc ttgctgtcat
     8101 tcctgttatc gtatgtttaa tcatttttaa aggtaatgca gctaaaattg agcaactact
     8161 tgtattctct caagtatttt taagtattgc tctgccattc tgtttaatac cattacaatt
     8221 agctactagt aataaagact tgatgggacc tttctataat aaaacatggg ttaatatcat
     8281 ttcatggaca cttattatta ttctaagtat tttaaatgtc tatttaatcg ttcaaacatt
     8341 ccaagaacta caaggttaaa aataaaacca ttaaaatgtt ggttgtatct aataattaac
     8401 aacttaacat tttaatggtt tttcttttgc tatgaacata ttaatgataa atatactcat
     8461 cgtccttatg ggtagtttct tttgattctt ttttagaaat caacataatc atcacgataa
     8521 tccataagac aattgcaatt aaattaagag ccatcaaact tacaattatc gcgataacaa
     8581 aaagtataat cgataatttt ctacgtcctt taatacaaat tgtcgcaaat ataccataaa
     8641 gtgttgaaat taataaaata attgctaatg cacccattgt tgtctgaagc atatctaaag
     8701 aaaccttgcc accgttctga gataattgct tcgctaattc ttcacgttgt gatgcattac
     8761 cactttttaa agaaaaatag gatagcccca taactaataa ataaataata ctaatactat
     8821 ttgcaatcca agccatgatt aattcaggtt ttctattcat cttaaaatcc tttctttgtt
     8881 taatatttta ataagtatac tacgagccta tagatataag tacattaatt tacttgtata
     8941 ttaacatttt tttcatctaa tttttataat aaacatctga attcagattg atggtaattt
     9001 agctgtatgg taaagagaaa tcgtcattta aaattatgac acctaaaaac tttaattaaa
     9061 cgttatcgat aattaacctc atataaaaac ccagattaat aagcaaaaga cctctactta
     9121 ctaatctggg tatcatcaat atttattctt gctctttttt gtccttaact tcacttgaat
     9181 tgcgttcttc agaaatatca ttgttgtaaa tttgacgtcg ctcttcaaaa ttcattcgac
     9241 gttgactaac tgcgctaggt tgatttttcc tacgttcttt cattcgttgg ttataagctt
     9301 tacgtttttc ttttttctta gctttaattt cagctttttc tcgcttttgt tgtgctttca
     9361 attcttttgg atcaacttca ggttctgcat aataaccatc atcttcaata cctagttctt
     9421 cagtactttt ttgtttgtat ttagcctgtc tggataatac tgatggttct tcctcagtgc
     9481 gctcatcatg attatattca ctttcaactg cacgtttagg aaattgatca tatttatctc
     9541 tttccagttc ttgatcatat acagtgtttg atttatccaa ttctttttca tatttaggca
     9601 catcataata atcatctttc ggttgtgggc gctcatacac aggttcacga taatcataaa
     9661 tcggtccatt gtactcttca taatattgtg gcgcaaccat ttcaacttta tcttttctta
     9721 taaatagcat catcgcaata ataaagaata atatcggaat gattaaagta acgataagta
     9781 aaactaaagg aatcgttaca attgctgata ttaagaataa gaaacctgac aagattctta
     9841 tattcataga tatgagagct aaaaatgaaa tcaacaaaca aacaataaag taaacgataa
     9901 tagcccatat accattttgt aaccatatta caaattgtgt tgtattatag ccatttccag
     9961 ctaataattg ctggataaat gcattattgt ttaatgtatt ttcaagatta gcaatcgatg
    10021 tgttattact gaatgaaaca agtgctataa acatcgtaat gacagtaagc actaataaca
    10081 tcacccaaca taaccaacct agaacttttt cagttaatct acttactgga cgtttaattt
    10141 gagtaaattg ttctccagtc attcgttaca actccttata gtacttatcc cgttattata
    10201 actaaatata cagtaaataa ctactattta tgattttatt ttaatgacat tttgaaattc
    10261 aaaaagtttt cattgtattc acttaaaact tcaggtccta aatctttata aacttcaagg
    10321 cgttcttgct ctttcttagt cggatagaaa cgatggtcgt ctttaatctc tttaggcaac
    10381 aattgtcgag cagccttgtt tggcgttgca tagcctacga attcagtatt ttgcttgtta
    10441 tttttagcat ctaataaaaa attcataaat ttatatgcac cctctttatt ttgtgccgtt
    10501 tttggaatta ccatattgtc gaaccataaa ttcgatcctt ctttaggaat aacatagtta
    10561 tatttatccc cttcttgcac tagaggtgct gcaacaccac tccaaacaac cgcaatgtta
    10621 ccttcatttt gttcaagcat catggtaatt tcatcaccta cgacacctct tacttgtggt
    10681 gctaatttag ttaaatctcg ctctgcttct tttaaatggt gcgaattacg gtcattaaga
    10741 ttatacccaa gtttattcaa actcatgcct ataatctctc tagcaccgtc aactagtaaa
    10801 atttggtttt taaatttagg attatacaat gacttccaac tatcaaatga ttcatttgga
    10861 tacttttctt tattatataa aatacctaca gttccaaaga aataaggtaa agaatattta
    10921 ttgcctttat caaatgacat attcatataa tctgaatcta aatttttaat attaggtact
    10981 ttattatgat ctattggtaa caataaatga tctcttttca atttttgaac tgtatattca
    11041 ctaggaaaag caacatcata atgtgtacca ccattgcgaa ttttggcttc catcgcttca
    11101 tttgaatcga aagtttcata aacgacttga atgccagttt ctttttcaaa tttcttaatt
    11161 aactctggat caatatattc gccccaattg tatacgtaaa ttttttgatt tgtatgcact
    11221 tgttctttag atttaaacca atgacttaaa gtaagacaaa gcatacccac aactaatgca
    11281 cctataatga gttgtaaaaa acgtttcatt attttacacc tcgcttgatt agttttttct
    11341 tatttatcac gtattgaatc aaataatatc ctagtattcc taatacaata acagcaaaca
    11401 ataatgttga aatcgcatta atttccatac taattccttt tctcgccata gcataaactt
    11461 caactgataa cacactaaag ccattaccag taacgaagaa acttactgtg aaatcgtcta
    11521 gtgaataagt taaagccata aagaatcctc ctataataga aggtaaaata ttaggaataa
    11581 taatgttgct taataattgt ggttcagtcg ctcctaaatc tcttgcagca tttaacatat
    11641 tattattcat ttcatacagt tgtggtaaga cgataatcac aactataggt atgcaaaatg
    11701 caatatgaga tattagaact gtccaaaatc ctaaaccaag accagtaaaa tggccaatcg
    11761 ttgtaaacat aattaagaat gatgcaccta tgacaacgtc ggatgatacc atcaagacat
    11821 tattcaatgt tagtaaagtt actttaaact ttttatttct taaataataa atagcaatgg
    11881 caccaaatgt accaataact gtagaaattg aggctgctaa aagtgctaca gctatcgtgt
    11941 taaaaatgac cgacattaaa tgatcatttt gaaataatga ttgataatgc tctaatgtaa
    12001 aatgttcaaa gtgaatcata ttaccagccg aattgaatga atagaacatt aaaaagaata
    12061 ttgggatgta taaaatcgct aaaagtatcc cgatatacag ctttccatac catttcatat
    12121 gattcaccct ctcccattag atgattttgt aatgattaaa atgaatgcca taaatacaat
    12181 taagaatata gctatagttg atcccatacc ataattttga attgttaaaa attgctcctc
    12241 tattgccgta cctatattta tgactttatt acctgcaatt aatcttgtaa tcataaataa
    12301 tgaaagtgat ggaataaagg ttacttgaat cccagtcata acaccttctt ttgttaacgg
    12361 catgattact tttctaaaag tatagaatgg actagcacct aaatcacttg aggcttgcaa
    12421 taaattatta ggaattgctt tcatgctatt aaatataggt aaaatcataa atggtatata
    12481 aatataactt gccactacta aaaaggcacc agttgtaaat aacaaattga atgatggtaa
    12541 attaaataag tggaaaaatt gattaatcac accatcgtga cttaataaac ctataaaagc
    12601 atatgtcttc aacaataaat ttatccatgt tggaataatc attatcatta ataagatatt
    12661 ttgaaatttc gagcgagtaa tataataggc agctggataa ctgatagcca acgtaataat
    12721 tgttattaag gcggcatata aaattgaata tgcaaacatt tttaaatatt ttgtagtaaa
    12781 aatttgttga taattcgtga aactaaaatg tccattgata tctaaaaatg aaaaataaat
    12841 gagtaataca actggtacaa taataaatat aaccatccat agtaaatacg ggattaaaag
    12901 aaatttatta gtattacgca ttgtctactt cctcatagct ttcaatacgt ttatcaaact
    12961 cttcttctgt ttcaccagga accataatat gaatggcttc aggatcaaaa taaagaccaa
    13021 cttcgctgcc tacttcggct tttttagtcg tttgtattac ccattcataa cctttattgt
    13081 ctatacaaca tatttcatag tggacccctc taaacaacat agaatcaaca gttgctttaa
    13141 ataatccttc ttcagctttg attaatgata tatcttctgg tcgaataacg acttctactt
    13201 ttttattttc aggaataccc atatcgacac attcgaaatc ttgcccataa atattcacga
    13261 cataatctct aaccatgcgc ccttcaacaa tattagattc tccaataaaa tcagctacaa
    13321 atcgattcac tggttcgtca tatatatctg ttggtgtacc aaattgttga attttcccat
    13381 cttttaatac aaaaagaaag tcacttagtg ctaaagcttc ttcttgatca tgtgttacaa
    13441 atataaatgt aatacctaat ctagattgca attctcgtaa ttcatattgc atttcagtac
    13501 gcaatttcaa atctaatgcg gataaagatt catctaacaa taatatttct ggttcattta
    13561 caatagcacg tgcaattgca acacgttgct tttgtccgcc actcatttca ttaatattcc
    13621 ttttttcata acctgaaagc ttaactaatt ttaatgcctc agttactttt tgatcaattt
    13681 cggtttttga taattttttt aatttcaaac cgaaagcgat attatcatag acgtttaagt
    13741 gtggaaataa tgcataatct tgaaagactg tattcacttt acgtttattt ggtggtaaat
    13801 taccaattgg tttgttttga taaatcactt caccactgtc aggatattca aaccctgcaa
    13861 ttaattttaa aattgttgtt ttaccacaac ctgaaggacc taataatgta taaaagtatc
    13921 ctgattcaat atcaatatct atgtcatcta agatattaag atcatcatag cttttactaa
    13981 ctgattttaa tgataataac ggttccaaat aagatccccc ctataaatat gaagctgtcg
    14041 ctacaataag tattcgtgtt tcattgtttg attcatttga caaacgatga ttatctcgtg
    14101 ctttaaaata caaaacgtct tcttcttgtg cttgatatac ctctttgcct aaattaagtg
    14161 ttatctgacc tgacatacaa taaataaacg tatcagactc tgatggatta aaatttttat
    14221 atgatgcgcc aggctttaaa gttaatatta atggctccat atcatattca tttgactttg
    14281 aaactaacca atttaatata taaccttcat catactcatc ataaataact tgttcttcct
    14341 tcttgtataa tactttttca ttttcactgt ctttaaaaaa ttcacttggc gtcgtcccta
    14401 acacctctat aatatttaag aaagtttcca tacttggtga ggcatgttca ctttctattt
    14461 gtgaaatgta gcctttcgat aagtcagtac gttcagcaag ttcttcttgc gttaaatttt
    14521 taattcttct aagattttta attttattac ctatgttcat ttatctttcc acctaaaaaa
    14581 gctatattaa gtttactttt atcaaacatt ttgtttaaca cttgataaag gtatcaaaat
    14641 atagctttat tagcaatagt tttaaagaaa aaatcctatt aaattgtgtt aatttcgaac
    14701 gaattatttt ttagctgtta ctctttgttc ttcagagtga gtttttacat cttgaactgc
    14761 tttgtaacta ttatagccac tacttttttc gaaagtatta aaaatttgtt tttcctctgc
    14821 tttagcaggc acaatttctt taaatctttt atatgcatcc ataaagtctc ctgccgtcac
    14881 gccggattca taatacttct ctacatgatt aaagaaattt atcactaaaa tcatctcttc
    14941 attactccag tctaaatcaa ttggatactc atattccatt gttgaaccct ccatttcaat
    15001 tcttttgtta gcatcgctag tataacatgc atgtcacatg ttaacgatat ctcaaatcaa
    15061 tttccttaca ctactttatt acctaaaaca aaaaagccgt agcatatgct acagctttta
    15121 aaaactgaat ctttatagat aatcagttat tacattgtat ggattgggta tccgatagct
    15181 ttttcagctg cttccatagt catctcacct aatgttggat gtgcatggat tgttaatgcg
    15241 atatcttcag cattcatacc agcttcaatt gctaaaccta attcagagat aatatctgat
    15301 gcaccagtac caactacttg agcaccgatt aaagtatcat cttctttaag tgtaataagt
    15361 ttaacaaatc cgttagtatc gtctaatgat aatgcacgac catttgctgc atatggaaat
    15421 ttagaagctt taattgctaa accttcttct ttagcttgcg cttctgaata accaactgta
    15481 gctaattcag gttcagtaaa gcatactgct ggcataccaa tgtaatcaac ttcagcagct
    15541 tgaccatcaa ttgcttcagc agcaacttta gcttcatagc tagctttgtg agcaagtggt
    15601 aaacctggaa cgatatcacc aattgcatag atattgctga tagacgtacg gctttgttta
    15661 tcaacttcta ataatccacg gtcagcgaat ttaacaccta attcttctag gcctaattcg
    15721 tctgtgtttg gacgacgacc tacagttact aatacataat cagcttcgat tgttttctct
    15781 tcgcctttag cttcataagt aactttaact ccgttatctg tttcttcagc tgatttagcc
    15841 attgcttcag taacgatttc aacacctttt tctttcatac cttttttaac aggttgtgtc
    15901 atttgttttt cgaagccacc taagatatct ttagcacctt caaggatggt tacttctgaa
    15961 ccaaagttag caaatgctgt acctaattct gatccaatgt atcctccacc aactacaact
    16021 aatttacctg gtacttcttg taaatttaaa gcacctgttg agtcgataac acgtttaccg
    16081 aatttgaaat taggaatttc aattggtctt gaacctgttg caatgattgc gtttttaaag
    16141 ttgtatgttt gtgcgctctt ttcgtccata acacgtaagc tattgttatc tacgaaatat
    16201 gcttcacctt taacgatgtt tactttgtta cctttaagta agccttcaac accaccagtt
    16261 aatttattaa caactgatga tttgaattct tgaacttttt ggaagtttaa agaaacactt
    16321 tcagcaataa cacctaagtt ttcagaatgt tgtgcttcaa caaaacggtg agaagcatgt
    16381 agtaatgctt ttgaaggaat acatcctacg tttaagcaaa caccaccaag attacctttc
    16441 tcaacgattg ttactttttg tcctaattga gctgcacgaa ttgctgcaac gtatccacca
    16501 ggacctgctc cgattactat agtatctgtt tcaattggga aatctccaac taccatgttt
    16561 tacccctcca ttaataataa ttctggatta tttaataagc gtttaatgtg attcattgca
    16621 ttttggccag ttgcaccatc aatttgtctg tggtcaaagc ttaatgataa tgctaatact
    16681 ggtgctgcaa caatttctcc atctttaacg ataggttttt gagcaatacg gccaattcct
    16741 aagattgcta cttctgggtg attaataact ggagtgaacc attgtccacc agctgaaccg
    16801 atattactga ttgtgcatgt tgcacctttc atttcatcgg ctgttaattt accatcacgt
    16861 gctttaacag ctaattcgtt aatttcatca gaaatttgga aaatagattt acggtcagca
    16921 tgtttcacta caggtactaa taagcctcta tcagtgtccg ccgcaatacc gatattccag
    16981 taatgtttat gcacaatttc accagcttct tcattgaatg aagtattaag tgctgggtat
    17041 tttttcaatg cagaaacaag tgctttaaca acatatggta agaatgttaa cttagtacct
    17101 tgttcagctg cgatttcttt aaatttctta cggtgatccc ataatgcttg aacatcaatt
    17161 tcatccatta atgttacatg aggtgcagta tgcttagagt taaccattgc tttcgcaatt
    17221 gctctacgca tagcagggat tttttcagtt gtttctggga agtcgccttc taatgatact
    17281 gctgcaggtg ctgcaggagt ttcagcaact tcttcatttg tagctgaagc agctgattca
    17341 tttgaagctg ttggtgcacc accatttaag tatgcatcta catcttcttt tgtaatacga
    17401 ccatttttac cagatccaga aactgcttta atgttaacac ctttttcacg tgcgtattta
    17461 cgtactgaag gcattgcttt tacagttctg ttttcatcta cttcttcagt ttgagtagct
    17521 acaggtgctt gctctgctgg cgcttcctct ttcgcaggtt cttctttaga tgatgaatca
    17581 tcatcatgac ctttaaattg catatcttct gcatcaggtg catcgatttt aacaataacg
    17641 tcaccaacta cagctactgt accttcttct accataactt cttctacagt accagatact
    17701 ggtgatggga tttctactac tgatttatcg ttttgtacct cagctaaaac atcgtcttct
    17761 tcaatagtat ctccagcttt aacaaaccat tttacaattt caccttcgtg gataccttcc
    17821 ccgatatcgg gtaatctaaa ttcaaatgcc acgtttttgc cctcctaaga tttcgtaaat
    17881 taaacatttc attttaatca atgagactaa aatacgctaa cttcgttaac ttttacaatg
    17941 tattaaaatt ctaaagtttc ttttgctttt tcgatgatgt catttttgtt tggtaaccaa
    18001 acattttcag cttgagtgaa tggataaatt gtatctgctg ctgcaactct tccaataggt
    18061 gcttctaatg aaaggattgc acgttcactt aattcagcta caactgctgc accaacacca
    18121 gcttgacgtt gtgcttcttg aactacaact gcacgaccag ttttttcaac tgaagctaca
    18181 attgtatcaa tatcgattgg ttgaacagta cgtaagtcaa ttacttcaac agaataacca
    18241 tctttttcaa gttcttctgc agctttcatt gattcttgaa ccattgcacc gtatgtgatg
    18301 attgaaatgt cattaccttc ttttttcaca ttagccttac caatgtcaat tgtatattct
    18361 tcttcaggta cttcttcacg gaatgaacga tacaatttca tatgctctaa gtatacgact
    18421 gggtcattac ttctaataga agaaattaat aaacctttcg catcgtatgg gcctgaagga
    18481 ataacaacct ttagacctgg agattgagct aaaatacctt ctaagttatc tgcgtgcaat
    18541 tcaggtgtgt gtacgccacc accaaatggg ctacgaattg ttacaggtgc agttttagta
    18601 ccgcctgaac ggaaacgagt acgtgcaatt tgtccagcaa tcgcatcaaa tacttcgaat
    18661 acgaaaccta agaattgtac ttccataacc ggacggaatc cttcaactgc aagacccatt
    18721 gctaaaccac caatacctga ttcagctaaa ggtgtatcga atactctatc ttcaccaaat
    18781 tctttttgta gtccttcagt aacacggaaa acaccgccgt taacaccaac gtcttcacca
    18841 aaaattaaaa catcttggtc atttttaagt tcagttttaa gcgcatcatt aatcgcttga
    18901 accattgtca tttgtgccat ggcttacttc gactccttct ctttgtatat ttcatattgt
    18961 tctgctaagt tttgaggcat atcttcatac ataatttcca ttagagaagt aacagtttgt
    19021 ttttcagtgt tatcagcctc tttaattgct gctttaatat cagcttttgc acgttcaata
    19081 acttcatttt ctttgtcttc attccataaa cctttgtttt caaggaattt acggaaacgt
    19141 actaatgggt cttttttctc ccattcagca tcttcatctg aagttctgta acgagttgga
    19201 tcgtcaccag ccattgtatg aggaccataa cgatatgtca tagtttcaat taatgttggg
    19261 ccttcacctg caactgcgcg gtcacgtgct tctttagttg cttgatatac agctaacgca
    19321 tccataccat caacttggat accaggaata cctacagcaa ttgctttttg agctaatgtt
    19381 tcagcagcag tttgcttgct tcttggtgtt gaaattgcat agttattgtt ttgaataacg
    19441 aaaattgcag gtgctttata agctgctgca aagttaatac cttcgtagaa atcaccttgt
    19501 gaagaaccac cgtcaccagt gtaagtgatt gcaactgcat ttttaccacg ttttttaagt
    19561 gcaaatgcaa cgccagcagt ttgaatgtat tgtgcaccga taataatttg tgggcttaat
    19621 gcattaacgc cttcagggaa ttgatttcct ttgaagtgac ctcttgagaa taagaaagct
    19681 tcagttaatg gtaaaccatg ccaaataatt tgaggaacat ctctgtatcc tggtaaaatg
    19741 taatcttctt tttctaaagc gtattgtgac gctaattgtg atgcttcttg accagcagtt
    19801 ggtgcataga aacctaaacg tccttgtctg tttaatgaga tagaacgttg atcaaggata
    19861 cgagtccata ccattctttc cattaattct actaattgtt catccgtaag atcaggtact
    19921 aagtcttcat ttacgacgtt accattctca tccaaaattt gaaccatttc aaatttcgat
    19981 tgagtatcat ttaaaacttt tactgcatcg aattgggctt gtaacttagg agccattcaa
    20041 ttcaccatac ctttcccata attgtataga aattcatttt atccttacta attgtaccac
    20101 aaataaatgg gactgttaaa ccatttgaaa aagttctgtt ttaataaaaa aagatacaga
    20161 taacaggaac tgtattagtt gtttcctacc atctgtatca tattaattat ttaaattgat
    20221 caacgtcttg cttttcttta ccaaccttat ttagtacttt cgtatactta tctgatactt
    20281 cttttaactt tttatagttc tgttctattg cttttgattt ttcattaaca ccttgttgtg
    20341 tcgcatcatt ttgatttaaa tatttaaata atgttttttc tgagtttaca gcctttttat
    20401 atgctttcgc gtaatcactg tgcaacttat atttttcttt taatacatca tctaattgtt
    20461 ttacttcttt acgtttaaca tcattatcaa tattatcaac gtgacttttt gctttcttaa
    20521 agtcttgttc agacttctta attgcgtctt cttctttttc aaattccttt agacgatcat
    20581 cggcattttt aattaagtct tttactgctt taccgcgaac tgctgtatct ttactattga
    20641 catcttcagt taattttttc tttttctcat ctaattcagc aattttctta ccaacagttt
    20701 taattggttc ttcatcgtct ttaattttat ccacttgctt taaatatgcc ttaatttctt
    20761 ttttatccgt agtacatcct gcaagcaaga cactagatgc taatactact gcgattgttt
    20821 ttccaaattt cattacttgt acctccactt gctatttaaa ttaaatgata aataaaatct
    20881 gcttggaaat caattttaaa caattacatt acacatcttc attactaagt atatctgttg
    20941 acagttacgt catttataat ataattttta aagtcaagac gataaataaa tttctaaagt
    21001 atcattgcct gaaatcaaca ctttaatcat tttttaggca attagcatat ctgaaaattt
    21061 acatatacta tctaaaataa accttggcag tagttggtta actttgtaca tacttacaca
    21121 taaaaatgta tgagtatctt aacaaccttt ttactgtcat tcaactaaaa caaattaaaa
    21181 taaggaaggt gcaatatatg ttaacaatga aagacatcat tagagatggt catccaactt
    21241 tgcgtcaaaa agcagctgag ctagaattac cattaactaa agaagaaaaa gaaacattaa
    21301 tcgccatgag agagttttta gtaaatagtc aagatgagga aatcgcgaaa cgttacggtt
    21361 tacgttcagg cgttggtttg gctgcacctc aaattaatat ttctaaacgt atgattgctg
    21421 ttttaatacc agatgatggc agtggcaaat cttatgacta tatgcttgtg aaccctaaaa
    21481 ttgtaagtca tagcgttcaa gaagcttatt taccaactgg tgaaggttgc cttagtgtcg
    21541 atgataatgt tgctggtcta gttcaccgtc ataatagaat tacaattaaa gccaaagaca
    21601 tcgaaggtaa tgatatacag ttacgactaa aaggatatcc agcaattgtt ttccaacatg
    21661 aaattgatca tttaaatggt gtaatgttct atgatcacat tgacaaagac cacccattac
    21721 aaccacatac agatgctgta gaagtttaaa acacactatt actaattatc aaagcttagg
    21781 ataaatatga tgtcctaagc tttttctttt acaacttttc ctaaaaaata acagtaaaat
    21841 attttcacct ttctaccaaa ctttttatcc cctcatttaa attttaccgt ttcatataaa
    21901 atcctttaat tctttcttaa cattaattta taatctctac atttatttta ataaatagaa
    21961 ctgcacattt attcgaaata cttagatttc tagtgagata aactgcttta tttattatca
    22021 ttcatcatgt aaaataagat ttaactgaaa ttttagtgtt atttcactaa ttttttaaaa
    22081 tgaacgacat gatgaaccta gttattaacc aaatcgttat taagttacat tatagagatg
    22141 attggaatga atttatcgat atatactcca atacgatttt actagggtta acaataaatt
    22201 aaacaaacat tcttaggagg aatttttaac atggcagtat ttaaagtttt ttatcaacat
    22261 aacagagacg aggtaattgt gcgtgaaaat acacaatcac tttatgttga agctcaaaca
    22321 gaagaacaag tacgtcgtta cttgaaagat cgtaatttta atatcgaatt tatcactaaa
    22381 ttagagggcg cacatttaga ttacgaaaaa gaaaactcag aacactttaa tgtggagatt
    22441 gctaaataat gaaacaatta catccaaatg aagtaggtgt atatgcactt ggaggtctag
    22501 gtgaaatcgg taaaaatact tatgcagttg agtataaaga cgaaattgtc attatcgatg
    22561 caggtatcaa attccctgat gataacttat tagggattga ttatgttata cctgactaca
    22621 catatctagt tcaaaaccaa gataaaattg ttggcctatt tataacacat ggtcacgaag
    22681 accatatagg cggtgtgccc ttcctattaa aacaacttaa tatacctatt tatggtggtc
    22741 ctttagcatt aggtttaatc cgtaataaac ttgaagaaca tcatttatta cgtactgcta
    22801 aactaaatga aatcaatgag gacagtgtga ttaaatctaa gcactttacg atttctttct
    22861 acttaactac gcatagtatt cctgaaactt atggcgtcat cgtagataca cctgaaggaa
    22921 aagtagttca taccggtgac tttaaatttg attttacacc tgtaggcaaa ccagcaaaca
    22981 ttgctaaaat ggctcaatta ggcgaagaag gcgttctatg tttactttca gactcaacaa
    23041 attcacttgt gcctgatttt actttaagcg aacgtgaagt tggtcaaaac gtagataaga
    23101 tcttccgcaa ttgtaaaggt cgtattatat ttgctacctt tgcttctaat atttaccgag
    23161 ttcaacaagc agttgaagct gctatcaaaa ataaccgtaa aattgttacg ttcggtcgtt
    23221 cgatggaaaa caatattaaa atcggtatgg aacttggtta tattaaagca ccacctgaaa
    23281 catttattga acctaataaa attaatactg taccgaagca tgagttatta atactatgta
    23341 ctggttctca aggtgaacca atggcagcat tatctagaat tgctaatggt actcataagc
    23401 aaattaaaat tatacctgaa gataccgttg tatttagttc atcacctatc ccaggtaata
    23461 caaaaagtat taacagaacg attaattcct tgtataaagc tggtgcagat gttatccaca
    23521 gcaagatttc taacatccat acttcagggc atggttctca aggtgatcaa caattaatgc
    23581 ttcgattaat caagccgaaa tatttcttac ctattcatgg tgaataccgt atgttaaaag
    23641 cacatggtga gactggtgtt gaatgcggcg ttgaagaaga taatgtcttc atctttgata
    23701 tcggagatgt cttagcttta acacacgatt cagcacgtaa agctggtcgc attccatctg
    23761 gtaatgtact tgttgatggt agtggtatcg gtgatatcgg taatgttgta ataagagacc
    23821 gtaagctatt atctgaagaa ggtttagtta tcgttgttgt tagcattgat tttaatacaa
    23881 ataaattact ttctggtcca gatattattt ctcgaggatt tgtatatatg agagaatcag
    23941 gacaattaat ttatgatgca caacgcaaaa tcaaaactga tgttattagt aagttaaatc
    24001 aaaataaaga tattcaatgg catcagatta aatcttctat cattgaaaca ttacaacctt
    24061 atttatttga aaaaacagct agaaaaccaa tgattttacc agtcattatg aaggtaaacg
    24121 aacaaaaaga atcaaacaat aaataatcaa aaagctacta actttgaagt gaagttttaa
    24181 ttaaactcac cacacattgt tagtagcttt ttctttatat atgatgagct tgagacataa
    24241 atcaatgttc aatgctctac aaagttatat tggcagtagt tgactgaacg aaaatgcgct
    24301 tgtaacaagc ttttttcaat tctagtcagg ggccccaaca aagaagctga cgaaaagtca
    24361 gcttacaata atgtgcaagt tggggatggg ccccaacaca gagaatttcg aaaagaaatt
    24421 ctacaggcaa tgcaagttgg ggtgggacga cgaaataaat tttgagaaaa tatcatatct
    24481 gtaccactcc cgattatctc gtcgcaatat ttttttcaaa gcgatttaaa tcattatcat
    24541 gtccaatcat aattaaaata tcacctattt ctaaattaat atttggattt ggtgaaatga
    24601 tgaactcttt gcctcgttta attgcaataa tgttaattcc atattgtgct cttatatcta
    24661 aatcaatgat agactgcccc gccatctttt cagtcgcttt caattctaca atagaatgct
    24721 cgtctgccaa ctcaagataa tcaagtacac ttgcactcgc aacattatgc gcaatacgtc
    24781 tacccatatc acgctcaggg tgcacaaccg tatctgctcc aattttattt aaaatctttg
    24841 catgataatc attttgtgct ttagcagtta ctttttttac acctaactct tttaaaatca
    24901 aagtcgtcaa cgtacttgat tgaatatttt caccaattgc cacaatgaca tgatcaaagt
    24961 tacggatacc taaacttttc ataactgctt catctgtagt atctgcaaca accgcatgag
    25021 tagcgatatc actatattca ttcactctat tttcatcatg gtcgatggcc attacatcca
    25081 tgtctaatgc attcaactca cgaacgatac tacctccaaa acgacctaga ccgatgacta
    25141 catattcttt acccatactc gccctccatt aaatgatttt catcaattca ttgaaaatat
    25201 aaatttaaaa ttattataaa tgagtacccc aactaaatta tctaaatgca gtaatgcaag
    25261 taaatgaaag ttggggtatc gtctcaactt atgatttctt tccttcaaca tattctttgt
    25321 cgaaaacaaa taatcttaat aataatatta acgatggaag taataaaagt aaacctaaaa
    25381 taaagacaat cactaatgtc cagcccattt ctggattaac atatgcatct gtaattttta
    25441 caaacggata taaaaggtat ggcaatttac taattccata gccaaagaac gcgaacatca
    25501 tttgtaaaat aacaaataca aaagccaaac catgtttttt cttaaagaat gttaacaatg
    25561 aagctaatgc aaagaatagg aaacttatag caaacatcca ccagtagtca aaaacagctg
    25621 aataaaaatg ttcagaattt tgaatgcgta atgatagaaa tacgaataaa caaatgataa
    25681 tcatcggtgg ccctaaaaat atgtgccatt gtcttgttaa attatatgct ggttcgtcat
    25741 ttgctttttt agcataatat gtcaaaaatc ctgatgaaat atataaaact gaaataattg
    25801 ccaagaatac tacagaccaa gcaaatgggc ttaacagtaa ctgcacccaa tctagatcga
    25861 taacattgtt tcgaacatta atataaccac cttctgtaat agttaaagca gtagataatg
    25921 aagctggaat taacaatcca cttaccccat acatgatcat ccatgctaat tttgtatctt
    25981 gaccataatt ttcaaatgca taaaaactgt ttctcaacga tatcataatc agtgctattg
    26041 aacctggtat taacaatacc gtgcctaaat atttgattga ctctggaaag aaacctacga
    26101 atcctacgaa gaagaaaaca aagaatacat tcgtaacttc ccaaactggg ttcaaataac
    26161 gtgaaattaa gtgattaatt ttcttttcat caccagttaa ctttgaatgc aatgcgaaga
    26221 aacctgcccc aaaatctata gaagcaatga tgatatagca aaataaaaac aaccataaca
    26281 ctgttatacc tataaatgca taaatcattt ttctatttct cctccttgct tcttggctaa
    26341 acgatttaca tcttcatacg ccggtttatt tttaaacata cgaattaata cgtatgcaca
    26401 tgtatacatt aaaatggtgt acaatatgcc aaataaaatt gtaacaaagg ttattccgcc
    26461 tgcttgtgtt gctgcttctg ccacgcgcat ataaccacga acaatccaag gctgtctacc
    26521 catctctgtt aagaaccatc caaattctat agctagcatt gaagctggtc ctgttaataa
    26581 tattccgtaa agcatccatt tatgagtaga aaaatttcta agctttttaa acattaaagt
    26641 taaaacataa acacctgaaa tgacaaaaca taaaattccc atcgttacca ttaaatcaaa
    26701 gaaataatgg acgatcatag gcggatgtaa acttttcgga aaatcattta acccttgcac
    26761 tttagttttg acactattat ctgctaataa actcaatagt ccaggtaatt caatcgcacc
    26821 tttaacttgc tgagtctttt catctaacac accaaataat aataatttgg catgggaaga
    26881 tgtatcgaaa tgccattcat aagctgctaa tttttcaggt tggaatttat gcaaaaattt
    26941 tgcagataaa tcccctgcca acatagaaag taaagttgaa aagaatccaa caatcataga
    27001 cattttcaaa gctttcttat ggtagacagt atccttaggt tgacgattac gcaataattt
    27061 aaaagctgct attgatgcaa taacaaatgc catcgtcata ccggctgtag taattacgtg
    27121 aaatgatcga actataaacg atgggttaaa catcgcttct ataggttgaa cattgaccat
    27181 ctttccattc ttcaactcaa aacctgcagg cgtattcata aatgaattca ctgaagtaat
    27241 gaagaatgct gagaaagagc caccaataat tactggtata ctaattaaga aatgtgtcca
    27301 tttattttta aaacggtccc aagtatataa atatatactt aagaaaatag cttcaaagaa
    27361 gaacgcaaat gtttccataa ataatggaag tgcaataacg tgtccaccca tttccataaa
    27421 tgtaggccaa atcaatgata attgaagtcc tataattgta cctgtaacaa ctcccactgc
    27481 tacagtaatt gtataagctt tagcccatct tttggccata gctatatatt gaagatcatt
    27541 tttgcgaata cctaaaaatt ctgcaattgc gaacattaaa ggcataccaa caccaatcgt
    27601 tgcaaaaatg atatgaactg ctaaagtcat agctgtcaaa aaccgactga tttcaactgt
    27661 atccatttaa aaacatcacc tttttctttt tttgatgaca acacaatgaa cttaattata
    27721 attgctataa tgtgtatttt taaaacatta tgcgctatga aattgtgtat aatttatgtc
    27781 agttcacaat gtgttcacaa atttgaattt caatgtataa ttgtgtatat tacattagaa
    27841 taaagcacga aggagcatga tacatgtcag aaataatcgt ttatacacag aatgattgtc
    27901 caccttgtac atttgtaaaa aattatctaa atgagcgtca aattgatttt gaagagagaa
    27961 atatcaaaaa tcaacaatat cgaaacgaaa tgatagattt tgatgctttt tcaactccgt
    28021 ttattttgtt gaatggcaat ccaatgtacc atgt
//
LOCUS       DAKSQE010000022        27786 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.022, whole genome shotgun sequence.
ACCESSION   DAKSQE010000022 DAKSQE010000000
VERSION     DAKSQE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 27786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27786
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.022"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..683)
                     /gene="fmhC"
                     /locus_tag="O0V33_000834"
     CDS             complement(<1..683)
                     /gene="fmhC"
                     /locus_tag="O0V33_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HCX8975498.1"
                     /translation="MKFSTLSEEEFTNYTKKHFKHYTQSIELYNYRNKINHEAHIVGV
                     KNDKNEVIAACLLTEARIFKFYKYFYSHRGPLLDYFDAKLVCYFFKELSKFIYKNRGV
                     FILVDPYLIENLRDANGRIIKNYNNSVIVKMLGKIGYLHQGYTTGYSNKSQIRWISVL
                     DLKDKDENQLLKEMEYQTRRNIKKTIEIGVKVEDLSIEETNRFYKLFQMAEEKHGFHF
                     MNEDYFKRM"
     gene            complement(711..1829)
                     /locus_tag="O0V33_000835"
     CDS             complement(711..1829)
                     /locus_tag="O0V33_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCX8975499.1"
                     /translation="MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKS
                     KDFTRGYEQNVFAKSELNANKNTTKDKIKNEAVVKTSDTSLKLDNKSAISNGNEINQD
                     MKISNTPKNSNQGNNLVVNNNEPTKEIKIANLEAQNSNQKKVNKGTNNYFGYYSFREA
                     PKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKP
                     KTVPSNNKSNSNNSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGA
                     KDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKF
                     QSLDQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA"
     gene            complement(2056..2964)
                     /gene="sucD"
                     /locus_tag="O0V33_000836"
     CDS             complement(2056..2964)
                     /gene="sucD"
                     /locus_tag="O0V33_000836"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCX8975500.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYELLLTVNK"
     gene            complement(2986..4152)
                     /gene="sucC"
                     /locus_tag="O0V33_000837"
     CDS             complement(2986..4152)
                     /gene="sucC"
                     /locus_tag="O0V33_000837"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCX8975501.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            complement(4261..5028)
                     /locus_tag="O0V33_000838"
     CDS             complement(4261..5028)
                     /locus_tag="O0V33_000838"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCX8975502.1"
                     /translation="MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILTENPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPVTKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            complement(5012..5896)
                     /gene="ylqF"
                     /locus_tag="O0V33_000839"
     CDS             complement(5012..5896)
                     /gene="ylqF"
                     /locus_tag="O0V33_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCX8975503.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVGNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDL
                     ARLKAHYKIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            6297..8903
                     /gene="yfhO"
                     /locus_tag="O0V33_000840"
     CDS             6297..8903
                     /gene="yfhO"
                     /locus_tag="O0V33_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HCX8975504.1"
                     /translation="MLKKWLNSNVKQFLVITFMSVILTLILFSTHIYEYIVNGTVFSG
                     SGDGFRQMMPFQMYLYEHLSSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMVRAIITFVVTFYLFKILHFKRSTNMIATILYGM
                     STVVIYFNFTWSFYGNLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKLICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFFITISILTIVALLSFKLYRFYFYRLFAIVTWVLF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVSI
                     IALLYVLLSPTHPLALIVGIILQMVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSLSSLKQHDYHSNYVNQLIKKINKNATGPFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRV
                     NHDDNLPYGFKINSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNNTKDVNTHFKANKNLLSDSTIKLNSAAWQSPTKHLLQVKQNNGGLTVQLPKS
                     VSNQFKDLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRFVTPVTIRIKASDRI
                     RLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVRKTKQGYTITKNKNSSGYIVLP
                     TAYNQGMKATSGDQSLKVEQVNGVMTGIKAPKNITKIQLSYTPPYYYLLITITIFGII
                     CSIIFTRWARQK"
     gene            complement(9147..9497)
                     /gene="rplS"
                     /locus_tag="O0V33_000841"
     CDS             complement(9147..9497)
                     /gene="rplS"
                     /locus_tag="O0V33_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCX8975505.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(9600..10337)
                     /gene="trmD"
                     /locus_tag="O0V33_000842"
     CDS             complement(9600..10337)
                     /gene="trmD"
                     /locus_tag="O0V33_000842"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCX8975506.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQARVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNADKQILERYKIGLKKG"
     gene            complement(10337..10840)
                     /gene="rimM"
                     /locus_tag="O0V33_000843"
     CDS             complement(10337..10840)
                     /gene="rimM"
                     /locus_tag="O0V33_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCX8975507.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            complement(11028..11303)
                     /gene="rpsP"
                     /locus_tag="O0V33_000844"
     CDS             complement(11028..11303)
                     /gene="rpsP"
                     /locus_tag="O0V33_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCX8975508.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            complement(11738..13105)
                     /gene="ffh"
                     /locus_tag="O0V33_000845"
     CDS             complement(11738..13105)
                     /gene="ffh"
                     /locus_tag="O0V33_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCX8975509.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALLEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTKLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKDIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
     gene            complement(13131..13463)
                     /locus_tag="O0V33_000846"
     CDS             complement(13131..13463)
                     /locus_tag="O0V33_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCX8975510.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKKHLSNPEQIQRY
                     IQQLEDLE"
     gene            complement(13450..14700)
                     /gene="ftsY"
                     /locus_tag="O0V33_000847"
     CDS             complement(13450..14700)
                     /gene="ftsY"
                     /locus_tag="O0V33_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCX8975511.1"
                     /translation="MSFFKRLKDKFATNKENDEVKSLTEEQGQDKLEDTHSEGSTQDV
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLISRYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NEEITTVENDQIVTEEKDDNHGAK"
     gene            complement(14700..18266)
                     /gene="smc"
                     /locus_tag="O0V33_000848"
     CDS             complement(14700..18266)
                     /gene="smc"
                     /locus_tag="O0V33_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCX8975512.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVNDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLVERKKNQSETNARYEEEQENLIELLE
                     NISSEISEAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEVKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIHGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRAVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETHLESIKASLKRLEDEIERYTKLS
                     KEGKESLTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQSQQTKHQLNDVKEKIAF
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEATLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAMFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            complement(18413..19144)
                     /locus_tag="O0V33_000849"
     CDS             complement(18413..19144)
                     /locus_tag="O0V33_000849"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCX8975513.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            complement(19260..19493)
                     /locus_tag="O0V33_000850"
     CDS             complement(19260..19493)
                     /locus_tag="O0V33_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCX8975514.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            complement(19736..20470)
                     /gene="fabG"
                     /locus_tag="O0V33_000851"
     CDS             complement(19736..20470)
                     /gene="fabG"
                     /locus_tag="O0V33_000851"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCX8975515.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            complement(20463..21389)
                     /gene="fabD"
                     /locus_tag="O0V33_000852"
     CDS             complement(20463..21389)
                     /gene="fabD"
                     /locus_tag="O0V33_000852"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCX8975516.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLYNNNDQATEILTSAANTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPSGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            complement(21382..22368)
                     /gene="plsX"
                     /locus_tag="O0V33_000853"
     CDS             complement(21382..22368)
                     /gene="plsX"
                     /locus_tag="O0V33_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCX8975517.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSYELLNQDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKIAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            complement(22373..22945)
                     /gene="fapR"
                     /locus_tag="O0V33_000854"
     CDS             complement(22373..22945)
                     /gene="fapR"
                     /locus_tag="O0V33_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HCX8975518.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            complement(23148..25208)
                     /gene="recG"
                     /locus_tag="O0V33_000855"
     CDS             complement(23148..25208)
                     /gene="recG"
                     /locus_tag="O0V33_000855"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCX8975519.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFLNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYDLDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLI
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENRTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEIDVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            complement(25398..27044)
                     /gene="fakA"
                     /locus_tag="O0V33_000856"
     CDS             complement(25398..27044)
                     /gene="fakA"
                     /locus_tag="O0V33_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HCX8975520.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKLDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLADDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            complement(27059..27433)
                     /locus_tag="O0V33_000857"
     CDS             complement(27059..27433)
                     /locus_tag="O0V33_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCX8975521.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
BASE COUNT         8342 a         5390 c         3678 g        10376 t
ORIGIN      
        1 tgcattcgtt taaaataatc ttcattcatg aaatgaaaac catgtttttc ttcagccatt
       61 tgaaacaatt tataaaatcg atttgtttct tcaatagata aatcttcaac cttaacacca
      121 atctcaatag tcttttttat atttcttcta gtttggtatt ccatttcttt taaaagttga
      181 ttctcatctt tatcttttaa atccaataca gaaatccacc taatttggct tttatttgaa
      241 tatcctgttg tataaccttg atggagatac ccaatttttc ctagcatctt tactatcact
      301 gaattattat aattctttat tatcctacca ttcgcatctc ttaaattctc tattaaatat
      361 ggatcaacaa gaataaatac acctctattt ttataaatga atttagataa ttctttaaaa
      421 aagtaacaaa ctaatttagc atcgaaataa tcaagtaaag gacctctatg agagtagaaa
      481 tatttgtaga atttaaaaat acgtgcctct gttaataaac atgcagctat aacttcattt
      541 ttatcattct tcactcccac aatatgtgct tcatgattta ttttatttct ataattatat
      601 aattctatag actgtgtata atgtttgaag tgctttttgg tgtagttggt aaattcttct
      661 tcacttaaag ttgaaaattt catatttcaa tgtcctgcct tcgatttaaa ttatgctttt
      721 ttaaatggtc taataaaaat catatcattt tcataattat gaacaacttt atgtgcaacc
      781 tctgctttac gccatccacc attattccag ttttgatcta aactttggaa cttcattaat
      841 tttccatcat aatcaccatt taagacaata gctgtatgac catatcctcc accaaatctt
      901 ccactaaaaa ccactaagtc cccaggttca gctttgaaag ttggtgtgtt gtgataaatt
      961 ttagcttcac tattaaaatt atttgcatat ggtatatctt tagctccata tccttttaat
     1021 ccatgaccat aaagatgatt ccaatacaca ttgactaaat cgaaacattg ccatccataa
     1081 ctaccgtcga aatcccatcc tctattctct aatgttttca aataatttaa tgtactgtta
     1141 ttactattac ttttattatt tgaaggcact gtttttggtt ttggttctac taatggtgtc
     1201 attggcactt ttaatttttg accaataaat attaaattag gatttgctat attatttgta
     1261 ttttgaatat ttgaaactgt agttttgtat tttaatgcta tagcactaag tgtgtcacct
     1321 ttttttacag tatagatttg tgtttttgga gcttctctaa aactgtagta accaaagtaa
     1381 ttattagtac ctttattcac tttcttctga ttagaatttt gagcttccaa gtttgcaatt
     1441 ttaatttctt tagtaggttc attgttatta acaactagat tgttaccttg gtttgagttt
     1501 ttcggagtat ttgaaatctt catatcttga ttaatttcat ttccatttga aattgctgat
     1561 ttgttgtcta actttaaact tgtgtccgat gttttaacaa cagcttcatt ttttattttg
     1621 tcttttgtcg tatttttatt agcatttaac tctgatttcg cgaatacatt ttgctcatac
     1681 cctcttgtaa aatctttaga tttatcaatt tcatctgcat atgctttgtt cgacataccc
     1741 aatgccaaaa acatacctat tgaaattgac aaaattccaa tactaacttt tctaattgaa
     1801 tagcgtactt tactttgttg tttattcatg acgaaacact ccttacaata atatataaat
     1861 attataacat agtgcactat atattttaat aaaaatttgc tttgttttag aaagctattg
     1921 aaggaggtaa aataattaac ttgccttttc aaaatatgaa aagtgtactt aaattcatcg
     1981 cacgacaaat agcccatttc cgatactttt ataaagtatg gaatgggcta tagccattta
     2041 tatcatcttt taactttatt tattaactgt taataataat tcataaatac ctgcttcttt
     2101 agcagcttca attaatgttg aaccaatttc tgaaggtgtt gccgctgttt tcacaccaca
     2161 actatttaat gttttaattt tctcttcagc agtaccttta ccacctgaaa tgatcgcacc
     2221 agcatgtccc atacgttttc caggaggtgc tgtttgtcca ccgataaagc ctacaactgg
     2281 ttttgtcata ttcgctttaa tccattcagc tgcttcttct tcagctgtac caccgatttc
     2341 accaatcata acaactgctt tcgtttcgtc atcttcattg aatgctttta aaacatcaat
     2401 aaagtttgtg ccatttactg ggtctccacc aataccaaca gctgtagttt gaccaatacc
     2461 ttcttcagtc aattggtgca ctgcttcata tgttagtgta cctgaacgtg atactacacc
     2521 aacatgacct tttttgtgaa tatagccagg cataatacca attttacatt catctgctgt
     2581 aatcacacct ggacagtttg gaccaactaa acgtgttttt ctaccttgta aatagcgttt
     2641 aactttaacc atgtctaata caggaatatg ttcagtgata caaataacca tatctaagtc
     2701 tgcatcagct gcttctaaaa ttgagtctgc agcaaatggt gctggaacgt aaatgactga
     2761 aaccgttgcc ccagtttcat ttttagcttc ttcaacagtg ttgaaaacag gaacgccttc
     2821 aacaacttga ccacctttac caggcgtcac acctgctact attttcgtac cataatcaag
     2881 catttgtttt gtatggaaaa gggcagtaga ccctgtaata ccttgtacca ttactttagt
     2941 attcttatct ataaatacac tcatcttagt gctcccatcc tttccttatg cttctttgac
     3001 tagtttaaca attttttgtg caccttcagc cattgttgct gctggttcaa ttgctaatcc
     3061 tgagtctttt aagatttttt tacctaactc aacatttgta ccttctaagc gtacaactag
     3121 tggtaaagtt aaatctactt cttttacagc ttcaacgata ccttctgcga taacatcaca
     3181 tttcataatg ccaccgaaaa tgtttacaaa aataccttta acattttcat cacctaaaat
     3241 gattttaaat gcttcagtta ctttttctct agtagcgctt ccgcctgcat ctaagaaatt
     3301 ggctgggttt ccaccgaaat gattaatcgt atccattgtt gccatggcta aacctgcacc
     3361 attaaccata catccgatgt caccatctaa tgcaatgtat gataaatcat gtttagacgc
     3421 ttcaatctct ttcggatctt cttcttctaa atcacgtaat tctacaacat ctttatgtct
     3481 gaataatgca ttatcatcaa aattaatttt agcatctaat gccaatacat caccatcagc
     3541 tgttgtaact aatgggttga tttctacgat tgaacaatct ttttcaatga atacattata
     3601 aagtgctaat aagaatttag cagctttgtt aacagattct ttaggaatat taatattaaa
     3661 cgcaattcgt cttgcttgga atggtgaaag tccgattact ggatcgatag tttctttgaa
     3721 gatcttttca ggagtcttcg cagcaacttc ttcaatctca gtgccccctt cttcagacgc
     3781 catcaatgtt acttggtcag tcgcacgatc aataacgaat ccaacgtaat attctttttg
     3841 aatagcacaa ccttcttcga tatataaacg cttaatttct ttaccttctg gaccagtttg
     3901 atgtgtcacc aaagttttcc ctaataattc ttttgcatat gtttctacct cagataaaga
     3961 tttagcaatt tttactccgc ctgctttacc tctacctcca gcatgaattt gtgctttcac
     4021 aacatataca tcagaattta attcttttgc tttctccacc gcttcttcag cagtaaatgc
     4081 tactcgtcct tctggaactg caacgcccat tgaacgaaat atttctttac cttgatactc
     4141 gtggatattc atcttccatc ctcctgttac ttaggttaag ttcccttaca attataaaaa
     4201 atgtaagcgc tattgtaaac ttaaatgcta cttttttatc atttaattga attttacgat
     4261 ttacagtaac gattttatag gttcaaagct ttttctatgc tctttcataa tgccaatatc
     4321 atcgattgct agtaaatgtt gtttggtacc gtaacccgca tttttttcaa aaccatattc
     4381 aggataatct ttagataact gtgtcatata atcatcacga aaaacctttg ccatgatgct
     4441 tgcagctgca atagacacac ttcttgcatc acccttgatt aaagatactt gaggcagtgc
     4501 attatcaagc gtcatcgcgt ctatcaataa atgcgttggt tgtactgata atccatcaat
     4561 agctcgctgc atggcgattt gagtagcttt ataaatatta aattcatcta tttcttcagc
     4621 tgtcgcgata ccatatgcaa aagcagtaac ttcatttttt agtgcttcat ttaattctag
     4681 acgtttcgta acaggtactt ttttcgaatc atcaaggccc aaataattgt gatttgaatt
     4741 taaaattgtt gcgcatgcaa cgactggacc tgctaaaggt cctcttccaa cttcatcaat
     4801 accacaaata atagcattag gattctctgt taatatttca ttttcaaagt aagtcatttc
     4861 aacatacttt tcttttaaag cttgttcttt ttctaacgct tttcttcgtc tggctatggc
     4921 attttgtaca ccttttcgct catctaaaaa gcattcatga ttttctaatt cttctattgt
     4981 attaaccgca ttaatcaact gcgttacttc tttaattgtt agcgtcattt gctaattcct
     5041 cagtcatatc tttaaaaata tcaaaacaat aatttcctat tttagcattt cgaatatcat
     5101 aaataatcag ttcaatgact gcttcgtaat caatttcatt accacgtcga attaagccac
     5161 gttttttccc tatcgcatca aaccacgcta tgatttctgc atcttcagga acttcgattt
     5221 tataatgtgc ttttaagcgc gctaaatcat tttgaattaa aaagttcagt ccatagatgg
     5281 caacttcatc taagtgcaca atactatctt taatcgcgcc tgttaaactc aacttcttac
     5341 cgacctcttc atcttcaaat ttaggccaaa gaatccctgg tgtatctaat agttgtaatg
     5401 cattaccaac tttaatccat tgttgttgtt tggtcacacc tggtttatta ccagtctgtg
     5461 caatactacg ctttgccagt ttatttatta atgtggattt accaacattt ggaattccaa
     5521 caatcatcgc tctaatcgct cttggcttaa gtccttttgc tttttcacgt tcaaattttt
     5581 cagcagtcgc cttaattgct gcagcttcca ctttctttaa atttttaccg tgcttagcat
     5641 ccactgatac aggatagtat cctttatcaa taaaaaattg ttcccatttt gacatctcat
     5701 ttaaattaga catatctttt ttatttaata taacaacacg tggtttttgg ttaataactt
     5761 catctatcat agggtttctt gaactatatg gaattcttgc atctactagt tcaaacacta
     5821 catctacttt ttttaattgt tcacttactt ctcttttggc tttcgccata tgtcctggat
     5881 accattgaat aaccattcaa ttcaccttac tttcgtcaat tcttttctca ttattaaaat
     5941 ctataacttg ttaattatag aacaaattca ttaattcaac tatatttatt ttaacattgt
     6001 cacacttctt aacaatgctt tagattaagg tataagtgat tattccaagt tttctctaat
     6061 taaaaattat catttattaa atctaagctg cttattaggt gatattcaat taatacagta
     6121 tgacattttt attattcaat atatttgttt aatgataaac taaaaataat ccaaagttta
     6181 agtaactatg ataaggtatc tgttccacag tcatctttgt ctttacctat cgagaacctc
     6241 attaacttta gtttcagtta aacttaattt taaatttgaa agttgaggat aatgttgtgt
     6301 taaaaaaatg gttaaattca aacgtcaaac aattcttggt cataactttc atgagcgtaa
     6361 tattaacgct tattttattt tctactcata tctatgaata tattgtgaat ggtactgttt
     6421 ttagtggatc tggagatgga ttccgtcaaa tgatgccatt tcaaatgtat ttgtatgaac
     6481 atctaagtag tttttctagt ttatatgatg catcgtttgg attaggtggc gattatatga
     6541 aaggactatc atattattat tcgctgtcac ctttaatgtg gctcaatttt ctattcatta
     6601 aaataggaga aactgttggt atatttaatc cgacaacaat acatttttgg ccgacaaacc
     6661 aacttattat ggctatggta cgagctatca taacatttgt cgtgaccttc tacttattta
     6721 aaatattaca ctttaaacgc tcaacaaata tgatcgctac gattttatac ggcatgtcaa
     6781 ctgtcgttat atactttaat tttacttggt cattttatgg aaatttatta tatttattgc
     6841 cattatcgat tcttggtttg gaaagatatt ttcaacaacg caaaatcggt attttcattg
     6901 ttgcgatagc cttaacacta tttagcaatt tttatttcag ttattatcaa gctattatta
     6961 taggttgcta ctatttatat cgactcattt tcacttacaa atatgacatt gtctctagaa
     7021 cacaaaaatt aatttgcgtc atatctgcta cagttttgag tgtgttatca agtgtatttg
     7081 gtttattcac tggcatttct gcgtttttgg aaaatgacag aaagcaaaat cccaatgttg
     7141 atataccgtt tttgacacca cttgattatc attatttttt ctttagcgat ggatttttca
     7201 ttacgatttc aattctcact atcgttgcat tattgtcatt caaactgtat cgtttttact
     7261 tttatagact tttcgcaata gtaacatggg tattatttat tggttcatta tcacagtatt
     7321 ttgacagtgc ttttaatggt ttttcatttc cagaaaggcg ttgggtgtat atcttagcac
     7381 tatcatcaag tgctctttgc ggattgttta ttcaacattt atcaacatta aatatgaaat
     7441 attatttaat cagaacaata ccagtaagca tcatcgcatt actttatgta ttactatcac
     7501 cgacacaccc actcgcactt atagtaggta ttatcctgca aatggtgctt gctgttattt
     7561 taaaatttag tttatggcgt tataaaaaat taaccgttgc aatattagta ttaatcgtta
     7621 tgattcaaca aatcgtcatt ttagataaca acaaaaatat ggcaatcaaa ccttatcaac
     7681 aatcattatc atcgttgaaa caacatgatt accatagtaa ctatgtaaac cagcttataa
     7741 aaaagataaa taaaaatgca acaggcccat ttaatcgcat tgattatatg tcagactatg
     7801 cattaaattc accatttata tatcattata atggcatttc attatattct agtattttta
     7861 atggagacat tttaaaatat tatgacaaga cactccaaat taatatgcca atcgataaaa
     7921 acagcactta tagactactt ggcaatcgtc aaaatttact atcactttgg aatgttaatg
     7981 atcgaattag agtgaatcat gatgacaact taccatatgg atttaaaatt aactctgaac
     8041 acaaagacaa taaagttcgt tggattcatt ctaaaaatac catccattat ccaagtgcac
     8101 atattacaaa taaggtcttt tccaataaag aattaaaatc tccattagat aaagaacaag
     8161 caatgttgca agggattgtt tctaacaata ctaaagatgt taatacacat tttaaagcca
     8221 ataaaaattt actatcagat tcaacaatta aattaaatag tgcagcctgg caatctccta
     8281 caaaacattt attacaagtt aaacaaaata atggtggtct aactgtacag ttgccaaaat
     8341 cagtttctaa tcaatttaaa gatttgtatt ttgaaatgga tttagaatta ctttcgccgg
     8401 ataaagctca tgatgttaaa gtgaatgaat atacacaaga aagaaataaa ctcacttata
     8461 aatatcgacg ctttgtaaca cccgtaacga tacgcattaa agcttcagat agaattagat
     8521 tatcattgcc taaaggtaag tatcgtgtaa atttaaaagg gatatacggc gaagattata
     8581 ccacgcttaa agacgcttca aattcattag aggctgtcaa agttagaaag acaaagcaag
     8641 gttatactat tactaaaaat aaaaattcat ctgggtatat tgttttgcca acagcatata
     8701 atcaaggtat gaaagcgaca tcaggtgatc aaagtcttaa agttgaacaa gtaaatggtg
     8761 ttatgaccgg cattaaagca cctaaaaata taacaaagat tcaattgagc tataccccac
     8821 catactatta tttacttata acaattacta tatttggcat tatatgtagt attattttca
     8881 cgagatgggc aagacaaaaa taatgttgct acgcacatat ttaataaatc tactaagtcc
     8941 tttttcatgg cacttagtag atttttgttt atataaccat tttgagcaaa cacgttatga
     9001 ataaagaagg gaaaaacaat tacattatca actttacatt aataaaacag caaaaaaagc
     9061 catctatatc actccataaa agtgacctag atggctttat tgattgagtc gaatttgact
     9121 catacatagc tttgtttaaa tgctgattaa cgaatttctt ggattctagc agctttacca
     9181 cgtaaactac gtaagtaata taatttagca cgacgtactt taccacgacg tttaacttcg
     9241 attttttcaa tttttggtgt gtgtaatggg aatgtacgtt ccacgccaac acctgatgaa
     9301 attttacgaa ctgtaaacgt ttcagaaacg ccaccgccac gacgtttaat tacaacgcct
     9361 tcgaatactt ggatacgctc acgagtaccc tcaatgatac gtacgtgtac acgtaaagta
     9421 tcaccaggac ggaaacttgg taagtctgta cgtaattgtg atttagttac tgcttcgatt
     9481 aatttgtgat ttgtcattat aaattctcct cttcaaccta tgttcttgcc tcgacaaata
     9541 tatagcagcg gatcatagtg atttttgtgt ttccacactc atattatgtt agcacaatac
     9601 taaccttttt tcaatcctat tttatatctt tctaatattt gcttatctgc attagttaat
     9661 ggatattttt caattaagtc aggtctttta ttatatgtgc ggatcaactt ttgctcatgt
     9721 ctccatgcat caatattggc atgatttcca gacaataaaa catctggaac tgttagaccc
     9781 ttaaattcac gcggacgtgt atattgcgga aactctaata acccatcaga aaatgaatca
     9841 tcttgatgtg actgttcatt acctaaaaca cctggaatca gtctaacaat agcatcagtc
     9901 atggtcatcg ctggcaattc tccacctgtt aaaacatagt cacccattga tatttcatct
     9961 gtgacaagat gtgttcggat acgttcatcg taaccttcat aatgtccgca tatgaaaacg
    10021 atgtggtcag ccttgcttaa ttcaacagct ttctgatgtg aaaatggctc gccttgtgga
    10081 cacattaaaa taacgcgtgc ttgttctgtg acatctaagt cttccatcgc attaaaaaca
    10141 ggttcaggct ttaacaccat accttgtccg ccaccatacg gataatcatc tacttggttg
    10201 tgcttgttaa ttgcataatc tctaaaatta accgtattga tttgtaattt attgttttct
    10261 tgggcacgtt tcataattga atgatttaaa acaccatcaa acatttcagg aaataaagtt
    10321 aaataatcaa ttttcattaa tccaacaatc cttccattgg cgtgatgata atttttttat
    10381 tttcaacatc cacttctttt acaacatcag caatataagg aatcaaatat tctttagatc
    10441 ctttaatcac ccacacatca ttcgcacctg tttcaaatat attaattaca cgacctattg
    10501 gtgtttcttg atcatcaaaa actgtacatc ctataatatc tgaataataa aattcatttt
    10561 cctcaagtac gatatcttca tgatcacgct cttgataaat agaactccct tttaaatgct
    10621 caatatcatt aatattatta ataccttcaa atgtgagcat atgaagccct ttatgcactc
    10681 tatgtgactt aacagtatat tcaaggtcgt tattgttatg cacaactgtc agcacttgac
    10741 cgggttgaaa acgaacgtct gtaaagtctg aattggattt tactttaatt tcacctttaa
    10801 taccatgtgt gttaacaatt tgaccaactt caactctcat tctattacct ccaactctat
    10861 gctttgaaca cttctatttt aattgaaaat aattcaaaga aaaagggcac cattatggta
    10921 cccaatacgt tctatattac taacacgtga tattcccaac gtatcattga gtaataactg
    10981 tcagtgtaaa cgagttattc ttattgttag aacaatttta cgctaaatta cttagctttc
    11041 ttttgttcgt caaatttttt cataatacct tcttttgata agatattgtg aactgtatca
    11101 gttggtttcg caccatcatt taaccatttt aaagctaacg cttcgtcaac tttaatttct
    11161 ggagcattag cgctcgttgg gttataagta ccgatttgtt cgatgatacg tccgtcacgt
    11221 ggagaacgag catctgctac tacgatacga tagaatggat ttctttttga acctaaacgt
    11281 gttaaacgaa ttttaactgc catttataaa atctccttta agtcatgttt taatttttat
    11341 tttctacaag aaataatatt aacaggtgtg tgaatgtttg taaagagttt tttctttacc
    11401 tagaaaatta ttgaaccttt tccttacaaa aataactatg tgattactaa attagactgt
    11461 taataggtta taaaaattca tagtagcctt caccacatac gtgtttagtt aatttatccc
    11521 gtaaggacat gaatggtacc acttctaccg cacacgttct cccaatataa cttattaata
    11581 ttagcaacaa taacctcatg ttctagcaaa tcagcaattt cagtatttaa ttattattgt
    11641 gtatctaaaa atatgcaccc cataaagtta tattctttag tataactttc gggatgcaca
    11701 tctcattgca acaaaaatca gcatgttaaa ttaaacatta aaatggtaag ttcatacctt
    11761 ttaacatatt ttgcatttga ttgcgtttac cttttttacc tttaccgcca ccagtgaatt
    11821 gtttcatcat tttcttcata tcgttaaatt gtttcatcaa acgattgact tcttgtaatg
    11881 aacgtccaga acctttagca atacgctttt tacgtgatac attcaatgtg tctggattgt
    11941 ttctttcagc cggcgtcatt gactggataa tcgctttaat atgatcaatt tgcttttcac
    12001 tcatattaag cttatctagc cctttcattt tattcatacc tggaatcatt ttcataatat
    12061 catccagtgg tcctaaattt ttcacctgat caagttgttc taaaaaatca tctaaagtaa
    12121 atgatgactc acgcatcttt ttctctaaat cttttgcttt ttcttgatcc acatcttgtt
    12181 gtgctttttc aattaaactt aacacatcac ccatacctaa aatacgtgat gccatacgtt
    12241 caggatggaa tagctctaaa ccatctaact tttcactcat accaacaaat ttaattggtt
    12301 tttgtgtcac cgaacgaata gataaagctg caccaccacg tgtatcacca tctaatttag
    12361 ttaaggtaac acctgtgaca tcaagttgat cgtcaaatga ttctgcaaca ttgacagcat
    12421 cttgacccgt cattgaatcg acaactaaca taatttcgtt tggtttagca atgtctttta
    12481 cttcttttaa ttcgttcatc aatgcttcat cgatgtgtaa tcgacctgct gtatcaatga
    12541 ttacaaagtc taaatgttct tctttagcat gttttaatgc attagttaca atttgttgtg
    12601 gctttacttg atccccttca ctgtatacag gaatatcaat ttgttttcct actgtttgta
    12661 attgatttat cgctgctgga cgataaatat ctgctgcaac taacattggt tttttgttgt
    12721 attttttacg catcaataat gctaatttac ctgcagttgt tgttttacca gcaccttgta
    12781 aaccaaccat cataacaaca gtaggtggtt tatttgacat attaatcgat gtattttctc
    12841 cacccatcaa cttcgttaat tcatcttgaa ctattttaat aacttgttgc cctggtgtta
    12901 atgattgcat tacatcggaa cctaatgcgc gttctgatac tgttttaata aattctttta
    12961 ccactttaaa gtttacgtca gcctcaagta acgctaatct tacttcacgc atcattatct
    13021 ttatatcagc ttcagtaagt ttacccttac cacgcatttt ttgcatcgtc gcttgcaggc
    13081 gttctgataa gccttcaaat gccatatcga tgtcctccct caatttatat ctattctaag
    13141 tcttctaatt gttgaatata acgttgtatt tgttctggat tacttaaatg ttttttcatt
    13201 tcatcatata tttctcggcg ttgctcaaat ttctggtata attccaattt cttttcataa
    13261 tcttcaacta aatcgccagt tcttcttata ttatcataaa ctgcttgtct actcacatta
    13321 aaagtatctg cgatttcact taaagaataa tcttcgagat aaaataattc caaataatta
    13381 cgttgtttat tcgtcaataa ggattgataa aaatcaaaca aataattcat tcgtaacgtt
    13441 ttaactaaat cattttgccc catgattatc gtccttttct tctgttacaa tttgatcatt
    13501 ttcaactgtt gttatttctt cattttgttc aatcatatca gcgaataagc cgtagacata
    13561 actttcaggg ttaaatggtt gtaagtcatc taattgctca cctaaaccta catatttaac
    13621 tggaatgtgc aattcattac gaatggctaa tacgatacca cctttggctg taccatctaa
    13681 tttcgttaat acaatacctg taacatttgt tacttcttta aagtttctag cttgtgacaa
    13741 cgcattctga ccagttgtag catctaaaca tagtaatgct tcatgaggcg catctggcac
    13801 tgctcgatta attacacgct taactttttc taattcttgc attagatttg ttttattttg
    13861 taaacgtcca gcggtatcac agattaaaat atcaacacct ttgtttttag cggcattaat
    13921 cgcatcatac ataacagcag ctggatcaga accttcactt tggctaatta catctacacc
    13981 aacacgttcg ccccaaactt tcaattgatc aatagcaccc gctctaaaag tatcgcccgc
    14041 agctaacatt acttttttac cttccatttt atatcggtaa gctaattttc caattgttgt
    14101 tgttttacca acaccattca caccaaccat taaaatgaca tttaaacgac catcttctaa
    14161 gttcatagct tctgaattat catcttcttg atggtaaatc tctacgattt tttcaacaat
    14221 gacttcacgc aaatcttcag tatcttgaat attacgtcgt tgtgcttcca tacgtaattc
    14281 ttcagttaac gtcatcactg tattaaaacc gacgtctgca gtgattaaca tttcttctaa
    14341 agcttcaaaa aagtcttcat ctactttacg atatctcgat atcaaattat ttaattgttc
    14401 ttggaaattt tgacgagatt tttcgagtcc tgctttaaat ttagcaccca ttttttgagc
    14461 ttcaatttct tcaaaatctt caattgatat taagccatcg tcatcaaaat ccgcttcact
    14521 caacttgcgt ggcttctttt tcacttcagc attttctgct aaatcattta cgtcctgcgt
    14581 tgaaccttca gaatgtgtat cttctaattt gtcttgacct tgttcttctg ttaaggattt
    14641 aacttcatca ttttctttat ttgttgcaaa cttatctttt aagcgtttaa aaaagctcat
    14701 tattgctcct ccttcaacac atcatctatt gtatttaaat tcacactcac aagtttagta
    14761 acacctgatt cttgcattgt tacaccgtat aacctatctg caaattccat tgttccttta
    14821 cggtgtgtaa taacaatgaa ttgtgtttcg tctgataact catttaaata ttttgcgtat
    14881 ctaataacat tcgcttcatc taatgcagct tcaacctcat ctaatataac aaaaggtgca
    14941 gatcttactt ttaaaattgc aaatagtaaa gcaatagcag ttaatgcacg ctcaccacca
    15001 cttagtaacg ataaatgttg caacttttta cccggtggtt gtaccacaat atcaatacca
    15061 gctgttaaat aatcggcttc agttaattgc aattctgcat cgcctccacc aaacaattgt
    15121 ttgaacatag ctgtaaaatg tccttgaata gcatggaaag tttctttaaa tctttcagta
    15181 acctcttgat ccatttcact tataatttgc tctaatgttt ctttagcttt acgaagatct
    15241 gtacgttgct cacttaaaaa tgtataacgt tcatttaact cttcaaattg ttcaattgca
    15301 tttaagttta caggacctag ttcatcaatc gacatcttca ttaatttaac ttttttacgt
    15361 aatgcgtcaa tcgattcatc actcgtatat tcagattttg cacgttcaac agtcaattga
    15421 tattcatcat ttaaatgatc tatcgcatga tgaattaata catctagctt tgattgttca
    15481 gctttaatat cttggtagtg attttcgata gctaaaatat cttggtgaca aacttgtagt
    15541 gtagcttctt gcgcatcgat ttgttcattc aactcaatac gttgttgttt caatttatct
    15601 aattcatcag ataagcgtgt tctcgtttct tgttgaccat taatttgatc tttaatattt
    15661 tgaaaagctt gttcgcccat cacttcatcc gaattaaaga atgctatttt ttctttaaca
    15721 tcatttaatt gatgtttggt ttgttgactt tgattattta atcgatctat tgtctgttgt
    15781 tgtgttttaa tacgctcttt aaccacagca agatcagatt gtttctgatg taacgtttgt
    15841 tgtgttttag ttaagctttc cttaccttct ttagaaagtt ttgtgtagcg ttcaatttca
    15901 tcttctagtc gttttaaaga tgctttaata ctttctaaat gagtttcttt ttcactcaaa
    15961 gtttgtcgac ttttgtcact cgtataacca tcattttttt caaattcaaa ttcttcatga
    16021 tcatttttta tttgtgtttc ttgtgtagtt aatctatcga gctccatttc aaaatgatgc
    16081 acttgctctt taagtgtatt atgcttttga cttttttcaa aatacagttc acttaattga
    16141 tcacttttta tcttcaactc tttaaattgt tgttcaaatg attctgtttg acgcaagtaa
    16201 tcttctaatt ggtgtctcat tgttgtcaac tcgtcttttt gagacagaat actttttgac
    16261 ttacgagcgc caccaccagt catagaacca ccaggattta caatatcacc ttccaaagta
    16321 acaatacgag ttcgatattt aatcgcacgt gccaattcat ttgcatgctt taaatgatca
    16381 acgataatcg tattacctaa taaattcccg ataatatttt gatattctgg tgctacttta
    16441 actgcttccg aagcaatact aataaatccg tttgcctctt tagcaataga cttaatatca
    16501 gtcgctaccg ctctactctg tataacattt aatggtaaaa acgtcgcacg acctaaatta
    16561 cgttctttta aaaattgaat agcctggcgt ccatcttttt ctgaatctac aatgacatgt
    16621 tgtaatgaag cacctaatgc tgtttcaatt gcctgagtta atttagatgg cacatcaata
    16681 atttccgcaa ctgcaccatg aatacccttt aattctttat ttttagcttt caaaatatgt
    16741 ttgacgccat tgaaaaaata agtatattct tcctcttgcg ttgccaaact atcaatacgt
    16801 gttttcattt tttcggtata tcgatatgct tgatacaatt tctcttcgta ttcgttttgt
    16861 gctttttttg tatcagtgag gtctttttct atatttttaa tttctttatc tacagcagaa
    16921 agttctttgt tggtctgttg atattctttt ttagtcgttt ttatttgacc ctgaatatct
    16981 ttcaattgct caaagacttc aactaatcga gaatctagtc ttgatttttt aacctcattc
    17041 tcttctatag tatgctttaa aaaacgaata tcattgttaa catctgattg ctctgacatt
    17101 aatgtatagt attcgttttt aatttcttcc aatttttcat cgtgtgcttc gtctgaaaca
    17161 tatagttgtt cttcaagttc acgaatgaca gcattgagtt ctttttgttt acttttcaga
    17221 gacttataag tatcttgagc ttcagaaatc tcacttgata tattttctaa aagctcaatt
    17281 aaattttctt gttcttcttc atatcgtgca tttgtttcag attgattttt cttacgttct
    17341 actaaaacat ttaattgtcc cgtatatttt tcaaaggctt ccgtagcttt tactaattga
    17401 taattaagcg attcaacatc attatcaagt tgatgacgct tacctttata ttgttgaatt
    17461 tgttggctta aacgttgctt gtcagcttct ttatttgctt gttgaccttg taaatcattt
    17521 aaacgttgat ctaattgtct attgtcattt gtatattgat caatatcgtt cactgtaact
    17581 acaatgtcac tatgtttcat ttggtgtgaa agtgtcttat attctttagc tatagctgcc
    17641 tcctctttta gaggttctac gcgaccttcc aaatcatata aaatgtcttc tactctcgtt
    17701 aaattatctt ctgtttggtc aagtttattt aatgattcag ctttacgttt tttatattta
    17761 agtacaccag ccgattcttc aataatttga cgtctatcaa ttggtttagc atttagtatt
    17821 tcatcaactc taccttgcga gataatgcta tatgcttctt tccccaatcc agaatctaaa
    17881 aataaatcgg caatatcttt taatcttgca cggtcattat ttatgtagta ctcactttca
    17941 ccacttcgat acaatcttct tgttacaata acttcgtttt catcaacact gagcttttta
    18001 gaatgattat ctaatcttaa ctgtacttca gcataatttt gagctttgcg atgttctgca
    18061 cctgagaaga taatatcttc catttttgag ccacgtaatg atttagccga ttgttcaccc
    18121 aacacccatt taatagcatc tgtaatatta cttttaccgc ttccatttgg accaacaatt
    18181 gcagtcacac ctttatcgaa ttgaacattg gtttgatctg caaaagactt aaatccaatg
    18241 gcatctattg attttaaata aaccatccta aactccttat cacaatttta aagaacacat
    18301 gtatacgata ttttagtatt tgtatattaa atttcgaagt tgttccataa aatgttaaaa
    18361 attcttccat aaatcctaaa tcgaacacct atatgtcgta tcatataaat ttctatttaa
    18421 tttgctttaa ttgcttatag gcactttcag cagcgcgttg ttctgattct tttttcgttt
    18481 tccctttacc ttcagctatt gcttcccctt gcagaataac ttctgaagtg aatagacgat
    18541 gatgtgccgg tccctcttct tttattaaat tataggttac atcaccttta ttttgctggt
    18601 gcacatattc ttggaattgt gttttaaaat ctaccacgcc taataactca ttttgttcta
    18661 catgtgggaa aatgactttc tcagcaaatt tccaaactat atctagtcct tgatccaaat
    18721 acaatgcccc aataaatgct tcgaatgcgt ctgatattaa tgatggtctt gtacgtcccc
    18781 ctgttttctc ttcaccttta ccaagtaaaa tcatttcgtt caatccaatt ttattcgcaa
    18841 atattacaag tgagggctca catacaatag tggcacgcat ttttgttaaa ttcccttctg
    18901 gcaagttggg atgtttatca aataaatatc gtgaaaccgt caattctaat accgcatcac
    18961 ccaaaaactc taaacgctca ttatggtcta aacgattcat attaaaatca ttaataaaac
    19021 tcgaatgcga aaatgcttgt tggtataaat caatattttg ataagtaaag cctaactctg
    19081 tcattttagt atcaaagcgc tttctaaaac gattaactat ctcacttttc ttttgtttag
    19141 acattatttt atctccttta tgtgttgctc ttgaatcatc tttaatttta cgttaaaact
    19201 attgataatt aaagaaaaaa actgagtcga caatactgac gacccagatg taagatttat
    19261 tatttttcaa gactgttaat aaatttaaca gcatcaccaa cagtgttgat tttttcagct
    19321 tcttcatcag gaatttcagt accaaactcg tcttctaatt ccattactaa ttcagcgata
    19381 tcaagtgagt cagcgcctaa atcatctttg aaagatgcat cttcagttac tttatcagcg
    19441 tctacaccta aacggtcaac gatgatatct tttactttat cgaaattttc cacgtcgatt
    19501 cacctccttt aaaaatgcct atacatagac tattatattt tcccatttta aattctaaaa
    19561 aacaagtaga aattttagaa acagttatca atttattgat tactgtttct tagggagaaa
    19621 ttctggaacc atatcttggt tgcttagaat ttcccccgca aagttgacta ggtcaggcat
    19681 ttctccattg gatgaccagt caaccactgc gtcaatgagc tttagctcaa atatattaca
    19741 tgtacattcc accatttaca tggattgttt gacctgtaat atattttgct ttgtctgatg
    19801 ctaagaacgc tactgtatta gcaatatctg tgtcttgacc aaaacgtgct aacggaattt
    19861 gagtcaacat ttgttcttta agctcatcac ttaaagcatc tgtcatatca gaaacaataa
    19921 aaccaggtgc aactgcattt acagtgatac cacgagatgc taattcacgc gccgcagatt
    19981 tagttaaacc aataacacct gcttttgttg caacatagtt tgcttgtccc ggattaccta
    20041 ctgctccaac aacacttgat aaattaatga tagcaccact acgttgtctt aacatttgtg
    20101 gtgttgcttt ttgaatacag ttaaatacac cttttaagtt tgtgtcaata acatcatccc
    20161 actcttgttc tttcatacgc attaataaat tatcgcgagt aatacctgca ttatttacta
    20221 aaacatctaa agaaccaaat tggctaacta cttctttaat cattgcttta acttcatcag
    20281 catcggcaac atttgcttga atcgcaaaac tgtcaacacc tttagctttg atttcttcga
    20341 ctactgcttc agctttctct ttgctgcctg catagtttac tgctacatta tatccttctt
    20401 ctgctaattg taacgcaata ctacgtccaa ttcctcttga tgcacctgtt actaaagcac
    20461 tcttagtcat tttcattcca tcctttcaca tcttctaaag tttgaattga tgttaactta
    20521 acatctctat ttattttttt aattaagcca gataaaactt ttccaggacc aatttcaata
    20581 aaatgatcaa caccttggtc tattagccat tctgttgagt taatgaattg tactggtgaa
    20641 tataattgtt tgaccatatt agatttaatt acttctttgt cagtttcacc ttgcgcattt
    20701 acattttgaa ctacaggaaa cttagcatca cgccattcaa attgattaat ataacttgaa
    20761 aaatcttctt caatcacttt cattagcgat gaatggaatg ggccagatac tgctaaaggc
    20821 atgacacgct ttgcacctaa tgatttacct ttttctacta gctcatcaat taaagctttg
    20881 tgacctgaaa caacaatttg acctgggcaa ttaatgtttg ctggttcaat tattttgtca
    20941 tcagatgata atgacttaca aatttcatca actttatcaa aatctaagcc caatactgca
    21001 gccatgcttc ctacaccaga aggaaatgct tgcgccatta attgaccacg ttttctaaca
    21061 attttaactg catcttcaaa tgataatacg tcagctgcaa ctaaacttga atattcacct
    21121 aaactatgcc ccatagtaaa atcgggattc aaatttttta gtgctgctaa taatgccgaa
    21181 ctatgcgtca ataaagctgg ttgcgtgttt tcagtttcac ccaatttacc ttcttcgtca
    21241 gtaaacattg tctctaaaat atcaaagtct aatgtgtttg ctgctgaagt taaaatttca
    21301 gttgcttgat cgttgttgtt atataaatct tgtgccatac caactttttg ggcaccttgt
    21361 cccggaaaaa taattgctgt tttactcatt tgattcacct acagtctctt tcattgtttg
    21421 tacaatattt tgctctcctg ctatttttgc ttgtctaatt gcagaataaa aagctttagc
    21481 atttgaacta ccgtgtgctt taactacggt accttccaat cctaataata cggaaccacc
    21541 gtattctgag taatccatct ttttagcgaa ttctgctaaa tctttcttca atattgcacc
    21601 tgctatttta tttttagtac tactcataat cgtatctttt aacattttac cgattgattt
    21661 tgcagtacct tctaaatttt taaggaccat gttcccagta tagccatcgg taactacaac
    21721 atctgtatcg ccatccatta atgtcttcgc ttcaatattc ccaacaaaat tcaatgaatg
    21781 atcttgattt aataactcat atgatttttt cgttaaacta ttacctttag ctggctcggt
    21841 acctatattt aataatgaga ttttcggatt atcaatacct ctaatttttt gagcataaat
    21901 atcccctagt tgcgcatact gtaataagtg ttcaggctta gcatcagcat ttgcaccaac
    21961 gtctaaaaag acaaaacctt ttccatcaat cgttggcaat gttactacta aagccggtct
    22021 agctacacct ttaatacgtc caacaatgaa taaaccagct gacattaaag caccagtatt
    22081 acctgctgac acacatccat ctgcctcacc agatttcaca gcttcagcca tttttaccat
    22141 tgagctatct tttttacgtt taatcgctct aacaggctca tcttccattt caatcttttc
    22201 agaacaatgt ctaaattcga ttcgttcatg gttcagatta tacttttttt cgtcaccgaa
    22261 aagtataatt tctagatctt taaagtcttc aacagccttt tgtacggctt ctaatacgat
    22321 atcaggcgca ttgtcgccac ccatcatatc aattgctaat ttaaccataa ttttatcctc
    22381 gcttatcata aaacatttta aaatttcctt tgaaaactaa tgtatgttta acatatgact
    22441 ttacttcgac gtaataatgt tttgcagttt gatttacaac tcgtgcttct gctcttaccg
    22501 tatcatttaa ttttactttt tcaataaatt gaatgctact ctcatgagtt aaaactgttg
    22561 gttgcttaat tagcgcaaca cataatgaat ttgcctgagc aaacagcaca tgaccacgcg
    22621 caattccagt tttatgaaaa acagaatccg atgtaatatc taaaattgat tgcgctttaa
    22681 catttggatt gacttgaatc aaatcaccaa taaattcttg ttcttcaata gaacttattt
    22741 ggtcataatt tttttcagca actaatttaa tacgcttcct taattctggt atgtttaaat
    22801 aagtgcgatc taaacgaatt gtttgtatac tcacttgaaa taagtcgctt agttcatggt
    22861 ctgtgatgaa gggattgcta tcaatttgtt gtctgattgc ttctctacgt ttatctttct
    22921 ttagtttcaa cgtctcaccc ctcatttttt agtacctagt cttaaacatt cctcacatat
    22981 cataacactt tctatttaac cgactcaaca aataactgta ttatttagct attatttagt
    23041 cgaaaattta taaattttta aaataccact ttacatttaa atagttcaag tgtcatatca
    23101 ctcaattaac gaagtgttgc acttaaattg acaaatcagc atggtaatta gtcaaaactg
    23161 cgatgtaata aattttcttc aacaaaatga cgtaaatgtt gatacgtatt ttcaaagaat
    23221 acgccagatt gaataagttc agctgcttca tcacgagcaa cttctaacat acgataatct
    23281 tcaactaaat tggcaactaa gaaatctggc aatccacttt gtttaacacc aaagaaatct
    23341 ccagggccac gcatttctaa gtctcgttca ctcaattcaa atccatccgt tgtttgtgtc
    23401 ataattgtca ttctttcaat tcctgtttct gttttagggg atgcaattaa aacacagtaa
    23461 ctttgctggt cacttctacc cacacgaccg cgtaactgat gtaaagttga taatccaaag
    23521 cgatccgcat cataaatcat cataaaagtt gcattcggta cattaacacc tacttcaaca
    23581 acagtagtag aaactaaaac atctatctca tgattgctaa acttttgcat gacctcatct
    23641 ttttcatcgg cagacaactt accatgcaat aaccctacac gggaaacacc ataatactgt
    23701 tgtaaagact cgtacaatgc gacaacattt tgaacatctt cgagatgctc agaactttct
    23761 ataagcgggc aaataacata tgcttgacga ccttttttca actctgaggt catttgcatc
    23821 aaaactttat cgtattgctc atgctttgcc caagtagtaa tgataggttt acgacctttt
    23881 ggtaattgtt taattgaaga cacatccatc tcaccaaaaa ctgatattgc tagtgttctt
    23941 ggtatcgggg ttgctgtcat aaataacaca ttcgtcattg cacctttttc tcttaaaagt
    24001 tggcgttgat tcacaccaaa tcgatgttgt tcatctgtaa ttactaaacc aacattatgg
    24061 aaaatcacat catcttgaat caatgcatgg gttccaatta aacaatcaat cgtacgattt
    24121 tcaagttgtt ctaaaagtat ttttcgtttc ttacctttta ctgacccagt taacaacgca
    24181 acattcatag aatctccaaa taaagcaatt aaactttcag catgttgctc tgctaaaatt
    24241 tcagttggta ccatcaatgc tgattgataa ccagccgttt ttaacgcata catacaaatt
    24301 gcagcaacta ctgtttttcc tgaacctaca tcaccttgaa gtaatcgatg catacgtatt
    24361 ggtgctttta aatctctaaa aatttcatta acactggatt tctgtgcttc agttagttca
    24421 aaaggcaaac gatcaataaa tgatttaact tggtctaagt cataatcaat ttcaattgct
    24481 tcgtcagatg acttttctaa tctatttagc cattgcatac gtaattcgaa taaaaacagt
    24541 tcagtaaatg cataggttct acgagcacgt aataaatcct ctttactttt aggatgatgt
    24601 aacgtgttca aagtaaagtc caaggtctct aatttatatt tttctcttag ttcatcagtt
    24661 aaccattcat gaattgtcac atcatttaac gcttgtctaa tttggtctcg tatttgcttt
    24721 tgtttaatac cttccttaat acgatagact ggttctaatt gaacatctgc gttttcttga
    24781 gtttgtgtac cttgtgaatt caagaaaacc ctattaccag taatttcctg tttaacccta
    24841 ttccacttac ctttaacagt tatagtttga tttaattcga ttttcttttt taaatacggt
    24901 tgattgaaaa aaatacattt gacagcaata ttatttacca ttaaatgaac agttaatttt
    24961 gatttatttc ttccaaaaaa tgcaactact ggagctgtat atacttgtcc ttctatcgta
    25021 acgttagatt gatcttctgc ttgattcaaa tcaatcactg tattatcttc atatctagtt
    25081 ggcaaataaa gaacaagatc ttccactgta tgaatattta gttgttgcaa tacttctatt
    25141 ttcttaggac ctataccttt taattgtaaa agagaatatg gactttctat taagtttact
    25201 ttagccaaaa taaatcacct atgttttatt tgattttacg tatcatttag tatgttattt
    25261 attttatcat gactaagttg aacatgacat tgttagcaac tcgagaacga tacttttaac
    25321 ccgcaatatc actaaaatag caaaataaaa aactaccaac tgatgattta tcattggtag
    25381 ttttttattt taaattttta ttctactgaa aagaaatatt gataaattgg ttgtccacct
    25441 tcatgaactt ccacttctac atctggatat tgctcttcaa tccagtttat catgttatct
    25501 gtaactgctt gctctgcatc ttgaccaata atcacagtca atatttcact atcatctgct
    25561 aacatctcat ttaacaactc agtaaccgtt gttaattgat cactttggct gcttacaatc
    25621 ttatcttcaa tcaagcccat aaacgcgtct tttttaatct caacgccatc aattttcgta
    25681 tcacgaacag cgtacgttaa tgaaccagat ttaacgttat ttactgaatc agccatttgc
    25741 gctttatttt cttcaagtgt tgcgtccaca tcatattgga atagtgcgct tataccttga
    25801 ggaatagatt tcgttggaat aacaactgct tctgcatcaa caatactcgc tgcttgttca
    25861 cttgccatta agatattttt attattcggt aaaataattg cacgtttaca ttttgattgt
    25921 tcaatgactt taacgatatc ttctgtagaa ggattcatcg tttgtccacc actaatgata
    25981 tgtgtggcac ccattgattt aaatatctct gaaatacctt cacccataga aatagtaata
    26041 attgctgttt caaccgtttc cattttcggt ttagctgtgt gctgttcttt tcgaatcact
    26101 tcacgatgct gttctctcat attttcaacc ttaagtttaa ttaattcacc atattgttga
    26161 ccataattaa acactttacc tgggtattcg gtatgcacgt gaactttcac aatttcttca
    26221 tcattaatga ctaataaaga atcaccaaat tgactcatat cttgcctgaa ttcttgttca
    26281 tcaaaggctt ttttattctt tccaaaacga accatcattt cagtacaata gccataaata
    26341 atatcttcag tattaattac accatggaaa tcatgttcat catgtacaaa ttcatcttta
    26401 tctagctttg caactttggc ttcaactttt tcacctttaa gcgcttttaa gaatccttcg
    26461 taaacgcata acaaaccttt accgccacta tcaacaacac caacttcttt aagtacagct
    26521 aataagtttg gtgtgttttc aagtgattca ttggctttta caataatgta ctccattaat
    26581 tctatacaat cttcagtatt atttgctttt tctattgcag cttgcgcagc atcttttgca
    26641 actgtaagta ttgtaccttc aactggtttc ataacagctt tatatgccgt ttcaacacca
    26701 gcttgaaaac tttcagctaa caattttgaa ttaatttcag attcactttc aatattttta
    26761 caaaatcctc tgaataattg tgacaagatg acaccagagt tacctcttgc acccattagt
    26821 aaacctttcg agaatgtttt acctaattcg ccgatatttt tcgacaaatt attctctact
    26881 tcttcgcgac ctgaagtcat agtaagattc atatttgttc ctgtatcacc atctggcact
    26941 ggatacacat tcaaagaatc taccaaatct gcattgttag ataaattttg tgccccttgt
    27001 ataatcatat cggcaaataa tttaccatta attttgctaa tcatttcaag ttgtcctcct
    27061 aagctttctt gcctgtatta ttcacacgta caccttgtac atatatattg attgaattta
    27121 ctgatacatt aagtgatttt tccaaagtat atttcactgt tgattgtaca ttattggcaa
    27181 cttcagatat tttcacaccg taacttacaa taatgtacat atctatatcc actacgccat
    27241 tattttcagt tactttgatg cctttagcat aattttcatg tcctagtatt tccgcaatac
    27301 catctctaac ttgttgtcta gatgccatac ctacaatacc ataacattca acggcctttc
    27361 cacctacaac cgaagcaatc acttcgtttg aaatatcaat tttaccgtaa tcatttgaaa
    27421 tctctaatgt catatctttg tccctcctaa atttcaaagt ttattattcg tttcccctaa
    27481 tcacttttaa aattttacca cttagtgtag tcataaacgc tttaagtttc attgattatt
    27541 tttataaaat atcaatggta caacttatca gaatgactta cgcttaaatc acctattata
    27601 ttttgagtaa actgaattta cactcatgac gattatgtat aggcattttc ttttaataga
    27661 tgtagtatca ttaaataaaa agtttaaatg tttcattcac ttatttctct aaaatatcat
    27721 actacataac ttcacagtct acaattatat cacaaaactc agtatttttg acttttatgt
    27781 gaaaat
//
LOCUS       DAKSQE010000023        27662 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.023, whole genome shotgun sequence.
ACCESSION   DAKSQE010000023 DAKSQE010000000
VERSION     DAKSQE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 27662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27662
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.023"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(129..311)
                     /locus_tag="O0V33_000858"
     CDS             complement(129..311)
                     /locus_tag="O0V33_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975522.1"
                     /translation="MAQRYIKVLILYIFTTLLSSITVTSKCVPNKVVRFILRTVVGYG
                     IFAYGLHYFSNLKKNK"
     gene            complement(397..864)
                     /locus_tag="O0V33_000859"
     CDS             complement(397..864)
                     /locus_tag="O0V33_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCX8975523.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKALFEKWFHPKDAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSEFNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(1050..2597)
                     /gene="adcA"
                     /locus_tag="O0V33_000860"
     CDS             complement(1050..2597)
                     /gene="adcA"
                     /locus_tag="O0V33_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HCX8975524.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHGKEEHHHHGGYDPHVWLDPKI
                     NQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISHE
                     SIGYLADRYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTETI
                     RKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAESK
                     HDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKDL
                     KAYYDKGYKTDITNIDIKGNEITFTKDGTKHTGKYEYNGKKTLKYPKGNRGVRFMFKL
                     VDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQI
                     KEEMLAH"
     gene            complement(2890..3156)
                     /locus_tag="O0V33_000861"
     CDS             complement(2890..3156)
                     /locus_tag="O0V33_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCX8975525.1"
                     /translation="MVRLNITFSPQAFEDYKYFQQNDKKMVKKINELIKSIDRNGALK
                     GIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIKIASCKYHY"
     gene            complement(3156..3407)
                     /locus_tag="O0V33_000862"
     CDS             complement(3156..3407)
                     /locus_tag="O0V33_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCX8975526.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSDIVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIADLERGKTVTKDIDV"
     gene            complement(3678..4277)
                     /locus_tag="O0V33_000863"
     CDS             complement(3678..4277)
                     /locus_tag="O0V33_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCX8975527.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVIYGDYKC
                     PYCKELDEKVMPKFRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(4296..4658)
                     /locus_tag="O0V33_000864"
     CDS             complement(4296..4658)
                     /locus_tag="O0V33_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCX8975528.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDDEVHYYAYDFSDKRVSYKQDFDS
                     RRYYQQHDADYHEENMTN"
     gene            4913..6163
                     /gene="fmhA"
                     /locus_tag="O0V33_000865"
     CDS             4913..6163
                     /gene="fmhA"
                     /locus_tag="O0V33_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HCX8975529.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKQNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDTLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDFSESAEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPILYKLQTYLKHKRR"
     gene            complement(6260..6991)
                     /locus_tag="O0V33_000866"
     CDS             complement(6260..6991)
                     /locus_tag="O0V33_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCX8975530.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVGKGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVFVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKAEAREKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAREVSNNIVFIHEGVIGEQ
                     GAPEEIFNNPKTEELKRFLNVINEE"
     gene            complement(6988..7707)
                     /locus_tag="O0V33_000867"
     CDS             complement(6988..7707)
                     /locus_tag="O0V33_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCX8975531.1"
                     /translation="MFLNLNSEQLHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKVLRSIARVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYRQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(7688..8467)
                     /locus_tag="O0V33_000868"
     CDS             complement(7688..8467)
                     /locus_tag="O0V33_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCX8975532.1"
                     /translation="MKRLLFVVIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKAEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFNDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSKSAFAF
                     SKKVDDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(8588..10027)
                     /gene="mdeA"
                     /locus_tag="O0V33_000869"
     CDS             complement(8588..10027)
                     /gene="mdeA"
                     /locus_tag="O0V33_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HCX8975533.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLVSMIGGIVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIEMMNQIKIDTMLHGYHAG
                     FLFALLITVISFFCSFMLQGKKKEVDSHQ"
     gene            10200..10304
                     /locus_tag="O0V33_000870"
     CDS             10200..10304
                     /locus_tag="O0V33_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCX8975534.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(10457..11143)
                     /locus_tag="O0V33_000871"
     CDS             complement(10457..11143)
                     /locus_tag="O0V33_000871"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCX8975535.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIDIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
     gene            complement(11470..12336)
                     /locus_tag="O0V33_000872"
     CDS             complement(11470..12336)
                     /locus_tag="O0V33_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCX8975536.1"
                     /translation="MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGI
                     SSVYVHPEPDHL"
     gene            complement(12836..12940)
                     /locus_tag="O0V33_000873"
     CDS             complement(12836..12940)
                     /locus_tag="O0V33_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975537.1"
                     /translation="MLTNRLTSVNYVKKRLIQNIQRILSKKVLYGEMQ"
     gene            13035..14348
                     /locus_tag="O0V33_000874"
     CDS             13035..14348
                     /locus_tag="O0V33_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein sbi"
                     /protein_id="HCX8975538.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     SKLNEKDSIENRRLAQREVNKAPMDVQKHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKAESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYYNKYYQYKGLIDKTVLTTIGSGYGSYIKPLEVSKESGNLAKSYAQVRNYVT
                     ESINTGKVLYAFYQKPELVKTAIKAQETATTFKNALTGIFKSFWK"
     gene            14884..15813
                     /gene="hlgA"
                     /locus_tag="O0V33_000875"
     CDS             14884..15813
                     /gene="hlgA"
                     /locus_tag="O0V33_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HCX8975539.1"
                     /translation="MIKNKILTATLAVGLIAPLANPFIEISKAENKIEDIGQGAEIIK
                     RTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMI
                     WPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNY
                     SKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARD
                     YFVPDNQLPPLIQSGFNPSFITTLSHEKGKGDKSEFEITYGRNMDATYAYVTRHRLAV
                     DRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK"
     gene            16363..17310
                     /gene="hlgC"
                     /locus_tag="O0V33_000876"
     CDS             16363..17310
                     /gene="hlgC"
                     /locus_tag="O0V33_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HCX8975540.1"
                     /translation="MLKNKILATTLSVSLLAPLANPLLENAKAANDTEDIGKGNDVEI
                     IKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKNTNHIKS
                     MRWPFQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSF
                     NYSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKD
                     PRDYFVPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTH
                     YGNSYLDGHRVHNAFKNRNYTVKYEVNWKTHEIKVKGQN"
     gene            17312..18289
                     /gene="hlgB"
                     /locus_tag="O0V33_000877"
     CDS             17312..18289
                     /gene="hlgB"
                     /locus_tag="O0V33_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HCX8975541.1"
                     /translation="MNMNKLVKSSVATSMALLLLSNTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKAAGNINSGYERPNPKDYDFSKI
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK"
     gene            complement(18341..18808)
                     /locus_tag="O0V33_000878"
     CDS             complement(18341..18808)
                     /locus_tag="O0V33_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCX8975542.1"
                     /translation="MTSKDITQISVIAAILTILAVLKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSLLLLVFGVFNPINVIISIIFRVIAIAVVYLLKINVLSLVLAS
                     VLGSLVSRLILSVILNLPVWVVLLNAIPGVIFTLIVAIPLYLILRKRMAVLLR"
     gene            complement(18819..19511)
                     /locus_tag="O0V33_000879"
     CDS             complement(18819..19511)
                     /locus_tag="O0V33_000879"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HCX8975543.1"
                     /translation="MYSIKMRSSNQDIHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIKQIKALQIIEDDKANLQHLTQKCGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGHRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFASAKIGYHRLFDIKPVNTRYGGRIIFVDDRIDLNHYILFL
                     ESTPKQVVYETV"
     gene            complement(19522..20637)
                     /locus_tag="O0V33_000880"
     CDS             complement(19522..20637)
                     /locus_tag="O0V33_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HCX8975544.1"
                     /translation="MMFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFESFMRKYSYHLSSSRLISGSSTVYEEIESMLAGWLGYSACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLAE
                     YPNQNIQKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGI
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFT
                     SYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED"
     gene            complement(20615..21622)
                     /gene="bioB"
                     /locus_tag="O0V33_000881"
     CDS             complement(20615..21622)
                     /gene="bioB"
                     /locus_tag="O0V33_000881"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCX8975545.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSREIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNDVSRA
                     N"
     gene            complement(21624..22982)
                     /gene="bioA"
                     /locus_tag="O0V33_000882"
     CDS             complement(21624..22982)
                     /gene="bioA"
                     /locus_tag="O0V33_000882"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCX8975546.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSQKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKCKLLSQIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            complement(22960..23646)
                     /gene="bioD"
                     /locus_tag="O0V33_000883"
     CDS             complement(22960..23646)
                     /gene="bioD"
                     /locus_tag="O0V33_000883"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HCX8975547.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCIFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMANQIFLNKQCVLDKVKVLDK
                     EFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDY
                     VNQNVSASNFLIMNRYTGSYIEKDNQITIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
     gene            complement(23701..23868)
                     /locus_tag="O0V33_000884"
                     /pseudo
     CDS             complement(23701..23868)
                     /locus_tag="O0V33_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24099..25832)
                     /locus_tag="O0V33_000885"
     CDS             complement(24099..25832)
                     /locus_tag="O0V33_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8975548.1"
                     /translation="MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLVAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDVVVNGYILITVLILSLLVVSWQ
                     VSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQAVNGSKRINTKIEMQFIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLP
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHLLLRFYDIDDGNIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGDPGATKEEIIRAAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFI
                     RIKSKSAGWKL"
     gene            complement(25857..27620)
                     /locus_tag="O0V33_000886"
     CDS             complement(25857..27620)
                     /locus_tag="O0V33_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8975549.1"
                     /translation="MNNQTNWIKILSGFASDSKWKIMLSILLSIISVFSGLVPYWAVF
                     KIILMMINNAYTINSIMVYIFIVLIAYISQVCCFGASTMLSHITAYEILSEIRKKLAQ
                     KLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIMLID
                     WRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKTFNQ
                     SQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLISINQLN
                     YAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLSTKFKKPQH
                     YEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFIAIVGASGSGKSTIAKLISRYWDVT
                     SGEITIGGIDIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEKAAK
                     LAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEATAYVDP
                     DNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKLNGN
                     YKKMWDTHMHTKDWGINTGHN"
BASE COUNT         9415 a         4994 c         3762 g         9491 t
ORIGIN      
        1 attaaaattc agaaaattct ttttaccaat tcaaaactaa tatttactca taaaaatagc
       61 gagatagata tcgcgacata aaacaattgg tatcatttta attgcgctca tctcgcttta
      121 tgtaagtatt acttattctt ttttaaattt gaaaagtaat gtaaaccata tgcaaatata
      181 ccgtagccta cgactgtacg caaaataaat ctaacaactt tatttggtac acatttcgaa
      241 gtaacagtaa ttgaagataa aagcgtagta aaaatatata atattaatac tttaatgtaa
      301 cgttgtgcca tcgaatcacc cttcattcaa ttaataatat tacgttatta ttataaactg
      361 aatggcatat aaaattttta catattttca acaactttat ttcaataaag catcaagttg
      421 atccaatgtt tgattcatcc cttgctcaac acccatgttt atgacttgtt gagcggcttc
      481 tttagttgga aataccgatg ttgatgtcac tgtcgttttc gttgtattga actcggaaaa
      541 tgacaaagta atcttcatgc ttggcatttt tgtatctttt gcaccttgag gtgtcgcaaa
      601 cgagtcaata tattcaatta aatatggacg cttaaccgtt ttatattctg ctaatgtata
      661 gcttgtcatt gtgggtgttt taatcgcgta aaatgcatca ccgcctgaaa cagcattaaa
      721 gcgaaacact ttggtactag cgtcttttgg atgaaaccat ttttcaaata atgcctttgt
      781 cgtataggca tcaaacacct tttcaatcgg tgcttctatc gttcttgaaa aaataatttt
      841 attatcttca actttaatcg tcatcttccc actccctatc ttttatattt actcatctta
      901 acatgcattg agtgacatat gttaccaaat catttgttat aaactatagt aatattaatc
      961 attccctctg ttgaaattga taactacgaa ataaaaaaca cactctattc agctattaac
     1021 agttgagtag agtgtgcaaa cctgttgaat taatgcgcta acatttcttc tttgatttgg
     1081 tctttattta attttgaagg ataatatgtt ggccagttat ccatttcttt taataacgcg
     1141 tcattatcat tacccataaa gatatggaag tgttctgcct ttttaggtgc aatgttgtga
     1201 tcgctaaatt ggatgaattt cggtaagtct ttatcattac catcgaccaa tttaaacata
     1261 aatctcacgc cacggttacc tttaggatat ttcaatgttt tcttaccatt gtattcatat
     1321 ttaccagtgt gtttcgtacc atctttagta aatgtaattt catttccttt tatatcaatg
     1381 ttagtaatat cagttttata tcctttgtca taataagctt ttaaatcttt agcagatttc
     1441 ttcggatcat tttcagcttt atgttccatc acttcatcaa gcgtaccgtc ttttaagtaa
     1501 gggtaaacag attgccattc accagcataa tcgcttaatt cacggtcttt aacttgctca
     1561 tctttaaaat acccatcaga aattgctttg tcgtgtttac tttcagcttt gtcgtctttc
     1621 actttaacac cactgtctaa agctttaccg atattttcaa tattcgattt cattaatgat
     1681 tgataggtaa tattatcttt tttctgttgt tctttattta aagactccat gttgtagaat
     1741 tttaaaggct tcgcatctgt ttctttacga attgtttcag tcactttatt cgcaacatta
     1801 tcttcgtaaa gaatatattt agcattgcta tctctaattt ctttaacaat tttagtcaat
     1861 tctttttgtg atggatcttc agcattcatg ttttgaatac ctttttgaac aaaaccataa
     1921 cgatcagcta agtatccaat tgattcatgt gaaatgaata ctgcattacc ttgcttatct
     1981 ttagtaactt gcttcatatc gttatcaatt ttcttaagat cgtcgtttaa ttttttgtag
     2041 tttttctcat agtcatcttt atgttttgga tctttcttca ctaattcatc tttaatttct
     2101 ttagcgaaag tttggttaat tttaggatct aaccatacgt gtggatcata tccgccatga
     2161 tgatgatgtt cttctttccc atgatcatgt ccctcatgtt catgctcttc accatgttcg
     2221 tgttgatcag ttaaaagctt tgctttatct aatttatctt ctaaagacag ttttttatct
     2281 ttatctttaa tagtagatgc aactttctta gcaaccggat ctaaattatc ccctgtatac
     2341 ataaacaagt ctgacttgct tgcacttaat atatcttttt gtgttggttc atagctatgt
     2401 aaatctgtcc ctgctggata gattgatgat accttcacgt gttttccacc aatttgctct
     2461 gcaaatgatt gcaatggata aactgtcgtt ttaattgtta ccttgccatc tttatctttt
     2521 ccatcatcat tcccacatgc ggctaatgat aaagttacgg ctggtacaag aagtaacata
     2581 cctaatttct ttttcatagt caccctccta aatagtaatc tttacgtttt aaaattatat
     2641 actcatgcta cttaaaatac aagcactgtt tttataaata atataaagtc gaatcatact
     2701 ttaggtattt accacttttc gttttatcta atacatgact attgtacaaa tatgatacag
     2761 aaaactttat agtatttacg aacgtcgtaa tataagaaaa aagacaatct ctatcatatt
     2821 gctaatagaa attgcctttc atattgtcct aaaataactt accctatttt tatagtgtga
     2881 cttttagatt taataatggt atttacatga tgctattttt atatgattgt catctactgt
     2941 ataaaccaat ctatgttcgt gattgatacg tctactataa tacccagtca gattcgattt
     3001 taacttttca ggcttaccta tacctttcaa cgcaccattt ctgtcaatac ttttaattaa
     3061 ctcattaatc ttcttcacca tttttttatc gttctgctga aaatacttat aatcttcaaa
     3121 ggcttgaggc gaaaacgtaa tatttaacct aaccattata catctatatc tttcgttaca
     3181 gttttcccac gttctagatc tgcaattgat tgagctaagt gttcagcatt atttggattc
     3241 tgttggaggt aaagtgtttc catcatggag ttataatctg attctgacat gattactacg
     3301 tttttatcat cagtagatgt tacagttact atatcactat catcatttac ttttgtcata
     3361 agtgccttta aattctgtcg agcgtatgaa taatttttaa taatcatatt tatcgcctcc
     3421 ttttaacaca ccccaattgt acaaatattt gtacaataaa acaagagaca cagaaaaaac
     3481 cactaatcaa gattttgcac ggggaagaat caatacctat cctgtactga ttccctacta
     3541 aacaatgttt acatgataat ttccacctat ttagacttca tacattaaaa agacaatctc
     3601 tatcatattt ctaatagaaa ttgcctttca tattgtccta ataaattggt atcacttact
     3661 tatctcaaca attaagacta tttgatttta tcttttaata atttttcata actttcataa
     3721 tcatatggat cttcaacttt ctcgccatta ataaatgcag ttggtgtcgt ttttatatga
     3781 ttatctttag ctattttttt atctttctct gcagctttcc aagacttgct atcttttgtc
     3841 ttgtaatctt ttataatttt attttccgtc tctttatcta aatgcagttg tttaatatgt
     3901 ttatctaata gttcttttgt taaccattct ttattttcat cttgctgggc agcaaataat
     3961 tgcttttgaa aatctaaaaa tgatttaggt gcatacatca atactgcatg actcgcacgt
     4021 gaaccaacaa ttgagtcttt acctaagaaa gctaaattga caaattggta ttccactttg
     4081 tgattatcta tataattttt acgaaacttt ggcatgactt tttcatctaa ttctttacaa
     4141 taaggacatt tatagtcgcc atatatgaca actaatggtt tgccgttttt cgaagatgtc
     4201 gttgctgatt cttttttacc gcaagctgtt aaaattaaca tgctcactat aaataatgtt
     4261 agtaattttt tagtcataat aactcctgtt catatctagt tcgtcatatt ttcttcatga
     4321 taatccgcat catgttgttg ataatatcgt ctcgaatcaa aatcttgctt atatgataca
     4381 cgtttatcac taaaatcata tgcataataa tgcacttcat catcattact caaaggttta
     4441 taagctaaaa tgataatttt atccttatca tagacataaa tattagcatc tttgcgttca
     4501 aaatgatcaa cacgatcacc gttgcctttt ttggcaagtt gttcttgttt ttgattttga
     4561 attttaactg cttcatctat ttctttcaca tatttatcat taccacatcc tgatataaag
     4621 actagtaata ataataccgt cgccacaaat cgcttcaatg ctcccactcc tactatcaac
     4681 ttccaactaa ctttcaatat aattaatact atacaccgtc aaacatcaat gcgctacaac
     4741 tactaccaat acatttaata taaatatcca aagaaataac agttatgtat agtcaaagaa
     4801 aaatatattt cattttctat gtgtatttgc aatcagtttt tgataaaata aaaaaatagc
     4861 attatatata aattcaaaaa ttacagttca attcagagag ggatgggata aaatgaactt
     4921 tgtaacgttg acttcggatg agttcaatgc gtttacaaca aagcattttt cacattacac
     4981 acaatcagct attcattaca atcatagagt tgatttaaaa ggcgatgtgc atcttgtagg
     5041 ggttaaagat gacaatggtc aagtgattgc aggatgctta ttgacagaag cacgcacact
     5101 taaatttttc aaatattttt atactcatcg cgggccagtg atggattata caaatcaatc
     5161 attagtagca tttttcttta aagcattaac gtcatattta aagaaacaaa attgtttata
     5221 tgtccttgta gatccatatt taattgaaaa tttacgcaat gcagacggtg aaattgttaa
     5281 gtcttatgac aaccgagcat ttgttagaac aatggacaca ttaggctata agcatcaagg
     5341 ctttccagtc ggttatgatt caatgagtca aatccgttgg ttatctgtat tagatttaaa
     5401 ggataaaaca gaagaccagt tattaaaaga aatggattat caaacgagac gtaatattaa
     5461 aaaaacatat gatattggtg tcaaaactaa aacgttaacg attgatgaaa cgcaaacctt
     5521 tttcgactta ttccatatgg ccgaagaaaa gcatggtttc aaattccgtg agttaccata
     5581 ctttgaagaa atgcaaaagt tatacgatga ccacgccatg ttaaagttgg cgtatattga
     5641 tttaaacgag tatttaaaaa cgttacaatt aaagcaacaa caattaacag ctgaactctc
     5701 aggtgttgaa gaggcattgg aagaaagtcc taattcaaag aaaaataaaa cgaaacgcac
     5761 acagctagaa caacagctga atagcaataa gcgtaaaatc gataatacaa tagaacaaat
     5821 tgaacaagat ggtgcagtgt taaacttagc ttctgctttg tttatctaca atgaacatga
     5881 agtttattac ttatctagtg gttcaaatcc taaatataat gcttatatgg gtgcctacca
     5941 tttgcagtgg gaaatgatta agtttgctaa agcgcatcat attgatcgct ataattttta
     6001 tggcattact ggtgattttt cagagtcagc agaagactat ggcgtacaac aattcaaaaa
     6061 aggatttaac gcacacgtag aagaatatat tggtgatttt attaaaccaa tcaaacctat
     6121 actttacaaa ttacaaacat acttaaaaca taaacgccgt taacagttta tgctgtaaca
     6181 tctagaatat aacaaagaac cacgcatcat gtatatggca tcatcgccat attaaaacat
     6241 gatacgtggt tctatttgtt tattcttcat taataacatt taaaaagcgt tttagttctt
     6301 cagtttttgg attgttaaaa atctcttcag gtgccccttg ttctccaatt acaccttcat
     6361 gaatgaatac aatgttatta gatacttctc tagcaaaacg catttcatgt gtcacaatga
     6421 ccattgtcat gccctcattc gctaaatctt taatcacctt taacacatca ttcacgagtt
     6481 caggatcaag cgctgatgtt ggttcatcaa acaacatcac tttaggattc atagctaatg
     6541 ctctcgcaat ggcaacacgt tgctgttgac cacctgataa tgcatgaggt cgttgatctt
     6601 tgacatgtgt caaaccaact ttctcaagta attctagaga tttctcacga gcttcagcct
     6661 ttttcaactt tttaactgta ataagacctt ccattacatt ttctaatgcc gtcttatgcg
     6721 gaaaaagatt atagctttga aataccatac cagattgttt acgaacttct atttgtgatt
     6781 ttttatcttt agatgtatat gttttgccgt taacaaatac tgtaccttca gttggaattt
     6841 ctaacgcatt aatcattcgt aacaacgttg ttttacctga cccagatcgg ccaattaagg
     6901 ttacaacctc acctttgcca acagataaat caataccttt gatgacttca acatcattaa
     6961 atgatttatg gatattgttc aattgaatca tgagcgatac cctctttcaa tataagattc
     7021 gtagatacct tggattattg aaataataaa gcataccacc cagtacatta gcgcaactaa
     7081 cacataaatt gttaaatatt catatgttgt agacgcaact tcttgagctt ttctaaacat
     7141 ttctgctact aaaataaatc ctagtaatga tgtatctttg attaaactta aaaatgtatt
     7201 acctagtgca ggtatagata ctcgaattgc ctgcggtaag atgatgcgtt gtatcgtttg
     7261 acgatatgtc ataccaatag aatatgcagc ttccgtttgt ccttttggta tagaaataat
     7321 accgccgcga ataatttctg aagcatacgc acctacattt aatgacaagc caataatggc
     7381 agcgaccaca ggcgccaatg tccattgatt atctgcatca ttggttaata atctacctaa
     7441 ttctggaata ccataaaaga tgataaataa ttgtactatc ataggtgtcc cacgaataat
     7501 agatacatag acacgtgcaa tacttcttaa aactttactc gttgaaatgc gcatcaatgc
     7561 tgtaaataat gcaatgatta accctaatac aaatgttacg agggtaatcg gaattgaata
     7621 tttcaccaaa ccttcgagca taggcccaaa agcttgtttt gcagcatcaa gtgcatgtag
     7681 ttgttcacta tttagattta gaaacatctt gaccaaacca tttcttacct attttagcta
     7741 attcaccgtt ttcctcgatt tttttcaagc catcattgaa tttttgaact gtttcgtcat
     7801 ccactttttt agaaaatgca aatgcagatt tactttgttc agcgttacct ttgattgcct
     7861 tgatcttagc attaggtttt tgttttttat aatctaagta tgacagactg tcattaaatg
     7921 taccatcaac acgcttagac aataataaat ccattgattg gttaaagcca tcaacttttg
     7981 taatatcagc acctttatcc ttagctaatt taccataatt agatgtgaat gtttgtgcta
     8041 acttcttacc tttaacatca ttaaaatctt taatatcttt ttcattttca cgaataacta
     8101 aaacagcact tgagaatgtg taaggcttag aaaatttata tttcttttct ctatcaggat
     8161 taatacccac ttggttcgcg attacatcaa aacgccctgc gtctaaacct gcaaacatag
     8221 agtcccaaga agtttcgtta aatttaagct ttaatccttc tgctttagcc actgctttaa
     8281 taacatcgat atcataacca gttaatttgc catctttatc atggaaacta aatggtgcat
     8341 aagtaccctc agtaccaaca ttaattgtct tactatcttt actagcttcc ttgtctttag
     8401 acgaattgtt cccacatgca gctaatatga aaacgaaagc tatcaccaca aataaaagtc
     8461 ttttcataga atatatctcc tcattcttat tattctaatc ggaataaaaa tattttaatc
     8521 tgaatcgcca gataagtcaa tatgttgaac ttttgctaaa aagcgttata tagctagcaa
     8581 cttatattta ttgatgtgaa tcaacttctt ttttctttcc ttgtaacata aatgaacaaa
     8641 agaaactaat cactgtaatc aatagcgcaa ataaaaatcc agcgtgataa ccatgtaaca
     8701 tcgtatcaat tttaatttga ttcatcatct caattttgtt catgccatta tagtctgaca
     8761 tagttggaga aaagttttta cttgcatgag ataaaatagt aattaatgca gctgttccga
     8821 tagaaccagc aatttgttgt actgtgtttg tcatagatga accgtgtgca ttcatttctg
     8881 gctttaattg attcatcgta tgtgtcatta ctggaattaa tcctaacgcg ataccaacca
     8941 ttcgaatcgc ataaactgtt gccaacatga ttgttgatgt ttgttcatcc attacaacaa
     9001 aataagccgt agtaataaca acaatcgcca tacctactaa agcaagattt ctaggaccaa
     9061 acttttcata taaagcaccc gaagtcatcg acataaatgc cataacaatt gctcctggta
     9121 ataaagttaa cccagaatct aaagctgaac gatgcaataa attttgcaca aagataggta
     9181 aaatcgtttc cgaaccaatc atcgatacca ttgtgacacc cataataatg ataccaactg
     9241 taaattgttt atttttaaat actgcaaaat ttaataaagg tgttgatagc cgatattgac
     9301 gtaatacaaa taatgcgact aaaacaatac caccaatcat agatactaat acgataggat
     9361 tatcaaatcc tttttctgaa attgaactcg ttccatacaa taacccgccg aaacctagta
     9421 cagaaaaaat gacagaaata aaatcaatag gtactttaat tgtagttcca acatttttta
     9481 tcgttttaaa tccaattaaa aatgtcacag ctgcaattgg agcgacaaca tggaaaagcg
     9541 atctccagtt aaaatattca acaagataac ctgaaagtgt aggtccaata gctggtgcta
     9601 aaccaattac caaaccaaat atccccatat atttaccgcg ttcatgcggt tggaaaacat
     9661 ccaaaatcgt tgtcatcatc aatggcatca taatacctgc acctaacgcc tgaataattc
     9721 tagcaactaa taatgttgca aagtgcggac tcaaaccgcc gagtaatgta cctaagaaaa
     9781 agatagaaat acccactaaa aacacttgtc ttgttgtata acgttgtata acaagtgcgg
     9841 acaaaggaat aacaatccca ttagtcaata aaaaagccgt tgtcagccat tgaacttgtg
     9901 tatatgaaat ttcaaaatct ttcataatac ttggtaaagc cgtcactaat agtgtttcat
     9961 ttaataatcc aaaaaatcca cccatcagca tagtaataat catcgttaat ttttgtttct
    10021 tcgacattta aagcttcccc ctcaccatct aaaaaacgac acacattttt agatatccgg
    10081 taattatacc cattttcaaa aaaatagcac gactttatta caatttcttt aaaacgtaat
    10141 cattactatt tacatatgag tagttatcgg tcataatata tttacaagga gggatagaga
    10201 tgaaattaga tttacagact gctcgtcgta atttaaatag ccctaacatt aaaacaagaa
    10261 agcgtgcttt aaagattatt aagcaacaca aaagagcgaa ataattgttt taatttgact
    10321 atattgtatg tctaactata ttttcgagta tccctagctc gttaatataa actctatact
    10381 taactatttc cttacattac ttcacatatt taagagctgc atatacactc actccttgtg
    10441 tacatgcagc tcttttttat aagtagtatt tatctataac ttctaaatca tccgttaatt
    10501 cataaacaag cggtgcacct gttttaattt cataattaat gatatcttca tctgacacat
    10561 cttcaagata tttaatcaat gcgcgaattg aatttccgtg tgcagaaact aataccgttt
    10621 gaccatctag caaatattgt gaaatatgat ctgtccaaaa tggtatcact cgaactaaag
    10681 tatctttcag actttcggaa taaggcatca tacgtttatc taaatgatta tatcgacgat
    10741 cagctaagta agcttcacgt tgttcttcgg tttcagcagg tggtttcaca tcataagaac
    10801 gacgccaaat atgtacttgt tcctctccaa attcttttct agcatcatct ttatttaagc
    10861 cttgcaatcc accatagtga cgctcattta aacgccagct tttatataca ggaatccatt
    10921 gttgtttaga ttcagttaaa atataatgcg ttgtatctaa agcacgtgtt aataacgatg
    10981 taaaagctac atcgatgtca atgttatttt ctcttacttt ttcacctgct ctagtcgctt
    11041 cattaatacc ttgttcagat aaattaacat cttcccatcc agtaaataag tttttagcat
    11101 tccactcgct ttgtccatga cgacataaaa ttaattttgg cataacttat aacctctttc
    11161 ctttaatctt taggacgtta tttcagggat attttaagat gatttaatct tagcaatttt
    11221 cttttcaata tgttttaaaa ttcaatgatt aaatcaatta tcactagaca ttaaatacat
    11281 aaatcctatt ccccattttc attttttaat tcataaatga atcaataacc acctaataac
    11341 aaatcatatt atacaccttt gtccgtcatt tttctaaggt ttaaaaaata tttttaggtg
    11401 cgcctaaaaa taaatgtaat aaaaacgcct cctcagatat ttatatatct atgaagacgt
    11461 ttaaatacat tatagatggt ctggttctgg gtgaacgtat actgaggaaa tacctttttt
    11521 gtgcaaatga tgttcgacat tatcacaaat ttgatgcgct tctactaagg aaaggttagc
    11581 atctacaaca attgtgacat caataaacac actacttcca tggtaacgcc ctttaatact
    11641 tttaacttct tgtacttcat caacttctaa aatatcattg cgatacgctt ctaattcagt
    11701 ttcattgaaa ccatcactca acataaaaat tgcttcttta aaaataccaa aaccagtata
    11761 aacgattagt aagcctagca atgttgctaa aataatatcg acaattggga aaccgatttg
    11821 cgtaaaaatt aatcctatcg ccgttccaat gctgactaaa ctatctgata aattatcttt
    11881 ggcagcagaa tttaaagaac tacttttcgt tcttttcgct agtctttgat tgactgcaaa
    11941 tacaatcaac attacaagac cactgattaa gctgacgata attgttattg cgttaggtac
    12001 aacgtcatct tctttgaaca aacgaggtgc attttgaata actacttgga tacctacaaa
    12061 cataatgaca aatgacacca ataatgaaga aatattttca gacttcaaat ggccgtaagg
    12121 atgatttcga tcggcaggtt taattgaaat tttcaatcca ataataacag ctaaagaaac
    12181 gataatatct gtcatattgt ttaatgcgtc agctcttaca gctgcagagt taaagacaaa
    12241 acccgtgaca tacttaacaa tagataagat tatatataca atcaaactca aataagcacc
    12301 gcgttgcgcc aatttaagat tttcattatg agacatgcgt tgaaccacct tgaattagta
    12361 tagtaacaat attatgaatg atttatttta aatttacaac gtttttaatt tttattaaat
    12421 ttttataaaa ttaaactaat ttattcattc gcaaccccta aaaatatttt ttagcctttc
    12481 tgcgaatttt atgagctaga aaggcttatg cattagtagc gaagctatct aatgcgttcc
    12541 tttattttct gcggatttta tgagctagaa aggcttatgc agttgacttt ttacgtccaa
    12601 ctgcgttcct ccgtcttctt caaatttatt tgttagaaag gcgcccaact ctccctgttt
    12661 gttaactttc gtctcgaaag tttctatgtt gggaccctat gcatgagttg cgaagctatc
    12721 taatgtcttg acctaattat atagaagaaa aagtgcatca gtgacaaatc aaatgagatt
    12781 tctactctac caaactctct tcgaaagaca attttctcct ctatttatta gcaaactatt
    12841 gcatttctcc atatagtact ttcttactta aaatacgctg aatgttctga attaaacgct
    12901 tttttacata gttaacacta gttaatctat tagttaacat tagttaataa ttagttaatt
    12961 tccatttgta ttctcatgtg ataaattcta aaagcataca ataaatttaa tatgtaaaaa
    13021 gaaagggaat acacatgaaa aataaatata tctcgaagtt gctagttggg gcagcaacaa
    13081 ttactttagc tacaatgatt tcaaatgggg aagcaaaagc gagtgaaaac acgcaacaaa
    13141 cttcaactaa gcaccaaaca actcaaaaca actacgtaac agatcaacaa aaagcttttt
    13201 atcaagtatt acatctaaaa ggtatcacag aagaacaacg taaccaatac atcaaaacat
    13261 tacgcgaaca cccagaacgt gcacaagaag tattctctga atcacttaaa gacagcaaga
    13321 acccagaccg acgtgttgca caacaaaacg ctttttacaa tgttcttaaa aatgataact
    13381 taactgaaca agaaaaaaat aattacattg cacaaattaa agaaaaccct gatagaagcc
    13441 aacaagtttg ggtagaatca gtacaatctt ctaaagctaa agaacgtcaa aatattgaaa
    13501 atgcggataa agcaattaaa gatttccaag ataacaaagc accacacgat aaatcagcag
    13561 catatgaagc taactcaaaa ttacctaaag atttacgtga taaaaataac cgctttgtag
    13621 aaaaagtttc aattgaaaaa gcaatcgttc gtcatgatga gcgtgtgaaa tcagcaaatg
    13681 atgcaatctc aaaattaaat gaaaaagatt caattgaaaa cagacgttta gcacaacgtg
    13741 aagttaataa agcacctatg gatgtacaaa agcatttaca gaaacaatta gacgcattag
    13801 tagctcaaaa agatgctgaa aagaaagtgg cgccaaaagt tgaggctcct caaattcaat
    13861 caccacaaat tgaaaaacct aaagcagaat caccaaaagt tgaagtccct caaattcaat
    13921 caccaaaagt tgaggttcct caatctaaat tattaggtta ctaccaatca ttaaaagatt
    13981 catttaacta tggttacaag tatttaacag atacttataa aagctataaa gaaaaatatg
    14041 atacagcaaa gtactactat aataaatatt accaatataa aggtttgatt gataaaacag
    14101 ttttaacaac tatcggtagt ggctatggtt catacattaa acctcttgaa gtaagcaaag
    14161 aaagcgggaa cttagctaaa tcatatgcac aagtaagaaa ctatgtaact gagtcaatca
    14221 acactggtaa agtgttatac gcattctacc aaaaaccaga attagtaaaa acagctatta
    14281 aagctcaaga aacagcaaca actttcaaaa acgctttaac aggcatattc aaatcattct
    14341 ggaaataatc aatcaaaaat atcttctcta gttttacatc attatttaaa taattttcgt
    14401 aaaaaaccgt gattaaaaag aaccgttgat tctcaatcga atctgcggtt cttttttcat
    14461 tttccatcaa ttaaatgttt cttcgctatt tgtcagctca cttttttacc tgcaacttgt
    14521 taaataatcc ttacatcgtt aacgaatagt tcatcattta gttgaatcag ctcaacttta
    14581 ttaacttcaa attttcacaa actattgcac aatccattcc tctaccactt caagcacctt
    14641 aattaaacat taattcaata gaataagact tgcaaaacat agttatgtag ctatataaac
    14701 gcctgcgacc aataaatctt ttaaacataa cataatgcta aaacatcatt taacaatgct
    14761 aaaaatgtct cttcaataca tgttgatagt aattaacttt taacgaatag ttaattcgaa
    14821 aacgcttaca aatagattat tatatatatg aacttaaaat taaatagaaa gaaagtgatt
    14881 tctatgatta aaaataaaat attaacagca actttagcag ttggtttaat cgccccttta
    14941 gccaatccat ttatagaaat ttctaaagca gaaaataaga tagaagatat cggccaaggt
    15001 gcagaaatca tcaaaagaac acaagacatt actagcaaac gattagctat aactcaaaac
    15061 attcaatttg attttgtaaa agataaaaaa tataacaaag atgccctagt tgttaagatg
    15121 caaggcttca tcagctctag aacaacatat tcagacttaa aaaaatatcc atatattaaa
    15181 agaatgatat ggccatttca atataatatc agtttgaaaa cgaaagactc taatgttgat
    15241 ttaatcaatt atcttcctaa aaataaaatt gattcagcag atgttagtca gaaattaggc
    15301 tataatatcg gcggaaactt ccaatcagcg ccatcaatcg gaggcagtgg ctcattcaac
    15361 tactctaaaa caattagtta taatcaaaaa aactatgtta ctgaagtaga aagtcagaac
    15421 tctaaaggtg ttaaatgggg agtgaaagca aattcattcg ttacaccgaa tggtcaagta
    15481 tctgcatatg atcaatactt atttgcacaa gacccaactg gtccagcagc aagagactat
    15541 ttcgtcccag ataatcaact acctccttta attcaaagtg gctttaatcc atcatttatt
    15601 acaacattgt cacacgaaaa aggtaaaggt gataaaagcg agtttgaaat cacttacggc
    15661 agaaacatgg atgctacata tgcatacgtg acaagacatc gtttagccgt tgatagaaaa
    15721 catgatgctt ttaaaaaccg aaacgttaca gttaaatatg aagtgaactg gaaaacacat
    15781 gaagtaaaaa ttaaaagcat cacacctaag taaacagttc aatcatttta taaaatcctg
    15841 ggacacttca tacttgtctc aggatttttt aacatattga atcagcctca taacattaaa
    15901 ttattttatc gtacaactga tttttataag agcaaagtat cgcatcatag tagatacaca
    15961 aataataaaa atgaaacaat ttaacttgaa agcttaaata aatattctca agttaataaa
    16021 taattaactt ttagatggat tcatcaaaaa tcgcaaaaaa gaacaatttg tattttacaa
    16081 acattaatta aaaataaaag caagacattc gtgcaatcgg ttgccttaaa atgtttacaa
    16141 ctgtcaacaa taccaaggtt ttattaacta tatttctcac aaaattagct tttagcattc
    16201 caaacaaaaa aggttaaatt gaacggaatt atggcatttt taacttaatt gtaaaaaaag
    16261 ttgataatgg tcaattgtta atgaacagtt aattataata acgtccaaaa tatattatta
    16321 tttaattaag ttaaataaaa ttatagaaag aaagtgaaaa ttatgcttaa aaataaaata
    16381 ttagctacaa ctttatctgt aagcttactt gcccctcttg ccaatccgtt attagaaaat
    16441 gctaaagctg ccaatgatac tgaagacatc ggtaaaggaa acgatgtaga aattatcaaa
    16501 aggacagaag ataaaacaag taataaatgg ggcgtgactc aaaatatcca attcgacttt
    16561 gtgaaggata aaaaatataa caaagatgct ttgatcttaa agatgcaagg attcattagc
    16621 tctcgaacaa catattataa ctataaaaat acaaatcata ttaaatctat gcgttggcca
    16681 ttccaatata atattggttt gaaaacaaat gataaatatg tttctttaat caattattta
    16741 ccgaaaaata aaattgaatc tacaaacgta agtcagacat taggatacaa tattggtggt
    16801 aatttccaat cagccccatc acttggtggt aatggatcat ttaactattc taaatcgatt
    16861 agctatacac aacaaaatta tgtaagtgaa gtagaacaac aaaactcaaa aagtgtttta
    16921 tggggcgtca aagcgaattc attcgccact gaatcaggtc aaaaatcagc atttgatagc
    16981 gatttatttg taggctacaa acctcatagt aaagatccta gagattattt cgttccagac
    17041 agcgagttac cacctcttgt acaaagtgga tttaaccctt catttatcgc aacagtatct
    17101 cacgaaaaag gttcaagcga cacgagcgaa tttgaaatca cttatggaag aaatatggat
    17161 gtcactcatg ccattaaaag atcaacacat tatggcaaca gttatttaga tggtcacaga
    17221 gtccataatg catttaaaaa tagaaactac actgtgaaat atgaagtcaa ttggaagact
    17281 cacgaaatca aagtgaaagg acagaattga tatgaacatg aataaattag tcaaatcatc
    17341 cgttgctacg tctatggcat tattattact ttctaatact gcaaatgctg aaggcaaaat
    17401 cacaccagtt agcgtcaaaa aagtagatga taaagttact ttatacaaaa ctacagctac
    17461 agcagattca gataaattca aaatttcaca gattttaaca ttcaatttca tcaaagataa
    17521 aagttatgat aaagatactt tagtgcttaa agctgcaggt aatattaact caggttatga
    17581 aagaccaaat cctaaagact acgacttttc aaaaatatat tggggtgcaa aatacaatgt
    17641 gtctatcagt tcacaatcta atgattcggt taatgtagta gactatgcac ctaaaaatca
    17701 aaatgaagaa tttcaagttc aaaacacttt aggctataca tttggtggtg acatcagtat
    17761 ctctaatggt ttatctggcg gacttaacgg aaacactgct ttttctgaaa caattaatta
    17821 taaacaagaa agttacagaa caacattaag tcgcaacaca aattataaaa atgtcggttg
    17881 gggtgtagaa gcacataaaa ttatgaataa tggttgggga ccttatggac gagatagctt
    17941 ccacccaaca tatggtaatg aactcttctt agctggcaga caaagcagtg catacgctgg
    18001 ccaaaacttc atagcgcaac accaaatgcc attattatct agaagtaact tcaatccaga
    18061 atttttaagc gtactatcac acagacaaga tggcgctaaa aaatctaaaa ttacagtaac
    18121 ttatcaacgt gaaatggatt tataccaaat tcgttggaat ggcttctact gggcaggcgc
    18181 gaattataaa aactttaaaa ctagaacatt taaatcaaca tatgaaattg attgggaaaa
    18241 tcacaaagtg aaattgttag atacaaaaga aactgaaaac aataaataat tagtaaaaca
    18301 cggtcgccaa cagtaattgt gacgaccgtg ttttgattta ttatcttagt aagactgcca
    18361 ttctttttct caatatgaga tataaaggaa tagctacaat taaagtgaat attacgcctg
    18421 gaatcgcgtt taataacacg acccacacag gtaaatttaa aatgaccgat agtatcaatc
    18481 ttgataccaa actgcctaat acacttgcta aaactaatga tagtacattt attttcaata
    18541 aataaacaac tgcaatagct ataactctaa atataataga aataatcaca ttaatcggat
    18601 taaatacgcc aaatactagt aataataggc tagataataa tccacctaaa aagtactttt
    18661 taattccaaa gaaagctaat atcaataatg ctgctggtgc agataattga aaatctaatc
    18721 ctggtataat ggatggtatt ttcaaaactg ccaaaatggt taaaatcgca gcaatgacac
    18781 taatttgagt aatatctttt gatgtcatac taaaacccct ataccgtttc ataaacaact
    18841 tgcttcggtg tgctttctaa aaataatatg taatgattta aatcaatacg atcgtccaca
    18901 aatattattc tgcctccata tctcgtatta actggtttaa tatcaaataa tcgatggtaa
    18961 ccaattttag cagaggcaaa ataacctgtc gtatacgtta agtcatcgga cacgcaaagt
    19021 tctcctttga catacggatg cgcattgata caactagcaa ttgctaaggc atcagtcact
    19081 ctttcattaa gatcaccttt attatttata tcttcaaacg aaaaatgtgt tgccctaatc
    19141 cccctatgtc caaatgaatc taaacgttta ccagatatag cagatagaat aatagctcct
    19201 gtataaaccg tttcattttt aatatatgtc atcccttgat ttagcgcttg ttcagtgaca
    19261 ccacattttt gtgttaaatg ttggagattc gctttatcat cctcaataat ttgtaatgct
    19321 tttatttgtt taatcggttc cataattttt tgtattttta tattgagaaa atccggttgc
    19381 ccattttcat gaaaaaaacc tttattataa aacctttgta ccgtctgttc tattttgtca
    19441 aactcacaaa tcgtttcagc accactaata tgaatatctt gattgctaga acgcattttt
    19501 atactataca tgacgatcac ctcaatcttc ttgatgcaaa atttcaaaca acctatctat
    19561 atcttgttca gtatgaaaat acgataatga tattcttaac attggcttag tcacagttgg
    19621 ataccttaaa taacttgtaa acacatgatg ctttaataat gtttgatgaa tgttctcagc
    19681 cgcttctatg tcatcaaact caataaactt aatcggcgag tttgaactat tataattaac
    19741 attgagtgct tttaactttt ggttaaaata tttactcaag ctatttaatt tagtgcgtct
    19801 atcatcagca tttattaact tttcaatatt tctttttata aaatacaaat tataaattgg
    19861 caaactactt gagtagatga gtggtctacc gtgattaatt aacatatcct tcacatcatt
    19921 tgaacttaaa atcacacccc cgtatgcacc acatgcttta gataaactag aagtgagtat
    19981 atctatgcct tgataattcg agtaattctc tattccaaaa ctatgtgaaa catcgagtat
    20041 cagtgttgcg ttatatttat gctttaatga gactaattga ccaatatcca caacgtcgcc
    20101 attcgttgaa aatacactat cagatatgat tattttttgt atattttgat tagggtattc
    20161 cgctaacctt ttttctaaat cagcaatatc taaatgctta tatatcactt tttctaaacc
    20221 acttagctta ataccgtcaa taatactcgc atgattttct tgatctgaaa atacgacaca
    20281 atttgtattt ttgaaaatat taaataacgc caaattagca tcataaccac tatttaagat
    20341 agtacatgca ctatatccga gccagcctgc taacattgat tcaatctctt cataaactgt
    20401 cgaacttcca ctaatcaatc ttgaacttga taagtgataa ctatacttcc gcataaaact
    20461 ttcgaaatca tccttatcaa acgctatttg acctaatcct aaataatcat tagatgtata
    20521 gttcgtacat cgcttatttt ctacttcaat atactgtcta tctatatacc ctaccgattt
    20581 aagcgaccga tataaccctt tctgttgtaa taaatcaatt tgctcttgaa acatcattct
    20641 tgttttcctt attttcacaa gtgtcataat caatttcaaa gcccaaatca ttaatcatat
    20701 cgtagtctaa ttggttcggt tgcccaccag taattagata atcaccgaca aatattgaat
    20761 tcgccgcttt taatgctaat ggctgtaacg aacgtaagtt gacctctctt cctccagcaa
    20821 tacgaatttc tttcgtagga ttgattaatc ggaataatgc tacgattctt aaacatttca
    20881 ttggtgttaa atcatccatg cttccaaact ttgtaccttt gattggatgc aaaaaattaa
    20941 tcggaatact gtcggcatcc atttctttca aagcaaatgc catatcaaca atatcttgat
    21001 tagattctcc cataccacaa atcacgccag aacatggtga tatattattc gctttcatta
    21061 gttctatcgt atctgttcta tctttataac tatgcgttgt cacgacgtta tcatggtaat
    21121 tttcacttgt attaatatta tggttatatc tgtctacacc agctgactta agtttcttag
    21181 cttgttcgtc attcgttaat cctaaacatg cacatatttt cagttgcggg tgttgagatt
    21241 taatcgttct tacagtgtta ctaatatgat caacttcttt atcgctcggt cctctaccac
    21301 tcataacaat acaatatgtt ccaatatgat tatcatgtgc cacctttgct ccatcaataa
    21361 tttgttcctc tggaattaga gcatatcgct gtttttgttt aatttctcgt gattgtccac
    21421 aatacccaca attctcagga catataccac ttttagcatt taaaatcatg tttaatttta
    21481 cttttttacc gaaataatgt tttcttaaaa tatacgcctc atttaataaa tctaaggtat
    21541 caatattagt atcctcataa attttcaata cagtctcttt tgttaattgt tccccttgta
    21601 atatgcgttt agccaaattc atattaacac ttcctatcta aaatgttatg tactgccttt
    21661 ttaaaaatac gtaccatcgt ttttacttct ttatttgaca tacttaacac tggcacaaac
    21721 gtaatgacat tttctaaatt tctaatcatt agcccgtttt ctttacaatt acgtacaata
    21781 cgttctactt tttcaatttc taacggcgtt ttactatctt tatctgtaac taattcaact
    21841 ccaaacatta agcctcgtcc tcttacatca ccaacatttc gatgacgctt cagcgcatgt
    21901 aactgtttct caagtgttga tgatgtcgtt tcaatttgtg acaataactt acatttttca
    21961 taaagtctta tattttctaa tgcaaccgta caaacgattt gatttccggt gtatgtatga
    22021 ccatggaaaa aggtattcac accatgcgaa tcacttaaaa atgcattgta tattttttga
    22081 gatgtcaatg tagctgcaag tggtaagtag ccacctgtaa tcgccttacc taaacacata
    22141 atatccggtt gaacatcttc atgattgcat gcaaacatct ttccagttct cccaaaacca
    22201 actgctacct catcacaaat taataagaca tcgtattttt tgcacaattt ctcgacttct
    22261 ttcaaaaagc ctttaggatg aacaaacaag cctgtcgcac cttgaatcaa cggttccaat
    22321 ataaaccctg cgatttcatc atttctttca attagaattt gctctatatt cgttaaaata
    22381 gctgtcatca tttcattttc agtatcgtaa ttactgcgat agaaagatgg gctttctact
    22441 tgaatatttt caaatattaa gtctttaaat attttatgaa aggtcttgat accaccaaca
    22501 cttaccgcac caatcgtatc cccatgataa ccgtgattta gcgttataaa cttgtttttc
    22561 ttggcatatt tttctctatc aatatttttc caatactgat atgccatctt tattgcgatt
    22621 tcaacagacg cactgcctgt atcagaataa aatacttttc ttagattact tggcgtgatt
    22681 tcgattaatt tttccgcaag ttctattgac ggaatatttg atgatcctag cagcgtagaa
    22741 tgggcaattt tattgagttg ctttttaatt accttattca agtatttgtt attatgacca
    22801 tgcacattga cccacaacga tgcataacca tctaaatatt tattgccatt cgtatcgtaa
    22861 aggtaactac cctttccttt ttcaatgatg attgcttctt ctttgctata tacacccatt
    22921 tgtgtaaatg gatgccaaac atattctgag tctttttgtt taagttgttg tgtgtaattc
    22981 atttttaact cctattaatt gttttaaaaa tgcttctgag aaattttcat acgtggcatg
    23041 ttcttcaaat gtatagactg ttttatttgt taattttcca atcgtaattt gattgtcttt
    23101 ttcaatatag ctgcctgtat agcgattcat tattaaaaaa ttactcgccg atacattctg
    23161 attaacataa tcttggtgaa caatggcatc gctaatagca cctaattttg atggcaacac
    23221 actgatgaca caatctgcac aatcattgat tagatcttta gtcatgtaga aatcatctgt
    23281 accttcatat attggtacgg caattccccc agcaccctca attaagataa aatcaaattc
    23341 cttatctaaa acttttacct tatctaatac acactgctta tttagaaaaa tttgatttgc
    23401 cattttaaat gcaaggtgtg gtgatacagg ttgcttaaaa gtataaagtg acgttatgtc
    23461 atagcttaaa tcacattcat ttttaaatac ttctaaatct ggaaatgtcc cgtcttggcg
    23521 ttcctcagtt tgaaatggtt taaaaataca aactcgatga ccacgtgttt tcaaagcatg
    23581 gtataaatgc tttgtaacat aggttttgcc tacatcagta ttcgtacttg taataaaaat
    23641 cctcataaaa aataagcacc tcaatccgaa tgtaaacttt tataattaat aagtttacat
    23701 ttaaggcgcg tgtttttcaa gaaaacatga atttaaactt ctcagtataa ctataaattt
    23761 tagcaaaatt ttgaccttgc tattgaaatg actaaggctt catacggaat tcgggcagat
    23821 ataaacagcg attcgataaa atatgtcagt taaaattaat aattgcatta tttcttaata
    23881 aaaagacatc ctagctttac catgttgtga aacaatccat taattgtaat attacgttga
    23941 gcgccttgtt catcaatgct ataattagtt acataataaa ttgaacatct aaatacacca
    24001 aatcccctca ctattgccct agtgagggga tttattttgc tgttgattat ctatcaccct
    24061 ttttatttcc aaattctgat tccatattaa actattattt ataatttcca gcctgctgat
    24121 ttacttttta ttcttataaa gtcttgatac attcctggtt ttcgaattaa ttcatcatga
    24181 ctaccttttt gaattatttc accttcattg agcactataa tctgatctgc atttttaata
    24241 gtttcaagtt tatgtgcaat tgtaattact gttttgcctt tacttaattc attaattgcc
    24301 gtttgaatca gctgttcatt ttcagggtca atacttgcag ttgcttcatc taaaataatt
    24361 attggtgcat cttttaatat cgccctagca atagaaatcc tttgcttttc tccgccagat
    24421 aaattactac ctttttcatt tagcattgtt tgatatccct caggtaatga catgatgaag
    24481 tcgtggcaac atgcttgctt cgctgcacga ataatttctt ctttcgttgc acctggatcg
    24541 ccaaacaata tgttattttc aatcgtatca ttaaataaat acaccttttg aaatactgca
    24601 ctaattttcg acattaacgt acttaatgtc atatctttaa tatcaacacc atcgatgcga
    24661 atatttccat catcgatatc ataaaagcgc aagagtaagt gacataatgt agatttccca
    24721 cttcctgaag gtccaattat tgctgttgat gtttgtgtag gtatctcaaa attcacattc
    24781 tttatcactt gtttatcatc atatgaaaaa ttcacgtttt gaaaagcgat attttcattt
    24841 ttaatcgtca aattttttcc attctcatcc agttctggag cattttttat cttttcaata
    24901 tcatctatcg tcatatctat catttcaagt acgtgtgcag cactattaat attttcaaca
    24961 ctatcaaata tcacaaatga aaaaattgaa atcataataa gggtaggtaa atcaatacta
    25021 tgattcatat ataataagca tgcaactaat acaatcatta ttgaaacaac ttttaaacta
    25081 agtaaatgca ataaattgaa tgggataaat tgcatttcta tttttgtatt tatacgttta
    25141 cttccattga ctgcttggtt aaaactttta agactcgtat tttctttcgc gaatgatttt
    25201 attacttgaa taccacgaat aacctccaat accttttcca ctaattggtt ttgtacatta
    25261 tgatacgctg gcgcattttg tcgactcttt ctttctaata attgaatcgc aaaaaatgat
    25321 agtaatacgc caatgcatgc taataatgat acttgccacg aaactacaag tagagacaat
    25381 atgagtactg taattaatat gtatccatta acaacaacgt ccaccatttt catagcaaag
    25441 ttttctaaaa aggttaaatc tgttgttact attgttgtta actcatttga atgatgcgaa
    25501 ttaaaataac ctaaccttac atttttcaat ttatccccta tatctaaacg ttctttcgca
    25561 ctcatttcat aagcgatgct ctcatggctt ttgcttttga aatatgctgt aataaatcgt
    25621 ccaatcacta ataaaaccat gataattaca acattcaata tatctttcat ataaataggt
    25681 ttatgagata gcacattatt aaatattttt gcaactaaaa agataggtaa cgcaataaaa
    25741 atagcattta aaaatgacat gctaaatcct aaaatcattc ttgctttata tggtcttatc
    25801 cagtttaaaa ttttaaatgt aatttgaaac atatttgacc tccttttctc attagtttaa
    25861 ttatgtccag tattgattcc ccaatctttt gtatgcatgt gcgtatccca cattttctta
    25921 taattaccgt ttaattttag caataagtga tgtgaccctt tttctaaaat ttgttgttta
    25981 cctaaaacaa taatttgatc tgcatgttga atcgtagata accgatgtgc aataacaatc
    26041 aatgttttat cctgcgtcaa cacatttaat gcttcttgaa ttttttgttc gttgtctgga
    26101 tcaacgtaag cagtcgcttc atctaataca atgataggcg catcttttaa tatcattctt
    26161 gcaatagtga ctcgttgttt ttcaccacca gacaatttat ctcccactgt accaacattc
    26221 gtatcatatc catctggcaa cttttcaata aattcatgac attgtgctaa cttggcagct
    26281 ttttcaactg cctcatccgt agcttctgga ttgccaagtt taatattttc tttaaaagta
    26341 agatttaata aaaagttatc ttgtccaaca aaaccaacta aatcgttaag ttgtttcgat
    26401 tcaatatctt ttatatctat accgccaatc gtaatttcac ctgaagtcac atcccagtat
    26461 cgtgatataa gcttggcaat ggttgactta ccgctaccag atgccccgac gatagctatg
    26521 aaattatttt ctggtactgt aaatgataaa tgcttaaaga ccagatcgtc cttatctttg
    26581 ttatatgaaa atccaacatt attaaaagca atttcataat gttgaggctt cttaaatttt
    26641 gttgacaata ctaactcttc tagacttaat atttgattca cttcagttaa tgcgtattgt
    26701 atagacttta aatgatttac ataattagta aaattcttaa tcggtgctac tacacctaaa
    26761 gataatacga tgcataggaa aaattccgca tagtttaatt ggttaataga tatcaaatac
    26821 atgccgaccg gtaaaatccc taaaaatgtt gaaggtaata cactagctcc taaattcata
    26881 tacccccatg tatttttaaa ccaattcaaa gtgtgaatct tataattatc tactgcatct
    26941 ttatattttt tatatgaact ttgagattga ttaaatgttt taatcacttc aatgccttcg
    27001 ataaactcta caatcgcgct attcatataa ttattcgatt tcatttgttc agcatatgtc
    27061 tcattaaatc cagacataac ttttttaaaa gcgaaaattg aaattggtat cgttactaat
    27121 aaggcactag ccatacgcca atcaatgagc attatgtata aaaagatagc agctgacaaa
    27181 agtaagtttc ctataacttc aggaatcata tgtgctaaag gtaattctat tgtttcaacc
    27241 ttatcgacaa atatattttt taattcacct attttcttag attccactac gcctaaaggg
    27301 agacgcatta atttttgagc taatttttta cgaatttcag ataaaatttc atatgccgta
    27361 atatgtgata gcatcgttga cgctccaaaa caacacactt gtgaaatata agcgattaaa
    27421 acaataaaga tataaaccat aatcgaatta atcgtatatg cattgttaat catcattaaa
    27481 ataattttaa agactgccca atatggaact aatccagaaa agacactgat gatagacaac
    27541 aaaattgata acataatttt ccatttacta tcagaagcaa atccgctgag tatctttatc
    27601 caatttgtct gattgttcat cgtatatgta actcctttca attaataatc taaattaagc
    27661 cg
//
LOCUS       DAKSQE010000024        27034 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.024, whole genome shotgun sequence.
ACCESSION   DAKSQE010000024 DAKSQE010000000
VERSION     DAKSQE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 27034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27034
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.024"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..1070)
                     /locus_tag="O0V33_000887"
     CDS             complement(<1..1070)
                     /locus_tag="O0V33_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCX8975550.1"
                     /translation="MTMIYRNNFIVFVLSFFISILLYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKDNWLSIIVVSISILLIALVMA
                     FIFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYIIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRE"
     gene            complement(1104..1217)
                     /locus_tag="O0V33_000888"
     CDS             complement(1104..1217)
                     /locus_tag="O0V33_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975551.1"
                     /translation="MYIRHGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
     gene            complement(1265..2551)
                     /gene="hemL"
                     /locus_tag="O0V33_000889"
     CDS             complement(1265..2551)
                     /gene="hemL"
                     /locus_tag="O0V33_000889"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCX8975552.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGDDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRDITTEYGALLIFDEVMTGFRVGYHCAQGHFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            complement(2599..3573)
                     /gene="hemB"
                     /locus_tag="O0V33_000890"
     CDS             complement(2599..3573)
                     /gene="hemB"
                     /locus_tag="O0V33_000890"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCX8975553.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            complement(3576..4244)
                     /locus_tag="O0V33_000891"
     CDS             complement(3576..4244)
                     /locus_tag="O0V33_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCX8975554.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLESF
                     NQTNQKILLPSSELARPLLSAALSEDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            complement(4266..5192)
                     /gene="hemC"
                     /locus_tag="O0V33_000892"
     CDS             complement(4266..5192)
                     /gene="hemC"
                     /locus_tag="O0V33_000892"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCX8975555.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQKEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKKVSNKLKEQGAYEIIKRLNEQH"
     gene            complement(5234..6049)
                     /gene="ccsA"
                     /locus_tag="O0V33_000893"
     CDS             complement(5234..6049)
                     /gene="ccsA"
                     /locus_tag="O0V33_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HCX8975556.1"
                     /translation="MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLG
                     IVWVLQTISLSIFIIQTKHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNL
                     IGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQY
                     RNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFID
                     PKVILSSIITVLYGSYILLRINKWLNSRYLIYYNIILFCLNMINLFFATHFVN"
     gene            complement(6071..7417)
                     /locus_tag="O0V33_000894"
     CDS             complement(6071..7417)
                     /locus_tag="O0V33_000894"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCX8975557.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WVSMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAKCPHEQAKQQKESKVKEISARRIFSFE"
     gene            complement(7634..8224)
                     /locus_tag="O0V33_000895"
     CDS             complement(7634..8224)
                     /locus_tag="O0V33_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCX8975558.1"
                     /translation="MKVNPNNIELIISAVKEDQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIQNNKQQQIWNLIEPYIS"
     gene            complement(8378..9640)
                     /gene="clpX"
                     /locus_tag="O0V33_000896"
     CDS             complement(8378..9640)
                     /gene="clpX"
                     /locus_tag="O0V33_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCX8975559.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAITELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDDVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
     gene            complement(9791..11092)
                     /locus_tag="O0V33_000897"
     CDS             complement(9791..11092)
                     /locus_tag="O0V33_000897"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCX8975560.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDESQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            complement(11140..11250)
                     /locus_tag="O0V33_000898"
     CDS             complement(11140..11250)
                     /locus_tag="O0V33_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975561.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            complement(11255..12184)
                     /locus_tag="O0V33_000899"
     CDS             complement(11255..12184)
                     /locus_tag="O0V33_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975562.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLEQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLTDFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNMYSYKLISLMIEYLRFANCTQELTIEKYGMDITFVPANLK
                     GLEHTTLKEKVIPNVMETLNEGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            complement(12203..12811)
                     /locus_tag="O0V33_000900"
     CDS             complement(12203..12811)
                     /locus_tag="O0V33_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCX8975563.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNTFNGFLNKNKLEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            complement(12953..13309)
                     /gene="rplT"
                     /locus_tag="O0V33_000901"
     CDS             complement(12953..13309)
                     /gene="rplT"
                     /locus_tag="O0V33_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCX8975564.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            complement(13356..13556)
                     /gene="rpmI"
                     /locus_tag="O0V33_000902"
     CDS             complement(13356..13556)
                     /gene="rpmI"
                     /locus_tag="O0V33_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCX8975565.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            complement(13585..14112)
                     /gene="infC"
                     /locus_tag="O0V33_000903"
     CDS             complement(13585..14112)
                     /gene="infC"
                     /locus_tag="O0V33_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCX8975566.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLISQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     misc_feature    complement(14141..14266)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(14341..15834)
                     /locus_tag="O0V33_000904"
     CDS             complement(14341..15834)
                     /locus_tag="O0V33_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCX8975567.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVVTVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGGNDVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFETFGKTNKNGVPYMSLLVTAIIVVIIFVVQSVTEGAYQYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKWFPLGPIFAAILCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     gene            complement(16261..18198)
                     /gene="thrS"
                     /locus_tag="O0V33_000905"
     CDS             complement(16261..18198)
                     /gene="thrS"
                     /locus_tag="O0V33_000905"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCX8975568.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            complement(18423..18518)
                     /locus_tag="O0V33_000906"
     CDS             complement(18423..18518)
                     /locus_tag="O0V33_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975569.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPDQDRYLK"
     gene            complement(18611..19531)
                     /gene="dnaI"
                     /locus_tag="O0V33_000907"
     CDS             complement(18611..19531)
                     /gene="dnaI"
                     /locus_tag="O0V33_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCX8975570.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            complement(19531..20931)
                     /locus_tag="O0V33_000908"
     CDS             complement(19531..20931)
                     /locus_tag="O0V33_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HCX8975571.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPATSSSTAKSTEANPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTLVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEQDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSQ"
     gene            complement(20932..21402)
                     /gene="nrdR"
                     /locus_tag="O0V33_000909"
     CDS             complement(20932..21402)
                     /gene="nrdR"
                     /locus_tag="O0V33_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCX8975572.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            complement(21612..22637)
                     /gene="gap"
                     /locus_tag="O0V33_000910"
     CDS             complement(21612..22637)
                     /gene="gap"
                     /locus_tag="O0V33_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCX8975573.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDDQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPHSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            complement(22807..23430)
                     /locus_tag="O0V33_000911"
     CDS             complement(22807..23430)
                     /locus_tag="O0V33_000911"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCX8975574.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEGD"
     gene            complement(23446..24318)
                     /gene="mutM"
                     /locus_tag="O0V33_000912"
                     /pseudo
     CDS             complement(23446..24318)
                     /gene="mutM"
                     /locus_tag="O0V33_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
     gene            complement(24334..26964)
                     /gene="polA"
                     /locus_tag="O0V33_000913"
     CDS             complement(24334..26964)
                     /gene="polA"
                     /locus_tag="O0V33_000913"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCX8975575.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLADIDQSASGEDAIEKTFEIETSFDNVDFTSLKEATIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDIINYAELVSWLENPNTKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIYTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGFVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
BASE COUNT         8338 a         5203 c         3675 g         9818 t
ORIGIN      
        1 tcttcacgct tattcgaacg atattttaaa aagtaattaa ttcctggtgc aataataaat
       61 aggatgaaaa agattcggaa aatatgataa ctcgtaatca tagcaacatc ggcaccagta
      121 gctaatgcaa ctaaaactat ctgattaacc cctcctggtg ctgcaccaag aaacaattca
      181 ttaataggat tattatcaaa gaaatgtatg atataaacca tgattagcgc accaattatc
      241 aacataatat tttgaattgt aattgcgatt gctagtctac cttttaaatc tgacaataaa
      301 tgcgcaattt gaactccaat tctaatcata tatattagtt gtgccatgtt caacaaccaa
      361 tgatctagtg taaatgttag acctgtagaa aaattccaaa caattaatac aatgagtggt
      421 gctaataatt gaaatgttgg aaactttatt ttagacataa ttagataaac tataaagata
      481 gctatcgcta aaataactat ttgccctatg tttaatactt gtgataaagg caagactttt
      541 gttaacttac cattcgcatg catgttacca tcatgaaaaa aatatgaaat gaacggtact
      601 aaaacaacaa caaatataat tcgtgatgtt tgcgttaagc taacaactaa caaattagca
      661 cgtttgtctt gttcagccat gaccaacatt tgtgttagtg ctcctggtat aacacttaaa
      721 atagctgttt ctgtattaat acgtgcaatt tttttaaaaa taaatgccat tactaaggca
      781 attaataata tcgaaataga tactacaata atcgaaagcc aattgtcttt aatatccata
      841 acgacatttt tcgtaaacgt tgatccgatt tgcacaccta atagtacaat tcctaattca
      901 ctaagtaaga atggccattt aatatcaagt ttgaaaactt ttacacaaat gattgatgcg
      961 ataataggac caaacataaa tgggagtaat acgtgcgatg aatacagtag aatactaata
     1021 aaaaatgata aaacgaacac aatgaaatta tttctatata tcattgtcat gttttccact
     1081 tctttcaata aaaaataaaa tgactaaatt gctgcttgag cttcacgttt gttaagataa
     1141 caatatccgc tagcagtttt gactacaaag catatatgga ctttcactat caagtcgccg
     1201 ccatgcctga tatacattta aaaagagcct gaacaaagtt caggctctca atttgtccgt
     1261 atatttattt tacaatacga cttaaagccg tatcaaatgc ttgaatcgtt ttttcaatat
     1321 cttctttcgt gtgtgccgta gataagaatg taccttcaaa ttgagatggt ggtaaaaaca
     1381 caccttcttt tgccatttca cgatacattt ctgcaaataa tttcaaatca cttttattcg
     1441 cttgttcaaa attagttaca ggtccttcat ttaagaaata accaatcatt gaacctgctc
     1501 tatttacagt tattggtaca ttgtgtttag caaatacacg ctttaaaccg tcttcaagta
     1561 tatcgcctaa catattaaaa tactcatatg tctctggcgt taattgactt aacgtttcat
     1621 aaccacttgt cattgcaaga ggatttcctg ataacgtacc agcttgataa atatttccta
     1681 atggtgctat atgatccatg atttcttttt taccaccaaa agcacctact ggtagtcctc
     1741 caccgataac ttttcctaag caagttaaat ctggtgtcac accaaagtga ccttgtgcac
     1801 aatgataacc gactctgaaa ccagtcatta cttcatcgaa aattagcaat gcgccgtatt
     1861 cagtcgtaat atctcttaat ccctgtaaaa aaccttcaat cggcggtacg acacccatat
     1921 taccagcaac aggttctacg attacaccag caatatcgtc tccaaatttt tcgaaagcga
     1981 ttttaagtgc atctaaatca ttgtatggaa ctgtaattgt atttttagca ataccttcag
     2041 gcacaccagg agaatccggc aatcctaatg ttgccacccc tgacccagct ttgattaata
     2101 acgaatcact atgaccatga tagcaacctt caaattttac aattttattt ctgccagtat
     2161 aaccacgtgc taatcttaaa gtatccaatg tagcttctgt accagatgac accatacgca
     2221 ctttttctat tgaaggtact cggtcaataa cgagctgcgc caatttattt tcaagtaatg
     2281 ttgatgcacc aaaacttgta cctttatcaa ttgcttcatg taaatgacta ataacttgag
     2341 ggtctctatg tcctaaaata agcggccccc aacttagtac atagtcgata tactcgttac
     2401 catcgatatc ataaattttt gagcctttac cgtgatccat aaaaattgct ggtgtatcta
     2461 ctgatttaaa tgcgcgtact ggactattta caccaccagg cattaaagtt tcagcaacct
     2521 tcattgcttc ttctgacttc gtatatctca taaagtaaat tcctcctata gtttatggaa
     2581 aatcatacat ataaaacctt atttatctaa atagcgacaa atgtcctttg caaaatacgt
     2641 aataatcata tcagcacctg cacgtttcat tgaaaccatt tgttccataa cgacacgttc
     2701 ttcatcaatc caaccatttt gtgccgctgc tttagtcata ctatattctc cactcacatt
     2761 atatgcaaca actggaacat tcgtatgatt tttaacgtct cgaactatat ctaaataact
     2821 tagagcaggt ttaacaatca tcatgtcgca cccttcttta agatcacttt ctaattcacg
     2881 aagtgcttcc aaacggttag cagggtccat ctgatacgtt tttctatccc caaatgatgg
     2941 cgctgaatct gctgcatctc taaaaggtcc gaagaaactt gatgcatact tgacaccata
     3001 actcattata ggaatattgt aatagccggc ttcatctaat ccacgacgaa tttcagcaac
     3061 aaaaccatcc atcatattac ttggtgcaat aatatcagca ccagcttcta cttgagaaat
     3121 tgctgtttta acaagtagtg gcaatgattt atcattgtca acgtcatgtg tatggtcatc
     3181 aatcacacca caatgaccat gatcagtata ttcacataaa caagtgtctg caacaattaa
     3241 taagtcatca tacatttttt tagcaatacg tgttgcctgt tgaataacac catcgtgaat
     3301 gtatgcacca gtacctatat catcttttga gtttggaaca ccgaaaaaca taatggcacg
     3361 tatgcctaag tcataagctt cttttaattc actttcaagt aaattcaaac tgatttggta
     3421 tacacctggc aatgacttaa tttctttttt cacatcgtct ttttcaacta caaaaattgg
     3481 atatattaaa tcttcttttc ttacatgatt ctctctaacc atatctctca ttgtcgctga
     3541 tgatctcaat cttctatgtc tatcaaattt catttttagc ccctactttc taaaatcttt
     3601 tcaattagtg attcgagtgt ttgaatttct gcaattgtta ctggctgttg atatgattta
     3661 atggtccgtg ctgtttgttc tccaatagca aaatacgact tgaattttgg tacaaatcct
     3721 tcattaaaat aataacgtac tgccgacgaa cttgaaaatg ttaatgcatc gatttgttga
     3781 tgttctatca tttctttaac atcttgtata ttttgtttgt taggcactga agtatataaa
     3841 tctattttaa caacttcatt atcttcagat aacgctgctg ataacaatgg tctcgccaat
     3901 tcactcgaag gcaaaagtat tttttggtta gtttgattaa atgattctaa aaatccttct
     3961 tgagaaaagt cgtttggcat aaaatcaact cgaatgccaa gtgattcaca atattgcgct
     4021 gtcttacttc ctatcacagc aatgttatca acattaattc cttttagata tttataaaag
     4081 aatttcacag catttttaga tgaaaaaata agccagtcat agcgttgatt taacaaatga
     4141 atatcaaaat ttagtggctt tatatcaata aaaggtttgt gaataattga tactaaatca
     4201 ctttgcatgt catttgtttg tgtcataact acaactggct tcatatttaa acgtcactcc
     4261 attatttaat gttgttcatt taagcgtttt ataatttcat aagcaccttg ctcttttaat
     4321 ttgttactca ctttttttcc taactcaacc ggatctgttc cgttcattgt atattcaaat
     4381 cgttctttac catctggggt cataattaaa cctgtaaatt cgatttcttt ttgatctgag
     4441 attgtagcat atcctgcgat tggcacctga caactaccat ccatttctgc taaaaacgtt
     4501 cgttcagcag tcacacattt tgcaacctca tcattatgta ctttgcttaa taatgttaat
     4561 agttcttcat cgtcactacg acattctatc cctaaagcac cttgtccaat tgcaggtaac
     4621 aatgtatctc tatcaagata agatgttaca atatcatctg accagcccat tcttcttaaa
     4681 ccagctgcag ctaaaataat cgcatcataa tcttcagttt gtaacttttc taatcgtgta
     4741 tctatattac ctctaatcca tttaatctct aaattaggat acttagataa tatttgtgca
     4801 ccacgacgta atgaactagt accaataata ctaccttctg gcaattggga tagtggtgta
     4861 tgtgttttag aaatatacgc atcaaaaggt aattctctat cagggataca acctaatgtt
     4921 aaaccttccg gaattacact tggtacgtct ttcagcgagt gtattgccat atcgatattt
     4981 ttttcaaaaa gttcatgttg tatttcttta acaaataagc ctttgcctcc gactttagac
     5041 aattgtttat ctactatacg atcgcctttc gtgacaattt ctttaatttc aatttctaga
     5101 tttggctcga cagcttttaa tttatcaata aattgctggc tttgtgttaa agctaattta
     5161 cttcttctgg agccaacgac taatttacgc atgttcaatt cctcctagga acggattgct
     5221 ctagattatt ttcttaattc acaaaatgtg ttgcaaaaaa taaattaatc atatttaagc
     5281 aaaataaaat aatgttatag tatattaaat accttgaatt caaccatttg ttgattctaa
     5341 gtaaaatata acttccatat aatactgtaa taattgaaga gagtattacc ttcgggtcaa
     5401 tgaatatacg ttcaccaact gaaattacac cccattgtgt acctaaaata atactaaata
     5461 tgagaattat ccacccactt aacgttgaat aaaacacaat tgattcaagt gtagcaacgc
     5521 tacctattct aaagtatttt tgatcaaaac gcttttcctt caaattacgg tattgcatga
     5581 tatacagtaa tgcattgaca aaagctaagg caaagaagac ataacttaac acagctagac
     5641 caatatggac taacagtaac tcgtctacaa cagcaatttt ctgaacctta ttagtataat
     5701 gtgtcggttg aaatgtattc atccctaaaa gtgttaaccc tattaaattc aagaaaaaca
     5761 cagagaaatt caaaactttg ataaggttaa gaattaaaga gattgaaata atcagccaac
     5821 ttaaagtata gaatacatct gaaatagacc ctaatggaat atgtttagtt tgtataataa
     5881 aaatagataa agagattgtt tgtaaaaccc aaacaatccc caataaatat atgcctaaac
     5941 ttctaatctt atgacttttt tggacaaaat cataaaaata gcaaatgata ctgattaagt
     6001 atattaataa tataatttca ttgaatcgaa taaacaggtt ttcttgcata ttatcaccat
     6061 attaatatgc ttattcaaaa ctaaagatac gtcgcgctga aatttccttg accttgcttt
     6121 ctttttgttg cttcgcttgc tcatgaggac atttagcttc gatatcaaat atattttgaa
     6181 atagctctaa tttttcatta cttttcttat cactacttaa ttctttggcc tgtttaatag
     6241 gatctttcaa catttgattg atgatacttt ttgtatgttt agatataatt ttacgttctc
     6301 tttcgcttaa ccctggcaat ttacgatcaa tactgtccat cgtttccgct tgaattgcca
     6361 tagctttttc acgtaaagct ctaatcactg gaacaacacc caacatacta acccactcat
     6421 tgtgtgcatg tatttctgca ggaatttgtt ccgaaattgt tgcagccgct aattgtcgct
     6481 cacgtaagtt tgcatcaact aaacctttta agtcatcaac atcataatta aagatgtttg
     6541 tgatggcact aataccaggt tcaatatctc gaggaactgc aatatcaatc aatactagtg
     6601 aatcttgctt tctattttct gcaattcttt ctatcatttc atttgtaatg atataagatt
     6661 gtgcactcgt cgaactaatc acaatatctg cactttcaag taaatttggt aatgatgata
     6721 gttcatcata tttcacttga tgctttgctg ctaatttcat agcattttca attgttctat
     6781 ttactactgt aatatcagta attccagaac caagaagatt taatagtgat aattcactca
     6841 tttcccctgc accaataatg atagcttgct tacttttcaa tttgccaaat acttttttcg
     6901 ccaactcgac cgcagcataa gacacactta cagcattatc agctatatct gtttcattat
     6961 gtgctctttt tgcaaaagta attgcctgtt taaatagatg attaaaaatt gttcctgtcg
     7021 tacctgtgct ttgcgctaag aaaaatgcat ctcttatttg acctaaaatt tgagtttctc
     7081 caagtacgat tgaatctaaa ccagaagtga cacgcaataa atgttctact gcttcgtccc
     7141 ccacttttac ttctgacatt gctttaatat catctacttc aaatccaaat gcacgagcta
     7201 gaaatcgttg aatatagtaa cgacctgtgt gaatttgatc aacaacagca tatacttcag
     7261 ttcgattaca tgttgataat atgacatttt ctaaaataga tttagtttca tataaatctt
     7321 catgggcaat tcgtaaggca tcatctctaa aagcaacttg ctctcttagt gcaacatcag
     7381 ctgtgcgatg atttatacta attgcaataa aatgcatttg aaacgccccc atatatacta
     7441 acaatacaaa tattataaca taatttaaaa tccaattaag tacgatgctt aaataaatta
     7501 taatctttct aatttacttt tgagtttttg acaaaatgat gaacgttgaa aaatgttatc
     7561 aattgtgtac taattaaaag tttcgtgctt acattatttt taaaattttt gtataagttt
     7621 tgtcgtacaa aaactatgaa atatacggtt caattaaatt ccatatttgt tgttgtttat
     7681 tattttgaat tgatgaataa cttacaattg tatcgtctgg gtccatatct aattgtgtct
     7741 taatattttt aatatgcttt tgaactttgc cttttggaat tttgtcttct ttagtgcata
     7801 taactaaagt aggaatatca aaatgtttca aataattgta cattaagata tcatcttgtg
     7861 ttggatcatg tcttaaatca actaattgaa taactaattg taaattctct ctcttagtta
     7921 tatattcctc aatcattttc ccaaattttt cacgttgtgt tttacttact ttagcatatc
     7981 cataccctgg aacatccaca aaaataagtt gttcatctat attataaaaa tttaacgttt
     8041 gcgttttgcc gggttgctgt gatgtacgtg ccatattttt tctgccaatc atactattga
     8101 taaatgtaga cttacctaca ttagatcgac cgctcagtgc aacttcagac aattctgttt
     8161 ctggatattg atcttccttt actgcactaa tgattaattc tatattatta ggattaactt
     8221 tcattttata tcctcacttt tttatattct ctttcaactt taccattata taatatttga
     8281 acctttcggt atacattata aaaaagctcc gatcaaagtt aaactatttg agcgtcaact
     8341 ttgattggag cttttcactt ttataacaca tcaatgatta agctgatgtt ttactattat
     8401 taattaaatt accttctgcg tcgtataatt ctggttcagt ttcttcatta attgtttgtg
     8461 ctgtaataac taccttcgtt acattttcgt tagaaggcac atcaaacata atatcgatta
     8521 acgattcttc tatgattgaa cgtaaaccac gcgcacctgt ttttctttca attgcttttt
     8581 cactaattgc tgataaagct tcttcagtga actctaaatc tacatcatct aattccagca
     8641 ttttagtata ttgtttcaca agtgcatttt taggttgcgt taagatgttt ttcaacgcag
     8701 ttacatctaa tgtttctaaa ttagctacaa ttggcacacg tccgataaat tcaggaatca
     8761 aaccataggc ttgcaaatct tctgggcgaa tttgtgctaa taatgcttgt tcgtcatatt
     8821 tatcagcttc attgcttgag aaaccaataa ctttttcacc aagacggcgc ttaatcactt
     8881 cttcaatacc atcaaaggca ccaccaagaa taaataagat atttgttgta tcaatttgaa
     8941 tcatttcttg gtttggatgt ttgcgtccac cttgtggcgg aacacttgca gttgtacctt
     9001 ctaagatttt aagcaatgct tgttgaacac cttcacctga aacgtcacgt gttatagatg
     9061 tgttttcaga tttacgtgca attttatcaa tttcatctac ataaataata cctttttcgg
     9121 ctttatcaat gtcaaagtca gctgcttgaa ttaatctcaa caagatattt tcaacatcat
     9181 cgcctacata accagcttca gttaaacttg tcgcatctgc aattgcaaat ggtacattca
     9241 acgtcttggc taatgtttga gctaataatg ttttaccact acctgttggc ccaattaagg
     9301 caatgttact tttttgtaac tcaacatcat cttcttttgg tcctaattgt tgaatacgct
     9361 tatagtggtt ataaacagcc acagctaaag attttttagc tttttcttga ccaataacat
     9421 attcgtttaa atgatccata atttctttag gagtaggtaa ttctgtaatc gcttcagaag
     9481 tgttttgagc taattcttct tcgacgattt ctgagcataa ttcaatacac tcattacaaa
     9541 tatatacacc acttcctgct acaagttttt ttacttgatc ttggtctttt ccgcagaaag
     9601 agcatttcaa attttcttca tcttcattga atttaaacat tctttttaca cccctattcg
     9661 ttaaagacta tactagattg gatattacaa tgcaacatat taacatacaa actttttgct
     9721 taaagaatag tagcagatac ataagctatg catctgctac tcatttttgt aatttaatat
     9781 ttaatgaaga ttaatcttct ttagttcctt caacgaactt tgcgttatct cttaataaat
     9841 cgataacttt ttgaatacga acatcatttt taatgatatc agtattacct aaagtatttt
     9901 tgatatcttc aactgagata ttaaattgtt tactcatttt ttctaattct ttatcgatat
     9961 cttcatcagt agcttcgatt ttttcagctt cagcgattgc agttaaagtt aagttagttt
    10021 taacacgttg ttctgcatcg tctttcattt gctctcttaa ttgagattca tcttgacctg
    10081 agatttggaa gtacgtttgt aaatctaaac cttgttgttg aattctttgt gcaaattcag
    10141 acaccatacg atctaattca gtattaacca ttgcttcagg aatatcgatt gttgtattat
    10201 cagtagcttt agtaatcgct tcttcttttt caacattttc tgcatctgta gctttttgtt
    10261 cagctaaacg tttacgtaag ttttctttgt actcgtctac tgtatttgct tctgcatcta
    10321 attcattagc aatttcatca gttaattctg gaacttcttt aaatttaatt tcgttaactt
    10381 ttgttttgaa agttgcttct ttaccagcta attcttcagc atggtattct tctgggaatg
    10441 ttacgacaac atctttttct tcgtcaactt tcataccttc taattgctct tcgaaaccag
    10501 gaatgaatga acctgaaccg atttctaaat cgtaaccttc agcttgtcca ccttcgaatt
    10561 cttctccgtc aactgaacca ctaaagtcga tgttaactgt gtcgccattt tcaacaacac
    10621 catcttcttt aactaccatt tcagctaaat gtcctaagct gtggtcaatt gcttcttgta
    10681 actcatcatc agataattca gtttcttgtt tttcaatttc aagaccttta tagtctccta
    10741 atttaacttc tggctcaact gtaactgttg cttcaaaaat gaaatcttta cctttttcaa
    10801 tttgagtaac acttacttct ggttgtgcaa ctggtttaat atcagtttcg tcaattgctt
    10861 caccataagc atctggtaat aaaatgtcga tagcatcttg atataatgct tctacaccaa
    10921 agcgttgttc gaaaattgga cgtggcactt tacctttacg gaatccaggt acgttaattt
    10981 gtttaaccac ttttttaaat gcttgatcta acgctttgtt tactttttct gcaggaacag
    11041 taactgttaa taaaccttcg ttaccttcct ttttttccca agttgctgtc atgtatatat
    11101 acctccatga ttaactaatt tattttttca acttccctat tatatcatag gtctattccc
    11161 tatacaaaca ttgaaatcac aacgtttata tatttgtaaa tcaacttttt tcgtcaaaac
    11221 tatattaaaa tgctgagtat tattttgcaa caatttaact attattattc aaatctaatt
    11281 gctgaataaa attaaatgca tcgatacttt gtaattcaca tttaagtcct aacatatttt
    11341 tgaaataaca ttcgtatgca ttaatccatt tattcgtttc aaacaaagtt tcaatatcaa
    11401 ttggataaat catgatagag tgattattca taatatggtg tgcttcttca gcgatatgta
    11461 aagcaccttc atttaatgtt tccataacgt taggtataac tttttctttc agtgttgtat
    11521 gttctagccc ttttaaatta gctggtacaa aagttatatc cataccatac ttttcaattg
    11581 tcagttcttg tgtacaattt gcgaacctta aatactcaat cattaaacta attagtttat
    11641 aactgtacat attagatttc aatatatata ataccgtttc ttgaaattga aaatgaccat
    11701 tgtctattaa cttcaaaatc aagtgcgtct gttccctcat tgataacgta tcaaaatcag
    11761 ttaatgactg tgtcaatcgt ttttcatctt caattaattt tgacttagca aattctttaa
    11821 gaggatgtaa tgccattctt gttttgtgat ctttgacctc atcaataatt tgatgaatta
    11881 cttctaccgc ttcaaaatat tgttccaaac ctatcaaact tttcacataa taaatcatca
    11941 atgcatcata ttgttgtatg ccagttttca ataagacaat tgtttcttct cttaattcaa
    12001 gaaatgaccc cgtctcatat aacatacggc atttcatcat tgctatttct tcagtgagtt
    12061 caaattgacg ttcatattgt acaatatatt catacatcag gtcatagttt tggtcttgtt
    12121 cagcacgttt tatatcttta tataactttt tcgaagagtt aggaaactga ataatatctg
    12181 acatatatgt cacctcgttt gattataaat cttgaaggat taatgcgctc cacgtctcga
    12241 aatcatcata tgactctatg ttgccttttt caacccattt tattcgtaaa gtatctgttt
    12301 ctttagcttc tacatcattc gtacttacag tgattttaaa tacaacacca atatgtacct
    12361 tgcccacttc gttattatcg tcattaataa aaccgatata ttccatattt tgtgaatctt
    12421 gctcacttaa acctacttct tcttctaatt ctctctgtgc attaactctc aatacttcgt
    12481 taatagattc tgctcctgga acatcattca tatgaccgcc tacacctatt gaagattgtc
    12541 catgcaatcg agcttctcca ccgccagata atcgttcata cactaatatc tcgccatgct
    12601 cattttcaag taaacaataa gaaatgagtt gtttatatga tggatcctct tccatatcgc
    12661 cgcgtcgctt aacttcatat tgacttagcg catcaaaaat attttgacct tctagtttat
    12721 tcttatttaa aaacccattg aaagtattct tttcattatt aaaaataatt tctctaggta
    12781 ctacaatgat ttgttcatca aatttagaca tcgcataaaa ctcctcactt taatgtttgt
    12841 tctcttaaat gcattttagc aaatatgtca tattttagcg attaaatgtg gcattttgct
    12901 atttaaaaaa taaaaaagag atgacaatgc catctctttt ttataagtgt tattatttta
    12961 aagcatcttt agctttagtt actaattgag caaatgcttt ttcgtcagaa attgcaattt
    13021 ctgataacat tttacggtta atgtcgatac cagctttttt caaaccgttc attaaacgtg
    13081 agtagctcat ttcatgttga cgagctgctg cgttgatacg tgtaatccat aatttacgga
    13141 agtcacgttt acgttgacga cggtcacgga aagcgtattg acctgatttc attacttgtt
    13201 gcttagctac tttgtataat gtatgttttg aaccgaagta acctttagct aatttaatcg
    13261 tttttttacg acgcgctctt gttactgttc cacctttaac tcgtggcata aaaaattcct
    13321 ccttaggtaa ttcctaccga tttctgtatt tgttcttatt ttttgtatgc taataattgt
    13381 tttacacgtt tcatgtcgct cttagacact aatctagctt tacgtaattg acgtttttgt
    13441 ttagtgctct tgtttgcgaa taagtgagat gtgaaagctc ttgaacgttt taattgacct
    13501 gaagcagttc ttttaacacg tttagctgct ccgcggtgag ttttcatttt tggcataata
    13561 taagtcctcc tgtaatagct ttaattattt ttcagctgtt ggcgctaaca taataaacat
    13621 ttgacgcccg tccattttag gtttttgttc aactgttgct atatctttac attcatctgc
    13681 atatttttct agcacacgtt gaccaatttc cttatgcgta atggcacgcc ctctgaaacg
    13741 aatagatact ttacatttat cgcctttagt taagaattta cgtccgtttt tcaacttagt
    13801 ttggaaatca tgttcctcaa ttgttggact taaacgaatt tctttaacat tgataatttt
    13861 ttgtttcttt ttcatttctt tttctttttt ctgttgttcg aatttgaatt taccgtaatc
    13921 cataattctt gcaactggtg gtttcgcatt cggtgcaacg accactaagt ctaaatctac
    13981 acgttcagcc atttctaaag cttcacgctt tgatttaaca ccaatttgtt caccatcttg
    14041 actgattaaa cgtaattctt ttgcacgaat tttgtcattg atttgagttt gatcttttgc
    14101 tatggttgac acctccaaaa tttttacgaa atttgcacca agcaaaaagg aagagcaggt
    14161 ataaaatacc cgctcttcct aatacacagt tatgtgtaat gtgattaacc tgccaactgc
    14221 tttatgcgtc gctacaggtg agaagcgggt gcttctactt ggttcgtttc gtattcaacg
    14281 ttgttaatca tatcaacaat tcacatttaa gtcaacacca taactgtaat tatttttatt
    14341 ttaacctttt atttcatcca ttgacacgtc ttgacgtaaa tctacttgtt ctaatggaat
    14401 tttcttcgtt ttatatcgaa gcttatgata aataaagaat gctaaaaata ctgggattcc
    14461 catatacgta attaagaagc gactaaaatt aaaatctcct gttttaataa agtcaacatc
    14521 ttgaccgatg attacaacaa tacataaaat agctgcaaaa ataggtccta acggaaacca
    14581 tttagctgta tatttcaatt tagatttatc ataattttgt ttatcaaatg cccttctaaa
    14641 tcggtaatga ctcactgcga tacctaccca ggcaataaag ccagttaacc cacttgcagc
    14701 aacgatgtat tgataagcac cttctgtaac actttgtacc acaaatatga tgactacaat
    14761 aattgcagtt actaataaag acatatatgg cacaccattt ttatttgttt tcccaaatgt
    14821 ttcaaacgcc aatttatctt tactcattga ataaagcata cgcgttgagg catacattcc
    14881 agagttacct gctgataaaa ctgatgttaa gataacagca ttcataaatg aggctgcaaa
    14941 agcaaatcct gcatttttaa aaactagcgt gaacggagag gttgcaacgt cattaccgcc
    15001 tcccattaat gcactactat cataaggaat taacataccg ataacaaaaa tggctaaaat
    15061 gtaaaataat aaaattctcc agaatacttg tttaatcgct ttcggcacag cacgttcagg
    15121 attttctgat tccccagctg taatacctat taactctgta ccttggaatg aaaaaccagc
    15181 aattaagaat acacctaaaa tagataataa acttccgcct agatttccac caagaatagg
    15241 accttcacct ttattaaata tttcgaatcc tacaacatga ccacccatga ttccgacaat
    15301 cgttaataaa ccaattgcaa tgaaaacaat aactgtaacc acttttatca atgccaacca
    15361 gtattcactt tcaccataga cgcgaaccga taacgaattc agactaaaaa ttataactaa
    15421 gaacaacgca ctccatgccc aagctggtat gccttgtaat ggtgtccaat attgaatgac
    15481 ttgtgctgca atcgtaatat ctgctgctac agttactacc cagttaaacc aatagttcca
    15541 accaagcgca aaccctaaag atggatcaac aaatcttgta gcatatgtac taaatgagcc
    15601 tgatactggc aaataagtag ccatttcgcc aagtgacgtc attaagaaaa ataccattat
    15661 tccgataatt gcgtatccta ttaatgcacc caaagcacct gcatcatgaa ttgctccacc
    15721 agaagttaca aataaacctg taccaataca acccccaatc gcaatcatag aaatatgacg
    15781 atctttaagt ccccttttga caacattgtt actttcattt tgaacttttg acatataagc
    15841 tccctttcca tgttttagag gccaagtaat aaataacctt tatttacttt catacccttt
    15901 ttttacagaa tatagtgata ttaaaagtaa aaaagtggct acataccttt aagatatgca
    15961 accactttgg caattatcta tataaggtag cacatcacat tctgtgacag tacagttcct
    16021 attcgaaaac tgtcccaata gatataattt atggtttata tctatttcgg catctttacc
    16081 tttcacttgt tcaacttatg taccataaat acttctgaca agttactaat taaacatgca
    16141 acctctaact caatttaata ttttaactaa cttgtaatat acaggattca tcacgcataa
    16201 tcaaccctgt aaaacttgat acgcaataaa agtttcaaag cattttattg cgacaactgt
    16261 ctatctatgt tttttcaaac gaatttcatc aactagattc cagataaatt catctttttc
    16321 aactgtttct tggtcttgtg atccatattg acgcacattc acttgattat tttcaacttc
    16381 cttatcccca actacgattt gataaggtat tttttgcatt tgagcttctc taattttata
    16441 acccattttt tcattacggt catcaatact tacacgaacg ccttgagatt tcaattcatc
    16501 ttgtaattgg cgcgcataat cataatgtaa atcaacgtta actggaatga tttgaacttg
    16561 ttttggcgct aaccaagttg ggaatgcacc tttagtttct tcagttaaaa atgctacaaa
    16621 tcgttccatt gttgatacaa caccacgatg aataacaact ggacgatgat gttcaccatc
    16681 ttgaccaata taagttaaat caaaacgttc tggtaataag aaatcaagtt gtgctgttga
    16741 taatgtctct tctttaccca tcgctgtttt aacttgaaca tctagtttcg gaccatagaa
    16801 tgccgcttca ccaatcgctt cttcgtacga taagccaagc tcatccgctg cctctttaag
    16861 catattttca gctttattcc acatgtcatc atcatcaaag tacttttctt tatcttcagg
    16921 gtctctataa cttaatctaa agctataatc ctcgaaacca aagtctttat atacatcaat
    16981 aatcatgttt acaacgcgtt tgaattcttc tttaatttga tcaggtcgaa caaagatatg
    17041 tgaatcattt aaagtcatac ctcgaacacg ttgtaatcct gatacagcac cacttgcttc
    17101 atatctatgc atagttccta gctcagcgat acgtataggt aattcacgat atgagtgtgg
    17161 tttattcgca taaatcatca tatgatgtgg acagttcatt ggacgtaata ccatagattc
    17221 agtttcatct aactgcattg gtgggaacat atcttcttga tagtgatccc agtgaccaga
    17281 tgttttgtat aaatcaacat tagcaagtac gggtgtataa acgtggtcat accccatgct
    17341 aacttcttta tcaacaatgt aacgttcaat ttcacgtcta attgttgcac cgttaggtaa
    17401 ccataatggc aaaccagcac caactaattg gctatttgtg aataattcta actctttacc
    17461 aattttacga tgatcacgtt ctttacgctc ttctaacatt tgtaaatgtg cttttaattc
    17521 ttttttatca aagaaagcag taccataaat acgttgtaac attttgttgt tactatctcc
    17581 acgccagtat gcacctgctg tagataatag tttaaactct ttaattttag ctgttgatgg
    17641 aacgtgaact ccacgacata aatcagtaaa atcaccttga ctatataatg ttacattttc
    17701 atcttcagga atcgcgtcga ttaattctaa tttgtattca tcattgctga ataactcttt
    17761 cgcttcatct cgtgaaacca cttttcgttc gattttcata ttttcgttaa cgatttgttt
    17821 cattgttttt tcaatttgtt caaagtcatc agatgagatg ttttggtcaa tgtcgaagtc
    17881 atagtagaag ccaccttcta ttacaggacc tacaccaaat ttaacattac catataacct
    17941 tttaatcgcg tgtgccatta aatgtgcagt agaatgacgt aatacctcta acgcttcttc
    18001 actacctggt gtcacaattt caattgatcc atcagtttca agcggtttag ttaaatctac
    18061 aagttgcccg ttaaatttgc cggcaacagc ttttttacgt aatccaggac taattgattg
    18121 tgctatatct tcagtagtag tacctttatc aaacgccttt ttattaccat ctggaaattg
    18181 aatattaatt tgttccatgt taaaccctcc tatttgcttt ataaatactt ttatctgacc
    18241 tcgttaaaaa attgaacaac aatcatcgtt ttaacattaa acctattaac aataaacaga
    18301 gaatttatga caccaacaaa catttatgtc tgaaaaaaac aaaaaatccc gcccctatac
    18361 aagggacgag atcgtcgtgg taccacccta gttatttaat acaagttatg tattaaatat
    18421 ctctacttaa gataacggtc ttgatccggg tattcattac aaataccata aatgaagtag
    18481 taatcatcta atttattaac catattctca tcaaacaatg gctttctgtg taatattgat
    18541 tagatcatct tgtcttcaat catttaaacg atttagattt gtattcatta tacaccaatc
    18601 attttaaaat tcaattgttt ctgaaatttt ctcctgataa aaagtatggt gttgacaaag
    18661 atttgacacg ttcaataata cgtgctgctt tagtcttctc ttcaccatca cgagtcatcg
    18721 ctaaatgatg ttccaattca ctatagtcaa aattagaact aaagaatgtt ggtaattcat
    18781 gaaccattcg ataatgtagc aaaggtccaa ttacctcatc tctcacccat ggagtcactt
    18841 cttcagcccc aatatcatca agcattaaaa tgtttgcttc tcttacgcga tgtaatttct
    18901 tttcaaaaga accatcttta aagccacctt ttaatgttct aataaattcc ggtaaataaa
    18961 taattgtcga acgtaccttc ttagatttga gctgattcgc aattgcacct agaataaaag
    19021 atttacctgt cccaaatgga ccataaaggt aaaggccttt cacttgttcc ccattagtta
    19081 ttgctgtaca aatatcatct gctgccatag ctacatcaag acggtctcga tgattcatat
    19141 aaatatcttt caatttggcg tttaaagtat ctcgttgcat atgatgagat gtaattagct
    19201 cagcttcaaa gcgttcttcg tcgtacttga ttttacatgg acattgtaaa tagcgtattt
    19261 taattcggtt attatcaaca tataactcag gcacatgccc ctttacgaaa tttggacaat
    19321 cagcaaattt atgaccgtca taatgttttt gttgatcttt atactcttgt aacacattta
    19381 agtcttcatc aatcatagca ttcgttaatt cagctcgatg cgcttccaaa aattgcttaa
    19441 catctgggtc attgattact tcttttttta tcttttctat tcttttttca aagtcctgcg
    19501 acgtgttaat tatactttta aattgcttca ttattgactg tcctcctccc attttttaga
    19561 taatttatct agaaatgctt gtcgatcttg ctctaattgt tgatcatcta cgctattatc
    19621 tttagccgaa tcttgttcac taggtttatc tctattttct aaccatttag gtgttttttc
    19681 ttttgaaatg cgattacgct gcccatagta tgaaccacgc ttttggtaat ttccgctaga
    19741 accctcattt ttaggttgat taactttttt agcgtaatta tatgcttctt tagctgtctt
    19801 aatacctttt ttcttccaat ttgatgctat ttccaaaata tacgctttag gaagtttcat
    19861 atcttctttt aacatgacaa attgcaacaa aatattaatg acaccaaaag acattttttc
    19921 acgttcaatt aattcttcaa ccaatgtctt ttgcgatata gttggttctg attcagacca
    19981 agaagctaac atatcaattg gactcgtttg ttcaagtaat tcaaaccatt catcactttg
    20041 tggctttgga ttcgcttctg tagatttggc cgtcgaagat gatgtagcag gagatttcac
    20101 ctgtaattta ggcatttgat tttcgtgttc cattaagtaa tacgagcgtg cttgtttacg
    20161 catttcttca aaggataact gttgtccact tgtaattgaa tttaaaataa catgcttcat
    20221 gccatctgct gttaaaccat ataaagtcgc gagttgtgta attaaacgct ttgcatcttt
    20281 ggtaacaatg tcttgactaa taaaatgttt acctaacatt tgtctcaaca tttcaaaatc
    20341 aaaagattca tttgataaat cgattccttg gtacggttca ttaatcggaa tatcacttgt
    20401 atcgatatct atttttgtag acggcacttt aaaaacatca gtaaattgtc tcgttacctg
    20461 tttaaattca ctcaaatcaa tttgttggta ctcaaagtat ttcttcaact catgaaatcg
    20521 acgatgctcg acttcactat ataaaaagat tgacaacatt ggatcattaa aaaataaatg
    20581 tgctgaaggc ggttgaatta attggtaaac aaattgtgtt tcttgttcat catgtttgac
    20641 aaacgccttt aacaatccaa tcgcttcaag taagtccatt tgttgtctaa actctagtaa
    20701 attaatttta agttcattca taaaaatata atgagaaaga atcaatgttt cattatgact
    20761 ttctttaacg aattgagtca taaaatggta taaacccact gcttgtgttc caattagcgg
    20821 tgtatacagt cgattcaata cctctaaatg attcgtattt aaatcaaagt gttgcataac
    20881 tttgaattga tcctttggtc ttaagccgaa ttcaaaggct tgtcgtccca tttaagcatc
    20941 actccgtttg ttttcgctta aaatcccttg catcgatgct aacaattgat caacatcttt
    21001 aaattcttta tagactgatg caaatctaac atatgaaact tgatcaacat gcattaacaa
    21061 gttcataacg tgttcaccta tatctcgtga agacacttcc gtatgacctt catctcgtaa
    21121 ttgccattca accttgtttg ttatgtcttc aagttgttga tatctaactg gtcgtttctc
    21181 acaagaacgc acaagtccat taagtatttt ttctcttgaa aactgctctc ttgtgccatc
    21241 ttttttcaca actataagct gactaacttc gatatgttca aatgtagtga aacgtgttcc
    21301 acaattttca cattctcttc gtcttcgaat ggcatttaat tcatcggcat gccttgaatc
    21361 tacaacttta gattgtgtag aattacattt cgggcatttc attacatcac cctctttatt
    21421 ttgattatgc ctaattatac tataaatcta gagatgaaaa aagaatccct caatttaatt
    21481 catttaacca aataatgaaa caataaaaaa cattatatcg ttacttatta agtaataagc
    21541 atgacaatat tattatatta aaaataaaaa acctaactcc gaggtcagag ttaggctata
    21601 aattaattgt attaacttgc acttacagtt tcttttgatg tcaaaagtgc tccaatttgc
    21661 tcagcgacat ctacaactct atttgaataa ccccattcat tatcatacca agcaataact
    21721 tttactttat tccccgacat aaccattgtt gattttgcat caataatagc tgaatgtgga
    21781 ttagtattaa aatcaacaga cactagtggt tgatgttcga cttcaatgat accttctaaa
    21841 cctgcatttt caaaagcttg gtttacttct tctgcagtta cttctttttc taaatcaaca
    21901 actaaatcaa cgagcgatac attctttgtt ggtacacgta atgccatgcc gtgtaattta
    21961 ccttctaatt ctggtaatac ttcttttaaa gctttcgccg caccagtaga agtaggaata
    22021 atgctttcat tacatgagcg tgcacgtctt aagtctttat gtggattatc aatatttttt
    22081 tggtcatttg taatagcgtg aacagtagtc attaaaccat taactattcc aaactgatta
    22141 tttaaaactt ttgcaactgg accaatgcaa ttagtagtac atgaagcatt actaaaaatg
    22201 tcaaatgctt ctatatctaa ttggtcatca tttacgcctt taactaccat ttgaacatgt
    22261 ccaccttttg aaggaccagt taacaatact tttttggcac ctgctttaat atgtgcgatg
    22321 gctttatcac catgattaaa tttaccagtt gcatctatag caatatcgat atctaattct
    22381 ttccatggca agttttcagg attgcgatca gcaaccaatt taattttgtg atctccaact
    22441 tgcaatccat tttcaatcgg ttctactttt agattatatt ttccatgtgt tgtatcgtaa
    22501 ttgattaaat gtgcaattgt ttcgggtgga taactagcat ttatcgctac tacatttaaa
    22561 tttttatttt gtaatgcaat acgtaatacc attcttccaa ttctacccat accattaatt
    22621 gcaatattcg ttgacattaa aagcacccct tgatttttat gtgttatact ttatggaatt
    22681 agtatagcat attatatgaa atttgaaagc gttttccgtt gatttattta tcttttttat
    22741 aaaaaatagt cattacttac atacatccac gcatacgtaa agtaatgact tatttataga
    22801 gtaatattaa tctccttctc cgtaattcgg cttttcaata taaccttctt cttctaacaa
    22861 tctctcaagg ttttgtttta attcaagttt atcccctaaa ttatcgataa catgatcggc
    22921 cattcggctt tttttatcaa tagaaatttg gctatagaca cgagcttttg catcttctaa
    22981 tgacaaatta ttacgttgca ttaaacgatc catttgtata ctttcagaag tgtatacaac
    23041 ccacacttcg tctactgtat tttccaattc attttcaaat aataatggaa tatccatgat
    23101 tacattatat ccttgtttta aatattcttg cttttcttct tccataatat ctcgcacgat
    23161 aggatgtatg atagcattta attctaagcg tttttctgga tggttaaaca ctaaatcacc
    23221 catataacga cgattcatct caccattttc atcaattgct tcatcaccaa agacttctcg
    23281 tacttgagct aaacctttac tccccttttt aacagcttcc ctggctgctt tatcagcatc
    23341 tactacttta aaaccgaata cggataagag ttctgatact gttgattttc ctgaggcgat
    23401 tccacctgtt agaccaataa ctttcggcat aatttcactc tttctttatt tttgacatac
    23461 tggacaataa tgactatttc ttgtcgcgat gatttttgtt tcaatttgac ttccacacac
    23521 tttgcatacc ggctgcttat atacattgag gtgcaattgc atctcaccag tttttccatc
    23581 agcatgacga taatctgaaa tacttgtacc gccatgctta ataccttctt ctagtacttc
    23641 tctaacataa taaaaaacca tttcttgttg ttggtgtgtt aagtctttta cttttttatc
    23701 tggtaaaaca cctgcacgaa acaacgcttc acatgcgtaa atatttccgc aacctgcgat
    23761 tactttatga tccaaaatca cttgtttgat tggtttattc ttattagact gttgatgaat
    23821 tcgatttaaa taatacgtca atgcttcatt tgtaaaaggt tcaggcgcta tttccagaaa
    23881 tgaaggataa gatgctacag acgcaacatt tctaatttct ccaaaacgac gtatatctga
    23941 ataaattaac tttttgtcat ttgacagctc aaaaataaca tgccaatgct tacgataatt
    24001 aggtatcata atatcttcaa gttcatctac aatgaaaaaa ccacccgcca tacctaaatg
    24061 gctaattaat gtacgttgtt ctcgtttatt atctagttga aaaacgatat atttacttct
    24121 tcgttctaca ttagtaatgg tatagcctta cgataaagtt ttaaaagtat ctaattcaat
    24181 tccttttata attgtttcct tgccttgagc tttaccttcg attactttat ccgaaaatat
    24241 aacgtgttca attttctgat ttataacgta gggttcaatt cctcttttta catgttctac
    24301 ttctggtaat tcgggcacac cattaacctc actttatttt gcatcatacc aagttgcacc
    24361 ataacttgag tctactttta atggaacatc taattgcaat gcattttcca ttatctcttc
    24421 tacaaattca ctaaatgaat ctacttctga cttaggtact tcaaaaatta attcatcgtg
    24481 tacttgtaat aatagtttag cttgatatgt tgtctctttc attttttgcg caaatttaac
    24541 cattgccagt ttaatgatat ctgcagcact gccttgtatt ggcgtattca tagcagtacg
    24601 ttcagcaaag ccacgtaaat taaagttacg actcgtaata tcaggaatat agcgtcgacg
    24661 atgtagcaat gtttccacga aacctaaagc tttggcatct tttacaatat cagacatata
    24721 ttgttttaca cctgggaaac tagctaaata atcatcaatg aatgcttttg cttttttacg
    24781 agtaatacct aaactttgac ttaaaccata atcacttatc ccataaacaa ttccaaagtt
    24841 aaccgctttt gcttgacgac gcattaaact atcgacttga tcagcttcta caccaaatac
    24901 tttcatagca gttgctgtat gaatatcatc gccgttgata aatgcttctt tcatactctc
    24961 atcttgtgta atgtgtgcta atacacgcaa ttcaatttga gaataatctg ctgataatat
    25021 aacgctatct tttgaagttg gtttaaaggc ttttcttatt ttacgccctt cttcaagtct
    25081 aaccggaata ttttgtaaat taggatctac acttgataaa cgtccagttt gcgctaaagt
    25141 ttggttaaaa cgtgtatgga tacgttgatc atcactaatt accttttgca atccttcaac
    25201 ataagtagac tgcaacttag ataattgacg atattctaaa atataatcaa tgataggatg
    25261 ttcaccttgc aattgctcta agacatctac agcagtagaa tatcctgttt tcgtcttttt
    25321 aataacaggt aattgtaatg tctcaaacaa tacaacacct aattgcttag gagaatttat
    25381 attaaaatct tcaccagctg catcatggat atttcgaatc aagacgtcta atttttcttg
    25441 aatttctttt tccatttctt ctaaatcatg aacatctgta tatataccaa tttcttccat
    25501 ttcacttaaa atcttagcta gcggtagctc taaatcagct aagagttcta cctgattgta
    25561 ttcttctaat tgtttatcca tatttggttt cgcaaagtaa attgcatcag taatagaagc
    25621 aacatatgga tttaaaacat catcttcagg taccttaaat ttcttacctt ttccatatat
    25681 actcacatcg tctttcacaa aactttgacc gtacaatgaa acaactgatt gaacatcact
    25741 aatcgtacga gatggatcaa ttatataact ggccaacata atatcgaatg aaatattttg
    25801 aatatcaatt cccaatctat gtgatgctac atatgttttt ttagcatcat atacgacttt
    25861 tttcgtattc ggattttcta accatgaaac tagttcagca taattaatta tgtcatccgc
    25921 attaattaca atatgtttct cacctgtaaa taaagagaat tttaaaatat tatttcgcaa
    25981 atagttgcca ccgtctaatt cgaaatggat ggtcgcttct ttcaatgaag taaaatcaac
    26041 attatcaaaa gacgtttcaa tttcaaatgt cttttctatt gcatcttcac cacttgctga
    26101 ttgatcaatg tcagccaaca attgtttgaa ttctaacttc ttaaacaatt cgattttttc
    26161 ttgttgttca tcttgatgag tcattaacgt atcttcaagt tttacttcaa tcggactatc
    26221 tacattaatc gttgctaatt ctttactcat taatgcatct tctttgctgt tttgaagttt
    26281 ttcttttaac tttttacctg aaatttcatc taaatgttca taaacacctt ctactgtgtc
    26341 aaattggttc agcaatttta ttgctgtttt ctctccaaca cctgcaacac ctggtatatt
    26401 atcagaagta tctcccatta atcccttcat atcaataatt tgattaggtg ttaacccgtt
    26461 gtatttttca gcaataaagt caggtgtata atgatcaaca tcagtaacac cttttttagt
    26521 gtaataaatg gttacattat ccgttgcaag ttgtgttaaa tctcggtctc ccgtaataat
    26581 aattgtctga aagcccgctt tatctgcttc tttacttaaa gttccgataa tatcatctgc
    26641 ctcatagtta tctaattcat aacgtttaat atgataagca tctaataatt ggcgtatata
    26701 aggaaattgc tcacttagtt caggcggcgt tttctggcgt ccacctttat attcactata
    26761 tttttcatgt ctgaaagtcg ttttacctgc atcaaacgct actaaaaaat gatttggctt
    26821 ttcttctttt aaaatcttct ctagtaacat tgcaaaacca tatactgcat tggtatgaat
    26881 gcctgctttg tttgataaca aaggtaatgc ataaaaagct ctaaaactta agctattacc
    26941 atcgattaat actaatttat tcacaatttt aacctccaga actaatttat atatatttta
    27001 gcatattcga ctgtgatgtc gtaattaaag aaac
//
LOCUS       DAKSQE010000025        26386 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.025, whole genome shotgun sequence.
ACCESSION   DAKSQE010000025 DAKSQE010000000
VERSION     DAKSQE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 26386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26386
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.025"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(724..1356)
                     /locus_tag="O0V33_000914"
     CDS             complement(724..1356)
                     /locus_tag="O0V33_000914"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCX8975576.1"
                     /translation="MTNYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQRVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVCEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW"
     gene            complement(1353..2111)
                     /gene="hisF"
                     /locus_tag="O0V33_000915"
     CDS             complement(1353..2111)
                     /gene="hisF"
                     /locus_tag="O0V33_000915"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCX8975577.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTHGGKKMTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLAKIKSLVNIPIIASGGGGNAQHFVELFDQ
                     TDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR"
     gene            complement(2108..2812)
                     /locus_tag="O0V33_000916"
     CDS             complement(2108..2812)
                     /locus_tag="O0V33_000916"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCX8975578.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKAQLMDYFAAGINYC
                     IVGTKGIQDTEWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVKQLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLK"
     gene            complement(2805..3383)
                     /gene="hisH"
                     /locus_tag="O0V33_000917"
     CDS             complement(2805..3383)
                     /gene="hisH"
                     /locus_tag="O0V33_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCX8975579.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNKQNIIDQAETIILP
                     GVGHFKDAMSEIKRLNLDAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGTDIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            complement(3380..3958)
                     /gene="hisB"
                     /locus_tag="O0V33_000918"
     CDS             complement(3380..3958)
                     /gene="hisB"
                     /locus_tag="O0V33_000918"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HCX8975580.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLAR
                     VVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQCVPSSKGVIE"
     gene            complement(3927..4940)
                     /locus_tag="O0V33_000919"
     CDS             complement(3927..4940)
                     /locus_tag="O0V33_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HCX8975581.1"
                     /translation="MIYIDKNESPVTPLDEKTMTSIISTTPYNLYPDAAYEQFKDAYA
                     KFYGLSSEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDA
                     GSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKAINGYFVID
                     EAYLDYGTAYDVEMAPHILRMRTLSKAFGIAGLRLGVLMSTAETIQRIQTIEHPYPLN
                     VFTLNIATYIFRHREETRQFLTMQRQLAEQLKQIFDTHVADKMSVFPSHANFVLTKGS
                     AAQQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            complement(4956..6206)
                     /locus_tag="O0V33_000920"
     CDS             complement(4956..6206)
                     /locus_tag="O0V33_000920"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCX8975582.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEKSV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSKEVL
                     AACYITQVNQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDDTADLDAIVYDVFAQAEHDELARTYVISEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACYVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFDQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            complement(6199..6813)
                     /locus_tag="O0V33_000921"
     CDS             complement(6199..6813)
                     /locus_tag="O0V33_000921"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCX8975583.1"
                     /translation="MLRIAIAKGRLMDSLINYLDAIEFTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGIADIGIVGSDILDERHYNVNNLLDMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAEAYFKSKGIDVELIKLNGSVELACVVDMVDGIVDIVQTGTTLEA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            complement(6831..7649)
                     /locus_tag="O0V33_000922"
     CDS             complement(6831..7649)
                     /locus_tag="O0V33_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HCX8975584.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVVYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLERYIPNDHPALVELKIWERWLHT
                     QGYNDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            complement(7954..9078)
                     /locus_tag="O0V33_000923"
     CDS             complement(7954..9078)
                     /locus_tag="O0V33_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCX8975585.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDSKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLDGIKRDTKRWKNE
                     VEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRQFFKGNRDYEE"
     gene            complement(9196..9318)
                     /locus_tag="O0V33_000924"
                     /pseudo
     CDS             complement(9196..9318)
                     /locus_tag="O0V33_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            9491..10006
                     /locus_tag="O0V33_000925"
     CDS             9491..10006
                     /locus_tag="O0V33_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HCX8975586.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFETKSVTENKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGIIVTGTINREKYGINFNQALETGGVMLGKDVK
                     FEASAEFSISE"
     gene            complement(10149..11120)
                     /locus_tag="O0V33_000926"
     CDS             complement(10149..11120)
                     /locus_tag="O0V33_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HCX8975587.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKVNPETKEIKQPFVSHKANPAAIKIHKDGRLFVCY
                     LGDFKSTGGIFAATENGDNIQDIIADLSTTYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVAPDFKTVTPIIQNISVANGIALSTDEKVLWVTETTVNRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDRDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGH
                     MLRSTHPQFIPGTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ"
     gene            11364..12320
                     /locus_tag="O0V33_000927"
     CDS             11364..12320
                     /locus_tag="O0V33_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCX8975588.1"
                     /translation="MNYQVLLYYKYTTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEED
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDW
                     IKENKALFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGQY
                     WDGKMYVFDDRISVDINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENEAK
                     YLGACSYECAKHERNRYVQANNISDNEWQHRLTNFDDLHQHA"
     gene            12472..13110
                     /gene="pcp"
                     /locus_tag="O0V33_000928"
     CDS             12472..13110
                     /gene="pcp"
                     /locus_tag="O0V33_000928"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCX8975589.1"
                     /translation="MHILVTGFAPFDNQNINPSWEAVTQLEDIIGTHTIDKLKLPTSF
                     KKVDNIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTKSIIDQGLPGALSNSAGTYVCNHVLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQVVGKPDTPSMPLENIVAGLTAAIEAISNDEDLRIALGTTE"
     gene            complement(13230..13996)
                     /locus_tag="O0V33_000929"
                     /pseudo
     CDS             complement(13230..13996)
                     /locus_tag="O0V33_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501475.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13986..14324)
                     /locus_tag="O0V33_000930"
     CDS             complement(13986..14324)
                     /locus_tag="O0V33_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCX8975590.1"
                     /translation="MSVSNQIMKGLLDGAILGLIAQGETYGYEIMTKLKNQEFPEISE
                     GSIYPVLMRLNKKGYVDTTIKKSSNGGPRRKYYTITESGLKELKVFKSKWQALNKGMI
                     NLFGGENDVK"
     gene            14491..14955
                     /gene="bstA"
                     /locus_tag="O0V33_000931"
     CDS             14491..14955
                     /gene="bstA"
                     /locus_tag="O0V33_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HCX8975591.1"
                     /translation="MTTKTVFDVIDMGLGYLVKVYDAWKVEKVLDDYHKPFSNTIHWQ
                     FGHVLTIFESALAVAGKENIDLNIYRPLFGNGSSPDEWKDEVPNIETILEGLQTLPER
                     ARNLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK"
     gene            15194..16612
                     /locus_tag="O0V33_000932"
     CDS             15194..16612
                     /locus_tag="O0V33_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCX8975592.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPIKPEAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDESSRKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLISLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVIALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
     gene            complement(17037..17945)
                     /gene="rarD"
                     /locus_tag="O0V33_000933"
     CDS             complement(17037..17945)
                     /gene="rarD"
                     /locus_tag="O0V33_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCX8975593.1"
                     /translation="MNTEYKKGIFLALSAYILWGILPIYWQFVDAIGAFEILAFRIIF
                     SVIFMIFILAVGQKQRHAFQRDMNQLLAKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFSLDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKNL"
     gene            18167..18742
                     /locus_tag="O0V33_000934"
     CDS             18167..18742
                     /locus_tag="O0V33_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCX8975594.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEDVSQYTKSSLMEMHGVAPKAI
                     SILEQALFQHQLHFKTEVHSSLPFLLTGDVPCNHAPKRQQMIDFIVATAALDIELLRS
                     LVTTEFIWSVPGRFDIYGPQILIQELSNHYKEIASLNIQSIITHGCLGSMHGIEILKT
                     GKEIHFAHFFEFENHKKDAKLSKVTSYIVVG"
     gene            18906..19922
                     /locus_tag="O0V33_000935"
     CDS             18906..19922
                     /locus_tag="O0V33_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HCX8975595.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILTSLINLFAKLRREHIEEAEV
                     DALLESRGFVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVIAALV
                     IGMIELLQILADKLDLHGAFWAFISSIEFDYLGYILVALFLITWLISSLIWKFGRIGH
                     KWSK"
     gene            20162..20962
                     /locus_tag="O0V33_000936"
     CDS             20162..20962
                     /locus_tag="O0V33_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HCX8975596.1"
                     /translation="MNIAKLEDYLQIEPTRYDQPSIEALNYYATRFMLTVPFENIDVQ
                     NGKPISVDIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPELMSATIHTPGGGRS
                     QNGSHASLVVSINNVFYVTDVGFGDLPLNAIPITLPDDTQPITDISGTFRAIFNSEDK
                     DIFYVQKYENNHWHTKYEAEFKAREIEEFDQNIEYNQTHPDSVFVKHLLITMPQSFGR
                     ATMSENHLTLTKDGTPEKLAITKDNYKQFLTTYFGLNVTISRLENSEN"
     gene            complement(21250..21762)
                     /locus_tag="O0V33_000937"
     CDS             complement(21250..21762)
                     /locus_tag="O0V33_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HCX8975597.1"
                     /translation="MEKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIVYRRKAKALVGGNQNWILFMGIVDILFGLIVIFNVGASSAFFIYMFAFW
                     FIFSSIAGLFTFSGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            22052..22811
                     /gene="vraD"
                     /locus_tag="O0V33_000938"
                     /pseudo
     CDS             22052..22811
                     /gene="vraD"
                     /locus_tag="O0V33_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
     gene            22801..24681
                     /gene="vraE"
                     /locus_tag="O0V33_000939"
     CDS             22801..24681
                     /gene="vraE"
                     /locus_tag="O0V33_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HCX8975598.1"
                     /translation="MTFNHIVFKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTILML
                     IIAYILILFQSSLFLKRRSILSMMKDSIKTDVTTAKVTTAEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNDFNVVASQ
                     DAQQFESKLSQQHITFSKNAYETITVDNVKDQVITLENGSDSGRTNSILSANTKLTGN
                     NAIITNTKSLPNIINIHLNKDLVVKGTKNETFRVTQEDKGKVYPLNLSFNSPVVEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKEKAQAIAKQFGDKIITYDDMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIIVIIVYTLIYIVFALIAFVHSNKLIKK
                     TI"
     gene            24774..24965
                     /gene="vraH"
                     /locus_tag="O0V33_000940"
     CDS             24774..24965
                     /gene="vraH"
                     /locus_tag="O0V33_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HCX8975599.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALAMIVIVLSTVTTPIIGI
                     PVGLLGGAYYLKRREEKGK"
     gene            25028..25984
                     /locus_tag="O0V33_000941"
                     /pseudo
     CDS             25028..25984
                     /locus_tag="O0V33_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(26052..>26386)
                     /locus_tag="O0V33_000942"
     CDS             complement(26052..>26386)
                     /locus_tag="O0V33_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCX8975600.1"
                     /translation="KCTFLSEYLYPVKFREKTRIGRFFNITNWYEEIHSTDEKYVIAT
                     IKNIKNKDIIDYSLYIKKGLVEPFIVFYNQENIFYISNAVIDIISVSKDTLGYIKSEF
                     NDHCASPY"
BASE COUNT         8518 a         4711 c         4117 g         9040 t
ORIGIN      
        1 ggggatttat gagctatcac agttataatg tttgctggtg tcgtatttta aacctaaaaa
       61 tatcatttct tctacaatga ttgtatcagg ataaaccatt acgcttgttc ctacattaaa
      121 tatatacagt taattttctt ccctcaaagt aagtatccta ctattttaaa tttattgttt
      181 aattactctt gaactagctt taacttaatc tcgaatttat gatgattagc ataaaaatac
      241 accccataaa gttagttttt tagtccaact ttcgggatgc acatcttagt tctaatggag
      301 attgtctttt aaatagacta tgacatttct atttcaggct agatatattt ttattcacag
      361 tataaataca aagaatataa agacaactat gatattaata ggtagtaacg acaaacataa
      421 ctttttagct ttttcgccat ttttcccttc ataacttcat tatatcgatt catatacttt
      481 tcaacatctt taacagtgac atctattcct gagttgctca attcactttt caaattatct
      541 ttggtttcgt gtcgtgatta tccactagtt atctataacg agttcacaac tttagtacca
      601 tcagagcatt catagttaaa atatgttgat tcgtatttta aacttgaaaa tgtcacgttt
      661 tccacaatat ttgtaagtgt atatgccatg atccttttcc ttatatctca gtccatactt
      721 gctttaccac tgttcgatat tttgtcgttc gcctttaaag ttattgcgct tatgatgtct
      781 acgcgctaat tctgcctcaa tttctgaaaa atcgacgcct agcgcatgca tcaagacaaa
      841 taaatgataa agttcatctg ctacttcaca tacaaatgct tttttgtcac ctttaattgc
      901 ttcaattacg acttcaaaag cttcttcacc atattttttt gtaatctttt ctataccttc
      961 tgttaataaa tattttgtat atgacttttc attattggat tgggcactat cttgaactgt
     1021 ctgcgctaat gtttgcactg aaaatggaac ttctgtgttg aaacaacttt gactgcctgt
     1081 atggcatgtt ggtccattgg gtatgacatc aattaaaata gtgtcattgt cgcaatctac
     1141 atgaatatct ttaacacgtt gcgtatgacc agatgtttca ccttttgtcc atagccgttg
     1201 tttcgaccgc gaatagaagc ataccacacc atcttctatc gttttatcaa aagcttcttg
     1261 attcatataa cctaacatca atacttgttt tgtttgatta tcttgtaaaa ttgctggcac
     1321 taaacctttg ctaaaatcaa ttttgtaatt ggtcatctta ctgctatacc cccttgccgt
     1381 atcacttctt taatagattg aaccgtcgtt tctcgatcat gtaatatact tgcagctaaa
     1441 cctgcagaaa catccgtctg atcaaataat tctacaaagt gttgtgcatt gccaccacca
     1501 ccagaagcaa tgattggaat atttacaagg gattttattt ttgcaagatg ctcaatatca
     1561 aatccttgtt tcataccatc atgccccata cttgtaacga ggagctcacc tgcacctaac
     1621 tgttctactt gctgtaccca gtcatatact ttaatatttg tcatcttttt accaccatgc
     1681 gtacaacaat aatgtgcttt tctttcagga tcataatagc tatcaattgc tatgcagatg
     1741 cattgtctac cgaatttatc actcgcttgt ttaattagct gtggattttt taaagcactt
     1801 gaatttaatg atactttatc tgcaccatga tttaacaatt gggtaatatc atcaaggctt
     1861 tgaatcccac cccctacagt aagagggata aacaagcgtg acgctgtctg ttcaatcact
     1921 tctagcatta agctatgacc ctcttctgtc ttagagatgt ctaaaaatac taactcatca
     1981 gcacccgctt cattgtaata cattgctaaa tcaacaggat tcccaatatc ccttaatcct
     2041 ttaaattgaa tccctttaac gacacgacca tctttgacat ctaaacatgg aatgatacgt
     2101 tttttaatca ttttagcccc tcccaaaaag atgcttgatg tgcagccttt cctataatag
     2161 cagcgtgaac atttagcgac gctaatcgtt gaatatcttg ctgatgtcta ataccaccag
     2221 acgcgatgac aggaatcgtc gttgccttta ctaattgacc agttaattca aagttaggtc
     2281 cggacatttt gccatcttta gcgatatcag tatatataat gcctccaaga ggtatatccg
     2341 ataactgttt cacaaaacta aataaattca actctgtgtc ctcttcccac ccgttcactt
     2401 taatatcttc tccataagca tcaacagata aataaatgcg acctggaaat gtatgtgcca
     2461 tctctttaag ccattctgta tcttgaatac ctttcgttcc aactatgcaa taattaatcc
     2521 ccgcggcaaa gtagtccatg agttgtgctt tcgtacgaat gccaccacct acttcaatat
     2581 ctttggttgt taatctcctc aatgacttaa tataatcaaa ctctcgggca tgctgtgcct
     2641 tagcacctat caagtcgacg atatgaatac gattcacaca ttcaaattga ctatagtaag
     2701 caatactttc ttcagccgaa cgcgacattt tttcttcact atcatattta ccctctgtta
     2761 acctcacact tgttgaccca atcaaatcaa tcgctggcca taattcaatc atttataaat
     2821 cccccttgta ttgcctgacg caaaatttgt aacccatatg taccgctttt ttcaggatgg
     2881 aattgaatac caatataatt gttaaattgg acaattgctg gaatatcagt accatactgc
     2941 gcatatgcaa tgacattttc tgacatcggc gcttggtaag aatgtacgaa ataaacatct
     3001 tgatttaaca tagggtgctt actcactaaa ttattccagc ctaagtgcgg cactgggtat
     3061 tctgtttgga tacgcgaaat attccctggg ataaacccta atccagatgc atcgccttca
     3121 tcactatgct catacattaa ttgcatgcct aaacaaatac cgatcatttt cttatcagta
     3181 ttcttagcca atattgcatc gagatttaat cgctttatct ctgacatcgc atctttaaaa
     3241 tgaccgacac ccggtaatat gattgtttct gcttgatcga ttatattttg cttatttgag
     3301 acaaccacct cataccccaa atgttcaata gcgcgtttta cattactaat attccctaat
     3361 ccataatcaa cgatgacaat cattcaatca cacctttcga tgacggcaca cactgatcat
     3421 cagttgcagt tagcgctatg cctaatgcac gggaaaatgc tttgaatatg gcttcaattt
     3481 catgatgtgt attacctcca cgaattaaat caatatgcgt tgttagtctt gcattgatta
     3541 cgaccgctct aaaaaactct tctactaact ccgtatcaaa cgtgcctact ttttctttac
     3601 ttaatgatgc attgaatgat aggtatgggc gcccacttat atccacaacg acacgtgcta
     3661 atgtttcatc cattggaata tacattgttc cataacgaac gaaatgcttt ttatctttaa
     3721 tcatttcaag taacaattgg ccaataacaa tgccgatatc ttcagttaca tggtgatcat
     3781 ctacatcgat atcaccttgt gcctcgatgt ttaacgacag tccactatga aatgtaaaca
     3841 aggttaacat atgatttaaa aagcccacac ctgtattaat atgcgatggt gactggtcat
     3901 ctgatattga aatatttagt tgtgtttcag ctgtgtttcg ttgtttttga taaatcatat
     3961 tttgcactcc attctttaac aatttcttct aattgcttta actgtgatgc tgttgcaatt
     4021 gaatatctta catagccctt catcaccggc tcatcataaa aacgaggttt aaatccttgt
     4081 tcatatacgt attgtcctaa ttgttgcgcc gctgaacctt tagtaagtac aaaattggca
     4141 tgtgatggga atactgacat tttatctgca acatgtgtat caaatatttg ttttaactgc
     4201 tcagctaact gtcgttgcat tgttaaaaat tgtcttgtct cttctctatg tctaaaaata
     4261 taagtcgcaa tatttagcgt aaatacattt aatggatatg gatgttctat tgtttgaata
     4321 cgctgtatcg tttcagcagt actcattaat acacccaacc ttaagccggc aattccaaac
     4381 gcctttgata atgtgcgcat tcttaaaatg tgtggtgcca tttcaacgtc atatgccgta
     4441 ccataatcta aatatgcttc atcaatgaca aagtatccgt ttattgcttt catcttatct
     4501 gcaatagctg ttaaaaatgc cgtatcaaat tgcttgcctg aagggttatg tggattactc
     4561 ataataaaaa atgatggttg tacttcatcg attttcgtta aaatggtttc caaatcaaac
     4621 gttaaatctg atcctgcatc tacaaatgca atttcacgat ttacttgtgc cgcatatgct
     4681 tgatacataa aaaaatctgg atttagcgtt aatgccggac cttctggcat gatcagcatt
     4741 aacttttgaa tcaattcatc agagccattt cctgcaataa tttgttcaga cgataatccg
     4801 taaaacttag cataagcgtc cttgaattgt tcatatgctg catcaggata taaattatat
     4861 ggcgttgtac taataataga cgtcattgtt ttttcatcca acggcgtaac tgggctttca
     4921 tttttatcaa tataaatcat ttcatttaca ctcccctaag actgacgtat taaaatagac
     4981 tgttggtgat tgtataatgc ttcaacatgg gcaatgtgtt gtgctgaatc agcaatttga
     5041 tcaaatgtat cttttgataa atgaatgacc gtgttacgtg ttaagaaatc attgaccgat
     5101 aacccattag taaatctagc tgttctattt gtaggtaata catgactagg tcctgcaacg
     5161 taatctccta tgacctctgg cgaataatgt ccaataaaca atgcgcccac atatttcact
     5221 ttttcaatat atggttgagg atttactgtt tgaatcgacg catgttcagg tgcgattgta
     5281 ttcatgacat agcatgcttc atcaaaatta ctagcatgga taaggtagtg ttgattagcg
     5341 atacttttag aaacaatgtc gtatctgtcc acattaggca atgctttagc aatacgtgat
     5401 tctaaatctt taaggacttg cgcatcttca ctaatgacat atgtacgtgc taattcatca
     5461 tgttctgctt gcgcaaatac atcatatacg atggcatcta aatctgcggt gtcatcaata
     5521 atcagtgcta tttctgttgg tcctgcaatt tgatcaatac ctacctgtcc aaataaatat
     5581 tttttggcat atgcaacaaa ttggtttcct ggacctacaa tcttatcaac tttaggtatt
     5641 gtttctgttc cataagtcaa tgcagcaata ctttgagcgc caccaacttg aaacacttga
     5701 ttaacttgcg taatataaca tgcagctaat acctctttgg atactccgtt aggttgaggt
     5761 ggtgtcacaa caacaatatt ttctacaccc gctacttgtg ctaaagtcgc tgtcattaga
     5821 accgttgatg gataacttgc tttgccacca ggcacataaa tacccacact ttctagtgga
     5881 tggtatattt cataacactc cactgatttt tctaactgtt gattcgtctg cttaatactt
     5941 tcttgatatg ctttaattct ttcataactt tgttgtaatg cttgttttgt tttttcatct
     6001 aatgtgtcaa atgctgcttt aatttgttca tgactaattt ctaaatgatc tgtcttcgta
     6061 tgatcgaatg ttagattata cattttcaaa gccttatctc catgaacttt aacttcctga
     6121 caaatatcgc gaataattgg atacaatgac tcatctaatg gtgcttctaa tgaaaattga
     6181 tttaaaaatt gttgtgcatt aagcattggc tatagacacc tccaaagagc gaataaattg
     6241 ctcaattaat tgtgattttt taaaataagc agctttatta gtgattaatc ttgcattgat
     6301 atcactaata tgttgctttt caaccagtcc gttcgcttct agagtagtac ctgtttgaac
     6361 gatgtcgaca attccgtcta ccatatcgac gacacaagct aattcaacag aaccattcaa
     6421 tttaatcaat tcgacatcaa tgccttttga tttaaaatat gcttcggcag tatggacata
     6481 actcgttgcg attttacgat aattggtcgt ttcaggtttc gctgcaaccg caaaatgaca
     6541 tgctccaaaa ggcatatcca acaaattatt aacattgtaa tgacgctcat ctaaaatgtc
     6601 gctaccaacg ataccaatat ctgcaattcc ttgttccaca tagattggca cgtcactccc
     6661 ttttactaaa atgcattcta tattatctac acttaataat aattggcgtt ctctattttt
     6721 taatgtttct gataatgtcg taaattcaat tgcatctaaa tagttaatta aactatccat
     6781 tagacgccct tttgctatgg cgattcttaa cattgtgcat actcccttac ttaaagtgtt
     6841 aatcctaatc caaacccttc gatgcttcct ttgtaataac cacctgttaa aactcgagat
     6901 tcattttcag caaaatgaca ttgaataaat aaacctgtgt aataggatct aggtggctgc
     6961 gcagtaatat ctaaatggat gtcattgtaa ccttgtgtat gtaaccaacg ttcccatatt
     7021 tttaattcaa cgagtgctgg atggtcattt ggaatatagc gctccaatac atttaattgt
     7081 tgttgcgtat tctctttcaa gatttgcacg attggatgtt cagtagacaa atatgtaacc
     7141 aatcccgata aattacgttc ctcaatcatt gatagtatgt ctggtgtttg taacgattta
     7201 tcgagtaagg cgtcaagcaa ttgataatgt cccaatacaa caaaatgcac gttgtctctc
     7261 aactgctgtt gaataaactg aataaatagc tttaagctct gttgtacatt cgctaatgat
     7321 ggtgcatagt tttccaaacc aacttgtaca gccgcttcat tattccgaat gattaaacct
     7381 gtgtatacca cttttgttgc tgcagttgga tacattgagt aataacgcaa taattgatct
     7441 gtaaaatcat ttcgaagtgc ataaatttga tgctcatgtt gccaaaaatt tcgctcaccc
     7501 atctgctgca aatcctcatg gttcaattgt ttccagtcca acttttcaac cacactaaaa
     7561 tcaactaact cataatccgc tttattaaaa tattttaaaa atgctgtttc cgattctttt
     7621 aacgcaatta attgttctga attattcact cgaccaccct ttactttcaa tactgtattt
     7681 aaaatcactt ggtatttttg tcgttcgctt tacttctcta ccatactaaa gtgtaatatg
     7741 cttaataact tatcattttt agcaatacat tacaaccttt tttcagaaaa ttcggtgtat
     7801 tggttttaaa ttttttcaaa agaaaaaggc aagacatttg tgcctataaa aatgcttaac
     7861 caagattttt atattgaagt tgtacttctt gcacatattg tccttgcctt attatgtaaa
     7921 gttattttct ttctatcttt ttattaaatt taactattct tcataatccc gattaccttt
     7981 aaagaattgt ctatcttgtt tactatatac attttcagga ttaaaaaatg gtttgagttt
     8041 atatttaaat tcaaataagt ttagaccatc aatcgcaacc ctatcttggt aaacgttcgt
     8101 tgctgatata tctgtaaaat tgtttggacc gacacctgca ataaacttaa actctgcttc
     8161 atctaccaaa taatcatacg cttgtgtatg tctatcctgt gcgccatgtg gaaatacaaa
     8221 catatctgtt ttacctacaa ttggttcaac ttcatttttc catcttttag tatcacgttt
     8281 aataccatct aaagatgttt tttcaaaatt aatgtgacca tatgaatgac tcgcaaatga
     8341 ccatccatcc cgtttcattg cgcgaacaac ttcctcagct gcctttttat tctttgtata
     8401 atctttactc gttaattcat tcgtgcgata acctaatacg ccctcataac cggttaaagc
     8461 aacaacacct ttttcaccat ttaaagaaaa atctggatgc tcttttacaa atttatttaa
     8521 aattggcacg atatcattgt catcagaata agtagcatgg ccttttgagt ctgtcgtttc
     8581 agatacaaca tgcttatttt tatcaagtac taagcggtca gcataaccat ggtgtctcat
     8641 gtaactatag taattcatat catcaattga gatgattagt ggctttttac ctttcggcaa
     8701 ttttattttt ttggctttta catgatgaga tgataagtcg tatacatcat gtggattaac
     8761 gatgatgtaa ttatttttat ataattcgtt caatgatttt ttaaattcac ttacagtaat
     8821 catccaatca ttgttgccct tagcttggtg tgtatctcct gtaaacgcaa cttttgggtc
     8881 tgtaattaat ggatgataaa acacatgata aacttggccg tgatatgttt cccaatgttc
     8941 atccattttc gatttatgct ttgcatactc atttggatta acagatttat tttgagcttt
     9001 ctcattttgc ttggaacagc tatataacaa tgcaactgat aataacagaa aaaatagcaa
     9061 taaatatttt ttatgcatta aacattctcc ctatgtttta ttatgcataa ttataacatt
     9121 tttattatat ggctcataac tttttaagaa actgttggga ttttaatttt attttttaga
     9181 taatgtcata gtattttatt tataaaacac aatatctcct tgactgataa tgtcgtccgt
     9241 tgtatcgtct cgcttgaaat taagcctttc tgctaaattt attgaacgtt ggttacactt
     9301 ctttatgttt gaattaattg actagggtat aacaatagtg tggtgtttta taggtcattc
     9361 tttaaataca tcacagaaaa atattcgaat tcgaaataaa atgcttgaat ctttattttg
     9421 taggtggtat tgtatgtata tcaagtataa aaaatatatc taagagataa aaatatggag
     9481 gttttatatt atgactaact ttacttttga tggtgcacac agtagtttag aattccaaat
     9541 taaacattta atggtttcta aagtgaaagg ttcatttgat caatttgatg tagctgttga
     9601 aggagatatt aatgacttca gtactttgaa agctactgca acaattattc caagctcaat
     9661 taacactaaa aacgaagcac gtgataacca cttaaaatct ggtgatttct ttggtactga
     9721 tgaatttgat aaaatcacat ttgaaacaaa atcagttact gaaaacaaag ttgttggtga
     9781 tttaacaatt aaaggtatca ctaacgaaga aacattcgac gttgaattca acggagtaag
     9841 taagaatcct atggatggtt ctcaagtaac aggtattatt gttactggta caatcaatag
     9901 agaaaaatat ggcattaact ttaaccaagc acttgaaact ggtggagtaa tgctaggtaa
     9961 agatgtgaaa ttcgaagcat cagctgaatt ttcaatctca gaataatctc attacacaat
    10021 cctcattgtt ttaatatata cgaaatgcca actatatcat ccctaggtat agttggcatt
    10081 ttttaacttc taattaaaaa tcgctaagat ttaaacttat attaagacgc ctttcattca
    10141 ggtaggtctt attgaaactg aaaactttga tgaccttttg caaagccatt aaccgtataa
    10201 agcatagatc ctccgcccat ttctatatca ttggaacaaa tgatgagttg atttgtgcca
    10261 ggtataaatt gcggatgcgt agaacgtaac atatgccctt catctcggcc tggtatcaat
    10321 atttgtccta ttggataacc ccttttatta aatactaata ctcggccttg accatacatt
    10381 gccacatata aattatcatc tctatcaata caacatgagt ctggtccttc atgacctgta
    10441 aaatagtacg gtatggtagc tccaaatggt tgtatcgtca caccatcatc ttcaagtgca
    10501 atgcgatgta atcgattgac tgttgtttct gttacccata gtactttttc atctgtactt
    10561 aaagcaatac catttgctac gctaatattt tgaataatcg gcgtcactgt tttaaagtcc
    10621 ggcgcaacat aataaacgcc tcctagtggg ttggtagagt atcctctaaa atctgtaaaa
    10681 taaaatccac ctttagaatc aaataccatg tcatcaatac aataagttgt tgaaagatct
    10741 gcaataatat cttgtatgtt gtcaccattt tctgtagctg caaaaatgcc tcctgtagat
    10801 ttaaaatctc ctaaataaca aacgaataat cggccatctt tatgtatttt gattgctgca
    10861 ggattcgctt tatgacttac aaaaggttgt ttaatttcct tcgtttcagg attgactttg
    10921 aaaatattac cttcgaatac atccaataaa aatagttgtc cttgccgatc aaagttcaat
    10981 ccttctaatt gcaatccttt tttggaaatt tcaagccatg gttcagctgt aattgtttgt
    11041 aattcacttt cagatataat tggaacagca ctattagact tcccgctata aaataatgta
    11101 ggtaaatctt gttgtgacat catgatcatt cctttcattt cattcgtttt aactatcatt
    11161 tttattttaa acaagggaac atttaaatca aatgataacg ttttcattta cctgttttat
    11221 cggtctagtg gctgatttca agctagaaat attgaatgac aatacaactc tgttaaaatg
    11281 atggacgtag acaaatatgc gtattgacgc tttattttaa aaattaacat gcttataaca
    11341 tgtttttaga aggagattaa cctatgaact atcaagttct tttatattat aaatatacga
    11401 cgattgatga ccctgaacag tttgctcagg atcacttagc cttttgtaaa gcacaccatt
    11461 taaaaggtag aattcttgtt tctacagaag gtattaacgg cacattatct ggtacaaaag
    11521 aagaaaccga acaatatatg gcacatatgc atgccgatga acgattcaaa gatatggtgt
    11581 ttaaaattga tgaagctgaa ggacatgctt ttaagaaaat gcatgtacgt cctcgaaaag
    11641 agattgttgc tttagattta gaagaagacg tcgatccaag acacacaact ggccaatatt
    11701 tatcacctgt agaatttaga aaagctcttg aagatgatga cacagtcatt attgatgcac
    11761 gtaatgatta tgaatttgat ttaggtcatt tccgaggtgc aattcgtcca aatatcacgc
    11821 gctttagaga tttgcctgac tggattaaag agaataaagc gttatttgca gataaaaaag
    11881 tggttacgta ctgtacaggt ggcattcgat gtgaaaaatt ttctggatgg cttttaaaag
    11941 aaggtttcga agatgtagcg cagcttcacg gcggtatagc tacatatggt aaagaccctg
    12001 aaacaaaagg tcaatattgg gacggtaaaa tgtatgtatt tgatgatcgt atcagtgttg
    12061 atatcaacca agttgaaaaa acgattattg gtaaggattg gtttgatggc aaaccatgtg
    12121 aacgttatat taattgcgct aacccagaat gtaataaaca aatattagtt tctgaagaaa
    12181 acgaagctaa atatttaggt gcatgctctt atgaatgtgc taaacatgag cgtaatcgtt
    12241 atgttcaagc aaacaatatt agtgataacg agtggcaaca tcgtttaacc aactttgatg
    12301 atttacatca acatgcttag ttttaattaa atacctatca aaacacgctt tgaaaatccg
    12361 atttataaag gtttttcaag gctgtttttt atgcgcatat ataacctgat atgtttttaa
    12421 ttatgcgcat gttatactaa attaaatttt aatactgcgg ggtgtcttaa aatgcacatt
    12481 ttagtaacag ggtttgcgcc ttttgacaat caaaatatta atccttcttg ggaagctgtg
    12541 actcaactag aagatattat tggcacacat acaatcgata aattaaaact accaacctct
    12601 tttaagaaag tagataatat tataaataaa acgttggcat ctaatcatta tgatgttgta
    12661 ctagctatag gacaagctgg tggtagaaat gccattaccc cagaacgtgt cgccattaat
    12721 attgatgatg cacgtattcc agataatgat gattttcaac ctattgatca agccattcat
    12781 ttagacggtg cgccagctta tttttcaaat ttaccagtta aagcaatgac aaaaagtatt
    12841 attgatcaag gacttccagg agcactttca aatagtgcag gtacgtatgt atgtaatcac
    12901 gtgctttatc acttaggcta tttacaagat aagcactatc ctcatcttcg ctttggattt
    12961 atccatgtgc catacatacc agagcaagtc gttggtaaac ccgatacacc atctatgcca
    13021 ttagaaaata tagttgcagg tttgactgca gccattgaag ctatttctaa tgatgaggat
    13081 ttacgtatag ctctaggcac aacagaatag ttaaaagtac acaaacaaaa gcaccctaat
    13141 ttgttagttt aagtctaact ttagggtgct ttaagagggc ttatttgata tttctattgt
    13201 agagatacac tttagctaca tgacattgtt tagtcttctt tttttaactt cctataattt
    13261 acaaatgtat aaataataaa tgctatattt agaatgatga gtatacttac tgaaactaaa
    13321 ttaaaagtgt ctggttcttt actaaagata gatgctatcc ttgctatcaa acttggcaat
    13381 gaaattacca acacgatgac cgcataaatt tttgttttat taaaatacaa attacaataa
    13441 gcgcaattaa aagtactaac ccaacgatga aactctgttt gtcacttaac tcaaagaaac
    13501 tatagatagg atatttttta ataatcaagc caccaaaaat catccataaa aatacgacaa
    13561 ttccataagt cacatttata acatacgtta ttttttggtc accaaatcgg actaatgtat
    13621 tgcgtagaat cagcatacca atgacaacac ctaaaataac gatactagct atataaagta
    13681 aaaatgcaat tgtcacatca aatgtaccca aatctaaaaa cctaggaatt ataatgactg
    13741 ctaatataaa agagaagtac aaagtaatat atttatacaa accggtagta agacttatct
    13801 caggtgataa ctgatcagcc attgacttaa tcggtgtatt aataattgaa cttgtatctt
    13861 cgttattttt ttcagccata gttaaatgat cttcgagctc ttccaatagt tcttctactt
    13921 cttcttcagt tttacctcta aataacaatt caacacgtaa tttttctaaa aaatcttgag
    13981 actgtttact taacatcgtt ttcccctcca aacaagttaa tcattccttt attcaaagct
    14041 tgccattttg atttaaatac ttttaattcc tttaaacctg actcggtaat cgtatagtac
    14101 tttcgccttg ggccaccatt actagatttt tttatagtcg tatcaacgta tccttttttg
    14161 tttaaacgca ttaaaactgg ataaatacta ccttcactta tctctggaaa ctcttgattc
    14221 ttaagtttcg tcataatttc atatccatat gtttcgcctt gggcaatgag acctaatatt
    14281 gccccatcta agagaccttt cataatctga tttgacactg acattttcat cacctactat
    14341 cttacattat aagatagtac attgaggact tttcgtcaac tatcttatat tataagttag
    14401 tcatgattta cattccttaa atgactaaca aatttgttaa tagggtaata cttacggaag
    14461 tatgttttat ttatggggga ggaattaata atgactacaa aaacagtatt tgatgtcatt
    14521 gatatgggat taggatattt agtaaaagtg tatgatgctt ggaaagttga aaaggtactt
    14581 gatgattatc ataagccttt ttctaatacc attcattggc aatttgggca tgtattaaca
    14641 atttttgaat cagccttagc tgttgctggt aaagagaata ttgatttaaa tatatataga
    14701 cctttattcg gtaatggttc gtctccagat gaatggaagg atgaagtacc gaatattgaa
    14761 acgattttag aaggtctcca aacattacct gaacgtgcac gaaatctaac tgaagatgat
    14821 ttagcaattg aattgaaaca gccaattgtc ggttgtaata acttagaaga gttattagta
    14881 ttaaatgcca ttcacatccc acttcatgct ggtaaaattg aagaaatgtc tcgtatatta
    14941 aaaaatttaa aataaatatg tgcttattaa ccgttaacaa cacgttaacg gattttttta
    15001 tttgtttaaa aggccactct tttaaattta ataaacacca actataccag ttcttcactg
    15061 cttctcgaaa aataattata ttaatgattt cgttaattga attttatatt taattattac
    15121 tgtacatctt ttgtagttag ctttattctt aaattgaaat atgtataaaa catagaaaga
    15181 aggacgtttt aacatggaaa acacggttaa atatcgtaag tttatactcc ctatcgttgt
    15241 aggtctcctt atttgggcac ttacacctat taaaccggaa gctgtggatc caacagcatg
    15301 gtatatgttc gcaatattcg tcgcgacaat cattgcttgt attacacaac cgatgccaat
    15361 cggggctgtc tctataattg gatttacaat catggtgctc gttggcattg ttgacatgaa
    15421 aacggctgtc gctggttttg gtaataatag tatatggtta attgctatgg catttttcat
    15481 ttcgagagga tttgtgaaaa caggtcttgg tagacgtatc gcacttcatt tcgtcaaatt
    15541 atttggtaaa aaaacattag gcttagcata ttctatcgtc ggtgtagatt taattctagc
    15601 gcctgctaca ccaagtaata ccgcgcgtgc tggtggaatc atgttcccaa ttatcaaatc
    15661 actttctgaa tcatttggtt cgaaaccgaa agacgaatca tcacgtaaaa tgggtgcatt
    15721 tcttgttttc acagaattcc aaggtaattt aattactgcg gctatgtttt taactgcaat
    15781 ggccggtaac ccccttgcac aaaatttagc atctagcaca tctaatgttc acattacatg
    15841 gatgaattgg ttcctagctg ctttagttcc tggacttatt tccttaattg ttgtaccttt
    15901 tattatttat aaaatttatc caccaactgt taaagaaaca ccaaatgcta aaagttgggc
    15961 tgaaaatgaa ttagcgacta tgggtaaaat cgctttagct gaaaaattta tgattggtat
    16021 ttttgtcatt gctctaacac tatggattgt cggaagtttc attcatattg atgcaacttt
    16081 aacagccttt atcgcgctag cattgttatt attgacaggc gtcttaacat ggcaagacat
    16141 tttaaacgaa acaggtgctt ggaacacatt agtatggttc tcagtgttag tgttaatggc
    16201 cgaccaatta aacaagcttg gatttattcc ttggttaagt aaatccattg ctacaagtct
    16261 tggtggctta agctggccta tagtactggt cattttaata ttgttctact tctattcaca
    16321 ttacttattt gcaagttcta cagcacatat cagtgcgatg tatgcagcat tactaggcgt
    16381 tgccatcgca gccggtgcac caccattatt cagtgcatta atgttaggtt tcttcggtaa
    16441 ccttttagct tcaacaacac actatagtag tggtccagcg ccgattctat tctcttcagg
    16501 ttacgtgact caaaaacgtt ggtggacaat gaacttaata ttaggtttcg tctactttat
    16561 tatctggatt ggtttaggat cactttggat gaaagtaatt ggtatatttt aaaatattta
    16621 aattagcgct cgaacctcat tgatttgggc gctttttaat ttgtatttaa aatcaatcct
    16681 tgctaaatca agactccctt tttaaaatac gtttatcttt taaatcattg catacttcac
    16741 tgaaaatttg tataaagatt taagtcatta cgtaacatca cataaaatac atttctatac
    16801 taatccgctt cattgattaa cattacgtat tccctcataa atcataaaat aaaaaaacac
    16861 cttcgtttaa attcatttta agtgcgaatt caacgaaagt gatttatttc atatttaatg
    16921 ttttaaattt atacagctgt cactgttact gcacacatac ctcagtctta cctaaacatg
    16981 gcacaaatag aaaataatca catatacaat ttagcaattt tctcacttta atcattttat
    17041 aggttttttg cgactggatg tttcttcaat ttaatgtatt gagaaagact atataacaca
    17101 atacctgtcc aaataaatat aaacgtaatt aattgatcta aactaaaagg ctctttgaaa
    17161 acaaatatgc cgagtacaaa cattattgtt ggtccaacat attgaataaa tcctattagc
    17221 gaaagtggaa tacgttttgc cccggctgag aataggatta gtggtattgc cgtaatagca
    17281 ccagaaaata acaaccaaaa tgatgacatg ttcaatccaa atgacatctg atgttgctgc
    17341 cataaataaa taacgtatat tagtccagca ggtgcggtaa caatacattc aatcgtaata
    17401 ctgctgatgg catcaatatg tactactttt ttcaataatc catatgtacc aaaggataac
    17461 gctaatataa tagagacgat tgggaattct ccaattttca gtgtcatata tagtacacca
    17521 atgaatgcga ataatatggc taaccattca aatttattga atctttcttt taaaaagata
    17581 agtgcgagta gaatactgac aagtggattt atataataac ctaaacttgt ttgtaggacg
    17641 tgtccgttcg ttacagccca aataaatgta ccccaattca aggtgatcac ataacccgct
    17701 acgacaattg ctagtaattg aatgggcttg gctaacaatt gattcatatc tcgttgaaat
    17761 gcatggcgtt gtttttgtcc aaccgcgagt atgaaaatca tgaatattac tgaaaatata
    17821 atacgaaagg ctaaaatttc aaatgcgcct attgcatcaa cgaactgcca atatataggt
    17881 agtatgcccc aaagaatgta agcactaagt gctaaaaata tacctttttt atattctgta
    17941 ttcacttaca tacctcctta ctctcccaaa tttttaacat gttacgctca cttagttaag
    18001 ctaatataac gattttacta ataatatttc gataaagata tcattttgtt tatattttcc
    18061 acatttattc gccaaccact aaacaatatt aattttataa ataattctgt acaaatcagg
    18121 gtatattgcc agaaagacta ccaaacaact taaaggatgg atacaaatga ctttacctaa
    18181 aattggaaag cctgcaacac gtgcattaaa ttcacaaggt atatacacat tagaagatgt
    18241 atcacaatat acgaagtcat ctctaatgga gatgcatggc gttgccccta aagctatatc
    18301 aatattggaa caagctttat ttcagcacca actacatttt aaaacggaag ttcattcatc
    18361 attacctttt ttattaacag gagacgtgcc atgcaatcat gcccctaaac gtcaacaaat
    18421 gatagatttt atagttgcta cagcagcatt agatattgaa ttattacgtt cactggttac
    18481 aacagaattt atttggtctg tccctggccg ctttgacata tacggacctc aaatattaat
    18541 tcaagaacta tctaatcatt ataaagagat tgcttctctc aatattcaat ccatcatcac
    18601 acatgggtgc ttaggttcca tgcatggtat tgaaatatta aaaactggta aagaaattca
    18661 ttttgctcat ttttttgaat ttgaaaatca taaaaaggat gccaagttaa gcaaagtgac
    18721 atcttacatt gttgttggtt gatgtatcaa attttatatc gcaaaaaatt ctttttcggt
    18781 tacatatata tttttatata acaaataatt ttgcataaca tttttgttat atgaacactt
    18841 attactagag tgcatattga acatacagta tgattaaata tcatgttatg aaagaaggat
    18901 atattttgac tgtttttaaa aatgagcgct taagctggtt accatacata gctattgtca
    18961 ttttgttaca cgttattggg tttagttttt tatggattgc tggaaaagac catcatatct
    19021 tatttggtat ggggattctt gcatatacat taggtttacg tcatgcattt gatgctgatc
    19081 atatcgccgc gatagataat acggttcgca aattattaca gcaacgcaaa gatccatctg
    19141 gtgttggttt ctacttttca attggacatt catctgttgt atttttaatg gctgtttttt
    19201 taggggtatc tgtaaaatgg gctaaagatg aattaccaca tttccaagat attggtggaa
    19261 cgattggtac actagtttca ggtttctttt tagtacttat cggtgtgttg aatctaatta
    19321 ttttaacctc tttaattaac ttgttcgcta aattacgtcg tgaacacatt gaagaagctg
    19381 aagtcgatgc attacttgaa tctcgaggat ttgtttctcg atttgtaggt ccttatttca
    19441 aattaatcac acgtagttgg catgtattgc cacttggctt tttatttggg cttggttttg
    19501 atacagctag tgaaattgcg ctactcgctc tttcttcagg tgcatcacaa caagccattt
    19561 catttatcgg aatcttatct ttacccattt tattcgcatc aggtatgagt ttgttggata
    19621 ctttagatgg tgttgtaatg aaatatgcct ataattgggc attcttcaat cctattcgta
    19681 aaatctatta caatataacg attactgcga tatccgttat agctgcatta gtgattggga
    19741 tgattgaatt gctgcaaatt cttgctgata agctagactt acacggtgca ttttgggcat
    19801 ttattagttc aatcgaattt gattatttag gctatattct agtcgcttta tttttaataa
    19861 cttggcttat ttcaagttta atttggaagt ttggtcgcat aggacacaaa tggtctaaat
    19921 aaagtagctt gtcttgcaat catcttttta ttattgcgtt tttagacacc ctgcatactg
    19981 actcctttag gatacgctct ctattgtcat attttgaatc ttacttttaa agatgacagc
    20041 attgagagcg tatttttcta tctaaaatac atcttgtaaa atccagtgct ttcttattta
    20101 aaattttagc atttctgttt acaattctct tatagttttt aatacaggag gcggtatact
    20161 aatgaatatt gcaaagttag aagattatct acaaattgaa ccaactcgtt atgatcaacc
    20221 gagcattgaa gcattaaatt attatgcgac tcgctttatg ttaacggtgc ctttcgaaaa
    20281 tattgatgtt caaaatggta aaccaatatc tgtagatatc gatgcacttt tcaataaaat
    20341 tgttcatgac aaacgtggtg gcttctgtta tgaattaaac acatttttca aagcatattt
    20401 acaacaaaaa ggatttaacc cagaattaat gtcggctacc attcatacac ccggtggcgg
    20461 tcgtagtcaa aacggttcac atgcatcact tgttgtttct attaataatg tcttttatgt
    20521 aaccgatgtc ggctttggag acttacctct aaatgccata ccgattactt taccagatga
    20581 tacacagccg attactgata tcagtggtac attccgcgct attttcaaca gtgaggataa
    20641 ggacattttc tatgttcaaa aatatgaaaa taatcattgg catacaaaat acgaagctga
    20701 attcaaggct agagaaatag aagaattcga tcaaaacatt gaatataatc aaacacatcc
    20761 tgattcagta tttgtaaaac atttattaat tacaatgcca caatcatttg gacgcgctac
    20821 aatgtctgaa aaccatttaa ctttaacaaa agatggaacg ccagaaaagt tagctatcac
    20881 aaaagataac tataaacaat ttttaacaac atactttgga ctaaatgtaa caattagtcg
    20941 acttgaaaac agcgaaaatt aatcataaaa atacggacgt aacttacttt aatgtaggta
    21001 acgtcctttt ttaatacaca tgttcgtcag ggggtaggac aaaaatgata aagcgccact
    21061 aataattgat gatttagtgg ttcttacaca ttagccaaag ctcgtgtgta cttataaata
    21121 ggcattcatg cgtaaaatac taatttctaa agaaaaaata tttctttatc gacactctgc
    21181 gcaaacacaa caaaaaggat atggcacaaa cttcatgcca cacccttctt ttgaaccgct
    21241 tcattttact taagcaagtg catctacaac ataaatgaca cctacgaata caaatgcaat
    21301 agcaatcatc gtcgaaataa agacgatacc cattaatgga ttaaataata aaatgacacc
    21361 gaaaacaata cctaataaat taaaaatcac tgaaattagt tttaagctac cactacccga
    21421 aaacgtaaat aatccagcaa tagaactaaa aataaaccaa aaagcaaaca tgtagataaa
    21481 aaatgccgag ctagcaccaa cattaaaaat gacaataaga ccaaagagaa tatctacaat
    21541 ccccataaac agaatccaat tttggttacc gccaactaaa gctttggctt ttctacgata
    21601 aacaatttga atcacaccat taatcaatac aaataatcca attaaccatg tgattgcata
    21661 aaagttttca attgggaacg taaaaataac aactgctaac attaataaca gcacacccat
    21721 aatcaaacta gaccatttaa tgccttgatt tcctttttcc ataacaatca tttcctttca
    21781 aacaacgcct tctttatatt ttcattagta ccactattgt tcattgatta aattccctca
    21841 tcttaactta tagaaacatt tcctcagtgc aaatactgcg atgctcaaat tagttgaatt
    21901 gctttccaat aatccaaatt cacccgagaa aatcacgata tattaatatc tttcaactat
    21961 gtaagattca aacattgaat tgtaagttta gataaaggct actgttctac ttattattta
    22021 aaatgaaatt aaatacaaaa ggagtgatac tatgacgata ttatcagtgc aacatgtttc
    22081 aaaaacacac ggtaaaaagc acacatttca agcacttaaa gatattaact ttgacataca
    22141 aaaaggcgaa ttcgttgcga ttatggggcc ttctggatca ggtaagacaa ccttattaaa
    22201 tgtactaagt tcaattgacc aaatttctag aggtagcgtg attgctaacg ggtcaagagc
    22261 ttaataaact taatcaaaaa gcacttgcca aattccgtaa agaatcatta ggtttcatct
    22321 tccaagatta cagtattctg ccgacattaa ctgttaaaga aaacattatg ttacctttat
    22381 ctgttcaaaa aatgtcgaag gcaacaatgg aagaaaatta taaagcgatt acgacagcat
    22441 taggtattta tgacctggga aataaatacc ctagcgaatt atctggtggt caacaacaaa
    22501 gaactgcagc agcgagagca tttgttcata aacctcaaat catatttgca gatgagccaa
    22561 caggcgcact cgactcgaaa agtgcaaatg acttattaca acgtttggaa gaaatgaata
    22621 aatcgtttga taccactatt gtcatggtta cacatgatcc ggttgcagca agttttgcag
    22681 aacgtgtcat catgctaaaa gatggccaaa ttcatacaca actttatcag gaaggacgtt
    22741 ctaaacaggc cttttatgaa gacattgtac atcttcaatc agtattaggt ggtgtctcaa
    22801 atgacattta accatatcgt tttcaaaaac ttacgccaaa acttaaaaca ttatgcaatg
    22861 tatttatttt cattattttt tagtatcgtc ttatatttca gttttacaac cttacagttt
    22921 actaaaggtg taaataatga cgactcgatg gccatcatta agaaaggtgc tttagtcgga
    22981 tcaatatttt tatttatcat tattgtcatc tttttaatgt atgccaatca tttattcgtg
    23041 aaacgtcgta cacgtgaatt tgcgctattt cagttgattg gtttaacacg acaaaacatt
    23101 ttgaaaatgt tagcgcttga acaaatgatt gtatttttaa tcacaggtgt tgtcggtgta
    23161 ttatgtggca ttgcaggtgc acaattattg ctttccattg tatctaaatt gatgtcgtta
    23221 tcgattaact tatcgataca tttcgaacct atggccctcg ttttaactat tttaatgcta
    23281 attattgcgt atatactgat tttatttcaa agcagtttgt ttttaaaaag acgcagtatc
    23341 ttatcaatga tgaaagactc aattaaaact gatgttacaa ctgctaaagt aacgactgcc
    23401 gaggttattt caggcgtatt aggtattgct atgattgcac taggctatta tatggcgaca
    23461 gaaatgtttg gtacgttcaa agcactaact atggctatga catcaccgtt tatcatttta
    23521 tttttaacgg ttgtaggcgc ctatttattc ttcagaagtt ccgtgtcact gatttttaaa
    23581 actttgaaaa aatctaaaaa tggacgcgta tctattacag atgttgtatt tacatcttct
    23641 attatgtacc gaatgaagaa aaatgccatg tctttaacta tcattgcaat tatttctgca
    23701 gttacagtaa ctgtattgtg ttttgcggca ttatctaaat caaataccga tcaaaccctt
    23761 acatctatgg cacccaatga ttttaacgtg gtcgcctctc aagatgcgca acaatttgaa
    23821 tctaaactaa gccaacaaca tattacattt tctaaaaatg cttacgaaac tattactgta
    23881 gataatgtta aagatcaggt tattactttg gaaaatggta gtgattcagg tcgcacgaat
    23941 tctattttaa gtgcaaatac aaagttaact ggtaacaatg ccatcattac aaatacaaaa
    24001 tcacttccta acattattaa tatccattta aacaaagatt tagtagtaaa aggtactaag
    24061 aatgaaacct tccgtgttac acaagaagac aaaggtaagg tttatccctt aaatctaagc
    24121 ttcaactcac ctgtcgtcga agtaagtcct gaaaagtatc agcagttgaa aacacaaaat
    24181 aacgttcata ctttttatgg gtatgatatt aaacaaacat cacaaaagga aaaagctcaa
    24241 gcaattgcaa aacagtttgg agacaaaatc atcacttatg atgacatgaa aaaagaggtc
    24301 gatgctacta acggtattct aatatttgtt acatcatttt taggattagc atttttagta
    24361 gcagctggat gtattattta tattaaacaa atggatgaaa ctgaggatga actaagtaac
    24421 ttcagaatat taaaacgcat aggctttacg catacagata tgcttaaggg attattatta
    24481 aaaattactt tcaacttcgg tttaccatta ttgattgcta tattacatgc agtattcgcc
    24541 gccatagcat ttatgaaatt gatgggtaat atatccttca tgccggttat tatagtaatc
    24601 attgtataca cactcattta cattgttttt gctttaatcg cttttgtaca ttcaaacaaa
    24661 ttgattaaga aaaccattta acacttgtga ttaaaagatg cttcattgag attttgattt
    24721 taagcaatgc agtgtaacta ttaactgatt tataaaaaag gagagattga atcatgaaat
    24781 ttagagaatt agtaaaacaa tcatacgagg atttgaaaaa tttgactgta acttggtata
    24841 acgtgattgc tttagcaatg attgttattg tcttaagcac cgttacaaca ccaataatag
    24901 gtattccagt tggtttatta ggtggcgctt attatttaaa acgccgcgaa gaaaaaggca
    24961 aataagcctt ctgtgctaaa ctcaatatgt atttcctacc aaagaaaaca taaggagtta
    25021 gtacagaatg gcctatacac accttaacac ggatgaactt acgttcatag actcatatta
    25081 tcactagaat ctttcagtta aagagatttc gagtagattg aaacgttcta gacagacaat
    25141 ttataacgta atcaatgctt ttaagtcggg tatgtctgcc cttgaatatt atcaagaata
    25201 caaacaaaga aagtctaatt gtgggagaca tagaatagtg cttccagaag aacagtcaga
    25261 ttatatccga gagaacgtag ctgatggata gacacctgat actattattg gcagaggtga
    25321 acacacgatc aattgttctg ttaaaacact ttacagaatg tttaaagaga acatttttgc
    25381 tgtacatgat ttacctatga aatgtaaaag aaagccaaat ggccattctg aaaaaagagg
    25441 tagatagact tttaaaagac atatttctga aagaatagaa gagttccctt tattcaataa
    25501 ggagtttgga catctcgaag gagatactat tgttggtcgt catggtagtg caatcattac
    25561 tttggtagaa agagtatcca agatgattat caccttacga tccttaggtc gtaaggcaac
    25621 tgatatagaa aatgctctga actcaatgtt ttctgcttta ccttctcata tatttaaatc
    25681 aattactttt gattgtggta aggagttctc aaactggaaa acaatatgta actgtcacga
    25741 tatatctata ttttttgcta atccaagcat gccagcacag cgaggtttaa atgaacattc
    25801 taacggaata cttagacgca gtggattaga taaggaactg gatttcaatt tagtaacgga
    25861 tgatcatatc atcagtgttg ctcaaaaaat caatcatcgt cctagaaaat ctttaggtta
    25921 tagaacacca ttggaagtat ttatgagttt catcaaagat gataaactgt ccagcttaat
    25981 ttgacaatct aaagatttat aaaaaagccg ttggtctctt cgaaactacg gcttttctct
    26041 gtgctataaa atcagtatgg cgaagcacag tgatcattga attctgactt tatataacca
    26101 agagtatctt tggatacact gatgatatct ataacagcat tgctaatgta gaatatattt
    26161 tcttgattat agaatacaat aaatggctcc actaaacctt ttttaatata taaagaataa
    26221 tcaattatgt ctttgttttt tatattttta atagttgcaa taacatactt ctcatcggta
    26281 gaatggattt cttcgtacca gttagtaata ttaaaaaaac gaccaattct tgttttttct
    26341 ctaaatttaa caggatataa gtattcagac aaaaatgtac atttcg
//
LOCUS       DAKSQE010000026        25120 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.026, whole genome shotgun sequence.
ACCESSION   DAKSQE010000026 DAKSQE010000000
VERSION     DAKSQE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25120
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.026"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            77..1699
                     /locus_tag="O0V33_000943"
     CDS             77..1699
                     /locus_tag="O0V33_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCX8975601.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            complement(1869..2432)
                     /locus_tag="O0V33_000944"
     CDS             complement(1869..2432)
                     /locus_tag="O0V33_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HCX8975602.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINPLLESSDLTEAETQEAHKIKAQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQSSDSKN"
     gene            complement(2521..2616)
                     /locus_tag="O0V33_000945"
     CDS             complement(2521..2616)
                     /locus_tag="O0V33_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975603.1"
                     /translation="MKNVSIYKTTQELSFVSSLDFYLTKHLTLML"
     gene            2685..4175
                     /gene="betB"
                     /locus_tag="O0V33_000946"
     CDS             2685..4175
                     /gene="betB"
                     /locus_tag="O0V33_000946"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HCX8975604.1"
                     /translation="MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEIDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            4436..6145
                     /gene="betA"
                     /locus_tag="O0V33_000947"
     CDS             4436..6145
                     /gene="betA"
                     /locus_tag="O0V33_000947"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HCX8975605.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDANEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLHPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            6423..6635
                     /locus_tag="O0V33_000948"
     CDS             6423..6635
                     /locus_tag="O0V33_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCX8975606.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
     gene            6915..7358
                     /locus_tag="O0V33_000949"
     CDS             6915..7358
                     /locus_tag="O0V33_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCX8975607.1"
                     /translation="MNIYDEYRSYVIEELDDCLTIQKNNDTAYYDVLEAINDLSNDTL
                     CVLNHLYINEGQEETFEQKFLQRNKHLQNVDGFKALRFLRPRTAGRHYIIITLWENRQ
                     AFYHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADML"
     gene            7552..9150
                     /locus_tag="O0V33_000950"
     CDS             7552..9150
                     /locus_tag="O0V33_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCX8975608.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPDSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDAEIEKWQQQKDSNQ"
     gene            complement(9210..9541)
                     /locus_tag="O0V33_000951"
                     /pseudo
     CDS             complement(9210..9541)
                     /locus_tag="O0V33_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9837..11333
                     /gene="mqo"
                     /locus_tag="O0V33_000952"
     CDS             9837..11333
                     /gene="mqo"
                     /locus_tag="O0V33_000952"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCX8975609.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
     gene            complement(11526..12416)
                     /locus_tag="O0V33_000953"
     CDS             complement(11526..12416)
                     /locus_tag="O0V33_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HCX8975610.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVEGKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDSLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDSLNADQLVMLKLTIPTK
                     ANLYKDLAEHPNVVRIVVLSGGYSREKANELLKDNAELIASFSRALASDLRAGQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(12539..12955)
                     /locus_tag="O0V33_000954"
     CDS             complement(12539..12955)
                     /locus_tag="O0V33_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975611.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDIISNQSHLKALLLNLDF
                     LIPSDNTPYTLEIICHLLIGSVIYFVFVLLFHTSKRLYYLCYIPLVFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            13209..14546
                     /locus_tag="O0V33_000955"
     CDS             13209..14546
                     /locus_tag="O0V33_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HCX8975612.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCEIAPGDF
                     EKRVTFGLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALENICRENGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKNLKTRDASAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
     gene            complement(14626..16074)
                     /locus_tag="O0V33_000956"
     CDS             complement(14626..16074)
                     /locus_tag="O0V33_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCX8975613.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSIAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAVTFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
     gene            16760..17719
                     /locus_tag="O0V33_000957"
     CDS             16760..17719
                     /locus_tag="O0V33_000957"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCX8975614.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED"
     gene            17997..18701
                     /gene="budA"
                     /locus_tag="O0V33_000958"
     CDS             17997..18701
                     /gene="budA"
                     /locus_tag="O0V33_000958"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCX8975615.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFPLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQDIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(18787..19647)
                     /locus_tag="O0V33_000959"
     CDS             complement(18787..19647)
                     /locus_tag="O0V33_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCX8975616.1"
                     /translation="MLSVAIIGPGAVGTTIVYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLE
                     HIPFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIVHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            19720..20538
                     /gene="panB"
                     /locus_tag="O0V33_000960"
     CDS             19720..20538
                     /gene="panB"
                     /locus_tag="O0V33_000960"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCX8975617.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEKAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGIEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            20531..21382
                     /locus_tag="O0V33_000961"
     CDS             20531..21382
                     /locus_tag="O0V33_000961"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCX8975618.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITVVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDMYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDKVTQYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            21384..21767
                     /locus_tag="O0V33_000962"
     CDS             21384..21767
                     /locus_tag="O0V33_000962"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCX8975619.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIKNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            complement(21896..23578)
                     /locus_tag="O0V33_000963"
     CDS             complement(21896..23578)
                     /locus_tag="O0V33_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HCX8975620.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGAIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGITDTGFYRFWT
                     QGIFARWTDNPNIEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNTEYTPLYLNAENHTLNHAKISSAHVAQYDSEDKRQD
                     VSFKYTFDKDTELVGNMNLKLWVSTTDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSTIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGSKYDSQLIIPIVN"
     gene            23635..23743
                     /locus_tag="O0V33_000964"
                     /pseudo
     CDS             23635..23743
                     /locus_tag="O0V33_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23721..24308
                     /locus_tag="O0V33_000965"
     CDS             23721..24308
                     /locus_tag="O0V33_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCX8975621.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSLI
                     RHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ"
     gene            complement(24389..24838)
                     /locus_tag="O0V33_000966"
     CDS             complement(24389..24838)
                     /locus_tag="O0V33_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HCX8975622.1"
                     /translation="MTALFPYIAFENSKEALAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEDAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EQIKDHSSIEIELPFADQFWGGKMGIFTDKYGVRWMLHGQDYTAIQQ"
BASE COUNT         8576 a         3942 c         4755 g         7847 t
ORIGIN      
        1 gtgaatcaat ataattatta cattttgaga taaatcgaaa caggattcat aaaattaata
       61 attaggggga gcacaattga aaaaagagaa agttatggac tggacgacct ttatagggac
      121 agtagctgta cttctttttg cagttatacc tatgatggct tttccaaaag caagtgaaga
      181 tatcatcact ggtattaata gtgccatttc tgattcaatt ggttcgatat atttatttat
      241 ggggctggcg attttttgct ttgtaatgta tatcgcattt ggtaaatatg gtaatgtcac
      301 acttggtaaa gcaagtgata aaccagaatt taatacattt acatgggcgg caatgctgtt
      361 ttgtgcaggc ataggctctg atattttata ctggggcgtt attgaatggg ctttttacta
      421 tcaagttcca ccaaatggcg cgaaaagtat gagtgatgaa gcactccaat atgcgacgca
      481 atatggtatg ttccactggg gaccaattgc ttgggctatt tacgttttac cagcattacc
      541 aattggttat ttagtgtttg ttaaaaaaca accggtgtat aaaattagtc aagcttgtcg
      601 cccgatttta aaaggtcaaa cagataaatt tgtaggtaaa gttgtagata tcttatttat
      661 ctttggattg ctaggtggtg cggcaacatc actagcgtta ggtgtgccat taatttctgc
      721 aggcatagaa agattaactg gtttagatgg taaaaatatg attttacgtt cggccatttt
      781 attaacaatc acggttatat ttgccattag ttcatataca ggattgaaaa aaggtattca
      841 aaagttaagt gatatcaacg tttggctatc atttgtactt ttagccttta tatttattat
      901 tggaccgact gtttttatta tggaaacgac agtgacaggg ttcggaaata tgttgagaga
      961 tttcttccat atggcaacat ggttagaacc attcggtggt attaaaggtc gaaaagaaac
     1021 gaatttccca caagactgga caatattcta ctggtcatgg tggttagtat atgcgccatt
     1081 tatcggttta tttatcgcta gaatttcaaa aggtcgacgc cttaaagaag tcgtgctagg
     1141 aacaattatt tatggaacgc ttggatgcgt attattcttt ggtatttttg gtaactatgc
     1201 tgtgtattta caaatttctg gacagtttaa tgtaacacaa tatttaaata cacatggtac
     1261 agaggcaacc attattgaag tggtgcatca tttaccattc ccatcattga tgattgtact
     1321 attcttagta tctgctttct tattcttagc aacaacattt gattcgggtt catatatttt
     1381 agcggcagca tctcagaaaa aagtggtagg cgaaccatta cgtgccaatc gtttattctg
     1441 ggcgtttgca ttgtgcttat tgccattttc attgatgcta gttggtggtg aacgtgcatt
     1501 agaagtattg aaaactgcat caatactggc aagtgtgcca ttaattgtta tttttatttt
     1561 catgatgata tcatttttaa tcattttagg acgcgataga attaaacttg aaacgcgggc
     1621 tgaaaaatta aaagaagttg aacgtcgctc attgcgaatc gttcaagtat cagaagaaga
     1681 acaagacgat aatttataat tcaattaatg atgcataaga ttttgaattt tataagtata
     1741 tgattaatcg tgatattaag caaaaacggt aatttctatt tattgagata gaaattaccg
     1801 tttatttcgt ggacctattg cattgttttc atcatgcata atcatcattg tcgttgtttg
     1861 agtcaatttt aatttttaga atcagaagac tgttctggaa ttgggaaata tttaaaaatt
     1921 tcaccgcttt caatcgcttc ggttaactgt tctaaccatt cgtaataaac atgtgtatga
     1981 tcaagctgag ctttaatttt gtgtgcctct tgtgtttcag cttcagttaa atcactgctt
     2041 tcaagtaatg gattgataat agcttgagca tcttttactg cttcgacatt gatatcaatt
     2101 tcacgctgaa attttttagt gaaaaagttt cggaaaaaga tgaaaaagtc tttctcggcg
     2161 ataaaatgtt gtttgcggct tcctctcgta aattgttgtt taacaatatc aaattcctgc
     2221 aatttcttaa cgccagcact catacttggt ttgctcattt gcaattgatg acgcatttca
     2281 tcaagcgtca tactgccttc aaacaccata gtgccatata agtttcctac acttctatta
     2341 gtgccataca aatccattgt ctgtccaatt gaattaatta caatatcttt tgcttgttct
     2401 aattgttgct gtttgttctg agaacgagtc atcattgcac ctccgtacat cattttggtc
     2461 acgttaaaat aaatactaat acattataaa accttttcta aaaaaagaca ttaaaaatat
     2521 ttaaagcatt aaagttaaat gtttcgttaa ataaaaatct aacgaactta caaaacttaa
     2581 ttcttgagtt gttttgtaaa ttgacacatt tttcatttct atgctaacat aagtttgtaa
     2641 aattcgttaa ataaaaattt aacaaactta acggaggttg ttgaatggaa cttttaaaac
     2701 atttatctca gcgtcaatat attgatggtg agtgggttga aagcgcgaat aaaaatacaa
     2761 gagatattat caatccttac aatcaagaag tgatatttac ggtttctgaa gggacaaaag
     2821 aggatgcaga acgtgcaatc ttagctgcaa gacgtgcgtt tgagtctggt gaatggtcgc
     2881 aagaaactgc tgaaacaaga ggtaaaaaag tacgtgccat cgcggacaag atcaaagaac
     2941 atcgcgaagc gttagcacga ttagaaacat tagatactgg aaaaacgtta gaagaatcat
     3001 atgcagatat ggatgatatt cataatgtgt ttatgtattt tgctggatta gcagataaag
     3061 atggtggcga aatgattgat tcaccaatac cagatacaga aagcaaaatt gttaaagaac
     3121 cagtaggtgt agttacacaa attacacctt ggaattatcc gttattacaa gcatcatgga
     3181 aaattgcgcc agcgcttgct acgggttgtt cactagttat gaaaccaagt gaaattacac
     3241 cattaacaac aatacgtgtt tttgaattaa tggaagaagt tggtttccct aaaggaacaa
     3301 ttaatcttat tcttggtgca ggttctgaag ttggtgacgt aatgtcaggt cataaagaga
     3361 ttgacctagt atcatttaca ggtggcattg agactggtaa gcatattatg aaaaatgctg
     3421 ctaataatgt tacgaatatt gccttggaac ttggcggtaa aaatccaaac attatctttg
     3481 atgatgctga ttttgaattg gcggtagacc aagcgttaaa tggtggatat ttccatgcag
     3541 gtcaagtttg ttcagcagga tcaagaatat tagtacaaaa cagtattaaa gacaaatttg
     3601 agcaagcact tattgatcgc gtgaaaaaaa tcaaattagg taatggtttt gatgctgata
     3661 ctgaaatggg accagtgatt tcaacagaac atcgtaataa aatcgaatct tatatggatg
     3721 tagctaaagc agaaggcgca acaattgctg ttggtggtaa acgcccagat agagacgatt
     3781 taaaagatgg tctattcttc gagccaacag tcattacaaa ttgtgatacg tcaatgcgta
     3841 ttgtacaaga agaggttttc ggacctgtcg ttactgtaga aggctttgaa actgaacaag
     3901 aagcgattca attagcgaat gattctatat atggtttagc aggtgctgta ttttctaaag
     3961 atattggaaa agcacaacgc gttgctaaca agttgaaact tggaacggtg tggattaatg
     4021 atttccaccc atattttgca caagcgccat ggggtggata caaacaatca ggtatcggta
     4081 gagaattagg caaagaaggc ttagaagagt accttgtttc aaaacacatt ttaacaaata
     4141 caaatccaca attagtgaat tggtttagca aataaaaatt agataaggtg agtgccattg
     4201 taagaacaca agacactcac tttgttttgt ataagtggcg aaatgttgat tgataatttg
     4261 gactaaacgc aaaatgaatc atagattatt tcatgactgt tagtaacaat cataaaagga
     4321 aaatcgagtg ttttggttag ctaagtttag caattcaacg ataaccaatc agccactaac
     4381 aaatatttca tgcaatactc actttgaaat acaacaaact ttggaggtca taacgatgag
     4441 taacaaaaat aaatcatatg attatgtcat cattggagga ggcagtgcag gttctgtact
     4501 aggtaatcgt ctgagtgaag ataaagataa agaagtctta gtattagaag cgggtcgcag
     4561 tgattatttt tgggatttat ttatccaaat gcctgctgcg ttaatgttcc cttcaggcaa
     4621 taaattttac gattggattt attcaacaga tgaagaacca catatgggcg gtcgtaaagt
     4681 agcacatgcc cgaggtaaag ttttaggtgg atcaagttcg attaatggca tgatttatca
     4741 acgcggtaat ccaatggact atgaaggctg ggcagaacca gaaggtatgg aaacttggga
     4801 ttttgcgcac tgtttaccgt attttaaaaa attagaaaaa acatacggtg cagcgcctta
     4861 tgataaattt agaggccatg atggaccaat taagttaaaa cgagggccag caacgaatcc
     4921 tttattccag tcattctttg atgcaggtgt tgaagcaggc tatcataaaa cacctgatgt
     4981 gaatggattt agacaagaag gttttggacc gttcgatagt caagtacatc gtggtcgccg
     5041 aatgtcagct tcaagagcat atttacatcc agctatgaag cgtaaaaact taaccgttga
     5101 aacacgtgcc tttgttactg aaattcatta tgaaggtaga agagcaactg gtgttacgta
     5161 taagaaaaat ggcaaactac ataccatcga tgctaatgaa gtcatcttgt caggtggggc
     5221 attcaatacg ccacaattac tacaactgtc tggtatcggc gattcagaat tcctaaaatc
     5281 aaaaggcatt gagccacgtg ttcatttgcc tggtgtgggt gaaaactttg aagatcactt
     5341 agaggtatac attcaacata agtgtaagga acctgtatca ttacatccaa gcttagatat
     5401 caagcgcatg ccatttattg gtttacaatg gattttcaca cgtactggtg cagcagcatc
     5461 taaccatttt gaaggtggcg gatttgtacg ttcaaataac gaagttgatt atcctaactt
     5521 aatgttccat ttcttaccaa tagctgtaag atatgatggt caaaaggcag cggtcgcaca
     5581 tggctatcaa gttcacgtag gaccaatgta ttcaaactct cgtggcagct tgaaaatcaa
     5641 atctaaagat ccattcgaaa aaccaagtat ccgctttaat tatttatcaa cagaagaaga
     5701 taaaaaagaa tgggtagaag caattcgtgt agcaagaaat atcttatctc aaaaagcaat
     5761 ggatccattc aatggtggag aaatttcacc aggaccagaa gtgcaaacag acgaagaaat
     5821 tcttgattgg gttcgtagag atggtgaaac agcattacac ccatcttgta gtgctaaaat
     5881 gggtcctgct tcagatccaa tggcagtagt tgacccgtta acaatgaaag ttcatggtat
     5941 ggaaaattta cgtgtagttg atgcatcagc tatgccacgt acgacaaatg gcaatataca
     6001 cgcacctgtt ttaatgttag ctgaaaaagc agcagacatt atacgtggca gaaagccttt
     6061 agagccacaa tatattgatt attataagca tggtgttcat gatgaaaatg aaggtgcaat
     6121 cgaagtaaaa ccatacgcta aataaattga tatagtagac ttttagtctg gcatctattg
     6181 tattggtgcc aggctttttt gcgttttatc taaatgagaa gttaatgtgt tgttcaaaaa
     6241 cggtgtgtga gaatatgaac tgaaaataga gttaattgta cattgtgatg attaattgac
     6301 ctaattgaca gacattaaaa gagtgtacaa ccttaaaaag ttcactaaat agacatttaa
     6361 ttgtgttttc ttgaaagctt tcgtggtagg atttatataa gtaaattgag agaaggaata
     6421 ctatgagctt ttacgaatat atacaaacat ttaaagatga taaaacacca ttaggcgaat
     6481 tagcgatttg gattaaagaa gatgattcat tcccaaaaca agagaagcta acagaaaaca
     6541 ttttgtctta ttttcatcaa atgtccaata tagatcatga gtttttagaa attgtaaaaa
     6601 gatcactttc tctgtatgat caattaaaat cgtaaaatta gagataaagt tagctaaaat
     6661 acaaggccgg taatttcata gatgaagttg tcggctattt tcatgactaa tgttaaataa
     6721 tgatcatgga attgaagaca caaaaataat gggcgacgcc tgtccccatt tgtatggaat
     6781 attaatactt tttacaaagc cttatctttt aaaaagaagt caaactatga ttgtaattaa
     6841 taaatacggg aatgaattag taatagatat aaagtaatat gaccaaaaat ataaatgaaa
     6901 tgaaggcatt tattatgaac atttatgatg aatatagaag ttatgtaata gaagaactgg
     6961 atgattgtct cacaatacaa aaaaataatg acactgcata ttacgatgtg ctagaagcga
     7021 taaatgattt atcaaatgat acattatgtg tgttaaatca tttatatatt aacgaaggac
     7081 aagaagaaac attcgagcaa aaatttttgc aacgtaataa gcatttgcaa aacgttgatg
     7141 ggtttaaagc attaaggttt ttaagaccta gaacagctgg tagacactat atcattatta
     7201 cgctgtggga aaataggcaa gcattttatc attggcaaaa ttcagctgag tacaagcata
     7261 cacacaagca tcgtggcact tcgaaaggtg cagatgtaaa aataattaat cgtgaattat
     7321 catacaatat tcgtattgaa ttagctgata tgctctagcc cgagatatca accctcaatc
     7381 atttgaaagt ccttgaatgc tatgtcatgt caaagcgtag tgtcgaggac tttggtattt
     7441 aaccctattt aattgaaaat tctgtaaaga taggcgtatg atatatataa ttcatttata
     7501 aaattacaat gttcatcaca ggaaaagtgg atgtatgaaa ggaagttagt tatgcaaaag
     7561 tcagctctat tcgcgcctga aaaatataac attatttcgg aaatcgaaaa atatagccac
     7621 ataccagata aaaaggcaat tttatatcat aatactgaag gcgaagatat atcagtcact
     7681 taccaacagc tcattgaaca gtctaataaa gtaggtaatg ttttagcatc gcacggttta
     7741 tctaaaggcg ataaagtact cattatgatg ccacgatcta ttgcgacata tgaattatat
     7801 attgctgctt taaaattagg tgttgcaatc attccatgtt ctgaaatgct aagaactaaa
     7861 gatttacaat accgtatcac acacggtgaa ataaatgctg ttattgcact agagccattc
     7921 acagttgaat ttgaaaaaat taaagaatat gatgcgttaa ctaaatttgt tattgccggt
     7981 caaaaagatg gatggatttc acttgaagct gaaaaagaaa acgcaagtaa caaactagaa
     8041 atggcagata cgacgcgtga tgatatggcg attctgtcat atacatctgg tacaactggc
     8101 aatcctaaag ccgttacaca ttgtcatgga tggggatttg cacatttaca aatggcacca
     8161 aaacattggt tatgtattaa tgaagatgac ttagtttggg caacagcagc accaggttgg
     8221 caaaaatggg tatggagtcc tttcttatct gtattaggtt caggtgcaac tgcttttgta
     8281 tttaatggaa gattcagccc tgagacatac ttagaattgc ttcaaaaata tcaaattaat
     8341 gttttatgtt gtacaccgac agaatatcgt atgatggcta aattacagaa cttaaatgat
     8401 tatgacttaa cgcatttaca tagtgccgtt tctgcaggtg agccactaaa tcgagaagta
     8461 gttgaacaat tcaagaaata ctttaattta actgtaagag atggatatgg ccagactgaa
     8521 agtaccttgc ttataggctt tttaaaagat acaccacaaa ggattggatc aatgggtaaa
     8581 ggcattccgg atagttcggt cactgtagta gatgatgagg ggaatagcgt acctgctaat
     8641 acgaaaggga atattgcagt accacttgat ttgccagcac ttttcaaagg atattataaa
     8701 gagccagaac gtacggcgaa agcgcaaaca ggtaaatact acatcactgg tgacttagct
     8761 catgtagatg atgatggtta tttctggttt gaaggacgtc gagatgacat tattattagt
     8821 tcaggctata cgattgggcc ttttgaagtt gaagatgcgt taacgaatca tccagccgtt
     8881 aaagaatgtg ctgtagtagc aaaacctcat gatatacgag gaaatattgt taaagcattc
     8941 gttattttac aggaccatac tgcgggtgat gatacgctag ttaaagaact acaacaattt
     9001 gttaaaaatg aagttgcacc atataaatat ccaagagaga ttgaattcgt tgacgactta
     9061 ccaaaaacaa actcaggtaa aattcgtcgt gttgaactac gagatgctga aatagaaaaa
     9121 tggcaacaac aaaaagatag caatcaataa acatagcaaa gcagtccaat gcgtaaggta
     9181 tgatgatacg cactggactg cttgtttatt tatgatgttc tttgtcgtct agttataaat
     9241 attaaaatta gtttagaaac aagcgcaacc agtattaaga atgagaaaat ataagatgtt
     9301 aaacctcgaa tatcccagtt taataaaaaa acattactat caccggcatt aagttgaaac
     9361 tcagtatgtg ttaaaacgat aatcacaatg cctataagtg caagattcaa aataatttac
     9421 aatattaaag gtaattgaag tgattttctt ggatgcatag gcattgaatc tcctttttat
     9481 gattgatttt acacttatat tgtatcaaaa tagagtatag gtttaaaatg ctataagcca
     9541 aatgtagatt aaaacaatcg ttagctattt gttgtcaaat aattatgatt gatttgatat
     9601 ttatcttcta tgaataaaag atttaatata ttgcaaagtt aatgaataat tataatttac
     9661 atttaccact gcttgcttta acatatacat gtaagcacta actatagtat tttgttacag
     9721 cgtatcaatt aaaacaggaa gttatctatg gcgttgatat tttaaagaaa tatgtcagtt
     9781 ttatgtaact ttcatattga tgatacacat ttacaatttt tggagaggtg aaaccaatgg
     9841 ctaagtctaa tagtaaagac atcgttttaa ttggagccgg tgtacttagc acaacatttg
     9901 gttcaatgtt aaaagaaatt gagccagact ggaatatcca cgtttacgaa cgcttggatc
     9961 gtcctgcaat cgaaagttca aacgaaagaa ataatgctgg tacgggtcat gcagcattat
    10021 gtgagttgaa ctacacagtt ttacaacctg atggttctat cgacatcgaa aaagcgaaag
    10081 tgattaacga agagtttgag atttcaaaac aattctgggg tcacttagtg aaaagcggta
    10141 gcatcgagaa cccaagagaa tttatcaatc cattaccaca catcagttat gttagaggta
    10201 aaaacaatgt taaattctta aaagatcgtt acgaagcgat gaaagctttc cctatgttcg
    10261 ataatatcga atatactgaa gacatcgaag taatgaaaaa atggattcca ttgatgatga
    10321 aaggccgtga agataaccct ggtatcatgg cggcaagtaa aattgacgaa ggtacagatg
    10381 taaacttcgg tgaattaaca cgtaaaatgg ctaaaagcat tgaagcacat ccaaatgcta
    10441 ctgtgcaatt taaccatgag gttgttgatt ttgaacaatt atcaaatggt caatgggaag
    10501 ttactgttaa aaatcgccta actggtgaga aattcaaaca agtaactgac tatgtattca
    10561 tcggtgctgg cggtggagca attccattat tacaaaaaac aggtatccct gaaagtaaac
    10621 atttaggtgg attccctatc agtggtcaat tcttagcttg tacaaaccca caagttattg
    10681 aacaacacga tgccaaagtt tatggtaaag agccacctgg tacaccacca atgactgtac
    10741 ctcatttaga tacgcgttac attgatggtc aaagaacatt attatttgga ccatttgcta
    10801 atgttggacc taaattcttg aaaaatggtt ctaacttaga tttattcaaa tcagttaaaa
    10861 catacaacat tacaacttta ttagcagcag ctgttaaaaa cttaccttta attaaatact
    10921 catttgatca agtgattatg acaaaagaag gttgtatgaa ccacttacgt actttctatc
    10981 cagaagcacg taatgaagat tggcaattat acactgctgg taaacgtgta caagttatca
    11041 aagatacacc tgaacacggt aaaggattca tccaattcgg tacagaagtg gttaactcac
    11101 aagaccacac tgtaatcgca ttattaggtg aatcaccagg ggcttcaact tcagtttcag
    11161 ttgcgttaga agtattagaa cgtaacttcc ctgaatacaa aactgaatgg gcacctaaaa
    11221 tcaagaaaat gattccatca tacggtgaat cattaattga agacgaaaaa ttaatgagaa
    11281 aaatccgtaa acaaacttca aaagacttag aattaggtta ctacgaaaac taaattctaa
    11341 tagttatatt taaaatcgga caactgagaa atcttaaaga tttttcagct gtccgatttt
    11401 tttacgtaat ctaatacata cggcaaaatc aactttaatc aaaaagactc atacacaatg
    11461 cctttaaagc acatgtatga gtccttttta gtagtttata tcaaaaaata cgtttaatgt
    11521 ataaattagt ttttgtttac agatgcgtcg tagattgatt ctacagcgtc acctaatgct
    11581 ttatcgaatt cttctttaga ttgtccagct cttaaatcac tagctaatgc gcgtgagaag
    11641 cttgcgataa gttcagcgtt atctttaagt aattcattag ctttttctct gctgtaacca
    11701 cctgataata caacgatacg aacaacgttt ggatgctcag ctaagtcttt gtataagtta
    11761 gctttagtag gaattgttaa ttttaacata actaattgat cagcatttaa gctgtctaaa
    11821 ccttttttaa gctcagcttt taatactttt tcaatttcag ctttatcttt tgcgttaata
    11881 ttaacttctg gttcgataat tggcactaac cctttagcaa taatttgttt agcgacttca
    11941 aattgttgtt caacaacgtc tttgatacct tgttcattta attctaaaat attagaacgc
    12001 atttttgtac caaaaatgtg acgctcgttt gcacgatcta ataagctatc taagttgtcg
    12061 attggtttca ttaattgaac accattttgc tcttcagcaa gacctttgtc tacttttaag
    12121 aacggaacaa cacctttgtc agctaagtaa tctgcagtgt atttgccttc tacttcgcga
    12181 tccattgttt gttcgaaaag aatagcacct aaaattttat ctggtgagaa tgaaggtgaa
    12241 gttaccacac gtgtacgcat atcgtgaaca agttggaaca tttcgtcttc attgctatat
    12301 tgatcttcgt ttacaccata ttctttaagt gcttttggtg tactaccacc actttggtct
    12361 aatgcggcaa taaagccttt tccatttttc attttttcta attgctcttt attcatactt
    12421 tccactcctt aacttttcaa tacacctcca gtatgataaa aatgagaaca tttctcaagt
    12481 cataaacctt gaaaagtgta taaaatgtga aaaataattg tcagtttaat taaaaatatt
    12541 attttattct aggtatgact aacgccatta atgacatgaa gaaaatatgt gtaataatcc
    12601 aaccgattaa ttctgtcaca ctaaattgaa aaattggacg ttgcgcaata aatactaaaa
    12661 agggatacaa tgctataaat aagaaaacta aagggatata acatagatag taaagccttt
    12721 tagaagtatg aaacagcaac acaaatacaa aataaatgac gctaccaatt agtaagtgac
    12781 atattatttc tagagtatat ggtgtattgt cagaaggaat tagaaaatct aaattcaata
    12841 acaaagcttt taaatgtgat tgattgctta ttatgtcaaa caacatggtc atccatagca
    12901 acatgaatga tatgaaaata gcagcaatga aatggaacaa tacctttaaa cgcattcata
    12961 tcacctcaga gtcaaatgga atatagtaaa caattcccta atcgaacaaa attatgcgca
    13021 taaataaagt agattgatat aaaattctta attatcagaa tatatttaca aatctgaatt
    13081 ttattagtat attggatagt attcataatg acatgacagt attttcacgt gatatcaaat
    13141 cgaatgtggt tatcgatgta gggaaggtaa ttatgtgaat aaggtctgtc aatgggaggg
    13201 gaatgtatat gagtaaagca catcaattaa ttcaagagga tgaacattat tttgcgaaat
    13261 caggacgtat taaatattat ccgttagtta ttgatcatgg atatggagca acattggttg
    13321 atattgaggg aaagacatat atcgatttgt tatcgagtgc gagttctcaa aacgtaggtc
    13381 atgcacctag agaagtaaca gaagcgataa aggcacaagt tgataaattt attcattata
    13441 caccagcata tatgtatcat gaaccgttgg tacgtttagc taagaagctt tgtgagattg
    13501 cacctggaga ttttgaaaaa agagtgacct tcggattaac tggatcagac gcaaatgatg
    13561 gcatcattaa atttgccaga gcatatacag ggcgtcctta tatcattagt ttcactaatg
    13621 catatcatgg ttcaactttt ggctcgttgt ctatgtcagc tattagttta aatatgcgca
    13681 aacattatgg tccgttattg aatggctttt atcatattcc gtttccagat aaatatcgtg
    13741 gtatgtacga gcagccacaa gctaattcag tagaagaata tttagcaccc ttaaaagaaa
    13801 tgtttgcgaa atatgtacct gctgacgaag tagcatgtat tgttattgaa acgatacaag
    13861 gcgatggtgg acttttagaa ccagttccag ggtattttga agcgttagaa aatatttgtc
    13921 gtgaaaatgg tattttaatc gctgtcgatg atattcaaca aggttttggg agaacaggta
    13981 catggagttc agtctcgcat tttaatttta cgcctgattt aatcactttc ggaaaatctt
    14041 tagcaggtgg tatgcctatg tcagcaattg ttggacgcaa agagattatg aattgtttag
    14101 aagcaccagc acatttattt acaacaggtg ctaatccagt tagttgtgaa gctgcattag
    14161 ccacaattca aatgattgaa gatcagtcgc ttcttcaggc tagtgcggaa aaaggggaat
    14221 atgttaggaa acgaatggat caatgggtat ctaaatacaa tagtgtaggc gatgttagag
    14281 gtaaaggcct gagcattggt attgatattg tttccgacaa aaacctcaaa acacgtgatg
    14341 ccagtgcggc acttaaaatt tgtaattact gctttgagca tggcgtagtt attatagctg
    14401 tagcaggaaa tgtgttgcga ttccaaccgc cattggtaat aacatatgag caattagaca
    14461 cggcgttaaa cactatagaa gatgcactga ctgctttgga agcaggaaac ttagatcaat
    14521 atgacatatc tggacaaggt tggtaatagc gattatctta atataaaata aaaaatcatt
    14581 tccacatctg gatgttaatc agatgggaaa tgattttttt aatttttatt ttggtgggtg
    14641 gtattcagct acgtcatttt tcttagaatg tctaagtcca taacttaaat atagaatgat
    14701 accaacaata aaccaaatta aagtgtataa tttcgcttcg aatcctaatc cccagaatac
    14761 tagcaatact aaaacaaatg taactgctgg taacacagga tataaaggta atttaaacgc
    14821 aggaattggt aaatctttac cttcacgctt tctcaaacga tacattgcta atgaaacgaa
    14881 cataaatgca acaagtgtac cggctgaaat taattgtgct aaaaatgcga atgggaacat
    14941 agaaccaatt aaaacaccaa taatagtaag tataactagt gcacgattag gtaaatgttt
    15001 gtcgtttaag tggcttaacc atgaaggtag taagccgtca cgtccaaatg aataaagtaa
    15061 acgtgagcct gctaacatca taccaattaa tgctgtaaac ataccgataa cagagatagc
    15121 ttgaacaata gctgctacaa caccatgacc actttgacgt aaagcccaac caacaggttc
    15181 agcattgttt gcgtattgtg agtaatggaa cataccaact aacacaagtg ctacagcaat
    15241 aaataatacg atagcaatac ttaaagaacc aagaatacct ctaggcattg tcttttgagg
    15301 gtcaagtgct tctgctgagt ttgctgcgat agaatcgaaa ccgatatacg ctaagaaaat
    15361 cattgaaaca ccagcatata tgccttgcca tccaccaaag tcacctgtag cagttacttt
    15421 gtgttctgga ataaatggca catagttact aacatttatt gctgttaaac ctacgattac
    15481 aaataaaata atagctaata cttttaaaat aactaaaatg ttttccatac gtgctgcttc
    15541 cgacatacca cgtgatagta ataatgcagt taataaaata acgatagcag caataatatc
    15601 gataaaaccg ccatttgtac caaatggatt tgataatgct gcaggtaatt cgatgccaat
    15661 tggtttcaca agtccgcgta aattcgctga gaatcctgat gcaacaaagg ctacggcgat
    15721 aaaatattca gctaataaag cccaaccggc aacccatcca aagaattcac caaataatac
    15781 attgacccaa gaataggctg aacctgcaaa tggcatagcg gcagccattt ctgcataagt
    15841 aaatgcaact aaaccagcaa caatagcagc gagtaagaat gataacgcaa cggccggtcc
    15901 tgcatgttct gcagcaacaa tgccaggtag cgtaaagata gatgtcgata caattgttcc
    15961 tacacctaaa gctaagaaat cacgcacccg aagtgtacgc tttaaatgac catctttatt
    16021 ttgatagata gccggatcct cttttcgtgc tattttattg aaaaaacttc ccataaactt
    16081 tcctcccaaa ccttcataaa caattctata cggtgttttt taatatgtta tatcatagca
    16141 caaataatca atattttgtc taaaaattct gaaaaatcac aactttatgt tacttattaa
    16201 tgacttgtct tgataacatc catagatttt ttaaatgata aaactgatta taacagatat
    16261 taaatgaata aatactattt ttgctaattt tctaacaatt ttgcgcatta tatttttaaa
    16321 atcaatttca tgtttatgtc tgattgccta gtttgtatga aatgtaattc tttgactata
    16381 aaaaggtgca tcaaatatga tgagagtaag gcgaaaaata ggaggttata tagtaagtca
    16441 tttaagtcga atagccatcc tttttaaaat gaaaaaaata gaaagctcaa tagtttgtta
    16501 aagcctttaa atagcgtcat tacgcaattt tagaatgcta aaaattgtca caaatttcaa
    16561 attacgtgct aaagcttgag atatcaatat ttattggcgt tagagtgtaa tttgactccg
    16621 cttacatctc agttttatgt ttgtaatttg gtagcataat attataataa aataaaattg
    16681 ttaatcttta atttcagtat agatattttt acgtgtagtc acgtgtaaaa taaattcaat
    16741 taggttagga gacataatta tgaaaacatt tggtaaaaag gttgtattaa tcggagatgg
    16801 atctgtagga tcaagctatg cctttgcaat ggttacgcaa ggtgttgctg atgaatttgt
    16861 aattattgac attgcaaaag acaaagtaaa agcagatgtt caagatttaa accatggtac
    16921 agtccacagt ccttcaccag ttgatgtgaa agcaggtgaa tacgaagact gtaaagatgc
    16981 agatttagtt gttattacag ctggtgcacc tcaaaagcca ggtgaaactc gtttacaatt
    17041 agttgaaaaa aatactaaga ttatgaagag cattgttaag agtgtaatgg atagcggctt
    17101 tgatggatat ttcttaatcg cggcaaaccc tgtagacatt ttaacaagat ttgtaaaaga
    17161 atatactgga ttgccagcag agcgtgttat cggttcaggt actgtattgg acagtgcacg
    17221 tttacaatat ttaattagcc aagaacttgg tgttgcacct tcaagtgttg acgctagtat
    17281 tattggtgag catggtgata ctgaacttgc agtttggtca caagcaaatg tagcaggtat
    17341 ttcagtatat gacacattaa aagaacaaac tggtagcgaa gctaaagcgg aagaaattta
    17401 tgtaaataca cgtgacgctg cttatgaaat tatccaagct aaagggtcaa catactatgg
    17461 tattgcatta gcattgatgc gcatttcaaa agccatttta aataatgaaa ataatgtctt
    17521 aaatgtttct atacaattag atggtcaata tggcggtcac aaaggcgttt acttaggtgt
    17581 accgacattg gttaaccaac atggcgcagt taaaatttat gaaatgccat taagtgcaga
    17641 agaacaagca ttattcgaca aatcagttaa aacattagaa gatactttcg attcaattaa
    17701 atatttatta gaagactaag cctattttaa gtattaatta gaaatatatt aatggtaaga
    17761 ggatctatta gtgttgcggt aacatgtggc actgatagat ctattttttt agcaagcatt
    17821 gatagaggga atacttttgc gtaaaataat aattgaataa agtattatga ctcgtgaaaa
    17881 tttagtgaca tgaatagaaa tgtcgaggcg tcattgagta taaaggaaag tagttctgca
    17941 ttaatcacga agtagagcat gacaacgagg aataactata gggagatggt tttggaatga
    18001 cgaatgtctt gtatcaacat ggtacattag gtacgttaat ggctggctta ctagaaggaa
    18061 cagctacaat taatgaattg ttagaacatg gaaatttagg gattgcaacg ttaacagggt
    18121 ctgatggcga agtaatattt ttagatggga aggcatatca tgctaacgag cataaagaat
    18181 ttatagaatt aaaaggcgat gagaaagtac cgtatgcatc gattactaat tttaaagcga
    18241 gtaagacatt tccattgcaa caattatcac aagatgatgt attcgcacaa attaaaaatg
    18301 aaatgttaag tgagaattta ttttcggctg ttaaaattta tggcacattt aaacatatgc
    18361 atgtacgaat gatgcctgct cagcaaccgc catatacacg tttgattgat tcagcacgca
    18421 gacaacctga ggaaaaaaga caagatattc gtggtgccat tgttggattt tttacaccag
    18481 aattatttca tggcgtaggg tctgctggtt ttcatataca ttttgcggat gatgaaagag
    18541 cttatggtgg acatgttctt gactttgaag tggatgacgt tgtcgttgag atacaaaact
    18601 ttgaaacatt ccagcaacat ttcccggtaa ataacgagac gtttgttaaa gcgaaaatag
    18661 actataaaga tgtggcagaa gaaattagag aagctgaata gtctaatata attaaaagac
    18721 cttagcgata ttaggaacag gtggttctaa atgcatcgtt aaggtctttt tatattatat
    18781 atgtgcttac atattttgtt gatacgcgcg taaaaagcta taaatagtat caagatatgg
    18841 ggtgtcaaga ttgtgttcac gtgcacgtcg ataaataaag ccttgaatcg cttcgacttc
    18901 aagaggttgc tgatgcacaa tgtcgtaata catgctcgtt cccatttcat cgggataacc
    18961 ttgatatata gtcataatag tatcgacagt ttgttctgaa aagtttaacc cctctgcctg
    19021 tgcaacacga caaccatcta gtaatagctg tctacacagt gtacgtattt caggattatg
    19081 catgattgca actgtctgtc taccgagtgc tgtgatagaa ttaatgccca agttaactaa
    19141 caatttatac caaatagctt gttgaatatt tgcttctagt acaatgtcga tttgactgtc
    19201 ttggaccaaa tctctaaatt gtcgagttaa tgcattatct tgtatacgta attgataatc
    19261 tctgaagtgc gtaacaacat cgcctttctt ttgaccactt atatagacaa ctgcttggca
    19321 tacgttttta aatggaatat gttcgagttg accataacca ttttgggcta aaatgatgag
    19381 cgtgtcctca tgtgcaagat gagttaaatg aggaataact gcatcaagtt gatgtgtttt
    19441 gactgcaata ataataacat caaatgtgtt tgtgacatct tcataacctt ttacaacaat
    19501 atcttgtgca ggtgcatgtg gtacagtata atatgttatt gttttggcgt gtctcccgat
    19561 aagcgttgta tgaggcaatg attgttgtaa ttcatagaca atagttgtac cgacagcgcc
    19621 tgggccgata atcgcaactg ataacatctt cttctttcct ttctataaaa gattcattat
    19681 actatttcta ataatactgc aaaaagaggt tgaataacat tgaaaacagt aagtcaatta
    19741 atagatatga aacaaaagca aactaaaatt tctatggtaa cagcttatga ttttccaagt
    19801 gctaaacaag tggaagcagc gggtattgat atgattcttg ttggggattc acttggtatg
    19861 actgtattag gttatgaaag taccgtacaa gtgacgttag cagatatgat tcatcacggt
    19921 cgtgcagtaa gaagaggcgc accaaataca tttgtagttg ttgatatgcc gattggtgca
    19981 gtaggcatct ctatgacaca agacttaaat catgctctaa agctttatca ggagacgaat
    20041 gcaaatgcaa ttaaagcaga aggagcgcat attacaccat ttattgaaaa agcaacggca
    20101 attggtattc ctgttgttgc acatttagga ttaacacctc aaagtgttgg cgttatgggg
    20161 tataaattac aaggtgcaac gaaagaagca gcggagcaac ttattttaga tgcgaaaaat
    20221 gtagaaaaag ctggtgcagt tgcgttagta cttgaagcaa ttccaaatga tttagcagag
    20281 gaaattagca agcacttaac aattccagtc attggtattg gtgcaggaaa aggtacagat
    20341 ggtcaagtat tggtttatca cgatatgtta aattatggta ttgaacataa agcgaaattt
    20401 gtgaagcaat ttgctgattt tagtgttggt gtggatggtt taaagcaata cgatcaagaa
    20461 gttaaatctg gtgcatttcc atcagaagaa tatacgtata aaaagaaaat tatgaatgag
    20521 gttaataaca atgactaagc tgattactac ggtaaaagag atgcaacaca ttgttaaagc
    20581 agccaaacgc tcaggcacaa cgattggttt tatcccaaca atgggtgcct tacatgatgg
    20641 acatttaacg atggtacgcg aatcagttag tacaaatgat attacagttg tgagtgtatt
    20701 tgttaatcca ttacaatttg gtccaaacga agatttcgat gcttatccga gacaaattga
    20761 taaagattta gaattagtat cagaagtagg ggcggacatt gtttttcatc ctgcagtaga
    20821 agatatgtat ccaggtgaat taggtattga tgtcaaagta ggtccattag ctgatgtgtt
    20881 agaaggagcg aagcgtccag ggcattttga tggggtggta acagtagtta ataagctatt
    20941 taatattgtt atgccggatt atgcttattt tggtaaaaaa gatgcccagc aattggctat
    21001 tgtcgagcaa atggtaaaag acttcaatca tgccgttgaa attattggta ttgatatcgt
    21061 tcgagaagca gatggtttgg cgaaaagttc aagaaatgtt tatttaacgg agcaagaacg
    21121 acaagaagcg gtacatttaa gtaaaagttt gctattagca caagcgttgt atcaagacgg
    21181 tgaacgtcaa agtaaagtaa ttatagacaa ggttactcaa tatcttgaat cacatataag
    21241 tggacgcatt gaagaagttg ctgtttatag ttatcctcaa ttagtagaac aacacgaaat
    21301 aactggacgc atatttatct cgttagctgt caaattttca aaagcgcgtt taatagataa
    21361 tataataatt ggagctgaat aaagtgataa gaacaatgat gaatgctaaa attcatagag
    21421 caagagtgac tgagtcaaat ttaaattatg taggtagcat tacgattgat tcagatatat
    21481 tggaagcggt agacatcttg ccaaatgaaa aagtagccat cgtgaataat aataatggtg
    21541 cacgttttga aacatacgta atagctggtg aaagaggtag tggtaaaatt tgtttaaacg
    21601 gtgcagcgtc aagacttgtt gaagtaggcg atgtagtcat aatcatgact tatgcacagt
    21661 tgaatgaaga agaaattaaa aaccatgcac ctaaagtagc agtaatgaat gaagacaatg
    21721 tcattattga aatgattcat gaaaaagaaa atacgatagt gttataaaat taaatagcca
    21781 tcaataataa aaagggccac accttggttt aagattccaa gcgtgtggct ttctcttgtt
    21841 atacagtcct atcactatgt cacttttaat caaaatcgac cgtaattgtt gcatatcaat
    21901 taacgatagg aatgattaat tgtgaatcat atttactacc agtataaatc atgtgcatgc
    21961 ctttatttac ggtttcttca tgttcataac gtgttttcat gccaggggta ctattaccaa
    22021 ttacaatttc actacctttt acaacaactt ccaatgtttc gccttgttta aatagcgtgc
    22081 ctgaaggtaa caattcgatt tcaacaggta caatctcatc ttgtgacaac tttaattctg
    22141 tttcatgttt atgccaaggt tgcgcgattg tggatttttc ttgatctaat tcacgatgtg
    22201 atacgcgtaa ccaaccagta gctacttgac cattttcaat atgattaaaa tcagggaagt
    22261 taacttcatt accacgacga tctaactttt taatacctgc aaataaatcc atatcgtctg
    22321 agtctgtagt gcttacccat agttttaagt tcatgtttcc aactaactca gtatctttgt
    22381 caaacgtata tttaaaactt acatcttgtc gtttatcttc agagtcatat tgtgcgacat
    22441 gcgcgctact gatctttgca tgattcaatg tgtgattttc agcattcaaa tacaatggtg
    22501 tatattctgt gttaggtaaa gggaacgctg acgctgattt gaattcgcct ttataaaatt
    22561 gatctctaac ttcataaatg acatgaggcg tatctttcca atcgttattt tcttctttaa
    22621 ggtaaaaatc aaagaatgat ttttggcgtt cgagattttc tctagcatag taactttccc
    22681 actctttacg tccatgcaca tatagccatt tttcttctga tgctgcttgt ttaaatcctt
    22741 caaaagagcc acggttgtgc aaaccttgtg tagaccaact agcacatgtt agtagaggtg
    22801 ttttaatttg tgataatggc acttgacgct gtttccaaaa atcatcgaac aaagggtgtt
    22861 cttgttgtgc ttgaatcaaa tcttcgatat ttggattatc tgtccatctc gcaaaaatac
    22921 cttgagtcca gaaacgataa aagccagtat ccgttatacc tccgtgaaag gctacttctc
    22981 tatacatatc atttaagcct tcccaaggaa tcattgcttt taaatgtggt ggatttaatg
    23041 atgcgaccca ccattgagtc accgcaagat aagaaacgcc atttgtcccg atgtttccat
    23101 tactccatga ctgatttgct gcccactcaa tcacttcgta ataatcttcc gcttctcttt
    23161 ttgaccatgg agataagacg ccttttgatt tgtcactacc gcgtaatgca actttaacta
    23221 caacataatc atttggcacc caaaatcctg ggtctggtga ttcttcaggt gtaaaactag
    23281 atgtcggaat ggcgcctaat gttggccaaa gggcacccat atttgtgatt ttaggcttat
    23341 tatctttacc gtaagtatct gcagacataa ctacagggaa tttgccatct ttatttggtc
    23401 tgaaaatatt aatatataat ttttcgccat cgcgcatttc cacagtgaca tctttttcca
    23461 taatcatttc ttgatttcca tattgaacac tgtcgacaac gatgtggtta ataccggctt
    23521 tcacttcatt gacatgagtt acagttaatt ttggatttcc tagtaaatgt tggttcattg
    23581 ttatcacctt tttctgtttg tttgtatcac ctatattaaa caggtaaaca ttgtgtgcca
    23641 atcatcaatg ttggagattt gtaggataat gaacataaca tttataattg tttataatca
    23701 ttggggagga cttgatacaa atgactatag atcgacgtat tagaaaatca caagttgcaa
    23761 tgagaaatgc atttattgaa cttttacatc aacaccaatt agaagagatt actgtgcaac
    23821 aaattgcaga tttagctgat gttaaccgta gtacatttta tacgcattat tatgataagt
    23881 atgatttgct tgaaaaatta gaggaccagc agttggagga aataagagac ttcattcatg
    23941 atgagaagtt aaatggagca gttaagttat ccacggataa catacatcaa attatgaccg
    24001 ttttaataga aatgatagaa aaagacatcg cattttatca attgatgttt cgtatgggta
    24061 aagattcaaa tatccacgaa aaactttatg atttaattat gtgtcatttg caacgttata
    24121 aaaatgaaaa tgatcaaatt aatggtatac cattttccta tttcatgagt tatgtttcag
    24181 gtgcgggatt gtctttgata agacattggg tagaagatcc aaaccgtata gacaaagttg
    24241 acttaataag gcattttaat acgattgtca atcaagggcc agcaacgata attaaaaata
    24301 tacaataaaa aaaggcaagc catgaattaa gagtggcttg ccttgctaaa taacataagc
    24361 ttattaaatg atatctttat caataacgtt attgttggat tgcagtatag tcttgaccat
    24421 gtaacatcca acgaacaccg tatttatcgg taaagatgcc cattttgcca ccccagaatt
    24481 ggtcagcaaa cggtaattct atttcaattg aagaatgatc tttaatttgc tcatagaatg
    24541 cttcaacttt atcagcatct tccttattgt taacatcata atcaattaat aatgatatgc
    24601 cattattaat tttgtcagcg cgaccaaaag aatcagaaca tagcactttt acaccaagca
    24661 cttcaaactc agcatgcata gttgcttctt gtgcatcttc cttagtcata ccaaaatgtg
    24721 acgcttgttc ttcgccaact tctaaacgtt taacgtcagt tgcaccaaat acttcttcgt
    24781 aatatgcaag ggcttctttt gaattttcaa aagcgatata agggaataat gctgtcatgt
    24841 aaaacacctc aatgtattat ttttattact aacactatat tacattaaaa aatggcaaaa
    24901 gtataacttt tacgatgaat tgaagtcaat cttttgtaaa atacacgata aattgtaaga
    24961 tgaaattgat ttaaatgaag taaacatata tatatgacat acaaaaaatg aaaatgacac
    25021 agagacgtat gtgttgttta gtgttttaaa attcaagttt aaaaaatgct tttagataca
    25081 taagggattt tcaaaatata aatgcattaa aatgaatttc
//
LOCUS       DAKSQE010000027        24553 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.027, whole genome shotgun sequence.
ACCESSION   DAKSQE010000027 DAKSQE010000000
VERSION     DAKSQE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24553
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.027"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(404..508)
                     /locus_tag="O0V33_000967"
     CDS             complement(404..508)
                     /locus_tag="O0V33_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975623.1"
                     /translation="MLLLERTSMSDFEMLMIVLTIIGLVLISTQDHKK"
     gene            1188..1346
                     /locus_tag="O0V33_000968"
     CDS             1188..1346
                     /locus_tag="O0V33_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCX8975624.1"
                     /translation="MSKSSDALFNSLTQSFELIEGVYNELVTDNLKIVMYKPRKESTS
                     GRINRRFK"
     gene            complement(2005..2862)
                     /locus_tag="O0V33_000969"
     CDS             complement(2005..2862)
                     /locus_tag="O0V33_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCX8975625.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASTYTKDVINRLREKGYKVFLAT
                     GRSHSEIHQLVPEDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQIINLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     EHFGINQKETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAKHFLAD"
     gene            complement(2930..3712)
                     /locus_tag="O0V33_000970"
     CDS             complement(2930..3712)
                     /locus_tag="O0V33_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8975626.1"
                     /translation="MLIQLDQVGRMKQGKTILKNISWQINEGDKWILYGLNGAGKTTL
                     LNILNAYEPATTGYVNLFGKMPGKVGYSTETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDDEVRNEAHQLLKLVGMSAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLSILDSLSDSYPTLAMIYVTHFIEEITGNFTKILLLK
                     DGESVQQGLIDDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            complement(3944..5749)
                     /gene="glmS"
                     /locus_tag="O0V33_000971"
     CDS             complement(3944..5749)
                     /gene="glmS"
                     /locus_tag="O0V33_000971"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCX8975627.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     EGTPVVGLATQEKVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVTLQLISYYAALHRDLDVDKPRNLAKSVTVE"
     gene            6430..7968
                     /locus_tag="O0V33_000972"
     CDS             6430..7968
                     /locus_tag="O0V33_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCX8975628.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWFPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFFGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLATLVSFV
                     VAALIMKFTKEPKQDLEAATAQMETTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SLDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            8003..10135
                     /locus_tag="O0V33_000973"
     CDS             8003..10135
                     /locus_tag="O0V33_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCX8975629.1"
                     /translation="MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRITGADSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNLAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVKKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLEQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETRDKSSNMI
                     DKDDVHIETKDIDGNTSFENEQTSYLTSVFEKHLSDEKSEQLMHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFIPDNDFMASLISDLSEFLSLKLES
                     IDTFMENPQELETLLRNKFLERIKKQFI"
     gene            10147..10581
                     /locus_tag="O0V33_000974"
     CDS             10147..10581
                     /locus_tag="O0V33_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCX8975630.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEGNVDRIVQAKSAEEIKQVFEEADA"
     gene            10581..11687
                     /locus_tag="O0V33_000975"
     CDS             10581..11687
                     /locus_tag="O0V33_000975"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCX8975631.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALATGH
                     QYDVILADESKTTTRVNNVAAINSMQPSEELKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVKDSSIEAGLRRVLAETSQYITNEFDFTEAEQAGYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLAYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(12006..19394)
                     /locus_tag="O0V33_000976"
                     /pseudo
     CDS             complement(12006..19394)
                     /locus_tag="O0V33_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
     gene            complement(19677..21032)
                     /locus_tag="O0V33_000977"
     CDS             complement(19677..21032)
                     /locus_tag="O0V33_000977"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCX8975632.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIVLDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            complement(21059..21991)
                     /locus_tag="O0V33_000978"
     CDS             complement(21059..21991)
                     /locus_tag="O0V33_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HCX8975633.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKITGGEEQLNDIAYLKATFKTNKKINGDTKDIAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVSKAEPSETKAYINVK"
     gene            complement(21993..22802)
                     /locus_tag="O0V33_000979"
     CDS             complement(21993..22802)
                     /locus_tag="O0V33_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCX8975634.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
     gene            complement(22992..23900)
                     /gene="rocF"
                     /locus_tag="O0V33_000980"
     CDS             complement(22992..23900)
                     /gene="rocF"
                     /locus_tag="O0V33_000980"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HCX8975635.1"
                     /translation="MTKKKGIDIIGVPSTFGQRKLGVDLGPTAIRYAGLIPRLKQLDL
                     DVNDKGDIKVSAVDIEKFQSEQNGLRNYDEIIDVTQKLNKEVSASIENNRFPLVLGGD
                     HSIAIGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLTYRESHFALELLHQSQSVTSMDLVEVN
                     PLIDHNNHTAEQSASLVGTFFGETLL"
     gene            complement(24042..24114)
                     /locus_tag="O0V33_000981"
     tRNA            complement(24042..24114)
                     /locus_tag="O0V33_000981"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24079..24081),aa:Lys,seq:ttt)
     gene            complement(24118..24192)
                     /locus_tag="O0V33_000982"
     tRNA            complement(24118..24192)
                     /locus_tag="O0V33_000982"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24158..24160),aa:Gln,seq:ttg)
     gene            complement(24202..24285)
                     /locus_tag="O0V33_000983"
     tRNA            complement(24202..24285)
                     /locus_tag="O0V33_000983"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24249..24251),aa:Tyr,seq:gta)
     gene            complement(24314..24389)
                     /locus_tag="O0V33_000984"
     tRNA            complement(24314..24389)
                     /locus_tag="O0V33_000984"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24354..24356),aa:Val,seq:tac)
     gene            complement(24397..24468)
                     /locus_tag="O0V33_000985"
     tRNA            complement(24397..24468)
                     /locus_tag="O0V33_000985"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24433..24435),aa:Glu,seq:ttc)
     gene            complement(24470..24544)
                     /locus_tag="O0V33_000986"
     tRNA            complement(24470..24544)
                     /locus_tag="O0V33_000986"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24510..24512),aa:Asn,seq:gtt)
BASE COUNT         7163 a         4440 c         3931 g         9019 t
ORIGIN      
        1 ttatgcagtt gatgcgcagc atccaactgt gttcctctat ttttcaactt tagttgttag
       61 gggcttcttt ctaaaataac taagtgaatt atctatatat gaagaggtct ccaatacttt
      121 ttatataatt cgtgattaca ttctatactt ttaattgtat tttctgctgt atggaaagtt
      181 ggttttatta attctgatta tgttcagaac tacaattcat aagaaaaatg tgttaaaata
      241 tatttgtagt aagtagaagc aaaagatgaa aatctttaac tcttgaaaca caaaaagggc
      301 aacactcgga aacatgttac cctaatgagc ccgttaaaaa gacggtgacc ttatatttta
      361 tttaaaaata gccttcaaaa tgccggtcaa agcgaataga aggttatttt ttatggtctt
      421 gagtactaat caatactaaa ccaatgattg ttaatacaat catcagcatt tcaaaatcag
      481 acatgctagt cctttctagg agtaacaact ataaccatag gcatcacccc tttattaaga
      541 gattagccac catctatcca acttgctcaa taaatattat aacatgcaca cttttaaaca
      601 gtaattcata gtataagttt tttcatttct taatcaactc gataaacagt tgagtgacat
      661 acccttgtct aactagctat ttcttcccct gttatcgttt gttcatcata taaaaattta
      721 atttctcatt ttttattctc tgataaagaa gagcaccaac cttttcggct gatatgctcc
      781 cttcaaaata gacattgaaa aatgaaaact ttgaaagaga aatcgtcagt ctattattga
      841 taagtattat cctatcctcg aaaaattact ttcagacaca agtgaacaaa aattttacta
      901 taagcgtata ctttggcaat attcgaagga aaaacatgga ttaacctatg catattatac
      961 atttagagct tatatactta aacatgatga atttactcct tatattatga aatgttatca
     1021 atagatgtcg cctaaagata caacaagatt cgagacaaaa cctgatcatc aagcacagtt
     1081 tgactggaaa gaaagcatta actttaaaac gaaagataat caaaggattc cactatatat
     1141 aggtgtgcta ctactttatt attctcgttt tataatcatg tatgacaatg agtaaatcaa
     1201 gtgatgcctt atttaattca ttaacacaat cgtttgagct aatagaaggt gtatataatg
     1261 aacttgtaac ggataacttg aaaatagtca tgtataagcc aagaaaagaa agtacaagtg
     1321 gtcgaataaa tcgtagattt aaataattcg ctgatggttt taaatttcaa gtaaaacctt
     1381 gtatttccgg cctccctaga aaaagataaa gttgaatcaa taacaaaaat attagatgaa
     1441 attaatgctt accaagacga attgcatttg gaagagattc ctaagttttt ttaatcttaa
     1501 tgaacgattt aactactaag ttcatactag tacaggtaaa ttgcctatta ctgcctaaga
     1561 gaaagaaaaa attttcttac aaccactaca gaatgtgaat gtaaaaaact catataaagt
     1621 gaaacatatc ttacttatgg aatgaaatgt ttgattatcc caaaatcaca aatgcgattt
     1681 tggagttgtt aggggctcta acgcaaatac actcctactt aaaataacgg tagttgttat
     1741 agtatactta aatgcataaa ccatataaaa ggtgatgcta atatgacata cggttcaatt
     1801 gtagctatac tctgtgccct atttgtgatt ttcttcattc catatatgga gaagaaagac
     1861 aaaaagaaga aataacacta gtatctggat tactagcata caaaggagca gtaagacatt
     1921 ttctaataga aaataacttt actgctcctt tgtaaattac tcttcaacta ttgtaaatta
     1981 aataataaat aactgccgat gttattaatc agctaaaaaa tgctttgcta aatattttgc
     2041 tgcgccatcc tcttcattcg tataagccgt tacatctgaa gttaatgctt ggatttcagg
     2101 acgtgcattt ttcatagcga ctgtgtaatg gccaaattca aacattgctc tatcattgtc
     2161 gctatctcca ataactaacg tttctttttg attgatgcca aaatgttcaa tcatttcttt
     2221 aatgcctgta cctttatcag tttgataagc cattgtttcc gcattaaatc ttgatgaatt
     2281 tgaaacacta atctgtagtt gcacatgatt ttgctttaat tcatctctaa atgctgttat
     2341 tttttctaaa ttagaactga acagataaat ttttgaatat tcaccttcag gaaacttagt
     2401 tacccaatct atcttaccag caagcgcatc ttgtcttgaa gaccattcac tatgacttac
     2461 gccattaata ggatcttgac tacgaatcat atctcgcatc catgtttcat cttcttttaa
     2521 agaaactcta ttaccttcaa aaggaaatac ctcataataa atttgttggc gcttagctaa
     2581 attaataatt tgttgcactt gagccaatga taaaccatgt ttgaagataa tttctccatc
     2641 tacttctcca atggttccat ttgaactaat gatgccattt accgcaaaat cttcaggtac
     2701 aagttgatgt atttcagaat gcgaacgtcc agtcgctaaa aatactttat atcctttctc
     2761 tctcaatcga ttaatgacat ctttcgtata cgttgatgct tgattatttt catgtaaaat
     2821 tgtaccatcc atgtccaaaa atattgcttt tacgttatcc atattgccct ccactgtcaa
     2881 ttttagctta attaaatttt gtcattatta gttagtattt gcaaaatatt tactctaaca
     2941 tcgccataga aaatcgatta ttccatcttt gaactgctac atttttctgg aaaaatcgtg
     3001 acatgttttc agatgtcaaa atatcatcta ttaacccttg ttgtacactt tccccatctt
     3061 ttaatagcaa aattttagta aagttaccag taatttcttc aataaagtgc gtcacataaa
     3121 tcatcgcaag cgttggatat gaatctgaca atgagtcaag tatacttaac aacgattctc
     3181 gtgcaataaa gtctaaacca gctgctggct catctaaaat taaaacctgg ggttgcccca
     3241 ttaaagctcg cgcaatcatc actcgttgtt tttcaccggt agataaataa ccaatatatt
     3301 gttgtgcttt agcagacatt ccaactaatt taagtaattg atgtgcttca ttacgtacct
     3361 catcatcaat atcttgataa acaccaattg atttaaaagc accgcttatc actacatcga
     3421 ttactctttc accctcttga aacttttcca gtaaactatg agatacaaaa cctatatgtt
     3481 gtcgtacagt ttctgtagaa taccctacct tgccgggcat tttaccaaat agattaacat
     3541 agccagttgt cgcaggctca tacgcattta aaatatttag aagtgttgtc ttgccagcac
     3601 catttaaacc atatagaatc catttatcac cttcattaat ttgccaagaa atatttttta
     3661 aaattgtttt tccttgcttc attcgcccaa cttgatctaa ttgaattaac atgcgatgcc
     3721 acccctttat tgctactcca tacttctcat aaaatcatat taacatatca ctcttaattg
     3781 tcagactatt taaataaata aactacttca tttttacgca tttctgccga attaagatga
     3841 agtaaacgct aagtcgacct aaaaaagcac ccttttagtc gattaatcta aaagggtgcc
     3901 atatacttta attttaatac atgattgatt ctaaaaaagt gaattattcc acagtaactg
     3961 atttagcaag gttacgtggt ttatcaacat ctaaatctct gtgtaatgct gcatagtatg
     4021 aaattaattg taatgtaaca actgatacta atggcgttaa taattcatgt acatgaggaa
     4081 tgacataagt gtcgccttct ttttcaaggc cctccataga aataatacat gggtgtgctc
     4141 cacgtgctac tacctcttta acattaccac gaattgataa attaactttc tcttgtgttg
     4201 ctaaacctac aactggtgta ccttcttcga ttaaggcaat tgtaccgtgt ttaagttctc
     4261 cacctgcaaa accttcagct tgaatgtaag agatttcttt aagttttaac gcaccttcta
     4321 aacttacgtt atagtcaata gtacgtccga taaagaatgc attgcgtgtt gtttctaaga
     4381 aatctgtagc aatttgttcc atgattggtg catcgtcaac aatcgcttca attgctgttg
     4441 ttacttttgc taattctctc aataaatcaa tatctgcttc acgaccgtgc tcttttgcaa
     4501 cgatttgaga caagattgat aatactgcaa tttgtgcagt atatgctttt gtagatgcaa
     4561 ctgcgatttc aggacccgcg tgtaataaca atgtgtggtc tgcttcacgt gataaagttg
     4621 aacctgcaac attagtgatt gttaatgatt tatgacctaa tttattagtt tcaactaata
     4681 cggcgcggct atctgcagtt tcacctgatt gagaaatata aacgaacaaa ggtttttcag
     4741 ataataatgg catgttgtag acaaactcag atgcaacgtg tacttcagtt ggtacgccag
     4801 cccatttttc taaaaattct ttacctacta aacctgcatg gtagcttgta cctgctgcaa
     4861 taacgtaaat gcggtctgct tctttaacat cattaatgat gtcttgatca attttcaagt
     4921 taccttctgc atcttgatat tcttgaataa tacgacgcat tactgctggt tgttcatgaa
     4981 tttcttttaa catgtagtgt gcataaacac ctttttcagc atctgatgca tcaatttcag
     5041 caatatatga atcacgttct acaacgtttc catctgcatc tttaataata acttcatctt
     5101 ttttaacaat aacgatttca tggtcatgga tttctttata ttcgcttgtc acttgtaaca
     5161 ttgcaagtgc gtctgatgcg ataacattga aaccttcacc aacacctaat aataatggtg
     5221 atttattttt agcaacatag attgtgtctt tgtcttcagc atctaataaa cctaatgcat
     5281 atgaaccatg taataatgac acaacttttg taaatgcttc ctcagttgaa agtccttgat
     5341 ttgaaaagta ttcaactaat tgaacgataa cttctgtatc tgtttctgaa atgaatgata
     5401 caccttgtaa gtattcacct tttaactctt catagttttc aataacaccg ttatgaacta
     5461 gagtaaaacg gccatttgat gattgatgtg gatgagagtt ttcatgattc ggtacaccgt
     5521 gtgttgccca acgtgtgtga ccgattccaa caggaccatc aaaatcgcta ctatcagcaa
     5581 ctttacgtaa ttctgcaata cgaccttttt ctttgaatac agttgtatta tcatcattta
     5641 ctactgcgat acctgcagag tcataacctc tgtattctaa tttttctaaa ccttttaata
     5701 ataattcttt ggcattatca tagccaatat aaccaacaat tccacacata acgacatttt
     5761 cctccatatt ggaatagtac gcgtaaatta tgatttattg ccgataattt agattgacaa
     5821 tctgctttca taatataaat aggaacatgc tatcatcgca ttcatccata acaaattaag
     5881 catagttatt tttacaacta tgcaaattgc tcacactgta ctttccatat taatattttt
     5941 tatattcaat ttctggcgat cttattaact ttgtccatta agtcacccta atgttttact
     6001 taataagcta acgaatgagc cacatccggg atagcatccg ccgatctatt cgatcactat
     6061 cctcctcgtc tacaaataca tattttgcac tctataaagg ccactcatat attaaccttt
     6121 aatcttcaaa tacaaatatt tatttgcaca ggcgctttaa ctgtactgcc gaactttccc
     6181 cctttccatt aatcattatt gtacaacggt gttgttttgt tttgcaaata ttttcacaat
     6241 aaaattttaa aaatcctaaa acaatttttt tgttttactt tttcaaaata tctacgcagt
     6301 cacattgatg acactttatt taattttgtc acatttattt tgacaaagtt gatttttgtt
     6361 tatattgagg attgcgttat ggctctttac actaaagata gtcacataca ttaaaaaaga
     6421 ggtgtaaata tgtcgcaaac tgaagagaaa aaaggaattg gtcgtcgcgt tcaagcattc
     6481 ggatcatttt tgagtagtat gattatgcca aacattggtg cattcatcgc ttggggtttc
     6541 atcgcggcca tttttataga taatggttgg ttcccgaata aagatttagc tacgttggct
     6601 ggtccaatga ttacttattt aatcccacta ttgattgcat ttagtggcgg tcgtttaatt
     6661 tatgatttac gtggtggtat tatcgctgca acggcaacta tgggggtcat cgttgcatta
     6721 cctgatacac caatgttact tggagctatg attatgggtc ctttagttgg ttggttaatg
     6781 aaaaagactg accaattgat ccaacctaga acaccacaag gttttgaaat gctatttaat
     6841 aatttttcag ctggtatttt aggatttatc atgacaattg caggatttaa aattttagca
     6901 ccacttatga aatttatcat gcatatttta tcagtggcag ttgaagcatt agtacatgca
     6961 cacctacttc cacttgtaag tattttagta gagcctgcaa aaattgtatt tttaaacaat
     7021 gcgattaacc atggtgtatt cacaccgctt ggtgcagatc aagctgcaaa agctggtcaa
     7081 tctattttat acacaattga atctaaccct ggaccaggtt taggaatttt acttgcatat
     7141 atgatttttg ggaaaggtac tgcaaaagca acttcatatg gtgctggaat tatccacttc
     7201 ttcggtggta ttcatgaaat ttatttccca tatgtattaa tgcgtccttt attattcatc
     7261 gctgttattt taggcggaat gactggtgtt gcaacttatc aagcaacagg atttggattt
     7321 aaaagtcccg cgtcacctgg ttcatttata gtttactgtt taaatgcacc tagaggtgaa
     7381 ttcttgcaca tgttactcgg tgtcttcctt gctacacttg tatcatttgt cgtagcagct
     7441 ttaattatga agttcactaa agaacctaaa caagatttag aagctgcaac tgctcaaatg
     7501 gaaactacta aaggtaaaaa atctagtgtt gcatctaagt tagtatcttc tgataaaaat
     7561 gttaatacag aagaaaatgc tagtggtaat gttagtgaaa catcttcatt agatgatgat
     7621 cctgaagcgc tattggataa ttacaacact gaagatgttg atgcacacaa ttacaataat
     7681 ataaatcatg ttatttttgc ctgcgatgcg ggtatgggtt ctagtgcaat gggtgcaagc
     7741 atgttacgta ataaatttaa aaaagcgggc attaatgata ttacagttac aaatactgca
     7801 attaatcaat tgccaaaaga tgctcaatta gttatcactc aaaaaaaact aactgatcgt
     7861 gcaattaaac aaacaccaaa tgccatccat atttcagtgg ataatttcct taattcacca
     7921 agatatgaag aacttttaaa taatctaaaa aaagatgatc aagcataata attaaataaa
     7981 ttaaaaaatg gaggataccg ccatgttatt gagtacacgt gaaaaagaaa tgatagccct
     8041 attgattaag taccacggtc aatatatcac tatacacgac attgctcagc aacttgcggt
     8101 gtcctctcgt actattcacc gtgaattaaa aggtgttgaa gcatatttaa cttcattttc
     8161 attaacttta gaacgcgcaa acaaaaaagg gctacgcatt actggcgcag attctgattt
     8221 aaacgatttg aagcaatcga ttgcacaaca tcaaaccatt gacttatctg ttgaagagca
     8281 gaaagtaatt attatatacg ctttgataca agccaaggag ccagttaaac aatatagttt
     8341 agcgcaagaa attggcgttt ctgtccagac tttagcaaag atgttagatg atttagagct
     8401 cgatttaaat aagtaccaac tatctttatc tcgaaagcgt ggcgaaggta tttacttggt
     8461 aggtactgaa tcaaagaaac gtgaattttt aagtcaatta atggtgaata acttaaatag
     8521 tactagcgtt tattcagtaa ttgaaaatca ttttgtcttt cattcattaa atcaaatcca
     8581 caaagacttt gttgacttag agcgtatttt taatgttgaa agactattaa tggactacct
     8641 aagtgcctta ccctaccaac ttaccgaatc aagttattta actttaactg tccatatcgt
     8701 gctatccatt tcacgtataa aaaatggaga gtatgtcgca ttaaacgatg atatttatga
     8761 ttctgtacaa aacacatttg aacacaaagt agcaagcgaa cttgctgata aacttggtca
     8821 aatatatgac gtcacgttta atctggcaga aattgctttc attactatcc atttacgtgg
     8881 tgctaaacga aaaaatctta atgatacatc attaaataat cgttgtgaag aaaacaaaat
     8941 taaagcgttt gttaaaaaag tagaaatgat ttcaggtatg acatttgcag atttggatac
     9001 tttagtagat ggactgacgc tacaccttaa tcctgcaatc aatcgtttgc aagctaatat
     9061 cgagacctat aatccgttaa cagacatgat taagttcaaa tatccaagac tgtttgaaaa
     9121 tgtaagatta gctttaaatg attgttggcc tgatttgatt tttccagaga gtgaaattgc
     9181 ttttatagtt ttacactttg gtggctcgat taaaaaccaa ggtaatcgat ttttaaacat
     9241 attagtcgtt tgcagcagtg gtatgggaac tagccgtcta ttatcaactc gtctagaaca
     9301 agtttttagt gagattgagc gtattacaca agcatcagtc agcgatttga agtcactaga
     9361 tttaagtcaa tatgatggca ttatttctac tgtgaattta gacatagact ccccctattt
     9421 aacggtaaac ccattattac cagatagtga tatcagttat gtcgcacagt ttttaaatac
     9481 aaagtctacg ttccaagaga cgcgcgataa atcatcaaac atgattgata aggatgatgt
     9541 tcatattgaa acgaaagata ttgatggcaa cacatctttt gaaaatgaac aaacttcata
     9601 cttaacttca gttttcgaaa aacatttaag tgacgaaaaa tcagaacaat taatgcatca
     9661 tatgcgttcg ggtttaactt tgcttgattc agtgaaaata gttagtaccg aagttaaaca
     9721 gtggcaaaca tatatcgcag attatctata tcaatgcgat gtaataaacg atccaacgtc
     9781 attcgctgaa ctactagagc aacgattgat tgacaatcca ggatggatat taagtccata
     9841 tcctgttgca ataccacacc taagagacaa tatgattaaa caccctatga ttctaatcac
     9901 agttttagaa gaaccgttaa cattgcctag tattcaaaat gacaatcaaa caattaaata
     9961 tatgatttcc atgtttattc ctgacaatga ttttatggca tcactgataa gtgacttgtc
    10021 cgaattttta agtttgaaat tagaatctat tgatactttt atggaaaatc cacaggaact
    10081 tgaaacatta ttaagaaaca aatttttaga acgaattaaa aaacaattta tttaggagtg
    10141 tacaaaatga gcgaattatt tagtaatgac aatatctttt taaatgtaaa tgttaacagc
    10201 caaaatgaag caattgaaaa agcaggtaaa gccttagttg atagtggtgc tgtaacagat
    10261 gcttatattc aagcaatgaa agatcgtgag caagtcgtat caacatttat gggaaatggc
    10321 ttagcaattc ctcatggcac agatgaagct aaaacaaatg tgattcactc aggtttaaca
    10381 ttattacaaa tccctgaagg cgttgactgg gatggcgaag tagttaaagt tgtcgtggga
    10441 atcgctggta aagatggcga acatttagac ttgttatcta aaattgcaat tacatttagc
    10501 gaagaaggaa atgtagaccg tatcgttcaa gcaaaatctg cagaagaaat taaacaagtt
    10561 ttcgaggagg cagatgcata atgaaagcag ttcattttgg tgctggtaat ataggtcgtg
    10621 gatttattgg atatattcta gcagataaca atgtaaaagt aacatttgca gatgtaaatg
    10681 aagaaatcat taatgcatta gctactggac atcaatatga tgttatttta gctgatgagt
    10741 ctaaaacaac aacacgtgta aataatgttg cagcgattaa ctcaatgcaa ccttctgaag
    10801 aattgaaaca agcaatttta gaagcagata ttattacaac agctgttggt gttaatatac
    10861 tacctattat tgctaaatct tttgcgcctt tcttaaaaga aaaaacaaac catgttaata
    10921 ttgttgcttg tgagaatgct attatggcaa ctgatacatt gaaaaaagcg gtacttgata
    10981 ttactggacc tcttggtaac aatattcatt ttgctaactc agcagttgat agaattgtac
    11041 cattacaaaa gaatgaaaat atattagacg ttatggttga gccattttat gaatgggttg
    11101 ttgaaaaaga tgcatggtat ggtccagaac taaaccatat taaatatgtt gatgatttaa
    11161 caccatatat tgagcgtaaa ttattaactg tgaatacagg acatgcatat ttagcgtatg
    11221 ctggaaaatt tgctggtaaa gctacagttt tagacgctgt aaaagatagt tcaattgaag
    11281 ctggcttacg ccgtgtttta gctgaaacaa gtcaatatat tactaatgaa tttgatttta
    11341 ctgaagcgga acaagctggt tatgttgaaa aaataataga ccgtttcaac aattcttatt
    11401 tatctgatga agtaacacgt gtcggacgag gtacgttacg taaaattggc cctaaagata
    11461 gaattataaa accattagca tatctttata ataaagattt agaacgcact ggtttattaa
    11521 atacagctgc attgttattg aagtatgatg atacagcaga ccaagaaact gttgagaaaa
    11581 ataattacat taaagaacac ggtttaaaag cgttcttaag cgaatatgct aaagttgacg
    11641 atggcttagc cgatgaaata attgaagctt acaattcact ttcataattt attgagcttt
    11701 gtttgaaact ataattttcc aacgttattc gttaacaatc agtaataatg tagtagttcc
    11761 gttgaattaa caatattaca tttctgaaca taaaaaatac tcccttcaac atagacactt
    11821 aacttgtgtt atgtatgaaa ggagtatttt tgcgttaata atttgtttta ttttcgagcc
    11881 acagccacct attcaatggc tattggtcat tactaaaaca aattcatatt aactgttaga
    11941 cttggttact tagtaaggaa tatttcccta tgaaataact agatgttcac attcttaaat
    12001 aaattttatt cttcagtttg ttggtctttc ttagtgaatc ttctaattaa gaatgccata
    12061 cctgcaccta gagctaattc aacatatggt aaatcgtcat tatgtgacat accagtatct
    12121 ggtaaagttt tagcttgttg ttttgcttta ttttctttac cttgttgcgc tgatgttgcc
    12181 ttagcttggt gtttgtcagt gttagttact ttaagcatat cttgattagc actattgctt
    12241 ccatttgaaa ctgtagctgg agatgcattg gcaccgtcgt tttgcgtagc tttattgttt
    12301 acatctgcag ttgcaccaat tgctgcttga ttatcttttt tatctgatgt tgccatgtca
    12361 gccttttgac tatcctcagt cgaagtcgct ttatcttttg cttctgacgg tttagatgtt
    12421 gatggtttat tcgatacatc agtatcaacg ttgccttcag atttatctgc ttcgttagat
    12481 gcaacgttag tttcagactt aagttgtcca gcatctgttt gatttgtcgt actttctttt
    12541 ttatctttct ctgtattaga aggtactttt ggttctgtaa tgtctgctga aggcaatgtt
    12601 tcagttgttg attcaaccat actttgattt gttgaatctc taccatcttt ttcatcctta
    12661 gctttatttt cagattttgg ttgtgcaacc ttgccattag ttgattgaga ttcagcacta
    12721 ttatttactt cagcattttg ttttgaatca tttacagatg cattatcttt gctatcagca
    12781 gatgatgctg cacctgtgct cgcatttgtt gttggaaccg ttgctgttga tcctgttggt
    12841 gcgttctcgt ttgttgcagt tgtactattg ttatttcttg cgctttctgc tggcgttgca
    12901 ttatctgttt ctgttgcaga tttatcagtt gtgccgttat tagttgattc tacttctgat
    12961 ttatcacttg taccattacc cgttgccgaa ttgtctgttg atgttgctgt tgttccacca
    13021 gttgccgcat cattatgatt atttgtcgtt gttgaagacg tatctgcatt ggcattatct
    13081 gttgactcag ttacattagg ttgtccagta ttttctggtg ttgcattagc atttgaattt
    13141 gctgttgcat cattattatc tgaaccacta ttagtatcat tagcatttgg atccttctga
    13201 ggtacaatcg cttcaattgc aggtatcgtt acattttgta attcatcaac ttctgtattt
    13261 gtttgcgttt tatctaattt atcagcaaat ctgtcaaaat atctacctaa atctgtacgt
    13321 gcaatttctt tcgccgatgc atctgcattt tcaattattt ctatttgctt gttaaccact
    13381 tctctaattg cttccaaagc atttttctta actactggat taatacgttg tgctttaagt
    13441 tgttcaagcg cactattttt gacagtagcg atttctgcat ttgtagtttg atcagaaata
    13501 tcttcagttg cttttgataa aatgtcttct aatgcattcg taaacgcttc tttttcttca
    13561 gttgtagcat cagcgtcgac atttactcct gcttcaatct ggtctagtgc agtttctaat
    13621 tcttcgatag ctttttgttt ttctgttgag tcaatttgaa tgttatcaaa cgcctcaagt
    13681 ccttgagctt tcgctttttc aacttcagca gttgttgttg catcagtaat accttgttta
    13741 gcttgatctg taatttgttt aatcgttgct aatgcagccg ttttttcttc agcagttaac
    13801 tggtcacttt ggtctataga ctctttcgtt tcctgtgctt tagcatcaat cgcttggttt
    13861 gctttaggtt taacagtagc atctacttga actgcatcaa ttgtcgcttt accttgtgtt
    13921 aatgcatcat caacatcact attatccaca ccattattaa tgctttctaa tgcagtttga
    13981 acattttggt caacttgctt aattgcttgt tgtttttcat cttgtgttgc attagtgtca
    14041 gctaaaatat tatttttctt ctgatctgca taggcatata aatctgttgt agccgattct
    14101 tttttaccgg ttggaattgt gtaatcgtta atattatcta agtcattatg aatttgaact
    14161 tcaatgtcat ctttagaagt agcttgatta acattttgat ccgctgtttg ttttaattca
    14221 gcaagttttt gtttcgcttc agcaatttca cttgaagtcg atgcgttaga gttatccgct
    14281 tcgttcactt tagcgttata tgcatctgca attttagcta aagcatcttt tttgtactca
    14341 ctaaatgttt taactgcatt aattttagct ttcccttctt taaccgcatt atcaacatat
    14401 tcatttgttg atgattgatc aatatttgtt ttcgcttgat ttaacgctgc atcaacttct
    14461 tgttttgcat cattaatttc ttgttgtgac gcatttggtg tttgttcaat ttgtgttttc
    14521 ttatcagctg cagcttgatc taattctgtt ttacctgctg gtttcttaac aggatttgca
    14581 tgaagttgtt gaactttttg tactgctgta tctttagcag tagttacatc acctgtagta
    14641 gttgctgcat taatattatt taaaccttct tcatatgctg ctctaactgg ttctatatct
    14701 tttccttttt cttcatttgt agtcgtatta tcattaagga tttcagttat tttattttgc
    14761 atttcattta gcaattccgc tcttgcattc gttttaacat ctgttgatgc ttgaattggg
    14821 tcaattgctt gaattgcatt atcttttgca gtgtttacat catcgattga ctttgcattt
    14881 tcaatatttt gattaccttg cgttaattgt gcatctactt gttgatttgc ttgttctttt
    14941 tcttcagtag tcgcatctgc agtttgtgca ataagtgctt tttgttcctt tgcttttgtt
    15001 gctaattcat ctttagctac atctttaatt gtcgtatcag cagtaatacc ttgaatgtca
    15061 gcaactgctt gatctttaat ttgtgtaaca tcgttagtcg tttgcgcatt taaaatatct
    15121 tgatatgctt tttctttagc tttagaaact aaatcttttg ctgcattttt ctcttcagtc
    15181 gttgcacctg tagtattatc aattgcttga ttttgagttg tcacagcttg atctacttca
    15241 tttttcgcat tcgatttaac tgctgttgct ggttgtgtac tttgaatcga atttttacct
    15301 gcatctttag cagtgtctac accattattg tcagttgcag ctgtaatgtt atttttcgcg
    15361 tctgtaactg ctgttgctag ttgttgaatc gccgcttctt tttcttcatt tgttgcgttt
    15421 tgatcgttat taataaccgc tgtttgtgct gcttgtaatt gatcgatctc atctttcgct
    15481 gcttgtttct tcataacttt tggtgttaca gcgttaattg ccgcttctgc attagttttt
    15541 gcctcatcta cttctgcatt tgttgtagct gctgtgatgg cttgatttgc tttagcattt
    15601 tcagtattag cttcagtttc tgctgcttgt ttctcttcat ctgttgcatc tggcgtagct
    15661 tgaattgctt gtaacttgtt atttaatatt gtagtgattt cattacgtgc tgtagctttt
    15721 ttattaacag ttggtgtcac ttgatcaata ctagcttcac ctgttgtctt cgcttgatca
    15781 acttcatttt gactattagc agtctcaatg tggttgtttg cttctgttac tgcattatct
    15841 acgttcgcat tagccgctgc aatttcttct gctgtaatct cttgcgtttg agcaatggct
    15901 gctttacgtt cattcgcttt cgtagcaatg gcttgtttcg catcatcttt cgttgttgtc
    15961 gctggttgaa ttgcttcaat tttagcaatt tctgcattct tagccgcttc aacttctgca
    16021 tttgaatgtg caccatctat tgctgtatca gctgtcgttt tttctgtttg aacttgttgt
    16081 ttcgctgctg ttttctcttc tgttgttgca ccgttattag catcaattgc tgtttcttgc
    16141 gcttgtactt tatctgcaat tgcttgtttc gcttgaggtt taacatttgc atcaggtgta
    16201 atggctgcga ttgtagcttc atttgtagtt tttgcattat ccacatcatt gtttgctgcg
    16261 gcattatcaa tatcagcgtt tgcattaact actgcttgat ccactttatc tttcgctgct
    16321 tgttgttctt cagtagtcga atcattcatt gcttcgattg cagttttacg ttcacttgct
    16381 ttttgagcga tttctgcttt agcatccgat ttcttagtag tggcagcttg aacttgatta
    16441 attgcagcaa taccattgtc tttagctgtc gttacagcac tgtttgtatt tgcagcatca
    16501 agattcgttc ttgcctcttg tttttttgca tctaattgcg tatatgccgc ttctttttct
    16561 tctgttgttg aagtgttact attttggatt tcttgtttac gtgtattata tgcgttgtct
    16621 acttctctat ccgctgcagg tttaactcta gcttgtgttt gaatggcatt gattttatct
    16681 aatacttttg ctttagtatc agccacttcc tgttttgatt taacaacttg gatgtcatgt
    16741 aaaccttctg tttgagctgc atctactgct gcatcagctt ctgctacttc ttcatcagtt
    16801 gcatttgaaa ctgtagcatt ttgagctttt ctagctgtag cagcttgttt aacttcatta
    16861 taggcatcca tttttgtttg cgtagagtcc gtaatattct caattttgaa tatttcttcg
    16921 tttttaactc tatctaaatc ttgtgcagtc gttgtcgctt caattgcttt aacacctgaa
    16981 actttagctg cattaatacg ttcaatcgct tccgctacct cttcatttgt tgtatcagga
    17041 tttaaaggtg cttgatcaat ttgtgcttga acaacttcgt caaattcttc aagagctgct
    17101 gctttagctg ttgtagcagg tgcagtttga ttaatatcat tgatcgcttt tgttttaatg
    17161 gcttctactt gtgcatttgt tgttgctgca tcaatatctt taattgcctt tgtttcaatt
    17221 ttaccaattt tatcatttgc tacatctttt tcatcttgta atgatgcatt tgacttttta
    17281 atttgttgtt tacgagttgt aactgcttgg ataatatctt gtttcgcttg aggtttaact
    17341 gttggtgtaa tcacatcttg ttccaacact gcgataccat tatctttttc agttgtgaca
    17401 ccttgtgctg ttgtttgttt attaatttct gcgattgcat tatttttatc attgttaatt
    17461 ttagtcacaa gcgctgcaac ttcatcttgc gtaacttttt tactttgttg tgctgctgta
    17521 accttttctt gagctttagc atctaattca gaaattgctg cttgattatc aattaatgca
    17581 gcttgtaatt tagttactaa accatcaata tccgcttgag atgcacgatt tgcagtttca
    17641 acatgattcg catcttcatt taaaatagtg tctgcttgtt gtttaagttt attgtattct
    17701 gcaattgatg ctgttgtgta atgactatca tcaacttgtg agttaacttg ttgttgcaac
    17761 gcatctttgt tcatttcaac tgctactgaa aatggatctg cagttagtgt aacttctggt
    17821 tgcgcattat taattacaac atctgaagca gtacgatatg ttaatttttc attaaaatca
    17881 atatttttag gtgtatcaat attcgcaaca ttaactttat atgataattt taaagattta
    17941 tctgggaata acacttcttt cgtatgtgta ccacgtgctg ttgtcacacc ttggcttgta
    18001 aatgtaactt tatttgcatt ttgatcataa ttgacagtca tatttttcaa tactgtactg
    18061 tcttcattac cattagggaa tgttgtagtt aatgaatcat taacgtaagt tacaccttgt
    18121 ggtaatgtta cttcatattt aaattgatct gctttcaatg aagcaccaaa attaccattg
    18181 ttaccgattt cagtattaat agtatattct ttattgttag ttgctttcgg tgctaaatct
    18241 ctatttttga catataaatg tgatcctgag aatagaccta cattatcaat gaatgaatag
    18301 tatttataac catctttatt tgttgtaatt cttgaagcat cagcaactgc tgtattgtca
    18361 ggaataaatt ctaaatctaa acgatcagca tctgttggta ctttaaataa gtgtgcagta
    18421 ccagtcttag ctacatttac agttgctaat tcagtatttt tcttagcatc tttaataact
    18481 aaatttgttg caccttgctt actattcgtt tgcatagtag taaagttgat aattaattct
    18541 gaatctggtt ttacattaac agtttttgaa ataccgttaa agttaccgtg atctgtagaa
    18601 tcatttgcat tcacacgacc taatgcagct acattccctc tagcttggta gttttgaggg
    18661 ttattcttat caaacatatc gcttcgtctt aattctgagt taacgaaacc aatcttaccg
    18721 ttgttaatta atgaataacc atttacttta tctgtaacag ttacagttgg atcctgtcta
    18781 ttctcatctg ttgatatggc aggatcatca aacgttaatg ttgtattaat actgccttca
    18841 ccagtattgc tagcatttgg atcttgagtt tgtgcgtttg ctgctacagg tgctgctggt
    18901 tgcgctgctg ctggagcatt cgctggctgt gtttgatttg ccggtgttgt attattatta
    18961 ggtgttgctt ggttatttcc ttgacctgct tggttggcag gtgctgcttg atttccaggt
    19021 tgtgcttgtg cagcgttatt cggatcagct tgattacctt gtccagctgg ttgtgctgta
    19081 cctgctgccg gttgtgctgc gcctcctgct ggattagcct gtccaccttg gtttgctggt
    19141 tgtgctgctg gttgtccttg gttggcaggt gcagctggct gtgctgtagg attagcttga
    19201 gcaccagcat ttgcgttagg ctgtgcattg gcatcagctg gttgtgctgg ttgattttgt
    19261 gcaggctgat tttactctgc tgcagacgct gttgtcgggt taatagatat aaatgtaaca
    19321 gtggcaatta aagctgaaaa aataccgaca ttaaattttc taatactaaa tttttgttgt
    19381 ctgaataaat tcattaagtc atcctcctgg ttgattattc tcgctgttaa atgatgtcac
    19441 ttaatcaact tttaagataa acagtttgct tccatcttat aaaaggtaga gcaactctat
    19501 ctaattaaaa ttaattttat cataattata tattgcttac cagtgaattt tatattacat
    19561 attgattact ttgtttttat gtattcttta ttattttgac tttacgaaat aatattcttg
    19621 ctttagtcaa attaaaaaac cgatgcataa gcatcggttt gatttatatc ttgaaattat
    19681 ttatctaatc ccattttatc ttgaaccaca tcagctattt gttgtgcaaa tctttcagca
    19741 tcttcatcag ttgctgcttc aaccatgaca cgaactaatg gttctgttcc agaaggtctt
    19801 actaaaattc gaccttctcc attcatttct acttctactt tagtcataac ttctttaacg
    19861 tcaacatttt cttcaacacg atatttatct gttacgcgta cgttaattaa tgattgtgga
    19921 tattttttca tttgtccagc taattcactt agtgatttac cagtcatttt tattacagaa
    19981 gctaattgaa taccagttaa taaaccatca ccagttgtat tgtaatccat cataacgata
    20041 tgtccagatt gttctccacc caagttataa ttaccgcgac gcatttcttc tactacatat
    20101 ctgtcgccaa ctttagtttt attagattta attccttctt gttcaagcgc tttgtaaaaa
    20161 cctaagttac tcataacagt agaaacaatc atgtcattat tcaattcttg atttttatgc
    20221 atttcttgac caataataaa cataatttgg tcaccgtcaa cgatttgacc attctcatct
    20281 actgctatga ttctgtctcc atcgccgtca aatgctaacc caaaatcact ttcagtttca
    20341 acaacttttt ctgctaattt ttcaggatgt gtagagccac atttctcatt gatattatat
    20401 ccatcaggac tacatccaat tgtttcagta tctgcctcta aatcaccaaa taagaatggc
    20461 gctagtgatg atgttgaacc atttgcacca tctaaaacaa ttttcaaacc ttcaaagtta
    20521 acatctactg ttgattttaa ataactcaaa tatttttgtg ccccttcaaa gtaatctgaa
    20581 taatgtacaa tatcattgcc aactggtctt ggtaattctg ggttttcttg atccaataat
    20641 gcttcaattt cattttcttg ttcatctgat agtttaaaac catctgatcc aaagaattta
    20701 ataccattat ccgcaactgg attatgagag gctgatatca ttacacctaa ctctgcaccc
    20761 atatcgcgtg ttaaatatgc aacacctggt gttgaaataa tacctaatcg catcacttct
    20821 gcaccaattg aaatcaaacc agctattaat gctgattcta acatttcacc tgaaactcta
    20881 gtatcgcgac ctacaagtac acgtgggtgt ttttcacctt tattatgtgc tagaacatag
    20941 ccaccatatc ttcctaattt aaatgccaat tcaggtgtta gttcttggtt tgcgacacct
    21001 cttactccgt ctgtaccaaa atattttccc attgtttact ctcctttaat ttagctattt
    21061 attttacgtt tatataagcc ttcgtttcac ttggttctgc tttagagaca tgttctggta
    21121 aattgatttt tacagtcttt tcagttgatt ctgaaatacc atctaaatct acttctgcat
    21181 caacttcgct tatattttgc aagtcatctc gactaccgaa gatttcaatt tctttatctt
    21241 ctaagtcaat cgaacttaat tctttatcat ctgctaaact acctttctgt ttaacattta
    21301 ctttaacctt tttgctaaaa ggctctactt taacttgtaa attcacttca ttaggttgaa
    21361 tcgatacatt taatttattc agttttttat caaaagccgt tacttccgcg atatcttttg
    21421 tgtcaccatt aatcttttta ttagttttaa aagtggcttt taaataagcg atatcattca
    21481 attgttcttc tccacctgtt attttaactg tttgtggtga aacttcttgc tttgtaattt
    21541 tataaagtgg atcaatatca ctttgactaa catcaggttg aactgtcatc tttttagtta
    21601 ctttgttttc aagcgtaata tttgccaatt taggttttac agaataatga atgtcatcag
    21661 ctaacccttt aacttgatac ttctcttgat actttccagc tttagcattt gataaatcaa
    21721 tcactactct taaatcttct ggattttcaa tttttattat ctttgattgt ggtcctgaaa
    21781 tagtcacatt aactgtttca ggcgcttttg ttaaatgcaa atctttagtg ttataaagaa
    21841 tttcaacggg tacatcttga atcgttttac tagacttttg accaagatta ccagtgttaa
    21901 agatatttcc aaaaacattg ttaacagata aaaagaaaaa caatgccaaa agaaaggcaa
    21961 taaatcttaa gccccattta ctttctagca tattatttca cacctttctt ttgaaagcgt
    22021 gtgccaaacc aatgttcagc aagcaactct tcaaaaattt cgtttgaaat gtctcggcgt
    22081 aattttccat caaatgttac cgaaatatca ccagtttctt cagatacaat aacggtaaat
    22141 gcatcagata cttctgaaat accaaccgct gctctatgtc ttgtacccaa acttttagat
    22201 atcttaggac tatcagacaa tggcaaataa cttgctgctg ctgcaatctt cgtgccttga
    22261 ataatcattg caccatcatg taaaggtgta ttaggtataa agacattaat taaaagttct
    22321 tgtgaaatat ttgaatccat tgcaatacct gtttcaatat aatcttgaag acctgtttct
    22381 ttttcaaaga caattaatgc acctatacgt cttttagcca tatattgcac agcctttgaa
    22441 accgattgaa tcaatttctc ttcatcttta ctatacgtat tagaagtata gcgttttaaa
    22501 aagctacctc taccaagttg ttctaacgca cgtctaattt ctggttggaa tattactatt
    22561 aaagctaata ccccccattg aataacgata tcgaataatt tagatgttgc agtcaagttc
    22621 aatatcatac ttatctgctg accaataaca attactaata tccctttaag taattgtatc
    22681 gctttagttc ccttaaagac cgtgatgaga agataaagta cataccaaac tatcagtaaa
    22741 tcaaggatac tcgttacaat ttttaacgta ctgaggtttt gaaaaaagtt ggaaaaatcc
    22801 ataacatctc ctccgggtaa tatttttcca taatacccat tataccaatc atttacatca
    22861 ctgtcatacg aacatatatt taaatagaaa aaaatttatt ttaaagatta taactactct
    22921 taatcatttt agtgaattaa aaaaagtagt gcaaaaagca aaatatactt tatacactac
    22981 aaatcattta tttataataa agtttcacca aaaaatgttc caactaacga agccgattgt
    23041 tcagcagtat ggttgttatg atcaataagt ggattcactt caactaagtc cattgaagta
    23101 actgactgag actgatgtaa taattccagc gcaaaatgac tttctctata tgtaagtccg
    23161 cctaagactc tagtacctgt gcctggcgtt tcaagtggat ctaaggcatc aacatctaaa
    23221 gataaatgaa cgccatcaac attgcgtgac ttcaaatatt ctattgtatt ttcaattact
    23281 tcctttatcc ccaatttatc aatatctgac atagtaaatg ttttaatatt atgatctttg
    23341 ataaattgtc tttcaccttt atctaaatct ctcataccaa ttagtacgat gttttctggc
    23401 ttgattacat tactatttaa ttctaaaagt tctttaggac cttcacctgt caaaatcctt
    23461 agaggcatac catgaatatt tccacttggt gactcttcag gtatatttaa atcaccatgt
    23521 gcatcatacc aaataacacc taaattatta taatgtttac ttattgctga tactgaacct
    23581 atcgcaatag aatgatctcc accaagaact agaggaaatc tgttattttc aattgatgct
    23641 gatacttctt tatttaattt ttgagtaaca tctataattt catcgtagtt tcttaatcca
    23701 ttttgttcac tttgaaattt ttcaatgtct acagctgata ccttaatatc ccctttgtca
    23761 ttaacatcaa ggtctaattg ctttagtctt ggtattaaac ctgcatatct aatcgcagtt
    23821 ggtcctaaat caacacctaa ttttctttgt ccaaatgttg acggtacacc aataatatca
    23881 attccttttt tctttgtcat aagcgtcccc ctttgttata cataatttca gaataatgta
    23941 tacgcttact ataatcaagt aatagccata acataacgcc ttagcatcct aattctcaaa
    24001 aaataaaaaa agcacttccc aagaatggaa agtgcaagta gtgagccata gaggattcga
    24061 acctctgacc ctctgattaa aagtcagatg ctctaccaac tgagctaatg gctctaatgg
    24121 ctgggctagc tggattcgaa ccaacgagtg acggagtcaa agtccgttgc cttaccgctt
    24181 ggctatagcc caatatatag atggtggagg ggggcagatt cgaactgccg aacccgaagg
    24241 agcggattta cagtccgccg cgtttagcca cttcgctacc cctccagctt attcagttaa
    24301 ttaataatca aaatggtgga gaatgacggg ttcgaaccgc cgaccctctg cttgtaaggc
    24361 agatgctctc ccagctgagc taattctcca atataatgac tcctacggga ctcgaacccg
    24421 tgttaccgcc gtgaaagggc ggtgtcttaa ccgcttgacc aaggagccat ggctccacag
    24481 gtaggactcg aacctacgac cgatcggtta acagccgata gctctaccac tgagctactg
    24541 tggattaata tta
//
LOCUS       DAKSQE010000028        24539 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.028, whole genome shotgun sequence.
ACCESSION   DAKSQE010000028 DAKSQE010000000
VERSION     DAKSQE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24539
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.028"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            199..519
                     /locus_tag="O0V33_000987"
     CDS             199..519
                     /locus_tag="O0V33_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCX8975636.1"
                     /translation="MPHILNVTVPIPETHVLITKDEYDELIGYSLDPVWNMSDLKKKL
                     KIASDETIKDRLLFHPRFEKELRAQGIVHYPDENFNRWRFNARKMNKFVDEHFNEIYK
                     ERIK"
     gene            516..677
                     /locus_tag="O0V33_000988"
     CDS             516..677
                     /locus_tag="O0V33_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HCX8975637.1"
                     /translation="MSNIYKSYLLAVLCFTVLAIVLMPLLYFTTAWSIAGFASIATFI
                     FYKEYFYEE"
     gene            772..1098
                     /locus_tag="O0V33_000989"
     CDS             772..1098
                     /locus_tag="O0V33_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HCX8975638.1"
                     /translation="MTKNYKDMTQEELRDLLAEKNGELFEVVNEINKETEFAVLLFST
                     VGVSNGDTTSSSHCALGDIVGLANLLNNENDYHDIANVIEMYKLKKLLGLADNKEDEN
                     DVLQNG"
     gene            1079..1339
                     /locus_tag="O0V33_000990"
     CDS             1079..1339
                     /locus_tag="O0V33_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCX8975639.1"
                     /translation="MYYKTGDVCQKIINVDGFDFRLRVKKRAYSVEIVVLDHEGNSID
                     GILVSDENDLYTALDILKQSIYEWIENNTDEQDKLMNLVMKW"
     gene            1348..1611
                     /locus_tag="O0V33_000991"
     CDS             1348..1611
                     /locus_tag="O0V33_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975640.1"
                     /translation="MRDTERNILNIFKTLFDEYTLSNQRALLEIERNHHGYLSINFLH
                     YHDSYKTNNKLVQIHEINPDSHERIKNLIIEVLRGHRKIKKGA"
     gene            1620..3563
                     /locus_tag="O0V33_000992"
     CDS             1620..3563
                     /locus_tag="O0V33_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCX8975641.1"
                     /translation="MKINKLTISNFAGIKEVTFNFDGKDAKIYGNNATGKTTTATALQ
                     WLLFDKGLDGSTKSFNPVPLNEKNAENYELIPTVFAEFEIDGKITTFKKESHPKYTIN
                     QKTNRKEYSRSRTKKQYINDESIKVKDYKARIDELIDEDVFKLITNPQAFNLLDWKKR
                     RSLLFEIAKPINDEDVIKTNDDFKELNNILGDHEIETKKKILTDKIKQINKDIKDIPI
                     RINQTQQNKQDIPEFDNDRYAIIKQEIEQLENERIDIQNGKEEINLRNQLADKQSELK
                     RIEDNNSASNENKIHALTNELHVENGTVANLKTRLKQNKQQITHEENRRNQLLENHKG
                     LKSDLEKSKNQKFEHLDDNVCSCCGQQLPTEQVNEAREKALQKFNVKKSKELETIQTS
                     INHIISEGKKIKPIIEKLEDDNNNLQIKINEAEERSARIQNKINKLKTTHVDVTQTDE
                     YKAVMLEINEINQKRSNIRKTIQDKVSGIDDKISELTQEKSEIEVSRSIEKSNKHLDD
                     VISELRNEEDRLLDEKEKYSHDLYILKEFTTTKVKMLTENINNEFDIAEFKLFNTLVN
                     GELEETCSTTVNGVEYDSGLNNASRINVGLDIINTLSKHFKVTAPIFIDNAESVTELI
                     KTESQQIQLIVNEQDKKLRMETI"
     gene            3565..4485
                     /locus_tag="O0V33_000993"
     CDS             3565..4485
                     /locus_tag="O0V33_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HCX8975642.1"
                     /translation="MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYS
                     PSNAMKQAWLQISQDNKLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQR
                     SYHGNVMMLKRDAGAQDVVAQVIYKGDTFKQEMGETGRIKAIKHEQDFFNIDKENIIG
                     AYCTIVFNDGRDNYIEVMTIEQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVI
                     NRAAKRYINTSTDSNIFKYAQESEQRQRKEVLDAEVEENANQEQLDFEQPVLEEAQYT
                     ELENDKPIDVSDFEEIKEPATEKESEEEPF"
     gene            4566..5183
                     /locus_tag="O0V33_000994"
     CDS             4566..5183
                     /locus_tag="O0V33_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCX8975643.1"
                     /translation="MKFERVQKHFKYKTRHIAGCLITHEHGDHAKYTKQFVDNGVISY
                     MTAGTQQAMNFESHRLCTIKAKQELRIGTWSILPFDIEHDANEPVAFLLQSTLGYKVL
                     YVTDTKYLKYKFNGITHMMLEVNYIYEQMQENIKNGSVHSTLANRIMESHFSLEHAIG
                     MLKANDLTRLEEIHLIHLSSQNSNAKYIKSEIQKVTGAPVYVGGL"
     gene            5184..5654
                     /gene="ssb"
                     /locus_tag="O0V33_000995"
     CDS             5184..5654
                     /gene="ssb"
                     /locus_tag="O0V33_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCX8975644.1"
                     /translation="MLNRTILVGRLTRDPELRTTQSGVNVASFTLAVNRTFTNAQGER
                     EADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVVADSIQF
                     LEPKNSNDTQQDLYQQQVQQTRGQSQYSNNKPVKDNPFANANGPIELNDDDLPF"
     gene            5684..6568
                     /locus_tag="O0V33_000996"
     CDS             5684..6568
                     /locus_tag="O0V33_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCX8975645.1"
                     /translation="MTGWIKLHRKLLDSPIFQNEKLFKVFAYCLMKASHKDHTQLVGR
                     RVVELEKGQFVFGRKRASEELRLKESTVRDYIKLLENLGTIVVKSDNKFSVITVVNWA
                     IYQSMEENSDSKNDNKSTTNQQQMDNKWTTNQQQINTNKNVKNGDNVKNGENEKKKVT
                     AFDFFQDNGFGFITPYNLDDLNYYLDSFENDSDQIVTASLKIAKDRNKVTWGYAKSIL
                     NTWLNANLKSIEQVRAFEKQQLESKKQNYKPFVKQSKEKTPKWLTDSTRETKTPEVDE
                     NLEKDREAFIKRLNSKWE"
     gene            6575..6793
                     /locus_tag="O0V33_000997"
     CDS             6575..6793
                     /locus_tag="O0V33_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975646.1"
                     /translation="MDAFDKYYLFDHDGNKMFSVTPHFKDVRHLVVGLKHTKFNGRRW
                     YLDDYELKTLIDNEQMELGHQTSLFEYI"
     gene            6802..7111
                     /locus_tag="O0V33_000998"
                     /pseudo
     CDS             6802..7111
                     /locus_tag="O0V33_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401967.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
     gene            7124..7492
                     /locus_tag="O0V33_000999"
     CDS             7124..7492
                     /locus_tag="O0V33_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975647.1"
                     /translation="MARKARIVTINDKPYRFSKFEMELIESHGITAGMVSKRVKDGRE
                     LHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERKRKREAELRRKKPHLFNVPQKH
                     PRGRYACYLLENDIFVKVKK"
     gene            7496..7738
                     /locus_tag="O0V33_001000"
     CDS             7496..7738
                     /locus_tag="O0V33_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975648.1"
                     /translation="MTDNARKEYLNQFFGFKRYLYQDNERVAHIHVVNGTYYFHGHIV
                     PGWQSVKKTFDTAEELEIYIKQHGLEYEEQKQLTLF"
     gene            7753..7917
                     /locus_tag="O0V33_001001"
     CDS             7753..7917
                     /locus_tag="O0V33_001001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975649.1"
                     /translation="MNAEKHMQMMQMLQNCVIDKYVSHDEYEELIAIDKHGNKMFIKF
                     YPNTEDDTNE"
     gene            7910..8161
                     /locus_tag="O0V33_001002"
     CDS             7910..8161
                     /locus_tag="O0V33_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCX8975650.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GMTNAIQHSVKEGVELDEAVGIMAGQVVYKYEEEQENEH"
     gene            8151..8333
                     /locus_tag="O0V33_001003"
     CDS             8151..8333
                     /locus_tag="O0V33_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975651.1"
                     /translation="MSISVGDKVFNPETNSTLEIVQLVGDIRDTHYKLSDGSIISLID
                     FVVKPIHLIKEEQEND"
     gene            8326..8868
                     /locus_tag="O0V33_001004"
     CDS             8326..8868
                     /locus_tag="O0V33_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HCX8975652.1"
                     /translation="MTNTLQVRLLSENARMPERNHKTDAGYDIFSAETVVLEPQEKAV
                     IKTDVAVSIPEGYVGLLTSRSGVSSKTHLVIETGKIDAGYHGNLGINIKNDEERDGIP
                     FLYDDIDAELEDGLISILDIKGNYVQDGRGIRRIYQINKGDKLAQLVIVPIWTPELKQ
                     VEEFESVSERGAKGFGSSGV"
     gene            8905..9111
                     /locus_tag="O0V33_001005"
     CDS             8905..9111
                     /locus_tag="O0V33_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCX8975653.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKEKY
                     EKQVKRDAVIKVGQLFENIRECGK"
     gene            9108..9494
                     /locus_tag="O0V33_001006"
     CDS             9108..9494
                     /locus_tag="O0V33_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975654.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGITALLLVVALPIYTVASYQNK
                     EVHQGTITDKYNKRQDKEDKFYIVLDDKQVIENSDLFFKGKFDSADIQARLKVGDKVK
                     VKTIGYRIHFLNLYPVLYEVKKVDKK"
     gene            9491..9640
                     /locus_tag="O0V33_001007"
     CDS             9491..9640
                     /locus_tag="O0V33_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCX8975655.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYVFR
                     AEVSE"
     gene            9640..9840
                     /locus_tag="O0V33_001008"
     CDS             9640..9840
                     /locus_tag="O0V33_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCX8975656.1"
                     /translation="MWITMTIVFAILLLVCISINSDRAREIQALRYMNDYLLDEVVKT
                     KGYNGLKEYRIELKRMNNDIKK"
     gene            9868..10284
                     /locus_tag="O0V33_001009"
     CDS             9868..10284
                     /locus_tag="O0V33_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCX8975657.1"
                     /translation="MIKIEKHDIKKLEEYIQHIDNYRRELKMREYELLESHEPDNAGA
                     GKSNLPGNPIERCAIKKFSDNRYNTLRNIVNGVDRLIDESDEDTLELLRFRYWDCPIG
                     CYEWEDIAHYFGTSKTSILRRRNALIDKLAKYIGYV"
     gene            10516..10815
                     /locus_tag="O0V33_001010"
     CDS             10516..10815
                     /locus_tag="O0V33_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCX8975658.1"
                     /translation="MMTKDERIRFYKSKEWQTTRKRVLERDNYECQQCKRDGKLTTYD
                     KSKRKSLDVDHILSLEHHPEFAHDLNNLETLCIKCHNKKEKRFIKKENKWKDEKW"
     gene            10945..11289
                     /locus_tag="O0V33_001011"
     CDS             10945..11289
                     /locus_tag="O0V33_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975659.1"
                     /translation="MEIIVDENLVLKEKERLQVLYKDIPSNKLKVVDGLIIQAARLRV
                     MLDYMWEDIKEKGDYDLFTQSEKAPPYERERPVAKLFNARDAAYQKIIKQLSDLLPEE
                     KEDTETPSDDYL"
     gene            11286..12947
                     /locus_tag="O0V33_001012"
     CDS             11286..12947
                     /locus_tag="O0V33_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCX8975660.1"
                     /translation="MISNKYVDEYINLWKQGKIILNKERIDLFNYLQKHIYSRDDVYF
                     DEQKIEDCIKFIEKWYFPTLPFQRFIIANIFLIDKNTDEAFFTEFAIFMGRGGGKNGL
                     ISAISDFLSTPLHGVKEYHISIVANSEDQAKTSFDEIRTVLMDNKRNKTGKTPKAPYE
                     VSKAKIINRATKSVIRYNTSNTKTKDGGREGCVIFDEIHYFFGPEMVNVKRGGLGKKK
                     NRRTFYISTDGFVREGYIDAMKHKIASVLSGKVKNSRLFAFYCKLDDPKEVDDRQTWE
                     KANPMLHKPLSEYAKTLLSTIEEEYNDLPFNRSNKPEFMTKRMNLPEVDLEKVIAPWK
                     EILATNREIPNLDNQMCIGGLDFANIRDFASVGLLFRKNDDYIWLGHSFVRQGFLDDV
                     KLEPPIKEWEKMGLLTIVDDDVIEIEYIVDWFLKAREKYGLEKVIADNYRTDIVRRAF
                     EDAGIKLEVLRNPKAIHGLLAPRIDTMFAKHNVIYGDNPLMRWFTNNVAVKVKPDGNK
                     EYIKKDENRRKTDGFMAFVHALYRADDIVDKDMSKALDALMSIDF"
     gene            12963..14150
                     /locus_tag="O0V33_001013"
     CDS             12963..14150
                     /locus_tag="O0V33_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCX8975661.1"
                     /translation="MSILEKIFKTRKDITYMLDLDMIEDLSQQAYVKRLAIDSCIEFV
                     ARAVAQSHFKVLEGNRIQKNDVYYKLNIKPNTDLSSDSFWQQVIYKLIYDNEVLIVVS
                     DSKELLIADSFYREEYALYDDIFKDVTVKDYTYQRTFTMQEVIYLKYNNNKVTHFVES
                     LFEDYGKIFGRMIGAQLKNYQIRGILKSASSAYDEKNIEKLQAFTNKLFNTFNKNQLA
                     IAPLIEGFDYEELSNGGKNSNMPFSELSELMRDAIKNVALMIGIPPGLIYGETADLEK
                     NTLVFEKFCLTPLLKKIQNELNAKLITQSMYLKDTRIEIVGVNKKDPLQYAEAIDKLV
                     SSGSFTRNEVRIMLGEEPSDNPELDEYLITKNYEKANSGENDEKEKDENTLKGGDEDE
                     SGD"
     gene            14134..14871
                     /locus_tag="O0V33_001014"
     CDS             14134..14871
                     /locus_tag="O0V33_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCX8975662.1"
                     /translation="MKAEIKGVIVSNEDKWVYEMLGMDSTCPKDVLTQLEFSDEDVDI
                     IINSNGGNLVAGSEIYTHLRAHKGKVNVRITAIAASAASLIAMAGDHIEMSPVARMMI
                     HNPSSIAQGEAKDLNHAAETLEHVGQIMAEAYAVRAGKNKQELVEMMARETWLNADEA
                     IEQGFADSKMFENDNMQIVASNTQVLSKDVLNRVTALVSKTPEVNIDIDAIANKVIEK
                     INMKEKESEIDVADSKVSANGFSRFLF"
     gene            14895..16040
                     /locus_tag="O0V33_001015"
     CDS             14895..16040
                     /locus_tag="O0V33_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCX8975663.1"
                     /translation="MTINLSETFANAKNEFINAVNNGEPQERQNELYGDMINQLFEET
                     KLQAKAEAERVSSLPKSAQSLSANQRSFFMDINKNVNYKEEKLLPEETIDRIFEDLTT
                     NHPLLADLGIKNAGLRLKFLKSETSGVAVWGKIYGEIKGQLDAAFSEETAIQNKLTAF
                     VVLPKDLNDFGPAWIERFVRVQIEEAFAVALETAFLKGTGKDQPIGLNRQVQKGVSVT
                     EGAYPEKEEQGTLTFANPRATVNELTQVFKYHSTNEKGKSVAVKGNVTMVVNPSDAFE
                     VQAQYTHLNANGVYVTALPFNLNVIESTVQEAGKVLTYVKGLYDGYLAGGINVQKFKE
                     TLALDDMDLYTAKQFAYGKAKDNKVAAVWKLDLKGHKPALEGTEETL"
     gene            16060..16344
                     /locus_tag="O0V33_001016"
     CDS             16060..16344
                     /locus_tag="O0V33_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975664.1"
                     /translation="MVKFKVVRAFKDIEHNQHKYKVGELYPAEGYNNPRVELLTNQIK
                     NKYDKVYIVPLDKLTKQELLELCESLQKKTSSSMVKSEIVDLLNGEDNDD"
     gene            16334..16618
                     /locus_tag="O0V33_001017"
     CDS             16334..16618
                     /locus_tag="O0V33_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCX8975665.1"
                     /translation="MTIDDLLVKFKSLEKIDHNSEDEYLKQLLKMSYERIKNQCGVFE
                     LENLIGQELILIRARYAYQDLLEHFNDNYRPEIIDFSLSLMEVSEDEESV"
     gene            16602..16964
                     /locus_tag="O0V33_001018"
     CDS             16602..16964
                     /locus_tag="O0V33_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HCX8975666.1"
                     /translation="MKKVFKKPRITTKRLNTRVHFYKYTENNGPEAGEKEEKLLYSCW
                     ASIDGVWLRELEQAISNGTQNDIKLYIRDPQGDYLPSEEHYLEIESRYFKNRLNIKQV
                     SPDLDNKDFIMIRGGYSS"
     gene            16961..17365
                     /locus_tag="O0V33_001019"
     CDS             16961..17365
                     /locus_tag="O0V33_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCX8975667.1"
                     /translation="MSVKVIGDKALERELEKRFGIKEMVKVQDKALIAGAKVIVEEVK
                     KQLKPSKDTGALINEVSFSKPEWINGKRTITVHWRGSKDRYKIVHLIEYGHVQKGTGK
                     FIKPKAMGGVNRAIRQGQNKYFETLKRELKKL"
     gene            17362..17769
                     /locus_tag="O0V33_001020"
     CDS             17362..17769
                     /locus_tag="O0V33_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975668.1"
                     /translation="MIDILYKVHEVISQDRIIREHVNINNIKFNKYPNVKDTDVPFIV
                     IDDIDDPIPTTYTDGDECAYSYIVQIDVFVKYNDEYNARIIRNKISNRIQKLLWSELK
                     MGNVSNGKPEYIEEFKTYRSSRVYEGIFYKEEN"
     gene            17770..18414
                     /locus_tag="O0V33_001021"
     CDS             17770..18414
                     /locus_tag="O0V33_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCX8975669.1"
                     /translation="MAVKHASAPKAYINITGLGFAKLTKEGAELKYSDITKTRGLQKI
                     GVETGGDLKTAYADGGPIESGNTDGEGKISLQMHAFPKEIRKIVFNEEYDEDGVYKET
                     QGKQNNYVAIWFRQERRDGTFRTVLLPKVMFTNPKIDGETAEKDWDFSSEEVEGEALF
                     PLVDNKKSVRKYIFDSANMTNHDGDGEKGEEAFLKKILGEEYTGNVTEGNEETL"
     gene            18456..18680
                     /locus_tag="O0V33_001022"
     CDS             18456..18680
                     /locus_tag="O0V33_001022"
                     /inference="COORDINATES: protein motif:HMM:NF014427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCX8975670.1"
                     /translation="MPDSIKTLKVGDTYDLNVVVEPSNQSKLLKYTTDQTNIVSINSD
                     GQVTAEAQGIATVKATVGNMSDTITINVEA"
     gene            18730..19080
                     /locus_tag="O0V33_001023"
     CDS             18730..19080
                     /locus_tag="O0V33_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975671.1"
                     /translation="MAKLKRNIIQLVEDPKANEIKLQTYLTPHFISFEIVYEAMDLID
                     DIEDENSTMKPREIADRLMDMVVKIYDNQFTVKDLKERMHAPDGMNALREQVIFITQG
                     QQTEETRNFIQNMK"
     gene            19325..23854
                     /locus_tag="O0V33_001024"
                     /pseudo
     CDS             19325..23854
                     /locus_tag="O0V33_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            23851..>24539
                     /locus_tag="O0V33_001025"
     CDS             23851..>24539
                     /locus_tag="O0V33_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCX8975672.1"
                     /translation="MIDTIKVNNKTIPWLYVERGFEIPSFNYVLKTENVDGRSGSIYK
                     GRRLESYSFDIPLVVRNDYLSHNGIKTHDDVLNELVKFFNYEEQVKLQFKSKDWYWNA
                     YFEGPIKLHKEFTIPVKFTIKVVLTDPYKYSVTGNKNTAISDQVSVVNSGTADTPLIV
                     EARAIKSSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHSEFRDFKGWTKMITEDI
                     PSNDLGGKVGG"
BASE COUNT         9438 a         3431 c         4949 g         6721 t
ORIGIN      
        1 tttatgttgt ttctattaat tatatctaat tggtacttag gttttaaaga ttgtttaatt
       61 gccatacttt tagaaatttc aacattacta attacgttat taatagaaaa acgaacattt
      121 tttaaaggat caatatacac caatatcacc tcctttcact aggagataac aacattatac
      181 acgaaaggaa agatagaaat gccacatatt ttaaacgtaa cagttccaat acctgaaaca
      241 catgtactta tcacaaaaga tgaatatgat gagctaattg gttattcatt agaccctgta
      301 tggaacatga gtgacttaaa gaagaaatta aaaattgcat ctgatgagac tatcaaggac
      361 agattactat ttcatcctag atttgaaaaa gaactaagag cgcaaggaat tgtgcattac
      421 ccagatgaga attttaatcg ctggagattt aacgcaagaa agatgaataa attcgtcgat
      481 gagcatttca atgaaatata taaggagaga ataaaatgag caacatttat aaaagctacc
      541 tattagcagt actgtgcttc acagtcttag cgattgtgct tatgccattg ctgtacttca
      601 ctacagcatg gtcaattgca ggattcgcaa gtatcgcaac attcatattc tataaggaat
      661 acttttatga agaatgaaaa aactgctact tgcgccaaca agtaacagtg acaaacgatt
      721 aacaaaatta attcattttc aatataaaac gaaaaacgga ggaagtcaac tatgactaaa
      781 aattataaag acatgacgca ggaagaatta agagatttat tggctgaaaa gaatggagaa
      841 ttgtttgaag tagtgaatga aatcaataaa gaaactgaat ttgccgtttt acttttttca
      901 actgtagggg ttagcaatgg agatactaca tcatcgtcac attgtgcgct tggggatatt
      961 gtaggtcttg ctaatttatt gaataacgaa aatgattacc acgatatcgc taatgttatc
     1021 gaaatgtata aattaaaaaa acttttaggt ctagctgaca acaaggagga cgagaatgat
     1081 gtattacaaa acgggtgacg tatgtcaaaa aataattaat gtagatggct ttgattttcg
     1141 attaagagtt aagaaacgag catatagcgt cgaaatagtt gttttagatc atgaggggaa
     1201 ttcaattgac gggatactag tttctgacga gaacgatcta tacacagcgt tagatatttt
     1261 gaaacaaagt atttatgaat ggattgaaaa taacacagat gaacaggaca aactaatgaa
     1321 cttagtcatg aaatggtagg tataagcatg agagacacag aaagaaatat attgaatatt
     1381 tttaagacgt tattcgacga atatactttg tcaaaccaac gagcattatt ggaaattgaa
     1441 cgtaatcatc acggatactt atcgattaat ttcctgcact atcacgacag ttacaaaaca
     1501 aacaataagc ttgtgcagat acatgaaatc aatccagaca gccatgaacg aataaaaaat
     1561 ttaattatcg aggtgctaag aggtcatcgg aagattaaaa aaggagcatg aggaaagata
     1621 tgaaaataaa taagttaact atatcgaact ttgctggaat caaagaagta acatttaact
     1681 ttgacggtaa agatgcaaaa atatacggca ataatgcgac tggtaagact acaacagcaa
     1741 ccgcattaca atggctgctt ttcgataaag gtttggacgg atcaaccaaa tcatttaacc
     1801 ctgtaccttt aaacgaaaaa aacgcagaaa attatgagtt aattccgact gttttcgcag
     1861 aatttgaaat cgacggaaaa attacgactt ttaaaaaaga gtcacatcct aaatacacaa
     1921 taaatcaaaa aacgaatcgc aaggaatact cacgaagtcg aacgaagaaa caatatatca
     1981 atgatgaatc aataaaagta aaggattata aagctcgtat tgatgaactg attgatgaag
     2041 atgtattcaa gttaattacg aaccctcaag catttaactt actagattgg aagaagcgaa
     2101 gaagtttgtt gtttgaaatt gctaaaccaa tcaatgatga ggatgtcatt aaaacaaatg
     2161 atgattttaa agaattaaat aatattcttg gagatcatga aattgaaaca aagaaaaaga
     2221 ttcttacgga caagataaaa cagattaaca aagatatcaa agatattccg atacgtatta
     2281 accaaacaca acaaaataag caggatatac cagaattcga taacgataga tacgcaatta
     2341 tcaaacaaga aattgagcaa cttgaaaatg agcgtataga tattcaaaac ggtaaggaag
     2401 aaattaattt gcgtaatcaa ttagctgata aacaatcaga attgaaacgc atagaagaca
     2461 ataacagcgc aagtaatgag aacaaaatcc atgctttaac aaatgaatta cacgttgaaa
     2521 atggaacggt agcaaacctt aaaacgagat taaagcaaaa caaacaacaa atcacacatg
     2581 aagaaaatag acgtaatcaa ttattggaaa atcacaaagg actaaaaagt gatttagaaa
     2641 aatctaaaaa tcaaaaattt gaacatcttg atgacaatgt atgtagttgt tgtggtcaac
     2701 agttaccaac tgaacaagtg aatgaggcaa gagaaaaagc tttacagaaa ttcaatgtaa
     2761 aaaaatcgaa agaattagaa acaatacaaa catctatcaa tcacattatt tcagaaggca
     2821 agaaaataaa gccaatcatc gagaagttag aggatgacaa taataatctt caaattaaaa
     2881 tcaacgaagc agaagagcgt tcagcaagaa tacaaaacaa aattaataag ttgaaaacga
     2941 ctcacgttga cgttacgcaa actgacgaat acaaagcagt aatgttagag ataaatgaga
     3001 ttaatcaaaa acgctctaac atcaggaaaa ctattcaaga taaagtttca ggaatagatg
     3061 acaaaataag cgaacttact caagaaaaat cagaaattga agtgtcaaga tcaatcgaaa
     3121 aatcaaataa acatctagat gatgttattt ctgaattaag aaatgaagaa gacagattat
     3181 tggatgaaaa agaaaagtat tcacatgacc tttatatctt aaaagaattt acaacaacaa
     3241 aagtcaaaat gcttactgaa aatatcaata acgaatttga tattgctgaa tttaagttat
     3301 tcaatacctt agttaacggc gaattagaag aaacatgttc cacaacggtt aacggcgtcg
     3361 aatacgacag cggtttaaat aacgcctcaa gaattaatgt tggcttagat atcatcaata
     3421 cactgtcaaa acattttaaa gttacagcac caatatttat tgataatgct gaatcagtaa
     3481 cagagcttat caaaacagaa tcacaacaaa ttcaattgat agtaaatgaa caagataaaa
     3541 aattaagaat ggagactata taaaatgact gaaaataata aattacaaac tattgaacaa
     3601 caattagtac aagaaaagaa cgtatctgac aacgtattaa acaaagtgag agttttagag
     3661 tcacaaggca atttggaatt gccaaatgat tattcaccaa gtaatgccat gaaacaagca
     3721 tggttacaaa tcagccaaga taacaaatta atgagttgta acgatacaag caaagcaaat
     3781 gccttattag acatggtaac gcaaggttta aatccagcta aaaatcaatg ctactttatt
     3841 ccttacggca acaaaatgca gttacaacgt agctatcacg gtaatgtaat gatgttaaaa
     3901 cgtgatgcag gtgctcaaga tgttgttgct caagtgattt ataaaggcga tacattcaag
     3961 caagaaatgg gagaaacagg acgtatcaaa gcgattaaac acgaacaaga cttctttaac
     4021 atcgacaaag aaaacattat cggtgcgtac tgcacaatcg tatttaatga tggacgagat
     4081 aactatattg aagtcatgac tattgaacaa attaaacaag catggatgca gtcatcaatg
     4141 attaaagatg aaaaagcatt acaaaattct aaaacacata ataatttcaa agaagaaatg
     4201 gctaaaaaaa cagttatcaa tagagctgct aaacgttata tcaacacatc aacagatagc
     4261 aatattttca aatacgcaca agaatccgaa caacgtcaac gcaaagaagt gttggacgca
     4321 gaagttgaag aaaatgcaaa tcaagaacaa ttggactttg aacaaccagt tcttgaagaa
     4381 gcacaataca cagaattaga aaatgataag cctattgatg tatctgactt tgaagaaata
     4441 aaagaacctg caacagaaaa agaaagcgaa gaagagccat tttaattgaa acaatagcaa
     4501 ctggttcaag tggtaactgc tacgtcttaa atgatggacg tactacgtta ctacttgagg
     4561 caggtataaa atttgaacgt gttcaaaagc attttaaata taaaacaaga catatagcag
     4621 ggtgtcttat cacacacgaa catggtgatc atgcaaagta cacaaagcag tttgtcgaca
     4681 atggtgtaat cagctatatg actgctggaa cacaacaagc tatgaatttt gaaagtcatc
     4741 gcttatgcac gattaaggca aagcaagagc tgcgaatagg cacatggtca attctaccgt
     4801 ttgacatcga acatgatgct aacgagcctg tggctttctt attacaaagt acattaggtt
     4861 ataaggttct gtatgttact gatacaaagt atttgaaata caaatttaac ggcattacgc
     4921 acatgatgtt agaagttaat tatatctatg aacaaatgca ggaaaacata aaaaacggca
     4981 gtgtgcacag cacattagca aacagaatta tggagtctca ttttagctta gaacatgcta
     5041 tcggaatgtt aaaagcaaat gatttaacta gactcgaaga aatacattta attcatttaa
     5101 gtagtcaaaa ttcaaatgca aaatacatta aaagtgaaat acaaaaagtg acgggcgcgc
     5161 ccgtttatgt tggaggttta taaatgctaa acagaacaat attagttggt cgtttaacta
     5221 gagacccaga attaagaacc actcaaagtg gtgtaaatgt agcatcattc acattagcag
     5281 ttaaccgcac atttacgaat gcacaaggag agcgcgaggc agactttatt aatatcatcg
     5341 tatttaaaaa acaagcagag aacgttaata aatacctatc taaaggatcg ttggcgggcg
     5401 tagatggtag gttacaaacg cggaactatg aaaataagga aggtcaacgt gtatacgtta
     5461 cggaagttgt tgccgatagt attcaatttt tagaaccgaa gaactcaaat gacactcaac
     5521 aagatttata tcaacaacaa gtacaacaaa cacgtggaca atcgcaatat tcaaataaca
     5581 aaccagtaaa agataatccg tttgcgaatg caaatggtcc gattgaacta aatgatgatg
     5641 atttaccatt ctgatttaac cggtttgaaa gtgaggtgtg tatatgactg gttggataaa
     5701 acttcataga aaactattag attcgcctat ttttcagaac gaaaagttat tcaaagtatt
     5761 tgcatattgt cttatgaagg ctagtcataa ggatcataca cagcttgttg gcagacgagt
     5821 tgttgaatta gaaaaaggtc aatttgtgtt cgggagaaag cgagcaagcg aagagttacg
     5881 tctcaaagaa tccacagtaa gagactacat aaagctttta gaaaaccttg gaactatcgt
     5941 cgtaaagtcc gacaacaaat tttctgttat aaccgttgtc aattgggcga tttatcaaag
     6001 tatggaagaa aattccgaca gcaaaaacga caacaaatca acaacaaatc aacaacaaat
     6061 ggacaacaaa tggacaacaa atcaacaaca aatcaacaca aacaagaatg taaagaatgg
     6121 ggataatgta aagaatggtg agaatgagaa gaagaaggta accgccttcg acttcttcca
     6181 agataacgga ttcggtttca taactcctta caatttagac gatttaaatt attatcttga
     6241 ttcatttgaa aatgattcag atcaaatagt taccgcatca cttaaaatcg ctaaagacag
     6301 aaataaagtt acttggggat atgctaaaag cattttgaat acatggctta atgcaaactt
     6361 gaaatctatt gaacaagtac gtgcatttga aaagcaacaa cttgaaagca aaaaacaaaa
     6421 ttataaacct ttcgttaaac aatcaaaaga aaaaacaccc aaatggctca cagacagcac
     6481 gagagaaacg aaaacgccgg aagtagatga aaaccttgag aaagacagag aagcttttat
     6541 taagcgtcta aatagcaaat gggagtgatt gaaaatggat gcatttgata aatactatct
     6601 atttgatcat gacggcaaca aaatgttttc agttacacca cattttaaag atgtacggca
     6661 tttagttgtt ggattaaaac acacaaaatt caatggtcga cgttggtact tagatgatta
     6721 tgaattaaaa acacttattg ataatgaaca aatggagtta ggacaccaaa caagcttatt
     6781 tgaatatata tgagggatta catggagata gaaattaaat ttaacgaaac tttcgaggca
     6841 cctatgggct cgcctcgacc gcgttttagc acaaaaggta gatatgcaca cacatatatg
     6901 cctacaaaat atacagaaca taaaaaatat ttacaaaatc aaatgccaaa gctaaatcta
     6961 gaaaatgcat taaaaattga attagagttt tacttttcta gacgcttgta acaattattt
     7021 gtggaaagat gacaatcaaa ttgcagaaat aactagctca aagcgttatg gaattgagcc
     7081 caaaataatc atacgaatag aagaaatata agaggtggaa taaatggcga gaaaagcaag
     7141 aattgtaaca ataaacgata aaccttatag gttcagtaaa tttgaaatgg aattaataga
     7201 aagtcacggt ataaccgctg gaatggtttc taagagagta aaagacggtc gggaactaca
     7261 tgaagcaatg gacgcaccag aaggtacgcg tttaagcgag tacagagaaa agaaaacaat
     7321 agaaagactg gaacaagcta gactcgaacg caaattggaa agaaagcgaa agagagaggc
     7381 tgagctaaga agaaagaagc cacacttgtt taatgtacct cagaaacatc caagaggacg
     7441 ttatgcgtgc tacctgttgg aaaacgacat attcgtgaaa gttaagaagt agatcatgac
     7501 agataacgca cgcaaagaat acctaaatca attctttgga tttaagagat atctgtatca
     7561 ggataacgaa cgagtggcac atattcatgt agtaaacggc acttattact ttcatgggca
     7621 tatcgtgcca ggttggcaaa gcgttaaaaa gacatttgat actgctgaag agctcgaaat
     7681 atatataaag caacatggtt tggaatacga ggaacagaag caactaactt tattttagag
     7741 gagatggaaa tgatgaatgc tgaaaagcat atgcaaatga tgcaaatgtt acaaaattgt
     7801 gtgattgata agtatgtatc acacgacgaa tacgaagagt taattgccat agataagcat
     7861 ggtaataaaa tgtttattaa attttatccg aatacggagg atgacactaa tgaataatcg
     7921 cgaacaaatt gaacaatcag ttataagtgc tagtgcgtat aacggtaatg acacagaggg
     7981 attactaaaa gagattgagg acgtttataa gaaagcgcaa gcgtttgatg aaatacttga
     8041 gggaatgaca aatgctattc aacattcagt taaagaaggt gttgaacttg atgaagcagt
     8101 agggattatg gcaggtcaag ttgtctataa atatgaggag gaacaggaaa atgagcatta
     8161 gtgtaggaga caaggttttt aatccagaaa caaattcaac tttagaaatt gtacaacttg
     8221 ttggcgatat tagagacacg cattacaagt tatctgacgg atctattatt agtcttatag
     8281 actttgttgt taaaccaatt catttaatca aggaggagca ggaaaatgac taacacatta
     8341 caagtaaggc tattatcaga aaatgctaga atgcccgaac gaaatcataa gacggatgca
     8401 ggttatgaca tattctcagc tgaaactgtc gtacttgagc cacaagaaaa ggcagtgatt
     8461 aaaacagatg tagctgtaag cataccagag ggctatgtcg ggctattaac tagccgtagt
     8521 ggtgtaagta gtaaaacaca tttagtgatt gaaacaggca agatagacgc cggatatcac
     8581 ggcaatttag ggattaatat taagaatgat gaagaacgtg atggaatacc ctttttatat
     8641 gatgatatag acgctgaatt agaagatgga ttaataagca ttttagatat aaaaggtaac
     8701 tatgtacaag atggaagagg cataagaaga atttaccaaa tcaacaaagg cgacaaacta
     8761 gcacaactgg ttatcgtgcc tatatggaca cctgaactaa agcaagtgga ggaattcgag
     8821 agtgtttcag aacgtggagc aaaaggcttc ggaagtagcg gagtgtaaag acatcttaga
     8881 tcgagtcaag gaggttttgg ggaagtgaca caatacttag tcacaacatt caaagattca
     8941 acaggacgta aacatacaca cataactaaa gctaagagta atcaaaggtt tacagttgtt
     9001 gaggcagaga gtaaagaaga agcgaaagag aagtacgaga aacaagttaa aagggatgca
     9061 gttattaaag tgggtcagtt gtttgaaaat ataagggagt gtgggaaatg attaaaaaac
     9121 ttaaaaatat ggatgggttc gacatcttta ttgttggaat actgtcatta ttcggtataa
     9181 ccgcattgct acttgttgtc gcattgccta tctatacagt ggctagttac caaaacaaag
     9241 aagtacatca agggacaatt acagataaat ataacaaaag acaagataaa gaggacaaat
     9301 tctatattgt attagatgat aaacaagtca tcgaaaactc agacttattc ttcaaaggaa
     9361 agtttgatag cgcagacata caagctaggt taaaagtagg tgataaagta aaagttaaga
     9421 cgattggata tagaatacac tttttaaatt tatatccggt cttatacgaa gtaaagaagg
     9481 tagataaaaa atgattaagc aaatactaag attattattc ttactagcga tgtatgagtt
     9541 aggtaagtat gtaactgagc aagtatatat tatgatgacg gctaatgatg atgtagaggc
     9601 gccgagtgat tacgtctttc gagcggaggt aagtgagtga tgtggattac tatgactatt
     9661 gtatttgcta tattgctatt agtttgtatc agtattaata gtgatcgtgc aagggagata
     9721 caagcgctca gatatatgaa tgattatcta cttgatgaag tagttaaaac taaaggatac
     9781 aacgggttaa aagaatacag gattgaatta aagcgaatga ataacgatat taaaaagtaa
     9841 tttatattat cggaggtatt gcattgaatg ataaagattg agaaacacga tatcaaaaag
     9901 cttgaagaat acattcagca catcgataac tatcgaagag agttgaagat gcgagaatat
     9961 gaattacttg aaagtcatga accagataat gcgggagctg gcaaaagtaa tttgccgggt
    10021 aacccgattg aacgatgtgc aataaagaag tttagtgata acaggtacaa tacattaaga
    10081 aatatagtta acggtgtaga tagattgata gatgaaagtg atgaggatac gcttgagtta
    10141 ttaaggttta gatattggga ttgtcctatt ggttgttatg aatgggaaga tatagcacat
    10201 tactttggta caagtaagac aagtatatta cgtagaagga atgcactgat cgataagtta
    10261 gcaaagtata ttggttatgt gtagcggact tttaccctat gtaagtccgc attaaaacag
    10321 tttattatgt tagtatcaga ttaatattta aagttattaa atgctaatac gacgcatgaa
    10381 caagaggcgc atcactatgt gatgtgtctt tttatttatg aggtatgaac atgttcaaac
    10441 taatagtaaa tacattacta cacatcaagt atagatgcgt cttgatacta cttaagttat
    10501 ataaggtgaa acattatgat gactaaagac gaacgcatac gattctataa gtctaaagaa
    10561 tggcaaacaa caagaaaaag agtgctagaa agagataatt atgaatgtca acaatgtaag
    10621 agagacggca agttaacgac atatgacaaa agcaagcgta agtcgttgga tgtagatcat
    10681 atattatcgc tagaacatca tccggagttt gctcatgact taaacaattt agaaacactg
    10741 tgtattaaat gtcacaacaa aaaagaaaag agatttataa aaaaagaaaa taaatggaaa
    10801 gacgaaaaat ggtaaatacc cccgggtcaa aaaaatcaaa agcgatcaaa atacttgggg
    10861 aacgggcagg ggctcgactt cgcgataatt ttaaaaatcc atgtataacc cccctcttat
    10921 aaccatttta aggcaggtga tgaaatggag attatagttg atgaaaactt agtgcttaaa
    10981 gaaaaagaaa ggctgcaagt attatataaa gacataccta gcaataaatt aaaagtagtt
    11041 gatggtttaa ttattcaagc agcaaggcta cgtgtaatgc ttgattacat gtgggaagac
    11101 ataaaagaaa aaggtgacta tgatttattt actcaatctg aaaaggcgcc accatatgaa
    11161 agggaaagac cagtagccaa actatttaat gctagagatg ctgcatatca aaaaataatc
    11221 aaacaattat cggatttatt gcccgaagag aaagaagaca cagaaacgcc atctgatgat
    11281 tacctatgat tagtaataaa tacgttgatg aatatataaa tttgtggaaa caaggaaaga
    11341 taattttaaa taaagaaaga attgatctct ttaattatct acaaaaacat atatattcac
    11401 gagatgatgt atattttgat gaacagaaaa tcgaggattg tatcaaattt attgaaaaat
    11461 ggtattttcc aacattacca tttcaaaggt ttatcatagc taatatattt cttatagata
    11521 aaaatacaga tgaagctttc tttacagaat ttgctatttt catgggacgt ggaggcggga
    11581 aaaacggtct aataagtgct attagtgatt ttctttctac gcccttacac ggagttaaag
    11641 aatatcacat ctccattgtt gctaatagtg aagatcaagc aaaaacatcg tttgatgaaa
    11701 tcagaaccgt tttaatggat aacaaacgaa ataagacggg taaaacgcca aaagctcctt
    11761 atgaagttag taaagcaaaa ataataaacc gtgcaactaa atcggttatt cgatataaca
    11821 catcaaacac aaaaaccaaa gacggtggac gtgaggggtg tgttattttt gatgaaattc
    11881 attatttctt tggtcctgaa atggtaaacg tcaaacgtgg tggattaggt aaaaagaaaa
    11941 atagaagaac gttttatata agtactgatg gttttgttag agagggttat atcgatgcaa
    12001 tgaagcacaa aattgcaagt gtattaagtg gcaaggttaa aaatagtaga ttgtttgctt
    12061 tttattgtaa gttagacgat ccaaaagaag ttgatgacag acagacgtgg gaaaaggcga
    12121 acccaatgtt acataaaccg ttatcagaat acgctaaaac actgctaagt acgattgaag
    12181 aagaatataa cgatttacca ttcaaccgtt caaataagcc cgaattcatg actaagcgaa
    12241 tgaatttgcc tgaagttgac cttgaaaaag taatagcacc atggaaagaa atactagcga
    12301 ctaatagaga gataccaaat ttagataatc aaatgtgtat tggtggttta gactttgcaa
    12361 acattcgaga ttttgcaagt gtagggctat tattccgaaa aaacgatgat tacatttggt
    12421 taggacattc gtttgtaaga caagggtttt tggatgatgt caaattagaa cctcctatta
    12481 aagaatggga aaaaatggga ttattgacca ttgtcgatga tgatgtcatt gaaattgaat
    12541 atatagttga ttggttttta aaggctagag aaaaatatgg gcttgaaaaa gtcatagctg
    12601 ataattatag aactgatatt gtaagacgtg cgtttgagga tgctggcata aaacttgaag
    12661 tacttagaaa tccaaaagca atacatggat tacttgcacc acgtatcgat acaatgtttg
    12721 cgaaacataa cgtaatatat ggagacaatc ctttgatgcg ttggtttact aataatgttg
    12781 cagtaaaggt taaacccgat ggtaataaag aatatattaa aaaagatgaa aatagaagaa
    12841 aaaccgatgg gttcatggct tttgttcacg cattatatag agcagacgat atagtagaca
    12901 aagacatgtc taaagcgctt gatgcattaa tgagtataga tttctaatag aggaggtgag
    12961 acatgagtat tctagaaaag atatttaaaa ctaggaaaga tataacatat atgcttgatt
    13021 tagatatgat agaagatcta tcacaacaag cgtatgtgaa acgtttagcg attgatagtt
    13081 gtattgaatt tgttgcgcga gctgtcgctc aaagtcattt taaagtattg gaaggtaata
    13141 gaattcaaaa gaatgatgtt tactacaagt taaatataaa accaaatact gacttatcaa
    13201 gcgatagttt ttggcaacaa gttatatata aactaattta tgataacgag gttttaatcg
    13261 tagtaagtga cagcaaagaa ttacttatcg cagatagctt ttacagagaa gagtacgctt
    13321 tgtatgatga tatattcaaa gatgtaacgg ttaaagatta tacttatcaa cgtactttca
    13381 caatgcaaga ggtcatatat ttaaagtaca acaacaataa agtgacacac tttgtagaaa
    13441 gtctattcga agattacggg aaaatattcg gaagaatgat aggtgcacaa ttaaaaaact
    13501 atcaaataag agggattttg aaatctgcct ctagcgcata tgacgaaaag aatatagaaa
    13561 aattacaagc gttcacaaat aaattattca atacttttaa taaaaatcaa ctagcaatcg
    13621 cgcctttgat agaaggtttt gattatgagg aattatctaa tggtggtaag aatagtaaca
    13681 tgcctttttc tgaattgagt gagctaatga gagatgcaat aaaaaatgtt gcgttgatga
    13741 ttggtatacc tccaggtttg atttacggag aaacagctga tttggaaaaa aacacgcttg
    13801 tatttgagaa gttctgttta acacctttat taaaaaagat tcagaacgaa ttaaacgcga
    13861 aactcataac acaaagcatg tatttgaaag atacaagaat agaaattgtc ggtgtgaata
    13921 aaaaagaccc acttcaatat gctgaagcaa ttgacaaact tgtaagttct ggttcattta
    13981 caaggaatga ggtgcggatt atgttaggtg aagaaccatc agacaatcct gaattagacg
    14041 aatacctgat tactaaaaac tacgaaaaag ctaacagtgg tgaaaatgat gaaaaagaaa
    14101 aagatgaaaa cactttgaaa ggtggtgatg aagatgaaag cggagattaa aggcgtcatc
    14161 gtttccaacg aagataaatg ggtttacgaa atgcttggta tggattcgac ttgtcctaaa
    14221 gatgttttaa cacaactaga atttagtgat gaagatgttg atattataat taactcaaat
    14281 ggtggtaacc tagtagctgg tagtgaaata tatacacatt taagagctca taaaggcaaa
    14341 gtgaatgttc gtatcacagc aatagcagca agtgcggcat cgcttatcgc aatggctggt
    14401 gaccacatcg aaatgagtcc ggttgctaga atgatgattc acaatccttc aagtattgcg
    14461 caaggagaag cgaaagatct aaatcatgct gcagaaacat tagaacatgt tggtcaaata
    14521 atggctgagg catatgcggt tagagctggt aaaaacaaac aagaacttgt agaaatgatg
    14581 gctagggaaa cgtggctaaa tgctgatgaa gccattgaac aaggttttgc ggatagtaaa
    14641 atgtttgaaa acgacaatat gcaaattgta gcaagcaata cacaagtgtt atcgaaagat
    14701 gtattaaatc gtgtaacagc tttggtaagt aaaacgccag aggttaacat tgatattgac
    14761 gcaatagcaa ataaagtaat tgaaaaaata aatatgaaag aaaaggaatc agaaatcgat
    14821 gttgcagata gtaaagtatc agcaaatgga ttttcaagat tcctttttta atacaaaaaa
    14881 taggaggtca taaaatgact ataaatttat cggaaacatt cgcaaatgcg aaaaacgaat
    14941 ttattaatgc agtaaacaac ggtgaaccgc aagaaagaca aaatgaattg tacggtgaca
    15001 tgattaacca actatttgaa gaaactaaat tacaagcaaa agcagaagct gaaagagttt
    15061 ctagtttacc taaatcagca caatctttga gtgcaaacca aagaagtttc ttcatggata
    15121 tcaataaaaa cgttaactat aaagaagaaa aacttttgcc agaagaaaca attgatagaa
    15181 tttttgaaga tttgacgacg aatcatccgt tattagctga tttaggtatt aaaaacgctg
    15241 gtttgcgttt gaagttctta aaatctgaaa cttctggcgt agccgtttgg ggtaaaatct
    15301 atggtgaaat taaaggtcaa ttagatgctg cgttcagtga agaaacagca attcaaaata
    15361 aattgacggc gtttgttgtt ttaccaaaag atttaaatga ttttggtcct gcgtggattg
    15421 aaagatttgt tcgtgttcaa atcgaagaag catttgcagt ggcgcttgaa actgcgttct
    15481 taaaaggtac tggtaaagac caaccaatcg gcttaaaccg tcaagtacaa aaaggtgtat
    15541 cggtaactga gggtgcttat ccagagaaag aagaacaagg tacgcttaca tttgctaatc
    15601 cgcgcgctac ggttaatgaa ttgacgcaag tgtttaaata ccactcaact aacgagaaag
    15661 gtaaatcagt agcggttaaa ggtaatgtaa caatggttgt taatccgtcc gatgcttttg
    15721 aggttcaagc acagtataca catttaaatg caaatggcgt atatgttact gctttaccat
    15781 ttaatttgaa tgttatcgag tctacagtcc aagaagcagg taaggtttta acgtacgtta
    15841 aaggtttata tgatggttat ttagctggtg gtattaatgt tcagaaattt aaagaaacac
    15901 ttgcgttaga tgatatggat ttatacactg caaaacaatt tgcttacggc aaagcgaaag
    15961 ataataaagt tgctgctgtt tggaaattag atttaaaagg acataagcca gctttagaag
    16021 gtaccgaaga aacactataa aattttatga ggtgataaaa tggtgaaatt taaagttgtt
    16081 agagctttta aagacataga gcacaatcaa cacaagtaca aagtagggga gttgtatcca
    16141 gctgaagggt ataacaatcc tcgtgttgaa ttgttgacaa atcaaatcaa aaataagtac
    16201 gacaaagttt atatcgtacc tttagataag ctgacaaaac aagaattatt agaactatgc
    16261 gaatcattac aaaaaaaaac gtctagttca atggttaaaa gtgaaatcgt cgacttattg
    16321 aatggtgaag acaatgacga ttgatgattt gcttgtcaaa tttaaatcac ttgaaaagat
    16381 tgaccataat tcagaggatg agtacttaaa gcagttgtta aaaatgtcgt acgagcgtat
    16441 aaaaaatcag tgcggagttt ttgaattaga gaatttaata ggtcaagaat tgatacttat
    16501 acgcgctaga tatgcttatc aagatttatt agaacacttc aacgataatt acagacctga
    16561 aataatagat ttttcgttat ctctaatgga ggtatcagaa gatgaagaaa gtgtttaaaa
    16621 aacctagaat tacaactaaa cgtttaaata ctcgtgttca tttttataag tatactgaaa
    16681 ataatggtcc agaagctgga gaaaaagaag aaaaattatt atatagctgt tgggcgagta
    16741 ttgatggtgt ctggttacgt gaattagaac aagctatctc aaacggaacc caaaatgaca
    16801 ttaaattgta tattcgtgat ccgcaaggtg attatttacc cagtgaagaa cattatcttg
    16861 aaattgaatc aagatatttc aaaaatcgtt tgaatataaa gcaagtatca ccagatttgg
    16921 ataataaaga ctttattatg attcgtggag gatatagttc atgagtgtga aagtgatagg
    16981 tgataaagca ttagaaagag aattagaaaa acgttttggc ataaaagaga tggtaaaagt
    17041 tcaagataag gcgttaatag ctggtgctaa ggtaattgtt gaagaagtaa aaaaacaact
    17101 aaagccctca aaagatacgg gagcattaat taatgaggta agttttagta aacctgaatg
    17161 gataaacgga aaacgtacaa ttactgttca ttggcgaggt tctaaagacc gttataaaat
    17221 cgtacattta attgaatatg gacacgttca aaaaggaaca ggtaaattta tcaaacctaa
    17281 agctatgggc ggtgttaata gagcaataag acaagggcaa aataagtatt ttgagacgct
    17341 aaaaagggag ttgaaaaaat tgtgattgat attttgtaca aagttcatga agtgattagt
    17401 caagacagaa ttattagaga gcacgtaaat atcaataata ttaagttcaa taaataccct
    17461 aatgtaaaag atactgatgt accttttatt gttattgacg atatcgacga cccaatacct
    17521 acaacttata ctgacggaga tgagtgtgca tatagttata ttgtccaaat agatgttttt
    17581 gttaagtaca atgatgaata taatgcgaga atcataagaa ataagatatc taatcgcatt
    17641 caaaagttat tatggtctga actaaaaatg ggaaatgttt caaatggaaa accggaatat
    17701 atagaagaat ttaaaacata tagaagctct cgcgtttacg agggcatttt ttataaggag
    17761 gaaaattaaa tggcagtaaa acatgcaagt gcgccaaagg cgtatattaa cattactggt
    17821 ttaggtttcg ctaaattaac gaaagaaggc gcggaattaa aatatagtga tattacaaaa
    17881 acaagaggat tacaaaaaat tggtgttgaa actggaggag acttgaaaac agcatatgct
    17941 gatggtggtc caatcgaatc agggaacaca gacggagaag gtaaaatttc gttacaaatg
    18001 catgctttcc ctaaagagat tcgcaaaata gtattcaatg aagagtatga tgaagacggt
    18061 gtttataaag aaactcaagg taaacaaaac aactatgtag caatttggtt cagacaagag
    18121 cgtcgagacg gcacatttag aacggtttta ttacctaaag tcatgtttac aaatcctaaa
    18181 atcgatggag aaacggctga gaaagattgg gatttctcaa gtgaagaggt tgaaggtgag
    18241 gcacttttcc ctttagttga taataaaaag tcagtacgta agtatatctt tgattcagct
    18301 aacatgacaa atcatgatgg agacggtgaa aaaggcgaag aggctttctt aaagaaaatt
    18361 ttaggcgaag aatatactgg aaacgtgaca gagggtaacg aagaaacttt gtaacaaaac
    18421 cggcttcatc ggaaactgcg gtaaagtcgg ttaatatacc agatagcatt aaaacactta
    18481 aagttggcga cacatacgat ttaaatgttg tagtagagcc atctaatcaa agtaagttat
    18541 tgaaatacac aacagatcaa acgaatattg tatcaatcaa tagtgatggt caagttactg
    18601 cggaagcaca aggcattgct acggttaaag caacagttgg taatatgagt gacactataa
    18661 caataaatgt agaagcataa gagggggcaa cccctctatt ttatttgaaa ataaggagag
    18721 tattataaaa tggcaaaatt aaaacgtaac attattcaat tagtagaaga cccgaaagca
    18781 aatgaaatta aattacaaac gtacttaaca ccacacttca tttcatttga aattgtatac
    18841 gaagcaatgg atttaatcga tgatattgag gacgaaaata gcacgatgaa accaagagaa
    18901 atcgctgaca gattgatgga tatggttgta aaaatttacg ataaccaatt cacagttaaa
    18961 gacctaaaag aacgtatgca tgcacctgat ggaatgaatg cacttcgtga acaagtgatt
    19021 ttcattactc aaggtcagca aactgaggaa actagaaatt ttatccagaa catgaaataa
    19081 agcctgaaga tttaacatat aaagcaatgt tgaaaaatat ggatactctc atgatggact
    19141 taattgaaaa tggtaaagac gctaacgaag ttttaaaaat gccatttcat tatgtacttt
    19201 ccatatatca aaataaaaac aatgacattt ctgaagaaaa agcagaggct ttaattgatg
    19261 cgttttaacc ttaaccgttt ggttagggtt atttttttga acttttttag aaaggaggta
    19321 aaaaatggga gaaagaataa aaggtttatc tataggtttg gatttagatg cagcaaattt
    19381 aaatagatca tttgcagaaa tcaaacgaaa ctttaaaact ttaaattctg acttaaaatt
    19441 aaccggtaac aacttcaaat ataccgaaaa atcaactcat agttacaaac aaaggattaa
    19501 agaacttgat ggaactatca caggttataa gaaaaacgtt gatgatttag ccaagcaata
    19561 tggcaaggta tctcaagaac agggcgaaaa cagcgcggaa gctcaaaaat tacgacaaga
    19621 atataacaaa caagcaaatg agctgaattt tttagaaaaa gaactagaaa aaacaacaac
    19681 tgagtttgaa gagttcaaaa aagctcaagt tgaagctcaa agaatggcag aaagtggctg
    19741 gggaaaaacc agtaaagttt ttgaaagtat gggacctaaa ttaacaaaaa tgggtgatgg
    19801 tttaaaatcc attggtaaag gtttgatgat tggtgtaact gcacctgttt taggtattgc
    19861 agcagcatca ggaaaagctt ttgcagaagt tgataaaggt ttagatacag ttacccaagc
    19921 aacaggagca accggcggag agcttaagaa gttgcagaat tcatttaaag atgtttatgg
    19981 caactttcca gcagacgctg agactgtagg cggtgtttta ggggaagtta acacaaggtt
    20041 aggtttcact ggcaaagaac ttgagagtgc cacagagtca ttcttgaaat ttagtcacat
    20101 aacaggttct gacggcgtac aagccgttca attaattacg cgtgcaatgg gtgatgcagg
    20161 tattgaagct gatgagtatc aaagtgtact tgatatggta gcgaaagcag cacaggctag
    20221 cggtataagt gttgatacat tagctgatag cattactaaa tacggtgctc caatgagggc
    20281 tatgggcttt gagatgaaag aatcaatcgc tttattctct caatgggaga aatcaggtgt
    20341 taatactgaa atagccttca gtggtttgaa aaaagctata tccaattggg gtaaagcggg
    20401 taaagaccca agagaagaat ttaagaagac attagcagaa attgaaagga caccggatat
    20461 agctagcgca acaagtttag cgattgaagc atttggtgca aaagcaggtc ctgatttagc
    20521 agatgctatt aaaggcggtc gctttagtta ccaagagttc ttaaaaacta tcgaagattc
    20581 gcaaggaacg gtcaatcaga catttaaaga ttctgaaagt ggctccgaaa gatttaaagt
    20641 agcaatgaat aaacttaaat tagtaggtgc tgatgtatgg gcttctattg aaagtgcgtt
    20701 tgctccagtc atggaagaat taatcaaaaa gctatctgta gcagttgatt ggttttcaag
    20761 tttaagtgat ggatctaaaa ggtcgattgt tatattcggt ggtattgctg ctgcaattgg
    20821 tcctgtagtt tttggattag gtgcattcat aagcacaatt ggcaacgcag taactgtatt
    20881 agctccatta ttagctagta ttgtaaaggc tggcggattg attagttttt tatcaactaa
    20941 agtgcctatt ttaggaacag tcttcacagc attaactggt ccaattggta tcgtgttagg
    21001 tgtactggct ggtttagcag tcgcatttac aatagcttat aagaaatctg aaacattcag
    21061 aaattttgtt aatggtgcaa ttaacagtgt taaacaaacg tttagtaatt tcattcaatt
    21121 tatccaacct ttcattgatt ccgttaaaaa cgtctttaaa caagcggttt cagcaatcgt
    21181 tgatttcgct aaagatattt ggagtcaaat taatggattc tttaatgaaa acggaatttc
    21241 tattgttcaa gcgcttcaaa atatatgcaa ttttatcaaa gctatatttg aatttatctt
    21301 aaattttgta attaaaccaa tcatgtttgc gatttggcaa gtgatgcaat ttatttggcc
    21361 ggcggttaaa gccttgattg tcagtacttg ggagaatata aaaggagtaa tacaaggtgc
    21421 tttaaatatc atacttggct ttattaagtt cttttcaagt ttattcactg gtaattggcg
    21481 aggtgtttgg gacggtattg tgatgatact aaaaggcact gtgcagttaa tttggaattt
    21541 aatacaactg tggtttgtag gtaagattct aggtgttgtt agatactttg gtggattgct
    21601 taaaggttta atatccggta tctggggtgt tatcaaaggt attttcacaa aatcattatc
    21661 tgcaatttgg aatgcaacga aaagtatttt tggtttctta tacaatagtg ttaaatctat
    21721 tttcactaat atgaaaaact ggttatctag tacgtggaat aatatcaaaa gcaataccgt
    21781 cggcaaggct cattcgttat ttacgggtgt aaggtctaaa ttcacaagtt tatggaatgc
    21841 gacgaaagat atatttacta aattaagaaa ttggatgtca aacatctgga actctattaa
    21901 agataacacg gtaggtatag cgggtcgctt atgggataga gtgcgtaaca tctttggaag
    21961 catgcgtgac ggtttaaaat ctatcattag taaaattaaa gatcatatcg gtggtatggt
    22021 agacgctgtt aaaagaggtc ttaataaatt aattgaaggt ttaaactggg tcggtggtaa
    22081 gttgggtatg gacaaaatac cgaagttaca cactggtact gaacatacac atactactac
    22141 aagattagtt aagaacggta agattgcacg tgacacattc gctacagttg gggataaggg
    22201 acgcggaaat ggtccaaatg gtttcagaaa tgaaatgatt gaattcccta atggtaaacg
    22261 tgtaatcaca ccaaatacag atactacggc ttatttacct aaaggctcaa aagtatacaa
    22321 cggggcacaa acttattcaa tgttaaacgg aacgcttccg agatttagtt taggtactat
    22381 gtagaaagat attaaatctg gtgcatcatc ggcatttaac tggacaaaag ataaaatagg
    22441 taaaggtacc aaatggcttg gcgataaagt tggcgatgtt ttagatttta tggaaaatcc
    22501 aggcaaactt ttaaattata tacttgaagc ttttggaatt gatttcaatt ctttaactaa
    22561 aggtatggga attgcaggcg acataacaaa agctgcatgg tctaagatta agaaaagtgc
    22621 tactgattgg ataaaagaaa atttagaagc tatgggcggt ggcgatttag tcggtggaat
    22681 attagaccct gacaaaatta attatcatta tggacgtacc gcagcttata ccgctgcaac
    22741 tggaagacca tttcatgaag gtgtcgattt tccatttgta tatcaagaag ttagaacgcc
    22801 gatgggtggc agacttacaa gaatgccatt tatgtctggt ggttatggta attatgtaaa
    22861 aattactagt ggcgttatcg atatgctatt tgcgcatttg aaaaacttta gcaaatcacc
    22921 acctagtggc acgatggtaa agcccggtga tgttgttggt ttaactggta ataccggatt
    22981 tagtacagga ccacatttac attttgaaat gaggagaaat ggaagacatt ttgaccctga
    23041 accatattta aggaatgcta agaaaaaagg taggttatca attggtggcg gtggcgctac
    23101 ttctggaagt ggtgcaactt atgccagccg agtaatccga caagcacaaa gtattttagg
    23161 aggacgttat aaaggtaagt ggattcatga ccagatgatg cgagttgcaa agcgcgaaag
    23221 taactatcaa tcaaatgcag tgaataattg ggacattaat gctcaaagag gagacccgtc
    23281 tagaggatta ttccaaatta tcggctcaac ttttagagct aacgctaaac gagggtacac
    23341 taattataat aatccagtac atcaaggtat ctcagcaatg cagtacattg ttagacgata
    23401 tggttggggt ggttttaaac gtgctggtga ttacgcatat gctacaggtg gaaaagtttt
    23461 tgatggttgg tataacttag gtgaagacgg tcatccagaa tggattattc caacagatcc
    23521 agctcgtaga aatgatgcaa tgaagatttt gcattatgca gcagcagaag taagagggaa
    23581 aaaagcgagt aaaaataagc gtcctagcca attatcagac ttaaacgggt ttgatgatcc
    23641 tagcttatta ttgaaaatga ttgaacaaca gcaacaacaa atagctttat tactgaaaat
    23701 agcacaatct aacgatgtga ttgcagataa agattatcag ccgattattg acgaatacgc
    23761 ttttgataaa aaggtgaacg cgtctataga aaagcgagaa aggcaagaat caacaaaagt
    23821 aaagtttaga aaaggaggaa ttgctattca atgatagaca ctattaaagt gaacaacaaa
    23881 acaattcctt ggttgtatgt cgaaagaggg tttgaaatac cctcttttaa ttatgtttta
    23941 aaaacagaaa atgtagatgg acgttcgggg tctatatata aagggcgtag gcttgaatct
    24001 tatagttttg atataccttt ggtggtacgt aatgactatt tatctcacaa cggtattaaa
    24061 acacatgatg acgtcttgaa tgaattagta aagtttttta actacgagga acaagttaaa
    24121 ttacaattta aatctaaaga ttggtactgg aacgcttatt ttgaaggacc aataaagctg
    24181 cacaaagaat ttacaatacc tgttaagttc actatcaaag tagtactaac agacccttac
    24241 aaatattcag taacaggaaa taaaaatact gcgatttcag accaagtttc agttgtaaat
    24301 agtgggactg ctgacactcc tttaattgtt gaagcccgag caattaaatc atctagttac
    24361 tttatgatca ctaaaaatga tgaagattat tttatggttg gtgatgatga ggtaaccaaa
    24421 gaagttaagg attacatgcc tcctgtttat catagtgagt ttcgtgattt caaaggttgg
    24481 actaagatga ttactgaaga tattccaagt aatgatttag gtggtaaggt cggcggtga
//
LOCUS       DAKSQE010000029        24024 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.029, whole genome shotgun sequence.
ACCESSION   DAKSQE010000029 DAKSQE010000000
VERSION     DAKSQE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24024
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.029"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            169..1362
                     /gene="fmtA"
                     /locus_tag="O0V33_001026"
     CDS             169..1362
                     /gene="fmtA"
                     /locus_tag="O0V33_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HCX8975673.1"
                     /translation="MKFNKEKLVIHACVLLFIIISIGLVFHRLQTKTNSIEPIHKETK
                     LSDNAKYLVDRNKGKGEPSKLKEVYNSKDPKYKKIDRYLQNSLFNGSVAVYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYIPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVRAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHSAFYDEKSFRKYFAKGYSYNSTGLSFLKP
                     NVLEQYYGAGNIYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKQYPDEYRYGFY
                     VKPTLNRLNGGLFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTK
                     GVIVQ"
     gene            complement(1705..1983)
                     /gene="qoxD"
                     /locus_tag="O0V33_001027"
     CDS             complement(1705..1983)
                     /gene="qoxD"
                     /locus_tag="O0V33_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HCX8975674.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFIQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(1992..2597)
                     /gene="qoxC"
                     /locus_tag="O0V33_001028"
     CDS             complement(1992..2597)
                     /gene="qoxC"
                     /locus_tag="O0V33_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HCX8975675.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGFHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(2587..4575)
                     /gene="qoxB"
                     /locus_tag="O0V33_001029"
     CDS             complement(2587..4575)
                     /gene="qoxB"
                     /locus_tag="O0V33_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HCX8975676.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNGYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(4575..5675)
                     /gene="qoxA"
                     /locus_tag="O0V33_001030"
     CDS             complement(4575..5675)
                     /gene="qoxA"
                     /locus_tag="O0V33_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HCX8975677.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
     gene            5980..6099
                     /locus_tag="O0V33_001031"
     CDS             5980..6099
                     /locus_tag="O0V33_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975678.1"
                     /translation="MERKCKHFNEEFAKSNLSLNNEKRKFVCSKSKHSFKKSS"
     gene            complement(6247..6564)
                     /locus_tag="O0V33_001032"
     CDS             complement(6247..6564)
                     /locus_tag="O0V33_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HCX8975679.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
     gene            complement(7346..8206)
                     /gene="folD"
                     /locus_tag="O0V33_001033"
     CDS             complement(7346..8206)
                     /gene="folD"
                     /locus_tag="O0V33_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCX8975680.1"
                     /translation="MVAKILDGKQIAKDYRQGLQNQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPDKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
     gene            8407..8889
                     /gene="purE"
                     /locus_tag="O0V33_001034"
     CDS             8407..8889
                     /gene="purE"
                     /locus_tag="O0V33_001034"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCX8975681.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKTM
                     VQFASEARERGIDIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            8876..9997
                     /gene="purK"
                     /locus_tag="O0V33_001035"
     CDS             8876..9997
                     /gene="purK"
                     /locus_tag="O0V33_001035"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCX8975682.1"
                     /translation="MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVAVLDPAEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESKDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGIKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITYFPLQENEHRNQI
                     LFKTIVPARIDKIAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGE"
     gene            10005..10709
                     /locus_tag="O0V33_001036"
     CDS             10005..10709
                     /locus_tag="O0V33_001036"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCX8975683.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHIKLLNIATDKDIETLKTKALEINQVLK
                     QLMHAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            10709..10972
                     /gene="purS"
                     /locus_tag="O0V33_001037"
     CDS             10709..10972
                     /gene="purS"
                     /locus_tag="O0V33_001037"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCX8975684.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLEDEKENA"
     gene            10974..11645
                     /gene="purQ"
                     /locus_tag="O0V33_001038"
     CDS             10974..11645
                     /gene="purQ"
                     /locus_tag="O0V33_001038"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HCX8975685.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPVISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQKLKANN
                     QIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            11638..13827
                     /gene="purL"
                     /locus_tag="O0V33_001039"
     CDS             11638..13827
                     /gene="purL"
                     /locus_tag="O0V33_001039"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCX8975686.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGEHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGIGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYEDEVYADIPVEPLADEAPVYILEGEEKNYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEALYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLADSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPIVGMVGLIENVDYLNDF
                     QPQVGDKLYVIGDTKDDFGGSQLEKLIYGKVNHEFESIDLSSEVKKGESIKTAIRQGV
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSLIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            13806..15290
                     /locus_tag="O0V33_001040"
     CDS             13806..15290
                     /locus_tag="O0V33_001040"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCX8975687.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPLLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKIKGGFTFAILTKDALYGAVDPNDIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKM
                     LAQESPVKADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHYFDSEGNLNV"
     gene            15283..16311
                     /locus_tag="O0V33_001041"
     CDS             15283..16311
                     /locus_tag="O0V33_001041"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCX8975688.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIETNEMYNIFNMGIGYTVIVDEKDAPRALKILAEQNVEAYQIGHIVKNES
                     TAIELLGV"
     gene            16314..16880
                     /gene="purN"
                     /locus_tag="O0V33_001042"
     CDS             16314..16880
                     /gene="purN"
                     /locus_tag="O0V33_001042"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCX8975689.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFGSKAAYEQHLVTLLNEDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRQCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            16895..18373
                     /gene="purH"
                     /locus_tag="O0V33_001043"
     CDS             16895..18373
                     /gene="purH"
                     /locus_tag="O0V33_001043"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCX8975690.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPAAVAVKHMNPCGVGIGDTLEQAFHHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALNILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPGGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            18395..19642
                     /locus_tag="O0V33_001044"
     CDS             18395..19642
                     /locus_tag="O0V33_001044"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCX8975691.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVH
                     TEISESDHQGILDFAKQQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCEFPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGELAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNEAIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVHDAQRDAYEK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(19910..20716)
                     /locus_tag="O0V33_001045"
     CDS             complement(19910..20716)
                     /locus_tag="O0V33_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCX8975692.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDVIFIIVLIAIIILAYYCSITLPITGIDDVRLGRVG"
     gene            complement(20709..22109)
                     /locus_tag="O0V33_001046"
     CDS             complement(20709..22109)
                     /locus_tag="O0V33_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8975693.1"
                     /translation="MLKVSDLRLKYPSGQRKVFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQVP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQHLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLENVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            complement(22124..22699)
                     /locus_tag="O0V33_001047"
     CDS             complement(22124..22699)
                     /locus_tag="O0V33_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCX8975694.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     regulatory      complement(22776..22876)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(23283..23417)
                     /gene="graF"
                     /locus_tag="O0V33_001048"
     CDS             complement(23283..23417)
                     /gene="graF"
                     /locus_tag="O0V33_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HCX8975695.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEEKTEDINQTKQDIQDTLN
                     "
     gene            23569..>24024
                     /locus_tag="O0V33_001049"
     CDS             23569..>24024
                     /locus_tag="O0V33_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975696.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVHNVWEFLNAFFSEIIKVMSRFIGNFPIVSAIVIIIFGILVMLIGHTLLRTIKYDY
                     DISIFFLVIGIMYFIITLILMTQVYGFFAVIFIIPFTIHIGYIVYKDELN"
BASE COUNT         8398 a         3618 c         4423 g         7585 t
ORIGIN      
        1 tgataaggta taattaaatt tgttgtaata agagcttata tgaagaaaat attactaagt
       61 agtataacgt ataagtgggt gacagatgaa attgtaagtt atattataat gaagttaagt
      121 ttattgtttt aattatttaa tttgaataca atgagaggct attagcgaat gaaatttaat
      181 aaagagaaat tggttataca tgcgtgcgtg cttttgttca tcattatctc cataggatta
      241 gtttttcatc gattacaaac gaaaacgaac tctatagaac ctatacataa agaaacaaaa
      301 ttatcggata atgcgaaata tttggttgat cgtaataaag gcaaaggtga gccatcgaaa
      361 ttaaaggaag tatacaatag taaagatcca aagtataaga agattgaccg gtatttacaa
      421 aattcattat ttaatggttc tgtagcggta tatgaaaatg gcaaattgaa aatgagtaag
      481 ggctatggat atcaagattt tgaaaaagga attaaaaata caccaaatac gatgtttcta
      541 attggttcag ctcaaaaatt ttcaacagga ttactgttaa aacagttaga agaagaacat
      601 aaaattaata ttaatgatcc agtaagcaaa tatattccat ggtttaaaac atctaagcct
      661 attcctttaa aagacttgat gctgcatcaa agtggattat ataaatataa atcttcaaaa
      721 gactataaga acttagatca agcagtgaga gcaattcaaa aacgtggcat tgatcctaag
      781 aaatacaaaa agcatatgta taatgatgga aattatttag tgctagctaa agttatcgaa
      841 gaagttaccg gcaaatcata tgccgagaat tactatacta aaataggtga tccattaaaa
      901 cttcagcatt cggcatttta tgatgaaaaa tcatttagaa aatattttgc aaaaggttat
      961 tcttataata gtacaggact ttctttcttg aagccaaatg ttttagaaca atattatggt
     1021 gcaggtaata tatacatgac accaacagat atgggtaaat taattactca gatacaacaa
     1081 tataaattat ttagtcctaa aataaccaat ccattattac atgagtttgg tacgaaacag
     1141 tatccagatg aatatcgata tggtttctat gttaagccaa cattgaatag acttaacggg
     1201 ggactcttcg gacaagtctt tactgtttac tataatgata agtatgtagt tgtgcttgca
     1261 ttaaatgtaa aaggaaacaa tgaagttcga atcaaacata tttataatga tattttaaaa
     1321 caaaataaac cttataatac gaagggtgtt attgttcaat aattaatata gaagatataa
     1381 cttgtatatg gcattaaggc attgacctta tttgactagt atacgagtta tatcttcttt
     1441 tttatagagg tgaaaagttt aaaggataag gttgaagtag tagggtgata aatttgcgtt
     1501 aagttaaaaa gatataaaca attgatgtga ctgtcaatat aaaaatgtac ataaatgatt
     1561 gtataagaat gtattaattg aatggataat aatgattgtc ttattagata tagaagggga
     1621 cttaaccatt taaaaattga aagataataa aaaccagcta acaaaagtgt tagctggtta
     1681 aactttacat tattaaaatg tggtttataa gtgtgaagag tgaccgcctt gcataaccca
     1741 atatgttccg acaacgaaac aaagtgtgat tacaagagca aagataactt tgaatgtttg
     1801 taaacgtcca tctttacctt cagttaaatg catgaacatt aataattgaa gtccagcttg
     1861 gatgaatgca aagccaaaga taattgtcaa cttcgcgtgg aatgttaatg acgtgtatag
     1921 tgttacgtaa actgctaaaa gcgttaatac gatagatgcg ataaatccta cagtatgttt
     1981 cattattgta ctcatccgct atacaccatc cctatcatat atacggcagt aaagatgaaa
     2041 acccaaacaa catctaagaa gtgccagtat aaacttacta taaataattt tggcgcatta
     2101 tatttgtcta atccgcgtcg ttggatttgg attaataaac aaatggccca aacgatacct
     2161 agcgatacgt gaaaaccatg cgttcctaat aggataaaga aactagacca gtaagaacca
     2221 attgttgggt taacgccttc tgatgcatag tgtgcgaatt cataaatttc gaatccaaca
     2281 aagactaaac ctaaaagtaa cgtaatgatc atccaaaaca tcattaactt ttgtttttct
     2341 tggcgcatgt agtaaatagc aataccacat gtgtaagaac tgaataataa tgcaaacgtc
     2401 attattaaaa caagaggcaa ttcaaataac tcagtagtca ttttacctgc ataatcgcca
     2461 ccatgttgca aagttaatag tgtcgcaaat agggtaccga ataacgcaaa ttcggctgta
     2521 atgaaaatcc aaaagccaag cttatttaat tcgccttcat gtgtgcgtga atcaatagtg
     2581 tttgtatcat gactcatgac ttacagcctc cctttcttta atacgcgctt ctcttaatct
     2641 agcttcagtt tcagcaactt cagcagcagg gatatgatat ccgtgatcga tttggaaact
     2701 gcgataaatc atagtaccaa aaataccgaa taaacaaatt aatgctggaa ttacagtttc
     2761 gaaaattaag aagaaaccgc cgatagtcat aaagatacca atccagaatc caacaggagt
     2821 attgtttggc atatgaatgt ctttgtaatt atggttgtct aaataatgac gaccatgttc
     2881 tttcatatca acaaatgtgt cgtagccatt ccaatctggt gtaatggcaa agttgtattt
     2941 aggtggaatt gctgatgcag tagtccactc tagagtacga ccaaggccat cccagttatc
     3001 tccagttgct tcacgtggag atttgaagtg actgtatacg atactaacaa caaggaataa
     3061 gaatccgatt gccattaata atgcaccgat agttgaaatt aagtttaata agaaccaacc
     3121 atctgatggc atataagtgt ataaacgacg tggcatacca tctaatccaa gaatgaattg
     3181 tggtaagaaa caaacgttaa atccgatcat gaagaaccag aagcaccatt tgtttaatgt
     3241 ttcgtttaat ttgtaaccca tcatctttgg ataccagaag attaaaccag ctaagcaggc
     3301 aaatacaaca ccagtaacca atgtatagtg gaagtgagct actaagaagt acgtattgtg
     3361 atattgatag tcagcagatg ccattgctaa cattacaccc gtaacaccac ctaaaaggaa
     3421 gttagggatg aatgctaatg agaataacat tggtgactca aatgtaatac gtcctttata
     3481 taatgttaat aaccagttaa acaatttcac accagttgga ataccaatca gcattgttga
     3541 aattgagaag aatgagttga ttaacgcacc attacccatt gtgaagaaat ggtgaaccca
     3601 aactaagaaa ctaaggaaag cgataccggc agttgcccat accatacttt gatgtccgaa
     3661 taaacgctta cgagcgaatg tcggaataat ttctgagtaa ataccaaatg ctggaaggat
     3721 aacgatataa acttcagggt gcccccatac ccagaagaag ttagcccaaa gcattggcat
     3781 accaccatgt gcaactgtga agaatgctgt gtcaaatatt ctatcagttg tcattaatgc
     3841 taacgctact gttaaaggag ggaaagcaag aataacaatt aatgtagtaa taaatgttgt
     3901 tactgtaaac attggcattt gcataaactt catagttggt gttttacatc ttaaaattgt
     3961 tacaaagaag ttgatacctg tagctaaggt accaagccct gaaatttgta tagctattaa
     4021 gtaatagtta acacccggac caggactgaa ttcacctgct agtggtgcat agtttgtcca
     4081 accagctgct ggtgaaccac caataataaa tgacaggttg aataaaatca tacctgcaaa
     4141 gaatagccag aaacttacgt tgtttaatac tgggaatgca acatcacgtg ctccaatttg
     4201 taatggaaca acgatattcc ataaaccaaa gataaatggc attgccatga agataatcat
     4261 gattacacca tgtgtactaa aaatttcgtt atagtggtta gattctaaaa atttgttatc
     4321 aggtactgtt agttgcgcac gaataagtaa cgcatcaata ccaccacgga cgaacattaa
     4381 tacggcacag attaaataca taataccgat tttcttatgg tctacagatg tgaaccattc
     4441 tttgtaaaga tatttccata atttaaagta agtaattact gcgattaaac caataactaa
     4501 gaatggggca ccaatttgtg ccattgtaat catccagtta cctttaacta gtaattgatc
     4561 ccatggaaaa ttcattaatg tccacctcca tgatcatcat tgtcttgatc ttgcgcatct
     4621 tttgaaattt tcttcatttc tttcgcattt ttcgattcat ctttcttgaa ctcattgtta
     4681 tatggttcgt catttccaag aatcatcaat ttcataccat gtcgtttata gttcgcattt
     4741 gtaatttgag ctttacgagc aggtattaat ggtttgtctg atacatcttt aaacatattt
     4801 tcttcactag tgaagtttgg atctttcaat tcgaaattga aacgtttata tgcatagaag
     4861 atgtattctg gatcggctgc tggatcaaca aacgccatat gtgtaccatt aaattctaaa
     4921 gctttattag gtgtgcttgg taataattgt ttatcaaatg tatcttgatc taacgttttc
     4981 ttacctttaa cttctttcac ccatttgtcg tagtcttttt gactaacggc atttacttta
     5041 aatgtttgac gcgagaatcc ttcaccattg aagttagaat tacgacctct gaacgtacca
     5101 gtttgagatg cttctaacgt ccaattcatt gtcatgccag tcatggcata tttttgacca
     5161 cctaattgtg gaatccagaa acttgtcatt gtatccatag cttgaagctt aaatacaaca
     5221 ggacgatctt tagggattgt taatgtatta acagtctcta tatgttcatc tggataagca
     5281 aagaaccatt tgtatcctgc acttactgca tatacaacca ttggatcttt ctcactcttc
     5341 ggtggttttt cgtaatcgta taaagtttta actgtaggaa tagctaaagc agcaacgatt
     5401 atgataggta ttacaaacca tattgtttca atgatggcat tatggtgcat cttaccagat
     5461 tcggcattct tattataact atacttgtaa ataaaaatgg cgaacatgcc aagtacaacg
     5521 aaacaaataa caagcatgaa gacgattgaa taaagaatca agaacttctg actacttgct
     5581 actggccctt ttgcgttgaa aatttctata tttgaacaac cactaagtaa aattagtgtg
     5641 ccaaataata gaagcaaaga cttaaatttt gacacttttt tgacctccta atactacaaa
     5701 tgtagggctt aacattaatt ttaagttatt acacaatatt tacaagggct tatgggaaaa
     5761 aaattaataa aattgtatca aaaatgttga taaatcaagg tgtgacgtgg gttcacacat
     5821 ttgttaaaaa tatgtgtaca ttttgtgact aatagcgttt ttgaaaaaat tgcttcatat
     5881 ttaaaaggga agttgagaat agctttcaat taaaggttaa ttgaatgaaa ataaaaaaag
     5941 tttgaaattg tctaataaat tgaaaatgat ggctagttca ttgaaaggaa gtgcaaacat
     6001 tttaatgagg aatttgccaa aagtaattta tcgttaaaca atgaaaagag gaaatttgtt
     6061 tgttcaaaat caaaacattc ttttaaaaag agcagttaag ttttaataat ttgataggta
     6121 gtcctttggt atacagaaaa aatacaatat tggggttaaa gttgcagatg aagttggaat
     6181 aacaaataaa atggtagcaa aacatgtacg tttgctacca ttttatttgc agtatagtga
     6241 tgagggctat ttaacctcaa tatacctagt ggaaatttta attgcaccat ctgaatcagt
     6301 gacatgatat ttaatttgat atttaccaga tttagatgta tcaatttggc catcgacttt
     6361 aattttatcg gttaaatctc catcttcttt atcaaatgca cttatgccgt ttagaaggtt
     6421 ataatcttga cctttcttaa taacgatatc attagcgcct ttaatttgtg gtgtagtatt
     6481 cgtcgttgca tctgcattta aatttggtgt tactagtgta gcagaaacac cgagggctga
     6541 taatgactgt aatagtttat tcataatgta acctccgaat gataagtcat ttctgagtta
     6601 gctttactat accttaattt atattattta cacatcatac tatagtccta atcttaccct
     6661 ataaagaatg aaatttaaat ataaatttat aaataatgag gaaataatta ttattttgaa
     6721 aatctgccac aattgtaaaa ttaataattc agggataatt tgaaatcaaa tataacttta
     6781 ttcgacagtt tgaacttaaa ttgttgtgca tcataacaat aagaaaaggg aggcaaaaat
     6841 aataaagaac cactaatgat ttattatgta gtagtatttt atcattagtc acagcttctg
     6901 tgttggggac tcgctgactt gcattgtctg tagaaattgg gaatccaatt tctctgtgtt
     6961 ggggcccatc ctcaacttgt acattattgt aagctgactt ttcgtcagct tctgtgttgg
     7021 ggcccacacc ccaacttgca ttgcctgtag aatttctttt tgaaattctc tgtgttgggg
     7081 cccctgacta gaattgaaaa cttgttacaa gcttaatttc atttaattaa ctattgtcta
     7141 tataaaattg ttgagcctag aacaatgtga aatgctccct tcaaagcctt catttattca
     7201 atgtctactt tgaagggagc atttgatcga ttaatgtccc agatttttct tgttattcat
     7261 tgcaattgca aaataatatt taatttgggt taaaagagtt cattaaaact catttattaa
     7321 ttcacattac ataacttatg tgaatttacg aatcaatacc tcgacgcatt ttttctgcaa
     7381 gcaaagtatt atttaatacc atagtaattg ttaatggacc aacgccacca ggaactggtg
     7441 taatagctcc agcaatttct ttaaccgcat cataatcaac gtcacctttt aatttgccat
     7501 tttcatctgg cgtattgcca acatctataa ttactgctcc ttctttgaca acatcttttg
     7561 ttactaaacc aggcttacca actgcactga caatgacatc agcatctttt aaatatgatg
     7621 ccatatcctt tgaacgagaa tgtaagattg ttactgatgc atttttttga agtagtaact
     7681 tagaaactgg ttgtccgaca atatgacttc gtccgattac aactgcattt ttaccttcta
     7741 aatcaatatc agcatgtttt aatatttcca tgatgccgag cggtgtgcaa ggtacaaaag
     7801 tttgttcgtc gatatataat ttccctatat ttattggatg gaaaccgtcc acatctttat
     7861 caggattgat tgcttctaat attttctgtt cgctaacttg ttttggtagt ggtacttgta
     7921 ccaaaatacc acttacagaa tcatcattat ttagtctgtt tagttcgttt aatacttctt
     7981 cttcagtagc tgtttcttcc aaatgtacga tttctgaaat cataccaatt ttttcagctg
     8041 ctttcttttt tgatctaaca taactttgac tagcgccatc attaccaact aatataacgg
     8101 ataatttagg tgtaaaaccc ttttctttta gcgcttcaac ttgattttgt aacccctgtc
     8161 tgtagtcttt ggcaatttgt ttaccatcta aaattttagc aaccataaaa acttctcctc
     8221 ctattgtcaa tatcctaact ttgttagttt ataaaactaa tttacatagc aaaagacgta
     8281 aattcaaaca aaaaatcaaa ataaagttcg atttttgatt gaaaaagcag aaattgcttg
     8341 ttatgctata tctataatat acaactaaag taattcacaa ttcaaacttt tgaaagggtg
     8401 tacaaagtga aagtagcagt cattatgggc agttcttccg attggaaaat tatgcaagag
     8461 agttgtaaca tgttggatta ttttgaaatt ccgtacgaaa aacaagtagt atccgcacat
     8521 cgtacgccaa aaacgatggt tcaatttgct tctgaagcga gagaaagagg tatagacatt
     8581 atcattgcag gcgctggcgg tgcggcacat ttaccaggta tggttgcatc attaacgacg
     8641 ctaccagtta ttggagtgcc gattgaaaca aaaagtttaa agggtataga ttctttatta
     8701 tcaattgttc aaatgccagg aggtattccg gttgcaacga ctgcaattgg tgcagcaggt
     8761 gctaaaaatg caggtatact tgcagcaaga atgttaagta ttcaaaatcc ttctttagtt
     8821 gaaaaactaa atcagtatga atcttcgtta attcaaaaag tggaggacat gcaaaatgaa
     8881 cttcaataaa ttaaagtttg gtgcgactat tggcattatt ggtggtggtc agcttggaaa
     8941 gatgatggca caatcagctc aaaaaatggg ttataaagtg gctgtattgg atcctgctga
     9001 agattgtcca tgtagatacg ttgcacacga atttatacaa gccaagtatg acgatgaaaa
     9061 ggcactcaat caattaggac aaaaatgtga tgtgattact tatgaatttg aaaacatttc
     9121 agcccaacaa ttaaaactat tatgtgaaaa gtacaatatt ccgcaaggtt accaagctat
     9181 acagttatta caagaccgct taactgaaaa agaaacatta aaaagtgctg gtaccaaggt
     9241 tgtcccattc atttcagtaa aagaatctaa agatattgac aaagcaattg aaacattagg
     9301 atatcctttt attgtaaaaa ctagatttgg tggctacgat ggcaaaggtc aagttttaat
     9361 taacaacgaa aaagacttac aagaaggtat taaattaatt gaaactagtg aatgcgtagc
     9421 tgaaaaatat ttgaatatca agaaagaagt atcacttact gttacaagag gaaacaacaa
     9481 tcaaatcact tatttcccat tacaagaaaa tgagcataga aatcaaatac ttttcaaaac
     9541 aattgttcca gcgagaatag ataaaatagc tgaggcgaaa gagcaagtta ataaaattat
     9601 ccaatcgatt catttcattg gaacatttac agttgaattt tttatagata gtaataacca
     9661 attgtatgtg aacgagattg caccaaggcc tcacaattcc ggacattatt caattgaggc
     9721 atgtgattat tcacaatttg atactcatat tttagcagtt accggacaat cattaccaaa
     9781 ttcaattgaa ttattaaagc ctgcagtcat gatgaactta ctaggtaaag acttagattt
     9841 attggaaaat gaatttaatg aacatccaga gtggcactta catatttatg gtaagtctga
     9901 gcgtaaagat agcagaaaaa tggggcatat gactgtacta acgaatgatg taaaccaaac
     9961 tgaacaagat atgtacgcta aatttgaggg ggagtaatta aagcatgaca ttactatatg
    10021 aaggaaaagc gaagcgcatt ttctcaacaa atcaagaaaa tgaattaaga gttgaatata
    10081 aagatgaagt tactgctgga aacggggcta agaaagacac aatggcaggt aaggggcgat
    10141 taaataatca aattacttct attatattta aatatttaca agaaaatgga atagaaagtc
    10201 actttattaa gcaattatct gaaacagaac aattagttaa gcctgtgaaa ataattccat
    10261 tagaagtagt tgttcgtaat attgctagcg gatctattac aaagcgttta ggttttgaaa
    10321 atggtgaagt ttttagagaa ccacttgtgg aatttttcta taaaaatgat gcattaaatg
    10381 atcctttgat tacagatgat cacatcaaat tactgaatat tgcgacagat aaagatattg
    10441 aaacattaaa aacgaaagcg ctagaaatta atcaagtatt aaaacaatta atgcatgcga
    10501 tgaatttaaa attagtagat tttaaaatcg aatttggaaa gactgagact ggtcaaattt
    10561 tgttagcgga tgaaatatct ccagatacat gtcgaatttg ggataaagct accaatgcaa
    10621 actttgataa agatgtatat agaaataaca ctggatcact gattgaaaca tatcaaatat
    10681 ttttaaacaa attggaggat ttaaaataat gaaaacaatt gaactacata tcacattaca
    10741 accacaagta ttagatacgc aaggacaaac gcttactcga gctgtacatg atttaggtta
    10801 tgcacaagtg aatgatattc gtgtaggaaa agtattatat atgacagtgg atgaggttag
    10861 tgatgaaaag gtacacaaca ttattacaac tctaagtgaa aaattgtttg caaatacagt
    10921 gattgaagaa tatagctata aagttttaga agatgaaaag gagaatgcat aaaatgaaat
    10981 ttgcggttct tgtttttcca ggttcgaatt gtgatagaga catgtttaat gctgctatta
    11041 aaagtggtgt tgaagcggaa tatgtagatt atagagaaac atcactaagt ggatttgatg
    11101 gagtacttat tcctggtgga ttttcattcg gggattactt aagatctggg gcaatggcta
    11161 gtgtagcgcc ggttatttct gaagttaaac gtcttgcagc tgaaggtaag ccagtattag
    11221 gtgtttgtaa tgggtttcaa attttaactg aaataggctt attacctggt gcattattgc
    11281 ataacgattc acatttattt attagtagaa atgaagagtt agaaatagtg aataatcaaa
    11341 cggcttttac aaatctttat gaacaaggtg aaaaagttat atatcctgta gctcacggcg
    11401 aaggtcatta ttattgtact gatgaaatat atcaaaaatt aaaagctaac aatcaaatta
    11461 ttctgaaata tgtgaataat ccgaacggtt catatgacga tattgcagga attgttaacg
    11521 aaaaaggcaa tgtgtgtggc atgatgccac atcctgaaag agctttagaa acgttgttag
    11581 gtactgatag tggtgtgaaa ttatttgaag cgatggtaaa aagttggagg gaacaacatg
    11641 tctaaattta tcgaaccaag cgttgaagaa attaaacttg aaaaagtata tcaagatatg
    11701 ggattaagtg atcaagaata tgaaaaagtt tgcgatattt taggcagaca acctaacttt
    11761 acagaaacag gtatcttttc tgttatgtgg agtgaacatt gctcttataa acattctaaa
    11821 ccgtttttaa aacaatttcc tacgtcaggt gagcatgtgc ttatgggtcc aggtgaaggt
    11881 gcaggggtag tcgatatagg tgataatcaa gccgtagtat ttaaagtaga atctcacaat
    11941 catccatcag caattgagcc atatcaaggt gctgctacag gcgttggtgg aatcattcgt
    12001 gacattgtct ctattggggc tagacctatt aatttgttaa acagtcttag atttggagaa
    12061 ttagataata aacaaaacca aagattactt aaaggtgttg taaagggtat cggaggttat
    12121 ggtaactgca ttggtattcc aacaactgct ggagaaatcg aatttgatga acgttatgat
    12181 ggtaatccac ttgttaatgc aatgtgtgtt ggtgttatca atcacgacat gattcaaaaa
    12241 ggcacagcaa aaggtatagg taattcggtc atttatgttg gtttgaaaac tggtcgagat
    12301 ggtattcatg gtgctacttt tgcatctgaa gaattgacgg aagaaagcga aagtaaacga
    12361 ccttctgtac aaatcggtga tccatttgta ggtaaaaaat taatggaagc aacacttgaa
    12421 gcaattacat ttgatgaatt agttggtatt caagatatgg gtgctgctgg tttaacatct
    12481 tcatcttctg aaatggcggc aaaaggtggt agtggtttac atttgagatt agaacaagtg
    12541 ccaacacgtg agccaggtat ttctccttac gaaatgatgc tttcagaaac tcaagaacgt
    12601 atgttactag ttgttgaaaa aggtactgaa caaaaattct tagatttatt tgataagcac
    12661 gaattggata gtgctgttat aggtgaagta acagatacaa atcgttttgt tttaacatat
    12721 gaagacgaag tttatgctga cattccagtt gaaccactag ctgatgaagc acctgtatat
    12781 attttagaag gagaagaaaa aaattataat acttctaaaa atgattatac acacatcgat
    12841 gttaaagata ctttctttaa attacttaag catccgacta tagcatctaa acactattta
    12901 tatgatcaat acgatcaaca agttggtgcc aatacgataa ttaagccagg acttcaagca
    12961 tcggtagtac gtgtggaagg cacaaataag gcaattgctt caacaattga tggtgaagcg
    13021 ctttatgtat ataacaatcc atatgaaggt ggaaagatgg tagtagctga agcttatcgt
    13081 aatttaattg ccgtgggtgc aacaccatta gcaatgactg actgcttaaa ttatggttct
    13141 cctgaaaaga aagaaattta tcaacaatta gcagattcaa cgaaaggtat ggcagaagct
    13201 tgcgacatac ttaagacacc tgttgtttct ggaaacgttt ctttatacaa cgaaacgaaa
    13261 ggcacttcta ttttccctac accaattgtt ggtatggtag gtctgattga aaacgtagac
    13321 tatttaaatg attttcaacc tcaagttgga gataagttat atgtaattgg tgatactaag
    13381 gacgactttg gtggtagtca acttgaaaaa ttgatttatg gcaaagtaaa tcacgaattt
    13441 gaatctatag atttgagttc agaagttaaa aaaggtgaat caatcaagac cgctattcgt
    13501 caaggggtat tatcacatgt tcaaacggtt ggtaaaggtg gcttactgat taccttagct
    13561 aaactaagtg cgcattacgg tttaggatta aagtctttaa tagatataac aaatgcacaa
    13621 ttgtttagtg agacacaagg ccgatatgtt gtttctgtta aatcaggtaa aactttaaat
    13681 attgataatg caatagaaat tgggctttta acagatagtg ataatttcaa agtaacaaca
    13741 ccatatacag agattagtga aaatgtttca gatattaaac aaatatggga aggggcaatt
    13801 gctcaatgtt taactactca ggattaaacg aagaatgtgg cgtgtttggt atttggaatc
    13861 atcctgaagc agcgcaacta acatatatgg gacttcatag tttgcaacat cgtggtcaag
    13921 aaggtgcagg tatagttgtt tctgatcaaa atgaattaaa aggcgagcga ggattaggct
    13981 tactaactga agcgattaaa gatgaccaaa tggaacgatt aaaaggatat caacatgcaa
    14041 ttggtcacgt ccgttatgct acttcaggta ataaaggtat tgaaaatatt caaccgcttc
    14101 tgtatcactt ttatgatatg agtgtaggta tttgtcataa tggtaacctc attaatgcta
    14161 aatcattacg tcaaaattta gaaaaacaag gtgctatctt ccattcgtct tcagatacag
    14221 aagttattat gcatttgatt cgacgcagta aggcgccaac ttttgaagaa gcattaaaag
    14281 aaagtcttcg aaaaattaaa ggtggtttta catttgcaat tttaacaaag gatgcgttat
    14341 atggcgcagt agatccaaat gatatcagac cacttgttgt aggtaaaatg aaagatggga
    14401 catacatcct tgcaagtgaa acatgtgcaa ttgatgtgtt aggtgcagaa ttcgttcaag
    14461 atattcatgc gggtgaatat gtcgtgatta atgataaagg tattacagtt aaatcttata
    14521 cacatcatac gacaactgca atttctgcga tggaatatat ttattttgcg agaccagatt
    14581 caacgattgc tggtaaaaat gttcatgcag tgcgtaaggc ttcaggtaaa atgttagcac
    14641 aagaaagtcc ggtaaaagct gatatggtca tcggtgtacc taattcatca ttatctgcag
    14701 cgagcggtta tgctgaagaa atcggtttgc catatgaaat gggactagtt aaaaatcaat
    14761 atgttgcaag aacatttatt caaccaacgc aagagttacg tgaacaaggt gtaagagtta
    14821 agctatctgc agtgaaagat attgtagacg gtaaaaatat tattcttgtt gatgattcca
    14881 ttgttcgagg tacgacaatt cgacgtattg tgaaaatgtt aaaagattct ggtgcaaata
    14941 aagtgcatgt gcgtatagca tcaccggaat ttatgttccc aagtttttat ggaatcgatg
    15001 tttcaactac ggcagaatta atttctgcaa gcaaatcacc tgaagaaatt aaagattata
    15061 ttggcgctga ttcgttagca tatctatctg tagatgggtt aattgaatca attggtttag
    15121 attatgacgc gccatatagt ggcttatgtg tagaaagttt cactggagat tatcctgcag
    15181 ggttatatga ttatgaagca aattataaag cacatttaag tcatcgacaa aagcaatata
    15241 tttctaaaaa caaacactat tttgatagcg agggaaattt aaatgtctaa agcatatgaa
    15301 caatctggtg taaatattca tgctggttat gaagctgtag aaagaatgtc tagtcatgtt
    15361 aaacgtacga tgcgtaaaga agttatcggt ggtttaggtg gattcggtgc tacatttgat
    15421 ttatcacaat taaatatgac agcaccagtt ttagtttctg gaacagacgg agtaggtacg
    15481 aaattaaaac tagctatcga ctatggtaaa catgattcga taggtatcga tgcagtcgca
    15541 atgtgtgtta atgatatttt aacgacaggt gcagaaccat tatatttttt agattacatt
    15601 gctacaaaca aagttgttcc tgaagttatt gaacaaattg ttaaaggtat tagtgatgca
    15661 tgcgttgaaa cgaatactgc acttatcggt ggagagactg ctgaaatggg tgaaatgtat
    15721 cacgaaggcg aatatgatgt agctggattt gctgttggag cagttgaaaa ggatgactat
    15781 gtagatggtt cagaagtgaa agagggacaa gttgttatag ggcttgcgtc aagtggcatt
    15841 cattcaaatg gatacagttt agtgcgcaaa ttaattaatg aatcaggcat tgatttggca
    15901 tcaaacttcg ataatcgtcc atttatagat gtctttttag aaccaactaa attatatgtt
    15961 aaacctgtac ttgctttgaa aaaagaagtt tctattaagg caatgaatca tattactggt
    16021 ggaggttttt atgaaaatat tccacgtgca ttgccagccg gatatgctgc tagaattgat
    16081 actacatcat ttccaacgcc aaaaatattt gattggttac aacaacaagg caatatagag
    16141 acaaatgaaa tgtataacat ttttaacatg ggtattggct atacggttat cgttgatgaa
    16201 aaagatgcac cacgtgcttt gaagatttta gcagaacaaa atgtggaagc ctatcaaatt
    16261 ggtcatattg tgaaaaatga gtcaactgca attgaattgt tgggggtata acaatggtta
    16321 aaattgcgat ttttgcatca ggttcaggaa gtaactttga aaatatagtt gagcatgttg
    16381 aatcaggaaa acttgaaaat attgaagtta cggcgctata tacggatcat caaaatgcgt
    16441 tttgtataga tagagcaaaa aagcacgata ttcctgttta tattaatgaa ccaaaacaat
    16501 ttggttcaaa agcagcgtat gaacaacatt tagtaacatt attaaatgaa gataaggtag
    16561 agtggattat tctagctggc tacatgcgtc taattggtcc agatttatta gcttcatttg
    16621 aaggtaaaat attaaatata catccatcac tattgccgaa atataagggg attgacgcaa
    16681 taggccaagc atatcatagt ggcgatacta ttactggttc gacagtacat tatgttgata
    16741 gtggtatgga tacgggagaa attattgaac agagacaatg tgatattaga ccggacgatt
    16801 caaaagaaca attagaagag aaagtaaaaa aattggaata tgagttatat ccaagtgtta
    16861 ttgctaaaat tgtaaaataa ggagtatgta tgtgatgaag aaagctattt tgagcgtatc
    16921 aaataaaaca ggtattgtag agtttgctaa agcgttaacg caattaaatt atgaattata
    16981 ttcaactggt ggtactaagc gtatattaga tgaagcaaat gtaccagttc gttctgtttc
    17041 agacttaaca cattttccag aaataatgga tggccgtgtt aaaacattac atccagctgt
    17101 tcatggtggt attttggctg atcgaaataa accgcagcat ttaaatgaat tatcagaaca
    17161 acatatagat ttaattgata tggtagtagt taatttatat ccattccaac aaactgttgc
    17221 taaccctgat gtgacgatgg acgaagcaat tgaaaatatt gatattggtg gtccaacaat
    17281 gttacgtgct gcagctaaaa actataaaca tgtaacaaca attgtacatc ctgcagatta
    17341 tcaagaagta ttgacgcgat taagaaacga ttcgttagat gagtcatata gacaatcatt
    17401 aatgatcaaa gtttttgagc atactgcaga atatgatgaa gcgattgtac gtttctttaa
    17461 aggggataaa gaaactttaa gatatggaga aaatccacaa caatcagcgt attttgtgag
    17521 aacttcgaat gcaaagcaca cgattgcagg cgctaaacaa ttacatggga aacaattaag
    17581 ctataacaat attaaagatg cagatgctac actagcttta gttaaaaagt ttgatacacc
    17641 tgctgcagtt gcagttaaac acatgaatcc atgtggtgtt ggtatcggtg acacgttaga
    17701 acaagcattt catcatgcgt atgaagcgga tagtcaatca atatttggtg gaattgttgc
    17761 attaaaccga gctgtaacac ctgagttagc agagcaattg catagtatct ttttggaagt
    17821 cattattgca ccaaaattta cagatgaagc attaaatatt ttaaaacaaa agaagaatgt
    17881 aagattatta gaaattgata tgactataga cagtaacgaa gaagagtttg tttcagtatc
    17941 tggcggatat ttagttcaag ataaagacaa ttatgtcgtg ccaaaagaag aaatgaaagt
    18001 tgttacagaa gttgcaccta ctgatgaaca atgggaagca atgttattag gatggaaagt
    18061 tgtaccatca gtaaaaagta atgcaattat tttaagtaat aataaacaaa ctgtaggtat
    18121 aggtgctgga caaatgaatc gtgtcggtgc tgctaaaatt gcgttagaga gagctattga
    18181 aatcaatgat catgtagcgt tagtatctga tggatttttc cctatgggag atacagttga
    18241 acttgcagca caacatggta taaaggcaat tatccaaccg ggtggttcga ttaaagatca
    18301 agattcaatt gatatggcta ataaacatgg tattgcaatg gtagtcacag gcactcgaca
    18361 ttttaaacac taattttaaa ggggcgtaac ataaatgaat gtattagtaa ttggtgctgg
    18421 tggacgagaa catgcacttg catataaact taatcaatcg aatctagtta aacaagtgtt
    18481 tgtcattcca ggtaatgagg caatgacacc tatagctgaa gtacacactg aaatttcaga
    18541 atctgatcat caagggatac tagattttgc taaacagcaa aatgttgatt gggtagttat
    18601 aggtccagaa caaccgctaa ttgatggatt agcagacatt ttacgagcga atggtttcaa
    18661 agtgtttggt ccaaataagc aagcagctca aatcgaaggc tcaaagttat ttgctaaaaa
    18721 gataatggaa aaatataata ttccaactgc tgattataag gaagtagagc gaaaaaagga
    18781 tgcattaaca tatattgaaa actgtgaatt ccctgttgtt gtcaagaaag atgggttagc
    18841 tgctgggaaa ggcgttatta ttgcagatac aattgaagca gccagaagtg ctattgagat
    18901 tatgtatggt gatgaagaag aaggtactgt tgtgttcgaa acgtttttag aaggtgaaga
    18961 gttctcgcta atgacatttg ttaatggtga attagcagtt cctttcgact gtattgcaca
    19021 agatcataaa cgcgcatttg atcatgatga aggaccaaat actggtggta tgggggctta
    19081 ttgtccagta ccacatatta gtgacgatgt tttaaaactt acaaatgaaa caattgcaca
    19141 acccattgca aaggcaatgc ttaatgaagg ttatcaattc ttcggtgtat tatacattgg
    19201 tgctattcta actaaagatg gtccaaaagt aatagaattt aatgcccgtt ttggtgatcc
    19261 tgaagctcaa gtattattaa gtcgcatgga aagtgattta atgcagcata ttattgattt
    19321 agatgaagga aaacgtactg aattcaaatg gaaaaatgaa gctattgtag gggtcatgtt
    19381 ggcatcaaaa ggatatcctg atgcatatga aaaagggcat aaagtaagtg gctttgattt
    19441 aaatgaaaac tattttgtta gtggattaaa gaagcaaggt gatacctttg ttacttcagg
    19501 tggtagagtt atacttgcca tcggaaaagg tgacaatgta catgatgcac agcgagacgc
    19561 atacgaaaaa gtatctcaaa tacaaagtga ccatttattc tatcgtcatg acattgcgaa
    19621 taaagcacta caacttaaat aagtatattt aaaaatacta agattagcta tgaacgaatc
    19681 tataacgata gatttgttca tagctttttt agttgtagag tctaggacat tgatttctgt
    19741 accaaatttg tgattatcat atgttgtaat acaaaagagg cgccacaaca tgtttggatg
    19801 aacaaaataa catgtttgtg gcacctcttt tgtttagtat ggaataaatg gttttctttt
    19861 tctatacaat gaatttctaa tttagtatct atacaattat ggataaaatt taacctacac
    19921 gaccaagacg aacatcatca atgcccgtga tgggtaaggt gattgaacaa taatatgcca
    19981 aaataataat ggcaattaaa actataataa agattacatc tttatatgag aaaggtacgt
    20041 tgtaatagta agtacgagga ccatctctaa atcctttcga ctccatcgca actgataatt
    20101 gatgtgcctt tctaatgttt tgacttaata gaggtatgat taaatgctta aatcgcttta
    20161 atcctctata atttgctgcg tctatcatct gatagcgcat ttttaaagat ctgcgaagct
    20221 gtattaaaga actaatcatt aaaggtatca tacgaatggc agccatgaat gcataagcaa
    20281 cttttgattt aacctttaaa tgttgcatta aactataaaa aatcatgaca acctgagatg
    20341 tgagtgcgat taagataccg aaaaatgaaa tagcaatagt tcttaatgat acatgtaaac
    20401 cacgaactaa actttctgtt gtaatatgga taaatccgaa tttcaaaatt gtatggctac
    20461 cattcccgta taaaatcatg aacagggaag agagtaatgc aaagccaata cttatagtta
    20521 taaaaattgc tgtaatttta aactgagtac cattaaacat caataagaaa actaacatta
    20581 agatagtgat ataaagcata aaatcgaaat tatgcacaaa tataataaag aaaaatagta
    20641 taattccaag aaatagtttc gttataatgt tgacatcatc aacaaatgat tgccgaacct
    20701 tccattgctc atacattcgt atcaccatca caatctagta acgcaccatc tgaaatttta
    20761 agtcttcttg atgggtaacg ttcaattatt tcatcgtcat gtgtaaccat gacaatactt
    20821 tgtcccaaat taattcgttt ttggaaaagt ttgatcaact ggaatgtatt atggctatca
    20881 agtccaaatg tcggttcatc taaaaagatg atatcagctt tagaacttag tgcggtagct
    20941 acgctaaggc gtcgtttttg accaatagac aactcataag gatgttgatc tttaacattt
    21001 tctaaatcta aaagttttaa aagttgtatc gtttcatcat cactttgatc tttagaaagg
    21061 tgattaaaat gaatgttaat ttcatcataa accgaatttg ttataaattg taactctggg
    21121 ttttgataaa ctagatacat gtgttttgct gcatgtttaa tttttgttaa atgctgattt
    21181 tcaaaataaa catcaccttg atatttaatc aattgcataa ttgattcaag caaggttgtt
    21241 ttaccactac catttgcccc tgtaattgta atccactcac ctagaccaat ttctaaatct
    21301 gagaatgaga gcaacgttga tttaccgcga ataatacgtc catttttaaa ttgtaataag
    21361 tgtgagtttg ttgttggaaa gtcaacacga cttggtgcga attcccatgc acgtggatgc
    21421 cacacaccat attcactgag taaatgaaca tacttctgta atatgatttc aggacattca
    21481 tcggcaatga tatttccgtt ataatccatc aaaatgacgc ggtcgacatg attccagatg
    21541 tgtttaactt tatgttcaac gattacaaca gtttgatctt cccaaagttc aattagttta
    21601 gtccataaat cttccgttgc ttgaacatct aacattgctg tcggttcatc taaaaacaat
    21661 gtttttgatt gttgaagaat ggtttcaaca attgccaatt tctgtttcat cccgccactt
    21721 aaatctttga tatacgtttc aggggtaaca tttaaattga ccatatttaa agcattgata
    21781 attaacgcat ccatgtcttc acgtggtact tgtctatttt ctaaaacgaa tgcaagttct
    21841 tcgtatactt ttggcataca aaactggcta tcagggtctt ggaaaataac gccacttaat
    21901 gggtcaacga ttagttcatc atatttcata ggtaattcaa ttaaattagg aacaatacca
    21961 cttaatacat tcagaagtgt acttttaccg caaccagaag gaccgagtaa aagtactttt
    22021 tctttgtctt gaatagtgat atttaaatga tcgaaaactt tacgctgacc acttggatat
    22081 tttaatcgta aatcacttac ttttaacact ctgaatgctc ctcttataag ttgtcgtaat
    22141 cttctttagc agctggccta aataattttg ttacgcctgt cttatctaaa gcttttacta
    22201 aaaggtaaga taggacgccg gcgactactg caccactaat taatctaaat acgatgaata
    22261 atgttaagtt ccaacctgca acttcattta aataaccata gaaataatcg atcgggaaag
    22321 ccgcgatagc tgtacaaaaa cctgctaaca tagctaccat aactgaacga gattgatatt
    22381 taaaaattgc aaagacaagt tcacacgcta aaccttgtat gaaagcgtaa acgattgtcg
    22441 gaatatcgaa acgacccata atgatagttt cgccggcacc tgcagcaaat tcagccaata
    22501 aagcaatacc tggttttgga ataattagat agcagacaat cgctgccatg aaccaaaccc
    22561 cgtttgttaa ttgttcgagg tgaaggcctg tagcttgcac accattgtaa acaaaccacc
    22621 ataaattgta aataactgcg aatactactg aaataagtac ggttactaga atttcagata
    22681 gctttaaacc ttttgacatt tttacatcct cctaataaaa aaacgcacaa ccatccatag
    22741 gaaagttatg cgttcacaat atatattagt aaaacatatg tatagtaaca ctttcctacg
    22801 ctagttcaag ctagatcagg ttcaaagggt ttgagggcaa gcctcatctc agtataaaac
    22861 acccctagtg tgtgcgattt atttaattaa ttatactgta agacgtttat aaacttatgt
    22921 caataggttg tcttcatgaa atttcgttta attcgattta aaatttataa tattagcatt
    22981 agatttaaat taaagatgta gtagaaatgt tagtaattaa agatataaaa atatgtgaca
    23041 tgtaataata ttaagttgat aagtgaagag ggatacttat caatcatacc tctttaacaa
    23101 cagtgaacaa cccgtgcata atggcttgtg aattattgtt tgtaaggtaa aagagggata
    23161 ccagcggcga gcgcaagcaa aaaagcccca aacaactaaa agttataagg taaaagaggg
    23221 atacttgcgc cgagcgcatg cataaaatcc cctaacaacg aaaagttgta aggggattta
    23281 aattaattta gtgtatcttg gatatcttgt tttgtttgat taatatcttc tgttttttct
    23341 tcttttttat cttttagttt ttcttcaact tctttagctt tttctgctgc ttttttattt
    23401 tgattttcat ttgacatgat taattcctcc caaattggat aattatttat atataaatct
    23461 tacccggttg tactttcgtt aaacttttct aagtctatag cactatttat tcatttatct
    23521 aaagacaaca acattagatt aatatataat gattttgagg tgaacataat gtcttttctt
    23581 aggaaacacg ccgaaattat ttttagctat ttaatcggta tcgtttcact cttcactggt
    23641 ctcattattt taattaactt gcctttaatt aaacaattaa atggtggtaa aaaagttgat
    23701 acacatgttc ataatgtgtg ggaatttctg aatgcatttt tcagtgaaat tattaaagta
    23761 atgagtcgat ttataggtaa tttccctata gttagtgcaa ttgtgataat tatattcggt
    23821 attttagtta tgttgattgg tcatacatta cttagaacta ttaagtatga ctatgatatt
    23881 tctatctttt tcttagttat cggtatcatg tactttatta ttacgcttat attaatgacg
    23941 caagtatatg gattctttgc ggtaattttc attattccat ttacaattca tattggatat
    24001 atcgtttata aagatgaatt gaat
//
LOCUS       DAKSQE010000030        22979 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.030, whole genome shotgun sequence.
ACCESSION   DAKSQE010000030 DAKSQE010000000
VERSION     DAKSQE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22979
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.030"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            41..217
                     /locus_tag="O0V33_001050"
     CDS             41..217
                     /locus_tag="O0V33_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975697.1"
                     /translation="MKHLTKIFVALAIILFIVGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSNDRRKNNK"
     gene            475..855
                     /locus_tag="O0V33_001051"
     CDS             475..855
                     /locus_tag="O0V33_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCX8975698.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            852..1748
                     /gene="pmtA"
                     /locus_tag="O0V33_001052"
     CDS             852..1748
                     /gene="pmtA"
                     /locus_tag="O0V33_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HCX8975699.1"
                     /translation="MNAIEVSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNRIGFVYAENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGAIEDLDDELASLLIFEEHKRTGFIGLTEHAQVFKEILGHKVNITTPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
     gene            1749..2429
                     /gene="pmtB"
                     /locus_tag="O0V33_001053"
     CDS             1749..2429
                     /gene="pmtB"
                     /locus_tag="O0V33_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HCX8975700.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLVGT
                     LVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLVNYFVLSQSSFPDLYSYILNIGFLIISIVILIVNYFKQLNKINT
                     RKFKGGSR"
     gene            2426..3298
                     /gene="pmtC"
                     /locus_tag="O0V33_001054"
     CDS             2426..3298
                     /gene="pmtC"
                     /locus_tag="O0V33_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HCX8975701.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
     gene            3298..4038
                     /gene="pmtD"
                     /locus_tag="O0V33_001055"
     CDS             3298..4038
                     /gene="pmtD"
                     /locus_tag="O0V33_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HCX8975702.1"
                     /translation="MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLA
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGDKVLDAFDYIPFAYLTDKMISSNFDF
                     SNWQWVISLGSIVIFFILNILYVAKKDI"
     gene            complement(4102..4665)
                     /locus_tag="O0V33_001056"
     CDS             complement(4102..4665)
                     /locus_tag="O0V33_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCX8975703.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKLENK"
     gene            4786..5310
                     /locus_tag="O0V33_001057"
     CDS             4786..5310
                     /locus_tag="O0V33_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975704.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDLEIKRIEFFSK"
     gene            5370..5927
                     /locus_tag="O0V33_001058"
     CDS             5370..5927
                     /locus_tag="O0V33_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCX8975705.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEE
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKI
                     LEYIYRLILKYLLWYIKTVKGSVRK"
     gene            5924..6766
                     /locus_tag="O0V33_001059"
     CDS             5924..6766
                     /locus_tag="O0V33_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCX8975706.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNISSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSPTYISKSNIKNSNIKVVIGMLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDVRFKYDSKPEDTILKLNPGTGDSVVKNKTFINGKVGKSDSVLEFYTIDGN
                     IKVE"
     gene            complement(6823..7863)
                     /locus_tag="O0V33_001060"
     CDS             complement(6823..7863)
                     /locus_tag="O0V33_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCX8975707.1"
                     /translation="MQQVTSDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYHPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
     gene            8314..8487
                     /locus_tag="O0V33_001061"
     CDS             8314..8487
                     /locus_tag="O0V33_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975708.1"
                     /translation="MWNFIKCVFKFVFSLVAITTLVAGVGVVAFAYIFKKDFEDIERK
                     TKEIISDIESKNN"
     gene            complement(8544..8945)
                     /locus_tag="O0V33_001062"
     CDS             complement(8544..8945)
                     /locus_tag="O0V33_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HCX8975709.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFATLPEQFETI
                     QQYIIDQNKITRPVLSDDQLAELNIRLHEALQYAQPVEVKFYNNGFVDSVRLTIYRID
                     AINYEIDGYVYNQQQRQKISIFDILDIVLLP"
     gene            9216..10244
                     /locus_tag="O0V33_001063"
     CDS             9216..10244
                     /locus_tag="O0V33_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCX8975710.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERNKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            complement(10364..11743)
                     /locus_tag="O0V33_001064"
     CDS             complement(10364..11743)
                     /locus_tag="O0V33_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCX8975711.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKNARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTNDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            11795..11968
                     /locus_tag="O0V33_001065"
     CDS             11795..11968
                     /locus_tag="O0V33_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975712.1"
                     /translation="MNVVAVNFKWSFELYMLKVENRAMNRVHNNIYNFNHNEKGRKKT
                     KIYTQHRKYFKKM"
     gene            complement(12161..13090)
                     /locus_tag="O0V33_001066"
     CDS             complement(12161..13090)
                     /locus_tag="O0V33_001066"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCX8975713.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(13143..13703)
                     /locus_tag="O0V33_001067"
     CDS             complement(13143..13703)
                     /locus_tag="O0V33_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCX8975714.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            14076..15173
                     /locus_tag="O0V33_001068"
     CDS             14076..15173
                     /locus_tag="O0V33_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HCX8975715.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQRALNHGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGHRFGFYRGYNGHSHIHIKGGKFDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKNCSFEGFVDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVIIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENINIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNTFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINVDEIKK"
     gene            15378..16940
                     /locus_tag="O0V33_001069"
     CDS             15378..16940
                     /locus_tag="O0V33_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCX8975716.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSTGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGHEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYIMPIV
                     LGIDINQPLPLK"
     gene            complement(16951..17058)
                     /locus_tag="O0V33_001070"
     CDS             complement(16951..17058)
                     /locus_tag="O0V33_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975717.1"
                     /translation="MVDFLLFVLFLHDATVYVSLPLVQAIANVTFYFSF"
     gene            complement(17130..17924)
                     /locus_tag="O0V33_001071"
     CDS             complement(17130..17924)
                     /locus_tag="O0V33_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCX8975718.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNLFEVIKSVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGSNTISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            complement(17944..19020)
                     /locus_tag="O0V33_001072"
     CDS             complement(17944..19020)
                     /locus_tag="O0V33_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HCX8975719.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSINYHIAQATNEGKL
                     KPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKYYEYPTSLIKEVPIEHDHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKKSNANQCPFHH"
     gene            19204..20673
                     /locus_tag="O0V33_001073"
     CDS             19204..20673
                     /locus_tag="O0V33_001073"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCX8975720.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVYTYKMKFIKSFEAIDLHHNIYENGKLVYQIPTEDESRDYLAQGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            20666..21487
                     /gene="nadE"
                     /locus_tag="O0V33_001074"
     CDS             20666..21487
                     /gene="nadE"
                     /locus_tag="O0V33_001074"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCX8975721.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIAELKQFIKSYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVEQALQFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKQLYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            21757..22359
                     /locus_tag="O0V33_001075"
     CDS             21757..22359
                     /locus_tag="O0V33_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCX8975722.1"
                     /translation="MSFVTENPWLMVLTIFIINVCYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            22340..22513
                     /locus_tag="O0V33_001076"
     CDS             22340..22513
                     /locus_tag="O0V33_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HCX8975723.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQKDGTVEV
                     SHQEIIFVGKKIQ"
     gene            complement(22807..>22979)
                     /locus_tag="O0V33_001077"
     CDS             complement(22807..>22979)
                     /locus_tag="O0V33_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HCX8975724.1"
                     /translation="ICKYINTEHKQLTLYNKNNSSIVIEIFIPNDNKILLTIMNTEAL
                     GTSPRMTFIKHK"
BASE COUNT         7894 a         3187 c         4019 g         7879 t
ORIGIN      
        1 ctttatattt gttataatgt cgatgttgag gtgaaataaa ttgaaacatc taacaaaaat
       61 atttgtagca ttagcaatta ttttatttat tgttggttat tatttacaag caacaaacca
      121 tgagagccaa ggtataaaac tattattggc agcaattatg tttatgattt gtgcttttat
      181 aagtagaagt aatgatcgac gtaaaaataa caaatagaat tgactgtttt aaacgactgt
      241 tcttaatgac gcgacaatca ccgtcaaagt ataagaatat tcgttttgac agtctttttt
      301 attgaagtag acaatgcaag attaaatggt agagtcattt cattttattc aactgctagt
      361 aatacaatac ccagtgttat ttctgaagac tggttgataa tgaaacatgc atatttacaa
      421 aactgtgtat attgtgtata ttgtatatat acagaaggtg atagggggac gttgatgaaa
      481 ataattttaa aaaacaatag tgattttccg atttatgaac agattaagca acaagtaaaa
      541 caaaatattt taaagggaca tgttgctcct ggagagcatt tgccatcaat gagggaactt
      601 gccaaagatc ttcaagtaag tttaattact accaaacgtg cttatgaaga tttagagaaa
      661 gatgggtttg ttaccacaat tagaggaaaa gggacctttg ttaaggagca agatagttct
      721 attttaaaag agaaacaatt ttttaccatt gaaaatttgg ttaaagaatt ggttaatgaa
      781 gcgcaagcca tcgaaatgtc acttgaggaa cttcaagata ttttaacgtt catttatgag
      841 gaggaatcat catgaatgcc atagaagtaa gtaatgttaa ttattcaagt gatcagttta
      901 atttaaagaa tatctctttc aaagttcctc aaggattcgt cactggtttt attggtagaa
      961 atggcgctgg taaaaccaca ataataaggt taattatgga tttgtatcaa ccacaaactg
     1021 gtgttattag agtattagaa gaggatatgg ctctcaatcc gatagagtta aagaatagaa
     1081 ttgggtttgt ttacgctgaa aattatttta atgagagatg gactacgaag caacttgaaa
     1141 aaatgattgc ccctttttat cgtaaatggg accatcaagt atttgaattt tatcttgaaa
     1201 agttcgattt accaatcaat aaatcaataa aaacattttc gactggcatg aaaatgaaat
     1261 tatcattagc agttgcattt tcacatcatg ctgaattgta tatttttgat gagcctactt
     1321 caggtcttga tcccttagct agaaatgaac tattggagat aattcagcaa gaattaattg
     1381 atgaaaataa aacaatattc atgtcgacac acattatctc agatttagaa aaaatagctg
     1441 attatatcat tcacttaagt gacggtgaag ttatcttaaa tggttcaaaa gaacaactgt
     1501 tacaaagata tcaagttgtg agtggtgcaa ttgaggacct tgatgatgaa ttagcatctc
     1561 tacttatttt tgaagaacat aagcgtacag gatttatagg attaacagaa catgcgcaag
     1621 tatttaaaga aattttaggt cataaagtga atatcactac accttctatt gaaaacttaa
     1681 tggtttatct tgaaaagaga aaacctaaat atcatgaaaa catcaaattg atggaagaag
     1741 gtttttaaat gaaggcattg ttaattagga attttaaatt acgacgatat acacttatca
     1801 tctatgttct attattaacc ttatatcctt tttatataat gcttgactca acaaagttct
     1861 tttacctatt gcaatctttt ataagtccaa caatattgat tatttggatt ttagatgctg
     1921 gtcacttatt tagactcaat cgtagattag gtggtaatga ttcatattat ttttatatga
     1981 gtttacctgt gtcaaaaaaa caattgctta atgcgaacta tattacatgc attgttttaa
     2041 cattagttgg aacgcttgtt attagtttat atgcttatga agcagatgtg attgaaccaa
     2101 attcaattta tttttcaact gcatatgcat ttgtcatatc taacttcttg tctataccaa
     2161 ttgcatttag tcaatttaca gaattgcgca gagtcaaagt gccctatggt atatacgtgt
     2221 ttactattat cattttagtt ccatttttat tttcaattgc aatagtgttg gtgaattatt
     2281 ttgttctaag tcaatcatca ttcccagatc tatattcata tattttaaat attggttttc
     2341 taattataag cattgttata cttattgtta attattttaa acagcttaat aaaataaata
     2401 ctagaaaatt taaaggaggc agtcgatgaa attagaacat attacaaaaa aatacggctc
     2461 aaatgtcgtt ttaaatgata ttgattttga ctttggcgat agtagaattg ttggattaat
     2521 agggaaaaac ggtgttggta aaacaaccgt tatgaaagta atgaatggta atattattaa
     2581 atttgatgga aaagtagata ttgataatgc agataatatc ggttttttaa ttgagcatcc
     2641 taaattatat gataataaat caggattgta taacttgaaa ttatttgcac aagtattagg
     2701 taagggtttt gataaagcat acacagacaa aattatagat gcatttggta tgagacctta
     2761 tattaaaaag aaagttaaga aatattcaat ggggatgaag caaaagttag caattgcagt
     2821 atctttaatg aataaaccta aatttttaat cttggatgag cctacaaatg gtatggatcc
     2881 agatggctca attgatgtgc tgactacaat taagtctcta gtaaatgaac ttgatatgag
     2941 aattctaata tcaagtcata agttagaaga tattgaatta atttgtgata gagctgtatt
     3001 tttgagagac ggacattttg ttcaagatgt aaacatggag gaaggtgttg catctgacac
     3061 aacgatagtt actgttgatc ataaagactt tgatagaact gaaaaatatc ttgcagagca
     3121 tttccaatta caaaatgtcg acaaagcaga tggacattta atgatcaatg cacaaaaaaa
     3181 ttatcaagtt atactaaaag cattatctga attagatatt tatccgaaat atattgaaac
     3241 acgtaaaagt tcattgcgtg atacgtactt caatataaat caaagaggtg ataaataatg
     3301 agaattttaa atttagttaa gtatgatttt tatagtatat ttaaaagtcc tttaacatat
     3361 ttagcgatac tagtcgtatc tagtttgatt gcaacgcaaa gtatacttat ggcaaattcg
     3421 atggataacc cgaaacatat tattgtctat ggatctgtat ttgctgcagc aaaatggtta
     3481 ttgttaataa ttggattaat gtttgttgtt aagacaatta cgcgtgattt ttcacaaggt
     3541 acaatccaac tatatatgag taaagttaaa acacgtgttg gatacattat ttcgaaaaca
     3601 atttcaatta ttttaatttc aatattattt gcattaattc attatgtgat tttgattgtt
     3661 gtgcaggcat ctagtaatgg gaaaaatttg gcgttttcta aatatgtaga taatttatgg
     3721 ttcttcctaa tctttttact attctttggc ttgtttttat tcttaatcac acttgcatca
     3781 caaaaaacag caatgatatt ttcattaggt gtatttttag tacttattgt accgtttatt
     3841 aaaccattca ttacatttat tccaagatat ggtgataaag ttttagatgc ttttgattat
     3901 atcccttttg cttacttaac tgataaaatg attagctcta actttgattt tagcaattgg
     3961 caatgggtaa tttcattagg ttctatagtg atattcttca ttttgaatat cttatatgtc
     4021 gctaaaaaag acatttaata aaaaattttg aggttgggaa ttttaaattt tcccaacctc
     4081 aaagtttgtc ttattgtaaa tttatttatt ttctaatttg tttaggatgg aattataaac
     4141 tgctttccaa aatgaagcgt cagttttata gcgatttgat ataactaagt gtgtttcttt
     4201 ttctaaatct gcatagtctg gatgatcttt gctcggtaat ttatcggcac gaacatcagt
     4261 tacaaatttt tggacttcat ttgctcttgg tccccaaact gtttcttgtt cgaattgatc
     4321 attcaagaat acgaagatag gaattgcacg tgatttacca tttgttaaat attgatcgat
     4381 cagttttgta tcatcatctc tatggaacac gcgcacttct aaatttaatg cttcactgat
     4441 gtgttttaga attgggagat tcatcattgc atctccacac cagtcttcag taattactaa
     4501 tactttagaa taattcatct cttttatttt tttgatgcgt gaatcatctt ctggtaactc
     4561 aaatgattga tagatactga gaacggtatc ttgatttgtc ttcattccat caatgtattc
     4621 atttaagggt tggctatttt tgaaataagt ttctaaattt gtcattgtaa aaacctcctt
     4681 tagcatttac aacattatac caatttatag taataaaagg taatgaaata aattaattgc
     4741 aaattctttg ttaatttttg ttaaggatga aacgggaagc accttatgct atatttaaat
     4801 aagtacaaag aaagggtgac atcagtgcgt attcaaaatc gctgggttgt gtttatatta
     4861 tttttaatct gttcttttgg tgtattaatt ggtttatatc aatatcgtca tacgaaaact
     4921 gtggatttgt ctaatcttga aataaacgat attaaattaa atgagaagtt tgataaaaaa
     4981 ggctatgaag taaataaaaa aattaagttt gatcgtttta aattttataa cagtaaagct
     5041 caccctgacc ttaccgttaa agtgagagaa aaggataaca tcgttaaggg gataatatta
     5101 gtaagagatg aaaagataca tactaatttt gatggaggaa ttggttcgcc gataaataac
     5161 gcgattgaaa atcttggatt cggatataaa agaacaaaag ttggcaatga tttctcatcg
     5221 gtaaagtata ttgatagaga taaccattta aaattaaact tactttatca agatttagaa
     5281 attaaacgta ttgaattttt tagtaaatag ctttaggtct taaagttata aaaaacgaat
     5341 gaataatttt attgggatga gtgacctata tgaacaagat tacttattta aatgaattag
     5401 aaacagcact tgatgcgcta cctaggcatt taagagatca aaaaatgtat gaatatgaac
     5461 gttattttta tgagcaagaa cttaatggcg ttgatgaaga ggaaatttta aagaagttaa
     5521 aagacccaca agatgttgca gctgaaacaa aagctagaag tgttattgat tatgctgaat
     5581 ctaaaccaac ttttgaaaat atttcaagag ctgttgctgc ttcattaagt ttaggcattc
     5641 tatctatttt tgtcatcctt ataccagtat ctatagttgg attatttgta ttagcattat
     5701 ttttaatatc acttttgctg ctgttttgtc caattatttt attagcatca gcaatatcca
     5761 gaggaattgt agactcaatt agtaatgtat tttttgccat atcatattca ggattaggat
     5821 tagtatttat cattgtcata tttaagattt tagaatacat ttatcgttta atcttaaaat
     5881 atttactttg gtatattaaa actgtcaaag gaagcgttag aaaatgaaga aattcttttt
     5941 tattgggctt ttagtgtttg ttgtcttttt tacagcagca accattattt ggttcagcta
     6001 tgataaaaac aaatatggta ctaaacaata tgataaaaca ttcaaagacg atgcttttga
     6061 caatgtatct ataaatttgg atagtacaga acttcgtata aagcggggga atcaatttag
     6121 ggttaaatat gatggtgaca atgatatatt aattaatata gtagataaga cgttgaagat
     6181 tagtgataaa aggtctaaga caagaggata tgcaattgat atgaatcctt ttcatgagaa
     6241 taagaaaacg ttaacgattg aaatgcctga taaaatgatt aaacgtttaa atatatcgtc
     6301 tggagcagga agtgttagaa tcagtgatgt tgatttagag aacacaagta ttcaaagcat
     6361 taacggtgaa gtagttatta aaaactcaaa tcttgacgca ctagattcaa agacaaataa
     6421 tagtcctact tatattagta aaagtaacat taaaaatagt aatattaaag ttgttattgg
     6481 tatgctacaa atcgacaaga gtcaaattaa acaatccata tttttaaacg atcatggtga
     6541 cattgaattt aaaaacatgc catcaaaagt agatgcaaaa gcttctacta aacaaggaga
     6601 tgttcgtttt aagtatgata gtaaacctga agatactata ctgaagctaa atccgggaac
     6661 gggtgatagc gtagttaaaa ataaaacgtt tattaatggt aaagttggga aaagcgatag
     6721 tgttttagaa ttttatacga ttgatggtaa tatcaaagtt gaataaataa aggatgtaag
     6781 caccgatatt aggaagcata atttctctaa tatcggtgtt atttatttgt tggcaaaagt
     6841 taagtcggta tctatattgc cagtaaagtg agtgatatta aggtcttgac catctaacca
     6901 tgatttgaaa tctattattt ctggtggcgc attttctccc aatgtaaaat atgcagttaa
     6961 tgtttcaggg tgatacattg atgtatggat ggtgccagac cagcttttga atagtttact
     7021 gtaaatttca tactgaggat tgttgaataa cttaaatgct gtagtcatat ctaaattatc
     7081 attagtttgt gaaatggtac gcgctagtct ttctttagat tcttttgtat aattgcgatt
     7141 ttcatgtgtt aatatttcaa aatgatttgt acatatatta tcataacgaa catctattga
     7201 tctcggcgtc acttcaacaa ttgcatggtt caatgatttg tccatcagta tgtagctaaa
     7261 tgagcttctg tgtggtattt ctttcaataa ttggattgct tctgttacat ttcggcaatt
     7321 ttcaagaatt agacgaccaa tcatataaca tacaaaacca tttgctggtt tcttccggtg
     7381 cataaagtta tagcccatag ttaatcctga ctcattcata ccatccattc ttccagttac
     7441 ccttgataca ggaccaattt gagctaaacc gctatctgta ggttgatata gtaagtagcg
     7501 tccatcataa gttgcagggt ggtaatcgta atttctaacc atgaagtctt tgccttgaaa
     7561 gacagtgcaa ccactttctt ttaaatcggt aaaacgataa tgtccaaagt ttaaaataat
     7621 ttgacgtgtt ggcattttga gtatactttg tagtcccatt aattcttccc atatttgagg
     7681 tgcgtaagtt tggaatattt gataagtttc atttacatct atatcgaaac gtgggacacg
     7741 ttttttccat tctttttctc tattttttag aagaggtgtt tgttgaagcc atttaccagt
     7801 tttaacacct aactcgaaat gtgaacctct aaaagtcata atatctgatg tcacttgttg
     7861 catatcatcg gcccctttct ttttagttgt aatatattgt aaataaatag taatcgtatg
     7921 tatattgaat gtcatgttaa ataaagttat attttactaa atgaaatata aaattgtttg
     7981 aggtgatttc tcggtgtata agacttatca atctgttaaa acatattttt atagatggtg
     8041 gggatattaa gttaaaaact taaaatcatc ttatcataaa taacaatctt aagttagcat
     8101 tcacgataat agtcattgtt aacattagca tataaggtca tgtcacgttg aaacagaggt
     8161 tcctcggcat ttttgaaaat aatcattagc ttgctcgcta tataatttga tgaatatatt
     8221 tcgtaaaagt gggtatttat ttaatgatta ttctatatat gatagtataa tgaaatgtag
     8281 ataggtattt aatttaacag aggtgaaatt gagatgtgga attttattaa atgtgtgttt
     8341 aaattcgtat ttagcttagt tgctattaca acattagttg ctggtgttgg tgtagtagca
     8401 tttgcttata tctttaaaaa agattttgaa gatattgaaa gaaaaactaa agaaattatt
     8461 tctgatatcg aaagtaaaaa taactaataa catttaggca aggacaatat tgattgttct
     8521 tgcctttaat tatgttcaaa gcattaaggt aacaagacaa tatctaagat atcaaagata
     8581 gaaatttttt gacgttgttg ctgattgtaa acataaccat caatttcata attaatagca
     8641 tcaatacgat aaatggttaa gcgtactgaa tctacaaagc cattattata aaatttaact
     8701 tctacaggtt gggcatattg tagcgcctcg tgtagccgaa tgtttagctc agccaattga
     8761 tcatctgata atacaggacg tgtaattttg ttttggtcga taatgtattg ttgaatcgtt
     8821 tcgaattgtt cgggtaatgt tgcaaaagga gcccatttaa tcatgcctct tcccataggt
     8881 atattgttat ctagtaattc tcttggaacg ttacgataat cagtttcttc ttcataactt
     8941 gtcatcctta attcacccca atctgataat tacattatac gaacatgtgt tctattttgc
     9001 aacaaaaatt ttgtggaagc ataaacgcgt taataattaa tgctcgggta agtaaaaaag
     9061 agggattaat taaaatcgaa taatgacaca tcaccagcaa atagttcttt taaagtagtt
     9121 aaacagtttt agctttaagg aaatgataaa tgattgttaa ttctagctaa aatttaataa
     9181 aatgaaaata agactaacat ggaggggtaa aagtaatgac aaatggatat attggttctt
     9241 acactaaaaa gaatggtaaa gggatttatc gttttgaatt aaacgaaaat cagtcacgta
     9301 ttgatttatt agaaacagga tttgaattag aagcgtctac atatttggtg cgtaataacg
     9361 aagttttata tggaatcaac aaagaaggag aacaatgcgg tgttgcgagt ttgaaaattg
     9421 atgacaatgg tgaattgcat ttattaaata aatgtttgtc ttcaaaagct ggtacaggtt
     9481 gttatgtatc gatttcagaa gataaacgat atttatttga agcggtatat ggagctggca
     9541 tcatacgtat gtatgaatta aacacgcaca caggtgaaat tatacgtcta attcaagaac
     9601 ttgcgcatga ttttccaaca ggtacacatg aaagacaaga tcatccacac gcacattata
     9661 ttaatcaaac tccagatggt aagtacgttg cagtaacaga tttaggtgct gatcgtatcg
     9721 ttacttataa atttgatgac aacgggtttg aattttataa agaatcttta tttaaagata
     9781 gtgatgggac aagacatatt gaatttcatg ataatggaaa atttgcttat gtcgtacacg
     9841 aattatccaa tactgtgagt gttgcagaat ataatgacgg taaatttgaa gagctcgagc
     9901 gtcatttaac aattcctgaa aactttgatg gagatactaa acttgcagca gtgcgtttgt
     9961 ctcatgatca acaattctta tatgtatcta atagagggca tgatagcatt gcaattttta
    10021 aagttcttga taatggtcaa cacttagaac tagtaacaat tactgaaagt ggtggtcaat
    10081 tcccaagaga ttttaatatt gcctcatcag atgacctttt agtttgtgct catgagcaag
    10141 gagattcagt tgtaactgtt ttcgaaagaa ataaagaaac aggtaaaatt acgctatgtg
    10201 ataacactcg tgtagcatct gaaggtgtat gtgtcatatt ttaatcttta attaatcatg
    10261 ataaaaagaa aaccatgttt ccaaaaaatt tgtgtatacc ttgaaattta ttgttttcaa
    10321 gaacatcaaa ttatggaagc atggtttatt tttgtctcaa aaattaattt ttaaagaaag
    10381 ctttgatgta tttaaattta cctttatatg gtggtaaatg gacacctgat tctaatcgtg
    10441 tagatttgaa aatgtagctt ttttcatgtg taaaagtgtc aaatgaatat ttaccatgat
    10501 agcgtcccat acctgaggcg ccaacaccac caaatggtaa tttaggattc gctaggtgca
    10561 tcaatgtatc attaatagcg ccgccgccaa atgataactc gtttattaca cgctgcgtag
    10621 cattttcatc ttcgctaaat aaatataaac tcaaaggttt tggtctttgg tgaataaagg
    10681 ctattgcttc atccaatgac tgatacgtta aaatcggtaa gataggacca aaaatttctt
    10741 cttgcataat tgctgaatca tttgtaacgt gatctaacaa tgttggttct atataacgtt
    10801 catcctcatc actatgacca ccaaatacaa tattcatttg tgcactgtta agtaatgacg
    10861 tcagacgatg ataatgttta aggtttacaa tgcggccata atctggactt tgttgtatat
    10921 tttgaccata aaattcacgc aacgtttttg atagggctgt gattaaatca tcttttacag
    10981 attcgtgtac taaaatgtaa tctggtgcaa cacatgtttg gccggcattg gtgaatttcc
    11041 caaaacaaat gcgctcactg gcaactttaa tattcgctgt ttcatcaacg atgactggag
    11101 atttaccgcc catttctaat gtcacaggaa ctaaattttc gctggcagct tgataaacga
    11161 ttttgcctac attttcactt cctgtaaaga agacatagtc aaaaggtaag tgaattaacg
    11221 tttgcgtttc ttcaattcct ccttcaataa cttcaatgta atttgcatca aatgtttcat
    11281 tgattaatcg tttaatcact cgtgcaacat ttggtgtcaa ctcagatggt ttaataattg
    11341 ctgtattacc tgctgcaata gcaccgatta aaggttcgaa cactagttga aaaggatagt
    11401 taaatggtgc aatgatcaaa actgttccat aaggttcttt tttgatatag ctttttgttg
    11461 gaaataaata taaaggtgtg tctacatttt tggttttagt ccagttttta agttccttac
    11521 gggcattttt gatacttttc aaagttatgc caatttcagt agcgtaagct tcgactttat
    11581 tttttcctaa atctgtatat agtgcttcta aaatatcgct ctcgtatgat ttaatagctt
    11641 tgcttaactt ctttaattgc tcttttctaa aactaatatc tttagtttgt tgtgtattga
    11701 aaaaagcttt actgtcataa aatttttgct caatgatatt cataatgaaa agaacctcct
    11761 tatatgatta ttttggaaaa agtgattaat tgatttgaat gttgtggccg ttaattttaa
    11821 atggtctttc gaattatata tgttgaaagt tgaaaataga gcgatgaatc gtgtacataa
    11881 taatatttat aactttaatc ataacgaaaa aggtaggaag aaaacaaaaa tttatactca
    11941 acatcgcaaa tatttcaaga aaatgtaaag gcaaaagggg aattgtatag aaatcactaa
    12001 tctgtggatt aggatagcta aaggaatgaa aactgctatt gaaaaggttg taaattagtc
    12061 aaacgtaaaa aaaaaacagt tcattgaaag tgaaataaat tctactttaa tgaactgtta
    12121 gttaaataca acatgtctat aattagacag taatatagta ttattttgtt aatgcttcag
    12181 tgatttgagg tacgatttgt ttttttcgag aaacaacacc agataagaag gccatgtcat
    12241 cttctaattg aacattgaat gcttcgccaa ctttatcttt ttcagcacct acaactaaaa
    12301 ttttagaatc actattaatg atgtcagtaa caacaagtac aaataagtca tatttttctt
    12361 gtgcacttac agctaacatt tctttttcta aatcttcttt acgatttaac acttcgtcaa
    12421 ggtcaacagc attaacttgt gcaatacgag tcacatagtc acccatagta aatgatttag
    12481 catccatgtt taataagaat tcaactgatt tatcagttgt tgaagcacct gcttttaaca
    12541 tatctaagcc gtacttttga atatcaactt tagcaatatc ttttaattct tcagctgctt
    12601 taacatcttg ttgtgtacat gttggtgatt tgaaaagtaa gctatctgaa ataattgctg
    12661 ataacattaa accagcaatt tcaggtttaa tttcaaagcc acgttctcta aacattttgt
    12721 ataaaattgt agctgtacaa ccaactggtt cagcacgata acataaagga ccagcagttt
    12781 cgaaatttgc aattctgtga tgatcaatta catgcttaat tgtagcagag gcaatcgtat
    12841 ctgaactttg ttggaattcg ttatgatcaa ctaagataac atcttggcca tctaaatcat
    12901 ccgttaataa ttccggagca ggtacattaa atgtatctaa cgcgaattga gtttctgcac
    12961 tcacatcacc taaacggtat gctttggctc ctgaattacc tctaagttgt tcaaattctg
    13021 ccataataat cgcagatgaa attgcatcag tgtctggatt cttatgtccg aaaatatatg
    13081 ttttagccat tgtcaaatat ctcccttgta tattgtattc tttataatta ttttagcacg
    13141 atttaaacgt gttgttctac ctctgcacct aatgagtttt tgaaatgtgc aagtgcccat
    13201 tcatgccctt tttgattaaa tgaagccact ccctcagcag gtactgaaat tttatagcct
    13261 aagttgtatg cagaaattgc tgtatgtaac acgcaaatgt cggtacatac accaacgatt
    13321 tcgacttgat taatacttct ttctctcaat aaactatcaa gcggggtacc aaagaacgaa
    13381 tcatagcgcg ttttatcaat gaaatgtaca ttaggttgcg ctttaattgt ttcgtataat
    13441 ttacctactt taccgtataa ttctctacca cttgtatcta cgatattgtg tggtgggaat
    13501 aatttacttt caggatgatg aatgtcatgt aaataatgta aatccattaa aaagaatata
    13561 tggtcttgat aataattaaa gtcattgata cgagaaacaa taaaatcttc aatattttgt
    13621 ccaggtttac cgcatgttag taagccgtcg tctgcgataa agtcatatga atagtcaaca
    13681 actaatagtg cgcgatgtgt cattcaacat ctctcctata taataataaa ataaactccc
    13741 atctattcat aaatgctaga gtatttttta accctaatgg ttaatatata aatatagatt
    13801 aagtatatag attaatcaac ttttttggaa gagcaaatca cgcaatcaac aaatattata
    13861 agatgttttt acgatagctt taaaatagct gtaatagaat actaaatgtg acaaacttag
    13921 aactaatatc aagtgttgat gttttcaata taaaaatgct aatagatggc atcacgagat
    13981 ttatgactat ctgctaaatc aacctaacac gattataatt tgaataattg gttaatatat
    14041 gaagtaatta gaaaatagac aaaggatgac gatttatgta tatcaatatg aaagattatg
    14101 ggttaacagg cataaacaaa actaaagata ctcgagcaat acaacgtgcg ttaaatcatg
    14161 gaagatgtaa accaacgaca gtttatatac cgaaagggac gtatgatatt tgcaaaccat
    14221 taacgatata tggcaataca acacttttgt tggataatga aactatttta cgtcgatgtc
    14281 attctggtcc gttattaaaa aatggtcatc gctttggttt ttatcgtggt tataatggac
    14341 acagtcatat tcatattaaa ggcggcaagt ttgatatgaa tggcgtatcg tatccttata
    14401 ataatacagc tatgtgcatt ggacatgctg aagatattca attaataggt gtaaccatta
    14461 agaatgtagt gagtggtcat gcaattgatg cttgtgggat taatggactc tatattaaaa
    14521 actgttcatt tgaaggattc gtagactata gtggcgaacg tttttattct gaagcaatac
    14581 aattagacat tcaagtacct ggtgcttttc caaaattcgg aacgacagat ggtacgataa
    14641 cgaaaaatgt cattatcgaa gattgttatt ttggaccttc agaattgccc gaaatgggaa
    14701 gttggaatcg tgctattggc tcacatgcaa gtagacataa tcgttactat gagaatatta
    14761 atattagaaa taatatattt gaagatatac aaggttatgc attaactccc ttgaagtata
    14821 aagatgcttt cattattaat aataagttta ttaactgtga gggtggtatt aggtatttag
    14881 gagttagaga tggtaaaaat gctgcagatg tgatgacagg aaaagactta ggttcccaag
    14941 caggaataaa tatgaatata attggaaatg aatttaaagg gtcaatgtct aaagatgcga
    15001 tacatgtacg taattataat aatgttaaac ataaagatgt attaatcgtt gggaatacat
    15061 tcaataattc gactcaatca attcatttgg aagatattga tacagtgttt ttaagtcctg
    15121 ttgaagcggg tattcaagtt actacaatca atgtagatga aataaaaaag taaaaagttt
    15181 cgcatgacat taggattaag attactggat aatttttgaa agcgcattca taaaacggta
    15241 taaatatgct ataataaacc caattatctg ataaaagggg tattttgacg gtaatgataa
    15301 tacaagatag acaactttct atactctaat atagtgagtt gaagtagctt gtcataatca
    15361 tcatgagggg gaaatttatg gcttatttca atcaacatca atcaatgata tcgaaaaggt
    15421 atttaacatt cttttcaaaa tcaaagaaaa agaaaccgtt tagtacggga caacttattg
    15481 gactaatatt aggtccatta cttttcctat taacattatt gttctttcat ccacaagact
    15541 taccttggaa aggcgtctat gtcttagcga ttactttatg gattgcgact tggtggatta
    15601 ctgaagcaat tcctattgca gcaacgagct tattaccaat tgtgttatta ccattaggtc
    15661 acatacttac accagaacaa gtatcatccg aatatggcaa tgatattatc tttttgtttt
    15721 taggtggatt tattttggca attgcaatgg aaagatggaa tttacatacg agagtagcgt
    15781 tgacaattat taatttaatt ggtgcaagca cttcaaaaat attacttgga ttcatggtgg
    15841 caacaggatt cttatctatg tttgtatcga acactgcagc tgtaatgatc atgattccga
    15901 ttggtttagc aattattaag gaagcacatg atttacaaga agccaatacg aatcaaacaa
    15961 gtattcaaaa gtttgaaaaa tctctagttt tagcaattgg ctatgcaggt acgattggcg
    16021 gattaggtac acttatcgga acgccgccgc taattatttt aaaaggacaa tacatgcaac
    16081 attttggaca tgaaattagt tttgctaaat ggatgattgt agggattcca acggtcattg
    16141 tattgttagg tattacttgg ctctatttaa gatatgttgc gtttagacat gatttgaaat
    16201 atttacctgg tggtcagacg ttaattaaac aaaagttaga cgagcttggc aaaatgaagt
    16261 atgaagaaaa ggtagtacaa actatctttg tacttgccag cttattatgg attacaagag
    16321 agtttcttct gaaaaaatgg gaagttacgt catccgttgc agatggtacg attgctattt
    16381 ttatatcaat attattattt attattccag ctaaaaatac tgaaaaacat cgccgtatca
    16441 ttgactggga agttgcaaaa gagctccctt ggggtgtatt aattttattt ggtggcggtt
    16501 tagcattagc gaaaggtatt tctgaaagtg gtttagcaaa atggttaggc gaacagttga
    16561 aatcattaaa tggtgtcagc ccgattctta ttgtaatcgt cataactatc tttgtcctat
    16621 ttttaactga agtgacatct aataccgcaa ctgcaacgat gattttaccg attttagcaa
    16681 cgttatctgt tgctgttgga gtgcatccat tactacttat ggcacctgca gctatggcgg
    16741 ctaactgtgc atacatgtta ccagtaggga caccaccaaa tgcaattatc tttggttcag
    16801 gtaaaatatc tatcaaacaa atggcatcag taggattctg ggtaaactta atcagtgcaa
    16861 taattattat tttagtcgtg tattatataa tgcctatagt tttaggtatt gatataaatc
    16921 aaccactgcc attgaaatag taattgcaga ttagaacgaa aaataaaatg ttacattagc
    16981 aattgcttga acgagtggta acgaaacgta taccgtagca tcgtgtaaaa acaatacaaa
    17041 caaaagaaag tcaaccaagg atggattcct attttaatcc ttggttgact ctttatttta
    17101 tttaaattgt agaacctaga aaataaagtt taattaaaag caccaatcat ttctactttg
    17161 aaatctaagg tttctaaaat agcaatgact ttctttatat cggttgtaat tgcagaatca
    17221 gcctgaacga aaaatcgata catacctaat tgtgttttta aaggacgaga ctcaatccag
    17281 gataaattaa tattaaacaa agcaaatgta ttaagcacac ttgctaacaa cccaggttta
    17341 tcatgcattg gtgtaattaa aaacatcaat gatgtcgcat tttgatcaaa ttgctgctga
    17401 ttttttataa ctaaaaaacg tgtcacgtta tgtggatagt cttcaatatg tgtatcaata
    17461 ggtgtaaaac cataagcttc accactacct aaaggtgcaa ttgctgcaac gccattttca
    17521 attttagtca aactttgaat tgtactgtcg acataatcat agtcaaattg atgttgatgt
    17581 atgtaatttg ttgtttgact gattgctggt gcaatagaat acactttttt aatatcagaa
    17641 atggtgttcg aaccattacc atataatgca aagttaatat ctaaacgtat ttcaccgtgt
    17701 gcaaagacat cttgctgtgc aagtgcatct gccacaatgt tgattgttcc ttctatagaa
    17761 ttttcaatag ggacaacacc aatcgatgtg tcatcatctg caactgactt gatgacttca
    17821 aataaatttg actttggttg aaaagttgct tcattttcag aaaaatactg acgacaagcc
    17881 aaatatgaaa atgtaccttt agggcctaaa taatataatt gcatatgcta tacctctact
    17941 aacttaatga tggaaagggc actggttagc atttgatttt ttctttttat agaaaaagtt
    18001 tggatctttt actgtattgt catatccgtg atgataattt gacgtcaatg ttggagataa
    18061 tggcggtgct agccaagacc attttccggt aacttgacga ccttgttgtg cttcattacg
    18121 ttcgaatagt tcgaattgct ttgcagcggt caaatgatcg acaattgata cgccttcttt
    18181 tttaaaggaa tgatacacag catagttcaa ttcaacaagt gctcgatctt tattaaatga
    18241 attattttta agtgtatcaa attcaaacgc atctgcaact ttttctagta aattgtaacg
    18301 gtaatcatca ataaagttac gtacgccaat ttcagttacc atataccaac cgttaaaggg
    18361 ggcagttgga tacacaatgc caccgatttt taagtccata ttggaaatga tagggactgc
    18421 ataccatttt aagttcaatt ttcttaattt tggataatga tcatgttcaa taggtacttc
    18481 tttaattaat gacgtaggat attcgtaata tttaactgac tcattaggta attggtaaat
    18541 cagtggtaac acgtcaaaat tagtaccttt tcctttccaa cctaagtgat ttgctaagcg
    18601 tgtaacttct ttttcagcag gatcaccaca attgtcatag ccagcatagc gaattaattg
    18661 attgttgaaa attttaggtc catcctttgg agcatatata gtaatatacg gctttaattt
    18721 accttcatta gtagcctgtg caatatgata attaattgat gataagaacg atgcttcgtc
    18781 agtaacatct cttgcatcaa tgacatttaa cgaatcccaa aataaacgac caatgcaacg
    18841 atttgaatta cgccaagcca ttttagcacc ataaataagt tcttcttctg tatgtgtata
    18901 tgtcccagtt tcttttattt ctagttcaat gtcatgtaaa cgtttattga taatttgcgt
    18961 ttcataatga cactctttat acatgttttc tatgaaagct tgagcctctt taaataacat
    19021 taacaacacc tcgctttata ttatagtcta cattattaaa atactcttaa aaattatgta
    19081 tatgtcatta aattgttggt tgattttaat taaaagtatg gaaattaagg ggctcttatg
    19141 tatataaaaa aatgaattat gataaaatgt aagaaaatat ttaggtcgat tggagagata
    19201 cgagtgtacc aattagaaga cgacagttta atgttacata atgacttata tcaaataaat
    19261 atggctgaaa gttattggaa tgataatatt catgaaaaaa tggctgtatt tgatttatat
    19321 tttagaaaaa tgccatttaa tagtggctat gctgttttta atggtttaaa acgcgtcatt
    19381 gattttatag aacattttgg attttcagaa tctgatttgg aatatttaaa gtctattggc
    19441 tacaaggatg atttcttatc atatttaaaa gatttaaaat tcactggcag catccgttcg
    19501 atgcaagaag gcgaattatg ctttggtaac gaaccattgt tacgcgtaga agcaccattg
    19561 attcaagcgc aattaataga aacaatttta ttaaacattg taaatttcca tacactaatt
    19621 acaacaaagg ctagtagaat tcgtcaaatt gcatcaaatg ataaattaat ggagtttggt
    19681 acacgtcgtg cgcaagaaat tgatgcagca ttgtggggcg ctagagctgc ttacatcggg
    19741 ggctttgatt ctacaagtaa tgttagggcg ggtaaattat ttggtatacc tgtgtctggt
    19801 acacatgcac acgcatttgt ccaaacttat ggagatgaat atgttgcctt caaaaaatat
    19861 gctgaaagac ataaaaattg tgtgttccta gtagatacat tccatacttt aaaatctggc
    19921 gtgccaaatg caataaaagt tgcaaaagaa ttaggtgaca aaattaactt tgtaggtatt
    19981 cgattggatt ctggagatat cgcttattta tctaaagagg ccagacgtat gcttgatgaa
    20041 gcaggattta ctgaaactaa aattatcgcg tctaatgatt tggatgaaga aacgattacg
    20101 agtttgaaag cacaaggtgc aaaagtagat tcttggggcg ttggtacaaa gctgattaca
    20161 ggatacgatc aaccagcatt aggtgcagta tataaacttg tagctattga aaatgaagat
    20221 ggttcatata gtgatcgtat taaattatca aataacgctg aaaaggttac tacgccaggt
    20281 aagaaaaatg tatatcgcat tataaacaag aaaacaggta aggcagaagg cgattatatt
    20341 actttggaaa atgaaaatcc atacgatgaa caacctttaa aattattcca tccagtgtat
    20401 acttataaaa tgaaatttat aaaatctttc gaagccattg atttgcatca taatatttat
    20461 gaaaatggta aattagtgta tcaaattcca acagaagatg aatcacgtga ttatttagca
    20521 caaggattac aatccatttg ggatgaaaac aagcgtttct taaatccaca agagtaccca
    20581 gtcgatttaa gtaaagcatg ttgggataat aaacataaac gtattttcga agtggcggaa
    20641 catgttaagg agatggaaga agataatgag taaattacaa gacgttattg tacaagaaat
    20701 gaaagtgaaa aagcgtatcg acagtgcgga agaaattgct gagttaaaac aatttataaa
    20761 aagttatgtc caatcacatt catttataaa atctttagtg ttaggtattt caggaggaca
    20821 agattctaca ttagttggaa aattagtgca aatgtctgtt aacgaattac gtgaagaagg
    20881 cattgattgt acgtttattg cagttaaatt accttatgga gttcaaaaag atgctgatga
    20941 agttgagcaa gctttacaat tcattgagcc agatgaaata gtaacagtca atattaagcc
    21001 tgcagttgat caaagtgtgc aatcattgaa agaagctggg attgtcctaa cagacttcca
    21061 aaaaggtaat gaaaaggctc gcgaacgtat gaaagtacaa ttttcaattg cttcaaaccg
    21121 acaaggtatt gtagtaggaa cagatcattc agctgaaaat ataactggtt tttatacgaa
    21181 atatggtgat ggtgctgcag atattgcacc tatattcggt ttgaataagc gccaaggtcg
    21241 tcagttatta gcttatcttg gtgcaccaaa acaattgtat gaaaaaacac caactgctga
    21301 tttagaagat gacaaaccac agcttccaga tgaagatgca ttaggtgtaa cttatgaggc
    21361 gattgataat tatttagaag gtaagccagt tacgccagaa gaacaaaaag taattgaaaa
    21421 tcattatata cggaatgcac acaaacgtga acttgcatat acaagataca cgtggccaaa
    21481 atcctaattt aattttttct tctaacgtgt gccttaaatt aaatatgagt tagaattaat
    21541 aacgttaaac cacattcaac taggctactt cagtatataa attgataatg tatgaaataa
    21601 agtaagtatg ttcatttgag aataaatttt atttatgaca aattcgctat ttatttatga
    21661 gagttttcgt actatattat attaatatgc atttattaag gttagtttga agcagtttga
    21721 tatttaaagt gtaattgaaa gagagtgggg cgccttatgt cattcgtaac agaaaatcca
    21781 tggttaatgg tactaactat atttatcatt aacgtttgtt atgtaacgtt tttaacgatg
    21841 cgaacaattt taacgttgaa aggttatcgt tatattgctg catcagttag ttttttagaa
    21901 gtattagttt atatcgttgg tttaggtttg gttatgtcta atttagacca tattcaaaat
    21961 attattgcct acgcatttgg tttttcaata ggtatcattg ttggtatgaa aatagaagaa
    22021 aaactggcat taggttatac agttgtaaat gtaacttcag cagaatatga gttagattta
    22081 ccgaatgaac ttcgaaattt aggatatggc gttacgcact atgctgcatt tggtagagat
    22141 ggtagtcgta tggtgatgca aattttaaca ccaagaaaat atgaacgtaa attgatggat
    22201 acgataaaaa atttagatcc gaaagcattt atcattgcgt atgaacctcg aaacatacat
    22261 ggtggattct ggactaaagg cattcgtcgt agaaagctta aagactatga accagaagaa
    22321 ctggaaagtg tagtagaaca tgaaattcaa agtaaatgag aacgaaacaa ttgcagattg
    22381 tttgtcacga atgaaaatgc aagggtatat gccggtaaaa cgtattgaaa aacccgtgtt
    22441 tcaggagcaa aaagatggca cggttgaagt atcacatcaa gaaatcattt ttgtaggtaa
    22501 gaaaatccaa taacataatc caatttaaat aaagactatt tgaggaggaa aggctattca
    22561 aagtttgagt aattttactt tgaatagcct atgtgtttat acatgcaaga tgctcgatcc
    22621 atattgtatg agaaaccccc agcaagctat ataaagtata tgctgggggt tcttaatatt
    22681 ttaaaatcat tgttaaatta tatatatcgt cgctttttct aaaaaaatct cattgcatga
    22741 aattttttct tcctagagtc ctttaataag agtaatagtt tacttaaata tatctagata
    22801 gtcttattat ttatgcttaa tgaaagtcat tctaggagaa gttcctaaag cttctgtgtt
    22861 cataattgtt agtagtattt tattatcatt tggtataaat atttcaataa caattgagct
    22921 attattttta ttatataatg tgagttgttt gtgttctgta tttatatatt tgcaaatgt
//
LOCUS       DAKSQE010000031        22871 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.031, whole genome shotgun sequence.
ACCESSION   DAKSQE010000031 DAKSQE010000000
VERSION     DAKSQE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22871
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.031"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            221..1219
                     /locus_tag="O0V33_001078"
     CDS             221..1219
                     /locus_tag="O0V33_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCX8975725.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNNMMIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
     gene            1451..2332
                     /gene="rbsU"
                     /locus_tag="O0V33_001079"
     CDS             1451..2332
                     /gene="rbsU"
                     /locus_tag="O0V33_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HCX8975726.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGFPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            2347..2751
                     /gene="rbsD"
                     /locus_tag="O0V33_001080"
     CDS             2347..2751
                     /gene="rbsD"
                     /locus_tag="O0V33_001080"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCX8975727.1"
                     /translation="MKKSAVLNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            2779..3693
                     /gene="rbsK"
                     /locus_tag="O0V33_001081"
     CDS             2779..3693
                     /gene="rbsK"
                     /locus_tag="O0V33_001081"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCX8975728.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKAAHIDTSYIIKTAEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSLGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(3791..3925)
                     /locus_tag="O0V33_001082"
     CDS             complement(3791..3925)
                     /locus_tag="O0V33_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975729.1"
                     /translation="MIIIIINIAYLYAIIWKLTRLQKIVTSFITKHESSSIGLVENYF
                     "
     gene            3944..4705
                     /locus_tag="O0V33_001083"
     CDS             3944..4705
                     /locus_tag="O0V33_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCX8975730.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYHQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIINN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKEH"
     gene            complement(5200..6636)
                     /locus_tag="O0V33_001084"
     CDS             complement(5200..6636)
                     /locus_tag="O0V33_001084"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCX8975731.1"
                     /translation="MTKLPQNFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKA
                     RQITESIDPNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVNYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDFGWQEGDDSILQQGTVDYIGFSYYMSTSVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGRGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(6652..7443)
                     /locus_tag="O0V33_001085"
     CDS             complement(6652..7443)
                     /locus_tag="O0V33_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCX8975732.1"
                     /translation="MGNKYKDYAQDILTAVGGVENIVNVSYDTVRMTIHMHHAIPSTA
                     NEVTQIDGVASVDENETQLVIVFNEEVKYVYQQLQLLMDDAKHQEDTNHDAVDTQETE
                     QKAQAKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     VSMIVPNKHAVGIQSEDGVDIVIHIGVNTVDLEGKGFECFVKQNDRVEAGQTLLQFDQ
                     QYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            7590..8294
                     /locus_tag="O0V33_001086"
     CDS             7590..8294
                     /locus_tag="O0V33_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCX8975733.1"
                     /translation="MLKYEHIAKQLNAFIHQSNFKPGDKLPSVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEI
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDQLTSSEASLLQLSAGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(8520..10811)
                     /locus_tag="O0V33_001087"
     CDS             complement(8520..10811)
                     /locus_tag="O0V33_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HCX8975734.1"
                     /translation="MSMQSSYKRLVKVLLTILVIVFSILLVGGWYIFKNEAPRPTKIV
                     DQQGHTLVTKGELISGQAIYEKYGLTDYGSYLGNGSYLGPDYTAEALHHYLIGMRKYY
                     AQDIYHKQLHKLNHAELAVVKDKVMKEIRINRYSEKEDQLVLTPGQVYGLKYLQDYYK
                     KEFVNNPKQVGLNENMIKQFQNDDYMVAGNKVEHLSQFFFWGAWLSSTDRPGKTFSYT
                     NNWPYDVDAGNTLPSAGILWTAISVTLLIAGLAFIIYIQKRYEFDMKPTYESERELPK
                     IEVDSKITDSQRKVGKYLVVVMLLFLVQILLGELLAHYYVENKFFGIEIQRLFPFNIA
                     KTWHVQLVIFWVATTWLAAGIYIVPKVLGKEPKKQGVLVDILFWALIIVVMGSMIGEW
                     GHILGWIDSHWWLFGHFGWEYIELGKFWQILFIVGMVLWVIILCRGFLPAIRNKVQAN
                     HDKRHLLTLLFVGAIAIPLFYLASLFIMPNSHVTFADYWRWWIVHLWVEGIFEAFAVV
                     LIGFLMVNMKLTTIETTIRALYFQLILLLGTGIVGMGHHYYWQGDHSIWLALGSCFSA
                     LEVVPLCLLIWEAYTHYRLYKKSDIAFPYKGTFIFLASTGLWNAIGAGALGFLINTPA
                     VNYFEHGTQWTAAHAHGSMAGVYGMFSIAIALYVFKNITKKEFWTPKVEKWIKISCWA
                     LNVGLAGMVFITLMPVGYIQLKDALEHGYWHSRLTSFYEQPLVKAIMWGRMPWDIIFT
                     VGVIILVVIFIRGYRHLKPKYRV"
     gene            complement(11152..11853)
                     /gene="lrgB"
                     /locus_tag="O0V33_001088"
     CDS             complement(11152..11853)
                     /gene="lrgB"
                     /locus_tag="O0V33_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HCX8975735.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(11846..12289)
                     /gene="lrgA"
                     /locus_tag="O0V33_001089"
     CDS             complement(11846..12289)
                     /gene="lrgA"
                     /locus_tag="O0V33_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HCX8975736.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(12402..13142)
                     /locus_tag="O0V33_001090"
     CDS             complement(12402..13142)
                     /locus_tag="O0V33_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HCX8975737.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNSANAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPAYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(13145..14899)
                     /locus_tag="O0V33_001091"
     CDS             complement(13145..14899)
                     /locus_tag="O0V33_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCX8975738.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRNVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
     gene            complement(15150..15824)
                     /gene="scdA"
                     /locus_tag="O0V33_001092"
     CDS             complement(15150..15824)
                     /gene="scdA"
                     /locus_tag="O0V33_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HCX8975739.1"
                     /translation="MINKNDIVADVVTDYPKAADIFRSVGIDFCCGGQVSIEAASLEK
                     KNIDLNELLQRLNEVEQTNTPGSLNPKFLNVSSLIQYIQSAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKNGMLEHMQKEDDVDFPKLIKYEQGEVVDDINTVI
                     DDLVSDHIATGQLLVKMSELTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
     gene            complement(15964..17688)
                     /gene="tarS"
                     /locus_tag="O0V33_001093"
     CDS             complement(15964..17688)
                     /gene="tarS"
                     /locus_tag="O0V33_001093"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCX8975740.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     ALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKDQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFEGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISMKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMVMGMLSQTSENEVQILLSKDIKKRDFKNIVKLNTA
                     HITYPLNK"
     gene            complement(17721..19409)
                     /locus_tag="O0V33_001094"
     CDS             complement(17721..19409)
                     /locus_tag="O0V33_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCX8975741.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            complement(19431..20456)
                     /locus_tag="O0V33_001095"
     CDS             complement(19431..20456)
                     /locus_tag="O0V33_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCX8975742.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEFEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            complement(20449..21165)
                     /locus_tag="O0V33_001096"
     CDS             complement(20449..21165)
                     /locus_tag="O0V33_001096"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HCX8975743.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
     gene            complement(21441..22610)
                     /locus_tag="O0V33_001097"
     CDS             complement(21441..22610)
                     /locus_tag="O0V33_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCX8975744.1"
                     /translation="MIKTTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQHAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVQNQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGHASQRICQTIFK"
BASE COUNT         7377 a         4287 c         3415 g         7792 t
ORIGIN      
        1 acattttaaa attctagtta tcataaccat taaaaggtat taaccttaat aatatattct
       61 ctcgcctcaa ccttaatcgc atactatagg cgtctgtttg tagacaataa aagtcattca
      121 cgtcttcata tgtcatcaaa tgtttatcat gatatgatga atataataat cgggtatata
      181 actgtatgat taattacaca ataaagagaa ggaatataat atgaaaaaag tgtcaattaa
      241 agatgttgct agagaagctg gtgtatcagt cacaactgtg tcacatattt taaatcataa
      301 tgatagtcgt ttttccgcaa caacgataaa aaacgtacat gctgtttcag aacgtttagg
      361 ctacgcccct aacaaacatg caaaacaatt gcgcggcagt aaaattcaaa ctattggcgt
      421 cattttacct agcttaacaa atccgttttt ctcagcactg atgcaaagta ttcatgacca
      481 taaaccatct gatgttgatt tatgcttttt aacatctaca gcaactgatt tgtatgacaa
      541 tattaaacat ttaattgatc gaggtattga cggattaatt atcgcacaat acatttcatc
      601 cccggacgcc ctaaataact atctaaagaa acatcatgta ccttatgtcg tactggatca
      661 aaatgaccat caaggctata cagattttgt tcggacaaat gaatatcaag gcggacaact
      721 tgcagcacaa catttaatag aactcggtca caacaatatg atgattgttg caccatatga
      781 catgatggcg aatatgtcga ctcgtgtcgc tggatttgtt gatactttgc gcgcgaatca
      841 attgccagaa ccacaaatcg tccatactga attatctaag cgtggtgggc taaccattgt
      901 tgatgacatc atggttcaat ctgccactgc aatcttcgct attaacgatg aactcgctat
      961 tggcatttta cgaggactaa ttgaacatgg catcagtatc ccgaaagata tctcattaat
     1021 aggttatgac gacattgatt atgcagcgta cgtctcgcca cctttaacta ctgtggcaca
     1081 acctataact gatattggca aaacatcttt aaccttatta cttcaacgat tacagcactt
     1141 agataaatcc attgatatga ttgaattacc aacgacttta aaaattcgtg caacaactgg
     1201 ctatcatctt tcaaactaac tacgtatctt ccgaaatata ctcatcattg ttaggccctt
     1261 agcgttgctt taatgctgag ggtttttaat cataattatt tcactatgaa attaaaataa
     1321 taatgtatga attttcaaat atgatttaaa cgttttcagc ttttatgaaa acgcatgcat
     1381 tttacaaata aaaatggtac gatggcactg gtaaaacgtt ttactaaaaa caaatcgtga
     1441 ggtgtataac atgagcattg ttgcattact tatcgggtta ggccccttaa ttggctgggg
     1501 cttcttccca acagtcgctt caaagtttgg tggtaaacct gtacatcaaa ttatcggtgc
     1561 tactgtaggt acgttaatct tcgctattat attagccgta gtcacatcaa gtggctttcc
     1621 tactggaacc aatttgctat tcgccttatt atcaggtgca ggatggggat tcggacaaat
     1681 cattacattt aaagcgttcg aattagtcgg ctcatctcgt gccatgccag tcacaacagc
     1741 attccaatta ttaggcgcat ctttatgggg tgtctttgca ttaggaaatt ggccaggcat
     1801 tggtcataaa atcattggat ttacagcttt agtcgttatt ctaattggtg cgcgtatgac
     1861 agtttggagt gaacgcaaag aagcaagtaa cgccaaaaat ttacgtcgtg cagtggtact
     1921 tctgttaatt ggtgaatttg gatactggtt atattcagct gcaccgcaag caacttctat
     1981 tgatggccta actgcctttt tacctcaagc aatgggtatg gtaattgttg cagtcattta
     2041 tggctttatg aatatgaaag cagagaatcc attccgtaat aaaattacgt ggttacaaat
     2101 tatttcaggt ttcttctttg catttggtgc cttaacatat cttatttcag cacaacctaa
     2161 tatgaatggt ttagcaactg gatttattct ttctcaaaca tccgttgtgc ttgctacatt
     2221 aactggtatt tatttcttaa aacaacataa aacgtcaaaa gaaatggtta ttacaatcat
     2281 cggcttagta ctcattttag tagccgcttc tgttacagta tttataaaat aaggagtgta
     2341 gatgtcatga aaaaatcagc tgttttaaat gaacatattt caaaagcaat cgcgacaatt
     2401 ggtcattttg atctattaac aattaatgac gctggcatgc caattccaaa tgatcatcgt
     2461 cgtatcgacc tagctgtcac aaaaaactta ccgcgcttta ttgatgtctt agctacagtg
     2521 ttagaagaaa tggaaatcca aaaaatatac ttagcagaag aaataaaaga acataaccct
     2581 acacaattgc aacaaattaa acaattgatt tcatcggaaa tcgaaatcat tttcattcct
     2641 cacgaagaaa tgaaaagtaa cttagctcac ccattaaata aaggtaatat tcgtactggt
     2701 gagacaacgc cctactctaa tattgcatta gaatcgaatg ttacttttta aaaattataa
     2761 cttgaaagga gcatacacat gaccaacaaa gttgttattt taggttcaac gaatgtcgat
     2821 caatttttaa cagttgaaag atatgcacaa ccaggcgaaa cattacatgt tgaagaagca
     2881 caaaaagcat tcggcggagg taaaggtgcc aaccaggcta ttgccactgc acgcatgcaa
     2941 gcagacacaa catttattac taaaattggc actgatggcg ttgctgattt catcttagaa
     3001 gattttaaag cggctcatat tgatacgtca tatattatca aaacagctga agcaaaaacg
     3061 ggccaagcct ttatcactgt gaatgcagaa ggacaaaaca ccatctatgt ttatggtggt
     3121 gcgaatatga cgatgacacc tgaagatgtt attaacgcaa aagacgctat aatcaatgca
     3181 gactttgtcg ttgcacaatt agaagtaccc atcccggcta ttatatctgc atttgaaatt
     3241 gccaaggcac atggtgtgac gactgtatta aatcctgcac cagcgaaagc attacctaat
     3301 gaattattgt cattaatcga tattattgtg ccaaacgaaa cagaagccga attgttatct
     3361 gggattaaag taacaaatga acaatctatg aaagacaatg ccaattactt tttatcttta
     3421 ggcattaaga ctgttttgat tacgctaggt aagcaaggta catattttgc tactaaaaat
     3481 caaagccaac acatcgaagc ttataaagta aatgcgattg atacaactgc tgcaggcgac
     3541 acatttattg gtgcatttgt cagtcgctta aacaagtcgc aagataactt agcagatgct
     3601 attgattttg gtaataaagc gagctcactc actgtacaaa aacacggcgc gcaagcatct
     3661 attcctctac tagaagaagt aaatcaagtt taaatgaatc aaacacagct atgatatgaa
     3721 ggtttagcat ataacatgca acattcgtat atcatggctg tgctttttta tctttataaa
     3781 acatcatcta ttagaaataa ttttccacta aacctatgct tgaagactca tgtttagtta
     3841 taaatgaagt gacaattttt tgtaatcttg ttaacttcca aattatcgca tataaatatg
     3901 ctatattaat gataataatt atcaattaaa aggaggttat gttatgtcca aagaagctgg
     3961 tcatacattt ttagctaaat taggaaaaac tcgtctacgc cccggtggta aagaagcaac
     4021 agattggtta atacaacaag gggcattttc acaagataag caagtgttag aagtggcatg
     4081 taatatgtgc acaacatcta tttatctagc tcatacatac ggctgtcaca ttcaaggcgt
     4141 tgatataaat aagaaagcat tagaaaaagc acaggaaaat atttcagcag caggtcttga
     4201 atcatatatt caagttcaac aagcgaatgc tgttaaattg ccctttgatg acaatcaatt
     4261 cgatatcgtt ttaaatgaag caatgttaac aatgttaccc atcgccataa aggaaaaagc
     4321 attacgcgag tactaccgag tcttaaagcc tgggggtatc ttgttaacac atgatattgt
     4381 catcgttaat gaatcacatg ccacacatgt agttaaatca ttatctgcag caattaatgt
     4441 caatgtctca ccgcagacga aacttggctg gttagattta tatcatcaag ctggttttaa
     4501 tcatgtacat tatcatactg gtccaatgag tttaatgaca ccaaaaggtt taatttatga
     4561 cgaaggtatt gttggaacta taaagattat caacaatgct ttaaaaaaag aaaatcgacc
     4621 aatgttttgt aaaatgttta aaacgatgac taaattgcgt aaagatatga attatattac
     4681 ttttgtcgct aaaaaagagc actaaatata atgccactaa ctgtacttta tatctatgtt
     4741 tgactgttac tttaatttct ttgtgacact aatcatctac ttaacaatat cgttatcgtt
     4801 gattagtagg tcatcaattt tggttaaaga cttttataaa tactcaaaca ttaacactat
     4861 acatacttag ttagtggcat tatttttttc ttaaaatttt aacatcacgg gattaggaca
     4921 aaaatgatat tttcgcaaaa tttatttctg tccttcccca acttgcattg cctgtagaat
     4981 ttcttttcga aattctctat gttggggccc cgccaacttg cattgcctgt tgaaattggt
     5041 gttccaattt ctctgtgttg ggggcctgcc aactcgcaca ttattgtaag ctgtatttcc
     5101 gccagcttct atgttggggg cccgccaata taacacttta gggcctacca tattaattta
     5161 tgtctcgggc tctaacccta aaatacttat aatgtacctt tataatttat ctccatttga
     5221 tgctatcact tgttgatacc aatagaacga atcttttttc aagcgttcca tcgtgccacg
     5281 accatcatta tctcgatcaa catatattaa accatagcgt ttcttcattt caccagttgt
     5341 aaatgaaaca atatcaatga ttccccacgg tgtataaccg attaaatcta caccatcttg
     5401 atcaactgct tctatcgctg ctgtaatatg tgcttttaaa tattcaatgc gataatcatc
     5461 atgaatatgt ccatctacca cttcatcaac tgcaccaaaa ccattttcca caataaaaag
     5521 tggcaactga taacgatcat ataacacatt caatgtatat cttaagccat ctggatcaat
     5581 cgcccaaccc caatcactcg ttgcgatatg cggattctcc acagaatgat tcaaaccacc
     5641 gttgacgatg ttgttttcaa ctgtagtatc aacatcatgt tttacagacg tagacatgta
     5701 ataactaaag ccaatataat caaccgtgcc ctgctgtaaa atactgtcgt ccccttcttg
     5761 ccatccaaaa tcatatcctt ttcgtgccaa cattttttta gcatagcttg gataataacc
     5821 tcttacttga acatccggga aaaagaaacg taagcgattc gcaatttgtg cttccatcat
     5881 atctttcgga tgacacgaat atggataaat gggtacatgt gaaatcattg ttccaatctt
     5941 aaatttcgga ttaatctctt taccaagacg aaccgctaaa gcactggcta aaagttcatg
     6001 atgtgctact tgatacaaga cttcttcagg attatcattt tctgtcaatg ctaccccaga
     6061 attcgtccat aaaaagatag gatttgatgt gtccatctga ttatttattt cattaaacgt
     6121 catccaatat gtcactttat ctttatatct ttcaaataca acacgcgcaa aatgcacaaa
     6181 ataattgaca acttctctat ttctaaatcc accataatgt ttcgctaaat gaagtggcat
     6241 ctcaaaatgt gataacgtca caacaggttc aataccttgt gcaattaatt catcaaagat
     6301 acgatcataa aaggcaagtc cttcttcatt tggcacatct tcatccccat tcggaaagat
     6361 acgtgtccaa gcaatcgacg tacgtaaaca tttcaatccc atttctttaa acaaggcaat
     6421 atcttcctta taacgatgat aaaaatcaat accttcatga tttggataat agtgattggg
     6481 atctatagat tctgtaatct gacgtgcttt accatgtgca ccactcgtca taacatcaat
     6541 tacacttaac cctttaccac ctttatcata tccaccttca aattgattgg cggcaagagc
     6601 gccaccccac ataaaatttt gcggtaattt tgtcattatt acaacacact cctagttttt
     6661 aaatatttta aaaataacat cttcagtcgt aatgatttca ttcattgtca gttctacttt
     6721 tcctaaatcg gcagaattgc taataacgac aataacgtca gcattgtagc cttgttgttg
     6781 tatatattgc tggtcgaatt gtaacaacgt ttgccctgct tcaacacgat cattttgctt
     6841 tacaaagcac tcgaatcctt taccttccaa gtcaactgta ttcacaccaa tatgaatgac
     6901 tatgtccaca ccgtcttctg attgaatacc aactgcatgc ttatttggta caatcataga
     6961 tactaaacca ttgaacggtg cgattacttt ggattcttca tgagctttga ttgccaagcc
     7021 ttcgcctacc attttctctc taaaaattga atctcttact tctttaagta aaatacgacg
     7081 accagcgatt ggtgctttca ctaaaattgg tgttgtcact tttgcttgcg ctttctgttc
     7141 tgtttcttga gtatccacag catcatgatt cgtatcttct tgatgcttgg cgtcgtccat
     7201 taacaattgt aattgttgat atacatattt tacttcttca ttaaatacga taactaactg
     7261 tgtttcattt tcatcaactg acgccacgcc atctatttgt gttacttcat tagcagtaga
     7321 aggaattgca tgatgcatat gaatggtcat gcgtaccgta tcatacgata cattaacaat
     7381 attttcgaca ccccccacag ctgtaaggat gtcttgagca taatctttat atttattgcc
     7441 cataagtatt accccctcaa cttgataata gaatggaaat atatgactta tcataattat
     7501 aattgtaccg gttcaatttg taaacgccga tacaattata atattttgtg ctataataat
     7561 tacagacaaa gtgaaacgag gacagaatat tgttaaagta tgaacatatt gctaagcaac
     7621 ttaatgcgtt tatacatcaa tctaatttca aacccggtga taaattgcca agcgtgacgc
     7681 aattaaaaga acgttatcaa gtaagtaaga gtactatcat taaagcatta ggcttattgg
     7741 aacaagatgg tttgatctat caagcacaag gcagtggtat ttatgtgaga aatattgcag
     7801 atgcaaatcg tatcaatgtt tttaagacca atggtttttc taaaagttta ggcgaacatc
     7861 gaatgacaag taaggtactt gtttttaagg agattgcaac gccacctaaa tctgtacaag
     7921 atgagctcca attaaatgca gatgataccg tctactattt agagcgatta agattcgtgg
     7981 acgatgatgt tttatgtatc gaatattctt attatcataa agaaatcgtg aaatatttaa
     8041 atgatgatat tgctaagggc tctatcttcg attatttaga atcaaacatg aaacttcgta
     8101 ttggtttttc agatattttc tttaatgtag atcaactcac ttcaagtgaa gcttcattac
     8161 tacaattatc tgcaggtgaa ccatgtttac gttatcacca aacgttttat acaatgactg
     8221 gcaaaccttt tgattcatct gacatcgtat ttcattatcg tcatgcacag ttttatattc
     8281 ctagtaaaaa gtaatacaaa catcaaaacg tctatattcc agttataaac cggagtatag
     8341 acgttttata ggacataatt tctttaaaaa cagtgagaag tacattttca atcagaaaat
     8401 tactttttac tgttctttat atatacataa tctaagaact tatagtttga atcctattgt
     8461 ataatatctc ttcgtctcaa acgttaaaac acactaagat cactcagtaa taagatgatc
     8521 tatactctgt attttggttt taagtgtctg tatcccctta taaaaataac tactaatata
     8581 atgacaccta ctgtgaatat aatgtcccaa ggcattctac cccacatgat tgcttttaca
     8641 agtggttgtt cataaaatga cgttagtctt gaatgccaat aaccatgttc taatgcatct
     8701 ttaagttgga tataacctac tggcattaat gtaatgaaca ccatacctgc caagccaaca
     8761 tttaaagccc aacatgatat tttaatccat ttttcaactt tcggtgtcca aaattctttt
     8821 ttagtaatat ttttaaaaac atacaacgca attgcaattg agaacatacc atacacgcca
     8881 gccatagaac catgggcatg tgctgcagtc cattgcgtac catgttcaaa gtagttaact
     8941 gctggtgtgt taattaaaaa gcctaatgct ccagcaccaa ttgcattcca caatcctgtt
     9001 gaagcaagaa aaataaatgt acctttgtaa ggaaaagcga tgtcactttt cttatataag
     9061 cgatagtgtg tgtatgcttc ccaaattaat aaacaaagtg gcacgacttc taatgctgaa
     9121 aaacaagaac ccagtgctag ccatatggaa tgatcacctt gccaatagta atgatgaccc
     9181 ataccaacaa tacctgtacc tagtaatagg attaattgaa aatataatgc acgaatcgtc
     9241 gtttcaatgg tagttaattt catattaacc attaagaaac caatcaacac tacggcaaat
     9301 gcttcaaata taccttcaac ccataagtga acaatccacc atcgccagta atctgcaaac
     9361 gtcacatgtg aattcggcat tataaataat gatgcaagat aaaataacgg aattgcaatt
     9421 gctccaacaa acaatagcgt caataagtga cgtttatcat gattcgcttg tactttgttg
     9481 cgaatagctg gtaaaaaccc tcgacaaaga atgataaccc ataagaccat acctactata
     9541 aataatattt gccaaaattt acctaattct atatattccc aaccaaagtg cccgaatagc
     9601 caccaatgag aatcaatcca acctaaaata tgaccccatt ctccaatcat gctgcccatc
     9661 acaactatga ttaaggccca aaacaagata tctactaata cgccttgttt ctttggttct
     9721 ttgcccaata ctttaggtac aatgtatatt cctgctgcta accaagtcgt agcaacccag
     9781 aaaataacta attgcacatg ccatgtcttc gcaatattaa atggaaagag cctttgtatt
     9841 tctataccaa aaaatttatt ctctacataa taatgtgcca gtaattcgcc taataatatt
     9901 tgaaccaaaa atagtaacat gacaacgact aaatatttcc caacttttcg ctgactatca
     9961 gtgattttac tatctacttc aatctttggt agttcccgtt ccgactcata cgtcggcttc
    10021 atgtcaaatt cataacgctt ttgaatatat ataatgaatg ccaagcctgc aatcagtaat
    10081 gttacagata tagcagtcca taaaattcct gcactaggca acgtatttcc tgcatccaca
    10141 tcgtatggcc aattatttgt atatgaaaat gtttttccag gtctatctgt tgatgatagc
    10201 caagcacccc agaaaaagaa ttgacttaaa tgttcaactt tatttcctgc gaccatataa
    10261 tcatcatttt gaaactgttt aatcatattt tcatttaacc ctacttgttt agggttatta
    10321 acaaactctt ttttataata atcttgcaaa tattttagac cgtatacttg tcctggcgtt
    10381 aatacaagtt gatcttcttt ttcagaataa cgattaatcc gtatttcttt catcacttta
    10441 tctttaacta cggctaactc tgcatgattt aatttatgaa gttgcttatg ataaatatct
    10501 tgtgcataat atttacgcat accaatcagg taatgatgta atgcctctgc tgtataatct
    10561 gggcctaagt aggaaccatt gcccaggtag gagccataat ctgttaaccc atatttctca
    10621 tatatcgctt gcccactgat tagctcgcct tttgtaacaa gcgtatgacc ttgttgatct
    10681 actatctttg ttggacgagg tgcttcattt ttaaaaatat accaaccacc tactagtaaa
    10741 attgaaaaca caatcactaa aatcgttagt agcactttaa ctaaacgttt atatgaactt
    10801 tgcatactca tttataaaat ctcctcttaa ttgcattctt acatataagc atacgccgtt
    10861 tttttataaa gtgttgatga cattaagtaa tttcaagaat ttgtcgaaaa atatttaatt
    10921 gaaatttcac aaatgatata acttacattt taattcttag ttaaattaat tcgttctatc
    10981 atttttcaaa agccgcaata cataaaacga acgcctaaat accataaaac ctagtaaaaa
    11041 gtaataaata cataaaaacg tctatatccc agttataaac cggagtatag acgttttttt
    11101 acgataataa caatggctca aattgctatt atcttgctta ggtttttcgt tttagaagaa
    11161 tattgctaca aagacaggca caactgctac aacaactaca ccaactaaca ctaaagctat
    11221 acttgccatt gattcttcta caggtcctaa ttctttggct ggtgctacac ctaatgtgtg
    11281 accacttgtt ccaagtgcta atcctcgggc aatagggtta gtaattcgga aaagcttcaa
    11341 gaatttatta cctagggcat aaataatgac accatttaaa ataactgcta atgatgttaa
    11401 ttcttttata ccaccgatac cagctgatac tggtaacgca atcgctgtag ttgctgcttg
    11461 aggtaacatt gataaaataa catcattggc aaattgtgct aacttcgcaa aagttaaaat
    11521 aattaataac gctacaactg taccgatacc aatacctccg atgatacgat gccaatgttt
    11581 aacaagcact tcacgctttt tatataacgg aatcgcaaaa cagattgttg ctggttctaa
    11641 gaagaagtaa ataatgtctc cacctatttt gtaagtttta tacggaattc ctgttaaata
    11701 gaggaaggcc acaccaaata ccatactgac aaatagcggt gcgaataaga agaaacgatt
    11761 tgttttttca aataatatgg ttgctaagaa aaatggtata acggataaca gtattccgaa
    11821 gtaaggtgtg tttagtgcta agtggttaat catgagcttg tgcctcctct attttgatct
    11881 tttttgtgac tttgtcacct ttagatctcg aagtaacttt cataataatt tgtgtgacat
    11941 agccagtaca aataagtaat agtattgttg agacgattat tagtccaatg attaaaaatg
    12001 gtgcttggct aatgacacct aaagagttaa caactgagat accggctggt acgaagagta
    12061 agccaatgtt atttgttagt gtcgttccta ctttttcgac ttcgcctaac ttaacagcac
    12121 cagtacataa taatacaaat aataatacta aaccgattac tgatgcaggc ataggaattg
    12181 gcataaatga ttcaattatt ttcgatacaa agagtactaa agcaattaca atgacttggt
    12241 gaaaaaagtg tgctggtttt gatgcgtctt tttgttgttt cacgaccatt gcctcctacg
    12301 tttgatttaa ctaaagtata gatggctcac tttgatttgc gtgaatttta gtccgaaata
    12361 caaaatatca taggtaaaat gcataaaaaa aaggattact gttaaagtaa tcctatcgac
    12421 gctttaaaat ctttcataaa tgaacgtcca acttgcatct tgacaccatt tgtcaatatt
    12481 accatataag tgtagttaaa ccattgttgc acttctttaa tgtgtttcgt gttaataata
    12541 tatgaacgat gaatacgtat gaaataagcg ggattcaatc gtttttcata acgattcaat
    12601 ggctctgttg tttcgtattt atgattcgtt gtatgtatgg ttgtaatacc attatgtgtg
    12661 ccaatcccaa taatattttg ttgctttaac atgtgaattt tatcgtcaat ttcaacaggt
    12721 aaactttgat caaaattcgc cgacatatca ttcgcaattg catttgcgct attatcatct
    12781 ttggctttag tcgcacgaac tttattgact gcttgttcaa tacgtttttg accaaacggt
    12841 ttcaaaatat agtctgtcgc atttaattca aatgcctgta ctgcgtattg gtcatgtgca
    12901 gttgcaaaaa taatcgcagg tggctctttc atcttttgaa ttttagctcc taattcgata
    12961 ccattttcat ccattaaatt gacatctaaa aatataatgt catattgatt tatcagtagt
    13021 gcttccaatg tttcttttac attttctgcc tcattaattt cttcaaaacc accaatttca
    13081 tttaataaat atgttaattc attacgtgct aatggctcat catctatgat taatgctttc
    13141 atatttattc ctcctcttgt ctttcataag gaagtacaca ccaaaaagtg gtaccgctcg
    13201 atgtcgattc aaattgtaat gctgcggatt ttccaaataa tccttttagg cgtaagttta
    13261 aattttctaa agcactacca gttccagatt ctgattctac agatgtttct cccaacaaat
    13321 gcattttatc tttagaaata ccctgaccat tatcttgtac aataatacgt acatgtgttg
    13381 cagtttcttt aatcactgac acgtcaatat cgttaccttg ctttcgattc gtaaacgcat
    13441 gtttgatagc attttcaact aaaatttgaa ttaaaaatgg tggtacaagc acattgcgat
    13501 atttgtcttc aacattaata ttgatattaa atcttcctgg ataacgtgct tgttcgagtg
    13561 ataagtatgc acgcacttga cttaactctt tatctaaagt aatcgtatgt tgctttgagc
    13621 cttgtaaatt cgctctgaaa aaataactca attctaatag taactctcgt gccttttcgc
    13681 tatttattct aactaaagct gaaatcgtgt taattgaatt gaagaaaaaa tgtggactca
    13741 cttgtgcctg taatgactta atctcagcat ctttcaataa cttactttgc gtttcggctt
    13801 caccaagttc aatttggcta ctaaaaatat ttgccaatcc ttctgcaagt tgacgttcca
    13861 caaaagttaa atcattaggg tttgtaaaat acatcttcaa tgtaccgacg atagaaccat
    13921 gcatctcaag tggtatcacg atagctgctc taagcgggca attcggatga ctacaaccaa
    13981 tctcttcttt agtatgaact tctttcaact tccctgattt caatacatct ttagacagac
    14041 ttgttaatat ttcatttgtt ggtatgtgat gatcactacc tgcacctaca tgcgataaaa
    14101 tttcattttt gcttgtaatt gctacggcag atactttcat taaattttta ataatcatcg
    14161 caatttgctg tgccgattct ctattcaatc cttctttaaa atacggcaat gtctggttca
    14221 tcaattgcag tacatcatgt gtttgaacag ccttcatttg ctcctcttgc tttaatgttg
    14281 aaatgataat agacataaaa atcgccgtac caacgctatt aacaataatc attggtagtg
    14341 caattaatga tatgaggtca accgcatatg ctttgtcgtg ggaaaatgtt aaaatgctca
    14401 acatttgaat catttccata acaattccaa tcatggcact ttttgcaata ctcgggtaac
    14461 gcttgcgtct ttgagcttgt aagccaaaat aaccagcaat tataccgata aatatagatg
    14521 atataagata aacttgtgca tccgccccac ccatatacac tctgaaaata cctgaaataa
    14581 cgccaacaaa tagacctaca aaagggccac caactaatcc tgcgacacct atcgttaata
    14641 cacgtgtgtt agctaaagat acatcatcat ctaaacggaa gtacacactt cctgacaaac
    14701 tatgttgatg atcgatgacg ataccagtta aattagacat taaggcaaac aaactgaaaa
    14761 taatacataa ttgccaacgt gctttccatg tacgtcgacg attcattaag tttttaaaat
    14821 atggaatatt catcaacaca taggccaaaa taataattaa acctacacgc tcaagtaata
    14881 acattgttag cgatagcacg atagcacctc agtgaattat aattatgttt attttatcat
    14941 aatagtgcat gtaaaattca tgaataaatt attaaaaaat cacgctacga tttaattatc
    15001 tacagcatta aaatttcata tcatttctta tactatattg atcatttttc ttttcaaata
    15061 atgatgttca tgtcactatt atgactttta tacatagcga aaaactgctg gacttatttt
    15121 tgccaacagt ttctaatcgc gttatttagt tatgatactt ttttaaataa tacgtgattc
    15181 tctaaatgta cgtgttcatg tgttaacact tcaagtgctt ttaatctctg ataaacgagt
    15241 cgccaagtac cacacgcttc tatcggaggt tcataactag atgttaattc gctcattttt
    15301 actaacaatt gtcccgttgc aatgtggtct gaaactaaat catctatcac agtattaata
    15361 tcgtctacta cctcaccttg ctcatattta atgagttttg gaaaatcgac atcgtcttct
    15421 ttttgcatat gctctaacat gccattttta aatgtatcgt atgtttcttt taactcaact
    15481 aaatatggat ggttaggtcc atgtactttc gataacttcg tcacataagg tgttaaattt
    15541 ttaaattctt ctcttagagg ttcatgatat gctgattgaa tatattgaat aagtgatgaa
    15601 acatttaaaa atttaggatt aagcgaacct ggtgtattcg tttgttcaac ttcattgaga
    15661 cgctgtaata attcgttcaa atctatattt ttcttttcta aggatgctgc ttctatactt
    15721 acttgtccgc cgcaacaaaa atctattccc acacttctaa aaatatccgc tgctttcgga
    15781 taatcagtta ctacatctgc tactatgtca tttttattta tcattataga taacctcctc
    15841 atttccagtg tatacaattt aatgtatttg aaaatgataa acgaggaaat gtcataatca
    15901 tgtcataaac agtaaaataa aaccctcaaa tcatgttagt tcacaatgat ttgaggggtt
    15961 ttattattta tttagtggat aagtgatatg tgcagtattt aacttaacaa tatttttgaa
    16021 gtctcgcttc ttaatatctt tgctaagtaa gatttgaact tcgttttctg atgtttggct
    16081 taacattccc atcaccatgt cgtcttttag cacgacacta gccatacctt cttcaaatac
    16141 ataaccacga tccagaccag ttaaaatgat tttgttttta ccttttaact tcattgagat
    16201 tgaatccgtt aatgtaatag ctgtaaagta taacgaaatg ttattaaagt cttttgtgaa
    16261 atacggtgtt aatctataaa aatcattatt caaatgaatt aatgcacttg tttctgcagt
    16321 ataagcatat ttattacgtt gattaccaac acgcacttca aatacttccg tacctatttg
    16381 gacttctaag aaaacatccc aaattttttc tttaggtaaa tacgctttaa tatcttctaa
    16441 cataatgtta aaacgatatt gtttattcgc attgacactt aaaggtacat acattgtgtg
    16501 cttcttgttt cgtgaatgta atttaagtct gacatcataa tgtccttcgc caatcataaa
    16561 ttcatttagc gttagctctg taacaatttt atgattatca aatttaaatg ctgtcatttt
    16621 cacttttggc ttagcgatat caatgccttc aaagtatggt tcgccttcat tgaattgaat
    16681 gtttaattta ccatctacaa tatcaaaacg gtagtattga ccttgacggt aactttcttc
    16741 tacagttcta tagttgtcta tatctttcgc tctcgcgtag tgcaacaatg gtcgtaattt
    16801 actcatcacc aatgcatcta cacgttctgg aactgcttgt ataaagtctc ctagagcatt
    16861 aatccattgt ggttgatctt ctagtttaac tttaagtgag aagccattcg tacgagaaaa
    16921 actaaaatga cgatttaaaa attctgctaa gatttgatct ttatgggctt cttctaaatc
    16981 tgcatttaat atttctacag caatcaatct catgacttcg taaaagtctt caggtgaaac
    17041 atacgcacta ctcatatgtt caccttcacg ctttgtagca taataatacg ctttatcact
    17101 taacacactg attcgatttg cattcaaata tgctttcatt gtaaataatt gatcttctgc
    17161 actctttaat tcttctggaa aataaatgtc attatctttt agtaatgctg ttctatagat
    17221 tttagtcggg cttaaagtat agataattct tgaatttaac aaagtaactt caggtgctgt
    17281 ttctttaaac atagattgtg gtacaccacg accattaaca cctttcattt taatcaataa
    17341 gacatctgag tgatgttcat caataaatgc tgctgcatcc tttaaagtct ctttgtttat
    17401 atagtcatcg gagtccacaa ataatacaaa ttcaccttct gcttgtttta acgccacatt
    17461 tctaggttta cctggaccac cagaatttgt ttcgagttga cttactttca agttcaattt
    17521 attgcgatac ttttcaacta tttgtaacgt ttgatctgtt gaacagtcat caactacaac
    17581 cacttcaaat tcagttttcg gaaaatcttg tttcgcaagg ctattgagta attctgttat
    17641 atacttttct gaattgtatg ttggaactat tactgaaaat ttcatcatta tacctctccc
    17701 actttgacta ctatataaac ttagctacca aataaatttc tgactaaacg ctcacttgat
    17761 cgaccatctt gatatttaaa atgtttatct aagaatggaa tgactttttc tccttcataa
    17821 tcttcattgt ccaaggcgtc cattaatgcg tcaaatgatt gcacaatttt acctggaaca
    17881 aatgattcat atggctcata aaaatcacgc gtcgtaatat aatcttctaa atcaaatgca
    17941 tagaaaatca ttggcttttt aaatactgca tattcatata ttaaagatga atagtcacta
    18001 attaataaat ctgttatgaa cagtatatca ttaacttctc taaagtcaga aacgtcaaca
    18061 aaatattgtt tatgtttgtc tgcaatatta agtctatttt tcacaaatgg atgcatttta
    18121 aataatacaa ccgcgttatt tttttcgcaa tatcttgcta aacgttcgaa atcaattttg
    18181 aaaaatgggt aatgtgctgt accatgacca ctacctctaa atgttggtgc gaaaagaatg
    18241 actttcttac ctttaataat tggtaattca tcttccatct cttgtttgat ctgtgtcgca
    18301 taagcttcat caaatagtac atcagtacgt ggaacacctg taggcactac atttttctct
    18361 ttaataccaa atgcttcagc gtagaatgga atatcggttt cagatgatac ataagctttt
    18421 gtatagctac gatgatttaa tgaatcaata aatggtccac cctttttacc agtacgacta
    18481 aagccaactg ttttaaaggc accaacggca tgccatactt gaataacttc ttgagaacgt
    18541 ctaaaacgca ctgtataaat caatgggtga aagtcatcaa caaagatgta gtctgccttc
    18601 ccaagtaaat atggcaatct aaacttgtcg atgatgccac gtctatctgt aatattcgct
    18661 ttaaaaacag tgtgaatatc atacttttta tctaaatttt gacgtaacat ttcgttatag
    18721 atgtattcaa agtttccaga catcgttggt ctagagtctg atgtgaacaa caccgtattc
    18781 ccttttttca agtggaaaaa tttcgtcgta ttaaatatcg ctttaaaaat aaattgtctt
    18841 gtattaaatg attgtttgcg gaaatactta cgtaattctt tatatttacg aacgatataa
    18901 atacttttaa cttccggagt cgttacaaca acatcaagga caaattcatt aacatcgcta
    18961 gaaatttcag gtgtaacagt ataaaccgtt ttctttgaaa tgccgccttt tctaaattct
    19021 tttaggtaag tctgcaataa gaaattgatt ttaccatttt gtgtttctaa ttcgttgtat
    19081 tcttcttctt gttctggctt tagattttga tatgcatcat taatcacatc tgggtttaac
    19141 tgtgcaatat aatcaagttc ttgctcattc actaataagt acttatcttc aggtaagtaa
    19201 taaccattat ctaagatagc tacattgaaa cgacaaacga attgattccc atctattttg
    19261 acatcattcg ctttcattgt acgtgtctca gttaaatttc ttaatacaaa attactatct
    19321 tctaaatcta ggttttcact atgtccttca acgaataatt gaacacgctc ccaatagatt
    19381 ttatctatat atatcttact tttaaccaac gttaattcat ccttttctat ttacataatc
    19441 cattttaata ctgttttacc ccaagatgta gacaggtctg cttcaaaagc ttctgtaaga
    19501 tcattaattg ttgcaatttc aaattcttga ccttttaaca acgctaattt atctacaata
    19561 tctgggtatt gaatgtataa gtctacaaca tcttggaaat cttttgaacc acttcgacta
    19621 ctaccaatca acgttagtcc tttttccaat acgagacgtg tattaacttc tactgggaac
    19681 tcacttacac ctaacagtgc aatgcttcct tctggtgaaa tataatcaat catttgattt
    19741 attgctgatt ggctaccacg cccaccaacg cactcaaatg catgatcaaa tgttaagcct
    19801 tcaggtattt tattaataaa gaagacatca tcaacaaatg agaagtgact caatttataa
    19861 tctgttttac caaagacata tattttagac tctggatata atttacgtaa taaaatggct
    19921 gtaatgtaac ctaagttacc atcaccccaa ataccaaatg tatttttatt tgaaatagat
    19981 ttcttttcaa aacgacgaat agcatgcaaa cttactgtta caagctctgt atatgaaata
    20041 atacttaaat caatatcatc aggtagtggt acagctctat catgatttag caacacaaaa
    20101 tcttgcataa acccatcatg tccacttgat ctgaagtagc tcgattttaa atagttttca
    20161 gcaatgacat cgtctttttc tgtcggtgta ttcggtacca taactacttt tgtaccttta
    20221 ttaaacacac ctttactgtc aaatacgacc tcaccaacac cttcatgaat taaagacatt
    20281 ggcaatttct gagataagac attctcatca cggctaccag tataatatct ttgatcggca
    20341 gcacaaattg acatatataa aggacgtaca atgacatagt cactgtaaat atctacgttg
    20401 ttatacgtaa cttcaaattg tctaggtgca actaattgat atacttgatt aatcatcggc
    20461 aataccacct cgaataatag cattcgctac ttttaaatcg taaggtgttg ttactttaat
    20521 gttatataac tcacctttta caagtcgaac cggtttgttt gtttctacaa taatcttaca
    20581 agcatcagat aaaatactct tttgctcatc actcaactgt gcatagcttt cttttaataa
    20641 attaatatta aacgattgag gtgtttgacc ttggtacatt tcattacgca ctggaattgc
    20701 atcaatcgtt tgattatctt tagatgtaac aatcgtatct atagcatcaa tcactgtatc
    20761 tactgcaccg tattctaaag cagcttgaat attttcttta ataatacgat gcgttaaaaa
    20821 tggtctaact gcatcatgtg tcacaatgac atcgtcatcg ttaataccat ttgttgattc
    20881 aatatgttta acgatattca taattgtatc gttacgatcg ctaccacctt gaatgacttc
    20941 aattctttca tcagaaattt tgaattttct aagtgtatct ttcgtatgcg tcatccattg
    21001 ttgtggcgtc gcgataataa ttttttcaaa atcattaatt aaaataaatt tttctaatgt
    21061 atggattaaa atcggtttgt tgtctaaatc taaaaattgt ttaggtaaag gtacgttacc
    21121 cattcttgag cctatacctc cagctagaat accagcgtat ttcataaaat acttcctcca
    21181 ttcaactata tctatattta attatttaaa tttcgttgca tttttcaatt gaaaactcat
    21241 tttaaaatca aaactctaaa tgtctgtgta ttacttaaaa ttatacatat tttgcttata
    21301 ttttagcata ttttgtttaa aactatatta cattatatca gacgttttca tacacaaata
    21361 ataacataca agcaaacatt tcgtttatta tttatatcac ttaactaatt aatttataat
    21421 tttttattgt ttttaagtta tcacttaaaa atcgtttggc aaattcgttg tgacgcgtgt
    21481 ccatcttcta atgaacagaa tttttgataa aataccgttc gtgcttcaat atactcattt
    21541 gcagtctcat cgatttgttt taatgcatca atgagtgctg tttgattttg aacaattgga
    21601 cctggcaact cttttttata atccatgtaa aaacctctaa gctcatcgcc atatttatct
    21661 aagtcatacg catagaaaat ttgcggacgc tttaatacac cgaagtcgaa catgacagat
    21721 gagtagtcgg taactaacgc atcgctgatt aagtataaat ccgaaatgtc ttcataatct
    21781 gaaacgtctt tcacaaaatc atcatgttca tcaatacgtg tcacaactaa ataatgcatg
    21841 cgtaataaaa tgacataatc atcatccagc gcttgacgca aagcttctat atcaaagtta
    21901 acattaaatt gatatgaacc ttctcgaatc gcttcatcgt cacgccaagt tggcgcatac
    21961 ataatcactt ttttatctaa tggaatattt aatcttgtct taataccgtt aatatattca
    22021 gtatcattgc gcttatgtga taatttatca tttcttggat aacctgtttc caaaatctta
    22081 tctcgactaa catgaaatgc atgttgaaat atcgatgtag aatatggatt aggtgacact
    22141 agataatccc aacgttggct ttctttttta aagccatctt ggtaattttg agtatttgtt
    22201 cctagcattt taacattact aatatccaaa ccaatctttt tcaatggcgt gccatgccat
    22261 gtttgtaagt acgtcgttcg cggtgatttg tataaccaat ctggtgtacg tgtgttaatc
    22321 atccacgctt tcgctcttgg catcgctaaa aaccatttca ttgaaaactt tgtaacatat
    22381 ggtacattgt gctgttggaa tatgtgttca tatccttttt ttacacccca tattaattgg
    22441 gcatcgctat gttcagttaa gtattcatat aatgctttgg gattgtcgct gtattgttta
    22501 ccatgaaagc tttcaaaata aattagattc ttgtttggca attttgatag taatttaaaa
    22561 gtcgtatata tactgtgttc tatcaatttt ttaattgtag ttttaatcat gtcgtacctc
    22621 cgacgtgttt ttgtaattat attaatatgt atgagcaagc tcattgtaac catgcctatt
    22681 atagcatttc agcataaaat acatttaacc attacacttg tcgttaatta tcatacgaaa
    22741 tacatgatta atgtaccact ttaacataac aaaaaatcgt tatccattca taacgtatgt
    22801 gttttcacat ttatgaatta gataacgatt ggattgatta ttttatttta caaaatgaca
    22861 attcagttgg a
//
LOCUS       DAKSQE010000032        22820 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.032, whole genome shotgun sequence.
ACCESSION   DAKSQE010000032 DAKSQE010000000
VERSION     DAKSQE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22820
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.032"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(280..684)
                     /locus_tag="O0V33_001098"
     CDS             complement(280..684)
                     /locus_tag="O0V33_001098"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCX8975745.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            complement(704..2116)
                     /gene="atpD"
                     /locus_tag="O0V33_001099"
     CDS             complement(704..2116)
                     /gene="atpD"
                     /locus_tag="O0V33_001099"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCX8975746.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            complement(2138..3004)
                     /locus_tag="O0V33_001100"
     CDS             complement(2138..3004)
                     /locus_tag="O0V33_001100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCX8975747.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            complement(3035..4543)
                     /locus_tag="O0V33_001101"
     CDS             complement(3035..4543)
                     /locus_tag="O0V33_001101"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCX8975748.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            complement(4565..5104)
                     /locus_tag="O0V33_001102"
     CDS             complement(4565..5104)
                     /locus_tag="O0V33_001102"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCX8975749.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLK
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELVKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            complement(5104..5625)
                     /locus_tag="O0V33_001103"
     CDS             complement(5104..5625)
                     /locus_tag="O0V33_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCX8975750.1"
                     /translation="MTETANLFFLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            complement(5825..6037)
                     /gene="atpE"
                     /locus_tag="O0V33_001104"
     CDS             complement(5825..6037)
                     /gene="atpE"
                     /locus_tag="O0V33_001104"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCX8975751.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            complement(6080..6808)
                     /gene="atpB"
                     /locus_tag="O0V33_001105"
     CDS             complement(6080..6808)
                     /gene="atpB"
                     /locus_tag="O0V33_001105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCX8975752.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            complement(6829..7281)
                     /locus_tag="O0V33_001106"
     CDS             complement(6829..7281)
                     /locus_tag="O0V33_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HCX8975753.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQY
                     IQYYLYLLIILGGLYLFTHHAFILGLIIGVTGSAINTVIFESYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYFNKSHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            complement(7345..8475)
                     /gene="wecB"
                     /locus_tag="O0V33_001107"
     CDS             complement(7345..8475)
                     /gene="wecB"
                     /locus_tag="O0V33_001107"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCX8975754.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLNE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENLGQPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVRDVAHKILGGHDRIELIEPLDVIDFHNFAKQSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVDAGTLKVVGTHEQDVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDFN"
     gene            complement(8499..9128)
                     /gene="upp"
                     /locus_tag="O0V33_001108"
     CDS             complement(8499..9128)
                     /gene="upp"
                     /locus_tag="O0V33_001108"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCX8975755.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            complement(9156..10394)
                     /locus_tag="O0V33_001109"
     CDS             complement(9156..10394)
                     /locus_tag="O0V33_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCX8975756.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALEMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            complement(10421..10945)
                     /locus_tag="O0V33_001110"
     CDS             complement(10421..10945)
                     /locus_tag="O0V33_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCX8975757.1"
                     /translation="MKDLTMLLHELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQFNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYQ"
     gene            complement(11052..11471)
                     /locus_tag="O0V33_001111"
     CDS             complement(11052..11471)
                     /locus_tag="O0V33_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HCX8975758.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
     gene            complement(11468..12514)
                     /locus_tag="O0V33_001112"
     CDS             complement(11468..12514)
                     /locus_tag="O0V33_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCX8975759.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKPGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAAGNHIIKGCEL"
     gene            complement(12598..13434)
                     /gene="prmC"
                     /locus_tag="O0V33_001113"
     CDS             complement(12598..13434)
                     /gene="prmC"
                     /locus_tag="O0V33_001113"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCX8975760.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLIKEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            complement(13421..14497)
                     /gene="prfA"
                     /locus_tag="O0V33_001114"
     CDS             complement(13421..14497)
                     /gene="prfA"
                     /locus_tag="O0V33_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCX8975761.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESSGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            complement(14498..15097)
                     /locus_tag="O0V33_001115"
     CDS             complement(14498..15097)
                     /locus_tag="O0V33_001115"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCX8975762.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            complement(15444..15698)
                     /locus_tag="O0V33_001116"
     CDS             complement(15444..15698)
                     /locus_tag="O0V33_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCX8975763.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            complement(15816..17132)
                     /gene="rho"
                     /locus_tag="O0V33_001117"
     CDS             complement(15816..17132)
                     /gene="rho"
                     /locus_tag="O0V33_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCX8975764.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            complement(17379..18806)
                     /locus_tag="O0V33_001118"
     CDS             complement(17379..18806)
                     /locus_tag="O0V33_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCX8975765.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADNVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
     gene            complement(19044..19379)
                     /locus_tag="O0V33_001119"
     CDS             complement(19044..19379)
                     /locus_tag="O0V33_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCX8975766.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
     gene            complement(19468..20727)
                     /locus_tag="O0V33_001120"
     CDS             complement(19468..20727)
                     /locus_tag="O0V33_001120"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCX8975767.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGA
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYRFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            complement(21193..22053)
                     /locus_tag="O0V33_001121"
     CDS             complement(21193..22053)
                     /locus_tag="O0V33_001121"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCX8975768.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            22271..22792
                     /locus_tag="O0V33_001122"
     CDS             22271..22792
                     /locus_tag="O0V33_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HCX8975769.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     ALDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAMALNYVYYDI
                     YTQMIEMTRDLEL"
BASE COUNT         6797 a         4562 c         3362 g         8099 t
ORIGIN      
        1 ggggccccaa caaagagaaa ttgtattccc aatttctaga gacaatgtaa gttggggtgg
       61 gacgacgaaa taaattttgc gaaaatatca tttctgtccc actcccattc ctatttttaa
      121 atacacatta gctgtgttta atgataaaga accactacat cataaatctt taatggttct
      181 ttatcaattc cttccccact ctcatgtttt gtatctttaa attaatataa ttcaaaaaag
      241 ggtcgaagat atgaaatcac atcttcgacc ctttatttac tattttaatt cagccacacg
      301 caaacggtta tttgctctct ctaaagctct ttcggctcta tgaatatcag tattgtcgtc
      361 atcattttcc aagtgagact ctgctcttgc tttagctaat ttagctcttt caacatcaat
      421 ttctcttgca gtttctgcag tctgaacaat gattgaaact ttatcttttc taacttcaac
      481 aaagccatcg cttacagcaa tatattcagt tccatcgtga aatttcactt ttacaaagcc
      541 tgtttttaaa gcagctacag ttggaatatg tccactcatg acacctatct caccagctgt
      601 tgtttgcata acaacgagtt caacattatc acgattgtaa acagaaccat taggagtgac
      661 aatatctagg tttaatgtat tcattatcca ttcctcctaa ttgttatact tcaacaccca
      721 tatctttagc ttttgcaata acatcatcca tgctaccaac taaacggaat gcatcttctg
      781 gaatatggtc atatttacca tctaagatat ctttaaagtt tgcaactgtt gtcttaacag
      841 gtacataaga acctttttga ccagtaaatt gttccgctac gtggaagttt tgagataaga
      901 agaattgaat tctacgtgcg cgttcaactg tttgtttatc ttcatcagat aattcgtcca
      961 tacctaagat agcaatgata tcttgtaatt cacggtattt ttgaagtgtt gattgtacat
     1021 cacgagctac ttcataatgt tcttgaccta caattgatgg ttccaatgct cttgatgtag
     1081 acgctaatgg atccacggct ggataaatac ccatttcagt taatttacgt tctaagtttg
     1141 tagttgcatc taaatgggca aacgctgtcg ctggcgctgg gtcagtatag tcatcggcag
     1201 gtacgaatac cgcttgaata gaagttactg atccttttgt tgtagacgta atacgttctt
     1261 gtaattgtcc catttcagta gcaagtgttg gttggtaacc tactgcagaa ggcatacgac
     1321 ctaataatgc agatacctca gaaccagctt gtgtaaatct gaaaatgtta tcgatgaata
     1381 ataatacgtc ttgaccttgt tcgtcacgga aatattcagc cattgttaaa ccagataatg
     1441 caacacgcat acgtgcacca ggtggctcat tcatttgccc gaataccatg gctgttttct
     1501 taattacacc actatcactc atttcgaagt ataaatcgtt accttcacga gtacgttcac
     1561 ctacaccggc gaatacagaa ataccaccgt gctcttgagc gatgttgtta attaattctt
     1621 ggattaatac tgttttacct acaccggcac caccgaacaa tccgatttta ccacctttaa
     1681 tataaggtgc tagtaaatct actactttaa tacctgtttc taaaatttga acttctgttg
     1741 aaagttcatc gaatgctggt gcttgacgat ggataggatc gcggcgaaca gaatcactaa
     1801 tttcttcttt aaggtcaatt gtttcaccta gtacattaaa tacacgacct aatgtttcat
     1861 caccaacagg tacactaatt tctttgcctg tatcttttac atccatgcct ctttggacac
     1921 catcagttga atccatcgca attgtacgaa caacgtcgtc acctaattgc agcgcaactt
     1981 ctaatgttag ttgtattgta ccttcttctt taggcacatc aataaccaag gcgttattaa
     2041 ttttaggaac ttcgttatgt tcaaatcgaa catcaattac aggacccata acttgagtta
     2101 cacggccaat tcccatgcta ttttcctcct ttaaatatta ttcaagcgct gcggaaccac
     2161 caacaatttc agtaatttgt tgcgtaattt ctgcttgtct cgctctgtta tattctaatg
     2221 ataagtcatc aataagttca gttgcattat cagtggcatt tttcatcgca gtcatacgtg
     2281 ttgcatgctc acttgctttt gcgtctaata ttgttccgta aatcaaactc tcaacatatt
     2341 gaggcaagat tacacttaag atagattctt tatctggctc aaattcataa gaagacaaat
     2401 gaccatgtcc cttactagaa tcctcttgag ataatggtaa tacttgtcta gatgtaggct
     2461 tgttttcaag aacgctgaca taatgactat agtatatatt taattcatca atttcttctt
     2521 cactgtataa gtctatagca tggttagcta gtgcttgaac agatttgaaa gaaggttgat
     2581 caggtacgtc tacttgagaa tactcaatgt cataacctct attttttagg aaatcaacac
     2641 cttgttgccc taaaactaca atactgtatt cgctactatc ttgatgtttc gcttcaatat
     2701 cagtaatcaa ttttttaagc acgtttgcac tatatgcacc tgctaaacct ttatcactcg
     2761 tgataactaa atatccactt ctagtaattt tacgaggtct tagcattgga tggtttgtat
     2821 tgctacttgc ccctgcaact gcagtaattg catcttgcat tttatccata tatggtgtga
     2881 attgttttgt atttttttca gctctacgaa gttttgaact tgataccatg ttcatcgctt
     2941 tcgtaatctg cttcattttt ttggttgatt ttattcgagt atctatttct ttaagagaag
     3001 ccactatctc accaccttat actaaacttg tttattattc agatttgcta aagctttttt
     3061 tgaattcgtt aattgctgtg tcaaacttct cagcatctgg taagccacca gtttctctga
     3121 tttcatttaa cagttcagta gcatttgatt ctgcccagtg gtttaactcg tcttcaaaac
     3181 gtgtgatatc tacaactgga atatcatcta aatatccttt tgttaatgca taaataatca
     3241 acacttggtg ttcaactggt aatggtttgt tttgatcttg ttttaagact tcaacagtac
     3301 gtttaccacg ttctaattta cttgcagtaa attcatcaag gtctgaaccg aattgtgcaa
     3361 atgattcaag ttctctatat gacgctaagt caagacgtaa cgtaccagca actttcttca
     3421 ttgctttaat ttgtgcagat ccaccaacac gagatacaga ttgtccggca ttaatcgctg
     3481 gtcttacacc tgagaagaat aaatcagatt gtaagaagat ttgtccatct gtaattgaaa
     3541 taacgtttgt tggtacataa gctgaaatat caccagcttg tgtttcgata attggtaatg
     3601 cagtaattga accgccacct aagtcatcgt ttaattttgc tgctctttct aataatctac
     3661 tatgtaagta gaatacgtca cctgggtatg cttcacgacc tggaggtcta cgtaataata
     3721 atgataactc acgataagct gctgcttgtt tagttaaatc atcatatacg attaaaacat
     3781 gtttaccgtt aaacatgaat tcttcaccca ttgttacacc tgaatatggt gcaatatata
     3841 ataatggaga aggttcagaa gctgatgctg ctacaacaat agtgtagtct aaagcgcctg
     3901 cttgtcttaa cttttcaaca tttgctctta ctgttgaatc tttttgacca atagcaacat
     3961 agatacaaat cgtaccttga tctttttggt tcaaaattgt gtcaattgca attgttgttt
     4021 tacctgtttg acggtcaccg atgattaact cacgttgacc tctaccaatt ggtactaaag
     4081 catcaattgc tttgatacct gtttgtaatg gctcatctac tgatttacga tccattacac
     4141 cagtagcttt tttctctact ggacgtgttt tagttgtgtt aatcggtcct tgtccatcaa
     4201 taggttgtcc taatggatta acaactcttc cgattagttc ttcacctact ggtacttcca
     4261 tgatacgacc agtacgttta acttcgtcac cttcagtaat acctgtgtat ggtcctaaaa
     4321 taaccacacc cacgtttgac tcttcaaggt tttgggctaa accaagtacg ccgttatgga
     4381 attctactag ctcaccagcc ataacgtcat ttaatccgtg aattaatgca ataccatcac
     4441 caatttgtaa tacagtacct acatcagtta cggacatttc tgactcataa ttttcaattt
     4501 gtgagcgaag taatgcactg atttcttcag cttttatggc catctatgtc actcctcttt
     4561 ataattaatt aactcttcta aattttcttt gtaattggac aagatcattt ctaacactac
     4621 catctaatac agttgtgccg actttaactc taaatccacc aattaaatct ggattaattt
     4681 ttgtatctac aataacttta gataacttcg tttgttgagt tactagtttg acaatcttaa
     4741 ctaactcttc ttgactcaat tcatatgttg actcaattgt tgcaaaatct tgattgtagt
     4801 gtccgttata taagctttgg aacgccttga atacgtcagc tattaatgag atatgtctat
     4861 tatctgctaa cacgtacatc atatttttaa tgtatggatt aatgtcagta aatacaccat
     4921 taatcaactc acgacgttgt tctgctgttt gagttggatt actgtcaacc attttcaatt
     4981 gctcaatttt atcttttact gcttcgttta taactgttaa ttcttcatta atagtttcta
     5041 aattatttgt atctaatgac acgtcaaata atgctttagc atacttgtta gctactttta
     5101 ccattattta tcgcctgcct cttttagata cttgtcaacc aatgcttttt ggtcttgttc
     5161 agaaatttct tttctaagaa ctttagaagc aattaacact gatagttcag atacttgatt
     5221 attaatatct gcaatggcac gttctttttg gctattgatt tcactttgtg ctgtttcaat
     5281 cataccgttt gcacgtacgt ttgcttcatg aataatttgt tcttgctgtt gacgtgcttg
     5341 aaccttagca tcttctaaaa tcttttgaac ttcttcttgt gtttctttaa gtttttgttt
     5401 attttcttct tcaagtttct gtgcatttaa cttagcttgt tctgcgtcat cgatatctct
     5461 gttaatatct ctttcacgtt tatccattac atctttcaat ggaccccatg cgaacttttt
     5521 aagtaacgca agtaatacga tgaaagttag gacctgtaca atcacagtac cccactcaac
     5581 gcctccagct gcaccaagaa agaataagtt agctgtttca gtcacgggtt catgacactc
     5641 ctttcattaa aataatcgct agtgttatat agaaaaaaaa cgacgaggtt aggacctact
     5701 gaatttatgc gataggcatg gtgcgttcat atctcaacta tttaataaag ttcaaagata
     5761 ttactatata tggcatcatg aattatgtcc tatgcgcttt gtcccgacct cttttatctg
     5821 ttaattatcc agcaaatgtc atgaatgcaa ttactacacc gatgataggt aatgcctcaa
     5881 ctaaacctac accaatgaac atgataccca ttaattgacc acgtgcttct ggttgacgtg
     5941 ctacaccttc aactgttctt gaaacgatta aaccgttacc gatacctgct cctaatgctg
     6001 ataaaccaat tgcgattgct gctgcgatta aattcataat ttaatttcct cctgtgattg
     6061 tatataatta ttgaaatttt tagtgttcat ctgccacttt atgtgacata taaaccatcg
     6121 aaagcataat aaagatatat gcttggattg ttcctacaaa tattgaaaat gcttgccaaa
     6181 caattaatcc tgggatacta ataatccaac cccatgctgg ttcgttaaag aataagccag
     6241 caagtaatgt taatagtatc tcacctgcaa atatgttacc gtacaaacgc agaccaagcg
     6301 ttaatgttga agtgaactct tcaaaaacat taataattgc caatggccaa aacggctgta
     6361 cataaccttt aagatattgt ttcgtaccac gcattttaat tccataaaag tgagttaaca
     6421 gtattatcgt tgtagacaac gttaaagtca ctgttgcatc agctgttggt gatttccacc
     6481 acaatgtgtg atctttcgtt actattgaaa acggaagacc taacatatta gctataaaaa
     6541 tgtacagaat cagcgttact gctaagaagt ggaattgacc accttttttc caagccatgt
     6601 taccttcaat gattcccctc acgaaatcaa aaatccattc aacgaaattt tgtttgccag
     6661 ttggtctttt tttcaaatta cgcgtacaaa tgatagcaag tagaaaaaca agaaacgccg
     6721 taacaagtat catcaatata cttgataaat tgaaaacgat atcaaaaccg aataaattcc
     6781 aactcacgag cggggatttg tgatccataa tctcacctct ttcttaattt atttgctcac
     6841 ctttagtaaa ggacgtatga taactacaac atatgaaatc attaaaccaa taattatacc
     6901 gattatactt acatgcgatt tattaaagta ccaaatcata caggcaataa ttgcaactaa
     6961 gtatcgccac atatttccag ttgaaatatg catagtgtct ggtcttttag ctttggctaa
     7021 gtagctctca aatataacag tattgattgc agaaccagta acaccaatga ttaatcctaa
     7081 gataaatgca tggtgtgtaa aaaggtataa tcctcccaat attattaata aatacaaata
     7141 atattgaatg tactgtttaa aaatgttgta gaaacgactc attttatatg cctccgtgtg
     7201 actgatatca atcatgaaaa ccgttccact catacaaact aaatgataat ataggcgatt
     7261 tgtggtgtca attctattca aattgttgta aaaaaataag catgttcaga cacaatttag
     7321 acacatttag acaaaaagtc ttttttaatt gaaatcgctt ggtttttcag tgattaaatt
     7381 caaataatac ttaatatgat taactattct ttctgaagca aatccatcac cgtaaggatt
     7441 agatgcttca gacatttgat gatataatct ctcatcatcg attaattctt ttgcagcttg
     7501 atatacgtct tgttcatgcg taccaacaac tttcaacgta ccagcatcaa caccttcagg
     7561 tcgttctgta acgcttcgca atactaggac aggcttatta aaagatggcg cctcttcctg
     7621 tatgcctccc gaatccgtca aaataaaata ggattgttta gcaaaattat ggaagtctat
     7681 tacatctaag ggttcaatca attcaattct gtcatgcccc cctaaaatct tatgagccac
     7741 atctcggact tttggatttt tatgcattgg atatactaac gctaaatcgg tgtactcatc
     7801 tatcaagcgt ctaactgctt taaatatatt ttccataggt tgacctaaat tttcacgacg
     7861 gtgtgctgtc ataagaatga actttttgtc atggtattta tccatgatgt tagatttata
     7921 attgtcatca actgtatatt tcatagcatc aatcgctgta ttaccagtga caacaacact
     7981 ttctgaatat ttcccttcat ttaacaaatg cgatgcagca tttttagtag gtgcaaaatg
     8041 taagtcagct aatacaccaa ctaattgtct attcacctct tctggaaaag gtgaatattt
     8101 atcataactt ctaagccctg cttcaacgtg tccaatcggc acttggttat aaaatgccgc
     8161 taaaccacct gcaaatgtcg tcatcgtatc accatgtaca agtaccatgt ctggtttttc
     8221 taattgaatc acttgttcta attgagtgat tgatttagaa gttatctcag aaagtgtctg
     8281 tcctgatttc ataatattca aatcgtattt tggtttgatt tcaaaggtac ttaatactga
     8341 atcaagcatt tctctatgct gtgctgtaac aacaacaatt ggctcgagca ttttttcttg
     8401 ttccaaagct ttaactaaag gagccatctt tatggcttcg ggtcttgttc caaatatggt
     8461 cataatcttt ttcatcaaac tacttatctc cgattcttct atttagtacc aaacaatcta
     8521 tctccagcgt cacctaaccc tggtgtgata tatgctttgt catttagctt ttcatcaagt
     8581 gcagcaatat aaatatctac atctggatgt gcttcatgca tcttttctac gccttctggt
     8641 gctgcaatta aacacatgaa gcgaatattt ttagcgccac gtttcttcaa tgaagtaata
     8701 gcttcaattg ctgatgcgcc tgttgctaac ataggatcaa caacaatgat ttgtctttca
     8761 gtaatatctt gaggtaactt agcaaaatac tctacagcct ttaatgtttc gggatctcga
     8821 tacaaaccga tatgtccaac tctcgctgca ggtactaaac ttaaaatacc atcagtcata
     8881 cctaaaccag ctcttaaaat tggaacgata gctaattttt taccagctaa tcgtttagcc
     8941 gtcattttag ttacaggcgt ttcaatatca acatcctgaa gctctaagtc tctagttact
     9001 tcatatgcca tcaacatacc aacttcgtct acaagttctc taaattcttt agtacctgta
     9061 tttacgtctc taatatagct tagtttgtgt tgaattaatg gatgatcgaa aacgtgtact
     9121 ttactcataa aaattactcc tatctttgtg tatgtttatt gatatagagg atattcagct
     9181 gttaatttcg caacgcgttc tttagcttgt tgtaattttt cttcatcttt actatttttc
     9241 aatgctaaac tgatgatttt tgcaacttcc tcaaaagcct tttcatcaaa tccacgcgtt
     9301 gttgcagcag gtgtacctaa acgtatacca ctcgttacaa aaggtttttc ttgatcgaac
     9361 ggaatggtat ttttgttaca tgtgatacca actgaatcta aagtctcttc agcttcttta
     9421 ccagtaagtc ctatagaccc ttttacatca acagctacta agtgattatc tgtaccacca
     9481 gaaacaattc taaatccttc attaattaat gcttctgcaa gaacttttgc gtttttaacc
     9541 acttgttgtt gatacgtttt gaaattattt tctaacgctt ctccaaaagc aactgctttt
     9601 gctgcaataa catgctcaag aggtccacct tgaataccag ggaaaattgt tttatctatg
     9661 tcttttttat attcttcctt acataaaatc ataccaccac gtggtccgcg taatgttttg
     9721 tgtgttgtag ttgttacaaa atcagcatat tctactggat ttggatgtaa acctgccgct
     9781 actaatcctg caatatgtgc catgtctacc attaacttag cgtttacttc atctgcgatt
     9841 tctttaaact ttttgaaatc aattgttctt gaatatgctg atgctcctgc cacaataagc
     9901 ttaggcttat gctctaacgc taatttacga acttcatcat aattgattcg ttctgtgtct
     9961 ttatctactc catattcaac gaaattgtag aatttaccac taaaattaac aggcgctcca
    10021 tgtgtcaagt gaccaccatg actcaaattc atacctaaaa ctgtgtcgcc catttctaat
    10081 gcaactaagt aaacagccat gttcgcttgt gaacctgaat gtggttgaac attgacatgt
    10141 tcagctccaa acaacgcttt agcacgatca attgcgatgc tttcagtaac atctacaaac
    10201 tcacagccac cataatatcg gcgtcctgga tagccttcag catacttatt agtcaacact
    10261 gaaccttgtg cttccataac cgcttccgat acaaaatttt ccgatgcgat taactctatg
    10321 ttgctatttt gtctctgaaa ttctctctcg attgcttctg cgataacttt atcttgcttg
    10381 gtgatataag acataaaatc tccccttctt tcaaaaaaac ttattggtat ttagcacgtt
    10441 cgccaccaat ctttttcggc ctagatgtgg caatagttac aattgcctgt cctacttgct
    10501 ttactgaagt ccttacaggt acacatacat gtttaatatg catgcctatt aacgtttgac
    10561 caatatcaat tccacaagga acagtaatat gttcgaccac gattggatcc ttcatatgct
    10621 gaaaagcgta tgttgccaaa ctccctccag catgtacatc tggaacgacg gaaacttctt
    10681 ccattgttaa tggattaaac tgagattttt ctattgttat cgctctgttg atatgttcac
    10741 atccttgaaa agcaaaagta acgcctgtct ctttactcac aacatctaat gcattaaaaa
    10801 tagtttctgc aacttccatc gaaccgacag tccctatttt ttcgccaatg acttccgatg
    10861 ttgaacatcc aattaaacat atatctcctt tattaaaaaa ggacatatct tttaattcgt
    10921 gtaataacat tgtcaaatct ttcataaaag cccacccttc ctaaaaataa aaaaggaata
    10981 tagcaaagtg ctacactcct ctattataac ttatttaact gttaacatat actaattata
    11041 cagaattcct actagcaaat aatatctttt aattttaaaa ttaaacttac aagttcttca
    11101 taggtatgta catacatttc ttttgtaccg ccgtatggat ctataacttc tcctgcttct
    11161 tttacatatt catgcaatgt gaaaacatga ttttgcaaac caaagtgtgc ctctattaat
    11221 tctttgtgcg aatacgacat agtcaaaata atatctgctt tcaaatctgc ttcagtaaat
    11281 tgttgcgata aggtcgtttc agctaaatga tgttcttcaa ctaagtcttc aacataattc
    11341 gaaacacctt gattgttcac agcgaatata cctcttgatt caaattgatg atttggcata
    11401 acctcttttg caatactttc cgctaatggg ctacgacatg tgttacctgt acaaacgaat
    11461 aaaatcttca tagttcacat cctttaataa tgtgattacc tgcagctttt aacatgcgat
    11521 tcataattgc ttctgtatta tcattcagct caaagccgta tatatacgcc gctgaaatat
    11581 tttcattttc atcaagtgaa tgtaacacat cataaagatt atgacttgct tgtttaacat
    11641 cattgtcatc ctgacataat tgaatgaatt gcgcttcact tggtataaac gccaccttat
    11701 tactcggcac aataaaagct atagaagacc aatctttacc gtcatttcca attttgctct
    11761 cgatatctgt aataattgta agtggtgtat tgggtgagta atgcttatac ttcatacctg
    11821 gtgcaattgg ctgttcagta tcattataat cagcatgggc gatactattc ggaagtattt
    11881 ctgttatcat tgctgctgtt atagaaccag gtcttgcaat tttataagga aaagatgtgc
    11941 aatctaaaac cgtactttct aatccttctt cactttgttc agcttgaaca ataccatcga
    12001 tacgtccatt caaatcttga tatacatgat tgaaagttgt tggtgaaggt ctaccactta
    12061 aattagcact tggagcagct agaggttcat ttatgatttg taataattgt ctacctacag
    12121 aatggcttgg cattctaaca gcaactgatg ataaacctcc agaaactttt cgacatagat
    12181 agcctggctt taacggcaat ataaacgaaa tagggcccgg ccagaatgcc tgcattaact
    12241 tttctacgcg tggatccaaa gtatatgtaa aatcttttaa ttgaccttta ctgtgtatat
    12301 gaacaataag cggattgtca gatggacggc ctttagcttc atatatttta gctacagctt
    12361 cttcatctgt cgcatttgct gcaagtccat aaactgtttc agttggtaaa cctattaaac
    12421 caccgtttaa aacaatgtct tttatttcat taattttagg atattgctgt aaatcttcat
    12481 tatattctct aacatcccaa attttagtat ccaacttaat cacgcctttc ttatttatca
    12541 taatacaaag caaaaagcta tgcacttaac taatcatagc aaaggcataa cttttaatta
    12601 ccatttaaat gagacgattc gatcgtggcc atttatatct ttaataatgt cgattttttt
    12661 gtcaggaaat ttatttaaaa ttattgattt aagtgcctca ccttgattgt aaccaatttc
    12721 aaaaacaact gggctgcctt tttccataac gtgaggtaaa tcttcaatga ttgattcata
    12781 aatagcatat ccatggttat ctgcaaacaa tgcctgatgt ggttcgaatc tcgtaaccgt
    12841 tggagacatc gtaaccatat ctttttcatc tatataaggt ggattagata tcaagccgtt
    12901 caacttgata ccttctttaa ttaagggctt taatgcatcc cctgttaaaa actgtatttg
    12961 tgattgatgc ttctcagcat tattacgagc catattcatt gcttcaagtg aaatatcagt
    13021 agcaataaca tttaaatccg gcttttcaca tttcaaagta attgcaagta caccactacc
    13081 cgttccgata tctacgattg ttgcatcatc ttctaactgt tgtaagaaat gcaacattac
    13141 ttcttcagtt tcaggtcttg gtatcaaaca atttgagttt acatcaaacg ttctaccata
    13201 aaatgaggca aagccaacta tatactgtat aggctctcct aataacatac gttgtaaggc
    13261 taagtcgaac ttcataatca tcgctttcgg catatcatca tgcatgtgga ctacaaagtc
    13321 cgtacgcgtc cattgaaata catctaacat taaccattca gctcgtgttt gttcaaaccc
    13381 tttttgttgt gttaaatgaa ttgcttcatc taacttttct ttataattca ccattattaa
    13441 gttctttcaa tttatctgtc tgctctgata aagtcagtgc atctataatt tcttctaaat
    13501 ggccttccat aatttgccct aatttttgaa gcgttagacc tatacggtgg tctgttacac
    13561 ggctttgtgg ataattataa gttcgaatac gttctgaacg atcaccagta ccgactgctg
    13621 atttacgttg tgacgcatac ttttgttgtt cttcttgaac tttcatatcg tataaacgtg
    13681 cttttaacac tttcattgct ttttcacggt tttgaatttg agacttctca gaagatgttg
    13741 caatgacacc agttggtaaa tgggtaatac gtactgcaga gtcagttgtg tttacgtgct
    13801 gaccacctgc accacttgaa cgatacgtat cgatttttaa atcttcattt ctaatttcaa
    13861 tttctacatc ttcaacttct ggtaaaactg ccactgtagc tgttgaagta tgaatacgtc
    13921 cacctgattc tgtttcaggc acacgttgaa cgcggtgcgc accattttca aatttcaatt
    13981 tactatacgc gccattacca gaaactgaga aactaatttc tttgtaacca ccatggtcac
    14041 tttcagacgc ttctactatt tcagttttga atccttgtga ttctgcatac tttgaataca
    14101 tgcgcattaa atcaccagca aaaatcgcag cctcatcacc accagctgct gctcttattt
    14161 ctacaataac gtctttgtca tcattaggat ctttaggaat caataatatt ttaagctctt
    14221 cttcaagatt tggaagttca gctttaatac cactactttc ctcttttaac atttctactt
    14281 cttctttatc atcagtttca cttaacattt cttcaatatc agctaattct tcttttttag
    14341 ctttatagtt acgataaaca tctacagttt tttgtaaatc agcttgctct ttagaatatt
    14401 tacgtaattt atctgaatca tttacaacat ctgggtcact taacagttca tttaactgtt
    14461 cgtatctttc ttctacaata tctaattgat caaacactta taattcctcc ttattattat
    14521 cactaggtgc tacgatatgg tgcgcgcgac aacgtggctc ataactttca ttggcaccta
    14581 ctaagataat cggatcatcg attttagctg gtttaccatt tattaatcgt tgcgttctac
    14641 tagatgaaga accacaaaca gcacaaactg cttgaagttt cgttacttgt tcactgacag
    14701 ccatcaattt aggcattggt tcgaacggtt cgcccctaaa atccatatct aatccagcaa
    14761 caataacacg gtgtccatct gctgatagtt tttctactat acttacaatt tcatcgtcaa
    14821 aaaattgtac ttcgtctatt cctataacat caacattagt taagtcgtgc gtcataattt
    14881 cacttgcttt agaaatatta atcgcttcaa tggcattacc attatgagag accacttttt
    14941 ctttatgata tcgatcatca atcgccggtt taaatacaac gactttttgt ttagcgtata
    15001 tgccccttct taaacgtctt ataagttctt cggatttacc gctaaacata ctacctgtaa
    15061 tacattctat ccaaccggaa tggtaagttt catacattga gagttccacc tttttcaaaa
    15121 cataatcgct ttattatatc atatttcaaa tattcataaa tgtctttttc ataattatat
    15181 cgatattgta catgaacaat tattttataa atttgtcttg catatttata tacagccatt
    15241 ctattttgtt gcttcttatt cgtgcgatgt ttctactcaa tacaaacttt aataaaggga
    15301 aaaatttatc attatgtatt aacaacatgt tattgagtca cttctttgaa cacagtcgta
    15361 agtaaatacc cttactacac ttataaataa aaaagctgtc tacaattcca ttataggaag
    15421 cagacagctt aacgattact atattagttg tttgatttaa gaccaaactt tttgttgaaa
    15481 cgttccacac gtccatccgc agcagcgaat ttttgacgtc cagtgtagaa tggatgtgaa
    15541 tcagatgaaa tatctaaacg aataactggg tattcttttc catcttccca ctccatcatt
    15601 tctgaagatg tttttgtaga accgcttaag aatttgaagt ttgtagtagt atctaaaaag
    15661 attacttggt gatactcagg atgaattcct tgtttcatta ttttcagctc ctttgccctg
    15721 aaccatctgg aacagagtta tttgtgagtt tttacccaat actgtgtaat tataaaggta
    15781 attaattcaa aacgcaagcc cctatataat gtttattaaa ttataggtcg acccgtttta
    15841 gtactttctt ctgcagactt ttgtagctgc ttgaagaaat cttcattatt cttagacctt
    15901 ttaagtttgc gaataaatct ttcagtaaag tctgttgagt cagtgaatag atttcttaat
    15961 tgccataatg tgtctaattc agatttactt atcaacaatt cttctttacg cgttgaactt
    16021 ctgccaatat caattgcagg gaagatacga cgttcagaca atttacgatc taaatgtaac
    16081 tccatgttac ctgttccttt aaattcttcg taaatcatat cgtccatacg tgaacccgta
    16141 tcaactaatg cagttgcaag tattgttaaa cttccacccg cttcaatatt tctcgctgca
    16201 ccgaagaatg cttttggttt gtgtaaagat gcaggatcta aaccacctga taatgtacga
    16261 ccacttggtg gaataactaa gttataagcg cgtgctaatc tcgttataga atccattaaa
    16321 ataatgacat cttccccaat ttctactaaa cgctttgcac gctcaagtaa taattcagct
    16381 actttaacat ggtgttctgg tggttcgtca aacgttgaat gaacgacttc agcagcttct
    16441 actgagcgtt ctaaatccgt tacctcttca ggacgctcgc caactaacaa aataaatagc
    16501 tttgcatctg gtttgttcgt actgattgca ttcgctattt cttttaataa cgatgtttta
    16561 cctgctttag gtggcgccac tattaaacca cgttgaccta aaccaatcgg tgttactaaa
    16621 tccatgatgc gcgttgaata attttgtgtt tctgtctcta atttaatacg ctcatctgga
    16681 taaagtggtg tcaaagcttg gaaatgcggt cgtttcttca cttcttctgc gttatggtca
    16741 ttgacaaagt caacttgtaa taagccataa tatttttcgt tatctttagg ttttctaact
    16801 ttcccagtta ctttatcccc acgtttaatt tcaaaacgac gaatttggct agcagatata
    16861 taaatatctt tttccccttt agaatagttc actgttctta aaaaaccata accatccggt
    16921 tgtatatcat ctaagatacc ttccatataa tagttaccat ctttttccat ttgtgcttcc
    16981 ataatagcta gaacaagttc ttttttattt aatttactat agttcgtcaa cttaagagtt
    17041 ttagcttttt gagtgagttc cttggtagta tagttcttgt acaattcgtg gaatgattca
    17101 tactgaggag atgtacgttc tctttcaggc atatagttta cacccatttc attatttttt
    17161 agttagtaag taatagatag gcatgacaat gcataaataa ctgtgctagc acaatcccat
    17221 tatgctaatc cccaaccttt taaatacatg aataactata gcaaattttt attttaatta
    17281 caaaatctat ttataaaaat tgcaaaatta aaacagtaca caaaacttaa ttagtcatgt
    17341 gcactgtttt attgcgattt ttaattcaaa aatcaaattt atttaaaata gccagctata
    17401 gatttcactt ctaagaactc ttcaatacca taatcgcccc attcacgacc taaaccagat
    17461 tgtttatagc caccaaatgg taagtctggc tttctacctg cttcgtttat ttctactgta
    17521 cctgcttcaa tagaacgagc tactttatgc aatgtttctt tgtccttacc aataacatat
    17581 cctgctaaac catattttgt atcatttgca atttgaatcg cttcatctaa atcgttataa
    17641 gtgataactg acattactgg cccaaaaatt tcttcttgtg ctatcgtcat ttgattatct
    17701 acattaataa aaattgtcgg acgtgcaaag tatccttttt caagtccttc tggtttacca
    17761 ggaccaccat aaaataattc agcaccttct tcaatacctt tattaatata attttgtact
    17821 tgatcaaatt gttttttact aatgataggg cctacttgtg taccatcttc tcttggatta
    17881 ccgacacgca cttggctaaa ttgttctttt aattcagcta agaatgcatc tttaattttg
    17941 ttaggcacta aaacacgtgt accagctgta catacttgac cagtattatt aacaactttg
    18001 cctgttgttg ctttagccgc ttctttaata tctacgtcat ctaggacgat gtatggtgat
    18061 ttgccaccaa gctctaatga taccttttta aaatctttag cagctttttc cataatttta
    18121 gaaccagtag ggcctgatcc tgtaaatgac atcatgcgta ctttaggatg ttcagataaa
    18181 ggattcccaa cgcctgcacc atcaccatta acaaggttaa atacaccttt aggaacaccg
    18241 actttatcaa aaatctcagc taaaataaca gctgcaaatg gtgtttcttc agatggttta
    18301 agtacaactg gactaccagc cgcaaatgct gctgctaatt ttaatgatgt ttggtttgta
    18361 gggaagttcc acggtgtaat taagccagat acaccgattg cttctttaac aactaaatca
    18421 tctccgcggc gttcttcaaa ttcgtaatta tctaatgcgt ctctcgctgc aacaaaatgg
    18481 tttagtccca tttgataatg gacacgctca gataatgata aaggagcacc taactcatcc
    18541 gtaatagctt gtacaatatc gtcttttctg ttttcatatt cttttacaat tttatctaat
    18601 aacgcttgtc tttctttcac agatgtatga cggaactcta aataaacatt gtctgccgcc
    18661 tcgacggctt tatcaacatc agctttatta cctttagcaa ctttcccgat tacttcttcg
    18721 gttgctggat ttataacttc tatcgtttca ttactattac tttctaccca ttcgccatta
    18781 atgtattgct ttgtgtagtc tctcattatt ctttcactcc tcaagaatga tttatatatt
    18841 atttatccgt aatgcatacg atataaacat tacattccat tatgatacgc tcccttttaa
    18901 tatgttcaaa ataaaatgct ttaaaatttt gctgcatcta attttttata tttttaaata
    18961 aaaataacta ttcccacatc atcgcaaata atgcgaagac ttagaaatag ttataatcaa
    19021 tgctatgttt aaacgtcatg taatcattta gcagtacgtt gatctgttaa atcgacatat
    19081 gattgcgccc atgcttcaat tggatgtaaa gcctctgcta acgctttacc tttttcagtt
    19141 agcacataaa taatttgtac tggactcgta gaaatgattt gcttctcaac taactcccat
    19201 tgtgcaagct ctgacaactt aagacttaaa gcacgtggtg ttattgtttt caaatctctt
    19261 ttcatatcgg aaaagtgtgc tgaacagtca ttacatcttg agagataatt aatgattaat
    19321 ccattccaac ttctaccaag tattttaaaa gtttcttcga gatacggaca tacttccatc
    19381 atcttcacct ctcatcaata aatttagaat gtaaacgaac accctttttt aatcagtata
    19441 attattatac cacaaaacaa gataaatcta tacagtttcc gtccaaatat ctgcacctaa
    19501 agcttttaaa tgttctacaa tatctgtata acctctataa atatgtttaa cattgtaaat
    19561 tgtagttaca ccctcagcaa ttaaaccagc aataattaaa caagctcctg ctcttaaatc
    19621 gctagcataa acttcagcac catgtaatgt tgatggtttt atcgttgctg tgccttcgtc
    19681 aacttcaata tttgcaccca tgcgctttaa ttcttcaaca tgtttaaaac gctccggata
    19741 aatcgtatca gttacaaatg aaggaccatt tgccataaat aataatggtg taataggctg
    19801 ttgcaaatca gtagcaaaac ctggatatac tagtgtttta atatcaacaa atcgatatgg
    19861 cgcattatta ttgatgcgaa ttctttcgtc tcttacatca acattcacac ctaattcact
    19921 aaatttagca gttaatgttt ctacatgttt cggaacaata ttatttaata taacattttc
    19981 tccacatgct gcagcgatac acatatatgt gcctgcttca attctatcag gtataacttg
    20041 atactcagaa ccatgtaatt ctttgacacc attgatttta attgttgatg tacccgctcc
    20101 cttaatatta gctcccatac ttgttaagaa gttagcaaca tcaactactt ccggttcttt
    20161 agcagcattt tcaattacag tttgtcctgt tgcataaact gcagctagca taatgttaat
    20221 tgttgcacct acgctaacca tatctagaaa aatatgtgca ccttttaact ctttagcttc
    20281 gattttcatt gatgtcgtac ttgattcatc aatttcagca cctaaagctt taaatccttt
    20341 aatatgttga tcaattggac gtggcccaag tggacatcct cccggtaaac caatcacaca
    20401 ttttttaaat ctacctaaca tggcacccat catatagtat gaagcacgta atgattcaac
    20461 tttattattt ggtaatgcag cattttgtat ttcagttgta tcaacttcta attccgtacc
    20521 atttagtgat gccttaatat ttaaatcttc taataaactc actaaagttt taacatcaga
    20581 gatttgcggt aacccttcta atttcacatg tccttgagct aataatgttg caggaatgat
    20641 agctacagca ctgtttttcg cgccactgat attaacttcc ccatttagtg tgcgtccacc
    20701 tcttattttt attacctctt gagccatatc atttatctcc tttgtccatt actttttaat
    20761 tttttgcatt attagcatta taaattaatt ttactttatt tgtaaaatag tttattttaa
    20821 tcgttaaaat cttgtctttc tattttcgat tgattaataa caaatcattc acctttacta
    20881 atatatgctg ttcttcccca cttgaccatg gtctaaagtc tgattattca actaaatatt
    20941 gacgttgttt tgacaattat ttagttttaa ttctacggtt ttcacgtaaa cttttttcat
    21001 aaattgctca ttttccttat aattatatca caatcaattt tgatatattt taccattttt
    21061 acgctttcat tttaaaatca aactgtttta tttattactt atacaacata aaaaagaagc
    21121 aaacaccatt ttgtctgctt cttttaaaat taatatccct acgttattaa taacttaaag
    21181 actaaatatt aattatttag cgcggtttga agtaccgaac tctttaattt tacctttaac
    21241 tgtttctttg atagcttcac gtgcaggtcc taagtattta cgaggatcgt aaacttcttt
    21301 gtcgttattt aaaacgtcac gaactgcttt tgctgaagcg atttggtttt cagtgtttac
    21361 gttaatttta gctgtaccaa atggaattgc tttttggata tctttagtcg ggataccagt
    21421 accaccgtgt aatactaatg gtaaacctgt agataaaccg atttcttcca tttctttaaa
    21481 tcctaatttt ggttcacctt tgtatggacc atgaactgaa cctaatgctg gcgctaatgc
    21541 atcaatacca gttttttcaa ctagttcttg acattcttta ggatcagcat aaatgatgcc
    21601 gtctgctaca acatcatctt cttgtccacc aacagtacct aattcagctt ctacagaaac
    21661 acctttttca tgagcgtatt caacaacttt tttagttgtt gctacgtttt cttcgaatgg
    21721 gctgtgtgaa gcatcgatca ttactgatgt gaaaccagca tcgatagctt ctttacattt
    21781 ttcaaagctt gaaccatggt ctaaatggat tgctacagga atagtgatgt ttaagtcatg
    21841 cattaaccct tcaaccattt taacaattgt gtagaaaccg ctcatgtaac gagcagcacc
    21901 ttcagaaaca cctaaaatta caggtgcatt ttcttcttgt gacgcttcta aaattgcttg
    21961 agtgaattct aggttattaa tattgtattg acctaccgca taaccatttt cttttgcatc
    22021 aattaacatt tctttcattg aaactaaagg catgaaagtt cctccttgag tgcttacgca
    22081 cgtatttttc aaagtaatta tatcaaaaaa atagcgtcaa tgcattgaaa tactgcagat
    22141 taaatgtgta ttttttgaaa aatgcgatag ttatactaaa tgcaaacgta tacattttta
    22201 tacaatttaa gccgttgcat aatatgtagt cattaattac aatattttat aagtagattg
    22261 gaggataaag atgtcaaaaa tcttaaacac acaactaata ggtatcttta ataggctaga
    22321 aaaacaatct ttagaaattc aaatggccgc ccaatgttta attcaagcaa ttggtggcga
    22381 aggttatgtt tatgtaaaag gttatgatga tttacagttt ttcgaaagtt tcattctaca
    22441 tagtgatgag cgtttaaaat ctagccgaaa acttgacgct attaaagact ttaaagaaat
    22501 tgactcaact gatcgtgtat tattattcgc accattttat aatgatcaag ttgcattaga
    22561 tattcaaaaa cttatcgcct tagatatcga tgtggtctta ataagcaaca aacctaaaac
    22621 agatgatttc cctgatcatc tcgtacattt tatcgattta tctacaccaa gacctatagt
    22681 ctatactgaa gattacgata agattgtcca gccacatgcg atggcattga attatgtata
    22741 ctatgacatt tatacacaaa tgattgaaat gacaagagac ttagaattat aaaagtcact
    22801 attgttattt aataccttgt
//
LOCUS       DAKSQE010000033        22644 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.033, whole genome shotgun sequence.
ACCESSION   DAKSQE010000033 DAKSQE010000000
VERSION     DAKSQE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22644
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.033"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..532
                     /locus_tag="O0V33_001123"
     CDS             <1..532
                     /locus_tag="O0V33_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975770.1"
                     /translation="QRNLFALQMNPVIFFIINFILISYLYDKQYVQFQAITGMSLFYC
                     LVIFPMTIVVYLRVSQKNYLRSNKIEMVMGTIIAIISLLLIILQAFNITWGVIPITNF
                     GHQFFFFIGIILVIAGIFYKRLEFSGIGLLFCQKTVDAMIHNPQSAQTFSLIIWILLV
                     VLVIYFTIRLSSRTRL"
     gene            701..1489
                     /locus_tag="O0V33_001124"
     CDS             701..1489
                     /locus_tag="O0V33_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCX8975771.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVITYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDK
                     NHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFLSYYLNFLKSVS"
     gene            complement(1739..2113)
                     /locus_tag="O0V33_001125"
     CDS             complement(1739..2113)
                     /locus_tag="O0V33_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCX8975772.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
     gene            complement(3061..3990)
                     /locus_tag="O0V33_001126"
     CDS             complement(3061..3990)
                     /locus_tag="O0V33_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCX8975773.1"
                     /translation="MLLFYIIGVLVGMLIPIQTSVNSRLSLYTKSPFYTSFISFSVGT
                     ICLIVLNIFINPDVFSLHFFSNQTFNYTWFVGGLLGVGYLTGNLLLLPKLGATLTVIA
                     TVAGQIIMGVIIDTFGLFGATIHDFNLIKAIGVLLLIIGIVIMNQFNKNNLLLTDQKY
                     LLFWLLLGFIFGFFPPIQTTINSLLASHTHSPAFASLVSFTIGSITLLILTAIFNRSL
                     KLKTSHLKFGKLKPIYFTGGILGMAFVTSNIILMPHLGAALTTLIGMFGQILMGILID
                     HFALFGSPKIAMTSRKTIGLLCILTGIIFLRLF"
     gene            complement(4183..4407)
                     /locus_tag="O0V33_001127"
     CDS             complement(4183..4407)
                     /locus_tag="O0V33_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HCX8975774.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNIVTEQVARDSMNALVDLN
                     ILKSNSGAITKVHSAQIIDKTTTVLFEAKK"
     gene            complement(4424..4627)
                     /locus_tag="O0V33_001128"
     CDS             complement(4424..4627)
                     /locus_tag="O0V33_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975775.1"
                     /translation="MSKTNHITIVLSFTKLDTNGKQTEFKRRFANINPEVSNDQIKTF
                     SKLIERLTGETYNNIEVIKSVSI"
     gene            4782..5342
                     /locus_tag="O0V33_001129"
     CDS             4782..5342
                     /locus_tag="O0V33_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCX8975776.1"
                     /translation="MNKVEVIKCNDDINKMYDALKARSDRDYLFFKLAIHSGMKITEL
                     LTITVEDTKRLIEKGTLSELCKAHYHSLIKIRLPETLSNELLHYIENKSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQAAIKAGIENVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            5361..7763
                     /gene="mnhA2"
                     /locus_tag="O0V33_001130"
     CDS             5361..7763
                     /gene="mnhA2"
                     /locus_tag="O0V33_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HCX8975777.1"
                     /translation="MSLVYLLIAILVIMAMILLTSKRRAMAKYAGYIALTAPVIASIY
                     FLLQVPSVIKQHYLSVSIPWMTSLDINVDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYLYLTLFMFSMLGIVLADNTILMYVFWELTSVSSFLLISYWYNNGDSQ
                     FGAMQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHALFIPMIIMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYIYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFAALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMMIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIIIVCMGVIASIFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVPALRGV
                     SGGNHQIDPLVPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     GYEMVYRQFEKFTTKRFKRVMQDRLNQYIIMTLGIFMVIIGYGYIRIGLPKVHQLHVS
                     EFGPLEVILAIVTVIIGLSLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSKVNKKREIIKISVSLMMALIVVSLIFIAQQADGLAS
                     ISNFYLRADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            7750..8175
                     /gene="mnhB2"
                     /locus_tag="O0V33_001131"
     CDS             7750..8175
                     /gene="mnhB2"
                     /locus_tag="O0V33_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HCX8975778.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIMPTFFGKPFLSQYETTLT
                     LPILGHIHVTTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            8172..8516
                     /gene="mnhC2"
                     /locus_tag="O0V33_001132"
     CDS             8172..8516
                     /gene="mnhC2"
                     /locus_tag="O0V33_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HCX8975779.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSNKSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            8506..10002
                     /gene="mnhD2"
                     /locus_tag="O0V33_001133"
     CDS             8506..10002
                     /gene="mnhD2"
                     /locus_tag="O0V33_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HCX8975780.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWTAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNGLIHPLLV
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPFFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVHFKKIPQYRKG
                     ILSILVVVVIAIGIAAPVLLKITNDATELNTNDQLYQKLVNPHLKGEE"
     gene            10003..10485
                     /gene="mnhE2"
                     /locus_tag="O0V33_001134"
     CDS             10003..10485
                     /gene="mnhE2"
                     /locus_tag="O0V33_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HCX8975781.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDDFYVRKIWVAIKFLGVYLYQLITSSISTINYILFKTKDMNPGLLSYETRLTSDW
                     AITFLTILIIITPGSTVIRISQDSKKFFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR
                     "
     gene            10482..10784
                     /gene="mnhF2"
                     /locus_tag="O0V33_001135"
     CDS             10482..10784
                     /gene="mnhF2"
                     /locus_tag="O0V33_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HCX8975782.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGYVFNGNNKRNL"
     gene            10759..11196
                     /gene="mnhG2"
                     /locus_tag="O0V33_001136"
     CDS             10759..11196
                     /gene="mnhG2"
                     /locus_tag="O0V33_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HCX8975783.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDDHTHASILLSSNEQNSTKALQLRAKKREEYRKKWYQND"
     gene            11536..13578
                     /locus_tag="O0V33_001137"
     CDS             11536..13578
                     /locus_tag="O0V33_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCX8975784.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
     gene            complement(13664..14812)
                     /locus_tag="O0V33_001138"
     CDS             complement(13664..14812)
                     /locus_tag="O0V33_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCX8975785.1"
                     /translation="MSNIKIVTLIGFFTMFIVGVSDLIVVGMLNEMSKEFHVSNALIG
                     QLVTLYAVAFSILSPLLTKVTSQINDKKLLVSCLILFIIFSMLTTVMTNYFFLCIVRI
                     FMAGIASLITVKLMSTGANIVPVEYKASVIANIYVGFSAANILGIPIGTLIASHYNWK
                     IPFYIIAIITFLCLLGIMLFIPNKSHYSINNHSKYQLVNFRGTLIMLGFLLIMMISNS
                     MLFTYIEPLVKSKGHSIWIVSLCLFFSGIAGVLGSKLGNNLTEKKGYRIAGNIIIIVY
                     IISIMSILLTGQNVILLILSIFTWNLFHWGTNPTVQYALLKFLKGDPSQILSYDISIL
                     NLGIGLGSLIGGILFSIDNQFNLSIVVAMILAMVSSILLKTAVHTEKN"
     gene            complement(14816..15314)
                     /locus_tag="O0V33_001139"
                     /pseudo
     CDS             complement(14816..15314)
                     /locus_tag="O0V33_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            complement(16454..17383)
                     /locus_tag="O0V33_001140"
     CDS             complement(16454..17383)
                     /locus_tag="O0V33_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCX8975786.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
     gene            complement(17380..18216)
                     /locus_tag="O0V33_001141"
     CDS             complement(17380..18216)
                     /locus_tag="O0V33_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCX8975787.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            complement(18210..18953)
                     /locus_tag="O0V33_001142"
     CDS             complement(18210..18953)
                     /locus_tag="O0V33_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCX8975788.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLHNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            19075..19719
                     /locus_tag="O0V33_001143"
     CDS             19075..19719
                     /locus_tag="O0V33_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCX8975789.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            complement(19799..20551)
                     /locus_tag="O0V33_001144"
     CDS             complement(19799..20551)
                     /locus_tag="O0V33_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HCX8975790.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYFMPPIMLLIGLASSHYQIPSFFIFTYLLIFIYFLILTSRKGSPIVVITLISIM
                     LYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKE
                     LSHVPIFIYSTIWIIFNLYAVNILLKYTHLIN"
     gene            20787..21551
                     /locus_tag="O0V33_001145"
     CDS             20787..21551
                     /locus_tag="O0V33_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HCX8975791.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVGEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIEL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESKVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
     gene            complement(21611..22405)
                     /gene="tagH"
                     /locus_tag="O0V33_001146"
     CDS             complement(21611..22405)
                     /gene="tagH"
                     /locus_tag="O0V33_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HCX8975792.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDNIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDESRFVIK"
BASE COUNT         7683 a         3227 c         3714 g         8020 t
ORIGIN      
        1 tcaaaggaat ttgtttgctt tacaaatgaa tccagttatt ttctttatta taaatttcat
       61 tctcatatcc tatttatatg ataaacagta tgttcagttt caagctatta ctggaatgag
      121 tctattttat tgtttagtga tttttccaat gactattgtt gtatacctaa gggtgtctca
      181 aaagaattac ttgcgtagta ataaaataga aatggttatg ggtacaatta tcgctatcat
      241 atccctgtta ttaattatat tacaagcatt taatattact tggggcgtta taccaattac
      301 aaattttgga catcaatttt tctttttcat tggtattatt ttagtaattg ccggcatatt
      361 ttataagcga cttgagtttt cgggaatcgg cttattattt tgtcaaaaaa ccgtagatgc
      421 aatgattcat aatccacaat cagcccagac tttttcatta attatatgga tattattagt
      481 agttctagtt atatatttca caattagatt atcttcacgt acaagattat aaatatgata
      541 aaactattca cttgattaat tgtattaatt gagatgaata gtttttttat tgttggaata
      601 acttttggta atttataaat aatttaaaaa aattgtttat aaaatggaag cgtacataga
      661 atgaaggttg ggtatatagt ttattgaggg aggtgtcaca atgaataaag tcacaattaa
      721 tcctcaaatc caattaactt atcaaattga aggtaaaggg gatcctataa tattacttca
      781 tggattggat ggtaatttag ctggatttga agatttgcaa catcaactag catcatcata
      841 taaagtaatt acctacgatt taagaggtca tggcaagtct tctaaaagtg aatcatacga
      901 tttaaacgat cacgttgagg atttaaaaat tttaatggag aagttaaata ttcatgaggc
      961 acatattcta ggacatgatt taggtggggt agttgctaag ttatttacag ataaatatgc
     1021 ttatcgtgta aaatcattaa ctaccattgc atcgaagaaa gatgacttaa tacacagctt
     1081 tactcaattg ttaattcaat atcaagatga tatagcgggt tttaataagt ctgaagcgta
     1141 tattctttta ttttctaaat tgtttagaaa tcaagagaag acgatgaaat ggtatcaaaa
     1201 acaaagaata tatagcatta agtctgagga tgatagtgcg gtggcaattc gttcattaat
     1261 tttgcataaa gatgaaccta tgtatttaaa aaaacgtaca tgtgtaccta ctttgttaat
     1321 taatggggaa catgatcctt tgattaaaga taagaatcat tttaaattag aagcgcattt
     1381 tttaaatgta acgaaaaaaa tcttcgaaca ttcaggacat gcaccgcata ttgaagaacc
     1441 agaagcattt ctaagttatt atctaaattt tttaaaaagc gtatcataat atgtgatata
     1501 taaagctagg gcataaagcc cttaggcaat gtgaaaaagc tgattactat tcattatttg
     1561 atagaaatca gcttttttga aatgtatttg atatatactg ctcgttaagc ggctatcttc
     1621 cttatattaa gtgccattag tgcaaaaccg cttaacaatt aggtaaaaag agcataaaaa
     1681 aaggaagttt aatagaatgt atcatctatc aaacttcacc aaattgcgct aaacaaaatt
     1741 atagttcaat ttcgttgttt gcttcagtga ttcgtttatt tactcgactc aataatgatt
     1801 cgattttttt acgttgttgt gcattaacaa gaattaatac agttctttca tcatgctcat
     1861 tacgtttttt atcgaagtaa tcttcttgag ataaaatttt aactgcttta acaacttgtg
     1921 gttgtttgta gtttaaatga ttaataatat ctttaagata gtattctttc tctttgtttt
     1981 cgctgatgta tgtcaataca gcgaattctt caaagctaat tgaaaattcc tttttaatta
     2041 aactttttaa tttgtcagca taagtgacca ttgataacaa ctcaaagcaa tcattgattt
     2101 ttgtaattgc catggttaaa acctccctat ttgatgcatc ttgctcgata catttgcccg
     2161 ataatatatt gatatctaat ctttatttat tatagatatg ttagtcataa ttttgcatta
     2221 aataagtttt attaaatata tttaatgctc tattatttag ttaattataa ctaattaaaa
     2281 atgagaagta aacaaaaaag tgtttataaa acaaattatt cattagacac agtgattgta
     2341 tttctgggtt agcatttgga ttagtcaaaa agatcagcat tttttattat ttaccttaat
     2401 ttaatcgtca gtacaaggta atttatttaa tgaaacgatt tagcgcaatt aaatgtttca
     2461 atattcaatg tgtatctaaa gtgattcttt gtcatagctt aacgctttca agttaacttc
     2521 ttctaatatt tatacccatt tttcgaactt ttatcactaa aaaagaaaaa tttttataat
     2581 ttttttaatt tagtaattcc cagtgcagtt ttaatgataa gaaaaataca ctattttaat
     2641 gacaaaagta aaactatagc actttaaagt aaatgtatag taatccgatt gataacaagt
     2701 aatcaatatt agaaagtacg attatggttc ataatttaaa gcatcttcaa tctttaaagt
     2761 cgactcaatg aatgaattaa agttgtacat tttgacttat ttttaattaa ttgaataacc
     2821 aactatgaaa tattagcacg aataacataa attttgctat taatttggtg taaattatta
     2881 aaaaataaca ttaatttagg tcttaagaat gagttgacta tcgaatttgc taaattaaaa
     2941 tttgttgtgt caaagcatac tacccatatt agtacttatt aaaactaatt tagaccacaa
     3001 aaaaatagct tatcactttt aaaaataatt acaagttaaa atgataagct aaatgttaaa
     3061 ttaaaataat cttaaaaata taatccctgt caatatacac aacaggccta tagtttttct
     3121 tgatgtcata gctattttag gtgaaccaaa tagggcaaag tgatcaatga gtatgcccat
     3181 aagtatttga ccaaacatac caataagtgt tgttaatgct gcacctaaat ggggcattaa
     3241 aattatatta gatgttacaa aagccatgcc tagtataccg ccagtaaaat agataggttt
     3301 taatttaccg aattttaaat gacttgtttt tagttttaaa gaacgattaa aaatagcggt
     3361 taaaatcaat agcgttattg acccaattgt aaatgatact aatgatgcaa aggctggtga
     3421 atgagtatga cttgctaata aactgttaat tgtcgtttga ataggtggaa agaaaccaaa
     3481 aataaatcct aagagaagcc aaaacaataa atacttttga tcagttagta ataaattatt
     3541 cttgttaaat tgattcatta tgacgatacc gataatgagt aacaatactc caattgcttt
     3601 aattaaatta aaatcatgaa ttgtagcacc aaataatcca aatgtatcaa taatgacacc
     3661 cataataatt tgacccgcaa ctgttgcaat tacagttaat gttgcaccta attttggcaa
     3721 taacaataaa ttgccagtta aatagccaac accgagtaat ccgccaacga accaagtata
     3781 gttgaatgtt tgattactaa aaaagtgtaa tgagaaaacg tcaggattta taaaaatatt
     3841 taagacgatt aagcatatag tacctactga aaatgaaata aatgatgtat agaaaggtga
     3901 tttagtatac agtgatagtc ttgaattgac agatgtttgg ataggaataa gcattccaac
     3961 taggacacca atgatataga aaagaagcat atgaataaag tctcactttc caaagttaat
     4021 aataaaaaac atatgtatag attatcacta tagagaaaaa atacaactta tttttaaaag
     4081 agataaaagt aaagcgagtg ccataataat accccagtca atcaagaaga aagactgggg
     4141 cgttaatgat tatattcaat gaatgactac ctatgtttga gactatttct ttgcttcaaa
     4201 taaaacggtc gtcgttttat caataatttg agcagaatgt actttggtga tagcgccact
     4261 attagacttt aaaatattta aatctactaa tgcattcata ctatcacgtg caacttgctc
     4321 ggttacgatg ttgtttaatt gtggtaattg caatttgaca ggtttgtcta atgaagattt
     4381 aaaacttaat tcaagtgttt tggtcatgaa agtgtcctcc tgattaaatt gatacagatt
     4441 tgatgacttc gatgttattg tatgtttctc cagtaagtcg ctcgataagt ttgctaaatg
     4501 ttttaatttg gtcgtttgat acttcaggat taatattggc gaatcgacgt ttaaattctg
     4561 tttgtttacc atttgtatct agttttgtaa atgataatac aattgtaatg tgatttgttt
     4621 tactcatatt ttaaaacctc ctttcacact atatatcgaa agaaaaaaat aattgggtta
     4681 attttattat tcttgtttaa attctataaa aaaggttata attatatgtg acttaaatac
     4741 ttaatgttta tgattaagac acttaaagca aggagggaca tatgaacaag gtagaagtaa
     4801 ttaaatgcaa tgatgatatt aataaaatgt atgacgcgct caaagctcga tctgataggg
     4861 attatttgtt ttttaaatta gctatacata gtggtatgaa aataacagaa ttattaacaa
     4921 ttactgttga agatactaaa cgactgatag aaaaggggac actaagcgaa ttgtgtaaag
     4981 cccattatca ctcattgatt aaaattagat tgccagaaac attatctaat gagttacttc
     5041 attatattga aaacaaaagt ctgtcaaatg aagatgtatt atttcaatca ttgagaacta
     5101 atcaagtatt atctaggcaa caagcctatc gtattattca tcaagcagca attaaggctg
     5161 gcatagagaa tgtaggacta acgacattgc gtaagacatt tgcgtatcat gcatatcaaa
     5221 agggcatacc tattcctgtc attcaaaagt atttagggca tcaatcagct atagaaacat
     5281 tgaattttat cggtttagaa aatgaatgtg aacacagtat ttatatttca ttacaattat
     5341 aacgaaaaag gaggctaata atgagtttgg tttatctatt aattgctata ctagtaatta
     5401 tggcgatgat attattaaca tctaaacgta gagcaatggc taaatatgca ggatacatag
     5461 cgctgactgc acctgtaata gcatctattt attttttgtt acaagttcca tcagtaatta
     5521 aacagcatta tttgtctgtt tccattccgt ggatgacctc attagatatt aatgtagatt
     5581 tacgtttaga tggtttgagt ttaatgtttt cccttattat ttcccttatt ggaatcgctg
     5641 tattcttcta tgcaacacaa tatttatcct ctcgtaaaga taatttacca aggttttact
     5701 tatacttaac gctatttatg tttagcatgc tcggtattgt attagctgat aatacaatat
     5761 taatgtatgt gttttgggaa ctaactagcg tatcatcatt tctattaatt tcatattggt
     5821 ataacaacgg agatagtcaa tttggtgcga tgcagtcatt tatgattaca gtatttggtg
     5881 gtttggcatt attagttggt tttatcatgt tgtatatcat gacaggaacg aataacatca
     5941 cagagatatt aggacaagca gatcatatta agaaccatgc tttatttatc cccatgatta
     6001 tcatgttttt attaggtgca tttacaaagt ctgcacaatt cccatttcat atttggttac
     6061 caagggcaat ggcagcgcct acacctgtaa gtgcatattt acattcagcc actatggtta
     6121 aagctggtat ctttttatta cttagattta cacctttgtt aggtctaagc aatatgtata
     6181 tttatatcgt aacctttgtc gggttaataa ctatgttatt tggatcaatc acagctttaa
     6241 aacaatggga tttaaaagga atcctagcat actctacaat cagtcaactt gggatgatta
     6301 tggctatggt gggtataggt ggcggatatg ctcaacacca acaagacgca atagcatcta
     6361 tttatgtatt tgtgttattt gcagcattgt tccatcttat gaatcatgca attttcaagt
     6421 gtgcgctttt catgggtgta gggattttag atcatgaagc aggttcaagg gacatacgaa
     6481 ttttaagtgg catgcgtcaa ttatttccaa aaatgaatct tgttatgatg attgcggcgc
     6541 tatctatggc tggtgttcca tttttaaatg gatttttaag taaagaaatg ttcttagatg
     6601 ctttaacaca aactggacaa ttgtcccaat ttagtttgat ttcaatgatt atcatcgtat
     6661 gtatgggtgt tattgctagt atttttacat tcacatatgc actttacatg gttaaagaag
     6721 tattttggac taaatatgat tctaaagtgt ttactaaaaa aaatatccac gaaccatggt
     6781 tgtttagttt accctctctt atattaatgg tgctagtacc tgtaatcttt tttgtgccaa
     6841 atatatttgg gaaggggatt atcgttccag cattaagagg tgtatcaggt ggtaatcatc
     6901 aaattgatcc attggtacca catgtttcgc aatggcatgg atttaacata ccgcttcttt
     6961 taaccatcat cattatttta ttgggtagtg tactagcaat caaagtagat tggaaaaaag
     7021 tgttcacagg taaaattaga caaatttcag tttcaaaagg gtacgaaatg gtttaccgac
     7081 aatttgaaaa gtttactacg aagcgattta aacgtgttat gcaagatcgt ttaaatcaat
     7141 acattattat gacactgggc atatttatgg taatcatcgg atacggttat attcgtatag
     7201 gactaccaaa agttcatcag ttacatgttt ccgaatttgg accgttagaa gttatattag
     7261 caattgttac agttatcatt ggtttgtcat taatatttat acgtcaacga ctaaccatgg
     7321 tcattttaaa tggggtcatc ggttttgttg taactttatt ctttatagca atgaaagcac
     7381 ctgatttagc attgacacaa cttgtagttg aaacgattac gacaatttta tttattgtta
     7441 gcttttcaag attacccaac gtaccaaggt ctaaagttaa taaaaaacga gaaataatta
     7501 aaatttcagt atctcttatg atggcgctga ttgttgtgtc acttattttc atcgcgcaac
     7561 aagcagatgg attggcatcg atttctaatt tttatcttag agcggataaa ttaacaggtg
     7621 gcaaaaacat tgtgaatgcg atattaggag actttagagc attagataca ttgtttgaag
     7681 gtttagtatt aattattact ggattaggta tttacacatt attaaattac caagatagga
     7741 ggggacaaga tgaaagagaa tgatgtcgtt ttaagaacgg tcacgaaact cgttgtattt
     7801 attttattga ctttcggatt ctacgtcttc ttcgctggcc acaataaccc aggtggtggc
     7861 tttataggtg gtttaatatt tagttccgcg tttattttaa tgtttctagc ttttaatgtc
     7921 gaagaagtat tagaaagctt accgatagat tttagaattt taatgattat tggtgcatta
     7981 gtttcatcta ttactgcgat aatgccaaca ttttttggaa aacctttttt gtcgcaatat
     8041 gaaacaacat tgacactacc aattttaggg cacattcatg taacgacaat aacactgttt
     8101 gaattgggca ttttgttctc ggttgtcggg gttattgtaa cggtgatgct gtctcttagt
     8161 ggaggtcgat catgaattta attttattac tagttatagg atttttagta tttataggga
     8221 catatatgat attatctata aatttaattc gtattgtcat aggtatttca atttatacac
     8281 atgctggtaa ccttattatc atgagtatgg gcacttacgg ctctaataaa tcagaaccgc
     8341 tgattacagg tggaaatcaa ttgtttgtcg acccactgtt gcaagctatt gtattaactg
     8401 caatagttat aggttttggg atgactgcgt ttttacttgt cctcgtttat agaacttata
     8461 aagtaactaa agaagacgaa attgaaggct taagggggga agatgatgct aagtaatttg
     8521 ttaatattac caatgttatt accattcctt tgtgccttaa tccttgtatt tttaaaaaat
     8581 aatgaccgta tttctaaata tttatactta ggtacaatga ctatcaccac aattatttca
     8641 ttaatgctat taatttatgt tcagcggcat cgtccaatta cattggattt tggtggttgg
     8701 actgcaccat ttggaataca gtttttagga gattcgttaa gtttaatcat ggttacaacc
     8761 gcttcgtttg tgattacttt aattatggca tatggatttg ggcgtggcga acataaagca
     8821 aatcgttatc atttaccttc atttatatta tttttaagtg ttggtgtgat aggctctttt
     8881 ctaacatcag atttatttaa tttatatgtc atgtttgaaa ttatgttact tgcgtcattt
     8941 gtactcatta cacttggaca atctgtagaa caattacgtg ccgcaattat ttatgttgtc
     9001 ttgaatatta ttggttcatg gctattctta ttaggtatag gtttacttta taaaacagta
     9061 ggtacattaa acttttcaca tattgcaatg cgtttgaatg acatgggaga taatcgcact
     9121 gttacaatga tttcattaat cttcttagtc gcttttagtg cgaaggcagc gctggtcctt
     9181 tttatgtggc tacccaaagc atacgcagtg ttaaatactg agcttgcagc attatttgca
     9241 gcgttaatga ccaaagtggg cgcttatgca ttgattcgct tctttacatt attatttgac
     9301 caacataatg gtcttataca tccattgcta gtaacaatgg cagctataac tatggtcatc
     9361 ggcgctattg gtgtcattgc ttataaagat attaaaaaga ttgcagctta ccaagtcata
     9421 atctcaatag gatttatcat tttaggttta ggaacaaaca cgtttgcagg tattaatggt
     9481 gcaatatttt atttagtaaa tgatattgtt gtaaaaacat tgctattctt tattattggt
     9541 agtttagttt atattactgg ttatcgacaa tatcaatatt taaatggttt agcaaaaaaa
     9601 gaaccattct ttggtgttgc gtttattata atgatttttg ctattggcgg cgtgccccca
     9661 tttagtggct ttccggggaa agtacttatt ttccaaggtg cattgcaaaa tggcaactat
     9721 attggactag cgttaatgat tattactagt ctaattgcta tgtacagctt gtttagaata
     9781 cttttctata tgtattttgg agataaagat ggcgaagaag ttcattttaa gaaaatccca
     9841 caatatcgta aaggtatttt aagtatttta gtagttgtgg ttatcgcaat cgggattgca
     9901 gcgcctgtgc tgttaaaaat aactaatgat gcaacagaac ttaatacgaa cgatcaatta
     9961 tatcaaaagc ttgttaatcc acatttgaaa ggagaggaat aaatgaatca aattgtttta
    10021 aatattatca ttgcattctt atgggtttta tttcaagatg aagatcattt taaattctcg
    10081 actttctttt ctggatatct aattggttta attgtcattt atatattaca caggtttttc
    10141 agcgatgatt tttatgttag aaaaatttgg gtagctatta aatttttagg tgtttattta
    10201 tatcaattaa taacatctag cattagcacg attaattata ttctttttaa aacaaaagat
    10261 atgaaccctg gattactttc ttatgaaaca agactaacaa gtgactgggc aataacattt
    10321 ttaacaattt taattattat aactccaggg tctacagtaa tacgaatttc tcaagactca
    10381 aaaaagtttt ttattcatag tatcgacgtg tctgaaaaag aaaaagatag tttgttaaga
    10441 agtattaagc attatgaaga cttaatattg gaggtgtcgc gatgatacaa acaatcacac
    10501 atattatgat tattagttca ctcattattt ttggaattgc attaatcatc tgtttattta
    10561 gattaatcaa gggacctaca acagcagatc gtgtcgttac atttgataca acaagtgctg
    10621 ttgtcatgtc tattgtgggt gtcttaagtg tactaatggg caccgtttct ttcctagatt
    10681 caatcatgct cattgccatt atatcttttg taagctctgt atcgatttca agatttattg
    10741 gtggggggta tgtgtttaat ggaaataaca aaagaaatct ttagtcttat tgctgctgtg
    10801 atgttgttgt taggtagttt tattgctctt attagtgcaa taggtatcgt gaaattccaa
    10861 gatgttttct taagaagtca tgctgcaaca aaaagttcga ctttatccgt gttattaact
    10921 ttaattggtg tattaattta ttttattgtg aatacaggat ttttcagtgt gcgtttatta
    10981 ctgtcacttg tttttattaa tttaacttca ccagtcggca tgcacttagt cgctcgcgct
    11041 gcttatcgca acggcgctta tatgtatcga aaaaatgatg atcacacaca tgcatcaata
    11101 ttattaagtt caaatgaaca aaactctaca aaagcattac aattacgtgc taaaaaacga
    11161 gaagagtatc gtaagaaatg gtatcaaaac gattgaagta ttggctaaat tgttaagcat
    11221 aacaatactt ttgaagattt gttttcaaaa ttaatcatta aaagagtact ttgaaattgg
    11281 ttagtcgatt ttaaggtgct cattttttgt attataagtt aatgttgtta tgtgaaagca
    11341 agttgtttat ttatacaaac aattagtgat aaaataaaat taaataattg caaatttaag
    11401 tactgcaatc gtagacatat atctatatta aagtgctttt gtggtcgcta actcaattta
    11461 tcctctaaag tataattaca acgagatgtg atacttattt ttcaattaaa caatactttt
    11521 tagagaggtg aaagtttgga aatatttgaa acaattctta tatttatagc tgttgtgata
    11581 ctaagttcgt ttgtccatac tttcatacct aaagtacccc tagcatttat acaaattttc
    11641 ttgggcatgt tactatttat taccccaatc cctgttcaat ttaattttga ttctgaatta
    11701 tttatggtaa caatgattgc gcctttgtta tttgtagaag gtgttaatgt ttctagagtc
    11761 catttaagga aatatattaa gccagtgatg atgatggcat taggattagt cattactact
    11821 gtgataggtg tcggtttatt tattcattgg atttggccag atttacctat tggagcagca
    11881 tttgcaattg ctgccattct ttgtcctact gatgcagtag cagtgcaagc aattactaaa
    11941 ggaaaggtct tgccaaaagg agcaatgaca attcttgaag gtgagtcatt attgaatgat
    12001 gctgctggta ttatttcatt taaaatagct gttggagtat tagttacagg tgctttttca
    12061 cttgttgatg ctgttcagtt gtttttaatt gcatcaattg gtggcgcagt ggttggttta
    12121 cttataggta tggcattagt aaggttccga ttaacattga tgcgtcgagg atatgaaaac
    12181 attaatatgt ttacaattat tcaattgtta acaccatttg ttacgtattt aattgctgaa
    12241 ttgtttcacg catcaggaat cattgcagca gtagttgcag gacttgtaca tggtttcgaa
    12301 cgtgacagaa ttatgcaagt acgtacacaa ctacaaatga gttacaatca tacatggaat
    12361 atactaggtt atgttttaaa tggctttgtt ttttcaatac taggattttt agtacctgaa
    12421 gttattatta aaattatcaa aacagaaccg cacaatttaa tctttttaat aggcatcact
    12481 attgttgttg ctttagctgt ctatctattt agatttgttt gggtttatgt cttatatcct
    12541 tatttttatt tagccatcag tccattccaa aaaatgatga ctaaaaatga tgatgataat
    12601 ccaacgactg agaaaccacc aaagcgaagt ttatacgctt taattatgac gttatgtggt
    12661 gtgcatggaa caatttcttt agcaatcgca ttaacgttac cgtatttttt agcaggtcat
    12721 catgctttta cgtatagaaa tgatttatta tttattgcat ctggtatggt tattattagt
    12781 ttggtagttg cgcaagtatt attgccatta ttaacgaaac ctgcacctaa aacagtaatt
    12841 ggcaatatgt cgtttaaagt tgctagaatt tatatattag aacaagttat tgattatcta
    12901 aatcaaaaat ctactttcga aacaagtttt aaatatggta atgtgattaa agaatatcat
    12961 gataaattag catttttaaa aactgtagag aaagatgatg aaaactctaa agaattagaa
    13021 cgtctacaaa aaattgcttt taatgtagaa acaaagactt tagagtcttt agtagatgaa
    13081 ggacaaataa cgaatagtgt acttgaaaac tatatgcgtt atgctgaaag aacacaagta
    13141 tatagacaag catcattaat aagaagaatg attgtattat tacgaggtgc tttattaaaa
    13201 cgaagagtac aaacgagagt gaactcggca tcttcactta gtgttacgga taacttaatg
    13261 gaattaaata aaattaataa attagtccat tataatgtgg ttagtcgttt gtctaaggaa
    13321 acaacaaaag ataatacact tgaagttgga atggtttgtg acggttattt aatgcgaatt
    13381 gaaaacttaa caccatctaa tttcttcaac tcagcaagtg aagatacgat tactaaaatt
    13441 aaattaaatg cattgagaga acaacgtcgc attttacgtg agttgattga tacagatgaa
    13501 gtatcagaag gtacagcgtt aaaactaaga gaagccatca attatgatga aatggttatt
    13561 gtagatagta tgacgtagtt cgtaattatg ctaaaaggga ttgatgaaaa actgaatggc
    13621 ttttcatcaa tcccttttat ttaggggaat tgaatagata gttttaattt ttctctgtat
    13681 gaacagctgt ttttaataat atagatgaaa ccattgctaa tatcatagca acaacaattg
    13741 ataagttgaa ctgattatcg atagaaaata atataccacc aattaatgat cctaatccga
    13801 ttcctaaatt taatattgaa atatcatagc tcaatatttg acttggatcg ccttttaaaa
    13861 atttaagtaa agcatattga actgttggat ttgttcccca atgaaataaa ttccatgtaa
    13921 atatagataa aattaaaagt atgacatttt gtccagtcaa taagatgctc ataatagaaa
    13981 taatatagac aattataatg atatttccag caatcctata gcctttcttt tcagttaaat
    14041 tatttcctaa ttttgaacct aaaacacctg caatacctga gaaaaataaa cacaaactta
    14101 ctatccagat gctatgacct ttacttttta ctaaaggctc tatatatgta aataacatgc
    14161 tattagatat catcatgatt aataaaaagc ccaacatgat taatgtgcct ctgaaattta
    14221 ctaattggta tttagaatga ttattaatac tataatgtga tttattcggg ataaataaca
    14281 taatacctag tagacataaa aaagtgataa tcgcaatgat ataaaacgga atcttccaat
    14341 tgtaatggga agcaattaat gtacctatgg gtataccaag tatatttgct gcactaaagc
    14401 ctacgtatat gttagcgata actgatgctt tgtattctac tggaacaata tttgctccag
    14461 tagacattaa ttttacagtg attaatgaag cgatgcccgc cataaagatt ctaacaatac
    14521 atagaaagaa ataattggtc attacagttg taagcatgct gaaaataata aaaagtatta
    14581 aacaggatac taataatttt ttgtcattaa tttgactagt tacctttgtt aagagagggc
    14641 ttagaattga aaaagcaaca gcatacaatg ttaccaactg acctattaaa gcattactta
    14701 catgaaattc tttactcatt tcatttaaca ttcctacaac aattaagtct gaaacaccaa
    14761 caataaacat tgtgaaaaag ccaatcaaag ttacaatttt aatatttgac atatcttact
    14821 cctcaaaatt aagttcttta acattattaa ttataaaatt tggattttca gtgtttaata
    14881 cttcaatact atgacttccc caagttacag cacaagaatc aatattggca tttttagcca
    14941 ttttaatatc aaaaacagca tctccaacat atatagtgtc tttgttatgt aagtcatatt
    15001 tttgtaatat atatataatt ccatcaggag aaggtttata attcgttact ttatacttca
    15061 acaaatagat tatcaacacc taatttagaa agatttcttt ctaatacatt tgttttttta
    15121 ctagaaacaa cgaataactt atggttttga tgaagtgttt tgagctgttc ttgcattccg
    15181 tcatatattt ttaaatgttt tgcttcagtt tctttataat tttctctaaa caatgtcatt
    15241 agttcggtga gttctttgtc atttagctta aaatcactca ttaaattaaa agatttttca
    15301 atcggaatac ccatataata ttctatttgt tcatcgctag gtatattcaa acctcttttt
    15361 tgtggccatg tacaccaaaa acacagggaa aaccttgaga ttgaattact tcttgagaag
    15421 tgttgatttc agcttctaac tatgcatgtg agttcttaga attttttatt tcactttgca
    15481 aaagcaacat tttatcacct caaaatagta tcgattaatt tatattatat ataaattaat
    15541 taaatattgc aaattaattt tatgtaaact taattaaagg gatttgaagg tgttgttaac
    15601 atttgagatt taattgaaat aagttttaaa aattggagga gatagattaa tcgaagtcat
    15661 ttaaagatga agataagtgc attcacaaaa aatagccaca ctcatatgac atcggatgag
    15721 tgtggcttaa ggatctatgg ggggaggaaa ccatagatgt ttactttgat aggccagatt
    15781 aaatatcaaa gtatgcgatt atttatagct tgatgcaaaa gtggtatgcc tattaaaagt
    15841 tactgcacat agcttttaat attccgttca aaggaaaggg gcatacaatt gaacaatctg
    15901 taatagtact tttaaccagc tatgctaaaa gtctagtagg gagaacagtt gtccaatcgc
    15961 ataagaacct ctaacttcgt tagtacgatt aagaaaagct ttttagttag tatgtaatac
    16021 aatttattga cgcgcgtgaa tctcttttat aagagtgtgt agggaatggc gttgtataaa
    16081 ttgtattaga agaacttcta acgcatctct gtggttaaaa gagatgaagg gaacgacagt
    16141 ttaattaaaa acacataaag cttttaactt tactctttga tttaaagagt gacaaatgtt
    16201 tatagtttaa ttaaaactgc ataagaactt ctagcttttc tctttcgctc aaagagaagt
    16261 tctaatacca ccatatcgtg cgatcgggaa cggtatatat atattaatag gagggtaata
    16321 tatatttaac gcacgatatg ggactattag ccttcgactt tgttatgttg atgtgtggcc
    16381 taaaatattg gagataccaa tattttaggt tgcatcaaca tcaattcatc ttacttcatt
    16441 aaaacacagc gtgttatttc atgcttccgt gtacagtttc aatatttgat ttcatcattt
    16501 tgtagtaaga gtcaccttta gtgccttctt taccgattga atctgtgtac acttcaccaa
    16561 agatatcttt cttcgtttct tcagataaac tttccattgc tttcttatca acacttgttt
    16621 ctactaataa gtgttttaat ttgtgctttt taacaaactc aatagcttgt ctcatttgtt
    16681 ctggtgtacc ttgtttttca gtgttaattt cccaaatata acctggtgta ataccgtatt
    16741 gttttgagaa gtacttgaag gcaccttcac ttgtaatcat ggcacgttgt tcttttggaa
    16801 tgtcattaaa tttgtcttta ctgtcgttat ttaatttttc caattgagca atgtatttgt
    16861 taccttgctt ttcataatct gctttatgtt ttttgtcgtt atcgataaat gtttgttgaa
    16921 ttgtttttac gtatttaata ccgttatcta aacttaacca tgcgtgtgga tcttgtttat
    16981 ctttgttgcc ttcttcaccg tttaaataga taggtttaac atcttttgat actgcgataa
    17041 cttttttatc ttttaatgat ttaccagcct gttctaaggc tttttcaaac caaccgttac
    17101 cagtctctaa atttaatccg ttgtataaaa taacgtcagc gtcagttaac tttttaatat
    17161 ctttaggttt aacttcatat tcatgaggat cttgaccaac aggtacaata ctatgaatat
    17221 cgacgttgtc tccaccaaca tttttagcca tatcatataa aattgaattc gtcgttacta
    17281 cttttagttt gccatttgac ttatcactgc tttgtttacc accagtacca catgcagcaa
    17341 ctaaaagtaa taaggctaat aataaaggta ctaatttttt catgttaaac ttcctcgttt
    17401 ctttctattc gtaaattttg tgaaaaataa tgtgatgata taaattacaa acgtacaaag
    17461 tacgattgtt gcaccactag gaatgttgta aatatagctg taataaagtc cgacaattga
    17521 acttatgaca cttattaaac ttgctataat catcattgag tatagttttt tactaattaa
    17581 aaatgctgta gatgcaggtg taattaataa tgcaactaca agaataatac ctaccgtttg
    17641 aatacttgct actgttacta atgagagtaa caacatcaca aagtaatgta ataacgtcgt
    17701 atttagacca ctcattctac taaaagttgg atcgaatgta gaaatcatta atggacgata
    17761 gaaaataatg attagaataa ggacgattga accaatcact atagttgtta aaaatgcact
    17821 atttgtgatt gccagtaaat taccaaacag aatatggtac aaatctgtcg tagtgtttat
    17881 taagctaata ataataatcc ccgaagctaa gaaagcggta aaactaattc caatagcggc
    17941 gtcaggtttc gttttactac tagatgtgat ataaccgata aaaatacttg cgatcatacc
    18001 agttataagt gcgcctacaa acattggaat accaaataag aatgataggg caacaccagg
    18061 taatactgcg tgactcattg catctcccat taatgaaaga ccacgtaata caattaaact
    18121 accaactgta ccacaaacta tccctacaat aattgaagtt atcaatgctc gattcaagaa
    18181 ttgatatgta aataaatgtt cgacaaactc taacatgtta tattgctcct ttgactaggg
    18241 tcactacagt cagtgctact cataaatgtt tcgtttaagc gagtgacact catagcctct
    18301 tcactatcac caaagtatcg taatgtttga tttaatagaa taatgcgatc aaagtattgc
    18361 tttgcttttg ataaatcatg gtggatgata agaataagtt ttccttgttg ttttaagttc
    18421 tcgatttttg tcatgattaa tttttcacta ctaaaatcaa ttccgacaaa cggctcatct
    18481 agaaaataaa cttcactttc ggacatcaat gctctcgcta ctagcacacg ttgtaattgt
    18541 ccaccactta attctgaaat ttgtcgatga cgtaaagatt ctaattctaa atcgcttaat
    18601 aactgtttga gtttatccct tgctgattta ttaggtcgtc taaaccatcc aatttctttg
    18661 tagcaacctg ataaaatcac ttgttccaca cttataggaa aatctaaatc aatatgtgct
    18721 ttttgtggaa tatatgtaat atgttgcagt tgttgttgta taggtttgtt atgtaacaat
    18781 ttagtaccgg tagcattaaa ttcaccaatt aaagacttga taagggaaga tttaccagca
    18841 ccattcgggc ccatgatacc aattatttcg ccgcgtactg gtatcgataa ggaaatgttt
    18901 ttaagtacat gcttattacc taaaaacaga tttaaatctt ttgtttctaa caaacgttta
    18961 tacctcctaa ttaaaagttt aggctaacct aattaattgt ataataaact gagaatattt
    19021 atcatgtcaa gtaaattcgt gatataatat agacaatgta tgtgaggtga aagtatgtta
    19081 actgaagaaa aagaggacta tttaaaggca atccttacga ataatggcga taaaaatttt
    19141 gtgacaaata aaatcttatc tcaattttta aatattaagc ctccatctgt aagtgaaatg
    19201 gtaggacgtc tcgaaaaagc aggctatgtt gaaacaaaac cgtacaaagg tgttagatta
    19261 acagaggatg gtttaacgca tacgcttgat atcattaaga gacatcgact attagaatta
    19321 tttttaatag aaatattgaa atataattgg gaagaagtac atcaagaagc agaaatttta
    19381 gaacatcgaa tttcagattt atttgttgaa aggctggata gcttgttaaa tttcccagaa
    19441 acttgcccgc acggcggtgt gattcctaga aataatgaat ataaagagaa atatataaca
    19501 acgattttga attatgaacc tggtgatatc gttacaatca aacgtgtgag agataagact
    19561 gatttgctaa tatatttgtc tagtaaagat atctctattg gtaatgaagt ggaaattgta
    19621 tcgaaagatg aaatgaataa agtaattatc atcaaacgta atgataatgt aattattgtc
    19681 agttacgaaa atgcaatgaa catgtttgct gaaaaataaa ataaagaagc cataaagata
    19741 tccatgattg tactgataaa gacatatgga taattgcttt aggcttcttt tttattagtt
    19801 aatttatcaa gtgagtatat ttgagtaaaa tattcactgc ataaagattg aagataatcc
    19861 agattgtact ataaatgaag ataggtacat gactgagttc tttaagtgca ctaccatccc
    19921 actgtggact cggacgctgg aaagtcaatt tagcaatcgt ccaactagat tgtagaactt
    19981 cgcctaataa tacacctaaa atatattgat aactcattgt gacaagtagt tgaatttcta
    20041 ctatattttc atcttttaat ataaaataca acatgataga aattaaagtt ataacaacaa
    20101 tgggtgagcc ttttctagat gttaaaatta aaaaataaat aaatatcaat aaataggtaa
    20161 atataaagaa actaggtatc tgataatggc tcgacgctag accaatcaat aacataatag
    20221 gtggcataaa ataaccacca atcgttgtaa gccattggcc tgctagatgt ctagattgtg
    20281 taattgcgaa tccttgttgt aatgtctgtt gtcgctctcg tgggcttgtt acaatgacta
    20341 aatcttttgc acggccacca gcgagtttat taaacagtac atgaccaaat tcatgtgtta
    20401 aaacagggat atagtttaaa atgacatcta aatagttcaa aacaggctta tgtctatatt
    20461 gatgaatagc aatataacaa gctgcaacaa taacgataat gtatatatta agttgaattg
    20521 tcgtattaaa aaagttggat aaataattca ttgttaacct catataagat attaatttaa
    20581 agtttgctta tcacttatta taaatgatat tggcatcaat agcgttagac tttagactta
    20641 ccttagttaa actaatttta atttttgaaa aggtgaatat gtgttaaaat aaagcaaaat
    20701 catttcgata taaataggat gaatataaat actgttaata ttgattacac taacataata
    20761 atgaaataag ataggagatt cctgttatga ctgttgaaga aagatccaat acagccaaag
    20821 ttgacatttt aggggtcgat tttgataata caacaatgtt gcaaatggtt gaaaatatta
    20881 aaaccttttt tgcaaatcaa tcaacgaata atctttttat agtaacagcc aaccctgaaa
    20941 tagtgaatta cgcgacgaca catcaagcgt atttagagtt aataaatcaa gcgagctata
    21001 ttgttgctga tgggacagga gtagtcaaag cttcgcatcg tttaaagcaa cctctagcgc
    21061 atcgtatacc tggtattgag ttgatggatg aatgtttgaa aattgctcat gtaaatcatc
    21121 aaaaagtatt tttgctaggg gcaactaatg aagttggaga agcggcacaa tatgcattgc
    21181 aacaaagata tccaaacata tcgtttgcac atcatcatgg ttatattgat ttagaagatg
    21241 agacagtagt gaaacgaatt gaactgttta aacctgatta catatttgta ggtatgggat
    21301 tccctaaaca agaagaatgg attatgacac atgaaaatca atttgaatct aaagtgatga
    21361 tgggcgtagg tggttctctt gaagtatttg ctggggctaa aaagagagcg ccttatatct
    21421 ttagaaaatt aaacattgaa tggatatata gagcattaat agattggaaa cgtattggta
    21481 gattaaagag tattcctata tttatgtata aaatagccaa agcaaaaaga aaaataaaaa
    21541 aggcaaaata gtcatgatga caaaataaaa ccgaggaaat ccttaaatgg agattctcgg
    21601 ttttttcggt ttatttaata acgaagcggg actcatcgag tttgtttcta aattcttttt
    21661 gttcggcttt ggatttcttt ttaaaatcgt ttaggaaagc ttcatattta ggtaatacat
    21721 catcaagttc accgtaatct tttaactttc cgccttcaat ccaagcaatc ttagtacaaa
    21781 attgtctcac ttggcctaag ttatgactaa cgaaaaagat ggttttgttt tgctctttaa
    21841 actcgtaaat attatctaaa catttttgtg caaaagtttg gtcacctaca gataaagctt
    21901 cgtcaatgac taagatatct ggattaactg tgatattaat tgaaaaacca agttttgcac
    21961 gcataccact tgaatacttt ttaactggtt gatagataaa ctcaccaagt tcactaaatt
    22021 caataatctt aggtgtcatc gctttaattt ctttacgctt aaagcccata cataacattt
    22081 taaattcgat attttcaatc cctgtaagtt gtccactcaa gccagcacta attgcgataa
    22141 cgctgacttc accattacga tccacttttc caacagtagg cgacaatgaa cccccaatga
    22201 tattgctcaa cgttgatttg ccggaaccat tgatgccaac aagccctatg acgtcacctt
    22261 catatgcttt taaactaatg tcatctaaag cgaaaaatgt tttgttttta tgtttgggaa
    22321 tgagcgcatc tttcatacgt tctttatttg tacgataaat acgatattct tttgttacat
    22381 ttttaatgtt taccgaaacg ttcatttgta gaccttcctt attcacattt atctagatta
    22441 taatatacta ctcaacagtt gttaaatttt aaaacctgtt gtaaagtgta tagaagattt
    22501 tgttattatc agagttggtg ttttgacaca aaatgttaat catcaatgat aacaatgata
    22561 tttaaaaact aaacttattt caacttacat gattgtatac tataatgtat ttgtaataaa
    22621 ctaatatttt aaagaactag acaa
//
LOCUS       DAKSQE010000034        22522 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.034, whole genome shotgun sequence.
ACCESSION   DAKSQE010000034 DAKSQE010000000
VERSION     DAKSQE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22522
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.034"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            108..1274
                     /gene="norA"
                     /locus_tag="O0V33_001147"
     CDS             108..1274
                     /gene="norA"
                     /locus_tag="O0V33_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HCX8975793.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADVSPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSVVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPAILTLVLAFGL
                     SAFETLYSLYTSYKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     IYSVIVLVLLVIADGYWTIMVISFVVFIGFDMIRPAITNYFSNIAGDRQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKQQDL"
     gene            1571..2023
                     /locus_tag="O0V33_001148"
     CDS             1571..2023
                     /locus_tag="O0V33_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975794.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENVKKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
     gene            2197..2679
                     /gene="ybaK"
                     /locus_tag="O0V33_001149"
     CDS             2197..2679
                     /gene="ybaK"
                     /locus_tag="O0V33_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCX8975795.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGQEVAELIQAN
                     VKTVFKTLVLENAKHEHFVFVIPVSETLDMKKAAAMVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            2929..3693
                     /locus_tag="O0V33_001150"
     CDS             2929..3693
                     /locus_tag="O0V33_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCX8975796.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYKQKYHFIGGTL"
     gene            3690..4610
                     /gene="pfkB"
                     /locus_tag="O0V33_001151"
     CDS             3690..4610
                     /gene="pfkB"
                     /locus_tag="O0V33_001151"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCX8975797.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDADVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLSIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTICVLDGE"
     gene            4616..6574
                     /locus_tag="O0V33_001152"
     CDS             4616..6574
                     /locus_tag="O0V33_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCX8975798.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDIASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKVGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDHADDEATKEEEAE
                     AEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIKNATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GANSFDPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFASGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKVMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YVFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALVVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            6877..8058
                     /gene="nagA"
                     /locus_tag="O0V33_001153"
     CDS             6877..8058
                     /gene="nagA"
                     /locus_tag="O0V33_001153"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCX8975799.1"
                     /translation="MSELIIYNGKVYTEDDKIDNGYIHVKDGQIVAIGEVDDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDKALINIAKYEVEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANGLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAAE
                     RGAKHVTHLYNAATSFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLTNGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
     gene            8279..9628
                     /locus_tag="O0V33_001154"
     CDS             8279..9628
                     /locus_tag="O0V33_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCX8975800.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLDTIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVMLNYEFNEPRPTIGQSDEDEKSE"
     gene            9842..10681
                     /locus_tag="O0V33_001155"
     CDS             9842..10681
                     /locus_tag="O0V33_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCX8975801.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIG
                     DLK"
     gene            10813..11796
                     /gene="csbB"
                     /locus_tag="O0V33_001156"
     CDS             10813..11796
                     /gene="csbB"
                     /locus_tag="O0V33_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HCX8975802.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVARQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSIENDEKDTHKVYSK"
     gene            complement(11868..12923)
                     /gene="saeS"
                     /locus_tag="O0V33_001157"
     CDS             complement(11868..12923)
                     /gene="saeS"
                     /locus_tag="O0V33_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HCX8975803.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCSEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENKDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITT"
     gene            complement(12923..13609)
                     /locus_tag="O0V33_001158"
     CDS             complement(12923..13609)
                     /locus_tag="O0V33_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCX8975804.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            complement(13584..14057)
                     /locus_tag="O0V33_001159"
     CDS             complement(13584..14057)
                     /locus_tag="O0V33_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCX8975805.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            complement(14399..14839)
                     /locus_tag="O0V33_001160"
     CDS             complement(14399..14839)
                     /locus_tag="O0V33_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HCX8975806.1"
                     /translation="MNTKYFLAAGAIITTLALGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            complement(15119..15703)
                     /locus_tag="O0V33_001161"
     CDS             complement(15119..15703)
                     /locus_tag="O0V33_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975807.1"
                     /translation="MIVYILINIAIVVLITGFNLYRHQMQHLSLSAMLLSITINAFIN
                     TFIIDKYNFITLCTITMFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVT
                     DFSSTQSIYMSIPFLAPAIFIIGGIMLFISTFNSLDESAENNNKIKKLMIKGLIIINI
                     SFIVMMVLTPYWYLYLIVYLIFLLFLLWQKVYKF"
     gene            complement(15802..16515)
                     /gene="queE"
                     /locus_tag="O0V33_001162"
     CDS             complement(15802..16515)
                     /gene="queE"
                     /locus_tag="O0V33_001162"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCX8975808.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVIAQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
     gene            complement(16519..16938)
                     /gene="queD"
                     /locus_tag="O0V33_001163"
     CDS             complement(16519..16938)
                     /gene="queD"
                     /locus_tag="O0V33_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCX8975809.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            complement(16940..17608)
                     /gene="queC"
                     /locus_tag="O0V33_001164"
     CDS             complement(16940..17608)
                     /gene="queC"
                     /locus_tag="O0V33_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCX8975810.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            17960..18553
                     /locus_tag="O0V33_001165"
     CDS             17960..18553
                     /locus_tag="O0V33_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCX8975811.1"
                     /translation="MILVIDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDIINMK
                     PKAIVISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVH
                     GHTTQLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAFEHIT
                     FPVFGVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
     gene            18537..19688
                     /locus_tag="O0V33_001166"
     CDS             18537..19688
                     /locus_tag="O0V33_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HCX8975812.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHVMCTHSLAKDDIYAVAYSFENAESINSTYEHQT
                     TYVSKHHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEVYKTVFQMTSMVS
                     GTLKTNTDLTQILTSLFPCGSITGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            19688..20296
                     /locus_tag="O0V33_001167"
     CDS             19688..20296
                     /locus_tag="O0V33_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HCX8975813.1"
                     /translation="MQLFETMKIDNGHIPRITYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHNGQYRLKIVLHAESEFETIVSPLPEKSSFTAKFQVLPKAVNPTFINNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRIFLINSLREVADVHLCL"
     gene            20362..20568
                     /locus_tag="O0V33_001168"
     CDS             20362..20568
                     /locus_tag="O0V33_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975814.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISF
                     ILVYALIHELMPFVVRAMDLLYHQ"
     gene            20656..21366
                     /locus_tag="O0V33_001169"
     CDS             20656..21366
                     /locus_tag="O0V33_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HCX8975815.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGYNIKFR
                     PINENELEVGAFKDVNHN"
     gene            21350..22354
                     /locus_tag="O0V33_001170"
     CDS             21350..22354
                     /locus_tag="O0V33_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCX8975816.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEQMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
BASE COUNT         7829 a         3171 c         4084 g         7438 t
ORIGIN      
        1 aatacaatat agaaactttt tacgaatatt tagcatgaat tgcaatttgt cgtggaaaag
       61 aagaataaca gatttaagaa tgacatggag aaaaaagagg tgagcatatg aataaacaga
      121 tttttgtctt atattttaat attttcttga tttttttagg tatcggttta gtaataccag
      181 tcttgcctgt ttatttaaaa gatttgggat taactggtag tgatttaggg ttacttgttg
      241 ctgcttttgc gttatctcaa atgattatat cgccgtttgg tggtacgcta gctgacaaat
      301 tagggaagaa attaattata tgtatagggt taattttatt ctcagtgtca gaatttatgt
      361 ttgcagttgg ccacaatttt tcggtattga tgttatcgag agtgattggt ggtatgagtg
      421 ctggtatggt aatgccgggt gtgacaggtt tgattgcaga tgtttcacca agccatcaaa
      481 aagcaaaaaa ctttggctac atgtcagcga ttatcaattc tggattcatt ttaggaccag
      541 ggattggtgg atttatggca gaagtttcac atcgtatgcc attttatttt gcaggtgcat
      601 taggcatttt agctttcata atgtcagttg tattgattca tgatccgaaa aagtctacga
      661 caagcggctt ccaaaaactt gagccccaat tattaacaaa aattaattgg aaagtcttta
      721 ttacgccagc aattttaacg ctcgtcttag cgttcggttt atcggcattt gaaacactgt
      781 attctttata tacatcgtat aaagtaaatt attcacctaa agatatttcg attgcgatta
      841 cgggtggcgg tatctttggt gcacttttcc aaatttattt ctttgataaa tttatgaaat
      901 acttctctga gttaacattt attgcatggt cattaatata ttcagttatt gtattagtgc
      961 tattagttat tgccgatggt tactggacaa ttatggtaat tagctttgtt gtctttatcg
     1021 ggttcgatat gataagacct gctattacaa attatttctc aaatattgct ggtgatagac
     1081 agggatttgc aggtgggtta aactcgacat tcactagtat gggtaatttc ataggtcctt
     1141 taatcgcagg tgcgttattt gatgtacaca ttgaagcacc aatttatatg gctataggtg
     1201 tgtcattagc aggtgttgtt attgttttaa ttgaaaagca acatagagct aagttaaaac
     1261 aacaagattt gtaatatcgc acatggttgt cattcgatta atgtatttcg acaactaaag
     1321 catttcaaaa taagcatcga ttttcttaca attatagtga agaaaagtcg atgctttaaa
     1381 tttataaaga ttaaaaactt ttaaatgttt tagtctttat atttaaatgt tatatgtaac
     1441 aaaaaatgat tttgagtaat aaacatgtta caaatattac attcttttta aattgcaatc
     1501 cacataccta attcattaac gttaatgtgt taagatgata aaaaatgagt aaggaaatgt
     1561 gggtaagggg atgacagtaa aaaatttatt tttaggcttt gttgctgtaa tattaaccgt
     1621 ttgtttaatt ggtttattaa tattagcaac aaatgaagat gcgcttgcta aggtacataa
     1681 aacgattaat acgcttaacg cgataaatgt atcaactgaa gatacttata aaaagaaaat
     1741 ggatattctc aatattcata ctgctaaagc atctgaagtg aatgaaaatg tgaaaaagca
     1801 aaatcatttt aagcatcgtg tgaatgcaaa taaatcaaat tcttttaacg aacaagagtg
     1861 ccaagttatt gctgatcgtt atgcagataa gcatatcaat gataattatg gtttagaaag
     1921 aatttctaag acaaatcatg gatataatta tgtttattcc aatgataatt caacgagtaa
     1981 gcaacatgta agtatttcaa atcaaggcat aataacgaaa taatagatgg aacagtgtat
     2041 tctaattgga tatactgttt ttattttgca atattttaat ttaaaaaggt gaaatcaact
     2101 tataaaatga tgtaaatgtt atgtcaaaat caaccaatcc gtaatgtatt ttaaaatgtt
     2161 aatatagttc tgaagaagta taaatgaggt gttgaaatgg ctaaaaataa gaaaactaac
     2221 gcgatgcgta tgcttgatcg tgcaaaaatt aaatacgaag ttcatagctt tgaggtacca
     2281 gaagaacatt tatctggcca agaagtcgca gaactcatac aagcaaatgt taaaacagta
     2341 tttaaaacac ttgttctaga aaatgcaaaa catgaacatt ttgtatttgt tatcccagta
     2401 agtgaaactt tagatatgaa aaaggcagct gctatggttg gagagaagaa attgcaactt
     2461 atgcctttag ataatttaaa aaatgtaacg ggatacattc gtggtgggtg ttcacctgtt
     2521 ggtatgaaaa cattgtttcc aacagtcgtt gacaaatcct gtgaaaatta tagtcatatc
     2581 agtgtgagtg gtgggcttcg aacaatgcaa atcacgatag ctgttgagga tttgattaca
     2641 ataactaaag ggaaaattgg agcagttatc catgaatgat taataacaac aaaaagtatg
     2701 gggcaagatt aggagtgcga cagagatgaa atttttataa aattcatttc tgctacactc
     2761 ctttttgatt gaaatagcat tttacgatca taaacagtca tgataattga gtatttgaac
     2821 ataaaaatgt aattttatcg tcacaatttg attgtttgtg attgtttttg gtaatttatg
     2881 attgaaaagt gaaagcgtac tcattataat acagagtgag atggggtgat gatgataatt
     2941 acagaaaaaa gacacgagtt aatattagaa gaactttcgc acaaagattt tttgacttta
     3001 caagaattaa tagatcgaac tggctgcagt gcttcaacaa tacgaagaga tttatctaaa
     3061 ctacaacaat tagggaaatt acaacgtgtg catggtggtg caatgttaaa agaaaatcgt
     3121 atggttgagg cgaatttaac tgaaaaatta gcaactaatc tcgatgaaaa gaaaatgatt
     3181 gctaaaatag cagctaatca aatcaacgat aatgaatgct tatttatcga tgctggttca
     3241 tctacattgg agctaattaa atatattcaa gcgaaagata tcattgtggt aaccaatggt
     3301 ttaacacatg tagaagcttt acttaaaaaa ggtattaaaa caattatgct aggtggtcaa
     3361 gttaaagaaa atacacttgc tacgattggt tcaagtgcca tggagatatt aagacgatat
     3421 tgtttcgata aagcttttat cgggatgaat ggattagata ttgaacttgg attaactact
     3481 cccgatgagc aagaggcatt agttaaacaa acagcaatgt cattagccaa tcaatcattt
     3541 gtacttatag atcattctaa gtttaataaa gtatattttg ctcgtgtacc tttgctagaa
     3601 agtacgacaa tcatcacatc tgaaaaagca ttaaatcaag aatcgttaaa agaatacaaa
     3661 caaaagtatc actttatagg agggacttta tgatttatac agtgactttc aacccttcaa
     3721 ttgactatgt catttttacg aatgatttta aaattgatgg tttgaacaga gcaacagcaa
     3781 catataaatt cgctggggga aaaggtatta atgtctcacg tgttttaaag acattggatg
     3841 ttgagtcaac tgctttggga tttgcaggtg gatttcctgg gaaattcatt atagatacat
     3901 taaataacag tgcaattcaa tcgaatttta ttgaagttga tgaagataca cgtattaatg
     3961 tgaaattaaa aacaggacaa gaaacagaaa tcaatgcacc gggtcctcat ataacgtcaa
     4021 cacaatttga acaactgttg caacaaatta aaaatacaac aagcgaagat atagttattg
     4081 ttgctggaag tgtaccaagt agtattccaa gcgatgcgta tgcacaaatt gcacaaatta
     4141 cagcacagac aggtgctaaa ttagtagtcg acgctgaaaa agaattagct gaaagcgttt
     4201 taccatatca tccactattt attaaaccta ataaagatga attagaagtg atgtttaata
     4261 caacagtgaa ctcagacgca gatgttatta aatatggtcg tttgttagtt gataaaggtg
     4321 cgcaatctgt tattgtctca cttggcggtg atggtgctat ttatattgat aaagaaatca
     4381 gtattaaagc agttaatcca caagggaaag tggttaatac agttggctct ggtgatagta
     4441 cagttgcagg catggtggct ggaattgctt caggtttatc gattgaaaaa gcatttcaac
     4501 aagcagtcgc atgcggtact gccacggcat ttgatgagga cttagcaaca cgggatgcta
     4561 tagaaaaaat aaaatcacaa gttacgattt gcgtacttga tggggagtga aaataatgag
     4621 agtaacagag ttattaacaa aagatacgat agcaatggat ttaatggcaa atgacaaaaa
     4681 tggtgttatt gatgagttag taaatcaatt agacaaagca ggtaaattaa gtgatatcgc
     4741 gtcatttaag gaagcgattc acaatcgaga atcacaaagt acaactggta tcggtgaagg
     4801 tattgctatt ccacatgcca aagtggccgc agttaagtca ccagctattg catttggtaa
     4861 atctaaagta ggcgtagatt atcaaagttt ggatatgcaa ccagcacact tattctttat
     4921 gattgcagcg ccagaaggtg gcgcacaaac acatctagat gctttagcta agttgtctgg
     4981 tattttaatg gatgaaaatg tacgtgaaaa attattacat gcatcgtctc cagaagaagt
     5041 gttagcaatt attgatcatg ctgatgatga agcgacaaaa gaagaagagg cagaagctga
     5101 agcacaacaa gttgcaactg cagaacaatc atctaaacaa tctaacgagc catatgtatt
     5161 agctgtaact gcttgtccaa caggtattgc acacacatat atggcacgtg atgcattgaa
     5221 aaagcaagcg gataaaatgg gcattaaaat taaagttgaa acgaacggtt caagtggtat
     5281 taaaaaccat ttaacggaac aagatattaa aaatgcaaca ggtatcattg ttgctgctga
     5341 tgttcatgtt gagacggatc gcttcgatgg taaaaatgtc gtagaagtac cagtagcaga
     5401 tggtattaaa cgcccagaag aattaattaa taaagcatta gatacaagtc gtaaaccttt
     5461 tgttgcccgt gatggtcaaa gaaaaggtaa ctcaaatgac agtcaagaaa aattaagccc
     5521 aggtaaagct ttctataaac acttaatgaa cggtgtttct aacatgttgc cacttgtaat
     5581 atctggtggt attttaatgg caatcgtgtt tttatttggt gcaaactcat ttgatccaaa
     5641 aagctcagag tacaatgcgt ttgcagagca gctttggaac attggtagta aaagtgcatt
     5701 cgcgttaatc attccaattt tatctggatt cattgcacgt agtattgcgg ataaacctgg
     5761 attcgcttca ggtcttgtag gtggtatgtt agcaatttca ggtggttcag gatttattgg
     5821 tggtattatt gcaggtttct tagcaggtta cttaacacaa ggtgttaaag tgatgacacg
     5881 taagttacca caagcattag aaggattaaa accaacatta atttacccat tattaacagt
     5941 gacagctaca ggcttattga tgatttatgt ctttaatcca ccagcatctt ggttaaatca
     6001 tttattatta gatggattaa ataatttatc aggttctaat attgtattat taggtttagt
     6061 cattggcgct atgatggcga ttgatatggg cggtccattc aacaaagcgg catatgtttt
     6121 tgcaacaggt gcgttgattg aaggtaatgc agcaccaatt acagctgcaa tgattggtgg
     6181 tatgattcca ccgttagcaa ttgcgacagc gatgttaatt ttcagacgta aatttacaaa
     6241 agaacaacgt ggttcaatta tccctaacta tgtgatgggt atgtcgttta ttacagaagg
     6301 tgcgatccca ttcgcggctg ccgatccatt acgtgttatc ccttcaatga tgattgggtc
     6361 aggtataggt ggcgcaatcg ctttaggttt aggttctcga attactgcgc cacacggtgg
     6421 tattatcgta attgttggta cggatggtgc acatttacta caaacactta ttgcacttgt
     6481 agtaggtaca ttagtttcag cattaattta tggacttatt aaaccaaagt taactgaaac
     6541 agaaatcgaa gcttcaaaat caatggacga ataactttta tgatttaaaa tgattgttag
     6601 aaatgacgtt acatcaattt gtcagtcgat tgaatgtatg ttaatttaat atatcgtgtt
     6661 aacggtagtt tatataaaac tattaaaaca ccttccatta attcagtttt tatgaatgga
     6721 tgcgaaggtg tttttaattt ttagtatgtg aatgtagtca ttacaaaaat aaatatagtt
     6781 tttaaagttt ttgcaaaaag ttatgccaaa cgaagcagat atagtaaaat atgagtgtct
     6841 taaagtgaaa atttataaat aaagaagggt ttatacgtgt cagaattaat tatatataac
     6901 ggcaaagttt atactgaaga tgacaaaatc gataatggtt acattcatgt gaaagatgga
     6961 cagattgttg caattggaga agtggatgat aaagcagcga ttgataatga tacgacaaat
     7021 aaaattcaag tgattgatgc aaaaggtcat catgtattac caggttttat cgatatacat
     7081 attcatggtg gttatggaca ggatgcaatg gatgggtcat acgatggctt aaaatatcta
     7141 tccgaaaatt tgttgtctga agggacgaca tcgtacttgg ccactacaat gacgcaatcg
     7201 actgataaaa tagataaagc acttataaat attgctaaat atgaagtgga gcaagatgtt
     7261 cacaatgcag cggaaattgt aggtatacat ttggaagggc catttatatc tgaaaataaa
     7321 gttggtgctc aacatccgca atacgttgta cgcccattta tcgataaaat taaacatttt
     7381 caagagactg ctaacggact aataaagatt atgacgtttg cacctgaagt tgaaggtgca
     7441 aaagaagcgc ttgaaacgta taaagatgac attatttttt caattggtca tacagtggca
     7501 acatacgaag aagcagttga agctgctgag cgaggagcta aacatgtcac gcatttatat
     7561 aatgcagcga cgtcattcca acatagagaa ccaggtgttt ttggagcagc atggttgaat
     7621 gatgctttac ataccgaaat gattgttgat ggcacacatt ctcatccggc atcggttgca
     7681 attgcttacc gtatgaaagg taatgaacgt ttttatttaa ttaccgatgc aatgcgtgca
     7741 aaaggtatgc ctgaaggaga atatgatttg ggtggtcaaa aagtaactgt tcaatcgcaa
     7801 caagcacgtc ttacaaatgg tgcacttgct ggtagtattt taaaaatgaa tcatgggtta
     7861 cgtaacttaa tatcatttac aggtgataca ttagatcatt tatggcgtgt aacaagttta
     7921 aatcaagcca ttgcattagg tatcgatgat agaaaaggta gtattaaagt aaataaggat
     7981 gcagatcttg ttattctaga tgatgacatg aatgtaaaat ctacaataaa acaaggtaag
     8041 gttcacacat ttagctaata gataatcata attaaatgta tgcaatagat ttaatctgtt
     8101 aacataagca ctttatatta tgataaaata gaggcaataa catttttttc tgggggtgtc
     8161 taaatgggaa ggcgataaca tgtagttgta atttaagtca tagtgataaa tttgaatgcg
     8221 tgttacccat gagtgacaca tataacatgg aggtgaatcc ctagaaatag ggaattaatt
     8281 ggaaacttcg accataatta gtttgattat atttattcta ttaattgcat taaccacagt
     8341 atttgttggt tcagaatttg cattagtaaa aattagagca acaagaattg aacagctagc
     8401 agatgaagga aataaacctg ctaaaatagt aaaaaagatg attgctaatc tagattatta
     8461 tctttctgct tgtcagttag gtataactgt aacatcttta gggttaggtt ggcttggtga
     8521 accaacgttt gaaaagctat tacacccgat atttgaagca atcaatttac caactgcatt
     8581 aacgacgacg atttcgtttg cagtatcatt tataatcgtt acgtatttgc atgtagtact
     8641 tggtgaatta gcgcctaaat ctatagcgat tcaacatact gaaaagcttg ctttagtata
     8701 tgcaagacca ttgttctatt tcggtaacat tatgaaacca ttgatttggc tgatgaatgg
     8761 ttctgcacgt gttattatta gaatgtttgg tgtaaatcct gatgcccaaa ctgatgcaat
     8821 gtcagaagaa gaaatcaaaa ttattattaa caatagttat aatggtggag aaatcaacca
     8881 aactgaattg gcatatatgc aaaatatctt ttcattcgat gaaagacatg caaaagatat
     8941 aatggtacct agaacgcaaa tgattacact aaatgaacct tttaatgtag acgaattact
     9001 tgatacaata aaagaacatc aatttacacg ttatccaatt actgatgatg gtgataaaga
     9061 ccacattaaa ggatttatca acgttaaaga atttttaact gaatatgctt ctggaaaaac
     9121 gattaaaata gcaaactata tacacgagtt gccaatgatt tcagagacaa cacgtatcag
     9181 tgatgcatta attagaatgc aacgtgagca tgtacatatg agtcttatta tagatgaata
     9241 tggtggaacg gcaggtattt taacgatgga agatatttta gaagaaatcg ttggggaaat
     9301 tcgtgatgaa tttgatgatg atgaagtgaa tgatatcgtt aaaattgata ataagacatt
     9361 ccaagtaaat ggtagagtac tattggatga tttaactgaa gagttcggta tagaatttga
     9421 tgactctgag gatattgata cgataggtgg atggttacaa tctcgcaata caaacttaca
     9481 aaaagatgat tacgtggata caacgtatga tcgctgggtt gtttcagaaa tcgataacca
     9541 ccaaattatt tgggtaatgt taaactatga atttaatgaa ccgagaccta ctatcggaca
     9601 gtctgatgaa gatgaaaaat cagaatagat aataatatat aaaccaacta agaatgattt
     9661 aattcatttt tggttggtta tttttttgac taaaattaat gaaaagtgaa aatagtattg
     9721 gaactcaata tctttaatga atatttttta ttgaaagcga taattcgtat taattgagtt
     9781 tgttgaaaaa tttagggtaa tgtaaagata taaaagatac atagattgga gagatataaa
     9841 gatgttgaat gagatacaaa tattaaataa tggatacccg atgccttcag ttgggttagg
     9901 tgtttataaa atttctgacg aagatatgac taaagttgta aatgctgcaa ttgacgcagg
     9961 ctatagagcg tttgatacag catactttta tgataatgag gcttcactag gacgagcatt
    10021 aaaggataat ggcgtcgata gagaagattt gtttataaca acgaagttat ggaatgacta
    10081 tcaaggttat gagaaaacat tcgaatattt caacaaatcg attgaaaatt tacaaactga
    10141 ttatcttgat ttatttctaa tacattggcc ttgtgaagca gatggtctat ttttagaaac
    10201 atataaagct atggaagaac tttacgagca aggtaaggta aaagcaatag gtgtatgtaa
    10261 ttttaatgtt catcatctag aaaaattaat ggctcaatca agtatcaaac caatggtgaa
    10321 tcaaattgag gtacatccat attttaacca acaagaatta caagaatttt gtgatcgtca
    10381 cgatattaaa gtgactgcat ggatgccttt gatgagaaat agaggactac tagacgaccc
    10441 tgtcattgtt aaaattgctg aaaaatatca taaaacacca gcacaagttg tattgcgttg
    10501 gcatttagca cacaatagaa ttattattcc aaaatctcag acacctaaac gcattcaaga
    10561 aaatatagat attttagatt ttaatttaga attaacagaa gtagctgaaa ttgatgcttt
    10621 aaatagaaat gcaagacaag gtaaaaatcc agatgatgtg aaaattgggg atttaaaata
    10681 actggatgtt aaattttacg tttatgaatg ccttttaatg tgtacattaa aataaatgag
    10741 ttggttttta ctatttgata aaacaatact caggtacatt caaaatcttt taaataaaaa
    10801 ggatggacat agatgaaaat tagagtcgtc attccttgtt ttaatgaagg ggaagtcatt
    10861 acacaaacac atcaacaatt aactgaaata ctttcacaag atagtagtgt gaaaggctat
    10921 gattataata tgcttttcat agatgatggt agtacggata ccactataga tgaaatgcaa
    10981 catcttgcca caatagatag acatgtcagc tttatttctt ttagtagaaa ttttggaaaa
    11041 gaagcagcta tgattgcagg ttaccagcat agtactgaat ttgatgcagt catcatgata
    11101 gattgtgatt tgcaacatcc acctgaatat attccgaaaa tggttgaagg ttttatggaa
    11161 ggctatgatc aagtgattgc aaagcgtgat agaagtggtg aaaattttag tcgcaaaaca
    11221 ttaagccatt tgtactataa gttagttaat tgctttgtag aagaagtaca atttgatgat
    11281 ggtgttggtg attttagact tttaagccaa agagctgtta aatccattgc atcacttgaa
    11341 gaatataatc gattttcaaa agggttattt gaatggatag gatataatac taaagtgttt
    11401 acgtatcaaa atgttgcgag acaaaaagga gaatctaaat ggtcctttaa aaagttattt
    11461 aattatggta ttgatggatt gatttccttt aatagtaaac ctttgagaat gatgatatat
    11521 cttggcttat ttattttttc aataagcgtg ttatatatta tctatttatt catcaatatt
    11581 atgatttctg gtgttaatat cccaggatat ttttcaacga ttgcagctat tttattatta
    11641 ggcggcatac agttaatttc aattggtgtt gtaggtgaat atattggcag gatatattat
    11701 gaagttaagg cacgtcctaa atacattatt caagctacaa atctttcaag tattgaaaat
    11761 gatgagaagg atacccataa agtttattct aaataaacaa aaaaagaagc cctcattaat
    11821 gggagcttct ttttagtctt tgcagtttat tttataaata aatcggatta tgtcgtaatg
    11881 tctaatttgt gtaatgttac agtcatcgta gttcctacat ctatatcact gcttacactg
    11941 atttttgcgt tattttgttg cgcgagttca ttagctatat ataagcctaa tccagaacca
    12001 cccgtttttg tattacgaga gttttctact ctgaatgtac gttcgaatat acgttcttgt
    12061 agttctggta taatgccaat accttcatcg ctaatagcaa tgtcgatagt atcttgatct
    12121 ttgttttcac taatattaat atcaatgcga ctaccaacat ttgaaaattt tagcgcatta
    12181 tcaagtaagt ttgttaaaat acgctcaagt ggcgttcgat attgataaaa tgcatcaatt
    12241 tcgctacaga aattcacttc taatgtgcgg ttttcatgtt tgatgcgttg ctcatatggt
    12301 tgcaatattg atacaagtaa ttggtctagt tgtattaatt ctgggggata tgttttacct
    12361 gtatttaaag tgataatatg agtcatatca tcaaataatg ttgataatct gtttgcttgt
    12421 ttaattaata tgtcgtatga ctctttaatc tcatgatcct ttgtgattat accatcacgt
    12481 agtccttcag aatatgaaat aatgcttgct aaaggtgttt ttaaatcatg ggctaagttt
    12541 tgaatcagtt ctgttttttc ttgttgttcg gatttaattt gattcatttg ttgcgtaatt
    12601 tcagaagcca ttttattaaa agattgattt aattcataaa tttcttttgg tgaattaaac
    12661 gttttatcat tgcttgcgta atttccgtta gcaaattgct tagtttttat attaaactgc
    12721 ttaatttttt gtataagtgg attaataaaa atactacata ttaataaggt taaacagctt
    12781 gtaattattg tcgttaaggt caaagttagt gtcatatggc cgttaaacca cattaaaata
    12841 tatgcaattg ctaaaatagt tgaagttaat agtatactcg atacgacgcc aataatgatt
    12901 tgacttctaa ttgataacac cattatcggc tcctttcaaa tttatatcct aatccccata
    12961 cagttgtgat ggtatatgtt gtaaagctct ctttttctaa tttttctcta atacggtgta
    13021 tatggacatt cacggtatta gcatcttcgt aatagtcata tccccaaact ttttcaagta
    13081 attctgattt agaaataact tcattttctc tagaagctaa ataccacagt aactcaaatt
    13141 ccttaatacg catagggact tcatgaccat ttacagtcac aactttactt aagttaataa
    13201 gtgttaattc atcaaatgac agttgttcaa ctggttgatg atggtatttc ttcattcttg
    13261 taagtaaatt attaatacgt aaaacgagtt cccttggact aaatggtttt ttgacatagt
    13321 catctgcacc taaagttaag gcgtaaatgg tatcatgttc ttgtgttttg gcagttaaat
    13381 agataaaggg gatatctaat ttttgccttt tcatttcttt gacaatgtcg taaccattaa
    13441 cttctggcat catgatatca agtaccatga tatcaatatc atttgatagt aaagaaattg
    13501 cttctttacc gctagttgtc gttgttactt tgtaaccttc atattcaaaa taggtttgac
    13561 aaatgtctac aatgtcttgt tcatcatcca cgatcagtaa gtgggtcatc tattttttca
    13621 cctctgttct tacgacctct aaagtaatta atgatttctt taagtgaaat ctgttttaac
    13681 aatgagtgac tcattaataa aaggataaag aaagttaatt gaagaggata cgtaaatatc
    13741 atatctgcta agatataatt tatcataaca aaggctccaa agaaactagc agcatatgca
    13801 aaaactccaa aaattaaacc taatccaatt gcaatctctc cgagtgggac aacaatatca
    13861 aataatgacg tcgtatgtgc aactatattt gcgaaaaacc acttatacca ctctggtgaa
    13921 tcagtattgt tagcgatgac tggtactaaa cctttcagcg taaatccgcc cgttaatttt
    13981 tcgtaacctt gcattaacat aacaatacct gaacccacac gaatgataaa tgtaacgagt
    14041 agcaataatt tattcatgat gttcacattc tttctattta ttgtgtgtaa tttatataaa
    14101 cataagattt agcaacataa tgtgatttgt agtgttatgt gaacaggaag tgtttgagct
    14161 catgggttgc ttaagctaaa caaacctcaa gctctaaaaa aatttactta tgtgctctac
    14221 aatcttatgg gatttaatag ttgtatatat attgaaaaaa ggaaagtatg atttctacaa
    14281 gctagggggg ctgtgaaatc atacgctttc cattgagtaa tgagtgtaat gtatattttt
    14341 aaaatttgta atgtatagta atagtatgtt gtaaataaaa gtttaactca gaaattggtt
    14401 attttaattt agcgccgccg aagatgacgt gagctttttt acaatagatt gttacttcat
    14461 catatttgct taaatctaca tttttaagat caaatgtttg tttttcttta tcgtagtcaa
    14521 ccattgcgat ttctttaccg tttttaatgt cgccattttt tgttaggtag acgtataaat
    14581 ctggaccttt tgatgatttg tagttagtaa gcattaattt accattttta atctcagctt
    14641 taccttcaac agtttcaccg tttttagaac tgaatgtacc tgttaggtgt tttgttttat
    14701 cagttttaac attgctatct tctgactttg ttttttgttc agttttgtta ccttgatctt
    14761 gtgaattaga attaccacaa gcgcctaaag ctaatgttgt gataatagca ccagctgcta
    14821 aaaaatattt tgtattcatg ctaactcctc atttcttcaa tttgataagt taagtttaaa
    14881 atgaaggcaa ataatatgcc attaactaat tcttaacttc gtttaaatat cgcttaacta
    14941 atattgaaga aagaataatt tgaaaatgaa aataattgtc tgatttaaat aaataggagt
    15001 caaaataaga ggagggcggt tttttaagtg agaaaatatt tatctaaact agttatcact
    15061 ataaaacact tctgatatag cgaactaaga atatcagaag tgttttttag ttttggtatt
    15121 aaaatttata aaccttttgc cacaataaaa acaacaagaa aataagatag acgattaaat
    15181 acaagtacca atatggtgtt aaaaccatca taacgataaa tgaaatgtta ataatgataa
    15241 gtcctttaat cattagtttc tttattttat tattattttc agcagattca tctaagctat
    15301 taaatgttga aataaataac ataataccac caatgatgaa aatggcaggg gctaaaaatg
    15361 gaatagacat atatattgat tgtgtacttg aaaagtcggt aacacgttgc gttaaactga
    15421 caacaatcgt taatatgata gcaataaatt tttggtctgt aatgtataca ggttttaatt
    15481 ttttatcaat gtaaaactgt agtatcgtcc aaatgataaa catcgttata gtacacagag
    15541 taataaagtt atatttatca attatgaatg tatttataaa ggcgtttata gtaatagata
    15601 acaacattgc acttaatgat aaatgttgca tttgatgcct atataaattg aatccagtta
    15661 taagtacgac gatagcaata ttaattaata tatatacaat catgatggac tcctgttcgt
    15721 tatacttcaa tcaactatta tatcaattaa tatattgtta ttcattagat atgacttgta
    15781 aaaagtaaaa taagttatcc tttatacacc ttttttatta ctccaaagta atgtatgaag
    15841 ttgtggtaac acataaacgt gattcatatc attactttgc ataactaaat ctaccaactg
    15901 ctcgtagcgt tctaacaact tttcggtatg attatctacg ctgtctgata aatatgggtt
    15961 accaacttgt aaatagaagg gaatatctgg ataacggtgg tgtatcattt tggcaaaatc
    16021 ataatcttta tcgtcgaata caactacttt taagtttaat gaggaaggta cgcattgtgc
    16081 aatcacttca tctaactttt ttaaatcagg cgtcatagtt gaacttggtg gttttggact
    16141 aatcgttaaa tcatcaattt gtgtcatcca aggttggaat ttactgcctt gtgtctccag
    16201 tgcgctgaaa atacctttat cttgaaataa gtcaactaac tcttggatac ctttaattaa
    16261 tgctgggtta ccaccagaaa ttgtaacgtg gttaaataaa tcgccaccaa ttcgttttaa
    16321 ttcatcataa atttcttcag cggtcatgag ttttatatcg cctttagcac taccatccca
    16381 agtaaatgca gaatcacacc agctacagcg ataatcacat ccagctgttc tcacaaacat
    16441 cgtttttcta ccgattactc gaccttcacc ctgaatggtt ggaccgaata tttcgagtac
    16501 aggaatttta gccattagtt acacctgttc ctttggtcta aatacgacat aacttgttgg
    16561 tgtttctctt acaaatactt gaatacattt tggttggtgt tcgagcgatg ccaaattttc
    16621 tttaacaatt tgataaattg tttccgctac gatttcagtt gaagggattt tgtttttaaa
    16681 agcaggtaag ttatttaaca gttgatggtc aaatttaccg tgtatcatct ttttcaaatg
    16741 gctaaagttc actaaaaagc cagtgtcatc tagtttatca ccgacaattg ttaaattaac
    16801 aaagtaagta tgaccatgga cattttgaca aatacctgct tcttcacacg gaatgtgatg
    16861 tgcagctgaa aaattaaaat ctttatttaa ttcgaattga tatggatgcg ttgtactagg
    16921 atagatttgt tgtaacattt taaagcgctc ctttactttc aagatattga tttagtccac
    16981 gttgacgtaa atgacaagct ggacattcac cacagccatc cccaatgata ccgttatagc
    17041 atgttaatgt ttttgtacga atataatcta aaacttcgag ttcatcactt aatttccacg
    17101 tttctgcttt gtttaaccac attaaaggag tatgaataac aaaatctttg tccatagcta
    17161 ggcttaatgt tacgttcatt gattttataa aactatcgcg acagtctggg tagcctgaaa
    17221 agtctgtttc acatacgcct gtaataatat gcttagcccc aatttgataa gctagagcac
    17281 ctgcaaacga caagaaaagt aaatttctag ctggaacaaa tgtattaggt ataccatctt
    17341 cattattagt aatttccata tcatgttgtg ttaatgcgtt tggagtaagt tgtgataata
    17401 atgacatatc taaaacgtga tgtttcattc cttgatcttg tgcaatttgt tttgcgactt
    17461 caatttcagt atcatgtctt tgaccataat taaacgttac gagttcaact tctttgaaat
    17521 gtttttttgc ataaaagaga catgttgtac tgtcttgacc accactaaag acaacgatgg
    17581 ctttttcatt atttaataca ctttccattt tgtaattgct cctatcatta ataatattaa
    17641 taaagaggtt aatggcattg ataagcccgt ttttaattta taaaataaaa aaaagcctat
    17701 ctccataaaa gatagacgaa agaaatgggt tgctcctata atatatatta aaggtttacc
    17761 aacgaatgtt tgaaatttca atgtctagtt ttttatagag ggtttcagct aggaacctct
    17821 gatattcatt tatgtacgat gaatagaata taccatatag tagttaaaat caaggtcagg
    17881 ataatgacaa tgcctttaaa gttttagcta catccaatac atattataaa aataaaacac
    17941 atttagaacg gagtatacaa tgattctagt catagataat aatgattcat ttacatataa
    18001 tttaatagac tatattaaga ctcaaacgaa actaacagtt caagttgttg gtattgataa
    18061 tctgctgata gaagacatca ttaatatgaa gccaaaagca attgttattt cgcctgggcc
    18121 gggtaatccg gatgattatc ctatcttgaa tgaagtgtta gaacaatttt atcagcgtgt
    18181 acctatacta ggtgtatgtt taggatttca atgtatcgtg tcttattttg gtggaaatat
    18241 cattcacggc tatcatcctg tacacggaca tactacacag ttacgccata ccaatgaagg
    18301 tatttttcaa ggactgcctc aaaatttcaa tgtaatgcgt tatcattcat taattgctga
    18361 cggagcgact tttccaaatt gcttaaagat tacagcaaaa aacgatgaag caattattat
    18421 ggcatttgag catattacat ttccggtttt tggtgtgcaa tatcatcctg aatctatttt
    18481 gagtgaatac ggttatcgac aagttgaatt atttttatcg aaggtaggtg attactgtga
    18541 gaatagaata taattatcgc tactatttaa ctgaaaatga atataagcaa taccatattc
    18601 aattaaaggg atttataaag aagtatgttg ccactaagtt ggctgatgtg ggagaagtga
    18661 tacactttgc acaagcgcag caacgacaag gtagatatgt ttcgttatat ttaagttacg
    18721 aagcggcaaa gtattttaat catgttatgt gtacacattc attagctaaa gatgatattt
    18781 atgcagtagc ttatagtttt gaaaatgcgg aaagcataaa ttcaacatat gaacatcaaa
    18841 ctacttatgt atcaaagcat catttttcat ttgttgaatc ttctgaggtt atgatgacta
    18901 atattaaacg tgtccaacaa gcaattgttg aaggcgaaac gtatcaagtg aactatacgg
    18961 cgcgcttaac ggacaacatt tattatccta ttagtacttt atatgaacga ttaactcaat
    19021 ttagtaatgg taattatact gcgttattac aaacagatga aatccaagta gcgtctatct
    19081 caccagaatt attttttcaa aaaggacagt ttaacaatgt cgataacgtt atcataagca
    19141 aaccgatgaa agggacaatg cctagaggta aaacggaagc tgaagatcaa cagtattata
    19201 aaacattgca aacttcttcg aaagatcgtg cagaaaatgt catgattgtt gatttactaa
    19261 gaaacgatat agggagaata tcacagagtg gctcaattaa ggtgtataaa ctatttttca
    19321 ttgaggtata taaaactgta tttcaaatga cttcgatggt aagtggaact ttaaaaacta
    19381 atacagactt aactcaaatt ttaacatcgt tatttccttg tggttcgatt acaggtgcac
    19441 cgaaactgaa tacaatgaaa tatattaaac aattagaaag ttcacctcgt ggtatatact
    19501 gcggagcaat tggactatta cttccaactg aagatgataa aatgattttt aatattccaa
    19561 ttcgtactat tgagtataaa tatggacaag cgatttatgg agtcggagca ggtattacaa
    19621 ttgattctaa gccaaaagat gaagtaaatg aattttacgc aaaaaccaag attttggaga
    19681 tgttataatg caattatttg aaacaatgaa aattgataat ggacatatcc ctagaattac
    19741 ttatcatact aatcgcataa aatgttcttc tgagcgatta aactttaaat ttgatgaaca
    19801 tgcatggcga aatgaattaa acgatgtaac aacaaagtat cacaatggcc aatatagact
    19861 taaaatcgta ttacatgctg aaagcgaatt cgaaacgata gtgtcacctt tacctgagaa
    19921 aagtagtttt acagcaaaat ttcaagtgtt gcccaaagca gttaatccaa cttttataaa
    19981 taataaaacg acagaacgaa agcatttagc acacaatcat gaaacagatt taatattgct
    20041 aacttcagag gacggcaagg tccttgaatt tgatattggc aacattgtca ttgaagagga
    20101 tggaaaatgg tacacaccaa gttataaaga tgatttctta aaaggatgca tgcgtgatta
    20161 tttaatagat agtgacaaac ttgttgaaaa agactttaat aaaaacgaat tgatttataa
    20221 atatcataac aatgagatac gtatattttt gataaatagt ttacgagagg ttgccgatgt
    20281 ccacctttgc ctttaaaatt agtatgataa taatgtagaa aatgaagtta aaacaaattt
    20341 aaaaattaaa tggggccaaa tatgattatt gtttatattg tgctgttgtt aattcttgta
    20401 tacgtaaatt atcgattagt gaatcgattg ctatctgaaa atagaatata tgttgttcgt
    20461 ttgatagcaa caattactac tgttataagc tttatccttg tatacgcatt aattcacgaa
    20521 ctcatgcctt ttgttgtgcg agcaatggat ttattgtatc accagtaaat ttaatgagta
    20581 ggtaaaatga actattcaat cgattctata attaaaaatc aaagtaaagt aaccaaatat
    20641 tgaagaggtg tacaaatgaa aatatatagt caaggtgacc aagccattgt agtagcaatt
    20701 gaaaaagaag tatctaaaag tttaaccgaa gatttattaa cacttcgctc atatttaatt
    20761 gaacaaaatt atccatttat tatagaaatt gtgccatcag aatcagacat gatgattgtc
    20821 tacgacgcaa gggatatgat taaacaccat aatatacaat cacctttttt atacatgaaa
    20881 gcactaatag aatcgattca tttaaacata aaacatgatt ttaaccagca agatttgatt
    20941 gaaataccaa ttgtgtatgg ttcgaaatat ggtccggatt tagaatcact tttaaaacat
    21001 tacaaaatca agctagaaac atttattgaa ttacattcta aggcgcaata ttttgtttcg
    21061 atgatgggat attcacctgg gtttccttat ttaactggat taaataagaa attgtatatt
    21121 aatcacacga gtaaacagaa aaaattcatt ccagctggtt ctgtagtact tgaagggaaa
    21181 aaatgcggta ttgtaactac ggatacaatt aatgattggt tagttattgg ttatacacca
    21241 ttatcacttt ttaatccgaa agaatcagat ttcgcacgct taaagttagg ctataatatt
    21301 aaatttagac ctatcaatga aaatgaatta gaagtaggag cgtttaaaga tgtcaatcat
    21361 aattgaaaaa agtggcttat tcagtagctt tcaggacttt ggtagaaggg gatatgaaca
    21421 tgatggtgta attccatgtg gtgcacttga tactttagca catgaaattg ctaatcgatt
    21481 agttgcaaat gacaagaatg aagcaacttt ggaaatgact aataaaatgg caacgattcg
    21541 ttttacagaa cctacgctga ttgcattagc agggggtaat gtcaaagctt acactgagca
    21601 aatgactata tctccatata aattgtattt gttagataaa ggcgatgttt taaagtttag
    21661 agaaacaagt tatacatcgc gagtgtattt agctgtggga ggtggatttg aattagatgc
    21721 atggttagga tctaattcaa ccgactttaa tgtaaaaatt ggtggtttta aaggaagaac
    21781 attacaagat ggcgatgaaa taaagcttaa gagagattat acagctcgtc atcataagtt
    21841 atttgaaaac cttgctcaca cgaaacaaac agattggggt attgatgggt acgccttgtc
    21901 atttaattat atgtctgatg tatttcatgt cgttaaaaat aaaggtacgg aagattttaa
    21961 agaagatgcc attcaaagat ttgtgaaaca tgattataaa gtaacgagca aagcaaatcg
    22021 catggggatg atgcttgaag gtgaaaaaat caaagctttt tatgaagata tgccaccgta
    22081 tcagactgtc aaaaaaggaa cgatacaaat taagcgtgat ggcacaccta ttatcttatt
    22141 aaatgatcat tacacgttag gtagctaccc gcaaatcggt acaatcgcaa gttatcattt
    22201 aacgaaatta gcacaaaaac cgcaaggatc acgtttgaaa tttcaattta tagatatttt
    22261 aacggctgaa aagaacctcg ttaagtatag taactggtta aaccaattat tccatggaat
    22321 agaatataga atgcaattag aaatgatgaa ataatatttg gtacgtagct catactagag
    22381 tccgatgcaa ataatttcct cctaatgtat aatgaaataa tactgtgttt tatctgcgaa
    22441 atgtattata ttctaattcg tttcacagta aaatgaaaag ataaagtgtg tttttacttg
    22501 aattttgact aaaattactc ta
//
LOCUS       DAKSQE010000035        22134 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.035, whole genome shotgun sequence.
ACCESSION   DAKSQE010000035 DAKSQE010000000
VERSION     DAKSQE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22134
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.035"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(307..668)
                     /gene="ssrA"
                     /locus_tag="O0V33_001171"
     tmRNA           complement(307..668)
                     /gene="ssrA"
                     /locus_tag="O0V33_001171"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(759..1223)
                     /gene="smpB"
                     /locus_tag="O0V33_001172"
     CDS             complement(759..1223)
                     /gene="smpB"
                     /locus_tag="O0V33_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCX8975817.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIIKLGDQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            complement(1245..3617)
                     /gene="rnr"
                     /locus_tag="O0V33_001173"
     CDS             complement(1245..3617)
                     /gene="rnr"
                     /locus_tag="O0V33_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCX8975818.1"
                     /translation="MNLKQSIEEIINQPEYESMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERSKTDRYQKKHSSKGQSKLIKGTLSQNKKGFAFLRPKDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLEEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGHTQLTVSIADVSYYVTEDSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPHVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPNIREQY
                     KEITPMLDLAQDLSNRLIQMRKRRGEIDFDISEAKVLVNEDGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLDVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIESERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGHKKKGKQRKGKNQRKNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            complement(3651..4391)
                     /locus_tag="O0V33_001174"
     CDS             complement(3651..4391)
                     /locus_tag="O0V33_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCX8975819.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPEEILQSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKDQETIDKEMHHFKPTET
                     LKELSEALDTIKDQVDEVLDPILVIQAENDNMIDPQSANYIYEHVDSDEKDIKWYSQS
                     GHVITIDKEKEQVFDDIYQFLESLDWSE"
     gene            complement(4520..4753)
                     /gene="secG"
                     /locus_tag="O0V33_001175"
     CDS             complement(4520..4753)
                     /gene="secG"
                     /locus_tag="O0V33_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCX8975820.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            complement(4820..5278)
                     /locus_tag="O0V33_001176"
     CDS             complement(4820..5278)
                     /locus_tag="O0V33_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975821.1"
                     /translation="MADRTNKEIKTGRFIATASIAFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
     gene            complement(5616..6920)
                     /gene="eno"
                     /locus_tag="O0V33_001177"
     CDS             complement(5616..6920)
                     /gene="eno"
                     /locus_tag="O0V33_001177"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCX8975822.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
     gene            complement(7050..8567)
                     /locus_tag="O0V33_001178"
     CDS             complement(7050..8567)
                     /locus_tag="O0V33_001178"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCX8975823.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVEDQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
     gene            complement(8570..9331)
                     /locus_tag="O0V33_001179"
     CDS             complement(8570..9331)
                     /locus_tag="O0V33_001179"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCX8975824.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            complement(9453..10643)
                     /locus_tag="O0V33_001180"
     CDS             complement(9453..10643)
                     /locus_tag="O0V33_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCX8975825.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            complement(10782..11792)
                     /gene="gap"
                     /locus_tag="O0V33_001181"
     CDS             complement(10782..11792)
                     /gene="gap"
                     /locus_tag="O0V33_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCX8975826.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            complement(11845..12858)
                     /locus_tag="O0V33_001182"
     CDS             complement(11845..12858)
                     /locus_tag="O0V33_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCX8975827.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKDYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            complement(13352..13981)
                     /locus_tag="O0V33_001183"
     CDS             complement(13352..13981)
                     /locus_tag="O0V33_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HCX8975828.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATILLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDKASQDKSDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
     gene            14683..15585
                     /locus_tag="O0V33_001184"
     CDS             14683..15585
                     /locus_tag="O0V33_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCX8975829.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNNKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKVLFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGIILSNEGGALQTMKLPYEYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWVPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLISK"
     gene            complement(15792..17426)
                     /locus_tag="O0V33_001185"
     CDS             complement(15792..17426)
                     /locus_tag="O0V33_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCX8975830.1"
                     /translation="MKGIELLNNPFLNKGTAFTNEERKQLGLEGLLPANVRTLEQQAE
                     QCYEQFKAKQTDFEKRLFLMAIFNRNRTLFYKLTSEHLVEFMPIIYDPVIAQSIEQYN
                     ENFSRPQDAVFLSVDRQDQIEQELRNVANGRDIRLIVVTDAEGILGIGDWGVDGVDIA
                     IGKLIVYTAAAGINPAQVLPVSLDVGTNNEQLLNDELYLGNRHKRVDDETYYTFVDKF
                     VSAVRKEYPNALLHWEDFGRGHAKNILDKYEDTLPTFNDDIQGTGIVTLAGVLGALNI
                     SKVDYTNQTFLVYGGGTAGMGITNILKDELIKQDVSEDKANQHFYIMDKQGLLFDDMD
                     DLTEAQQVFAKNRNEFSNTVNWKNLKEVIENIKPTVLIGTSTQTGAFTEDAVKEMLKH
                     TERPMIFPLSNPTKLAEATAQDLLKWTNGKALIGTGIPYNDIEYNGVNYSIGQANNAL
                     MYPGLGLGLIASKAKKVNQQILSAASHALGGMVNPDEPGAAVLPPVEKIHQCSRAIAK
                     AVAQSVIDQGLNAETIDDIDEAIEAEIWYPEYKSYI"
     gene            complement(17608..18195)
                     /gene="clpP"
                     /locus_tag="O0V33_001186"
     CDS             complement(17608..18195)
                     /gene="clpP"
                     /locus_tag="O0V33_001186"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCX8975831.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
     gene            complement(18764..19708)
                     /gene="whiA"
                     /locus_tag="O0V33_001187"
     CDS             complement(18764..19708)
                     /gene="whiA"
                     /locus_tag="O0V33_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCX8975832.1"
                     /translation="MSFASEMKNELTRIDVDEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     ELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
     gene            complement(19817..20812)
                     /locus_tag="O0V33_001188"
     CDS             complement(19817..20812)
                     /locus_tag="O0V33_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCX8975833.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            complement(20809..21720)
                     /gene="rapZ"
                     /locus_tag="O0V33_001189"
     CDS             complement(20809..21720)
                     /gene="rapZ"
                     /locus_tag="O0V33_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCX8975834.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKKSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
BASE COUNT         6715 a         4263 c         3210 g         7946 t
ORIGIN      
        1 gattctaatt ctaaactata tatttaattc tcttatttat gatacttctt tagcttaatt
       61 aattttgcaa ataaaattta cattttaaaa ctcaaagctc aacttttttg acacttattt
      121 gacacatatt ctttcaaccc ttaacgcaaa atcttatatg tacacaagta ttaatttttc
      181 attcatgaaa atttgaacct aatcagatac tatcatatca acgttcacag ctaatttaac
      241 cttcctcaca agaagagata aaaaacaaat aaaaaagccc acatccgcaa aggttgtagg
      301 ctacaaatat ggagacggcg ggatttgaac ccgcgtccag aggtcctgat acacatcttt
      361 ctacgtgtgt agtttatcga aaagtttcgc atcatgaaaa gtgataaaca accaacatga
      421 tgctagcttg attaagtttc ttctaaacag acttcaaacg gcagtgttta gcatatccta
      481 ttaaggttga atcgcgttaa cagcacatag gaaatgctgt taggcgatgc agagtgcgat
      541 taggcagcta ctgcgaaatt attgtttgat ttgccagtta ttataaactg tgtgtgttga
      601 tgacgaagac aaccctccga cacgcttaat gagctcgggg gacccctgtc gaatccagaa
      661 acgtccccga aataataaat caaacaaatc atacttttgc aatttacaaa agcacatata
      721 actatatcaa atatagcccg attaagcaac taaatcgctt aataacgggc tttcatatcg
      781 cgcgcaacat ctcgtttgac tgctttttct ttcaaagctt gacgtttatc atattttttc
      841 ttacctcgtg caacaccaag taatacttta caatgtccat gcttcaaata aagctttaac
      901 ggcacaatcg aataaccaat ctcacgtgtt tgatcaccca atttaatgat ttcacgctta
      961 tgcaataata attttcgaga acgaagagga tcgtgattaa aacgattccc ttcttcgtat
     1021 ggtgctatat gcatattatt caaatacatt tcaccgtttt taacttgcgc ataactatct
     1081 ttaaggttag cactacctcg gcgaattgat tttatttctg tgccttgcaa tacaattccc
     1141 gcttcaatcg tgtcttcaat attataatca tgtctagctt tacgattttc cgctaatgta
     1201 cctggtgatt tcttcttagc cattcatact cacctcattg ctgcttattt tttcttacga
     1261 cgtgctttct ttttcacact tttatcttta taaaatggct tatgtttact gtttcctgat
     1321 ttgtcatttt tacgttggtt tttaccttta cgttgcttac cttttttctt atgaccttta
     1381 tcatcagatt tcgatttatc taatgattta ccacgcgttt tagcttgaat tgtcttacct
     1441 cgtgctgggc gctgtgatcg atcatttttc ggtaaaggca tacctacaat ttgaaaatca
     1501 attaatcgtt catctacatc aacatgcgtc actttaacct taactgtgtc accaattcta
     1561 aatactttag cttgacgctc accaattaat gccatttgac gctcttcaaa acggtaataa
     1621 tcatcagtca tattcgcaat atgaaccata ccttctatcg tatttggcaa ttcaatgaac
     1681 ataccgaagt tagctactga gctgacaata ccttcaaatt catcaccaat atgttgaatc
     1741 atatattctg cttttttcaa ttcatcagta tcacgttctg actcaatagc acgacgttca
     1801 cgtttagaag tatgttcagc taactcaggc aatttgtctt cccaacgctt cacttctttg
     1861 ttatccattg atttctcaat taaatactta cggattaaac gatgaactgt taaatcagga
     1921 taacgtctta ttggtgatgt aaaatgcgta taatattcag ctgataagcc aaaatgtccc
     1981 aagttcacat catcataatg cgcttgttgc attgaacgta acatcattgt tgaaatgacc
     2041 atttgttcag gtcgaccttc tacttcttct tggacctttt gaagtgttgt tggatgaata
     2101 tcttcgcctg ttcctttaat cataatgcca aagttagtaa taaaatcaaa gaattgtctt
     2161 aagcgatctg atttaggttg ctcatgcact cggtaaataa atggtacatc taacttacta
     2221 aaatgttcag caactgtttc atttgcaatt aacataaatg actcaataag acgttcgcct
     2281 tcaccacgtt gtctcaattg aacatctgta ggtataccat cttcgttaac tagtactttc
     2341 gcttcgctaa tatcaaaatc aatttcacca cgacgttttc tcatttgaat caaacgatta
     2401 gataaatctt gtgctaaatc taacataggc gtaatttctt tatattgctc gcgaatgtta
     2461 ggatcctttt cagtaataat ctggttaact gcatcatacg tcattcgata atcagaatgt
     2521 ataacactat caaaaatttc atgtttaaca acacgaccac ttgcatcaat ttccatgcga
     2581 cagcttaacg ttaaacgatc aacgtgagga tttaatgaac aaataccatt gcttaatcga
     2641 tgtggaatca tcggaatcac acggtcaaca agatatacac ttgtcgctct atcataagct
     2701 tctttatcta aagctgaatc ttccgttaca taataactga catcagcaat acttacagtt
     2761 aattgcgtat gaccgttcgc taacttttta acactgattg cgtcatctaa gtctttagca
     2821 tccgcaccat caattgtgat tgtcaattca tcacgtaaat catgacggcc tttaatttca
     2881 gtattttcaa tatgatcagg tactgcttca gcttcttcta atacttcatc aggaaactca
     2941 atttcaatac catgttgata aataatagat aaaatatcta cacctggatc atttttatgt
     3001 cctaatattg ctgaaatatg cccttcagga ttatctgaac catcagcata cttagtaatt
     3061 tgaactagta ccttatgccc atctactgca cctaaacttt gacctttagg aatgaaaata
     3121 tcttgcataa tacgtttatc atctggaata acaaagccga aatgtctagc ttcactatac
     3181 gtaccaacaa cttgagttac agaatgcttc tcaatcgact taacttcccc ttcaatttta
     3241 cctttatgtt cacctttaga ttgatgtatt tctacaatta cagtatctcc gtctaaggca
     3301 cgattaattt ttgttggggg aataaatata tcttccatat cctcatcttt aggtcttagg
     3361 aaagcaaaac ctttcttgtt ttgacttaac gttcctttta tcaatttcga ttgacctttg
     3421 gaactatgct ttttttggta tctatctgtt ttcgaacgtt cgattaatcc tgattgttct
     3481 aactccacaa gcaccttaat taaatctcta aacgagtcgg cactgcttaa acctaatgca
     3541 tcttgaaaat ctgaaactga catagattca tattcaggtt gattaataat ctcttctatt
     3601 gattgcttta aattcattat gcccctcctt tctaatgtta aaatctcttt ttattctgac
     3661 cagtctaatg actctaaaaa ttgataaata tcatcgaaaa cttgttcttt ttctttatca
     3721 attgtaataa catgtcctga ctgactatac catttgatat ctttttcgtc agagtctaca
     3781 tgttcatata tataatttgc ggattgtgga tcaatcatat tgtcgttttc tgcttgaatc
     3841 actaaaattg ggtcaagcac ctcatcaact tgatctttaa tcgtatctaa tgcttcactt
     3901 aattctttta aagtttcagt tggtttgaaa tgatgcattt ctttgtcaat cgtttcttga
     3961 tctttaccct catacttttt aaaattgcgt gcatattcta aaaagccttc ataaatggca
     4021 ccttcagttt tgccacccat aggagcacac atcgttacaa taccctttac atctcgattt
     4081 aagcttaatt ttaatgcaaa atcgccacct aatgacaagc cagcaacaac gatttcatca
     4141 taaccttgtt caacaaggta gtcatagcca tctaaggcat ctttaaacca aacaaaagga
     4201 cttgattgaa gtatctcttc tggtggtgct gcatgacctt catattgtgg tgcatacgat
     4261 gtataacctt tcttttgtaa aaatcgacct aattgacgaa catcagacga attgcctgta
     4321 aaaccatgta gtaataacac ggcacgttta ccttcctcaa aaaagaaagg ctttggtaac
     4381 tttatttgca taaatctaac gctccttttt cacaattaac aaacgtactt aattaaacaa
     4441 tatgtttcta ataattgaac atgagtcctt tattattcta attataaata aaaaagcccg
     4501 acatttacat cgccggacct tacataccaa gataacttat gcaaatcata agtacaaaaa
     4561 ataatattga taaaataatt gttaatctat ttaagaataa atcgacgcca cgttgttttt
     4621 gtttaccgaa taactgctca gcaccaccac tgatggcacc tgaaagtcca ctgcttttac
     4681 cttcttggag tagtacaaca gttattaatg caatacaatc aatgattaat aatacgatta
     4741 aaaatgtatg cataaattgt cctccgttcc ttatatacga caagtagtat atcatatttt
     4801 cattgttaaa atcaattttc tagcgattaa atgtactttt acgtttgata gaaacaacaa
     4861 gcatataaat cgctaatgca accgaaccta taataacaat taaaattggc aatgttaagt
     4921 acgcaccgtg tgcagctttt attgcttgaa ttgtgattga taaattaatc aacgtaaaca
     4981 aactatttga tacataaact gcaaacataa accaccacac ataagtatgt cgattccaaa
     5041 aagtgatgaa gcctgctaga taagccaaaa tatacataat accagtgaat ctaaagctgt
     5101 ttaaaatgta atctatcgct ttatctgaag tgttttgatt cgttaaattg agtaatgctt
     5161 tggcagtcgc attatccaac gaaacaaagt aatgaataat caacaatatt gagaatgcga
     5221 ttgatgcagt tgcaataaag cgtcctgttt taatttcttt attcgttcta tcagccattt
     5281 gtttactcct catatattta aatacgttct ataaaacctt tatttaattt taccttttaa
     5341 taaaagtgaa taccctgtca tacaatattc atttcaacat aaatttatta taccagttta
     5401 ataatacaag ttaaatatgt gttattagtt acctcatgat tattagtctg ttatgaataa
     5461 tacttcaaaa aaaaaacctg ccacagtctc aactctgtgg caggttataa ataaaagcgt
     5521 gaacttttta agcgtgaact caaaaacttt attcaattta taccgacata ataatcctca
     5581 actaaaatta tggcagtact taatgtttaa aatgattatt tatctaagtt atagaatgat
     5641 ttgataccgt catatttagc agtttcaaat aattcatctt cgatacgtaa taattgattg
     5701 tatttagcaa tacggtcagt acgtgataat gaaccagttt taatttgacc agcgtttgta
     5761 gcaacagcga tatcagcaat tgttgtatct tctgtttcac ctgaacggtg agaaactact
     5821 gctgtgtaac cagctttttg agccatttcg attgcatcaa atgtttcagt taatgtaccg
     5881 atttggttaa ctttaattaa gattgagtta ccaattccgt tttcaatacc ttttgctaaa
     5941 atttcagtgt ttgttacgaa taaatcgtca cctactaatt gtacacggtc accgatacgt
     6001 tctgtaagtt gtttccaacc atcccagtcg ttttcgtcca taccgtcttc aattgtaatg
     6061 ataggatatt tgtctactaa ttgttctaag tagtcaactt gttctgcagc tgtacgtttt
     6121 gcaccgtgtt cgccttcgaa cttactgtag tcatatacac cattttcata gaattctgat
     6181 gatgcacagt caaatcctaa gaatacttct tcacctggtt tgtaaccagc tgcttcgatt
     6241 gcttggataa ttgtttcaac agcatcttca gtaccttcaa atttaggagc gaaaccacct
     6301 tcgtcaccta ctgcagtttc taaaccacgt ttgcttaaaa ttgattttaa gttgtggaaa
     6361 atttcagtac cccaacgtaa tgattctttg aacgttgtag cacctacagg taaaatcatg
     6421 aattcttgga atgcaattgg agcatctgag tgagaaccac cattaacgat gttcatcatt
     6481 ggtactggta actgcttacc attaaatcca cctaaatatt tgtaaagtgg ttgacctaat
     6541 aagtcagctg ctgcacgtgc tactgcaata gatacaccta aaatagcatt tgcacctaat
     6601 ttacctttgt ttggagtacc gtctaatgcg atcatcattt tatcaataga tacttgatct
     6661 aatactgaaa attcaccttc aataatttct ggtgcgatga tttcattaac gttttcaact
     6721 gctttagtaa cacctttacc taaataacgt gatttatctc catcacgtaa ttcaacagct
     6781 tcgtgttcac cagttgaagc acctgatggt actaatgcac gaccaaatgc gccactttca
     6841 gttaatactt ctacttcaac agttgggtta ccacgagagt ctaagacttc gcgagcgtaa
     6901 acatctgtaa taattggcat gtttataatc tcctttaaat agttattcta taattattat
     6961 agctttattc tttcatttaa caaaatgata ttactattat tttcaaaaaa taagtgtcat
     7021 taagcgttta cttaacatct tttacaatat tagtgtttaa ttaaagattc acctgtcata
     7081 tcttcaggtt gttctacatt taataaatct aataatgtag gtgctaagtc acctaaacga
     7141 ccagtttctc taagtgtaac gccttctttt gttacaatca ctggtactgg gttcgttgta
     7201 tgcgtcgtca ttggttgatc atcatccgtc aatacttgat cagagttacc atggtcagca
     7261 gtaataattg cataaccgtc catgtctaaa atcttatcaa ccacttcgcc taaacattca
     7321 tcaaccgctt cgattgcttt gattgtcggc tcaagcatac cactatgtcc aaccatatca
     7381 gggttagcaa agtttaaaat aattaagtcc aagtcacctt tatttaactc ttctaataat
     7441 gcatctttaa cttcataagc actcatttct ggtttcaagt catacgttgc aactttaggt
     7501 gaatcaatca aacgacgacg ttcaccttta aattcctcat tacgtccacc actcataaag
     7561 taagtaacgt gaggatattt ttcagtttct gcaatacgta attgagttaa attgttattt
     7621 tgtgcaattt caccgattgt attatttaaa tcgacttttt cgaagacgat agccgcatcg
     7681 atattgtcat tatacttagt gaatgttgca tagaataagt ctttaacttg ttcaacttta
     7741 aatccttcga atgctctgtt cgcaaaaatt tccgataatt gtgccgctct atcaggtcgg
     7801 aaattataga agatcactgc atctccatca ttaacaccgt cattttgatc ctcaacgatg
     7861 aatggtacta cgaattcgtc agttaagcct tcattataac ttgcttccac accttctttg
     7921 gcagttgcat aagttggggc atcaaagtta cgaatagcat tgtaagcttt ttcttcacgt
     7981 tcccaacgtt tgtcacggtc cattgcataa taacggccag acacagatgc aaattgacca
     8041 atacctaatt cattgaattt agcttcagtc tcttcgatgt atttcaaagc ggatttttga
     8101 tctacgtcac gaccatctaa aaatgcgtgt acgtaaactt tttcaacacc ttgtttttta
     8161 gcaagttcta acaaagcaaa taaatgtttg taatgactgt gtacaccacc gtcagacaat
     8221 aaaccaaaga tgtgtaacgc tgaatcatgt gaattcacgt gtgcaattgc attatttaaa
     8281 acatcatttt caaagaaatc accgtcttca attgatttat tgattcgagt taaactttga
     8341 taaacgatac gtcctgcacc gatattcata tgaccaactt ctgagttacc catttgtcct
     8401 tcaggtagtc caacatctaa gccactcgct tcgatttgag tcgttggata tttgttgtaa
     8461 taacgatcaa aattaggctt gtttgctaat tttaccgcat taccatgttc gctttcgcgg
     8521 ttcgcaaaac catctaaaat aattaacgca gttggtttct tagccatgat tattttgcac
     8581 cttctaacaa ttgtacgaaa tcttcaactt taagtgatgc gccacctact aatgccccat
     8641 caatatcagt ttgtgccatg tattctttaa tgttgttagg tttaacacta ccaccatatt
     8701 gaatacgagt tgcttctgat acttctttgc ttgataagtc agcaatagtt tgacgtacaa
     8761 atgcacacat ttcattcgca tcttcagatg ttgatgattt accagttccg attgcccaga
     8821 ttggttcata agcaattaca actgatttaa gttgatcttc agataaacct gcaacagctt
     8881 tcttaacttg ctcacctaca acatcgttag ctttaccact ttcacgctct tcgtctgttt
     8941 caccaacaca aataattgga gtcattccat gtttgaaaat agcgtgcgct tttttgttaa
     9001 tttcttcatc tgtttcgtgg aataattcac gacgttcaga atgaccgata acaacgtatt
     9061 taacgcctaa atctgctaat gcaactggag acgtttcacc tgtgaacgca ccgttatctt
     9121 cgaaatacgt attttgagca ccgatttcta aaccttgtgc ttttccttct ttaactgcag
     9181 tagttaatgc atctaattga attgctggtg cacaaattac tgattctact tcttttgaat
     9241 ctggtaatgt tggcaatgca ttgacgaagt cttttgcttc ttgtactgtt ttgttcattt
     9301 tccagttacc agctataatt ggtgttctca ttaaagacac tccttgtttt gtaaatattt
     9361 ttgaaaagtg atgaaacacg atgtcatctt atgactgttt tcccgtaaca atgttaaaca
     9421 aacatgccac atcactttaa actatcactt tattatttat tattgattgc tttgatacca
     9481 ggcaattctt taccttctag gtactctaat gacgctccgc caccagttga aatatgagtg
     9541 aagtcatttt caaaacctaa agagattgct gctgcagctg aatcaccgcc accgataatc
     9601 gtaattgcat ctttaaggtt tgcaattgct ttacatacac caattgtacc ttgtgcaaag
     9661 ttactgaact cgaatacacc cataggtcca ttccatacaa cagtgtgcgc accttctaat
     9721 tcatctgcaa ataattttac agtgtttggt ccaatatcca taccttcttg gtctgctgga
     9781 attgaatcag atggtactac agtaattttg gcatcattag aaaattcttt agcaacttta
     9841 gtgtctactg gtaatacaat tttatcacca tgtttttcta ataaatcttt tgcgaagtcg
     9901 attttatctt cttctaataa tgaaatacca atttctttac cttgcgcttt taagaaagta
     9961 taagccatac ctccgccgat gataatttta tcagcgatgt taactaagtt tttgatgaca
    10021 ttaattttgt cagatacttt tgctccacct aaaatagcaa caactggttt atgtggatcg
    10081 ttaactacgc caccgataaa cttaatttct ttatccatta agaatccagc tgcagtttct
    10141 aagtgtgtag aaataccaac attagatgca tgctcacgat gcgcagtacc aaaagcatca
    10201 tttacaaaca catcacctaa agatgcccag tatttaccta attctggatc atttttagat
    10261 tcttttttac cgtctaaatc ttcataacgt gtattttcaa ctaataatac gtcgccttct
    10321 ttaaggtctt taatagctgc ttcaagtttt tcgccgcgtg tttctggtac gaaaacaact
    10381 tctttatcta atttcttaga taagtcttca gcaactggac gtaaagttaa ttttgcttta
    10441 tcactttctt cttttacttt acctaaatgt gaaaatagta cgattttacc accttgttcg
    10501 atgatgtatt gaattgtagg taaagcttga acgatacggt tgtcattagt aatttcaccg
    10561 tcttttaaag gtacgttaaa atcagcacgt actaggactg ttttaccttt aagatctaaa
    10621 tcagaaacaa tttttttagc catggtgttt cctccttgta attaggaaat tttatataaa
    10681 aataagcgga gaagcgtttg tgctccccgc ttactcataa gctttaatta atgctaaata
    10741 gttctgagca ctagcgtatt tgaataaaaa ctatactaaa attatttaga aagttcagct
    10801 aagtatgcta atgtacgaac taattgtgca gtatatgaca tttcgttatc ataccaagct
    10861 gcaactttaa ctaattgacg gtcaccaact gacattacac gagtttgtgt agcgtcgaat
    10921 aatgaaccgt aagtcatacc tacaacgtct gaagaaacga tttcgtcttc agtgtaaccg
    10981 aatgattcgt ttgaagcatt tttcatagct tcgttaactt gttcaactgt tacgtcttgt
    11041 ttttctaata ctactgttaa ttcagttaat gaacctgtag ctacaggaac acgttgtgca
    11101 ccaccatcta atttaccatc gatttcagga ataactttac cgatagcttt agcagcacct
    11161 gttgagttag ggatgatgtt ttctgctgct gcacgagcac gacgtttgtc accttttctg
    11221 tgaggtgcgt cttgtgtatt ttgatcacct gtgtaagcgt gaattgtagt cattaaacct
    11281 tcaactaaac caaagtcatc gtttaaaact ttagcaactg gtgctaatga gtttgtagta
    11341 catgaagcac ctgaaacaac tgtttcagaa ccatctaact cttggtggtt agtgttgaat
    11401 acgattgttt ttaagtcacc agtagctggt gctgagatta atactttttt agcgcctgct
    11461 tcaatatgag cttgtgcttt atctttatca gtgtagaaac cagtacattc taatactaca
    11521 tcgatattta agtctttcca aggtaatttg cttgcatctg gttcactgaa tgatttaact
    11581 tctttaccat ttacgcggaa accaccatca actacctcta cttcacctgt gaaacgacct
    11641 tgcatagtgt catattttaa taaatgcgct aacatgtcgt catctgttaa gtcgtttact
    11701 gctacaactt caagaccttc tacttcttga attcttctga atgctaaacg accaattcta
    11761 ccaaaaccat taattgctac ttttactgcc attataatgg cctcctttaa aatgatattt
    11821 aaaaagtatt aaacttttta tctcttattc aagtattatc tttgctgcgg cttcatcagt
    11881 gattaacact gtattcttgg gtgcaatcgt caagtatgct ttaattgctt cacctttcga
    11941 tttgcctcct gcaactgcaa aaataaagtc ttttgattca aggtcttcta attgaagtcc
    12001 aattgtttta accttatgga caatttgacc ttgtgtatca aaataataac caaatgcctc
    12061 tccgacagct tgatgatgtt gaagttgttc aatgaccttt tcaggtgatt gacgtcgatg
    12121 cgccatcttc agcgcatcac caatgccgtg taatataacg tttgcttgtt taattttgtc
    12181 taaagtgttt atgactgatg gctctaacaa caatgtgtta tatgttgttt cgctgacatt
    12241 atcaggtaca tacatcgtcg tataataacc gccagcttgt tgtgccatac tggctgcaat
    12301 tgtgtttgcc tgaaagacaa cattttcgcc tagtccacct ctggctggta cgaagaatac
    12361 attatatggt aataaatgaa ttgcttcact aacacatgcc atcgtggatc cgccagttac
    12421 agcaactatc gcatcttctt gtaatatgcc ttcaagtaat tgacctgctt gcctacctaa
    12481 ttctgtttta acagattggc tattatcagc atcaccagga acaacatgaa cttccttaat
    12541 ttgaaattta ttcttaatac cttctgacag acgattgtca tctgcataga tatcaaagta
    12601 atctttcaat tgcgaaatca gttgctcacc ttcagctgta atttccattc cggtaggctt
    12661 aacttttatc aaatcttgtt tcttaagcat atctgtttca gaacgcagta cacgttcagt
    12721 catatccata tgttcgctta aacttcgacg tccgacaggc tgattttttg agatagtagt
    12781 aagaatagaa aaacgtttat acattttatc tatgagatcc ggtataagct tttgctgtgc
    12841 ttgcaataag tctttcacta ctacctcctc cttatattta taaatgtaaa taaggatatg
    12901 tcacaaccag tggttgcctt tttaagtccc acgtgggaca aaaattaacc tctttacact
    12961 tgcaatagta ctattctatt tcaaaaattg caagcataaa acttaaattt tctaaataat
    13021 ttgataaagc gcttgcaata tattttcgat ttattataca acaaaacgtt ttattttatt
    13081 cgctgtagtc aaatgtatac ccttatactg attctctaat catttgctta ttatttgaga
    13141 ctaggacata aatcaatgct ctagaatctg aaaagttaca ttgccagtag ttgactgaaa
    13201 aaataaattt cactatctgc aaaataaaca actaactatt taagtatcat ctctccccta
    13261 attaacaatt ataaaaataa gaaccaatat tcgagacaac ctaattaatt gtgttgtctg
    13321 tcatactggt tcatttttgt ttgactaaat actacttatc attagcatta ttttgtttat
    13381 ttgttgttga ttggctattt tgttgtggtg cttgtggttt cgcttgttga tttgattgat
    13441 tgttttgttg attattattt ggctttggtg ttggttgttg cggctgtggt ttaggctgag
    13501 ctggttgttt attatcatca gttttcttct gattatcatc tgatttatct tgagatgctt
    13561 tgtcatcctt atctttcgac tcatcctttg atgctttatc atcattctct tctttagcag
    13621 gacgttgctt tgtaggtgct tgttgctgag gtggcacata ttgatattga ttctgctgtg
    13681 gcaattgctg atattggttt gcttgtgaat ctagctcatt ctgctctttt tcctcttgtt
    13741 gctttttctt tctctctttt tctgcatttt ctttttgacg cttttctttt tgttcctgct
    13801 tttgttcgtt caacatacgt tctttagctt tattcgaatg atctacatat gcaaaaattg
    13861 caaatactaa tcctccgagt aatagcaata tcgtcgcaat gctaactact ttcgctgttg
    13921 tactcatttt cttcttactt ccatcttcat ttttattgta gtcgtaattt tgattcgaca
    13981 tactctccct cacttcaaac taatttaaat atagaaatta tcacgtaact attaacattt
    14041 tcacaactaa tttgcttacc ttgtttctac aaacattaat aaacatttac tctttttgcc
    14101 ttcttcaact taaaactata ttcactatta ctcactcgta aatcggattc atttttacaa
    14161 agaaatagta gtcttatata tcttaacatt taataactaa atcaaacata ttttgagcct
    14221 tttttgaaaa ttaacatatt aacctttttg attttacaac aaattatagc tacgtattga
    14281 aaattaaagc attagtttaa gtgttgtgtt aaaagttttt atgtttagat tgttatatta
    14341 ctatctattc ttaaatttgt ttaaaaaatt aatgctttga attgatgtat aacgggaata
    14401 agatataaat aaaaacgacc cgcacgataa acgtacgggt ccactactaa agggagtcaa
    14461 atttttacct tcgtttgtat catgcagcgt tttacacata cttttaagag atgtttattc
    14521 gttatcgaag gtacaccttt attataactt atatcatttt tattaaaata caattcaaaa
    14581 aaagttattg acatattatt gtctaaataa tgtataacct ttgaacaaca ataatgatta
    14641 ttaaatagta actaaataca aaattacatg gggtgaatga taatgaaaca atacttaatt
    14701 actggtggga ctggtatggt tggatctcaa ttagttaatg aaatcaaaaa atcagattca
    14761 catatcacga tattaacgcg acacgaccaa atttcaaata ataagaaaat ttcatatgtc
    14821 aactgggcta aatctgggtg ggaacacaaa gttcctcaaa atatcgatgt ggtcatcaac
    14881 ttagcaggtg ctacattgaa taaacgatgg acaccggaat ataaacaaac gctaatgcta
    14941 agtagaattc aatctacgca agctttatat gaattgttta aatcacgtaa taaagcacct
    15001 aaagttttat ttaatgctag tgccactggt tactatcctc ctgatttatt tatgagttac
    15061 acagaagttt ataaaacttt accatttgat ttcttatcag atattgtgta tcaatgggaa
    15121 cgtttcgcac aacaatttga acaattaggt acacgagttg tcatcggtcg tttcggtata
    15181 attttatcga atgaaggcgg tgcgttacaa acaatgaaac taccatacga atattacatt
    15241 ggtggtaaat taggttctgg tcaacaatgg tattcatgga ttcatatcaa tgatttaatt
    15301 caagctattt tatttttaat aaataacgag tcagctagtg gtccgtttaa tttaactgca
    15361 cctatacctg agcgtcaaaa tttatttggc tacactttag caagagctat gcataagcct
    15421 catgaaactt gggtaccaag tcttgcaatg cgtctcatac ttggtcaaat gtcaacagta
    15481 gtattggata ctcaaaaagt attacctaat aaaattcaag cattgggatt ccaatttaaa
    15541 tatagtaatt taaaaatggc acttgaagat ttaattagta agtgagtggg acattgatct
    15601 ctcctactcc acttcaagca aaaagtctag gagataattc gttaaatctc tctagacttt
    15661 tctatttgct gatattacat atagatataa ctttaaagtc acttatgaat attggttgat
    15721 taattttaaa gtaaatacga aaaggccact tgaaacaaaa tgttcaaatg accttttact
    15781 actttataaa attaaatgta tgatttgtat tcaggatacc aaatttcagc ttcaattgct
    15841 tcatcaatat catcaatagt ttcagcgttt aatccttgat caatgacact ttgtgcaacc
    15901 gctttggcta tggcacgaga acattgatgt attttctcaa caggtggtaa gactgcagct
    15961 cctggttcat ctggattgac catgccacca agtgcatgac tcgcagctga tagaatctgt
    16021 tgatttactt tcttcgcttt tgacgcaata agacccaaac ctaaaccagg atacatcaat
    16081 gcattatttg cttgaccaat actgtaattc acaccattgt attcaatgtc attatatggg
    16141 ataccagttc caattaatgc ttttccatta gtccatttta ataaatcttg tgcagttgct
    16201 tctgctaatt ttgtaggatt agacaatggg aaaatcattg gacgttctgt atgttttaac
    16261 atctctttta ctgcatcttc tgtaaatgca cctgtttgag ttgatgtacc gattaaaaca
    16321 gtaggcttaa tattttcaat tacttctttt aaatttttcc agtttactgt atttgaaaat
    16381 tcattcctat ttttagcaaa tacttgttga gcttcagtta agtcatccat atcatcaaac
    16441 aacaatcctt gtttatccat aatatagaaa tgctgatttg ctttatcttc tgaaacatcc
    16501 tgtttaatta gttcatcttt taaaatattg gtaataccca taccagctgt accaccacca
    16561 taaactaaga acgtctgatt cgtatagtct actttagaaa tatttaatgc acctagtacg
    16621 ccggctaatg taactatacc tgtaccttga atatcatcat taaatgttgg taaagtatct
    16681 tcatatttat ctaaaatatt tttagcatgc cctctaccaa aatcttccca atgtaatagt
    16741 gcattaggat attctttacg cactgcactc acaaatttat ctacaaatgt gtaatatgtc
    16801 tcatcatcga cacgtttatg acgatttcct aaataaagtt catcattcag taattgttca
    16861 ttattagtac ctacatctaa agatacaggt aatacttgtg cgggatttat tccagctgcc
    16921 gctgtatata ctattaattt tcctatcgca atatctacac catcaacgcc ccaatcgccg
    16981 atacctaata taccttccgc atctgttact acgattaatc gaatatctct gccatttgca
    17041 acgttgcgca attcttgttc aatttggtct tgtctatcaa cagataaaaa tactgcatct
    17101 tggggtctac tgaaattctc attatattgt tcaatcgatt gtgcaataac agggtcataa
    17161 ataattggca taaattcaac taaatgctca gaagtcaatt tgtaaaacaa tgtacgatta
    17221 cgattaaaaa ttgccattaa aaataaacgt ttctcaaaat cggtttgttt cgccttgaat
    17281 tgttcataac attgttctgc ttgttgttct aaagttcgaa cattcgcagg taataatcct
    17341 tcaagcccta actgttttct ttcttcgtta gtaaaagctg tacctttgtt taaaaatgga
    17401 ttgttaagta attcaatgcc tttcataaaa tcatccttta tattttttag attgtagaac
    17461 cgcagacgta acgtgtctaa ttaaattctt ataaatattt ttacccgtta ttttttgctt
    17521 ttactcaaaa tattttgaac tatttattta taaattggtt taatcataca aaaagagcag
    17581 actgcttagt ctactcttta ctttgaatta ttttgtttca ggtaccatca cttcatcaat
    17641 taagccatat tctttagctt cttctgcagt taagaagtta tcacgatctg tgtctttttg
    17701 tattttttca atactttgac cagtacgctc tgataaaatg cgatttaatt tttcacgtgt
    17761 ttttaaaatg tgatttgcag caatttcaat ttcagttgct tgtccttgag caccacctaa
    17821 tggttggtga atcattactt ctgcatttgg taacgcaaaa cgtttacctt tagcaccagc
    17881 tgctaataag aatgatccca ttgatgcagc cataccgata caaattgttt gaacatcagg
    17941 tttaatgtgt tgaattgtat cataaatcgc aaaaccagct gttacacttc caccgggtga
    18001 attaatgtat aaataaatat ctttctctga gtcttgtgct tgtaagaata ataactgtga
    18061 tacgattgaa tttgctacgt tgtcatcaat ttgtgaacct aacataataa tacggtcttt
    18121 taataaacgt gagtatatat cgtatgcacg ttcaccgcga tttgttgttt caataactgt
    18181 aggaattaaa ttcatctata tttcctcctt gtaataactg ttactcatta ttttacatat
    18241 taagtcaaat ttggtcaaac actttgactt taaatagtta cattgtttga cgcttgtctc
    18301 tttcctttgt aaactgataa tatctatttt agattgtact tattttactt ctcatagtac
    18361 agtgatttga ttaacacatt tactccttgt ggtgtaatgg ataacacgta agattccggt
    18421 tcttaagata ggggttcgat tcccttcaag gaggcatttt aataaaatac aatacttttc
    18481 aatacagaga atcccgcaat gtcgggattc tttttatgtt gattttttgt ctatgatcag
    18541 ggactttcag ggactcaatt aattatcaca ttcatatttt cttatgacca aattgatata
    18601 tatgatgcta cttaatgaga aaattaggta acgcttggtt attgaatttc cttcattaaa
    18661 cttcccagct taatttaatg gttagtcgac tattattcat taaacacttt ttaatcataa
    18721 aaaaggtatt tttgataatt caataccaaa aacaccttct tacttataat tctatttgtt
    18781 caccatttct aatcttatcg gctaaatcat taagttttct taatcgatga tttacacctg
    18841 attttgaaat tggaccagtt gataccattt ctccaagctc tttcaacgaa atttcttgat
    18901 gttctactcg aattctagca atctctctca acctgtctgg taaattttca ataccaattt
    18961 ctttatcaat caatttaatg ctctcaactt gtttcatcgc agcactaact gttttattta
    19021 gatttgccgt ttcacaatta acgagtcggt taacagaatt acgcatatct cttacaatac
    19081 gtacgtcttc aaattttaat aacgcttgat agccacctat caaactaaga aaatccgaaa
    19141 tcttttccgc ttcttttaaa tacgtaatac ttcctttttt tcgctctaaa tgtttggcat
    19201 tcaactcata actattcatt agtttcgtta agccttctgc atgactctca ttttgagaaa
    19261 aaatttccaa atggtatgaa gatgtttcag ggttattcac tgagccacct gccagaaaag
    19321 ctcctctcaa gtaactacgt ctcatttcgt catcttgaat cattgaatga tcaatttcat
    19381 gcgtaaaaat gccgtctttt aaaattccta attcatcaag aatttctttc gctttcatct
    19441 ttgtacgaca aatataaata ttattttttt taagtttcat ttttttacgg actaatattt
    19501 caacttccac attaaagaca cgtttaatca acgaataaat acgtcttgcc gttgttgcat
    19561 tttccgtttg aacatttata acaaattgtt gatttgaaag actaagtgca ccattcattc
    19621 gaatcagtgc actgagctct gcttttgcat tcatttcatc gacgtctatt ctagttaatt
    19681 catttttcat ttctgatgca aagctcatcg tacagtcatt cctttcttat ttaaaacatg
    19741 attcacctta gaaccactgt ctattttcat ttttatacag ctctattatc atatcataat
    19801 atgattacgt tctatattat ttacgtttat cacttggtac gaaaggaata gtactaatta
    19861 attctaaagc tatgtcataa atcattgtcg ataacacttt agtattatgt cttactaaat
    19921 gattttcaga aatttcaact aaattagaag atgtttttac atttatgctt tctttttcta
    19981 gttcagcctt attaacttca actggtttag aatgtttttc ttcatatttt ttcaaaactt
    20041 gagcattgaa agtttgtgta ctacaaatga cataatcaat aaacggttgt ccggcttgtc
    20101 tatgaatcgc gtcgatatga tctttcacgc tataaccatc tgtttcccca ggttgcgtca
    20161 tcacattaga tacatatagc ttaggcgcat cagaatgaat taatgcatct gaaataccat
    20221 tgacacataa gttagaaata acgctcgtat ataatgaccc tggtccaaga acgattaaat
    20281 ctgcttccct taaagcatcg attgcttctt ccattggttg cacatcgtta ggttctaaaa
    20341 acacacgatc aattttttta tgttttttag gaatatttgt ttctccaaaa acaatttctc
    20401 catcttccat aacagcattt aattgcacac ttgtatttgt agatggaatg actctacctt
    20461 taatatttaa aattttactt aatgctttaa tggcatgtcc gaaatcattc gtaatattag
    20521 tcatacctgc gattaataaa ttacctaatg agtgaccgct aatttgattt tcttcaaaac
    20581 gatactgaaa aagttggctt aaaactgact cagaatcact taaagctgca atcacatttc
    20641 tgatgtctcc tggtgctggt atatccattt catctctgat tttccctgta ctcccaccat
    20701 tatcagcaac tgttacaatc gctgtaatat caatcgggaa ttctcttaat cccctagcca
    20761 taactgataa gccagtgcca ccaccgataa gtacaacttt tatttgtctc attttttctc
    20821 gccactttca atatgtgcgt ccctatgatg cacataaaca ttatattcaa atacttcatt
    20881 tagataatta cctagtcgtt ctgctaatgc tacagatcta tgttgtccac ccgtacaacc
    20941 gatggcaatt actaattgag atttcccttc ttttttatac ccaggtatca taaaatctaa
    21001 caaatcagtt aatttttcaa agaaaatctc tgtctctttc catttcataa cataattata
    21061 aacgtcttta tctaatcctg ttaaaggtct taaatctact acataatatg gatttggtaa
    21121 aaatcgtaca tcaaatacta aatctgcatc catctgaatc ccatgtttaa aaccgaaact
    21181 tgtgacatta attgtaaaag tttcaaactc ttcatcttca tagtatcgac gaatgcgttc
    21241 ttttaattct ttaggtgata actttgtagt atctataaca aaattagcta tacttctaat
    21301 ttgagacaaa tgctctcgct catcattaat tgcattgatt aacgattttt taccttgttc
    21361 catcaaagga tgtgcacgac gcgtttcctt atatcttgaa attaattttt cagtactagc
    21421 ttctaaaaac ataacatcaa tgatgacgtc actttcactt ttgactttat ccactactgc
    21481 aactaatgaa ttaaatagtt ccttacctct taaatcaatt gcaattgcca cttttcttaa
    21541 ggatggattt ccttgttcca tcaactctac aaatttaggc aataacactg gtggtagatt
    21601 atctacacaa aaatatccca tgtcttctaa acattgaata accaaagatt tacctgcgcc
    21661 agataaacct gtgacaacta ataattcact tttacttttt tctttttcat tattatccat
    21721 tattttttca ccgccaaaac gaatttcatg atgttaattt aaatgttcta tgacaaaatt
    21781 aagcaacgat gttttatatt tattttaaac tatgtctaga ttaaatttct aaaaatatac
    21841 atcatttaaa aggagattgg gacataaatc aatgtcctag ggtctataat attatattgc
    21901 tagtagttga ctgaatgaaa atgcgcttgc aacaagcttt tttcaattat agtcaggggc
    21961 cccaacaaag aagctgacgt aaagtcagct tacaataatg tgcaagttgg ggtggggccc
    22021 caacacagag aatttcgaaa agaaattcta caggcaatgc gagttggggg gtgggcccca
    22081 acacagaagc tgactttccg ccagcttaca ataatgtgca agttggggcg ggac
//
LOCUS       DAKSQE010000036        21755 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.036, whole genome shotgun sequence.
ACCESSION   DAKSQE010000036 DAKSQE010000000
VERSION     DAKSQE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21755
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.036"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(97..1329)
                     /locus_tag="O0V33_001190"
     CDS             complement(97..1329)
                     /locus_tag="O0V33_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HCX8975835.1"
                     /translation="MGRNLKLKHRESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQT
                     AVFTSQFLASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISIL
                     IAFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMI
                     LGIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAH
                     RILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVF
                     EHAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLKERSNLIVIVFIVIST
                     MIFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAV
                     LVTILTGIFSFKEVLQLF"
     gene            complement(1341..2093)
                     /gene="pxpA"
                     /locus_tag="O0V33_001191"
     CDS             complement(1341..2093)
                     /gene="pxpA"
                     /locus_tag="O0V33_001191"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCX8975836.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNEEIYNLMIYQLGALQGFCRIHQ
                     VKINHVKPHGALYQMGAKDREIASVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            complement(2093..3454)
                     /locus_tag="O0V33_001192"
     CDS             complement(2093..3454)
                     /locus_tag="O0V33_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCX8975837.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALDSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNDAVNHVSEIEKLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NYVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDNAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQNDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLRATLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            complement(3468..3917)
                     /locus_tag="O0V33_001193"
     CDS             complement(3468..3917)
                     /locus_tag="O0V33_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HCX8975838.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKVNDNSDVSTNDYHDIKSPMVGTFFLQDSKELTEPIVKVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            complement(3919..4929)
                     /locus_tag="O0V33_001194"
     CDS             complement(3919..4929)
                     /locus_tag="O0V33_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCX8975839.1"
                     /translation="MSIKILQPGLFSTVQDLGRKGYEHIGFSGAGAMDQFSFKVAQSL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSVNASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHTINVPPIAESYATHTRSSIGGFKGRKLLANDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLATILKG
                     DL"
     gene            complement(4919..5653)
                     /gene="pxpB"
                     /locus_tag="O0V33_001195"
     CDS             complement(4919..5653)
                     /gene="pxpB"
                     /locus_tag="O0V33_001195"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCX8975840.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITPSKLIENLELNKFNEKNVHAVNQTNSIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     EFIQIERDISDGNFNVDDWVVIENVN"
     gene            complement(5979..6455)
                     /gene="greA"
                     /locus_tag="O0V33_001196"
     CDS             complement(5979..6455)
                     /gene="greA"
                     /locus_tag="O0V33_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCX8975841.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            complement(6483..7106)
                     /gene="udk"
                     /locus_tag="O0V33_001197"
     CDS             complement(6483..7106)
                     /gene="udk"
                     /locus_tag="O0V33_001197"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCX8975842.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            complement(7106..8374)
                     /locus_tag="O0V33_001198"
     CDS             complement(7106..8374)
                     /locus_tag="O0V33_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCX8975843.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            complement(8386..9309)
                     /locus_tag="O0V33_001199"
     CDS             complement(8386..9309)
                     /locus_tag="O0V33_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCX8975844.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLMDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            complement(9312..9950)
                     /locus_tag="O0V33_001200"
     CDS             complement(9312..9950)
                     /locus_tag="O0V33_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCX8975845.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLVTHHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            complement(10235..10543)
                     /locus_tag="O0V33_001201"
     CDS             complement(10235..10543)
                     /locus_tag="O0V33_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCX8975846.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            complement(10558..10986)
                     /gene="ruvX"
                     /locus_tag="O0V33_001202"
     CDS             complement(10558..10986)
                     /gene="ruvX"
                     /locus_tag="O0V33_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCX8975847.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            complement(10990..11250)
                     /locus_tag="O0V33_001203"
     CDS             complement(10990..11250)
                     /locus_tag="O0V33_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCX8975848.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            complement(11313..13943)
                     /gene="alaS"
                     /locus_tag="O0V33_001204"
     CDS             complement(11313..13943)
                     /gene="alaS"
                     /locus_tag="O0V33_001204"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCX8975849.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAVSGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIVSAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDNQVVDKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     gene            complement(14286..16763)
                     /locus_tag="O0V33_001205"
     CDS             complement(14286..16763)
                     /locus_tag="O0V33_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCX8975850.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDSEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEDMLENTATNDTGASQTTIDDQVEAPMSSNASEEVTSDSTKTDTN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            complement(16765..17433)
                     /locus_tag="O0V33_001206"
     CDS             complement(16765..17433)
                     /locus_tag="O0V33_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCX8975851.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEEDPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFYNLANVYYNQQRYQESIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNENIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
     gene            complement(18115..19233)
                     /gene="mnmA"
                     /locus_tag="O0V33_001207"
     CDS             complement(18115..19233)
                     /gene="mnmA"
                     /locus_tag="O0V33_001207"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCX8975852.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            complement(19234..20376)
                     /locus_tag="O0V33_001208"
     CDS             complement(19234..20376)
                     /locus_tag="O0V33_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCX8975853.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMTIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVETMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            20686..21699
                     /locus_tag="O0V33_001209"
     CDS             20686..21699
                     /locus_tag="O0V33_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCX8975854.1"
                     /translation="MAIKYSALNLVPIREGEDERTAINDMVKLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSEHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHELQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQLNEK
                     NTL"
BASE COUNT         6629 a         4281 c         3039 g         7806 t
ORIGIN      
        1 tctaattatg ctgtataaat gaatcaccga taatcaaaac acatttcggc ctaatactaa
       61 caacatttac agtgatgcta ttagtcgaaa tgtcttttaa aataactgaa gcacttcttt
      121 gaatgagaat atacctgtca atattgtaac aagtactgca acgataccaa agatgaacat
      181 ccaatttggg tgtttataat cacctacaat tgatttcttt ttacttgcaa tcaaaattgc
      241 acctaacgta ataggtaaaa tccatccatt tatcgcgcct gctataatta aaaggctgat
      301 tggttttcca ataaataaga aaatcattgt tgaaataacg ataaacacaa tcacaattaa
      361 attacttctt tctttaagtg atttatgaag tgtttttaaa aatgttgcgc ttgtgtatgc
      421 tgagccaatt actgatgaca tagctgcagc aaataacaca ataccaaaaa tattctttcc
      481 aattggtcca attgcgtgtt caaaaactga cgctggtgga ttttcagaac ttagtgtcac
      541 acctgttaca acaactccta ataccgctag gaaaagtaac gttctcataa tacctgtagt
      601 taaaatacct gcaattgctg attgatttac aaatggtaaa tattgcttac ctttaatgcc
      661 agagtctaat atacgatgtg cacctgcaaa ggtaatataa ccacctacag ttccaccaac
      721 taacgtaatg attggcaaga ctaatttcat tggatgttct ggcgcaaatg tatgcacaaa
      781 agcatcacca taaggtggat tagaaacaaa catcacatat gccacaacta aaatcatcac
      841 aataccaaga atcattgaaa caacgtccat aattttttgt ccacttttac ttacaaagat
      901 taatattgca aagattgcag taatagctgc gccccatttt acatctaatc caaaaattgc
      961 atttaaacct aaaccggcac cagcaatatt accaatatta aaagctagac caccaaatgc
     1021 aatcaatatt gagataacag taccaagccc aggaacaact ttatttgata tttcttgacc
     1081 tcttaaacca gttacaacta atatgcgcca tatattaatt tgtgcaccaa tgtcaatgat
     1141 aatagacagt aatatggcaa atgcgaaact cgccaaaaat tgcgatgtaa atactgccgt
     1201 ttgtgttaaa aatgctggtc ctattgcaga ggttgccatt agaaatacag aacctaacaa
     1261 taatctctta tggtttttag taaattcaaa atcactttcg cgatgtttaa gctttaaatt
     1321 tctccccatt ttttagcccc ctataaggat tgaatatcaa tgccttcttt cattaaaatt
     1381 tctctaattt tcgaaacaaa taataatgca tgttctccat caccatgcac acaaattgta
     1441 tctgcttgta acgttacttc cttattgttt tttgaaataa ctttattttc cttcaccatc
     1501 ttcaaaacct gcttaagtgc ttcgtcagta tcagtaatca cagcatcact ttcttttcta
     1561 ctaacgagct gcccatcatc ttcgtatcgt ctatcagcaa acacttcaga agctgtaatt
     1621 aatccgacat tctttgcttc tgaaattaga tatgaatttg ctaatcctac taacactagt
     1681 gatggatcaa agtcataaac agcttgtgct ataacgcttg ctatttctct gtcttttgca
     1741 cccatctgat acaatgcacc atgtggttta acatgattaa ttttaacttg atgaatgcga
     1801 caaaaccctt gtaatgcacc taattgataa atcatcaaat tataaatctc ctcgttagag
     1861 atatctatat ttcgtctgcc aaagcctttc aaatcaggta aaccaggatg tgcacctact
     1921 gcaacattat gtgctttggc aagttttacc gtttcattca ttacattttc atcaccagcg
     1981 tgaaaaccac aagcaacatt cgcacttgta attaacggaa taatttgatg atcaccacca
     2041 aaggaataat ttccaaatgc ttcgcctaaa tcacaattca aatcaactcg cattataatt
     2101 ccaccccttt aacaatttga tgtttttcta aaaatttaat atcaacatct tttgcatctc
     2161 catcacgata tagtggataa tttaaaactg catataaaaa atcggcagtt gtagaaaatc
     2221 catctatcac catttcatct aaggtagctc tcaatttatc aattgctgaa gctctatcat
     2281 gagattttac aattacttta gctactaaag aatcataata tggtgaaact tgataaccgt
     2341 gatatagtaa agaatcgact cgcacattaa agccttgagg taaatgtaac gctgtcactt
     2401 tacctggtgt tggttgaaat ttcttttcag gattttcggc atttattctc gcttctatca
     2461 catgaccatt aaattgaata tcgttttgtg aaaaaggtaa atgattatgt tccaataaat
     2521 acaattgtgc tgcaaccaaa tcacgttctg ctcgcatctc tgtaacagta tgttcaactt
     2581 gtattcgagc attcatttca ataaagtaat gtgcgttatc agttactaaa aattcaatcg
     2641 tacctgcact tctataattt gctgcacgtg caactttaac agcatcgtta catatttgtt
     2701 gtcgtctttc ttcagttaat gctgcacaag gagattcttc gattaatttt tgatttttac
     2761 gttgtacaga acaatcacgt tcccctaaat gtacataatt atcctgccca tcccccataa
     2821 cttgaacttc aacatgtttt gcaacaggta taaaagcctc aacataaaca cgatcatcat
     2881 caaagtattt ttttccttca cttttagctt ctttaaatgc cttttctaaa tcttcagctt
     2941 tctttacaat acgtatacct ttaccaccac cgccactggc agctttgata acaactggat
     3001 aaccgatgtc tttggcaagc ttctcaattt cagacacatg attcacagca tcatttgatc
     3061 ctggaatcac aggaacacct gcatgatgaa ctgtttgtct tgctgttatt ttatccccca
     3121 tcatttccat cgttttttta gtaggcccta taaacgctat gccttgttcc tcaacggttt
     3181 gagcaaattt tgttgattct gataaaaagc catatcctgg gtgaattgca ttagcaccag
     3241 tgatttgtgc agcagatatg atgcggtcaa tatttaaata actatctaaa gcattagctt
     3301 ctccaataca tatagcttga tccgctaaat gtacatgcaa gctttgctcg tccccttttg
     3361 cataaactgc tacagtttca atcccatatt ctctgcaagc tcttataatc cttacagcaa
     3421 tttcacctct gttcgcaatt aaacaacgaa gcatttactt acccccttta cttaatacgt
     3481 accaaaactt ggtcgtattc aacatttgtg ccatgatcag ctactatttc agtgatttct
     3541 ccagcaacat ctgttgttac ctcgtttaat actttcatcg cttcaacata tcctataata
     3601 tctcccttgt taactttgtc accgactttc acaattggtt cagttaattc tttactgtct
     3661 tgtaaaaaga atgtacctac cattggtgat ttaatgtcat gataatcatt tgtcgaaaca
     3721 tcggagttat cattcacttt tgaagctgtc aaatcattat tgttcatact ttgatttgat
     3781 tgattactgt gtgcagccaa atgattcgaa tcagtgaagt caatttctat ttcatcttca
     3841 aaatttttat atttaaattt cttaacatca ttttccttca ctaatttgat tatttgttcg
     3901 attttttcaa tattcatttt acaaatcccc ttttaaaatt gttgctaatt ttttcgaagt
     3961 atgtcgcaag ctagatgtat caaaaattgg agtcttttga tgactcttaa gaatttcatt
     4021 aaacagagac atttgttccc gattcttatc tacagcttct tggaatgata tccatttaaa
     4081 ttgaattgta tcttgtggtt tcatctgtgc taactttggc agatcaaatt tgcatacagt
     4141 tgcaattttg gtataaccac ctatcgtttg tttatcatta agaagaataa taggttgacc
     4201 atcatttggt acttgaacac tgccaagagc aaccggttca gaaatgatat ctgcttgatt
     4261 aataggtgca acgctgtcac cttccaaacg ataccccata cggtctgatt gttcggtaat
     4321 taaatatgga tgatttacaa ttttcgctct agcctcttca gaaaatgcct cgaattgagg
     4381 tccttgaaga atgtgtataa tattattttc tggcaataaa tcgtcctgta aatgaatagt
     4441 ctttccaatg ttttctttaa agtcattatt tattttcact gttattacat cattagctaa
     4501 taactttcta cctttgaatc ctcctatact acttcgggta tgtgttgcat aactttcagc
     4561 aattggaggt acgttgatag tatgaccaaa agtaagataa ccgcgtgcac ctttggttat
     4621 agcacctatt tttaaaatgt cacctttctc agctaatatg acagaattca ttgatatagt
     4681 tttattattt agcgaggcat taacactacc accggttata acaaatgtat tttgcgtatt
     4741 aaattgaatg gtaggaccaa tcaaagtata ttcaatcgct ggaccatcat tgttaattaa
     4801 tgactgcgca accttaaaac taaattgatc catggcacct gcgcctgaaa acccaatatg
     4861 ttcataacct tttcttccta gatcttgtac cgttgaaaag agacctggtt gtaaaatctt
     4921 aattgacatt ttcgatcacc acccagtcat caacattaaa gttgccatct gatatatctc
     4981 tttcgatttg tataaattcc tgttcatcta ttgcataaaa ttgtatccat tctcctgctt
     5041 catacattga cattggttca cgctcgctgc taaatacttt taacggtgtg cgtccaataa
     5101 tttgccatcc gccaggagaa tctgatggat atagtcctgt ttgattattc gcaataccta
     5161 cagaacctgc atgaattttt aaccttggct gattacgtct aggtgtatgt agttgttcat
     5221 caagtccgcc taagtatgga aatccaggca taaatcctag catatatatt aaataaggtt
     5281 tacttgtatg tttttcaata acttgctcaa cagttattcg attatgcttt gctacttctt
     5341 caatatcagg tccatatgta ccaccatatt gaacaggtat tttaataata ctattggttt
     5401 gattcacagc atgaacattt ttttcattaa atttgttaag ttctaaattt tcaattaatt
     5461 tagaaggtgt tatagcttgt tcatcaaaat atattagaac tgctcgatac gaagggacaa
     5521 tatcttgaat ttctaatatt tctttttctc gtatccaccg taccattgct gtgacatttc
     5581 gatatgtctc ttcggatatt ttattttcaa aataaatcat aatcgtctgc tcgttaataa
     5641 atcttacatc cactctaaat ccccctttgt attgcaataa accagtattg aatacctttt
     5701 cattgtatca ttgagaagca caagttgttt aataagtaat tcaaatcgca tacaataaca
     5761 aattataatc aatttgtttc ttcggaaata ttttgctttt ttaaaatgga gtagttaata
     5821 ttgaaactct tacactaatt tagattctgc tatcaatcgg tctgatggaa aattttcact
     5881 tttcatctgt ccgatttttt tattttgaat ataaaaaagc acgaccgaag tatcattaac
     5941 acacttcaat cgcgcaatta aacaatctat ttgatcattt attggatatt aacaattttt
     6001 acgttcattt cgccaccatt aggtagtgga acacgcactt catcatctaa acctttacca
     6061 attaacgctt tagccattgg tgattcattt gaaatcttac cattaaatgc atctgattca
     6121 gctgaaccaa cgatttgata actttcctct tcatcacctg gtaattctac aaacgttact
     6181 gttttaccaa ttttaacaac gttgttatct ccagtatctt caatgattaa tgcatttctt
     6241 aacatatgct caattctttg aatatcttgt tcgatgaatc cttgttcatc ttttgctgca
     6301 tcatactcag agttctctga taagtcacca aatgaacgtg caactttaat tttctctaca
     6361 acttcaggac gcttaactgt ttttaattct tcaagttcac gctctaattt ttcaaaacct
     6421 tcttgagtca ttggatattg cttttgattt tccatattgt catcttcctt tactgaatta
     6481 tactattgct tgctaactaa agactgaatt tttgttgtca taatatctat tgcaacttta
     6541 ttgctcccac cttcaggaat aattatatca gcatatttct tagtcggttc aataaattgg
     6601 tcatgcatag gtctaacaac acttaaatat tgattgataa cagagtccat tgaacgccca
     6661 cgctctttag tatctcgtgt taaacggcgt aatattctca agtctgcatc tgtatcaaca
     6721 tatattttaa catccatcat atcacgtaat accttatttt ctaaagcgaa aataccttct
     6781 acgataataa catctttagg tttaaaatca atggtaatgt cacttcttgt atgactagca
     6841 taatcatatg tcggtacttc tactgcttta ccatttttca agtctttaag attttcaatt
     6901 aataaatcat tatcgaatgc aaatggatgg tcataattgg tttctaggcg ctcgtcgaaa
     6961 gtcaagtgct tttgatcttt ataatagtaa tcttgagcaa gtaaagcgac actatgacct
     7021 tctaagtttt tcataatttc gttagttaca gttgtttttc ctgagccaga tccaccagct
     7081 atgccaatga ttgtagtagc tttcattagc caatttcctt tctcatcatg ttgtttggat
     7141 atatcgggcg atccacttta atttgaacga tttgtaatgg atggcgcgcc gcgtctaagc
     7201 tgttaccttc ttcatcataa attgcttcta ctacttgtgt aaatgtttca atttctggac
     7261 caaagaattc tatttcttga cctggtttaa agttatttcg ttgttgaata gtcgcaattt
     7321 ttgtatcttc attataatct aataccaaac cacaaaaatc aaatggtgat tttttagatt
     7381 gttgttgacc aaacatctgt tcttcataac caggtgttcc ctcaaagaat gcaggtgcag
     7441 tgtctctatt tgcacattta tctaactcaa ttaaccattc cggattaatc ttaaagttgt
     7501 caggatctgc cgcatacgca tcaatgactt tacgatatac tgagacaact gttgcaatat
     7561 agtgaattga cttcatacgt ccttcaattt ttaatgagtc cacaccaata tccatcattt
     7621 gaggaataga ttcgattaat tttaaatctt taggactcat cgcaaatggt gtaacttcac
     7681 cttgattata aaatacatca agttcaccat tatcatcaac ttctaataat tcataatccc
     7741 aacgacaact ttgacagcaa ccgcctctgt tggaatccct tgcagtcata tgattactta
     7801 atgtacatct acctgaatag gcgatacaca tagcaccatg aataaatgct tcgatttcaa
     7861 tatctacttt ttccttcatt tcacgcattt ccatcgcgcc ggtctcacgt gctaatacaa
     7921 cacgatccaa tccttcttct ttccaatatt ctacagcttt gtaattagaa agtgattgtt
     7981 gagtagataa atgaatttca agttttggcg caacttcttt acatgtttcg ataattaaag
     8041 gatctgcaac aatgatacct gtcgcaccag tcttttccaa attacgcaaa tatgattcta
     8101 gaccttcaat attctcatca tgtgcaataa tatttgtcgt aacataaatt ttggcaccgt
     8161 aacggttcgc aaattcaaca ccttcagcta tttcttccat cgtgaaatta tcagcatttg
     8221 aacgtaatcc atattcttga ccacctaaaa atacggcatc agcgccataa tgtactgcta
     8281 tttttaactt ttctaagttt ccagcaggtg ctaataattc tggtttcttc ataactgttt
     8341 taggagttga tttaatctct tctattgtct tcatgattaa cctccttaat atactgtttg
     8401 tttatataag aaaccttcgt caaatggtcg atgatcaggt tgaatttctt caattgggtc
     8461 catcaacata aatttctcat cttcatagat ttcaggatct tcattgtaca aatctatcgc
     8521 ttgacgatac tgttccgtta ccacattaat atattcttcc gtttgtagaa taccatcgat
     8581 tttaaatgaa tctatacccg cctcaaaaaa tggtgctaat tcttcaatta aacaaatgtc
     8641 gtttggtgac ataatgtgcg taccattgta atcttcgtaa actgggtaat tattttgtct
     8701 ttcttcatca taaagcaata aagattgttc atcattgcga cgttcaattt tcatttgacg
     8761 atcttggaac gtataataat tgcctagtag catacgcttt gattggaaca tacaagtcat
     8821 tccttgaact tgcacctcaa tttccacatt tgaattttct tttatattaa taatttcatc
     8881 caaattcagc tcacgtgcta agacagctct tgatgcgcct cttttacccc agtaattaca
     8941 ttgaaaatga ttagttacta acgtctctgc attccaatga agtggtattg gattttcttg
     9001 cgccttcaca tacattacta ctgctggatc cccgaaaata attctgtcaa ctcgtatttc
     9061 atgtaaaaaa ttaatataat cttctacagc atctaaatga taattatgaa ataatccatt
     9121 caccgccgca tatacttttt tatcgttttt gtgagctaat gcgacagcct ctgtcatttg
     9181 ttgtctattg aattcccctg gaagtcttaa accaaacttt tgctcgccaa ttacaaaagc
     9241 atctgcacct aaatcaataa gtgtttccat atggcttaat gacttgggtg tgacaagtaa
     9301 ttctgtcata gtcattctcc tttaattgaa atcgctaatc catcgtctat atttaaaaaa
     9361 ttcgttgtat atcctggttg ctttattaac cactcattat aatcttgaac ctttttaacc
     9421 atttgtctta catttctcga tctaacaatc ccaatatccg atacaaaacc gtgatataaa
     9481 acattatctg taattacgag accttggtgc tttaaaagtg gtgtatatat ttcaaaaaat
     9541 ttctttgatt gcgcttttgc tgcatcaata aatatcatat cataaacttt gtcatttaca
     9601 ttttcaaatt gctctaaagc attaccttca ataattcgaa cctggttttc aaaatgatga
     9661 gtaactaaat tttgtttagc atattgaatc atcgtttcat tacgctctat cgttgtgaca
     9721 tgaatgtcat cagatataga agcgaattgc atagaactat agccgattgc tgtaccaatt
     9781 tctaaaatat ttttaacatt attcatacga attaattgct taattaaatc taatgttaaa
     9841 cgatccacga ttggcacttc atttacttcg gcaaattcac gcaaaacttc gattgaacta
     9901 ttttgatgtt gatgtaaatc tattaaatat tttttattta ggtcatccat gttttaaact
     9961 tcctttatgt aaaataagtc aatatgatta tgacaataaa ataaatcagc cttcacaatt
    10021 gattataatt ttgccaacca attaaatgac tgatttcgtg ttagacgcaa agctatttta
    10081 tttatagaag cgaatcattc atataaaatt taactttaga tattttacca tattttcaat
    10141 aaaattataa gcgttaatta tttatacatt gtttgactta aaaaaatact cttgcctccc
    10201 catcttaagg ttagcaagag taaaatcttt ttaattattc ttccatttca gtatttacaa
    10261 cttcttcaat catgtcccat tcttcatcag tttcgattgg tactaactta ccaccgtcac
    10321 ctgactcatc tggttcattg atcattggta caagctcaat catatcgtct tcatctgatt
    10381 gagcaccttc ttcagctaag ataacatact cttttttgaa ttcaggatga taaaattcta
    10441 aaacttttcg gtataaaact tcatttccct cttcatcgaa taaagttaat aattcttctt
    10501 cgttattaat ttctagttgt gaatcatgat tatgttcagt catagtaaaa tctcctttta
    10561 atgtagtgaa tctaaatagc cttgtaaaat aaataccgct gccattttat caatcacttg
    10621 ttttcttttt tgtcttgaaa catctgcttc taataatgat cgttcagcag ccattgtgct
    10681 taatctttca tcccacatca caatctcaat agaaggataa gcttctaata atttttcttt
    10741 atatgttaac gaagcttcgc ctcgaaatcc tattgaatta ttcatgtttt taggtagtcc
    10801 tattacgact gtacccacat tatgtttttt aataatgtct actaattggt caatacctaa
    10861 ttcattattt tcttcattga ttcggagtgt gtctaatcct tgtgccgtcc aacccattat
    10921 atcactaatt gcaattccta ccgttctact accgacatcg agtcctaaaa ttttatgttg
    10981 taacataaat tatttatttt gctcttttaa atagtaagaa acaagctctt ccataataac
    11041 atctctatca atatgacgaa tttgatttct tgcttcattt tggcgtggaa tatacgcagg
    11101 gtcacctgat aataaataac ctacaatttg gtttacggca ttatatcctc gttcatctaa
    11161 tgttcgataa acattattta aaacatctct tacatcttgt gttggaagtt cttcatagtc
    11221 gaatttcatt gttttatcaa agttttccat ttgcgacact cctttaatta caaatataac
    11281 tcactatcat catacaatat tatggcttta aattatagat ttttaatgta atctttaata
    11341 aagcttaatg attttgagat attttcaggt tgtgtaccgc caccttgagc catatctgga
    11401 cgaccgccac ctttaccacc aacgattggt gccatttgtt tgataagatc accggcttta
    11461 acattatttg ttaaagattt agggacagtt gcaaccatcg atactttatc atcaacatta
    11521 cttgcaagaa tgataattgt atcttgtagt ttagatttaa aatcgtccat tgtcgagcga
    11581 attgctttcg catttggtac atccacttca gtaaccaata ctttatagcc attgatttct
    11641 tcaacttgat cttcaatatt acccatttta agtgatgtga tttctttgtc acgttgctct
    11701 aattgtttta ataatgcttt ttcttcatct tgcaattgtg ttaacttatc gactacttga
    11761 ttatcagatt tcactttcag ctgtgatttc atcgtattaa atttctcttg aatatcttct
    11821 aaatataaga aagctgcttt acctgttaat gcttcaatac gacgcacacc agctcctgta
    11881 cctgactcac ttactatttt gaataagcca atttcagaag tattgcggac atgaatacca
    11941 ccacataatt caattgaaaa tggtgccata tttactacac gcacaacatc accatatttt
    12001 tcaccgaata atgccattgc gcccatttct ttagctgaaa caatatccat ttcttgaatg
    12061 ttaacgtcaa tacctttcca aatttcttca tttactaagc gttcaacttg atcaatttca
    12121 tcattagtca ttggaccaaa atgagagaaa tcaaaacgta aacgatctgc ttctactaat
    12181 gaaccagctt ggttaacatg atcacccagt actgatttca acgctgcatg taataaatgt
    12241 gttgcactat ggttcttttg aatgtcacgt cgatcatttt ggttcacttc agcagacact
    12301 gtagcgccaa catttacttg gccaaattgt actactcctt tatgcaagtt ttgaccattt
    12361 ggcgctttgg ttacttcact aacagcaatt tcaaaattgt cattatatac aatacctgta
    12421 tccgcaactt gtccaccact gactgcataa aatggtgttt ccgttaacat gaagtatact
    12481 gtttcacccg cttcaacttg tgaaacttct tcaccattat atatcaagtg tgttagtgtc
    12541 gtttgagctg tcgcagtatc ataaccaaca aaagtacttg cagatgtaat atttttcaat
    12601 acttcacttt gaacttgcat tgattgagaa ttttgacgtg cttgacgtgc acgatcacgt
    12661 tgttgttgca tttctgactc gaatgttgtc atatcaactt tcaatcctgc ttgcactgct
    12721 atttcttcag ttaattcaat tgggaacccg tacgtatcat acaatttaaa tgcatctttc
    12781 ccattaattt catttgttgt cgctttagct tttttaatta attcatttaa aatcgctaaa
    12841 ccatcttcta atgtttcatg gaatcgttct tcttcagact ttataacacg cttaatgaaa
    12901 tctgcttttt ccttaacatt tggataatat ggttccataa tgtctgcaac aatatcaaca
    12961 agtttgtaca taaatggctc attgattcct aacgtttgac taaaacgaac ggcacgacgt
    13021 aacaatcgac gtaatacata tcctctacct tcattggcag gtaatgcacc atcagaaatt
    13081 gcaaatgcaa tcgtacgaat gtggtcagca attactttaa atgccacatc ttgttcgttg
    13141 tttactaaat attgtttacc tgatactttt tcgatttcat tcattatagg cataaataaa
    13201 tctgtttcat agttagtacg tacattttga gaaactgagg ccatacgctc aagccccatg
    13261 ccggtatcaa tgtttttatt aggtaatggt gtgtaactat gatctttatt atgattgaat
    13321 tcactaaata ctaagttcca tacttcaaga tagcgttcat tttctccacc tggatacatt
    13381 tcttctgccg gatcgtcttg tccatatgct tctccgcgat catagaaaat ctcagtgttc
    13441 ggtcctgaag gtccttcacc aatatcccag aagttacctt caatgcgaat aatacgactt
    13501 tcttcaagcc caatatcttt atgccaaatg ttgtatgctt ccatatcttc cggatgaatc
    13561 gtaacgtaca atttatctgg ctccataccc atccatttat cactcgttaa aaattcccaa
    13621 gcaaattcaa tcgcttcttg tttaaagtaa tcaccaattg agaagttacc taacatttca
    13681 aagaatgtat ggtgacgcgc tgtgaaacca acattttcaa tatcatttgt acgaatagct
    13741 ttttgagagt ttacaattct tggcttttta ggtgtttcac gtccatcaaa atatttcttt
    13801 aatgttgcta cacctgaatt aatccataat aatgtatcat catcaattgg cactaatggt
    13861 gcagaaggtt caaccatatg tcctttttca acaaagaaat ctagatattt ttgtctaatt
    13921 tcactcgctt ttaacttttt catcatttac acatcctatt tactgttttt aaattaccat
    13981 tccataaaaa ttgatgacac agatagtcga tttgaaaaac tagtataaat caatatcact
    14041 ttttattatt aaaaaataaa aaacgcccat cctcaaaagg gacgaacgtt atcgcggtac
    14101 caccctagtt ataaatgcaa ttcaatacat ttatcacttt aaatcgacta tacagttgtg
    14161 cataaagtag cgttcactaa tgtttgttgt acttttcacc aaccagtaca tctctgataa
    14221 acaaatcatt aactactcat ctttatacga atttaattct attttagtta catttacgct
    14281 tgttgtcaac gttctataaa gtcatacggc gtgatttctc ccatattaat cattgggtca
    14341 attttaaaca ttgtagcttc cgttaataca tttgtatctg tttttgttga atcagacgta
    14401 acttcttcac tagcattcga tgacattggc gcttctactt gatcatctat tgtcgtttgc
    14461 gaagctcctg tatcattagt tgctgtattt tccagcatgt cttcatcttc tgaattaaaa
    14521 taatttttca acaatgtaca taattgtgtt aaacgcgctt gaccatttgt tttcaatcca
    14581 atatcaaatg cttctgaatc accaagtaaa actaaactgg ttttcgctct agttaaacca
    14641 gtatataata tcggtctttg taacattcta aaatactgtt taacaatagg catgataaca
    14701 ataggaaatt ctgaaccttg tgatttatgg attgatgtac aatatgcatg tgttaattcc
    14761 atcatatctt gtttcgtaaa tgtaatttca ttaccttcaa aatccacaac aagcacatct
    14821 ttattaaggg cattttcttt cgcccaaaaa ataccaacaa taactcctat gtcaccattg
    14881 aatatgttat catttggcct attaacaagt tgtaatactt tgtcaccttt tctaaagact
    14941 acatcaccaa actcaatttc tcgtgtgtct ttctttttag ggtttaaaat atcttgtaaa
    15001 acttgattta aacgtttaat accggcattt cctttataca ttggtgcaag cacttgaata
    15061 tcagccatag tatacccttt attaacagca ctagtaacta ccttctcaac aactgttggt
    15121 atttggtttg cctgacagtt aataaaactt ctatcatgaa aacgctgtgt aatatcaatt
    15181 ttctgaccca acttcattcg atgtgctaat tctataatgc ttgaaccatc ttgttgacga
    15241 tatacttcag tcagattaac tcgtggtata gctttcgatt caattaaatc tttaaatact
    15301 tgaccaggac ctacagaagg caattggtcc tcatcaccta caaatatcaa ttgtgcatct
    15361 aaaggaactg cacttaaaaa ttggtggaac aaccaagtat ctaccataga catctcatca
    15421 atgattatga gtcgtgcgtt aatttcattt tctaatatat cctctggctt tgtgtcttga
    15481 ttccaaccta ttaaacgatg aatcgtcata gcttctaatc cagttgactc ttgtagtctc
    15541 ttggacgctc ttcctgttgg cgctgctaat acaactggat aatcatcatt gacataatca
    15601 tcataatcta atgataagcc atgaatctca gcatataatt ctacaatgcc tttaattact
    15661 gtcgtctttc ctgttcccgg tccaccggtt aatagcatca ccttagaatt gatagccgtt
    15721 tgcaaagctt ctttttgtga agctgcatag ttcacttgat tcgcatcctc tatttcgcca
    15781 atatgcattt gtaaatctga ctgttcaatt tccgtaagtt tatttgtatg cgtctttatt
    15841 ctgaataagt tttgaacact tttgatttca gaataataca aacttggaat tgcaacttgt
    15901 tcattgtcaa taattagtcg tttttcctca tttaagtatt gcaacatttc gtctaatttt
    15961 tcaggttcga tgacctcttc atcttgataa tttaagacat caaccgttaa atctataaca
    16021 acattgatag gcaaatatgt atgtccctgt ttaatacatt cttcttctaa cgtatagagc
    16081 aacgcagctt ttaatcgttc attatcgtta taagcgatac caatatttct agcaagttga
    16141 tctgctttat taaaaccaat acctttaata tcataaatca attgatatgg atttcgatct
    16201 aaaatagtca gtgtatcacc gagataaaac tgataaattg ccattgaaag tttaggacca
    16261 aaccctaaat catgtaaacg aatcattatt ttttcagatt cttgatttgc tgaaatttgt
    16321 tctgcaattt gtttctgttt ctttttagat aatcccgaaa ctttttctag aactgaatgg
    16381 tcatctaata tatcgtttat cgcattgtca cctaatgtat taacaatatt ttgagctgtc
    16441 tttttaccta cacctttaaa caaatcactt gataaataac ttataattgc ttctttcgtt
    16501 tgtggcattt ctttttcaaa agtctctgct tttaattgtt taccataacg tggatgatca
    16561 acaacttgcc ctttaaatgt gtagacatcg ccttcaacaa tattcggaag aaaccctaca
    16621 acagttggca ttgtatcaaa gtcttcattt gtttcaatag tatctacttt aagcactgta
    16681 taaaaattat cactgttttg aaacaatatc gcttcaacag tacctttgat cattgaataa
    16741 tcaaatagtg tagggtctga catgttactc ctcctctttc attttagtga atgttttcag
    16801 cgcatgctga cttaataagt gtttagggtc gatagtcaca gcttctttaa aatgagttat
    16861 ggcttcatca atattctcat ttttcataaa taacgctaaa cccaaattgt atcttgcatc
    16921 aacatgattt ttatcaatcg ttaatacatg tttaagttga gttatggctt cattaaacat
    16981 ctctaattga cataatacaa gaccatattg aaattgaact tctgcatctt tgtctttatc
    17041 tagttctgca gcagtcatta aatacggcaa tgccagctta aatgattcta actgattaaa
    17101 cgccataccg atcatataat tacaatcaac ttgttcaatc tctgtttgta atgcttgttg
    17161 atataattta atagattctt gataacgttg ctgattataa tatacatttg ctagattata
    17221 aaatacgacg ccattcttcg gatctattgt taaagctttt tggaaaaaac gctctgcctt
    17281 ttcaatctca ttcgcatcag caagtactat gccggcatta atataatttt caataattgt
    17341 aggatcttct tcgatatttc cgaacaatgc ttgtaacgct tcttctattt ttccattttg
    17401 tatgtattga tatattgttt gttgatctat catttacgaa cctcatttct catcaattat
    17461 aacatcttga taaattgtat gtctcgaatc acttaacaac gaataaaata taatctaata
    17521 tcatcttcat tcatgaaaaa gcgggaatgg aatggaaatg ctaaagaacc attaacggtt
    17581 tattatgtaa tggttcttcc acattagcca ccactattat gtacttaaaa taggaataca
    17641 taattagact catgcatagg ggtgagacag aaatgatatt tttacaaaat taatttgtta
    17701 tccccaactc tcattgcctg tagaatttct ttacgaaatt ctctgtgttg ggccccgcca
    17761 acttccattg cctattaaaa ttctttacga aattctctat gttggggccc cgccaacttg
    17821 cacattattg aaatctgatt ttccgtcagc ttttatgttg ggggcccgtc cccaacttcc
    17881 attgcctgtt aaaattcttt acgaaattct ctgtgttggg ccccgccaac tcgcacatta
    17941 ttgaaatctg actttccgtc agcttctgtg ttgggggccc gccaatataa cattgtagag
    18001 cctaggacat tgtgatgtcc cagactctat catcatgaat tattctcatc aaaaactgtc
    18061 tttcgtcatt tccaacgctg aaacttcaaa taagtaattt attgttgtca ttgcttaaac
    18121 aacataattt aattgacctt catttttgaa cacatcgtca attgttgctc caccaagaca
    18181 cacatcacct tgataaaaaa caactgcttg tccaggtgtg attgctctta ctggctcagc
    18241 aaaagtaaca cgtagtgcat ggtcgttttc acgtttcaca aaaactttcg tatctttttg
    18301 gcgatatcta aatttagctg tacattcaaa accttgatct aagtcattat cttctggatt
    18361 tacaaatgaa tagtctgaag caattaagta gtcactgtat aatgcatcgt gatggaatcc
    18421 ttgttctaca tataaaacat tatcttttag gtttttaccg acaacaaacc aaggatcgcc
    18481 atctccacct atacctaaac catgtctttg tcctattgtg taatacatca acccactatg
    18541 tttacccatt ttcttaccat caagtgtaat catatcaccc ggttgcgcag gtaaatattg
    18601 agataaaaat gttttaaagt ttttttcgcc gataaaacaa atgcctgtag aatctttttt
    18661 cttagcagta acaagtcctt gttcttcagc aattcgacgc acttcactct tttcgatgtc
    18721 gccaattggg aacatcactt ttgaaagttg ttgttgagat aattgattca agaagtatgt
    18781 ttgatcttta ttattatcta caccacgtaa catttcaaca tgaccatctt catgacgatg
    18841 tatgcgtgcg taatgtcctg ttgctacata atctgcacct aaattcatcg catgatctaa
    18901 aaaggcttta aacttaattt ctttattaca cataacgtct gggtttggag tacgaccttt
    18961 tttatattca tctaagaaat acgtaaagac tttatcccaa tattcttttt caaaattaac
    19021 agcgtaatat ggaatgccaa tttgattaca cacttcaata acatcgttgt aatcttcagt
    19081 tgcagtacat acgccatttt cgtcagtgtc atcccagttt ttcataaata tgccaatgac
    19141 atcataacct tgttctttta agacgtgggc tgttacagaa ctatctacac cgcctgacat
    19201 accaacgaca acacgtatat ctttatttga caattatgac tcctccttaa atttaaaata
    19261 tattttatga atttcagcta caattgcatt aatttcattt tcagtagtca attcgttaaa
    19321 actaaatcga atcgaatgat ttgatcgctc ttcatcttcg aacattgcat ctaaaacatg
    19381 cgacggttgt gtagagcctg ctgtacatgc agatccagac gacacataga tttgtgccat
    19441 atccaacaat gttaacatcg tttcaacttc aacaaacgga aaatatagat ttacaatatg
    19501 gcctgtagca tccgtcattg aaccatttaa ttcaaatgga atcgctcttt cttgtaattt
    19561 aactaaaaat tgttctttta aattcattaa atgaatattg ttatcgtctc gattcttttc
    19621 tgctaattgt aatgctttag ccatcccaac aatttgtgca agattttcag tgcctgcacg
    19681 gcgtttcaat tcttgttcac cgccaagttg aggataatct aatgtgacat ggtctttaac
    19741 taataatgca ccgacacctt ttggtccgcc aaacttatga gcagtaatac tcattgcgtc
    19801 gatctcaaat tcgtcaaact taacatcaag atgtccaatt gcttgaaccg catcaacatg
    19861 gaaatatgca tttgtctcag caataatatc ttgaatatca taaatttgtt gcactgtgcc
    19921 aacttcatta tttacaaaca taatagatac taaaatcgtc ttatctgtaa ttgtttcttc
    19981 aagttgatct aaatcaatag cacctgtatc atcaacatct agatatgtta catcaaaacc
    20041 ttctcgctct aattgttcaa aaacatgtaa cacagaatga tgttcaatct tcgatgtgat
    20101 aatgtgatta cccaattgtt catttgcttt tactatgcct ttaattgccg tattattcga
    20161 ttctgttgcg ccactcgtaa atataatttc atgtgtatct gcaccaagta attgtgcaat
    20221 ttgacgtctt gactcatcta aatatttacg cgcatctctt cccttagcat gtattgatga
    20281 tggattacca taatgcgaat tgtaaatcgt catcatcgca tctactactt caggttttac
    20341 tggtgtggtc gcagcataat ctgcataaat ttccatgttt ggacactcct cacaatttta
    20401 tcaatgttcc aataatagca ccttacatac tatttttcta cttttctgtt taactttatt
    20461 tataatgttt ttaattatat tttaccattt tctacacatg cttttcgata ggctttttaa
    20521 gtttatcgct ttattcttgt cttttttata aattttagta tttgaagata ttttatcatt
    20581 tgtaaaatgt aacgtactat tattttggtt atgagcaatt taatatttat ctggttattc
    20641 gattggtata cttcttatat caaaaaaagg aaggacgata taaaaatggc gattaaatat
    20701 tcagcattaa accttgtccc tattcgagaa ggtgaagatg aacgaacagc aattaatgat
    20761 atggttaagc tcgcacaaca tttagacgaa ttatcatatg aaagatattg gattgctgaa
    20821 caccataacg ctcccaacct agtaagttca gcaactgctt tattaattca acatacgtta
    20881 gaacatacga aacacatacg tgtaggttct ggaggaatca tgttacctaa ccatgctcca
    20941 ttaatcgttg cggaacaatt tggcacgatg gcaacattat ttccaaatcg tgtcgattta
    21001 ggattaggac gtgcacctgg aacagatatg atgaccgcaa gtgcattaag acgagatcaa
    21061 catgatggtg tttataaatt tccggaagaa gtttcattat tacaacaata tttcggcccc
    21121 gctcaccaac aagcatatgt tcgtgcttat ccagcagtag gtaaaaatgt gcctttatat
    21181 attcttggtt cttcaacaga ttctgaacat ttagctgctc gcaaagggct tccatatgtg
    21241 ttcgctggac attttgcacc tcaacaaatg aaagaagcta tcgaaatcta caaaacgtta
    21301 tttgaacctt ctgatgtatt agacgaacct tatgttattg tatgtttaaa tacaatcgtt
    21361 gctgaaaatg atgacgaagc acaatattta gcttcatcta tggcacaagt aatggttagt
    21421 atcactcgtg gcagaatgca gcccgttcaa ccgccaacac atgaactgca aaatatatta
    21481 acgccgagag aatatgcgat ggctatggaa agacagaaaa tatcattaat aggttcagaa
    21541 aatactgttc aacaaaaaat tcaagatttt atggaaactt atggtgaagt caacgaaatt
    21601 atggctataa gttatattta tgataaagat atgcaattag actcttatcg tcgattcaaa
    21661 aatgttataa atcagttaaa tgaaaaaaac actttataat gtgataaata aactaagtga
    21721 aagtatgtat ccataatatt aataaaaata aacag
//
LOCUS       DAKSQE010000037        21358 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.037, whole genome shotgun sequence.
ACCESSION   DAKSQE010000037 DAKSQE010000000
VERSION     DAKSQE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21358
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.037"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(432..504)
                     /locus_tag="O0V33_001210"
     tRNA            complement(432..504)
                     /locus_tag="O0V33_001210"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(469..471),aa:Asp,seq:gtc)
     gene            complement(512..586)
                     /locus_tag="O0V33_001211"
     tRNA            complement(512..586)
                     /locus_tag="O0V33_001211"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(551..553),aa:Glu,seq:ttc)
     gene            complement(1016..2299)
                     /locus_tag="O0V33_001212"
     CDS             complement(1016..2299)
                     /locus_tag="O0V33_001212"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCX8975855.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTSVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
     gene            complement(2578..3978)
                     /gene="dnaB"
                     /locus_tag="O0V33_001213"
     CDS             complement(2578..3978)
                     /gene="dnaB"
                     /locus_tag="O0V33_001213"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCX8975856.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            complement(4010..4456)
                     /gene="rplI"
                     /locus_tag="O0V33_001214"
     CDS             complement(4010..4456)
                     /gene="rplI"
                     /locus_tag="O0V33_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCX8975857.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            complement(4453..6420)
                     /gene="gdpP"
                     /locus_tag="O0V33_001215"
     CDS             complement(4453..6420)
                     /gene="gdpP"
                     /locus_tag="O0V33_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HCX8975858.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIVLFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTDHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGESTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            complement(6435..7373)
                     /locus_tag="O0V33_001216"
     CDS             complement(6435..7373)
                     /locus_tag="O0V33_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HCX8975859.1"
                     /translation="MGVAYVFSKIQPKATIIATITLVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGISALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RILYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPILRKFKISTPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LIIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            complement(7666..8634)
                     /locus_tag="O0V33_001217"
                     /pseudo
     CDS             complement(7666..8634)
                     /locus_tag="O0V33_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     regulatory      complement(8694..8792)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(8997..9326)
                     /locus_tag="O0V33_001218"
     CDS             complement(8997..9326)
                     /locus_tag="O0V33_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCX8975860.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     AALRFFF"
     gene            complement(9323..10018)
                     /locus_tag="O0V33_001219"
     CDS             complement(9323..10018)
                     /locus_tag="O0V33_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCX8975861.1"
                     /translation="MTTHLSFRQGVQECIPTLLGYAGVGISFGIVASSQNFSILEIIL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     TLGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMER"
     gene            complement(10665..11951)
                     /gene="serS"
                     /locus_tag="O0V33_001220"
     CDS             complement(10665..11951)
                     /gene="serS"
                     /locus_tag="O0V33_001220"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCX8975862.1"
                     /translation="MLDIRLFRNEPDTVKSKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDNKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRCVILCTGDIGFSASKT
                     YDLEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
     misc_binding    complement(12041..12256)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(12329..13843)
                     /gene="hutH"
                     /locus_tag="O0V33_001221"
     CDS             complement(12329..13843)
                     /gene="hutH"
                     /locus_tag="O0V33_001221"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HCX8975863.1"
                     /translation="MTLYLDGEILTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHAVRNFQEQINVAARMRDWLEGSTLTTRQAEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYDEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH"
     gene            14169..14981
                     /locus_tag="O0V33_001222"
     CDS             14169..14981
                     /locus_tag="O0V33_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCX8975864.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVYRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
     gene            complement(15069..17729)
                     /gene="gyrA"
                     /locus_tag="O0V33_001223"
     CDS             complement(15069..17729)
                     /gene="gyrA"
                     /locus_tag="O0V33_001223"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCX8975865.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDSSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELEAILADEEVLLQLVRDELTEIRDRFGDERRTEIQLGGFEDLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELGNDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLRKGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDADEVNEDEQSTVSEDGTEQQREAVVNDETPGNAIHTEVIDSEE
                     NDEDGRIEVRQDFMDRVEEDIQQSSDEDEE"
     gene            complement(17766..19700)
                     /gene="gyrB"
                     /locus_tag="O0V33_001224"
     CDS             complement(17766..19700)
                     /gene="gyrB"
                     /locus_tag="O0V33_001224"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCX8975866.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANQIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            complement(19710..20822)
                     /gene="recF"
                     /locus_tag="O0V33_001225"
     CDS             complement(19710..20822)
                     /gene="recF"
                     /locus_tag="O0V33_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCX8975867.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEDIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            complement(20819..21064)
                     /gene="yaaA"
                     /locus_tag="O0V33_001226"
     CDS             complement(20819..21064)
                     /gene="yaaA"
                     /locus_tag="O0V33_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCX8975868.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
BASE COUNT         6503 a         4221 c         3222 g         7412 t
ORIGIN      
        1 ttttccaata taaacttata tattaatatt ttttattagt ttctcacggc ggattgtgat
       61 aatgatattt aacacaataa tgaggtctct attccaataa taaaattagg gtaatggcaa
      121 cttctagttg cacgtcattg ctcagaaaat aaatatcatt attcattact ttaacaattg
      181 atgttatgga ggattggtgg catatcacaa cgaagaaata tgttgacagt agcatatgtt
      241 tgtacgtcta aaaaagattt tattgtctac gtttatcttt catatccaac cactaagtta
      301 cttgaggttc atacattata aagaaaatgg acgaacagat aaatcaatat gattatcatt
      361 cgtccatatg tcttttatta atataatatt aaacgcaaaa aaatgcctat cctcttggat
      421 aggcatttgt acggtctcga cgggaatcga acccgcgatc tcctgcgtga caggcaggcg
      481 tgttaaccgc tacactacga gacctaaaaa atggtgactc ctacgggact cgaacccgtg
      541 ttaccgccgt gaaagggcgg tgtcttaacc gcttgaccaa ggagccgttc ttaagcacaa
      601 gatagattat agcacagcca atcttgccat gacaagatat tttttacata attttgctat
      661 tattttttga agaatgttat aagtgaaaaa ttgcatttta ttggcagtct atttattata
      721 ataattacgc tatttttgaa atttttctat tttagatact ttcattcaca cttgcgtcta
      781 ctctatttta atagctattt tcgacatcag acttattaca ttcaagtatt tcatcttaat
      841 tttcgttaaa tatcatctaa tcttttaaat tatgactagc aaatatcacc tttacgttag
      901 taatttttat ctaaataatc gaatcgatgc agaagcaaac atagcaaaag cctcctacca
      961 ataaagatag aaggctcatg tatttataat cattgtgtat gacttatata aagttctacc
     1021 acaattcttt taataggttt gtttgttctc tatctggacc aactgagaag atagaaattt
     1081 gtacattaca taattctgaa atacgctcta aatatttacg tgcattttca ggtaattctt
     1141 ctaaagtacg cacacttgtt acgtcttctg tccaacctgg taactcttca aagattggtt
     1201 tacaacgttt taattgatct aagtttgctg ggtactcagt aatttcttta ccgtctaatt
     1261 cataagctgt acaaattttc actgtgtcta ggcctgttaa aacatcaatt gagttaatag
     1321 ataaatctgt aataccactt acacgacgag agtgacgtaa tacaactgaa tcaaaccaac
     1381 ctacacgacg tggacgtcct gttgttgtac cgtattcacg accaacttct ctaatatgat
     1441 gtccatcttc atcgaataat tcagtaggga atggaccatc accaacacgt gatgtataag
     1501 ctttacatac accaattacc tttgaaacga atgtaggacc tacacctgta ccaacagtaa
     1561 cgttacctgc aattggatta cttgatgtaa cgaatgggta tgtaccatgg tcgatatcta
     1621 acattacacc ttgcgcacct tcgaaaagta ccttttcatc tgctacaaat gcatcgtcta
     1681 agatttttga tgtgtctgtt acaaattctt ttagacgttg acctgctgca taatattctt
     1741 caaagatatc atcaaatgat ggacatgttt cgttaaacat acctttaaaa tatgcttgtt
     1801 tatattcaat gtttgatttt aacaatcttt cgaatgtttc tttttcaagt aaatctgcca
     1861 tacgaatacc gatacgttga actttgtcta catatgctgg accgatacct tttttagttg
     1921 taccaatctt attatcaccg cgtaaacgtt cttcatattc atcttgtgct aagtgatatg
     1981 gtaaaatcac ttgcgcacga ttagatatac gtaaattact tgtaggaatg ccacgttcat
     2041 ttaatccgtc taattctttc aatagtgcaa ctggatcaac aacgactccg ttaccgatta
     2101 ccgctaattt gtctttgtaa aagataccag atggtactaa atgtaattta tatgtttctc
     2161 cgccaaattg aatggtatgg cctgcattat taccacctga aaaacgcgcg ataacatctg
     2221 actgttctgc caagaaatcc gttatttttc cttttccttc gtctccccat tgtgtcccaa
     2281 ctactacgat tgatgacatg tgagcacctc caagtttttc tcatttaacc atttgatatt
     2341 ctaccaatat cgagaccata atgcaaataa aaatcgaaca ttgatttttg gcctttttaa
     2401 aagaaaaatc attttaacaa aaagtcttca atgctctaaa aaccctttaa cagaggcatt
     2461 gacaacacta attcatcgct tccaatacac aaacttaaca tatatcaacc cgttcagaac
     2521 aaaataaaaa ccgtacaatt ttatcattaa aatgattatt gtacggaaaa acttttttta
     2581 catcatatca gcatgtgcat aatcgatatc tgtaaattta ttatattgtt tcataaagtg
     2641 taacttaact gtgcctgttg ggccattacg ttgcttagca atgatgattt caatttcgcc
     2701 gttttcatca ttcgtttgtg gctcgaaacc accatcatcg tcatcatctt catcgccacc
     2761 acggttatag taatcatcac ggtataagaa tgcaacgata tcggcatctt gctcaatcga
     2821 accagattca cgaatatcac tcatcattgg acgtttatct tgtcgttgtt caacaccacg
     2881 agataactga cttaatgcga taactggaca ttctaattca cgggctaatg cttttaatgt
     2941 acgagagatt tcagaaactt cctgttgtct gttatcggac gcacgtgaac cactaccttg
     3001 aatcaactgt aagtagtcaa tcacaatcat gtctaagcca tgttcttgct ttaatcgacg
     3061 acatttagaa cgtagatcat taattcgaat acccggtgta tcatcaataa aaatcttcgt
     3121 acgtgataat ttacctaccg ctatagtaaa acgactccaa tcttcctcag tcatagtacc
     3181 ggttcttaag cggtttgagt caacattacc agaactacaa atcatacgtg tggctaactg
     3241 atcagcaccc atctctagtg agaaaatacc aactgtatac atatcttcat gcgttgcaac
     3301 tttttgtgca atattaagtg cgaacgcagt cttacctaca gatggacgcg ctgcaaggat
     3361 aattaaatca tttcggttga accctgctgt catttggtct aaatctcgat atcctgtagg
     3421 aatacctggt gtttgaccac tattttgatc aagctcttca gctgtttcat atacttgtcc
     3481 taagacgtct cgaatgtctt taaagccatc gctttcacga gaagatgata gctctaaaat
     3541 tcgacgttct gcatcactta aaatcgcatc tagttcaagt tcatcattat atccatcatt
     3601 ggcaatacta tctgcagttt gaatcaatct acgttttaat gcatgcttag aaacgatatc
     3661 agtataatac tgaacatttc gcgtcgttgg tacatttgta gataactctg caagatattg
     3721 cgggccaccc gcttcattca acgtaccttc cgtcgataat tgatccatca atgttacaac
     3781 atcaatttct ttattatctt catttaagtg catcattgca cggaaaatat gttgatgggc
     3841 acccctataa aatgactcag gaagcaacac ttcctgagta gtattaatca attctggatc
     3901 tataataatt gaacctaaga cagactgttc agcttcattg ttatgtggca tttgattttg
     3961 ctcatacatt ctatccatga atggttacac ctcttatttc aatccaactt tattgttcaa
     4021 ctgtgtgtac gcgaattgta ccttcaactt ctttatctaa tttaacaggt acattcgtat
     4081 atcctaggga atgaattcca tttggtaaat ccattttacg tttatcaatt ttaatatcat
     4141 gttgtgcttt tagtgcttcg gcaatttgtt ttgtacttac tgatccaaac aatttaccac
     4201 cttcaccagt ttttgctgat acttcaactt caatgttcga taaagtttct tttaatgctt
     4261 tagcatcttc aatttcttgt tggcgttctt gttttgcacg ttttttctgt aactctaatt
     4321 gtttaaggtt acctggtgtt gcttctacag cataattctt tttcaataag aagttatttg
     4381 cataacctac tggtacttct ttaacttcac cttttttacc tttaccttta acatcttgtg
     4441 taaaaattac tttcatgcat cttcactcct acttaattgt tctgtaattg cttgttgtaa
     4501 ttgtgctatc gcctcttcga ctgtcacacc tttaagttgt gttgcagcat tggttaaatg
     4561 tccaccgcca ccaagtgctt ccattgttaa ctggacattt actgaaccga gtgaacgcgc
     4621 agatatacca atcagattat cttcacgtct cgcaacaaca tatgatgctt caataccttc
     4681 taaacttaac agttcatctg ctgcttgtgc aactgttact ggatgataaa ttttatcgtc
     4741 tgaaccatgc gcaatggcta tgccattatc ttcaactttt acagttcgaa ttaattcaga
     4801 tcgattaatg taagtatcca catcatcttt taagaaatgt tgcgttaaaa tcgtatctgc
     4861 accatgtgca cgtaaataac tcgctgcatc gaatgttctt gatcctgttc gtaatgtaaa
     4921 gtttcttgta tctacaataa tacctgcata catcactgtt gattcaagac gtgttaaacg
     4981 ttgttctgtt ggttgatatt ccagtaattc tgttacaagt tctgctgttg aacttgcgta
     5041 tggttccata tatatcaaca atggattaga gatgaagctt tcaccacgtc tatgatgatc
     5101 gataacaact ttacggtttg ctttatttaa gacattttca tctaaaacca gttccggttt
     5161 atgcgtatca acaatcacta cggttgtctt agatgtcatc atatcccaag catcatctga
     5221 tgtaataaat cgctctctta actctggctt tttatcaatt tcgttcatca cgcgtcgtaa
     5281 tgttggatca atgtcagtct catttaatac gatgtatgct tctaaattat tcatcattgc
     5341 aaatctagac acaccgattg ctgcaccaat tgcatctaag tcaggacgtt tatgtcccat
     5401 gataatgact ttgtcaccct ctgcaagaat atcttttaac gcatgtgaga tcacacgtgc
     5461 tcttacacga gtacgtttct ccatcgggtc agtcttaccg ccatagaaac gcacattacc
     5521 attaatactt ttaattgcaa cttggtcgcc accgcgtcct aatgctaagt ctaggcctga
     5581 ttgtgataat tcacctaaat cgattaaatt ttcagtgctt tcaccaacac cgatacttaa
     5641 tgttaattgg gcacgataac caacactttt ttcacgtaat tgactcaaga tatcaaattt
     5701 agattcttct aagtcagcta atattttttg atttaaatag gctacgaatt gatcggaact
     5761 atacctttta aagaatatat tatactcagt tgcccatcga ctaatgacac gcgttaccat
     5821 tgaattgatt tccgaacgct gcgtatcatt catattttgc gtaatctcat cgtaattatc
     5881 taaaaataat gtcgcaatga ttggtttaga attttcatat agttcatttg tttgtacttg
     5941 ttcagttata tcaaagaaat aaaggcagtg atcattctca gaataacgta cttggaaatg
     6001 atactgatta tattctattt caacggattt cactctatct aattgcttta aaatgtttgg
     6061 aaatacttca tttacagatt cagaaatgac atttgcttcc atatgatctg tcataaattg
     6121 gttaacccat tcgatgtgat cattttcatc taaaacaatg ataccaattg gtaaatgttt
     6181 gattgcttta ttatttgttg ttgaaatttg cgcactcaaa ccatctacat aactatccat
     6241 tttcattaaa gcttgtctga ataaaacgat gctaacaata atcatcacga caagaacgat
     6301 agatgcaata agtgctataa gactattaaa gataaaccat acacccatta aaacaattgc
     6361 tgtgatgatc atgatgacaa atggtattag taaagctttc ttagtggact gccgattcat
     6421 tattccacct ctattcactt tttagaatta tttttcatga ttcgcttcaa attcaaactt
     6481 aaatcgataa caccaagtag tcctacaata tgcgtcgtag gtgtcagtat tgtaccgata
     6541 atcaatagta aaatcgttac tgcattcggc aaacctttcg ctttaccaaa gaaatgaata
     6601 acacttaaac cttgaatata catcactaat gataacacaa gttggaaatt taaaagaatg
     6661 ctctggaaca cactcggttg acctgtaaat aataaacata tgataacaat aatgtatatc
     6721 cataataaaa taccgctcat ttgccacgcg aaaagtggct taaatacagg tgtagagatt
     6781 ttaaattttc gtaaaatcgg aaatgtaacg attaagttaa ttaagacgat taaaaatgta
     6841 atgataatga tgaaacctgg taattgaacg gtcgcttgtc taaacccttc ttctaatatt
     6901 tgggtcatat tcgcatcggc accgctcatc gtaatcgctt catgtaatgt ttgcttgaaa
     6961 ggttttacta tgctcgctga tggtggaatt cttccgaatg tttgtagtaa cataaaggcg
     7021 attaatgaaa ttaagctcat cgctactgtt gttacgtaca atattctttc tttagacgtt
     7081 ctttctttga gcaattgacc aataattaaa cttgcaatta agactaatat gatagcactt
     7141 aaaacgaaag tattacctaa aacagttgtt ataattactg taataagtgc actaatcccg
     7201 aaagattgta ttgatttatt ccataaaacg atacctggta tggttgcaaa taatgcaaat
     7261 attagtccta aaccaggcac tagatataaa gctaaagcga caaataccaa cgtaatcgtt
     7321 gcaattattg ttgctttagg ttgtattttt gaaaacacat aagccactcc catattttta
     7381 actatagcta ttattttaac ctctttaatg gaatttaaca atttatagat tgtatgcttc
     7441 tatttcattt aattgaataa taactttcat gttttataag taattaacat actcatttga
     7501 atcgcttttg tgtgctttca ttttcaacat gattatttaa tcccactaca tagcaatcaa
     7561 gcttgatttt gatttacaat tcatttccac tctcatgtac tctagatgtt tttgaatatg
     7621 ataactgtga tttagtggct tcattctttg aaaatatata cattattact tgcgcttaaa
     7681 atgctttaaa tttaagaaat gatataagtt aggtgcccag gtactaaagt ttagtaggaa
     7741 tccatcatga ccaacgttat caggtacaaa gaaatgacga tgatatttaa aacgttcacc
     7801 taaagcacga acttgatcgt cgggatatag caaatcatct ataaagccca ttgttaagac
     7861 tttcgtttct aaatttttaa aaacatgcgt tacgtctgtg cgacctcggt caatgttgtg
     7921 actatccaat acatctagca gtgtcagata acaattcaaa tcaaatcatt ctttaaattt
     7981 attaccttga tgttgttgat atgcgactac ttcatccggc gtgaaacgtt catcataact
     8041 ttttgatgat cgatatgtca aaaaacctaa ttggcgtgca atacttagcc cttccttacc
     8101 accaagatga atggcctgac gcgcaatttc attgaaagct ctactataag atgatgttcg
     8161 acttgttgca gcaaggataa tggctttatc tacttcaaac tgttgattgt aaagtagttc
     8221 cattgcttgc attcctccaa gacttccccc tattaaaata ttaatcttgt cataaccaag
     8281 ggcttgtata cctcgttcat tcgctctgac tatatccctt aatgttaatt ttttaggaaa
     8341 atgagtgtcg tttaaaggtg agcttgaacc gaaaggacta ccaataacat caaatgttaa
     8401 aaattgataa tcgtgaatgg gtatatatcc cccatcaata atttctcgcc accaccccgg
     8461 ataatcatct gttccatatg ttaaatgatt gccagttaat gcatgacaaa ctacaactaa
     8521 tggttgtcca tgataaccga catgctcata tctcaaacgc aagttatcta tgacttcccc
     8581 agattctgta ataaatttcc ctagatttaa agtatctact gtgtaatttg tcattattct
     8641 ttcctcctta aacaaaaaaa cttctcaccc tattgaaaag taagaagtct ttatacttat
     8701 cattcgagta actcgttggt tttagcaccg tgctataaag tcggttgctg aagtatcaca
     8761 gggccaagtc cctcaacttc tcacaataag aaatatgaaa ttgttatgtg ttagttgaga
     8821 ttcagtgatg aattactttt atcatttaaa atgttgttat cattgtcatg cgttagccaa
     8881 atcgcttacg tatacacgat tcccaatttt aacatagacg atttgtatat cagaattttc
     8941 tgattactaa cagtttacct aagtttaaat atctgttcaa tgattttcag ttatttttaa
     9001 aagaaaaatc gtaatgctgc catgataaca atcccactaa taattgtaat agttaaacta
     9061 cgcgtgatta tagataaaat aaccgtcgga atgagcgcga taatgtaagg gatgtttaat
     9121 gtatacccct caccatgagg cgtctgctga ataatgctgt caatgacaag tgccgtaaat
     9181 agtgtgattg gaataaatga tagccatcga accacgacat caggcaattg tacttttgaa
     9241 atcatgataa aaggtataat tcgaattaat agcgttacga taccacacaa taaaataagt
     9301 attaacatgt tcatatgagt tatcatcgtt ccatcatcac tcctaacgct gctgaaattg
     9361 tggctgcaat taatattgct agatatgaag gcataaacat acttagcgat aacatcatta
     9421 ctatgacggc aataatgagt actatgtaaa ttcttaatcg cgatttagta attgattcaa
     9481 attgcgcaat ggccaaaaag ataaacatag ctgtgatagc aaagtctaac cctagcgttt
     9541 gcggatttga gatatattcg ccaaataaag ccccagctac acacgaaatt gcccaaaata
     9601 aatatgctgt aatattaaga ccatgcatcc agcgatcatt gatagcttct ccttttaaat
     9661 aaggtgtaat ggcgacgcca aacgtttcgt cagttactaa tgaacctaat ccaacacggt
     9721 tccaaaaccc atatgtcttg aagtttggtg caagcgacat acttaaaagg aacattcttg
     9781 aattaacgat aaatacagtt agtacaatcg ctgatatcgg tgtacctgct ataaacaacg
     9841 cgcacataat aaattgcgca gcaccagcat atataacaag acataataag ataatttcta
     9901 aaatactaaa gttttgagac gaagccacaa taccaaatga aataccaaca ccggcataac
     9961 ccaataatgt tgggatacac tcttgcacgc cttgtctaaa acttaaatgc gttgtcatct
    10021 caattacctc ctttgccact ttatatttat ataattacga aaaaataata tacctagtct
    10081 aatacagttc tataccctaa gcaataacat tagactacgg tttgtctgag gttagacata
    10141 ccggagtctt taaccagctt catattaaat ttttattttt gagaattttc atttttttat
    10201 ttaagactac ctctatattt tctgcggatt tggtgtttat ttttaagtac taattttata
    10261 attttttata tctaatgatg agtctgggat attgattcgt gtctcaaaca cttatactca
    10321 tcatcccctc ccccctactc catcgatatt actcatacta catcaacgaa attagttttt
    10381 atcgcttaat ttcctataat agtgatgctc aaaattgtta cgttttagat tgttttagtt
    10441 catcataatt atcccgtatt gttgctataa tgaaatgcgt tcaccccatt aaaccacaaa
    10501 cttaatttat tgttgttatg tgcattggct caatattata cttttactgc acaaaaaaag
    10561 tggcgacagc ttcgtcacca ctttttaaaa tattatttaa agtatcttgc ccttgcttta
    10621 agtatacgta gatatatact ttttaaagct tgtagctaaa gcctttattt aactggtttt
    10681 gaaatttgtg ttttaccacc cataaatggt actaatgctt ctggaattgt tactgttcca
    10741 tcttcatttt ggtaattttc aacaatagca gcaaatgtac gtccaactgc taaaccacta
    10801 ccatttaatg tatgtgctaa ttctggttta gctgctttgt cacgcttgaa gcggatgtta
    10861 gcacgacgcg cttggaaatc cgtacagttt gaacatgaac taatttcttt ataatcattg
    10921 tagcttggta accaaacttc taaatcatac gttttgcttg cactaaatcc aatatcacct
    10981 gtacataaaa taacacaacg gtatggtaaa cctaactctt ctaggattgc ttctgcgttt
    11041 gttgtcattt cttctaaagc attccatgaa tcttcaggtt gttcaaaacg taccatttcc
    11101 actttatcga attgatgtag acgaattaat cctcttgtat ctctacctgc tgatcctgct
    11161 tcactacgga aacatgcaga ttgaccagtg aatttttcag gaagtacacc tggttgaata
    11221 atttcattac gatagaaatt cgttaatggt acttcagcag ttggaattgt atataatcct
    11281 tctttttcca ctttaaataa atcttcttca aatttaggta attgacctgt accatacatt
    11341 gtatctgcgt tcacaagctg tggtaccatc atttctgtat aaccatgttg tgttgtatgt
    11401 tttgtaatca tatagttcat taaagcacgc tctaattgcg caccttcatt tgttaaatat
    11461 acaaaacgcg cacctgaaac ttttgctgca cgatcaaaat cagccatttt caattcttct
    11521 acaatatccc aatgtgcttt tggttcaaat gaaaactcac gtggtgtacc ccacttttta
    11581 acttcaacgt tatcttcatc agattcacct tgaggtacat catcacttat taaatttgga
    11641 atacgacaaa ggatacctgt cattttatta tcaatttcat ttaattgact atctttttct
    11701 ttaatatcgt cacctaatgt gcgcatttca gcaatcacat catcagcatt ttctttatta
    11761 cgttttttta atgcgatttc ttcacttact ttattacgac gtgctttcat ttcttctgtt
    11821 gcactaatta atttacgtcg ttgctcatcc aattctaaaa tttcatctac aacttttgga
    11881 tcatctccgc gtaattcaat tttgctctta actgtgtcag gctcatttct gaataatcta
    11941 atgtctaaca ttaaccttca tcctttccca aataattatc atttattatg gaatgacgta
    12001 cgtctttatt ttttagaaaa taaaaaaaga ccacatccct acaagggacg tggtctacgc
    12061 gttgccaccc tatttaacaa tttaagttat aaagatacac taaacctaaa ttgcacttca
    12121 ctaaaataac ggttatcacc gattgttctt ttaaataagt aggtagattc atatatatgt
    12181 tgattcttgt tcacactaac cacaagctct ctgatatcga acactatata ttacttgtcc
    12241 tacgaacaat gtcttattaa gttattttta atatagcaaa ctatatttgc tttttcaagt
    12301 aacgatttca aacatcactc atgtcgattt agtgacatgc agtcgtttga taaattgatt
    12361 gctttaaata ctgtgcaacc gcttcaatat ctttatgaaa ttgacggtca tgtgtaatgg
    12421 atggcacgat acttcgaaac tcatcatact tgcgacgtgt ttttggtgat aatccttcga
    12481 cacctttcaa ctctgctgct tgtaatgcaa taacacattc gattgccaac acacgtcttg
    12541 cattttcaat aatttgataa ccatgtctag cagctgtagt tcccatagat acatgatctt
    12601 cttggttcgc agatgaagtg atagaatcaa cactcgctgg atgcgctaaa gttttatttt
    12661 cagaaacgag acttgcagca gcatattgca taatcattgc gccactttgc aatcctggct
    12721 ctggactaag aaatgctggt aaatcaccat ttaattgagg atttactagt cgctctagac
    12781 gacgttccga tacgtttgct aattcactta cacctaattt aagatgatct aatgcaaaag
    12841 caataggttg tccatggaag ttaccacctg aaataacaaa cgtttcattt gcttcctcaa
    12901 atataagtgg attatcatta gccgcattca tttcaaattc caattgctgt ttaacataat
    12961 tgaatacttg aaaactcgcg ccatggattt gtggtataca acgcaacgta tatgcatctt
    13021 gtacacgtat ttctgcttgt cgcgtcgtta atgttgatcc ttctaaccaa tcacgcatac
    13081 gcgctgccac attaatctgt tcttgaaaat tacgaacagc gtgcacatca tgtcgatatg
    13141 catctataat gccattaaga gactgatgcg ttaatgcagc aatccattca gattggtaac
    13201 ctaaatcttc tgcttctata taactaatga caccttgagc tgtcatagct tgcgtaccat
    13261 taatcaatgc taaaccttct ttagcctgaa ggttcaaagg ttgtctattt aattctctta
    13321 atacatcgtc actatccttt tcttcccctc tgtacaatac tttcccttca ccaattaatg
    13381 ctaatgctaa gtgtgataat ggtgctaaat ctcctgatgc accgagagag ccttgctgtg
    13441 ggattatcgg tataatacgt tcatttataa aaaattgtaa ttgtctcact aattctaaag
    13501 tggcacctga atgacctttt aataatgtat tcaatcgtaa aatcatcatg actaatgcta
    13561 cttcttttga aaatggctca cctagtccac aggcatgtga gcgtatcaga ttcacttgta
    13621 attcattata ttgcgtcggg tctatacgta catcactaaa taacccaaaa cctgtagtga
    13681 taccgtaaac cgtttcctca ttttcaataa tgcgttcaac tactgctcta ctttttttga
    13741 cacgttctaa cgcatcatca ataatttcaa tctttgattg ttgttgtaaa aatgatttaa
    13801 tatcctcaat tgttagtatt tcaccatcta aatataaagt catatatgtt acccccttgt
    13861 ttatattaag taacccatcc ctcttgaagt atacgttttc atttttattg aaacaatggt
    13921 tttacgtaca tttataacct attatcagag cactattgta gtgcgttaaa ggatattaag
    13981 attgttgtaa gcatatttaa taatttatct attgacgaat tgcatataca ggtatagtat
    14041 tttctattgt atttaacgac aaataataat gaattcagaa atttataata cattttgtta
    14101 agagttacta tatattttta aaattgaata aattcggaaa aggcttttac atgggaggtt
    14161 atatcactat ggaaacgtta aattctatta acattcctaa gcgtaaagaa gattcacata
    14221 aaggtgatta tggcaaaatt ttattaattg gtggatctgc taacttaggt ggtgccatta
    14281 tgttagcggc tcgtgcatgt gtatttagcg gtagtggttt aatcactgta gctacacatc
    14341 caacaaatca ttcagcatta cattctcgtt gcccagaagc gatggttatt gatattaatg
    14401 atacgaaaat gttgacgaaa atgattgaaa tgactgacag tatattaatt ggtccaggtc
    14461 ttggcgttga tttcaaagga aataatgcca ttacattcct actacaaaat atacaaccgc
    14521 atcaaaattt aatcgtagac ggcgatgcga ttacaatatt tagtaaactg aaaccgcaat
    14581 tacctacatg tcgtgtgatc tttacaccgc acctcaaaga atgggaacga ttaagtggta
    14641 ttcctattga ggaacagaca tatgagcgta atcgtgaagc agtttatcgt ttaggcgcaa
    14701 ctgttgtact taaaaaacat ggtactgaaa ttttctttaa agatgaagac tttaaattaa
    14761 caatcggtag cccagcaatg gcgactggtg gtatgggcga tacacttgct ggtatgatta
    14821 caagttttgt cggtcaattt gataacttaa aagaagcggt tatgagtgcc acatatacac
    14881 atagttttat tggcgaaaat cttgcaaaag atatgtatgt ggtgccacca tcaagactta
    14941 tcaatgaaat accttatgca atgaaacaat tagaaagtta gtcattatta atcattgaat
    15001 atagtacagc attactttct agcataaaaa taagactccc ctacatatag ggaagtctta
    15061 tttttttatt attcttcatc ttcatctgat gattgttgta tatcttcttc aacacgatcc
    15121 atgaaatctt gtcttacttc aatacgtcca tcttcatcat tttcttctga atcaatcact
    15181 tcagtatgaa ttgcatttcc tggtgtttca tcatttacaa ccgcttcacg ttgttgttca
    15241 gtaccatctt cagatacagt agattgttca tcttcattta cttcgtctgc atcctctttt
    15301 actttagcaa ccgttgaaac aaattgatca tcgcctaagc gaattaagcg aacaccttgt
    15361 gctgcacgac cattttgaga aatatctgca acatctagtc gaataatgac acctgcatta
    15421 gtaacaatca ttaaatcttc ttcaccagtt actgtagtga tacatacaac attaccatta
    15481 cgctcagtaa tcgtagctgt tttaatacct ttaccaccac gatttgataa acgatagtca
    15541 ttaactggcg tacgtttacc ataaccattt tcagtaacta ctaatacttc atcaacactg
    15601 tttgcatgag ctacatcaag ccctacaact tcgtcacctt tacgaagtgt aatacctttc
    15661 acacccgttg ctgtacggcc taaaggacgt aatgttgatt cagggaatcg aattaatgat
    15721 gcatgtgatg taccaatcaa gatatcttct tgaccacttg ttaagcgaac tgcaattaac
    15781 tcatcatctt ctctgaacga aatcgcaatc ttaccatttc tatttattct tgagaagtta
    15841 cttaatgctg aacgtttaac gacaccacgt ttagttgcaa acactaagaa gttgtcttca
    15901 ctttcaaggt ctttaacagc aatcattgta ctaatgactt catcatttcc aagttcaata
    15961 gcattcacta caggaatacc tttagactgt cttgataact caggcacttc ataacctttt
    16021 agtttgtata cacgaccttt gttagtaaag aacaatacat ggtcatgtgt acttaaagtt
    16081 accaattgac tgacaaaatc ttcttccaat gtattcatac cttgaacacc acgaccacca
    16141 cggttttgag cacgatatgt agataccggc aaacgtttaa tgtagttatt atggctcaaa
    16201 gtaattacaa tttgttcttc tggaattaag tcttcgtcct ctaagtcttc aaatccacct
    16261 aattgaattt ctgtacgacg ctcatcaccg aaacgatctc taatttcagt caattcatct
    16321 ctaactaact gtaataacac ttcttcatca gctaagattg cttctaattc actaatataa
    16381 tttaataact cattatattc agcttcaatt ttgtctctct ctaaacctgt tagacgtctt
    16441 aaacgcatgt ctaaaatagc ttgagcttgt ttttcagaaa gtttgaagcg ttgttgcaag
    16501 ctttccattg caactttatc tgtatctgac tcacgaatcg ttgaaataat ttcatcgata
    16561 tggtcaagtg cgatacgtaa tccttctaaa atgtgggcac gatctttagc tttacgtaag
    16621 ttatattgcg tacgtcttct aacaactgtc ttttgatgct ctaaataatg taccaacgct
    16681 tcttttaaat taataagctt cggtctacca tttacaagtg caatcatatt cacaccaaat
    16741 gatgtttgaa gaggtgtttg tttgtataag ttatttaaaa tgacactagc atttgcatcc
    16801 ttacgcacat caataacgac acgcacacca gtacgtaaac ttgtttcatc acgtaaatca
    16861 gtgataccgt caattttctt gtcacgaacg agctctgcaa ttttttcaat catacgagcc
    16921 ttattcactt ggaaaggaat ttcagtgaca acaatacgtt gacgtccgcc tccacgttct
    16981 tcaataactg cacgagaacg catttgaatt gaaccacgac ctgtttcata tgcgcgtcta
    17041 ataccactct tacctaaaat aagtccagca gttgggaaat caggaccttc aatatcttcc
    17101 attaactcag caattgaaat atcagggttc ttacttaagc taagtacacc attgattaat
    17161 tctgttaagt tatgtggtgg aatattcgtt gccataccta ccgcgatacc tgatgcacca
    17221 ttggctaata agttagggaa tcgagcaggt aagactgacg gctctctttc attaccatca
    17281 tagttatcga taaaatctat tgtatcttta ttaatatcac gtaacagttc aagtgtgatt
    17341 ttagtcatac gcgcttcagt ataacgcatt gctgctgcgc catctccatc cattgaacca
    17401 aagttacctt ggccatcaac aagcggataa cgataactga aatcttgagc catacgtacc
    17461 attgcttcat aaatagatga gtcaccatga gggtgatatt tacccattac gtcaccaacg
    17521 atacgtgctg attttttata tgatttatcc ggtgtcatac cttgttcatt taatccatat
    17581 agtatacgac gatgtactgg ttttaaaccg tcacgaacat ctggcaatgc acgagcaacg
    17641 ataacactca tcgcataatc taaaaatgat tcacgcattt cactggtaat atttcgttca
    17701 tttattcttg attgaggtaa ttcagccatc aagagttcct ccttcaaaag ttcagttcac
    17761 agcgcttaga agtctaagtt tgcataaact gcattatctt ctataaattg tctacggttt
    17821 tctacaacgt cacccattaa catttcaaat gtttggtccg cttcaatcgc atcttcaagt
    17881 tttacttgta aaagagcgcg gtgctcaggg ttcattgttg tttcccataa ttgatctgca
    17941 ttcatttctc caagaccttt gtatcgtgca atagaccatt ttggtgttgg attcaattca
    18001 gatttaagtt tatcaagttc cctatcattg tatacataat acttttgttt accttgtgtc
    18061 agtttataca acggtggctg tgcaatatac acatagcctg cttcaattaa cggtctcata
    18121 aatcgataga agaatgttaa taacaatgtt ctaatatgcg ctccatccac atcggcatca
    18181 gtcataatga cgattttgtg atatcttgct ttcgctagat caaagtcgcc accgatccct
    18241 gtaccaaatg ctgtgatcat ttgacgaatt tcattgttat tcaaaattct atctaatcgt
    18301 gctttttcaa catttaatat cttacctcgt aatggtaaaa tcgcctgcgt tctagagtca
    18361 cgaccagatt ttgtagaccc cccagcagag tccccttcga ctaagaatat ctcacattct
    18421 tcaggacttt tactagagca atcggctaat ttacctggaa gacttgctac atctaacgct
    18481 gatttacgac gtgtaacttc acgcgctttt ttcgcagcaa cacgtgcacg tgccgccata
    18541 ataccttttt caaccactgt acgtgcaact tgtggatttt catataaaaa tcgttcaaag
    18601 tgctctgaga ataatttatc tacaacttga cgcacttcag aattacctaa ttttgtcttc
    18661 gtttgacctt cgaattgagg atcaccatgt ttgatagata taattgctgt cataccttca
    18721 cgtgtatctt caccagaaag tctatctttt tcttctttca taatcttgct acttaaacca
    18781 taactattta agacacgcgt taatgcacgt ttaaatccgt cttcatgcgt accaccttcg
    18841 tacgtatgaa tgttatttgc gtaagttaaa agatttgtgg catatcctga gttatattga
    18901 atcgcaattt ctacttcaat atcatcttta gattgatgaa tataaattgg ctcatcatga
    18961 ataggttctt tattttcgtt caataactca acataagatt taataccgcc ctcatagtga
    19021 taggagtctt ctctaacgtt ttcttcatca cgttcatctc ttaatgtgat ttgaattcct
    19081 ttgtttaaga aagcaagctc tctaatacgt tgctgtaatg tttcatagtt gtatacagtt
    19141 gtctctgtga agatttctcc atctgcttta aaacgaatga cagtacctgt cttatcagtt
    19201 gtgccaactt cttttaagtc aaattgaggt acaccttttt tatatgcttg atgatatata
    19261 gtctcatttc tgtgtacata tacttctaag tcttgtgaca atgcgtttac aactgatgaa
    19321 ccaacaccat gtaaaccacc agatactttg tatccgccac cgccaaattt accaccagca
    19381 tgtaaaacag ttaaaataac ttctacagct ggacgtccca ttttttcttg aatatcaact
    19441 gggataccac gtccgttatc cgttacttta atccagttat ctttttcaat aacaacttca
    19501 atttgatttg cataaccagc taatgcttca tcgatactat tatcgacaat ttcccacact
    19561 aaatggtgca aacctctctc tgaagtcgat cctatataca tacctggtct tttacgtact
    19621 gcttctaaac cttctaatac ttgtatttgc ccagcaccat aattatccgt gttgtttaca
    19681 tctgacaatg cagtcaccat cgctttctgt tactttataa tttcaccttg attaatacga
    19741 tacaatttag cgttattcat gatttcatga tcaataccat ctacagatgt cgtagtgaca
    19801 aatgtttgta ctttgtgctg aatcgtactt aataaatgcg tttgacgcga atcatctaat
    19861 tcactgagta catcgtctaa taataagatg ggatattccc caacttcgat attcattaac
    19921 tcaatttcag ctaatttaat ggacaaagcc gttgtacgtt gttgtccttg agaaccatat
    19981 gtttgagcat ccatgccatt cacatcaaaa cttatatcgt ctcgatgtgg tccgaataag
    20041 ctaatgcctc gttctttttc tctttgcata ttatcgctaa gaatagacat aatgtcttca
    20101 agtcgtgccg cttcattttg agcataatca aatttaagac taggtaaata attcagcgac
    20161 aacgcttctt tatcatttgt gataccagca tgaatcggtt tagctaacga ctctagctct
    20221 tgaataaaat gtgcacgttt atcagttact ttcattgcat attcagcaaa ctgttgattt
    20281 aatacttcca acattgttaa gtcctttttt tggcctaatt gtaactgctt taagtaatta
    20341 ttcttttgct ttaaaatacg ttggtattga gctaaatcat ttaagtaaac agcagaaatt
    20401 tggcccaact ccatatctat aaagcgtcgt cttatttgag gagagccttt tacaatattc
    20461 aaatcttctg gcgcaaatag aaccacattg aggtgtccaa tatattgagt tagacgactt
    20521 tgctctaagt gattcacttt gacttgttta ccttttttag ttataaacat tgttaatggc
    20581 atcgtgccgt gtctataact aagctcacct tctattttag cataatcagc attaaaacgt
    20641 atgagttcct tatcattact cgttctatga ctttttgcta aagctaaggt ataaattgat
    20701 tcaagtaaat ttgtctttcc ttgtgcattt tctccaatga ggatattcac gtcaggatga
    20761 catttcaacg taacctcatc atagttacga taattttcta attggagtgt atttaacttc
    20821 attgttcacc ttgatgaatg attaagaaag aaccagcatc ttcaggtaat tctgggatat
    20881 ctatacgatc ttgatgttct aactttttac cgcgacgtgt ttcacgcact ccattaatta
    20941 atacttcaac gtcttgcaag aaccattttg cttgaccacc agattcaata atcccttctg
    21001 tctttagaaa ttgacctaaa ttaatgtctc cttctactac aacttcttga accaaaataa
    21061 tcactccatt tctttgtatg tcattaatat attatacctt tttttgtcac aaatttcatt
    21121 taaaatagag gtagaaaccg tttacatata aataattaat tcttattttt tggcaatact
    21181 gtcattttca attttttaac gctatttaac ggcgtttaac ttttatattc aacttgttat
    21241 tgactattaa aattttaaat ttaaagggca tataagcaca cttattaaat tttggataca
    21301 tttgtcataa atttaattgg cgtcttttat ttgctgttta aaatgctgtc aaatttat
//
LOCUS       DAKSQE010000038        21053 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.038, whole genome shotgun sequence.
ACCESSION   DAKSQE010000038 DAKSQE010000000
VERSION     DAKSQE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21053
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.038"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(209..769)
                     /locus_tag="O0V33_001227"
     CDS             complement(209..769)
                     /locus_tag="O0V33_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCX8975869.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(936..2819)
                     /gene="sasF"
                     /locus_tag="O0V33_001228"
     CDS             complement(936..2819)
                     /gene="sasF"
                     /locus_tag="O0V33_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HCX8975870.1"
                     /translation="MAKYRGKPFQLYVKLSCSTMLATSIILTNILPYDAQAASEKDTE
                     ISTEILSKQDLLDKVDKANRQIEQLKQLSASSKAQLNEAKTASQIDEIIKRANELDSK
                     DNKGFQIEMNGQSDIDSKLDQLLKDLNEVSSKVDRGQQSGEDDLNAMKNDMSQTATTK
                     HGEKDDKNDEAMVDKALEDLEHLNQQIRKSKDTSEDQAVSTTDNYHEVAKTPNNDGSG
                     HVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTYHKLNTLQY
                     LDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYATQRILESIF
                     NKDEADKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQDKSLLISQ
                     ILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKAILNNAKDK
                     KQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLLNLQKRLNQ
                     TKKDIDYVLSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSNLLDSIPDIPTPKPEK
                     TLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIWLSRRNKLK
                     NKA"
     gene            complement(3061..4419)
                     /gene="gtfB"
                     /locus_tag="O0V33_001229"
     CDS             complement(3061..4419)
                     /gene="gtfB"
                     /locus_tag="O0V33_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HCX8975871.1"
                     /translation="MIKLFDYFNDHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFVLFFIKQLEITEHHFVINSLALPFSVLYNLPSNGSDVL
                     VWQEQCDGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQAL
                     ES"
     gene            complement(4412..5920)
                     /gene="gtfA"
                     /locus_tag="O0V33_001230"
     CDS             complement(4412..5920)
                     /gene="gtfA"
                     /locus_tag="O0V33_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HCX8975872.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFEDQEVIWLYQYFSDISIAPTTYTLDDIERELGHDISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDIVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNEDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALLYPTLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYTD
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVVTKLAHAIVMYFNNGPQTPHDISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            complement(5938..8328)
                     /gene="secA2"
                     /locus_tag="O0V33_001231"
     CDS             complement(5938..8328)
                     /gene="secA2"
                     /locus_tag="O0V33_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HCX8975873.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDALKQKTI
                     EFKERLVSGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEKAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGIEVSTDKSVMATITFQNLFKLFGSFSGMTATGKLGESEFFDLYSKI
                     VVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSSLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENRDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQVALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAS
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            complement(8318..9277)
                     /gene="asp3"
                     /locus_tag="O0V33_001232"
     CDS             complement(8318..9277)
                     /gene="asp3"
                     /locus_tag="O0V33_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HCX8975874.1"
                     /translation="MLKNKTFKVTWNYISQTTFMNGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQDDLEYKSMRVTKIG
                     DDQYGNDRINVIIAEPSDAYPTISNDFLKPFGHVWLVERWMDDNIEENIKQLKNDLQS
                     QDTLKAINLISYGSKSNVFATYVAQHLDCKVYRTSHEDDDLKDWFKEHVLGNHELKDT
                     NVEVYFKEEQDNHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            complement(9255..10823)
                     /gene="asp2"
                     /locus_tag="O0V33_001233"
     CDS             complement(9255..10823)
                     /gene="asp2"
                     /locus_tag="O0V33_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HCX8975875.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQTISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIVLT
                     DDMLDSPLEIPTKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIEETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPHAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            complement(10813..12366)
                     /gene="asp1"
                     /locus_tag="O0V33_001234"
     CDS             complement(10813..12366)
                     /gene="asp1"
                     /locus_tag="O0V33_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HCX8975876.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQTQNKTEFDDMISLVGMHLEN
                     NLDYQLIVLNHAPNIRTFLHRYDLYETKYWSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DDQGEASYQEYLTINGDCVLYENFKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKSLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDVD
                     LQQMMQRLVDYIAQEDNYRLILLTRHQNDIPMWLRECITSVNEEYQAKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRIINSVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            complement(12377..13588)
                     /gene="secY2"
                     /locus_tag="O0V33_001235"
     CDS             complement(12377..13588)
                     /gene="secY2"
                     /locus_tag="O0V33_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HCX8975877.1"
                     /translation="MLKLLQQYEYKIIYKRILYTCFILFIYILGTNISIVSYNDMQVK
                     HESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILMLISYRNMDKYMKQTSLEKHYK
                     ERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILILVTGTMLLVWLADKNSRYGIA
                     GPMPIVMVSIIKSMMHQKMEYIDASHIVIALLIILVIITLFILLFIELVEVRIPYIDL
                     MNVSATNMKSYLSWKINPAGSITLMMSISAFVFFKSGIHFILSMFNKDVSDEMPMMTF
                     DSPIGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGVKPGKDTERYLNYQA
                     RRVCWFGSALVTIIIGIPLYFTLFVPHLSTEIYFSVQLIVLVYISINIAETIRTYLYL
                     DKYKPFLNQYW"
     gene            complement(13717..20730)
                     /gene="sasA"
                     /locus_tag="O0V33_001236"
                     /pseudo
     CDS             complement(13717..20730)
                     /gene="sasA"
                     /locus_tag="O0V33_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044594.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
BASE COUNT         6145 a         4032 c         3338 g         7538 t
ORIGIN      
        1 atttctgttc ataggtccga tttcttattc aattaaaccc gaggcattca gttcgaacgc
       61 ctcgggtcat tttatataaa tatattattt tatgttcaaa tgttcctcat catatccgtt
      121 tcaattgcca tctcacacat tttataaata tgagcaaatg tacttattct caaacattac
      181 tgccgagctt taattgacgt tatattaact ataaactact tttccatgac tctacggatt
      241 caatgtcaca tgagcgtgat aaaatttgtt caataataaa gtcatgttta tcatctgatc
      301 tatcaccaac agcatcttct aaaacagtaa tataatagtc tttatctaca ctttctaatg
      361 ccgtgctcaa tacagctcca cttgtagaga cacccgttaa tactaaatga ttaatatcat
      421 ttgcacgtaa ataaacttcc aagtaactac ctgtaaatgc gctaaagcgt cgcttagaaa
      481 taatcggctc atcttctagt ggtgctaagt cttcaagtat tcgtgtagat gcatctgctt
      541 cagtaatcgc atatccttga gctttaattg ttgaaaacac tttattactc gaggagacat
      601 cattaaaatg cttatctaac actaaacgta tgaaaatgac tggtattcga tgttgtcttg
      661 ctgcttcaat tgctctctga ttcgctttaa taatattttt tattctaggt acactactcg
      721 ctataccttc ttgcatatcc aaaactaata gcgccgtttt tcgagacatc ttcattctcc
      781 tttacttgtg tagttctaag tcgttaaatt cattataacg ttaaaatgat ggacaatcta
      841 ttcattgcat tttgcatata cttcacaata aattaagggg aaataagacg tcttatatac
      901 ttaaaaaata tatagatgca cttcccccaa tataattatg ctttattttt caacttattg
      961 cgtcgtgata accaaatcat tagtacaccc attgcaccaa caattacaga tatcggcaac
     1021 caatgttctt ttatcgtttc ccccgcttta ggcaaagata cattaccatc agcatttaat
     1081 aatccactta acaatccatt ccctttacca agtgttaacg tcttttctgg ctttggtgtg
     1141 ggtatatctg gaatactgtc taataaattt gatccttgat tcattaaatt tgctaactta
     1201 tttaaatctg tcgttttccc atttttattc aatctatcta gtaaacttgg acgatttact
     1261 attggtgata aaacatagtc tatatctttt ttcgtttgat tgagtctctt ttgtaaattc
     1321 aataaatcat ccgctttacc attcaatgcc gatttaacta aattaaaaat tttattttga
     1381 tctgtttcta ttttagtaat taaatctgcc agtaattttg ccttttgtct ttctatacgt
     1441 gttgctaaaa tcgtttcaat tgcttgtttt ttatctttgg cattattcaa aattgctttt
     1501 aatatatcat ctgaagacgt gtcgccagtt gatgcaaaat gtttcttcaa ttggtcaacg
     1561 atttggcgat ttgataatcc tttattcgtc caatctttag ccaatttatc tgcttcagct
     1621 tttcctaatt tcgtttgtaa aatttgagaa atcaatagcg acttatcttg tgattgatca
     1681 atcaatgacg ttaataaatc atcactcgtt gtcagagata gttgatcaat atgacgagta
     1741 atttgatctg caatttgttg atctgtttta ccatcaacac gtatatcttt tagaatttta
     1801 tctgcctcgt ctttattaaa tatactttct aaaatgcgtt gtgtagcata ctttttatca
     1861 tcagtacgtg caagttcttc caaaataata tttcgttgac tttttatacg ctctttcgtc
     1921 ttatttactt cgctcattaa gtctgatttt tgatttttag gaagttgcgt atttgcaata
     1981 cgttgatcta aatattgtaa tgtattcagt ttatgatatg tgtaatgttg cgttgaggca
     2041 ttacttttag ccaatttttc aatcatagca tgattaattt tatcgcttcc ttgtaattta
     2101 tcagtgagtc gattactatg gctttgattc tcttcatttg aaagaaattt atttaacaca
     2161 acatgtccag aaccatcatt atttggcgtt ttagctactt catgataatt atctgttgta
     2221 gacactgctt gatcttccga tgtatctttc gatttgcgta tttgctgatt caaatgctct
     2281 aagtcttcta acgccttatc taccattgct tcatcatttt tatcatcttt ttctccatgt
     2341 tttgttgtag ccgtttgtga catatcattt ttcattgcat taagatcgtc ctcgccactt
     2401 tgttgacccc tatcaacctt tgaagaaacc tcatttaaat ctttaagcaa ttgatctaat
     2461 ttactgtcta tatcactttg accgttcatt tcaatttgaa atcctttatt gtctttgcta
     2521 tccaactcat tagctcgttt aatgatttca tctatttgcg atgctgtttt cgcttcattt
     2581 agttgtgctt tagatgaagc cgataactgt tttaattgct caatttgacg atttgctttg
     2641 tcaactttgt ctaataaatc ttgcttagat aatatctctg ttgaaatttc agtatccttt
     2701 tcagatgcag cttgggcatc gtacggcaag atattcgtta aaatgatact agtcgccaac
     2761 attgtcgaac acgataactt tacatataat tggaacggtt tccctcgata tttagccatc
     2821 aacatactcc tttctcactt acttccttaa aagaattaca tactattata tacctgttta
     2881 caaggaattt acacttatct atctagttat tgttgttagt aattatccac ttattgctta
     2941 gcttatattt aagtaaacaa aaaaagcatg acgtaatatc atattgtcca tgtcgctaac
     3001 atcatattac gtcaaatctt ttatattaaa tgatgcttta tttcagactg ctttttcttt
     3061 ttagctttcg agcgcctgtt taaaaacttg ctcgaattgt tcacgcgaga tttcgtgtgc
     3121 atgtgctttt tgtgctaata aagcatctcg aaactgttgt tgatctttca aactttctaa
     3181 catttgtatt aattggtctt tactttccat tgttatctca tcattatgct caaataagtg
     3241 ctctgatagc gttactttag catggtgtgc tgtttgacga taacctaaaa tcaacaactc
     3301 atagtcaaac gcttgttcca ccgcatttaa aatttcatta ccctcattga tatcaagata
     3361 aatatcacat aactggtata gttcatttac cctgtctata ttaatagatg ggtataagtg
     3421 cacattagca tattgatcaa gttgcattag cttatcagac atctctgtaa tagcagcgat
     3481 gtgaaactta aaatctggta aagtttcaac caataccttg atgttacgaa gttgatccga
     3541 gttagttaat attacaattt ctttagtata tctattacga ctacgatagt tatatagata
     3601 tccgccttgt aaaatacgag attgaacctt tgcgtctgct atattgagca tcgtttcata
     3661 ttcgttttta tctggaataa taatattaca atgtcgtttc atatcacctt tacacatcaa
     3721 ttgcatattt cccgggacat taccatcgca ctgttcttgc cataccaaaa catcactacc
     3781 atttgatggc aaattatata acactgaaaa tggtagggct agtgagttaa taacgaaatg
     3841 atgttccgta atttcaagtt gcttgataaa aaataatacg aatgcgagct ttgaagggaa
     3901 aaagtaagac ttcccttgcc aatccaatat gacatcagat gttacaaaat tttcataaat
     3961 cacttcttta ccttctgctg tcatatattt cttcaagatc gctttacgat ttaaatcgta
     4021 aacagtttgt gcaaatttaa taccattctt agaataataa tcgacaaatc ggacacgttg
     4081 ttggtcatca aaccattcga cacgactaac aattctaggg cgctctccac tttgataaaa
     4141 tattttgcct cgtagacgtc ccatatcatt aattgtagcc gaattgttgt tacctttaat
     4201 ttcccaaaaa gctggtacag taacctgatt aaaaaatcgt ggtttcatat tttctgtatt
     4261 atgattatct gcaaaaaatt gatacggtga tataacatcg tccggtaaaa aaccattgtc
     4321 attgagtaca attgttaaat cttcctccaa cttactggct ttaaaagact catataactt
     4381 tcgtgaatga tcgttaaagt aatcaaataa tttaatcatg cagcacctct tgaactaatg
     4441 tttcccattt taaaataata tcttgagtca taaattgctg tgccacttca taagagatgt
     4501 catgtggtgt ctggggacca ttgttaaaat acattacaat ggcatgagct agttttgtga
     4561 caacatcatc cacactatct tcatcggtat caaaaggtac caagtagcca ttttccccat
     4621 ctcgaataaa ggttgggtta ccataattca catttaatcc aatcatacct agtcctgagc
     4681 ctaccgcttc cattagtgtt aacccaaaac cttcgctagt tgatgcagaa agaaataact
     4741 cataatcagt ataaatttca tcaagtttaa catgacctag taaacgaata taatcttgcg
     4801 cgcggtgtgt atcaataatt ttacgcagtc gcgtcttctc gctaccttct ccataaatat
     4861 caaatgttaa ttctggcact tgtcgtttag ccacgataac tgctttgaca agccaatcaa
     4921 tatgtttctc atttgctaaa cgagatgcac taatcatcgc atatggcttt cttgataatg
     4981 ttggatataa taatgcatca atgcttccca caggaatagt ataaacacgt gggcgataac
     5041 cttgatattg ctcaaattgt cgacaaacca tatgattttg aatatctgtt gctgtaataa
     5101 agaaatcaat gtatttagct tttgaaaatt gatattcata ataattgttc catagtatat
     5161 gctgctcgct catcatatta ttactataat gatcagcatg aatcacaaca ccaactttac
     5221 tatcaccttt atgctgcaaa acagcctgac caatatcaga agcgcggtct aatatgacaa
     5281 tatcgtctcg ggttaaattc aatcgttgta aaaagtatgc aataaattcc gttttgttat
     5341 ataacaccgc atcttcaaac acatatatag agctgtctcc atcaatatat tcgttataag
     5401 cgatggaacc atcttcatta tagaattgtc gcatgtataa tttcgcttta ttatcagctg
     5461 gtgcataata ctcagaaaat atgcgtgtat aactataaaa atctttacgt actaacatac
     5521 tattaattac aaattctgca cgatccacaa tgtctttttg ttcattttgc agataacatg
     5581 ttacaaatga tgatttccca ttaaaatata ggcggactat cttaccattt ctttctctaa
     5641 aactaatatc atgaccaagc tcacgttcaa tgtcatctaa cgtgtacgtt gttggtgcta
     5701 tagaaatatc actaaaatac tgatacaacc aaataacttc ttgatcttca aacccaatgt
     5761 tttgcgttaa tgtctgtatg ttctctgact gtataaaatc taaaaacaca aatttagtgt
     5821 cttgatttgt acgtctcaat aatttagcac ggtaagcttg tgcatattca acaccgctac
     5881 ttgcccagcc tataccaaag tttatattat atattgtcat gcgctacccc ttttcattta
     5941 tggaaaatgt ataactggca taccttcttt atcaaatgta atcatgcttt gacaaatatt
     6001 tttcaccatt ctttttttga tatttcgtgt cataacttca aatgaatcta atgcaactct
     6061 atggtattca aaaatagcgt tacgttgccc attttgtcgt tgattaacgc ttgcttttaa
     6121 ttgttgtaaa tagtcgactt gttctaacca gcatgaatca attgctttca aaaagacttt
     6181 ttgaacgaaa atattataat aatatgcact ttgcatgttt ttacgattca aagctacttg
     6241 cttttcaaat tgctctaata aaaatgttac tactgcttgc ttatctttaa aattaacaca
     6301 agccacatct ttattaaatt ggaaacttaa attttgataa atatactcga caacacgcga
     6361 ttttgttagc accttttcct catttacaaa catttcaaac acatctttag ctaacgcttt
     6421 aaaatctcga ttctcagcat catctatttc taaaactcga ttgcgttcct cgtatacaag
     6481 atctcgctgt atactaatgc ttttttcaaa ttcattagcc atttcacgag ctttaacccc
     6541 ttgttcttcc gagatacgct gcgctttaac tacaatttgc ttaactttgc gattaaacaa
     6601 actactttgc gataatcgtt gtgcatctaa tgaatataat tgattatttt ccgctaaatt
     6661 actatcgctc catcgcttaa ctaaataatc atctagtgaa atatatatac aagatgatcc
     6721 cggatcccct tgtctaccag aacgaccacg taattgcctg tctacacggc tattttccat
     6781 atgttcatga ataataactg ctaatccacc taatgcttcg acaccttcac caagtttaat
     6841 atctgtgcct cgacctgcca tactagtcgc aacagtcatg gaaccaattt gccctgcttc
     6901 agctatcatc tgcgcttctt ttgcaacatt ttgcgcaatg agtaaattat taggaatatc
     6961 catttggaat aatactttcg aaaagtattc agccgcttca gcagttctcg ttatgagtaa
     7021 aaccggtcgc cccgtttcat gaagttcaac tatatcatga atcatcgcga tgtttttctc
     7081 atcaactgaa cgaaacactt tatctggttc atcgatacgt tgaatcgctt tatcagttgg
     7141 tacttgtacg actatttttg aatacaaatc aaagaactct gattcgccta attttcctgt
     7201 agctgtcata cctgaaaatg atccaaaaag tttaaataaa ttctggaaag taattgttgc
     7261 cataacactt ttatctgttg aaacctcgat accttctttc gcttcaatag cttggtgaag
     7321 tccagcttgc aacttagttc ccggtaacat acgacctgta atacggtcaa ttaaaacaat
     7381 atcaccatta tatacgaaat aatcgacatt agattcaaac aaatattgcg cgcgcagtgc
     7441 tagattaata ttacgcacta agaccatcgc tttttcgcta tataaatctt caacattaaa
     7501 gtatgattgt gccgcttcaa taccttgatt taacagccat atttcttttt tggtcttctt
     7561 cattttaaaa tgcacgtctt caatcaatgt atctacaaac tctttcacaa tatgaaatag
     7621 atttgattgt aatcttggtg cgcctgaaat aactaatggt gtttgagcag catctaaaat
     7681 gattgagtcc acttcatcaa taataccgta atttaattgt ggtaaaaatt ttccttccgc
     7741 actatctgcc aaattatcaa ttaaataatc aaaaccgaga cgtccattag ttgtatatat
     7801 aatatcatgt tcatatatat tacgtttttc ccctttttga tactcataat ccacaatatc
     7861 aacaaaacct aatgaagcag ttaaacctaa ccattcatat aatggttgca tctcttcaaa
     7921 atcacgcttc gctaaataat cattcgttgt aattaaatat gttccttttc ccgaaagtgc
     7981 atttaaatat aaaggcatcg ttgctgttaa tgttttacct tcgcctgttt gcatctccgc
     8041 aatgttacct tcatgcaata caatcgctcc gattaactga acttctttag gatacatacc
     8101 taatactctc cagctcgctt cacgtgccac tgcataagct tcaggtaaca atgtgtctag
     8161 tgtatcaact cctgatacta aacgttcttt aaattctatt gtcttttgtt ttaacgcatc
     8221 atcagaatat gatttaactt catcgctcca tgtattgatg cgtttcacta tttttctaat
     8281 cgactttagt cttaattcgt ttatcgtaac atctagttta tgtttcattt acttccccac
     8341 cattcagttt cgatacatct aagtaatcta aaaatcgtac tggattcatt aaacgtgaca
     8401 tataatttag atggttgtct tgctcttctt taaaataaac ctcgacattt gtatctttta
     8461 gttcatgatt tcctaggaca tgttctttaa accaatcttt taaatcatca tcttcatggc
     8521 ttgtacgata cactttgcaa tctaaatgct gagcgacata agttgcaaaa acatttgact
     8581 ttgacccata actaatcaaa ttaatagcct ttagggtatc ttgactttgc aaatcattct
     8641 ttagttgctt aatattttcc tcgatattgt cgtccatcca acgttcaacg agccaaacat
     8701 gaccaaacgg tttcaaaaaa tcattcgaaa tagttggata ggcgtcagat ggttctgcaa
     8761 taatgacatt gatcctatca tttccatatt ggtcatcacc tatcttcgtc acccgcatgc
     8821 ttttatactc taaatcatct tgatgcgtca tctctgtaat tgttaaacat ctaaatataa
     8881 gactcgtcga tgctgcattc atcattttta ttttataagc ataggcttca tcaggatatt
     8941 gaatcgtaat actatttgac tttacaatct cagtacttag ttttgtgcca tttttattat
     9001 aaaaaatgat gataaaatac actgaaccag caggcgttgc atcaaaatca aaatgcaatt
     9061 tataatgctg tcctctacgc aaaattggta aacttggcgc acttttatat tttgaaaatt
     9121 gttttaacat caaccactca tgaatcggta atccagaggg catcaaagga tttataaaag
     9181 tcacttcacc atttgaaaat gatacttttg agccattcat aaatgtagtt tgtgaaatat
     9241 aattccaagt aactttaaat gttttgtttt tcagcatgtt gaactctccc aaacttgtct
     9301 tccaaaataa tgttgtaaaa attaacaaac caacttgcaa tggtaggtga atcatcatta
     9361 tgtcgcccag gaatactgcg attcatcact cttgcttggt gtgctgtcaa tacaggtagt
     9421 agctcttgaa atgcatgtgg atcataatca tcatgttgca tatatgctat ggcaaaaaca
     9481 gtttgtgaca atgatttctt ttgaaatgtt tgccaaaatt tttgatttaa tgcctgtatc
     9541 gacgcttgag atgtatcacc ttcattagac accaggacgt ctaatgctgt accgaactct
     9601 tctggtctaa gtaatcgcat atgttcagca atcgttccaa tattaacaag tggtttacca
     9661 acaataattg cctgaggatt taactgagca ccataataca aggcaccaaa tgaacccata
     9721 gataaaccag ataatattaa ttcatgtgat ttaaaattca acttttctaa tgtctcttca
     9781 ataacattga taataccttg ttcatattca gatgaaccga tataaaagct accaccttca
     9841 acacgaggat cgccgataag taaaaacggt gcattcatac gtttcatcat ataataccct
     9901 tcgaaacctt ccgctgttcg ataaccacta aaatatacgt ttagtggtgg tttcatatca
     9961 ccagggtgga aataataaat aaattcctgt cgttgactat ctacgaaacg actaccacca
    10021 agtaaaaatt gacccatgtc taatctagac catcgtttat gtataggtcc taaatgtacc
    10081 gtcccgttcc cacgcgcctt aacagttaca cttatataag catcaaatgg tttcgtaggt
    10141 atctctaaag gactgtctaa catatcatca gtcaatacga tttgttcaat taatgcacca
    10201 tcagcgccag tctgaatcaa tctaaatgta tattgcaact cgaccgcacc atcaatatca
    10261 aattctggcc atatttgaat gactttatct ttatcgtaaa cgagattatt ttgccaagat
    10321 gcgataggtt taaattcttt tccaaattct ccactcaacg tgagctctga attaccttgg
    10381 taaacgacat ctcctttaaa attcggatga acaagtgcta acttaggaga aaccttatct
    10441 ccatactgtc ctgagaagct aactgcctct aatttattat tacgttcttc aatgttccgg
    10501 taatgtaatg gttgaacaac gtatttttgg acattttcgt cttgttcata ttcaacagac
    10561 caaaatgatt catcaacata cgtattgtat ggttcgctta tcgtttgtaa taaattcgtt
    10621 aatgtctccg agtatggtgc ttgaatatag ataaaatcaa agcgcccttc tgcttcaaca
    10681 atcgcttcaa tagcctctac ataaccacta tcaaattcaa acaatccaat atcgaagtaa
    10741 tcccaactca cacctttttt gtgctggaaa ataggttcta aatcgtctcc tccaatttgc
    10801 aaaactctaa atttacgtgg catcattttc accttctatt aactcatcga gctgattaat
    10861 aatattctta gaagcatatg catctattaa ttttaaagaa taggcgtacg cataattcca
    10921 atttttcaaa taaaataaat aataatttaa cgcatcatct aattcatcaa ctgaatttat
    10981 aatacggcca ttgtcataat cagagacgta atctgtttgt tggccattaa tttgtggaat
    11041 cccagcgcta attgcactaa tttgtaaata caaatcaggt tcttttgaca tatctatcac
    11101 aagtcgcaac gtccgtaatg cttctacaac atcatgttca gcatgtatcg tcttaacagc
    11161 aatgatgtca tcttgatctt caggtgtcat taatgctgaa acattaacat ccgcattctg
    11221 tttagcttgg tattcctcat ttaccgacgt aatacattca cgaagccaca tcggtatgtc
    11281 attttggtgg cgcgttaata aaattaaacg gtaattatct tcttgtgcga tataatccac
    11341 aagccgttgc atcatttgtt gcaaatcaac gtcactcata ccgtctatcc atacacctat
    11401 aaatgtttcc atcaattgac tacttatatt aggtgattgt ctcgtttcaa atggcgtgat
    11461 tcgaatcatt gtattctctc gctgatactt ctcttgatga ctaattaaat cacgttctag
    11521 ttgcacacta tcaacaatta aatgagcatt ctttacgata gattgatatt cctcatctga
    11581 cacagtttca tttctatttt taaaaaatga ataacttaat gatttcgccg gaatatgatt
    11641 ggctatttgt cgattgtgcc tagcatctga agccacaatc acatgatcat cttcatgtat
    11701 ttgttgtgca atcattgctt gaaatttttc ttcaattagt tgagccatat tgttatattc
    11761 tgtttgttga tagtgatgtt gatatctttt tgaaacagtg actcgtccat ttttaaagtt
    11821 ttcataaagc acacaatctc cattaatcgt taaatattct tggtaagaag cctctccctg
    11881 atcatcaaaa taacgtatag ctgataaata acctctgtca tcaaaaatat aacgccgttg
    11941 taactgatct ctttcaaatt cttcaaacca aattgaatat ccttcttgac taaaataaat
    12001 atttgtatag gtctgttcac tcgtcacaca ttttaataaa tacggcgtgt acacaaactc
    12061 aacatcatcc ggccatttta agtgatgata attaatcgct tgtggcgcat ggtgactgaa
    12121 tccttgaatt tcatcaaaca cagaccaata ctttgtttca tacaaatcat atcgatgtaa
    12181 aaatgttctt atattcggcg catgattgag aacaatcagt tgataatcta aattattttc
    12241 aaggtgcatc cccactaaac taatcatatc gtcaaattcc gtcttatttt gcgtctgata
    12301 atacggcaca gtcgtgtctt gccaccatcg ttggtcatcg taccaagctg gaataaagta
    12361 tttcataatt acctccttac caatactggt ttaaaaatgg cttatattta tcaagatata
    12421 aatatgtacg aattgtttct gcaatattaa tactgatgta aactaataca atcagttgta
    12481 ctgagaaata aatttcagta gataaatgcg gtacaaacaa tgtgaaataa agcggtatac
    12541 caataatgat tgtaactaat gccgatccaa accaacatac gcgtcgtgct tgataattta
    12601 aataacgttc tgtatcctta ccaggtttaa ctcctgaaaa ataattgcca ctctttaaga
    12661 aatcttttga tttttgttta gtattgatta aaaatctcga taaaaaataa cccaataaca
    12721 tttgaatcac taaatatact gaaataccaa ttggactatc gaatgtcatc attggcattt
    12781 catctgaaac gtctttatta aacatagata aaataaaatg aataccactt ttaaagaaaa
    12841 caaaagctga aatgctcatc attaatgtaa tactgcctgc aggattaatc ttccaagaca
    12901 aataagattt catatttgta gccgagacat tcatcaaatc aatatacgga attctaactt
    12961 ctactaattc aatgaataat aggataaaca atgtgattat cacaaggatg attaacaacg
    13021 caattacaat atgacttgca tctatatatt ccattttttg atgcatcatt gatttaataa
    13081 tgctaaccat tacaatcggc attggtcctg cgatgccgta acgactattt ttgtcagcta
    13141 accaaactaa taacatcgtt ccagtaacca aaatcaatat tgttaagtaa atattgtctt
    13201 gatgaacacg ttctttcgaa acatattcat gaatcacaaa ataactttga ataacactta
    13261 aaattaatgt taagatgcgc tctttataat gcttctcaag acttgtttgt ttcatgtatt
    13321 tatccatatt ccgataagaa atcagcatca agataatcat tgatgttagc cacggaccta
    13381 atcccaaagt aaaaatgttt aaagtattaa cgtctccacc catattagaa atagctattt
    13441 taaaaaacga ctcatgtttt acttgcatat cgttatacga aacgatagaa atatttgtac
    13501 ctaatatata aataaacaag ataaaacatg tgtaaagtat acgtttatat ataattttat
    13561 attcgtattg ttgtaaaagt tttaacatgt tacacctctt ttatatcaaa aacattaaaa
    13621 agactaagga tatttcacta attattaaaa tctgatatag attttttagt gaacaacgcc
    13681 ccagtctttt taattttagc cagctataaa ttcaatttat gcttgagaat catcttgctc
    13741 attttcatct ttctttttct ttctcttcat taaacctaaa ccaactaata atgtcataac
    13801 gccacctagt aatccatttt gttttattga gtcacctgta tctggtaatc tttcttcact
    13861 ttgcgctcgt gtgccattat gtttagtcgc ttcagatgtt gcacttaatg tagactgaga
    13921 ctcactcgtg ctcgttgttg cttcacttgt tgaaatagat gtgctcgtgc tgtgtgtatg
    13981 atgcatactc attgagtctg acggatgcat tgagttagat tctgatgtac tcattgacat
    14041 tgagttagac atacttgttg atgttgagtc agaaatgctt tgtgaaccgg acatagatgc
    14101 actcagcgat tcggatgtgc tcaatgacat tgaattgctc attgacactg attctgagtc
    14161 actcattgat gttgagtcag acatacttgt tgatgttgag tcagaaatgc tttgtgaacc
    14221 ggacatagat gcactcagcg attcggatgt gcttgtcgaa tcggatgtgc ttaatgacgt
    14281 tgatgcgctt gttgacactg attctgagtc actcactgat gttgagtcag acatacttgt
    14341 tgatgttgaa ttgctcattg aaactgagtc tgagtcgctc actgaagtcg aattgtgcgt
    14401 tgaaccagac atacttaatg attcagatgt acttatcgaa gtcgagccac ttgttgatcc
    14461 ggaaatactt tgtgacattg aaatactcgc tgaattagat tcactcattg atgttgagtc
    14521 ggatacgctc gttgaaccag atgtgcttaa ggaaccagac gtacttaatg agtcggatac
    14581 gcttgttgaa cctgaattag acatacttaa cgaacctgat gcgcttgttg aagttgaacc
    14641 acttattgag cctgacgtgc ttaatgacaa tgatgtactt gtcgagtccg atgtgcttgt
    14701 cgatgtcgag tctgagccag atgtactcaa tgattctgag tcactgatag aagttgaatc
    14761 acttgtagat tctgattcta cagcactttg tgaaccactg atacttattg aagtcgaatc
    14821 acttatactg tctcatgttg ataatgacgt cgacaccgac gtgctttgtg atgtcgatgt
    14881 acttgcactt gctgacattg atgatgatat cgatgtgctt aacgaacctg atgcgcttgt
    14941 gcttgttgac tggctttgtg ccatagaatc acttagcgac tctgatgtac tcgttgagct
    15001 cgagtcactt acacttattg aacctgatat tgagtcactt aaacttgtcg atgttgaaac
    15061 tgatacgctt ccgctcattg aatcagatgt tgaaagtgat gtactcgttg aatttgaccc
    15121 actgatgcta gacgaatcgc ttgtagacat tgagtcactt tctgatgcac tgatgctcat
    15181 agagtcaaat tgactattac ttgttgagct tgactgcgaa tcgctcacac ttgttgacgt
    15241 tgattctgat ccactcatac tttgcgagct actcaatgat tttgaatcac ttaatgaatc
    15301 agaagtgcta agacttgtgg aaccacttaa agatattgat ccacttaatg agtcggagtc
    15361 acttgtacta gtagaatcac tcattgatat tgaatcactt agcgagctag actcgcttac
    15421 actttctgaa ccacttaatg atgtcgaagt gctcaatgaa cctgatgtac ttgttgaagt
    15481 cgaaccactt gttgattttg aatcacttaa tgaatcagat tcactcacgc tttctgaact
    15541 tctttgtgac gtcgatacac ttaatgacga cgaatcgctt gtgcttactg aatcactcat
    15601 cgattgtgag ccactcaatg aactagactc gcttacactt tctgattttc ttaatgacgt
    15661 tgagacgctc aatgagccag aatcacttac gcttgttgaa ccactcatcg attttgagtc
    15721 actttcagaa ttagattcac ttacactttc tgaatcattt acagattctg acatactttg
    15781 tgaatcagat atgcttgcgc tcattacttc actagccgat gttgatgtgc ttgtcgaatc
    15841 acttaacgat atagacgcac tcatcgaacc agatgtactc gtacttgttg agtctgatgt
    15901 tgaatcactc acactatcag ataatgacgt tgaatcactc acacttgttg atgtacttgt
    15961 tgaaagcgac atactttgtg aatcacttgt acttgtacgc atcgaagtac tagttgaagc
    16021 tgatgtacta cgagagtcac ttgttgatgt tgatgtactt gccgatcctg atgcacttgt
    16081 acttcttgat gtgctttgtg aatcggattc actgctttgc attgacgctg acgttgaatc
    16141 acttaatgat gttgacgacg aatcggattc acttgcgctt gctgagttac tgagcgatgt
    16201 tgacgtactt gctgatcctg agttgcttat acttgttgag tcactgagcg atgtggatgt
    16261 actttctgat tttgactcac ttgtgcttgt tgatatgctt tgtgaatcgg attcgctttc
    16321 tgaaccactt aatgatgctg atgtgcttgt cgaacctgat tggctcgtac ttctagacat
    16381 acttgttgaa tcgcttagtg acgtagatgt cgatgtggat tcactttggc ttgttgactg
    16441 acttgtatat gatgatgtgc tttgtgaatc ggattcgctc gtgcttgtac ttgttgaggt
    16501 tgaggcactt tggcttgctg agtttgagtc tacaccactt tgattcattg aggcactcag
    16561 tgacaatgat gtactagttg agtcagacaa actttctgat gttgacacac ttgtacttcc
    16621 tgatgttgat tgggacatgc tcaggctcat tgatgttgaa tcggatttac tttcacttga
    16681 tgatgttgag ccggattcac tctcactcgt tgaaccgctt aatgatgtcg atgcacttaa
    16741 tgactctgat tttcttgtac tgattgaatc acttgtcgac attgatgcac ttagtgaatt
    16801 ggacttgcta ccacttgtgg aatcacttaa tgatgttgac agacttgtag agtcagacaa
    16861 acttgtactc gttgatgtgc tcaatgatgt tgagacactc tcgctcttaa ccgttgatgt
    16921 tgatgcgcta ccacttgttg acgttgaaat ggacgtacta ccacttgttg aattgcttaa
    16981 tgatgttgat gtgctaccag attcagaatt actatctgat aaagacgtac tatcgcttgt
    17041 tgaaccactt aaggactctg atgttgattc ggatgtgctt tcactcaatg attcacttaa
    17101 aaaagcagat gcacttttcg actctgaatc acttgtacta tttgattcac ttaatgatat
    17161 agacgtactc tctgaaccag atatcgcttc actgatagaa tcgctcgtcg acgttgagtc
    17221 actcgttgaa tcagactctg atgtacttaa gcttgtggaa tcacttaatg atgttgatgt
    17281 gcgttctgaa ttacttgtac ttagcgatgt cgaagtactc attgaatctg atgtacttgt
    17341 tgagacactt tgtgatattg attcacttga tgcagttgaa gctgatttac tatcactcgt
    17401 tgaatcacta aacgacgttg atatgctcgt tgaatcggat tgacttgtac tcattgaacc
    17461 agatgtactt tgtgattccg acatacgtac tgaactactt gttgatgttg atgtgcttgt
    17521 tgagccagac tgactcgtac ttagtgataa tgacttacta tcagaatcag atgtactttg
    17581 tgaatcactt aatgattctg atgtactacc tgactgagac gtgctcattg aactacttac
    17641 agacattgat ttactgtctg atgcagctaa tgatccactt gtactcatag aatcgcttat
    17701 catttcagaa gtgctcattg agtctgatgt gcttgttgag acactttgtg accctgctac
    17761 acttaatgat ccagatactg aatcacttgt gctcattgaa tcgcttaatg actctgatgt
    17821 actcatcgat ttagaatcgc ttgtacttaa tgatattgat gtactttgtg aatctgattt
    17881 gcttgttgac gcactcactg aattcgaaat gctattagaa atacttatag attctgatgt
    17941 actaactgac tcactcaatg attctgacgt actaattgaa tttgatgtac tattacttac
    18001 cgattgtgac gtactagccg aatcggatgt acttgttgat aagctttgtg acttagacaa
    18061 gcttcctgat gtactcatgc ttactgaatc acttaacgat gttgatgtac gcaatgaatc
    18121 agatgtactt gttgatagac tttcggactt ttcagtactg ctagagttcg aaatagaatc
    18181 gcttaatgat gttgatttac taaccgaacc tgacatgctt gatgatacac tttgtgaatt
    18241 cactaaactt gtacttgttg agcttgatac actattactt tcagatgtgc ttaatgactt
    18301 agatgcactc actgaatcag ataaacttgt acttgttgat ttcgaagtac taactgatgt
    18361 tgaagtcatt attgaaccag atgtgctcgt tgatgcactt tgtgagtcag ctttacttgt
    18421 tgacacactt tgggattgtt gggtacttcc cgatgttgat acggaatcac tcatgctatt
    18481 tcttgttact tcatatttaa aagttgtcgt cgttttgtta ccgctcgcat ctgtagaaac
    18541 gattgtaata gtacttgtac caatgtttgt tggtgtacca ctaatagtat tatttgtgct
    18601 atcgaatgtt aatccagacg gcaatcctgt gactgtattt gttactgcat taccactatt
    18661 gtcctgcgta gcaattttaa tcggtgatat tggagaaaaa acttctgatg attgatctcc
    18721 tattggcgtc actgttggtg ctgtcgtatc cacaacattt attgtaaaag ttgtcgtcga
    18781 tttgttattt gcttggtcag tagatactac tgtcactgtt gattgaccaa tttttgttgg
    18841 tgtcccaatg attgaattcg ttgcactatc ataacttaat ccgcttggta atcctgtaac
    18901 tgtatttgtc acagtcccag taccattatc cgttgtagtc aacacaatag gattcattgt
    18961 tttacccact tcaatggttt gattgcctac agttacagtt ggtgctttta catcagtaaa
    19021 ataatatgtc actgattgtc cagcattcgt catttttaca gttttatttg tatcattata
    19081 agttgatgca tatgaactat cgacagatgt gtagttatat cctttagcag tcaatgcaga
    19141 ttgttgatta tcgattgtca cgacttgatc aacatttcct gaatatgttt ttgggggaat
    19201 aatatcttta cctgttgtta catcaacgta tctcacttgt gtaacagcag attctgtata
    19261 ttcgaatgtt ccaaattgta cttgttgtaa atttgtcgca ccacctgttg aggctgtcat
    19321 tgataatgaa aagttggtcg taccactttt tgcaatccaa tctgaaatat tacgtgtcca
    19381 tgtttgacct gcatacgtga cagtcataac ctttgtatca ccattataag taatatcaaa
    19441 attttggaac gtgttgtttg taggttgaac ttttaacttt gcagcattat cagctgttga
    19501 acttgatgtg tacgttgtcg caacgccagt actatctgtt gtaacaaagg caccaaacgc
    19561 acctccacca gcaacactag atgggtcagc attcgccttt gcagctgaat ttggtttaga
    19621 tgtattgtga tacgtatcca atttgaagcc aaatgcgtta cttaggccac caatacctac
    19681 tgcggcaccg tttaaccctg tttcacctaa tacacctggc gaaaaggcaa agccgatacc
    19741 atctccacca tttccattac cttcgtattt attacctaaa tttacttttc cagaaaaatg
    19801 aaaactctta ttagagtcaa tacgtgttcc taatgtaata gcaccttttt ggctgtatgc
    19861 atcctgcgtt aacgtcacaa taccggtact ttgatcataa gtggcattac ctgacgttgt
    19921 catatattgt ttgaggttat ctttattaac cgtaatcgta ttagcggtta ctgcacttgt
    19981 cgttgcagct gatgcaaagg ttgacatagc taatcgactg aaagttcgaa gtttgacagg
    20041 tgctgtgctg gttgacgttg tgctagtttg ttttaaatgg gttgaagctg gcgctgtgct
    20101 ttgtgaagta gtatttgatg cagtgctttg atttgttgat atgttagttt gttgttctgt
    20161 acttgaagtt gaagctacag atttagtatc agaacttgat gtagtattct ttgaggatgt
    20221 tgattctgat gtagatgtta atttttcttg ttgattgctt gtactattag ttgttgaagt
    20281 gacattttca gacttttcac ttgagactgt gtcactattt gatgtttgtt ccgaactact
    20341 atttttcgtt acacttgtgg aatctgctgt tgatgttgat gcttcgattg tcgttgagtt
    20401 ttgattacct actgtttcac tttgtgtatt taattcagat gttaatggtg catcagaagc
    20461 cgcaaaagct tgctggtcat gcaacatgtt tattgtaaat gccccgccga tcactgccgt
    20521 cgttttcagt ccgtatccag tcattttttt actaatgctt tgattatctt gactcactat
    20581 actatgacta ataaatggta gtcccattat tttgaacatt tctatttctt taataccgga
    20641 ttttacccaa ttttttccag atttataaag tcttaccctt gttttttcgt ttgctaagct
    20701 gtcatgaaat tctttctgtc ttttgctcat gtaataactc cttgtattat ctttacattc
    20761 attagattat aatatatgcc actattcaat ttaatacaac tcttttttca aataaaaaaa
    20821 tacttaattt gtcaaaatcc attttttctc aaaagtatat tctttgatga aatcgctttc
    20881 tttattgtgt aggatttagt ataatttttt aactatattc tgaataacat gtcttattat
    20941 gtttatataa ttttgaaatc agtgcttatc atcttggaca tcttttattt atttggtaaa
    21001 aacttcattt tattaaaatg tatttatcat ccacctttta ttcattaata gtc
//
LOCUS       DAKSQE010000039        20964 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.039, whole genome shotgun sequence.
ACCESSION   DAKSQE010000039 DAKSQE010000000
VERSION     DAKSQE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20964
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.039"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            106..1272
                     /gene="aroC"
                     /locus_tag="O0V33_001237"
     CDS             106..1272
                     /gene="aroC"
                     /locus_tag="O0V33_001237"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCX8975878.1"
                     /translation="MRYLTSGESHGPQLTVIVEGVPANLEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISDEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLEQLDIEIY
                     SRVVEIGGIKDKDFYDSETFKANLDRNDVRVIDDGIAQAMRDKIDEAKNDGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGRIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNTELGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLQQQIADRRQLNVEF"
     gene            1298..2362
                     /locus_tag="O0V33_001238"
     CDS             1298..2362
                     /locus_tag="O0V33_001238"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCX8975879.1"
                     /translation="MKLQTTYPSNNYPIFVEHGAIDHISTYIDQFDQSFILIDEHVNQ
                     YFADKFNDILSYENVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFVAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESATQDIEQHFKDREILQSLKGMDKYIAKGI
                     ETKLDIVVADEKEQGVRKFLNLGHTFGHAVEYYHKIPHGHAVMVGIIYQFIVANALFD
                     SKHDINHYIQYLIQLGYPLDMITDLDFETLYQYMLSDKKNDKQGVQMVLIRQFGDIVV
                     QHVDQLTLQHACEQLKTYFK"
     gene            2372..3670
                     /gene="aroA"
                     /locus_tag="O0V33_001239"
     CDS             2372..3670
                     /gene="aroA"
                     /locus_tag="O0V33_001239"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCX8975880.1"
                     /translation="MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYK
                     PLLGEDCRRTMDIFRLLGVDIKEDEDKLVVNSPGYKAFKTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKSMNANIEGIEDNYTPLIIKPSVIKGIN
                     YKMEVASAQVKSAILFASLFSKEATIIKELDVSRNHTETMFRHFNIPIEAEGLSITTI
                     PEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPIKIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            3677..4921
                     /locus_tag="O0V33_001240"
     CDS             3677..4921
                     /locus_tag="O0V33_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975881.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFIWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIEIGQMEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            4935..5510
                     /locus_tag="O0V33_001241"
     CDS             4935..5510
                     /locus_tag="O0V33_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCX8975882.1"
                     /translation="MSETLNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKHNIKLMNTNEIFDYIANHNGAFDIQIHFT
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDD"
     gene            5500..6087
                     /locus_tag="O0V33_001242"
     CDS             5500..6087
                     /locus_tag="O0V33_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HCX8975883.1"
                     /translation="MTINKLWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     QFKIFVPDSPTAILFLVISISLILIQKQNSIIDALAFVTLFKYGIWAVIMNILFIIEQ
                     GDITVNGLVLMFSHSIMAVQAIYFYPRFKRSMIGISVAMTWVFLNDYIDYFHLQFPYY
                     DFITTHVWQIGVLSCCLSVFGLLLYIELNKLLKCK"
     gene            6159..6839
                     /locus_tag="O0V33_001243"
     CDS             6159..6839
                     /locus_tag="O0V33_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCX8975884.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDH
                     QGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLF
                     SIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRI
                     ILIARSSD"
     gene            7175..7492
                     /locus_tag="O0V33_001244"
     CDS             7175..7492
                     /locus_tag="O0V33_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCX8975885.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     KRK"
     gene            7736..8878
                     /gene="bshA"
                     /locus_tag="O0V33_001245"
     CDS             7736..8878
                     /gene="bshA"
                     /locus_tag="O0V33_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HCX8975886.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVREKIPSKLILLGDGPELVPMRQKTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDSDSASDYAIRLLE
                     DKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
     gene            8883..10085
                     /locus_tag="O0V33_001246"
     CDS             8883..10085
                     /locus_tag="O0V33_001246"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCX8975887.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTANKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIATETFEAMRTQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKS
                     LKAFNYMPYFEHLDMNQINVTEPIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTNLIKNVMVAADVLKANDIQGHEPLIVNLQTID
                     ETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDLLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            10072..11043
                     /locus_tag="O0V33_001247"
     CDS             10072..11043
                     /locus_tag="O0V33_001247"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCX8975888.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     DFDESIRYRATSIQLHDKNKLDRYQFLERLLQEIEKRYNQFLTLPFSEIREEYIAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            11067..13760
                     /locus_tag="O0V33_001248"
     CDS             11067..13760
                     /locus_tag="O0V33_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase DinG"
                     /protein_id="HCX8975889.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAEEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIR
                     LYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFDAFKQLFNKDVHFNTFEVNTSL
                     QSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQ
                     DMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVM
                     IAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRNENDRGIIVSFD
                     DRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK"
     gene            14082..15374
                     /gene="asnS"
                     /locus_tag="O0V33_001249"
     CDS             14082..15374
                     /gene="asnS"
                     /locus_tag="O0V33_001249"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCX8975890.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKMLERDTSKLEKVATPFPR
                     ISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
     gene            15703..16389
                     /locus_tag="O0V33_001250"
     CDS             15703..16389
                     /locus_tag="O0V33_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCX8975891.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKEQNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDTAIIQ
                     AALDEANSLNKLSFKYMDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
     gene            16379..17038
                     /gene="nth"
                     /locus_tag="O0V33_001251"
     CDS             16379..17038
                     /gene="nth"
                     /locus_tag="O0V33_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCX8975892.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            17043..17384
                     /locus_tag="O0V33_001252"
     CDS             17043..17384
                     /locus_tag="O0V33_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975893.1"
                     /translation="MTNITKEVFDNLEQKIDVFAKNKTLGSSEAKPYLDEYHSKIIDY
                     FKQVNDITGNIDFDNLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
     gene            complement(17934..20084)
                     /locus_tag="O0V33_001253"
     CDS             complement(17934..20084)
                     /locus_tag="O0V33_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCX8975894.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTAEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQS"
     gene            complement(20081..20758)
                     /gene="recU"
                     /locus_tag="O0V33_001254"
     CDS             complement(20081..20758)
                     /gene="recU"
                     /locus_tag="O0V33_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCX8975895.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNAFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEIFWKRYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLIL
                     DESEDRV"
BASE COUNT         7495 a         2942 c         3775 g         6752 t
ORIGIN      
        1 tcttaattga gcttgaaaac aaacatttat gaatgcacaa tgaatatgat aagattaaca
       61 acatattata atgttatcgt ggaagtatga aaggagcgag tgtgtatgag atacctaaca
      121 tcaggagaat cacatggacc tcaattaaca gttattgttg aaggtgtacc tgcaaattta
      181 gaaattaagg ttgaggatat taataaagaa atgtttaagc gtcaaggcgg ttacggacgt
      241 ggacgtcgta tgcaaattga aaaagataca gtggagattg tttcgggtgt aagaaatggt
      301 tatacattag gtagccctat tacaatggtt gttactaatg atgattttac acattggcga
      361 aaaattatgg gcgctgcgcc aataagcgat gaagaacgag aaaatatgaa acgtacaatt
      421 acgaagccaa gaccgggaca tgcagattta gttggcggta tgaaatataa tcatcgtgac
      481 ttacgaaatg tattagaacg ttcatctgcc agagaaacag cagcacgtgt agcggtcggt
      541 gcactatgca aagttttatt agaacaatta gatatcgaaa tatacagtcg tgttgttgag
      601 ataggtggca ttaaagataa agatttttat gattcagaaa catttaaagc aaaccttgat
      661 cgaaatgacg tccgtgtaat tgatgatggc atcgcacaag caatgcgcga taaaattgat
      721 gaagcgaaaa acgatggtga ttcaataggg ggcgtagttc aagttgtagt tgaaaatatg
      781 cctgttggtg taggtagtta tgtacattat gatcgtaaat tagatggaag aatagcacag
      841 ggtgttgtta gtattaatgc atttaaaggt gtaagttttg gagaaggatt taaagcagct
      901 gaaaagcctg gtagcgaaat tcaagacgaa attctctaca atactgaatt gggctattat
      961 cgtgggtcaa atcacttagg tggtttagaa ggcggtatgt caaatggaat gccaattatt
     1021 gtaaatggtg taatgaaacc tattcctacg ttatataaac cattaaattc agttgatata
     1081 aatactaaag aagactttaa ggcaacaatt gaacgttctg atagttgtgc tgtacctgca
     1141 gcaagtatag tgtgtgaaca cgttgttgca tttgaaatag cgaaagcatt acttgaagag
     1201 tttcaatcaa accatattga acaacttcaa caacaaattg ctgaccgcag acaattaaat
     1261 gttgagtttt aacaacgaga acaattgagg tgtgatcatg aaattacaaa caacctatcc
     1321 ttcaaacaat tatccaatat ttgttgaaca tggtgcaatt gaccatatta gcacgtatat
     1381 tgatcagttt gatcaaagtt ttatattaat tgacgagcat gtaaatcaat attttgctga
     1441 taaatttaat gatattttat catatgaaaa tgtgcataaa gtcattattc cagctggtga
     1501 aaagacgaaa acatttgagc aatatcaaga aacattagaa tacattttgt ctcatcatgt
     1561 aacacgtaat acagcgatta tagctgttgg tggtggtgcg acaggagatt ttgcaggatt
     1621 tgtagcagca acactattaa gaggtgtcca ttttatacaa gttcccacaa cgattttggc
     1681 gcatgattct agtgttggcg gtaaagtggg tattaactca aagcaaggta aaaaccttat
     1741 cggtgcattt tatcgtccaa ctgctgttat ttatgattta gactttttaa agacgttacc
     1801 atttgagcaa atattaagtg gctatgcaga agtttataag catgcgttat tgaatggtga
     1861 atcagcgacg caagatatcg aacagcactt taaagataga gagatattac agtcattaaa
     1921 aggtatggat aaatatattg ctaaaggtat tgaaacgaag ctagatattg ttgttgcaga
     1981 tgaaaaagaa caaggtgtac gtaaattttt aaatttaggt catacatttg ggcatgctgt
     2041 tgaatactat cataaaatac cacatggtca tgcagtgatg gttggcatta tctatcaatt
     2101 tatagttgcg aatgctttat ttgattctaa gcatgatatt aatcattata ttcaatattt
     2161 aatacaactt ggctatcctt tagacatgat aactgacttg gattttgaaa cgttatacca
     2221 atatatgcta agtgataaaa agaatgataa gcaaggtgta caaatggtct tgattagaca
     2281 atttggagat atcgttgtac aacatgttga tcaactaaca ttacaacatg catgtgaaca
     2341 attaaaaaca tattttaagt aggtgaatga aatggtaaat gaacaaatca ttgatatttc
     2401 aggtccgtta aagggcgaaa tagaagtgcc gggcgataaa tcaatgacac accgtgcaat
     2461 catgttggcg tcgctagctg aaggtgtatc tactatatat aagccactac ttggtgaaga
     2521 ttgtcgtcgt acaatggata tattccgatt attaggcgta gatattaaag aagatgaaga
     2581 taaattggtt gtgaattcac ctggatataa agcttttaaa acacctcatc aagttttata
     2641 tactggaaac tctggcacaa cgactcgatt gttagctggt ttgttaagtg gtttaggtat
     2701 tgaaagtgtt ttgtctggcg atgtttccat aggtaaaagg ccgatggatc gtgttttaag
     2761 accgttaaaa agtatgaatg caaatattga aggaattgaa gacaattaca ccccattaat
     2821 cattaaacca tctgtaatta aaggtattaa ttacaagatg gaagtggcaa gtgcacaagt
     2881 gaaaagtgcc atcttattcg caagtctatt ttctaaggaa gcgaccatca ttaaagaatt
     2941 agatgtaagt cgaaatcata ctgagacgat gttcagacat tttaatattc caattgaagc
     3001 agaagggtta tcaattacta caatccctga agcaattcga tacattaaac ctgcagattt
     3061 tcatgttcct ggcgatattt catctgcagc gttctttatt gttgcagcac ttatcacacc
     3121 aggaagtgat gtaacaattc ataatgttgg aatcaatcca acacgttcag gtattattga
     3181 tattgttgaa aaaatgggcg gtaatattca acttttcaat caaacaactg gtgctgaacc
     3241 tactgcttct attcgtattc aatacacacc aatgcttcaa ccaataaaaa tcgaaggaga
     3301 attagtgcca aaagcaattg atgaactgcc tgtaatagca ttactttgta cacaagcagt
     3361 tggcacgagt acaattaaag atgccgagga attaaaagta aaagaaacaa atagaattga
     3421 tacaacggct gatatgttaa acttgttagg gtttgaatta caaccaacta atgatggatt
     3481 gattattcat ccgtcagaat ttaaaacaaa tgcaacagtg gatagtttaa cggatcatcg
     3541 aataggaatg atgcttgcag ttgcttctct actttcaagc gaacctgtca aaatcaaaca
     3601 atttgatgct gtaaatgtat catttccagg atttttacca aaactaaagc ttttagaaaa
     3661 tgagggataa tataaaatgg aagatatcta taaattaata gacgatatca atctacaaaa
     3721 actagaaaat ttagactctc gtgttaatga agcaataact actgacaacg atgacgcatt
     3781 atttattcta ggagagacac tttacaattt tggattaatg ccacaaggtt tagaagtatt
     3841 ccgcgtgtta tatcacaaat atccagacga aagtgaattg ctgatttatt ttattgaagg
     3901 tttaatgtct gaaaatcaaa ctgacgaagc gttagaatat ttaagttatg ttgaaccatc
     3961 acctgaaaag ttgatgttag aagcagattt atatcaacaa attaatatga tggaagttgc
     4021 tattgataaa ttacaagaag cacttgaact agagccaaat gatccaataa tccattttgc
     4081 gttggctgaa atgttatatt atgatggtca atatttacgt gctacctctg aatacgaaac
     4141 tgttttagaa actggtgaat atcaagttaa tggtgtaaac ttattctctc gtatggcaga
     4201 ttgtagctta caaagtggta actatagtga tgcgattcgc ttatacgatg aaattaatga
     4261 agatgaaatg acttcagaag attatctcaa aaaagccatt tcttacgata aaaatgacat
     4321 cactcaagaa gcaattaaaa taatgactac attactttct aaagatcctg attatattca
     4381 aggctacttg tatttacaat cattatatga aaatgaaaaa aattatccag atgccattga
     4441 aacgggtaaa gagggattac gactaagtca attttataaa gagttaatgt atacaactgg
     4501 atgtttggaa atagaacacg gcgatgctaa tgaaggtgta ttattgttaa aacaagcatt
     4561 agaggttgat aatgcttacc aagaaccatt attgatttta agcgatttat atcgtaatga
     4621 agaagattat gaagcaatca ttgaattatt aacatatgta gatgaagaag atttggatcc
     4681 tacattcatc tggcatttag cgttcgccta tggtcaagaa gagcgagata aagaagctca
     4741 acatttcttt gaattagcat atccaactat gaaaacaaat attgatttta tgagtgatta
     4801 ttatttctat ttaattgaaa ttggtcaaat ggaacaagca attactattt taaatcaatt
     4861 gttggaacta gaaccaagta acgaaaattg gcatgacgaa ttatcaagat tacaatctta
     4921 aaatggggta tttgatgtca gagacactca atcaaattaa agaaagtttt attgaatatt
     4981 tactttttca atatcgattt aagtcgcgta ttgcagtttg ggtgcttaac tatataaaag
     5041 tgaatgaggc gaaattggca aacattcatt ttgtagatac aaaaattaat catcatgaaa
     5101 cattagaaat tgcagaagtg ggaagtcacg cttctgcaat tcaatttact aagcataata
     5161 taaagttaat gaatacaaat gaaatatttg attatatcgc taatcataac ggtgcttttg
     5221 atattcaaat tcacttcaca aacgtgtcaa agcgtgagca aaggctagat gatttaatag
     5281 ttgctcaact aacggaatcg cccagctatc aaacttatct tcatgattta aatagtatgg
     5341 ctatagatcg gcataaacat gcgttattaa tagattactt gctacataat attgatttaa
     5401 gtttacagat gaatgaaaag caacgttttt atcaactaac gcaaatttta aatacactca
     5461 agctagtaaa taaacataat caatttgagg acttagcaga tgacgattaa taaactttgg
     5521 caatatactc tttatcaacg ttcttggcta atgatgctac tcatttgtaa tatattaggg
     5581 atgatttatg gttatatatg gtatggtgaa caattaagtc atacgccgtg gcagtttaag
     5641 atttttgttc cagatagccc aactgcaata ttattcttag ttatttcaat ctcattgata
     5701 ttaatacaaa aacaaaatag tattatcgat gcactggcat ttgtaacatt attcaaatat
     5761 gggatttggg cagttattat gaatatatta tttattattg agcaaggtga tattacagta
     5821 aatggcctgg tattaatgtt ttcccatagt ataatggctg ttcaggcaat ttacttctat
     5881 cctaggttta aacgttctat gattggaatt agtgtagcga tgacttgggt atttttgaat
     5941 gattatattg actattttca ccttcaattt ccatattatg attttataac tacacatgtg
     6001 tggcaaattg gtgtattatc atgttgtctg agtgtttttg gacttttgct ttatattgaa
     6061 ttaaacaaat tattaaagtg taaatgattt gataaaaact gcaaatgaca gtaaaatatt
     6121 tataaaggag agtgagttta ttgtcttcaa tttttactat gataatttat ttcgtaatat
     6181 taatggtgct tcctttatgg gcacaacaca aagttaaatc taactatgag aaatattcac
     6241 aagttagatc aactagtggt aaaacgggtc gtgaagttgc attagagatt ttacatgcaa
     6301 acggtattta tgatgtcgaa gttgttaaag gtgaaggatt cttaacagat cattacgatc
     6361 ctaaaaagaa agttgtttca ctatctcctg caaactatga cagaccatca gttgctggaa
     6421 ctgcaattgc agcccacgaa gttggtcatg ccattcaaga tcaccaagga tattggttct
     6481 taagattcag agcagcatta gtacctgttg caaacctagg gagttcatta agttatatga
     6541 tcatcatgtt aggtattatc ttaacagcga taggtagtgc atttggttca actgctttat
     6601 ggattggtgc tggtttaatg tcattagcgg tattattctc aattgttacg ttaccagttg
     6661 agtttgatgc gagttcaaga gcgatgaaac aaattacggc attgaacatt gtaaatgaaa
     6721 aggaatacaa acatgcgcgt aaagtattat ctgcagcagc aatgacttat gtagcagcaa
     6781 ctgctgtcgc tgtagcagag cttgtacgta ttattttgat tgcgcgttca agtgattaat
     6841 cttatagatt aacaattaac ttaaattaga cacttattaa ctagctaatc acattttgtg
     6901 tggttgctag tttttttgtg cttatttttc taatggtaga ttattcaata tattatttta
     6961 ttgtagtggt acgaatcata aagttataat ctcaatttca attgatttaa tttagttagg
     7021 aatgcattac atttatataa actgaattat taaactacta aaaaaaacaa cttgtcatct
     7081 agtttagcta gagtcagtag attgcactaa acatgcatga gacttcaaat tgatatatac
     7141 tatattagaa atgcaatata aggagaacgt agaaatgaaa tcaatggtag aaatgcaacg
     7201 tgaagttgat gaatatattg gacaatttaa aacaggatat ttttcaccat tagctaactt
     7261 agctagattg actgaagaag tgggcgaact tgcacgtgaa ataaatcata cctatggtga
     7321 aaaaaagaag aaagattcag aggaagcaaa tacgattaaa gcagaattag gtgataattt
     7381 atttgtgttg ttatgtttag cgaattcaat gggaatagat atgacagaga gctttaatga
     7441 aacaatggaa aagtttaata caagagataa aaatcgattc aaaagaaagt gaatataaaa
     7501 atatatgctt tatttggatt tgattaagat taaaaacata gactaatgaa ttgcatcgca
     7561 tgaaatcata ttatttttaa agtaagtata actagataaa tgattatata tacaacacct
     7621 gttttttaga tatttgaaat tataaactca acattactat aaacttaaat ttaaacaatt
     7681 atctacgcat cgagaataat agaaagagaa atagaaagaa aggatgggca agcgaatgaa
     7741 gataggtata acatgttacc cgtccatggg tggctctgga attattgcca cagaattagg
     7801 aattaaatta gcagagcgag gtcacgaagt acattttatc acttcaaata tcccgtttag
     7861 aataagaaaa ccattaccga atatgatttt tcatcaagtt gaagtgaatc aatatgcagt
     7921 ttttcagtat ccaccgtatg atattacgtt aagtactaaa atcgctgaag ttattaaaga
     7981 atatgatctt gatttgttgc atatgcacta tgctgtacct catgcaattt gtgggatttt
     8041 agctcgtgag atgtcaggta aagatataaa aattatgaca acgctacacg gtactgatat
     8101 tactgttttg ggctatgatc attcactcca aggtgcaatt aaatttggca ttgaaaaaag
     8161 tgatattgtg acaagtgtga gtaaatcttt agcgcaagaa acacatgaaa ttatcgaaac
     8221 taacaaagaa attataccga tttataattt tgtacgtgaa aatgagtttc caactaaaca
     8281 taacacagca ttaaaatcac aatttggtat agcaccagat gaaaaagtgt taatacatgt
     8341 atcgaatttt agacaagtaa aacgcataga tactattata gaaacatttg caaaagtacg
     8401 tgaaaagata cctagtaaat taattttact tggtgatggt cctgaattag tgccaatgcg
     8461 tcaaaagacg aaagagttaa acgtagaaga agatgtttta tttttaggga aacaagattg
     8521 cgtaagtgaa ttttaccaat tatctgactt agtattatta ttaagtgaaa aagaaagctt
     8581 tggactaact ttacttgagg ctatgaaaac aggcgtcgtt ccaattggtt caaatgccgg
     8641 tggaattaaa gaggttatta aacatggtga aactggattt gtcgtagatg tgggtgatag
     8701 tgattcagcg agtgactatg ctatccgatt acttgaagac aaggtgttat acaacaaact
     8761 tcagaaaaac atgctagcag atattgctga acgttttgga tcagaactca ttacagatca
     8821 atatgagtat tactatcaaa aaatgctaaa tgagcataac aaaagtaaag gcgagtaata
     8881 atatggataa atcattattt gaacaggcaa ggcctatatt agaacaaatt caagacaatg
     8941 gttttgaagc atattatgta ggtggctctg taagagatta tgtcatggga agaaatattc
     9001 atgatataga tatcacaaca agtgcaacgc cagatgaaat agaatctatc tttagtcata
     9061 cgatacctgt aggtaaagaa catggcacca taaatgtagt ttttaatgat gaaaattatg
     9121 aggtgacaac attccgggct gaagaagatt atgtcgatca ccgtagacca agtggtgtta
     9181 catttgttcg tgatttatac gaagatttgc aacgaagaga tttcacgatg aatgcgatag
     9241 caatggatac agcaaacaaa ttgtatgatt attttgatgg tcaacaagat attaataatc
     9301 gaataataag aactgtaggt atagctgagg aacgattcca agaagatgct ttacgtatga
     9361 ttcgatgttt aagattccag tcacaattat catttgatat tgcaacggaa acattcgaag
     9421 cgatgcgtac acaaatggca gatattaaat ttttatcaat tgagcgtata gtgattgaac
     9481 taactaaatt aatgcgaggt attaatgttg aaaagagttt taatcattta aaatcgctga
     9541 aagcatttaa ttatatgcca tatttcgaac atcttgatat gaatcaaatt aatgtgactg
     9601 aaccaattga tttagaattg ttgattgcta tagtatcagt taaatttgat attaattact
     9661 cattgaagcc tttaaagcta agtaaccgac aagtaaaaga tatcaatcaa tatattcaaa
     9721 ttatgaatgc attaccaagt attattacaa aagaacaatt aaaaatgttt gtttatgatt
     9781 atgatacgaa tctcattaaa aatgtaatgg ttgcagcaga cgtgttaaag gcaaatgata
     9841 ttcaaggaca tgaaccatta atcgttaacc ttcaaacgat tgatgaaaca ttacatcgtt
     9901 tacctatgca taatagaaaa gacatgatgg ttaatggcgg tgtacttatg gcacatttaa
     9961 atgctaaaag tggtccgtgg ttaaaagatc tgctaagaca aattgagata gcgattgtaa
    10021 ctggtaaagt aagcaacgaa gaaactgaaa ttttgaaatg ggtggataat catgtcaaaa
    10081 tatagtcaag atgtacttca attactctat aaaaataaac cgaattatat atctggacaa
    10141 agcattgcgg aatcacttaa tatatcgcgc actgcagtaa aaaaagtgat tgaccaatta
    10201 aagttagagg gatgtaaaat agattcagta aatcataaag gacatttatt acaacaactc
    10261 ccagatattt ggtatcaagg tataatagac caatacacaa aaagttctgc tttgtttgat
    10321 tttagtgaag tatacgattc aatagattct acacaacttg ctgcgaaaaa gtcacttgtt
    10381 ggaaatcaat cttcattttt tatcttgagt gatgaacaaa cgaaaggtcg cgggcgattt
    10441 aatagacatt ggagttcttc aaaagggcaa ggactatgga tgtctgttgt attaagacct
    10501 aacgttgcat tctcaatgat atctaaattt aatttattta ttgcattagg gataagagat
    10561 gcgattcaac attttagtca agatgaagtc aaagtgaaat ggccgaatga tatatatatt
    10621 gataatggta aagtgtgtgg tttcttaact gaaatggttg ctaataatga tggtatagaa
    10681 gcaataatat gtggtatagg tattaatttg acgcaacaac tagaagactt tgatgaaagt
    10741 attagatata gagcaacaag tatacaatta catgataaaa ataaattaga tagatatcaa
    10801 tttttagaga gattacttca agaaattgaa aaaagatata atcaattttt aacgttacct
    10861 ttttctgaaa ttcgtgaaga atatattgca gcttctaata tctggaatag aacgttgcta
    10921 tttacagaaa atgataaaca gtttaaagga caagcaattg atttagatta cgatggctat
    10981 ctaattgtta gagatgaagc gggtgaatca caccgtttaa ttagtgcaga tatagatttt
    11041 taacactaaa gcaaggagag atagctatgg gtatggcaac ctatgccgtt gtggatttgg
    11101 aaacaacagg caaccaatta gattttgacg atatcattca aattggtata acgtttgtgc
    11161 gcaataacca aattattgat acatatcatt ctatgattag aacaaattta gagattccgc
    11221 ctttcattca agcgttaaca tccattgaag aaaatatgct acaacaagcg ccatatttca
    11281 accaagttgc agaagaaatc tacgataaga taaaagattg catttttgtc gctcataatg
    11341 tagattttga tttaaatttt attaaaaagg catttaaaga ttgtaatatt caatatcgtc
    11401 ccaaaaaagt tatagataca ctagaaatct ttaaaatagc ttttcctaca gataaaagtt
    11461 atcaattaag tgaattagcg gaggcacatg gtattacctt agcaaatgcc catcgagctg
    11521 acgaagatgc cgctactact gctaaattga tgatattagc ttttgaaaaa tttgaaaagt
    11581 taccgttaga tactttaaag caattatatt atttaagtaa acagttgaaa tacgatttgt
    11641 atgatatttt ctttgagatg gttaggcaat atgatgcaaa gccattggat aaatcgtacg
    11701 aaaagttcga acagataata tatcgtaagc aagttgattt taaaaagccg acaacaaatt
    11761 ataatggcag cttgaagtct ttatatagca aagctgtaga tcaacttggc ttaacatatc
    11821 gaccgcaaca attatattta gcggaaacta tattagatca gctcatgcat agtgaaaaag
    11881 caatgattga agcatcacta ggcagtggta aatcattagc atatttactt gcggccttga
    11941 tgtataatat tgaaactggg aagcatgtca tgatttctac caatactaaa ttactgcaaa
    12001 gtcaattgtt agaaaaagat attccagcaa tgaatgaagc gttgaatttt aaaattaatg
    12061 cactattgat taaaagtaaa agtgattaca tttcattggg acttattagt caaattttaa
    12121 aagatgacac aagtaattac gaagtgaata tcttgaaaat gcaattgctg atttggatta
    12181 cagagacgcc gtccggagat atacaggaat taaatttaaa aggtggtcaa aagatgtatt
    12241 ttgaccagaa aattgaaaca tatgtaccgg cacgtcacga tgttcattat tataatttta
    12301 ttaagcgaaa tgcacagaac attcaaattg gtattacaaa tcatgcacat ttaattcatt
    12361 ctgatgttga aaattcaata tatcaattgt ttgatgattg tatagtggat gaagcacatc
    12421 gcttaccaga ctatgcctta aatcaagtga caaatgagtt gagttatgct gatattaaat
    12481 atcagcttgg tttgattggt aaaaacgaaa atgaaaaatt gttaaaggca attgatcaac
    12541 tggaaaagca gcggattttg gaaaaattag atattgcacc aattgatatt tttggcttga
    12601 aggctagtat gaatgagatt catgagttaa atgagcaatt gttctcaacg atatttacga
    12661 ttatcaatga ttctgatgtt tatgatgatg atattcatcg cttccataat gtatttactt
    12721 ttgaaacaaa agacattttg aaggatttac atgcgattat cgataagtta aataagacgc
    12781 tagaaatatt caatggcatt agtcataaaa cagttaaatc attacggaaa cagttgttgt
    12841 atttaaaaga taaatttaaa aatatcgaac aaagtttaaa agctggacat actagtttca
    12901 tttcaattaa aaatctatct caaaaatcaa caatacgttt atatgtgaaa gattacgctg
    12961 taaaagatgt attaacgaaa caagtattgg aaaaatttaa atcacttatt tttatatctg
    13021 gtactttaaa atttaatcat tcgtttgatg ctttcaaaca gttattcaac aaagatgttc
    13081 actttaatac atttgaagtt aacacatcgt tacaaagtgc aaaaaataca agtgtattta
    13141 taccgagtga tgtagcatct tatcaatata aaaatataga tgagtatgta gcatcgattg
    13201 taagctatat tattgaatat acgactataa cgtcatcaaa atgtttagtc ttatttacga
    13261 gttataaaat gatgcatatg gtacaagata tgctaaatga attgccagaa tttgaagatt
    13321 atgttgtatt aacacaacag cagaaccaaa attataaaat agtacaacag tttaataatt
    13381 ttgataaagc catactgctt ggtacgtcaa cattttttga aggctttgat tttcaagcaa
    13441 atggtattaa atgtgtgatg atagcgaagt taccgtttat gaacaagcat aatgcaaaat
    13501 attggcttat ggattctgaa ttcacttcaa ctttcaagga atatgtatta cctgatgcag
    13561 ttacacgttt tagacaagga cttggaagat taattagaaa tgaaaatgat cgcggcataa
    13621 tagtttcatt tgatgatcga ctcataaaca gtaattataa aaactttttt gaacaaacac
    13681 ttgaaaatta tcgtcagaaa aaaggcgaca ttcaacagtt tggaaaatta ttaagacaaa
    13741 ttcaaaaaaa gaaaaagtaa aatacaaagt aaaattcttg tgacatgaca tgtgctatgt
    13801 tctgttacaa gaatttttgt ttggtcgtca aaatttgagt tttataatta aacttgaatg
    13861 ttagcacaaa aaacgacctt catactaata atattgaggc tttattatat attttgtctg
    13921 aaactgatga ttgtattgtc ttaatataaa ttaatgttca aatctgtgct taaatcatta
    13981 ccatttacac gttatctttt agtctagttc tagaacttga aagcggattt tgttaaaatg
    14041 aatgagtatt gtataacgaa aaaataaata ggagaatggt tatgaaaaca acgattaaac
    14101 aagcgaaaga tcatttaaac caagacgtta caattggtgc ttggctaaca aataaacgtt
    14161 caagtggtaa aatcgccttt ttacaattac gtgatggaac aggctttatg caaggcgtag
    14221 tagttaaatc agaagttgat gaagaggtat tcaaacttgc gaaagaaatt actcaagaat
    14281 catctctata tgttacaggc acaattacag aagataatcg ttctgactta ggatacgaaa
    14341 tgcaagtgaa atcaattgaa gttatttcag aagcgcatga ctatccgatt acacctaaaa
    14401 atcatggtac agaattctta atggatcacc gtcatttatg gttacgttct aaaaaacaac
    14461 atgctgtaat gaaaattaga aatgaagtta ttcgtgcaac gtatgaattt ttcaacaaag
    14521 atggatttac aaaggttgat ccaccaattt tgacagcaag tgcaccagaa ggtacaagtg
    14581 aattattcca tactaaatac tttgatcaag atgcgttttt atctcaaagt ggtcagttat
    14641 acttagaagc tgcggcaatg gcacacggaa aagtattttc atttggtcca actttcagag
    14701 ctgaaaaatc aaaaacacgt agacacttga tcgagttctg gatgattgaa ggggaaatgg
    14761 ctttcacaaa tcatgctgaa agtttagaaa ttcaagaaca atatgtaaca catgtagtaa
    14821 aatcagtttt agaaaattgt aaactagagt tgaaaatgtt agagcgtgat acatcaaaac
    14881 ttgaaaaagt tgcgacacca ttccctagaa tttcatatga tgatgcaatt gagttcttga
    14941 aagcagaagg ctttgatgat attgaatggg gcgaagattt tggtgcgcca catgaaacag
    15001 ccattgctaa tcattatgat ttaccggtat ttattactaa ttatccaact aaaattaagc
    15061 ctttctatat gcaaccaaat cctgagaatg aagaaactgt attatgtgca gacttaattg
    15121 cacctgaagg atacggtgaa attataggtg gttctgaacg tgtggatgac ttagaattgt
    15181 tagaacaacg cgttaaagaa catggattag acgaagaagc atatagttac tacttagact
    15241 tacgtcgtta tggtagtgta ccacactgtg gatttggttt aggtttagag cgtacagtcg
    15301 catggatttc tggtgttgaa cacgttcgtg aaacagcgcc attcccaaga ttattaaacc
    15361 gtttatatcc ataagtttta gggtcgttac ttaaaattta tattatgata ataaaaagaa
    15421 tcgtccagcc tatattaata gctgggcgat ttatgcttgg aaaatgaaga atgataacgg
    15481 cttatattaa tcatgaacaa acttttgttt agtaactata tttggaaaat aaatagctcg
    15541 tctgattata cataaagttc attgttaaaa tctagtttta aaaaaatact gttatttaaa
    15601 atttgtttgc tactagttaa atatattaag cattttagtc gaaaattaaa gaaaatgatt
    15661 tatactatta agtaatggtt aaatttaaaa gggggcacga acatggataa atatcaatta
    15721 aaagcaagac ctgtagtgat acgaagagaa ttattagacc attacagtga cttaggttta
    15781 gatgagcaag atttagtcat tttgcttaaa ttgatttatg cttccgaaac atcaaacaaa
    15841 cagccttcaa ttgaattact tcagaagggc tcaactatgc aaccacgtga tattacaatg
    15901 gttattcaaa atttgattca acgtgaattg ctagaattac aagttcaaaa agatgaagaa
    15961 ggtagattta ctgaatatat gaatctagat ccttttttcg aaaaattaag tcatatattg
    16021 aagcaacaaa gtatggaaac gaaagagcaa aatagtaaag aaaaatttaa acaattattt
    16081 agagttttag aggatacatt tgcaaggccg ttatctccat atgaaataga gacgttaaat
    16141 caatggattg atgttgataa acacgatact gcaattatac aagcggcatt agacgaggcg
    16201 aatagtttga ataaacttag ctttaaatat atggatcgta ttttacttaa ttggaagaaa
    16261 aacaatgtca aaacaattga cgattctaga aaaataagag aaaaatttaa taaaccgaaa
    16321 atgacgcaca cagttaaaac ggtacctaaa tttgattggt taaacgggga gaaccttgat
    16381 ggtaagtaag aaaaaagcat tagaaatgat tgatgttata gcaaatatgt ttccggatgc
    16441 agaatgtgaa ctaaaacatg ataatccgtt cgaattaact attgctgtat tattgtcagc
    16501 gcaatgtaca gacgttctag taaatagagt aacaactgag ctgtttaaaa aatacaaaac
    16561 gcctgaagat tatttggcag tcagtgatga agaacttatg aatgatattc gttctatcgg
    16621 tctgtatcgt aacaaagcta agaatattaa gaaactttgt caatctttga ttgatcaatt
    16681 taatggagaa ataccacaaa cacataagga attagaaagt ttagcaggtg taggacgtaa
    16741 aactgctaat gtagtcatga gtgtagcatt tgatgaacct tcattagctg ttgatacgca
    16801 tgtagaacgt gtttctaaac gcttaggtat taatcgttgg aaagataatg tgagacaggt
    16861 agaagaccgt ttatgttctg tcatacctag agatagatgg aataggagcc atcatcaact
    16921 cattttcttt ggaagatacc actgtttagc ccgaaaacct aaatgcgata tttgtccatt
    16981 attagaagat tgtagagaag gacaaaaacg ttataaagct agtttgaaag aagcgtgata
    17041 atatgacaaa tattacaaaa gaggtatttg ataatttaga acaaaagatt gatgtatttg
    17101 ccaaaaataa gacattaggt tctagtgaag caaaacctta cttggatgaa taccatagta
    17161 aaattattga ttattttaag caggttaatg acataactgg caatattgat tttgataatt
    17221 taaatcaata tcctgttgtg ccaatgaatt ttaaagaaag atatgattat atgattgaac
    17281 gtaaatatca ttttatgggc tatcgacaaa tgaagacctt taaaactgaa ttgattaaaa
    17341 tgaatgctag ttatcaaaca aggttaaaga ataagcaggt atagacaagt ttgatcattt
    17401 cctaatgttg aaaagatgta aaactaattc atgcacacat atttgtattt atgttaggtg
    17461 tcatgtcatt acaacgtctt aatataaaaa atgtccatct acatcataat ggatatcttg
    17521 aggtatggtt taagatatct atttcgaatc gtaggtggat ttcttatatc tatgattgtg
    17581 aatatacgtt gcaaacatgt tgatatttca ctgattaacg tactcaggga attgataaac
    17641 atatcaattt aaaacaacgt ctaagatgac tatgagaaaa tataatcata attttaaaaa
    17701 tgggtacaac cacttttgca atttcaataa ttttacatag ctaaagatgt gaatatgaaa
    17761 gactatttag atgtttcaaa atgtatgtga ataactgatt ttacggtttc tactaatata
    17821 gcaatagcgt atgaatacat ataaaaaaac ctcagctaca ccataaatat ggtataactg
    17881 agttttttat gttgagtgga ttagttgaat atacctgtta atccaccgct gtttcaagat
    17941 tgttgagatc tagtattgtt atttgattgt gcagtaccac tgctgtttgc tgatgtgtca
    18001 ctaccaccgt gtgtactacg atttgtagtg ttgttatctt gactaccaga aacattgatt
    18061 gatgggatac taccacttac tgagctagga cgtttaaagt cttcgccatc tctagatgaa
    18121 atttttgaca tcacattttc atataagaac tgtggatatt cttgttggct atgtcccaca
    18181 aatgagtttt caccatattg tttaacttta ctgaagccca tccacactga catagtgtat
    18241 tgaggtgtaa agccgttaat ccacacgtct ttcgctgcat tatcaggtaa attatattgt
    18301 gaataagttt cagcaccgta agtaccagta cctgtcttag cacccatatt tactccagat
    18361 acaccatggc catatgcaga accatatggt ttaaatgtac cttttagcat ctcagctaac
    18421 atgtatgcag tgtaatcact catcgcttta tggctagtat gatcgtattc gattgtttca
    18481 ccatcacgag taactacttt ttgaattgaa tgcgcgttgt tataagtacc accgttagcg
    18541 attgcagcaa atgctgatgc taattgtgtt ggtgagaatt ctgaagcaga accaccaagt
    18601 acttcagatg gaccaatatc gccttcgtag tttaagccaa gtttggcagc gaatttctta
    18661 ggtgcatcat taccagcatt ttgcttaact gattgccaag cttttaaagc tgggatattg
    18721 aaactttgtc gtaaagcatc ataaatagat acagtaccgt gactcttcgt atcatagttt
    18781 ctaaatgtag atccatcaac ttgatatgaa gattcatctt gaatcgcatg gtttgttgcc
    18841 catttcatat tttcaatggc aggtccatac gctaagaaag gttttaaaga tgaaccagta
    18901 gggtgtggat ctgttgcttg gtttctgtta acgacgtctt tgaaatcacg tccgccagat
    18961 atagcaacta aaccaccagt tttactatca agaatcgttg caccaacttg ttggtcttta
    19021 ttcttgtaga agctaccatt atcaacatca ttttgtaatg ttttttgaac atctttatcc
    19081 atgtttgtat aaattttaat accactttgt aatacattac ctaaattttc atctttgaat
    19141 gctttattat tcattaattc agattttaca aagttaacgt atgaattata ctcagaatct
    19201 tgatttgtat caatgttttg acgttcttca gcagtacgat ttactaagtt cgcttttaaa
    19261 tcgattttct tagcatcttc ccactgttta tctgtaatgc gtttatgata atgcattaag
    19321 tataaaacag tgtttttacg atcttcagca gcttttggat gatcataaat attatagttg
    19381 tttggaacct gaggtaaacc ggctaaataa gcttcttccg ctaagtttaa atcttttaaa
    19441 tctttattaa agtaatactt agcagcagct ttaatacctg ttacgccatc agaatagtaa
    19501 attttattta gatatacttg gaagatatca tctttactat actcttgttc taaacgatat
    19561 gataagtatg cttcttgagc tttacgtcca atagatttat gttgtgataa aaatgcatct
    19621 ttaacaactt gttgtgttaa tgttgaggca ccttcagaac caaatccacc agtcaagttc
    19681 ttaccaattg caccgaataa acgtttataa tcaagtgcgc catgttcgta gaaacgattg
    19741 tcttcagttg caagtactgc gtctttcatt gatttcggca cgtcttttaa atttacatgc
    19801 tcatgtcttt ggccattatc taatgtttta acaagttctc cgttcttgtc atatatcttt
    19861 gcaggaatcg gatcttgtaa tttagcttcg gtaaaagcag gtgctttcca agcataataa
    19921 gcaaacaata agatacctag taaaagaaca acaaaaaatg caataatcat gaagccaata
    19981 atcttaataa tcgttctctt cacatttcta ttcttttttg agttggattt cccaccatta
    20041 ttaccatttt tcttaggctg agaagatcct ttgttttccg tcatacgcgg tcctcacttt
    20101 catctaatat caacttatca actgctttta gatagtctaa tcttggttga tactgataag
    20161 gaatatggta accatttttt cgtatttcat caactgttat agacttttta atattatctt
    20221 tatatctctt ccaaaatatt tcgaattttg aatagggtaa aagataaact tcatctagcg
    20281 ttttaaaacg aatcattaaa aacacaatac ctttttgttg atatgcattt ttcatatgtt
    20341 cgacttgatg gtcatgaata ttatttaaag gaaaggacgt cttgttttta gtttcctttg
    20401 cttcaaaatc aatataataa ccttgataaa cgccgttgta atcagttgtt gaaggtgtac
    20461 gaaaataagc ttcgttaatc acagctttac tccgcttagg atagttgaca ttaactattt
    20521 gtactggcgt aggctttttg tgaataactg caatgtcgct tttaagataa aacgcatttg
    20581 aatgttcgat atctttttca agtgacatac cacgtccacc atactcaata ttactaaagg
    20641 cagcggtctt tttccctccg tctatagcac tatttttacg atatggttta ccatttggat
    20701 aattcataaa tttcaccaca caagtttggt agtaaacgtt gtttattata cacaacatta
    20761 tagtgaaata ccaaacttaa attagtataa atgcgaaaga cacaatacac caaagcttat
    20821 tttaacgtga tttcagcatt aacacaataa tattataata catttaccgt taatgttgga
    20881 attttacaca caattttaat taattgaaaa gttttgctta taaaaatgaa tactcattac
    20941 aatatttttg aattttgtta taaa
//
LOCUS       DAKSQE010000040        20619 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.040, whole genome shotgun sequence.
ACCESSION   DAKSQE010000040 DAKSQE010000000
VERSION     DAKSQE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20619
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.040"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            656..1951
                     /gene="purB"
                     /locus_tag="O0V33_001255"
     CDS             656..1951
                     /gene="purB"
                     /locus_tag="O0V33_001255"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCX8975896.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
     gene            2060..2362
                     /locus_tag="O0V33_001256"
     CDS             2060..2362
                     /locus_tag="O0V33_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975897.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            2545..3237
                     /locus_tag="O0V33_001257"
     CDS             2545..3237
                     /locus_tag="O0V33_001257"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HCX8975898.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVAFHNGTLLESLK
                     TYGHSIDFEEVIFEGYVVCNADSKVAKHTKANTDLTTEDLEAYAQMVNHLYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            3234..5426
                     /gene="pcrA"
                     /locus_tag="O0V33_001258"
     CDS             3234..5426
                     /gene="pcrA"
                     /locus_tag="O0V33_001258"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCX8975899.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVSSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            5430..7433
                     /gene="ligA"
                     /locus_tag="O0V33_001259"
     CDS             5430..7433
                     /gene="ligA"
                     /locus_tag="O0V33_001259"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCX8975900.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEFKTVDSPTVRVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVSDIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            7446..8645
                     /locus_tag="O0V33_001260"
     CDS             7446..8645
                     /locus_tag="O0V33_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HCX8975901.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGPTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(8734..10272)
                     /gene="putP"
                     /locus_tag="O0V33_001261"
     CDS             complement(8734..10272)
                     /gene="putP"
                     /locus_tag="O0V33_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCX8975902.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIVRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLVRGEEKAKTHQKEFVMVGRLSVLAV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
     gene            10639..10941
                     /gene="gatC"
                     /locus_tag="O0V33_001262"
     CDS             10639..10941
                     /gene="gatC"
                     /locus_tag="O0V33_001262"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCX8975903.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            10943..12400
                     /gene="gatA"
                     /locus_tag="O0V33_001263"
     CDS             10943..12400
                     /gene="gatA"
                     /locus_tag="O0V33_001263"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCX8975904.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADANDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            12413..13840
                     /gene="gatB"
                     /locus_tag="O0V33_001264"
     CDS             12413..13840
                     /gene="gatB"
                     /locus_tag="O0V33_001264"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCX8975905.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLSGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
     gene            13879..14025
                     /locus_tag="O0V33_001265"
     CDS             13879..14025
                     /locus_tag="O0V33_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975906.1"
                     /translation="MVADLDAIEGLFISIEVILLLTLFIVHVNGKNINFINALALIIL
                     RQTV"
     gene            14663..15610
                     /locus_tag="O0V33_001266"
     CDS             14663..15610
                     /locus_tag="O0V33_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCX8975907.1"
                     /translation="MRKRARIIYNPTSGKEQFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
     gene            15691..17052
                     /gene="rlmD"
                     /locus_tag="O0V33_001267"
     CDS             15691..17052
                     /gene="rlmD"
                     /locus_tag="O0V33_001267"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCX8975908.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDMG
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMVIFVTN
                     GKNWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPHAKHVYG
                     VEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPSTQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            17222..17752
                     /locus_tag="O0V33_001268"
     CDS             17222..17752
                     /locus_tag="O0V33_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCX8975909.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSAGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPTQ"
     gene            17999..19069
                     /gene="dinB"
                     /locus_tag="O0V33_001269"
     CDS             17999..19069
                     /gene="dinB"
                     /locus_tag="O0V33_001269"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCX8975910.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGMTVIDYRNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSTRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
     gene            complement(19136..19690)
                     /locus_tag="O0V33_001270"
     CDS             complement(19136..19690)
                     /locus_tag="O0V33_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HCX8975911.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKVHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
     gene            19907..19999
                     /locus_tag="O0V33_001271"
     CDS             19907..19999
                     /locus_tag="O0V33_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975912.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            complement(20081..20581)
                     /gene="ftnA"
                     /locus_tag="O0V33_001272"
     CDS             complement(20081..20581)
                     /gene="ftnA"
                     /locus_tag="O0V33_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HCX8975913.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEEAMFETHINYLTRIGDDSNALYLYEKELGT
                     RTFDEE"
BASE COUNT         7585 a         2995 c         3814 g         6225 t
ORIGIN      
        1 atgttagtcg attttgaaag aaacgtagtt aattgatgtc tgtctataaa aatttactat
       61 taagaaaatc tctgagtact tttctataat cactttcgta tttatacgac tcgggcagtt
      121 caatattaat tgcttatatt taaaatgcca tacctatgca attctttttg tgagtagaaa
      181 atttgttata ttagaagcga ataattttaa aacacattat ggaatgtaag aaggaatatt
      241 atgaaactag gaaaagtaga ttgttgaaaa ttctatacaa tatgagaagt aaagtgaata
      301 atatctggta ttactaaagc tattgattgt cctaattgta ataaaaaggt ggatagtcta
      361 ggtgagggga gaaaaactaa aatgcaataa tatattctta atggatcatt ataaatacca
      421 attagattaa agtgttgcga acatttacaa ttagaaatca gtattaatct gccatagata
      481 attgtattaa tgtaaaaaat aaaatagaat gagaatatac ggaaattttt tatacattta
      541 taaccaattc tgttaaactg tgaagagtag agttaaaaac gaacttttta tttattttta
      601 ttaaaaatgt tctgtttttg aaaattcgag tatacatacg ataggagtta aaaaaatgat
      661 tgaacgttat tctagagaag aaatgtctaa tatttggact gatcaaaatc gctatgaagc
      721 atggttagaa gtggaaattt tagcatgtga ggcatggagt gaattaggac atataccgaa
      781 agctgacgta caaaaaattc gtcaaaacgc aaaggtaaat gtcgaacgtg cacaagaaat
      841 tgaacaagaa acgcgccatg atgttgtagc ctttactaga caagtttctg aaacgctagg
      901 tgaagaacgt aagtgggtac attatggttt aacttctact gatgttgtag atacagcttt
      961 aagtttcgtt attaaacaag caaatgacat tattgaaaaa gatttagaaa gatttatcga
     1021 cgttttagct gaaaaagcaa aaaattataa atatacattg atgatgggac gtacacacgg
     1081 agtgcatgca gaaccaacta catttggtgt caaaatggca ttgtggtaca cggaaatgca
     1141 acgtaactta caacgtttca aacaagtaag agaagaaatt gaagtaggga aaatgagtgg
     1201 tgcagtaggt acttttgcta acattcctcc tgaaatagaa agttatgtgt gtaaacattt
     1261 aggtattggc acagcgcctg tatcaacgca aacattgcaa cgcgatcgac atgcatacta
     1321 tattgcgaca ttagcattaa tcgcaacttc actagaaaaa tttgccgttg aaattcgtaa
     1381 ccttcaaaaa accgaaacaa gagaagttga agaagcattt gcaaaaggtc aaaaaggttc
     1441 atctgcaatg cctcataaac gaaatccaat tggttctgaa aatatcacag gtatttcaag
     1501 agtaattcgt ggttatatta ctacagctta tgagaatgtg ccattatggc atgaaagaga
     1561 tatttctcat tcttcagctg agcgtattat gttaccagat gttacaatag cactagatta
     1621 tgcattgaat cgtttcacaa atattgtgga ccgtttaact gtatttgaag ataatatgcg
     1681 taataacata gataaaacat ttggtctgat tttctcacaa cgtgtcttat tagcattaat
     1741 taataaaggt atggtccgtg aagaagcata cgacaaagta caaccaaaag cgatgatatc
     1801 ttgggaaacg aaaacacctt tccgtgaatt aatcgaacaa gatgagtcta ttacaagtgt
     1861 tttaacaaaa gaagaattgg atgaatgttt cgatcctaaa catcatttga atcaagttga
     1921 cactattttc gaacgcgctg gattagcata aaattaaata aagtaacgat tcagatttaa
     1981 taaaatataa atcgctacac tttaataggt taatgcgtta aaatgacata aagaataaaa
     2041 ttataggggt tgtttcgata tgcaaattga aaaattacga ggtgcagcgt tagatgaatt
     2101 gtttgatgca atactaacgt tagaaaatag agaagaatgt taccaatttt tcgatgattt
     2161 gtgtactgta aatgaaattc aatcactgtc tcaacgatta caagttgcta aaatgattaa
     2221 gcaaggttat acctatgcaa cgattgaaca agaatctgga gcatcgactg caacgatttc
     2281 tagagtgaag cgttcattac aatggggtaa tgatgcttat acaatgattt tagatcgtat
     2341 gaatattgaa acaaatgaat aataaatgaa cgataaacga acgataaaca atggttatct
     2401 atctgtacta ataaggtaga taatcattgt tttttcacga aaaaatttac agagtaaaag
     2461 aacttaaatt tcatattaag tctttagaac tcgacactta aaaatgctat aatcatatgt
     2521 atgttaaaaa aggagtttcg gaaaatgtat gacattaaaa aatggcgcca tatttttaaa
     2581 ttagacccag ctaagcatat ttcagatgat gatttagatg cgatttgtat gtctcaaaca
     2641 gatgcaatta tgattggtgg aactgatgac gttactgaag ataatgtcat tcatttaatg
     2701 agcagggtaa gaagataccc attgccttta gtacttgaaa tatcaaacat cgaaagtgta
     2761 atgcctggtt ttgattttta ttttgtacct acagtactga acagtacaga tgttgcattt
     2821 cacaatggta cattattaga atcgcttaaa acatatggac atagtataga ttttgaagaa
     2881 gtaatatttg aagggtatgt cgtgtgcaat gctgatagca aagtggcaaa acataccaaa
     2941 gcaaatacag atttaacaac agaagattta gaagcatatg cccaaatggt caatcatctg
     3001 tatcgattac cggttatgta tatagagtat agtggcattt atggcgacgt atcaaaggtt
     3061 caagctgtct cagaacatct aacagaaacg caactttttt atggtggcgg tatttcctca
     3121 gaacaacaag cgacagagat ggcagctatt gcagatacaa ttatcgtcgg tgatattatt
     3181 tataaagata ttaaaaaagc tttaaaaaca gtaaaaataa aggagtctag taaatgaatg
     3241 cgttattaaa tcatatgaat acagagcaaa gtgaagctgt aaagacaaca gaaggaccat
     3301 tgttaattat ggcaggtgct ggttcaggga agacacgtgt tttaacacat agaattgctt
     3361 atttattaga cgaaaaagat gtctcaccat acaatgtttt ggctattact tttacaaata
     3421 aagcagcaag agaaatgaaa gaacgtgttc aaaaattagt aggtgatcaa gcagaagtga
     3481 tttggatgtc aacattccac tcaatgtgtg tgcgtatttt acgtcgtgat gcagatcgaa
     3541 ttggtataga acgcaatttt acgataattg atcctacaga ccaaaaatct gttattaaag
     3601 acgtcttaaa aaatgaaaat attgatagta aaaagtttga accacgtatg tttatcggtg
     3661 cgatcagtaa tttgaaaaat gaacttaaaa cacctgcaga tgctcaaaaa gaagccacag
     3721 attatcactc gcaaatggta gcaacggttt atagtggata tcaacgccaa ttgtcacgta
     3781 atgaagcgtt agattttgat gaccttatta tgacaacgat taacttattt gagcgtgtac
     3841 cagaagttct agaatattat cagaacaaat tccaatatat tcatgtagat gagtatcaag
     3901 ataccaataa agcacaatac acattagtta aattattagc aagtaagttt aaaaacttat
     3961 gtgttgtagg tgactcagat cagtcaattt atggttggcg tggtgctgat attcaaaata
     4021 tcttatcatt tgaaaaagac tacccagaag cgaatacaat ctttttagag caaaattatc
     4081 gttcgacgaa aacgatttta aatgcggcta acgaagtgat taaaaataat tctgaacgta
     4141 agccaaaagg actgtggact gcaaatacga atggtgagaa aattcattac tatgaagcaa
     4201 tgacagaacg tgatgaagcg gaatttgtaa tacgagaaat tatgaagcat caacgtaatg
     4261 gtaagaaata tcaagatatg gcaattttat atagaacaaa tgcacaatca cgtgtacttg
     4321 aggaaacatt catgaaatct aatatgccat acacaatggt tggtggccaa aagttctatg
     4381 accgtaaaga aattaaagat ttattaagtt atttgcgtat aattgccaat agtaatgatg
     4441 acattagttt gcaacgtatt attaatgttc caaaaagagg tgtaggtcct tcatctgttg
     4501 aaaaagttca aaattatgca cttcaaaaca atatcagtat gtttgatgca cttggagaag
     4561 ctgattttat cggattgtca aaaaaggtga cacaagagtg tcttaatttt tacgagttaa
     4621 tccaaagctt gataaaagaa caagaatttt tagaaattca tgaaatagtt gatgaagtat
     4681 tacaaaagtc tggctaccga gaaatgcttg aacgtgaaaa tacactagaa tctcgaagta
     4741 gattagaaaa catcgatgaa tttatgtcag taccaaaaga ctatgaggaa aataccccat
     4801 tagaagaaca gtcattaatt aactttttaa cggatttatc gttagtagct gatattgatg
     4861 aggcagatac tgaaaatggc gtaacactaa tgacgatgca ctcggctaag ggccttgaat
     4921 ttccaatcgt ctttataatg ggaatggaag aatctttatt cccacatatt agagcgatta
     4981 agagtgaaga tgatcatgaa atgcaagaag aacgtcgtat ttgttatgta gcaatcacaa
     5041 gggctgaaga ggtgttatac atcactcatg cgacatcaag aatgttattt ggtcgccctc
     5101 agtcaaatat gccatctaga tttttaaagg aaattccaga atcactatta gaaaatcatt
     5161 caagtggcaa acgacaaacg atacaaccta aggcaaaacc ttttgctaaa cgtggattta
     5221 gtcaacgaac aacgtcaacg aaaaaacaag tatcgtcatc tgattggaat gtaggtgaca
     5281 aagtgatgca taaagcctgg ggagaaggca tggtgagtaa tgtaaacgag aaaaatggct
     5341 caatcgaact agatattatc tttaaatcac aagggccaaa acgtttgtta gcgcaatttg
     5401 caccaattga aaaaaaggag gattaaggga tggctgattt atcgtctcgt gtgaacgagt
     5461 tacatgattt attaaatcaa tacagttatg aatactatgt agaggataat ccatctgtac
     5521 cagatagtga atatgacaaa ttacttcatg aactgattaa aatagaagag gagcatcctg
     5581 agtttaagac tgtagattct ccaacggtta gagttggtgg tgaagcccaa gcctctttca
     5641 ataaagtcaa ccatgacacg ccaatgttaa gtttagggaa tgcatttaat gaggatgatt
     5701 tgagaaaatt cgaccaacgc atacgtgaac aaattggcaa cgttgaatat atgtgcgaat
     5761 taaaaattga tggcttagca gtatcattga aatatgttga tggatacttc gttcaaggtt
     5821 taacacgtgg tgatggaaca acaggtgaag atattaccga aaatttaaaa acaattcatg
     5881 cgataccttt gaaaatgaaa gaaccattaa atgtagaagt tcgtggtgaa gcatatatgc
     5941 cgagacgttc atttttacga ttaaatgaag aaaaagaaaa aaatgatgag cagttatttg
     6001 caaatccaag aaacgcagct gcggggtcat taagacagtt agattctaaa ttaacggcaa
     6061 aacgaaagct aagcgtattt atatatagtg tcaatgattt caccgatttc aatgcgcgtt
     6121 cgcaaagtga agcattagat gagttagata aattaggttt tacaacgaat aaaaatagag
     6181 cgcgcgtcag cgatatcgat ggcgttttag aatatattga aaaatggaca agccaaaggg
     6241 aatcattgcc atacgatatc gatggtattg tgataaaggt taatgattta gatcagcagg
     6301 acgaaatggg attcacacaa aaatcaccaa gatgggccat tgcttataaa ttcccagctg
     6361 aggaagtggt aactaaatta ttagatattg aattaagtat tggacgtaca ggtgtagtca
     6421 cacctactgc tattttagaa ccagtaaaag tagctggtac aactgtatca agagcatcct
     6481 tgcacaatga agatttaatt catgacagag atattcgaat tggtgatagt gttgtagtga
     6541 aaaaagcagg tgacatcata cctgaagttg tacgtagtat tccagaacgt agacctgagg
     6601 atgctgtcac atatcatatg ccaacccatt gtccaagttg tggacatgaa ttagtacgta
     6661 ttgaaggcga agtagcactt cgttgcatta atccaaaatg ccaagcacaa cttgttgaag
     6721 gattgattca ctttgtatca agacaagcca tgaatattga tggtttaggc actaaaatta
     6781 ttcaacagct ttatcaaagc gaattaatta aagatgttgc tgatattttc tatttaacag
     6841 aagaagattt attaccttta gacagaatgg ggcagaaaaa agttgataat ttattagctg
     6901 ccattcaaca agcaaaggac aactctttag aaaatttatt attcggtcta ggtattaggc
     6961 atttaggtgt taaagcgagc caagtgttag cagaaaaata tgaaacgata gatcgattac
     7021 taacagtaac tgaagcggaa ttagtagaaa ttcatgatat aggtgataaa gtagcacaat
     7081 ctgtagttac ttatttagaa aatgaagata ttcgtgcttt aattcaaaaa ttaaaagata
     7141 aacatgttaa tatgatttat aaaggtatca aaacatcaga tattgaagga catcctgaat
     7201 ttagtggtaa aacgatagta ctgactggta agctacatca aatgacacgc aatgaagcat
     7261 ctaaatggct tgcatcacaa ggtgctaaag ttacaagtag cgttactaaa aatacagatg
     7321 tcgttattgc tggtgaagat gcaggttcaa aattaacaaa agcacaaagt ttaggtattg
     7381 aaatttggac agagcaacaa tttgtagata agcaaaatga attaaatagt tagaggggta
     7441 tgtcgatgaa gcgtacatta gtattattga ttacagctat ctttatactc gctgcttgtg
     7501 gtaaccataa ggatgaccag gctggaaaag ataatcaaaa acataacaat agttcaaatc
     7561 aagtaaaaga aattgctacg gataaaaatg tacaaggtga taactatcgt acattgttac
     7621 catttaaaga aagccaggca agaggacttt tacaagataa catggcaaat agttataatg
     7681 gcggcgactt tgaagatggt ttattgaact taagtaaaga agtgtttcca acagacaaat
     7741 atttgtatca agatggtcaa tttttggaca agaaaacaat taatgcctat ttaaatccta
     7801 agtatacaaa acgtgaaatc gataaaatgt ctgaaaaaga taaaaaagac aagaaagcga
     7861 atgaaaattt aggacttaat ccatcacacg aaggtgaaac agatcctgaa aagattgcag
     7921 aaaaatcacc agcctattta tctaacattt tagagcaaga tttttatggt ggtggagata
     7981 caaaaggtaa gaatattaaa ggtatgacga ttggtttagc tatgaatagt gtttattact
     8041 ataaaaaaga aaaagatgga ccgactttta gtaaaaaact agatgatagc gaagttaaaa
     8101 agcaaggtaa acaaatggct agtgagatat tatcaaggtt acgtgaaaat gatgatttaa
     8161 aagatatacc aattcatttt gcaatttata agcaatcaag tgaagattca atcacaccag
     8221 gtgaatttat cactcaagcg actgcagaaa agagtcaaac aaagcttaat gaatggcata
     8281 atatcaatga aaaatcagct ttattacctt cttcaacagc agcagattat gatgaaaatt
     8341 taaataataa tttcaagcaa tttaatgata atttgcaatc atatttttct aatttcacac
     8401 aagcagtagg aaaagttaaa tttgttgata aaaagccaca acgattagta gtagatttac
     8461 caatcgatta ctatggacaa gctgaaacaa ttggtattac acagtacgtt actgaacaag
     8521 cgaataaata tttcgataaa atcgataact atgaaattcg gattaaagat ggtaaccaac
     8581 cacgtgcttt aattagtaag acaaaagatg acaaagaacc gcaagttcat atttacagta
     8641 attaattgta aattaaagat aataaaaagg taccaatgtt agtcataatt ttgattaaca
     8701 ttagtacctt tttaatttgt taagacttat gaattatttt tctctaacga tgtcacgaac
     8761 tttgtttaag tcagtttcaa caaatgcacc aggtttttta gtaagtttac ttacaacata
     8821 tgtaacgatt acacttacaa taaatccagg aataatttca tataagccga atatttcgtt
     8881 gatatgtgcc aatggtttaa tccacgcaat ccaaacgata acgactaagg cacctgaaac
     8941 cattccactt acagcaccgg cacgtgtcaa acctttccag taaagtgcaa atagcacaag
     9001 tggactgaac gatgcaccaa atccggccca agcattacct actaagttta gaattgtgtc
     9061 gtttggattc catgcaatcg cgatggcaac aattgctact gctaatacag ataaacgtcc
     9121 aaccataacg aattcttttt gatgtgtttt agctttttct tcaccacgaa ctaatttata
     9181 aaagtcttca gttaaagaac tagacgttac aagtaattgt gaagaaatgg tgctcataat
     9241 tgctgctaga atcgcagcaa gtaagaaacc acctacaaga ggatggaaga gtacttgact
     9301 catcacgatg aataatgtct caggatcttc tagtttaata tgataagcag gtacgaatgc
     9361 aatacctgtt aaaccaacag ccacagcgcc taataaacca acagccatcc agctaatacc
     9421 taaacgtcta gctttaggta gcatcttgtg tgatttaata gacataaacc ttacaatgat
     9481 atgaggttga ccgaaataac ctaatcccca tgaaaataga gagataattc ctataaatga
     9541 taacccttta aataaattta aatttgtagg tttcatagct gctacatcat gaaacgttcc
     9601 ccagccgttt aaattcatca tagcaacaat agggaccata accatcgcaa ttaacataat
     9661 gacaccttgg aagaaatctg taattgatac agctaaatat ccaccaaaga aagtatagaa
     9721 aatgacaatg aaagcaacta atattaaacc gaaatgataa tctaatccaa aagcactttc
     9781 aaataattta ccaccagata cgaaaccaga atgtgtatat aatgtaaaga atactacgat
     9841 aatcaatcca gaaataatct ttaacacatt atttttatcg tttaaacgat ttttaaagaa
     9901 atctggtaat gtaattgcat ctccagctaa ttcggtataa acgcgaagtc taggagcaac
     9961 aacaaagtaa tttatataag cacctaatgt taagccgatt gtaatccaca ttgccgaaag
    10021 accagtacta tagacagaac caggtagacc cataatcatc catccactca tatctgaagc
    10081 tccagctgat aatgcagtaa tatacggtcc aatactacgt ccgcctaaca tgtactcgct
    10141 taggttacca gttgcttgct tgtaaccgta aaagccaata acgattagta tcaagaagta
    10201 gacggcaatc ataatataag tttgccaatt ggcatctact tgttgactta atgctgtccc
    10261 cattgtaagc atgataaaca ctccctttat ttaatttata atttagttac ttgaaaacat
    10321 tatacataaa gataaatgtt tagaaaagta ggaaaattaa atttattctt tttgaaatac
    10381 ttgtatacca atatagaaaa cgctttcgta ttttttagat atgagcaaat ttttataatt
    10441 aaaattgcat aatcataata ttgaattggg ttgtctaata ataaaagggt gttgttttca
    10501 taaaaaatga tttggtaaac gaatgataat gtttatacag tttatatgaa attaaagtag
    10561 tacctcataa atacttagat ttttaattgg aaatttgata caatttagtg atgaatgact
    10621 taaaggaggc ttttattaat gacaaaagta acacgtgaag aagttgagca tatcgcgaat
    10681 cttgcaagac ttcaaatttc tcctgaagaa acggaagaaa tggccaacac attagaaagc
    10741 attttagatt ttgcaaaaca aaatgatagc gctgatacag aaggcgttga acctacatat
    10801 cacgttttag atttacaaaa cgttttacgt gaagataaag caattaaagg tattccgcaa
    10861 gaattagctt tgaaaaatgc caaagaaaca gaagatggac aatttaaagt gcctacaatc
    10921 atgaatgagg aggacgcgta agatgagcat tcgctacgaa tcggttgaga atttattaac
    10981 tttaattaaa gacaaaaaaa tcaaaccatc tgatgttgtt aaagatatat atgatgcaat
    11041 tgaagagact gatccaacaa ttaagtcttt tctagcgctg gataaagaaa atgcaattaa
    11101 aaaagcgcaa gaattggatg aattacaagc aaaagaccaa atggatggca aattatttgg
    11161 tattccaatg ggtataaaag ataacattat tacaaacgga ttagaaacaa catgtgcaag
    11221 taaaatgtta gaaggttttg tgccaattta cgaatctact gtaatggaaa aactacataa
    11281 tgagaatgcc gttttaatcg gtaaattaaa tatggatgag tttgcaatgg gtggttcaac
    11341 agaaacatct tatttcaaaa aaacagttaa cccatttgac cataaagcag taccaggtgg
    11401 ttcatcaggt ggatctgcag cagcagttgc agctggctta gtaccattta gcttaggttc
    11461 agacactggt ggttcaatta gacaaccggc tgcatattgt ggtgttgtcg gtatgaaacc
    11521 aacatacggt cgtgtatctc gatttggatt agttgcattt gcatcttcat tagaccaaat
    11581 tggtccattg actcgaaatg taaaagataa tgcaatcgta ttagaagcta tttctggtgc
    11641 agatgctaat gactctacaa gtgcacctgt tgatgatgta gactttacat ctgaaattgg
    11701 taaagatatt aaaggattaa aagttgcatt acctaaagaa tacttaggtg aaggtgtagc
    11761 tgatgacgta aaagaagcag ttcaaaacgc tgtagaaact ttaaaatctt taggtgctgt
    11821 cgttgaggaa gtatcattgc caaatactaa atttggtatt ccatcatatt acgtgattgc
    11881 atcatcagaa gcttcgtcaa acctttctcg ttttgacgga attcgttatg gttatcattc
    11941 taaagaagct cattcattag aagaattata taaaatgtca agatctgaag gtttcggtaa
    12001 agaagtaaaa cgtcgtattt tcttaggtac atttgcatta agttcaggtt actacgatgc
    12061 ttactataaa aaatctcaaa aagttagaac attgattaaa aatgactttg ataaagtatt
    12121 cgaaaattat gatgtagtag ttggtccaac agcgcctaca actgcgttta atttaggtga
    12181 agaaattgat gatccattaa caatgtatgc caatgattta ttaacaacac cagtaaactt
    12241 agctggatta cctggtattt ctgttccttg tggacaatca aatggccgac caatcggttt
    12301 acagttcatt ggtaaaccat tcgatgaaaa aacgttatat cgtgtcgctt atcaatatga
    12361 aacacaatac aatttacatg acgtttatga aaaattataa ggagtggaaa tcatgcattt
    12421 tgaaacagtt ataggacttg aagttcacgt agagttaaaa acggactcaa aaatgttttc
    12481 tccatcacca gcgcattttg gagcagaacc taactcaaat acaaatgtta tcgacttagc
    12541 atatccaggt gtcttaccag ttgttaataa gcgtgcagta gactgggcaa tgcgtgctgc
    12601 aatggcacta aatatggaaa tcgcaacaga atctaagttt gaccgtaaga actatttcta
    12661 tccagataat ccaaaggcat atcaaatttc tcaatttgat caaccaattg gtgaaaatgg
    12721 gtatatcgat atcgaagtcg acggtgaaac aaaacgaatc ggtattactc gtcttcacat
    12781 ggaagaagat gctggtaagt cgacgcataa aggtgagtat tcattagttg acttgaaccg
    12841 tcaaggtaca ccgctaattg aaatcgtatc tgaaccagat attcgttcac ctaaagaagc
    12901 atatgcatat ttagaaaaat tacgttcaat tattcaatac actggtgtat cagacgttaa
    12961 aatggaagag ggatctttac gctgtgatgc taacatctct ttgcgtccat atggtcaaga
    13021 aaaatttggt actaaagctg aattgaaaaa cttaaactca tttaactatg tacgtaaagg
    13081 tttagaatat gaagaaaaac gccaagaaga agaattgtta agtggtggag aaatcggaca
    13141 agaaacacgt cgatttgatg aatctacagg taaaacaatt ttaatgcgtg ttaaagaagg
    13201 ttctgatgat taccgttact tcccagagcc tgacattgta cctttatata ttgatgatgc
    13261 ttggaaagag cgtgttcgtc agacaattcc tgaattacca gatgagcgta aagctaagta
    13321 tgtaaatgaa ttaggcttac ctgcatacga tgcacacgta ttaacattga ctaaagaaat
    13381 gtcagatttc tttgaatcaa caattgaaca cggcgcagat gttaaattaa catctaactg
    13441 gttaatgggt ggcgtaaacg aatatttaaa taaaaatcaa gtagaattat tagatacaaa
    13501 attaacacca gaaaatttag caggtatgat taaacttatc gaagacggaa caatgagcag
    13561 taaaattgcg aagaaagtct tcccagagtt agcagctaaa ggtggtaatg ctaaacaaat
    13621 tatggaagat aatggcttag ttcaaatttc tgatgaagca acacttctaa aatttgtaaa
    13681 tgaagcatta gacaataacg aacaatcagt tgaagattac aaaaatggta aaggcaaagc
    13741 tatgggcttc ttagttggtc aaattatgaa agcgtctaaa ggtcaagcta atccacaatt
    13801 agtaaatcaa ctattaaaac aagaattaga taaaagataa ttagattcat cagactatga
    13861 tgatataaca atgaaccctt ggttgctgac ttagatgcaa tcgagggttt atttatatct
    13921 atagaagtca tattactttt aactttattc attgtacatg ttaatggtaa aaatattaat
    13981 tttattaatg cgttagcttt aattatatta aggcaaactg tataataaaa aggtataaaa
    14041 catttttgta taaagacaac attatattta caacatcatt ttaaaggtaa aatagcataa
    14101 ctgacgaagt ctatataacg aagaacggca aaaaatgctg aataaataac aacctttgta
    14161 catattgaga tagtatttgt ttaagataca agttggtctt taacgatatt aagaatgatg
    14221 agataagact gatcctgggc cataaattca atgtcctagg cactacaatg ttatattggc
    14281 agtagttgac tgaaagaaaa tacgcttgta ataagctttt ttcaattcta gtggggcccc
    14341 aacaaagaag ctgactttct gtcagcttac aataatgtgc aagttagggt ggggccccaa
    14401 caaagagaat ttcgaaagga aattctacag acaatgcaag ttggcggggc cccaacatag
    14461 aagctgactt tctgtcagct tacaataatg tgcaagttgg ggtggggccc caaacataga
    14521 gaattttgaa aagaaattct acagacaatg taagttgggg tagaacgaaa taaattttgt
    14581 taaatattat ttctgtccca ctcctattag acgaaaccaa gatgaagtta aaatatatga
    14641 attttaagta gagggataag atatgaggaa acgtgctaga atcatttata acccgacatc
    14701 aggtaaagag caatttaaaa gagaattacc tgatgcctta ataaaattag aaaaagcggg
    14761 atatgaaacg agtgcatatg caaccgagaa aataggtgat gccacacttg aagcagaaag
    14821 agctatgcat gaaaattatg atgtattaat cgctgcaggt ggtgacggaa cattaaatga
    14881 agtagttaat ggtatcgcag aaaagcctaa tcgtcctaag ctaggtgtca ttcctatggg
    14941 tactgttaat gactttggac gtgcattgca tatacctaat gacatcatgg gggcacttga
    15001 tgtcatcatt gaaggtcatt ctactaaagt agatattggt aaaatgaata atcgatactt
    15061 tattaattta gctgcaggcg gacaattgac gcaagtctct tatgaaacac cgagtaaatt
    15121 gaaatctatt gttggtccat ttgcttatta catcaaaggt ttcgaaatgt tacctcaaat
    15181 gaaagctgta gatttaagaa ttgaatatga tggtaatgtt ttccaaggag aagcattatt
    15241 attcttttta ggtttaacaa attcaatggc aggatttgaa aaattagtgc cggacgctaa
    15301 gttagatgac ggctatttta cgttaattat agttgaaaaa tcaaaccttg cagaacttgg
    15361 tcatattatg actttagctt caagaggaga acatactaag catcctaaag ttatttatga
    15421 aaaagcaaag gcaattaata tttcatcatt cacagactta caactaaatg tagatggaga
    15481 atatggtggt aaattaccag cgaatttctt gaatctagaa cgtcatatag atgtctttgc
    15541 accaaatgat attgtaaatg aagagttaat aaataatgat catgttgatg acaatttaat
    15601 cgaagaataa acagaagtag tgaaatatat aatggcaggt taggaagcgc ataattttgt
    15661 tcctaacctg ttcattttgg aggtaaaatt ttgcaagcaa tagctaaaaa tgacattaaa
    15721 acgggaacag ttgttgattt aacgcatgaa ggtcatggtg tcgtaaaaat agatcgcttt
    15781 cctattttca taccacaagc tttaattaat gaacaaattg aatataaaat tatcaaagta
    15841 aaaaagaact ttgcgatagg taagttgctt aatataaata cgagaagtga gaatcgtgtt
    15901 gcaccgccat gtatatatta tgaacgttgt ggtgggtgcc aacttcaaca cttatcatac
    15961 gaagcacaac ttgaaatgaa aaaggaacaa gtgattaatt tgtttcagcg aaaagcgcat
    16021 tttgataatt ctaagattaa cgatacagtt ggcatgacag atccttggag atatcgaaac
    16081 aagtcccaga ttccagttgg taagaatgaa cagaatgaag ttattatggg cttttatcgt
    16141 caaagaagcc atgacattat tgacatggga agttgtttaa tacaagattc acaacatcaa
    16201 gaagtgatga atgaagttaa atctatttta aaagatttaa atgtaagtat ttatcaagaa
    16261 caattgaaaa aaggtttaat gagacatttg gttgtaagaa caggatatca tacagacgaa
    16321 atgatggtta tttttgtaac taatggtaaa aattggccac aaaaaaatgc tgttgttgag
    16381 aagatattag atgcatttcc taatgtgaca agcattaaac aaaacattaa tgatagccac
    16441 tctaatgtta ttatgggacg tcagtctata acgctatatg gtaaagatac aattattgat
    16501 caattaacag atagtacatt taaaataagt gatcagtcat tctaccaaat taattctgaa
    16561 caaacagaga aattatataa taaagcaatt gagtatgcgc aattgacagg taatgaagtt
    16621 gtattagata cctattgtgg tattggaaca ataggactat atatggcacc acatgcaaaa
    16681 catgtatacg gtgttgaagt tgtaccttct gcaattgaag acgcacaaca aaatgcgact
    16741 ataaaccaat gcaataatac aacttttgtc tgtgggaaag ctgaagaagt tattttacaa
    16801 tggaaagcac aagggattaa accagatgtt gtaatggttg atccgccaag aaagggctgt
    16861 gacgaaacgt ttattcagac tttgcttaca ttagaaccta agcgtattgt ctatatttca
    16921 tgtaatccct caacacagca acgtgatgca ttattattag ctgaaaaata tcaacttgaa
    16981 gaggtaacac cggtagatat gtttccacaa acaacacatg ttgagacagt ggcattattc
    17041 aatttgaaat agcgcacatg atttgagata aaatttcgcc ttaataagtg aagaaagaag
    17101 tcgtatatta aaattttatt acagccaatt tactttcttg atatgaactt tttaaattaa
    17161 atgtgacatt gtatactata tttaaagaag aataagaatg tcatgatcgg gagggttggt
    17221 aatgcataaa attgatttaa cgacaaataa ttttcaaatg cgaaggttta ttatcttgca
    17281 acttgttatt gcgttattcg tgattttatt tacgtataag tgggcgttag gtgtcacagc
    17341 ggttgtcgat caaaatatca tcatcaatct tgtatatggg tttgctggat tcattatttt
    17401 attgatttta catgaactta tccatagagc attatttctg ttatttaaaa aagatagcaa
    17461 acctatgttt aatataaaaa aagacagaat cttattccaa acagcagatg cttgcttcaa
    17521 caaatggcaa ttctcaatta ttatgctgtc accactaatc ctattaagtg ctggcttgct
    17581 tattttaatt aaggtatttg gttattcatc acttattttt atgtttagta tgcataccgc
    17641 ttattgcttt atagatattc tgcttgttgc attaacaata tctagtagtt ttaaatatgt
    17701 tcaacaagat gaagatagta tttatttgta tcatcaaaag ccgactcaat aataaatttt
    17761 aatatgatga taccagcaaa atgaatttgc ttgaattatt tcagattacc tatgttttct
    17821 tactcaatcg tcttcatcat catgcttaaa tagcaattga ttttataaca acttgtagtt
    17881 atctcaatat agttatcgct aagtgcaaga tttatgtaaa aaaatcgtac acagcgtata
    17941 attatgttga gatatttttg tgcgttataa aagtaaaaat tagtagggag gtgagcactt
    18001 gactgagaga cgaattattc atatcgatat ggactatttt tttgcacaag tcgaaatgag
    18061 agataacccc aaattgaaag ggaaaccagt cattgttggt ggtaaagcaa gtagtagggg
    18121 cgttgtctct acggcatctt atgaagcacg aaaatacggc gtccattcag caatgcctat
    18181 gtcacaagcg cataaattat gtccaaatgg atactttgtc acaagtaatt ttggtgctta
    18241 ccgagaaaca tctgcgcaga ttatgtctat tttccgtagt tatacagata aggtagaacc
    18301 gatgtcatta gatgaagcgt atttagatat tacagaatta gtgagaccag accttcctgc
    18361 ttcgaaaatt gctcagtata ttagaaaaga tattctagag cagacacatt taacagcatc
    18421 tgcaggtgtt tcttataaca aatttttagc taaattagcg agtggtatga ataaacctga
    18481 tggtatgact gtgattgatt atcgaaatgt ccatgatatt ttgatgacat tggatattgg
    18541 agattttcca ggcgtaggta aagcttccaa aaaagtaatg catgataatg gtatttttaa
    18601 cggtagagat ttatatgaga aaacggaatt tgaattaata cgtttgtttg gaaaaagagg
    18661 tcggggttta tataacaagg cacgcggtat tgaccatagt gaagtgaaat caacaagagt
    18721 aagaaaatca gtagggactg aacgcacatt tgcaacagac gtgaatgatg atgaagagat
    18781 tttaagaaaa gtatgggagt tgtcaggtaa aacagctgaa cgtctaaata aattacagaa
    18841 gtcggctaaa actgtaacgg ttaaaattaa aacttatcaa tttgaaacgt tatctaaaca
    18901 gatgagttta agagattctg ttagttctga agaagatatt tataatattg catatttact
    18961 ttataacgat ttaaaagacc ctgatgtacc aattcgactt attggtgtca ctgtaggtaa
    19021 tttagaacaa tcaacttata aaaatatgac gatatatgac tttatataaa ataaagctcc
    19081 cttcaaagtt tacattttta caatgcttac tttgaaggga gtattttatt tagtcctagc
    19141 ctttatcttt tagattttta ccataaatat tagttacata tgttaagttt tcataatttt
    19201 tcagtagcct aaaagtaatc attgcgcatg ctttggcatc attcaatgca tcatgatgac
    19261 catgaaaatc taattgataa aactccatca tatgttttaa accgtatcga tacgaatcaa
    19321 cggttctttt agcaagttga taactacaaa agtaagttaa atttggagtt ggtaaaccaa
    19381 tattttgaat gctttgatgt aagacgttca tatcaaatgc cgcgttatgt gcgacaacag
    19441 gtaaatctgc aataaattgc atcatatatg gaaatacgta gtcaaacgta ggtgcatttt
    19501 caacatcttc tggttgtatg ccatgaactt taatattttg ttgtgaaaaa tagtcttgcg
    19561 gattcacaag agtatgaaat gtttctgtta tttgactatc aatgacttta accattccga
    19621 cagaacaaat acttgtacgt ttaccatttg ctgtttcaaa atcaagtgca acaaacgcat
    19681 cttgtatcat gtagtcaatc ctttcaatta attaaatgta tatattaata aactttttag
    19741 tcatttgctt acaaacgata cataatatta tacgtgaagg tataacgtga aatctatcat
    19801 ttggatgtaa ctctgattaa aaagtaaatg atgatatgct ttaaaatcgg tcaaattaat
    19861 acaacattaa atttagagtt attaacatta gtagtttaaa aaacaaatga ctaaaaatac
    19921 aataattagc cttgaaaatg aaaaaacgca gatcaatgat tcagaaaatg aatctagtga
    19981 tctccgtaaa gcaaaataat atagacattg ggttattgta agtgacccaa atgtctatca
    20041 tgtcgtatga tatttatctg tctattgtag tgatgtttaa ttattcttcg tcgaatgtac
    20101 gagtgccaag ttctttttcg taaagatata atgcattgct gtcatcgccg atacgagtta
    20161 aataattgat gtgagtttca aacattgctt cttcttcgac ttgttcatct aagaaccagt
    20221 ttaagaatga gatagttgca taatctttat cttgacgagc gatttcagat aagttataga
    20281 aacgtcttgt tacttcttgt tcttgagata agctgtcttt gaaagtttct agtatgcttg
    20341 aaaagtcaat ttttggtgct gaaactgctc tgaattctgc atgtgcacct ctgtcgttaa
    20401 tatagttata aatcttttgt ccatggaaac gttcttcttt agcttgttga atgaagaagt
    20461 ttgcaaatcc ttcatacgat tctttatcac agtatgctgc cattgccata tatgcgtgcg
    20521 ctgcaaagta ctcatggttc atttgatcat ttaatgcttc taataaattt ttacttaaca
    20581 ttttgataca cctcttattt gtaaattaaa attactatg
//
LOCUS       DAKSQE010000041        20494 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.041, whole genome shotgun sequence.
ACCESSION   DAKSQE010000041 DAKSQE010000000
VERSION     DAKSQE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20494
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.041"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..830)
                     /gene="dat"
                     /locus_tag="O0V33_001273"
     CDS             complement(<1..830)
                     /gene="dat"
                     /locus_tag="O0V33_001273"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HCX8975914.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYN
                     IPFKEETFTVDFLRNADEVIVSSTSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKY
                     "
     gene            complement(834..2243)
                     /gene="sapep"
                     /locus_tag="O0V33_001274"
     CDS             complement(834..2243)
                     /gene="sapep"
                     /locus_tag="O0V33_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HCX8975915.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLAEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAKAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
     gene            complement(2449..2871)
                     /locus_tag="O0V33_001275"
     CDS             complement(2449..2871)
                     /locus_tag="O0V33_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCX8975916.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFEEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
     gene            complement(2888..3583)
                     /locus_tag="O0V33_001276"
     CDS             complement(2888..3583)
                     /locus_tag="O0V33_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCX8975917.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKISVRGELIEYVENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEEDIQAFKEGITLSDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            complement(3580..5241)
                     /locus_tag="O0V33_001277"
     CDS             complement(3580..5241)
                     /locus_tag="O0V33_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCX8975918.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNVHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVAIKLALNYPLIMLFHTPGAVLSTSIA
                     LLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVEAVFFIANLFLEPTKLG
                     YLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR"
     gene            5645..6914
                     /locus_tag="O0V33_001278"
                     /pseudo
     CDS             5645..6914
                     /locus_tag="O0V33_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
     gene            complement(7031..13600)
                     /gene="sasC"
                     /locus_tag="O0V33_001279"
     CDS             complement(7031..13600)
                     /gene="sasC"
                     /locus_tag="O0V33_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HCX8975919.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDHNV
                     QGGSNQALPGNSQNTNADTNRDIVNDSQNTPNAHATDNTSTNQALTNHQNVDVANQVG
                     PAPIQPSASPAQNNNNSNANSTATEPAANTNNNLASNNNTLNVPNNTDNNDSARHLTL
                     KEIQEDVRHSSDKPELVAIAEEASNRPKKRSRRAAPTDPNATPADPTATPADPTAGNG
                     SAPVAITAPYTPTTDPNANNIGQNAPNEVLSFDDNNIRPSTNRSVPTVTVVDNLPGYT
                     LINGGKVGVFSHAMVRTSMFDSGDAKNYQAQGNVIALGRIRGNDTNDHGDFNGIEKTL
                     TVNPNSELIFEFNTMTTKNYQGMTNLIIKNADNDTVIGEKVVAYGPIWRLLKVPENVS
                     HLKIQFVPKNDAITDARGIYQLRDGYKYYDFVDSIGLHSGSHVYVERRTMEPTATNNK
                     EFTVTTSLKNNGNFGASFNTDDFVYKIQLPEGVEYVNNSLTKDFPSGNSGVDINDMNV
                     TYDAANRIITIKSTGGGTGNSPARLMPDKILDLKYKLRVNNVPTPRTVTFNDTLTYKT
                     YSQDFINSPAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNDLKRR
                     AQTILDENRNNVPLNKRVSQADIDSLANQMQHTLIRSVDAENAVNRKVDDMEDLVNQN
                     DELTDEEKQAAIQVIEEHKNEIIGNIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPN
                     AKQAIRDKAAKQREIINHTPDATQDEIQDALNQLTTDETDAIDNVTNATTNADVETAK
                     NNGINTIGAVAPQVTHKQAARDAINQATATKRQQINSNREATQEEKNAALNELTQATN
                     HALEQINQATTNDDVDTAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANP
                     DATQEERQAAIEKVNAAVAVANTNILNANTNADVEQVKTNAIQGIQAIEPATKVKTDA
                     KNAIDQSAETQHNAIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKD
                     QGTQNIVVIQPATQVKTDARNAVNEKAREAITNINATPGATREEKQEAINRVNTLKNR
                     ALNDIGVTSTTAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLTDLATAKKQEINQNTN
                     ATTEEKQVALNQVDQDLATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKPAAL
                     AQTNQHYSAKLVEINATPDATDDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATV
                     AENNIDAVQVDVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQA
                     FNQINQNQTNDQVDATTNQAINAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSLDS
                     TDNEKEVALQALAKEKEKALAAIDQAQTNSQVNQAATNGVSAIKIIQPETKIKPAARE
                     KINQKANELRAQINQDKEATAEERQAALDKINDLVAKAMTNITNDRTNQQVNDSTNQA
                     LDDIALVTPDHIVRAAARDAVKQQYEAKKHEIEQAEHATDEEKQVALNQLANNEKRAL
                     QNINQAIANNDVKRVESNGIATLKGVEPHIVVKPEAQEAIKASADNQVESIKDTPHAT
                     TDELDEANQQINDTLKQGQQDIDNTTQDAAVNDVRNQTIKAIEQIKPKVRRKRAALDN
                     IDESNNNQLDAIRNTLDTTQDERNVAIAALNKIVNAIKNDIAQNKTNAEVDQTEADGN
                     NNIKVILPKVQVKPAARQSVSAKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAID
                     QINHADKTAQVNQNSIDAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATD
                     EEQNAAIVQVEKELIKAKQQIAGAVTNADVAYLLHDGKNEIREIEPVINKKATAREQL
                     TTLFNDKKQAIEANVQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTATLNLQ
                     TIHDLDVHPIKKPDAEKTINDDLARVTHLVQNYRKVSDRNKADALKAITALKLQMDEE
                     LKTARTNADVDAVLKRFNVALGDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQL
                     AKVKALIDQYVADGNRMVDEDATLNDIKKDTQLIIDEILAIKLPAEVIKASPKVGQPA
                     PKVCTPIKKEDKQEVRKVVKELPNTGSEEMDLPLKELALITGAALLARRRSKKEKES"
     gene            complement(13924..14235)
                     /locus_tag="O0V33_001280"
     CDS             complement(13924..14235)
                     /locus_tag="O0V33_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCX8975920.1"
                     /translation="MKSITTDELKTKLLESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLNAFNKKETYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
     gene            complement(14257..16674)
                     /locus_tag="O0V33_001281"
     CDS             complement(14257..16674)
                     /locus_tag="O0V33_001281"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCX8975921.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNSEGKVEVFTTRPDTIYGASFLVLSPEHALVDSITTDEYK
                     DQVKAYQTEASKKSDLERTDLAKDKSGVFTGAYAINPLSGEKVQIWIADYVLSTYGTG
                     AIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAI
                     TKAIQLLEKKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLL
                     PETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPK
                     NENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLGIVPTKEPFQKLFN
                     QGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGS
                     RRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLM
                     VFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALL
                     VDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQK
                     LVNIVAK"
     gene            complement(16963..18144)
                     /locus_tag="O0V33_001282"
     CDS             complement(16963..18144)
                     /locus_tag="O0V33_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCX8975922.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPILYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMVLLVFALILLMVFKENNTQPKKID
                     A"
     gene            18254..19207
                     /locus_tag="O0V33_001283"
     CDS             18254..19207
                     /locus_tag="O0V33_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCX8975923.1"
                     /translation="MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMQTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            19204..19767
                     /locus_tag="O0V33_001284"
     CDS             19204..19767
                     /locus_tag="O0V33_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCX8975924.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLVKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINALLSLMSTEGIIVLVIYHGHSEGQLEKQALLDYLSTLEQ
                     KHAQVLKYQFLNQRNHAPFICAIEKIS"
     gene            complement(19886..20287)
                     /locus_tag="O0V33_001285"
     CDS             complement(19886..20287)
                     /locus_tag="O0V33_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCX8975925.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
BASE COUNT         6123 a         3816 c         3113 g         7442 t
ORIGIN      
        1 atatactttt caaatccttc ttgtaattga cgtgtaattg ggccaacttt accatcatta
       61 actggttcac catctaattt aataacaggt gtaacctcag ctgaagtact tgaaacaata
      121 acttcatctg cgtttctcaa gaaatctaca gtaaacgttt cttctttaaa tgggatgtta
      181 tagtcttcgg caattttttt aattacaatt cgggtaatac cattaagaat atagttgtta
      241 atcggatgtg tataaatcac accgtcttta attgcataag cattacttga agatccttca
      301 gtgacagttt cgcctcgatg ttggattgct tcaactgcat tatatttcac agcatattct
      361 tttgctaata catttcctaa taggttcaag cttttaatgt cgcaacgtaa ccatcggata
      421 tcttcaacgg taacaccatt cacaccattt tctaaatgat cataaggacg atcataactc
      481 ttagtataag caacaattgc tggttctact tcaggtgtcg ggaagctatg atttctttca
      541 gctacacctc gcgttgcttg aatataaatt gccccagttt caatttgatt catatcaact
      601 aatttacgag atagttcaat taattcttct acagaataat ttaaatctaa accaatctca
      661 ttggcactac gtaaaaatct ttcataatgt tctgttactg taaataactt accattgtat
      721 actcgaatgt attcataaat accatcgcca aatacgtatc ctctgtcgtt gtatgaaacc
      781 tttgcttcac ttggacttac aaactcacca tttaaaaaaa ttttttccat atattattcc
      841 tccacgcata atgaataaat tgcttctaag taaatactag ttgcgttaaa taactgtttt
      901 ttagtgatat attcattttt ctgatgcatt aaatcttcag aatcactaaa cattgcgcca
      961 aatgctacac ccttgtctaa gtttctcgca taagtaccgc cacctatagt ataaggttca
     1021 gtcatatcat ttgtttgatt tctatatgca gtaactaact tttgtacaaa aggatcattt
     1081 ttatcaacat aatgtggtgg ttggacttta cctaatttca cttcaaagcc atattgttga
     1141 atctcatttg caaaacgatc catagctttt tcaaattcaa atccttctgg gtagcgcaag
     1201 ttgataccga aaagacccgc attttcatta tcatatgtaa taacaccaat gttagttgtc
     1261 acatcaccca tgacatctgt atggaatttc attcccatct tttcaccaaa atctgaatta
     1321 aataagtagc gattactaaa tgctacaaac gcttttgcat tattatcaag atttaatgat
     1381 gctaagaatt ttagtaagta aagacccgca ttcacaccga tagatgggtc cataccatga
     1441 accgctttac cttcaactgt taaaactaga atgccactat caacagtact atcaccttgt
     1501 aaatgatttt gttctaaaaa atactcaaag tcttgaataa catctgtcat attttcttta
     1561 acaagcactc ttgcttctgc atgatcaggt accatgttgt aacgttcacc agatttaaaa
     1621 gttattaatt cataatcagg ttcatcttga tcttcagcaa gcttattttg aactaagtca
     1681 aatgttgtaa tgcctttttc accatgaata cacggaaatt ctgcatctgg tgcaaaacct
     1741 aatgttggca tttcttctgt tttaaaatag cgatccgtac atttccaatc agattcttca
     1801 tccgtaccaa taatcatatg aatacgtttc ttccaatcca cattcatatc ttctaatatc
     1861 ttaattgcat aataagcagc aattgttgga cctttgtcat caagtgtacc tctagctatg
     1921 atagcatctt ctgttacaac cggctcaaac ggattactat cccatccatc accagcagga
     1981 acaacgtcaa catgacataa gatacctaat acgtcatttc ctttacctgc ctcaattctt
     2041 cctgcaatat gatccacatc atgtgttgta aatccatctc tatgtgcaat ttcatacatg
     2101 tagtctaatg ccttacgagg acctggacca actggtgcgt cttctgatgc ttttgcatca
     2161 tctctcacac tttcaattgc taataatcct tttaagtcat taatgatttg atcttcgtat
     2221 tgttgaactt tttctttcca cattcgaaat cgacttcctt ttttctataa gttaaatcct
     2281 attttacatg aaatgatata aaaactacaa taagatgtca gaaaataata aaaaggaaca
     2341 aaacaacgct atcgatttaa cataaaatta atgataactg ctttgttcct tttctgtatt
     2401 aatttaattt aatatgccag ttcaatacca acttattaat tgtatgcctt attctttatc
     2461 agacaatgca tttttattct tttttaaatc ttcttcagtg acgatacgta aattattatt
     2521 tggtgctgcg ccaccttcat catcaaattt acctttttca atactttcgt cagtcttatt
     2581 gtcatattcg gtaaattttg atttttcttc ttcgaaaaat gcttttggat tattttttaa
     2641 tctattagca tattctttcg gatttgtttt tacttcttta attgtttcat ttgcaattgt
     2701 tcctaattgc gtcgctttat ccttagcatt atctttatag ctttgaggat cttgtttgta
     2761 tttattatat tctgctttca gcttgtcacg actatcttta cgtgtaacaa gtacagctgc
     2821 tacagcgcca cctataccta aaatcgcttt aaataaatta ccttttgcca tatcaatcgt
     2881 ctccctttta tttataattt aatttgtcaa aatcattttc agttaataaa cgatattctc
     2941 ctgaatctaa attgctatcc aattctaaat cagcaatttt gatacgtctt aaatgtaata
     3001 cctcattttg aatgctatga aacattcgtt taacttgatg atattttcct tcataaattg
     3061 ttacgtgtga cgtttgatta tcaatataag ttaatattgc aggcttaacc ttgccatcag
     3121 acagtgttat accctcttta aaagcttgaa tgtcttcttc agtgatagga tttgctgaaa
     3181 taacttcata ttttttagaa acatgtttgt ttggactcat taattcatga ttaaaatcac
     3241 catcattcgt tatcaataaa agcccttctg tatctttatc aagacgacca accggaaaaa
     3301 tatttaaatg ttggtattca ggtattaaat caataacggt ttttgaatga tgatcttcag
     3361 ttgccgatat ataacctttt ggcttattta acataatata gacattttca acgtattcta
     3421 ttaattctcc acgaactgat atcttatcgt tttctggttc tatatgtgtt tttggtgatt
     3481 taattacttg ttcgttgaca tttacaaggc cttttttaag taactgtttg acctcattac
     3541 gtgttccgac gcccatattt gctaaaaatt tatctattct catcgtaaaa acctaactct
     3601 acgtcttaat ttttcaggaa tttcacctaa gaattcgtcc gcaagacgcg ttttaattgt
     3661 gattgtaccg taaattagaa taccaactgt tacacctaaa atgataataa ttaaatatcc
     3721 aagttttgtt ggctctaaga ataggttcgc aatgaagaat actgcttcta cacctaacat
     3781 cataataaat gagtataaga atatttttgc gaaatgaatc cagctatagc taaatttaaa
     3841 tttcgcgtat tttttaagaa tatagaagtt acaaccaatt gcgaataata atgcgatact
     3901 tgtgcttaaa acagcccccg gcgtatggaa tagcataatt aatgggtagt ttaatgctaa
     3961 tttaattgca acagaagcta aaataacata aaccgttaac ttctgcttat cgataccttg
     4021 taacattgac gccgttacac ttaatagtga aattaatatt gctacaggcg cataatagaa
     4081 tagcaaacga ctaccatcat ggttagggtc atgacctaaa acaattggat cgtaaccata
     4141 gaatactgtg aataacggtt gtgccaaggc cataattcca atactagctg gaacagttat
     4201 aaacattaat acaccaatag atgttctaat ttgatgatgc atttcatgta agcgaccttc
     4261 tgcaaatgtt tttgtaatat aaggaattaa acttactgca aagccagcac ttaatgatgt
     4321 cggaatcatt acaattttat tagttgacat atttagcata ttaaagaata tatcttgtaa
     4381 ctgtgaaggt ataccaacta aagataaagc accgttatgt gtaaattgat ctactaagtt
     4441 aaataatgga taattcaaac ttacaataac gaacggaata ctataagcaa taatttcttt
     4501 atacatcttg ccatatgaca catctatatc tgtgtaatca gattcgacca tacgatcaat
     4561 attatgctta cgctttctcc aataatacca gagtgtgaat ataccaataa tcgcaccaac
     4621 tgctgctgca aaagtagcaa taccattggc taataagata gaaccatcaa agacatttag
     4681 tactaaataa cttccgatta atatgaaaat cacgcgtgca atttgctcag ttacttccga
     4741 taccgcagta ggtcccatag atttataacc ttggaatata cctctccatg ttgctaatac
     4801 aggaatgaag atgactacca tactaatgat tctaataatc catgtaatgt catcaactga
     4861 ccaaccattt ttatcgtgaa catttctagc taaagttaat tcagaaatat atggtgctag
     4921 gaaataaagt actaagaaac ctaaaacacc ggtaatactc atgacaataa aactagattt
     4981 ataaaatttc tgactgactt tatatgcacc aatcgcatta tacttcgcta catatttaga
     5041 agcagctaat ggtacacctg ctgtcgcaac tgcaattgca atattatatg gtgcataagc
     5101 gtatgtgaac ggcgccatat tttcttgtcc accaattaaa tagttgaatg gaatgataaa
     5161 aagtacgcct aacacctttg taatcaaaat actaattgta attaaaaagg tcccacgcac
     5221 catttcttta ctttcactca ttacgtatct ccctatctca tgtttattaa tgttttgtaa
     5281 ataaaaagct atttctctgt aaaatcattt tcattattat gaatatatca caaaacttta
     5341 tttcattgtc gtatattcaa tgaaatatca taacaaaaat aataacgtgt tgtcactgaa
     5401 tactcaaagt tgctttgaaa ttcataaatt ttcatattgt cattatacta ctaatgccaa
     5461 taaatggcgg acaactctta aaataataat agcgttcgaa taaatgctta tccttttatg
     5521 aaacacacat tataaatata ttcagcgcga gtgtcatttc ataacacaag agatatagca
     5581 aagtttaaaa aagtactata aaatagcaat tgaatgtcca gtaacaaatt tggaggaagc
     5641 gtatatgtat caaacaatta ttatcggagg cggacctagc ggcttaatgg cggcagtagc
     5701 tgcaagcgaa caaaatgaca acgttctact catcgaaaaa aaagaaaggt ctaggtcgta
     5761 aactcaaaat atctggtggc ggtagatgta acgtaactaa tcgattaccc tatgctgaaa
     5821 ttattaagaa cattcctgga aatgggaaat ttttatatag tcccttttca attattgata
     5881 atgaatccat catagatttt tttgagtcta ggggtgttaa attaaaagaa gaagatcacg
     5941 gtcgtatgtt tccagtttcc aataaggcac aagacgtcgt tgatacttta gtgacaacta
     6001 tagaaaatca acatgtaacg attaaagaag aagaagctgt tagtagaatc gaagttaata
     6061 cagaccaaac tttcactgta catactcaaa ataatagtta tgaaagccat tcgctagtaa
     6121 ttgctacagg tggtacaagt gtccctcaaa ctggttcaac aggtgatggt tataagtttg
     6181 cacaagattt aggtcatacc attacggagt tattcccgac cgaagttcca attacatcag
     6241 ctgaaccttt cattaaatcc aatcgtctaa aaggcttaag tttaaaagat gttgaattgt
     6301 cagtacttaa gaaaaatggt aaaaaacgca tcagtcatca aatggatatg ttatttactc
     6361 attttggtat cagtggtcca gctgcattaa gatgtagtca gtttgtttat aaagaacaaa
     6421 aaaatcaaaa gacacagcat atttcaatgg caatcgatac atttcctgaa ttaaaccatg
     6481 aacaattaaa acaacacatc acatcattat tatcggacac accagataaa atcattaaaa
     6541 acagcttgca tggactaatt gaagagcgct acttattgtt catgctggaa caagctgaaa
     6601 tcgatgaaaa taccacatca catcacttat caaatcaaca attgaacgac ttagtaaata
     6661 tgtttaaagg gtttgtattt aaggtaaacg ggacattacc tatagataag gcatttgtca
     6721 caggtggtgg tgtgtcacta aaagaaattc aacctaaaac catgatgtct aaattagttc
     6781 ctggattatt tttatgtggt gaagtattag acatacatgg ttatactggt ggttataata
     6841 ttacaagtgc actcgtaaca ggacatgtcg ctggattata tgccggtcat tactcacatg
     6901 catcaatgga ataatagtat aaaattgggt tcgattctct ctagtagatc aaccttttca
     6961 ttcaattaaa aatgacctta atataactga gtcactaaaa agtgtcgtta tattaaggtc
     7021 tttttgttta ttatgattct ttttcttttt tagaacgtct tctagctaat aatgctgcgc
     7081 ctgtaattag tgctaattct tttaatggta aatccatttc ttcagaacca gtatttggaa
     7141 gttcttttac aacttttcgc acttcttgtt tatcttcttt tttaataggc gtacaaactt
     7201 ttggagcagg ttgccccact tttggtgacg cttttatcac ttcagcaggt aatttaattg
     7261 ctaaaatttc atcaataatg agttgcgtat cttttttgat gtcatttaat gtcgcatctt
     7321 catcaaccat tctattgcca tctgcaacat attgatcaat taatgctttt actttagcta
     7381 attgttctgg tgttgcgatc gctttgaatt tcgcatatgt ttgttgagca atgttatcaa
     7441 tgcgcagtaa gctattttct ttttcagtaa ttactgcttc tatatcgcct aatgcaacat
     7501 taaatcgttt taaaactgca tcaacatcag cattagtgcg tgctgttttt aattcttcat
     7561 ccatttgtaa ttttaatgca gttatagctt ttaatgcatc agccttatta cgatcactta
     7621 cttttcgata attttgcact aaatgtgtaa cacgtgcaag atcatcatta atcgtttttt
     7681 cagcatctgg ctttttaata ggatgtacgt ctaaatcatg tattgtttgt agatttaatg
     7741 ttgctgtttt atcaacttgt gcattgctac gatcttgatc aatttgtcca atagcagtgt
     7801 cataaatgtt ttgtaactgt gctaaaatac tatttctttc ttctactgtt gcttgaacat
     7861 tcgcttcaat tgcttgtttc ttatcgttga ataatgttgt taattgttct cgcgcagttg
     7921 cttttttatt aataacaggt tcgatttcac gaatttcgtt tttcccatca tgcaataaat
     7981 atgccacatc agcattagtc actgcaccag caatttgttg tttagcttta attaactctt
     8041 tttcaacttg tactattgca gcattttgtt cttcatctgt cgcttcgtta tttgctttaa
     8101 ttaaattaat tttatttgta gcgatatttt gaatttgttg taatgctgtt gctttaactg
     8161 ttgtcgctgg tttaattttt gaaataatat tttgagcatc gatactattt tgattaactt
     8221 gcgcagtctt atctgcgtga ttgatctgat caatagcttg attaagtgct tgttctacta
     8281 aatgtttagc agctaatctt tcttcttcgg tagataaatc actttgatca ataagtgcat
     8341 tttgagcttc agcttttgcg ctgacagatt gacgcgctgc tggtttaact tgaactttag
     8401 gtaaaatcac tttgatgttg ttgttaccat cagcctcagt ttgatccact tctgcattcg
     8461 ttttgttttg tgcaatatca tttttaattg cattaacaat tttatttaac gcagcaatag
     8521 caacatttcg ttcatcttgc gttgtatcta gcgtatttcg tattgcatcg agttgattat
     8581 tattactttc atcaatgtta tccaacgctg cacgtttgcg tctaactttc ggtttaattt
     8641 gttcgattgc cttaatcgtt tggtttctaa catcattgac agctgcatct tgtgtcgtat
     8701 tgtctatatc ttgttgacct tgtttaagtg tgtcgtttat ttgttggttt gcttcatcta
     8761 attcatctgt cgtagcatgt ggtgtatctt ttatagattc tacttggtta tctgcgctcg
     8821 cttttatggc ttcttgagct tcaggtttaa ccacaatgtg cggttctacg ccttttaacg
     8881 tagcaatacc atttgattca acacgtttca catcattatt cgctattgct tgattaatgt
     8941 tttgtaatgc acgtttttca ttattcgcta attgatttaa agcaacttgt ttttcttcat
     9001 cagtcgcatg ttccgcttgc tcaatttcgt gctttttagc ttcatattgt tgcttaactg
     9061 catctctagc agctgctcta acaatatggt caggcgtcac taatgcaatg tcgtcaagcg
     9121 cttgatttgt tgagtcatta acttgctgat ttgttctatc attcgtgata tttgtcatag
     9181 ctttagcaac taaatcattg attttatcta acgccgcttg tctttcttct gctgtcgctt
     9241 ctttatcttg attaatttgc gcacgtaatt cattcgcttt ttgattgatt ttttcacgtg
     9301 ctgctggttt aatttttgtt tcaggttgaa taattttaat cgctgataca ccatttgttg
     9361 ccgcttgatt cacctgacta ttcgtttgag cttggtcaat agctgcaagt gctttttctt
     9421 tttctttagc taatgcttgt aaagcaactt ctttctcatt atctgttgaa tcaagactat
     9481 tatcaatttg ctgttgcttt tctttaacag ctttttcaat atctgcaatt gcctttggtt
     9541 taattactac ttcagcttca acattatcta tagcattaat cgcttgattt gtagttgcgt
     9601 ctacctgatc atttgtttgg ttttgattaa tttgattaaa cgcttgatct ttaagttgat
     9661 tgatttgatt aactgcagcc tgcttttctt cgtcagttgc attaggtgtt tgtttaaccg
     9721 cttcaatacg cttcgctact tcagcagtga ttttatctcg cgctgcttgt ttttttacaa
     9781 cgtcaacttg aacagcatcg atattattct ctgccactgt cgcagcttgg tctacttccg
     9841 catttgtatt tgcttgttta atactttcaa tagcttgttg tctgtcttga tttaaagtat
     9901 tgatcgcagc atttttctca tcatctgttg catctggtgt agcattgatt tcaactaatt
     9961 tagcactata atgctgattg gtttgtgcta atgctgcagg tttttttaca atatttggct
    10021 gaatcgcatt aattgctttt gtacctaatt gttgtgcttg atctacttct gcattagtat
    10081 cagcttgatt tatattatta attgccgttg ctaaatcttg gtctacttga tttaatgcta
    10141 cttgcttttc ttcagtggtt gcatttgtat tttgattaat ttcttgtttt tttgcagttg
    10201 ctaagtccgt taatacacct gtagcagttt gtttcttcgt tacatgcggt tgaactgcac
    10261 cgatttgatt gactgcatcg tctctaatac tattgaccat cgcagtagta gacgtcacac
    10321 caatatcatt taatgctcta tttttaagtg tattgacacg atttatcgct tcttgtttct
    10381 cttctcgagt cgcgccaggt gtagcattga tatttgttat cgcctctcgc gctttttcat
    10441 ttacagcatt gcgtgcatcc gttttaactt gtgttgccgg ttgaatcaca actatatttt
    10501 gtgtgccctg atcttttgct tgtgcaactt cttgattcgt atctgctgca ttaatatttt
    10561 gcttcgctgt ggctacagct tgatcaagca attgttgtgc tgcttgttgc tcttctaagg
    10621 tcgcatcatt attattaaat atcgcattat gttgcgtttc cgcactttga tcaatagcgt
    10681 ttttagcatc tgttttaacc tttgtagctg gttcaatggc ttgtatacct tgaattgcat
    10741 ttgtctttac ttgctcaaca tcagcattgg tattagcatt taatatatta gtattcgcaa
    10801 cagctacagc agcatttact ttctctattg ctgcttgtct ttcttcttga gtcgcatcag
    10861 gatttgcatt gatctctgcg atatgctgtt gtgcatcatg tgatacggca tctcttgctg
    10921 cttgcttgac aacagttaca ggcgcaatag gattaatggc attcagacca tcacctttgg
    10981 cagtatctac atcatcattg gttgtcgctt gattgatttg ttctaatgcg tggttcgtgg
    11041 cttgcgttaa ttcattcaat gctgcatttt tctcttcttg tgttgcttct ctattgctat
    11101 ttatttgttg tcgtttcgtt gctgtcgctt gattaattgc atctcttgca gcttgtttgt
    11161 gtgtcacttg tggcgcaact gcaccaattg tattaatacc attattttta gctgtttcaa
    11221 catcagcatt ggtagtagca ttcgtaacat tatcaatagc atctgtttca tccgttgtta
    11281 attgatttaa tgcatcttga atttcatctt gagtagcatc tggcgtgtga ttgataattt
    11341 ctctttgttt cgctgcttta tcacgtatag cttgtttagc atttggttta acaactggtg
    11401 ttgcagtgtc tccacttaaa gtctgtatac cttgatcttt aattctagta acgccatcat
    11461 cagtcgtttg gtcaccaata ttcccaataa tttcattttt atgttcctcg atgacttgaa
    11521 tcgctgcttg tttttcttca tctgtcagtt catcattttg gttaactaaa tcttccatgt
    11581 catcaacttt tctattaacg gcattttcag cgtcaacact gcgaattaac gtatgttgca
    11641 tctgatttgc taatgaatcg atatctgctt gagaaactct tttgtttaaa ggtacattgt
    11701 tacggttttc atctaaaatt gtttgtgcgc gtcttttaag atcattaaaa atatctaatg
    11761 atgcaaatgt ataatccgct tgttgaattc gtctatcgac ttcggcttgc aatgcgtctt
    11821 tattcatgat gatatcaatt gtatatggat ttgtacttac agtatgactt tcagcaggtg
    11881 aattaataaa atcttgtgaa tatgttttat acgttaatgt atcgttaaat gttactgttc
    11941 ttggtgttgg cacattgttc acacgtagct tatacttcaa atccaatatt ttatcaggca
    12001 ttagtcgtgc cggcgaattc cctgtacctc caccagtact tttaattgta ataattcgat
    12061 ttgctgcgtc atacgtcaca ttcatatcat taatatcaac acctgaatta ccgctaggaa
    12121 aatctttagt caatgaatta tttacatatt caacaccttc aggtaattga attttatata
    12181 caaaatcatc tgtattgaat gaagcgccaa agttaccatt attctttaat gacgttgtaa
    12241 ctgtaaattc tttattattt gttgctgttg gctccattgt acgtctttca acatagacat
    12301 gtgacccaga atgaagaccg attgagtcta caaagtcata gtatttatat ccatctcgta
    12361 attgataaat accacgtgca tctgttattg cgtcattttt aggtacaaat tgaattttta
    12421 gatgactaac attttcaggt acttttaata agcgccaaat cggaccataa gcaactactt
    12481 tttcaccaat aacagtatcg ttatcagcat ttttaatgat taaatttgtc ataccttgat
    12541 agtttttagt agtcatagta ttaaattcaa agattaattc agaattcgga tttactgtta
    12601 atgttttctc gataccatta aaatcgccat gatcatttgt atcatttcct ctaatacgac
    12661 ccaatgcaat tacattgcct tgcgcttgat agttcttggc atctcctgaa tcaaacatgc
    12721 tcgttcttac cattgcatga ctaaacaccc ctactttacc accattaatc agtgtgtagc
    12781 ctggtaaatt atcaacaaca gttactgtag gcacagaacg gttcgtactt ggtctaatgt
    12841 tgttatcatc aaatgaaagc acttcgttag gtgcattttg tcctatatta ttggcattgg
    12901 gatcagttgt tggcgtgtat ggcgctgtaa ttgcaactgg tgcactaccg tttcctgccg
    12961 ttggatctgc tggtgtagcc gttggatctg ctggtgttgc attaggatct gttggcgcag
    13021 cacgtctgct tctctttttc ggtctattag atgcttcttc agcaatcgca actaactctg
    13081 gcttatcaga cgaatgacga acatcttctt gaatttcttt taaagtcaga tgacgcgctg
    13141 aatcattgtt atctgtatta ttaggcacgt ttaatgtatt gttatttgat gctaaattat
    13201 tatttgtatt cgccgctggc tctgttgctg ttgaattagc attagaatta ttattatttt
    13261 gcgcaggcga cgcgctaggc tgtattggag caggcccgac ttgatttgcc acatcaacgt
    13321 tttgatgatt agtcaatgct tgatttgttg atgtattgtc tgttgcatgt gcattaggag
    13381 tattttgcga atcatttact atgtctcgat tagtatcggc atttgtattt tgtgagttgc
    13441 caggtaatgc ttgatttgaa ccaccttgca cattatgatc cgtagttaaa gcttgtgcac
    13501 catttgggtt tgaaagtaat aaaactgtcc cgattaaagt agagaatatc cctactttat
    13561 attttctaat actatattta tttttcttta acaaattcat ttcgttttcc tcctggttgt
    13621 ctattctctg ctgtgttgtt tagaattatg ctaaaaatat aagctccccg attatgttta
    13681 taaccatatt attttgtaca acattttaag ttcactcaat tttatcatca tcattttata
    13741 aaataaagtt ttattttatt acaatttagt ttccgttttg taattaatca cttaaaaaat
    13801 cataacaaga acgctttaga ttgttttgta acagaaagca attcatagca aaaaacacca
    13861 agcaaccgaa acgttacttg gtgtcattac aaatgtaata ttattttaaa ttatgtcact
    13921 actttaaata ctttttattt ccaaaccttc atcgccccaa gcgtgcatgc cgccttcgac
    13981 atttacggca tcaatgccat ttgcctctaa atattctaca actttagcgc ttcgaactcc
    14041 accagcacat acaatatagt atgtctcttt tttgttaaat gcatttaaat tgtcgggaat
    14101 ggtatccatt ggaattaact ttgcattagg tatatatccc attgctgttt cttcgtcagt
    14161 acgaacatca acaatttgaa ctggtttaga ttctaaaagt ttggttttta attcatctgt
    14221 agtaattgac ttcattttaa agtcctcctt ataacattat ttagctacaa tattgactaa
    14281 tttttgagga acagcgatga ctttcatgat gtctttacct tcaatactcg ctttaacatt
    14341 gtcattagat aaggcaattt cttgcatttc ttcttttgat gtatctttag caattttaat
    14401 tttagctctc aatttaccat taacttgaac aacgatttct acttcatcat ctacaagtag
    14461 tgcttcgtca taagttggcc aaggttggta cgtaatagac tcttcatgtc ctaattttga
    14521 ccataattct tcaccgatat gtggtgcaat aggtgctaac attttaacga agccttcaat
    14581 gtaaggttta taaacttcat caactttata acactcatta ataaatacca ttaattgact
    14641 aatagcagta ttaaatccta atgtttcaaa gtcttctgtt acctttttaa cagtttggtt
    14701 ataaacttta tctaaagatt tattatttgt agttactatt tttgaactta atgttccatc
    14761 ttcatttacc attaaacgcc atacacgatc taagaatcga cgagacccat ctaatccttt
    14821 ttcactccat gcaattgcag catctaaagg tcccataaac atttcgtaaa gacgtaaagt
    14881 atctgcccca tgagattgta ctatatcatc aggattgatt acatttcctt tagatttact
    14941 catcttctca ttaccttctc ctaaaatcat accttggtta aataattttt ggaaaggttc
    15001 ttttgtaggt acgataccta aatcataaag tactttatgc caaaatcttg catacaataa
    15061 gtgaagcact gcatgttcta cgccaccgat atataaatca acaggtaacc aatgttttaa
    15121 tttttcagga tctgctaaca tattttcatt tttaggatcg atgtaacgta aataatacca
    15181 acaactacct gcccattgtg gcattgtatt tgtttcacga cgccctttca tacctgtttt
    15241 ttcatctaca acatttacaa atgaatcaat attagctagt ggtgactcac cagtccctga
    15301 tggcttgatt tcatctgttt caggtaacaa caatggtagc tcttcttcag gaacagttgt
    15361 cattgttcca tcttcccaat gaatgacagg aattggttcg ccccaataac gctgacgact
    15421 gaataaccaa tctcttaatt tgtaattaac tttcttttcg ccagcacctt ttttctctaa
    15481 taattgaata gctttagtaa ttgccgcttc attttctaaa ccatcaagtt caccagaatt
    15541 aatatgttta ccttcaccag tgtatgccgc ttcttcaaca tttccacctt caatgacttc
    15601 aatgattggc aaatcaaact ttttagcaaa ttcataatct ctgtcatcat gcgctggtac
    15661 tgccataatt gcaccagtac catatgttga taatacataa tcagcaatcc aaatttgtac
    15721 tttttcacca gataaaggat taattgcata tgcaccagta aacacacctg atttatcttt
    15781 tgctaaatct gtacgttcta aatcagactt tttagaagct tctgtttgat acgctttcac
    15841 ttgatcttta tattcatcag ttgtaattga atcaaccaat gcatgctcag gacttaagac
    15901 taagaatgat gcaccataga ttgtatctgg tctagtcgta aatacttcta cttttccttc
    15961 cgaattatct acatcaaatg aaactttggc accttctgaa cgtccaatcc agttacgttg
    16021 catatctttt aaagactcag gccaatctaa atcatctaaa tctgctaata attgatctgc
    16081 atattctgta attttaagta cccattgttt cattggttta cgataaactg ggtgaccgcc
    16141 acgttcagat acaccgtcaa tcacttcttc gttagataag acagtaccta atgcaggaca
    16201 ccagttaact gcaacttcgt caacgtatgc taaacctttg ttatataact gtatgaaaat
    16261 ccactgtgtc catttatagt attctggatc tgttgtatta acttcacgat cccaatcata
    16321 actgaaccct aattctttaa tttgtcgttt aaaagtttgg atatttttct ttgtaaattc
    16381 acgtgggtcg ttaccagtat ctaaagcata ttgctctgct ggtaatccga atgcatccca
    16441 ccccatcgga tgtaatacat tatatccttg cattctctta tatcttgaaa tgatatctgt
    16501 tgctgtatag ccttcaggat gtccaacatg taaaccagca cctgatgggt atggaaacat
    16561 gtctaaagca taaaatttct tttgacctaa gttatcattt gttttaaatg ttttattttc
    16621 gtcccaatag tcttgccatt tcttttcaat ttgattgtgg ttgtaattca acacatttcc
    16681 tcctattcaa taaaaaaaga cacctctaac tattctattt acatagggac gacatttgcc
    16741 gcgttaccac cctatttcac aaaaattgtg cactcatttt aatagtaaga gatgtcattc
    16801 attcaattta aaattaatac gatttacatt tagtccttaa cactagctct cagcatcact
    16861 agctttctgt agaaaagctc caaacgaaat taaattatac aaattaatat tatgttataa
    16921 cgacatttat ttcaagtcta taacgttaat tctatttact cattatgcat ctattttttt
    16981 aggttgcgta ttattctcct tgaaaaccat taataatatt aatgcaaata caagtagtac
    17041 catcataccg ataaacatca tgcgcatatt aaatgcatca actaatacac caccaagaaa
    17101 tggaccgaat gcttttccta ctgtagcagc tgaattcaca aaaccttggt actgtccttg
    17161 cttaccatct ggcgctaact gattggctat agttggaact gcaggccata caaacatttc
    17221 tccaaaagtt aaaataatca taccgacaac aaatattgta aagttttcgg caaaactcgt
    17281 gacaaagaac gacaacataa aaatgatgat gccgacaaac atttgcttct ttaagtttcc
    17341 ttttaacaga tagagaattg gtttaattaa tggttgtgct actaaaatca ttattccgtt
    17401 aattgtccat aaaacactat attgtgccat tgaaatatta atcgattgtg taaatgatgc
    17461 gattgtagac tcccattgaa tatacgcaac ccaacaaatt gcaaacattg cacaaattag
    17521 tactaatgaa ataaatcttg ctttattctt tttaccagta atatctaaat gagttggata
    17581 tttaactttc gcattaattt caatattaaa ttgcgttacc gcgacaagtg caaacacaac
    17641 atacataata agattggcta aaaagatata gttaaagcta aattctgcga caaagccgcc
    17701 cattgcagcg ccgacagcaa caccaatatt ttgcgctaag tatatcgcat taaacgtttg
    17761 tcttccgcca ttcggccaca ctgctccagc cattgcgtat atcgcaggaa taatcattcc
    17821 gccaccaaag cctaacatta caagccatac agcataccaa ggccacccgt gaaagaaatt
    17881 aagtagcgtt gtactacaaa gacaagtgaa agttccaatt aaaatcgtct tgtatccacc
    17941 taatttatca aatagtgaac cacctaataa gtttccaata accatgccaa atgaatttat
    18001 cattagcact aaaccagcaa cagttaaact ttttccaagt tcttgtttca tataaattgt
    18061 atttaaaggc cacaaaaaac tggaaccagt aatatttaac gccatgccaa ttactagcca
    18121 ccagactgat ttaggtatat tcatatttca tagttcctct ctcttttttg tcaaactata
    18181 ataaacacta taacattaaa atatgttaaa atctgtactt agaactcttt ttttatagaa
    18241 aggaaaatga cttatgggca atcatttcca atacgctttt gaaaacaaac gttatcacac
    18301 atggaattac catttaaaaa ataaatttgg acaaaaaata tttaaagttg cattggatgg
    18361 cgggtttgac tgtcctaatc gtgatggcac tgtagcacat ggtggatgta cattttgttc
    18421 tgctgcaggt agtggagact ttgcaggtaa tcgtgcagat tcaattgcag tacaatttaa
    18481 agaaattaag gaaaagatgc atgagaaatg gcacgaagga aaatatattg cttattttca
    18541 ggcatttaca aatacacatg caccagttga agtgttaaaa gaaaaattcg aacctgtact
    18601 taaagaaccg ggtgttgtgg gattatctat tggtacgcgt cctgactgtc taccagacga
    18661 tgttgtcgaa tatttagcag atttgaatca acgaacatat ttatgggttg aattgggact
    18721 acaaacaatc catcaatcaa catctgattt aatcaatcgt gcccatgata tgcaaactta
    18781 ttatgatggc gtggcaaaat tacgtaaaca taatatcaat gtatgtacac acatcattaa
    18841 tggcttacct ggcgaagact atgacatgat gatggctact gccaaagaag ttgcacaaat
    18901 ggatgtacaa ggtattaaaa ttcatttact tcatttgtta aaaggtacac cgatggtaaa
    18961 acaatatgat aaaggtttat taacttttat gactcaagaa gaatacacaa acctagttgt
    19021 agaccaatta gaagtgattc cccctgaaat gatcgttcac cgaattaccg gtgatggtcc
    19081 aatagatatc atggtcggtc caatgtggag tgttaataaa tgggaagtat taaatggcat
    19141 cgacgctgaa ttagcacgta gaaattctta tcaaggcttg cgttacaagt ctaaggtgaa
    19201 gcaatgaaat tagaacgaat actccccttt tcaaaaacac ttattaaaca acatataaca
    19261 ccagaaagta ttgttgtaga cgcaacttgc ggtaacggca atgacacttt atttttagcc
    19321 gaacaagtac cagaaggaca tgtttatggt ttcgacattc aagatttagc tttggaaaat
    19381 acacgtgata aagttaagga tttcaatcat gtttctttag taaaagatgg acatgaaaat
    19441 attgaacatc atataaatga tgcacataaa ggtcatattg atgcagccat ttttaaccta
    19501 ggttatttgc ctaaaggtga taaatctatc gtgacaaagc ctgacacgac aatccaagct
    19561 attaatgcgt tattatcttt aatgtcaacc gaaggtatta tcgtattagt tatctatcat
    19621 ggtcatagtg aaggccaact tgaaaagcaa gctttgcttg attatttgag tactttggag
    19681 caaaagcatg cgcaagtttt gaaatatcaa tttttaaacc aacgtaatca tgctccattc
    19741 atttgtgcca tagaaaaaat ttcttaaaca tttgctgcgc tatacttttt tataatttag
    19801 gattgagtag ctaacatttg aaaaaaatat caacgacaaa aagaggtttt caacttaatg
    19861 aaaacctctt tttatgctat taaacttaca cagcaataat tgcgtttaaa ctattttgca
    19921 ttgctgttgc tctacttgca atcgcatcac tgatgaaatt caacaactct actttctctt
    19981 gttgtaactt ttcattgaaa tgaataataa ctgttctttc atcgtcaaca ggacgctctt
    20041 tgtaaatcca ttctaattca actaaattat tatacgttct agtacgctta tacggtttaa
    20101 cttcaacaaa tctgtccatt tctttaagcg tcatagaacc tttttgccat aaagttagta
    20161 aaattaaaat ttcttctcta gacattttgt actcgctttc aatctcgctg aaaattgagt
    20221 taatgtcacc caaaagtgtt tctaattgca aaatcccaaa tacagtatcg ttatttactt
    20281 ttttcatgct atacatctcc caattaataa tcacatattt agctaccata caagtattaa
    20341 caaacatccc aacaatccca aaacttatat gtgctaactt atgcataaaa ctacaagtgt
    20401 aaataaactt gctttctatt caattgtcta agttgaataa aattaaataa gtataaaaac
    20461 caataatcac tagagttaat atttagaaat aaga
//
LOCUS       DAKSQE010000042        20493 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.042, whole genome shotgun sequence.
ACCESSION   DAKSQE010000042 DAKSQE010000000
VERSION     DAKSQE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20493
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.042"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            32..856
                     /locus_tag="O0V33_001286"
     CDS             32..856
                     /locus_tag="O0V33_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCX8975926.1"
                     /translation="MIKNNKTVEDTYSTKAIVDSISSSVQMKQVMVQQTPGRYMLKSM
                     MAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLVLVVLTNSELLTSNFMY
                     FTVGWYYKVVSLNKMMWILLYCFLGNILGGFVLFFLMKYAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVIVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            1324..1860
                     /locus_tag="O0V33_001287"
     CDS             1324..1860
                     /locus_tag="O0V33_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCX8975927.1"
                     /translation="MANTTIRLANENDAVALHQLMHEAFTPLRELGIDWPSVNANLDA
                     VKENIDKNTTFVMTIDDEIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQ
                     LLTYVEETFLRDTLKAAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMR
                     KVLIPERFDETILGQPPF"
     gene            2088..2840
                     /locus_tag="O0V33_001288"
     CDS             2088..2840
                     /locus_tag="O0V33_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HCX8975928.1"
                     /translation="MNGNIIVAHGMRRGRQNQALEAFISELVKDDIHHYHIAFLESEH
                     QDLETVMTTLIQSGVKQLKIVPLLLFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLG
                     THPLMTEIIMQRIDEVRVDEMQQVGVVVVAHGNINGKFTKAHEELQTCVQHLQINKPT
                     YARTLYGEISFIHDLEDIARQYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHITP
                     SINFDPILKQIINDRLESLNDSNENIKALTKS"
     gene            2886..5291
                     /locus_tag="O0V33_001289"
     CDS             2886..5291
                     /locus_tag="O0V33_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCX8975929.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHDIKLITNDPVVDVDRANQNVTTANGIEVAY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTIDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPTDLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARKIFEV
                     EKV"
     gene            5295..5609
                     /gene="nirD"
                     /locus_tag="O0V33_001290"
     CDS             5295..5609
                     /gene="nirD"
                     /locus_tag="O0V33_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCX8975930.1"
                     /translation="METKEKIKVTTLDELTPLIGKKVIVKGKEIGLFLTESGTIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            5600..6577
                     /gene="cobA"
                     /locus_tag="O0V33_001291"
     CDS             5600..6577
                     /gene="cobA"
                     /locus_tag="O0V33_001291"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCX8975931.1"
                     /translation="MSVNGYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYSKSTTEIIDVGKKPYAKHIQQEKINECIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNNFNIAFEIVPGVTSASAAVATMQTGLTMRAVAKSVTFSTGYFKDSEENEVD
                     VNALVNGGTLAIYMGVKRLEKIIAQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNIV
                     GKLEHYAIEAKPGICIIGEVVGYTENVSTTSNPTQQFYVVSGSKPDALMLCEHLYDEG
                     YGCLLNPNDTSNGTYHSSQYDYYDTFIKQQENVTYISTDRADTNTVLCH"
     gene            6884..10573
                     /locus_tag="O0V33_001292"
     CDS             6884..10573
                     /locus_tag="O0V33_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCX8975932.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTDIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFIIMLDEDENGYKAGRFLRASDLGQTTEQSEWKPVIHDTISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMTEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESNGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEE
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIDSNEAILQRAIDEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILNISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNVERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            10563..12122
                     /gene="narH"
                     /locus_tag="O0V33_001293"
     CDS             10563..12122
                     /gene="narH"
                     /locus_tag="O0V33_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCX8975933.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGNHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IKKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGISYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTNQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTKPVKEALQRMAMMRSYMRSQVTQQPFDTSRLERLGLTERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            12115..12690
                     /gene="narJ"
                     /locus_tag="O0V33_001294"
     CDS             12115..12690
                     /gene="narJ"
                     /locus_tag="O0V33_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCX8975934.1"
                     /translation="MINFDNFKKYQESFGYIAQQLCFPEKLTFHPKTFEETISKDHPG
                     YDDLIAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            12683..13360
                     /gene="narI"
                     /locus_tag="O0V33_001295"
     CDS             12683..13360
                     /gene="narI"
                     /locus_tag="O0V33_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCX8975935.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKSWMDAAGVSEHLYHIGAVYIGSIFGIITLV
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNVQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKIHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            13380..13832
                     /gene="nreA"
                     /locus_tag="O0V33_001296"
     CDS             13380..13832
                     /gene="nreA"
                     /locus_tag="O0V33_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HCX8975936.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMIIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRSLPKIFDNDDIQRKFGIFNDDK"
     gene            13856..14890
                     /gene="nreB"
                     /locus_tag="O0V33_001297"
     CDS             13856..14890
                     /gene="nreB"
                     /locus_tag="O0V33_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HCX8975937.1"
                     /translation="MINEDSIQLEALLKKYYEHSIEKIVFADDSGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTAKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGVKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            14913..15566
                     /gene="nreC"
                     /locus_tag="O0V33_001298"
     CDS             14913..15566
                     /gene="nreC"
                     /locus_tag="O0V33_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HCX8975938.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTSDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(15820..16560)
                     /locus_tag="O0V33_001299"
                     /pseudo
     CDS             complement(15820..16560)
                     /locus_tag="O0V33_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(16539..17453)
                     /locus_tag="O0V33_001300"
     CDS             complement(16539..17453)
                     /locus_tag="O0V33_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HCX8975939.1"
                     /translation="MYKINIIRSDSVYKNILKTPLNDRDSIFTKEILVPFKKKFEVQQ
                     IPIHNDAKQTMSAIQFLDAFQKSPKDLRMSDQMSIQYLNNDFWSNCEKYLKVAIDQFA
                     NYSISSQVSNYHFTVLLGDSQKPLMYLNKNRGGDGGIPGYIMIYLVPSTSTINSMKSL
                     IAHEVNHNMRYQYIDWDGGSLIELIIAEGLAENYVESLYGKAHIGPWVTNTNWSRDNV
                     KIKNTIYNHLHLKHIFESMPFLYGDDINKLQGRSIVGLSHAAGYACGYHLVKYFLQKT
                     NIPIEVATTLPAQKIINEVNDFWHTHTL"
     gene            17962..19131
                     /locus_tag="O0V33_001301"
     CDS             17962..19131
                     /locus_tag="O0V33_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HCX8975940.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLGQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VIMIIGAVILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFSNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKHS"
     gene            complement(19239..19667)
                     /locus_tag="O0V33_001302"
     CDS             complement(19239..19667)
                     /locus_tag="O0V33_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HCX8975941.1"
                     /translation="MILNFNQFENQNFFNGNPSDTFKDLGKQVFNYFTTPSFVTNIYE
                     TDELYYLEAELAGANKEDISIDFNNNVLTIQATRSAKYKSGKLILDERNFESLIRQFD
                     FEAVDKQNITASFENGLLTITLPKIKPSNDTTSSTSIPIS"
     gene            complement(19884..20330)
                     /locus_tag="O0V33_001303"
     CDS             complement(19884..20330)
                     /locus_tag="O0V33_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCX8975942.1"
                     /translation="MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKDYDLTYTGYIVL
                     MAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQG
                     KAIKSPLAEISVKVFNEFNISEREASDIVNNLRNFVSKNFDNSDKK"
BASE COUNT         6940 a         3023 c         4102 g         6428 t
ORIGIN      
        1 ttatttgata atagtaaatg ggggaagtgc tgtgataaaa aataataaaa cagttgaaga
       61 tacgtattca actaaagcaa tcgtagactc aatatcatca tctgttcaaa tgaaacaagt
      121 gatggtacag caaacaccag gacgatacat gcttaaatct atgatggcag gtttcttatt
      181 gtccattgtt actgttttta tgtttggtat taaaacacaa ttcgcaagta cacatgttga
      241 tggtctcatt aatttaatgg gagcaattgc tttcagctta ggattggtct tggttgtact
      301 aacgaattct gaattattaa caagtaattt tatgtatttt acagttggct ggtattataa
      361 agttgtttct cttaataaga tgatgtggat tttattatat tgtttccttg gaaatatttt
      421 aggtggtttt gtattattct tcttaatgaa atatgcacat gttatgacac cagaaatgac
      481 acaagcctta actgccttag ttcagaaaaa gacagtcgat tcgacttggt tgaatatttt
      541 tacaaaaggt attttctgta acttctttat taatatcggt atttttattt ctatgcaatt
      601 caaaggcgga ttgacaaaag cattcttcat tgcttgtggt gttatagtct ttgtatacat
      661 gggatatgag cacgttgtat tcaatgctgg tttatatgca ggtatggtgt tcttcaacct
      721 tgatgctgta tcttggttac atgtattaaa aaatatcgtt tttgcattcc ttggtaactt
      781 tgtaggtggc ggtatttttg taggacttgt ttatgcattt ttaaatggta aacgcaatcg
      841 tttagagcaa cagtaatgaa gtaataattg aagatgttgt gacaacaatg aatttagaat
      901 catccttttt gggggcatcg ttccccaact tgcacattaa tgtaagctgg cctgccgtca
      961 gcttctgtgt tggggccccc gccaacttgc attgtctgta gaatttcttt ttgaaattct
     1021 ctatgttggg gccacgcagt cagctactgc caatataaca ttgtagagtc taggacatta
     1081 tgaactgcac gcttcaaagt agacattgaa aaatgaaagc tttgaagcgt gcatatcatt
     1141 aattcatgtc ctagactttt actttgcttt caataatgaa ataattgatg atcacatact
     1201 tatcttatat caatgaggca tgttatctga cacatgtgct aaatgttggt acgctataaa
     1261 agataagcat agctaaaatt aaagatgatt catttaaaat aaacagaaag tgtgtggatt
     1321 caaatggcaa atacgacgat tcgactcgca aatgaaaatg atgcagtagc actacatcaa
     1381 ttaatgcatg aggcgtttac accgttgaga gaacttggca tagactggcc ttcggtaaat
     1441 gcaaatttag atgctgtgaa agaaaatata gataaaaata ctacatttgt catgacaatt
     1501 gatgatgaaa ttatatctac gattacggtt agatatcctt ggggaagtgt gaaaagcatt
     1561 tcgggctatc cttttgtctg gtggtttgcg acaaatcctg agtatgaagg gaaaggctat
     1621 ggtagtcaat tacttactta tgttgaagaa acatttttac gagatacatt aaaggcagct
     1681 gctgtaactt taggtacatc tgcaagattg catccgtggt tacttaaaat ttatgaaaaa
     1741 agaggttatg aaatatacag cgaacatgaa aatgatgatg gcgatttagg tgtgatcatg
     1801 cgcaaagtat tgatacccga gcgctttgac gaaacaatat taggccaacc accattttaa
     1861 gataataata tacactacaa gcgaccgata tattgatgct tgctcaaata aatgttgagt
     1921 agggattcat atatggtcgc ttttttgatt taacaaaaag ggaaaattta taaggaatat
     1981 gcatgactac aggcatcagg gaattccctg attttacatt ataatgtgat tcttttcaca
     2041 aaaacgaaat gatttggtat acaatacgtg tgaaagggtt ggtaaatgtg aatgggaata
     2101 tcattgttgc acatggcatg aggcgcggtc ggcagaatca agcgttagaa gcgtttatat
     2161 ctgaattagt aaaagatgac atacatcatt accatattgc atttttagaa agtgagcatc
     2221 aggatttaga aacagtaatg acaacgttga ttcaaagtgg cgttaaacag cttaagattg
     2281 tacctttgct tctctttagt gccatgcact atctcaagga tataccgaat atcgttcatg
     2341 aaatgaaacg acggtatcca gacataaagg tagaagtaag cgagccactt ggcacacatc
     2401 ctttaatgac agaaataatc atgcaacgta ttgatgaggt gcgcgtggat gaaatgcaac
     2461 aagtcggtgt tgtcgttgtt gcgcatggca acattaatgg caagtttaca aaagcacatg
     2521 aagagttaca gacatgcgtt caacatttgc agattaataa acctacttat gcgagaacgc
     2581 tatatggtga gattagtttt atacatgatt tagaagatat tgcgagacag tatcaaaaat
     2641 taattgtcgt acctttattt ttatatgacg gaagacttgt taacaaagtg aagcagcaaa
     2701 tgaatgacat ggttattaat acagatatac acatcacacc ttcaattaac ttcgacccga
     2761 ttttaaaaca aattattaac gatagattag aaagtcttaa tgactccaat gaaaacataa
     2821 aagcactaac gaaaagttaa cacctttttt tgaaataaat attatgtagt aaaggggcat
     2881 attttatggc aaagcaaaaa ctagtaatga ttggtaacgg tatggcgggc attcgaacaa
     2941 tcgaagaaat attagagcgt gccaatgatt tatatgatat tacggtaatt ggtaaagaac
     3001 cttatccaaa ctataaccgc atcatgcttt caaatatttt acaaaataaa atgacagttg
     3061 aagaaacaat tatgaatcca tatgagtggt atgaagaaca cgatattaag ttaattacca
     3121 atgatccagt agttgacgtg gatagagcaa atcaaaatgt aacgactgca aatggtattg
     3181 aagtagcata cgataaatta atatttgcga caggatctaa agcatttgtc attccagtgc
     3241 caggttcgac attaccaagt gttattggct ggagaacaat tgatgatact gaacaaatga
     3301 tgaacattgc taagactaaa aagaaagcaa ttgtaattgg tggaggctta ctaggtttag
     3361 agtgtgcacg tggtttatta gatcaaggta tggaagtaac ggtgttacat ttagctgaat
     3421 ggttgatgga aatgcagcta gaccgtaaag ctggaaatat gcttaaagca gatctagaaa
     3481 agcaaggtat gaagtttgaa atgcaagcca acacaactga aatcttagga gaagatgatg
     3541 ttgaaggtgt taaattggct gatggacgcg agattccgac agacttagtt gttatggcag
     3601 taggtattcg accatacaca gaagtagcca aagaatcagg tctagatgtt aatcgtggta
     3661 ttgttgtcaa tgatgtgatg caaacaagtg atagtaatgt atatgcagtt ggtgaatgtg
     3721 cagaacataa cggcaaagtt tatggactcg ttgcaccact atatgaacaa ggtaaagtat
     3781 tagctgatca tttaacaaat aaagaaacga acggatacaa gggatcaaca acatttacgt
     3841 cattaaaagt ttctgggtgt gacctatata gtgctggtca aattgttgaa aatgctgaaa
     3901 ttaaaggtat tgaaatattt aatagcgttg ataataacta taaaaaaatc tttttaaaag
     3961 acggtaatgt agttggtgca gtactatatg gtgatatcga tgatggttca cgcttttata
     4021 acatgatgaa aaaaggtgaa tctactgaag actacacact ggtatcattg cttactaaag
     4081 gtggagaaga ggcatcgcta tcaattgctg atatggctga tgatgaaaca atttgtggct
     4141 gtaatggtgt cgataaaggc acgatagtaa atgcaattac ggaaaatggt tttactacag
     4201 ttgaagaagt tacggctaaa acgaaagcag gtaattcatg tggtaaatgt aaaccgcaaa
     4261 ttgctcaaat cttacagcac acgttgggcg atgactttgt tgctgctaaa cctgcaggta
     4321 tatgtggttg tactgatttg acacgtgatc aaattgttac gcaaatacga gcgaaaggtt
     4381 taaaaacatc taaagaagtt cgacatgttt taaactttaa aaacaaaggt ggatgtccaa
     4441 aatgtcgacc tgcgattaac tattatttga atatggttta tccacatgat catgaagatg
     4501 aaagagaatc aagatttgct aacgaacgtt accatgcgaa tattcaaaat gatggtacat
     4561 tttctgttat acctcaaatg cgtgggggtg ttacagatgc agaccaactg attcgtctag
     4621 gagaagtggc taagaaatat catgtgccac tagttaaagt gacaggttcg caacgtgttg
     4681 gtttgtatgg agttaaaaaa gaagaattac caaatatatg ggaagaccta ggtatgcgtt
     4741 cagcatcagc ttatggtaag aaaacacgct cagttaaaag ctgtgttggt aaagagtttt
     4801 gtcgatttgg tacgcaatac acgacacgac ttggcattcg tttagaaaaa acatttgaat
     4861 acatcgatac acctcataaa ttcaaaatgg gtgtatcagg ctgcccaaga agttgtgttg
     4921 aatcaggtgt taaagatttt ggtattatct cagttgaaaa tggattccaa atctatatcg
     4981 gtggtaatgg tggtacagaa gttgaaaaag ctgaattttt aacaactgta gaaacggaag
     5041 atgaagtaat caaattatgt ggtgctttga tgcaatatta tcgcgagaca ggtatttatg
     5101 ctgaaagaac agcaccgtgg ttaagaagac tagggtttga aaatgttaaa gaagtgttgc
     5161 ttgatccaga aagacagaat gaattatttg aacgtattat ggatgctaaa aaagcagtcg
     5221 aagctgaacc ttgggaagca atcactagta atgcgcaagc tcgaaaaatc tttgaagtgg
     5281 agaaggtgta atacatggaa acgaaagaga aaatcaaagt gacaacttta gatgaattga
     5341 cacccttaat tggtaaaaag gttattgtca aaggcaaaga aataggattg tttttaactg
     5401 aaagtggtac aattcacgcg attcacaata tttgtccgca taaacaaggg ccattgtcag
     5461 agggaacagt gagtggtgaa tatgtattct gtccactcca tgatcaaaag attgatttaa
     5521 atactgggat tgtacaagaa cctgatgaag gttgtgtaga tgtatatgag gtagaagtta
     5581 cagacgggaa cgtatatata tgtctgtaaa tggttacggc aaggtttact tgataggtgc
     5641 gggcccaggt aatccgaatt atttgactaa aaaggctgag cgtttaatac gcgaagcgga
     5701 tgttatacta tatgatcgat tagtgaatcc gttgatttta caatattcga agtcgacaac
     5761 tgagattata gatgttggta aaaagccata tgcaaaacac attcagcaag aaaaaataaa
     5821 tgaatgtatc gtggaagcag cacgtcgata taacaaggtt gtgaggctta aaggtggcga
     5881 cccagcaata tttggacgag ttcaagaaga agttgacaca ctaaacaact ttaatattgc
     5941 gtttgaaatt gtacctggtg tgacatctgc gagtgctgca gttgctacga tgcaaacagg
     6001 attaacgatg cgtgctgttg ctaaaagtgt tacattttcg acggggtatt ttaaagattc
     6061 agaagaaaat gaagtagatg tcaacgcttt agtaaatggt ggtacgttgg cgatatatat
     6121 gggagttaaa cgtttagaga aaattatcgc ccagatacaa cagtacacag atattgatta
     6181 tccgatagcg attgtttttc aagcatcttg ttttaatgaa ttcgtcgtta aagggcgctt
     6241 aagtaatatt gttgggaaac tggagcatta cgcaattgag gcaaaaccag gtatatgtat
     6301 tataggcgaa gtagtgggtt atacagaaaa tgtctcaaca acttctaatc cgactcagca
     6361 attttatgta gtaagtggtt ctaaacctga cgctcttatg ctctgtgaac atttatatga
     6421 cgaaggttat ggctgtttgc taaacccaaa tgatacatcg aatggtacat atcattcgtc
     6481 gcaatatgat tactatgata cgtttattaa gcagcaagaa aatgtaacat atatttcaac
     6541 cgatcgtgca gatactaata cagtgttatg tcactaattt ataaaaataa atgaataagt
     6601 aaggtttcaa ccgagagaat atattcgtgt tgaagcctta tttgtcgttg tgccaaattt
     6661 gaacgattta gattggcaat aagcatgacc acacatgatt gtgtattatt tcaatgaaat
     6721 cccgttattg atagggaaat tccttaagta attaggtaat tccctaatat tttatatttg
     6781 tgaaaaaatg tacaatctaa ttaaagcaat agtcttgggc atttttagta tttaaaaaca
     6841 cgacatctac acatcgtatt tttatacgta ggaggatata aatatgggaa aatttggatt
     6901 gaatttcttt aagccaacag aaaaatttaa tgggaattgg tcgatcctag aaagtaaaag
     6961 tagagaatgg gaaaaaatgt acagagaacg ttggagtcat gataaagaag taagaacaac
     7021 acatggtgtt aactgtactg gatcatgttc ttggaaagtc tttgtgaaaa atggtgtgat
     7081 tacctgggaa aatcaacaaa ctgactatcc aagttgtggt ccggatatgc ctgaatatga
     7141 accgagaggc tgtcctcgtg gtgcatcatt ctcttggtat gaatacagcc cacttcgaat
     7201 taaatatcca tatattcgtg ggaaactttg ggatttatgg actgaagcat tagaagaaaa
     7261 caatggcaat cgtgtggctg catgggcttc tattgttgaa aatgaagaca aagccaaaca
     7321 gtataagcaa gcccgtggta tgggtggaca tgtgcgttca aattggaaag atgttacgga
     7381 tattatcgca gcacaattac tatatacaat taaaaaatat ggtcctgaca gaattgccgg
     7441 attcacaccg attccagcga tgtctatgat tagctatgca gcaggtgctc gattcatcaa
     7501 tttacttggt ggtgaaatgc ttagttttta tgactggtat gcagatttac caccggcctc
     7561 tccacaaatt tggggagaac aaacagacgt acctgaatca agtgactggt ataacgcatc
     7621 atacattatt atgtgggggt ctaatgtacc actaacgcgt acgccagacg cacattttat
     7681 gacagaagtt cgctacaaag gaacaaaagt tatttcagta gcacctgatt atgcagaaaa
     7741 tgtgaagttt gctgataact ggttagcacc gaatcctggt tcagatgctg caattgctca
     7801 agcaatgaca catgttattt tacaagagca ttatgttaat caaccgaatg aacgctttat
     7861 taattatgcg aaacaatata cagacatgcc atttatcatc atgttagatg aagatgaaaa
     7921 tggatataaa gcaggtcgat ttttaagagc aagtgactta ggacaaacga cagagcaaag
     7981 tgaatggaaa ccggtaatcc acgacacaat cagtgacagt ttagtggtac ctaatggcac
     8041 aatgggtcaa cgttgggaag aaggtaagaa atggaactta aaactagaaa cagaagatgg
     8101 ttctaaaatt aaccctacat tatcaatgac agaaggtggg tatgaattag aaacaattca
     8161 attcccatac tttgatagtg atggagatgg gatattcaat cgcccaattc caactcgaca
     8221 agtcacttta gcaaatggtg acaaagtccg tattgctaca atttttgact taatggcaag
     8281 tcaatatggc gtgcgtcgtt ttgatcataa attggaatca aatggatacg acgatgcaga
     8341 atcaaaatat acacctgctt ggcaagaagc catttcaggc gtaaaacaaa gtgttgtcat
     8401 tcaagtagct aaagaatttg cgcaaaacgc tatcgatact gaagggcgtt caatgattat
     8461 catgggtgcg ggtattaacc attggtttaa ctcagatacg atttatcgtt caatcttaaa
     8521 cttagttatg ttatgtggct gtcaaggtgt gaatggtggc ggttgggctc actatgtggg
     8581 acaagaaaaa tgtcgcccga ttgaaggatg gagtactgtc gcatttgcga aagactggca
     8641 aggaccacca cgtttgcaaa acggaacaag ttggttctat tttgcgacag accaatggaa
     8701 atatgaagag tcaaatgtag atcgtttaaa atctccatta gctaaaacag aggagttaaa
     8761 gcatcaacat ccagctgatt ataatgtgtt agcagctaga cttggttggt taccatcata
     8821 tccacaattt aataaaaata gcttgttgtt tgcagaagaa gctaaagatg aaggtataga
     8881 ttctaatgaa gcgattttgc aacgtgcaat tgatgaagtg aaatcaaaac aaacacaatt
     8941 tgcaattgaa gatcctgatt tgaaaaagaa tcatccaaaa tcattattta tatggcgttc
     9001 aaacttaatt tcaagttctg caaaaggtca agaatacttt atgaagcatt tacttggcac
     9061 aaaatcagga ttattagcta caccaaatga agatgaaaag ccagaagaaa ttacgtggcg
     9121 tgaagaaaca acaggtaagt tagatttagt ggtctcttta gacttcagaa tgacggcaac
     9181 accgttatat tctgacattg ttttgccagc agcgacttgg tatgaaaaac atgatttatc
     9241 atcaacggat atgcatccat atgtacatcc tttcaatcct gctattgatc cattatggga
     9301 atcacgttca gactgggata tttataaaac attggcaaaa gcattttcag aaatggcaaa
     9361 agactattta cctggaacgt ttaaagatgt tgtgacaaca ccacttagtc atgatacaaa
     9421 acaagaaata tctacaccat atggcgtagt gaaagattgg tcgaagggtg aaattgaagc
     9481 ggtgcctgga cgtacaatgc ctaactttgc aattgtagaa cgcgactaca ctaaaattta
     9541 cgacaaatat gtcacacttg gtcctgtact tgaaaaaggg aaagttggag cacatggtgt
     9601 aagtttcggt gtcagtgagc agtacgaaga actaaaaagt atgttaggca cgtggagtga
     9661 tacaaatgat gattctgtaa gagcgaatcg tccacgtatt gatacagcac gtaatgtcgc
     9721 tgatgccata ctaaatattt catcagctac gaatggaaag ttatcacaga aatcatatga
     9781 ggatcttgaa gaacaaactg gtatgccgtt gaaagatatt tctagtgagc gtgctgctga
     9841 aaaaatttcg ttcttgaata taacatcaca accacgtgaa gttataccaa cagcagtatt
     9901 cccgggatct aataaacaag gccgacgtta ctcaccattt acaacgaatg tagaacgttt
     9961 agtacctttt agaacgctaa caggtcgtca aagttattat gttgatcatg aagttttcca
    10021 acagtttggt gaaagcttac cagtatataa accgacattg ccgccaatgg tatttggtaa
    10081 tagagataag aaaattaaag gtggtacaga tgctttggta ctgcgttatt taacgcctca
    10141 tggaaaatgg aatatacact caatgtatca agataataag catatgttga cactatttag
    10201 aggtggtcca acggtttgga tatcaaatga agatgctgaa aaacacgata tccaagataa
    10261 tgattggcta gaagtgtata accgtaatgg tgttgtaacg gcaagagcag ttatttcgca
    10321 tcgtatgcct aaaggtacaa tgtttatgta tcatgcacaa gataaacata ttcaaacgcc
    10381 tgggtcagaa attacagata cacgtggtgg ttcacacaac gcgccgacta gaatccattt
    10441 gaaaccaaca caactagtcg gaggatacgc acaaattagt tatcacttta attattatgg
    10501 accaattggg aaccaaaggg atttatatgt agcagttaga aagatgaagg aggttaattg
    10561 gcttgaagat taaagcgcaa gttgcgatgg tattaaattt agataaatgc ataggatgcc
    10621 atacgtgtag tgtgacatgt aaaaacactt ggacaaatcg tccaggtgct gagtacatgt
    10681 ggttcaacaa cgtagagact aagccgggcg tagggtatcc gaaacgttgg gaagatcaag
    10741 aacattacaa aggtggttgg gtactaaatc gtaaagggaa acttgaatta aaatctggaa
    10801 gtagaatttc aaaaattgca ttagggaaaa ttttctataa tccagatatg ccactaatta
    10861 aagattatta cgagccatgg aactataatt atgaacattt aacaactgcg aaatcaggta
    10921 atcattctcc agttgctaga gcatattcag aaattacagg ggataatatt gaaattgaat
    10981 ggggacctaa ctgggaagat gacttggctg gtggtcacgt tacaggtcca aaagatccta
    11041 acatcaaaaa aatagaagaa gatattaaat tccaatttga cgaaactttt atgatgtatt
    11101 taccacgttt atgtgaacat tgcttgaatc caagttgcgt tgcctcatgc ccttcaggtg
    11161 ctatgtataa acgtgatgaa gatggcattg tattagttga ccaagacgca tgtcgtggat
    11221 ggcgttactg tatgacaggt tgtccatata agaaagttta cttcaattgg aaaacgaaca
    11281 aagctgaaaa gtgtacattc tgtttcccga gaatcgaggc gggtatgcct acggtatgct
    11341 ctgaaacttg tacaggacgt atgcgatact taggtgtttt attatatgat gcagatcgag
    11401 ttcatgaagc ggcttcagca gtagacgaga aagatttata cgaaaaacaa ttagatatat
    11461 ttttaaatcc attcgacgaa gaagtcattg cacaagctga aaaagatggc attagttatg
    11521 attggattga agctgcacaa aactcaccaa tttataagtt agcgattgaa tataaactag
    11581 catttccatt acatcctgaa tttagaacga tgccgatggt ttggtattgc ccaccactta
    11641 gcccaattat gagctatttc gaaggtaaaa atacaaatca aaatccggat gcgattttcc
    11701 cagccattga agaaatgcgc ttgccgattg aatatttagc gaatattttc acggcaggag
    11761 atacaaaacc agtaaaagaa gcattgcagc gtatggcaat gatgagaagt tacatgcgtt
    11821 cccaagttac acaacaacca tttgatacat cacgtttaga gcgattaggt ttaactgaac
    11881 gccaaactaa agatatgtat cgcttattag gacttgcaaa atatgaggat cgattcgtta
    11941 ttccaacgtc acataaggaa acatatttag atacgtatca tgctcaaggt agtacgggat
    12001 ataactatgg tggcgaacac tttggtgata actgtgaagg ttgtggtgtt gcagtaggtt
    12061 caggaaaaac tggtcaagaa atttataatg agaatttcta tggagggatt ttccgtgatt
    12121 aatttcgata attttaaaaa gtatcaagaa agttttggat atatagcgca acagctatgc
    12181 tttccggaaa aattaacatt tcatccaaag acttttgaag aaacaatttc gaaggatcat
    12241 ccagggtatg acgatttaat agcatttaga aatgtaatga tgacattttc attatcagaa
    12301 atcaaagcga tttatactga tacatttgat tttacaaaaa aagcgccatt atatatgacg
    12361 tataacaagt ttgatacaca aaaagaacgt gggcaaatgt tagcgaaact aaaggtattg
    12421 tacgagatgt ttggactaga aatggtagac aatgaacttt ctgattatct accgttgatg
    12481 ttgcaatttt tacaagtggc agaatggcga aatgatccaa gagctgaaga gaatattcaa
    12541 ttaatcatta tgattattga ggatggaaca tatgtgatgg cgaatgcact tggtgaagat
    12601 aacaatccat atgcctatgt gattcaagca cttagaaaaa cattgaaatt atgtttgaca
    12661 tcaccaaaag gagtgaaaca tcatgcttaa tcagttttta tgggttatat ttccgtattt
    12721 atgtttagtt gtctttgtcg caggtcatat tgcacgttat cgttatgata aattttcgtg
    12781 gacagcaaaa tctagtgaat taattgaaag aaaaagattg atgtggggta gtttgctatt
    12841 tcacttaggt atcattccgg tattttttgg acatgttgta ggattattaa tacctaagtc
    12901 gtggatggat gctgccggtg tgtctgagca tttatatcat attggtgctg tttatatagg
    12961 gagtatcttt ggtataatta cattggtagg gatgttttta ttaacagcga gacgtgttac
    13021 tacgaaaagt attcgcagat taagttcagc gtccgatatc tttgttaatt tcttattatt
    13081 acttattgta tttatgggat gttatgcaac attagtaaca aacgtacagc atccagattt
    13141 taactataga acttcattat caatttggtt tagacaatta tttatgttga aaccagatgc
    13201 atcattaatg agtggtgtac caattgcatt taaaattcat atattattag gtttcactat
    13261 tatggcttgt tggccattca cacgtttagt acatgtttgg agtgtacctt tgacatatat
    13321 aaatcgcaga tatattattt accgtaagaa taaagtgtaa gtataagagg tgaaaggatt
    13381 tgacaccaga agcaattatt gaagatcgta gatttcaaga gacattagat aaaatacgca
    13441 aagaagaagg ttatgatttt gcggcaatcg ctttctatga atctaacaaa ccatcatctc
    13501 caataaaatg gcactatgtt tcaggtaata aaaataatcg gtttaaatta attattttaa
    13561 gaaaaggccg tggattagct ggaactgtta tgaaaacagg gaagcgtatg attatagcta
    13621 atgtcggttt agcattggga ccagaagaaa agattgacta tccaatttta ttaagcgaat
    13681 cattaacagc agtcttagct gtaccgcttt ggtacaaaaa tcaagtgtat ggtgtactat
    13741 tatttggtca aagagatgga cgttctttac caaaaatatt tgataacgat gatattcaac
    13801 gcaaatttgg aattttcaat gatgataaat aatgagatga attagggtgt aggtcatgat
    13861 taatgaggac agtatacaat tagaagcttt attaaaaaaa tactatgaac attcaatcga
    13921 aaaaatagta tttgccgacg atagtggtaa aattattgcg atgaatgatg cagctaaaga
    13981 tatattatcc gaagaagata attatagtgc agtagcgaat gcgatttgtc acagatgtga
    14041 agggtatacg aatgcctatg acgtacaatc atgtaaagat tgtttcttag agtccatgca
    14101 agtacaagct acgaattttc aagtgtttat gaagacgaaa gatcaaaaag tgatgccgtt
    14161 tactgcaact tatcagctta ttgatcagga tagagggatt catgcattta cgcttcaaaa
    14221 tgtctcatca caaattgaac aacaagaaaa attgcatcaa caacgtatga tgcgtaaaac
    14281 gatttccgca caagaaaatg agcgaaaacg aatttctagg gaattacatg atagtgtgat
    14341 tcaagaaatg ttaaatgtag atgttcaatt gcgattgttg aaatatcaag aagatacagc
    14401 aaagcttttg gaagacgcag aaaacattga atacattgtc gctaaattaa tcgatgatat
    14461 acgtaatatg tcggtggaat taagaccagc ctctttggat gatttaggcc ttgaggcagc
    14521 gtttaaatca tattttaaac aatttgaaga aaattatggg gttaaaatca tatatacttc
    14581 caatattaaa aatacccgtt ttgatagtga tattgaaacg gttgtttacc gagtagtaca
    14641 agaagctatt ttaaatgcgt tgaaatacgc tgatgtaaat gaaataaatg taggtatccg
    14701 tcaaactggg cgccatttgg ttgctgaagt aatagacgcg ggaaatggtt ttgatccaag
    14761 ttctaagcct aaaggttctg gactcggttt atatggaatg aatgaacgag ctgaattagt
    14821 cagtggtagc gtcaacatcg aaacgaaaat tggtgagggc accaatgtta cactgaacat
    14881 tccaatttga aacatacatt gggggaataa aattgaaaat agtcattgcc gatgatcacg
    14941 ctgttgtccg cacgggattc tctatgattt taaattatca aaatgatatg gaagttgttg
    15001 caacagctgc agatggtgtc gaagcttatc aaaaagtaat ggaatataaa cctgatgtgt
    15061 tactaatgga tttaagcatg ccaccaggtg agtcaggtct tatcgctacg agtaaaattg
    15121 ctgacagttt tccagaaact aaaattttaa tattaaccat gtttgatgat gaagagtact
    15181 tgttccatgt gttgcgtaat ggtgcgaaag gttacatatt gaaaaatgca cctgatgaac
    15241 aattattgtt agctattcga actgtatata aaggtgaaac atatgtagat atgaaactta
    15301 caacatcttt agtgaatgaa tttgtatcta attcaaatca ggacactgca aacacatcag
    15361 atccttttaa aatcttatca aaacgagaac tagaaatatt gccacttatt gccaaaggtt
    15421 acgggaataa agaaattgca gagaagttat ttgtttctgt aaagacagta gaggcacata
    15481 aaacgcatat tatgacaaag cttggcttga aaagtaaacc agagctcgtt gaatatgcat
    15541 tgaaaaagaa attattagag ttttagagtt tgaaattaat accatacaaa gcgccagagg
    15601 aaatgattat cttctggcgc ttttgtatgt ttcgaaatat atttttttat aaccgtatgg
    15661 aagctaatac tttctccttt tggaaagcca tacttatttt attggaaacg gaataacttt
    15721 taatatagtt attgatatag agaataaatg agtagttgtc tattttggac atgacatgtt
    15781 agttagggtg tattcagtgt tttagtctct aagtgaggat taaaaattgt ctttcttatt
    15841 ctcattgctc aaaaaataat taacagcttc tgaaatatat gatgctaaat tttgattatc
    15901 atatgaatta aagtaatctt tgaaacgcgt atcattttca tatgttttag caatacagca
    15961 taatgtttca tcatcaaaat cagcgattgt gttcataaaa gatttccatt cttccacgat
    16021 atcattacag tcttggattg gataatgact taaagacatt tgattgaatt gattgaagat
    16081 cattgtcaat ttctgtctga tatcctgtga ttgtaagttg tcttgacggt taataaatga
    16141 ttgatatgcc ttcgtatgac catactttat agatgcttct ttatcatatt gtgtctgtag
    16201 tgacttgttt aaaatgctgg tatctattag agagtgtcct ttaataaatt cacatacaga
    16261 gcgtctaatt aattataagt cagaaatctt tttatctaac ttgcttactt gctctgataa
    16321 taactttcgc aattgttcat tatcatatga aatgtattgt tttattttag cgatatcaaa
    16381 atcaaaagac ttgagtatta aaattttttg taatttttct aagtcttgct gtttataaac
    16441 gcgataattt ttatcatttt tatttggaac taataatcct atttcatcgt aataatgtaa
    16501 agttcgttta gttacacctg taatttcaat aatatctttt aaagtgtatg tgtatgccaa
    16561 aaatcattca cctcatttat tattttttgt gctggaagtg ttgtggcaac ttcaataggt
    16621 atgtttgttt tttgtaaaaa gtatttcacc aagtgatagc cacatgcata cccggcagca
    16681 tgagataagc caacgataga cctaccttga agtttattaa tatcatcacc atagagaaaa
    16741 ggcatcgatt caaatatatg ctttaaatgt aaatgattat aaatagtatt tttaattttt
    16801 acgttatcac gactccaatt agtatttgtt acccacggtc caatatgcgc tttaccatac
    16861 aatgattcta catagttttc agctaatcct tctgcgataa tcaattcaat caaacttccg
    16921 ccatcccaat caatatattg atagcgcata ttatgattta cttcatgtgc aattaagctt
    16981 ttcatagaat taattgtact tgtactcggc actaaataaa tcataatata acctggtatg
    17041 ccaccatcac caccgcgatt tttatttaga tacataagtg gtttttggct atctccaagt
    17101 aatactgtga aatgatagtt agaaacttga gatgagatac tataatttgc aaattgatct
    17161 atagcaactt ttaaatattt ttcgcaatta ctccagaaat cattattcag gtattgtatt
    17221 gacatttggt cagacatgcg taaatctttg ggagactttt gaaaggcatc taaaaattga
    17281 attgcactca ttgtctgctt ggcatcatta tgaataggaa tctgttgcac ctcaaatttc
    17341 tttttaaaag gtactaatat ttctttagtg aaaattgaat cacggtcgtt taatggtgtc
    17401 ttgagtatat tcttatagac ggaatctgat ctaataatat tgattttata catgataaac
    17461 ctccttacgt tgtcagcata aagggtaacg taacgtgatt ttcaagcagt aattgtaact
    17521 aattgaaaaa aatttaagaa atgcatgtga gtgatcctta ataatctgat taaaatgatg
    17581 acaaaatagt gtctaaatgc atcgtaaagg gacattgtat aggggaaatt acaccataaa
    17641 taagggaaaa tccttatgtt cactttttca caatcataat aaaataaatg tgtagtcaat
    17701 actttgtcta tattggggag tgggacagaa atgatatttt cgaaaaattt atttcgtcgt
    17761 cccgccccgg caaggttgac tagaattgaa aaaagcttgt tacaagcgta ttttcgttca
    17821 gtcaactact gccaatataa cttcgtagag catggaacat tgatttattt cccaggctct
    17881 ttaaataaca ttgacctcta acatataggc agagtattga tatctattaa aaaataaatg
    17941 attttggtga aggtgaaacg tatgtacaaa acaaaaggtg gctttcaact tacattacaa
    18001 acattaagtt tagtggttgg atttatggct tggagtatta ttgctccttt aatgcctttt
    18061 attaaacagg atgtcaatgt aactgaaggt caaatatcaa tcattttagc gataccagtt
    18121 attttgggat cagtgctccg tgtgccattt ggttatttaa caaatattgt tggtgctaaa
    18181 tgggtattct ttacaagttt tatcgtattg ttattcccga tatttttctt gggccaagca
    18241 caaacgccgg ggatgttaat ggcatcagga tttttccttg gcgtaggtgg cgcaattttc
    18301 tctgttggtg ttacatcagt tcctaaatat ttccctaaag aaaaagtagg tctagcaaat
    18361 ggtatttatg gtatggggaa tatcggtaca gcagtttctt catttttagc accaccgata
    18421 gcgggtatta ttggttggca aacaacagtt agaagttact taattattat cgctttattt
    18481 gcattaatta tgttcatttt tggtgacaca caagaacgta aaattaaagt gccattaatg
    18541 gcccaaatga aaacattatc taaaaactac aaattatatt acttaagtta ttggtatttc
    18601 attacttttg gtgcttttgt tgcatttggt attttcttac ctaactactt agtaaatcat
    18661 tttggaattg acaaagtaga tgctggtatt cgttcaggtg tattcattgc gctggcaaca
    18721 ttcttgagac caataggtgg tatactaggt gataaattta atgcagttaa agtactgatg
    18781 attgattttg ttattatgat tatcggtgcc gttattttag gtatttcaga ccatatcgca
    18841 ttatttactg taggttgttt aacaataagt atttgtgcag gtattggtaa cggcttaatt
    18901 tttaaattag taccatcata cttctcaaat gaagcaggat cagcaaatgg tatcgtatca
    18961 atgatgggtg gtttaggagg attcttccca ccgttggtca tcacatacgt agctaattta
    19021 acaggttcaa gccatttagc atttattttc ttagcggtat tcggatgcat tgcattattt
    19081 acaatgcgcc atttatatca aaaagaatat ggttcattga aacacagttg atatgtaata
    19141 cgaagcattt ttaaatttaa atgataaagc cttaatacca tgtgtaacgt tagtagcatg
    19201 acattaaggc tttagtaatg tattcatttt aaatttaact aagaaattgg aatagatgtt
    19261 gatgaagtag tgtcattgct tggtttgatt ttaggcaggg taatggttaa tagcccattt
    19321 tcaaaactag cagtaatatt ttgcttatca acagcttcaa aatcaaattg gcgtattaat
    19381 gattcgaagt ttcgctcatc taaaatgagt tttccggatt tatatttagc acttctagta
    19441 gcttgaatag taagcacatt attattgaaa tcgatactaa tgtcttcttt atttgcacct
    19501 gctagctcag cttctaagta atataattcg tctgtttcat atatattcgt tacaaatgaa
    19561 ggtgttgtaa agtaattaaa tacttgttta cctaaatctt taaatgtatc acttggatta
    19621 ccgttaaaaa agttttgatt ctcgaattga ttgaagttca atatcatcac ttccattata
    19681 taaatttgta ttacaaagtc attatatgag tagaataaaa aaagataaag taaaaggggg
    19741 ggataaaaaa gttataaatg gatagatttg tgcctgataa ggaatcaggg caaaagaact
    19801 gagcatcgag atttatacaa gcaataaaga taatgaataa acatagcgat gcatacttct
    19861 gcccatcatc ttatgatagg gagttacttt ttgtcggaat tgtcgaaatt tttcgaaaca
    19921 aaattccgca aattattaac aatgtctgat gcttctcgtt ctgaaatgtt gaattcatta
    19981 aatactttaa cagaaatttc agcaagagga ctttttattg ctttaccttg ttctgttaga
    20041 gaaatttgta ggtttctttc atctttctct tcacgtgttc gaacaacgta atctttcttt
    20101 tctaatttct ttagtaatgg tgtcagtgtt ccagaatcta agaatacacg ttcacctaat
    20161 tttttgatgt taagtttttc atcattttca atcgccatta aaacaatgta accagtatag
    20221 gttaaatcat aatccttaag atagtttgtg tattttttaa ttatttcttt tgaagaaaca
    20281 taaaataaaa aacacaactg tttgctaaga tagctgtttt ctacatacat ccaatcactc
    20341 cttgttaaaa taaacaatat tatgaagtgt aatagttgtc aagtactgat ttcatcgcaa
    20401 ttatcgaatt gtctataatt gaatttaaat gttaaataat gcaaaacata tcaatatctt
    20461 agtattttca tcggtaatga ctgatatgac tga
//
LOCUS       DAKSQE010000043        20311 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.043, whole genome shotgun sequence.
ACCESSION   DAKSQE010000043 DAKSQE010000000
VERSION     DAKSQE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20311
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.043"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            149..517
                     /locus_tag="O0V33_001304"
     CDS             149..517
                     /locus_tag="O0V33_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCX8975943.1"
                     /translation="MKTILKTITYLAFTIIGAYAALFILKTIDSHGITDQFNPLVKED
                     DSYVKTTEVSTRMDDQLRSYSQSAFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKEEVPKKALDKLSR"
     gene            698..1270
                     /locus_tag="O0V33_001305"
     CDS             698..1270
                     /locus_tag="O0V33_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCX8975944.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVDD
                     KNKKVINKKTEKEDTVNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDQ"
     gene            complement(1408..1671)
                     /locus_tag="O0V33_001306"
     CDS             complement(1408..1671)
                     /locus_tag="O0V33_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCX8975945.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            1965..2219
                     /locus_tag="O0V33_001307"
     CDS             1965..2219
                     /locus_tag="O0V33_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCX8975946.1"
                     /translation="MFGFIGMLIVGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWVG
                     GKLFGQWGPELGSIYILPALIGSIIFIAIVTLILRAVRKK"
     gene            complement(2258..2467)
                     /locus_tag="O0V33_001308"
     CDS             complement(2258..2467)
                     /locus_tag="O0V33_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975947.1"
                     /translation="MTTQSFIFMNIILLDGATQRHPFFICQILVFLFPTSSHVNLLLF
                     NPILPTFSNIIIHIDHFHANKKDDN"
     gene            2645..3226
                     /locus_tag="O0V33_001309"
     CDS             2645..3226
                     /locus_tag="O0V33_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCX8975948.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYEKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNKVSREEAVDLKVENCKPYIIE"
     gene            complement(3292..3675)
                     /locus_tag="O0V33_001310"
     CDS             complement(3292..3675)
                     /locus_tag="O0V33_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975949.1"
                     /translation="MKVRITFIILAILSTIVCLFLAAMHPTGPNTVTFEQPYLFTLNI
                     IIMVLVALPALILAIFNNMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFISAIITLAIGGKSFDKNDSH"
     gene            complement(3946..4572)
                     /locus_tag="O0V33_001311"
     CDS             complement(3946..4572)
                     /locus_tag="O0V33_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HCX8975950.1"
                     /translation="MKKRLLLSTFLASTLILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVNEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            complement(4645..6264)
                     /locus_tag="O0V33_001312"
     CDS             complement(4645..6264)
                     /locus_tag="O0V33_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975951.1"
                     /translation="MLDVHFIYPGKLQNACEYFNLPTQKEGFEVTIHALRPENIHKIY
                     HDMDKTEQKFIETLISNGNPVTIINKRDVNILKLTNHLGFVYQINEDEFFVNNLFLSY
                     INSEVLDSTYGEFESLDTINHMNLNEEVLRVRQHTRLNDVTLEHALLLKTNDELKTIC
                     RQYGIKGFSNKNKQNLVELIMTAFFNNNHIIEQVFSNTTLFEFDILSSILESDANYRL
                     NNELIEFEKLQLINFDNFLTSHFYYYYDFEYDCLILPNDVRKHIENFVNQVGNGDFEK
                     AVNQFVDEQTIENSLAEFENALELSFMEDEVDLDMLLEHDDFEDFGTTDSLRSDAITQ
                     LISKIKKGLIDKKDILPLRIILGSVNLYGILSTNHLLYLTQRFYDATFSKEELQYSLD
                     TLAGTELYYIEDDFVFHPVLFDVVESEASGIDISDEGYYVPASVEELIYYDRHHYLKS
                     DQAIKEFTSYLRKQINMKNDIEKEKYVNEIIMLLRTIPTSKLVQPIMQLFIDNAILNN
                     VQNDALLIEKATHARNHLRLWSLQGHRDIPN"
     gene            complement(6379..7902)
                     /gene="ahpF"
                     /locus_tag="O0V33_001313"
     CDS             complement(6379..7902)
                     /gene="ahpF"
                     /locus_tag="O0V33_001313"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCX8975952.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKELLTEIS
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKNLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGENHVTGICYEDMSTGEEHLLNLDGIFVQIGLLPNTSWLKDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            complement(7918..8487)
                     /gene="ahpC"
                     /locus_tag="O0V33_001314"
     CDS             complement(7918..8487)
                     /gene="ahpC"
                     /locus_tag="O0V33_001314"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCX8975953.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            8978..9733
                     /locus_tag="O0V33_001315"
     CDS             8978..9733
                     /locus_tag="O0V33_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HCX8975954.1"
                     /translation="MSDHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEQIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVARVREILDLPDYVFPLFG
                     MAVGEPAEDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISKYYDQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
     gene            complement(9813..11201)
                     /locus_tag="O0V33_001316"
     CDS             complement(9813..11201)
                     /locus_tag="O0V33_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCX8975955.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLVYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALMFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYIKKTLEVLIFAFTSRSSAGTLPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FESNDNAALTSH"
     gene            11286..11387
                     /locus_tag="O0V33_001317"
     CDS             11286..11387
                     /locus_tag="O0V33_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975956.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNSVVIGGKGK"
     gene            complement(12320..13276)
                     /locus_tag="O0V33_001318"
     CDS             complement(12320..13276)
                     /locus_tag="O0V33_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975957.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTTKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKVLAESSNKVENNTATDEKAESKDSKVAGVNTSTDAKTDTKAENAGDGTAT
                     KVDKEDQISATEAIEKASVEQPKNDKAAETSNKEATVDAEAQHDAEQQVAEAHAEASK
                     QATSNDSLEAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL"
     gene            complement(13394..14056)
                     /locus_tag="O0V33_001319"
     CDS             complement(13394..14056)
                     /locus_tag="O0V33_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975958.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYDLLEEMDLRK
                     PF"
     gene            complement(14199..14606)
                     /locus_tag="O0V33_001320"
     CDS             complement(14199..14606)
                     /locus_tag="O0V33_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCX8975959.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLH
                     DSEISLISTSGTFSDKMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVGV
                     RDHSSHQEVLENNSAYEEVDITHFAEASKGPKA"
     regulatory      14786..14888
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            15119..15697
                     /gene="xpt"
                     /locus_tag="O0V33_001321"
     CDS             15119..15697
                     /gene="xpt"
                     /locus_tag="O0V33_001321"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCX8975960.1"
                     /translation="MELLGQKVKEDGVVIDERILKVDGFLNHQIDAKLMDEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     gene            15697..16965
                     /locus_tag="O0V33_001322"
     CDS             15697..16965
                     /locus_tag="O0V33_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCX8975961.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILVLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFKFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            17003..18469
                     /gene="guaB"
                     /locus_tag="O0V33_001323"
     CDS             17003..18469
                     /gene="guaB"
                     /locus_tag="O0V33_001323"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCX8975962.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            18494..20035
                     /gene="guaA"
                     /locus_tag="O0V33_001324"
     CDS             18494..20035
                     /gene="guaA"
                     /locus_tag="O0V33_001324"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCX8975963.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCDCKGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
BASE COUNT         6746 a         3147 c         3726 g         6692 t
ORIGIN      
        1 aagtctctat aaaataactt agtttaaaaa cgattcgtat ctttcagatt caaataccat
       61 cattttctcc taagacttac actttaatta caattatgta agttgttttc agagacatcc
      121 tattttatat tttgtcagag gtgatctttt gaaaacgatt ttaaaaacaa taacatatct
      181 tgcatttact atcattggcg cttatgctgc tttattcatt ttaaaaacaa tagactctca
      241 tggtataaca gatcaattta acccattagt aaaggaagat gattcttatg ttaaaacgac
      301 agaggtgtct actagaatgg atgatcaact ccgaagttat agtcaaagtg cttttaataa
      361 agaagggaaa gagacgcaat taatgtatac tgctacattt gatgttaaac cgcatagata
      421 cttgaaaatt acacataaag gtcatcatgt agaaactttt gaagaagtag aaaaggaaga
      481 agtaccaaag aaagcattag acaaactgag tcgataatag cattcttata ttacatggtt
      541 cattttaaag atgagtacga aatgaagtga cacatgacga ttaatttaaa aatatttgta
      601 ataattatga atagaattaa aaacaagggg taatacaatc tatatagcat ataagctttt
      661 gttatgagtt tcaaaaaaag gaagagagag tgatattatg aaattaaaat cattagcagt
      721 gttatcaatg tcagcggtgg tgcttactgc atgtggcaat gatactccaa aagatgaaac
      781 aaaatcaaca gagtcaaata ctaatcaaga cactaataca acaaaagatg ttattgcatt
      841 aaaagatgtt aaaacaagcc cagaagatgc tgtgaaaaaa gctgaagaaa cttacaaagg
      901 ccaaaagttg aaaggaattt catttgaaaa ttctaatggt gaatgggctt ataaagtgac
      961 acaacaaaaa tctggtgaag agtcagaagt acttgttgat gataaaaata aaaaagtgat
     1021 taacaaaaag actgaaaaag aagatacagt gaatgaaaat gataacttta aatatagcga
     1081 tgctatagat tacaaaaaag ccattaaaga aggacaaaag gaatttgatg gtgatattaa
     1141 agaatggtca cttgaaaaag atgatggtaa acttgtttac aatatcgatt tgaaaaaagg
     1201 taataaaaaa caagaagtta ctgttgatgc taagaacggt aaagtattaa agagtgagca
     1261 agatcaataa aatatgccgt aacactaacg atggcaacat gtcttaattc atattaatta
     1321 ttaaaatacc ctcacaacgc aatgtaatat atcccgtata gatttgaaag gatatatgcc
     1381 taacgtgttg tgagggttta attgcatcta tttatccatg cgaatatcga cttcttctaa
     1441 atgtttctga tattctttaa ccttactttc taaaaacatt tcatatggtg catcaaagaa
     1501 gtcagctaaa tgcatggcat ctttaaattt aggttcatgg tgatgattct cccattccca
     1561 aatttgatgt gcttcatatt tagtaccata tttctcattt aattgttgtg ctaattcgtc
     1621 aatttctaaa ttatgtttag ttcgtaagtt atataaaata tgcatattca ttgttcatta
     1681 acctcgcttc ttgatttaaa attcaaagat gctcttataa tatgtgtata ttctagtcct
     1741 ataacataaa ataatcaagc tatgaattcg gcagaaaaaa taatgtatat gatattgccg
     1801 gtacagagac tttaaataac gatagctaca ttgaataaaa ttgatattca attactactt
     1861 ttaaaaatat ttggataaaa ataatttgaa tttgttttag aaagtctaat aatgggtact
     1921 acttagaata acgcaaaata aatagaaaaa ggagactgaa aattatgttt ggatttattg
     1981 gaatgttaat tgtcggtggc ttaattggat gggctgctgg tgctattatg ggtaaagata
     2041 tcccaggtgg tattttaggt aatattatcg caggtattat tggatcatgg gtaggtggca
     2101 aactattcgg acaatggggt cctgaattag gaagtattta catcttgcca gctttaatcg
     2161 gttcaattat ctttattgca atcgtaacgt taattttaag agcggttcgt aaaaaataaa
     2221 atattcaact taggttgaat taatataaag atggcattca gttgtcatct tttttatttg
     2281 cgtgaaaatg gtcaatatgt attataatat ttgaaaatgt aggtaagata ggattaaaaa
     2341 gtaataaatt aacatgagaa gaggttggaa ataaaaaaac gagtatttga caaataaaaa
     2401 aaggatgacg ttgggttgcg ccatccagga gtattatgtt catgaatata aaagactgtg
     2461 tagtcattta agaactacac ccatatagta acacatcact tgattaaaga caatactatt
     2521 tttagagtca ttaatttaaa atatgaatta attctttttt tagaatagaa tattaagatt
     2581 gatatcgaat tagtagtcaa agtgttatgg tagatatgaa atacataaga taaggagtaa
     2641 tattatgacg atttatttag ttagacatgg cgaatcaaaa tcgaattatg ataataaaca
     2701 ttttagatct tatttttgtg gacaattaga tgtgccgtta acggatactg gcacaaaaag
     2761 tgcggacgat ttatgtgatt attttaaaga gaaacagatt aaacatgtat atgtttcaga
     2821 cttattaaga acacagcaaa cgtttgaaca tatttttcca tatgacattg catcaacgac
     2881 tacccctcta ttaagagaac gttcacttgg cgtatttgag ggtgaatata aagatgaaat
     2941 cagtgcgaat ccgaaatatg aaaaatattt caatgatcca aactttaaag actttcgaca
     3001 tagtttttca caaaaagcgc ctgaaggaga aagttatgaa gatgtatatc aacgcgtaga
     3061 acattttatg aatcatgttg tcaatgaaga tacacaaaaa gatgatatcg tcattgttgc
     3121 acatcaagtt gtcattcgtt gtttgatggt ttattttaac aaagtttcaa gggaagaagc
     3181 tgtggattta aaggtagaaa attgcaaacc atatatcatt gaatagaagt atagaggttc
     3241 tgaaaagctg tgttttatag cggccttcag aacctttttt gtatttggat tttaatggga
     3301 atcattctta tcaaaagact tcccaccaat agctaatgtg ataattgcgc ttatgaacaa
     3361 aataattgaa gttataaatg acgctaatgt aaaaggtgta aattgtgtga gcgataatat
     3421 caatgataaa aagaaccaag agatagcccc tagaaattgt aaaatagcag aagaaattct
     3481 aaaactcata ttattgaata tagctaaaat aagtgcgggt aatgcgacta ataccattat
     3541 aatgatgttg agtgtgaata aatatggctg ttcgaaagtt actgtgtttg gacctgttgg
     3601 atgcattgct gctaagaaca agcaaacaat cgtcgataaa attgctaaaa taataaaagt
     3661 gattcgaact ttcatcatga ccatcctttg tttatagagt caatataagt atggaatatg
     3721 ttagggatat agtcaaatgc gtcaactaat ggggatttta ggatggatag agaatttaag
     3781 gcaattaaaa aagcatcaaa cagtaatatg ctgcttgatg caatatggta ttggttaaaa
     3841 tgtattgtct caaagattag tcacttttaa agataaagaa ttgtcatgaa ttaaagcgca
     3901 tgtactgatg agtgatgtgt tacatgtcgc aatgatggct ttcagttatt tatcgataac
     3961 atcactcttg atacctttag attttaagaa atctttaatt ttatcttgtt gctttttatt
     4021 aacatcaccg gcatattttg ttggcacgtc gactacattg attttatttt gcggttgata
     4081 gctaagcttt tcaatatctt cgttaacatt ggcgattgta ctatttaaag ctttgaagta
     4141 attcatcatt aattcaacgg gtttcttata ttctttagga atattgtttt cagtgacaaa
     4201 tttcttgaaa tgcaaatcat ttttaacagc taagttagat aagtggctaa gtgtttctgc
     4261 ttgtttttca gtcacttttg tttgactgtc aatttgttta tctagtttat gttgcataat
     4321 atatttgtta tcaagtatat cgctatttac agacaaatac ttttctatag cttgcttcat
     4381 ctctgcatca ctaatatcac tatttttctt atctgagtta aaaatatctt ttgtttctaa
     4441 ttttttagcg cttttaggtg catggatgcc agtacttgtg tgatgatctt cgttatcaga
     4501 ttgatcggac gcgcaacctg taagaattaa tgtcgatgct aaaaatgtac ttagtagtaa
     4561 tctctttttc ataatgtaat ataactcctt agtttatctt taattgaaaa aatatgtatt
     4621 catgtttaat agagtaacat tgaattagtt tggaatgtca cgatgacctt gcaatgacca
     4681 tagacgtaaa tgattacgtg catgagttgc tttttctatc aataatgcgt cgttttgaac
     4741 gttgttaagg atagcattat ctataaataa ctgcataatt ggttgtacta atttagacgt
     4801 aggtattgta cgtaaaagca taatgatttc attcacatac ttttccttct caatatcatt
     4861 tttcatattg atttgtttgc gtagataact ggtaaactct ttaattgctt gatcagattt
     4921 caaatagtgg tgtctatcat aatagattaa ttcctcaaca gaagctggta catagtatcc
     4981 ttcatcagaa atatcaatgc ctgacgcctc agactcgaca acatcaaata acacagggtg
     5041 aaacacgaag tcatcttcaa tataatatag ctcagtacca gctaaagtat ctaagctata
     5101 ttgcaactct tctttactaa aagtagcgtc ataaaatctt tgtgtgagat atagtagatg
     5161 attagttgat aaaataccat acaagtttac agaaccaagt ataatacgaa gaggtaaaat
     5221 atctttttta tctattaatc ctttcttaat cttagaaatc aattgagtga ttgcatcact
     5281 tcttaagcta tcggttgtac caaaatcttc aaaatcgtca tgttcaagta acatatctaa
     5341 gtcaacttcg tcttccataa atgataattc taacgcgttt tcaaactctg ctaaactatt
     5401 ctcaattgtt tgctcatcaa caaattgatt tacagctttc tcaaaatcac cattgccgac
     5461 ttgattcacg aaattttcaa tatgtttacg cacatcattt ggcaaaataa gacaatcata
     5521 ttcaaagtcg taataataat aaaaatgact agttaaaaag ttgtcaaaat ttattaattg
     5581 tagcttttca aattcaatta gttcattgtt taatcggtag ttagcatcac tttcaagtat
     5641 gctagaaaga atgtcaaact caaatagagt agtattagaa aacacttgtt caatgatgtg
     5701 attattattg aaaaatgctg tcataattaa ttcaacaaga ttttgtttgt ttttatttga
     5761 gaatcctttg ataccatact gcctgcatat tgtttttagc tcgtcattag tttttaacag
     5821 taaagcatgt tctaatgtga catcgtttaa gcgcgtatgt tgtcttacac gtaacacttc
     5881 ttcatttaaa ttcatatggt ttattgtatc taacgattcg aattcaccat aggtactatc
     5941 tagtacttct gaattgatat aacttaaaaa taaattattt acaaagaact catcttcatt
     6001 aatttgataa acaaaaccta gatgatttgt tagctttaaa atattaacat cgcgtttatt
     6061 tataattgtg acaggattac cattagatat aagtgtttct ataaattttt gttctgtctt
     6121 atccatatca tgataaattt tatgtatatt ttcagggcgt aaagcgtgaa tcgtgacttc
     6181 aaatccttct ttttgggtcg gtaaattaaa atattcgcat gcattttgta attttcctgg
     6241 gtatataaaa tgaacatcaa gcatttttgc acacctcgtt gtatatatta agtttatcat
     6301 aacatgattt tatgtcggga taaaaaaata acagcatctt aacaaatgta agatactgtc
     6361 agtgaaatga atgaaacttt agtttctgat aatatagtca aaggcattta atgctgcatt
     6421 tgcaccagcg cccattgaaa taataatttg tttgttcttc tgatctgtga catcgccagc
     6481 agcaaatatt ccaggtacgt tagtattatt gttacgatca ataacaattt caccacgttc
     6541 gtttaattca acagcatctt ttaaccatga tgtgtttgga agtaaaccaa tttgaacaaa
     6601 gataccatct aagttaagta gatgttcttc gccggtgctc atgtcttcgt aacatatacc
     6661 tgtaacatgg ttttctccga caacttcagt agttttggca tttgttttga tatcaacatt
     6721 tgataaagaa cgtaaacgat cttgtaacac gttgtctgct tttaattcgc tagcgaattc
     6781 gaataatgta acatgattaa cgataccagc aaggtcaatt gctgcttcaa ccccagagtt
     6841 accgccaccg ataactgcta cgtctttatt ttcaaataga ggtccgtcac agtgagggca
     6901 gaatgcaaca cctttattaa tcaattgctc ttcacctgga atgtttagct tacgccaacc
     6961 tgcaccagta gcaataatga ctgttttact ttctaggaca gcaccgtttt ctaacgtaac
     7021 tttaattgct tcgtcagtct tttcgatatc tgtagcacga atgcctgtca ttgcatcaat
     7081 gtcatattga tcaatgtgcg ctgctaagtt agaagaaaat tcagaaccag ttgtttcttt
     7141 aacagtaatg aagttctcaa taccagcagt atcattaact tggcccccga tacgatcagc
     7201 aactatacca gtacgtaaac ctttacgtgc tgtgtaaatc gctgcactac cactagcagg
     7261 accaccacca acgattaaga catcataagg ttctttgttt tcaaactcag atgcatctgc
     7321 cgtactgcct agtttcgaaa gaatatcttg gattgtcata cgaccattgc caaattcttc
     7381 gccatttaaa aagacagcag ggactgccat gatgttttca gattcttcac ggaacactgc
     7441 accatcaatc atagaatgcg tgatgttagg gttaatcaca ctcattaagt taagtgcttg
     7501 aacgacatca ggacattttt gacacgttaa actaatgaat gtttcaaaat ggaatgaacc
     7561 ttctaagttt ttaatttggt caatgattga ctgtttttct ttaggtgcac gaccactaac
     7621 ctgtaaaatt gctaaaacaa gtgagttaaa ctcgtgacct aatggaatac ctgcaaatgt
     7681 tacacctgtt tcttcgccag gacgattgac tgagaaactt ggtgtacgtt ttaaagactt
     7741 ttcagaaagt gaaagtctag gtgacatatc agaaatttct gttaataatt ctttgagttc
     7801 cttagattta tcatctgaac caaggctggc aacgaattca acgttgccct ccattagttc
     7861 taatagttgt ttaagttgtt gttttaaatc agcattaagc atggttgtaa tgcctcctta
     7921 gattttacct actaaatcta aaccaggttg caatgtttta gcgccttctt cccatttagc
     7981 tgggcatact tcgccagggt ttttgcgaac atattgagct gctttgattt tgtgagctaa
     8041 tgtactagcg tcacggccaa ttccgtcagc gttaatttca gatgcttgta caacaccgtc
     8101 tgggtcgata atgaatgtac cacgttgagc taaaccagta gcttcatcta atacatcaaa
     8161 attacgagtg attgtttgtg atgggtcacc aatcatagtg taagtgattt tgctaattgc
     8221 atctgaatgg tcatgccatg ctttgtgtac gaagtgagta tcagttgata ctgagaatac
     8281 atttacgcct aatttttgta attcttcata ttggttttgt aagtcttcta attcagttgg
     8341 acaaacgaat gagaagtcag caggatagaa gcatactacg ctccaagaac cttttaaatc
     8401 ttcttgtgta acttctttaa attgatcttt ttttggatcg aaagcttgcg ctgtaaatgg
     8461 taagatttct ttgttaatta atgacataaa tatcttcctc ctaagaattt aagtatgaat
     8521 tagaactatc aattgattgt gcttaattat aataattcta atctcttagt tagcattatt
     8581 acattttgat ccagaatagt caactggata actttgtaaa gtgaatgatt acttttaaaa
     8641 taaagaaaga taatataaag tgctttgata atggattttg tagtcgatga tttaaaaggt
     8701 tgtgtctata tttaatatct tgattttaat gtaaaaaatg taaaaaaaga agatttgtat
     8761 tctcaactaa gtcaacctta ttgataatgg tgtgagaata tttgttcgag atggatgaag
     8821 gtaatgcgtg agaaactgga ttttttaagt acgagacaat atttttgaaa gttcaattat
     8881 taacttataa gcaaataatt gctataaaaa agtttggacg tgtacaattg caatatgaag
     8941 atttttaatt aactgtaaag tatcgaggag tggtaacgtg tcagatcatg tatataatct
     9001 tgtgaaaaag catcattctg ttagaaaatt taagaataaa cctttaagtg aagacgttgt
     9061 taagaaattg gtagaagctg gacaaagcgc ttcgacgtca agtttcctac aagcatactc
     9121 aattattggt atcgacgatg agcagattaa agaaaattta cgagaagttt ctggacaacc
     9181 ttatgttgta gaaaatggct atttattcgt ctttgttatt gattattatc gtcatcattt
     9241 agttgatcaa catgctgaaa ctgatatgga aaatgcatat ggttcaacgg aaggtttgct
     9301 agtaggtgca atcgatgcag cattagttgc cgaaaatatt gcggtaactg ctgaagatat
     9361 ggggtatggc attgtctttt taggatcatt gagaaatgat gttgcacgag ttcgagaaat
     9421 tttagactta ccggactatg tctttccatt atttggtatg gcagtagggg aacctgcaga
     9481 agatgaaaat ggcgcagcca aaccacgctt accatttgac catgtcttcc atcataataa
     9541 gtatcatgct gataaggaaa cacagtatgc acaaatggca gattacgatc agacaatcag
     9601 caagtactat gatcaacgta caaacgggaa tcgcaaagaa acatggtcgc agcaaattga
     9661 gatgttccta ggaaacaaag caagattaga tatgttagaa caattgcaaa aatcaggctt
     9721 aatacagcga tagcaagata ccaaaataac ccgccccctc tagcttaaaa tgataagtat
     9781 agctagaagg ggcgggtatt tcttgcaatg aattagtgtg aagttaatgc agcattatca
     9841 tttgattcga aagtatcttt gtcccaatgt ttagttaact tggcggtacc tgtaccagct
     9901 agcattgaat cgttcacgtt taatgctgtt ctacccatgt caatcaatgg ttcaacggag
     9961 atgagcacgc cggctaaagc gactggcaag tttaacgttg acaacaccaa tatggatgca
    10021 aatgtagccc cgccaccgac gccagcaacg ccgaatgaac taataatcac gacagcgatt
    10081 aacgttacaa taaattgtaa atcaatttct acattagcga cgggtgcgac cataattgca
    10141 agcatggcag gataaatgcc agcacaacca ttttgtccaa tcgacaatcc aaatgtcgca
    10201 gcgaaattgg caataccctc cggcacgcct agacgtcttg tttgtgtttg tacattcagt
    10261 ggtaaggtac ccgcgcttga gcgtgatgtg aatgcaaaga ttaatacttc caaagtcttt
    10321 ttaatatagc gaatagggct aatgcctaac aggcttaaaa taattaagtg aatgatatac
    10381 atcgtaatta atgcagcgta cgatgcgatt aagaattttc ctaaagtcca aatggcgcca
    10441 aagtcacttg tcgataacgt gttggccata attgctaata caccgtatgg cgttaaacgt
    10501 aagacgaacg tcacaatcgc cattactagt gaatagatag cgtcaatcgc acgcttaagc
    10561 aattcaccat gatcaggttg tttgcgtgct acgcgtaaat aagcaaatcc tataaacgaa
    10621 gcaaatatca caacagcaat cgtggaagtt gcacgttgtc cagtgaaatc taagaatgga
    10681 tttttaggca ataattccaa aatttgttgt ggtaacgtat gtgctgttaa atctttcgct
    10741 tgtttagcaa tttcgcttcc acgtgcttgt tcagcgttac caagattaat tgttgatgcg
    10801 tctaaaccaa acatcaaggc atacacaaca ccgacaattg cagcaatggt gacagtgccg
    10861 attaaaaaga taaaaatgag actaccaatt ttagcaaatt tttctccgat ttgaatttta
    10921 gtgaatgcag cgacaataga aatgaaaatt aaaggcatca caatcatttg caacaatgca
    10981 acgtaacctt gtccgacaat gttgaaccag tcacttgttg atgttataac attcgaatgt
    11041 gttccataga caagatgtag taacacgcca aatacaatgc cgatacctaa agctgtaaac
    11101 acacgtttcg caaaagatat atgtttacga gccatcatgt gcaatattac gatgaaaatc
    11161 accaatacaa taatattaat cagtgtaaga aaagcattca tgaacgtcac tcctcaaatt
    11221 tttgaatata attccgacca gtatactaag aataatatta attttaaatt atttcaagag
    11281 ggcatttgca attaaatgta aacatgatga aattcgaccg ttggcataag gggatttgta
    11341 aacaaatcgt tttgaattca gtagtgatag gtggaaaggg taaatagtgt gatgagctag
    11401 tcgattaaag ttaatatttg aaaatgaagc agggcattaa atgcaataaa ttaaataagt
    11461 tgtcattaca gcgttaataa tagaaatgat tttaacagga aaaaagtgat gaatatttgg
    11521 aaaagatata tatcgtgtac tgtcatgaga gataagatat gagaaaacaa atgtatcctt
    11581 gaatcgagat gtggctatgg cggtttgata aaaattgcta acctcatatt tattgaataa
    11641 aaaagataat aaatgatcaa ccttgaaaag acgcttcgaa caaacacgtt gtaaaaaatt
    11701 ctttcgaatc aaaattagca tcttttgagg attcgtgtgt gcaggtggta gttcaaaagc
    11761 taccgtaaac aaatattttt gtttgagcaa aaaggcgttt gtagtaaatg tgaatatgag
    11821 tgtgcccttg tgcgattcgt catttactaa cacagccaaa catttaaatt gaatgggctt
    11881 attaagatga atctatttag aagtacacag aagccatccc atttaaaagt aacatactac
    11941 aaaaatttat tcaaaattca atattaatca taatgaataa ttaaaaaacc tgaaacaaat
    12001 tagtttgata tgctctcttc aaagtagata ttgaaaggag tttatcagat tgtttcaggt
    12061 tttatgaatt gagtattaaa tttgaaaata aattacagtt ctatagtttt ataaaagatc
    12121 agtttttaaa tcatagatat atattactga gtcttacagg ctgggacata aatcaatgtt
    12181 ctatgctcta cgaagttata ttggcagtag ttgactgaac gaaaatgcgc ttgtaacaag
    12241 cttttttcaa ttctagtcaa ccttgccggg gtgggacgac gaaataaatt ttgcgaaaat
    12301 atcatttctg tcccactccc tacaggttga ataaacgtgc gaagaaacct tttttctctt
    12361 cttgaggttt tggttctgac atttcagact gtgaagctgt gctgtcattt tctgctttcg
    12421 cttccaatga atcgttcgaa gtagcttgtt tagaagcttc tgcatgggct tctgctactt
    12481 gttgttcagc atcatgttgc gcttctgcat ctactgtagc ttctttattt gaagtctcag
    12541 ctgctttgtc atttttaggc tgctcaacgc tggctttttc aattgcttca gttgcagaaa
    12601 tttgatcctc tttatctact ttagtagcag tgccatcgcc tgcattttct gctttagtat
    12661 ccgtcttagc gtctgttgat gtattcactc cagcaacctt cgaatcttta ctttcagcct
    12721 tttcatcagt agcagtatta ttttcaactt tatttgatga ttcagccaat actttagctt
    12781 gagatgcttg tgcttcttgg atagcctctt gtgatgtttg gatggctttt aaggattcgc
    12841 tatttgaatc gattttagtt gtaagttgat tttgaagttc ttttaattct tgttgttgct
    12901 gatgtacttg attcatcatt tgaccaagta ggtgacgttc ttcgcgcatt ttctggattt
    12961 ctagacgcaa atcttctact agttgagcaa cattttgatt agtaggtaag tttttgtcat
    13021 cgtttttgac aatgacttgc aggaagtctt tttctttttc taattcatca aacgctagat
    13081 cataactatt tgtttgtttt actttgtcgg ctatgtcttt gaaaagctcg atatcttctt
    13141 ctttgaaatc agttgcttca cgaccacgat attcagtctt gcttagttgg taaccacgtt
    13201 cttctaaatg ttgaacaatt ttacggactt gcttctcact tagttctacg cgttgtgcaa
    13261 attctttagt taacatactt taaagtcctt tctgcatata ctttatgtat actgacgttt
    13321 gtctacgggc agtgaaacgt cagtcttgct tcatacatag tttaaacgtt attgttagtg
    13381 atttcaagtt tgtttaaaat ggtttacgta aatccatttc ttctaataaa tcataaataa
    13441 tttcacgtgc gataccattt gcaaatacat acttcaaaat ataatttgat ttaacacttg
    13501 taccgtagat atcctttgtc agtttaatat ttttctttaa aaataagtcc acttttttag
    13561 tgtgaccatc attagttgga tgaatgtaat caatttcagt ttgtctttca aattgatgtt
    13621 cgtataaaaa ctgtctacgg tgttcaataa tgagcccatt atcttgtgtt aaatctgcag
    13681 gttctttagg gacttccatc attaaaaagt taatacttct agaatgaaca aggtttgata
    13741 ataagtttaa ttcatgttca atttgatgta atgtttgtgt cattaactca tcatggttgg
    13801 gtaggtcttt tgcaaataaa taaattaaaa atgcagaatt ggttgtggct tcataatgtg
    13861 cagttgctag actgacaaca acatcgtttt ctaagcctac gataaagaca taatcgtttt
    13921 cagttttgtt gttttctaat gagcgtttga tgatacgttt atcttcagtt aaagataaat
    13981 ttaacttagt atcgtaaagt ttaagtgctt cgttgtaatg tggatctttg acagattgaa
    14041 tggttttaaa ttccataaga acacctcccc aatttaaata atattatagc ataatcgcct
    14101 gttgtaaaag actgttcata aacttttaaa tggtataaaa aactgtacta tcttaaatta
    14161 gacagtacag taatctcatt ttgaattcag tgtgataact aagctttggg acctttagat
    14221 gcttcagcaa aatgtgtaat atcaacttct tcataagctg aattattttc tagtacttct
    14281 tggtgagatg aatgatcgcg aacgccaaca actaacattt tatcgtctaa aataagttgt
    14341 ttatattttt ctaattcatc aggcgctaag ttgtagcgtg ataaaactgc atgttcacca
    14401 tcttctcctg ttaacagttt agtcatttta tcactaaatg ttccacttgt tgagataagg
    14461 gagatttcag agtcgtgtaa gtcatttagg tgtaatttac ttttactaat aattgttagc
    14521 tctgattcta aataaccttc agatttcttt tgattgatta cgttgtataa ttcgccagtg
    14581 tcatttacta cagtaatatc tgccatagtt gtcgcccctt taaaaatttg tttattcaat
    14641 cttttaccct tcttattata aagtaaaacc cttacattat taagttataa gtcttcattc
    14701 gcattaaacg tctctgtaca tttataaaac tattaaaagt ttacctaagc agttattgaa
    14761 aaaatgccga aaatttgcta ttatcgttaa ataatttaca taaactcata taatctaaag
    14821 aatatggctt tagaagtttc taccatgttg ccttgaacga catgactatg agtaacaaca
    14881 caatactagg agtagcttca gccattaaat tgtaaccatg atgggtgatt tatatcattt
    14941 tatatgatgg tcacagttta tttgatgaaa cttcttttac attgattgca tgaccaatac
    15001 gtgatgcatg ttcgttcact cataaaccct gaaactatta tttagtttgg ggattttttt
    15061 gtatctagca ccaatttaag agcaaaatgt ttcacacaaa tctgaggagg ttttaagagt
    15121 ggagttacta ggacaaaaag taaaggaaga cggcgttgtc attgatgaga ggattttaaa
    15181 agtcgatgga tttttaaatc atcaaattga tgcaaagtta atggatgaag ttggtcgcac
    15241 tttttacgag caatttaaag ataaagggat tactaaaatc ttaaccattg aagcttccgg
    15301 tatcgcacca gcaatcatgg ctgcactgca ttttgatgtg ccatgtttat ttgcgaaaaa
    15361 agcaaaacct agcactttga cggatggtta ttatgaaaca tctattcatt catttactaa
    15421 aaataaaaca agtacggtca ttgtttcaaa agaattttta tcagaagaag atactgtact
    15481 catcatcgat gactttttag caaatggtga tgcttcatta ggattatacg atatcgcaca
    15541 gcaagcgaat gctaagacag ctggtattgg tatagttgtt gaaaagagtt tccaaaatgg
    15601 gcatcaacgt ttagaagaag caggcttaac agtttcttct ctctgcaagg ttgcttcact
    15661 agaaggaaat aaagtgacat tggtgggaga agaataatga aaaatttaat cctaagtgtt
    15721 caacatcttt tagctatgta cgcaggtgcc atcttagttc caatcattgt tggtacaagt
    15781 ttgaagttta cgcctgaaca aatcgcttac ttagttacag tagatatatt tatgtgtgga
    15841 gttgccacat ttttacaagc caataaagta acaggaacag gattaccaat cgttcttgga
    15901 tgtacattca cggctgttgc gcccatgatt ttaattggtc aaacgaaagg aatagatgta
    15961 ctttacggtt cgctattttt atcagggata ttagttatta tcatcgcgcc tttcttttca
    16021 catcttgtga aattcttccc accagtagta acgggtagtg ttgttactat cattggtatc
    16081 aatttaatgc cagtagcaat gaattactta gctggaggtc aaggtgcaaa ggactatgga
    16141 gatgttaaga acattttgtt aggtttaatg acattaatca ttattcttgt tttacaaaga
    16201 ttcacaactg gatttattaa gagtattgcc atattaattg gactcgtttt aggaacgata
    16261 ggtgctggct tacttgggat ggtcgatatt aatcaagtca atcatgccgg ttggttaggc
    16321 atcccagtgc cgtttaaatt ctcaggattt agctttgatg tgacatcgac gttagtgttc
    16381 tttattgtag ctatcgttag tttaattgag tcgacaggtg tctatcatgc gttaagtgaa
    16441 attaccggta agaagttaga aagaaaagat tttcgtaaag gttatactgc ggaaggtcta
    16501 gcgatagtgt taggttctat attcaattca tttccgtata cagcctattc gcaaaatgta
    16561 ggacttgttt ctttatccgg cgctaagaaa aacaatgtta tatacggcat ggtcgtgtta
    16621 ttacttatat gtggttgtat acctaagctt ggcgcattag caaatatcat accgctacct
    16681 gtgttaggtg gtgcgatgat agctatgttt ggcatggtaa tggcatatgg tgttagtata
    16741 ttaggacata tcgattttaa aaatcaaaac aatttattaa ttatcgctgt atcagtagga
    16801 ttaggtactg gtataagcgc tgtaccacaa gcatttaaag gtttaggtga acaatttgca
    16861 tggttgaccc aaaacggaat tgttttaggc gcaatctctg caattattct taatttcttt
    16921 tttaatggaa taaagtataa acaaacggaa gaaaatgtga aataatataa ctaattaatt
    16981 tgaaaaatgg aggctgtttt taatgtggga aagtaaattt gcaaaagaat cattaacgtt
    17041 tgatgatgtg ttattaattc cagcacaatc tgatatttta ccgaaagacg ttgatttaag
    17101 cgtacaatta tcagacaaag ttaaattaaa tattccagtt atttctgctg gtatggatac
    17161 tgtaactgaa tctaaaatgg cgattgctat ggctcgtcaa ggtggtttag gtgttattca
    17221 taaaaatatg ggcgttgaag aacaagcgga cgaagttcaa aaagtaaaac gctcagaaaa
    17281 tggtgtcatt tcaaacccat ttttcttaac accagaagaa agtgtttatg aagcggaagc
    17341 attaatgggt aaataccgta tttcaggtgt accaattgtt gataataaag aagatcgcaa
    17401 cttagtaggt attttaacaa accgcgactt acgttttatt gaagacttct caattaaaat
    17461 tgtagatgta atgacgcaag aaaatttaat tacagctcca gtgaatacaa cacttgaaga
    17521 agcagaaaaa attctccaaa aacataagat tgaaaagtta ccattagtta aagacgggcg
    17581 tctagaaggt cttattacta ttaaagatat tgaaaaagtt atcgaattcc ctaatgcagc
    17641 aaaagatgaa catggtcgtc tacttgtagc cgcagcaatc ggtatttcaa aagacactga
    17701 tattcgtgct caaaaattag tcgaagcagg tgtggatgtc ttagttatcg atacagcaca
    17761 tggtcactct aaaggcgtta tcgatcaagt gaaacatatt aagaagactt acccagaaat
    17821 cacattagta gcaggtaacg tagcaactgc agaagcaaca aaggatttat ttgaagcggg
    17881 tgcagatatt gttaaagttg gtattggccc aggttcaatt tgtacgacac gtgttgtagc
    17941 aggtgttggt gtaccacaaa ttacagcaat ttatgattgt gcaactgaag cacgcaaaca
    18001 tggtaaagct atcattgctg atggtggtat taaattctca ggagatatca ttaaagcatt
    18061 agctgctggt ggacatgcgg ttatgttagg tagcttatta gcaggtactg aagaaagccc
    18121 aggcgcaaca gaaattttcc aaggtagaca atataaagta taccgcggta tgggctcttt
    18181 aggtgcgatg gaaaaaggtt caaacgaccg ttacttccaa gaggacaaag cgcctaagaa
    18241 atttgtacct gaaggtatcg aaggacgtac agcatataaa ggtgcgttac aagatacaat
    18301 ttaccaatta atgggcggtg tgcgtgctgg tatgggttat actggttcac acgatttaag
    18361 agaattacgc gaagaagcac aattcacacg tatgggccct gctggtttag cagaaagtca
    18421 cccacataat attcaaatta cgaaagaatc accgaactac tcattctaat taagataaag
    18481 gagaacgaca aatatggaaa tggcaaaaga acaagagtta atccttgtct tagactttgg
    18541 tagccaatac aaccaattaa ttacacgccg aattcgtgaa atgggcgttt atagtgaatt
    18601 acacgatcat gaaatttcaa ttgaagaaat taagaaaatg aatccaaaag gtatcatctt
    18661 atcaggtggt ccaaattcag tttatgaaga aggttcattt acaattgatc cggaaatata
    18721 taatttagga attccagtac ttggtatttg ttacggcatg caattaacta ctaaattatt
    18781 aggtggtaaa gttgaacgtg ccaatgaacg tgaatacggt aaagcaatca ttaatgcgaa
    18841 gtcagatgag ttattcgctg gcttaccagc agaacaaact gtttggatga gtcattctga
    18901 taaagttatt gaaattccag aaggctttga agttatcgct gatagtccaa gtacagacta
    18961 tgcagcaatc gaagataaga aacgtcgcat ttatggtgtt caattccatc cagaagtacg
    19021 tcatacagaa tatggtaatg atttattaaa taattttgtc cgtcgtgttt gtgattgtaa
    19081 aggtcaatgg acaatggaaa actttatcga aatcgaaatt gaaaagattc gtcaacgcgt
    19141 aggagaccgt cgtgtattat gtgcgatgag tggcggcgta gattcatctg ttgtagctgt
    19201 actattgcat aaagcaatag gtgatcaact aacatgtatc tttgtagacc atggcttact
    19261 tcgtaaaggt gaaggcgata tggttatgga gcaattcggt gaaggtttca acatgaatat
    19321 tatccgtgtt aatgcgaaag atcgctttat gaataaatta aaaggtgttt cagatcctga
    19381 acaaaaacgt aaaatcattg gtaatgaatt tgtatatgtg tttgatgatg aagcatcaaa
    19441 acttaaaggt gtagacttcc ttgcgcaagg aacactatat acagacgtca tcgaatcagg
    19501 tactaagaca gcacaaacaa tcaaatcaca tcacaatgtt ggtggattac cagaagacat
    19561 ggaattcgaa ttaatcgaac caatcaatac attgtttaaa gatgaagtac gtaaattagg
    19621 tattgagtta ggtattccag aacatttagt atggcgacaa ccattcccag gacctggtct
    19681 tggtattcgt gtacttggag aaattactga agataaacta gaaatcgtaa gagaatcaga
    19741 cgcgatttta cgccaagtga ttagagaaga aggtcttgaa agagaaattt ggcaatactt
    19801 cacagtgtta ccaaacattc aatcagtagg tgttatggga gactaccgta cgtatgatca
    19861 cacagtaggt atccgtgcag taacatctat cgacggcatg acaagtgact tcgcacgcat
    19921 cgattgggaa gtcttacaaa agatttctag tcgtatcgta aacgaagtag atcacgtcaa
    19981 ccgcgtagtc tatgacatta catcaaaacc accaagcaca attgagtggg aataattata
    20041 tatagcaggc tgggacataa atcaatgttc tatgctctac gaagttatat tggcagtagt
    20101 tgactgaacg aaaatgcact tgtaacaagc ttttttcaat tctagtcagg ggccccaaca
    20161 aagagaaatt ggatttccaa tttctacaga caatgcaagt tggggtgtgg gccctaacac
    20221 agaaaattca ttttattgaa ttttacattt atgtgcaagt tgggcaaagt gtcttatttt
    20281 ttcaaagtat ttaaaagtaa aattacatgt t
//
LOCUS       DAKSQE010000044        19995 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.044, whole genome shotgun sequence.
ACCESSION   DAKSQE010000044 DAKSQE010000000
VERSION     DAKSQE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19995
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.044"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(45..692)
                     /locus_tag="O0V33_001325"
     CDS             complement(45..692)
                     /locus_tag="O0V33_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCX8975964.1"
                     /translation="MKFENYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSAQDISH
                     YMGIPIEESFLKLADRPLDEATLAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     QQTVYIGDSTFDVEMAQHAGVPSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
     gene            complement(805..1353)
                     /gene="hxlB"
                     /locus_tag="O0V33_001326"
     CDS             complement(805..1353)
                     /gene="hxlB"
                     /locus_tag="O0V33_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HCX8975965.1"
                     /translation="MAKFSDYQLILDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEHGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVSEQTMQQNHANLE"
     gene            complement(1355..1987)
                     /gene="hxlA"
                     /locus_tag="O0V33_001327"
     CDS             complement(1355..1987)
                     /gene="hxlA"
                     /locus_tag="O0V33_001327"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HCX8975966.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIMDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKEIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            complement(2064..2822)
                     /gene="nagB"
                     /locus_tag="O0V33_001328"
     CDS             complement(2064..2822)
                     /gene="nagB"
                     /locus_tag="O0V33_001328"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCX8975967.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDAYDMNAEASTYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFENEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
     gene            3099..3461
                     /locus_tag="O0V33_001329"
     CDS             3099..3461
                     /locus_tag="O0V33_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HCX8975968.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQVTYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEINEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
     gene            3475..4140
                     /gene="bshB2"
                     /locus_tag="O0V33_001330"
     CDS             3475..4140
                     /gene="bshB2"
                     /locus_tag="O0V33_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HCX8975969.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLASYIQKGTPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERIPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            4153..5031
                     /locus_tag="O0V33_001331"
     CDS             4153..5031
                     /locus_tag="O0V33_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HCX8975970.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLCTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQDIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLEGFDIECRNE
                     ESIHQHDAFAKLKYRK"
     gene            5313..6803
                     /locus_tag="O0V33_001332"
     CDS             5313..6803
                     /locus_tag="O0V33_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCX8975971.1"
                     /translation="MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPVQCVFLSWNRY
                     LYRNAQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRI
                     YDQERFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLC
                     EFFYNPEGDTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGD
                     LFFSDRNVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYR
                     AIIVSTEQQKLDVEKRINHTIPVVNIPVGYSETIDTPVQTLDQHSVKLISVARYSPEK
                     QLHQQIELVKRLVPYVPKVEIHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQE
                     YSDAYLSLITSNMEGFSLALLESLAHGVPVISYDIKYGPGELITSDFNGYLITKNDED
                     ALFDKVKYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
     gene            complement(6929..8401)
                     /locus_tag="O0V33_001333"
     CDS             complement(6929..8401)
                     /locus_tag="O0V33_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCX8975972.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDLFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFIMYAHFLDKQYHQLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVIVVLHSTHLSGTGNDIKNFYKTVFNNLTRYKAIVV
                     STEKQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKDKNHIISIARLVENKQITH
                     QIEVIKQLVTKHPKIQLNIYGHGNGLSEYRQLVEGYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDVDYGPSELIQDGFNGYLVPPGDINQMVE
                     KVDQLLNNSQMMQQFSINSIESAQQYNATTISTKWQKILN"
     gene            complement(8520..11771)
                     /locus_tag="O0V33_001334"
     CDS             complement(8520..11771)
                     /locus_tag="O0V33_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046462978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HCX8975973.1"
                     /translation="MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAENTSTENAKQDEASASDNKEVVSETENNSTQKNDLTNPIKKETNTDSHQ
                     EAKEAPTTSSTQQQQNNATTSTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNN
                     TNNDVTTKPSTSEIQTTPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPVNVSKE
                     ELKNNPEKLKELVRNDSNTDRSTKPVATAPTSVAPKRVNAKIRFAVAQPAAVTSNNVN
                     DLITVTKQMITEGIKDDGVIQAHDGEHIIYTSDFKIDNAVKAGDTMTVKYDKHTIPSD
                     ITDDFTPVDITDPSGEVIAKGTFDLNTKTITYKFTDYVDRYENVNAKLELNSYIDKKE
                     VPNETNLNLTFATADKETSKNVKVEYQKPIVKDESNIQSIFSHLDTTKHEVEQTIYVN
                     PLKLNAKNTNVTIKSGGVADNGDYYTGDGSTIIDSNTEIKVYKVASGQQLPQSNKIYD
                     YSQYEDVTNSVTINKNYGTNMANINFGDIDSAYIVKVVSKYTPGAEDDLAVQQGVRMT
                     TTNKYNYSSYAGYTNTILSTTDSGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQGTDSK
                     EKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPAGYLPTKENGT
                     TDGEKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDANEPGIK
                     DVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTTAEDKD
                     SNGLTTTGVIKDADNWTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKDVTVTL
                     QNEKGEVIGTTKTDENGKYHFDNLDSGKYKVIFEKPAGLTQTGTNTTEDDKDADGGEV
                     DVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHTPV
                     KPMSATKDHHNKAKALPETGSENNGSNNATLFGGLFAALGSLLLFGRRKKQNK"
     gene            complement(12168..15296)
                     /locus_tag="O0V33_001335"
     CDS             complement(12168..15296)
                     /locus_tag="O0V33_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HCX8975974.1"
                     /translation="MNNKKTATNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLS
                     GHEAKAAEHTNGELNQSKNEATAPSENKTTEKVDSRQQNNVEQSTTSNQPKVNESDNT
                     SVKETTEEPQNTTSTQPTKKNNDATANKDNLAAQNISTQANDVSATPKTTTIKPRTLN
                     RMAVNTVAAPQQGTNVNDKVHFSNIDIAIDKGHVNSTTGKTEFWATSSDVLKLKANYT
                     IDDSVKEGDTFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDSTTNTTTYTFT
                     NYVDQYTNVSGSFEQVAFAKRENATTDKTAYKMEVTLGNDAYSEEIIVDYGNKKAQPL
                     ISSTNYINNEDLSRNMTVYVNQPKNTYTKETFVSTLTGYKFNPDAKNFKIYEVTDQNQ
                     FVDSFTPDTSKLIDVTDKFKITYSNDNKTATVDLMNGQTNSNKQYIIQQVAYPDNTST
                     DNGKIDYTLDTDKTKYSWSNSYSSVNGSSTANGDQKKYNLGDYVWEDTNKDGKQDSSE
                     KGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEFETPSGYTPTQVGSGT
                     DEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTNKNGVQDKDEKGISGV
                     TVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGYTPTSVTTGNDTEKDS
                     NGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKDVKVTLL
                     NEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTGTNTTEDDKDADGGEVD
                     VTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSESDSDSDSESDSDSDSDSDSDSDSD
                     SDSDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSESDSDSESDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDAGKHTPVKPMSATKDHHNKAKALPETGSENNGSNNATLFGGLFAALGS
                     LLLFGRRKKQNK"
     gene            complement(15727..16293)
                     /locus_tag="O0V33_001336"
     CDS             complement(15727..16293)
                     /locus_tag="O0V33_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCX8975975.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFVNNSPYEHLK"
     gene            complement(16313..17182)
                     /locus_tag="O0V33_001337"
     CDS             complement(16313..17182)
                     /locus_tag="O0V33_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCX8975976.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKDVMAIGDNLNDVSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            complement(17329..17799)
                     /locus_tag="O0V33_001338"
     CDS             complement(17329..17799)
                     /locus_tag="O0V33_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HCX8975977.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEDCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            17866..18483
                     /locus_tag="O0V33_001339"
     CDS             17866..18483
                     /locus_tag="O0V33_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCX8975978.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSSVEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREY
                     YESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE"
     gene            18476..19138
                     /locus_tag="O0V33_001340"
     CDS             18476..19138
                     /locus_tag="O0V33_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCX8975979.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMIDKIARIIQTYRQVD
                     TR"
     gene            complement(19254..19937)
                     /locus_tag="O0V33_001341"
     CDS             complement(19254..19937)
                     /locus_tag="O0V33_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCX8975980.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALI"
BASE COUNT         6047 a         3440 c         3010 g         7498 t
ORIGIN      
        1 ataaatatgg attttaacca tggtattctc tgttttaaca aattttataa tacggtatta
       61 atttctgatg gatcattgat tataaaatct ggatttgaat gaagtaatga ccttgcatca
      121 tgtgctcccc atgttactgc tgcagatggt acaccagcat gttgcgccat ctcaacatca
      181 aatgttgaat caccaatata aaccgtttgt tggctattta aattgtagcg ttgcacaatt
      241 gtgtgtatgc cttcaggatt tggtttatat gcacttactt gatcggatcc aacagcttca
      301 gtgattaaat gatttaaccc tatagccgat aaatttcttt ctaatacatc actcttctta
      361 ctagacacca cgaaaagttt tttcccttgg ttatacaaac ttgtaatggc ttcagttata
      421 cccgcaaatt cataaatata gtccttttca atagattgat atgtatgtct aaatgtatcg
      481 attaactttg ctaatgttgc ttcatctaat ggtcggtctg ctaattttaa aaatgattct
      541 tcaataggta ttcccatata atgcgatatg tcttgagctg atggttctgt taaaccacat
      601 gctttaaatg cactttgtgt tgctacttca ccacattttt tcgtgtctgc caacgtacca
      661 tcaaaatcaa aaatataatt ttcaaacttc aatattaaca cccctattcg tttattattg
      721 atattatact ataatttatt atttaaataa aaaaataatc gataatattt ctgcctaggc
      781 atcatattat cgactatctt tgttttattc taaattagca tgattttgtt gcatcgtttg
      841 ttctgaaaca ttcatttcag tcatcaatcc cattacaacg ctatctaaaa atagttgaga
      901 tgcttgttca aacaaacttc ctaacggttg tgcagaacca tgttcatcat attttgtacc
      961 tgcaggcaaa acgatgttcg tcccagctag attgcctatt gcagaatctt tatttgtagt
     1021 aattaatacg atgtcagcac ctactgattt tgctttatct gctaataatc ttaaatgttc
     1081 cgtggaacct gaaccagaga taattacaaa tacatcattc gacttaatcg caggtgtcgt
     1141 tgattctcca acaacatgtg cctgtttgcc gagctgattt aagcgcattg caaaactatt
     1201 cgccacgaat cctgaacgtc ctttgccagc tacaaatata tgttcagcat gtagtatttt
     1261 ggacgcaaac gttgaaaact catccgcttc aacatgtgac aaagtcatct ttaattcatc
     1321 tagaattaat tgatagtcac taaatttagc catattactt accttcgatt gcagcgcgac
     1381 attgttttgc agcttctact ggatcatctg catttgcgat tccgccacca acaataacaa
     1441 gatcaggact ttcagcgaca atttctttaa ttgtatctgg tttaattcca cctgctactg
     1501 caactttaga atttttaata acagatttaa cggttcttaa actttctaat ggtgattgcc
     1561 cttctgcttg taaatcataa ccagtgtgta ctgcaatata atcagcaccc atttcatcta
     1621 gttcttttgc acgtttttct aaatcttgaa cagcaatcat atcaactagt aattgtttat
     1681 tatttttatg agcttcttca atagctgctt taattgatgc atcttctgca acacctagta
     1741 ttgtaattac atccgcgcca aatttaattg cttggctaac ttcataatca gctgcatcca
     1801 taattttcat gtctgctaat acttttacat tactaatgtt gtctgccata tgtttaactg
     1861 ctggtaaacc ttcgttgtaa atgattggcg taccgatttc tacgatatct acataatctt
     1921 ttactttatt tgctaactca gccgcgtctt ctttgtttaa taaatcaatt gctaattgta
     1981 attccactaa aaatcatcct tccaatgctt tttatctttc tattggaacc tttcccgagg
     2041 catttcttaa ttaaacattg caatcaatcc atttcatcga catgaatttt cgcaacgttt
     2101 ttcgggcaag cttcgtcgtc taaatatatc tcaacattcg ggtgtttgtg aagtaatgtg
     2161 gctggaacat caacagaaat ttcctgattt aataaatgtg taatagcagc acgtttcttt
     2221 tcaccaaatg cgagtaaaat gatacgttta gcttgaagaa tattagcaag tcccatcgaa
     2281 atggcttgct ttggaacatc atcttcgttt tcaaaatatc gactattagc cttaatagta
     2341 ctttcagtca aatcaacgat atgagtaacg ctatcaaacg gcgtaccagg ttcattaaat
     2401 ccaatatgac cattttcacc aatacctaaa atttgaatat cacgttgacc ttgttgttct
     2461 aaaacgtcat tatatgttga cgcttccgca ttcatatcat aggcatctcc attcggaata
     2521 tgaatgttct ttctattaaa ataagggtat tgtttgaaaa gcatgtcatc catataatag
     2581 tgataacttt gcggatgtga tgcggttaaa cctacatatt cgtctaaatt aaacgtggat
     2641 acattgtcta cgtttaactg atttttattt aataacttaa caagttgctc atacaaatct
     2701 gtcattgtgc cgccagttgc taaaccaagt ttacagtgct gattaaatgc catctcttta
     2761 tataactcac atgcaacata gaatgatgct tgtttttttg aacctaagtt taatactttc
     2821 attgtttaca tgcctccaca aatttaatac attttaaaaa cttatgaatt cattcataca
     2881 ccattaatat agcatcattt ttaatgttta acacttttca aattccaaca atttttttga
     2941 tttttaaaag taaaaaacta atgctgtatg attgcgtaat cctgagtcat tcagcttcat
     3001 gaaaattaat ttataaataa ttgagcaatc gtatacaatt attgaatagt ttttagtaaa
     3061 atagaaagat aaacacattg atagtgtaag gagtgaacgt gttggaacca attaaagaac
     3121 aagaagttat agatttactt gcttcttttg aacataaacc tgtgtatctg catgttgaaa
     3181 cgacaaatgg ggcctatgcg aaccatttcg atcaacgtgt ttttaatgct ggtacttttt
     3241 taagaaacat acaagtaaca tatacacatg cacaactaaa aggtggtaat aaagaacctt
     3301 atcgcattgg tcttaaacta tcaaatggtg gttgggttta tgtacaaggt ctaacgcatt
     3361 ttgaaatcaa tgagcatgat gaatttttaa ttgcaggttt taattacgaa ggtcaactcg
     3421 ctgcagcact tcaaattagt gagcgaccat ttaatttata gaggaggcat caacatgact
     3481 gacgaaagac atgtattagt aatatttcca catcctgatg atgaaacctt ctcatctgca
     3541 ggcacattag caagttatat ccaaaaaggc acacctgtaa catatgcatg tttaacccta
     3601 ggacaaatgg gcagaaattt aggtaatccc ccatttgcaa cacgcgaatc attaccaagt
     3661 attcgtgagc gtgaactaga agaagcttgt aaagttattg gtattactga tttaagaaaa
     3721 atgggtttac gtgacaaaac tgtcgaattt gaaccctatg aacatataga tggtatgatt
     3781 aaatctttaa tcgatgatac aaatccatca ttgattattt cattttatcc tggatatgca
     3841 gtacatcctg accacgaagc aacagcagac gctgtaattc gaacggttga acgcatacct
     3901 aaagaagaac gccctcgttt aactttagtt gcgttcagta atgatgcaac cgaagcactt
     3961 ggagaacctg atatccaaaa tgatattaca gattttaaag aacttaaaat taaggcattt
     4021 gaagcacatg cttctcaaac aggtccattt ttaaaacaat tagcaagtcc tgaaatcgat
     4081 ggtaaacaac acagcttttt aacagtcgaa ccatattgga catatcactt taaatcttaa
     4141 cggaggaaaa aaatgactga atttgattta tcgacacgag aaggtcgttg gaaacacttt
     4201 ggttccgtag atcctattga aggcacgaaa cctactacta agaacgaaat gacagattta
     4261 caaagtacac ataaagattt cttatttgaa atagaagaag ttggtattaa aaacttagtg
     4321 taccctgtac ttgttgacca gtatcaaaca gctggcacat ttagtttttc aacaagttta
     4381 acaaaagacg aaaaaggtat taatatgagc cgtattattg aaagtgttga aaagcactat
     4441 gataatggta ttgaacttga atttaataca ctttatcagg tgttatgcac tttacaaacg
     4501 aatatgaaac aaaatgcggc aggcgttgat gtttctggga aatggttctt tgatcgctat
     4561 agtccaacca caaatattaa agcagttggt aatgccgatg tcacttatgg attagcaatt
     4621 gacggtgata aagttacacg taaagaactt actattgaag caactgtcac aacgctatgc
     4681 ccttgttcaa aagaaataag tgaatattca gcgcacaacc aacgtggtgt tgtcactgtt
     4741 aaaacatata ttaataaaga tcaagacata gttgatgact ataaaaacaa aattttagat
     4801 gcaatggaag ctaatgcaag ttcaattcta taccctattt taaaacgtcc tgatgaaaaa
     4861 cgtgtgacag agcgtgcata tgaaaatcca cgctttgtcg aagacttaat tcgtttaatt
     4921 gccgcggact tagttgaatt tgattggtta gaaggtttcg atatcgaatg tcgtaacgaa
     4981 gagtctatcc atcaacatga tgctttcgct aaattaaagt atcgcaaata atatcatatg
     5041 attaattcaa acgcgtaaca gtagttgcgc gtttttatta tacctcctag agttgtatat
     5101 ctcttttata aatcttctca tttgatgtgc ctgagtcata aatcaatatc ctaagctcta
     5161 caatgatata ttggtagtat ttgactgaat aactactacc attataaata taaaaatttg
     5221 ttgttttata aataaacaca tcttctagtt aaaagaagta attaaatatt atgtgatata
     5281 attataaaaa atagatataa gtggtgttgt aaatgaatta ttttgtaggt aatagtttag
     5341 gtgttaattt aactgggata gaaaaagcaa ttattaatcg tttaaattta tttaaagaaa
     5401 tgggaagacc agtacaatgt gtatttctgt catggaatag atatttatat agaaatgccc
     5461 aaaattatat tactagctct gactatatca atatgtatga tttcttccaa gaagcaacat
     5521 atttagaacg caatgaacca tttgactggt tgtcatattg gactgatgaa tgtcattata
     5581 ctttaaaaca tgttgaaaat agtcatgact ttagaatata tgatcaagag cgtttcttaa
     5641 tgtatgccca ttttcaagat cctaaatatc gtatattaga ttatgtgaat cattttgata
     5701 gtcaaagacg aaaagtaaaa cgtgattttt atgatgtgcg tggcttttta agttgtagta
     5761 ggattcttgt tgataaacaa caaacattat gtgaattttt ctataatcca gaaggtgaca
     5821 caaaattaga aaagtatttt tcttacaaag atggcaaacc agaagtacaa aaaattattg
     5881 tttactatgc taacaaacaa tatttcttta ataatgaaac ggaattaggc gcatttttca
     5941 ttaaacaact atatcaacat ggggatttat ttttcagtga ccgtaatgtg tatacagcac
     6001 ctattttcaa cttaacacct gagtcaatcc cagtagtagc tgtgcttcac agtacacaca
     6061 ttaaaaatat tgatgcatta gattcatcgc catttaaaaa tgtctataaa gcgatgtttg
     6121 aaaatttatc tcgatatcgt gccattatcg tttctactga gcagcaaaaa ttagatgttg
     6181 aaaaacgtat taaccatact attccagttg ttaatattcc tgtcggctat agtgaaacaa
     6241 ttgatacacc agtccaaaca ttagatcagc acagtgttaa attaatttcc gttgctcgtt
     6301 attcacctga aaaacaatta catcaacaaa ttgaattagt taaacgctta gtcccttatg
     6361 taccgaaagt agaaattcat atgtatggtt ttggttctga aagtaaaaaa ttaaatgaat
     6421 tgatacaaaa atacggattg gaaaatcatg tttatttacg tggcttttta agcaatctag
     6481 accaagaata cagcgatgct tacttaagtt taataacgag taacatggaa ggtttctcgt
     6541 tagcgctact tgaatcttta gcacatggtg ttccagttat tagttatgat attaaatatg
     6601 gtcctggtga attaattact tcagatttca atggttattt aattactaaa aatgatgaag
     6661 atgccctatt tgataaagta aaatatgtga ttgatcatcc tgaagtacaa caacgactgt
     6721 caaaaggtag tttggcaaaa gcgcaacaat atagtaaagc gtcgttaatt aaacaatggg
     6781 accaatttgt tcgtctaata taaatgtact ttacctttat ttttataaaa aaaaagcatt
     6841 ttcctatgtc atttaatcaa acttgtcagt tcttatgaac gtggattgtt aaatagatga
     6901 ttttaggaaa atgctttttt cttttgactt agtttaaaat tttttgccac tttgtactga
     6961 tagtagttgc attgtactgt tgtgcagatt ctatgctatt aattgaaaat tgttgcatca
     7021 tttggctatt atttaataat tggtcaacct tttcaaccat ttgattgatg tcacctggag
     7081 gtactaaata gccattaaat ccatcttgaa tcagttctga tggcccataa tctacatcat
     7141 aactgatcac tggtgtacct actgaaagcg actctaaaat tgctaagcca aaaccttcca
     7201 ttttacttgt cgataacatc agttctgctt tagcaatctc ttcattaata tgcgtcttaa
     7261 aaccatgaaa tttaacatgt tccgataaat gataaccttc tacaagttgt cgatattctg
     7321 acaaaccatt tccatgtcca taaatattca attgaatttt gggatgtttg gttactaatt
     7381 gcttgatcac ttcaatttga tgtgtaattt gtttgttttc aacgaggcga gcaattgata
     7441 tgatatgatt tttatccttt tgattgatgt caaattgata ctttaagttt gccacgtagc
     7501 caaccggaat attgataact ggtattttat tttcaatata ttgtgaaata tcttggcatt
     7561 gcttttctgt tgatacaaca atcgctttat aacgtgttaa attattaaac actgttttat
     7621 aaaaattttt tatatcatta ccggtgccgg ataaatgtgt actgtggagc acaacaataa
     7681 ctggaatact ttgatttaat cccgcaataa catttcctaa ttcatgagga cggtctaata
     7741 tgatttgatc attattttta cataattgat ggagaaaata ttgaactaat tcatcttctg
     7801 tatcaaaaaa ttgttgatgc tggtcttcat ttaagataac ctttgtgagc gtgtttttcc
     7861 cttttatatc gtcgaaatat ttttggatga cgatttcccc attaggtgta tagtaatttt
     7921 cgagtacaat ccgttgtcct tcacctaaaa ttcgagaaca acttaaaaag cctcttccat
     7981 catacaattc acgttttact tttcttcttt tatgatcaaa ataattcaca tagtttaatt
     8041 gatgatactg tttatctaaa aaatgagcat acattataaa ttgctcttca tcatatattc
     8101 tgacatcatt tgaattttcc acaaatttca acgtgtacct acatgacttt tcccaatact
     8161 gtatccagtt aacttgcttt gtctttttat aattgattgc tttttgaaaa tagtcataca
     8221 ttgtaaacag atcattttca atctgatgtt gcttcgcata tgtgtatgaa taaggattcc
     8281 atttaacata tacacatttt gaagatatgc cgtgttgttt gaacaacttc agtctattta
     8341 tttgtgcttt ttctacacct gtaattttac tttctaaaat tgttcctaaa atgtaattca
     8401 tattatcgcc tcatataagt tttattccgt atctttattg tttattttat atgaaaaata
     8461 catctattgc atgtgtaatc ataaaaaaac caggccacaa ggacctgggt caaattgtat
     8521 tatttgtttt gttttttgcg acgaccgaat aacaataatg aacctaatgc tgcgaataat
     8581 ccaccaaata acgttgcgtt atttgagccg ttattttcac tacctgtttc tggtaatgct
     8641 ttggctttat tgtgatggtc tttagtagca ctcattggtt tcacaggtgt atgttttcct
     8701 gcatctgaat ctgagtcgct atccgaatct gagtcgctat ctgaatctga atcgctatct
     8761 gagtctgagt cactgtctga atctgagtcg ctatctgagt ctgagtcact gtctgagtct
     8821 gagtcgctat ctgaatctga gtcactgtct gagtctgagt cgctgtctga gtctgaatca
     8881 ctgtctgaat ctgagtcgct gtctgagtct gaatcactgt ctgaatctga atcgctgtct
     8941 gaatctgagt ctgagtcact gtctgatgta tcttcttcga agtatccatt atcaagtgtg
     9001 aaatcatcat gatccgtaat tgttacgtca acttcgccac catctgcatc tttatcatct
     9061 tcagttgtat ttgtacctgt ttgtgttaag ccagcaggct tttcaaaaat aactttgtat
     9121 ttaccgctat ctaaattatc gaaatgatat ttaccatttt catctgtttt agttgttcca
     9181 attacttcgc ctttttcgtt ttgcaatgta actgtcacat ctttaatacc cttttcagtt
     9241 gaatcttgtt tgccgtcttt attactgtcg taccaaacat aatcacctaa actatatttt
     9301 ggtgttttat agaaaccact gtctaatgtc caattatctg catctttaat aacacctgtt
     9361 gttgttaaac cattagaatc tttatcttca gctgtagtgt ttttaaccgt tggcgtgtaa
     9421 cctgctggtg tttcaaattc tactgtatag ttaccattat ctaaatctgt aaatttatat
     9481 ttacccgagg catcagtagt agttgtacca ataacttttc cagtactatc ttttaatgta
     9541 accttaacat ctttgatacc aggttcatta gcatcttgga taccatcttt attcgtatct
     9601 tcccatacat agtcaccaag attatattta ggttctttat aaaaaccagt gtctaaagac
     9661 atatcatctt taccattaat tttaacagtt acagaactac catttgagtc tttttcacca
     9721 tcagttgttc cattttcttt tgttggaaga tatccagctg gcgtttcgaa tttaacagta
     9781 taagtttctc cgtctttcaa accaccgaat tcataatgac cgttagcatc tgttcttact
     9841 gattttgtag taccgtccgg gtaagttaat gtaactaaaa cgtttgccat tggcttttct
     9901 tttgaatctg taccttgaac accgtcttta tcaacgtctt cccatacata gtcaccaatt
     9961 ttgtataact tttcttcagg tttaacagta ccgtcaccac caccagagtc agtagttgat
    10021 aaaatagtat tagtgtaacc tgcatatgat gaataattat atttatttgt tgtagtcatt
    10081 cttacaccct gttgaactgc taagtcatcc tcagcacccg gtgtatattt actaacaact
    10141 ttaactatat atgcagaatc aatgtcacca aaattaatat tagccatgtt agtaccataa
    10201 tttttattaa tagttactga attggtaaca tcttcatatt gactataatc ataaatttta
    10261 ttactttgag gtaattgttg acccgatgca actttataga ctttaatttc cgtattacta
    10321 tcaattatag tactaccgtc tccagtataa taatcaccat tatctgctac accaccactt
    10381 tttatagtaa catttgtgtt tttagcattt aattttaatg gattcacata aatagtctgt
    10441 tctacctcat gcttagtagt atctaaatgg ctaaatatag attgaatatt tgactcatct
    10501 tttacaatag gtttttgata ttcaacttta acatttttac tagtttcttt atctgctgta
    10561 gcaaatgtta aatttaaatt tgtttcattt gggacttctt tcttatcaat atatgagttt
    10621 agttctaatt ttgcattgac attctcatat ctatcaacat aatcagtaaa tttgtaagta
    10681 attgttttcg tatttaaatc aaatgttccc ttcgctatta cttcacctga tggatctgta
    10741 atatcaactg gtgtgaaatc atccgtaata tctgaaggaa ttgtatgctt atcatacttt
    10801 actgtcattg tatccccagc ttttacagca ttatcaattt taaaatcgga tgtataaata
    10861 atatgttcac cgtcatgtgc ttgaataaca ccatcatctt taataccttc agtaatcatc
    10921 tgtttcgtca ctgtaattaa atcattaaca ttgtttgaag taactgctgc tggttgtgca
    10981 actgcaaagc gtatttttgc gtttacacgt tttggtgcaa cacttgttgg agctgtagca
    11041 actggtttag ttgaacgatc tgtattgcta tcatttctaa ctaattcttt taatttctca
    11101 ggattatttt taagttcttc ttttgacaca tttactggtt cttttggatt agttgcatct
    11161 gtaacttgat tgtctacttt tgaaggcgtt ggttgcggtt gtgaattttc aatatttgta
    11221 gattcttgag gtgtagttgg tgttgtttga atttcacttg tagatggttt tgtagttaca
    11281 tcgttatttg tattatttgg cgctttcttc tcttctaaaa taacagatgt atcttctgtc
    11341 gcagttttat cagttgaagg tttaacattt tctttttcaa tgttttgagg cttagtttca
    11401 gttgaagttg tagcattatt ttgctgttgt tgagtactag atgtagttgg tgcttcttta
    11461 gcttcttggt gtgaatcagt atttgtttct ttcttaattg gatttgttaa atcatttttt
    11521 tgtgtcgaat tattttcagt ttctgacact acttctttat tatcactagc cgatgcctca
    11581 tcttgcttag cattttctgt actagtgttt tcagcagctt tagcttcttg gttccctaga
    11641 ccaaaaatca atgtcgtacc tactaaaatc gatgcagttc ctacagtata ctttctaatc
    11701 gagaatttgt ttaagcgatt cgaaatcata cccttttttg ttattgcctt tttgttatcc
    11761 ctgttaatca tataaatctc ctttaaaata tcaatatttg caaaaaacta atatttttac
    11821 atataaaaat ttgtattccc atcgtaatac atttttagat aaagcacaat actttaaata
    11881 aatttattta ttttcaactg ttattttaca ctttggtttg aataacttaa ttttattgat
    11941 ttttaattat ctatttactt tttatactaa ttgttttaaa tggtttgaaa ctgtgtttaa
    12001 ttgtttttct tatttgtctg tacttttaat ataaagatga attaaattta tgttgatgca
    12061 tgttatatct tttaaaatat tgcactattt aatctcaaac ataagataca tttcataaat
    12121 tttgaaataa aaaaccaggc ctcacggacc tggtttggtt aagcaaatta cttattctgt
    12181 tttttacgac gaccaaataa caataatgaa cctaatgctg cgaataatcc gccaaataac
    12241 gttgcgttat ttgagccgtt attttcacta cctgtttctg gtaatgcttt tgctttattg
    12301 tgatggtctt tagtagcact catcggtttc acaggtgtat gttttcctgc atctgaatct
    12361 gaatcgctat ctgagtctga gtcactgtct gaatctgaat cgctatctga gtctgagtcg
    12421 ctatctgaat ctgagtcgct atctgaatct gagtcactgt ctgagtctga gtcactgtct
    12481 gaatctgaat cgctatctga gtctgaatca ctgtctgagt cactgtctga gtctgaatct
    12541 gagtcgctat ctgagtctga gtcgctatcc gagtctgaat cgctatctga gtctgaatcg
    12601 ctttctgaat ctgagtcgct ttctgaatct gaatcactat ctgagtctga atcgctatct
    12661 gaatctgagt cactgtctga gtctgagtcg ctttctgaat ctgaatcgct atctgagtct
    12721 gagtcactgt ctgagtctga gtcactgtct gagtctgaat cactgtctga gtctgattca
    12781 ctatctgagt ctgagtcgct ttctgaatcg gaatcactgt ctgagtctga gtcgctgtct
    12841 gatgtatctt cttcgaagta tccgttatca agtgtgaaat catcatgatc cgtaattgtt
    12901 acgtcaactt cgccaccatc tgcatcttta tcatcttcag ttgtatttgt acctgtttgt
    12961 gttaagccag caggcttttc aaaaataact ttatatttac cgctatctaa attatcgaaa
    13021 cgatatttac cattttcatc tgttttagtt gttccaatta cttcgccttt ttcatttaat
    13081 aaagtaactt taacatcttt gatacctttt tcagttgaat cttgtttgcc atctttatta
    13141 ctgtcgtacc aaacataatc acctaaactg tattttggtg ttttatagaa accactgtct
    13201 aatgtcatat tatctgcatc tttaatgaca cctgttgttg ttaaaccatt agaatctttt
    13261 tcagtatcat ttccagtagt tactgaagtt ggtgtgtaac ctgatggtgt ctcgaattca
    13321 actttataag ttccattgtt taaatcagtg aattgatatt taccattttc atctgttgtt
    13381 actgttttta aaactttgtc gttttcatct tttaacgtaa ctgttacacc tgaaatgccc
    13441 ttttcatctt tatcttgaac accgttttta tttgtatctt cccatacata gtcacctaag
    13501 ttgtaagtcg gtttgtagaa accagagtca atagtatcgt tatctttatc tttaatgaca
    13561 cctgttgttg atgtaccatt tgaatctata ccttcatcag ttcctgaacc tacttgagtt
    13621 ggtgtgtaac ctgatggtgt ttcaaattca actttataag ttccattttc taatccagta
    13681 aattgatatt taccatcttt atctgtttta gttgtttgta aaacttcacc gttttcattt
    13741 ttcaatgtaa ctgttacgcc tgaaatacct ttttcagatg aatcctgctt accatcttta
    13801 tttgtatctt cccatacata gtcacctaga ttatatttct tttggtcgcc attagcagtt
    13861 gatgagccgt ttacacttga ataactgttc gaccaactgt atttagtttt gtctgtatct
    13921 aatgtataat caatttttcc attatctgtt gatgtattat ctggataagc aacttgttga
    13981 ataatatatt gtttattact atttgtttga ccattcatta aatcaactgt tgcagtctta
    14041 ttatcattgc tgtatgtgat tttgaattta tcagtaacat ctataagttt tgaagtatca
    14101 ggtgtaaagc tatcaacaaa ttgattttga tctgtcactt cataaatttt aaagtttttt
    14161 gcatctggat taaatttata acctgttaaa gtactaacaa atgtttcttt tgtgtatgta
    14221 tttttaggtt gatttacata aacagtcata tttcgtgata aatcttcatt attgatatag
    14281 tttgtacttg aaataagcgg ttgtgctttt ttattaccat aatcgacgat gatttcttcg
    14341 ctatacgcat cattacctaa agttacttcc attttataag cagttttatc agttgttgca
    14401 ttttcacgtt tcgcaaatgc aacttgttca aagctaccac taacatttgt atattgatcc
    14461 acatagttcg taaaagtata tgttgttgtg tttgttgtac tatcataaat accttttgca
    14521 ataatattac cttgggcatt atataaattt tgagtttgtg aaggtaatct tacagaaccc
    14581 ggacggaaat actgtccata tttaaatgta aatgtatcgc cctctttaac agaatcatcg
    14641 attgtgtaat tcgcttttaa ttttaaaaca tcactagaag tcgcccaaaa ttcagtttta
    14701 cctgttgtag aattgacatg tcctttatca atagcaatat caatatttga aaaatgaact
    14761 ttatcattta catttgtacc ttgttgtggt gctgctactg tgttcacagc catacggttt
    14821 aatgttcttg gtttaatagt cgttgtttta ggagtagctg aaacatcatt agcctgcgtt
    14881 gaaatatttt gagccgctaa attatcttta tttgctgtcg catcgttatt ttttttagtt
    14941 ggttgtgtag aagttgtatt ttgaggttct tcagtagttt ctttaactga agtattatca
    15001 gactcattta cttttggttg gtttgacgtt gtactttgtt caacattatt ttgttgacga
    15061 ctatcaactt tttcagttgt tttattctca cttggagctg tcgcttcatt ttttgattga
    15121 tttaattctc cattcgtatg ttctgccgct ttagcttcat gaccacttaa cccaaaaatc
    15181 aatgttgtcc ccactaaaat tgaagcagta cctacagaat actttcttat cgaaaatttg
    15241 tttaatcgat ttggtatcat gccttttcta tttgttgctg tctttttatt attcatttaa
    15301 taatactcct ttaaaatatc aaaatttgat aaatataata ttttgcatat aaaaatttct
    15361 gcctttctat cttaatacac ttttcgataa agtacaatgt tttaattttt ttcttttatt
    15421 ttacaatgtt atttatagta aatattaata tgcatttcag attaagaatt gcaattttta
    15481 atactttttt tcttccattg ttttaaatta tttatctata tttttaaact ttccttttaa
    15541 tctaagcttg ttttcaattc gctattacat cttattttat ttaataacat tgctcacaat
    15601 agtaaactca aaggcttact attggtgcat tagaacaaaa aaataggcat gtgacttaaa
    15661 agcattctca atgaaatacc ttaagctaca tacctatttt taatgtatat ttacatattt
    15721 tttaatttat tttaaatgtt catatgggct attattcaca aaagatacaa tttgatcgac
    15781 aaataatctt gctcgtaatt ggaattcact atcatttaag taatatgaac catcttcatt
    15841 tttagcaaaa tcagaatcat gtgttgctat ttgagttggt actgcaatgc ctagtaaact
    15901 tctgacgatt actcttaaat gtgacaatgg ctctgaacta acaattccac cactatttcc
    15961 tattaagcct acaggtttca ttttaaaata atccatattt agatgatcta atgcattttt
    16021 caatattcca gaatatgaac catgatagtt tggcgttcct aatattaaaa agtccgccgc
    16081 cattgctttc tcttttaaat ctttcatatt ttgtttgatt tcatcaatag acggtgttgt
    16141 tcctgaaaaa tctaattgat ttaacggttt ttctgctaaa tcgaatattt ccgcttcaat
    16201 atcatgtgtt ttaaaatgct cagttaagta tcttgctagt gcacttgtat gtgaattcac
    16261 ttgtgcactg ccaataataa ttaatccttt catatttgac ccctcttttt tattaaactt
    16321 gttgttcacg taataatttc ataatagctt ttccaacacc actattttca tttgtatctg
    16381 tgacatattt cgctattttt ttaacttctt cagcaccatt ttccatcgca actggatagc
    16441 caactttctc taacattgat acgtcattta aattgtcacc tattgccatg acatctttca
    16501 tttcaatccc taatctttcg gcaattgttt ctagcgcaat gcctttttgt gcatctgaat
    16561 gcgttatttc tatatttcct ctcgaagatg atgatatagc taaattcgga gattcagcta
    16621 aaattttact agctttgtca attttttcta aatttccatc aaatgctaat attttcataa
    16681 ttaattcacc aggtatgttt tcaatagcat cataattatc aacaactttc aacgtaccat
    16741 tatctatgcg tctttgaata ccatttttaa tacgctcaac gtttgcgtgt tgacctgcac
    16801 gctcagcaat atctatgtaa atgtctaaat ctctttgtgg atcttcagta taaatcgcac
    16861 gactcgtgta tacttgataa taaatacctg catcttttaa aacatttgta attttgtgta
    16921 ctaacgattt attaaggtgt gaagtgctca ttacattgaa agtttcatca cgtacttcag
    16981 caccattcaa acaaatatat ggtactgtta aatctgtgtc agcaactggt gcttgtgctt
    17041 cataaaatgc tcgacctgtc gcgataacaa ccgttatccc ttgttcttga gcgtatttaa
    17101 tcgcatcaat attaggttga gaaatttcat gtgctgcatt aagtagcgtg ccatccatat
    17161 cagtggctat tagttttatc attatgttac ctcgtttcgt aaatttaaaa tcttgttctt
    17221 acataagaat atatactcag tgcacatact tttctattaa catttatatt gtcattaatt
    17281 tatcatataa tgtaattcta acaaatttta attagtatgt actatcgtct aattggtgga
    17341 tttcttattg gctcttaagt ttttaaaaaa tgttgttaat aatgtgctac atgcttcttt
    17401 aagtacacct ttatcaacaa ttgcacgatg attaaaatta gattgttgca ataaattcat
    17461 taaactgcca ctacaaccac ctttaggatc atctgcgcca tagacgactc ttggaatgcg
    17521 actcattaca attgttcctg cgcacatgac acatggttct aaggttacat ataatgtgca
    17581 atcttctaaa cgccaactac ctaacacttt ggctgcacgt tcaattgcaa tatgttcagc
    17641 atgcgccgtt ggttgttgta gtgtttctct taaattatgt gctctagcga taacttcatc
    17701 atctttagtg atgatagcac ctataggtac ttcgcctagt tgagctgcct ttttagcttc
    17761 ttcaatcgct aatgtcataa aatatatatc atttgtcatt tatgtccaga tacctcactt
    17821 atggtacaat actcaatgtc tattttaaca tttggagatg tgaatatgaa caaacctttt
    17881 atagcaattg aaggtcctat tggcgtaggt aaatcttcac ttgcacacaa attaagtcaa
    17941 actttagatt tttatgaaga aaaagaaatc atcacagaaa atccattttt atcagacttt
    18001 tatgaagata tctctaaatg gagttttcaa actgaaatgt tctttttatg caatagatat
    18061 aagcaatttc aagatgtaac acaactaaat caaggtgtag ttagtgatta tcatatacat
    18121 aaaaataaga tatttgctaa aaatactttg agttctgttg aatttcagaa attcagtaaa
    18181 atttatgata ttttaactga agatatgatt atgccgaata tgattatctt tttagatgca
    18241 gaccttgatg tgttaaaatc tagaattgct aaacgtaacc gtagttttga gcatcaaata
    18301 gaagatgaat atctgttaaa gttaaaaaaa gattatcgtg agtattatga gtccttacaa
    18361 agtaatggtt caaatgtagt tttaatcgat acaacttcta ttgattttct aaaaaatgaa
    18421 caagattacg aagatatatt acatattata ttacctatga taggagatat taccaatgaa
    18481 taattacggt attccacaaa atgccattat aaccattgca ggtacagttg gtgttgggaa
    18541 atcaacacta acgcaagcac ttgcagataa attaaacttt aaaacgtctt ttgaaaatgt
    18601 cgaacataat ccatatttag ataaatttta cagcgatttt gaacgttgga gtttccattt
    18661 acaaatttat ttcttagctg aacgctttaa agaacaaaag cgtatgtttg aatatggtgg
    18721 tggctttgtc caagatcgat caatttatga agatgttgat atttttgcaa aaatgcatga
    18781 agaagaaggc acaatgagta aagaggattt caaaacatat tcagacttat ttaatgccat
    18841 ggtcatgaca ccttattttc ctaaacctga tgtaatgatt tatttagaat gtaactatga
    18901 tgaggtcatt gatcgtatta ttgaacgcgg tcgcgaaatg gaaattaata cagaccctga
    18961 atactggaaa aagctattta aacgctatga cgattggatt aatagcttta atgcatgtcc
    19021 tgtggtacgt atcaatatta atgaatatga tatccataag gaccccgaat ctttaaatcc
    19081 tatgatagac aaaattgctc gaattattca aacatatcga caagtagata cacgataaaa
    19141 gactaaagac atagcgtata tgtttatatt caatgtatat tccatagata tcatcgatta
    19201 cattatcaat tctattggtt acattgattc acatatacac tatgtcttac tttttaaatt
    19261 aaagcttcta aaatatctgc cgcactattt aaaataatat cagcttcatg taattcttct
    19321 tttgttgcaa tacctgttaa tacacctatt gccataccta aatttgcatt actagcggtt
    19381 ttcatatcat tagcagtgtc tcctactata gctactttct gaggatctac attatattgc
    19441 tcaaataaag gcgataatac tttaggattt ggcttctcat aggcatccgc ttcggtagaa
    19501 atgatcaaat cgaacaacga ggtagcattg gtatgtgcta aaaattgttc tacacctttt
    19561 ttagtatcac tcgtaacaat acctagttga tagccttttg ctttcaaatc gataagtgct
    19621 tctttaacac cttctaccca attaatttca ggaatacgtt catctaccaa cttttgactt
    19681 gttgacttgg accagtcggt tgtatcttgt cccgtcacat cattaaatgc ctgaataatt
    19741 tgttgtaaag atcctgaacc catcactgat tttggatcaa tagattcttt aatgacaccg
    19801 agttgtctta aagcagcttc tttattatgt actggaaaag tctcaagcaa tgattgtaca
    19861 aatcgtaccc ctattttttc ccaacttcta tcaaattcaa ttaacgtacc atctttatca
    19921 aataatatcc attccattaa tatcaatact cctatttatt tatttcgtat tatgctgatt
    19981 ctatgatgtt cgtta
//
LOCUS       DAKSQE010000045        19465 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.045, whole genome shotgun sequence.
ACCESSION   DAKSQE010000045 DAKSQE010000000
VERSION     DAKSQE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19465
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.045"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            65..877
                     /gene="tagG"
                     /locus_tag="O0V33_001342"
     CDS             65..877
                     /gene="tagG"
                     /locus_tag="O0V33_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HCX8975981.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEIMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
     gene            976..2079
                     /locus_tag="O0V33_001343"
     CDS             976..2079
                     /locus_tag="O0V33_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCX8975982.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSLYQKTVLTTPKQAPYLSELSDDVNVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKAMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLALYVPT
                     YREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEHSDIDDVSSIDTSILMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDKVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            2076..3137
                     /locus_tag="O0V33_001344"
     CDS             2076..3137
                     /locus_tag="O0V33_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCX8975983.1"
                     /translation="MRLTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLQRELKHISLIQLQNASIATCINKGLMEIKMTDPHDSDAFMVINPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDVTLANTYNHEILVKAMQKATDIHLVSQMIVGDNDIDTHATSNNE
                     DFKRYITEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFNGENWDREIYELWRQTLIQVGINRPTYKRF
                     LIQLKGRKFAHRTKSMLKR"
     gene            3200..3598
                     /gene="tagD"
                     /locus_tag="O0V33_001345"
     CDS             3200..3598
                     /gene="tagD"
                     /locus_tag="O0V33_001345"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HCX8975984.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(3715..5010)
                     /gene="pbp4"
                     /locus_tag="O0V33_001346"
     CDS             complement(3715..5010)
                     /gene="pbp4"
                     /locus_tag="O0V33_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HCX8975985.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQAANSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRTFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWEEHPLFTIIGGACLVAGLALIVHMIINRLFRKRK"
     gene            5431..7158
                     /locus_tag="O0V33_001347"
     CDS             5431..7158
                     /locus_tag="O0V33_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8975986.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYDIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            7517..8746
                     /locus_tag="O0V33_001348"
     CDS             7517..8746
                     /locus_tag="O0V33_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCX8975987.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GLIGGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            9001..9093
                     /locus_tag="O0V33_001349"
     CDS             9001..9093
                     /locus_tag="O0V33_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975988.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
     gene            9271..10104
                     /locus_tag="O0V33_001350"
     CDS             9271..10104
                     /locus_tag="O0V33_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCX8975989.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAALIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
     gene            10386..11183
                     /locus_tag="O0V33_001351"
     CDS             10386..11183
                     /locus_tag="O0V33_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8975990.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINGLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            11219..12223
                     /locus_tag="O0V33_001352"
     CDS             11219..12223
                     /locus_tag="O0V33_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCX8975991.1"
                     /translation="MTNRENPTPLKFLSYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGIALAVSGAIIQGVTRNGLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLISIALIIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADMIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            12220..13236
                     /locus_tag="O0V33_001353"
     CDS             12220..13236
                     /locus_tag="O0V33_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCX8975992.1"
                     /translation="MISSNNKRRQWIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDELILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGITAALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIILQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            13470..14438
                     /gene="dhaK"
                     /locus_tag="O0V33_001354"
     CDS             13470..14438
                     /gene="dhaK"
                     /locus_tag="O0V33_001354"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCX8975993.1"
                     /translation="MMKKLINKKETFLTDMIEGMLIAHPELDLIANTVIVKKAKKEQG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPIPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVETVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLA
                     EKGHSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVGTLLDELYKEVTANEVIVMVNGMGGTPLSELNIVTKYIQQNLAAR
                     TVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            14480..15064
                     /gene="dhaL"
                     /locus_tag="O0V33_001355"
     CDS             14480..15064
                     /gene="dhaL"
                     /locus_tag="O0V33_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCX8975994.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVAKDDMDNQDFIKL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLKNGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            15057..15419
                     /locus_tag="O0V33_001356"
     CDS             15057..15419
                     /locus_tag="O0V33_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCX8975995.1"
                     /translation="MPKIILVSHSKEIANGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEALTKLDDDALCFYDIGSSEMNLDMAIEMYDGNHHVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
     gene            15535..16032
                     /locus_tag="O0V33_001357"
     CDS             15535..16032
                     /locus_tag="O0V33_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975996.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
     gene            16488..17555
                     /locus_tag="O0V33_001358"
     CDS             16488..17555
                     /locus_tag="O0V33_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975997.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYVTLLLDFNKIVRALGVVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKKYHIFIVIMMIAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
     gene            17706..18749
                     /locus_tag="O0V33_001359"
     CDS             17706..18749
                     /locus_tag="O0V33_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCX8975998.1"
                     /translation="MNKDNKWTMITALFIAVISVLLAFHLKQHYDQTTNENHANKDKI
                     NIKNKNVRIYQNLTYNRVYPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVVIGG
                     DSAGAQLASQFTAIQTNNQLRKAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELKVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQI
                     ENPSNELPLNPLN"
     gene            19077..>19465
                     /locus_tag="O0V33_001360"
     CDS             19077..>19465
                     /locus_tag="O0V33_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8975999.1"
                     /translation="MTRSALKPFKNKRVMVTGRIHRVLFKNYLDRHSTFKPNVRILLK
                     DVIVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGNLVTEEAYIQNNQYQDKIYEKLPD"
BASE COUNT         6703 a         2701 c         3586 g         6475 t
ORIGIN      
        1 ttattataat actatttagt taagtagaga aatagttaaa catttgaaag tgtggtttaa
       61 tggaatgtca gcaataggaa cagtttttaa agaacatgta aagaactttt atttaattca
      121 aagactggct cagtttcaag ttaaaattat caatcatagt aactatttag gtgtggcatg
      181 ggaattaatt aaccctgtta tgcaaattat ggtttactgg atggtttttg gattaggaat
      241 aagaagtaat gcaccaattc atggtgtacc ttttgtttat tggttattgg ttggtatcag
      301 tatgtggttc ttcatcaacc aaggtatttt agaaggtact aaagcaatta cacaaaagtt
      361 taatcaagta tcgaaaatga acttcccgtt atcgataata ccgacatata ttgtgacaag
      421 tagattttat ggacatttag gcttactttt acttgtgata attgcatgta tgtttacggg
      481 tatttatcca tcaatacata tcattcaatt attgatatat gtaccgtttt gttttttctt
      541 aactgcctcg gtgacgttat taacatcaac actcggtgtg ttagttagag atacacaaat
      601 gttaatgcaa gcaatattaa gaatattatt ttacttttca ccaattttgt ggctaccaaa
      661 gaaccatggt atcagtggtt taattcatga aataatgaaa tataatccag tttactttat
      721 tgctgaatca taccgtgcag caattttata tcacgaatgg tatttcatgg atcattggaa
      781 attaatgtta tacaatttcg gtattgttgc cattttcttt gcaattggtg cgtacttaca
      841 catgaaatat agagatcaat ttgcagactt cttgtaatat atttatatga cgaaaccccg
      901 ctaaccatta ataaatggaa gtggggttca tttttgttta taatttaagt aaataacata
      961 ttaagttggt gtattatgaa cgttttaata aagaaatttt atcatttggt agttcgaata
     1021 ctttctaaaa tgattacgcc tcaagtgatt gataaaccgc atatcgtatt tatgatgact
     1081 tttccagaag atattaagcc tatcatcaaa gcattaaata attcgttgta tcagaaaacg
     1141 gttttaacaa caccaaaaca agcgccttat ttatctgaac ttagcgacga tgttaatgtg
     1201 atagaaatga ctaatcgaac actggtaaaa caaattaagg ctttgaaaag cgcgcagatg
     1261 attattatcg ataattatta cctattacta ggtggatata ataagacttc taatcaacac
     1321 attgttcaaa cgtggcatgc aagtggtgcg ttaaaaaact ttggcttaac agatcatcaa
     1381 gtcgatgtgt ctgacaaggc aatggttcag cagtaccgta aagtttatca agcgacagat
     1441 ttttacttag tgggttgtga acaaatgtca caatgtttta aacagtcttt aggtgcaaca
     1501 gaagagcaaa tgttgtattt tgggcttccg agaattaata aatattacac agctgataga
     1561 gcaacggtta aggcagagtt aaaggataaa tatggaatta caaataagtt ggcattatat
     1621 gtaccaacat atagagaaga taaagcagat aatagggcta ttgataaagc ttattttgaa
     1681 aaatgtttac caggatatac actgattaat aaactacatc catcaattga acattcagac
     1741 attgatgatg tatcttcgat cgatacgtct atattaatgt taatgtcaga tataattatt
     1801 agcgactata gttcgctgcc aatagaagct agcttgttag atattccaac tatattttat
     1861 gtgtatgatg aaggaacata tgataaggtg agaggcctga atcaatttta caaagcaata
     1921 ccggatagct acaaagtgta tactgaagag gatttaataa tgacgataca agaaaaagaa
     1981 catctattaa gtccattatt taaagattgg cataagtata atactgataa aagtttacat
     2041 cagctcacag aatatataga taagatggtg acaaaatgag gcttacgata atcataccta
     2101 catgtaataa tgaggcaaca attcgacaat tgttaatctc tattgagagt aaagaacact
     2161 atagaatcct ttgtattgat ggtggttcta ctgatcaaac aattcctatg attgaacggt
     2221 tacaaagaga actcaagcat atttcattaa tacaattaca aaatgcttcg atagctacgt
     2281 gtattaataa aggtttgatg gagatcaaaa tgacagatcc acatgatagt gacgcattta
     2341 tggtcataaa tccaacatca atcgtattgc cgggtaaatt agataggcta actgcagcat
     2401 tcaaaaataa tgataatatt gatatggtaa tagggcagcg agcttacaat taccatggtg
     2461 aatggaaatt gaaaagtgct gatgagttta ttaaagacaa tcgaatcgtt acattaacgg
     2521 aacaaccaga tttgttatca atgatgtctt ttgacggaaa gttattcagt gctaaatttg
     2581 ctgaattaca gtgtgacgta actttagcta acacttacaa tcacgaaata cttgtcaaag
     2641 cgatgcaaaa agctacagat atacatttag tttcacagat gattgtcgga gataacgata
     2701 tagatacaca tgctacaagt aacaatgaag attttaaaag atatatcaca gaaattatga
     2761 aaataagaca acgagtcatg gaaatgttac tattacctga acaaaggcta ttatatagtg
     2821 atatggttga tcgtatttta ttcaataatt cattaaaata ttatatgaat gaacacccag
     2881 cagtaacgca cacgacaatt caacttgtaa aagactatat tatgtctatg cagcattctg
     2941 attatgtatc acaaaatatg tttgacatta taaatacagt tgaatttaat ggtgagaatt
     3001 gggatagaga aatatacgaa ttgtggcgac aaacattaat tcaagtgggc attaataggc
     3061 cgacatataa aagattcttg attcaactta aagggagaaa gtttgcacat cgaacaaaat
     3121 caatgttaaa acgataatgt gtacattgat gaccataaac tgcaatccta tgatgtgaca
     3181 atatgaggag gataacttaa tgaaacgtgt aataacatat ggcacatatg acttacttca
     3241 ctatggtcat atcgaattgc ttcgtcgtgc aagagagatg ggcgattatt taatagtagc
     3301 attatcaact gatgaattta atcaaattaa acataaaaaa tcttattatg attatgaaca
     3361 acgaaaaatg atgcttgaat caatacgcta tgtcgattta gtcattccag aaaagggctg
     3421 gggacaaaaa gaagacgatg tcgaaaaatt tgatgtagat gtttttgtta tgggacatga
     3481 ctgggaaggt gaattcgact tcttaaagga taaatgtgaa gtcatttatt taaaacgtac
     3541 agaaggcatt tcgacgacta aaatcaaaca agaattatat ggtaaagatg ctaaataaat
     3601 tatatagaac tatcgatact aaacgataaa ttaacgtagg ttattataaa ataaatataa
     3661 aacggacaag tttcgcagct ttataatgtg caacttgtcc gtttttagta tgttttattt
     3721 tctttttcta aataaacgat tgattatcat atgaacaatt agtgctaatc cagcgactag
     3781 gcatgcacca ccaatgatag tgaataatgg atgttcttcc cacatacttt tagcaacagt
     3841 atttgccttt tggataattg gctgatgaac ttctacagtt ggaggtccat aatctttatt
     3901 aataaattct cttggatagt ccgcgtgtac tttaccatct tcgactacaa gtttataatc
     3961 ttttttacta aaatcacttg gtaaaacatc gtaaagatca ttttcaacat aatatttctt
     4021 accatttatc ctttgctcac ctttagacaa tatttttaca tatttatact gatcaaatga
     4081 acgttccatt aatgcattcc ccatcatatt acgttgcttc tcgccaccaa ggtttttata
     4141 gtctcctgca cccatgataa cttgattaat tctaaattta cctcgtttag tagtaatcgt
     4201 atggttgtaa tttgctgtat cacttgatcc agtttttaaa ccatctgtac caggcaaact
     4261 catttttgca ccttccaatg aaaagttgaa tgtgtaatac gtaactgcat gcgttgttgg
     4321 tgctaactgc tttgtaaagt ctaatatttt aggtgtctct ttaatcacgt gtaaatctaa
     4381 aatggcgtag tctctagcag tcgttacagt acgttcttgg tctttatatt ttgttggtgc
     4441 aaatgtacgt aatcttgaat tttcagcacc cgttggattg acgaaatgtg tatttttcat
     4501 tccgatagct ttagctttgt tattcattaa atcaacgaaa tcgctggtgt tttttgatac
     4561 cttcttagct aaaattaatg ccgcggcatt actagaatta gatactgtaa tttgtaatag
     4621 gtctgcgatt gtccatactt gtccaggata tagtttcgta ttactcaact caggtagtgt
     4681 agacataata tattctttgt tcgtcattgt gactgtgtca tcaagtgaaa gctgcccctt
     4741 atttacagct tccaatgtta agtacattgt cattaattta gtcatagacg ctggattcca
     4801 cttagtatcg atattgtatt gatacagtaa ttgtccagtt tgactaacat taacagcact
     4861 cgtcggttcg tatgcagccg acaaacctgc ataaccatat tgatttgctg cttgtacagg
     4921 ggttacgtca ctgttagcag cttgtgcata tggtgtcata atacttaatg ttaaacataa
     4981 aatgatgata atagatatta aatttttcat aaagcgttaa tcttcccttt tccaattctt
     5041 aaatattccc taaaagcaat ggtcattcct acttacggaa atcattgcta attcacttca
     5101 ccttaattaa attgttgaaa attaagtttc ttgcggttta tttgaaaaat aatgcaaata
     5161 tattacgtac gtagctaaag atgttataat gtttgtacga agagcaaact tactcaaaag
     5221 tgattaatta tcatgtttta atataaaaac tttgagaagt tattacaaaa aatgcaatag
     5281 aaatattcta tcatataaat gttatgagcg gtattttggg gcaatacttt atttgatttt
     5341 taaagttttg ttgggagaaa atatatgata gaaatgcatg tatctatcta aatgaattaa
     5401 ctataaattt caaacagaag aggtaaaact atgaaacgag aaaatccatt gtttttctta
     5461 tttaaaaaac tatcatggcc agtgggtctt atcgttgcag ctatcactat ttcatcacta
     5521 gggagcttaa gtggactatt agtgccactg tttactggac gaattgtaga taaattttcc
     5581 gtgagtcata tcaattggaa tctaatcgca ttatttggtg gtatctttgt catcaatgct
     5641 ttattaagcg gattaggttt atatttatta agtaaaattg gtgaaaaaat tatttatgcg
     5701 atacgctcag ttttatggga gcatatcata caattaaaaa tgccattctt tgacaaaaat
     5761 gaaagtggtc aattaatgag tcgattaact gacgatacga aagtgataaa tgaatttatt
     5821 tcacaaaagc tacctaactt attaccatca atcgttacat tagttgggtc actaatcatg
     5881 ttatttattt tagattggaa aatgacatta ttaacattta taacgatacc gatatttgtt
     5941 ttaattatga ttcctctagg acgtattatg caaaagatat cgacaagtac acaatctgaa
     6001 attgcaaact ttagtggttt gttagggcgt gtcctaactg aaatgcgtct tgttaaaata
     6061 tcaaatacag agcgacttga attagataat gcacataaaa atttgaatga aatatataaa
     6121 ttaggtttaa aacaggctaa aattgcggca gttgtacaac caatttcagg tattgttatg
     6181 ttgctaacaa ttgcaattat tttaggtttt ggtgcattag aaattgcgac tggtgcaatc
     6241 actgcaggta cattaattgc aatgatattt tatgttattc aattatcgat gcctttaatc
     6301 aatctttcaa cgttagttac agattataaa aaggcagtcg gtgcaagtag tagaatatac
     6361 gaaatcatgc aagaacctat tgaaccgaca gaagctcttg aagattctga aaatgtatta
     6421 attgatgacg gtgtgttgtc atttgaacat gtagacttta aatatgatgt gaagaaaata
     6481 ttagatgatg tgtcgttcca aatcccacaa gggcaagtga gtgcttttgt aggtccttct
     6541 ggatctggta aaagtacgat atttaatctg atagaacgta tgtatgaaat tgagtcaggt
     6601 gatattaaat atggccttga aagtgtctat gatatcccgt tatctaagtg gcgacgcaaa
     6661 attggatatg ttatgcaatc aaattcgatg atgagtggta caattagaga caatatttta
     6721 tacggaatta atcgccatgt ttcagatgaa gaacttatta attatgctaa attagcgaac
     6781 tgtcatgatt ttatcatgca atttgatgaa ggatatgaca cgcttgtagg tgaacgagga
     6841 ttgaaactgt ctggcggaca acgtcaacgt attgatattg ctagaagttt tgttaaaaat
     6901 cctgatattt tgttacttga tgaagcaaca gctaatctcg atagtgaaag tgaattgaaa
     6961 attcaagaag ctttagaaac attgatggaa ggtagaacaa cgattgtcat tgcgcatcgt
     7021 ttgtctacaa ttaaaaaagc cggtcaaatt atattcttag acaaaggaca ggtaacaggt
     7081 aaaggtacgc attcagaact gatggcatca catgcgaagt ataaaaactt tgtagtgtct
     7141 caaaaattaa cagattaaat ttatatatat aagtaagctt ggagcaagta cagatatacc
     7201 atcgagaaaa ttaaaatgtg gcacattgat ggatatagat gttaataaat tgcttcaagc
     7261 ttttgtctat tttaaatcat gtgagaagtt acgacataat aattcttaaa ttaatgaaat
     7321 cgatatttta agaaaaaaat gctcatggta taatacaagt tataagcaaa catacatata
     7381 ttaaatactg tagccacgag tcataattct tcatatttta catagcaatt taactgattt
     7441 tagagtccac ggtacagaag tttgatattt caatgtttct aaatttttaa aaaattaaat
     7501 cataggtggg tgccaaatgt ttttattaat caacattatt ggtctaattg tatttcttgg
     7561 tattgcggta ttattttcaa gagatcgcaa aaatatccaa tggcaatcaa ttgggatctt
     7621 agttgtttta aacctgtttt tagcatggtt ctttatttat tttgattggg ggcaaaaagc
     7681 agtaagagga gcagccaatg gtatcgcttg ggtagttcag tcagcgcatg ctggtacagg
     7741 ttttgcattt gcaagtttga caaatgttaa aatgatggat atggctgttg cagccttatt
     7801 cccaatttta ttaatagtgc cattatttga tatcttaatg tactttaata ttttaccgaa
     7861 aattattgga ggtattggtt ggttactagc taaagtaaca agacaaccta aattcgagtc
     7921 attctttggg attgaaatga tgtttttagg aaatactgaa gcattagccg tatcaagtga
     7981 gcaactaaaa cgtatgaatg aaatgcgtgt attaacaatc gcaatgatgt caatgagctc
     8041 tgtatcggga gctattgtag gtgcgtatgt acaaatggta ccaggagaac tggtactaac
     8101 ggcaattcca ctaaatatcg ttaacgcgat tattgtgtca tgcttgttga atccagtaag
     8161 tgttgaagag aaagaagata ttatttacag tcttaaaaac aatgaggttg aacgtcaacc
     8221 attcttctca ttccttggag attctgtatt agcagcaggt aaattagtat taatcatcat
     8281 cgcatttgtt attagttttg tagcattagc tgatctattt gatcgtttta tcaatttgat
     8341 tacaggattg ataggaggat ggataggcat aaaaggtagt ttcggtttaa accaaatttt
     8401 aggtgtgttt atgtatccat ttgcgctatt actcggttta ccttatgatg aagcgtggtt
     8461 ggtagcacaa caaatggcta agaaaattgt tacaaatgaa tttgttgtta tgggtgaaat
     8521 ttctaaagat attgcatctt atacaccaca ccatcgtgcg gttattacaa cattcttaat
     8581 ttcatttgca aacttctcaa cgattggtat gattatcggt acattgaaag gaattgtaga
     8641 taaaaagaca tcagactttg tatctaaata tgtgcctatg atgctattat caggtatcct
     8701 agtctcatta ttaacagcag ctttcgttgg tttatttgca tggtaatatg tcgaagagtg
     8761 actacgataa tacattttaa ctaaaaaaga tgtccaggca tgtcgtctat tgatataggt
     8821 gagatgcttg gactttttta ttattgatat aaagatattt aaatattttt aaagttatcg
     8881 aaattgatgc aatataaaaa ccaagtgcac atggtaatac acttggcttt tatgggaaat
     8941 gaatattatt gtacatatga cagtaaggac taggtacagt catagtactt cgagcaaaat
     9001 ttgttttgtt attataaaca acacaaagga gataacttct ctattgaaga agttaaaaac
     9061 attatagcag acaatgaaat gaaagtaaat taaaaattca gaatattttt aattattata
     9121 ttgtgagtga tatttattag ggaaagctat tcttcatata aattagttaa atagtaaatc
     9181 tttgttagaa ctctttccat agagttgtga cagtattttt gttgtgctac tatttttata
     9241 gtctaataaa tatataaagg ggatggtttc gtgaataaaa cggttaaaga tttaatacta
     9301 gttgtcttag gttcatttat ctttgctgca ggtgtaaatg catttattat ttctggtaac
     9361 ttaggtgaag gtggggttac aggtttagca attattttat attatgcgtt tcatatttca
     9421 ccagccatca ctaacttctt ggtcaacgca gcattgattg ccataggtta taaatttttg
     9481 agtaagagaa gtatgtactt aactattctt gtaacaattc ttatttcaat atttctgagt
     9541 ttaacagaat catggcaagt agaaactgga aacagcattg tgaatgccat ttttggtggt
     9601 gtaagcgttg gactaggaat cggagtaatt atccttgcag gcggtacaac agcaggtaca
     9661 acaattttgg cgagaattgc aacgaaatac ctcgatgtaa gcacgccata tgctttgctt
     9721 ttctttgata tgatcgttgt tgcaatttca cttacagtta ttccacttga taaagtatta
     9781 gtaacagtaa tatcacttta tataggaaca aaagtgatgg aatatgtcat agaaggttta
     9841 aacactaaaa aagctatgac gattatttca actaatcccg acaaacttgc caaagcaata
     9901 gacgagcaaa ttggaagagg tttaaccatt ttaaacggac atggctatta tacacgtgaa
     9961 gaaaaagatg tcttatacgt tgttatttct aaaacacaag tatcaaaagc aaagcgatta
    10021 attaaacaaa tcgataaaga tgcattcctc gtaattcatg atgtaagaga tgtctatggt
    10081 aatggttttc ttgcagatga ataaataaat ggtaagagca cacatactta aatagaagtc
    10141 cgcggacaag tttttgaatt atgaagactt atctgtggac gttttttatt ttataaaagt
    10201 aatatacaag acatgacaaa tcgagctatc caatttaaaa agtaatgtta gtcaataaga
    10261 ttgaaaaatg ttataatgat tttcatgata atcattatca attgggatgc ctttgaaaat
    10321 tgataattta aaaatagaaa ttatttttta taaacagaaa gaattttatt gaaagtaggg
    10381 aaattatgaa tcgtttgcat ggacaacaag ttaaaattgg ttacggggat aacacgatta
    10441 taaataaatt agatgttgaa ataccagatg gcaaagtgac gtcaatcatt ggtcctaacg
    10501 gctgcgggaa atctactttg ctaaaggcat tgtcacgttt attggcagtt aaagaaggcg
    10561 aagtattttt agatggtgaa aatattcata cacaatctac gaaagagatt gcaaaaaaaa
    10621 tagctatttt acctcaatca cctgaagtag cagatggctt aactgttgga gaattagttt
    10681 catatggtcg ttttccacat caaaaaggat ttggtagatt aactgctgag gataagaaag
    10741 aaattgattg ggcaatggaa gttacaggaa ctgatacatt ccgacaccgc tcaatcaatg
    10801 gtttaagtgg tggtcaaaga caacgtgttt ggattgcaat ggcattagca caaagaactg
    10861 atattatctt tttagacgaa ccaaccacat atttagatat ctgtcatcaa ttagaaatac
    10921 tagaattagt tcagaagcta aatcaggaac aaggttgtac aattgtcatg gttcttcatg
    10981 atatcaacca agcgattcgt ttctcagatc atcttattgc gatgaaagaa ggcgatatca
    11041 tcgctacagg ttcaacagaa gacgtattaa cacaggaaat tttagaaaaa gttttcaata
    11101 ttgatgttgt tttaagtaaa gatcctaaaa ctggaaaacc tttactggta acttatgact
    11161 tatgtcgcag agcttattct taattaagta agttaatatg ataaaaagga caattaacat
    11221 gacaaataga gagaatccaa cgccattgaa gtttttatcc tatattatag gtttaagtat
    11281 gatactacta atcacacttt ttatttctac attaataggt gacgccaaaa ttcaagcctc
    11341 tacaattata gaggctattt ttaattataa tcctagcaat caacagcaaa acatcatcaa
    11401 tgagattagg attccgagaa atatagcagc agtaattgtg ggtatagcgc ttgcagtttc
    11461 tggtgcgatt atacaaggtg ttactcgtaa tggtcttgct gatccggcgc tcataggttt
    11521 aaattcaggt gcttcatttg ctttagcatt aacatatgca gttttaccaa acacttcatt
    11581 tttaatattg atgtttgctg gatttttagg tgctattcta ggaggtgcta ttgtattaat
    11641 gataggccga tctagacgtg atggatttaa tccgatgcgt attattttag cgggtgcagc
    11701 agtaagtgct atgttaacag cgctaagtca aggtattgca ttagctttta ggttaaatca
    11761 aacagtaaca ttttggactg ctggaggcgt ttcaggcaca acatggtcac accttaagtg
    11821 ggcaattcca ttaattagta ttgcgttaat cattatatta acaattagta aacaacttac
    11881 cattttaaat cttggtgaat cattagctaa aggtttaggt caaaatgtaa caatgatcag
    11941 aggcatatgt ttaattattg ctatgattct agcaggtata gcagttgcta tcgctggaca
    12001 agttgcattt gtaggattga tggtgcctca tatagcaagg tttttaattg gaactgatta
    12061 tgctaaaatt ctaccattaa cagccttgtt aggtgggata ctcgtgcttg ttgctgatat
    12121 gatagcacga tatttaggag aagcgcctgt tggtgcaatc atttcattta tcggtgttcc
    12181 ttacttttta tatttagtta aaaaaggagg acgctcaata tgattagttc aaataataaa
    12241 cgcagacaat ggatagcact ggctgttttt agcattctac tatttctagg ttgtacttgg
    12301 agtattacct caggtgaata caacatacct gtagaaagat ttttcaaaac tttaattgga
    12361 caaggtgatg ccattgatga attaatctta ttagatttca gattacctcg gatgatgatt
    12421 actattttgg caggtgcagc gcttagtatt agtggtgcaa tagtgcaaag tgtcacgaaa
    12481 aatccaatag ctgaaccagg tatattaggt attaacgcag gtggcggatt tgcaatcgca
    12541 ttatttattg caattggtaa aattaatgct gacaactttg tttatgtact gccgttaata
    12601 agtatactag gtggtatcac cgctgcattg attattttta ttttcagttt taataaaaat
    12661 gaaggtgtta cacctgcgag tatggtatta ataggtgtag gtttacaaac agcattatat
    12721 ggtggctcaa ttacaattat gtcaaaattt gatgataagc aatctgattt catcgctgct
    12781 tggtttgcag gtaatatttg gggtgacgaa tggccatttg tcattgcatt tttaccgtgg
    12841 gtgttgatta ttattcctta cttactattt aaatcgaata cactaaatat tattcatacg
    12901 ggtgataata ttgcacgagg tctaggtgta aggttaagca gagaacgttt aatattattc
    12961 tttatcgcag tgatgttatc atctgctgct gtagcagtag caggttcaat ttcgtttatc
    13021 ggattaatgg gcccgcatat tgccaaacgt atcgttggac cacgtcacca gttgttttta
    13081 ccaattgcca ttttagtagg ggcatgttta cttgttatag ctgatacaat tggcaaaatt
    13141 atattacaac caggtggggt tccagcaggt atagtcgtag caattattgg cgcaccgtat
    13201 ttcttatatt taatgtacaa aacgaaaaat gtatagtgtc aaaggacaca acttattgct
    13261 atgaaaggca ctttattata tggcttttca tagcattttt tatttaatga gtcactcaag
    13321 actatttaca ttttcaataa tgaaccatta agttatcaag actgtcttat cataaatata
    13381 ttttataacg gtatcaggtt aattctttat gttagcgcat tcatttattc tgttttatac
    13441 tatgactgac aataccaagg aggtacaaca tgatgaaaaa gttaattaat aagaaagaaa
    13501 catttttaac tgatatgata gagggcatgt taattgcgca tccagagtta gatctgattg
    13561 ctaatacagt tatagtaaaa aaagctaaaa aagaacaagg tgtagcaata gtctctggag
    13621 gtggaagcgg acatgaacct gcgcatgctg gatttgtagc tgaaggtatg ctagatgcag
    13681 cggtttgtgg cgaagtattt acatcaccta taccagataa aatattagaa gctattaaag
    13741 cagtagatac tggtgatggt gtattactag ttgtgaaaaa ctatgcaggt gacgtgatga
    13801 attttgaaat ggcacaagag cttgcagaaa tggaaggtat aaatgttgaa acagttattg
    13861 ttcgtgacga cattgctgtg acaaatgaag tacaacgtcg tggtgttgca ggaacagtgt
    13921 ttgttcataa gcttgccggt tatcttgctg aaaaaggtca ttcattaaca gagattaaat
    13981 cgcgtgtaga agcgttgtta cctgaaatta aaagtattgg tatggcaatt gagccaccgc
    14041 ttgttccaac tactggaaaa tatggctttg atattgaaga cgacaaaatg gaaatcggta
    14101 ttggtataca tggtgaaaaa ggtattcata gagaagaagt aaaggatatt gatcatattg
    14161 ttggaacatt attagacgaa ttgtataaag aagtaactgc caatgaagtc atagtaatgg
    14221 taaatggtat gggtggtacg ccgttatctg aattaaatat cgtaactaaa tatattcaac
    14281 aaaatttggc tgcaagaacg gttaatgttg ctaaatggtt tgttggtgat tatatgacat
    14341 ctttagacat gcaaggtttt tctataacta tcgtgcctaa taaaccagaa tatctggaag
    14401 catttttagc accaacaaca agtcaatact ttaaataagc agtgaatatg aataaatata
    14461 catttatgag gtggcacaaa tgaaagtgaa tgatatgaaa gcgcgtttat taaatttaga
    14521 agaaactttt aaaaaacatg aatctgaatt aactgaatta gatcgagcaa ttggtgatgg
    14581 tgaccatggg gttaacatgg ttcgtgggtt tagtagtctt aaagacaaac ttgatgatag
    14641 ctcaatgcag tcattgttca aatctactgg tatggcattg atgtcaaatg ttggcggtgc
    14701 atcaggacca ctgtatggct ttagctttgt taaaatgtct gcagtcgcca aagatgatat
    14761 ggataatcaa gattttatta aactaattca ggcatttgcc gaagcggttg aatcacgtgg
    14821 gaaagttact ttaaatgaaa agacaatgta tgatgtagta gcacgagcag cagagaagct
    14881 taaaaatggt gaaactttaa catttaatga tttacagcaa ttagcagata atacaaaaga
    14941 tatggtagca acgaaaggta gagctgcata ttttggagaa gaatcaaaag gttatataga
    15001 tccaggtgct caaagcatgg tttatatttt aaacgctttg attggagatg aagataatgc
    15061 ctaaaattat acttgttagc cacagtaaag aaattgcaaa tggtacaaaa tctttgttaa
    15121 agcaaatggc tggtgacgtt gatattatac caatcggggg attaccagat ggttcaattg
    15181 gaacttcatt tgatatcatc caagaagctt tgactaaatt agatgatgat gcattgtgtt
    15241 tttacgatat tggatcttca gaaatgaatt tagatatggc aattgaaatg tatgatggta
    15301 atcatcatgt gttaaaagtt gatgcgccaa ttgttgaagg tagttttatc gcagcagtaa
    15361 agctgtcaat tggcggttca attgatgatg cattagcaga aatcaaacaa tcattttagt
    15421 taaaatttac taataatgaa aaatgtaaac ctttttcaaa tgaaacttta taaaaaatat
    15481 gatagtatat atgtaaatgt ttaataaaat ctggagaaat aggaggacat tgccatgcaa
    15541 caccttataa aaaaacatgt attgaatggc gagtttgatt tagtacgaca attgatgtcc
    15601 gaaacagatt ttatggaatt tgaagaagca tatatttcaa gtgcgcatga agtagaaagt
    15661 atgatgtttt atacatgtat tttagatatg attaagtacg aagaatcatc tgaaatgcat
    15721 gacttagcat ttttattgct tgtgtatcca ctaagtgaat atgaaggtgc tttggattct
    15781 gcttattatc atgcagacgc ttccataaaa ctaactgacg gcaaagaagt taaaagtttg
    15841 ttacaaatgt tattattgca tgcgatacca acacctgtta tttcagataa gaaggctttt
    15901 gatatcgcca agcaaatttt aaaattagat cctaataata atgttgctcg taacgtctta
    15961 aaagacaccg ccaaacgtat ggacaacgtt gttgttgata taaatgaatt acaccaacgt
    16021 aatgcacgtt aattacattt caattatatt agcttaataa tagttttaac atttggttgg
    16081 gttgggacat atgttccagc cttttttaat acttaaaaac taacgaagta aacttgtgtg
    16141 cacaaatggt ttttatacaa cattttataa atttatacat tttaataaag aacatacgat
    16201 agatagttta aaccttttta actgagaaat tttgatatgt attttcgaaa cttaactaaa
    16261 aatacgtaag tcaagaagca caattattaa tcatttttcc tataaaaaag ttcgtatgac
    16321 tgcattataa aagcataaat ttataatttt tttaaatgtc attgaacgtg ataatgtgaa
    16381 tggattgagc aattttgaaa aagtgaaaaa taacctatgc gacttgcaat taattttcag
    16441 tacgttataa tgcacactgt gcaaaattaa ggaggtctat tattcacatg atgatgaata
    16501 aagaagcaac aaaaattgga tttgcctacg tcggcattgt agtgggcgca ggattttcaa
    16561 caggacaaga agttatgcaa tttttcacta aatatggctt gtgggcttat ttaggtgtca
    16621 ttatatctgg ttttatttta gcttttattg ggcgccaagt agcaaaaatt ggtactgcct
    16681 ttgaagcgac aaaccatgaa tcaacattac aatacgtatt cggtgaaaag tttagtaaag
    16741 tctttgatta tattttaatc ttcttcttat ttggtatagc tgtaaccatg atagcaggtg
    16801 caggcgcaac atttgaagaa agttataaca tacctacatg gttaggtgca ttaattatga
    16861 cattagcaat ttatgtaaca ttgctattag actttaataa aatagtacgt gcccttggtg
    16921 tcgttacacc atttttaatt gttttagttg tattaatcgc tggcgtgtat ttatttaaag
    16981 gtcatgtttc attagcagaa gttaaccaag tagtgcctga agcaagtatc tggaagggaa
    17041 tctggtttgg tacaatatat ggtggattag ctttttctgt aggttttagt accatcgtag
    17101 caatcggtgg ggatactgaa aagcgtacag tgtcaggtgc aggcgcgatg tacggtggta
    17161 ttatctatac tgtattacta gcattgatca actttgcatt gcaaagtgaa tatccaacta
    17221 ttaaaaatgc ctcaattcca acattaacat tagccaataa cattcatccg ttaatagcaa
    17281 cagtgttatc tgttattatg ctggcggtta tgtacaatac tattctagga ctaatgtatt
    17341 catttgcagc acgttttaca gaaccgtata gcaaaaaata ccatatcttt attgttataa
    17401 tgatgatagc aggttattta ttaagtttcg taggatttgc tgaattaatt aataagttat
    17461 atacaattat gggatatgta ggcctattta ttgtagtagc tgtaattatt aaatatttca
    17521 aacgtaaaaa tgcggataaa aaacatattg cttaatatca tatgatggat atccgaaact
    17581 ttacaattga atcactttgt tttaatctta aaagcaattc gtctctactc ttatcgggcg
    17641 aattgctttt tatatttatt cagtctatta atatgagcgt ctaacaaata gagaggtacg
    17701 atgtaatgaa taaagataat aaatggacga tgataactgc gctttttata gcagtaatca
    17761 gtgtattgtt agcatttcat ctgaaacaac attatgacca aactacaaat gagaaccatg
    17821 ctaataaaga caaaattaat attaaaaata aaaatgtgcg catttatcaa aaccttacat
    17881 acaatagagt ttatcctaac agtaaattag atattattac acctgttgat atgtcttcta
    17941 atgccaaact gccagttatt ttttggatgc acggtggtgg ttatattgcg ggtgataagc
    18001 agtataaaaa cccattatta gcgaaaattg ctgaacaagg gtacattgtt gtgaatgtaa
    18061 attatgcatt ggcgccacaa tataaatatc ccacaccatt aattcaaatg aatcaagcga
    18121 ctcaattcat taaagaaaat aaaatgaatt tacctattga ttttaatcaa gtagttattg
    18181 gcggtgattc tgctggcgct caattagcaa gtcaatttac tgcaatacag acaaataatc
    18241 aattaagaaa agccatgaaa tttgatcagt cattcaaacc atcgcaaatt aaaggtgcta
    18301 tactatttgg tggtttttat aatatgcaaa cagttagaga aactgagttt ccgcgaatac
    18361 aattatttat gaaaagttat acaggtgaag aagattggga aaagagtttt aaaaatattt
    18421 cacaaatgtc gacagtaaaa caatcgacaa aaaattatcc accaacattt ttatctgttg
    18481 gagatagtga tccattcgaa agtcaaaata tagaattcag taagaaatta caagaattga
    18541 aagtaccagt agatacattg ttttatgatg gtacgcatca tttacatcat cagtatcaat
    18601 ttcaccttaa taaacctgaa tcgatagata atatcaaaaa agtgttactt ttcttaagtc
    18661 gtaatacatc ttctagtggt attcaaactg aagagaaacc acaaatagaa aatccgagta
    18721 atgaattacc gttaaatcca ttaaactaat gataaacagt agtaatttat tacttaagca
    18781 acatttaaga tcttcaaatt gaaaacgaag aatttaaaac atgtggggct aatgtgtaag
    18841 tgtctgagat ataataaatt gttagcggtt ctttatcatt tctgtccccc cctattatga
    18901 tgtgacattt atttacaaac taatttgttt tgaacctgaa aataactttt tacaactaaa
    18961 tagtcgaaaa acagtgtgta catgatataa taaatttata aattgaaaag aattcaaaag
    19021 aaaaattaaa atatagattg agcacgaagt gatttgaaat aaggttgtga aagggaatga
    19081 caaggtcagc attaaaacca tttaaaaata aacgcgttat ggttactgga cgtatacatc
    19141 gtgtgttgtt taaaaattat ttagatagac atagcacatt taagccgaat gtaaggatat
    19201 tattaaaaga tgtaattgtt tcaggtgttt caatagatca tttatggcta tatgagacaa
    19261 ataaatacta tgcattagca atggaactta ttcatcaacg agtaaaattt agtgcgaatg
    19321 ttgtaccgta ttacaaaata aatagaaata ataatttatt cgtacaagat tatggaatta
    19381 agcgtaaagg taatttagtt actgaagaag cttacattca aaacaatcag tatcaggata
    19441 aaatatatga aaaattaccg gatat
//
LOCUS       DAKSQE010000046        19246 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.046, whole genome shotgun sequence.
ACCESSION   DAKSQE010000046 DAKSQE010000000
VERSION     DAKSQE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19246
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.046"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            1..593
                     /locus_tag="O0V33_001361"
                     /pseudo
     CDS             1..593
                     /locus_tag="O0V33_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373851.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(885..1082)
                     /locus_tag="O0V33_001362"
     CDS             complement(885..1082)
                     /locus_tag="O0V33_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976000.1"
                     /translation="MNCYDEIYNTIKKLIENKEITSYQINKDTGISYGNINAMRRGER
                     RIENLSLKNAKILYEYAKKVL"
     gene            complement(1576..2916)
                     /gene="glnA"
                     /locus_tag="O0V33_001363"
     CDS             complement(1576..2916)
                     /gene="glnA"
                     /locus_tag="O0V33_001363"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCX8976001.1"
                     /translation="MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILESGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            complement(2935..3303)
                     /locus_tag="O0V33_001364"
     CDS             complement(2935..3303)
                     /locus_tag="O0V33_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCX8976002.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
     gene            complement(3547..4785)
                     /locus_tag="O0V33_001365"
     CDS             complement(3547..4785)
                     /locus_tag="O0V33_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCX8976003.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGISERTKV
                     IAIQRSKGYDQRPSIPLNEIEQVITRLKEVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFKTKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVMQLKEQGLI"
     gene            complement(4804..6042)
                     /gene="hflX"
                     /locus_tag="O0V33_001366"
     CDS             complement(4804..6042)
                     /gene="hflX"
                     /locus_tag="O0V33_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCX8976004.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKLQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDEAIATNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            6154..6630
                     /locus_tag="O0V33_001367"
     CDS             6154..6630
                     /locus_tag="O0V33_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCX8976005.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRFAPQKKPVQIEREIEKLL"
     gene            complement(6852..7085)
                     /gene="hfq"
                     /locus_tag="O0V33_001368"
     CDS             complement(6852..7085)
                     /gene="hfq"
                     /locus_tag="O0V33_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCX8976006.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEAQESTESEE"
     gene            complement(7100..8035)
                     /gene="miaA"
                     /locus_tag="O0V33_001369"
     CDS             complement(7100..8035)
                     /gene="miaA"
                     /locus_tag="O0V33_001369"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCX8976007.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIGILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSVVKQKLSALEHLDNQQLHEYLAQFDEASAKNI
                     HPNNRQRVLRAIEYYFKTKKLLSNRKKVQQFTENYDTLLIGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            complement(8053..8967)
                     /locus_tag="O0V33_001370"
     CDS             complement(8053..8967)
                     /locus_tag="O0V33_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCX8976008.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYPLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPTEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNIKKLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            complement(9117..10790)
                     /locus_tag="O0V33_001371"
     CDS             complement(9117..10790)
                     /locus_tag="O0V33_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HCX8976009.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVNVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDSIIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            complement(10948..12444)
                     /gene="glpK"
                     /locus_tag="O0V33_001372"
     CDS             complement(10948..12444)
                     /gene="glpK"
                     /locus_tag="O0V33_001372"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCX8976010.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKP
                     SSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAYLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
     gene            complement(12574..13392)
                     /locus_tag="O0V33_001373"
     CDS             complement(12574..13392)
                     /locus_tag="O0V33_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCX8976011.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVYAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATVYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
     gene            complement(13867..14409)
                     /locus_tag="O0V33_001374"
     CDS             complement(13867..14409)
                     /locus_tag="O0V33_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCX8976012.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
     gene            complement(14415..16424)
                     /gene="mutL"
                     /locus_tag="O0V33_001375"
     CDS             complement(14415..16424)
                     /gene="mutL"
                     /locus_tag="O0V33_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCX8976013.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLHSFEQQKIEFEQRQNTENKQEKTFSSEESNSKPFMAENQNDEIVIK
                     EDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDDTSVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEIQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            complement(16437..19055)
                     /gene="mutS"
                     /locus_tag="O0V33_001376"
     CDS             complement(16437..19055)
                     /gene="mutS"
                     /locus_tag="O0V33_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCX8976014.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDHLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDAVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQDTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNMQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQHQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENSNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
BASE COUNT         5901 a         3608 c         2631 g         7106 t
ORIGIN      
        1 taataagtct tctgaaaagc gtggcaaatt agacgtaggg tctcgtgtta taaagtatcg
       61 gctcaaaaag tattaataat cgtgaagaat ttggacatta tgaaattgat actgtttgga
      121 gtgttagacc atcaacatat tgcttgttaa ctattattga aagaaaaaca agatatttat
      181 atgctagtag attaaataca cgtaaatcca atgtagtttg taatgagatt ataaatatta
      241 tgaaaccatt attacccaaa agtataacta tggatagagg taaggagttt gctctattta
      301 aacaaataga agatgaattg aattgtagta tttattttag tgatttgggt tgtccatatc
      361 aacgtggttc cattgaaaat gcaaatggac ttttaagaca gtattatcct aaaggtacgg
      421 attttgcaaa tataacacaa aagtcattag atgaagctgt taaacaaatt aatgcacgac
      481 ctcgaatgat atttgactat aaatcgagtg aggaaatgct aaagtaccat gtaagcacac
      541 aaaattgtga acctatatta aatgactgtg tgaggcacga acccgtcaat taatataggt
      601 tcatctaagc gaatggttta caatgagcgc ggaaggacag gacgaataaa ttttgtatat
      661 aaaatgcata atctaaaaag aagcatacta tttaaatctg tggtttttga cagatattac
      721 tagtaaaatg cttctttttc ttcttctgta ctcttttagt aatatttatt aaaaaaattt
      781 aagaaattct catttttcta ttgacacact gccatactat aaaaacaccc actcagtcac
      841 tagtttgggc agttattgta tgcctattga actcaatgct tatattataa tacctttttc
      901 gcatattcat ataagatctt tgcattcttt aagcttaaat tttctattct tctttctcca
      961 cggcgcatag cattaatatt accgtaactt atcccagtat ctttattaat ttgataactc
     1021 gttatctctt tgttttctat caattttttg attgtattgt atatttcatc atagcaattc
     1081 ataaattaga tgaggcaaaa atttttaatt ttttagaata tcaatagtag tataactaaa
     1141 atgaaaatac cgatcgataa acaaaaagat attttttgtt ttgtttctct tttcatatag
     1201 tattaccccc ttaataatga gtagtaaggc ccctattttc ggggtcttac cttagaacgt
     1261 tctgcgaatg aattcgatga gaagtaatat gaatgtggct attttcaagt aatactcaac
     1321 gttttcgcga cgttctttta tcacctcatc tcatcacctc caaatatatt aaaattcatg
     1381 tgaactaaaa tataaaatgg tcttccccag ctttaaaaaa ataaatacat aaaacattta
     1441 cttgaccaaa acttgaccaa atccattttt ttacaaaagt ggtcaagtga aattccacct
     1501 ttccaaatca aaaaagagct aaagcaaaac gctctagccc ttgatattac tgatttccca
     1561 tcattgttta gcgtattaat attgcttcat gtactgatct ctttcccatt cagatacttg
     1621 agttctatag taatcccatt caattgattt tgaattaata aattgattat agatatgatt
     1681 tcctaaagct tttttaataa cttcattttc acgcattgct tttaatgcag tataaagtgt
     1741 tgaaggtaag tcttgaatgc ctactgcttc acgttcttca cggttcattt cgtaaatatt
     1801 ttggttaact ggttctggaa cttttaattt atttttaatg ccgtctaaac cagattctaa
     1861 aattgcagct aacgccatat atgggttcgc agctgggtca actgaacgta cttcaatacg
     1921 agttgataat cctcttgaag atggtacacg gattaatggt gaacggtttt taccactcca
     1981 tgcaatataa catggcgctt cataaccagg tactaaacgt ttatatgagt ttactaacgg
     2041 gttacataca gcagtaaatc ctcgtgcatt tttaagtaca cctgctgtaa attgatatgc
     2101 tgtttccgtt aagcccattt cagtatttgg atcgaagaat gcattttctt tacctttgaa
     2161 taatgaaacg ttaaagtgca taccgctacc attcacacca aataatggtt taggcataaa
     2221 tgttgcgtgt aaattatgtt tacgtgcgat tgttttaaca accaatttaa atgtttggat
     2281 attatcacat gctgtaacag catctgcata tttaaagtca atttcatgtt gaccaggggc
     2341 aacttcatgg tgactagctt caatatcgaa gcccatatcc tctaattcta aaacaatatc
     2401 acgacgacag ttttcaccta aatctgtagg tgctaaatcg aaatatccac catcatcatt
     2461 aagttctaaa gttggttccc ctttttcatc caacttaaac aagaagaatt ctggttcagg
     2521 ccctaggtta aagtctgtga agcctaaatc ttccatttct tttaatacac gttttaagtt
     2581 tgcacgagga tccccttcaa atggtgttcc atctgtttta tatacatcac aaattaaacg
     2641 tgcaacttta ccttgtccag cagtccatgg gaagattacc caagtatcta aatctggatg
     2701 taagtacata tctgattctt cgatacgtac gaaaccttcg atagaagaac cgtcaaacat
     2761 catttcgtta tcaagtactt tttctaattg gcttacaggc acttcaacat ttttaattgt
     2821 tcctaaaata tcagtgaatt gtaatcttaa atatcttaca ttttcctctt ctgcaaattt
     2881 acgaatgtcg tctttagtga aagtacgttt tggcatttta aaatcctcca aattttattt
     2941 aataaatcgg gataaatcac cacgatttat tggcaaagtt tctccaatag gcttttgcgt
     3001 ggcatctaca atcatctttt ttcttatctc ttgttcatct gttgttaaat gctcttgtga
     3061 gtcataaatg atttgtttaa tccctttgat attaaatcct ttttctaata atgatttaat
     3121 ttctagtaat ctttccaaat cattgagtga gaacagacgt ttttgacctt ctgttctttc
     3181 aggtttgatg agttcatgtg tttcatagta acgtatttgc cttggcgtta aatccgttaa
     3241 cttacttact acactcatag agaagacagc catatttcgt ctgattgcat cattcgatat
     3301 cattgttcct ctcctctact tttgaacttg ttaataactt atcataattt ttttaggatt
     3361 tcaaaaatat gtcaggtttt ctaacataaa gctcaaaaca ttgatataac tgcgatttcg
     3421 acctctaaaa cgccataaaa cgacctacag ctaaaatatt ttttcaattt ttaaaaataa
     3481 tctgataaat attcgcaaaa aaatttcacc caaaacatat tatcatttag ggtgaaattt
     3541 aatctattat ataagtcctt gttctttcaa ctgcataaca gctcttgtca cagcaatttt
     3601 aacgtgttca tatgttaatc ctccttgaac atatgcttca taaggaggac gaataggtcc
     3661 atctgcagat aattcaatcg atgaaccttg aataaacgta ccagctgcca taataacatc
     3721 atcttcgtaa ccaggcatat aactaggttc tggactaaaa tgtgcattaa ttggggatgc
     3781 gtgttgaata ctttgacaaa atgaaatcat ttgttctttc gttttaaatt taactgtttg
     3841 aattaaatca gttcgttttt cgtagtactt cggcgttgtg ttcatattca ttttttctaa
     3901 aaataaacta gtaaacaatg cacctttaag actctgactg acaacgtgtg gtgctaaaaa
     3961 gaaaccttga tacatttcaa gcaatgcatt taatgatgca cccgcttctt taccaatacc
     4021 aggtgctgtc aatctataac cacatcgttc aattaaatct tttctaccag caatgtatcc
     4081 accaatctta gctaaaccac cgccagggtt tttaattaat gatcctgcta ttaaatcggc
     4141 accacattct ataggttcac gtctttcaac aaattcccca taacagttat ccacaaatat
     4201 taaaatatta ggatgcactt ctttcaacct agtaatcacc tgttcaattt catttagagg
     4261 aattgaaggt ctttgatcat agcctttcga acgttgaatc gctattactt tggtgcgctc
     4321 agaaatccca tctaacacac tttcaatatc gatcttacct tctttaagtg caatatcttt
     4381 atatgatacg ccgtgctcca ttaaactttc aataccattt ccgtttacac caatgacttc
     4441 aagtaaagtg tcatatggac ttcccgttat ataaattaat tcatcaccat gttttaataa
     4501 actttgtaat gcaatagtaa tcgcatgcgt acctgaaata atttgtgggc gaacaattgc
     4561 atcttctgct ttaaatgcct gcgcatatat ttcttctaaa tgatcacgtc caaaatcatc
     4621 atacccgtat cctgttgatc cttgtagatc actttcggtt gctttgacat gatgaaatgc
     4681 atttaatact ttttcttggt taatatatgc agtttcttca atttctttaa aatatggtgc
     4741 taacgttgat tcgacgtcag ctactatctt acttatatct ttcatttgaa tttcgtcctt
     4801 ttattatttt ttaaatcctt ttatacgata aacattttca atttcatcat atttaagttc
     4861 agtcactaat gtatgttgtt ttagaaaata taatcgatct gcattattcg tcgcaattgc
     4921 ctcatcataa taagttaatt gccttttgat ttcatcaata aataaagtct taacaagtaa
     4981 tttatcacca tcatttttag atgaaacaaa aacattaggc aaatcacttg ctggacgatt
     5041 tgatgcatga tcacataagt cctttttatt aaaaataact atttgagaaa tatgactcat
     5101 atctaattgt ttgattaaat cattaactgt gtcatactgc gtacggtatt caggatggct
     5161 actatctacg acatgtacta ataaatctgc acctttagcc tcttctaaag ttgatttaaa
     5221 agctgcaatt aacgtcgtag gtagtttctg tataaaacca acagtatctg aaataattaa
     5281 attaaatcca tcatttattt gaatttgtcg tgttttagga tctaacgttg caaataattg
     5341 atctttttca tacgtctctt catttgctaa aacattaaac catgatgatt taccagcatt
     5401 tgtataacca actaaagcta cttgaaacac ctgattttga tttcttttat ttcgatatct
     5461 ttcgcgatgt tcttctaccg ttcgcaattg aagttttatt tcattcatac gagttcgaat
     5521 atgtctacga tccatctcta acttcgtttc accagggcct ctagttccaa taccgccacc
     5581 taaacgagaa agacttttac catggccttg caatctaggt aataaataat caagttgtgc
     5641 tagctctact tgcaatttac cttctttact tcttgctctt aatgcaaata tttcaagaat
     5701 caactgagtt ctatcaataa tttttacacc taaagcttca tttagtgatt tggattgtgc
     5761 agtcgttaat tcatcatttg tgatgactac atcaatatct ttgaactcaa taaatgcttg
     5821 aatttcttca attttacctt taccaacata atatttgcga tctacacgat ctctgttttg
     5881 agtaatttga cccaacactt caagttggca agtctctgat aaagatgata attcctccat
     5941 tgtagactca aaattaaatt gcttatcatc ttgagcatgt acaccgacta atacagcttt
     6001 ttctagttta tttttagtat catgaatttg ttgctgagcc atgttataca tctccttaat
     6061 aaaatcctac tcaaagtatt ctatcataga gttttagtaa agactattac acgattttat
     6121 ggtacatttt aagtaaatcg aaaggatgat tcaatggaga caatttatga ttttgtagta
     6181 gaaacaaata aaggtgttac ttacaaatta gatgcatata aaggtgacgt tatgctaatt
     6241 gtcaatacag ctagcgaatg tggctttact tcacaattcg aaggtttgca atcattatac
     6301 gagaaataca aagatcaagg gtttgtaatt ctaggtttcc cttgtaatca atttggtggc
     6361 caagaacctg gttcaggcga ggaagcagct caaaactgta aattaaacta tggtgttacc
     6421 ttccctatgc atcaaaaaat agacgtaaaa ggggaacatc aattaccttt atttagatat
     6481 ttgactgcgg cacaacatgg attctttaat gagaaaatca aatggaattt cactaagttt
     6541 ttagtggatc gtgaaggtaa cgttgttaaa cgatttgcac ctcaaaaaaa acctgttcaa
     6601 atcgaaagag aaatagaaaa gttattataa aatatactag ctcatagcaa ataaaacgct
     6661 tcatataaag tcgtatcata tattgactcg acctttatat gaagcgtttt tattacaaac
     6721 attcctctat tgtatcttgt catttgagca tcatttaatt gatgtcacat atgactttat
     6781 ttctatatct atttatcata cttcaaacgt tatgtacacg agtcattaaa tcgtgcccat
     6841 atgattacaa cttattcttc actttcagta gattcttgag cttcagtttc tactgtataa
     6901 gtgctgatcg catgtttgta aatcaagtgt tgtttgcctt gagaatttaa gcttacgaca
     6961 tacttgtcgt attcttcaat aacacctttc atttggaaac cgtttagaaa gaatacagtt
     7021 acttcagttt ggtttgcttt aaaattctct agtgctttgt cttggatgtt ttcgtttgca
     7081 atcatctgtc ggactccttt tacttaatct gggttgtaat ctcatctaac atcatttgaa
     7141 gtgacatatt ttctttatct aaccaatgaa cactcatttt attcttgaac catgtcattt
     7201 gtcgttttgc atattggcgt gaatgttgct ttaaatcatt gacagcatct tcataaatca
     7261 tttgtccgtt aatcacaggt attaattctt tatatccaat agcttgcata ctttggcaag
     7321 attcatagcc ttgttcaaca agttgttgca cttctctaaa taatccgtga tccaacataa
     7381 tatcaacacg tttatttatt cttgaatata atgttttacg cgacatttca atccctatta
     7441 ataatgtatc ataattttca gtaaattgtt gcactttctt acgattactt aaaagttttt
     7501 ttgttttaaa atagtattcg atagcgcgca atactctctg tcgattgttt ggatgaatat
     7561 ttttggcaga agcctcgtca aattgagcta aatattcgtg tagttgctga ttatctaaat
     7621 gttctaatgc agataacttt tgtttaacta ccgataattg tgcaggtgta actgtttcat
     7681 cttctaattc ataattatat attaatgatt gaatatataa gcctgttcca cctgctatga
     7741 ttggaacttt acctctattc gttatatcag taattaaatc ttctgctaat cgcttgaatt
     7801 catatgctga aaatgtatca tcaggattca agataccaat taaatgatgt ggaataccat
     7861 ccatttcttc aggtgttact tttgcagttc caatattcat atgtttgtag acttgcatag
     7921 agtcaccgct tatgatttca ccattgatac gcttcgcgag ttcgatgcta agctctgttt
     7981 tacctgaagc agttggcccc acaattacta caataaaagg cttattttta ttcatattta
     8041 attccacttt ctttattttt tataaaattg attcaaccat ttaaacaagt cttcccaaat
     8101 tttgtcatga tctttttcaa ataacacttc atgacgttta tgatgataaa gattaacttt
     8161 aacattttta atgccggctt ttctatatat attcgccaat tttaaaatcc ctttaccata
     8221 atcacctaaa ggatcgtcat atcctgaaac taataatata ggcatggcat gatttaactt
     8281 ttttatattt tttaattgtg atgtatgtag catataatat gctgtttgat ataataattg
     8341 atttgacaca ttaaatccac tatatggatc tttaataaag ttgtctactt cagttggatt
     8401 actagaaatc caatcacttt gtgtacgtaa tggacgtatt tttttattaa aactatttga
     8461 tactaactgg ttaacccatt caacccgttt ctcggcacca taaatttttg taatcagttg
     8521 taacacctta acggttggta atcctttcca taatggatac ataccagtac cacttaaaat
     8581 tagaccatca acatattgcg gatatgtttc tacaaacaat ctagctataa cggatcccat
     8641 tgaatgtccg attataatgt atggtttatc aacattgcct ctcactgttt gcgctacttc
     8701 aaaggcatca ccgataactc gtttcatatc atcgtaatgc cctcttgttg attcatcaat
     8761 attaatacca tgtcctcgat gattatgacg tatcacatcg aagccatgct tagaaagtgc
     8821 atgtgctaat ttatcgtaac gttccatatg ttcagccata ccatgaaata tatgaataat
     8881 tccttttgaa tctacatctg taaaattcac tttaacttca atattcgtat tatcttgtac
     8941 agttaattta aattgttttt cagtcatcat ctcacacctt taataacaac gtttttatat
     9001 tcgcttttat tattatcata catttagtga tgcatagttt caattgacta atgtatctca
     9061 tataaaaaaa ctgatgccct taaaacagga caccagttta tgattgtaca aattaattat
     9121 tctttaactg ctggttgatt atttccatgt tgcgcttctt taattgcttg ctcaacttct
     9181 tcagtataag cctcaatttg agctggagaa tagtctaaca tatcagccat aatatcgatg
     9241 atactatctt tataatctaa tacatcttta atattgaagt acattttacc agaacgacga
     9301 actaagaaat cgttaggttt gtataccatt tcttgttgaa tactataaac aagttctaca
     9361 taaatttcta atggtaactt gctgtcatgg tattgagatg tttgcgcaat gttgaacaat
     9421 tcatcaacat ttgaaccata tttagatgct aaacgtctcg caacatcttc atcaatgccg
     9481 aatcctttag ctacatctac tttttgctct acaaacgcat caaagttctt gctaccacct
     9541 acatcgccac cagaaattgc cagacctttt gtattacatg gactaaatgt taaaccatag
     9601 tcttttttca agcgtttaga tactaaatca acaatgtctt gagccatgtg acgatagcct
     9661 gttaatttac cacctgcaat agttaataaa cctgatttac cttcccaaat ttcatcctta
     9721 cgagagattt cagaagggtc tttgccctct tcgtaaatta atggtctaat tcctgcccat
     9781 gttgattcaa tatcttcatc tgtaacatta acactaggga acatgtaatt aatcgcatcg
     9841 attaaataat ctctgtcttc ttgtgtagtt aatggtgaag atttgatatt gtcatagaat
     9901 gtatctgtag tacctacata cgcttttcct tcacgcggga ttgcaaaaat cattcttcca
     9961 tctttttcag tatcaaagta tactgcttga cctaatggga attttgattg atcaataaca
    10021 acatgtacac ctttagttaa acgtaatttt ttattattgc gtgcataatc accacttcta
    10081 acatcatcaa cccatggacc tgctgcatta accacttttt tagccttaat tgtataattt
    10141 tcattagtta atttatctat aacattaaca ccatttactt gttgattttt atcataagtg
    10201 aagtgttctg acttagtata gttgataatt tctgcgcctt tttcggcagc acgcttcata
    10261 acttcaatag ttaaacgcgc atcgtcagta cgatattcaa catagtaacc gccgcctttt
    10321 agaccttctt ttttaactaa tggttcttta gctaaagttt cttttttaga taacattttt
    10381 ttacgttcag acttcttaac acctgctaaa cgatcataca ttcctaaacc aattgatgtt
    10441 gagaatttac caaatgttcc acctttatgc attggtaaaa gcatccactc tggagtcgta
    10501 acatgaggcc cattttcata aacaatcgca cgttctttac cagtttcggc aactactcct
    10561 atttggaatt gttttaagta acgtaaacca ccatggacta attttgtaga tcttgagctt
    10621 gttccttgtg caaagtcttg catttcaact aatgcaactt tcattcctct ttcactcgcg
    10681 tctagtgcaa tacctgcacc tgtaataccg ccaccaataa ttactaaatc atattcatca
    10741 tttcttaaat tctttttaat atgttctctc ttaaaagtag acaatgccat tccaaaacgc
    10801 ctcctaaaaa atgtaaaaac gagagacttg tcccacataa acaatccttc aattgtacat
    10861 gttcgaacaa atctctcgtt tctcgattca aatattaact taaattgatt gtatcacaat
    10921 gtttatacaa aagtctaatc tacaagttta ttctgtttta aaaacttgtg ttgcttcaac
    10981 agcttttttc caacctctat ataatttttc tctttcgcct tcatccattt tcggatcgaa
    11041 tttttcttct aatttccagt ttttagcgat atcatcttta ctctcccaga atccaactgc
    11101 taaacctgct aaatatgcag cacctaaagc tgtagtttct tgaatttcag gtctttcaac
    11161 agaagtatta acaatgtctg cttggaactg cataataaag ttattcttaa ctgcaccacc
    11221 atcgacacgt aaactttgga catcgatacc agagtctttt gacattgctt ccataacgtc
    11281 acgagtttgg taacataatg attctaatgt tgcacggata aagtgctctt tttcagttcc
    11341 acgtgttaaa ccgaaaatcg caccacgtgc ttcagaatcc caatatggtg ttcctaaacc
    11401 tacaaaagct ggaacaacat aaacaccctc agtagagtca actcgtgtcg cataactttc
    11461 tgattgtggt gctgaattaa tcattcttaa tccatcacgt aaccattgga ttgctgaacc
    11521 cgaaacaaag atggaacctt caagcgcata atttactttt ccatcaatac cataagcaat
    11581 tgttgttaat aaaccacttt cagatttaac cgctttgtca cctgtattca ttaacatgaa
    11641 gccaccagtt ccatatgtgt ttttcacgtc accacgttcg aagcaagctt gtccaaataa
    11701 tgctgcttgt tgatcaccag ctacaccagc aattggtact tcttgaccat agaagtggta
    11761 atcaattgtc ttaccatata cttcacttga aggtttaact tctggcaaca tatttttagg
    11821 tactgtaagt agttctaata actcatcgtc ccattctaaa tcatggatat taaacattaa
    11881 tgtacgactc gcatttgaat aatcagtaat atgcgcggct tttcctgata atttccatac
    11941 taaccaagta tcaatcgttc caaataatag atcgccattt tctgcttttt ctcgtgcacc
    12001 ttcaacattg tctagaatcc atttaacttt tgtacctgca aaatacggat ctaaaagtaa
    12061 tcctgtctta tctctaaatg tttgttcata tccttgttgt tttaattctg aacaaattga
    12121 ttgtgtttga cgtgattgcc aaacaattgc gtgataaatt gggcggccag tatgtttgtc
    12181 ccaaacaacc gttgtttcac gttggtttgt aataccgata cctgcaattt gatcagctct
    12241 aacatcattt tcattaatta cttccgtcat tacagctaac acagatgtcc aaatttcatt
    12301 tgcatcatgt tcaacccaac ctgattgtgg aaaatattgc ttaaactcac gttgtgctac
    12361 ccctgcaatt tccccttttt gattgaataa aatcgctctt gagcttgttg ttccttggtc
    12421 tatagataaa atatattttt ccattagtag tatctccttt attaaaaaaa taatcgctaa
    12481 cccattgtac aaaatccgtt aaattttggc gaaaaaatag cataatccat gaatcaaaca
    12541 tgttaaatta tgctatttac atttcttttt attttagtaa attgattcga tatcaccttt
    12601 ttttgatgat ttatttaaaa tgtaacctaa aatcaaagta ataataacta caacaattgc
    12661 aattgcacaa ccaatattaa atgtatgttt ataaaataca gcgtatacta ccgcacctaa
    12721 taaaccaccg gcaattggtc ctaagatagg aacgattgca tatgaccaat ttgaaccacc
    12781 tttgccagct attggtaaaa tcgcatgtgc aattctcgga cctaaatcac gtgctgggtt
    12841 gattgcataa acagtagcac cgcctaaact taatccgatt gcaacaatta atgctccgac
    12901 aattaaagga tttaaaccat cagcaatttt gtttacaccg ataaataaaa tacctaaagt
    12961 taatgccatt gttccgataa tttcacttaa aaagttggca aagtaattct taatagccgg
    13021 tgctgtagag aaaacaccta atttcgcgcc agcttcttct gtcgctttcc aatgtggcaa
    13081 gtacattaac catacaattg ttgctccgac aattgcacct aacatttgag caacaatata
    13141 accaggaact aatgaccaat caaaacttcc gtctaatgca agagctaaag acaccgctgg
    13201 gtttaaatgt gcacctgaga attgaccaac agcatacaca cccattgtaa ccgctaatcc
    13261 ccatccagct gtgatgacaa tccaatcagc accattcgca gcacttctct ttaaattgac
    13321 attggcacaa acgccaccac caaaaaggat taagattgca gttcctagga attctgctaa
    13381 atatacattc atattaaatg tcctcccaaa aagacaaaga gattcctaca ctagcagttt
    13441 ggctcgtaga aatctcccgt ctctattcta tgtattaact tttattatat ttgttataat
    13501 actattgaga tgaaagcgtt gtcaatggtt tttgtaaaaa ttttgtcaaa tttaattttt
    13561 caagtcttta taaaagtgat ttaatttgaa aactagaaat atcaatccta actaaattat
    13621 ttatgacgcc attttgttta aatgacattc ttcagccata ttttttatga ctagttgcat
    13681 tttttacaaa taaacacctc tataacttta atgattttaa tcgttttaga ggtgctaatt
    13741 attttattta attattcttt gtatgatacc ttaaattcct tattacatat cccatatgaa
    13801 aacggctttt ttaatatgtt atatgactaa atctagtgaa aaatctaatt tttgcagact
    13861 tatgattcac cagagtttat cataactagt tgttacatat ttcgctccat ttttaacagc
    13921 ttcattaact tcatctattg tattaattag gccacctgca atgacttgtg tgtttgtttc
    13981 tttctgaata tgatgaatcg ctttactcgc aacacccgga agtacttcaa caaaatcagg
    14041 ttcaactttt tttatcaaat ctatactgcg tttcaatgct tgactatcaa taataaacac
    14101 tctaaaaatc gttaaagtat ttaatgattt cgcttttttt attactttag atttagtcga
    14161 tacaatacct tttggcttgt attgctgaat aataaattca cttgcaaatt catcgtgact
    14221 taaacctttt atcaaatcta tatgaataaa acactctata tgattttgct tcagcaattc
    14281 cataatactt tttatatgtc ctatatgcat atctagaagc acacacattt tataatctgt
    14341 tttaatcagt ttctctaaat ctttaatgtt tcttatggca ggcaatatgt tgttattcac
    14401 ttgattcatc ctctctacat cacacgctta aataattttt ctaattcgta ttttgaaaaa
    14461 ttaatgataa ttggacgacc atgtggacat gtaaatggat cttccgcttc tcttaattga
    14521 tcaattaaat cagacatttc atgtttttgt aaataatgat tcgctttaat agatttttta
    14581 catgacatca tgattgctac atcttcacgt aattttttga tatctacttt tttctcttcc
    14641 aaaattagct caatcatatc tttaataatt tcttctactt catctttagg gaaccaaact
    14701 ggatagctac ttacaatata atcatgacca ccaaaatgtt ctaacatgat acctacttgt
    14761 tgaagctcat ttttatattg atcaatgact aattgttcat cttttgaaaa atgaaatgtt
    14821 aacgggatta ataaatcttg tatttcattg gtaacttcac ctattttatc tcgaaaatat
    14881 tcatatttta ttctttcttg agctgcatgc tggtcaatca tgtacatgcc aaattcattt
    14941 tgagcaataa tatacgttcc atgtacttgg ccaacaattt ccatatatgg aattcttcgt
    15001 ttagggtctt tactttttgt tcctttaata tcgtttacat cataataatc atcagacgct
    15061 ttattctcat aattttgcac agatgtatcg tcatttgaat caaatgtttg atccatttct
    15121 tgtaaaattt cttgttgttt tttgaagtag tcttcatctt tttcacgtgt attattataa
    15181 ccagaagatt catcatcagc tattaaactt tcagatgttt tcgttacgaa tggattatat
    15241 gaatcttctt ttataactat ctcatcgttt tgattttctg ccataaatgg cttactgtta
    15301 ctttcttcag atgaaaacgt cttctcttgt ttattctctg tgttttgtct ttgttcaaat
    15361 tcgatttttt gttgttcgaa tgaatgtaac actttatttt ttttcggcac ataatccaag
    15421 ttatttttag gaattaatat acggtcttta aatgcttctt gtattttgct cactatcaat
    15481 tgatatagtt gctcttcttt tgataaacgc acttctagtt ttgttggatg aacatttacg
    15541 tctactaaga ttggatccat ttcaatatta atataacaaa tcgggaacct acctattgtt
    15601 aagagtgtat gatagccttc taaaatcgct ttatttaaca taaagttttt aatgtatcgt
    15661 ccattaataa aaatagaaat atagtgctta ttacttctag aatgttcagg ctttgcaaca
    15721 aaaccttcaa tgtgataatc acttgtatct ccagatatat gtactaaatc tcgtgcaact
    15781 ttcatcccat aaatctctgc catcacttca ttagttcttc ctgaaccatt tgtacttaac
    15841 attgttttgc catctgaaat gagtgctatt cgaatgtccg gatgactcat tgccattctg
    15901 ttgacaatat ctgttatttt acctagttca gtgtataaac tttttatata ttttaaacgt
    15961 gctggtgtat tataaaataa tgattctaca agtatatctg ttcctttttt cgcttttgca
    16021 ggcttatgat ttaatatttc accattttct acatatattt catttccatt agcattatcc
    16081 gtgcaagtct tcaatgttac tttagcaact gatgaaatac tggctagtgc ttcaccacgg
    16141 aatcctaacg tcctaatatg aaataaatct tcatcttgat ctaacttact agtcgcatgt
    16201 ctatgaaata ctaatcctaa gtcttccgct tcaattccgc ttccattatc gactacgcga
    16261 atagattgga cgccagattc ctctacttca atgcttattt ctgtagcgcc tgcatctata
    16321 gcattttcca acagttcttt cacaacagaa ctcggtcttt caactacttc acctgctgcg
    16381 attttatttg ctaatgaggt ttggagttct ttaattttcc ccattttgca acacctctat
    16441 tttaattgat tttgtaattc acttaacttc accaatgcct caattggtgt catattagat
    16501 aaattcaaat ttttaatttg tagttcaatc tcgctttctt gatcattttc aaacaaatca
    16561 aatgatgctt gttcaaagtc tttttgagat aaagtatcag ttgtttcttc aacacttaag
    16621 tttgaatttt cttgattaat ttcaggttca ttttcgacca tttttaaatt tgatatcgat
    16681 gattttttac cagcagacgc ttcaaactcg cttaggatca cttgtgctct gctaataact
    16741 ttttcaggta aatcagctaa tttcgcaact tgaataccat aactatcgtc aactgcacca
    16801 tctttgactt tatgcaagaa tataagttca cctttatatt cattagcagc gacgtgaaca
    16861 ttttttagac ttggtaatgc ttgatctaat gttgtcaatt catgataatg tgttgaaaat
    16921 aacgttttag catgtgatgt ttcagctaca tactctatca ttgcctgcgc taaagctaaa
    16981 ccgtcatacg ttgaagtacc acgtccaatt tcatcgaaaa taatcaaact atcctctgtt
    17041 gcataagtta atgccttttg tgcttctagc atttctacca taaacgtact cttacctgaa
    17101 accaaatcat ctgccgcacc tattctagtg aatatttgat caaatatagg taacacagcc
    17161 tctttacaag ggacataagc tcccatttgg gccattatac taattatggc aacttgtctc
    17221 atatacgtcg atttaccaga catattcgga cctgtaatta aatatataaa tgtttcgtta
    17281 tctaatcgac aattattagg cacatagtca ttataatcca ttactctttc cactactggg
    17341 tgcctagatt ccactaattc taatgtttta ttttcactaa atgaaggcct agtataatta
    17401 tatttttgag caatttctgc aaagctctgt aaacaatcta actctgaaat aattttagct
    17461 tgatgttgta aacgttcagt atatttttta acttcttcac gtagctgaac aaataattga
    17521 tattctaatt caatggcttt gtcttccgca cctaaaatga tatcttcttt ttctttaagt
    17581 tcatcagtta taaatcgttc cgcattcgat aacgtttgct tcctcatata accaaattca
    17641 cttggttcaa aattttgcaa gttggcacgt gttatttcta taaaataacc aaacacttta
    17701 ttaaagctta ttttcaatga ttttattcct gtacgttgtc tttcttttgc ttgtagttct
    17761 gctaaccatg tttttccgtt ttttgaagct tcaagatatt catctaattg catattaaaa
    17821 ccaactttga atagtccacc atctttaact gaaattggtg gttcttctac taaactctgt
    17881 tctaatatat caagtaaatc atcaagtggt tctagttgat taacttgtac aagagtatcc
    17941 tgattcatag aatttagtaa tgctttaata ttcggtattt cagaaatgga atgtttaagt
    18001 tgaattaaat ctctcgcatt aacatttccg taactaacac gcccaacaag acgttcaata
    18061 tcatacactt gattaagata tgttcttaag gtgtctcttt ctatgaaatg agcactaaat
    18121 tcatcaacga tatctaatcg tgcttcaatt tgttctttac ttattagtgg tctatctatc
    18181 cattgtttta atcggcgtgc tcccattggt gttttcgttt cgtccatcag ccaaagtagc
    18241 gttccttttt tggattttaa tcgaatgctt tccgttaact caaggtttct cttagcataa
    18301 aaatccattt tcatataatc tatagctgca tattgaacag catcctcaat atgcgataaa
    18361 tcacgttttt gtgtatgatg aatataatct agcaataatt gtgtcgcttg atacattaat
    18421 ttatgttcag tttgattcac actgtagatt tcagatgata acgtttccct gactgttatt
    18481 gtttctgtca ccatattaat ttgtcttttt aaatgatcgg aaatattgtc attgataaca
    18541 acttcgttag ggtttatcgt cgtaatttca tttaataaag tcgcttcatc attaaaatgt
    18601 gtaaccttta attcaccagt agaaacatca cagtaactaa gcgcaatttc aggttgattc
    18661 ataacaaaac ttaaaatata gttattttgt ttatcatcta caccaccttg ctccatcaca
    18721 gttcctggag tcacaattct tactacctca cgtctaacca tacctttcgt ttgtttcgga
    18781 tcttccatct gttcacaaat agctacttta tatccattat taacaagtgt atctatataa
    18841 ctatctgcag aatgatacgg aacaccacac atcggaattg gattttcttt tttagcatct
    18901 cttttagtta aagtaatttc aagtacacgt gatgcctcct tggcatcttc ataaaacatt
    18961 tcatagaaat cacctagtct aaaaaataat aagcaatctt ggtattctga ttttattttt
    19021 aaatattgct gcatcattgg tgtaacatta gacatattat ttcttcacaa cccttgtctc
    19081 tttttaaaat ttgtctttac aatatattcg tttgtaagct ttctaattat taattattta
    19141 acttatacat tttaacatac ttacttttac aaacctattc ataccatata ataacgaagc
    19201 atcttaaatg tataagaaaa cgcctcaaac ctaataaaat gtgtta
//
LOCUS       DAKSQE010000047        19244 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.047, whole genome shotgun sequence.
ACCESSION   DAKSQE010000047 DAKSQE010000000
VERSION     DAKSQE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19244
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.047"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            48..1328
                     /locus_tag="O0V33_001377"
     CDS             48..1328
                     /locus_tag="O0V33_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HCX8976015.1"
                     /translation="MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILV
                     RDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVIT
                     ANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNG
                     TSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVI
                     KLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAA
                     VEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFEL
                     KTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDN
                     QAYAAVIVGLESSPEELITKHGYEVDKVYPVEGV"
     gene            1334..2395
                     /locus_tag="O0V33_001378"
     CDS             1334..2395
                     /locus_tag="O0V33_001378"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCX8976016.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSEILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPLR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLPNDKDSIIDYIKGAL"
     gene            2397..3311
                     /locus_tag="O0V33_001379"
     CDS             2397..3311
                     /locus_tag="O0V33_001379"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCX8976017.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVAAKYSVDLPNLCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVTKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVR
                     ALNRNVVTCHSELVDINVSGVKERIVYQ"
     gene            3369..4172
                     /locus_tag="O0V33_001380"
     CDS             3369..4172
                     /locus_tag="O0V33_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCX8976018.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQDDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYADEITLDHNENGIPHALKKLL"
     gene            complement(4464..4778)
                     /locus_tag="O0V33_001381"
     CDS             complement(4464..4778)
                     /locus_tag="O0V33_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976019.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFTHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
     gene            complement(4753..4857)
                     /locus_tag="O0V33_001382"
     CDS             complement(4753..4857)
                     /locus_tag="O0V33_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976020.1"
                     /translation="MLPFFILDILHIVYNIDNNNFSGVKFNDWTNKFI"
     gene            complement(4997..6451)
                     /locus_tag="O0V33_001383"
     CDS             complement(4997..6451)
                     /locus_tag="O0V33_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCX8976021.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMASIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHAYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPKFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVIATLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
     gene            6655..8172
                     /locus_tag="O0V33_001384"
     CDS             6655..8172
                     /locus_tag="O0V33_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HCX8976022.1"
                     /translation="MSRQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFR
                     TQQGIENLTDEVAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            8263..8412
                     /gene="rpmG"
                     /locus_tag="O0V33_001385"
     CDS             8263..8412
                     /gene="rpmG"
                     /locus_tag="O0V33_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCX8976023.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
     gene            8866..9135
                     /gene="rpsN"
                     /locus_tag="O0V33_001386"
     CDS             8866..9135
                     /gene="rpsN"
                     /locus_tag="O0V33_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCX8976024.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYETLRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            9292..10269
                     /gene="guaC"
                     /locus_tag="O0V33_001387"
     CDS             9292..10269
                     /gene="guaC"
                     /locus_tag="O0V33_001387"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCX8976025.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKTHIPDSFVIAGNVGTPEGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVE
                     HKGSLTDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD"
     gene            10291..11307
                     /locus_tag="O0V33_001388"
     CDS             10291..11307
                     /locus_tag="O0V33_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCX8976026.1"
                     /translation="MKKFIVTVVAFLSIIIIAPITEFKPFIHLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDGDYN
                     NFIMVSYIKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAERKQHQLSTLKYSKQNSETARKHSKDMAKNHYFDHTNLKGQSPFDRLKKDG
                     IAFNSAGENLAYGQVSSIYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTE
                     NYTD"
     gene            complement(11687..12310)
                     /gene="lexA"
                     /locus_tag="O0V33_001389"
     CDS             complement(11687..12310)
                     /gene="lexA"
                     /locus_tag="O0V33_001389"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCX8976027.1"
                     /translation="MRELTKRQSEIYNYIKQVVQTKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
     gene            12453..12686
                     /locus_tag="O0V33_001390"
     CDS             12453..12686
                     /locus_tag="O0V33_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976028.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANVSAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNSEQRDGKVFALIN"
     gene            12822..13061
                     /locus_tag="O0V33_001391"
     CDS             12822..13061
                     /locus_tag="O0V33_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCX8976029.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVTPEKIKEIQQKRDNKN"
     gene            13182..15170
                     /gene="tkt"
                     /locus_tag="O0V33_001392"
     CDS             13182..15170
                     /gene="tkt"
                     /locus_tag="O0V33_001392"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCX8976030.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEDERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFEFGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            15448..15690
                     /locus_tag="O0V33_001393"
     CDS             15448..15690
                     /locus_tag="O0V33_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCX8976031.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            15677..15775
                     /locus_tag="O0V33_001394"
     CDS             15677..15775
                     /locus_tag="O0V33_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976032.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKDKYVY"
     gene            15869..16336
                     /locus_tag="O0V33_001395"
     CDS             15869..16336
                     /locus_tag="O0V33_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HCX8976033.1"
                     /translation="MLYLIFSIIVALFMGTLVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLVIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRVAMLYKYKKLKKTLIN"
     gene            16460..17581
                     /locus_tag="O0V33_001396"
     CDS             16460..17581
                     /locus_tag="O0V33_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCX8976034.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIMISGNHDGKERLNYGASWFENNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEGAINILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFSYNEENNAIEISE
                     KDDMSIIEMFYNHITDKELSDIQSNKIKNILENELRKED"
     gene            17585..>19244
                     /locus_tag="O0V33_001397"
     CDS             17585..>19244
                     /locus_tag="O0V33_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCX8976035.1"
                     /translation="MKPLHLKLNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIF
                     DAMTYALFGKASTEQREENDLRSHFADGKQPMSVTFEFQLNNRIYKVHRQGPYIKEGN
                     TTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFL
                     ISNSREKQGILRTLFDSEKFEAIREILKEEVKKENAQIENRYQQIDLLWQEIESFDDD
                     KIKGLLEVATQQIDKVIENIPLLQARSKEILAFVNESKETAIKDYEIIEKKTLENNIL
                     KDNINQLNKNKIDFVQLKEQQPEIEEIEAKLKLLQDITNLLNYIENREKIETKIANSK
                     KDISETNNKILNLECDKRNIDKEKRMLEENGDLIESKISFIDKTRVLFNDINKYQQSY
                     LNIERLRTEGEQLADELNNLIEGLEKVEDSIGNNESDYEKIIELNNAITNINNEINVI
                     KENEKAKAELDKLLGSKQELENQINEETSTLKNLEIKLDRYDKSKLDLNDKESFISEI
                     KSAVKIGDQCPICGNEIQDLGHHIDFDSIAKRQNEIKEIEANIHT"
BASE COUNT         7139 a         2615 c         3533 g         5957 t
ORIGIN      
        1 caataataca tttgtttttc tcaaaagcac aaacggaggt acataaaatg aaaaaattaa
       61 atatagcatt attaggatta ggtactgtcg gatctggtgt tgttaaaatc atcgaagaga
      121 accgacagca aattcaagat acattaaata aagatattgt cataaagcat attcttgttc
      181 gagataaatc taaaaagaga ccgctaaata ttagtcaata tcatttaact gaagatgtta
      241 atgaaatttt aaatgatgat tcattagata ttatcgttga agtcatggga ggaattgaac
      301 caactgtaga ttggttaaga acagcactta aaaataaaaa acatgttatt accgcaaata
      361 aagatttatt agcagtacat cttaaacttt tagaagattt agcagaagaa aatggtgtag
      421 ctttaaagtt tgaagcgagt gtagcaggtg gtattccgat cgtaaatgcc ataaataatg
      481 gtttgaatgc gaataatatt tcaaaattta tgggaatttt aaatggtacc tctaatttta
      541 ttttatcaaa aatgactaaa gagcaaacga catttgagga agcacttgat gaagcgaaaa
      601 ggcttggttt tgctgaagcg gatccaactg atgatgtaga aggggtagat gcagcgcgta
      661 aagttgtcat tacatcatat ttatcattta accaagtcat taaattaaac gacgttaaac
      721 gaagaggaat tagtggtgta actttaactg atattaatgt agccgatcaa ctggggtata
      781 aaattaaatt gattggtaag ggaatatatg aaaatggcaa agttaatgca tcagtagaac
      841 caacgttaat tgataaaaag catcaattag cagctgtaga ggatgaatat aacgcgattt
      901 atgtcattgg tgatgccgtt ggtgacacga tgttttatgg aaaaggagca ggcagtttag
      961 caacaggtag tgccgttgta agtgatttat tgaatgtagc attattcttt gaatcagatt
     1021 tacacacatt gccaccacat tttgaattaa agacagataa aacaagggaa atgatggatt
     1081 ctgatgcaga aattaatatt aaagaaaaat ccaatttctt tgtagtagtg aatcatgtca
     1141 aaggttcaat tgaaaatttt gaaaatgagt taaaggcaat attaccattt caccgatcat
     1201 taagagttgc aaattacgat aatcaagcat atgccgctgt tatagttgga ttggaatcat
     1261 caccggaaga attaatcact aagcatggat acgaagttga caaagtatac ccagtagaag
     1321 gagtttaatt ataatgagaa gatggcaagg attagtagaa gagtttaaag cacatttacc
     1381 agtaaatgaa aatacaccaa aattaacttt gaatgaggga aatacaccac tcattcattg
     1441 tgaaaatatg tctgaaatac taggtataga tttatatgtg aagtatgaag gtgccaatcc
     1501 gacaggttca tttaaagatc gcggtatggt aatggctgtg acaaaagcaa aagagcaagg
     1561 taagaaaatt gtaatatgcg cttcgactgg aaatacatca gcgtctgcag cagcatatgc
     1621 agcgagagca ggtttaaaag ctatcgtcgt aataccagaa ggtaaaattg cattaggtaa
     1681 attgtcgcaa gcagtaatgt atggtgcaga aatcgtttct attgaaggaa actttgatga
     1741 agctttagaa attgtaaaag aaattgcaaa aagtggcgaa atcgagcttg taaactctgt
     1801 caatccactt agaatcgaag ggcaaaagac aggctcattt gaaattgtac aacaattaga
     1861 cggtgaagca cctgatattt tagcgattcc tgtaggtaat gcaggtaata ttactgcata
     1921 ttggaaaggc tttaaagaat atcatgaagc taaaggatca caattgccga aaatgtttgg
     1981 cttccaagct gaaggtgcat caccaattgt tcaaaataaa gtcattaaaa atcctgaaac
     2041 gattgcaact gctattcgaa ttggtaatcc tgctagttgg gataaagcga ctaatgctct
     2101 taaagaatca aatggattaa tagatagtgt tactgatgat gaaattttag aagcatatca
     2161 gttaatgaca actaaagaag gtgtctttag tgaaccagca agtaatgctt ctattgcagg
     2221 tttaattaaa ttgcacagac aaggtaaatt acctcaaggt aaaaaagtag ttgcgatttt
     2281 aactggtaat gggttaaaag atccagatac tgctatttca ctattagata atccgataaa
     2341 gccattgcca aatgataaag atagcattat cgattatatt aaaggagctt tataacatgt
     2401 cgaatgtttt ggagttaaca attcctgcat caacagccaa ccttggagtt ggctttgatt
     2461 ctataggtat ggctttagat aaatttttgc atctgtctgt aaaagaaaca tcagggacaa
     2521 aatgggaata tattttccat gatgatgcat cgaagcaatt acctactgac gaaacaaact
     2581 ttatttatca tgtggcacaa caagttgctg ctaaatatag tgttgacttg cctaatttat
     2641 gtatcgaaat gagaagtgat attccattgg caagagggtt aggttcgtca gcttctgctt
     2701 tagtaggagc tatatatatc gcaaattatt ttggtgatat ccaactatct aaacacgagg
     2761 tattacaatt agcgactgaa atcgaaggac atcctgataa tgttgcgccg accatttatg
     2821 gtggtttaat cgctggatat tataatgatg tcacgaaaga aacgtcagtt gcacatatcg
     2881 acataccaga cgtggatgtg attgtaacga taccaactta tgaactaaaa acagaagcat
     2941 caagacgtgc tttaccacaa aaattaacac atagtgaagc cgttaaaagt agtgcaatta
     3001 gtaatacaat gatttgtgca ttagcacagc acaattatga attagcaggt aaactcatgc
     3061 aacaagatgg ctttcatgaa ccgtatcgtc agcatttaat tgctgaattt gatgaagtga
     3121 aaacaattgc tagtcaacat aatgcctatg caactgtaat tagtggtgct ggaccaacta
     3181 ttttaatatt tagtcgcaaa gaaaatagtg gcgaattagt tcgcgcttta aatagaaatg
     3241 tagttacatg tcattctgaa ttagtggata ttaatgttag tggtgttaaa gaacgaattg
     3301 tataccaata gacactttat attgtaaaat agtattgaat gtgaaataga gaggagattt
     3361 aatgcgatat gacaaattat aaagttgtcg ttttagacat ggatgacaca ttgctaaatt
     3421 cagataatgt gatatcagaa gaaactgcaa attatttaac agcaattcaa gatgaaggtt
     3481 attatgttgt tttagcatct ggtagaccta ctgaaggtat gattccaact gctagagatt
     3541 taaaattacc tgaacatcat agctatatta ttagttataa cggcagtaaa acgattaaca
     3601 tgactaatga agaagtagaa gtaagtaaat cgattggtaa gcaggatttc gatgaaattg
     3661 tagattattg tcgagataga ggctttttcg ttcttacata tcatgatggt caaattattt
     3721 acgacagcga acatgaatat atgaatattg aagcagaatt aactggttta ccgatgaaac
     3781 gtgttgatga cattaaagcg tatattcaag atgatgttcc taaggtcatg ggtgtagatt
     3841 atgtagcgaa tattacagaa gctagaattg atttgaatgg tgtgttcaat gataatgtag
     3901 atgctacgac aagtaagcca ttcttcttag aatttatggc taaagatgtt tcaaaaggta
     3961 atgcaattaa agcgttatgt cacaaattgg gatattcggt ggatcaagtc attgcttttg
     4021 gtgatagtat gaatgataaa tcaatgtttg aagtcgcagg tctagctatt gctatgggga
     4081 atgcatcaga tgaacttaag caatatgcag atgaaattac gttggaccat aatgaaaatg
     4141 gtattccaca tgcgctcaaa aaattgttat aaattttaaa ataagcctaa gcacaggata
     4201 tttgaataag atatcttgtg tcaaggcttt ttatttatgt gaaaatgcgt tagctataat
     4261 atggcgcatt tgaaatacat attatagatt agtaatgata tctaaagaat agagtagatg
     4321 catatttttt aagtaaatca agtattaatt agtcacggaa ggtagataaa taaatttgaa
     4381 tcattctgag taataacgaa tgtattaaaa aatgcagtga caatctaaca ttaattgtta
     4441 aaatcactgc aagcatcttg attttattta gctgtttgtg tccagtcatg cttccctctg
     4501 aagcgtttta catgagcata tatttggtca gcagtataat ctaaatttgt ataaactgta
     4561 atactgaacg ctgattggtt ttcagcaaat gattcttcgg aaaataattc ctttaagtgc
     4621 gtaaataaat cattcatttg tttatggttt aaaccgggat attcccttaa agttctcttt
     4681 aataattgtc cgttcttttc ttctaatgct tgaactacaa taacaaatct ctcatcttgc
     4741 tttagtacat catcaaataa atttgtttgt ccaatcattg aatttcaccc ctgaaaagtt
     4801 attgttatct atattataca cgatatgcaa aatatctaaa ataaagaatg gtaacatggt
     4861 cataataaaa tataaacaga atactagtta atacacttta tactagaaaa agatattaaa
     4921 gtatatctac tttacaccat taaaaaagcg gcaaatgcta tagataatct aaagcatctg
     4981 ccgtgtggga atttatttat ttttgattgt cataatcgtg tggttttaaa ttaattgttt
     5041 ctagctttac aaattttgtt ttgtgaatga ttttatgaat aaagtaaatc aacgctagaa
     5101 tgattaaagg taaaaagttt ttaaaagcat ttaaccattg atcttttaaa atatattcaa
     5161 ctgaaccacc aaatagcaag aataatagcg tagcgatgac aatgattggt cctaatggat
     5221 aaaaaggtgc tttatatggt aggaccttat taggatcttg accttgtttt ttaatagctt
     5281 gtcgcaatcg tatttgtgac caaatgcttg atccccaaac aactataatc attgaaccaa
     5341 taatttcaag taaattaaaa acggcatttg aattaaagtt tgcataaata ataacaataa
     5401 caacgactgc atatgtagtt aataatgctc ttaatggtaa ttttgttgtc ttgtttaatt
     5461 tacttaaaaa tttaggtgct tttttgtctg aacttaagga atacaacatt ctgcctgttg
     5521 tataaacacc tgaatttgca gcggataata gtgaagttaa aataaccgca ttgattactg
     5581 atgctgcaaa ggctatgcct actctatcga atacaattgt aaatgggctt tgacttattg
     5641 aactacttgc tcttaataat gatggatctg tgtacggaat aattgcacca atgactgcaa
     5701 ttgataatac atagaataaa agaatacgcc aaaacacttg tttaattgct ttaggcatag
     5761 actttttagg atcatctgat tcgccagcag ttactgctac tacttcagta ccaccaactg
     5821 agaatccagc gactaataat acgcctagga aaccagagat accaccaaca aacggtgctt
     5881 ggccttttgt atagttttca aatccatatg catgaccacc taagataccg aaaatcatta
     5941 aaaagccaaa aataacgaat acgatgattg ttaacacttt aattaatgat aaccaaaact
     6001 cagtttctcc aaatgatttt acagaaaaaa tgtttaataa tagtaaaatt gtaataaaga
     6061 ttaagctcca agtaatgggg tggaaaaatt taaatgtgtc ccagaaataa agcacatttg
     6121 acgctactat gacatcaaca cttgtaacta atgaccacaa tgcccaatac aaccatccca
     6181 tggtaaagcc tagagatgag tcaataaagc gtgttgaata agagctgaat gaacctgata
     6241 ctggataaaa tgttgccaac tctccaattg atgccattaa gaaatatagc atgacaccaa
     6301 taacaagata agcgagtata gcgcctccag gaccagcttg agaaatgata ttaccagtag
     6361 ctacaaatag accagtccca attgcaccac ctatagcaat catggaaatg tgtcttgagt
     6421 taagactacg gttcatttta ttatcttcca tatttagtct cccatctatt taaatatacc
     6481 cattattgta agctttttaa gtgtactatt caataactat ttagcactgt atagcggaaa
     6541 aattaaaatt ttctgatttt ttaatcatct tgagcatgtt taattgtaat tctgatgggg
     6601 ttaaattata atatgtatta aattataatt attataaatt gtggagggat gactatgtca
     6661 cgacaagata aaaagttaac tggtgttttt gggcatccag tatcagatcg agaaaatagt
     6721 atgacagcag ggcctagggg acctctttta atgcaagata tttacttctt agagcaaatg
     6781 tctcaatttg atagagaagt aatacctgag cgtcgtatgc atgccaaagg ttctggtgca
     6841 tttgggacat ttactgtaac taaagatata acaaaatata cgaatgctaa aatattctct
     6901 gaaataggta agcaaaccga aatgtttgcc cgtttctcta ctgtagcagg agaacgtggt
     6961 gctgctgatg cggagcgtga cattcgagga tttgcgttaa agttctacac tgaagaagga
     7021 aactgggatt tagtagggaa taacacacca gtattcttct ttagagatcc aaagttattt
     7081 gttagtttaa atcgtgcggt gaaacgagat cctagaacaa atatgagaga tgcacaaaat
     7141 aactgggatt tctggacggg gcttccagaa gcattgcacc aagtaacgat cttaatgtca
     7201 gatagaggga ttcctaaaga tttgcgtcac atgcatgggt tcggttcaca cacatactct
     7261 atgtataatg attctggtga acgtgtttgg gttaaattcc attttagaac gcaacaaggt
     7321 attgaaaact taactgatga agtagctgct gaaattatag caacagatcg cgattcatct
     7381 caacgcgatt tattcgaagc cattgaaaaa ggtgattatc caaaatggac aatgtatatt
     7441 caagtaatga ctgaggaaca agctaaaaac cataaagata atccatttga tttaacaaaa
     7501 gtatggtatc acgatgagta tcctctaatt gaagttggag agtttgaatt aaatagaaat
     7561 ccagataatt actttatgga tgttgaacaa gctgcgtttg caccaactaa tattattcca
     7621 ggattagatt tttctccaga caaaatgctg caagggcgtt tattctcata tggcgatgcg
     7681 caaagatatc gattaggagt taatcattgg cagattcctg taaaccaacc taaaggtgtt
     7741 ggtattgaaa atatttgtcc ttttagtaga gatggtcaaa tgcgcgtagt tgacaataac
     7801 caaggtggag gaacacatta ttatccaaat aaccatggta aatttgattc tcaacctgaa
     7861 tataaaaagc caccattccc aactgatgga tacggctatg aatataatca acgtcaagat
     7921 gatgataatt attttgaaca accaggtaaa ttgtttagat tacaatcaga ggacgctaaa
     7981 gaaagaattt ttacaaatac agcaaatgca atggaaggcg taacggatga tgttaaacga
     8041 cgtcatattc gtcattgtta caaagctgac ccagaatatg gtaaaggtgt tgcaaaagca
     8101 ttaggtattg atataaattc tattgatctt gaaactgaaa atgatgaaac atacgaaaac
     8161 tttgaaaaat aaatttgata tgtagtttct atattgcgta gttgagcagt ttatgatatc
     8221 ataataaatc gtaaagattc ctaacaagag agggtgttta acatgcgcgt aaacgtaaca
     8281 ttagcatgca cagaatgtgg cgatcgtaac tatatcacta ctaaaaataa acgtaataat
     8341 cctgagcgta ttgaaatgaa aaaatattgc ccaagattaa acaaatatac gttacatcgt
     8401 gaaactaagt aattcttatc attcaaatac gacgatttga aaataaagcg ggcttaccta
     8461 ttatatgggg gagctcgctt ttttatgaaa tttttgtgaa gagtgattaa tgaattgagt
     8521 ttcatcgata gaacaatata tgtttgtatt agttgttact ttattaaaat ttgagaatat
     8581 tgatagaagg aaatagatta ctaattttat aaagtcactt tgttagcgaa tgcttgaaag
     8641 agtaattaat atagtagaat ttaaaatttc aatgcgggat ttaataagta agaagtaatt
     8701 gtggatatgt tttataaatg ttcgacaata cactttgatg ttaaatttga tggtttaaaa
     8761 aatgatttaa caaagaaatg aaactttact gttgaattat gtgaggattg tgttattata
     8821 taaatcgtaa taattacgat ttgataaaaa gtgaggtaac tatatatggc taagaaatct
     8881 aaaatagcaa aagagagaaa aagagaagag ttagtaaata aatattacga attacgtaaa
     8941 gagttaaaag caaaaggtga ttacgaaacg ttaagaaaat taccaagaga ttcatcacct
     9001 acacgtttaa ctagaagatg taaagtaact ggaagaccta gaggtgtatt acgtaaattt
     9061 gaaatgtctc gtattgcgtt tagagaacat gcgcacaaag gacaaattcc aggtgttaaa
     9121 aaatcaagtt ggtaaaatca aatttcgtac tttagcccat ttacaatatc aataaaacaa
     9181 tgtacagtat atacgaatgc tataaactga atgttttctc atattaataa ggaaacattc
     9241 ggtttttaat ttgcatttaa aaataattag tttttaaagg ggctatttaa agtgaaaata
     9301 tttgattacg aagatattca attaatacct aataaatgca tagttgaaag taggtctgaa
     9361 tgtgatacaa ctatccaatt tggtccgaaa aaattcaagc tacctgtagt tcctgcaaat
     9421 atgcaaacag ttatgaatga gaaattagcg aaatggtttg ctgaaaatga ttacttttat
     9481 atcatgcatc gttttgatga agaagcaaga atacctttta taaaacatat gcaaaattca
     9541 ggcttatttg catctatttc agttggtgta aagaaagcgg aatttgattt tatagaaaaa
     9601 ttagctcaag aaaagttaat ccccgaatat attacaattg atattgcgca tggtcactca
     9661 gattcagtga taaacatgat taaacatatt aaaacccata tacctgatag ttttgttatt
     9721 gctggtaatg ttggtacgcc agaaggtgtt agagaattag aaaatgctgg tgctgatgct
     9781 accaaagtcg gtataggtcc tggtagagtt tgtattacaa aaattaaaac aggttttggt
     9841 actggtggtt ggcagttagc ggcattaaac atatgtagta aagcagctcg taaacctttg
     9901 attgccgatg gtggtataag aacgcatggc gatatcgcta agtcaattag atttggtgca
     9961 tcaatggtca tgattggttc attatttgcg gcacacgaag aatcacctgg tgaaactgta
    10021 gaacttgatg gtaaacagta taaagaatat tttggtagtg catctgaatt tcaaaaaggc
    10081 gaacataaaa atgttgaagg taaaaaaatg tttgtagaac ataaaggttc gttaacggat
    10141 acgctaaaag aaatgcaaca agatttacaa agttcaatct cttatgccgg tggaaaagac
    10201 ttgaaatcat tacgtactgt agattatgtt attgttagaa actctatttt caacggtgat
    10261 agagattaat atttatagta ggtgatgtaa attaaaaaat tcatagtaac tgttgttgca
    10321 tttttatcaa ttattatcat tgcgccaata acagaattta aaccattcat tcatttacaa
    10381 aatgaagtaa gacaatatat tgacattcac atcaataaag aaacaatttc tgcggaaaat
    10441 aaattggata cgccgaagaa acaacaattt gcctttaaca atatacaaat gaacatgtcg
    10501 aaatcagatg ttgagaaaac attaaataaa ccaaaaagag tgacatttaa tgaatatggt
    10561 acgaagtggt atacgtatta tgatggtgat tacaataatt ttatcatggt aagttacatt
    10621 aaagataaag ttaatgcgtt atatacaaat caaaatatca tcacttcaaa atcaaaaatt
    10681 aaatacaata caccgaaatc ggttgtaagg caaagattag gtgaaccaga aacagagatt
    10741 gttaaaggta gagtgcgtta cgaacaaaat aataaagaat atgatgtttt ccataaaaat
    10801 cacatttata cgacggtatt ttatgataag catcgacgta ataatgtaac agctgtttta
    10861 caagtaagtg atgctatgga aaatagatta aaagaacaat atggagcacc ttcgaaatcg
    10921 cttgcagata gttttgaact acaaaatttt gatttagtta atgctgaaag aaaacaacat
    10981 caattatcta cattgaagta ttctaaacag aattctgaaa ctgctcgtaa gcatagtaaa
    11041 gatatggcca aaaatcatta ttttgatcat acaaatttaa aaggtcaatc accatttgat
    11101 cgattgaaaa aagatggtat tgcatttaac tcagccggag agaatttagc atatggtcaa
    11161 gttagtagta tctatgcaca tcaaggatta atgaattcta ttggtcacag aaaaaatatt
    11221 ttaaatgata cgtttaaaat attaggcgtt ggtgttgatt ttaatgatga aaaacaacct
    11281 ttttggacag aaaattatac tgattaatgt ttttggcatg agattaaagg gtaatgtttg
    11341 tctgtaagga agtagataaa catgatgaca acagataaac caaaagatgc ggatatactt
    11401 gaacgcgtca aagatatact taataaaaaa gaaagaaaaa aataataatg gctcgctctt
    11461 gtaaattatt acgggggcga gttttaattt gtgtaacatt ttgcaggaat ctgtaattca
    11521 aaatttatat taaagaatga gattatctaa caattcgcaa tttctgtttg aactgaattt
    11581 agattagaat aaataaaacc tcttgaaaac gtaacttttt caagaggtcc aaaatttata
    11641 aaaatgaggg cttaacaatt acgataatat ataacggttt aaaatattac atttcgcggt
    11701 acaaaccaat tactttcccg attacagcaa cattgtcgag gtaaattggc tccattgtac
    11761 tattttcagg ttgtaatcga taacgatttt tttctttata gaagcgtttg acagttgctt
    11821 catcttcctc agtcatagca acaataatgt ctccattttc tgctatggtt tgactgcgaa
    11881 caattacttt gtctccgtct aatataccag cctcaatcat actgtcgcct acgacgttta
    11941 atatgaatat gtcgctattg tgtgtcgatg ttaagtgttc aggtaatgga aaatattctt
    12001 caatattttc tactgcggta ataggaacac ctgctgtgac tttaccaata actggcacat
    12061 gaatcgtttc ttccatatta atattatcat ttgtttgatc acttacaatt tctatagcac
    12121 gtggtttcgt tggatctctt cttatatagc ctttttcttc aagacgtgaa aggtgaccat
    12181 gaacagttga actggatgct aagccaactg cttcaccaat ttcgcgaaca ctaggcggat
    12241 aacctttcgt ttgaacaact tgtttaatat agttatatat ttcgctttgt cgttttgtta
    12301 attctctcat atataggcac tccctaaatt aatttaaata cagtatatca ttaattcaca
    12361 accaaaacaa acatttgttc gttaaaatat tgacacagaa cataagttct gataaattac
    12421 taatacaaac aaatgttcta ggagtgaaaa tgatgtttta caataaatat aaaaacgtat
    12481 caacatatat catcatattt ttagtttcaa gtgcagcctt tgcaatattc ttgttaagtg
    12541 cgaacgttag tgctcactcg gaacaagtgt acgaaatgac tgaccatcaa attaagaaca
    12601 atacgataaa taaagcatac gaacataaag accctacaaa caatagcgaa caaagagatg
    12661 ggaaagtgtt cgctttaata aattgataca ttgtcacaac gttattttgc ctatttttgc
    12721 gaaatagcgt tttttattac ttttttgctg atcttaaatt tgttatattt tgttaaagta
    12781 ttataatgat tgaataaaca aattgaaggt aggtttttta attgagtaat tctgatttga
    12841 atatcgaaag aattaacgag ttagctaaaa agaaaaaaga agtaggatta actcaagaag
    12901 aagcaaagga gcaaacagcg ttaagaaaag cttatcttga gagttttaga aaagggttta
    12961 aacaacaaat tgaaaatact aaagtaattg atccagaagg taatgatgta acacctgaaa
    13021 aaattaaaga gatacaacaa aaaagagata ataaaaatta aaacacaaat ctgtaaagaa
    13081 ttttctgaca ttataacttg aaataagtat tttacttatc tttttatttt aaaataagtt
    13141 ataatgtatt tgataaaatt gaagaaggga agatacacaa gatgtttaat gaaaaagatc
    13201 aattagctgt tgatacgcta cgtgcactaa gtatcgacac aatcgaaaaa gcgaattctg
    13261 gtcatccagg attacctatg ggagctgccc caatggctta cactttgtgg acacgtcatc
    13321 tgaattttaa tccacaatct aaagattact tcaatagaga ccgtttcgta ttatctgcag
    13381 ggcatggttc agcactattg tatagcttgt tacatgtttc tggtagttta gaattagaag
    13441 aattaaagca atttagacaa tggggttcta aaacaccagg tcatcctgaa tacagacata
    13501 cagatggtgt agaagttact actggaccac ttggacaagg ttttgctatg tcagttggat
    13561 tggctttagc agaagatcac ctagcaggga aatttaataa agaaggatat aatgttgtag
    13621 atcattacac atatgtatta gcttctgacg gtgatttaat ggaaggtatt tcgcatgaag
    13681 cagcttcatt tgctggacat aataaattaa gtaaattagt tgttttatac gattcaaatg
    13741 atatttcatt agatggcgaa ttaaacaaag ctttttctga aaacacaaaa gctcgttttg
    13801 aagcatatgg ttggaattac ttactagtta aagatggtaa tgatttagaa gaaattgata
    13861 aagcgattac tacagctaaa tctcaagaag gaccaacaat tattgaagtt aaaacaacaa
    13921 tcggatttgg ttcaccgaat aaagcaggaa ctaatggtgt tcatggggca cctttaggtg
    13981 aagatgaaag aaaattaaca ttcgaaaatt acggtttaga tcctgaaaaa cgttttaatg
    14041 tttcagaaga agtatacgaa attttccaaa atactatgtt aaaacgtgct aatgaagatg
    14101 aatctcaatg gaattcatta ttagaaaaat atgcagaaac atatcctgaa ttagcagaag
    14161 aatttaaatt agcgattagt ggtaaattgc ctaaaaatta taaggatgaa ttaccacgtt
    14221 ttgaatttgg tcataatggt gcatctcgtg ctgattctgg tactgttatt caagcaatca
    14281 gtaaaactgt cccttcattc tttggtggat cagcagacct tgctggttca aacaaatcca
    14341 atgtaaatga tgcaacagat tatagttctg aaacacctga aggtaaaaat gtgtggtttg
    14401 gtgtacgtga atttgctatg ggtgctgctg taaatggtat ggctgcacat ggaggtttac
    14461 atccatatgg tgcaacattc ttcgtattta gtgattattt aaaaccagcg ttacgtttat
    14521 catcaattat gggattaaat gcaacgttca tcttcacgca tgattcaatt gcagtaggtg
    14581 aagatggtcc tactcatgaa ccaattgaac aattagctgg attaagagcc attccaaata
    14641 tgaatgttat ccgtcctgct gatggtaatg aaacaagagt agcatgggaa gttgccttag
    14701 aatctgaatc tacacctact tcattagtat tgacacgtca aaacttaccg gttttagatg
    14761 taccagaaga tgtagttgaa gaaggcgttc gaaaaggtgc ctatacagtt tatggctctg
    14821 aagagacacc agaatttcta ttattagctt caggttcaga agttagtctt gcagttgaag
    14881 ctgctaaaga tcttgaaaaa caaggtaaat cagtgcgtgt tgtttcaatg cctaactgga
    14941 atgcatttga acaacaatct gaagaatata aagaatcagt tattccatca agcgtaacaa
    15001 aacgtgttgc gattgaaatg gcttcaccgc ttggatggca taaatatgta ggtactgcag
    15061 gtaaagttat tgctattgac ggctttggcg caagtgcacc tggcgattta gtagttgaaa
    15121 aatatggctt tacaaaagaa aatatcttaa accaagttat gagcttataa gaataattta
    15181 taaagcgagt atgtttagaa gtctaggatg cataatctta ggcttctttt aaagtgttga
    15241 aatttagagt atcgcactca aactacatca taagtgataa gttttgaaaa tatactattt
    15301 cagattaatc tttaaaacct ctgttataac agcatgattt ttgatattat ttttagtatc
    15361 gatattaaaa tacttgaata aactagttct tgaaataatg tgatgaattt agtaaaattc
    15421 agtaggataa agaaagcggg tgaaacaatg gcaacttggt tagcaattat ttttatagta
    15481 gctgcattaa ttttaggttt aattggaggt ttccttttag ctagaaaata tatgatggac
    15541 tacttgaaga aaaacccacc aatcaacgaa gaaatgcttc gtatgatgat gatgcaaatg
    15601 ggtcaaaaac cttctcagaa gaaaattaat caaatgatga cgatgatgaa taaaaatatg
    15661 gatcaaaata tgaagagtgc gaaaaagtaa attcgcaatt gatagaggct attttccaga
    15721 tatggaaatg gcctcttttt ataatcaaat taataaggat aaatatgttt attaaaatta
    15781 aagttaacaa aatgacgaat agactgagaa atgctataat tcattttgta tgatttacag
    15841 agagtttatt taacgagaag gtgtctgcgt gctctattta atattttcaa tcattgtagc
    15901 tttatttatg ggaactctag ttatagttat tcgtatgaaa gctcaaaatt atccggtaaa
    15961 tgagaaaaaa atagttttgc caccgttttt tatggcgact ggtgcattga tgtacgtcgt
    16021 tccatatttt aggctaacag gatcggaaat gctagaagcc tttataattg gtttgctttt
    16081 ttctacagtt ctaatttgga cttctcgatt tgaagtcaaa ggtacagaaa tttatatgaa
    16141 acgatctaaa gcatttccag ttattttgat ttcattactt gtcattcgta ctgtgatgaa
    16201 aatattcatt agtaatgaaa tagatcctgg agaattaggc ggcatgttct ttttattagc
    16261 attctgtatg attgttcctt ggagagtagc aatgctatat aaatacaaaa aactaaagaa
    16321 aacattaatc aattaattac ttctaaaacc acttgtgatc gacttctaaa tcagtcaatg
    16381 agtggtttta attttacttg aaaaggtgaa atgattatat taaaataaaa gcgaacaaat
    16441 gttctatatg gaggcagtaa tgaaaattat acatacagca gactggcact tagggaaaat
    16501 attaaatggc aaacagcttt tagaagatca agcgtatatt ttagatatgt tcgtagaaaa
    16561 aatgaaagaa gaagaacctg atatcattgt gatagctgga gatttatatg acacaacata
    16621 tccaagtaaa gatgcaatca tgttattaga acaagcgatt ggaaagctga atttagaact
    16681 gcgtatacca ataattatga ttagcggaaa tcacgatggt aaagagagat taaactatgg
    16741 ggcgagttgg tttgaaaata atcagttatt tataagaaca gattttacat cgattaattc
    16801 accaatagag ataaatgggg ttaattttta tacactccct tatgctactg tgagcgaaat
    16861 gaaacactat tttgaagatg acaccattga aacgcatcaa caaggaatta cgcgctgtat
    16921 tgaaacaata gcaccggaaa ttgatgaagg tgccatcaat attttaatta gtcatctgac
    16981 tgttcaaggt ggaaagacat ctgattctga aagaccatta actattggaa cggttgaatc
    17041 agttcagaaa ggtgtttttg atatatttga ttatgtcatg ctaggtcact tgcatcatcc
    17101 atttagtata gaagacgaca aaattaaata tagtggctcc ttattgcagt attcattttc
    17161 ggaagcgggt caagcaaaag ggtatagacg tgtaacaatt aatgatggca ttattaacga
    17221 tgtatttatt cctcttaagc cgcttagaca attggaaatt atctcgggcg aatataatga
    17281 tgttattaat gaaaaagttc atgtgaaaaa taaagataat tatttacatt ttaaacttaa
    17341 aaatatgtct catattactg atccaatgat gagtttaaaa caaatttatc ctaatacttt
    17401 agcgctgacg aatgaaactt ttagttacaa tgaagaaaat aatgctatag aaataagtga
    17461 aaaagatgac atgtcaatca tcgaaatgtt ttataaccat ataactgata aagaattatc
    17521 ggatatccaa tctaataaga taaaaaatat tttagaaaac gaattgagaa aggaggatta
    17581 atgaatgaaa ccattacatt taaagttgaa taatttcggc ccctttttaa aagaagaaat
    17641 tgatttttct aaaattgata ataatgaatt gtttttaata agtggtaaga ctggatcggg
    17701 taaaacaatg atttttgatg caatgactta tgccttgttt ggtaaagcat caactgaaca
    17761 aagagaagaa aatgatttga gaagtcattt cgctgatggt aaacagccga tgtcagtaac
    17821 gtttgaattt caattaaata atcgaattta taaagtgcat agacaaggcc cttatatcaa
    17881 agaaggtaat acaacaaaaa cgaacgctaa atttgatgta tttgagatgg tggatggcaa
    17941 gtatgaaatt agagaaagta aagtaatttc aggtacccaa ttcattattg aattattagg
    18001 agtaaatgca gatcaattcc gacaattgtt tattttgcct caaggtgaat tcaaacgctt
    18061 tttaatatca aacagtcgtg aaaagcaagg gatattaaga acgctgtttg acagtgaaaa
    18121 atttgaagct atacgagaaa tattaaaaga agaagtaaaa aaagaaaatg ctcaaatcga
    18181 gaatagatat caacaaattg accttttatg gcaagaaatt gaatcatttg atgatgacaa
    18241 aataaaaggc ttattagagg ttgccactca acagatagac aaagtgattg aaaatatacc
    18301 acttttacaa gctaggtcga aagaaatact agcatttgta aatgaaagta aagaaactgc
    18361 tattaaagac tatgaaataa tagaaaagaa aacactagaa aataatatat taaaagataa
    18421 tattaatcaa ctcaacaaaa ataaaattga tttcgttcaa ttgaaagagc aacaacctga
    18481 aatagaggaa attgaagcta agttaaagtt gttacaagat attacaaacc tattgaatta
    18541 tattgaaaat agagaaaaaa ttgaaactaa aatcgctaat agcaaaaaag atatttctga
    18601 aaccaataat aaaatattga atcttgagtg tgataagcga aacatagaca aagagaaaag
    18661 aatgttagaa gaaaacggag atttaattga aagtaaaatc tcttttattg ataaaactag
    18721 agtattattt aacgatatta ataagtatca acaaagttat ctcaatattg aacgcttgag
    18781 aactgagggt gaacaattag ctgatgaatt aaataatcta attgaaggtt tagaaaaggt
    18841 cgaagattca ataggtaata acgaaagtga ttacgagaaa attatcgaac taaataatgc
    18901 gataactaac ataaataatg aaattaatgt aattaaagaa aatgaaaaag ctaaagctga
    18961 attagataaa ctattaggta gtaagcaaga gttagagaat caaattaatg aagaaacatc
    19021 tacattgaag aatctcgaaa taaaattaga tcgctacgat aaatcaaaat tggacttaaa
    19081 tgataaagaa agttttataa gtgaaattaa atctgctgta aagattggag atcaatgtcc
    19141 gatatgtggt aatgaaattc aggatttagg gcatcatatt gattttgaca gtatcgctaa
    19201 acgtcaaaat gaaattaaag aaattgaagc aaatatccac acta
//
LOCUS       DAKSQE010000048        18988 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.048, whole genome shotgun sequence.
ACCESSION   DAKSQE010000048 DAKSQE010000000
VERSION     DAKSQE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18988
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.048"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            100..491
                     /locus_tag="O0V33_001398"
                     /pseudo
     CDS             100..491
                     /locus_tag="O0V33_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
     gene            625..1023
                     /locus_tag="O0V33_001399"
     CDS             625..1023
                     /locus_tag="O0V33_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCX8976036.1"
                     /translation="MEKSIKIMTIIGIVVQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLFLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLVSGIKLLTNNKPKDEISDLS"
     gene            complement(1273..2097)
                     /locus_tag="O0V33_001400"
     CDS             complement(1273..2097)
                     /locus_tag="O0V33_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCX8976037.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(2348..3655)
                     /gene="brnQ"
                     /locus_tag="O0V33_001401"
     CDS             complement(2348..3655)
                     /gene="brnQ"
                     /locus_tag="O0V33_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCX8976038.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
                     AVIFNPESVLSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRVFGSFGNLV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALVFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKWFMMLPLADIDLAWLVPFMLFAIIGFIIDVFIRRPKQATT"
     gene            4239..5129
                     /locus_tag="O0V33_001402"
     CDS             4239..5129
                     /locus_tag="O0V33_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HCX8976039.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKANLGNQNIMAVAWYQNSAEAKALYLQGYNSAKTQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKGVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQIVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEIK"
     gene            5378..6427
                     /locus_tag="O0V33_001403"
     CDS             5378..6427
                     /locus_tag="O0V33_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HCX8976040.1"
                     /translation="MFLAWNEIRRKKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASPVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTLILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            6440..7117
                     /locus_tag="O0V33_001404"
     CDS             6440..7117
                     /locus_tag="O0V33_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8976041.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIVALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKKRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
     gene            7325..8356
                     /locus_tag="O0V33_001405"
     CDS             7325..8356
                     /locus_tag="O0V33_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCX8976042.1"
                     /translation="MDLLIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPMTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
     gene            8699..9817
                     /locus_tag="O0V33_001406"
     CDS             8699..9817
                     /locus_tag="O0V33_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCX8976043.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKDLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            9792..10715
                     /locus_tag="O0V33_001407"
     CDS             9792..10715
                     /locus_tag="O0V33_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCX8976044.1"
                     /translation="MANLQKYIEYSREVQQARENNQPIVALESTIISHGMPYPQNVEM
                     VTTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVVAMK
                     RIGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAVLGAKIAVAVNKLL"
     gene            10726..11946
                     /locus_tag="O0V33_001408"
     CDS             10726..11946
                     /locus_tag="O0V33_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCX8976045.1"
                     /translation="MNILFAITGIAFALFVAFLFSYDRKNIDFKKTLIMIFIQVLIVI
                     FMMNTTIGLTILTALGSFFEGLINVSKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPKL
                     SRAKLYTIATSGMSAVSMTMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     TDVEIDNLTKSTETKTLNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIMFGSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDF
                     KNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVASFAMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(12051..13583)
                     /locus_tag="O0V33_001409"
     CDS             complement(12051..13583)
                     /locus_tag="O0V33_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HCX8976046.1"
                     /translation="MKEVGFGTLNWVAVIIYLLAMLLIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVMNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYAHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARFIIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTAT
                     KK"
     gene            complement(13623..14504)
                     /locus_tag="O0V33_001410"
     CDS             complement(13623..14504)
                     /locus_tag="O0V33_001410"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCX8976047.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            14661..15521
                     /locus_tag="O0V33_001411"
     CDS             14661..15521
                     /locus_tag="O0V33_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCX8976048.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQIPTPDNKSELITDKV
                     YEIVTGYMKQYHLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIDGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            complement(15798..16598)
                     /locus_tag="O0V33_001412"
     CDS             complement(15798..16598)
                     /locus_tag="O0V33_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCX8976049.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            16738..17406
                     /locus_tag="O0V33_001413"
     CDS             16738..17406
                     /locus_tag="O0V33_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCX8976050.1"
                     /translation="MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYDHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     IMED"
     gene            complement(17532..18845)
                     /locus_tag="O0V33_001414"
     CDS             complement(17532..18845)
                     /locus_tag="O0V33_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCX8976051.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KFGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAIDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIII
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPITNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQAVLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
BASE COUNT         6711 a         2890 c         3507 g         5880 t
ORIGIN      
        1 gataacaatt ggctaaacat tgttcaaaca tcacaatgat aaagcatatt atcagtattg
       61 tagtgtgtgg aaaatgacag ccatctaagg agaaaaatga tgaaaagaat attggtagta
      121 tttttaatgt tagcaattgt attagcaggt tgttctaata aaggtgaaaa gtatcaaaaa
      181 agatattgat aaagtgtaca aagaacagaa tcaaatgaat aaaattgcct cgaaagtaca
      241 aaacactatt aaaacagaca ttaaacaaga agacagtaat acacatgtct ataaaaatgg
      301 taaagtcatt gttattggta ttcaattgta taaagatcgt gaaaaaaatg tattatttcg
      361 catatgaaat aaaagatggt aaggcagaga ttaatagaga aatagaccca attaagtata
      421 tgaaagacca taaagcagat tatgaagatg aaaatgtaga agtggaaaaa aataacgtat
      481 gctttcttta aaacttttaa ccagtttcaa aaaataaatg agctttagcg ataataattt
      541 ttacaataaa cacgatataa atttaaatat ttatatataa gaacacaaac gtaagtttgt
      601 aaataataat aaaggagtaa ggcaatggaa aaatcgatca aaataatgac aataatagga
      661 attgttgttc aaggtttagc aacggtattt agtttactat tgatggtttt agcagcatca
      721 ggtgtaatga ctacagatgt gtcaacaaca gttaatggtg aggttgaccc agttgatgca
      781 gaaacagcag cagcaatttt cactgtatta ttcttattcc tattcatatt tggaatcatt
      841 tcaattattt taggtgcaat cggtatgttt aaagcatcta aaaacaaaaa aatgagtgga
      901 atattgttga ttattggagc tgtaataagt ggtaacataa ttacatttgc tttatggtta
      961 gtcagtggta ttaaactcct tactaataac aagcctaaag atgaaataag cgacttatca
     1021 taaacatcgt atattgaaat ttcaagattt cttaagtaat tatatttctg ttagttttta
     1081 ataaattaac caattaaata aattgctctc ttagaagtag attttcagtc aataactgct
     1141 tttaagagag cattttgtat ttgataatgc tcatagcatc ggacaaaaac cattgacgaa
     1201 ctttaaaaaa atacgttaga caatgcactt ctaaatatta aaggcatcga ctaacgcatt
     1261 gttcaaatag agttaattat ttttataaaa ttgatgatga tcattcaagt aagcatagaa
     1321 taaacctata atgagaccgc ctccgatata gttaccgata aaagcggcag caatatttga
     1381 aatagcagga atgaagtgca atgtatcaac ttgataaatt aagcctccca taaacaagca
     1441 actgttgtaa acgacatgtt cataacccat aaaggcgaat atggtaacac cgaacatcat
     1501 gacaaacatt tttgcaagta catcgtcaat ttgcatggcg ataactaatg aaatattaat
     1561 aaaaaagttg gcgaatatcg ctttcattaa tatacttaca aacccagtag acaacgtttt
     1621 atgttctata actgctgata attgatttaa catatctggc gtcattacat ttgaaaaacg
     1681 catgaaacta aataaaatag cagcacctaa aatatttcct gcaaagcaca ataaaaatat
     1741 tttcaatact ctagtcggtt taattacttt ataatacagg cctacagtaa agtacatgaa
     1801 gttactggtt agtagttcgg agtttgtaaa taaaatgagt actaacgcaa aactgaatgt
     1861 aatggcgctg gccatattca caatgcctgg cggtaaatct ggttcgtgtg ttgctttaac
     1921 tgataatacg aagaccgtaa taatcccgat aataaatcct gccatcatcg cgcgtaatag
     1981 ataacgtttt aaataaacgc tttgtaatat atctttcgtt cttatcgttt cgactacgtt
     2041 atttacccag tcgtccccat aaaatatttt atcccattta atatgtttct ccttcacaat
     2101 gacatcctcg cttttcattt gttaatcaga gtatactatg aatcgcctta tttgtgaata
     2161 atttcacaaa attaatttca caaaattaat tttaaaaact aatatttttg aataacagct
     2221 aggatttttt aacaaatcat ttattttaaa ataattaata gacagacggt atcactgaaa
     2281 agtcatgaat ttcatataaa aaagcctagt aacgatgggt caatcattac taggcagtga
     2341 gcatttatta agttgtcgct tgtttcggac gacgtataaa tacatcgatg atgaaaccga
     2401 taatagcaaa gagcatgaat ggtacaagcc aagctaaatc gatatctgct aaaggtaaca
     2461 tcataaacca tttcaaaata acaccgtgta ataagttgaa actatttagt atttgtaaaa
     2521 ttgaaataat caatgtaata acagttgcga gtcgataggc ccaactgaat ctgaatgtgc
     2581 taaacatgtt agcaaatgat atcagtacaa gtgcaataga cacgggatat attaatgtca
     2641 acaatggtac agcaattttt aaaatcattt ctaaaccaag tgttgtaaat aaaaatccta
     2701 tgatggagaa aacaagtgcg aatattttat aagaaaactt aggtacatgt ttcttagtaa
     2761 atgttgcaca agcgttgacg agtcctatac atgttgttag gcatgcaaga ataaccgtca
     2821 ttccaaatac gaggttaccg aacgaaccaa atactcgtaa tgagttgtac gtcaatatat
     2881 ctgtaccatc tttaaagttt cctggagctg ttgatgcccc aacgtatgca agtgcaaagt
     2941 aaatcattcc aagtaatatg gctgcaataa gacctgaaaa gcagacatat tttaaaattt
     3001 tcatgcgatc tgtgaggcct ttaaacttat agccattgac aatgactacg gaaaaagcta
     3061 acgcagcaac aagatccatt gtaaaatagc cttccaaact tcctgaaatg aaaggatgtg
     3121 ttatatattt atccttaggt gcacttagta cagattcagg gttgaaaatg acagcaatac
     3181 ttaatagagc gaccattaat agtaataacg gtgttaataa tttacctaaa ttatcaacga
     3241 ttttcgatgg atttaaacta atccagtaaa cgatagcaaa aaagattgct gcgaatataa
     3301 ttaaagtcca ttggttgtgc acaggtaaaa tgtgtcttgt accaatttcg tacgcgacat
     3361 ttgcagcacg tggaataccg taaaatgctc cgatagacat gtaaatcacg acagcaaaaa
     3421 taaacccgaa ccatggatgt atacgattgc ctacactttc aacaccttca tcataaaatg
     3481 caacaacaat aacagtaata aaggggagta atatgcctgt aagagcaaag cctagcatac
     3541 caatccacat attttgaccc gctgtatggc cgagcatggg cgggaatatt aaatttccgg
     3601 ctccaaaaaa tagtgaaaat aacatgaggc ccgaaataat aacttgtttt ttcaatgtaa
     3661 tttccttcct aaaactagat attcataata atttaaaaaa tctgaaaaat aaaaacgttc
     3721 ttactttatc tttaaatgac aatactatga ttctattatt ttataaaaaa gattgcaata
     3781 ataacgtctt gctattattg gataatgaca attaaagtgt cgtttttatg aattaaaata
     3841 ggtgaaagta agttggaaat acgttggagc gttgtgattt ttatgagaaa ttgtatgagt
     3901 gaggactgct atttataaaa ggtaaactat ggatgagttt atggttgttc caataggtaa
     3961 acaagagata gcacactgta cacataatga tacgtggatg agtgaatatg actgatagga
     4021 aggcctagtc actcaaatga attaaagttt attacgtgat aaatcacaaa tctctctcat
     4081 gtgataggtc tcccattaaa tcatgcttat atgaaatgtt cacatatttg ttagcttttc
     4141 aagaaataat attaaatatc ttttcatagt atagcaatat taaataaatg catttataat
     4201 tttaactgta ttttaaatat taatcatgag gtgataagat gaataaaatt tcaaagtata
     4261 ttgcaatagc atcattatcg gtagcggtta cagtttcggc accacaaacg acaaattcta
     4321 cagcgtttgc caaaagttct gctgaagttc aacaaacaca acaagcttct ataccagcat
     4381 cacaaaaggc gaatcttggt aatcaaaata ttatggcagt ggcttggtat caaaattcag
     4441 ctgaagcaaa agcattatat ttacaaggtt ataacagtgc aaagactcag ttagataaag
     4501 agattaaaaa gaataagggt aaacataagt tagctattgc tttggattta gatgaaacag
     4561 ttttagataa ttctccatat caaggctatg catcaataca caataaacct ttcccagaag
     4621 gttggcatga atgggtacaa gctgctaaag ctaaacctgt ctatggcgca aaagaattct
     4681 tgaaatatgc tgacaaaaaa ggtgtcgata tctactatat ttctgataga gataaagaaa
     4741 aagatttaaa ggcaacacaa aagaacttaa aacaacaagg tatccctcaa gctaagaaga
     4801 gtcatatttt actaaaaggt aaagatgata agagtaaaga atcacgcaga caaatagttc
     4861 aaaaggatca taaacttgtc atgctatttg gagataattt attagacttt acagatccaa
     4921 aagaagctac agctgaatct cgtgaagcat taattgaaaa acataaagac gatttcggta
     4981 agaaatatat cattttccct aacccaatgt atggtagttg ggaagctacg atttacaaca
     5041 ataactataa agcaagtgac aaagcaaaag ataaattacg taaaaatgct attaagcaat
     5101 tcgatcctaa aacaggcgaa attaaataat atatgaattg gacgtctaca tgtacttaaa
     5161 ggtatatgta ggcgttttta aattgatcct taattttaaa tgtaccgtac aaaaataata
     5221 gacaattaat tgcattttcc ataaatttgc ttttaatata aaaagttcgg gtataatgaa
     5281 ggataagtgt atgtataaca ttacagagcg caacacgacg tacgtgcatc taagaatata
     5341 aacaatgttt aaatcatagt agtagggaga gaaatgcatg tttttagctt ggaatgaaat
     5401 acggcgcaaa aaattgaagt ttggactaat tattggtgtg ttaacgatga ttagttactt
     5461 gctattttta ttatctggat tggcgaatgg tcttatcaat atgaataaag aaggcattga
     5521 taagtggcaa gcagatgcca ttgttctaaa taaagatgcc aatcaaactg tgcaacaatc
     5581 tgtttttaac aagaaagata ttgaaaataa atacaagaaa caagctacat tgaagcaaac
     5641 aggagaaata gtttctaatg gtcatcaaaa agacaatgtc ttagtgttcg gtgttgaaaa
     5701 gtcatcattt ttagttccga gtttaataga agggcataaa gcgactaaag ataatgaagt
     5761 gttagctgat gaaacactta aaaataaagg atttaaaatt ggcgacacat tatcactatc
     5821 tcaatcagat gaaaaattgc atatcgtagg ttttacagaa agtgcaaaat ataatgcgtc
     5881 accagtcatt ttcacgaatg acgctaccat tgccaaaatc aatcctagat tgactggaga
     5941 taaaattaat gcagttgttg tacgtgatac aaattggaaa gacaaaaaat taaaccaaga
     6001 gcttgaagcg gtaagtatta atgactttat tgaaaattta ccaggttata aaccacagaa
     6061 cttaacatta aactttatga tttcattctt atttgtcatt tcagctacag ttataggtat
     6121 attcttatat gtcatgacat tacaaaaaac aagtttattt ggcatattaa aggctcaagg
     6181 atttacgaat ggctatttag cgaacgtagt tatttcacaa acgctcatat tagcattatt
     6241 tggtacggca tttggcttac tgttaacagg cgttacaggt gcatttttac ctgatgcagt
     6301 acctgtcaaa ttcgatgtac taacattgct cgtatttgca attgtgttaa tgattgtctc
     6361 tgtattagga agtttattct ccattttaac aattagaaaa atagatccgt taaaggcgat
     6421 tgggtaggag gtgtagcaaa tgttgaaatt tgaaaatgta acaaagtcat ttaaagatgg
     6481 gaatcgtaac attgaagcgg ttaaagatac aaattttgag ataaataaag gtgatattgt
     6541 agcattagtt ggaccttctg gctctggtaa aagtacattt ctaactatgg caggtgcttt
     6601 acaaacaccg acatctgggc acattttaat caataaccaa gatattacga caatgaagca
     6661 aaaagcattg gcaaaagtta gaatgtctga gataggtttt attttacaag ctacaaatct
     6721 tgtaccattt ttaacggtaa agcaacaatt tacattattg aaaaagaaaa ataagaatgt
     6781 tatgtctaat gaagactatc agcaacttat gtcacaatta ggtctaacat cattgcttaa
     6841 taagttacct tcagaaattt caggtggtca gaaacaacgt gtggcgatag ccaaagcgtt
     6901 atatacgaat ccgtcgatta ttttagcgga tgaacctacc gcggcgttag atactgaaaa
     6961 tgcgattgaa gtcattaaaa ttctacgtga tcaagccaaa aaaagaaaga aagcatgtat
     7021 tattgttaca catgatgaac gacttaaagc atattgtgat cgttcatatc atatgaaaga
     7081 tggcgtcctt aatcttgaaa atgaaacagt agaatagttt tattaagccg gtacatcatg
     7141 tgccggtatt tttatgttta tgtattattt gaataaactt tcacattcaa ttaataataa
     7201 ttattatcga aaattagaaa tattccgtga aatataatat tttttgtagt aaaatggcct
     7261 ctaagtattc aatatttaaa tatggggatt gaatataaaa ttatcgtaat gggggtcaat
     7321 ggttatggat ttattaatag gtactttatt tttatttttg gtcttagtga tttttacatt
     7381 atttacatat aaagcaccta atggtatgcg cgccatggga gcattagcaa atgcagcaat
     7441 cgcaacattt ttagtggaag catttaataa atatgttggt ggtgaagtgt tcggtattaa
     7501 atttttagaa gagctaggag acgctgcggg aggtctaggt ggtgtcgctg ccgctggatt
     7561 aacagcatta gctatcggtg tgtcaccagt atatgcatta gttatagcag ccgcgtgcgg
     7621 tggtatggat ttattaccag gtttctttgc gggttatatg attggatatg tgatgaaata
     7681 tacagagaaa tatgtgccgg atggtgtcga cttaattgga tcgattgtca tcttagcgcc
     7741 attagctcgt cttattgcag tattattaac gccagtagtg aatagtacat tgattcgaat
     7801 tggtgatatt atccaaagta gtacgaatac gaatccaatt atcatgggta tcattttagg
     7861 tggtattatt acggttgtcg gcacagcgcc attgagttca atggcattga cagcattatt
     7921 aggtttaacg ggtgtaccta tggctattgg tgccatggca gcatttagtt cggcatttat
     7981 gaatgggacg ctattccatc gcttaaaatt aggtgatcgt aagtctacga ttgcagtaag
     8041 tattgaacct ttatcacaag cagatattgt atcagccaat ccaattccaa tctatattac
     8101 aaatttcttt ggtggtgcga ttgctggttt aattattgct atgtcaggtt taattaacga
     8161 tgcgacaggt acagctacac cgattgcagg atttttagta atgtttggat ttaatcatcc
     8221 aatgactatt gtaatttatg gtgtagtaat ggcgattgta ggtgcgcttg caggttatct
     8281 tggttcaatc gtatttaaaa aatatccaat tgttactaag caagacatga ttaatcgagg
     8341 tgcagttgac gcatagcatc atcatattga atagtaaaaa caaataaaac atagtaacgt
     8401 gattcagtcg atgaaaaaat cgataatgag tcacgttttt ttatagaaaa atataagaca
     8461 taaaaatgtc ataatttata gtcgacaaat atcacgccac ataaacactt atcattttcg
     8521 taggtgcctt ttactttatg gaatgaactt actttttacg aaattatgcg tattttataa
     8581 acaaatatca ttgatataac ggtaaatgta agcgtttaca acagaaataa ctgcatgcta
     8641 cgatattttt gtaaattcac tgattcaagt attttaagtc aatatgagga gggatgttat
     8701 gagcgattct gagaaagaaa ttttaaaaag aattaaagat aatccgttta tttcacaacg
     8761 tgaacttgct gaggcaattg gattatctag acccagcgta gcaaacatta tttcaggatt
     8821 aatacaaaag gaatatgtta tgggaaaggc atatgtttta aatgaagatt atcctattgt
     8881 ttgtattggc gcagcgaatg tagatcgtaa gttttatgtg cataaagatt tagttgcaga
     8941 aacatcaaat cctgtaacgt caacacgctc tattggtggc gtagcaagaa atattgctga
     9001 gaacttaggt aggcttggcg aaacggtcgc ttttttatct gctagtggac aagatagtga
     9061 atgggaaatg attaaacgat tgtccacacc atttatgaat ttggatcatg ttcaacaatt
     9121 tgaaaatgcg agtacaggtt catatacagc tttaattagt aaagaaggcg acatgacata
     9181 tggcttagca gatatggaag tgtttgacta cattacgcct gaatttttaa ttaagcgttc
     9241 acacttattg aaaaaggcta agtgcattat tgtcgatttg aatttaggca aagaggcatt
     9301 aaacttctta tgtgcctata ccacgaaaca tcaaatcaaa ttagttatca ccacggtttc
     9361 ttccccaaaa atgaaaaata tgcctgattc attacatgct attgattgga ttatcacgaa
     9421 taaagatgaa acagaaacat acttaaattt aaaaatagaa tctactgatg atttaaaaat
     9481 agctgctaaa cgctggaatg atttaggtgt taaaaatgtt attgtgacaa atggcgtgaa
     9541 agaactcatt tatcgaagtg gtgaggaaga aatcatcaag tcagttatgc catcaaatag
     9601 tgtgaaagat gttacaggtg caggtgattc attctgtgct gcagtagtgt atagctggtt
     9661 aaatgggatg tctactgaag atatattaat tgctggtatg gttaatgcaa agaaaacgat
     9721 agaaacgaaa tatacagtta ggcaaaacct agatcaacag caactttatc acgatatgga
     9781 ggattataaa aatggcaaat ttacaaaagt atattgagta ttctcgagaa gttcagcaag
     9841 cacgggagaa caatcaaccg attgtagcat tagaatcaac aattatttcg catggtatgc
     9901 cgtacccaca aaatgttgaa atggtaacaa cagtagagca aattatcagg aataatggtg
     9961 ccattccagc aaccatagcc attatagatg gcaaaattaa aattggttta gaaagcgaag
    10021 atttagaaat actggcaact agtaaagacg ttgctaaagt atctagaagg gatttagcag
    10081 aagttgttgc gatgaagcgt attggtgcta ctactgtagc gacgacgatg atatgtgctg
    10141 caatggctgg tattcaattt tttgttactg gaggtattgg gggtgtccat aaaggtgcag
    10201 aacatacgat ggacatttca gcagacttag aagaattgtc taaaacaaat gtcactgtta
    10261 tctgtgcagg tgccaaatca attttagact tacctaagac gatggaatat ttagaaacaa
    10321 aaggcgttcc agttattgga tatcaaacga atgaattgcc agcattcttc actcgcgaaa
    10381 gcggtgttaa gttaacaagt tcggttgaaa cgccagaacg acttgctgac attcatttaa
    10441 caaaacagca gttaaatctt gaaggtggca ttgttgttgc taatccaatt ccatatgagc
    10501 atgccttatc aaaagcatat attgaggcaa tcataaatga agctgttgtt gaagccgaaa
    10561 atcaaggtat taaaggtaag gacgccacac cgttcttgtt agggaaaatt gtagaaaaaa
    10621 cgaatggtaa aagtttagca gcaaatataa aacttgttga aaacaatgcg gtgttgggtg
    10681 ctaaaattgc tgtcgctgtt aataaattat tgtaggtgat gatacatgaa tattttattc
    10741 gctatcacag ggatagcatt tgcactattt gttgcgtttt tattcagtta tgatcgtaaa
    10801 aacatagact tcaaaaagac gttaataatg atatttattc aagtgttgat tgtgatattt
    10861 atgatgaaca caacgattgg tttaacaatc ttaactgcat taggttcttt ttttgaaggg
    10921 ttaataaatg ttagtaaagc aggtataaac tttgtttttg gagatataca aaataaaaat
    10981 ggctttacat tctttttaaa tgtgttgctg ccattagtgt tcatttctgt attaataggc
    11041 atttttaatt atattaaggt attaccattt attatcaaat atgtaggtat tgctattaat
    11101 aaaataacga gaatggggcg tttagaaagt tactttgcta tttcgacagc gatgtttgga
    11161 cagccagaag tgtatttaac tataaaagat attataccaa agttgtctag agcaaaatta
    11221 tatacaattg cgacatctgg tatgagtgct gttagtatga caatgttggg ttcatatatg
    11281 cagatgattg agcctaagtt cgtagttaca gcagtaatgt taaatatttt tagtgcgctt
    11341 atcatcgcca gtgtaatcaa tccctataaa tctgatgata ctgatgttga aattgataac
    11401 ttaacgaaat ccacagaaac taaaacattg aatggaaaaa cagggaaacc taagaaagtt
    11461 gcctttttcc aaatgattgg tgatagtgcg atggatgggt ttaaaatcgc tgttgtagta
    11521 gccgtaatgt tgttagcatt tatttcatta atggaagcaa ttaatatcat gtttggtagt
    11581 gttggtttga actttaaaca gcttattggc tatgtgtttg caccaatcgc attcttaatg
    11641 gggattccat ggagcgaagc tgttccagct ggctctttaa tggcgaccaa attaattaca
    11701 aatgagtttg tagcaatgct tgattttaaa aatgtcctgg gtgatgtatc tgctcgaaca
    11761 caaggtatca tttcagttta cttagtaagc ttcgctaatt ttggtacggt tggtatcatc
    11821 gtaggttcaa ttaaaggcat tagtgataaa caaggagaaa aagttgcatc ctttgcaatg
    11881 aggctgttac ttggttcaac tctagcttca atcatttcag gatcaatcat tggcctagta
    11941 ttataaatta atcgaagtat ctaaattaaa ttcatggcaa agctaaaccc catcaccaag
    12001 gtggcgcaac agcgcatcat aacttagtga cggggtttta tcataacaat ctactttttc
    12061 gtagccgttt ttgaaatgta tgttgatggt ttatcttttt caaaaattgt taatcccgtt
    12121 atatcttttt tatgttttga agggacaatg aagctaagta tataagcaaa gacaaaagca
    12181 actgtaaatg aaatggtaga tacatagaaa ggtgagttac ctttgccaac accattatag
    12241 acataagcaa agatgatacc caatattaat ccacaaataa caccgaatgt attcgtacgt
    12301 ttagtgaaaa taccaactgc aaatacacca gccaatggaa cgccgaataa tccagttaca
    12361 aacaagaata aatcccataa gtcatttgaa ttagaagcaa ttaagtatag tgacattcca
    12421 aaaccgaaaa tacctgcaat gatgataatg aaacgtgcaa agttaacttc gtgtcgttcg
    12481 ctaccttttc cgaagaagcg ttgcttaatg tcgattgaaa tacaagcaga tatagaattt
    12541 aaactagatg aaatggtaga ctgtgcagct gcgaaaatgg ctgcaataag taatcctgct
    12601 acaaatggtg gcatctcagt caaaatgaaa tatggcacta cagatgatgt attgaagcct
    12661 tttggtaaaa cagcttcatg tgcataaaat gaatacaaca ttgtacccat accataaaat
    12721 aagggtgctg aaattaaagc taggatacca tttgtccata acgatttatt tgtttctttt
    12781 aaactatcag aagcttgata acgctgcacg acgtcttgac tcgccgtgta ttgatacaaa
    12841 ttgttgaaaa tatttcctag gaaaataatt ggaatggcag ctgctgcagt attcagtttc
    12901 caattgtctg cactaattaa ttttttgtgc tcaatcgcat ctgcaaagac agtgccaaaa
    12961 ccgcctttaa tgttcataac accaagaatg atgataacta aagcgccgcc taatagaatg
    13021 acgccttgaa tgaaatcact ccaaaccaca ccttcgaaac cacctaaaaa tgtatataaa
    13081 atacatagta aaccaacgag tgatgcaacg atataagggt tcatgtctga tacagatgta
    13141 atcgctaatg ttggtaagta gataacgatt gctacacgcc ctagatgata aacgacaaat
    13201 aataatgagc caataacacg aatgctaggt ccgaaacgtg cttctagata ttcatatgca
    13261 gatgttacct ttaacttttt aaagaatgga acatagaaat aaatgagtaa tggaataatt
    13321 gctacgatag cgatgttacc tgcaatatat gaccaatctg ttaaaaatgc tttctctggt
    13381 gtagacataa acgtaattgc acttaatgta gtggcataaa ttgaaaaccc aactacccaa
    13441 gatggcaagc gaccacttgc agtaaagaaa ctattcgtac tttggctcgc gcgcttagta
    13501 aaataaacgc caatgagtaa catagctagt agatatatga taacggcaac ccagtttagt
    13561 gtgccaaacc caacttcttt catgggcaac atccccttta caatgtattg attctttgat
    13621 gtctataaat cgtattttgc aatgagttga tctaatgttt gtcgatgtgc ttcgttaaaa
    13681 ggtttgaatg gacgtttcgg taatcctgca tcaatgccac ggtgacgtag tatttctttc
    13741 aatgttggat aaatccccat tgataacact gtttcgatga tgtcgtttga atcatgttgt
    13801 agttggtaag cttcttgaat ttgaccttgt cttgctaagt cgaagatttt tctagcgcgg
    13861 cgaccattaa cgttatatgt agaaccaatt gcaccatcta cgccagaaat cgtagcttga
    13921 actaacattt catcaaagcc agataagatt aatttgtctg ggaatgcttt tctaatacgt
    13981 tcgagtagga agaagtttgg cgctgtatat ttaacaccaa caattttttc gtgattaaaa
    14041 agctcgctga attgttcaat agaaatattc acacctgtta aatctggtat tgcataaata
    14101 atcatattgt tctgtgttgc ttcgataata tcgaaatagt aatctctaat ttcttcaaaa
    14161 gtaaatggat agtagaatgg tgttacggca gaaagtgcat cataaccgag ttctgtggca
    14221 tattttccga gttcaatggc ttcatttaaa tctaacgaac ctacttgagc aatcaatttc
    14281 actttatccc caactgcctc tttggcaact ttgaaaactt gcttcttctg ttctgtattt
    14341 aataaaaagt tttcgcctga gctaccattt acataaagac cgtctaattc ttcagtttca
    14401 atggcatttt gagcaatttg tttaagtcct tgttcattta cttgaccatt ttcatcaaaa
    14461 ggaacgagta acgctgcata taaacctttt aaatctttgt tcattatgaa gtccctccaa
    14521 aaatcatttg ataatatagt ttacagctat aattgtaaac gctatcataa aatgtaacaa
    14581 tatctttttg aaaattgtag tcatatttat gtataattaa tgaaattttt ttcaaaatca
    14641 atagaaatgg agtgagtaag gtgtattaca tcgcaatcga tattggaggc actcaaatta
    14701 aatcggcagt tattgataag caattgaata tgtttgacta tcaacaaata ccaacgccgg
    14761 acaacaaaag tgagcttatt actgacaaag tatatgagat tgtaacagga tatatgaagc
    14821 aatatcattt gatccaacct gtcataggta tttcatcagc aggcgttgtt gatgaacaaa
    14881 aaggcgaaat tgtatacgca gggccaacca ttccgaatta taaaggtact aattttaagc
    14941 gattattaaa atcactgtct ccttatgtca aagtaaaaaa tgatgtaaac gctgcattac
    15001 taggagaatt gaaattacat caatatcaag cagaacggat cttttgtatg acgcttggta
    15061 caggcattgg tggtgcgtac aaaaataatc aaggccatat tgataatggt gaacttcata
    15121 aggcaaatga agttgggtat ttattgtatc gtccaactga aaatacaacg tttgagcaac
    15181 gtgctgcaac gagtgcattg aaaaagcgca tgattgacgg aggatttacg agaagcacac
    15241 atgtgccagt attgtttgaa gcagctgaag aaggtgatga tattgcaaaa caaatattga
    15301 atgagtgggc agaagatgta gcagaaggga ttgcccaaat acaagtcatg tatgatccag
    15361 ggcttatatt aattggtggc ggtatatctg aacaaggaga caatctcatt aaatatatcg
    15421 agccgaaagt tgcacactat ttaccaaaag actatgttta tgcaccaata caaacgacta
    15481 agagtaaaaa tgatgcagca ttatatggct gtttgcaatg atggttgaaa gaaggagtca
    15541 ttctaaaata gaatttgaaa ccgttacgag agatgagagc tgttgttagt tccacagatt
    15601 atgttatatc catgaccaat ctaaactata tcaaccaaca gtgtgacacg ggcatattaa
    15661 attgaagaag ctgagatatt aaaattttag aaaatgtaaa aaatatttgg tattgaaatt
    15721 aaaaaagcac ctagcaactc gttgggacaa tcacgatgat tgtctacagt tgcaggtgga
    15781 tttgaatata ctactagtta tttgttgtct aggataatag atttagtatg ttgataagtt
    15841 tgactcagat tcgtattttc taataaatga tagctcacga tatcgattaa aaagagtgtc
    15901 gcaatttgtg tgttgataaa ttgatggtcg gtattacgcg attgatccgt tgttaaaagt
    15961 actaaatctg cacaatctgt aagtttacta ccttcgaaat ttgtgatggc aacgacatat
    16021 gcaccatgag atttggcgac ttccgctgca gaaattaatt ccgaagtatt accactattt
    16081 gacatagcaa taaacatatc cgaatgagat agtagggatg ccgatatttt cattaaatgt
    16141 gaatcggtag taacattacc ttttagcccc atacgaatca tacgataata aaattcagtc
    16201 gctgataaac cagagctacc tagtccagca aagagtatat gtcgacttga ttgaagtttg
    16261 tcgataaagg tttggataat gtcgttatca ataaattcac cagtttgttg aatgatttgt
    16321 tgatgatatt tatgaattct ttgaataatt gggctatttt caataactgt ctctgtcatt
    16381 tcttgttgaa tattaaattt taaatcttgg aaattctcat aatccagctt atgactaaag
    16441 cgtgtcatcg ttgctggtga tgtaccaatc gcatgggcta aggagttaat cgttgaaaag
    16501 gcatcgctat agccattttg tcttatataa ttgacgatgc gtttatcagt ttttgtaaat
    16561 aaatgttgat aacgttgaac acgattctca aatttcattg tgtcacccct tcatcttaat
    16621 gattactatt atatatgaaa aatattttca agatagtaaa aagcattgat aaaaattatc
    16681 ttaatgatat attgtaaatg actttacgtg aaaaaacgac ttatggagtg aggaataatg
    16741 ttaccacatg gattaatagt atcttgtcag gcactaccag atgaaccatt gcattcatct
    16801 tttattatgt cgaaaatggc attagctgcg tatgaaggtg gtgctgttgg tattcgtgca
    16861 aatactaagg aagacatttt agcaattaaa gaaacggtag atttaccagt tattggcatt
    16921 gtgaaacgtg actatgatca ctcagatgtt ttcattacag caacgtcaaa agaagttgat
    16981 gaactgatag aaagccaatg tgaagtcatt gcattggatg caacgttaca gcaacgtccg
    17041 aaagaaacgt tagacgaatt agtatcatat attagaacac atgcaccgaa cgttgaaatc
    17101 atggctgata tcgcgaccgt tgaagaagct aaaaatgcgg cacgacttgg ctttgattat
    17161 attggcacga cgttacatgg ctatactagt tatacgcaag gacaattact ttatcaaaat
    17221 gacttccaat ttctaaaaga tgtactacaa agtgttgatg caaaagttat tgcggaaggt
    17281 aatgtcatta caccggatat gtataaacgc gtgatggact taggcgttca ttgttcagtc
    17341 gttggtggtg cgataacacg accaaaagaa attacgaaac gttttgttca aattatggaa
    17401 gattaaatga taacgataaa aaacgagatg accatcatta attaaaggta cctaattatc
    17461 ttaggtggct gaatgaatgt aatgggttca tctcgttttg tttgtttatg atagtgattt
    17521 tattttcaac tttatccaaa aataagtaaa gcgacgggga tggtgattaa tagcgacaat
    17581 gccacgcgta aaaaccaaat gatgatgagt ttccagacag gtattttaat ttcagttgct
    17641 agtatacatg gcactaatgc tgagaaaaag ataatggctg atacgcttac tacaccgacg
    17701 acaaatttag tactcattgc agctttagtt actaacaaag atggtagaaa catttctaca
    17761 atagaaatcg ctgaagcttt tgcaagtaca gcctgatcag caattgggaa aatataaata
    17821 aatggataga atatgtagcc aagccaatca atgaatggtg tatagttcgc tacaatcagc
    17881 cctaaaaaac caatcgataa tatagaaggt aaaataccaa cagtcatttc taaaccgtct
    17941 ttcaaattgt cccaaacgtt cttcacgagg gatggtgtta atgcattttg tttcatcgcc
    18001 tctgcatatg cagttttcag tctgcttcct tcaatagcaa cttcttgttc tccttcttgt
    18061 ccgttataat attctgttga ttcattggtg attggcggta gccatgcagt aattgcagtc
    18121 acgacaaatg tgatgactaa agttatccaa aagtataaat tccaatgagg cattaatcct
    18181 aaagttttag caatgataat cataaaagtt gctgaaactg ttgaaaagcc agtcgcaata
    18241 atcgtggctt ctcgtttgtt gtacatccct tgcttataga cacgattagt aatcaataat
    18301 cctaaggaat aactgccgac aaacgaagcc actgcatcga tagcggattt tcctggtgtt
    18361 ttaaaaatag gtctcataat aggctccata taaacaccga caaattctaa taagccataa
    18421 cctactaata aagaaagcgc aattgcacct actggaatta agatacttaa tggcatcatt
    18481 aatttttcaa acaaaaacgg accatagtta gctttaaata gtattgatgg accgaattta
    18541 aatacataca ttataccgat cattgcacct gcaactttaa ataatgtaat gaccaagttt
    18601 gtgattgaag tcataaaagt acgtctcact attggtaacg ctgtaccaat taaaatcata
    18661 atcagtgcaa catagggcat aagtggacct atgattgagc gaatggctag atgaacatga
    18721 tcgacgaaaa tagtgttgtt accattaatc gtaaaaggaa taaagaaaca tagtatgccc
    18781 actaaactat agacaaaaaa acgccatgca cttggttgtt gtgcattaga atgatattga
    18841 ttcattaaag caaccccttt gtttaaatga atacacaaaa ctgtatgatg catcttcccc
    18901 ttaatgagat gaatcattgt tttaatttag aaaaatctga aaacttacta taattgtata
    18961 gtttggatta ttttcagacc aatacaaa
//
LOCUS       DAKSQE010000049        18710 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.049, whole genome shotgun sequence.
ACCESSION   DAKSQE010000049 DAKSQE010000000
VERSION     DAKSQE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18710
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.049"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(1..407)
                     /locus_tag="O0V33_001415"
                     /pseudo
     CDS             complement(1..407)
                     /locus_tag="O0V33_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019835092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            506..1471
                     /locus_tag="O0V33_001416"
                     /pseudo
     CDS             506..1471
                     /locus_tag="O0V33_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
     gene            1482..2098
                     /locus_tag="O0V33_001417"
                     /pseudo
     CDS             1482..2098
                     /locus_tag="O0V33_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
     gene            complement(2329..2940)
                     /locus_tag="O0V33_001418"
     CDS             complement(2329..2940)
                     /locus_tag="O0V33_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCX8976052.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
     gene            3491..4768
                     /locus_tag="O0V33_001419"
     CDS             3491..4768
                     /locus_tag="O0V33_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCX8976053.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
     gene            5245..7068
                     /locus_tag="O0V33_001420"
     CDS             5245..7068
                     /locus_tag="O0V33_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCX8976054.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMILGTLTATTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGVLATMMVLITILLSKNKAEEQAIQNDFKLISKLLHAYN
                     DKSAFSQVAKTAVDSFKASDKLLITSNSSSDKLSGRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIITQRDDRNKNVRYWRKEVTVTEEFQNLFNHILKIDEMVHA
                     NEARIAYEAEMRKPLYSKRIYQNLTLDSIVFRNTLRYAAIMMIAIFIALMFDFEKAYW
                     IPLSAHTVLLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYTIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLG
                     QRTASALLPYVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLATFENIAK
                     HLELNTELEIKILPDLPQYNYIQSAMMNIQHNGFRERDKNV"
     gene            7207..7623
                     /locus_tag="O0V33_001421"
     CDS             7207..7623
                     /locus_tag="O0V33_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCX8976055.1"
                     /translation="MPFTMKGYPEKWKEFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKPEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGGVRQREPLMDGPHPKVVESFKDV"
     gene            7854..8546
                     /locus_tag="O0V33_001422"
     CDS             7854..8546
                     /locus_tag="O0V33_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCX8976056.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNKAETEKYRDLLKECATEITEVE
                     NKLQQIHSEITE"
     gene            8711..9442
                     /locus_tag="O0V33_001423"
     CDS             8711..9442
                     /locus_tag="O0V33_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCX8976057.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLD
                     KIKNVQYYVNEASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSILSS
                     ILMKNKWPFLIALPFMIGYGIGITIYYQRKVAYLCPNCQHIFSPSLWAVIKAKHTATT
                     RRFECPNCHETHYCIEVPKTHMGTE"
     gene            9629..10309
                     /locus_tag="O0V33_001424"
     CDS             9629..10309
                     /locus_tag="O0V33_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCX8976058.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            10334..11887
                     /gene="gntK"
                     /locus_tag="O0V33_001425"
     CDS             10334..11887
                     /gene="gntK"
                     /locus_tag="O0V33_001425"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCX8976059.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYIMRESKVNQDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLAKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDRYIIDYSMASATGMFNLETLDWDVGALELLGISEEMLPELVPTTYVMKGM
                     KERYATLMGLNEDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHTPIEENVAVYQELVSIFINLSRSLTENYEQIADFQR
                     KHMAENKTQ"
     gene            12004..13362
                     /locus_tag="O0V33_001426"
     CDS             12004..13362
                     /locus_tag="O0V33_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HCX8976060.1"
                     /translation="MFNEIWPLISVVLGIIILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            13744..13846
                     /locus_tag="O0V33_001427"
                     /pseudo
     CDS             13744..13846
                     /locus_tag="O0V33_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13830..16727
                     /gene="fnbA"
                     /locus_tag="O0V33_001428"
                     /pseudo
     CDS             13830..16727
                     /gene="fnbA"
                     /locus_tag="O0V33_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbA"
     gene            16907..17773
                     /gene="galU"
                     /locus_tag="O0V33_001429"
     CDS             16907..17773
                     /gene="galU"
                     /locus_tag="O0V33_001429"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCX8976061.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIIEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKELGL"
     gene            complement(17900..18208)
                     /locus_tag="O0V33_001430"
     CDS             complement(17900..18208)
                     /locus_tag="O0V33_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976062.1"
                     /translation="MTVNIVFSIVFCISMVILGIYVAITKDFTLISFINQTTIADKHK
                     TQIAYIFALCISLSAVFLMSAILCFEYDFIALSFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(18352..18618)
                     /locus_tag="O0V33_001431"
     CDS             complement(18352..18618)
                     /locus_tag="O0V33_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976063.1"
                     /translation="MKGSKQILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAH
                     TLNSSSAIIEQHTMSNLASLDLYAPVRNITSSRDIHPIYFMTKD"
BASE COUNT         6708 a         2703 c         3541 g         5758 t
ORIGIN      
        1 atcattagtt aatatttttt taaatatatg gtttggaatt agttttggtg atagttctcc
       61 agtacctaat cggacgatat cagtattaaa ttctatttca ttaatttttt gaatataata
      121 ctggttattt ttagaaaagc tgttgcctat ataatcttgc caatgaatat tattggtaat
      181 ataagaattc catttatact gggatacata aatacctttt tttcaattgt atctaagatg
      241 ccatagcttt ttagaatatc taaagcttgt ataactgtgg atttatttac attatagtac
      301 ttagctaatt ttctttgcga gtacaattta tctccaggag ataaaatacc gttgttaatt
      361 ttattaataa tatcatcaat aattttttta tattgaaatt tagtcataaa tgtcatcctc
      421 aatctggttg gaatttttat tagaatctgg taggtttttt aattttcaga ttattataac
      481 atattaaata agggaggcgg ttaaattgac actaaaatta gaaaaagctg tattttcaat
      541 ggagataaaa aaagatttga agttattaat gaaaaaggat aattaccaca atattttcgc
      601 tttgattttt gactggttgg taattctcgg gagtgcatgt ataagtatct atatgagtaa
      661 tctcattatt tatattataa gtattatttt aattggaagt cgaatgaggg cgtttgataa
      721 cttaatgcac gaagcatgtc atcgttcatt atttacaaat aagttttata ataaatggat
      781 tgcttgtctt ttagtagcat tccctatatt tacaagcttt actacatatt gtaattctca
      841 ttttaaacat catagaaatt tatgggatga ggaaaaagac ccagatacta aacgatatcg
      901 cattgtaggt ttagataagc cgcaaaaaga tgtgaaaaaa tttataatta atcacattgt
      961 aaaggtacta tttttattcc atgtgcctaa atatgtttta ggtacagttt cagctaactt
     1021 atacagtagg gatacaccta aatcagaaat atggactaga aatattttct gggtaacaat
     1081 tattacgtta tcaattatgt ttaatttttg gttatatgtt attctttatt ggtttatacc
     1141 acttttaaca acttttcaga ttattcgtta ttgggcagaa atggctgaac actctggatt
     1201 gaataatgat agtgagttaa cagcaagtcg gaatactttt ggtatgcctt ggacaatatt
     1261 tttatttcat cctcaccatg ataattttca cttagttcac catttattcc cagcaattcc
     1321 acattataat ttgaaaaaag cacatattat attaatgaaa gatcaaaaat atagagaagc
     1381 acaccattgt actgggttct tcaagacgac actaccgggt ttttatagtg tagtaaaaga
     1441 tatttgtaca ccgaacattg ttgggaaggg gaggatgcga attgatacaa ccagttttac
     1501 acggtatttt attagcattg gggcttattt tgcccctagg agcgcaaaac gtgtttgtat
     1561 ttaatcaagg tgctaatcaa aaaaaatgat aaacgcattg ccggttgtta taacggctgg
     1621 attatgtgac acttttttaa taataattgc tgtactgggt gtttcactaa tattaatgtc
     1681 attaccagtt ctacaattat ttatttatat agttggtttg ttttttctat tgtacatggc
     1741 ttggtcttta tggaaagaaa agcctaacac tctagagaac tatgaaccga tgagtacaaa
     1801 aaagcaaata ggttttgcta tatctgtttc gttgttaaat ccacatgcaa ttatggatac
     1861 agttggtgta attggatcaa gcgcatctgt ctattctggt ttagaaaaga tacttttttg
     1921 tattgccaaa attatggttt cttggctatg gttttttctg ttagcaatat taggtaaggt
     1981 tttaggttct atagatagaa caggtaaata tatattattt ttaaataaaa tctctagtat
     2041 aataataatt gctgttagta ttgttattgt aaaaaatata ttaaagttaa ttttataata
     2101 taaaagtgca tattagttat aattggaaaa acagtaagtg atatttaaaa atgaaaagat
     2161 tttaatatta taattaatct tgaggtgttg agctccttag aattagttga caaaaaagat
     2221 tggaaatctt aactgaaaaa atgtaacatt attatgaaat tatataaaaa tataaagaag
     2281 aacctcggag gaataaattc gaggttcttc tttatgctaa caacaatatt attttactct
     2341 cttacgtttg ccaatgtaac gtatagcaaa gtaaatgact gcgataatga tgacaacata
     2401 cataatacgt gaatatgtat gaagaccagt catcaacata ccaaagctat cgctcaacgt
     2461 gcgtcctagt aaaattaaac cgaagttcca aattgttgta cctattaatg aaataacggt
     2521 aaatgtcaca acattcatgc ggtttacacc ggctggaatg gtaatcaata cacgtaatac
     2581 aggtataaaa cgacagataa atacagccca tacgccatac tttttaaacc aatcatttgc
     2641 tcgctttaaa tccttacttt tcaatttaat ccacttaccg tgtcgatcaa taaagcgata
     2701 tagacgttct tctgaaatca aacgacagat ataatataaa attaacagtc ctataaacga
     2761 tgcgatggtt gcaataataa ataaggttaa aattgataaa tgtgatttaa cagacataag
     2821 accagcaaat gttagaataa tttccgatgg tacgataggt aaaacatttt ctaataaaat
     2881 taatataaaa atggctgcat acccaaaacg actaataaat tcagtgataa tttgttccat
     2941 tattaaaaat aagctccttt aattttcagc aattgcagac aaatagaaac tggataaatt
     3001 caaatgaata atgaatgatg tcgtatgcga tgttgaaaca agcatttgtc ttaaaatgta
     3061 ttataagcaa taaatcaaaa aattgcttgc tataagtacg aaaagtcaac aaatgatcat
     3121 ctaaataaca ggcgctgcac aatgaatgag taatgtagtt ttatgcaatt taaaatgaat
     3181 gtcaaatatg taagaaatcc tgcaatatac gttttaaaac actagtattg cagtattttt
     3241 aattaacaaa tgataggcta ctatgtaact aaaagttcct ttatatcgtt ttaatagcaa
     3301 aagttaagcg gaatttaata aaatttttat agattctatt gtaaacgctt tccatgtctg
     3361 ataatattat aaaaaataac tttggtacaa ttttgtgaca aagtaggggt ggacatgcca
     3421 gatgtccgaa aattttaacg tttcagggta agttttaaaa aattaattaa ttagggaagt
     3481 aggaatagtt atgaaggaaa aacgaactaa cgtaaggtgg atgttcgcgc ttgcgttctt
     3541 ctttattggg gttattgcgt atatggatag agcaaacatt tcatatatcg ctaagcaaat
     3601 gatggatgat ttagggatga caaaaccaca atttggttta ttggcatcat tcttctctct
     3661 aggttatgca ttaatgcaag taccatcggg gatgttggct gaaaaattcg gtccacgtaa
     3721 gatgattaca attgcattag tttggtggag tgcatttaca atcttaacgg gtatgattaa
     3781 gaaccacggt ttaatttatt tagtgagatt cttatttggt gtaggtgagg cgccaatgta
     3841 cccttctaat gctgtgttta actcattttg gttctctaaa aatgaaaaag gtagagcatc
     3901 aagtgcatta ttagcaggat catatttcgg acctgtatta gcaccaatag ttacaattgc
     3961 tattgttaac gcatttaact ggcaagcagt attttatatt tttggtgcag tagggatttt
     4021 aatggctgta ttatgggcga ttattgctaa agacttacct gagcaacata gaatggttaa
     4081 tgaagcggag aaacgtttca ttatggaaaa ccgtgatatc gtagctactg aaaagtcatc
     4141 accaccatgg aatgatttct ttaaacgttt tagcttctat gcaattgcaa ttcaatactt
     4201 tgttgtacaa tttatcatta cattattctt aatttggtta ccgacgtatt taacagaagt
     4261 attccacgtt aactttaaag aaatgagcat tagttcatta ccttggttat taatgttctt
     4321 cttaatctta tcagcgggtg caatttctga ccgtgtatta ggattaggtc gttcaaaatt
     4381 cgtagctaga ggtgtaattg caatcgcagg atttattgta tttgcagttt caattatctt
     4441 tgctgtacgt acaggaaatt tatatgtaag tattttctgg ttatcactag gtcttggtgg
     4501 tatcggtatt tcaatgggta tgagttgggc agcagcaact gatttaggac gtaacttctc
     4561 tggtacagta tcaggatgga tgaacttatg gggtaatata ggtgcattaa tcagtccgct
     4621 attagcaggt ctattcgtag aacatttagg ttggacaatg acattccaat tgttaatcgt
     4681 tccagcagta atcgctgtga ttatgtggtt ctatgtgaaa ccagatcaac ctttaattgt
     4741 tagtgatgat aaagcaatag aaaaataatt taatcatgca ataagctttc acatagttgg
     4801 ggcttattgc ttttttcgtt gaaattgaaa aattttaaaa cagatatggt ttaagatgaa
     4861 cgggaagtta ttaaaataat atatgtagaa gaaataaagt tttaaaacct tagtcaggta
     4921 actattgtaa aagtacaaat aaattttaac tagcgcaaag gtgggcgacc aaaggttcaa
     4981 cgatgttaaa taacattaga aaataatttt aattggactt taaaagtttt taaatttaga
     5041 taattgagca taaggtgtta taatgacata tgttgcgtaa ttgaaattta tagcaacata
     5101 ctcaaattaa ctatgctaat aaaatgatta tggaaatatt ttgacgagga agggagaagt
     5161 cgaaatgaca tctttttgac atcacttata aaaattaatc gacttaactt agacttttat
     5221 aaagggataa gacaaggact gttaatgatt attcctgcaa tcatcggtta cttatgtggt
     5281 aatttccaat ttggattatt agttgcaacc ggaactctag cacatatcta cgtttttaaa
     5341 ggtccgtcgc gatctaagct gcgaactgta ataatttgta atttagcgtt tgcaatatgc
     5401 atgatacttg gtacgctaac agccacaacg ccgctcgttt ttggaatgac attattaatt
     5461 gttacggtta taccatttta tatatttacc gccttgaaaa ttgctggacc gtcatcgaca
     5521 ttcttcattg tgacattcag tttacccatt aacttaccta tagcacctga agaagcatta
     5581 tatagaggct ttgcgatttt agtaggtggt gtacttgcca ctatgatggt actcatcaca
     5641 atcttattgt ctaaaaacaa agctgaagaa caagccattc aaaacgattt taaactcata
     5701 tctaagttat tgcatgctta caatgataaa tctgcatttt cacaagtagc aaaaacagcg
     5761 gtggatagtt ttaaagcgtc ggataaacta ctcatcactt ctaattcaag tagtgataaa
     5821 ttaagcggac gtttccaaaa attattgtta ttacacacat cagcacaagg gatatattct
     5881 gaattattag agctgaatgc taaacaaatc agaccattgc cagatgagtt aattgaaatg
     5941 atggatcata tcattacaca gcgagatgat cgcaataaaa atgtgagata ttggcgcaaa
     6001 gaagtaacag taacagagga atttcaaaac ttattcaacc atatcttgaa aattgacgaa
     6061 atggtgcatg caaatgaagc acgtatcgca tatgaagctg aaatgcgaaa acctttatat
     6121 agtaagcgta tttatcaaaa tttaacatta gattcaatcg tgtttagaaa tacattgagg
     6181 tatgcagcga ttatgatgat agcgatattt atcgcgttaa tgttcgattt tgaaaaagca
     6241 tattggatac ctttatccgc acatacagta ttattaggaa catcaactat acatgcaatt
     6301 gagagaggta tggcacgtgg attaggcacc attttaggtg tgctagtact ttcagttata
     6361 ttgttgtttt caataccaac acctgttgca gtcattttaa tgggcattgc agcattattt
     6421 actgaagcgt tggtgggagc aaattataca attgcagtag tttttattac aatacaagtt
     6481 attttaatga acggattagc atcacagaac ttaacaatta acattgcgtt tccaagagtt
     6541 attgacgttg caatgggtat tgtgattgca atcataggtt tatttgtcct tgggcaacgt
     6601 accgcatccg cattgcttcc ttatgtaatg gctgaagttg ttcgtaaaga agcaacgctc
     6661 ttccattatt tattttctga aaaccaatat aaagatgatg tgtatcagaa aaacacggct
     6721 atgaacctat ctgtgaaatt aaataatatg acgcaagttt ataatgcagc taatggtgaa
     6781 ttatttagtg ataaaacatt gattcaaaat tattatccta gcttattcgc attagaagaa
     6841 attagcttta tgttaaatag agccatggct aatgaagata gacttacaat aaatgaacaa
     6901 ttaatgggcg agtatttagc aacatttgaa aacatcgcta agcatttaga attaaataca
     6961 gaattagaaa taaagatatt accagattta ccacagtata attatattca atcagcaatg
     7021 atgaatattc aacacaatgg ttttcgtgag agagacaaga acgtttaaaa taaattattt
     7081 atcaggtcag actaacgttt gtctgaagtt attatgacgg gagtctaaaa aattgtgttt
     7141 aatatgtttc attttataat tatggtggca tttaacatga attataacat attggaggtt
     7201 aaggaaatgc cctttacaat gaaaggttac cccgaaaaat ggaaagaatt tgaagtaatt
     7261 aaacggcaaa aggcgataga aattgcgaat gcaatgttaa aagaaggata tcttgaaaaa
     7321 gatgttgttc ctattgcggc gaaaaaagcg aaagattggt atcgagcgtt atcgaaagaa
     7381 gaaatcaaag cattagaaga agagaacttc gtacaattcc atacagcaac aatggaaaaa
     7441 ccagaggtgg atagtgaata tgcaacaact gataatgaag aacatattca agaacaatca
     7501 aatcaagata aatcgtactt tgatagcgaa tcaattggca accaaagtgg tggcgttcga
     7561 caaagagaac cgcttatgga tggtccgcat ccaaaagttg tggaatcatt taaagacgta
     7621 taacatctct atcaattaag cacttgtacc tatataacgg taacaggtgt ttttttgcgt
     7681 taaatcattt taaaattact tcctataatt gaaggttttg atacataatt cgaattgttt
     7741 gagtttcttt gtgctgtttc agtatcagcc caaagttcga tagtgtatta gtattatctt
     7801 aataaaatgt taggtacaat aaagatgatt atatatcgga ggtaagtata aaaatgtatg
     7861 tagatcgaaa accatcacta tatttagagg atttgcgaca tgattttaaa aatagtttaa
     7921 gtaaatttga aaatggtgat gaagcatttg atacgttatt aggtttcgta gagttagatc
     7981 atatttattc atcagcacta aaggaaataa gcacaaaact gagtatttta gatgacaatt
     8041 tcaatcacat ttataaacac aatcctatac atcatatgga gcgacgtgtg aaagaaatgc
     8101 gtagtttaat agaaaagctt aatcgtaaag gattacagat tagcgcagaa actgccaaag
     8161 aacatatact ggatattgcg ggaattcgcg tagtatgtaa ttacttagat gatatttatt
     8221 tgattgaaga gatgttgctt aaacaagaag acgtacaatt gataaaacgt aaagattata
     8281 ttcagcaccc taaagaaaat ggttaccgca gtttacatat cgttgtatcc attccagtct
     8341 ttttagcaga acgcgttgag gtattgcctg ttgaaattca aattagaaca ataggtatgg
     8401 atatgtgggc aagtttagaa cataaaatac gttataaaaa caaggcagag acggaaaagt
     8461 atcgagattt attgaaagaa tgtgcgacag agattactga agttgaaaat aaattacaac
     8521 aaattcattc tgaaataaca gagtagacgt ttagtaggtg tgttgcttaa tttgagtgat
     8581 tttgtaaatc aacatggtat gaaatcacaa aaataagacg cctagtataa taaatttctt
     8641 aaaaatgtgg aaattatact tgctcgtgac gtcgggtcat caactatggt gattgtagga
     8701 ggtggaatct atgtctaact attcgactgg agaactcgca aaattatgca atgtgacaac
     8761 acgaacgatt caatattatg atcgcaaagg tattttgaaa ccacaaggat ttacagaagg
     8821 aaagcgtcgt gtgtatacag aacaacagcg acaaacatta gagttaatct tattacttaa
     8881 agatttaggt tgtgcgttaa gcgatataga tatgttgcta aaaggtgaag gtactttgaa
     8941 gacactcaat accttactaa ctatgaaaca acaagaaatt aatcaacaag tcaaacagca
     9001 acaagcggta ttagacaaaa ttaaaaatgt tcaatattac gtaaatgaag cgtcgacgtc
     9061 tccaatcaca catttaaaag acatagagca tgtcatgagt aaatcagctg aaatgaaaag
     9121 tattcgtcgt aacatttgga ttagtgctgg tattatagga ataattcaat attctagcat
     9181 tttgagttca atcttgatga aaaacaaatg gccgttctta attgctttac catttatgat
     9241 cggttatggc attggtatta ctatctacta tcaacgcaaa gttgcctatt tatgccctaa
     9301 ctgccagcat atattctcac cgtctttgtg ggcagttatc aaagcgaaac atactgcgac
     9361 aacacgtcga ttcgaatgtc caaactgtca tgaaacgcat tattgcattg aagtacctaa
     9421 aacacatatg ggtacagaat aattagacac atcccacata caacataaca attaaatcat
     9481 tcgtgtgata gtgaccattc cctttaaatt tacattgaag aggggtggtc actattttat
     9541 tacgcgtaaa gaaagcgctt gcaaacactt aagaactata ttaacatact tgtatacaag
     9601 tatgtcgtgt aatgaaggag tggctttgat gatgtatgga tatccagaga aatggttgga
     9661 aggtatgaca actggagaag gtatcgcagc agaattacgc ttaggcattg tgaatggtca
     9721 catagctgaa ggtacgttac tcactgaaaa tcaaatggca aagcaattta atgtgagtcg
     9781 ttcgccaatt cgagatgcat ttaaattatt gcaacaaaat caactcatcc aattagaaag
     9841 aatgggtgca catgtgttgc cgtttgggga acaagaaaag aaagaaatgt acgatttgcg
     9901 actgatgtta gagtcatttg cattttcaag agttaaaaat caagagcgac tacctatcgt
     9961 aaaagaaatg aagaaacaac ttgaaatgat gaaagtggca gtaaaatttg aggatgcaga
    10021 atcatttacg aagcatgact ttgaatttca tgaaacatta atcaaagcat ctaatcatca
    10081 atatttaaac tcattttgga gtcatttaaa accagtaatg atggcgctcg ttttaacatc
    10141 catgcgacaa cgtatgcaac aaaacccgca agattttgaa cgcatacatc ataatcatca
    10201 agtgtttatt gatgcagtgg aacaatacga cagtcaaatt ttgaaagaag cgtttcattt
    10261 aaatttcgac gatgttggta aagatattga aggattttgg ttgaattaaa atgaaaagaa
    10321 agaaggattt gtcatgaaat atatgattgg tgtcgacatt ggaacgacga gtaccaagtc
    10381 agtcttatat gatgaaaatg gaactttcat catgaaacat caaatcggct atgatttaca
    10441 cacaccaaac gttgatgtct cagaagaaaa cccagatgaa ttatttgatg cggtattaat
    10501 gacaattaaa tacataatga gggaatcgaa agttaatcaa gatgatatta aatttgtgtc
    10561 atttagtgcg caaatgcata gcttgattgc gatggatcag caacatcaaa gattaacaaa
    10621 taatattact tgggcagata accgcgctgc aaaatatgca acagtaataa atgaagtgca
    10681 tgatggcaat gcgatttatc agcgaacagg tacgcctatt catcctatgt cgccattagc
    10741 gaagattttt tggatgaaac atgaatggca agatgtattt caacgaactg ctaagtttgc
    10801 agatattaaa acatacattt tctatcattt atttgataga tatatcattg attattcaat
    10861 ggcttccgca acagggatgt ttaatttaga aacattagat tgggatgttg gggcattaga
    10921 attgcttggt atttccgagg aaatgttgcc ggaattagtg ccaacaacgt atgtgatgaa
    10981 aggtatgaaa gaacgttatg caacattaat ggggcttaat gaagatacac cgtttgttat
    11041 tggtgcgagt gatggggtcc tttctaattt gggtgtcaat agtgttggta aaggagaagt
    11101 tgctgtcaca atcggtacat ctggggcgat tcgtactgtg atagataaac cacgtactga
    11161 ttacaaaggt agaatatttt gttatgtctt aacagaggac cactacgtca ttggaggccc
    11221 tgtaaataat ggtggtgtcg tattgagatg gttgcgcgat gagttgttag caagcgaagt
    11281 cgaaactgcg aaacgtctcg gtgttgatcc ttatgatgtt ttaactcaaa ttgcaaagcg
    11341 tgttaaacca ggtgcagatg gtttaatatt ccatccctat ttagctggag aacgtgcacc
    11401 gctttggaac gcaaatgcaa gaggttcatt cttcggttta actttatctc ataaaaaaga
    11461 acatatgatt cgcgctgcat tagaaggtgt tctttacaat ttgtataccg tctatcttgc
    11521 gcttattgaa gtaatgaatg aaacacctaa gatgataaaa gcaacaggtg gttttgcgaa
    11581 aagtgaagta tggcgtcaaa tgatgtcaga tatatttgac acagagttag tggttcctga
    11641 aagttatgaa agttcatgct taggtgcctg cgtactcggg cttaaagctg taggtgacat
    11701 tgaagatttt tcaatcgttt catcgatggt cggtgccaca aacaatcata cgccgattga
    11761 agaaaatgtc gctgtttatc aagagctcgt atccattttt atcaatttaa gtcgttcttt
    11821 aacagagaat tatgaacaaa ttgcagattt tcaacgcaaa catatggctg aaaataaaac
    11881 acaataaata cgtcactcaa gcatcttaga taaagttgtt gtgccatgac tactatatat
    11941 tatgtggaat acgtgcgcga cgacggtgtt acattctatt gaaaagagaa agaggggaat
    12001 gtcatgttta acgaaatatg gccgttaatc agtgttgttt taggtataat tattttatta
    12061 gtattaatca ttgggtttaa attaaataca tttatttcat taattatcac atcgatgatt
    12121 acagcgttaa tgctaggtat accattgact aaaattatgg agacgattga gaaagggatg
    12181 ggcagtacgc tcggccacat tgcactgata ttcggtttag gtgccatact tgggaaatta
    12241 cttgctgatg gtggtggtgc aactcgaatt gcagacacgt tgattcaaaa atttggtcaa
    12301 aaacatgtac aatgggcaat gcttgttgca gcatttatcg tcgggattgc attgttcttt
    12361 gaagtaggtt tagtcttact gattccatta gtatttacag tagcaaaacg cgcaaatgtt
    12421 tcagtgttaa aactaggatt acctatggta acagctttat cagtgacaca tggcttttta
    12481 ccaccacatc caggaccggt agtcatcgca aaagaattaa aggcgaatgt aggagatgta
    12541 ttactatacg gtatgattat tgccattcca gttacactca ttgcaggacc tatatttaac
    12601 aaagttgcac aaaaaatgat tccgtctgcg tatacacgag aaggcgatat ttcagcgtta
    12661 ggtgcacaaa aagaatttac ggatcaagag atgccaggat ttggtatgag tttattaaca
    12721 gcaacattac cagtcatatt aatgttagtg tctacgataa cgcaacttgt aaccgggcac
    12781 gacaaaccta caaatctatt tgaatctatc atttatatga tcggaacagc agggacagcc
    12841 atgctaatag cagtattatt tgcaatcgtt acgatgggat taatgagaaa acgtaagatg
    12901 aatcatatta tggaatcagt gacgaatgcg atttatccaa tcggaatgat gttattgatt
    12961 attggcggtg gcggtacatt taaacaagta ttaattgacg gtggtgtcgg aaatacaatt
    13021 gctaaaatgt ttgaaggtac agagatgtct ccgattttac tagcttggat tgttgcagct
    13081 gtgctacgta tcgcattagg ttcggctaca gtagctgcga tttcaactac aggtattgtc
    13141 ttaccattat tacaatcatc agatgtaaat gttgcattag ttgtactagc gattggtgca
    13201 ggtagtgtga ttttgtctca tgttaatgac gcaggattct ggatgtttaa agaatatttt
    13261 ggtttaactg taaaagaaac attcctaaca tggtcattat tagaaacgat tatttccgtt
    13321 tcaggtatca tcttcatttt attcatcagc ttatttgttt aactaaatat taagtaaacg
    13381 tgtttttagg taattacaat atattgcctc gaaacacgtt tatttttatg cagaaaatcg
    13441 tctgaaatac tcagaatccg aacgatataa aaatatattg gttgttttag tgtaatgaaa
    13501 ggcgtttcaa gacatgtttt tgtgcgtatt gtacaggcga taattatgaa acgtttagta
    13561 tgttgtttta aattagataa tgataaattt aatttaaaaa ataagtataa aaaatacaag
    13621 ccttgtgtga caagggtttc tgacaacttg gatacaattt ataagtatat ttcaaataat
    13681 aaaattatca attaacataa aattaatgac aatcttaact tttcattaac tcgctttttt
    13741 gtattgcttt taaaaaccga acaatataga cttgcattta ttaagtttaa aaaaattaat
    13801 gaattttgca tttaaaggga gatattatag tgaaaaacaa tcttaggtac ggcattagaa
    13861 aacataaatt gggagcagca tcagtattct taggaacaat gatcgttgtt gggatgggac
    13921 aagataaaga agctgcagca tcagaacaaa agacaactac agtagaagaa aatgggaatt
    13981 cagctactga taataaagta agcgaaacac aaacaactac aactaacgtt aatactatag
    14041 atgaaacaca atcatacagc gcaacagcaa cagaacaacc gtcaaacgca acacaagtaa
    14101 caactgaaga agcaccaaaa gcagtacaag cgccacaaac tgcacaacca gcaaatgtag
    14161 aaacagttaa agaagaggta gttaaggaag aagcgaaccc tcaagttaag gaaacgacac
    14221 aatctcaaga caatagcgga gatcaaagac aagtagattt aacacctaaa aaggctacac
    14281 aaaatcaagt agcagaaaca caagttgaag tggcacagcc aagaacggca ttagaaagta
    14341 agccacgtgt gacaagatca acagatgtag cggaagctaa ggaagctagt gacgcgaaag
    14401 tggaaacggg tacagatgtg acaagtaaag ttacagtgga agatgaaagt aaaattgaag
    14461 cgccaaaagg aaataatgtt cagcctcatg aagggcaacg tgtagtatta aagtataaac
    14521 tgaaatttca agatgggttg aaaacaggcg attattttga ttttacatta tcaaataatg
    14581 taaatacaca tggtgtagca actacaagaa aagttccaga tattaaaaat ggatcattag
    14641 ttatggctaa aggtcaggta ttagataatg gccgaattag atatacattt actgattaca
    14701 tcaaagataa agtaaatgtt acagctaatt tagaaataaa cttattcata gatcctaaaa
    14761 ctgtacaaag caatggacaa taaacaataa cttcaaaatt aaatggcaaa gaaacatcag
    14821 gaactatgca aataacatat aaagatggtg ttaaaaatca atatacaaat gtaaatggtt
    14881 cgattgaaac gtttgacaaa gaaaaaaata aatttacaca cgtagcatat attaagccta
    14941 taaatggaaa caattcagat agtgttactg taacgggtat gttgacacaa ggaagtaatg
    15001 agaatggaac acaaccaaat gttaaaatat atgagtatgt tggagttgaa aatgggctac
    15061 cacaaagtgt ttatgcaaat acagtggaca gcacacaatt gaaagatgta acgaatcaaa
    15121 tgggtgataa attgaaagta caaaacaatg gtagctactc attaaatttt gacaaattag
    15181 ataaaacata tgtaattcat tacaccggag attacttaaa tggtacgagt gaagtgaact
    15241 ttagaactca attaactgga taccctgaaa atcgctataa aacatattat tattataaca
    15301 atggctatac attaacatgg gataatggat tagttttata tagtaataaa gctaatggtg
    15361 atggaaaata tggtccaatc gtagactcca ataactttga gttctctgaa gatagtggta
    15421 atggttcaat cagcggacaa tacgatgcga agcaaattat tgaaacagaa gaaaatcaag
    15481 acaatacacc gcttgacatt gattaccaca cagctataga tggtgaaggt ggttatgttg
    15541 atggatacat tgaaacaata gaagaaacgg attcatcagc tattgatatc gattaccata
    15601 ctgctgtgga tagcgaagcg ggtcacgttg gaggatacac tgagtcctct gaggaatcaa
    15661 atccaattga ctttgaagaa tctacacatg aaaattcaaa acatcacgct gatgttgttg
    15721 aatatgaaga agatacaaac ccaggtggcg gtcaggttac tactgaatct aacttagttg
    15781 aatttgacga agagtctaca aaaggtattg taactggcgc agtgagcgac catacaacag
    15841 ttgaagatac gaaagaatat acaactgaaa gtaatctgat tgaactagta gatgaactac
    15901 ctgaagaaca tggtcaagca caagggccag tcgaggaaat cactgaaaac aatcatcata
    15961 tttctcattc tggtttagga actgaaaatg gtcacggtaa ttatggcgtg attgaagaaa
    16021 tcgaagaaaa tagccatgtt gatattaaga gtgaattagg ttacgaaggt ggccaaaata
    16081 gcggtaatca gtcattcgag gaagacacag aagaagacaa acctaagtat gaacaaggtg
    16141 gtaacatcat tgatatcgac ttcgacagtg tgccacaaat tcatggattc aataagcata
    16201 atgaaattat tgaagaagat acaaacaaag ataaacctaa ttatcaattc ggtggacaca
    16261 acagtgttga ttttgaagaa gatacacttc caaaagtaag cggtcaaaac gaaggtcaac
    16321 aaacgattga agaagataca acgccgccaa caccaccgac accagaggta ccaagtgagc
    16381 caggcacacc aacaccgcca acgccagaag tgccaagtga gccaggtaag ccaacaccac
    16441 caacaccaga ggtaccagct gaacctggta aaccagtacc acctgctaaa gaagaaccta
    16501 aaaagccttc taaaccagtg gaacaaggta aagtagtaac acctgttatt gaaattaatg
    16561 aaaaggttaa agcagtggca ccaactaaac aaaaacaagc taagaaatct gaactacctg
    16621 aaacaggtgg agaagaatca acaaacaaag gtatgttgtt cggcggatta ttcagcattc
    16681 taggtttagc gttattacgc agaaataaaa agaatcacaa agcataatca atctaaaatt
    16741 gacaggttta tttcataaat tatatgaagt aagcctgttt tttaaaatta aatcaaattt
    16801 cccaagaaat aattacatac tcaatgacac tatgaaggcg ttctaattag tgttaaaatg
    16861 acgttgatac atagatttaa tacttaggaa aaggagcaca ttaactttga aaaaaataaa
    16921 aaaggcaatc attcccgctg ctggtttagg gactagattt ttaccagcaa ctaaagcgat
    16981 gccaaaggaa atgcttccta tcttagataa accgacaata caatatatca ttgaagaagc
    17041 tgcaagagct ggaattgaag atattattat agtgacaggt cgccacaaac gcgcgattga
    17101 agatcatttt gatagtcaaa aagaattaga aatggtgtta aaagaaaaag gtaaatctga
    17161 attactagaa aaagttcagt attcaacgga acttgcgaat attttttatg taaggcagaa
    17221 agaacaaaaa ggtttagggc atgcgattag ttctgcgcgt caatttatcg gcaatgaacc
    17281 atttgcggta ttattaggtg acgatattgt tgaatctgaa gtgccagcag tgaaacagtt
    17341 gattgacgtt tatgaagaaa cggggcactc agttataggt gttcaagaag ttccggaagc
    17401 agatactcat cgttatggaa ttattgatcc attaactaaa aatggacgtc agtatgaagt
    17461 gaaaaagttt gttgaaaagc cagcacaagg tacggcacca tcaaacctag ctattatggg
    17521 gcgatatgta ttaacgcctg aaattttcga ttatttaaaa acgcaaaaag aaggcgcagg
    17581 taacgaaatt cagttaacag atgcgattga gcgtatgaat aatgataacc aagtgtatgc
    17641 atatgacttc gaaggcgaac gttatgacgt tggagaaaaa ttaggcttcg ttaaaactac
    17701 catagaatat gcattgaaag atgacagtat gcgagaagaa ttaacacgat ttattaaaga
    17761 attaggttta taacattaaa aagcataaat gattctatat taatgcaact ttgtattcgt
    17821 acaaggttgt atttttttcg caaacaattg cttgctgaaa gcgcaccatt aaatctaacc
    17881 aacacactat cacaaacatt tatggatatt ttgtaatttt ataaaagcaa acgtagaata
    17941 atgcgataag taataatgct atcgttaaaa ataagaatga caatgcaata aaatcatatt
    18001 cgaagcatag aattgcagac attaaaaata ctgcactcaa actgatacat agcgcaaata
    18061 tatatgcaat ttgggtcttg tgtttatctg caatggtcgt ttgatttata aaagagatta
    18121 gtgtgaaatc tttagttatt gcgacataaa tgcctaatat aaccatactg atacagaaca
    18181 cgatagaaaa tacgatgttg acggtcattg ttgcaccttc ccgatagaat ataatgtact
    18241 gtcttttagt gattgatgcg tttcttatat atgtatgttt ctaaaaaaca gtacgttttt
    18301 atcaattttt aatttattag ttgaatgaat ggggttatga aaaaggaagt tttaatcttt
    18361 tgtcatgaaa taaatgggat gaatatcacg actagaagta atgttacgaa caggagcgta
    18421 taaatctaga gacgctaaat tcgacatagt atgttgctca attatggctg atgatgaatt
    18481 taaagtatgt gcgttggaac tgtcggattt ttgttcataa tgttttgcat attgtgccat
    18541 gatgaatagt gtaaaaataa ataaaacaat aagagatata atgcccataa tcaaaagtat
    18601 ttgtttagag cctttcatta tttcacatcc tttctaaaat atatttgtaa ctaaatttaa
    18661 aatagttatt tttgtaattc taaacctttt tcatcgcgaa aacaattaaa
//
LOCUS       DAKSQE010000050        18357 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.050, whole genome shotgun sequence.
ACCESSION   DAKSQE010000050 DAKSQE010000000
VERSION     DAKSQE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18357
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.050"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            157..465
                     /gene="spn"
                     /locus_tag="O0V33_001432"
     CDS             157..465
                     /gene="spn"
                     /locus_tag="O0V33_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HCX8976064.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYGNEADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
     gene            complement(571..2058)
                     /locus_tag="O0V33_001433"
     CDS             complement(571..2058)
                     /locus_tag="O0V33_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HCX8976065.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASEANEKPSTNQE
                     SKVVSQTEQNSKETKTVESNKNFVKLDTIKPGAQKITGTTLPNHYVLLTVDGKSADSV
                     ENGGLGFVEANDKGEFEYPLNNRKIVHNQEIEVSSSSPDLGEDEEDEEVEEASTDKAG
                     VEEESTEAKVTYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKGHTSVKGK
                     VALSINNKFINFEESVKGGVSKEDTKASSDGIWMPIDDKGYFNFDFKTKRFDNLELKE
                     GNDISLTFAPDDEEDALKPLIFKTKVTSLEDIDKAETKYDHTKLNKVKVLDNVKEDLH
                     VDEIYGSLYHTDKGKGILDKEGTKVIKGKTKFANAVVKVDSELGEAQLFPDLQVNEKG
                     EFSFDSHGAGFRLQNGEKLNFTVVDPITGDLLSNEFVSKEIDIEETPEQKADREFDEK
                     LENTPAYYKLYGDKIVGFDTNDFPITWFYPLGEKKVERTTPKLEK"
     gene            complement(2077..2775)
                     /locus_tag="O0V33_001434"
     CDS             complement(2077..2775)
                     /locus_tag="O0V33_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL11"
                     /protein_id="HCX8976066.1"
                     /translation="MKLKNIAKASLALGILTTGMITTTAQPVKASEQSRLSVTSNDTQ
                     ELKKYYSGTGYNFQNVSGYREKDKMNIIDGTQLNVVTLLGTDKERFKDYDYDYEGLDV
                     FVVREGSGKQAENISIGGITKTNKNDYKDFVNNVGLEITKPTGHNTATRQAETYRINK
                     EEISLKELDFKLRKHLIENHELYKTEPKDGKIRITMKGGGYYTFELNKKLQPHRMGDV
                     IDGRNIEKIEVDLY"
     gene            complement(3158..4390)
                     /locus_tag="O0V33_001435"
     CDS             complement(3158..4390)
                     /locus_tag="O0V33_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCX8976067.1"
                     /translation="MSNTQTKNVPELRFPGFEGEWEEKKVGELLEFKNGLNKGKEYFG
                     SGSSIVNFKDVFNNRSLNTNNLTGKVNVNSKELKNYSVEKGDVFFTRTSEVIGEIGYP
                     SVILNDPENTVFSGFVLRGRPKSGIDLINNNFKRYVFFTNSFRKEMITKSSMTTRALT
                     SGSAINKMKVIYPVSAKEQRKIGDFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQE
                     LRFKDENSEDYPHWENSKIEKYLKERNERSDKGQMLSVTINSGIIKFSELDRKDNSSK
                     DKSNYKVVRKNDIAYNSMRMWQGASGRSNYNGIVSPAYTVLYPTQNTSSLFIGYKFKT
                     HRMIHKFKINSQGLTSDTWNLKYKQLKNINIDIPVLEEQEKIGDFFKKMDILISKQKI
                     KIEILEKEKQSFLQKMFL"
     gene            complement(4383..5939)
                     /locus_tag="O0V33_001436"
     CDS             complement(4383..5939)
                     /locus_tag="O0V33_001436"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCX8976068.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALAGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVT
                     DGKDKLRHVYDPTCGSGSLLLRVGKEAKVYRYFGQERNNTTYNLARMNMLLHDVRYEN
                     FDIRNDDTLENPAFLGHTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADF
                     AFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGIIRRYLIEEKNYLEAVIGLPANIFYG
                     TSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDK
                     YSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAY
                     LKELGVLKDE"
     gene            5999..6118
                     /locus_tag="O0V33_001437"
     CDS             5999..6118
                     /locus_tag="O0V33_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976069.1"
                     /translation="MWWCRDVENFDVDGGNFIFMEIGIGFITRKQKRLNDDID"
     gene            complement(6201..6884)
                     /locus_tag="O0V33_001438"
     CDS             complement(6201..6884)
                     /locus_tag="O0V33_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HCX8976070.1"
                     /translation="MKLTALAKVTLALGILTTGTLTTEAHSGHAKQNQKSVNKHDKEA
                     LHRYYTGNFKEMKNINALRHGKNNLRFKYRGMKTQVLLPGDEYRKYQQRRHTGLDVFF
                     VQERRDKHDISYTVGGVTKTNKTSGFVSTPRLNVTKEKGEDAFVKGYPYDIKKEEISL
                     KELDFKLRKHLIEKYGLYKTLSKDGRIKISLKDGSFYNLDLRTKLKFKHMGEVIDSKQ
                     IKDIEVNLK"
     gene            complement(7247..7945)
                     /locus_tag="O0V33_001439"
     CDS             complement(7247..7945)
                     /locus_tag="O0V33_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HCX8976071.1"
                     /translation="MKLTAIAKATLALGILTTGVMTAESQTVNAKVKLDETQRKYYIN
                     MLKDYYSQESYESTNISVKSEDYYGSNVLNFNQRNKNFKVFLIGDDRNKYKELTHGRD
                     VFAVPELIDTKGGIYSVGGITKKNVRSVFGYVSHPGLQVKKVDPKDGFSIKGLFFIQK
                     EEVSLKELDFKIRKMLVEKYRLYKGASDKGRIVINMKDEKKHEIDLSEKLSFDRMFDV
                     LDSKQIKNIEVNLN"
     gene            complement(8286..8981)
                     /locus_tag="O0V33_001440"
     CDS             complement(8286..8981)
                     /locus_tag="O0V33_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HCX8976072.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVQAAEKQERVQHLHDIR
                     DLHRYYSSESFEYSNVSGKVENYNGSNVVRFNPKDQNHQLFLLGKDKEQYKEGLQGQN
                     VFVVQELIDPNGRLSTVGGVTKKNNKTSETNTPLFVNKVNGEDLDASIDSFLIQKEEI
                     SLKELDFKIRQQLVNNYGLYKGTSKYGKIIINLKDENKVEIDLGDKLQFERMGDVLNS
                     KDIRGISVTINQI"
     gene            complement(9428..10132)
                     /locus_tag="O0V33_001441"
     CDS             complement(9428..10132)
                     /locus_tag="O0V33_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HCX8976073.1"
                     /translation="MKMTAIAKASLALSILATGVITSTAQTVNASEHESKYENVTKDI
                     FDLRDYYSRASKELKNVTGYRYSKGGKHYLIFDKNRKFTRIQIFGKDIERIKKRKNPG
                     LDIFVVKEAENRNGTVYSYGGVTKKNQGAYYDYLSAPRFVIKKEVGAGVSVHVKRYYI
                     YKEEISLKELDFKLRQYLIQDFDLYKKFPKASKIKVTMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
     gene            complement(10493..11470)
                     /locus_tag="O0V33_001442"
     CDS             complement(10493..11470)
                     /locus_tag="O0V33_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL4"
                     /protein_id="HCX8976074.1"
                     /translation="MKMTQIAKTSLALSLLTTGASIVTTQSANAEVASALKTEQAIKS
                     KIQKVTTSPSTKVKATQPTPSVTTTTPPSPNVQPQSPQPTPSVTTPPSPNVQPQSPQP
                     TPNPTTPHSSNVETKQPQSPTTKQAQKEINPKYKDLRTYYTKPSLEFEKQFGFMLKPW
                     TTVRFMNVIPDWFIYKIALVGKDDKKYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITK
                     TNSKKVDHKAELSITKKDEKGKISHDDSEYKITKEEISLKELDFKLRKQLIEQHNLYG
                     NIGSGTIVIKTKNGGKYTFELHKKLQEHRMADVIDGTSIERIEVNLKSS"
     gene            complement(11817..12848)
                     /locus_tag="O0V33_001443"
     CDS             complement(11817..12848)
                     /locus_tag="O0V33_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HCX8976075.1"
                     /translation="MKIRTIAKASIALGLVTTGASIVTTQSANAEVASALKAGQLAKI
                     NVSTSAITTTAQAVNAEQNHTANPEQAAKSNTENVSTSLSTQTEQTPKSNIQKATQPA
                     PSGTTQSLPNAQPQSTQPTPSVTTPPSSNVETPQPTSPTTKQAQKEINPKFKGLRSYY
                     TKSSLEFKNELGIIIKKWTTIRFMNIVPDYFIYKIALVGKDDKKYGEGVHRHVDVFIV
                     LEQNKYGVDKYSVGGITKANRKKVDYKTGISITKEDKKGTISHDVSEYKITKEEISLK
                     ELDFKLRKQLIEQHNLYGNIGSGTIVIKMKNGGKYTFELHKKLQQHRMADVIDGTNID
                     RIEVNLKSS"
     gene            complement(13136..13837)
                     /locus_tag="O0V33_001444"
     CDS             complement(13136..13837)
                     /locus_tag="O0V33_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HCX8976076.1"
                     /translation="MKMKSIAKVSLVLGILATGVNTVTEQPVHAENKHVQVSQNSKNL
                     KAYYTQPSVEYKNVTGYISSIQPKPGTKFLNMIEGNTVNNLALVGKDKEHYHTGVHRN
                     LDIFYVNEDKRFEGAKYSIGGITKASDKVVDQVAEARVIKEDHTGEYDYDFFPFKIDK
                     EAMTLKEVDFKIRKHLIDHYGLYGEMSSGTVTVQMKYYGKYTIELDKKLQEDRMADIV
                     KVIDIDRIEVKVKKA"
     gene            complement(14120..14800)
                     /locus_tag="O0V33_001445"
     CDS             complement(14120..14800)
                     /locus_tag="O0V33_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HCX8976077.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNIQSVQAKTEVKQQSEADLKL
                     YYNGPSFEYKKVTGYGFIEGKDRFIDFIYNGQYNKISLVGSDKDKYNEEVNPDIDVFV
                     VREGNGRQADNHSIGGVTKTNRGVYYDYIHSPILEIKKGNEEPQNSLYQIYKENISLK
                     ELDYRLRERAIKQHGLYSNGLKQGQITITMKDGKSHTIDLSQKLEKERMGDSIDGRQI
                     QKILVEMK"
     gene            15272..16117
                     /locus_tag="O0V33_001446"
     CDS             15272..16117
                     /locus_tag="O0V33_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCX8976078.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPEDWRG
                     KVSVRQLDYFNQESMVKAFKGIDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYASRLLATSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYDAGARGLLDQESNEFQQLVNDQPQTLQSF
                     LQENI"
     gene            16136..16495
                     /locus_tag="O0V33_001447"
     CDS             16136..16495
                     /locus_tag="O0V33_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HCX8976079.1"
                     /translation="MNIISTILIIFVALEFFYIMYLETIATTSRKTSETFNISVDKLK
                     DKNINVLLKNQGVYNGLIGVLLIYGLFISSNPKEICAAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            17017..17169
                     /locus_tag="O0V33_001448"
     CDS             17017..17169
                     /locus_tag="O0V33_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976080.1"
                     /translation="MVPEEKGSITLSKDAATIFATAKFKPFQNRIKNNPQKTNPFLKL
                     HENKKS"
     gene            17427..17753
                     /locus_tag="O0V33_001449"
     CDS             17427..17753
                     /locus_tag="O0V33_001449"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCX8976081.1"
                     /translation="MTTRERRSFSSEFKLQKVRLYENGKPKNEIIREYDLTTSTFSNP
                     IKQHQNTGSFNHQDNLKSDEKELIKLRKEVQHLKMENDVLKQILLITRRNRNHLTECV
                     SIFNIH"
BASE COUNT         5444 a         3262 c         2593 g         7058 t
ORIGIN      
        1 ctaaggaaca caagtatcgc actaaagatt aattgcattt aaacaatatt tgtcacttta
       61 actatttatt aaaaatcatt aaatcgactt aataaacgta attttctcaa tattcagtgt
      121 tatgatggag ttacaatttt tagaggagtg atagatatga aatttaaaaa ggttttagta
      181 gctacggcaa tggtaggtgt attagcaact ggcgttgttg gatatggtaa tgaagcagat
      241 gcgaaagtgt attctcaaaa tggactcgta ctacatgatg atgcaaactt cttagaacat
      301 gaattaagct atatcgatgt gttattagat aagaatgctg accaagctac aaaagacaac
      361 ttaagatcat acttcgccga taaaggacta cattcaatca aagacattat caacaaagct
      421 aagcaagacg gctttgatgt ttcaaaatat gaacacgtaa aataaattca ataatgcttt
      481 ataacaaagc acataaaaaa acagctcaat gacgtagaag tcagtgatta attcactaat
      541 ttcattacat cattaagctg ttttatttaa ttatttttct aatttaggtg ttgtacgttc
      601 aactttcttt tcacccaatg gatagaacca agtaatcggg aaatcgttag tatcgaatcc
      661 aactatttta tcgccgtata acttgtagta agcaggcgta ttttcaagtt tttcgtcaaa
      721 ctcacgatcc gctttttgtt caggtgtttc ttcaatatca atctctttag aaacaaactc
      781 attacttaac aagtcacctg taataggatc aaccactgtg aagtttaatt tttctccatt
      841 ttgtaatcta aaaccagcac catgtgagtc aaagctaaat tcaccttttt catttacttg
      901 taaatcaggg aataattgtg cttcacctag ttcagagtct accttcacta ctgcattcgc
      961 gaatttagtc tttcctttaa ttactttagt accttcttta tcaagaatac ctttaccttt
     1021 gtctgtatga tataagcttc catatatttc atcaacatgt aaatcttctt taacattatc
     1081 taaaactttc actttgttga gtttagtatg gtcatattta gtttctgctt tatcgatatc
     1141 ttctaagctc gttactttag ttttgaaaat taaaggtttt aatgcatctt cttcatcatc
     1201 aggtgcaaat gttagtgaaa tgtcattacc ttcttttaac tctaaattat cgaaacgttt
     1261 cgttttgaag tcaaagttaa agtatccttt gtcatcaata ggcatccaga taccatctga
     1321 actagcttta gtgtcttctt tactaactcc gcccttaacg ctctcttcaa aattaataaa
     1381 tttattatta atagataaag caactttacc ttttacagac gtatgccctt taataatacc
     1441 tgatccttca gtaataggtt cgataaaaac ttggtgatgt ccatcttttt cttttagttg
     1501 ttctttcggt atttcatacg ctttttcata tcgcggtgtt gtgtaagtaa ctttagcttc
     1561 tgtactttct tcctcaacgc cagctttatc agttgaagct tcttccacct cttcatcttc
     1621 ttcatcttca cctaaatcag ggcttgacga cgaaacctca atttcttgat tatgaacaat
     1681 tttacgatta tttaaagggt actcaaattc tcctttgtca tttgcttcaa caaaacccaa
     1741 accgccattt tctactgaat ccgcactttt cccatcaact gttaataaaa catagtgatt
     1801 tggtaaagta gttcccgtta tcttttgagc tccaggttta atagtatcta atttaacaaa
     1861 gttcttatta gattctactg tttttgtttc ttttgaattt tgttcagtct gtgaaacaac
     1921 ttttgattct tgatttgtac ttggcttctc attagcctct gatgcttctg cttgtccgtt
     1981 tgtcatgata tataacattg taattgcaac agatactaac ccgactttca ttttacgtaa
     2041 cttaaaattt tccctcatga tatactccct cgaatattaa tataaatcga cttcaatttt
     2101 ttctatattt ctaccatcaa ttacatcacc catacgatga ggctgtaatt ttttatttaa
     2161 ttcaaaagta tagtagccgc cacctttcat agtaattcta attttaccgt ctttaggctc
     2221 tgtcttataa agttcatgat tttcaattaa atgttttctt aatttgaaat ctaattcttt
     2281 taatgaaatt tcttctttat taattctata agtttctgct tgtcttgttg ctgtattatg
     2341 tcctgttggt ttagttattt ctagacctac attatttacg aaatctttat aatcgttttt
     2401 attcgtcttg gtaattccac ctattgaaat attttcagct tgtttacctg atccttctct
     2461 gactacaaag acatctaacc cttcataatc atagtcataa tctttaaatc tttctttgtc
     2521 tgtaccaagt aaagtaacta cattaagttg tgtcccatca ataatgttca ttttatcctt
     2581 ttctctataa ccactcacat tttgaaaatt atatcctgtt ccactgtagt atttttttaa
     2641 ttcttgcgtg tcgtttgaag taactgataa tctgctttgc tcacttgctt ttactggctg
     2701 agcagtagtt gtaatcatcc ctgttgtcaa aatccctagt gctaaacttg ctttagcaat
     2761 attttttaat ttcataattc tatgctccca atttttagtc tatttgattt attctattac
     2821 gcaatacgaa caatcctcat tcattataat gagcaacggt cgtgcttcac attaaactta
     2881 ctttaactaa aaattaatca ttattaagtg atttaataaa tagttaacta atatcattca
     2941 attcctatcg atctactctc ttttatttac gaataacact ttatctcaac ttaatcttta
     3001 tttaactact tattctgtac acaatttcga cacaaaaaag acactgtgct atcacagtgc
     3061 ctaaatttaa tgttcgtttt taatttactt tatgtttata acttttatgt taaattacac
     3121 aaattttatt cttatgcaac ctatgtgttt atcaaagtta taagaacatc ttttgtaaaa
     3181 aggattgttt ctctttttct aatatttcaa tttttatttt ctgtttacta atcaatatat
     3241 ccattttttt aaagaaatca cctatctttt cttgttcctc caatacaggt atatctatat
     3301 ttatattttt taattgttta tattttaagt tccatgtatc tgatgttaat ccttgtgaat
     3361 taattttaaa tttatgaatc attctatgtg ttttaaactt atatccaata aataatgagc
     3421 tagtattttg tgttggataa agcacagtat atgcagggct aacaatccca ttataatttg
     3481 atctaccact agccccttgc cacattctca tagaattata tgcaatatca tttttcctaa
     3541 ctactttata attactttta tctttacttg aattatcttt tctatccaat tcactaaatt
     3601 ttataatgcc actatttata gttactgaaa gcatttgacc tttgtcagaa cgttcgtttc
     3661 tctcttttaa atatttttct attttgctat tttcccaatg tggataatct tcactattct
     3721 catctttgaa tcgcagttcc tgtgagaaga ttttctgcat atagcctttt ttttgttgtt
     3781 gaagtaattc aagcttttgt tcttctaatt caatttgtcg gtcgagtttg ctgaagaagt
     3841 cacctatttt tctctgttct ttagccgaaa cagggtatat gaccttcatt ttattaattg
     3901 cgctacctga tgttaaagct ctagttgtca tagaactttt tgtaatcatt tcttttctaa
     3961 atgaattagt aaaaaagaca tatcttttaa aattattatt tattaaatca attcctgatt
     4021 taggccgccc tcttaataca aatccactaa acacagtatt ttcagggtca tttaaaatta
     4081 cagacggata acctatttca ccaattacct cactagtcct tgtaaaaaaa acatcaccct
     4141 tttcaacaga ataatttttt agttctttgc tattcacatt aacttttcca gtcagattat
     4201 ttgtatttaa gctcctgtta ttaaatacat ctttgaagtt aacaatcgac gatcctgagc
     4261 caaaatattc ttttccttta tttaaaccat ttttaaattc taataactcg ccaaccttct
     4321 tctcttccca ttcgccttca aaccctggga atctcaactc tggcacattt ttcgtttgtg
     4381 tattactcat ctttcaacac cccaagttct ttcaggtatg cattgatttc ttgttcaatt
     4441 tctgcgattt ctttatcgat atttttcaaa tcttgttgga cttgatctaa atcaattggc
     4501 gcttcttctt cgaatgtatc gacatatctc ggtatgttta agttgtaatc gttatcggcg
     4561 atctcttgta atgtcgcgct gtagctatac ttatcaattg tttccttacg cttatatgtg
     4621 tctataattc gttcgacttg ggcatcgctt aaatggtttt ggttttttcc tttttcaaaa
     4681 tcattggatg catcgataaa taatacgttg tcgtcttgtt ggcgacattt tttaaatact
     4741 aagatacatg ttggaatact tgtcccatag aaaatgttcg ctggcaaacc aatcacggct
     4801 tctaagtagt tcttttcttc aattaaataa cgacgaatga taccttctgc ggcaccacgg
     4861 aataagacac catgtgggag tacaacggcc atggtacctt catcatctag gtaatgtacc
     4921 atgtgttgaa taaaggcaaa gtctgcttta gactttggcg caagcttgcc gtatccgctg
     4981 aagcgttcgt cattttcaaa ttttgagtct gctgtccatt tcgcgctata tggtgggttc
     5041 gcaataaccg catcaaatgt atgtcctaaa aaggctggat tttccaacgt atcatcatta
     5101 cggatatcga aattttcata tcgcacatca tgtaacaaca tgttcatgcg cgctaagttg
     5161 tatgtggtat tgttacgttc ttgtccaaaa taacgataca cttttgcctc tttaccaacg
     5221 cgtaacagta atgaacctga accacatgtt gggtcatata catgacgtaa tttatcttta
     5281 ccgtctgtga caatcttcgc cagtatctta gatacttgtt gtggtgtata gaactcgcct
     5341 gcttttttac ccgctgtcgc cgcaaagcgc ccgataagaa attcgtatgc atcacctaac
     5401 atatcaattt ccatatcact gtgaacgaat ggtaagtcat caaggttaac cataactttg
     5461 gaaattaacg cagtacgttc tttgacattg ttacctaaac gcgttgaact taagtccata
     5521 tcgctgaaca gtccgataaa gtcattttca ctttcttcac ctagtgttga agtttcaact
     5581 ttacgaattg ccgtcgccag atgttcgata tcgaaatctt gcgtttcaat ttcacgaatc
     5641 atcgcactga ataaatcttg tggctcaatg aagtaaccaa cttgatcaat taattctgct
     5701 tttaagtctt cacggtattc ttcatctgcc catgcttctt gatatgtaat atcttcacct
     5761 gccaaggcat ctgcatattc ttgttccgct ttttcagata agaagcgata gaaaatcaag
     5821 cctaaaatgt aattacggaa ttcactcgca tccatattcc ctcttaaatc attcgcaatt
     5881 gaccataatt ttttatgtaa ttcagcttgt tgctgacgtt gtttttcagt aatagacatg
     5941 tgattcctcc gcctttgcat aagtaatttg tctctttgtg taatagattt attataacat
     6001 ttggtggtgt cgcgatgttg agaattttga tgttgatggt ggaaatttta tattcatgga
     6061 gattgggata ggattcataa cacgcaaaca aaaacggctt aacgacgata tcgattgatg
     6121 catccttttg gtaaggtata cgcaccaatt tacttttcat cgttaagccg cttcaattac
     6181 tttttattat tcataatgat ttactttaaa ttcacttcaa tatccttaat ttgtttacta
     6241 tctatgactt cccccatatg tttgaatttt aatttagttc ttaaatcaag gttataaaag
     6301 ctaccatctt tcagactaat tttaatccta ccatcttttg agagtgtttt ataaagaccg
     6361 tatttttcaa ttagatgctt tctcaactta aaatctaact ctttcaatga tatttcttct
     6421 tttttaatat cataagggta acctttcaca aaagcatctt cacccttttc ttttgtaaca
     6481 tttaatcttg gtgtgctgac aaagccggat gttttattgg tctttgttac accaccaaca
     6541 gtatatgata tgtcgtgctt gtctcttctt tcttgaacaa aaaacacatc taagcccgta
     6601 tgtcttcgct gttgatattt acggtactca tctccaggca ataatacttg agtcttcatc
     6661 cctctatatt taaaacgtaa gttattttta ccatgtctca aagcattaat gtttttcatt
     6721 tccttaaagt ttccagtgta gtatcggtgt aatgcttctt tgtcatgttt gtttactgac
     6781 ttttgatttt gtttagcatg acctgaatgg gcctctgttg ttaaagttcc tgttgttaaa
     6841 atccctaatg ctaatgttac ttttgctaat gctgtcaatt tcatatttat ttactccaat
     6901 cttaatgtat tggattgtta ttattacgta atttgaatca tatacgctta ttataacgtc
     6961 catcgtttaa cgcgataatt aacctcgatt taaatatgcg tgaattatta ttaattaaaa
     7021 acaaatatat tttaatgtcc aggttaatta actttcacgc tttacatgtc gttgtttttc
     7081 cggatcatcg ttttctacaa ctaattgtgt tatttctact ctttgaaacg caaaaaacaa
     7141 cttaacaaca ataatgtatt taacacagtt cttattcaaa ggtacgggtg tcaatgcaca
     7201 ttttatcgtt aagccgcttt ttaatttttt atactatcat ctttaattaa ttcaaattca
     7261 cttcaatatt tttaatctgc ttactatcca gcacatcaaa catacgatca aaacttaatt
     7321 tttcacttaa atcaatttca tgtttctttt cgtctttcat attgataaca attctacctt
     7381 tatctgacgc gcctttatac aatctatatt tttcgactaa catttttctg attttaaaat
     7441 caagttcttt caatgatact tcttcttttt gaataaagaa caaccctttt atcgaaaagc
     7501 catctttagg atcaactttt ttaacttgca gtcccggatg acttacatag ccaaacacgg
     7561 atctcacatt tttctttgtt ataccgccaa cgctatatat gccgccttta gtatctatta
     7621 attcaggtac tgcaaagaca tcacggccat gcgtcagttc tttatattta tttctatcgt
     7681 cgccaattag aaatactttg aaatttttat ttcgttggtt aaagtttaaa acgttagacc
     7741 cataataatc ttcactttta acactaatgt ttgttgattc atagctttct tgagaatagt
     7801 aatcttttag catatttata taatatttgc gttgtgtttc atccaacttt actttcgcgt
     7861 ttacagtttg actttctgct gtcatcacac ctgttgttaa tattcctaat gctaatgttg
     7921 ctttagctat tgctgttaat ttcataattt attgctccaa tcttaatgta ttggattgtt
     7981 atgattacgt aatttgaaac atctgccttt attataaagt gcatcgaaac aaactgacat
     8041 taacgtgcgt ttaaataact ttgaatcatt gttaatgaac acctaattaa tattcaaagt
     8101 acgtggtttt aatgagtggt atgacttatt tagtatactc gtgacttgtg ttatcaaatg
     8161 taaaaaacag cctaacgacg acgttgcttt aaatacgctc ctttgatagg atatacatat
     8221 ttattcaacg tttcatcgtt aagctgcctc aaaatttatt actttaaagt cattgattgc
     8281 ttactttaaa tttggttaat agtgactgat atacctctaa tgtctttact attcaacaca
     8341 tcgcccatgc gctcgaattg taatttatca cctaaatcaa tttctacttt attttcgtct
     8401 ttcaaattga taatgatttt accgtattta gatgtacctt tatataatcc gtaattatta
     8461 actaattgtt gtctaatttt gaaatcaagc tcttttaatg agatttcttc tttttggatt
     8521 aaaaatgagt caattgatgc atctaaatct tcaccattaa ctttattaac aaataaaggt
     8581 gtattagttt cagaagtttt gttgtttttc ttcgttacac caccaacagt agatagtctg
     8641 ccgtttggat caattaattc ttgtactaca aagacatttt ggccttgtag accttcttta
     8701 tattgttctt tatcttttcc taataagaat aattggtgat tttgatcttt tgggttaaag
     8761 cgtacaacgt tagaaccatt gtagttttca accttaccac taacattact atattcgaaa
     8821 ctttctgatg agtagtatcg atgtaaatct ctaatatcat gtaaatgttg tactctctct
     8881 tgtttttctg ccgcttgaac tgcttgacct tctgatgtaa tgacaccagt agttaataaa
     8941 cctaatgcta atgttgcttt agctaacgtt tttaatttca tagtattatt ctcccaatct
     9001 atttttaaat tttgacttaa tatatgttta tgtgattaat tcaattacgt aatacgaaca
     9061 atctactatc attatataag ccggatactg cgctatgaat taaccgcttt ttaactattc
     9121 ctgaaccaat gttaagctaa ttaatggaat cctaacagta ttaatcattt tttataactc
     9181 tatgctattt ttttcaccat tcacactttc tttcaattta attatgtttt ccacattgtt
     9241 tcatgtcact agtgcgacac cgtgcgacta taggtatcaa ctattttcac agtttttatt
     9301 gtcgttatta tctttcatcg acccacttca tttccgatcg taacgacaca aaaaagaagc
     9361 taagcaacct atgctgccta gctcatctat actatccata ttttactatt atccatgttt
     9421 cgttgaatta tctaatattg gcttctattt tctcaatatt tctgccatct atgacgtcac
     9481 tcattcggtt tgtttgtaac tttttattga gttcaaacgt ataatagccg ccatctttca
     9541 ttgtcacttt tatcttgcta gctttaggaa actttttata cagatcaaaa tcttgaatta
     9601 aatactgtcg taatttaaaa tcaagttctt tcagcgaaat ctcttcctta taaatgtagt
     9661 atcttttaac atgcacagaa acacctgcac ctacttcttt tttaataacg aatctaggtg
     9721 cgcttaagta atcatagtaa gcgccttgat ttttcttagt aacgccacca tatgaataca
     9781 ctgtgccgtt gcgattttcc gcttctttaa ctacaaatat atctaatcct ggatttttac
     9841 gttttttaat cctttctata tctttaccaa aaatttgtat tcttgtgaac tttctatttt
     9901 tatcaaagat aaggtagtgc ttgccacctt tgctataacg atagccagta acatttttaa
     9961 gttccttact tgcgcgacta tagtaatctc tcaagtcgaa gatatctttt gtcacatttt
    10021 catattttga ttcatgttcg ctcgcattta cagtttgagc cgttgatgtt ataaccccag
    10081 tcgctaaaat acttagagct aaactggctt tcgcaattgc tgtcatcttc atagttgtat
    10141 gctccaatcg taattattag atttgttcga ttacgtttat tgaatcatac agctttatta
    10201 tagttggcgt atgtctctat tcatattaaa ccttgtttaa ctatatttga atcatcgtta
    10261 attaaattaa ggaatccata atgttcgtta aataaaaatg acttttgttg tacttcaacg
    10321 cttgatagat tggtattttt agacacattt aggacataag catgttatgt gtggtcaaca
    10381 tcaaatattc gtcttcttat accactgtgc aacacgaaag aagctgagca acatgtaaat
    10441 ttttgtcact caacttctcg gtacatcgtt caacggttct atgcagtatc catcacgatg
    10501 attttagatt cacttcaatt ctttcgatac tagtgccatc tatgacatct gccatgcgat
    10561 gttcttgtaa ttttttatgc aattcaaacg tgtacttccc accgtttttc gttttaataa
    10621 caattgttcc tgaaccaata ttaccgtaca aattatgttg ttcaatgagt tgctttctca
    10681 atttaaaatc aagctctttc aaggaaatct cttctttagt tatcttatat tctgaatcat
    10741 cgtgtgagat tttgcctttc tcatcttttt tagtaatgct taattctgct ttgtgatcaa
    10801 cttttttact atttgtcttc gtgatacccc cgacagaata ttttttcaat tgatatttat
    10861 tgtcctctaa aacgataaat acatcaatat tatcgtatgg cccatcttta tatttcttat
    10921 catcttttcc aactaaagct attttataaa tgaaccaatc tggtataaca ttcataaacc
    10981 taactgtcgt ccatggtttc agcataaagc caaattgctt ttcgaattct aaactcggtt
    11041 tcgtataata tgttcttaaa tccttatatt tagggtttat ttccttttgt gcttgttttg
    11101 tggttggcga ttgcggttgt tttgtttcga cattagatga atgcggtgtt gttgggtttg
    11161 gtgttggttg tggcgattgc ggttgtacat ttggtgaagg cggagttgtt acacttggtg
    11221 ttggttgtgg cgattgcggt tgtacatttg gtgaaggcgg agttgttgta gttacgcttg
    11281 gtgttggttg tgttgctttt acttttgttg aaggcgatgt tgttactttt tgtattttag
    11341 attttattgc ctgttctgtt ttaagtgctg atgctacttc tgcattagcc gattgcgttg
    11401 ttactatact tgcacctgtt gttaaaagtg atagtgctaa actggttttc gcaatttgtg
    11461 tcattttcat agttgtatgc tccaatctat tatattcgat tgttctttac gtaattagaa
    11521 tcatacaact acattataga agacgtatcg gtctattcac attaaaccat gtttaactat
    11581 atttgaatca tcgttaagta atccataatt ttcgttaaat acagatggtt tcgaagcgat
    11641 tcaacgcttg cacattgggc attgcatcat tttcaagaca ttgagatgtc acttttacaa
    11701 acaagtgaat aatttccctt aacatcatta cgcaaaacaa aagaagctaa gcaatgatgt
    11761 aggtcattgt cgcttaactt ctcatttcat cgttcaacgg ttctatgtag tatccatcac
    11821 gatgatttta gattcacttc aattctatca atatttgtac catcaataac atctgccatg
    11881 cgatgttgtt gcaatttttt gtgtaattca aacgtgtact ttccaccatt tttcatttta
    11941 ataacgattg ttcctgaacc aatattaccg tacaaattat gttgttcaat gagttgtttt
    12001 ctcaatttaa aatcaagttc tttcaaggaa atctcttctt tagtaatctt gtattctgaa
    12061 acatcatgtg agattgtacc ttttttatct tctttagtaa tacttattcc agttttgtaa
    12121 tcaactttct ttctatttgc ttttgtgata ccaccgaccg agtatttgtc tacgccatat
    12181 ttattttgtt ctaaaacgat aaatacatcg acatgcctat gcacgccttc accatatttc
    12241 ttatcatctt ttccaactaa agctatttta tatatgaaat aatctggaac aatattcata
    12301 aatcttattg ttgtccattt tttgataatt atacccaact catttttaaa ttctaaactt
    12361 gatttcgtat aatatgatct taaaccttta aatttagggt ttatttcctt ttgtgcttgt
    12421 tttgtggttg gcgatgttgg ttgtggtgtt tcgacattag atgaaggcgg tgttgttaca
    12481 cttggtgttg gttgtgttga ttgcggttgt gcatttggta aagactgagt tgttccgctt
    12541 ggtgctggtt gtgttgcttt ttgtatgtta gattttggcg tctgttctgt ttgagttgaa
    12601 agcgatgttg atacattttc tgtgttagac tttgccgcct gttcaggatt agccgtatga
    12661 ttttgttctg cgttcactgc ttgcgctgtc gttgtgattg cgcttgttga tacgtttatc
    12721 tttgctaact gtcctgcttt aagtgctgat gctacttctg cattggccga ttgcgttgtt
    12781 actatacttg cacctgttgt tacaagccct aacgctatac tcgctttcgc aattgttctt
    12841 attttcatag ttgtatgctc caatcgtaat tattcgattg ttctttacgt aattagaatc
    12901 atacaacttc attatagagg acatattggt ctattcacat taaccctcgt ttaacaattg
    12961 ttgaaatatt attaagtcat ttaagcaaaa aataatgagt actttcgagg tttatgtggt
    13021 gttactatgt atctttgaaa tgattttaag acataaaaaa gaagttaagt aacgttttat
    13081 catcacttaa cctcactatt gcaatttcaa ttcattacga cgaccagtct aagcattatg
    13141 cttttttaac tttgacttca atcctatcaa tatctatgac tttgacgata tcggccattc
    13201 ggtcttcttg taacttttta tccaattcaa ttgtatactt gccatagtat ttcatttgga
    13261 cagtaactgt tcctgaactc atttcaccat aaagaccata atgatcaata agatgttttc
    13321 ttattttaaa atcaacctct tttaatgtca ttgcttcttt atctatttta aatgggaaaa
    13381 agtcataatc atattcacca gtgtgatctt ctttaatgac tcttgcttct gctacttggt
    13441 cgacaacttt atcgcttgct tttgtgatac caccaatcga gtactttgca ccttcaaatc
    13501 tcttatcctc attaacataa aatatatcaa ggttgcgatg tacacccgta tgatagtgtt
    13561 ccttatcttt gccaactaaa gctagattat taactgtatt accttctatc atattcaaaa
    13621 atttagtgcc tggtttaggt tgaatactac tgatataacc tgtcacattt ttatattcaa
    13681 cactaggttg agtatagtac gcttttaaat ttttactatt ttggcttact tgaacatgtt
    13741 tattttcggc atgcaccggt tgttccgtta cagtatttac acctgtagct aaaataccca
    13801 acactaaact tacttttgca attgatttca ttttcataat tgtatgctcc aatatattag
    13861 attagattgt tttattacgt aaattgaatc atacaaccat attataggag ctgtatgctg
    13921 atattcacat taaccctttt ttaactattc ataaaatatg attaagctag ttaagtaaaa
    13981 gataatgcgt gctgcgactg tttgtgtgag ttttgtaact ttgccctcaa aaaagaagct
    14041 aagcaacagt acgtcgctta acttccatat atttccataa caagcacgat tcaacatagc
    14101 gctttgtcgt tagaaagtat tatttcattt ctactagaat tttttgtatt tgtctgccgt
    14161 cgatagaatc acccatacgt tctttttcaa gtttttgact taaatcgata gtatgtgatt
    14221 tgccatcttt catagtaatt gtaatttgac cttgtttaag accatttgaa tacaagccgt
    14281 gttgtttgat tgcacgttct cgtaatctat aatcaagttc ttttaatgag atattttctt
    14341 tataaatttg atacagacta ttttgtggtt cttcattacc tttcttaatt tcaaggattg
    14401 gagagtgtat atagtcgtaa tacactcctc tattagtttt tgttacgcca ccaatcgaat
    14461 gattatcagc ttgtctaccg tttccttctc taacgacaaa cacatctata tctgggttaa
    14521 cttcttcatt atatttatct ttatcagaac caactaaaga tattttatta tattgtccat
    14581 tgtatataaa atcaatgaat ctatctttac cttcaataaa tccatatcca gttacttttt
    14641 tatattcaaa acttggtcca ttataataaa gttttaaatc agcctcactt tgttgtttaa
    14701 cttctgtttt cgcttgtact gattgtatat tcgacgttat tacacctgtt gctaacattc
    14761 ccaatgctaa acttgctttt gctatcgctt taaatttcat aatttttagc cccaatctat
    14821 ttaaatttga tttttcaatt acgtaatatg aacaatctac ctacattata ttgaggcgct
    14881 attccctttc aaattaaacc tcatttaact aaacttgaat cattgttaag gtaattaata
    14941 aaatgctaac tgttaataat caattcttat ccgtcaagac tcttttttat acaattcaca
    15001 ctttaaacca ttttaatttt aaaaactgta aaactttcag acacaaaaat gacatcatat
    15061 cgtcgcagta ccatgtcaaa attttcaaag atcatggtga cacgcatcaa ctaaaacgcg
    15121 tcattacggt atttttaaca ttaataacat atgcgtattt actatacacg gcgacaataa
    15181 atacagattg caatttttta taaaatcaaa tgttttgaaa atcatcgaat aagtattatg
    15241 ttatgtatat aaagattagg agatgaagtg aatgaatatt atgttaacag gtgctacagg
    15301 tcatttaggc acacatatta caaatcaagc cattgcaaat cacatagatc attttcacat
    15361 tggtgttaga aatgttgaga aagttccaga agattggcgc ggaaaagttt ctgttcgaca
    15421 attagattat tttaatcaag aaagcatggt aaaagcattt aaaggcatcg acaccgttgt
    15481 gtttattcca agtattatcc atccatcatt taaacgtatt cctgaagtgg aaaatttagt
    15541 atatgccgca aagcaaagtg gcgttgctca tatcattttc ataggttatt acgcagatca
    15601 gcataataat ccattccata tgagccctta tttcggatat gcatcacgtt tattggcaac
    15661 aagtggcatt gattatacgt atgtaagaat ggcgatgtac atggatccac ttaaaccata
    15721 tttaccagaa ttgatgaata tgcataaact gatttatcca gctggtgatg gtcgtattaa
    15781 ttatattact agaaatgata ttgctagagg tgtcattgct attattaaaa atccagatac
    15841 ttggggcaaa cgttatttat tatcaggcta cagttatgat atgaaggaac ttgctgcaat
    15901 tttatctgag gcatcaggca cagaaattaa atatgaaccc gtttcattag agacatttgc
    15961 agaaatgtac gatgaaccta aaggctttgg tgcattattg gcatcaatgt acgacgcagg
    16021 agcaagagga ctattagacc aagaatccaa tgaattccaa caattagtca atgatcaacc
    16081 acagacactg caatcatttt tacaagaaaa tatttaatac taaaggagcg ttacagtgaa
    16141 tatcatctcg acaattttaa tcatatttgt ggcattagaa tttttctata ttatgtacct
    16201 tgaaacgatt gctacaactt ccagaaagac cagcgagaca tttaatataa gcgtcgataa
    16261 attgaaagac aaaaatatta acgtactttt gaagaatcaa ggcgtatata atggtttaat
    16321 cggagttttg ctaatatacg gtttatttat cagcagtaat ccaaaagaaa tatgcgcagc
    16381 tattttagtg tatatcattg gcgttgctat ttatggtggc ctttcaagca atattagtat
    16441 ctttttcaaa caaggcacat tgccagtatt ggcacttata tcaatgcttt ggtaagtatt
    16501 ggtgttttgg ggtttggaga tgtagtcgga ggtttggagg atttgagcga tttgggtatg
    16561 gactttagac tcagagtatt tcaacctaat tatttcaagc agaggtgaca gtagcgttgc
    16621 ctctgtttcc ttatagaaaa attatttaat gaagaaaatt catatctgtt ttaattttta
    16681 gttgataaat actccatata ttagaatggc tactttatct attgcatcaa tcctttaaaa
    16741 caaaaaaccc atgatttcga aattcccgta tggtggggtt cctactctca tgggtcagtt
    16801 aactaaatta tcactatcag tttataattt caatattatt aacaatatat gtagtcgcaa
    16861 aaggaatgag gacatgagga ctttggtgtt gattggcatt acataacgct tccaaacaaa
    16921 ttattcggta acaataagaa actatttaca caaattatta tacatagtaa aattatttta
    16981 taaactttaa atcaaattgt acaaggagtg aatatcatgg taccagaaga aaaaggttct
    17041 attactttgt caaaagatgc agctaccata tttgcaactg caaaattcaa accatttcag
    17101 aacagaatta aaaataaccc acaaaaaaca aatccatttc ttaaattaca tgaaaacaaa
    17161 aaatcttaat cactttattt atagcatttc tgatctcaga aatttaataa aggacaacgc
    17221 cctctttcat aactatataa gtcaagaaac caagaaattc caaacatctt taaacccttc
    17281 aatagcacat ttcctaaaac ataaagcaat aacaatgaac gatcaaaatt cctaacgtac
    17341 ttatgatgtg tcagtgaatc gtctacactt aattgaacaa attctatgag aatagatatt
    17401 gttaatttaa gaaagtaggc tattttatta cgacaagaga aagaagatca tttagttcag
    17461 agtttaagtt acaaaaggtt agattatatg aaaatggtaa gcctaaaaat gaaattatac
    17521 gcgagtatga tttaacaacc tcaactttct caaatccgat aaaacaacac caaaatactg
    17581 ggtccttcaa tcatcaagat aatttaaaaa gtgatgaaaa agagttaata aaattacgca
    17641 aagaagttca acatttaaaa atggaaaatg atgttttaaa gcaaatattg ctaatcacga
    17701 gacgaaatag aaatcattta acagaatgcg tatcaatatt caatatccat taatgtgcaa
    17761 agtcttagaa gtacttatta tgaatcaata aaaaggaata ataagagcaa aaaacatgct
    17821 gaattggata atcgttcact cccttaacaa atatatgtac ttttgaataa cttgtaatgt
    17881 aaatctactt gactgatata aaccttcatc cactttatga acaattttat ttactaaagt
    17941 tttttatatt tacgataatc ttttacgttt atcttcttat tcaacggtat ctacaaaaac
    18001 actctatacg tttcaattgt atgattgaat gtattatagt gtgcgaaaac tatgcaacac
    18061 tgctttaaac tgatctgaac gcatcatgta tctgctttaa atcatttact tcatcataat
    18121 aaaatacaat tacatatata taatattttt ataattgcag tgatttttga ttaattcgcc
    18181 atgattattt tcacagactt taatatcacc atacacagca acaccactga aacatgtata
    18241 tcgcatttta atttaatttc atccattttt tgtgcctcta atataacaat ttttgtgcgt
    18301 catttaattc ggtcgcaaaa cttatatcag aagatatttt ttctatttct tgaaact
//
LOCUS       DAKSQE010000051        17985 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.051, whole genome shotgun sequence.
ACCESSION   DAKSQE010000051 DAKSQE010000000
VERSION     DAKSQE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17985
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.051"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(147..995)
                     /locus_tag="O0V33_001450"
     CDS             complement(147..995)
                     /locus_tag="O0V33_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCX8976082.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYIDL
                     VLIHQPYNDVYGSWRALEELKETGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
     gene            complement(1065..1481)
                     /locus_tag="O0V33_001451"
     CDS             complement(1065..1481)
                     /locus_tag="O0V33_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCX8976083.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDNDL
                     NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNENIQAQQKQILNKQLEELNENLKTIHD
                     ARDLLQYKIDNYDNHIAKINASDNHDDNVERLWERK"
     gene            1724..1891
                     /locus_tag="O0V33_001452"
     CDS             1724..1891
                     /locus_tag="O0V33_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976084.1"
                     /translation="MLKIWVIDCLYASTFFVDCLTVDSQLYTLTNFEKLNHYLIIIFI
                     AHAQHKKETCQ"
     gene            1888..4314
                     /locus_tag="O0V33_001453"
     CDS             1888..4314
                     /locus_tag="O0V33_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 8 family protein"
                     /protein_id="HCX8976085.1"
                     /translation="MTYRMKKWQKLSTITLLMAGVITLNGGEFRSIDKHQIAVADTNV
                     QTTDYEKLRNIWLDVNYGYDKYDENNPDMKKKFEATENEAEKLLKEMKTESDRKYLWE
                     SSKDLDTKSADMTRTYRNIEKISEAMKHKNTKLKTDENKTKVKDALEWLHKNAYGKEP
                     DKKVADLTSNFKNKTSRNTNLNWWDYEIGTPRALTNTLILLQEDFTDEEKKKYTAPIK
                     TFAPDSDKILSSVGKSEPAKGGNLVDISKVKLLESIIEEDKDMMKKSIDSFNTVFTYA
                     QNSATGKERNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKETPFNDSNQNDT
                     TLKSWIDDGFMPLIYKGEMMDLSRGRAISRENETSHSASATVMKSLLRLSDTMDKSTK
                     AKYKKIVKTSVESDSSYKQTDYLSSYSDISKMKSLMEDSTISTNGLTQQLKIYNDMDR
                     VTYHNKGLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYRDNFWA
                     TADMKRLAGTTTLDNEEPKSTDVKKSSKTFVGGTKFDDQHASIGMDFENQDKTLTAKK
                     SYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANDYKLYKDDTQTTNSDNQETNSLF
                     LESTNSTQNNIGYHFLNESKITVKKESHTGKWSDINKSQKDIQKTDEYYEVTQKHSNT
                     DSKYAYVLYPGLSKDVFKSKASKVTVVKQEDDFHVVKDNESVWAGINYSDSAKTFEIN
                     NTKVEVKAKGMFILTKKDDNTYECSFYNPESTNSVSDIESKISMTGYSIINKNTSTSN
                     ESGVRFELTK"
     gene            complement(4374..5228)
                     /locus_tag="O0V33_001454"
     CDS             complement(4374..5228)
                     /locus_tag="O0V33_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCX8976086.1"
                     /translation="MNKTNFGDYLNKSKSRKIYKIFDQNLKREFSYWQFRKLFKSYTW
                     QSDVHELKVCTQNDGTIEQIWIDKANQFGVAVTWKDNSIIGFVMRPLINYDAIKNETT
                     LKYAVPTTDCYEVFWGGDNELYNYHYLYPHQRYAIDLVIKENHKTYKNLGKVNADYFC
                     FGKDIIAPANGTVEKVVNGVQDNSIGSTKESQFLGNYIVIKHAENEYSLIAHLQQYSI
                     IVNEGQNVKYGDIIGKVGNSGNSTEPHIHFQVMNDKNIEACTSLKIRFINNRELIKGD
                     VVCGLQGE"
     gene            complement(5439..6254)
                     /locus_tag="O0V33_001455"
     CDS             complement(5439..6254)
                     /locus_tag="O0V33_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HCX8976087.1"
                     /translation="MSKRLLLFDFDETYFKHNTNEEDLSHLREMEKLLEKLTNNNEVI
                     TVVLTGSTFQSVMDKMDQVNMTFKPLHIFSDLSSKMFTWNNGEYVESETYKKKVLSEP
                     FLFEDIEDILHHISAQYNVEFIPQRAFEGNETHYNFYFHSTGNHNNDRRILEVLVRYA
                     NDQNYTARFSRSNPLAGDPENAYDIDFTPSNAGKLYATQFLMKKYNIPVKSILGFGDS
                     GNDEAYLSYLEHAYLMSNSRDEALKQKFRLTKYPYYQGITLHVKEFVEGKYDY"
     gene            6549..6974
                     /locus_tag="O0V33_001456"
     CDS             6549..6974
                     /locus_tag="O0V33_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCX8976088.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKEMTMDGY
                     HTVPYTISVDGITALHRTYFVFPENKKVLYQEIDSKVKNQLASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
     gene            complement(7365..8069)
                     /gene="budA"
                     /locus_tag="O0V33_001457"
     CDS             complement(7365..8069)
                     /gene="budA"
                     /locus_tag="O0V33_001457"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCX8976089.1"
                     /translation="MASVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGAVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDEDFIN
                     ANIDYKDIADEIREAE"
     gene            complement(8106..9770)
                     /gene="alsS"
                     /locus_tag="O0V33_001458"
     CDS             complement(8106..9770)
                     /gene="alsS"
                     /locus_tag="O0V33_001458"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCX8976090.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSADE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISEKVDEP
                     FINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            10003..10258
                     /locus_tag="O0V33_001459"
                     /pseudo
     CDS             10003..10258
                     /locus_tag="O0V33_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500937.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10240..10422)
                     /locus_tag="O0V33_001460"
     CDS             complement(10240..10422)
                     /locus_tag="O0V33_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976091.1"
                     /translation="MIDSVFWKKAFIPVYFIVAMLVFLLFRFYIKTNNFSIYLMIIFL
                     MCLGTASIIYNYKNNR"
     gene            complement(11072..11251)
                     /locus_tag="O0V33_001461"
     CDS             complement(11072..11251)
                     /locus_tag="O0V33_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976092.1"
                     /translation="MTEIVEFLSDGKLASRLEIQETMGLQKSYTSELIAELKKLEIIG
                     SEGRGPGTRYYLIRD"
     gene            complement(11500..12936)
                     /locus_tag="O0V33_001462"
     CDS             complement(11500..12936)
                     /locus_tag="O0V33_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HCX8976093.1"
                     /translation="MTSSKYIEYLQTEVEGQLLDRKRASINPKDVAQHISAFANAEGG
                     KLVIGIEDNGEISGFNHSKAKKKEVYIEAPFEHLYRIPKYTYEIIEVKKRDGTMDEIL
                     IFDIEPSYDAIITLKDDSVYLRINDKSRKLTHNQITNLEYDRGSRRFEEELVEYSSIE
                     DVNEELVDEFKQLLNTNVDNEKLLKARGFMREGKLTVAGLLLFSNHINVYLPGARIRF
                     MRYEGTKEESGTRLNIVKDITFDKALPVAIREARAFINTQLREYTFLGKEGRFVTLPE
                     YPEFAWFEGMINSIVHRRYDNQGDHIRIKMFDDRLEISSPGALPASVTLENIKEERYS
                     RNPKLAAALTQYKWVRESNEGVGRIFDEMKQYFLDDPVYSEPNNSSVQLALKNNIIAR
                     KKRETGRVSNIITPELFESLNEQQELIVRHLYNQGELTASKIANIIQRSVPTARKRLK
                     ELEEMNIISTHGSSKNDPKRTYYLLGFE"
     gene            complement(13285..13683)
                     /gene="rpsI"
                     /locus_tag="O0V33_001463"
     CDS             complement(13285..13683)
                     /gene="rpsI"
                     /locus_tag="O0V33_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCX8976094.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            complement(13697..14134)
                     /gene="rplM"
                     /locus_tag="O0V33_001464"
     CDS             complement(13697..14134)
                     /gene="rplM"
                     /locus_tag="O0V33_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCX8976095.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            complement(14383..15177)
                     /gene="truA"
                     /locus_tag="O0V33_001465"
     CDS             complement(14383..15177)
                     /gene="truA"
                     /locus_tag="O0V33_001465"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCX8976096.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRIHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTDLNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQQRDPFQSGLKTFIPEPLDLDKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKL
                     LESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSL"
     gene            complement(15182..15988)
                     /locus_tag="O0V33_001466"
     CDS             complement(15182..15988)
                     /locus_tag="O0V33_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCX8976097.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLT
                     YLWMFALILFFMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGITI
                     ETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMI
                     MSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEE
                     LAVAMEVRGYDANVKRTSYRQLKWQLRDTLSLIMIIPIAIILFVLKYSGV"
     gene            complement(15978..16838)
                     /locus_tag="O0V33_001467"
     CDS             complement(15978..16838)
                     /locus_tag="O0V33_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCX8976098.1"
                     /translation="MIIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKTIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKEKLADWHIGLPEIVQLQYDFEQKHQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            complement(16835..17644)
                     /locus_tag="O0V33_001468"
     CDS             complement(16835..17644)
                     /locus_tag="O0V33_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCX8976099.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFNIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQAITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPHDEMHRRVSEALEQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTATEIFDHAEELTTIGLDLPFPIKINQMLGHQTSFLTYEGLVDQL"
BASE COUNT         5784 a         3209 c         2625 g         6367 t
ORIGIN      
        1 agttattgca taatataaaa attcataata gcttatttgc ttgctccttt cactttttaa
       61 gagtaataaa ttaacctgaa acatagactt taacatccaa gtttcaggtt agcgtaatgt
      121 aaattattta ctttgttata ttatgattaa acatctaatt ctctgcttgt aagtgcttta
      181 atcatctctg gatcagcatg tgagaaaaat tggctattac tttcttctaa tgtcgcaatt
      241 tgttgtttat cttcttctgt aagttcaaaa tcaaatatat ctaagttttg agccatacgt
      301 tctggattca ctgattttgc gagtacaacg atgtcacgtt ctactaacca ccgtaaaata
      361 acttgtgcaa ttgatttgtt atatttatct gcaatggcct gcaacaattg attttggaat
      421 aattgatttt taccttctgc aaatggtgcc caagcttcta ccacaacatt ttcttgttga
      481 agtgctgcaa cttgttcttc ttgttgatgg aatggattaa tttctatttg gttcacttgt
      541 ggttgaattt gattgtgaat acctaaatct acaattcgat caacgccgaa gttagataca
      601 ccgattgctt taattttacc agtttctttt aactcttcta atgcacgcca tgagccatat
      661 acatcattgt aaggctggtg aataagcact agatcaatat agtccaaatt taatcgttgt
      721 aatgatcgtt cgattgattt gatagtatct tcatagttaa cattttcaat ccatacttta
      781 gtcgtaatga atagttcact tctatcaata cctgaagctt caataccttg acctacttca
      841 gtttcattta aataactttg tgctgtatca atatgtctat aacccgcttt aattgcttct
      901 ttaaccgctt ctgctgtttg ttcttgagga atttgaaata ctccgaaacc taatactgga
      961 atctttacat ctttacttat ttcaatatga ttcataaaat gaacctcctt tatcttgtaa
     1021 gtccactata aaccatggag caaactctat agcaagccag aggtttactt tctctcccaa
     1081 agacgttcaa cattgtcatc atgattatca ctagcattga ttttagcaat atgattatca
     1141 taattatcaa ttttatattg tagtaaatct ctcgcatcat gaattgtctt taagttttca
     1201 tttaattctt cgagttgctt atttaaaatt tgcttttgct gtgcttgaat attttcattt
     1261 ttaggaagct gcgctaacct acaaaattcg ataagcgatt caatactgac gcctgcattt
     1321 cgcaaatttt tcactaaata aatccaattt aaatcattat cattgtatat tctatatcca
     1381 ttttcatctc gattaacagg tggaatcaca ccaacctttt cataatatct taaagtgtct
     1441 tgagatatat tcatgagcgc tacgacttct ttagttttca ttgtgactat cctttcagtt
     1501 atgtttggtt gtctaaagta atgttacttt atatattgtc atcttcgttt gaatacttct
     1561 tattttatta ctcaaattta aatttgtctc ttttttaaca ttttacattt catcgttttt
     1621 aattacttta aaaattgtat aacttaaata tttaaaatga tataatcaca aagattgata
     1681 atatttaatt tttaaaaaat attttcgttg caatttttgg gtgatgctga aaatttgggt
     1741 gatcgattgt ttatatgcat caacattctt tgtggattgt ttgacagtag acagtcaatt
     1801 gtatacgtta acgaattttg aaaaattgaa tcattattta atcataatat tcattgcgca
     1861 cgcgcaacac aaaaaggaga catgtcaatg acatatagaa tgaagaaatg gcaaaaattg
     1921 tccaccatta cgttattaat ggctggtgtg attactttga atggtggtga attcagaagt
     1981 attgataaac atcaaatcgc tgtggctgat acgaatgttc aaacgacaga ttatgaaaag
     2041 ttgaggaaca tatggctgga cgttaactat ggttatgata agtatgatga gaataatcca
     2101 gatatgaaga agaagtttga ggctacggag aatgaggcag agaaattact caaggaaatg
     2161 aaaactgaaa gtgataggaa atacttgtgg gaaagctcaa aagatttaga tacgaagtct
     2221 gcggatatga ctcgtaccta tcgtaatatt gagaaaatct cagaagcgat gaaacataaa
     2281 aatactaaat taaaaacaga tgaaaacaag acaaaagtaa aagatgcact tgagtggctg
     2341 cataaaaatg catatggaaa agaaccagat aaaaaagttg ctgatttgac ctcaaacttt
     2401 aaaaataaaa cttctagaaa taccaactta aattggtggg attatgaaat tggaacacct
     2461 agagcattaa caaatacgct tatactctta caagaagatt tcactgatga agaaaagaaa
     2521 aaatatacag ctcctattaa aactttcgcc ccagatagtg acaaaatatt atcttctgta
     2581 ggaaaatctg aacctgctaa aggcggaaat ttagtagaca tttctaaagt aaaactttta
     2641 gaaagtatta tcgaagaaga caaagatatg atgaaaaagt ctatagattc atttaataca
     2701 gtcttcactt acgcgcaaaa ttctgccact ggaaaagaac gtaatggatt ctataaagat
     2761 ggctcttaca ttgatcatca agacgtccca tacactggtg cttatggcgt tgtactatta
     2821 gagggtattt ctcaaatgat gccgatgata aaagaaacac cttttaatga tagtaaccaa
     2881 aatgatacaa ccttaaaatc atggattgac gacggattta tgccactcat ttataaaggt
     2941 gaaatgatgg atttatcaag aggtagagct atcagtcgtg aaaatgaaac gagtcactca
     3001 gcatctgcaa cagtaatgaa atcattgttg agattgagtg ataccatgga taagtctaca
     3061 aaagctaaat ataaaaagat tgtcaagact tcagtagagt cagattcaag ttataaacaa
     3121 accgattatt taagctctta ttcggatata agcaaaatga agtctttaat ggaagacagc
     3181 actatttcta ctaacggttt aacacaacaa cttaaaatat ataatgacat ggatcgtgtc
     3241 acctatcaca ataaaggctt agactttgca tttggtttaa gtatgacgtc gaaaaacgtc
     3301 gcacgttacg aaagtatcaa cggagagaac ttaaaaggtt ggcacactgg tgctggaatg
     3361 tcttatttat acaatagcga tgtgaaacac taccgtgata acttctgggc gacagctgat
     3421 atgaaacgtt tagcaggtac tacaacttta gataatgaag aacctaaaag tacggatgtt
     3481 aaaaagtcta gtaaaacttt tgtaggagga acaaaattcg atgaccaaca tgctagtatc
     3541 ggaatggatt ttgaaaatca ggacaaaact ttaactgcca aaaaatcata tttcatatta
     3601 aacgataaaa ttgtcttctt aggaactggc attaaaagta ctgattcatc aaagaatcca
     3661 gttacaacga ttgaaaatcg caaagcgaat gattataaat tatataaaga tgatacgcaa
     3721 acaaccaatt ccgataatca ggaaaccaat tccctctttt tagagtcaac gaatagcact
     3781 caaaacaata taggttatca ttttttaaac gaatcgaaaa taactgtaaa aaaagaaagt
     3841 catactggta agtggagtga tataaataaa agccaaaagg atatacaaaa aactgatgag
     3901 tattatgaag taactcaaaa gcattctaat acagatagta aatatgcata tgtgttgtat
     3961 ccaggcttat ctaaagatgt ctttaaatcc aaagcaagca aagtaactgt cgttaagcaa
     4021 gaagatgact tccacgttgt gaaagataat gaatcggttt gggctggtat caattatagt
     4081 gatagcgcta aaacttttga aattaataac actaaagtcg aagttaaagc caaaggaatg
     4141 tttattctta caaagaaaga tgataacact tatgaatgta gcttctataa tcccgaatct
     4201 acaaattccg tttcagatat tgaatctaaa atttcaatga ctggatactc tattataaac
     4261 aaaaatacgt cgacttctaa tgaatccggc gtacgctttg aattaactaa ataagcaaga
     4321 aaatctcggc actataccat ttttatagtg tcgagatttt ataagcataa ctatcattca
     4381 ccttgtaatc cgcagaccac atctcctttg ataagttctc gattatttat aaatcgaatt
     4441 tttaaagatg tacatgcttc aatattctta tcattcatta cttgaaaatg tatatgaggt
     4501 tcagtagaat tgccagaatt cccaaccttc ccaatgatgt caccatattt aacattttgc
     4561 ccctcattca caatgattga atattgttgt aaatgagcta ttaagctata ctcattttct
     4621 gcgtgcttga ttacaatata atttcctaaa aactgtgatt ccttcgtact tccaatactg
     4681 ttgtcttgaa ccccattcac tactttttca actgtaccat ttgctggcgc aatgatatct
     4741 tttccaaaac aaaaataatc agcatttact tttcctaggt tcttatatgt tttatgattt
     4801 tctttaataa ctaaatcaat agcatacctt tgatgtgggt ataaatagtg ataattatat
     4861 aattcattat ctccacccca aaaaacctca tagcaatctg ttgtcggcac tgcgtatttt
     4921 aaagtcgttt cattcttaat cgcatcataa ttgattaacg gacgcataac aaatccgata
     4981 atactattat ctttccatgt cactgccact ccaaattgat ttgctttatc tatccatatt
     5041 tgctcaatag taccatcatt ttgagtacac acttttaatt catgtacatc agactgccat
     5101 gtataagatt tgaaaagttt cctaaactgc caataactaa actctcgctt caaattttga
     5161 tcaaaaatct tatatatttt cctgctctta cttttattta aataatcccc aaagtttgtt
     5221 ttattcatat atacttaacc tcttatatta attttggtat caattctttc tccagttcct
     5281 gcgtcatttt tgcgctacct gaatcgtcta cacttaattg gacaaatcaa catataaata
     5341 attttttaaa actaaagtgt agtcgattca ctatattttc taatcatccc ttaaatatta
     5401 atatttgtac ttaaaaagta tacatatcac tcaaccaatc aataatcata cttaccttca
     5461 acaaattctt taacatgcag tgttatccct tgataatatg gatattttgt taatctaaat
     5521 ttctgcttta atgcttcatc tctactattc gacatcaaat aggcatgttc aagatagctt
     5581 aaatatgctt cgtcattgcc gctatctcca aaacctaata ttgattttac tggtatatta
     5641 tatttcttca tcagaaattg agtagcatat agttttccag catttgacgg tgtgaagtca
     5701 atatcatacg cattttcagg atcaccagct aatggattgc ttcgactaaa tctcgctgta
     5761 tagttttggt catttgcgta tctgacaagt acttctagaa tacgcctatc attgttgtga
     5821 ttacctgttg aatgaaaata aaaattataa tgtgtttcat taccttcaaa tgctctttgt
     5881 ggaataaatt cgacattata ttgcgcagaa atatgatgta atatatcctc aatatcttca
     5941 aataaaaatg gttcgctcaa gactttcttt ttatatgttt ctgattcaac atattcgcca
     6001 ttattccaag tgaacatttt agaactcaaa tcagaaaaga tatgtaatgg tttaaacgtc
     6061 atattaactt gatccatttt atccatgaca ctttgaaatg tacttcctgt caatacaaca
     6121 gttatcactt cattattatt agttaatttt tccaacaatt tttccatttc tcttaaatga
     6181 cttaaatctt cttcatttgt attatgttta aaatatgttt catcaaaatc aaacaataat
     6241 agacgctttg acatactcat tcctccataa tattttaatg aatttatcta taacttaaat
     6301 acactataag tctcaattaa aagtataaga tcgactatat gcttttcatg cttattatat
     6361 acaactaatg aatccctata aactaaaaac attgcttacc acttttcaaa atacacgggt
     6421 catatccctc gttaaaaact attactaaaa atcaaaaata taaaaaaatc aaaaagtatg
     6481 ttttaaactt tatgaaacgt gttatataaa tagtgtaata taccacaact ttaaaaggag
     6541 attgagatat gaaactaaaa tcatttgtta ctgccacttt agcattgggc ttattatcaa
     6601 cggtcggagc tgcattacct agtcacgaag catctgcaga tagtaataac ggctataaag
     6661 aaatgacaat ggatggttat cacactgttc cttacacgat ttcagtagat ggtattactg
     6721 cattacatcg aacttacttt gtttttccag aaaataaaaa agtactttat caagaaattg
     6781 acagtaaagt aaaaaatcaa ttagcttctc aacgtggtgt tacaacagaa aaaattaata
     6841 atgcacaaac agcaacgtat acacttactt tgaatgatgg taataaaaaa gtagtgaatc
     6901 taaagaaaaa tgacgacgct aaaaattcaa ttgatccaag tacaatcaaa caaatacaaa
     6961 ttgtagttaa ataataattt gaaaatgaaa atagttatac agggcaaacg ctgaaaaatt
     7021 gcgtttgtct ttttagtatg aaatgaataa tcgaacttgt aatttattat tgattgcttc
     7081 tactgacaat agattagatt ggcctatact aaagtaaatt ctatattaac gtcttccgta
     7141 atttataatc taacataaca gcaaattaca aatagctaga acataaataa atcccctcac
     7201 taccgttaat agtgaaggga tttattttat tacatagacg tatgctctat gacaaccatg
     7261 cttaaccgta ttacacaacc ttttatcatt gctaaatttc caaatcgaac attatgaagt
     7321 caatgcttca atttttataa tccatcatta catttccgca ttcattattc agcttctcta
     7381 atttcgtctg caatatcttt atagtcaata tttgcattta tgaaatcttc atcttgaatt
     7441 ggaaaatgct gttcaaatgt ttctatattt tggatttcta ccttaacatc ttctacttca
     7501 aaatctaaga catgtccacc aaagttacga tcatcatttg caaagtgtac atggaatcct
     7561 gctgacccga taccatggaa tagttctggt gtaaagaacc caacgactgc gcctttgacg
     7621 tacgtttcag tttgttcagg ttgtcttcta gctgagtcaa ttaaacgtgt ataaggtggc
     7681 tcttgtgccg gcatcatacg tacatgcatt tttttaaata agcctgaaat ttttactgca
     7741 gaaaataaat tttcactcaa catcttttcc ttaatttctg caaaaactgc ttcagaagat
     7801 ttatctttcg tttcatagct tgtatctgct acgaatttag ttacagttgc atatggtgtt
     7861 aactcatcac cttttaattc cacaaattct ttatgttcat ttgcatgata ggcttttcca
     7921 tctaaaaaga tgacctcacc atttgaacct gttagtgtag cgatacctaa atcaccatgt
     7981 tgcaataatt catttattga tgcagttcct tttaataaac ctgccattaa tgtgcctaat
     8041 gtaccatgtt gatataaaac actagccata aaatttccca ctcctcatta acatttatct
     8101 ttgttttagt taaatacgtc aggtaacata tttgttgaaa gtttgatatt atctttgtaa
     8161 tttacaggta tatcaattaa tactggacca tctgtttcat agccctcttt aattgccgct
     8221 tctaattctt cttgattagt aactcgtaaa ccttttgcgc caaatgattc agcatatttt
     8281 acgaaatcta caggaccgaa gtcgacacct gatgaacgtt tatatttcat ttcttcttgg
     8341 aattcaacca tgttatattt tccatcgttc caaataagtt gaataatgtt tagattttta
     8401 cgtacggctg tttctaaatc ttgtgatgaa aataaaaagc ctccatcgcc agcaacggat
     8461 acaacttgcg tattagggcg tacaagcgca gctgaaattg cccacggtaa tgctacacca
     8521 agtgtttgca taccgttact aaataataaa tgtcttggat tgtaacttct gaaattacgt
     8581 gccatccaaa tatagtgact tccaacatca actgtcactg ttgtatcatc agttaatacc
     8641 ttttgcatag attcaataat ttcaactgga tgtagaattc cttcttcatg cgtcgcttta
     8701 ataccagttt cttcatcaat atgtgttctt aattgttcta attcgtctaa atgttgttga
     8761 tttataaatg gctcatctac tttttcagaa atcatttcaa tcgttttagc aatattacca
     8821 atcaactctt ttttcggttg catataatta gtaatttcag cttgaacttc gtcaatattg
     8881 ataatttgtg tttctaattc tttattccag ttactagctt cgtattcaat tggatcataa
     8941 ccgattgtaa caactaaatc acttttacgt aataattcat caccaacttg attacggaat
     9001 aagcccacac gaccgaagaa atgattttct aattcacgac taattacacc tgcaccttgg
     9061 aatgtttcta caactggtaa attcgtacgc tctactagct tgcgaatggc attcgtctcg
     9121 tcagcactag aacttctcat accagctaat aaaacaggaa atgatgcatt tttaatcgct
     9181 tcaatgacat cattaatatc ttgttcactc ggtactccta aatttggttt ttggcaaagt
     9241 gatatagctt tagattcaac tggtgaagaa atcacgtctt gcggaatact aataaaactt
     9301 gcgccatttt tccctgaagt agcaatacga attgcatttg tcataacttc tgataatgat
     9361 tcaggatctt gtacttcttc actgtatttt gatgagtatt ttaatagcgc agcattatca
     9421 atactttgat gcgttaatcg taataaatca ttacgtttca cttggccgcc taacgccaag
     9481 acaggatccc cttcagatgt agctgttaat agtccagtcg ttaaattact tacaccagga
     9541 ccacttgtaa caagtactac acccggtttg cctgttaatc ttccaatacc ttgcgccatc
     9601 attgcggcgt tttgttcatg acgagtgaca ataagttcag gaccatcatc aattaaagca
     9661 ttaaatagat agtctatctt tgcacccgga ataccaaaaa catattctac cccattattt
     9721 ttcaaagtat caataaccat atcggctgca gtgtactttt tatcagtcat ttatattcat
     9781 ttcccttcaa atgtgatgtg tagatgtgta ttacatgtaa ttacattgtg atttgtaatc
     9841 taatgatagt tatgtaaaat taatgatatt tatcaaaact ttacatatac aattagattt
     9901 gtgatacata aaaattgcct tcagtacatt atatatcctt tgtaagatga ataaaaatcg
     9961 ttatttgtaa tactaagtat tccaaaaatg catgagtaga ttttgaaggg agtgattata
    10021 agggatgttg gattgattgg tgtggaggat tgttagcata tttgagaatg aggtctttag
    10081 tcaatgacgg tttgaaattg tcgttttatg gcgggctgaa aatgctgttt tatgacggtt
    10141 tgaaaatgct gtttttggcg atttaaaatt gtcatttcga taaaaaaagc cacagcaaga
    10201 cttagatcta actgtgactt atacatttca tacttatatt tatcgattgt ttttatagtt
    10261 ataaatgata gatgcagttc ctaaacacat taagaagata atcattaagt atatagaaaa
    10321 gttatttgtt ttaatataaa acctaaaaag tagaaacacc agcattgcaa caataaaata
    10381 aacaggaata aaagctttct tccaaaatac agaatctatc aattaagtca ctccttattt
    10441 tgataacact cttaaatcat ggttcttgag ctatcttccg attttcatcc gatagccctt
    10501 tcccttgaac cactatgatt aaaacattat tgtccgtcct ccgcaagcta aatgacaatt
    10561 taataatgta cacttttgat tgtgtaagca tgttcaaatt atacgtgtaa atcaataatt
    10621 catagttgaa tttattctat tccttagaca ttaaataaag gaaaattcat tatactctac
    10681 acattccata ttccccacac aaacgtaaaa aaacacctcg acataatatc gaggtgtttt
    10741 gtatatatcg tccgacaatt aacgttttga gaattgaggt gaacgacgag ctgctttaag
    10801 acttggtttt ttacgttcaa aaaactttag ggtttcgtac aattgtatag actgtcgtaa
    10861 gccttgatac taaaggattc ctaatttttt cgttcacata gaattgcatc aaatatcacc
    10921 taggggtaac taaagggtaa ctaaataaac tgctgtccgg tggtatatac tctcgtatca
    10981 tccggttatc gtccggtcgt aaatatattg atttatcaat gtttttatgt atatcaatag
    11041 gttatcgtcc ggttatcact tgatgaattt attagtctct aattaaataa tatctagttc
    11101 ccggacctct accttcagat ccgataatct ctaatttctt tagttcagca attaactcag
    11161 atgtataact tttttgaagt cccattgttt cttgaatttc tagcctactt gctaacttac
    11221 catctgacag aaattctaca atctctgtca acttctgatt agtaggtatt ttatttaact
    11281 tgtaattctt attaaatagt actactcgaa aatccgcctc tttactcgaa agctcacaaa
    11341 actcttgata tcactggttt ctcatgattt tttaatgttt ttattttgag ttactcgaaa
    11401 attttcctct ttactcgaaa gctcacaaaa cccttgatat cattggtttc tcatgatttt
    11461 ttaatatttt tcttttgagt tactcgaaaa tttttctctt tactcgaatc cgagtaaata
    11521 atatgttctc tttggatcat tcttacttga tccatgtgtc gatataatat tcatttcctc
    11581 taattctttc aatctttttc tagcagtagg aacagacctt tgaataatat tagcaatttt
    11641 agatgctgtt agttcgcctt gattatataa atgtctcaca attaattctt gttgctcatt
    11701 taaactttca aataattcag gtgttattat attactcact ctccctgttt ctcttttttt
    11761 ccttgctatt atgttattct tcaaagctag ttgtactgaa ctattattag gctctgagta
    11821 tacaggatca tctaaaaaat attgcttcat ttcgtcaaat atacgtccca cgccctcatt
    11881 tgattctctc acccatttat attgagttaa tgctgcagct aattttggat tccttgaata
    11941 tctttcttct ttaatattct ctaacgtaac agaagcaggc agtgcacccg ggctactaat
    12001 ttctaatctg tcgtcaaaca tttttattct tatatgatcg ccttgattat catatctgcg
    12061 atgtacaata gaatttatca tcccctcaaa ccaagcaaat tctggatatt ctggcaaagt
    12121 tacaaatctt ccctctttac ctaaaaaagt atattcacgt aattgtgtat tgataaaggc
    12181 tctcgcttct cttattgcta ctggcaaagc tttatcaaaa gtaatatctt ttacaatgtt
    12241 aagtcttgtc ccagattctt cttttgttcc ttcatacctc ataaatcgaa ttctagcgcc
    12301 tggtaaataa acattgatgt gattactaaa caataacaaa cctgcaactg tcaatttacc
    12361 ttcacgcata aatcctctag cttttaataa tttttcatta tctacgtttg tattaagtaa
    12421 ttgcttaaat tcatcaacta gttcttcatt cacatcttca atactagaat actctactaa
    12481 ttcttcttcg aatcttcgtg aaccacggtc gtattctaga tttgtaattt gattgtgtgt
    12541 cagttttcta cttttatcgt taatccttaa atatactgaa tcatctttta aggtgattat
    12601 ggcatcataa ctgggttcaa tgtcgaaaat aagaatttca tccatagttc catcgcgttt
    12661 cttcacttct attatttcat atgtatattt aggtattcta tataaatgtt cgaaaggtgc
    12721 ttcaatataa acctctttct tttttgcttt tgaatgatta aatcccgaaa tttctccatt
    12781 atcttcaata ccaattacca gtttgccacc ttcagcattt gcaaacgcac taatgtgttg
    12841 agcaacatct ttaggattta tagatgccct ctttcgatct agaagctgtc cttcaacctc
    12901 agtttgcaaa tattcaatat acttactact agtcatatat aaacctcgca atctcgcatc
    12961 ttattgactt tattataaca ttcaatacta tatctatcga atatcattac aacctctatc
    13021 tgcttgtcgt acgaatccca aagaaaaacc aggtcatttc atatggtttc ctaaagtttc
    13081 atatcattga attttaagca gaattgaaac aaattagttc ctctaggggt aactaaatag
    13141 tatctaaatg actctataaa aacacaaaaa acactctttg gaataatcca aagagtgctg
    13201 taaacgtttc ttcagttgaa cacaaattgc tgacgcaatt tgcgaactcc atctgtaagc
    13261 gaacaagttc gcaacagctg cagcttaacg ttttgagaat tgaggtgaac gacgagctgc
    13321 tttaagacct ggttttttac gttctttcat acgtgggtca cgagtaagta atccagcgcg
    13381 ttttaaagaa cctctgtatt caggatctgc ttctaataat gcacgagcga ttccgtgacg
    13441 gatagcttga gcttgtccag tgaaaccacc accatgtacg ttaactaaaa catcatagtt
    13501 acctttagtt tcagttacat caaatggttg gtttaagtct aaaattaatg attcgaatgg
    13561 taagtattcg cgtacgtcac ggttattaac tgtgatgtta ccttcacctg gtactaaacg
    13621 tacacgtgct actgagtttt tacgacggcc tgtgcctcta tattcaactt gtgccaaagt
    13681 catttcctcc ttctaattaa ccacgtaatt cgtagttttc tggttgttgt gcagcgtgtg
    13741 gatgttcagc gccaccatat acaaataatt ttttaccttg tttttcgcct aaacgagtgc
    13801 ttggtaacat acctttaatt gaattttcaa ttaaacgttc tgggttagtt cttcttaatt
    13861 caccagcagt gattgatttg ataccacctg ggtgatttga gtgacggtag taaactttgt
    13921 cagtttcttt gttaccagta aattcgattt ttgatgcatt aataacgatt acataatcac
    13981 cagtatcaac gtgtggtgtg taagttactt tatttttacc gcgtaagata gatgctactt
    14041 ctgatgataa acgacctaat gtttggcctt cagcatcgat aacataccat ttgcgctcaa
    14101 tgtttgattc atttgccata aatgtttgac gcataataat tgtcctccta aaaaataaat
    14161 gcttctattc aagctataaa cagtttcata acctaataga tgatttcatt tgtttcattt
    14221 ttaaaacgtt tcatttcatt tattttgtta actgttgttc gtttctgctt atatatcttg
    14281 taacacaata agtttccggg gcttatcgtg gggtggatat aaaacaatac cgttaataat
    14341 cttataattt cacaagcttt ttgtcaatgg caatcagtca ttttataatg attttttacg
    14401 atgtattttg atatcgttac caaagtcttt taataactca ttttcatcta aataaatttt
    14461 ctctaaatat aatccttcag ccggtgcagt gaaaggtaca ttcttacgat tctttgattc
    14521 aagaagtttt ggtacatctg atacttcatg tcgccctttt ccgacttcta ttaaaaatgc
    14581 aactaagaca cgcaccatgt tatataaaaa tccagatcca gtgacaatat aatcaaagcc
    14641 atcatctgtc tttacaattt cactttggta taatgttcta accttgcttt ctacttcagt
    14701 tttttgcgaa caaaaacccg taaaatcatg tgtacctatg aattgttgtg ccgctctgtt
    14761 cattttgtct aaatccaatg gttcaggaat aaatgttttc aaaccacttt gaaatggatc
    14821 gcgttgttgt gcttgatata ctttatatcg atagcgttta ccgacacaat cgtaacgaca
    14881 atgaaaatcg tcatcgactg taactacatt gttgacataa atatcatcag gtaacgttcg
    14941 gttcattgca tattgccatt gtgacatagg tatattcaaa tctgtgtcga aatgaaagta
    15001 ctgctgtatc gcatgtacac ctctatcagt cctacttgaa ggatggattc tcacatgtct
    15061 tttatgaatg cgttgtaata gcttttcaaa ttgttgctgt accgttcgac cattttgttg
    15121 aatttgaaaa cctagaaaat tatttccttg atacgcaatt tctactaata tacgcatgaa
    15181 tttacactcc tgaatatttt aatacgaata aaataattgc aattgggata atcatgatta
    15241 aagataacgt atctctcaat tgccatttaa gctgtctgta gctcgttctc ttaacattgg
    15301 catcataacc cctaacttcc attgcgaccg ctaattcttc ggcgcgttgg aaagctgaga
    15361 tgaatagtgg cactagtaat ggaataaatg atttaatacg tgttgcaatg ttccctgaac
    15421 ttatttcaga accacgcgac ttttgcgcca aaatgatttt atctaactca tccattaacg
    15481 tcgggatgaa tcgtaacgca atggacatta tcatacttaa ttgatgaact ggtaatttaa
    15541 acatctttag tggcgcaagt aatctttcaa acgcatctgt taaatcaatt ggacttgtag
    15601 atagtgtcat tattgttgca atcatcacaa tcccaattaa acgcagtgat atatatagcc
    15661 cttctaaaat accattagtt tcaatcgtga tgccatgcca ttcaactaat acatatccac
    15721 ctttagttaa aaatatatgc atcattaatg tgaagattaa aaagaaaaat attggcgtta
    15781 aacctttgat taggaaccat aattgaattt ttgctaatct cataaagaat aagataagtg
    15841 caaacatcca aagatatgtt aatggtgaat gacagaaaaa tattaatata ataaaaagaa
    15901 aaacaaacat taactttgct cttggatcaa gatgatgaac aaaagaatta attggtaaat
    15961 atcgccctat aatcaattta tttttcatgt tgccactcct tatacaagct tacaaatgct
    16021 tcttcagtta aggcaatatc ttttaatttt gtttgatgtt tttgttcaaa gtcatattgt
    16081 aactgaacaa tttcaggcaa accaatatgc caatccgcca atttttcttt atctttgaag
    16141 agctcttttg gtgatgtttg cgacacgata ctaccttctt tcattacgat gacttcatct
    16201 gcataacgtg cgacttcatt catatcatgt gaaattagga taattgtctt attttcatct
    16261 gtttgtagtg acttgagtaa tcgcattact tgtcgtttac tttgtggatc aagtcctgct
    16321 gtaggttcat caaccacgat aatatcagga ttcattgcca atatcgatac aatcgctatt
    16381 ttacgcattt gtccacctga catttgaaat ggtgattgtg acattacatc tcttgaaaag
    16441 cctaaatcca tcaacagacg atgggcatag tttttggctt catctaaatt cattttaaag
    16501 tttttaggtc caaatatcat ttcacgctct actgtgtcct caaataattg agattcggga
    16561 aattgaaata ccattccaat tctttttctt acaggtctaa tatatttatc tttggtctta
    16621 tgtgtaatag taatatcatc aactgtaact gtcccagtag ttggctttaa cagtgcatta
    16681 atattttgta tcaatgtcga tttaccacta cccgtttgtc caacgatggc ataatattta
    16741 ccttgttcaa attctgtatt aacatcatga atagcttgat gctggtatgg tgtccctttt
    16801 tgataggtat aacttacatt gtcaaagcgt ataatcatag ttgatccacc agcccttcat
    16861 aagttaagaa tgatgtttgg tgtcccagca tttgatttat tttgattggg aatggcaaat
    16921 ctagacctat tgttgttaac tcttctgcat ggtcgaaaat ttcagtcgct gttccttctt
    16981 tatagacagt ccctttattc ataacgataa catgatctgc ttccatcgcc tcagataaat
    17041 catgcgtaat agaaatgatt gtaatattat gttctgattt aacttttctc actaaatcca
    17101 ataaattttg acgtgcatca ggatctaaca tagaagtcgc ctcatctaat ataatgacag
    17161 aggggttaag tgctaataca cttgctatag ccacacgctg cttctgtccc cccgataatg
    17221 cattaggttc ataatctgca cgttctaaca tatctacttg ttcaagtgct tcgctgactc
    17281 ttctgtgcat ttcgtcatgt ggaaccgcat gattttcgag tccaaatgcc acatcgtatt
    17341 ttacaattga accaacaaat tgattatccg gattctgaaa tacaattcct atgtcttttc
    17401 ttaacttttc aaaattatca tctgttatag cttgattatt ataaaaaatt tctccagatt
    17461 taactttctc tatgccaatc attaacttgg caattgtaga tttcccagaa ccgttatgac
    17521 caacaataga tgtccactga cctttaggta tattaaaaga aacatctttc aatgtgaagg
    17581 atgcatcact ttgatattga aatgaaacat ttttaaatac aataactgaa ttcttatcct
    17641 ccacttgtct ctctccttta cgattcgtgt atctatcata ttttacaata tttataaatc
    17701 gctgtatatg acattgactg ggtttgctat atattactag tattttctga ctcatttcta
    17761 gtctttaaag tgttgtttaa caactaatga taaggacatt tattcctctc taacaattat
    17821 gtataaacgt taataaaata aatgatttac tagtgttggc cgcgtttgat tcaacgataa
    17881 tactttcact tcattcagtt ctagtgaaat tgatcaaact agcttgatca tatttttaga
    17941 ttcgcactca aaaaagtaga tataaagaaa tcggacttaa aaaca
//
LOCUS       DAKSQE010000052        17787 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.052, whole genome shotgun sequence.
ACCESSION   DAKSQE010000052 DAKSQE010000000
VERSION     DAKSQE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17787
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.052"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            131..2227
                     /gene="pnp"
                     /locus_tag="O0V33_001469"
     CDS             131..2227
                     /gene="pnp"
                     /locus_tag="O0V33_001469"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCX8976100.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPTERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            2463..4136
                     /locus_tag="O0V33_001470"
     CDS             2463..4136
                     /locus_tag="O0V33_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCX8976101.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKEKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            4393..6762
                     /locus_tag="O0V33_001471"
     CDS             4393..6762
                     /locus_tag="O0V33_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCX8976102.1"
                     /translation="MAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHRDVAKVALENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFD
                     AENSSNNIVNHHQADQQEQLIEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAK
                     QKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLH
                     NDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGR
                     DISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVE
                     LNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQN
                     QELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSV
                     DVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQG
                     AFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKA
                     STSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            6767..7480
                     /locus_tag="O0V33_001472"
     CDS             6767..7480
                     /locus_tag="O0V33_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCX8976103.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNESDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            7511..8776
                     /locus_tag="O0V33_001473"
     CDS             7511..8776
                     /locus_tag="O0V33_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCX8976104.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTITARSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKETFINDI
                     QKVSREDIVSVAEKAFLDTIYVLTKGGDK"
     gene            8776..10062
                     /locus_tag="O0V33_001474"
     CDS             8776..10062
                     /locus_tag="O0V33_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCX8976105.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEENAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFNTLR
                     AMYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVDPEAICRIVK
                     QHEDARNKVNQPKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            10062..10766
                     /locus_tag="O0V33_001475"
     CDS             10062..10766
                     /locus_tag="O0V33_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCX8976106.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTNKFN
                     DDKVHFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSDNGRIIVISSIWGETGASMETIYSTMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNA
                     RSVTGTIQKVNGAWYI"
     gene            10871..11698
                     /locus_tag="O0V33_001476"
     CDS             10871..11698
                     /locus_tag="O0V33_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HCX8976107.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            11717..12109
                     /locus_tag="O0V33_001477"
     CDS             11717..12109
                     /locus_tag="O0V33_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCX8976108.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVIMGITVLITLLLWIMLVLIF"
     gene            12143..12721
                     /gene="pgsA"
                     /locus_tag="O0V33_001478"
     CDS             12143..12721
                     /gene="pgsA"
                     /locus_tag="O0V33_001478"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCX8976109.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIEYFYKGRDVFKQK"
     gene            12946..14097
                     /locus_tag="O0V33_001479"
     CDS             12946..14097
                     /locus_tag="O0V33_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HCX8976110.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLANHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETVLIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKINEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDNNLEFKTEQFKMSESRNLLKNRSQNYALIRLLNWLRTTN"
     gene            14262..15305
                     /gene="recA"
                     /locus_tag="O0V33_001480"
     CDS             14262..15305
                     /gene="recA"
                     /locus_tag="O0V33_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCX8976111.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
     gene            15659..17218
                     /gene="rny"
                     /locus_tag="O0V33_001481"
     CDS             15659..17218
                     /gene="rny"
                     /locus_tag="O0V33_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCX8976112.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
     gene            complement(17514..17729)
                     /locus_tag="O0V33_001482"
     CDS             complement(17514..17729)
                     /locus_tag="O0V33_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976113.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIATNCYDTAEVIKELKKTDLQLK"
BASE COUNT         6505 a         2383 c         3408 g         5491 t
ORIGIN      
        1 aagtctattt tgtaaataat agatggatat cccgctctct ttttttccaa ttcaatattt
       61 tataactaat attaaaatac gataataaat gatatgatat aactattaga ttcaagagag
      121 gagatttata atgtctcaag aaaagaaagt ttttaaaact gaatgggcag gaagatcttt
      181 aacgattgaa acagggcaat tagctaaaca agcaaatggc gctgtattgg ttcgttatgg
      241 agatacagtc gtgttatcga cggcaactgc atcaaaagaa cctcgtgatg gagatttctt
      301 cccattaaca gtgaactatg aagaaaaaat gtacgctgcg ggtaaaattc ctggtggatt
      361 taaaaagaga gaaggacgtc ctggtgacga tgcaacatta actgcgcgat taattgatag
      421 accaattaga cctttattcc ctaaaggata taagcatgat gttcaaatta tgaacatggt
      481 attaagtgca gatcctgatt gttcaccaca aatggctgca atgattggtt catctatggc
      541 gcttagtgtg tcggatattc cattccaagg gccaatcgcc ggtgtaaatg tgggttatat
      601 tgatggtaaa tatatcatta acccaacagt agaagaaaaa gaagtttctc gtttagacct
      661 tgaagtagct ggtcataaag atgcagtaaa catggtagaa gcaggcgcta gtgagattac
      721 tgaacaagaa atgttagagg cgattttctt tggtcatgaa gagattcaac gtttagttga
      781 tttccaacaa caaatcgtcg accacattca acctgttaaa caagaattta ttccaacaga
      841 gcgtgatgaa gcgctagttg aacgtgtaaa atctttaacc gaagaaaaag gacttaaaga
      901 aacagtttta acatttgata aacaacaacg agatgaaaat cttgataact taaaagaaga
      961 aatcgtcaat gaatttatcg atgaagaaga tccagagaat gaattactta ttaaagaagt
     1021 ttatgcaatt ttaaatgaat tagtgaaaga agaagttcga cgtttaattg cagatgaaaa
     1081 aattagacca gacggccgta aacctgatga aatccgtcca ttagattctg aagttggtat
     1141 tttacctaga acgcatggtt caggtctatt tacacgtggt caaactcaag cactttcagt
     1201 tttaacatta ggtgctttag gtgattatca attaattgat ggtttaggac ctgaagaaga
     1261 aaaaagattc atgcatcatt ataacttccc gaatttttca gtaggtgaaa ctggtccagt
     1321 acgtgcgcca ggtcgtcgtg aaattggaca tggtgcgtta ggtgaaagag cattaaaata
     1381 tattattcct gatactgctg atttcccata tacaattcgt attgtaagtg aggtacttga
     1441 atcaaacggt tcatcatctc aagcgtcaat ttgtggatca acattagctt taatggatgc
     1501 gggcgtaccg attaaagcac cagttgctgg tattgctatg ggccttgtta cacgtgaaga
     1561 tagctatacg attttaacag atatccaagg tatggaagat gcattaggtg atatggactt
     1621 taaagtcgct ggtactaaag aaggtattac agcaatccaa atggatatta aaattgacgg
     1681 tttaacgcgt gaaattatcg aagaggctct agaacaagcg agacgtggtc gtttagaaat
     1741 aatgaatcat atgttacaaa caattgatca accacgtact gaattaagtg cttacgcgcc
     1801 aaaagttgta actatgacaa ttaaaccaga taagattaga gatgttatcg gacctggtgg
     1861 taaaaaaatt aacgaaatta ttgatgaaac aggcgttaaa ttagatatcg aacaagatgg
     1921 tactatcttt attggtgccg ttgatcaagc tatgataaat cgtgctcgtg aaatcattga
     1981 ggaaattaca cgtgaagcgg aagtaggtca aacttatcaa gccactgtta aacgtattga
     2041 aaaatacggt gcgtttgtag gcctattccc gggtaaagat gcgttgcttc acatttcaca
     2101 aatttcaaaa aatagaattg aaaaagtgga agatgtatta aaaattggtg acacaattga
     2161 agttaaaatt actgaaattg ataaacaagg tcgagtaaat gcatcacaca gagcattaga
     2221 agaataatat ttaaagtcat atgacgacaa tgtatcgtca tgtgattttt ttatgccatt
     2281 ttttacgaag tgacccattt tgaatttgtt gtattgaaca ttttaaaacg ctttattatt
     2341 ttgtgtacaa ctgttaatta tcctgtatgt atagtgatta atagtgtaca tcaagtattt
     2401 tttaacttat aatgaatagt gagtttatat atggacgggt aacaaattta ggaggtaaga
     2461 ttttgagttt aataaagaaa aagaataaag atattcgcat tataccatta ggcggtgttg
     2521 gcgaaattgc taaaaatatg tatatcgttg aagtagacga tgaaatgttt atgttagatg
     2581 ctggacttat gtttccagaa gacgaaatgc taggtattga tattgttata ccagacattt
     2641 catacgtact tgaaaataaa gaaaaattga agggtatatt ccttacacac ggacatgagc
     2701 acgcgattgg tgcagtgagt tatgttttag aacaattaga tgcaccagta tatggatcta
     2761 aattgacaat agcgttaatt aaagaaaata tgaaagcccg taatattgat aaaaaagttc
     2821 gctactacac agttaataat gattcaatta tgagattcaa aaacgtgaat attagtttct
     2881 ttaatacgac acacagtatt cctgatagtt taggtgtttg tattcacact tcatatggtg
     2941 ccattgtgta tacaggtgaa tttaagtttg accaaagttt acatggacat tatgcaccag
     3001 atattaaacg tatggcagag attggtgaag aaggcgtatt tgtcttaatc agtgattcta
     3061 ctgaggcaga gaaacctgga tataatactc cggaaaatgt gattgaacat catatgtatg
     3121 atgcttttgc aaaagtgcga ggtcgcttga ttgtttcatg ttatgcttcg aactttatac
     3181 gtattcagca agttttaaat attgctagca agctaaatcg taaagtgtca tttttaggaa
     3241 gatcacttga aagttcattt aatattgctc gtaaaatggg gtatttcgac attcctaaag
     3301 atttgctaat tcctataaca gaagttgata attatcctaa aaatgaagtg ataattatag
     3361 ctactggtat gcaaggagaa cctgtagaag ccttaagtca aatggcgcaa cataagcata
     3421 aaattatgaa tatcgaagaa ggcgattctg tatttttagc aattacggct tctgctaata
     3481 tggaagttat cattgcgaat acattaaatg agcttgtacg tgctggcgca catattattc
     3541 caaataacaa gaagattcat gcttcaagtc atggttgcat ggaagaatta aaaatgatga
     3601 ttaatattat gaaacctgaa tactttattc ctgtacaagg tgaatttaaa atgcagatag
     3661 cacatgcgaa gctagcagct gaagcaggtg ttgcaccaga aaagattttc cttgtggaaa
     3721 aaggagatgt cattaattac aacggtaaag atatgatatt aaatgaaaag gtaaattcag
     3781 gaaatatttt aatagatggt attggtattg gggatgtagg aaatatcgtg ttgagagacc
     3841 gtcatctttt agcagaagat ggtatcttta ttgctgtagt aacgttagat cctaaaaata
     3901 gacgtatagc tgcgggacct gaaattcaat ctcgtgggtt tgtatatgta cgtgaaagtg
     3961 aagacttatt acgtgaagca gaagagaaag tacgtgaaat agtagaagct ggtttacaag
     4021 aaaaacgcat agaatggtct gaaattaagc aaaatatgcg tgatcaaatt agtaaactat
     4081 tattcgaaag tacaaaacgt cgtcctatga ttattccagt aatttctgaa atttaatcaa
     4141 aaagtcatta acataaaaga ggtcagaaca agtcaccgaa atataatggt tgtcatggac
     4201 aatttactta tattttatga tggtcaattg aaaggggtaa cgattaatct gttatcttaa
     4261 gtaaattgat acatagatga tattgttcta acctctttca tcgtctgttt ggactacata
     4321 ttctaaacat caaataggaa attatataga ttaacgtcgt tttaactaaa gcaacataag
     4381 gaggtgcgtc aattggcaca agcaaaaaag aaatcgacag ctaagaaaaa aacaacatca
     4441 aaaaaaagaa caaattcgag gaaaaagaag aatgataatc cgatacgtta tgtcatagct
     4501 attttagtag ttgtattaat ggtgttgggt gttttccaat taggaataat aggtcgtcta
     4561 attgacagct tctttaatta tttatttggg tacagtagat atttaacata tattttagta
     4621 ctcttagcaa ctggttttat tacatactct aaacgtattc ctaaaactag acgaacggct
     4681 ggttcgattg tattgcaaat tgcattgcta tttgtatcac agttagtttt tcattttaat
     4741 agtggtatca aagctgaaag agaacctgta ctttcttatg tgtatcagtc ataccaacac
     4801 agtcattttc caaattttgg tggcggtgta ttaggctttt atttattaga gttaagcgta
     4861 cctttaattt cattatttgg tgtatgtatt attactattt tattattatg ctcaagtgtt
     4921 attttattaa caaaccatca acatcgtgat gttgcaaaag ttgcactgga aaatataaaa
     4981 gcttggtttg gttcatttaa tgaaaaaatg tcggaaagaa accaagaaaa acaattgaag
     5041 cgtgaagaaa aagcgagact taaagaagaa caaaaggcac gtcaaaatga acagccacaa
     5101 ataaaagatg tgagtgattt tacggaagtg cctcaagaaa gagatattcc aatttatggg
     5161 catactgaaa atgaaagtaa aagccagagt caaccaagtc gaaaaaaacg agtgtttgat
     5221 gcagagaata gttcgaataa catcgtaaat catcatcaag cagatcagca agaacaatta
     5281 atagaacaaa ctcataacag tgttgaaagt gaaaacacta ttgaagaagc tggtgaagtt
     5341 acgaatgtgt cgtatgttgt tccaccgtta actttactta atcaacctgc aaaacaaaaa
     5401 gcaacatcta aagctgaagt gcaacgtaaa ggacaagtac tagagaatac attaaaagat
     5461 tttggggtaa atgcaaaagt gacacaaatt aaaattggtc ctgcagtaac tcaatatgaa
     5521 attcaaccag ctcaaggggt taaagtgagt aaaattgtaa acttgcataa tgatattgca
     5581 ttagctttag cagcaaaaga tgttagaatt gaagcgccaa tacctggtcg ctctgcagta
     5641 ggtattgaag tgccaaatga gaaaatttca ttagtttcac taaaagaagt tttagatgaa
     5701 aaattcccgt ctaataataa actagaagtt ggattaggaa gagatatatc aggtgatcca
     5761 attactgttc cactaaatga aatgccacac ttattggtgg caggatcgac gggtagtggt
     5821 aaatctgttt gtataaatgg tattattaca agtattttat taaatgctaa gccgcatgaa
     5881 gttaaactta tgttaatcga tccgaaaatg gttgaactaa atgtttataa cggaattcca
     5941 catttattaa ttccggttgt tacaaatcct cataaagctg ctcaagcttt agaaaaaatt
     6001 gtagctgaga tggaaagacg ttatgattta ttccaacatt catcaactag aaatattaaa
     6061 ggttataacg aattaatccg taagcaaaat caagaattag atgagaagca accagaatta
     6121 ccttatatcg ttgttattgt agatgagctt gcagatttaa tgatggtagc tggtaaagaa
     6181 gttgaaaatg cgattcaacg tatcacacaa atggcacgtg cagcaggtat acatttgatt
     6241 gtagcgacac aaagaccttc tgtggatgta attacaggta tcattaaaaa taacattcca
     6301 tctagaattg cttttgctgt gagttctcaa acagattcaa gaactattat tggtactggc
     6361 ggcgcagaaa agttacttgg taaaggtgac atgttatacg ttggaaatgg tgattcatca
     6421 caaacacgta ttcaaggggc gtttttaagt gaccaagagg tgcaagatgt tgtaaattat
     6481 gtagtagaac aacaacaggc aaattatgta aaagaaatgg aaccagatgc accagtggat
     6541 aaatcggaaa tgaaaagtga agatgcttta tatgatgaag cgtatttgtt tgttgttgaa
     6601 caacaaaagg caagtacatc attgttacaa cgccaattta gaattggtta taatagagca
     6661 tctaggttga tggatgattt agaacgcaat caggtaatcg gtccacaaaa aggaagcaag
     6721 cctagacaag ttttaataga tcttaataat gacgaggtgt aaaaaaatgt cagaaatgaa
     6781 tgcggtatat aacgttaaac aatacatttt aaatttgatt aagcaaaata aattggaata
     6841 tggtgaccaa cttccaagta atttatcaat tgccagagaa ttaaatgtaa aaaccgacga
     6901 tgtttatgaa gcaattcagg cattgattac tgaacaagtc attaaagata attttgaaga
     6961 gggcacaagt gttaagtcac tgcccccttt cttttatcca ttgaatgaac ttataagtat
     7021 tgggcaaatg attaaaaatg caggatttga atgcggaact gaatacttaa attttgatga
     7081 gcaaccagca actatgttag atgcaaattt gttgagtgtt gaagaaggat atccagtaac
     7141 cattatagaa cgattacgaa ctgccgatgg agaaccggtc gtctattgtt tagataaaat
     7201 tgctaaaaaa gaattaacat gtacagagta tcaaatgagc aatggatcga tactaagtgc
     7261 gataaaagaa caaagtaatc ataatatttg ttacgctgat acagaaattg aagcggtaaa
     7321 ttatgaacct cgaatatccg aagtactgaa tgcttcgcca cacgaaggtt tgattttatt
     7381 aaaaattacg cactacaatg aatcagatga acctattttg tattcattaa attatatgaa
     7441 aaatagctta gttcaattta aaatcactag aaaaatatag atgtaagaag atatagagga
     7501 ggctatatat ttgagtagac aatctcaacc aaatatacat atcaaagttt caccaacaac
     7561 taaatttaaa acaactacta tagtttttaa atttatggca cctttagaat atgacacaat
     7621 aacagctaga tcattattaa gcaaattatt agttcgagca actaagaaat ggccaaccga
     7681 taagtcattt aataatcact tagccgattt atatggtgcg tatgtgaata gtacaatttc
     7741 aaaattcaaa gatcagcatg tcattacatt ttcattagaa attgttaatg aacgttattt
     7801 aagaaacggt gaatcattat ttaatcaagg attagattta ttacaagaaa tcatttggaa
     7861 tccattaatt gaaaataaag cattcaatga taattttgtt aaccaagaga aaacattatt
     7921 agccaaaaaa atagaagcaa tggtagataa caaagcacaa tattcgtttt taaaattact
     7981 cgaccatatg tttgaaaatg aagcatataa atacttatct acaggacaac tagaacaaat
     8041 cccacatatt actgctgaaa cactatatca tacatatcaa tcaatgatta ataatgatca
     8101 atgttctgtt tatgttgtcg gcaatgtaga acctgaaagt gttgagaaac aaatacgtga
     8161 aaaatttgca cttaaaccat tcgataaaca tcaattccaa cattctactc atcatttaca
     8221 cgatgaagaa gttgattata ttgttgaata tgatgacgtg gatcaagcta aattaaatat
     8281 gggataccga tttccaacac aatatggaca aagtggatat gctgcctttg ttgtatttaa
     8341 catgatgttt ggaggagatc cttcatctgt tttatttaat gaagtgcgag aaaagcaaag
     8401 tttagcgtac tctatacatt cacaaattga tggcaaaaat ggctatttat ttgttttgag
     8461 tggggtttca agcgataagt acgaaactgc aaaagacact attataagtg aatttgaaaa
     8521 aataaaagca ggagatttca ctgaagaaaa attagagtta gctaaaaaag taatcatttc
     8581 tcatcgatat gaatctgaag atcgtccgaa aagtattata gagattatgc ataaccaaat
     8641 attattagag caaccacaaa gcaaagaaac atttataaat gatatacaga aggtaagtcg
     8701 cgaagatatt gtttctgttg ctgaaaaagc atttttagat acaatctatg tgttgacaaa
     8761 aggaggggat aaataatgaa agagcgttat tatgaattaa tagacgaaag agtattcgaa
     8821 caagaattag aaaatggttt acgattattt attatcccca aaccaggttt tcaaaagaca
     8881 tttgtcactt acactacaca atttggttca ttagataatc aattcaaacc ccttggacaa
     8941 gaccaatttg ttactgtacc tgatggagtt gctcactttt tagaacataa attatttgaa
     9001 aaagaagaag aagacttatt tactgcgttt gctgaagaaa atgcacaagc aaatgcgttt
     9061 acaagctttg atcgtacaag ctacttgttc agtgcaactg ataatattga aaacaacatt
     9121 aaacgtttac ttacaatggt tgaaacgcct tattttacaa aagaaactgt tgataaagaa
     9181 aaaggtatta ttgcagaaga aataaaaatg tatcaagaac aacctggata taaattaatg
     9241 tttaatacat tgcgtgcaat gtatcaacaa catccaatac gtgttgatat tgccggtagt
     9301 gtagaaagta tatacgatat tacaaaagat gatttgtatc tatgttatga aacgttttat
     9361 catccatcaa atatggtttt atttgttgtt ggtgatgtgg atcctgaagc aatatgtcga
     9421 atagtaaaac aacacgagga tgctcgtaat aaagttaacc aacccaaaat cgaacgagga
     9481 cttgttgatg aaccggagga tgttaaagaa gcatttgtta ctgaatctat gaaaattcaa
     9541 tcaccaagac taatgcttgg ttttaaaaat aaaccattac aagaagcgcc tcaaaaatat
     9601 gtacaacgtg atttagaaat gtcattattc tttgagttaa tttttgggga agaaacagat
     9661 ttttatcaga atttattaaa cgaaggactt atcgatgata catttggtta tcaatttgta
     9721 ctagagccga cgtatagttt ttcaatcgtg acaagcgcta ctgaagaacc agataaatta
     9781 aaaaaattat tattagatga gttgcgcgat aaaaaaggca atttccaaga tgcagaagca
     9841 tttgaacttt tgaaaaagca atttataggt gaattcatat caagtttaaa ctcacctgaa
     9901 tatattgcta atcaatatac taaattgtat tttgaaggtg ttagtgtgtt tgacatgtta
     9961 gacattgttg aaaacatcac gttagatagt attaatgaaa cgtcatcgtt atatttaaat
    10021 ttagatcagc aagtcgatag tcgtttggag attaaaaagt aatgaaagca ttagtattag
    10081 gtggttctgg ttcaattggt tctgagatag tcaaacaatt attaactgat ggatttgaag
    10141 tttatgtgca atattatcgt actgatataa atgaattaac taacaaattt aatgatgata
    10201 aagttcattt tatacaagcg gatttatctc aaacaattga tattgacaaa acatttggtg
    10261 acattaaatc attagactgt ttaatatatg caagtggtca gtctttatat ggtgttttac
    10321 aagatatgaa agaccatgat attgatgcat gttatcagtt aaatgtcttg caattaattc
    10381 gattatgtag atatttcgtt gatgttttac gtcaaagtga caatggaaga attattgtaa
    10441 tttcatcaat ttggggtgag acaggagcta gcatggaaac tatttattcg acgatgaaaa
    10501 gtgcacaatt aggtttcgtt aaggcgctta gtcaagagct tgcactaaca tcagtgacag
    10561 taaatgctat cgcacctggt tttgtagctg gtaatatggc aagtgagtgg caagaagatg
    10621 aacttcaagc aatgataact gaattaccac aacagcgatt ggttttaccg agtgaggttg
    10681 ctcatacatg cgcctattta tatcacccaa atgctagaag tgttactgga actatacaga
    10741 aagttaatgg ggcttggtat atttaaaata tagaactatt tttatgagca aatgttttta
    10801 tgattgatgt tataatatga atattacaat gaatcataat ttgttaatta aacaaggggt
    10861 gaggttcacc atgacagttg cagagaaaaa agaatggtac ctagaatacg aaattgaaat
    10921 taatagaccg ggtcttttag gtgatgtatc tagtttatta ggtatgttag gtataagtat
    10981 tgttacaatt aatggtgttg atcaaggtaa acgaggcctt ttaattaaaa cagacaatct
    11041 tgaaaaagtt gaacgttttg agcaaatagc tcgtggtata aatgaaattg aaataacaaa
    11101 gcttaaaaaa ccagaattaa gagaccgtct tgcagttaga catggtagat atattgagca
    11161 agatgcaaaa gataagaaaa cctttcgatt tgagcgtgaa gatttaggct tgttagtaga
    11221 ctttttagct gaattgttca aagaagaagg tcataagttg attggcatta gaggtatgcc
    11281 acgggttggt aaaaccgaat caattgttgc gggaagtgtt tgtgcacata agagatggtt
    11341 atttattagt tctactttaa taaaacaaac tgtacgtagc tctctaatta aaggggaata
    11401 tgatgccaat catgtatata ttattgatgg tgcagttact gccagagaat ctaatccaaa
    11461 acatcaagag cttgttaacg aagttatgac gttaccttca atcaaagtcg ttgaacaccc
    11521 agatttattt gttgaaacaa gtacttgtac aatggaagat tttgactata ttatcgaatt
    11581 gagagaaaat gaaaatcaag aaatacatta cgaagaaatg aagaaacaga cagtccaaag
    11641 taagaataat ttagattttg gagatccatt tggtggtggt tttggtttct tcgagtaagt
    11701 gtaaggaggc tatgaattga aaacggtcgg tgaagcgcta aaaggtagac gtgaaaggtt
    11761 aggaatgact ttaacagaat tagagcaacg tactggaatt aaacgtgaaa tgctagtgca
    11821 tattgaaaat aatgaattcg atcaactacc gaataaaaat tacagcgaag gatttattag
    11881 aaaatatgca agcgtagtaa atattgaacc taaccaatta attcaagctc atcaagatga
    11941 aattccatcg aaccaagccg aatgggacga agtaattaca gttttcaata ataataaaga
    12001 cttagattat aagagtaaat caaaagagcc aatacaatta ttagtaatca tgggtattac
    12061 agttttaata actttattgt tatggatcat gttagtttta atattttaac agaaataaat
    12121 tagtgagaaa tgaggatgta taatgaatat tccgaaccag attacggttt ttagagtagt
    12181 gttaatacca gtttttatat tgtttgcgtt agttgatttt ggatttggca atgtgtcatt
    12241 tctaggagga tatgaaataa gaattgagtt attaatcagt ggttttattt ttatattggc
    12301 ttcccttagc gattttgttg atggttattt agctagaaaa tggaatttag ttacaaatat
    12361 ggggaaattt ttggatccat tagcggataa attattagtt gcaagtgctt taattgttct
    12421 tgtgcaacta ggactaacca attctgtagt agcaatcatt attattgcca gagaatttgc
    12481 cgtaactggt ttacgtttac tacaaattga acaaggattt gtaagtgcag ctggtcaatt
    12541 aggtaaaatt aaaacagcag ttactatggt agcaattact tggttgttat taggtgatcc
    12601 attggcaaca ttgattggtt tgtcattagg acaaatttta ttatacattg gcgttatttt
    12661 tactatctta tctggtattg aatactttta taaaggtaga gatgttttta aacaaaaata
    12721 aatatttgtt tatactagat ttcattttca tatggaatct agttttttta atcccaattt
    12781 tagaaattag ccacgcattt gtttataatg atatattgta aaataatatt tgttcatttt
    12841 ttagggaaat gtgttgtagc atctgataca ttgaatttaa aattgatgtg aatttttaaa
    12901 tggaatacat gaaaaaatga attaaacgat acaaggggga tataaatgtc aattgccatt
    12961 attgctgtag gctcagaact attgctaggt caaatcgcta ataccaatgg acaatttcta
    13021 tctaaagtat ttaatgaaat tggacaaaat gtattagaac ataaagttat tggagataat
    13081 aaaaaacgtt tagaatcaag tgttcgtcat gcgttagaaa aatatgatac tgttatttta
    13141 acaggtggct taggtcctac gaaagatgac ttaacgaagc atacagtggc ccagattgtt
    13201 ggtaaagatt tagttattga tgagccttct ttaaaatata ttgaaagcta ttttgaggaa
    13261 caaggacaag aaatgacacc taataataaa caacaggctt tagtaattga aggttcaact
    13321 gtattagcaa atcatcatgg catggctcca ggaatgatgg tgaattttga aaacaaacaa
    13381 attattttat taccaggtcc accgaaagaa atgcaaccaa tggtaaaaaa tgaattgttg
    13441 tcacatttta taaaccataa tcgaattata cactctgaac tattaagatt tgctggaata
    13501 ggtgaatcta aagtagaaac agtattaata gatcttatcg ataaacagac taatcctacg
    13561 attgcgcctt tggcaggaag tcatgaagta tatattagat tgactgcaaa tgccgactca
    13621 aaagaacaag cacaatcatt gattcaacct gttaagcaag aaattcttga tcgtattgga
    13681 gaatattatt atggttcaga tgacacatta attgagcaag ctgtaataaa gaaaattaat
    13741 gaaccttttg taatatatga tggtattact aatggtgctt tatatcatcg attgaaagaa
    13801 gtggatttaa acgatgttct aaagggtatg attaatcaca atgaaaactt tgttgatatt
    13861 aataaaccta ttgagcagca attaaaagat gcagtgcaat ttgttaataa attatttaat
    13921 gtgtcatcag caattattct attagagtat gatggtgtag tccatatagg ctatgataat
    13981 aacttagaat ttaaaactga gcaatttaaa atgtctgaat ctagaaattt attaaagaac
    14041 agaagtcaaa attatgcgct tataaggtta ttaaattggc ttagaacaac aaattaattg
    14101 tattatcgat aaaaatataa gcacgtttgt tcgtttttgt gttttgattt caagatttta
    14161 cacgaacaaa tattcgtaaa acacttgtat tttattttga atccttgtat agtattggta
    14221 agataattaa aagatagcaa tttcaattag gaggtctcgc tttggataac gatcgtcaaa
    14281 aagctttaga tacagtaatt aaaaatatgg agaaatcttt cggtaaaggt gccgtaatga
    14341 agttgggtga caatataggt cgccgagttt caactacatc aactggttca gttacattag
    14401 ataatgcgct aggtgtaggt ggctatccta aaggacgaat tattgaaatt tatggtcctg
    14461 aaagttctgg taagacaaca gtagcgcttc acgctattgc tgaagtacaa agtaatggcg
    14521 gggtggcagc atttatcgat gctgaacatg ctttagatcc agaatatgct caagcattag
    14581 gcgtagatat cgataattta tatttatcgc aaccggatca tggtgaacaa ggtcttgaaa
    14641 tcgctgaagc atttgttaga agtggtgcag ttgatattgt agttgtagac tcagttgctg
    14701 ctttaacacc taaagctgaa attgaaggag aaatgggaga cactcacgtt ggtttacaag
    14761 ctcgtttaat gtcacaagcg ttacgtaaac tttcaggtgc tatttctaaa tcaaatacaa
    14821 ctgctatttt catcaaccaa attcgtgaaa aagttggtgt tatgttcggt aatccagaga
    14881 ctacaccagg tggacgtgca ttaaaattct atagttcagt aagactagaa gtacgtcgtg
    14941 cagaacagct taaacaagga caagaaattg taggtaatag aactaaaatt aaagtcgtta
    15001 aaaataaagt ggcaccacca tttagagtag ctgaagttga tattatgtat ggacaaggta
    15061 tttctaaaga gggtgaactt attgatttag gtgttgaaaa cgacatcgtt gataaatcag
    15121 gagcatggta ttcatacaat ggcgaacgaa tgggtcaagg taaggaaaat gttaaaatgt
    15181 acttgaaaga aaatccacaa attaaagaag aaattgatcg taaattgaga gaaaaattag
    15241 gtatatctga cggtgatgtt gaagaaacgg aagatgcacc aaagtcatta tttgacgaag
    15301 aatagtacac aaatttatat ctatagttaa acttagcaaa tatccttata ggattgattg
    15361 aaagtgatat tcatctcata aagctggaat aatatctaac tttatgggat gcacttcaaa
    15421 tcgagactat aaggtttttt attttattta tttttacatt atcaatagtt ttataatcga
    15481 gcttcaaaac tttagaaaat agtagaaata gcattcaata tagtgcaaaa gtgcaaattg
    15541 ataacttgac acttatctcc tataaaccgt acaattaatt tgtatgattt atatataatt
    15601 tcataaagtc atattgaatt tcatataaag agcaaaccct agaaaaggag gtgtttgtgt
    15661 gaatttatta agcctcctac tcattttgct ggggatcatt ctaggagttg ttggagggta
    15721 tgttgttgcc cgaaatttgt tgcttcaaaa gcaatcacaa gctagacaaa ctgccgaaga
    15781 tattgtaaat caagcacata aagaagctga caatatcaaa aaagagaaat tacttgaggc
    15841 aaaagaagaa aaccaaatcc taagagaaca aactgaagca gaactacgag aaagacgtag
    15901 cgaacttcaa agacaagaaa cccgacttct tcaaaaagaa gaaaacttag agcgtaaatc
    15961 tgatctatta gataaaaaag atgagatttt agagcaaaaa gaatcaaaaa ttgaagaaaa
    16021 acaacaacaa gtagatgcaa aagagagtag tgttcaaacg ttaataatga agcatgaaca
    16081 agaattagaa cgcatctccg gtctcactca agaagaagct attaatgagc aacttcaaag
    16141 agtagaggaa gaactgtcac aagatattgc agtacttgtt aaagaaaaag aaaaagaagc
    16201 taaagaaaaa gttgataaaa cagcaaaaga attattagct acagcagtac aaagattagc
    16261 agcagatcac acaagtgaat caacggtatc agtagttaac ttacctaatg atgagatgaa
    16321 aggtcgaatc attggacgtg aaggacgaaa catccgtaca cttgaaactt taactggcat
    16381 tgatttaatt attgatgaca caccagaagc agttatatta tctggttttg atccaataag
    16441 aagagaaatt gctagaacag cacttgttaa cttagtatct gatggacgta ttcatccagg
    16501 tagaattgaa gatatggtcg aaaaagctag aaaagaagta gacgatatta taagagaagc
    16561 aggtgaacaa gctacatttg aagtgaacgc acataatatg catcctgact tagtaaaaat
    16621 tgtagggcgt ttaaactatc gtacaagtta cggtcaaaat gtacttaaac attcaattga
    16681 agttgcgcat cttgctagta tgttagctgc tgagctaggc gaagatgaga cattagcgaa
    16741 acgagctgga cttttacatg atgttggtaa agcaattgat catgaagtag aaggtagtca
    16801 tgttgaaatc ggtgtagaat tagcgaaaaa atatggtgaa aatgaaacag ttattaatgc
    16861 aatccattct caccatggtg atgttgaacc tacatctatt atatctatcc ttgttgctgc
    16921 tgcagatgca ttgtctgcgg ctcgtccagg tgcaagaaaa gaaacattag agaattatat
    16981 tcgtcgatta gaacgtttag aaacgttatc agaaagttat gatggtgtag aaaaagcatt
    17041 tgcgattcag gcaggtagag aaatccgagt gattgtatct cctgaagaaa ttgatgattt
    17101 aaaatcttat cgattggcta gagatattaa aaatcagatt gaagatgaat tacaatatcc
    17161 tggtcatatc aaggtgacag ttgttcgaga gactagagca gtagaatatg cgaaataatt
    17221 tttgtctccc tcacaaatta gtgagggagc ttttttaagt tgtagtttca atctagttag
    17281 acagtacttt attggtaata actatattag acagtagtta tttgaaagta agatggacct
    17341 tatattaaat aagaagttat tgcttttaat aaaaatgttt taggttcata attactatat
    17401 ttatattatg taaacctata aaggtgattg ctttttcaaa ctaaaaaaaa gaagagaaga
    17461 tgtaacacat cttctcttct gtaatattaa ttagaaatta tttctaagtt gagttatttt
    17521 aattgtaaat ctgttttctt taattctttt ataacttctg cagtatcata acaatttgtt
    17581 gcaattgttg aatatctctc tgctaaacga tatgcattaa tgtaaagctt taaactttct
    17641 ttagctatat cctctgcatc ttcgaatttt gatgggttag acataaccac taattctgca
    17701 aatttttctg gatcaatatt aatagacatg tatttattta caactcctat ttattttgat
    17761 gtcttaatac taacatattg aagtttt
//
LOCUS       DAKSQE010000053        17638 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.053, whole genome shotgun sequence.
ACCESSION   DAKSQE010000053 DAKSQE010000000
VERSION     DAKSQE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17638
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.053"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            331..1101
                     /locus_tag="O0V33_001483"
     CDS             331..1101
                     /locus_tag="O0V33_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCX8976114.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRVASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSY
                     SEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            1108..1890
                     /locus_tag="O0V33_001484"
     CDS             1108..1890
                     /locus_tag="O0V33_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCX8976115.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
     gene            2102..3388
                     /gene="rseP"
                     /locus_tag="O0V33_001485"
     CDS             2102..3388
                     /gene="rseP"
                     /locus_tag="O0V33_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCX8976116.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGI
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTERKLTK
                     VSSETKYVLGFQPASEHTLFKPIVYGFKSFLIGSTYIFSAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            3408..5111
                     /locus_tag="O0V33_001486"
     CDS             3408..5111
                     /locus_tag="O0V33_001486"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCX8976117.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKAGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKHTTVQPIEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQPLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
     gene            5375..9685
                     /locus_tag="O0V33_001487"
     CDS             5375..9685
                     /locus_tag="O0V33_001487"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCX8976118.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKSKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEYKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGETLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
     gene            9975..10442
                     /gene="rimP"
                     /locus_tag="O0V33_001488"
     CDS             9975..10442
                     /gene="rimP"
                     /locus_tag="O0V33_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCX8976119.1"
                     /translation="MSKITEQVEVIVKPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            10463..11638
                     /gene="nusA"
                     /locus_tag="O0V33_001489"
     CDS             10463..11638
                     /gene="nusA"
                     /locus_tag="O0V33_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCX8976120.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTVVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDVALEDADTTESTEEVTDVSVETNVEKESE"
     gene            11659..11943
                     /locus_tag="O0V33_001490"
     CDS             11659..11943
                     /locus_tag="O0V33_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCX8976121.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            11940..12257
                     /locus_tag="O0V33_001491"
     CDS             11940..12257
                     /locus_tag="O0V33_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HCX8976122.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SNNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            12262..14370
                     /gene="infB"
                     /locus_tag="O0V33_001492"
     CDS             12262..14370
                     /gene="infB"
                     /locus_tag="O0V33_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCX8976123.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKKNNRNNKK
                     NNKNNKPQSQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVANINQ
                     SLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHVDHG
                     KTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARGAQV
                     TDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTEYGL
                     IPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAELDKS
                     RGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGINDVP
                     QAGDRFVVFSDEKQARRIGESRHEASIVQQRQESKNVSLDNLFEQMKQGEMKDLNVII
                     KGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNVRPD
                     SGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKVSKV
                     GTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGITIEN
                     YNDLKEGDVIEAFEMVEIKR"
     gene            14757..15107
                     /gene="rbfA"
                     /locus_tag="O0V33_001493"
     CDS             14757..15107
                     /gene="rbfA"
                     /locus_tag="O0V33_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCX8976124.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
     gene            15277..16194
                     /gene="truB"
                     /locus_tag="O0V33_001494"
     CDS             15277..16194
                     /gene="truB"
                     /locus_tag="O0V33_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCX8976125.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLEMKGVHSANFNNDDI
                     DRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHPTKESEIKPKKVFN"
     gene            16209..17180
                     /gene="ribF"
                     /locus_tag="O0V33_001495"
     CDS             16209..17180
                     /gene="ribF"
                     /locus_tag="O0V33_001495"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCX8976126.1"
                     /translation="MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVNKQEIEN
                     EKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYL
                     LPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERV
                     TVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            17295..17564
                     /gene="rpsO"
                     /locus_tag="O0V33_001496"
     CDS             17295..17564
                     /gene="rpsO"
                     /locus_tag="O0V33_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCX8976127.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
BASE COUNT         6406 a         2374 c         3459 g         5399 t
ORIGIN      
        1 taatgtaaca tttaaaatgg gcatgtttaa ttaaatcaaa gatgcatgtg ataatttaaa
       61 ttcacaatga gcataaaaat ggtgtttaaa caagttaatt aaacatatac tttataaata
      121 ataggcatta ggtatattga tataataaag ttatgtaatt tttaacctca gtatgtatgt
      181 cacatttctg gtgtaaactg taccgagtca gactttggta cagttttttt atttgcttat
      241 tcaatgcatt aaatgagtat gataaaatga taatgattgt ttagtaactt atactatatg
      301 acagagatga tcaggctcgg aggaaagacc atgtttaaaa agctaataaa taaaaagaac
      361 actataaata attataatga agaattagac tcgtctaata tacctgaaca tatcgctatt
      421 attatggatg gtaatgggcg atgggctaag aagcgaaaaa tgcctagaat taaaggtcat
      481 tacgaaggta tgcaaacaat aaaaaaaatt actagggtag ctagtgatat tggtgttaag
      541 tacttaactt tatatgcctt ttccactgaa aattggtcaa gacctgaaag tgaagtaaat
      601 tatattatga atttgcctgt caatttctta aagacatttt taccggaact aattgaaaaa
      661 aatgtcaaag ttgaaacaat tggatttact gataagttgc caaaatcaac gatagaagca
      721 ataaataatg ctaaagaaaa gacagctaat aataccggct taaaattaat atttgcaatt
      781 aattatggtg gcagagcaga acttgttcat agtattaaaa atatgtttga cgagcttcat
      841 caacaaggtt tgaatagtga tatcatagat gaaacatata taaacaatca tttaatgaca
      901 aaagactatc ctgatccaga gttgttaatt cgtacttcag gagaacaaag aataagtaat
      961 ttcttgattt ggcaagtttc gtatagtgaa tttatcttta atcaaaaatt atggcctgac
     1021 tttgacgaag atgaattaat taaatgtatt aaaatttatc agtcacgtca aagacgcttt
     1081 ggcggattga gtgaggagta gtatagtatg aaagttagaa cgctgacagc tattattgcc
     1141 ttaatcgtat tcttgcctat cttgttaaaa ggcggccttg tgttaatgat atttgctaat
     1201 atattagcat tgattgcatt aaaagaattg ttgaatatga atatgattaa atttgtttca
     1261 gttcctggtt taattagtgc agttggtctt atcatcatta tgttgccaca acatgcaggg
     1321 ccatgggtac aagtaattca attaaaaagt ttaattgcaa tgagctttat tgtattaagt
     1381 tatactgtct tatctaaaaa cagatttagt tttatggatg ctgcattttg cttaatgtct
     1441 gtggcttatg taggcattgg ttttatgttc ttttatgaaa cgagatcaga aggattacat
     1501 tacatattat atgccttttt aattgtttgg cttacagata caggggctta cttatttggt
     1561 aaaatgatgg gtaaacataa gctttggcca gtaataagtc cgaataaaac aatcgaagga
     1621 ttcataggtg gcttgttctg tagtttgata gtgcctcttg caatgttata ttttgtagat
     1681 ttcaatatga atgtatggat attacttgga gtgacattga ttttaagttt atttggtcaa
     1741 ttaggtgatt tagtggaatc aggatttaag cgtcatttcg gcgttaaaga ctcaggtcga
     1801 atactacctg gacacggtgg tattttagac cgatttgaca gttttatgtt tgtgttacca
     1861 ttattaaata ttttattaat acaatcttaa tgctgagaac aaatcaataa acgtaaagag
     1921 gagttgctga gataatttaa tgaatctcag aactcctttt gaaaattata cgcaatatta
     1981 actttgaaaa ttagacgtta tattttgtga tttgtcagta tcatattata atgacttatg
     2041 ttacgtatac agcaatcatt tttaaaataa aagaaattta taaacaatcg aggtgtagcg
     2101 agtgagctat ttagttacaa taattgcatt tattattgtt tttggtgtac tagtaactgt
     2161 tcatgaatat ggccatatgt tttttgcgaa aagagcaggc attatgtgtc cagaatttgc
     2221 gatcggtatg gggccgaaaa tttttagttt tagaaaaaat gaaacacttt acactattag
     2281 gttattgcct gttggtggat atgttcgtat ggcaggagat ggcttagaag agccaccagt
     2341 cgagcctggt atgaacgtta aaattaaact gaatgaagaa aatgaaataa cacatatcat
     2401 attagatgat catcataagt ttcaacaaat tgaagcgatc gaagttaaaa aatgtgattt
     2461 taaagatgac ttattcatag aaggtatcac tgcttatgat aatgaaagac atcattttaa
     2521 aattgctaga aagtctttct ttgttgaaaa tggtagctta gttcaaattg ctccgagaga
     2581 cagacaattt gcacataaaa agccatggcc gaaattttta acattatttg cgggaccgtt
     2641 atttaacttt atattagctt tagttctatt tattggtctt gcatattatc aaggtacacc
     2701 tacttctact gtagaacaag tcgcagataa gtatccagct caacaagcag gcatacaaaa
     2761 aggcgataaa attgtccaaa ttggcaaata taaaatatct gaatttgatg atgttgataa
     2821 ggcgttagat aaagttaaag ataataagac gactgttaaa tttgaacgtg atggtaaaac
     2881 aaagtcagtt gaattaacac ctaaaaagac cgaaagaaaa ctgactaaag taagttcaga
     2941 gacgaagtat gttctcggat tccaaccagc gagtgaacat acactcttca aaccaattgt
     3001 atatggattt aaaagctttt taatcggtag tacttatatt ttttcagctg tagtaggtat
     3061 gttggctagt atatttacgg gcggattctc gtttgatatg ttaaatggtc cggttggtat
     3121 ttatcataac gtcgactcag ttgttaaagc gggtatcatt agcttaattg gatacactgc
     3181 gttattaagt gtaaacttag gtattatgaa tttaattcct attcctgcac tagacggtgg
     3241 tcgtatttta tttgttatat atgaagcgat tttcagaaaa ccagttaata aaaaagcgga
     3301 aacaacgatt atcgctattg gtgccatttt catggtcgtt attatgatat tagtaacgtg
     3361 gaatgatatt cgacgatatt tcttataatt taggaggata aataattatg aagcaatcca
     3421 aagtttttat accaacgatg cgtgacgtgc catcagaagc agaagcacaa agtcatcgtt
     3481 tattattgaa agcgggtttg ataaaacaaa gtacaagtgg gatttatagt tatttaccgc
     3541 tagcaacacg tgtgttaaat aatattactg caattgtgcg acaagaaatg gaacgtatcg
     3601 attctgttga aattttaatg ccagcgttac aacaagctga attatgggaa gaatcaggac
     3661 gttggggtgc atatggccca gaattaatgc gtttacaaga tagacatgga agacaatttg
     3721 cattaggtcc aacacatgaa gaattagtta catcaatagt aagaaatgaa ttgaaatcat
     3781 acaaacaatt accgatgaca ttattccaaa ttcaatctaa attccgtgat gaaaagagac
     3841 cacgttttgg tttacttcgt ggtcgtgaat ttattatgaa agatgcgtat tcattccatg
     3901 ctgacgaggc atcattagat caaacgtatc aagatatgta tcaagcgtat agccgtattt
     3961 ttgagagagt tggcattaac gcaagaccag tagttgcaga ttcaggtgct attggcggta
     4021 gccatacaca tgaatttatg gcattaagtg ctatcggtga ggatacaatc gtttacagta
     4081 aagaaagtga ctatgctgct aacatcgaaa aagcagaagt cgtttacgaa ccaaatcata
     4141 agcatactac tgtgcaacct atagaaaaaa ttgaaacacc aaatgttaag actgcgcaag
     4201 aattggcaga cttcttaggt agaccagtag atgaaatcgt taaaacgatg attttcaaag
     4261 ttgatggcga atatattatg gttttagtgc gtggacatca tgaaattaat gacattaaat
     4321 taaaatctta tttcggcaca gataatattg aattagcaac acaagacgaa attgttaatt
     4381 tagttggtgc aaatcctggt tcactaggtc ctgtaattga taaagagatc aaaatttatg
     4441 cagataattt tgtgcaagat ttaaataatt tagttgtcgg ggctaacgaa gatggttatc
     4501 acttaattaa tgtaaatgta ggtagagact tcaacgttga tgaatatggc gatttccgtt
     4561 ttattttaga aggcgaaaag ttaagtgatg gttcaggcgt tgcacatttt gctgaaggaa
     4621 ttgaagttgg tcaagtattc aaattgggta ctaagtattc agaatcaatg aatgctacat
     4681 tcttagataa tcaaggaaaa gcccaaccgt taattatggg ttgttacggt attggaattt
     4741 ctagaacgct aagtgcgatt gttgaacaaa atcacgatga taatggtatt gtttggccta
     4801 aatcagttac tccatttgat ttacatttaa tttctattaa tcctaagaaa gatgatcaac
     4861 gagaactagc ggatgcacta tatgctgaat ttaatactaa atttgatgtg ttgtacgatg
     4921 atcgtcagga acgtgctggt gtcaaattta atgatgccga tttaattggt ttaccactgc
     4981 gaattgttgt tggtaaacgt gcatcggaag gtattgtaga agttaaagaa cgtttaacag
     5041 gtgatagcga agaagtccac attgatgact taatgactgt cattacaaat aaatatgata
     5101 atttaaaata attaagatcg aatgaattat aagagtagga aaaagctgaa agaaatcaga
     5161 tgcttatgtc ctgctcttat tatttttgat ataatgatta ttcgatgaaa aatgactgaa
     5221 gacatagtat aattaaagat aaatttgttt taacaatatt atgattagcc aaatataaag
     5281 catttaattt tctatcatta ctatgctcac ataatctaaa tattgttcga acacgtaaaa
     5341 gtaatttcta tttaaggtgg taattgtctt ggcaatgaca gagcaacaaa aatttaaagt
     5401 gcttgctgat caaattaaaa tttcaaatca attagatgct gaaattttaa attcaggtga
     5461 actgacacgt atagatgttt ctaacaaaaa cagaacatgg gaatttcata ttacattacc
     5521 acaattctta gctcatgaag attatttatt atttataaat gcaatagagc aagagtttaa
     5581 agatatcgcc aacgttacat gtcgttttac ggtaacgaat ggcacgaatc aagatgaaca
     5641 tgcaattaaa tactttgggc actgtattga ccaaacagct ttatctccaa aagttaaagg
     5701 tcaattgaaa cagaaaaagc ttattatgtc tggaaaagta ttaaaagtaa tggtatcaaa
     5761 tgacattgaa cgtaatcatt ttgataaggc atgcaatgga agtcttatca aagcgtttag
     5821 aaattgtggt tttgatatcg ataaaatcat attcgaaaca aatgataatg atcaagaaca
     5881 aaacttagct tctttagaag cacatattca agaagaagac gaacaaagtg cacgattggc
     5941 aacagagaaa cttgaaaaaa tgaaagctga aaaatcgaaa caacaagata acaacgaaag
     6001 tgctgtcgat aagtgtcaaa ttggtaagcc gattcaaatt gaaaatatta aaccaattga
     6061 atctattatt gaggaagagt ataaagttgc aatagagggt gtcatttttg atattaactt
     6121 aaaagaactt aaaagtggtc gtcatatcgt agaaattaaa gtgactgact atacggactc
     6181 tttagtttta aaaatgttta ctcgtaaaaa caaagatgat ttagaacatt ttaaagcgct
     6241 aagtgttggt aaatgggtta gggctcaagg tcgtattgaa gaagatacat ttattagaga
     6301 tttagttatg atgatgtctg atattgaaga gattaaaaaa gcgacaaaaa aagataaggc
     6361 tgaagaaaaa cgtgtagaat tccacttaca tactgcaatg agtcaaatgg atggtatacc
     6421 aaatattggt gcgtatgtta aacaggcagc agactgggga catccagcca ttgcggttac
     6481 agaccataat gttgtgcaag catttccaga tgctcacgca gcagcggaaa aacatggtat
     6541 taaaatgata tatggtatgg aaggtatgtt agttgatgat ggtgttccga ttgcatacaa
     6601 accacaagat gtcgtattaa aagatgctac ttatgttgta ttcgacgtcg agacaactgg
     6661 tttatcaaat cagtatgata aaatcatcga gcttgcagct gtgaaagttc ataacggcga
     6721 aatcatcgat aagtttgaaa ggtttagtaa tccgcatgaa cgattatcgg aaacgattat
     6781 caatttgacg catattactg atgatatgtt agtagatgcc cctgagattg aagaagtact
     6841 tacagaattt aaagaatggg ttggcgatgc gatattcgta gcacataatg cttcgtttga
     6901 tatgggcttc atcgacacgg gatatgaacg tcttggattt ggaccatcta cgaatggtgt
     6961 tatcgataca ttagaattat ctcgtacgat taatactgaa tatggtaaac atggtttaaa
     7021 tttcttagct aaaaaatacg gtgtagaatt aacgcaacat caccgtgcca tttatgatac
     7081 agaggcaaca gcttacattt tcataaaaat ggttcaacaa atgaaagaat taggcgtatt
     7141 aaatcataac gaaattaaca aaaaactcag taatgaagat gcatataaac gtgcaagacc
     7201 tagtcatgtc acattaattg tacaaaatca acaaggtctt aaaaatctat ttaaaattgt
     7261 aagtgcatca ttggtgaagt atttctaccg tacgcctcga attccacgtt cattgttaga
     7321 tgaatatcgt gagggattat tggtaggtac agcgtgtgat gaaggtgaat tatttacggc
     7381 agttatgcag aaggaccaga gccaagttga aaaaattgcc aaatattatg attttattga
     7441 aattcaacca ccggcacttt atcaagattt aattgataga gagcttatta gagatactga
     7501 aacattacat gaaatttatc aacgtttaat tcatgcaggt gacacagcgg gtattcctgt
     7561 tattgcgaca ggaaatgcac actatttgtt tgaacatgat ggcatcgcac gtaaaatttt
     7621 aatagcatca caacccggca atccacttaa tcgctcaact ttaccggaag cacattttag
     7681 aactacagat gaaatgttaa acgagtttca ttttttaggt gaagaaaaag cgcatgaaat
     7741 tgttgtgaaa aatacaaacg aattagcaga tcgaattgaa cgtgttgttc ctattaaaga
     7801 tgaattatac acaccgcgta tggaaggtgc taacgaagaa attagagaac taagttatgc
     7861 aaatgcgcgt aaactgtatg gtgaagatct gcctcaaatc gtaattgatc gattagaaaa
     7921 agaattaaaa agtattatcg gtaatggatt tgcggtaatt tacttaattt cgcaacgttt
     7981 agttaaaaaa tcattagatg atggatactt agttggttcc cgtggttcag taggttctag
     8041 ttttgtggcg acaatgactg agattactga agtaaacccg ttaccgccac actatatatg
     8101 tccgaactgt aaaacgagtg aatttttcaa tgatggttca gtaggatcag gattcgattt
     8161 acctgataag acgtgtgaaa cttgtggagc gccacttatt aaagaaggac aagatattcc
     8221 gtttgaaaca tttttaggat ttaaaggaga taaagttcct gatatcgact taaactttag
     8281 tggtgaatat caaccaaatg cccataacta cacaaaagta ttatttggtg aggataaagt
     8341 attccgtgca ggtacaattg gtactgttgc tgaaaagact gcttttggtt atgttaaagg
     8401 ctatttgaat gatcaaggca tccacaaaag aggtgctgaa atagatcgac tcgttaaagg
     8461 atgtacaggt gttaaacgta caactggaca gcatccaggg ggtattattg tagtacctga
     8521 ttacatggat atttatgatt ttacgccgat acaatatcct gccgatgatc aaaattcagc
     8581 atggatgacg acacattttg atttccattc tattcatgat aatgtattaa aacttgatat
     8641 acttggacac gatgatccaa caatgattcg tatgcttcaa gatttatcag ggattgatcc
     8701 aaaaacaata cctgtagatg ataaagaagt tatgcagatt tttagtacac ctgaaagttt
     8761 gggtgttact gaagatgaaa ttttatgtaa aacaggtaca tttggggtac cagaattcgg
     8821 tacaggattc gtgcgtcaaa tgttagaaga tacaaagcca acaacatttt cagaattagt
     8881 tcaaatctca ggattatctc atgggacaga tgtgtggtta ggtaatgctc aagaattaat
     8941 taaaacaggt atatgtgatt tatcaagtgt aattggttgt cgtgacgata tcatggttta
     9001 tttaatgtat gctggtctag aaccatcaat ggcttttaaa ataatggagt cagtacgtaa
     9061 aggtaaaggt ttaactgaag aaatgattga aacgatgaaa gaaaatgaag tgccggattg
     9121 gtatttagat tcatgtctta aaattaagta catgttccct aaagcccatg cagcagcata
     9181 cgttttaatg gcagtacgta tcgcatattt caaagtacat catccacttt attactatgc
     9241 atcttacttt acaattcgtg cgtcagattt tgatttaatc acgatgatta aagataaaac
     9301 aagcatacga aatactgtaa aagacatgta ttctcgctat atggatctag gtaaaaaaga
     9361 aaaagacgta ttaactgtct tggaaattat gaatgaaatg gcgcatcggg gttatcgaat
     9421 gcaaccgatt agtttagaaa agagtcaagc gttcgaattt atcattgaag gcgaaacact
     9481 tattccgccg ttcatatcag tgcctgggct tggcgaaaac gttgcgaaac gaattgttga
     9541 agctcgtgac gatggaccat ttttatcaaa agaagattta aacaaaaaag ctggattatc
     9601 tcagaaaatt attgagtatt tagatgagtt aggctcatta ccgaatttac cagataaagc
     9661 tcaactttcg atattcgata tgtaaaatga aataatcaag gtatttattt aattcgtatg
     9721 gcgtagttaa agaaatacaa aattgttgct ggacacaaag ttatacctgt atttcttttc
     9781 aacgtcttac gagtctattc gaatgtaatg atgaaataaa tgaacaaact tttacaagaa
     9841 tctctgatta atagtgaagt catttgtttc aagcataaac ttatgctata attaagttgc
     9901 ttaaaaatta gtgaactcag gcagaagagt gggagattcc cgctcttttc tatttgccaa
     9961 aaagggaggc ctgtatgagt aaaattacag aacaagtaga agtgattgtt aaaccaatta
    10021 tggaagactt gaattttgaa cttgtagacg ttgaatatgt caaagagggt agagatcatt
    10081 ttcttagaat ctctattgat aaagaaggtg gcgtagattt aaatgattgt acgctagctt
    10141 ctgaaaaaat aagtgaagct atggatgcaa atgatcctat tcctgaaatg tattatttag
    10201 acgtagcgtc acctggtgca gaacgtccaa ttaaaaaaga acaagatttc caaaatgcaa
    10261 taactaaacc tgtatttgtt tctttatatg taccaattga aggtgaaaag gaatggttag
    10321 gcattttaca agaagtcaat aatgaaacaa ttgtagtaca agttaaaatc aaagcaagaa
    10381 cgaaagatat agagataccg agagacaaaa tagcaaaagc acgtcacgca gttatgattt
    10441 aacgtgatga ggaggaaaaa acgtgtcaag taatgaatta ttattagcta ctgagtattt
    10501 agaaaaagaa aagaagattc ctagagcagt attaattgat gctattgaag cagctttaat
    10561 tactgcatac aaaaagaatt atgatagtgc aagaaatgtc cgtgtggaat taaatatgga
    10621 tcaaggtact ttcaaagtta tcgctcgtaa agatgttgtt gaagaagtat ttgacgacag
    10681 agatgaagtg gatttaagta cagcgcttgt taaaaaccct gcatatgaaa ttggtgatat
    10741 atacgaagaa gatgtaacac ctaaagattt tggtcgtgta ggtgctcaag cagcgaaaca
    10801 agcagtaatg caacgtcttc gtgatgctga acgtgaaatt ttatttgaag aatttataga
    10861 caaagaagaa gacatactta ctggaattat tgaccgtgtt gaccatcgtt atgtatatgt
    10921 gaatttaggt cgtattgaag ctgttttatc tgaagcagaa agaagtccta acgaaaaata
    10981 tattcctaac gaacgtatca aagtatatgt taacaaagtg gaacaaacga caaaaggtcc
    11041 tcaaatctat gtttctcgta gccatccagg tttattaaaa cgtttatttg aacaagaagt
    11101 tccagaaatt tacgatggta ctgtaattgt taaatcagta gcacgtgaag ctggcgatcg
    11161 ctctaaaatt agtgtcttct ctgaaaacaa tgatatagat gctgttggtg catgtgttgg
    11221 tgccaaaggc gcgcgtgttg aagctgttgt tgaagagcta ggtggcgaaa aaatcgatat
    11281 cgttcaatgg aatgaagatc caaaagtatt tgtaaaaaat gctttaagcc cttctcaagt
    11341 gttagaagtt attgttgatg aaacaaatca atctacagta gttgttgttc ctgattatca
    11401 attgtcatta gcgattggta aaagaggaca aaacgcacgt ctagctgcta aattaaccgg
    11461 ctggaaaatt gatattaaat cagaaacaga tgcgcgtgaa gcgggtatct atccagtagt
    11521 tgaagctgaa aaagtaactg aagaagatgt tgctttagaa gatgctgaca caacagaatc
    11581 aaccgaagag gtaactgatg tttcagttga aacaaatgta gagaaagaat ctgaataata
    11641 ggttggagtg aagtatctat gaaaaagaaa aaaattccga tgcgaaaatg tattctttca
    11701 aatgaaatgc atcccaaaaa agatatgatt cgtgttgttg ttaataaaga aggcgaaatc
    11761 tttgcggatg ttactggaaa gaaacaaggc cgtggcgcgt atgtttctaa agatgttgct
    11821 atggttgaaa aagcacaaca aaaagaaatt ttagaaaaat attttaaagc atctaaagag
    11881 caattggatc cggtttacaa agaaattatt agattaattt atagagaaga gatcccaaaa
    11941 tgagtataga tcaaatatta aactttttag gattagcaat gagagccggt aaagtaaaaa
    12001 caggtgaatc agtcattgtt aatgaaatta aaaaaggaaa tttgaagctc gttattgttg
    12061 caaatgatgc gtctaataat acagctaaat taattacaga taaatgtaag agttacaaag
    12121 ttccattcag aaagtttgga aatcgaaatg aattgggaat agcacttgga aaaggtgagc
    12181 gtgttaatgt agggattact gacccaggct ttgctaaaaa gttgctatca atgatagatg
    12241 aatatcataa ggagtgatta tatgagtaaa caaagaattt acgaatatgc gaaagaatta
    12301 aatctaaaga gtaaagagat tatagatgag ttaaaaagca tgaatattga ggtttcaaat
    12361 catatgcaag ctttggaaga tgaccaaatt aaagcattag ataaaaagtt caaaaaagaa
    12421 caaaagaacg acaataaaca aagcactcaa aataatcacc aaaaatcaaa caatcaaaat
    12481 caaaataaag ggcaacaaaa agataacaaa aagaatcaac aacaaaataa taaaggcaac
    12541 aaaaagaata atagaaataa taagaaaaat aacaagaata ataaaccaca aagtcaacca
    12601 gctgctccaa aagaaatacc atcaaaagtg acatatcaag aaggtattac agtaggcgaa
    12661 tttgcggata aattaaatgt tgaatcatca gaaattatca aaaaattatt cttacttggt
    12721 attgttgcta atatcaatca atcattaaat caagaaacaa tcgaattaat tgccgatgat
    12781 tatggcgttg aggttgaaga agaagttgtg attaatgaag aagatttatc aatctacttc
    12841 gaagacgaaa aagatgatcc agaagcaatt gagagaccag ctgttgtaac aattatggga
    12901 catgttgacc atggtaaaac tactttatta gattcaattc gtcatacaaa agttacagca
    12961 ggtgaagcag gcggaatcac tcaacatatt ggtgcatatc aaattgaaaa cgatggcaaa
    13021 aaaatcactt tcttagatac accgggacat gctgcattta caacgatgcg tgcgcgtggt
    13081 gcacaagtaa cagatattac aattttagta gtagcagctg acgatggtgt tatgccacaa
    13141 acaattgaag caattaacca tgctaaagaa gcagaagtac caattattgt tgcagtaaat
    13201 aaaattgata aaccaacttc aaatcctgat cgagttatgc aagaattaac tgaatatggt
    13261 ttaattcctg aagattgggg cggcgaaaca attttcgttc cactttctgc attaagtggt
    13321 gatggtatcg acgatttatt agaaatgata ggattagttg cagaagttca agaacttaaa
    13381 gcaaatccta aaaaccgtgc tgttggtaca gttatcgaag ctgaattaga taaatcacga
    13441 ggtccttctg catcattatt agtacaaaac ggtacattaa atgttggtga tgcgattgta
    13501 gtgggtaata cttacggccg tatccgtgca atggttaatg acttaggtca aagaatcaaa
    13561 acggctggtc catcaacgcc tgttgaaatt acaggtatta atgatgtgcc acaagctggg
    13621 gatcgctttg ttgtatttag tgatgaaaaa caagctcgtc gtattggtga atcaagacac
    13681 gaagctagca ttgtacaaca acgtcaagaa agtaaaaatg tttcattaga taacctgttt
    13741 gaacaaatga aacaaggtga aatgaaagac ttaaacgtta ttattaaagg tgatgttcaa
    13801 ggttctgttg aagctttagc tgcatcatta atgaaaattg atgttgaagg cgtaaatgtt
    13861 cgtatcattc atacagcggt tggtgcgatt aacgagtcag acgtgacact tgctaatgcc
    13921 tcaaatggta ttatcattgg tttcaatgtt cgtccagaca gtggtgcaaa acgtgctgca
    13981 gaagctgaaa atgttgatat gcgtttacac agagttattt ataatgttat cgaagaaatt
    14041 gaatcagcga tgaaaggttt acttgatcca gaatttgaag aacaagttat cggacaagct
    14101 gaagttcgtc aaacattcaa agtttctaaa gttggtacta ttgctggatg ttatgttact
    14161 gaaggtaaaa ttacgcgaaa tgctggtgta cgtattattc gtgatggtat tgttcaatat
    14221 gaaggcgaat tagatacact taaacgtttc aaagatgatg ctaaggaagt tgcaaaaggt
    14281 tatgaatgtg gtattacaat tgaaaactac aatgacctta aagaaggcga tgttatcgaa
    14341 gcatttgaaa tggttgaaat taagcgttaa ttaaataaat tacaagctaa aagtttagtt
    14401 aagattgata tgctccctat aaatattaca ctttttaagt gtttacttta tagggagcat
    14461 atttgataca agcttttggt tttttattag aatagattac ctattaaaag ttacgttata
    14521 tagacatgat ttttgtataa aattttgtgg tgacctagaa tgctttttaa tgacaaaata
    14581 taatgtcgac tattattgga aaattttctg ttgaaatgcc tatcttacgg caaactttat
    14641 ttgattttat aggcttaatt tattaaaata acgtgtgagc taaaataatt gtttaagcat
    14701 tgttacacta aaaaatgcaa ataacaattg aacttaaaga taaagaggtg acaagaatga
    14761 gcagtatgag agcagagcgt gttggtgaac aaatgaagaa ggaattaatg gatatcatca
    14821 acaataaagt caaagatcct cgagttggtt ttattacaat tacagatgtt gttttaacaa
    14881 atgatttatc gcaggctaaa gtatttttaa ctgtattagg taacgataaa gaagtagaaa
    14941 atacatttaa agcacttgat aaagcaaaag gcttcattaa gtctgaatta ggttctagaa
    15001 tgcgattacg tattatgccg gaattaatgt atgaatatga tcaatcaatc gaatatggta
    15061 ataaaattga acgaatgatt caagatttac acaaacaaga tagataattt agtgttaggt
    15121 atctggaaaa tgtttgataa tttcttaata tcggtatatt aacattaaac agttaataca
    15181 tagatgtgta gaaatagtta acattttcca gtttttttat gaataaattc agttaatacg
    15241 ctattaaaat atatttttaa aaaagaaggt gacaacatgt ataacggtat attaccagta
    15301 tataaagaac gtggtttaac aagtcatgac gtcgtattca aattgcgtaa aattttaaag
    15361 actaaaaaaa tagggcacac tggtacgctt gatcctgaag ttgcaggcgt gttaccggta
    15421 tgtataggta atgcaacgag agttagtgat tatgttatgg atatgggtaa agcttatgag
    15481 gcaacggtat cgataggcag aagtacaaca actgaagatc aaacgggtga tacattggaa
    15541 atgaagggtg tacattcagc taatttcaac aatgacgata ttgaccgatt gttagaaagt
    15601 tttaaaggta tcattgaaca aattccgcca atgtattcat cagtcaaggt gaatggtaaa
    15661 aaattatatg aatatgcgcg taataatgaa acagttgaaa gaccaaagcg taaagttaat
    15721 attaaagata ttgggcgtat atctgaatta gattttaaag aaaatgagtg tcattttaaa
    15781 atacgtgtca tctgtggcaa aggtacatat attagaacgt tagcgactga tatcggtgtg
    15841 aaattaggct ttccggcaca tatgtcgaaa ttaacgcgaa tcgagtctgg tggatttgtg
    15901 ttgaaagata gccttacatt agaacaaata aaagaacttc atgagcagga ttcattgcaa
    15961 aataaattgt ttcctttaga atatggatta aagggtttgc caagtattaa aattaaagat
    16021 tcgcacataa aaaaacgtat tttaaatggg cagaaattta ataaaaatga atttgataac
    16081 aaaattaaag accaaattgt atttattgat gatgattcag aaaaagtatt agcaatttat
    16141 atggtacacc ctacaaaaga atcagaaatt aaacctaaaa aagtctttaa ttaaaggaga
    16201 tagaatttat gaaagtcata gaagtgacac atcctataca atctaaacag tatattacag
    16261 aggatgttgc aatggcattc ggatttttcg atggcatgca taaaggtcat gacaaagtct
    16321 ttgatatatt aaacgaaata gctgaggcac gcagtttaaa aaaagcggtg atgacatttg
    16381 acccgcatcc gtctgtcgtg ttgaatccta aaagaaaacg aacaacctat ttaacaccac
    16441 tttcagataa aatcgaaaaa attagccaac atgatattga ttattgtata gtggttaatt
    16501 tttcatctag gtttgctaat gtgagcgttg aagattttgt tgaaaattat ataattaaaa
    16561 ataatgtaaa agaagtcatt gctggttttg attttacttt tggtaaattt ggaaaaggta
    16621 atatgactgt tcttcaagaa tatgacgcgt ttaatacgac aattgtgaat aaacaagaaa
    16681 ttgaaaatga aaaaatttct acaacttcta ttcgtcaaga tttaatcaat ggtgagttgc
    16741 aaaaagcgaa tgatgcttta ggctatatat attctattaa aggcactgta gtgcaaggtg
    16801 aaaaaagggg aagaactatt ggcttcccaa cagctaacat tcaacctagt gatgattatt
    16861 tgttacctcg taaaggtgtt tatgccgtta gtattgaaat cggcactgaa aataaattat
    16921 atcgaggggt agctaacata ggtgtaaagc caacatttca tgatcctaac aaagcagaag
    16981 ttgtcatcga agtgaatatc tttgactttg aggataatat ttatggtgaa cgagtgacag
    17041 tgaattggca tcatttctta cgtcctgaga ttaaatttga tggtatcgac ccattagtta
    17101 aacaaatgaa cgatgataaa tcgcgtgcta aatatttatt agcagttgat tttggtgatg
    17161 aagtagctta taatatttag agttgcgtat agttatataa acaatctata ccacaccttt
    17221 ttcttagtag gtcgaatctc caacgcctaa ctcggattaa ggagtattca aacattttaa
    17281 ggaggaaatt gattatggca atttcacaag aacgtaaaaa cgaaatcatt aaagaatacc
    17341 gtgtacacga aactgatact ggttcaccag aagtacaaat cgctgtactt actgcagaaa
    17401 tcaacgcagt aaacgaacac ttacgtacac acaaaaaaga ccaccattca cgtcgtggat
    17461 tattaaaaat ggtaggtcgt cgtagacatt tattaaacta cttacgtagt aaagatattc
    17521 aacgttaccg tgaattaatt aaatcacttg gtatccgtcg ttaatcttaa tataacgtct
    17581 ttgaggttgg ggcatattta tgttccaacc ttaatttata ttaaaaagtt ttttacaa
//
LOCUS       DAKSQE010000054        16910 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.054, whole genome shotgun sequence.
ACCESSION   DAKSQE010000054 DAKSQE010000000
VERSION     DAKSQE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16910
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.054"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(83..1288)
                     /locus_tag="O0V33_001497"
     CDS             complement(83..1288)
                     /locus_tag="O0V33_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCX8976128.1"
                     /translation="MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQ
                     KEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGSKLTTLNNIKIRIR
                     NIKRYSSENLLLNKLDTKYMQIFINKLSDIYSQNQVTRQLGDMKGAIKYAVKFYNYPN
                     EYLLTNVKIPKRRKTIEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIAS
                     ILEVQALTGMRIGELQALQEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKR
                     SISINSRTVEIFKKIILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTK
                     ALNMNKKVTTHTFRHTHITLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDREL
                     TQKLENIPS"
     gene            1414..2028
                     /locus_tag="O0V33_001498"
     CDS             1414..2028
                     /locus_tag="O0V33_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCX8976129.1"
                     /translation="MTQFLGALLLTGVLGYIPYKYLTMIGLVSEKNKVINTPVLLIFS
                     IETCLIWFYSFIIFNNVDLKNLNLIQLLTGLKANILFLFIFVLTVFVFNPLIVKFIIW
                     LINITRKFMKLDCISLLDKRDKLFNNNGKPVFIVIKDFENRIIEEGELKTYNSAGSDF
                     DLLEVERQDFKVSDLPSNDELYIKHTLVDLKQQIKLDLYLMNEY"
     gene            complement(2025..2171)
                     /locus_tag="O0V33_001499"
     CDS             complement(2025..2171)
                     /locus_tag="O0V33_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976130.1"
                     /translation="MNGGENFMVDKNKKQETTRSNPLNKSFEKSGASEKLKSTLSEKA
                     KKKD"
     gene            complement(2208..2390)
                     /locus_tag="O0V33_001500"
     CDS             complement(2208..2390)
                     /locus_tag="O0V33_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976131.1"
                     /translation="MKITNCKIKKETIVYEVLTSGNQPFTYELPKDLSSHNARKYLEF
                     ISQKIDGDKLTKEDSL"
     gene            complement(2460..3230)
                     /locus_tag="O0V33_001501"
     CDS             complement(2460..3230)
                     /locus_tag="O0V33_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCX8976132.1"
                     /translation="MREKVSNRLKHIMKIRNLKQVDIINKSKPYQKQLGISLSKSTLS
                     QYINDVQSPDQDRIYLLSKTLNVGEAWLMGYDVNSYRVPDEERQEETVMSKINNISSQ
                     LTPPRQSNVLNYANSQLDEQNKVTSIDEYKESKLVSYIACGATGAGIGEELYDDILHE
                     EVFFKEDEAPSNADFCILINGDSMEPMLKQGTYAFIKKEDSIKDGTIALVVLDGVSLI
                     KRVDICEDYINLVSLNPKYDDIKVASFSNIKVMGKVVL"
     gene            3390..3611
                     /locus_tag="O0V33_001502"
     CDS             3390..3611
                     /locus_tag="O0V33_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HCX8976133.1"
                     /translation="MCYDYSRLSGKIVEKYGTQYNFAIAMKLSERSLSLKLNGKVGWK
                     DSEIWKAIQLLGIPVEKIHLYFFKEKVHV"
     gene            3635..4081
                     /locus_tag="O0V33_001503"
     CDS             3635..4081
                     /locus_tag="O0V33_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976134.1"
                     /translation="MEQITLTKEELKEIIAKEVREAINGKKPISSGAIFNKVRISHKD
                     FDEINKKFAYTERLRGADNLGLGHPLSLKKYQHGIGCYENYKAYASEIHDHIRKLTLS
                     AFGVTLNSDLKESEYDEASRMYDMLKNFYLYRYQKRIETLSIEDFE"
     gene            4098..4298
                     /locus_tag="O0V33_001504"
     CDS             4098..4298
                     /locus_tag="O0V33_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976135.1"
                     /translation="MQEIKRVVNPQLHSIETGNNLLNKEQKLLKDNLNAEIKNTKLSY
                     AEINEVLYLIDREQHYLANHRR"
     gene            complement(4282..4536)
                     /locus_tag="O0V33_001505"
     CDS             complement(4282..4536)
                     /locus_tag="O0V33_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976136.1"
                     /translation="MKNYYHLLSFDDDLANESANNLLKDGWELVHVGTKLTRILDNGQ
                     AYYNTEYVLGGTKEQYEKYIADSEKADKDLFSQFQLSDDD"
     gene            4596..5372
                     /locus_tag="O0V33_001506"
     CDS             4596..5372
                     /locus_tag="O0V33_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCX8976137.1"
                     /translation="MQALQIIEQNDTHYVDSREVAEMVGKEHKNLIRDVENYRSVILQ
                     SSKLSPDDYFIESTYLGANNRQTKHYLLTKKGCDIVANKMTGSKGILFTATYVDAFHK
                     MDEHIKQQAKLYVPQTPMQALEMMFKAQKDQEQFNQQMQQEITGIRHIVGIETKNWRN
                     DTNKMLSAIAQHLGGGAMHQKVKSEAYKVLEEKGRCNLKIRMQNRKGKMLANGATKTQ
                     INKLSKLDVITDEPRLIEIYISVIKSMAIKYGVDISQFEI"
     gene            5386..5601
                     /locus_tag="O0V33_001507"
     CDS             5386..5601
                     /locus_tag="O0V33_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCX8976138.1"
                     /translation="MEQEQRELLAYIHYILNMEISNTSETYTHTIEEAGKIETIEVSR
                     EQHLEEVMKWAAQEIEKHFDLVPEPED"
     gene            5654..5794
                     /locus_tag="O0V33_001508"
     CDS             5654..5794
                     /locus_tag="O0V33_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976139.1"
                     /translation="MLQKFRIAKERNKLKLKLLKHASYCLERNNNPELLRAVAELLKK
                     VN"
     gene            complement(5809..6441)
                     /locus_tag="O0V33_001509"
     CDS             complement(5809..6441)
                     /locus_tag="O0V33_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976140.1"
                     /translation="MYIDPLKNVRLSINNAINNVEISRSMAIKQSLKLKYQLDIINKN
                     NLNLFSNFKVDFHLNNLIEMNFNLRNSFSSLTFQRNLFSEDAIKSFKELYRFDDEIVL
                     QAQQTIRDFYINPTAISTLAEAINSTYPINEQGTYKRNDKFVNRIKNDFPHPFKQLIR
                     WSNGIAAGADIQIFVTNYINENDLHIQNSLIVAIVCLLSFLSTYCSHSKK"
     gene            6497..6823
                     /locus_tag="O0V33_001510"
     CDS             6497..6823
                     /locus_tag="O0V33_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976141.1"
                     /translation="MNIQKVMKLILKKIHEMREILKKFNKNIRHKDLIVIKVKDENSV
                     PLVIYKGGELKSKRVVKFLWVTRNGNYEGGYDINIEHYAKSEKGRPGRYEKSGFRSLF
                     FKEDSQ"
     gene            6820..7038
                     /locus_tag="O0V33_001511"
     CDS             6820..7038
                     /locus_tag="O0V33_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976142.1"
                     /translation="MNKLYKTTLLITMAVVTWKVWKIEKHTRKPVISSRALSDYLNNK
                     SLTIPKDAENSTESARRLLKFAEQTISK"
     gene            7068..7331
                     /locus_tag="O0V33_001512"
     CDS             7068..7331
                     /locus_tag="O0V33_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCX8976143.1"
                     /translation="MPKIIVPPTPENTYRGEEKFVKKLYATPTQIHQLFGVCRSTVYN
                     WLKYYRKDNLGVENLYIDYSPTGTLINISKLEEYLIRKHKKWY"
     gene            7343..7504
                     /locus_tag="O0V33_001513"
     CDS             7343..7504
                     /locus_tag="O0V33_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HCX8976144.1"
                     /translation="MSNIYKSYLLAVLCFTVLAIVLMPFLYFTTAWSIAGFASIATFI
                     FYKEYFYEE"
     gene            7596..7856
                     /locus_tag="O0V33_001514"
     CDS             7596..7856
                     /locus_tag="O0V33_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCX8976145.1"
                     /translation="MYYEIGEIIRKNIHVNGFDFKLFILKGHMGISIQVKDMNNVPIK
                     HAYVVDENDLDMASDLFNQAIDEWIEENTDEQDRLINLVMRW"
     gene            7866..8087
                     /locus_tag="O0V33_001515"
     CDS             7866..8087
                     /locus_tag="O0V33_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2483 family protein"
                     /protein_id="HCX8976146.1"
                     /translation="MKQTVTYLIKHKDENLFITNRPTEVNDTVKYSTDMRDAREFDGL
                     DKTVIDMSKHKAIKKTVTETIEYEEVEHD"
     gene            8080..8868
                     /locus_tag="O0V33_001516"
     CDS             8080..8868
                     /locus_tag="O0V33_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCX8976147.1"
                     /translation="MTEEKQEPQEKVSILKKLKINNIAEKNKRKFYKFAVYGKIGSGK
                     TTFATRDKDAFVIDINEGGTTVTDEGSDVEIENYQHFVYVVNFLPQILQEMRENGQEI
                     NVVVIETIQKLRDMTLNDVMKNKSKKPTFNDWGEVAERIVSMYRLIGKLQEEYKFHFV
                     ITGHEGINKDKDDEGSTINPTITIEAQEQIKKAITSQSDVLARAMIEEFDDNGEKKAR
                     YILNAEPSNTFETKIRHSPSITINNKKFANPSITDVVEAIRNGN"
     gene            8897..9448
                     /locus_tag="O0V33_001517"
     CDS             8897..9448
                     /locus_tag="O0V33_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCX8976148.1"
                     /translation="MKITGQAQFTKETNQEKFYNGSAGFQAGEFTVKVKNIEFNDREN
                     RYFTIVFENDEGKQYKHNQFVPPYKYDFQEKQLIELVTRLGIKLNLPSLDFDTNDLIG
                     KFCHLVLKWKFNEDEGKYFTDFSFIKPYKKGDDVVNKPIPKTDKQKAEENNGAQQQTS
                     MSQQSNPFESSGQFGYDDQDLAF"
     gene            9461..10132
                     /locus_tag="O0V33_001518"
     CDS             9461..10132
                     /locus_tag="O0V33_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976149.1"
                     /translation="MQYITRYQKDNDGTYSVVATGVELEQSHIDLLENGYPLKAEVEV
                     PDNKKLSIEQRKKIFAMCRDIELHWGEPVESTRKLLQTELEIMKGYEEISLRDCSMKV
                     ARELIELIIAFMFHHQIPMSVETSKLLSEDKALLYWATINRNCVICGKPHADLAHYET
                     VGRGMNRNKMNHYDKHVLALCREHHNQQHAIGVKSFDDKYHLHDSWIKVDERLNKMLK
                     GEKKE"
     gene            10129..10278
                     /locus_tag="O0V33_001519"
     CDS             10129..10278
                     /locus_tag="O0V33_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976150.1"
                     /translation="MNRLRIIKIALLIVILAEEIRSAKKIKKFTPEDSKGFPDITKDS
                     IKEPK"
     gene            complement(10271..10552)
                     /locus_tag="O0V33_001520"
     CDS             complement(10271..10552)
                     /locus_tag="O0V33_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976151.1"
                     /translation="MEKPYMLTYDLNSPGQKYEELRNVIKKEISNGHCNYWKSSFLFR
                     SSLSTSEMIEKLKPYLDSGDKLFVTEIVNNKQGWLTKEQWDFINHNIFI"
     gene            10618..11388
                     /locus_tag="O0V33_001521"
     CDS             10618..11388
                     /locus_tag="O0V33_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HCX8976152.1"
                     /translation="MATFRVYKESGNFVTVHKDFIHDSNISWKAKGILLYLLSRPDNW
                     QIYETELEQHSTDGLSGLKSGIKELEEIGYIQRSRKRDKSGRLNGYEYLVYEQPHHIR
                     FSNVGKTVNGKTNNGKTVNGKSHTTNNNSTNNDLTNNNNTNNEGSILSGNPTVSSIPY
                     KEIIEYLNKKAGKHFKHNTAKTKDFIKARWNQDFRLEDFKKVIDIKTAEWLNTDSDKY
                     LRPETLFGSKFEGYLNQKIQPTGTDQLERMKYDESYWD"
     gene            11398..12177
                     /locus_tag="O0V33_001522"
     CDS             11398..12177
                     /locus_tag="O0V33_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCX8976153.1"
                     /translation="MKPLFSEKINESLKKYQPTHVEKGLKCERCGSEYDLYKFAPTKK
                     HPNGYEYKDGCKCEIYEEYKRNKQRKINNIFNQSNVNPSLRDATVKNYKPQNEKQVHA
                     KQTAIEYVQGFSTKEPKSLILQGSYGTGKSHLAYAIAKAVKSKGHTVAFMHIPMLMDR
                     IKATYNKNAVETTDELVRLLSDIDLLVLDDMGVENTEHTLNKLFSIVDNRVGKNNIFT
                     TNFSDKELNQNMNWQRINSRMKHNARKVRVIGDDFRERDAW"
     gene            12171..12332
                     /locus_tag="O0V33_001523"
     CDS             12171..12332
                     /locus_tag="O0V33_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976154.1"
                     /translation="MVITKQNIKEILHCRDVYAQKMIDFANGDQEKLKKLIDDKLKEK
                     EERPAIVEY"
     gene            12345..12566
                     /locus_tag="O0V33_001524"
     CDS             12345..12566
                     /locus_tag="O0V33_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3269 family protein"
                     /protein_id="HCX8976155.1"
                     /translation="MPKEKYYLYREDGTEDIKVIKYKDNVNEVYSLTGAHFSDEKKIM
                     TDSDLKRFKGAHGLLYEQELGLQATIFDI"
     gene            12576..12980
                     /locus_tag="O0V33_001525"
     CDS             12576..12980
                     /locus_tag="O0V33_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HCX8976156.1"
                     /translation="MSKYNAKKVEYKGIVFDSKVECEYYQYLESNMNGTNYDRIEIQP
                     KFELQPKFGKQRPITYIADFSLWKDGKLVEVLDVKGKATEVANIKAKIFRYQYRDVNL
                     TWICKAPKYTGKTWITYEELIKARRERKREMK"
     gene            12985..13170
                     /locus_tag="O0V33_001526"
     CDS             12985..13170
                     /locus_tag="O0V33_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HCX8976157.1"
                     /translation="MQQQAYINATIDIRIPTEVEYKHFGDVDNEKDALADYLYNNPNE
                     ILEYDNLKIRNVNIEVE"
     gene            13171..13476
                     /locus_tag="O0V33_001527"
     CDS             13171..13476
                     /locus_tag="O0V33_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976158.1"
                     /translation="MARITKETKTVSDGYSREDRETTLNYDYENQEWIAYSSVPTHIT
                     RMTKLYGDDVEVLERLESGTAVLVRAKLPKSAIGFRKLMSEERRQELSERAKRAFGH"
     gene            13604..13963
                     /locus_tag="O0V33_001528"
     CDS             13604..13963
                     /locus_tag="O0V33_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976159.1"
                     /translation="MSIVKINGKPYKFTEHENELIKKNGLTPGMVAKRVRGGWALLEA
                     LHAPYGMRLAEYKEIVLSKIMERESKERKLERQRKKEAELRRKKPHLFNVPQKHSRDP
                     YWFDVTYNQMFKKWQEV"
     gene            13964..14212
                     /locus_tag="O0V33_001529"
     CDS             13964..14212
                     /locus_tag="O0V33_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976160.1"
                     /translation="MPKTDSACKEYLNQFFGSKRYLYQDNEQVAHVHEVNGTYYFHGH
                     YKTMFKGVKKTFDTAEELEIYIKQHDLEYEEQKQPTLF"
     gene            14227..14619
                     /locus_tag="O0V33_001530"
     CDS             14227..14619
                     /locus_tag="O0V33_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976161.1"
                     /translation="MAKIKRKKKMTLLELVEWAWNNPEQVESKVFQSDRMGTLGECSE
                     VHFSTDGHGFYTKVVTDKDIFTVEITEEVTEDTEFDCLVELNDIEGFEIYENDSIREL
                     IDGTSRAFYILNEDKTMTLIWKDGELVV"
     gene            14616..14807
                     /locus_tag="O0V33_001531"
     CDS             14616..14807
                     /locus_tag="O0V33_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976162.1"
                     /translation="MMQTYKVCLCIKFFASKCDYKLKKHYFVKSTNEEKATNMVLKLI
                     RKKLPFETASIEVEKVEAI"
     gene            14807..15238
                     /locus_tag="O0V33_001532"
     CDS             14807..15238
                     /locus_tag="O0V33_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCX8976163.1"
                     /translation="MIQPTREELINFMKKHGAEIVDSITDEQSAIRHFRAQSKVFKDE
                     RDEYKKQRDELIEDIAKLRKRNEELENMWRTVKNELLGRYEHYCFKIRELHPESKANR
                     IGALYIGGKSTADIILSRMEELDGTNEFYEFLGQMEEDTNE"
     gene            15231..15479
                     /locus_tag="O0V33_001533"
     CDS             15231..15479
                     /locus_tag="O0V33_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCX8976164.1"
                     /translation="MNNRKQIEQSVISTSAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIYLDEAVGIMTSQVVYKYEEEQEND"
     gene            15472..16008
                     /locus_tag="O0V33_001534"
     CDS             15472..16008
                     /locus_tag="O0V33_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HCX8976165.1"
                     /translation="MTNTLTIDQLQELLQIQKEFDDRIPTRNSNDTVASMIIEFVEWI
                     NTLEFFKNWKKQPGKPLDTQLDEIADYLAFSLQLTLTIVDEEDLEETTEVMVDLFENE
                     VTLPKLHSVYFVHVMHTLTEQFVKGIDNSIVQVLIMPFLYANTYYSIDQLIDAYKKKM
                     KRNHERQDGTADAGKGYV"
     gene            16045..16281
                     /locus_tag="O0V33_001535"
     CDS             16045..16281
                     /locus_tag="O0V33_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCX8976166.1"
                     /translation="MTQYLVTTFKDSTGLPHEHFTAARDNQTFTVVEAESKEEAKEKY
                     ESQNTPIVYYTNNSKVTLFERPSEEVLGSLFEKK"
     gene            16306..16542
                     /locus_tag="O0V33_001536"
     CDS             16306..16542
                     /locus_tag="O0V33_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976167.1"
                     /translation="MFNTPKMKLPEKHTEVFKTYKNGTPEEKAEIEGCFIKTVKDEDS
                     EFYSPMLASLNEQQLKSMLRQVLFLIDTGDDNDD"
     gene            16535..16684
                     /locus_tag="O0V33_001537"
     CDS             16535..16684
                     /locus_tag="O0V33_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCX8976168.1"
                     /translation="MIKQILRLIFLLAMYELGKYVTEQVYIMMTANDDVEVPSDYVFR
                     AEVSE"
BASE COUNT         6402 a         2454 c         3113 g         4941 t
ORIGIN      
        1 aagaaaagct ttaacgccag tgttctcaag cgttttataa agcttgtaaa aaatataagg
       61 gcaaaaaaag ggcagattta agctaacttg gaatgttttc gagtttttga gttagttctc
      121 tatccatttt ttcagttaca tgagtatata tgcgaatggt tgttttttca tctacatgtc
      181 ctaccctttt cataattgct tttaaagaaa cattcatttc tactaataaa gttatgtgtg
      241 tatgtctaaa tgtgtgcgtg gtaactttct tattcatatt taaagctttt gtagttttct
      301 taagcacacc ggcgatttga ttattacata aaggattccc tttttttgtt gtgaatatga
      361 accctctgtc aacatagctc gaattccatc ttttcaacat tttgttttcc agtattatct
      421 ttttaaaaat ttctacggtt ctagaattga tgctgatact tctttttgaa cttatagtct
      481 ttgtagtgtc tttgtatccg aatccttcct cgtatttaat gcggtgaatt gtacctgtta
      541 tattaatagt tttgtttaat aaatctatat ctttttcctg cagtgcttgt agttctccta
      601 tgcgcatacc agttaaagcc tgtacttcta agatgctggc aattaaaatg cgatttcgct
      661 tgtgtaactt attatcattt agtatatgat cacgtatctg taggacttgg ttcatttcta
      721 aatagttgta cattttagat tcatcttttt cgatatcctc tattgttttt cttcttttag
      781 gaattttgac attagttaac aaatattcat ttggataatt gtaaaattta actgcatatt
      841 taatagctcc tttcatatct ccgagttgac gggttacttg attttgagaa tagatatctg
      901 ataatttatt aataaatatc tgcatatatt ttgtatctag tttgtttaaa agcaagttct
      961 cagagctgta tcgtttaatg tttctaattc ttatttttat attattaaga gtagtcaact
     1021 ttgaacctga tgtttttata tgatattcaa gccattcatc taatagcgcg tgaaaagtca
     1081 aagtttttaa ttcgcttgac gacttgttgt tcagtttttc ttttattttt tcttctaaac
     1141 gaaacattgc ttctttttga gattgttttg tattcttgtt caacacaaca cttacgcgct
     1201 tccatttatc tgtgtatgga tctttgtact tctcgtaata tctgtattta gtttcgttat
     1261 ttttgttttt aaatttttca atccacatgt ttatacctcc tgtaggaacg tacgttctgt
     1321 aaatttgtaa aaaataataa gggtaggtgg gctacccaaa atttagtact aggtactaaa
     1381 tatgttataa taaaataaaa agtaggtgat aagatgactc aatttctagg ggcgcttctt
     1441 cttacaggag ttttaggtta cataccatat aaatatctaa caatgatagg tttagttagt
     1501 gaaaaaaaca aggttatcaa tactcctgta ttattgattt tttctattga aacatgtttg
     1561 atatggtttt atagttttat aatttttaat aatgttgatt taaaaaattt gaatttaatt
     1621 cagttgctta caggtctaaa agcaaatatt ttgtttctat ttatttttgt tttaacagtg
     1681 tttgtattta atcctttaat tgttaaattt attatctggt taattaatat aaccagaaag
     1741 tttatgaaat tggattgtat aagcttatta gacaaaagag acaagttgtt taataacaac
     1801 ggtaaaccag tatttatagt tataaaagac tttgaaaaca gaatcattga agagggtgaa
     1861 cttaaaacct ataattcagc tggtagcgat ttcgatttac tagaagttga gcgacaagat
     1921 ttcaaagtat ctgatttacc gtcaaacgat gaattgtata ttaaacatac gcttgtagac
     1981 cttaaacaac aaattaaatt ggatttatat ttaatgaatg aatactaatc ttttttctta
     2041 gctttttctg ataaagtgct ttttaatttt tcgctggcgc ctgacttttc aaaacttttg
     2101 tttaatgggt tactacgagt agtttcttgt tttttgtttt tatctaccat aaaattctca
     2161 ccaccattca acgtctacac tagtaggcgt ttttttattt agtaaaatca taatgaatct
     2221 tctttggtta acttatctcc atctattttt tgtgaaataa attccaagta tttacgcgca
     2281 ttatgtgacg ataaatcttt aggtaactca taagtgaatg gttgattacc actagttaaa
     2341 acttcatata ctatagtttc tttttttatt ttgcaattag ttattttcat tataaactcc
     2401 ttttaaacac tgctgaaata gacgtctttt atattaaagt gccatatagg cgctattaat
     2461 cacaatacaa ctttgcccat tactttaata ttactaaacg aagcgacttt gatatcatca
     2521 tacttcggat ttagagatac caaattaata tagtcttcgc atatatctac acgcttgata
     2581 agacttactc catctaatac aacgagtgca attgtaccat ctttaataga atcttctttc
     2641 ttaataaaag cgtatgttcc ttgttttaac ataggttcca ttgaatcgcc gttaattaaa
     2701 atgcaaaaat cagcatttga tggcgcttca tcttctttga aaaatacttc ttcgtgtaat
     2761 atatcatcgt acaattcttc tccgatacca gcaccagttg cgccacacgc aatatacgac
     2821 actaacttag attctttata ttcatctata gaagtgactt tattttgttc atctaactga
     2881 ctattcgcgt agttgagtac attgctttgt cttggaggcg tgagctgaga tgatatgtta
     2941 ttaatttttg acatcacagt ttcctcttgg cgttcttcat cgggtacgcg ataagaattt
     3001 acatcatatc ccataagcca agcttcaccg acatttaaag ttttagaaag taggtaaatt
     3061 ctatcttggt caggagattg tacatcgtta atatattgag acaaagtact tttacttaaa
     3121 gatataccta gttgcttttg ataaggtttc gatttattaa tgatatctac ttgttttaaa
     3181 tttcttattt tcatgatgtg ttttagtctg tttgaaactt tttctctcat ttagtgcacc
     3241 tccgtttgat aactttataa taaaccttgt tgaacaaaaa ttcaataaaa aagttcataa
     3301 aacatgaatt tttgtgttga cttaattcaa aacaaggtgt aaagtatagt taagttcacg
     3361 atacatgaac ttcaaaggag gtgtttttta tgtgttacga ctactcgcgt ttgagtggta
     3421 agatagttga aaagtatggc actcagtaca atttcgctat tgctatgaag ttgtccgaga
     3481 gaagcttatc cttaaaactc aacggaaaag ttgggtggaa agatagcgaa atatggaaag
     3541 ctatacaatt gctaggtata ccggtagaaa aaatacattt atattttttt aaagaaaaag
     3601 ttcacgtttg atgaactaga aagtggagga catcatggaa caaatcacat taaccaaaga
     3661 agagttgaag gaaattatag cgaaagaagt tagagaagct ataaatggca agaaaccaat
     3721 cagttcaggt gcaattttca ataaagtaag aatcagtcat aaggattttg atgaaattaa
     3781 taaaaagttt gcttatacag aacgtttaag aggtgctgac aatctcggct taggacatcc
     3841 attatcttta aagaaatatc aacacggaat aggatgttat gaaaattaca aagcatacgc
     3901 tagtgaaatt catgaccaca ttagaaaact tacattatca gcttttggtg taacgcttaa
     3961 ttctgattta aaagagagtg aatacgatga agctagcaga atgtatgaca tgttgaagaa
     4021 cttttattta tatcgttacc aaaaacgaat tgaaaccttg tcaattgaag atttcgaata
     4081 ataaggaggc gcaacaaatg caagaaataa aaagagtagt aaatccgcaa cttcatagca
     4141 ttgaaacagg taataattta cttaacaaag agcaaaagtt attaaaagat aacttaaacg
     4201 cagaaattaa aaatactaaa ctttcatacg ctgaaataaa tgaagttctc tacctaattg
     4261 atagagaaca acattactta gctaatcatc gtcgctaagt tgaaattggc taaataaatc
     4321 cttgtcagct ttttcagaat cagcaatgta tttttcatac tgttccttag ttccaccaag
     4381 aacatattca gtattgtaat aagcttgtcc gttatccaaa atccgagtca attttgtccc
     4441 aacgtgaaca agctcccaac catctttgag aaggttatta gcagattcat tagctaaatc
     4501 atcatcgaaa gacaaaagat gatagtagtt tttcataata ccacctcctt ccattaggag
     4561 ataacaaaat tatacacgaa aggagcataa acattatgca agcattacaa ataatagaac
     4621 agaacgacac acattacgta gatagtagag aagttgcgga aatggtaggc aaggaacata
     4681 aaaatttaat tagagatgtt gaaaattata gaagtgtaat tttgcaaagc tcaaagttga
     4741 gccctgacga ttacttcata gaatcaactt atttaggcgc aaataatcgt cagactaaac
     4801 actacctact aaccaaaaaa ggatgcgaca tagtagcaaa caagatgaca ggcagtaaag
     4861 ggattttgtt cacagcaact tatgttgacg cattccataa aatggatgaa cacattaaac
     4921 aacaagcaaa actttatgta ccacaaacac caatgcaagc attagagatg atgttcaaag
     4981 cgcaaaaaga ccaagaacag tttaaccaac aaatgcaaca agaaatcaca ggtattcgtc
     5041 acattgtcgg tatcgaaaca aaaaactggc gcaatgatac aaacaaaatg ttatctgcga
     5101 ttgcgcaaca tttaggtggc ggagctatgc accaaaaagt taagtctgaa gcttacaaag
     5161 tattagaaga aaaaggacgt tgtaatttaa aaatccgtat gcagaatcgc aaaggcaaaa
     5221 tgctagcgaa tggtgcgacg aagacacaga ttaacaagtt gtcaaaatta gatgtgatta
     5281 cggatgaacc tagattgatt gagatttaca tttcagtgat taagagtatg gcaattaaat
     5341 acggtgtaga tattagccag tttgaaattt aaaggagaat cagagatgga acaagaacaa
     5401 agagaattac tcgcatacat tcattacatt ttgaatatgg agattagcaa tacgtcagaa
     5461 acatatacac acacgatcga agaagcaggc aaaatcgaaa ctatagaagt cagtagagaa
     5521 caacacttag aagaggttat gaaatgggcg gcgcaagaga ttgaaaaaca ctttgattta
     5581 gtgcctgaac cagaggatta ataaccataa tctgccaaca atcgaacaaa catcttaata
     5641 ggaggaacaa caaatgttac aaaaatttag aattgcgaaa gaaagaaata aattaaaact
     5701 caaattacta aagcatgcta gttactgttt agaaagaaac aacaaccctg aactgttgcg
     5761 agcagttgca gagttgttaa agaaggttaa ctaaattaga ccttattatt actttttaga
     5821 atgtgaacaa taggtcgata aaaaacttaa taaacaaact atagcaacta tcaatgaatt
     5881 ttgaatatgt aaatcgttct cgtttatata gtttgttaca aagatttgaa tgtcagcacc
     5941 cgccgcaatg ccattagacc atcttattaa ctgtttgaaa ggatgtggaa aatcattttt
     6001 gatacgattg acaaatttat cgtttctttt ataggtacct tgctcattta ttggataggt
     6061 cgaattgatg gcttcagcca aagtagagat agcagttgga ttgatataaa aatctctaat
     6121 ggtctgttgt gcttgaagca ctatctcatc atcaaaccta tagagttcct taaaagattt
     6181 tatcgcatct tcagaaaata aatttctttg aaatgttaga gaagaaaaag aattacgcaa
     6241 attaaaattc atttcaatta agttgtttag atgaaagtct actttgaagt tagaaaataa
     6301 atttaagtta tttttattaa ttatatctaa ttggtactta agttttaaag actgtttaat
     6361 tgccatgctt ctagaaattt caacattatt aattgcgtta ttaatagata aacgaacatt
     6421 ttttaaagga tcaatataca ccaatatcac ctccttaggt tgataacaac attatacacg
     6481 aaaggagata taacaaatga acattcaaaa agtaatgaaa ttaatattga aaaaaattca
     6541 tgagatgagg gagattttaa aaaagttcaa caaaaatatc agacacaaag atctaattgt
     6601 catcaaagtg aaagatgaaa acagcgttcc attagtcatt tataaaggtg gagagctgaa
     6661 gagcaaacga gtagttaaat ttttatgggt aactagaaac ggaaattacg aaggtggtta
     6721 cgacataaac atagagcatt atgcaaagag tgaaaaaggc agacccggta gatatgaaaa
     6781 atcaggattt agaagtttgt tttttaagga ggattcacag tgaacaaatt gtataaaaca
     6841 accctcctca tcacaatggc agttgtgacg tggaaggttt ggaagattga gaagcacact
     6901 agaaaacctg tgattagtag cagggcgttg agtgactatc taaacaacaa atctttaacc
     6961 ataccgaaag atgctgaaaa ttctactgaa tctgctcgtc gccttttgaa gttcgccgaa
     7021 caaactatta gcaaataaca acattataca cgaaaggaaa gatagaaatg ccaaaaatca
     7081 tagtaccacc aacaccagaa aatacatata gaggcgaaga aaaatttgtg aaaaagttat
     7141 acgcaacacc tacacaaatc catcaattgt ttggagtatg tagaagtaca gtatacaact
     7201 ggttgaaata ttaccgcaaa gataatttag gtgtagaaaa tttatacatt gattattcac
     7261 caacaggcac tctgattaat atttctaaat tggaagagta tttgatcaga aagcataaaa
     7321 aatggtatta ggaggatatt aaatgagcaa catttataaa agctatctat tagcagtatt
     7381 gtgcttcaca gtcttagcaa ttgtactcat gccgtttcta tacttcacta cagcgtggtc
     7441 aattgcggga ttcgcaagta tagcgacatt catattttat aaggaatact tttatgaaga
     7501 ataaaaaaac tgctacttgc gccaacaagt aacagtatca agcacttaag aaaaatttca
     7561 agttaaatat aaaacgaaaa atggaggaag tcaagatgta ttacgaaata ggcgaaatca
     7621 tacgcaaaaa tattcatgtt aacggattcg attttaagct attcatttta aaaggtcata
     7681 tgggcatatc aatacaagtt aaagatatga acaacgtacc aattaaacat gcttatgtcg
     7741 tagatgagaa tgacttagat atggcatcag acttattcaa ccaagcaata gatgaatgga
     7801 ttgaagagaa cacagacgaa caggacagac taattaactt agtcatgaga tggtaggagg
     7861 tcgctatgaa gcagactgta acttatctaa tcaagcataa agatgaaaat ctatttatta
     7921 caaaccgacc aaccgaagtg aacgatacag tgaagtattc aactgatatg cgagacgcaa
     7981 gagaattcga cggactagac aaaactgtta ttgatatgtc taagcacaaa gcaatcaaga
     8041 aaacagtgac agaaacaatt gagtatgagg aggtagaaca tgactgagga aaaacaagaa
     8101 ccacaagaaa aagtaagcat actcaaaaaa ctaaagataa ataatatcgc tgagaaaaat
     8161 aaaaggaaat tctataaatt tgcagtatac ggaaaaattg gctcaggaaa aaccacgttt
     8221 gctacaagag ataaagacgc tttcgtcatt gacattaacg aaggtggaac aacggttact
     8281 gacgaaggat cagacgtaga aatcgagaac tatcaacact ttgtttatgt tgtaaatttt
     8341 ttacctcaaa ttttacagga gatgagagaa aacggacaag aaatcaatgt tgtagttatt
     8401 gaaactattc aaaaacttag agatatgaca ttgaatgatg tgatgaaaaa taagtctaaa
     8461 aaaccaacgt ttaatgattg gggagaagtt gctgaacgaa ttgtcagtat gtacagatta
     8521 ataggaaaac ttcaagaaga atacaaattc cactttgtta ttacaggtca tgaaggtatc
     8581 aacaaagata aagatgatga aggtagcact atcaacccta ctatcactat tgaagcgcaa
     8641 gaacaaatta aaaaagctat tacttctcaa agtgatgtgt tagctagggc aatgattgaa
     8701 gaatttgatg ataacggaga aaagaaagct agatatattc taaacgctga accttctaat
     8761 acgtttgaaa caaagattag acattcacct tcaataacaa ttaacaataa gaaatttgca
     8821 aatcctagca ttacggacgt agtagaagca attagaaatg gaaactaaaa attaattaaa
     8881 aggacggtat ttaattatga aaatcacagg acaagcgcaa tttactaaag aaacaaatca
     8941 agaaaagttt tataacggct cagcagggtt tcaagctgga gaattcacag tgaaagttaa
     9001 aaatattgaa ttcaatgata gagaaaatag atatttcaca atcgtatttg aaaatgatga
     9061 aggcaaacaa tataaacata atcaatttgt accgccgtat aaatatgatt tccaagaaaa
     9121 acaattgatt gaattagtta ctcgattagg tattaagtta aatcttccta gcttagattt
     9181 tgataccaat gatcttattg gtaagttttg tcacttggta ttgaaatgga aattcaatga
     9241 agatgaaggt aagtatttta cggatttttc atttattaaa ccttacaaaa agggcgatga
     9301 tgttgttaac aaacctattc cgaagacaga taagcaaaaa gctgaagaaa ataacggggc
     9361 acaacaacaa acatcaatgt ctcaacaaag caatccattt gaaagcagtg gccaatttgg
     9421 atatgacgac caagatttag cgttttaagg tgtggtttaa atgcaataca ttacaagata
     9481 ccagaaagac aatgacggca cttattccgt cgttgctact ggtgttgaac ttgaacaaag
     9541 tcacattgac ttactagaaa acggatatcc actaaaagca gaagtagagg ttccggataa
     9601 taaaaaacta tctatagaac aacgcaaaaa aatattcgca atgtgtagag atatagaact
     9661 tcactggggc gaaccagtag aatcaactag aaaattatta caaacagaat tggaaattat
     9721 gaaaggttat gaagaaatca gtctgcgtga ctgttcaatg aaagtcgcaa gggagttaat
     9781 agaactgatt atagcgttta tgtttcatca tcaaatacct atgagtgtag aaacgagtaa
     9841 gttgttaagc gaagataaag cgttattata ttgggctaca atcaaccgca actgtgtaat
     9901 atgcggaaag cctcacgcag acctagcaca ttatgaaaca gtcggcagag gcatgaacag
     9961 aaataagatg aatcactatg acaaacatgt attagcgtta tgtcgcgaac atcataacca
    10021 gcaacatgcg attggcgtta agtcgtttga tgataaatat cacttgcatg actcgtggat
    10081 aaaagttgat gagaggctca ataaaatgct gaaaggagag aaaaaggaat gaatagacta
    10141 agaataataa aaatagcact cctaatcgtc atcttggcgg aagagattag aagcgctaaa
    10201 aaaattaaaa aatttacccc tgaggattct aaaggttttc ctgatataac aaaagattca
    10261 ataaaagaac ctaaataaaa atattatggt tgataaaatc ccattgttct tttgttaacc
    10321 acccttgttt gttattgact atttctgtaa caaacagctt atctccagaa tcgagataag
    10381 gtttcaactt ttctatcatt tctgaagttg ataaagaaga acggaataaa aatgaagatt
    10441 tccaataatt gcaatgacca ttagaaattt ccttttttat aacatttctc aattcctcat
    10501 atttttgtcc gggtgagttt aaatcatatg ttaacatata aggtttttcc atattttatt
    10561 cacccccaat ctaacgcagt agcgataaca aaattatacc agaaaggaga taacgaaatg
    10621 gcaacattta gagtttacaa agaatcaggt aactttgtca cagtacacaa agattttata
    10681 catgattcta atataagttg gaaggctaaa ggtattctac tttatttgtt aagtcgacct
    10741 gataactggc aaatttacga aacagaacta gagcaacatt caactgatgg acttagcggt
    10801 ttaaagagtg gaatcaagga actggaagaa attggataca ttcaacgtag tagaaaacgt
    10861 gataaaagtg gtaggttaaa tggttatgag tacttagtat atgagcaacc gcaccacatt
    10921 cgattttcca acgttggaaa aaccgttaac ggtaaaacca acaatggaaa aaccgttaat
    10981 ggtaaatcgc atactactaa taataatagt actaataatg atttaactaa taataacaat
    11041 actaataatg aaggaagtat attgtcgggc aacccgacgg tgtcttccat tccctataaa
    11101 gaaattatcg aatacttaaa taaaaaagca ggaaagcatt ttaaacataa tacagctaaa
    11161 acaaaagatt ttattaaagc aagatggaat caagatttta ggttggagga ttttaaaaag
    11221 gtgattgata tcaaaacagc tgagtggcta aacacggata gcgataaata ccttagacca
    11281 gaaacacttt ttggcagtaa atttgagggg tacctcaatc aaaaaataca accaactggc
    11341 acggatcaat tggaacgcat gaagtacgac gaaagttatt gggattaggg ggatattatg
    11401 aaaccactat tcagcgaaaa gataaacgaa agcttgaaaa aatatcaacc tactcatgtc
    11461 gaaaaaggat tgaaatgtga gagatgtgga agtgaatacg acttatataa gtttgctcct
    11521 actaaaaaac acccgaatgg ttacgagtat aaagacggtt gcaaatgtga aatctatgag
    11581 gaatataagc gaaacaagca acggaagata aacaacatat tcaatcaatc aaacgttaat
    11641 ccgtctttaa gagatgcaac agtcaaaaac tacaagccac aaaatgaaaa acaagtacac
    11701 gctaaacaaa cagcaataga gtatgtacag ggtttctcta caaaagaacc aaaatcatta
    11761 atattgcaag gttcatatgg aactggtaaa agccacctag catacgctat cgcaaaagca
    11821 gtcaaatcta aagggcatac agttgctttt atgcacatac caatgttgat ggatcgtatc
    11881 aaagcgacat acaacaaaaa tgcagttgaa actacagacg agctagtcag attgctaagt
    11941 gatattgatt tacttgtact agatgatatg ggtgtagaaa acacagagca cactttaaat
    12001 aaacttttca gcattgttga taacagagta ggtaaaaaca acatctttac aactaacttt
    12061 agtgataaag aactaaatca aaatatgaac tggcaacgta tcaattcaag aatgaaacac
    12121 aatgcaagaa aagtaagagt aatcggagac gatttcaggg agcgagatgc gtggtaatca
    12181 caaaacaaaa tataaaagaa atattacatt gtagagatgt atatgctcaa aagatgattg
    12241 attttgcaaa cggagaccaa gagaaactta aaaaacttat tgatgataag ttgaaagaaa
    12301 aagaagaaag acccgcaatc gtcgaatatt aaggagtgtt aaaaatgccg aaagaaaaat
    12361 attacttata ccgagaagat ggcacggaag atattaaggt catcaagtat aaagacaacg
    12421 taaatgaagt ttattcgctc acaggagccc atttcagcga cgaaaagaaa attatgactg
    12481 atagtgacct aaaacgattc aagggcgctc acgggcttct atatgagcaa gagctaggtt
    12541 tacaagcaac gatatttgat atttagaggt ggatgatgag taaatacaac gctaagaaag
    12601 ttgagtacaa aggaattgta tttgatagca aagtagagtg cgaatattac caatatttag
    12661 aaagtaatat gaatggcact aactatgatc gtatcgaaat acaaccgaaa ttcgaactac
    12721 aacctaaatt tgggaaacaa agaccgatta cgtatatagc tgatttctct ttgtggaagg
    12781 atggcaaact ggtcgaagtt ttagatgtta aaggtaaggc gactgaagtt gccaacatca
    12841 aagcgaagat attcagatat cagtatagag atgtgaattt aacgtggata tgtaaagcac
    12901 ctaagtacac aggcaaaaca tggattactt acgaggaatt aattaaagca agacgagaac
    12961 gcaaaagaga aatgaagtga tctaatgcaa caacaagcat atataaacgc aacgattgat
    13021 ataagaatac ctacagaagt tgaatataag cattttggtg atgtggataa cgaaaaagat
    13081 gcgctggcag attacttata taacaatcct aacgaaatac tagagtatga caatttaaaa
    13141 attagaaacg taaatataga ggtggaataa atggcaagaa ttaccaaaga aacaaaaact
    13201 gtaagcgacg gttattcaag agaagaccga gaaacgacat tgaactatga ttacgaaaat
    13261 caagaatgga ttgcttactc atcggtaccg acacatatta ctagaatgac aaagttgtac
    13321 ggcgatgatg tagaggtatt ggaacgatta gaatctggga ctgcggtatt ggttagggcg
    13381 aaactaccta aaagcgcaat aggttttaga aaattaatgt ctgaagagcg acgacaagaa
    13441 ttatctgaga gagcaaaaag agcttttggt cattagtgct cgtgaatata gggcgaaaaa
    13501 cgaccaaaaa gacacactaa tactttttag gataaataac atccggagaa aaaaacatga
    13561 gctttaaaaa ttttaacaca ggataaatac agaggtggaa taaatgagta tcgtaaagat
    13621 taacggtaaa ccatataaat ttaccgaaca tgaaaatgaa ttgataaaaa agaatggttt
    13681 aactccagga atggttgcaa aaagagtacg aggtggctgg gcgttgttag aagccttaca
    13741 tgcaccttat ggtatgcgct tagctgagta taaagaaatt gtgttatcca aaatcatgga
    13801 gcgagagagc aaagaacgta aattggaaag acagcgaaag aaagaagctg agctaagaag
    13861 aaagaagcca catttgttta atgtacctca aaaacattca cgtgatccgt actggttcga
    13921 tgtcacttat aaccaaatgt tcaagaagtg gcaggaagta taaatgccta aaaccgatag
    13981 cgcatgtaaa gaatacttaa accaattttt cggatctaag agatatctgt atcaggataa
    14041 cgaacaagtt gcacatgttc acgaagtcaa tggcacttat tactttcacg gacattataa
    14101 aacgatgttt aaaggcgtga aaaagacatt tgatactgct gaagagctcg aaatatatat
    14161 aaagcaacat gatttggaat atgaggaaca gaagcaacca actttatttt agaggagatg
    14221 gaaataatgg caaagattaa aagaaaaaag aagatgacgc tactcgaact ggtggaatgg
    14281 gcatggaaca atcctgaaca agttgaaagt aaagtgtttc aatcagatag aatgggcacg
    14341 cttggagaat gtagcgaagt acatttttca actgatgggc atgggtttta tacaaaagta
    14401 gtaacagata aagatatttt tactgtagaa atcacagagg aagtcactga agatactgag
    14461 tttgattgtc tagtagaact aaacgatatt gaaggttttg aaatatatga aaatgattca
    14521 atcagagagt tgatagacgg tacttccaga gcgttttata tactaaacga agataaaact
    14581 atgacattaa tttggaaaga tggggagttg gtagtatgat gcaaacctat aaagtatgtc
    14641 tttgtatcaa gttctttgca tctaaatgtg attataaatt aaagaaacat tatttcgtga
    14701 aaagtacgaa tgaggaaaaa gccacgaaca tggtattaaa actgattcgt aaaaagctcc
    14761 cgttcgaaac tgcaagcata gaagtcgaaa aagtggaggc aatataatga tacaaccaac
    14821 aagagaagaa ttaattaatt tcatgaaaaa acatggagct gaaattgttg actctatcac
    14881 tgatgagcaa agtgcaataa gacactttag agctcaatca aaagttttta aagacgaacg
    14941 tgatgagtac aagaagcaac gagatgagct tatcgaggat atagctaagt taagaaaacg
    15001 taacgaagag ctggagaaca tgtggcgcac agtcaaaaat gaattgcttg gaagatacga
    15061 acattactgt tttaaaatta gagaactaca ccctgagagc aaagcgaaca ggataggagc
    15121 tctctatata ggaggtaaaa gcactgcaga tattatactg tcgcgaatgg aagaactaga
    15181 cggaacaaat gagttctacg aatttttagg gcaaatggag gaagacacaa atgaataacc
    15241 gtaaacaaat agaacaatca gtgatcagta ctagtgcgta taacggtaat gacacagagg
    15301 ggttactaaa agagattgaa gacgtgtata agaaagcgca agcgtttgat gaaatacttg
    15361 aaggtttacc taatgctatg caagatgcac tcaaagaaga tatttatctt gatgaagcag
    15421 tagggattat gacgagtcaa gttgtctata aatatgagga ggagcaggaa aatgactaac
    15481 acattaacaa ttgatcagtt acaagagtta ttacaaatac aaaaggagtt cgacgataga
    15541 ataccaacta gaaattcaaa tgacacagta gctagtatga ttattgaatt tgtagagtgg
    15601 attaacacac ttgagttttt taaaaattgg aagaaacaac caggtaagcc actagataca
    15661 caattagatg agattgctga ttacttagct ttcagtttgc aattaacttt gactattgtt
    15721 gatgaagaag atttggaaga aactactgag gttatggttg atttgtttga aaatgaagtt
    15781 actttaccta aactacattc agtttatttt gttcatgtaa tgcatacact aacagaacaa
    15841 tttgtaaaag gtattgataa tagtattgta caagttttaa taatgccttt tttgtacgcc
    15901 aatacttact attctatcga ccaactcatt gacgcataca aaaagaaaat gaaaaggaat
    15961 catgaaagac aagatggaac agcagacgca ggaaaaggat acgtgtaaag acatcttaga
    16021 tcgagtcaag gaggttttgg ggaagtgaca caatacctag tcacaacatt caaagattca
    16081 acaggactac cacatgaaca ttttactgct gctagagata atcagacgtt tacagttgtt
    16141 gaggcggaga gtaaagaaga agcgaaagag aaatatgagt cacaaaatac acctattgtt
    16201 tactacacta ataattctaa agtgacctta ttcgaaagac ctagtgaaga agtattaggt
    16261 tctttgttcg aaaagaaata aaatcattaa agaggggaga taataatgtt taatacacct
    16321 aaaatgaaat taccagaaaa gcacaccgag gtatttaaga cgtataaaaa tggaacgcca
    16381 gaagaaaaag ctgagattga aggctgtttt attaaaactg ttaaagatga agatagtgaa
    16441 ttttacagcc ctatgttagc cagtctaaat gaacaacagt taaagagtat gttgagacag
    16501 gtactttttt tgattgatac aggagatgac aatgatgatt aaacaaatat taagactaat
    16561 attcttacta gcaatgtatg agctaggtaa gtatgtaacg gagcaagtat atattatgat
    16621 gacggctaat gatgatgtag aggtgccgag tgattacgtc tttcgagcgg aggtaagtga
    16681 ataatgagaa tatttattta tgatttgatc gttttgctgt ttgctttctt aatatccata
    16741 tatattattg atgatggagt gataataaat gcattaggaa tttttggtat gtataaaatt
    16801 atagattcct tttcagaaaa tattataaag aggtagataa aaatgaacga gcaaataata
    16861 ggaagcatat atactttagc aggaggtgtt gtgctttatt cagttaaaga
//
LOCUS       DAKSQE010000055        16507 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.055, whole genome shotgun sequence.
ACCESSION   DAKSQE010000055 DAKSQE010000000
VERSION     DAKSQE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16507
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.055"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            249..1847
                     /locus_tag="O0V33_001538"
     CDS             249..1847
                     /locus_tag="O0V33_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCX8976169.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTLTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTVSEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAGRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            2228..3706
                     /gene="mqo"
                     /locus_tag="O0V33_001539"
     CDS             2228..3706
                     /gene="mqo"
                     /locus_tag="O0V33_001539"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCX8976170.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKNVLFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFDNLEQRGVEIQYKQNVLDIKKQKSGA
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(3833..4915)
                     /locus_tag="O0V33_001540"
     CDS             complement(3833..4915)
                     /locus_tag="O0V33_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000696075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8976171.1"
                     /translation="MKILAIVRRVLKEMLRDKRTLALMFLAPILILTLMYFIFNGDEN
                     DLKIGIDTSVSSNITKQLPSSDIKTQTFHSHENIKSKIENNHLDGYIYQENQTLYVIY
                     TNEDPNKSNMIKQMIGKAIQQDKISSIKHLIEKAPPTMKEPNKQDIKISHSYLYGNSD
                     STYFDKMFPILMGFFVFLFVFLISGIALLRERTTGTLERVLSTSIRRSEIVLGYLIGY
                     GIFAIIQTLIIVLFSIYLLNINLAGSLWYVILINICLAITALSMGIFISTFANSEFQM
                     IQFIPLVAVPQVFFSGIFPLENMPDWLGNIGYIFPLRYAGDALTNVMIKGQGWSNIWF
                     DLLILMIFIIVFIILNIVGLKRYRQV"
     gene            complement(4912..5640)
                     /locus_tag="O0V33_001541"
     CDS             complement(4912..5640)
                     /locus_tag="O0V33_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8976172.1"
                     /translation="MEATIAKLDKVVKTYNKHHVLNEISMSINKGELLGLIGPSGSGK
                     TTTIKCLLGMEKIDSGSAEIFAHKMPNRKALNRLGYMGQTDALYENLTAHENLEFYGR
                     LSGLKGNILKDNIEKHIVLVNLEQALNKKVAQFSGGMKRRLSLAITLLAEPELIILDE
                     PTVGIDPKLRQQIWQQFKQMTKDGKSVVITTHVMDEAERCDKVGLIVDGRIFAIDTPT
                     NLKQRFNVDTIEAVFIKAEEVCQS"
     gene            5774..6337
                     /locus_tag="O0V33_001542"
     CDS             5774..6337
                     /locus_tag="O0V33_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HCX8976173.1"
                     /translation="MDQTAFTDSRVTKTINRIHDGMVNLLKTKAYNDITVKDICEKSQ
                     ISRTTFYTHYKTKDDFIYSYLNTLLKEAKKKFLKSETLTQAIFFKKMIYFWLSEGQLI
                     LMLLGDESAYKLHQVIKKSLQQRIEINFVPVLNTKMLTTKEKYFLLIFMCNAIIGVLQ
                     DWVKRGYKESPKEVAEIMNKIFENAFR"
     gene            complement(6514..6969)
                     /locus_tag="O0V33_001543"
     CDS             complement(6514..6969)
                     /locus_tag="O0V33_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HCX8976174.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YTHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWGITKATIVHFITTYVGFLPLAI
                     LAGWFPLTINYLIIFTIIFIVVYTLIWIIQFFKNKNYVDTINEQLKQLK"
     gene            complement(6966..7409)
                     /locus_tag="O0V33_001544"
     CDS             complement(6966..7409)
                     /locus_tag="O0V33_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCX8976175.1"
                     /translation="MMKLNLFINANETESYIDIHAPKMNDHVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRFLKSIKERLSI"
     gene            complement(7572..8945)
                     /gene="hssS"
                     /locus_tag="O0V33_001545"
     CDS             complement(7572..8945)
                     /gene="hssS"
                     /locus_tag="O0V33_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HCX8976176.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTIDHKGHKTFYGEPFREDTLSQNA
                     INNVLNNKDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTNDGSIAVFMRPDIGETFS
                     EFRTFLAVLLILLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVDQLIKDIIRHEQFAADEKS
                     LIILADLESIDFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNAYVTTFTI
                     TLPNHSH"
     gene            complement(8938..9612)
                     /gene="hssR"
                     /locus_tag="O0V33_001546"
     CDS             complement(8938..9612)
                     /gene="hssR"
                     /locus_tag="O0V33_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HCX8976177.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAFTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            9748..10803
                     /locus_tag="O0V33_001547"
     CDS             9748..10803
                     /locus_tag="O0V33_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8976178.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQESYKMNIQTLKLSNKEQD
                     VITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYSHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVNEKDLTD
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            10803..11468
                     /locus_tag="O0V33_001548"
     CDS             10803..11468
                     /locus_tag="O0V33_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8976179.1"
                     /translation="MALVVKDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRSSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSKRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(11698..12666)
                     /locus_tag="O0V33_001549"
     CDS             complement(11698..12666)
                     /locus_tag="O0V33_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCX8976180.1"
                     /translation="MLLNLLLIFILIVICPLILNYTLTLNLTCYILVVISIFLSLIYS
                     IMTYTFPWSMFVTFIIISIIMLFKHRYLFSHTSSRLFIKKLLSFSYKFVLYTSALLFI
                     STIQSPIVKGLSMWLAILCLSLLLTFICYLVWTVSYRNRSYNKEVDIIIVLGAGIFTE
                     FVTPMLAARLDRALDIYQQQASATKIIVSGGQGPDEPIPEALAMQRYLIAHGVDKTDI
                     LMESHSINTYTNFLYSKQIINNLYHEAVKIVCVTSQFHVLRALRLAQKLGLSIDGVGS
                     HTPYHFFLHVLIKDYLGVMYQYRLLLTLYCSSSWFICLYAAFIYNS"
     gene            12861..13316
                     /locus_tag="O0V33_001550"
     CDS             12861..13316
                     /locus_tag="O0V33_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCX8976181.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
     gene            13556..14938
                     /locus_tag="O0V33_001551"
     CDS             13556..14938
                     /locus_tag="O0V33_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCX8976182.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGHLKFDFRQSNSETVDVTEDFEEANITVTLKGDTKLNDSSK
                     KVTINDREMAFSSSKTYGPYPQNKDITISASGKAKGKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLVNENGKQVDKEYELEQGPQD
                     RLQLIKTSEK"
     gene            14969..15096
                     /locus_tag="O0V33_001552"
                     /pseudo
     CDS             14969..15096
                     /locus_tag="O0V33_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15206..16414
                     /locus_tag="O0V33_001553"
     CDS             15206..16414
                     /locus_tag="O0V33_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCX8976183.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGIASPLVGVKGEDNPIPYIIIIIATAVILIILQI
                     YNMKVFKTNR"
BASE COUNT         5776 a         2288 c         2896 g         5547 t
ORIGIN      
        1 atagtataag ttacttcatt acattaagta caattcattt tgatgaacag atttaaaatg
       61 aaaaataaaa caattaaata ggaaagtgat attgttaata gtctgaaaaa atggtatatt
      121 ttaattgtga aaaaaatcac attaatctca ctgttctgta aaatgttatc gataacaaaa
      181 tgctatagcc agagcatgat tttaatgaag caattacact actactttat ggaaagaaga
      241 gtcagcctat gttagtaaat acgtttaacc catttgataa tttattatta tcgtctttaa
      301 ttgcagcaat accgattgta ctgtttttac tttgtttaac tgtttttaaa atgaaaggta
      361 tttatgcagc cattacaaca cttgttgtaa cattactaat tgcaatacca tttttcaaat
      421 tgccagttgg tatcgcttct ggtgcagtag tcgaaggttt cttccaaggc atcattccga
      481 ttggctatat cgttatgatg gcggtattgt tatataaaat tactgttgaa tctggacaat
      541 ttttaacaat tcaagatagt attacaaata tttcacaaga ccaacgtatt caagttttac
      601 ttattggatt tgcattcaac gcatttttag aaggtgcagc aggatttggt gtaccaattg
      661 caatttgtgc acttttatta acacaattag gatttaatcc gttaaaagct gcgatgttat
      721 gtttagtcgc aaatgcagcg tctggtgctt ttggtgcgat tggtatccct gtaggtgttg
      781 tagaaacgtt gaaattacct ggagatgttt cagtattagg tgtttctcaa tcagcaactt
      841 taacattggc aatcataaat ttcattattc cattcttact tatctttatt attgatggtt
      901 ttagaggtgt taaagaaaca ttaccagcaa ttttagtagt ttcaatcact tatacactta
      961 cgcaaggatt attaactgtt ttcagcggac ctgaattagc agatattatt ccgccgttat
     1021 taacaatgtt agcattagca gtattttcta aaaaattcca accaaaacac atttatcgtg
     1081 ttaataaaga tgaagaaatt gaacctgcaa aagcacattc tgcaaaagca gtattacatg
     1141 catggagtcc attcattgta ttaacagtca ttgtaatgat ttggagtgcg ccattcttta
     1201 aaaacttatt cttaccaaat ggtgctttat catcattagt atttaaattc aacttacctg
     1261 gaacagtcag cgaagttacg cataaaccat tagtattgac tttaaatatt attggacaaa
     1321 caggtacagc tattttatta actattatta ttacaatttt aatgtctaaa aaggttaact
     1381 ttaaagatgc aggtagatta ttcggtgtta catttaaaga attatggtta ccagttctta
     1441 caatttgttt catcttagca atttctaaaa tcacaactta tggtggttta agtgcggcaa
     1501 tgggtcaagg tattgctaaa gcaggtaatg tcttcccagt tctatcacca atcttaggtt
     1561 ggataggtgt gtttatgaca ggatcagttg taaataacaa ctcattattt gcaccaattc
     1621 aagcttctgt tgcacaacaa attggaacaa gtggttcact tcttgtatct gctaatacag
     1681 ttggtggtgt agcggcaaaa ttgatttcac cacaatctat tgcaattgca actgcagcag
     1741 taaaacaagt tggtaaggaa tcagaattat taaaaatgac attgaaatac agtgtatgtt
     1801 tactaatatt catctgtatt tggactttca tcttgtcatt attataaata aacgtatttc
     1861 aaaatataaa tatacagagg gtgagatgtt ttctaacatc tcatcttttt ttaatggatc
     1921 attgatgaaa gaagtttgac attataataa tggtagcgct ttatgttaaa atgaagagtg
     1981 agtaatcagc aatcaaatta aattggttga taggtgttaa ggtttgtggt tttgtctttg
     2041 tgctatcgca cataaagtat ataattaaag tagtttcgtt attataaaat attaatatac
     2101 atagtagata gtaatagagc atcaccatga gaacctgttg agacacttat tgattcaaag
     2161 tggtattaat atgtcgtatt tctcgaacgt tccattattc attttaaaaa gggggactgt
     2221 atttgttatg acaacacaac atagcaaaac agatgtcatc ttaattggtg gcggtattat
     2281 gagtgcaaca ttaggaacat tacttaaaga attatcacct gagaaaaata ttaaagtgtt
     2341 tgaaaaatta gcacaacctg gcgaagagag ttcaaatgta tggaataatg ccggtacagg
     2401 gcattcagca ctttgcgagt tgaactatac aaaagaaggt aaggatggca cagttgattg
     2461 tagtaaagca attaagataa atgagcagta ccaaatttca aagcagtttt gggcatattt
     2521 agttaaaaca ggacaattag ataacccaga tcgctttatt caagcggtgc cacacatgag
     2581 ttttgtcatt ggcgaagata acgtagcttt tataaaaagt cgtgttgcaa cgttaaagaa
     2641 aaatgtttta ttcgaaaaaa tgaaattatc gcaagatgaa gaagaaatga aatcttgggt
     2701 accgttaatg attgaagggc gtaagtctga tgaaccaatt gctttaactt atgatgaaac
     2761 tggtacagat gttaactttg gtgcgttaac tgcaaagtta tttgataatt tagaacaacg
     2821 tggtgtggaa attcaatata agcaaaatgt attagacatc aagaaacaga aatctggagc
     2881 atggctagtt aaagttaaag atttagaaac taacgaaacg acaacatatg aatctgattt
     2941 tgtatttatt ggtgctggcg gtgcgagttt accattactc caaaagactg gaattaaaca
     3001 atcaaaacat attggtggtt tcccggtaag tggattattc ctgcgctgta caaatcaaga
     3061 agtgattgat cgtcatcatg ctaaagtgta cggaaaagca gcagtgggtg cgccaccaat
     3121 gtcagtgcca cacttagata cacgttttgt agacggcaag cgttcattgt tatttggtcc
     3181 atttgcaggt ttctcaccta aatttttaaa aacaggttca catatggatt taattaaatc
     3241 ggttaaacca aataatatcg tgacgatgtt atctgcaggt atcaaagaaa tgagtcttac
     3301 gaagtattta gtgtcacaat tgatgttatc taatgatgag cgtatggatg atttaagagt
     3361 ctttttccca aatgctaaaa atgaagattg ggaagtgatt acagcagggc aacgtgtcca
     3421 agtaatcaag gatacggagg attctaaagg taacttacaa tttggtaccg aagtcattac
     3481 gtcagatgat ggcacattag ctgcattact tggtgcatca cctggtgcgt caacagctgt
     3541 agatattatg tttgatgttt tacagcgatg ctatcgtgat gaattcaaag gatgggaacc
     3601 aaagattaaa gaaatggtgc cgtcatttgg ttatcgctta acagatcatg aggatttata
     3661 tcacaaaatt aatgaagaag taactaagta tttacaagtt aaataataaa cgaaacggta
     3721 atgtcttttt aaatgtgata gacattaccg ttttttagtg gttcttttta ttatcagtgc
     3781 tttatattta gaagcttatc ttgattgaag tcatttaaaa gttttggaga atttatacct
     3841 gtctgtatcg ttttaaacca acaatattta agataataaa gactatgata aagatcatta
     3901 gaattagtaa atcgaaccat atattactcc aaccttgccc cttaatcata acattggtta
     3961 aagcatcacc agcatatctg agagggaaga tatagccaat gtttcctagc caatcaggca
     4021 tattttcaag tggaaaaatt cctgaaaaga atacctgagg tacagcaact agtggaataa
     4081 attgaatcat ttgaaactca gaattagcaa acgttgaaat gaaaattccc atagataatg
     4141 ctgtaatagc taaacaaata ttaatcaaaa ttacatacca taagctacct gctaaattaa
     4201 tgttcaataa atatattgaa aataggacta tgattaatgt ttgaatgatt gcgaatatac
     4261 catagccaat cagataacca agaacgattt cacttcgacg aattgaagtt gataatacac
     4321 gttctaacgt acctgttgta cgttcacgta ataaagctat acctgatatt agaaatacaa
     4381 ataaaaatac aaagaagccc attagtattg gaaacatttt atcgaaataa gtactatcag
     4441 aattaccgta taaataggaa tgtgaaatct taatatcttg tttgtttggt tctttcatgg
     4501 tcggaggtgc tttttcaatt agatgcttta tactgcttat tttatcttgt tgtatagctt
     4561 tgccaatcat ttgtttgatc atattagatt tgttagggtc ttcatttgta taaatgacat
     4621 ataatgtttg attttcttgg taaatataac catcaagatg attattctcg attttagatt
     4681 tgatattttc atgagaatgg aacgtttgag tttttatgtc ggacgatggc aattgttttg
     4741 ttatatttga tgaaacagat gtatcgatac cgatttttaa atcgttttca tcaccgttaa
     4801 aaataaaata cataagcgtc aatattaaaa ttggtgctag aaacattagc gcgagtgtac
     4861 gtttatcacg taacatttct tttaatactc gtctaacaat agctaaaatt ttcatgattg
     4921 acaaacctcc tcagctttaa taaataccgc ttcaattgta tctacattaa atctttgttt
     4981 taaattggtt ggcgtatcta tagcaaatat tcttccgtcg acaataagtc caactttatc
     5041 acaacgttcc gcctcatcca taacatgtgt tgtgatgacg acactctttc cgtctttggt
     5101 catttgcttg aactgttgcc atatttgttg gcgcaattta gggtcaatgc ctacagttgg
     5161 ttcatctaat atgattaatt ccggttcggc caacaatgtt attgctaaag ataatcggcg
     5221 tttcatgcct cctgaaaact gtgcaacttt tttatttaac gcttgctcta aattcactag
     5281 aacgatatgt ttttcaatat tatcttttaa tatatttcct ttcagacctg acaaacgacc
     5341 gtaaaactct aggttttcat gagcagttaa gttttcgtat aatgcatcag tttgccccat
     5401 atagcctaat cgatttaaag ctttgcgatt aggcatctta tgagcgaaaa tttctgcaga
     5461 tccagaatct atcttttcca tacctagtaa acactttatt gttgttgttt taccagaacc
     5521 actaggacct attaacccta gcagttcacc tttattgatt gacatactaa tttcgtttaa
     5581 tacgtgatgc ttgttatacg tttttacaac tttatccaac ttcgcgatag ttgcttccat
     5641 gataacccct ccaaaattga gatacatttc actttattta tatatcatca taagcgtata
     5701 ttaagattgc gaataaaata ttaatagata gtgaacatat ttgtttttag tgttcagaaa
     5761 aaaggtgggg catatggatc aaacagcttt tacggatagt cgtgtaacga aaacgataaa
     5821 tcgcatacat gatggaatgg taaatctatt aaaaacgaag gcatataatg atataactgt
     5881 taaagatata tgtgaaaaga gtcaaataag tcggacaacc ttttatacac attacaaaac
     5941 taaagatgat tttatataca gctatcttaa tacattgtta aaagaagcaa aaaagaaatt
     6001 tcttaaaagt gaaactctga cacaagcaat attttttaaa aagatgattt atttttggct
     6061 aagcgaaggt cagctcattt tgatgttatt aggtgatgag agtgcttata aattacatca
     6121 agtcataaaa aaatcactac aacaacgtat cgaaattaat tttgtaccag tattaaatac
     6181 aaaaatgtta acgacgaagg aaaagtattt tttacttatt tttatgtgta atgcaatcat
     6241 tggtgtgtta caagattggg tcaaacgagg atataaagag agtcctaaag aagtagcaga
     6301 aattatgaat aaaatatttg aaaatgcatt tcgataggtt tttatcatga tgagttgatg
     6361 tcgtaaagga cagttgcagt tacaatttaa tttgaaaata gtaaaaatat attattcagt
     6421 tcatgcgtat ttaaaataaa tacgacataa agctatacat tgcagaagag actactttaa
     6481 tgcaatatat agcttttata attttaataa aaatcatttt aattgtttca gttgctcgtt
     6541 aatagtgtct acgtagttct tgtttttaaa gaattgaata atccaaatca atgtgtaaac
     6601 cacaataaag ataattgtaa atatgattag gtaatttatt gttagtggaa accaaccggc
     6661 aagtattgct aaaggtaaga atccgacata cgttgttatg aaatgcacta tagttgcttt
     6721 agtaatgccc caatctgtgt atttaaagat aaaatctcca aggaaaaaga cgacgcctat
     6781 gagtaaccat aaaatgatag aaatcaacat tacggtagtt tctgtgaaat gcgtataata
     6841 caatatgcca atagttgatt gtgggttcag tggataatat ttgccgtctg caaataacat
     6901 actaaagaac agtgaaaggg ataaaccaat gattaagcta ataaataatg agtttttcaa
     6961 atttttcata ttgataagcg ctcctttata gattttaaaa aacgtctaga agaataggtg
     7021 tagtgtgcat ctttaagata catacgtata agtccatttg gctctaataa taatttttca
     7081 atgtaatact tgttgacgat ttctgatttg gaaatgcgaa tgaaatgttg tggtaattgt
     7141 ttttctagtt cataaagtcg taattttagt ttgaactttt gattgcttgt aatggctgtt
     7201 acatttttat taataacttg gaatgttata acatctgata cattaatgat atggatttct
     7261 ttgtctatgt atccgactaa tgtatgtgat ttgtctaaat cattgactgc attaataata
     7321 ctttgaacgt gatcattcat tttaggtgca tgtatatcaa tataagattc cgtctcattt
     7381 gcattgataa ataaattgag tttcatcata ggttaatgcc tccttcaaaa ttattaaacc
     7441 ataaacgacc attgatatat ttaaaatgtg ttgaatggta gaaattaaat gttaagtggc
     7501 tagaaagcgc taatcaatat aaaagatacc tcctgaaata aaaacagaaa tgttctttca
     7561 ggaggtagag attaatgtga atgatttggc agtgtaatag taaaggtggt tacatacgcg
     7621 ttactttgtg tgaattggat tgtaccatga tgcaattcaa tgatggattt tgtaattgcg
     7681 agacctaaac cattactatt atcatgtttg ctcactttat agaaacgttc aaataaacgt
     7741 gcttcagctt gtggactaat tggtgaacca tcattactta ttgtgaaaat gatgttgttg
     7801 tgactatgtt gcaaagcgat gtcaatggca ccaccaacat ctgtatactt aatagcattt
     7861 attaataaat tactcaatgc ttgatgtaac aaacgttgat ttcctaggaa atcgattgat
     7921 tctaggtcag ctaaaatgat taacgacttt tcatcagcag caaattgttc atgtcgaatg
     7981 atatctttaa tgagttgatc gacttgaatc ttatcatcga ataataaatg ttggtgatta
     8041 tctaattcgg ataagagcaa caattcagtt gttaatccgc tcagctgcgt tgtaatagta
     8101 taaatatcat ttatatattg ttgtcgtaat gttttatcag aagtttgttg taactcgctt
     8161 aataaatgat gaatatgtgt taatggtgtt ttgatttcat gagatacatt ttgtacaaaa
     8221 tgctgcctca tttgatcaac ttgtcccaat gactctctca tcttattaaa gtgatactgt
     8281 aatgttccaa tttcatcttt gcgtgtttgt ttgataggtg tttcaaaatc accatcaatt
     8341 aagcgttcgg tcgctagctt taactttttt actggacgaa tgattgaata ggttgatgcg
     8401 ataactaatg aaattgagat aaataataac aatattaaca agacggctaa gaaagttcta
     8461 aactcgctaa atgtttctcc aatatctgga cgcataaata ctgctattga accgtcgttt
     8521 gtcttaaagt taataccaac agtattatct gttacattgt cgaaaaatcc agttacgaat
     8581 aatgcgaatg gtttatcttt aataccatgg taatctttat tatttaacac gttgttaata
     8641 gcattttgtg ataaagtatc ttctctaaaa ggttccccat aaaatgtctt atgacctttg
     8701 tgatcaatgg tcattatttg gtaattcatt tgccctaaat gtttgaaata ttgttgaatg
     8761 tgcgttggtt tagctgattg ttcatattgt cttgcttctt taagcgtctt catgattttc
     8821 gcgtcattag atgcttttaa attataatga tagtaaacat ttgttaatac aaaacttatt
     8881 aatgcactaa ataaaataac cgtaatggaa taaatcgcaa ttctagcata gagtgtttta
     8941 aacatgattc tccaccttat agccttgtcc tcttactgtt tcaattgtaa gtgtggcatt
     9001 taattttttt aatctttggc gtagtcgctt aatatgaacg tcaactgttc gttcatctcc
     9061 ttcataatca tagccccaaa ttttttctat tatctgttcg cgagtaaaga tttgcttagg
     9121 acgtgctgca agcataaata ataattgaaa ttccttgttc ggtaacgtca tcgttttatt
     9181 agatacttgg agttccaaat aggattggtt tagcgttaag ttgccaatgg tcatttctga
     9241 atttgaattg atattatatc gacgtaatac cgcacgaatt ctaaaaataa gttccttaac
     9301 ctcaaagggc ttggttacat aatcgtcagt accacttata aacgcacgct ctttgtcact
     9361 aagtgcatcc cgtgctgtta acataataac tggtatatca taatcatttt ttaatgtatt
     9421 acataattga aagccgtcca taccatccat cataatatct accactgcaa tatcgacacg
     9481 ctgtttttct aaaagtttta atgcagcttc accacttggt tgtgtgaatg catcaatgtg
     9541 ctctgtttgt aaatggctag ctatataatt taggattcga ggatcgtcat cgacaacaag
     9601 acattgcacc ataactataa actcccttat ctttttcatt tattatacat gtaaaatatt
     9661 tttgcgtaaa aaaacaattg ttcatattga gttcatattt caaccttata ctgacgctaa
     9721 agaagaaata gggagaagtg aatcgatatg aaattagcga taaaagagat tatgttttac
     9781 aaatttcgtt atattttaat cacattaatc attcttttat taagtattat ggtgttattt
     9841 attagtggtt tagctcaagg gcttggtagg gagaatattt cgttatttga acactttgat
     9901 aatgatgaat atgttgttca aaaaatgaaa gagccgcaaa ttgagaaatc gcaacttagt
     9961 gatacgcaac agaatcaaat taaaaaggtt attcatcaag aatcatataa aatgaatatt
    10021 caaacattga aattatctaa taaagaacaa gatgtgatta caatgaatga cgttaaacaa
    10081 caacgcatac aacttaaaaa aggtgattat cctaaaaatg ctcatgaggt cgctattaat
    10141 gataagttag ctgcagacaa cattagagtc ggagatagat tacattttaa aaataattca
    10201 actagttata gagtttctgg tattttaaac gacacaatgt attcgcatag ttccattgtg
    10261 ctattgaacg ataacggatt taatgcattg aataaggtta atacggcatt ttatccagtg
    10321 aaaaatttaa cgcaacaaca acgtgatgag cttaataaaa taaatgacgt tcaagttgtg
    10381 aatgaaaaag atttaacaga taatattgcg agttatcaag cagagcaagc accgttaaat
    10441 atgatgattg tgagtttgtt tgctattaca gcaattgttc taagtgcatt tttctatgtt
    10501 atgacgattc aaaaaatatc acaaattggc attttgaaag caattggtat taagacaaga
    10561 catttattga gtgcgttagt tttacaaatt ttaacactaa caataattgg ggtaggtatt
    10621 gctgtgatca tcatagtagg actatcattt atgatgcctg taacgatgcc tttttattta
    10681 acaacgcaaa atattttatt gatggtgggg atatttatat tagtagcgat tttaggtgcc
    10741 tcactatcat ttatcaaatt atttaaggtg gatcctatcg aagcaattgg aggtgcagaa
    10801 taatggcatt agttgttaaa gatatcgtca aaaatttcgg agaaggtttg tctgaaacaa
    10861 aagttttaaa aggtattaat tttgaagtgg aacaagggga atttgtcatt ttaaatggtg
    10921 cctctggttc tgggaaaaca acattgctaa cgatattagg cggattgtta agtcaaacga
    10981 gtggtacagt gctttacaat gatgcaccat tgtttgataa acagcatcgt tctagtgatt
    11041 tacgattgga agatattggt tttatttttc aatcttcaca tttagttcct tatttaaaag
    11101 tgatagagca attgacactc gtaggccaag aagcgggaat gaccaaacaa caaagttcaa
    11161 aaagagcaat acaacttttg aaaaatattg gtttagaaga tcgcttgaat gtatatccgc
    11221 atcagttatc tggcggtgaa aagcaacgtg ttgcgattat gagagcattt atgaataatc
    11281 cgaaaatcat tttagcagat gagcctacag caagtttaga tgccgataga gcaacaaaag
    11341 ttgttgagat gatacgtcaa caaattaaag aacaacaaat gattggtatt atgattacac
    11401 acgatcgaag attatttgaa tatgcagatc gagtgattga attagaagat ggcaaaataa
    11461 ctgattagtg gcttgtaaag acgctaaatg ttaataattt aagacatagt agtataaaag
    11521 ttagataaca gaatacgatt tgggtttaca aaaagcacgt cactattata taaaatgatg
    11581 acgtgcttta gtttatttat tttcaatata acaattgtct aatcaaggat taacgaatat
    11641 ttaaagatag tttgacgcaa tattagaaac aacctataat aatagtttat ttgtgggtta
    11701 actattataa ataaaagcag cgtatagaca tataaaccaa ctacttgaac aatataacgt
    11761 taataacaat ctatactgat acattacgcc tagataatct ttaatgagca catgtaagaa
    11821 aaagtgatat ggtgtatggc ttccgacacc atcgatagat aaacctaatt tttgggctag
    11881 tcgtaaggcg cgtaatacat gaaactgact tgttacacaa acaattttaa ctgcttcatg
    11941 atacaaattg ttgatgattt gtttagaata taaaaagttt gtgtatgtat ttatagagtg
    12001 agattccatt agtatatctg ttttatcaac accatgtgca atcaaataac gttgcatagc
    12061 taaagcttca ggaattggtt cgtctggtcc ttgtccgcca gatacaatga tctttgttgc
    12121 tgatgcttgt tgttgatata tatcaagtgc acggtctaaa cgcgctgcaa gcattggtgt
    12181 gacaaattcg gtaaaaatac cagcacctaa cacaattatg atatcaactt ctttgttgta
    12241 tgatctattt ctatatgata ctgtccaaac gagataacaa ataaaggtta gtaacaggga
    12301 aagacataat atagctaacc acatagacaa acctttcaca ataggtgact gaatcgtact
    12361 tataaataga agtgctgatg tgtagagtac aaatttatat gaaaaagata ataatttttt
    12421 aataaataag cgactagaag tatgagaaaa taaatatcta tgtttgaata gcatgataat
    12481 actgattatt ataaatgtaa caaacataga ccaagggaaa gtataggtca tgatgctata
    12541 gatgagtgac aaaaatatcg atatgacaac taagatgtag catgttaaat ttaacgtcag
    12601 agtatagttg agaattaacg gacaaataac gataagtata aatattaata ataaattcaa
    12661 taacatactg acacctcgct tataataaat attaagtata aatgtagatg atttaattta
    12721 ttaaagcaag gagaacgtag caacatgtaa gtcttaattt gttatatttt atatgggtca
    12781 atatttttgt gttttttaga tgtgcgatac aatgattgct gaggatactt tataaaaata
    12841 gtaattaggg agaaataatt atggtaaaac atttacaaga ccatattcaa tttttagagc
    12901 agtttataaa taacgttaac gcattaactg caaaaatgtt gaaagattta caaaatgaat
    12961 atgaaatttc attagagcag tctaacgtat taggtatgtt aaataaagaa cctttgacaa
    13021 ttagtgaaat cacgcaaaga caaggtgtaa ataaggccgc agtaagccga cgaattaaaa
    13081 agttaatcga tgctaaatta gttaagttag ataaaccaaa tttaaatatt gatcaacgtt
    13141 tgaaattcat aaccttaact gacaaaggta gagcatattt gaaagaacgt aatgcgatta
    13201 tgacagatat tgcgcaagat attactaatg atttaaattc tgaagatatt gaaaatgtaa
    13261 ggcaagtatt agaagtcatc aatcatcgca ttaaaacata tagcaatcat aaataggttt
    13321 ttgaaggtgt tcaaatataa gactaaatat gtatgtattg aacaccttct ttttagttat
    13381 aattgagaga gtactttcat aaataatgta ataaggtaca attctaaagt aaagttaatc
    13441 atttcttgaa ttgtattctt gggataatat ttgtaaaata ataatggtaa aataataaaa
    13501 taatttttat gatatacata taacaattga agaggaacag aattgaggtg aaagtatgaa
    13561 atcttgcccg aagtgcggac aacaagcaca agatgatgta cagatatgta cacaatgtgg
    13621 acataaattt gatagtcgtc aagcattata tagaaagtca accgatgaag atatacaaac
    13681 taataacatc aagatgagaa aaatggtgcc ttgggcaatt ggttttttca ttttaatctt
    13741 aattattata ttgttctttt tactaagaaa cttcaattca cctgaagcgc aaactaaaat
    13801 attagtgaat gcgattgaga ataatgataa acaaaaagta gcaacattat taagtactaa
    13861 agataataaa gtagattctg aagaagcgaa agtatacatt aactatatca aagatgaagt
    13921 tgggcttaag caatttgtca gcgaccttaa aaatacggta cataaattga ataagagtaa
    13981 gaccagtgta gcttcttata ttcaaaccag atctggtcaa aatatattac gtgtaagtaa
    14041 aaatggcacg cgttatatct ttttcgataa tatgagcttt actgcaccta ccaagcaacc
    14101 aattgttaaa ccgaaagaaa aaacaaaata tgagtttaaa tctggtggta agaaaaagat
    14161 ggttatagct gaagcaaata aagtgacgcc aataggtaat tttataccgg ggacatatag
    14221 aattccagct atgaaatcaa ctgagaacgg tgattttgca ggccatttaa aatttgattt
    14281 tagacaaagt aattctgaaa cggtagatgt tactgaagat tttgaagaag caaatataac
    14341 tgtaacttta aaaggcgata caaaattaaa tgatagttct aaaaaagtaa ctataaatga
    14401 ccgtgaaatg gcattttcaa gttccaaaac gtatggtcca tatccacaaa ataaagatat
    14461 taccatttca gcttcaggta aagcgaaagg taaaacattt acaacacaga cgaagacgat
    14521 taaagccagc gatttaaaat acaatacaga gataactttg aattttgaca gtgaagatat
    14581 cgaagactat gttgaaaaga aagaaaaaga agaaaacagc ttgaagaaca aattgataga
    14641 attctttgct ggatattctt tagcgaataa tgctgcgttt aatcagtcgg attttgattt
    14701 tgtatcgtca tatataaaaa aaggatcatc tttttatgat gatgtaaaga agcgtgtatc
    14761 taaaggaagt ttaatgatga ttagttcacc acaaattata gatgccgaaa aacatggtga
    14821 taagattact gcaaccgtaa gattagtaaa tgaaaacggt aagcaagtag ataaagaata
    14881 tgagcttgaa caaggtccgc aagaccgctt gcaattaatc aagacatcag aaaaatagag
    14941 tataaaactt aaataagatg catgtgtcat ggaatacaaa caacatcctt tcaaagtatt
    15001 tacaattagt gtgaattact ttgaagggat tgttttttgt gaaaaaatta tggagtgctg
    15061 aggtttatat cattttatac cttttataag ttgtaatgat gttcatgaaa cttgtgagta
    15121 atgctaatat ataaaagagc atatttaaaa ttagtttctg ttcgtgatag ttataaacta
    15181 atcataatat aaaggagata gtcatatgaa tgatttaacg cattttaaca agcgttcacc
    15241 gatatttatt attattttag gatctttgac tgccattgga gcattatcaa tagatatgtt
    15301 tttgcctgga ttaccagata ttagacatga ttttcaaaca acgacgtcta acgcacaatt
    15361 aacattatcg atgtttatga ttggtttagc gttcggtaat ttgtttgctg gtccaatttc
    15421 agattcaact gggcgcagaa agccgcttat tatagctatg attattttta cattagcaag
    15481 tttaggtatt gtttttgtac ataatatttg gctgatggtt gcattaagat ttcttcaagg
    15541 agtgacaggt ggagcagcag cagttatttc aagagctata gcaagtgata tgtatagtgg
    15601 taatgaacta actaagttta tggcattatt gatgcttgtt aatggtattg caccagttgt
    15661 tgcgccaaca atagggggca taattttaaa ttattctgtc tggcgtatgg tctttgtcat
    15721 tctgactatc tttggcttcg tcatggtcat aggttcttta ttaaaagtac ctgaatcatt
    15781 aacagtgaca aatcgtgagt caagtagtgg attaaagaca atgtttaaaa acttcaaaat
    15841 attattgaag acgccacgtt ttgtattacc aatgctcatt caaggtatga catttgtaat
    15901 attatttact tatatttctg cgtctccgtt tattattcaa aaaatttatg gcatgactgc
    15961 aatacaattt agttggatgt ttgcaggaat tggtattacg ctcattatat caagtcaact
    16021 tactgggtac cttgttgatt ttatagactc acaaaaattg atgagaggca tgacgatgat
    16081 tcagattatc ggtgtaattt tagtgacgat tgtattgctt aatcattgga acttttggat
    16141 tttagcgata ggatttatca ttttaatagc accagtgaca ggtgttgcga cacttggttt
    16201 tacaatagct atggatgaaa gtagtagtgg tagagggagt tcatcaagtt tattaggttt
    16261 agtgcagttt ttatttggtg gtattgcatc gccattagtg ggggtaaaag gtgaagataa
    16321 tcctatacct tacataatta ttattatagc tacagcagtt atattaatta ttttacagat
    16381 atataatatg aaggtattta aaacaaatcg atgataaaca tagtttcttc tattataatg
    16441 aattgaaaaa tttaaaaaca aaacttgagc tattcataga cctatgaatt tagcatcaaa
    16501 aataaaa
//
LOCUS       DAKSQE010000056        16493 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.056, whole genome shotgun sequence.
ACCESSION   DAKSQE010000056 DAKSQE010000000
VERSION     DAKSQE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16493
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.056"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            153..353
                     /locus_tag="O0V33_001554"
     CDS             153..353
                     /locus_tag="O0V33_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCX8976184.1"
                     /translation="MWITMTIVFAILLLVCISINSDRAREIQALRYMNDYLLDEVVKT
                     KGYNGLEEYRIELKRMNNDIKK"
     gene            376..837
                     /locus_tag="O0V33_001555"
     CDS             376..837
                     /locus_tag="O0V33_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976185.1"
                     /translation="MYSRKEIREMIDNYKWMKNIIDSKVYDNESTSIAQYGYQSAMPK
                     AKGTTSNKVLVKVINKNKALRKYDYLIKKIAFIDEYEEYITNEKDYHILQMLKQRESH
                     NRIMSILDIGRDNFYSRVKDIVNILYNLQQETDTSYTSDSSDTSYKSYTSD"
     gene            952..1404
                     /locus_tag="O0V33_001556"
     CDS             952..1404
                     /locus_tag="O0V33_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HCX8976186.1"
                     /translation="MEISKYQEIATRTHNDELNLNESITCYGLGLSQSTGNVTDLIKQ
                     HMFCNVPIDKGIMINELSESLWNIANLANVLGINLDAIAGHSVNAIMMNKPNQSIDVD
                     NGIKQGDKVLLHGSEYYVDGVIGNLLLISNDEDDRQVNMQDVKKVNKE"
     gene            1411..1776
                     /locus_tag="O0V33_001557"
     CDS             1411..1776
                     /locus_tag="O0V33_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCX8976187.1"
                     /translation="MSIMKRCSHPTCNTLINHNESYCDKHKRYVNDNYNDVRRRNDPE
                     YVRFYNSSQWKRLRGIVLMENDYICRLCGRQAQMVDHIIPTKVDWSKRLEKENLQPLC
                     FECHNKKTKKERREVPRIK"
     gene            1870..2340
                     /locus_tag="O0V33_001558"
     CDS             1870..2340
                     /locus_tag="O0V33_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCX8976188.1"
                     /translation="MAGRPKKLLSNSNKNYTKEEIIEKERQEAQLNKFSKIDTEPPHF
                     LDEVAKQEYLRIVPHMQELPISNLDKAQLAQYCSFYSDFVKASLILEREDLMLEDDRG
                     NKKVNPAFNVKEKAGIRMQQTANTLGLTIDSRLRIMVPEEKEDDDPYMKFASDD"
     gene            2340..4034
                     /locus_tag="O0V33_001559"
     CDS             2340..4034
                     /locus_tag="O0V33_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase family protein"
                     /protein_id="HCX8976189.1"
                     /translation="MIDYVTQYAKKVVSGEILASKKNIQVCKRHLSFIKNPPNGCYWD
                     NRLANKAIEFVEMLPDPKTNEPMPLMEFQKFIVGSLYGWRRGQYRMFTKAYISMARKQ
                     GKSLIVSGMSVNELLFGQYPKFNRQIYVASSTYKQAQTIFKMASQQVNLMRSKSKLIR
                     EKTDVRKTDIEDVLSSSVFAPLSNNPDAVDGKDPTVAILDELASMPDDEMYSRFKTGM
                     TLQKNPLTLLVSTAGDNLNSQMYQEYKYIRRILEGKVKADNYFVYCAEMDSQDEVQDE
                     AKWIKAMPLLESKEHRKTILQNIKSDIQDELEKGTSYHKILIKNFNLWQAQREDSLLD
                     ITDWEQAVTNTPDIKGKDVYIGVDLSRLDDLTSVGFIFPTDNKSVFLDSHSFIGLRTN
                     LEQKIKRDKINYNLVIEKGEAETTRSESGMIDYKQVIEFIIDFIETHDLNVKAVCYDP
                     WNAQSFVTTIESMHLDWPLIEVGQSFKALSQSIKEFRMWVADKRIQHSDNTLLTTAVN
                     NAILVRDGEDNVKINKKINRQKIDPIISIITAFTEARMHEFQEDWTKIYESEEFGF"
     gene            4048..4251
                     /locus_tag="O0V33_001560"
     CDS             4048..4251
                     /locus_tag="O0V33_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976190.1"
                     /translation="MNLNKITDVFHLLVANLVSILFLLGLCIVNISIYKTFSQNMGLL
                     ATGIILIVISLILNHESNQERRH"
     gene            4254..5510
                     /locus_tag="O0V33_001561"
     CDS             4254..5510
                     /locus_tag="O0V33_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCX8976191.1"
                     /translation="MGIFYKTEMRDLKYNEEDLQMMVQTLPGFQGTKLREYEGIEAIK
                     HSDIFTAVMMIASDLARMPIRLISNGQIDYGNKIVNLLNNRPNPMYNGYIFKLVVFAS
                     ALLTSHGYVEIARDKIGKPTNLTFRKTSEVELKSDRRGQPYYFHQRVDDNGRRIQRNI
                     KFSDMLDVKFYSLDGINGLSLLDTLSRTIESDNNGKDFLNNFLRNGTHAGGILKMKGV
                     LENKTARNRAREEFHKAFSGTKQAGKVVVLDESMTFDQLEVDTEVLKLIRENKSSTRE
                     IAGVFGIPLHKFGIETSNMSITDANLDYLSTLKPYITCVCAELNFKFNNEHEDVNREF
                     KFDTTEIRVVDEKTQAEIDKINIGSGKMNIDEIRQRDGLAPIPDGYGSIHRVDLNHVN
                     IALVDEYQMNKSKRIDNKLKGDDENG"
     gene            5929..6513
                     /locus_tag="O0V33_001562"
     CDS             5929..6513
                     /locus_tag="O0V33_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCX8976192.1"
                     /translation="MSKETRVGNIIEVRSNDDNEMVIEGYALKFDTWSENLGGFKETI
                     SRRALENTDLSDVRCLVDHIPSQIIGRTKSGTLQLETDDVGLKYRCKLPNTTFARDLY
                     ENMRVGNINQCSFGFMLDDKGDEMRFDEQENIYKRTLTAIRKLTDVSVVTYPAYKDTD
                     VKPALRSIESIKKEQRKKELELRLKKHSILNKIW"
     gene            6600..7835
                     /locus_tag="O0V33_001563"
     CDS             6600..7835
                     /locus_tag="O0V33_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCX8976193.1"
                     /translation="MKTKEELRSEISDIKRQIDLKVKYATRALNNDELERAEELEQEI
                     TDLRSQIQEKQEELDKLKEKDGGSEDDPQPVVVNEARSYQQQANINELGISIQNTKVT
                     SQEVRDFSEYLETRDENTIKGGSLKTDSGFVLIPEEIVTDILTLKEVEFNLDKYVTVK
                     KAPSGSGKYPVVRQSSVAALPEVEELAENPELAVKPFYQLVYDIKTHRGYFRISRESI
                     EDSKVNVLQELKLWMARTIAATRNQAIIDVLKNGSQGEGGKQLKLEKVAAKGIDGLKD
                     AVNLNIKPNYEHNIAIVSQTMFAKLDKMKDKNGNYLIQPDVKEKTQQRLLGAKVEILP
                     DEMLGEKANETLIFGNLKDAIVLFDRSQYQAGWTDYMHFGECLMVAVRQDCRILDEKS
                     AIVINYEDTKNVGDVSLEA"
     gene            7872..8030
                     /locus_tag="O0V33_001564"
     CDS             7872..8030
                     /locus_tag="O0V33_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976194.1"
                     /translation="MAMFKVKKSYTDLEKGEYLESGKHVEMTVKRADYVNKKLKEHGV
                     ILERVKEE"
     gene            8039..8371
                     /locus_tag="O0V33_001565"
     CDS             8039..8371
                     /locus_tag="O0V33_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCX8976195.1"
                     /translation="MQLTTTELKLLKMHCKIDHNSEDKLLETYYSWAFYEIVSAVTDD
                     YIEYEDWFKSNPLFTRAVYPLANYYFENRIAYQDRNLSLAPHMVLSTVHKLRDSFERY
                     LESEEDEI"
     gene            8361..8693
                     /locus_tag="O0V33_001566"
     CDS             8361..8693
                     /locus_tag="O0V33_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HCX8976196.1"
                     /translation="MKFNSNKLTERVDFCQDISERVNGNPAKPKSKVLYSCYACIREA
                     KESDTQTNLNTGSKFIKTIIIRDPRGDYKPSNKHYITHESQRYNIKYVKSDYQDKSYL
                     RVYGEVVI"
     gene            8693..9070
                     /locus_tag="O0V33_001567"
     CDS             8693..9070
                     /locus_tag="O0V33_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCX8976197.1"
                     /translation="MGAKIEENTIEQGLKNAVLKMNLNGNAIIKAGAMSLVPLLKSNT
                     PFADTKKHAREHIGVSNVKTDRDSSEKIVTVGYTKGVSHRIHATEFGTMYQSPQLFIT
                     KTEKQGKDKVLKTMIATAKRLQK"
     gene            9067..9447
                     /locus_tag="O0V33_001568"
     CDS             9067..9447
                     /locus_tag="O0V33_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976198.1"
                     /translation="MINITNLIRNTIIKENVTEESHVFNYTVDDHFHEKTNKPIVRIY
                     PLPFNPDEYADDSEFTREYNYQIDIWWSEDEPNEQAEKIVESLKKANFQSYYREPLYE
                     SDVMSFRHIIRAKGSILSMKLEEN"
     gene            9448..10401
                     /locus_tag="O0V33_001569"
     CDS             9448..10401
                     /locus_tag="O0V33_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCX8976199.1"
                     /translation="MIEKLKQAPRFLKLNLQHFADTGVSGIAIGVSNFYYAPILKDTE
                     KEWETGAGTRIRFLKEIEVDRPQDTEEDYGDDMVAATAVSNGKLSVKTTFVTVPADDK
                     AFLNGAKKGTGGYKYGAKDIPPDVAIVFERRNHDESSEWVGLFKGKFTRSSIKGQTKQ
                     DKVEFQNDDVEGNFIDRLFDESSHVTGYDAKGSTTGRDYVFMETFGKTYDEFMSSRGE
                     QTTESVEKEMKKTEKVEVKSVNISDEQVTVKVDETKQLSATTEPSGQKVTYAVTEGQT
                     YASVSSSGLVKGLAEGSATVTATSGKKTDTVQVTVQSNLEM"
     gene            10466..10912
                     /locus_tag="O0V33_001570"
     CDS             10466..10912
                     /locus_tag="O0V33_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976200.1"
                     /translation="MERTSIELITGFTKTGKPQYQKYLAKPIITLFETIQGSKLGLKL
                     NKAFKGSDFKELTEEEFNNLSVTEQEEYKNKQEEIEDNMALQMEVLEEVLDFIVEAFD
                     NQFTSIELQKGLPNGQEGIEKIGQLIGRITGGEPSDTKKFVTENQK"
     gene            10972..11094
                     /locus_tag="O0V33_001571"
     CDS             10972..11094
                     /locus_tag="O0V33_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976201.1"
                     /translation="MIEKGMDAEKVANMPIHFFLDIVESKIETKRTAKSFKDIF"
     gene            11150..15799
                     /locus_tag="O0V33_001572"
     CDS             11150..15799
                     /locus_tag="O0V33_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCX8976202.1"
                     /translation="MPNPIGNMVIKVDLDGSGFNRGVTGLNRQMKMVSRELSANLSQF
                     SRYDNSLEKSKIKVEGLSKKQKVQAQITKELKDSYDKLSKETGENSAKTQAAAAKYNE
                     AYAKLNQYERELNQATQELKDMQREQKALNTAMGKLGTNFNNFGPKLQEIGNSMKNVG
                     RNMTMYVTAPVVAGFAVAAKKGIEFDDSMRKVKATSGATGEEFEALKKKAREMGATTK
                     FSASDSAEALNYMALAGWDSKQMMEGLSGVMDLAAASGEELGAVSDIVTDGLTAFGLK
                     AKDSGHFSDVLAQTSSKANTDVRGLGEAFKYVAPVAGALGYTIEDTSIAIGLMSNAGI
                     KGEKAGTALRTMFTNLSSPTRAMGNEMERLGISITDSNGKMIPMRKLLDQLREKFKHL
                     SKDQQASSAATIFGKEAMSGALAIINASDEDYQKLTRSIDSSTGASKRMADTMESGLG
                     GKLRTLRSQLEELALTIYDRIEPALKIIVSAFSKVVTWVTKLPTSIQLAVVGFGLFAA
                     VLGPLVFMFGLFISVMGNAMTVLGPLLINVNKAGGIFAFLRTKIASLVKLFPILGMSI
                     SSLTLPITLIVGALVGIGIAFYQAYKRSETFRNIVNQAISGVANAFKAAKLALQGFFD
                     LFKGDSKGAVTLEKIFPPETVAGIQNVVNTIRTTFFKVVDEIVGFAKEIGAQLASFWK
                     ENGSEITQALQNIAGFIKATFEFIFNFIIKPIMFAIWQVMQFIWPAVKALIVSTWENI
                     KGVIQGAINIILGIIKVFSSLFTGNWRGVWDGIVMILKGTVQLIWNLIQLWFVGKILG
                     VVRYFGGLLKGLITIIWVAIIGVFKKSLSAIWNATKSIFGFLFNSVKSIFTNMKNWLS
                     STWNNIKSNTVGKAHSLFTGVRSKFTSLWNATKDIFTKLRNWMSNIWNSIKDNTVGIA
                     GRLWDRVRNIFGSMRDGLKSIIGKIKDHIGGMVDAVKRGLNKLIEGLNWVGGKLGMDK
                     IPKLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGRGNGPNGFRNEMIEFPNGKRVLT
                     PNTDTTAYLPKGSIVYNGAQTYSMLNGTLPRFSIGTMWKDIKSGASSAFNWTKDQIGK
                     GTKWLGDKVGDVMDFIDNPGKLLNYVLQAFGVDFSSLTKGMGIAGDITKAAWSKIKKS
                     AIKWLEDAFAESGDGGVLDMSKLRYLYGHTAAYTRETGRPFHEGLDFDYIYEPVPSTI
                     NGRAQVMPFHNGGYGKWVKIVKGALEVIYAHLSKYKVKTGQQVRVGQTVGISGNTGFS
                     TGPHLHYEMRWNGRHRDPLPWLRKNNGGGKSTPGGNGAANARRAIKAAQNILGGRYKA
                     SWITNEMMRVASRESNYTANAVNNWDSNARAGIPSRGMFQMIDPSFRAYAKSGYNNPL
                     NPTHQAISAMRYIVGKWVPRTGSWRAAFKRAGDYAYATGGKVYNGLYHLGEEGYPEWI
                     IPTDPSRANEAHKLLALAANDIDNRSKNKRPNNLPNPSISNSDTNYIHTLENKLDAVI
                     NCLVSLVESNQVIADKDYEPVINKYVFEDEVNNSIDKRERHESTRVRFRRGGTII"
     gene            15799..>16493
                     /locus_tag="O0V33_001573"
     CDS             15799..>16493
                     /locus_tag="O0V33_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCX8976203.1"
                     /translation="MQDTIQIDNKTIGWLVVQRGFEIPSFNFVTEKENVKGRAGSIVK
                     NRYLNDIEFDLPLIIRNEKLSPGGEKTHDDILEALVKFFNIKDLTPKKLKFKSQNWYW
                     FAYFDGPLKLPKNPRGSVKFTIKVVLTDPYKYSVTGNKNTAISDQVSVVNSGTADTPL
                     IVEARAIKPSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHSEFRDFKGWTKMITE
                     DIPSNDLGGKVGG"
BASE COUNT         6028 a         2336 c         3412 g         4717 t
ORIGIN      
        1 agttttatca cttcagagta ccgacgaaaa ataaacgatt ataatcttta tcttgataaa
       61 tttgaagaac agtttagtca aaagtttaaa ataaacagaa cttcgataaa agaaagaatt
      121 attattaatt taaacaagag gagatttaaa tgatgtggat tactatgact attgtatttg
      181 ctatattgct attagtttgt atcagtatta atagtgatcg tgcaagagag atacaagcac
      241 ttagatatat gaatgattat ctacttgatg aagtagttaa aactaaaggg tacaacgggt
      301 tagaagaata caggattgaa ttgaagcgaa tgaataacga tattaaaaag taatttatat
      361 tatcggaggt attgcatgta tagcaggaaa gaaatacgtg aaatgataga taactacaaa
      421 tggatgaaga acataataga cagtaaagtc tacgataacg aaagtacatc aattgcacaa
      481 tatggttatc aatctgcgat gccaaaagca aaaggcacga ctagcaataa agttttagtg
      541 aaagttataa acaaaaacaa agcgcttaga aagtacgatt acttgattaa gaagatagcg
      601 ttcattgatg aatatgaaga atacatcacg aatgaaaaag attatcatat tttacaaatg
      661 ttaaaacaac gagaaagcca taataggatc atgagcattc ttgatatagg cagagacaat
      721 ttttattcta gagtaaaaga tatagtaaat atactttata acttgcaaca agaaaccgac
      781 acatcgtaca catcggacag ttcggacaca tcgtacaaat cgtacacatc ggactaattt
      841 tgatatgaca tattattttt tattataata gctgcgtagc aaaacattta tatttatttt
      901 gaactctcac attaagtgag ggtttttatt tttataaaca agaggtggag aatggagata
      961 tcaaagtatc aagagatagc tacacgtaca cacaatgatg aattgaattt aaatgaatct
     1021 attacttgtt acggtctagg attatctcaa tctacaggta atgtcacaga tctaattaaa
     1081 caacatatgt tttgtaatgt gccgatagat aaaggaatta tgataaacga acttagcgaa
     1141 tcgttatgga atatagctaa cctagctaac gtgctaggta ttaacttaga tgcgatagct
     1201 ggtcatagtg ttaacgctat tatgatgaac aaacctaatc aatctatcga tgtggacaat
     1261 ggtataaagc aaggcgacaa agtattgtta catggtagtg agtattatgt cgatggagta
     1321 ataggaaact tgttattaat tagcaatgat gaagacgata gacaggtgaa tatgcaagat
     1381 gttaagaaag tgaacaagga gtgatgtaca ttgtctatca tgaaacgatg tagtcatcca
     1441 acatgtaaca cattgattaa tcataatgaa agttattgtg ataaacacaa acgatatgta
     1501 aatgataatt acaatgatgt gagacgtaga aacgatccag agtatgtaag attttataac
     1561 tcaagtcaat ggaagagatt gcgtggaatc gtactgatgg aaaatgatta tatttgtaga
     1621 ttgtgtggac gacaagcgca aatggttgac catattattc caacaaaagt tgattggtca
     1681 aaacggttgg aaaaggaaaa cttgcagcct ttgtgctttg aatgtcacaa caaaaagacg
     1741 aaaaaggaac gaagggaagt cccccgcatc aaataacggg ggtggtaata atagcctcgc
     1801 gaaacgatgc ccatatatac taacgaagaa ttccctttat ttaagttttt taataggagg
     1861 tgctaattta tggcgggtag accgaagaag cttttgtcaa attcgaataa gaattataca
     1921 aaagaagaaa ttattgaaaa agagcgtcaa gaagctcaat taaataaatt ttctaaaatt
     1981 gatactgaac caccacactt tttagatgaa gtagcgaaac aagaatacct aagaattgta
     2041 ccgcacatgc aagaattgcc aatttcaaac ctagataaag cgcaattagc acaatattgt
     2101 agcttttata gcgattttgt taaagcaagt ttaattttag aacgtgaaga cttgatgtta
     2161 gaagacgaca gaggaaacaa aaaggtcaat ccagcgttca acgttaaaga aaaagcgggt
     2221 attcgaatgc aacaaacagc taatacttta ggtttaacta tcgatagtcg attgcgtatt
     2281 atggttcctg aagaaaaaga agacgatgac ccgtatatga aatttgcgag tgatgactaa
     2341 tgatagatta tgttactcaa tatgcaaaaa aggtggtttc aggtgaaata ttagcaagta
     2401 agaaaaacat acaagtgtgt aaacgtcacc tttcttttat aaagaatccg ccgaatggtt
     2461 gttattggga caatcgtttg gctaataaag cgattgaatt tgtcgaaatg ctaccagatc
     2521 ccaaaacaaa cgaacctatg ccactgatgg aatttcaaaa gtttattgtt ggtagtttgt
     2581 acggctggcg tagaggtcag tacaggatgt ttactaaagc ttacataagt atggctagaa
     2641 aacaaggtaa atctttgatt gtatctggta tgtctgtaaa cgaattactt ttcggacaat
     2701 accctaagtt caacagacag atttatgtag cttcatcaac ttataaacaa gcacaaacaa
     2761 tattcaagat ggcaagtcaa caagtaaacc taatgcgtag taaaagtaag cttatccgtg
     2821 aaaaaacaga cgtaagaaag acggacattg aagatgtatt aagtagttcg gtgtttgcgc
     2881 ctctgtccaa taacccagat gcggttgatg gtaaagaccc tacagttgct attttagatg
     2941 aattagcaag tatgccagac gatgaaatgt attcaaggtt taaaacgggt atgacattgc
     3001 aaaagaaccc tttgacattg cttgtttcaa cagctgggga taatttgaac agtcaaatgt
     3061 atcaagagta caaatatatt agacgtattt tagaaggtaa agttaaagct gataattatt
     3121 ttgtatattg tgctgaaatg gattcgcaag acgaggttca agatgaagcg aagtggataa
     3181 aagccatgcc acttttggaa tcgaaagagc atagaaaaac aattctacaa aatattaagt
     3241 ctgatataca agatgaatta gaaaaaggta cgtcgtatca caaaatatta attaaaaatt
     3301 ttaatctgtg gcaagcacaa agagaagata gtcttcttga cataacagat tgggaacaag
     3361 cagtaacaaa tacacctgat atcaaaggta aagatgttta tataggagtc gatttatcaa
     3421 ggctggacga cttaacctct gtaggtttta ttttccctac tgataataaa agtgtgtttt
     3481 tagatagtca ttcttttata ggtttaagaa ctaatttaga acagaagatt aaaagagata
     3541 aaataaatta taatttagtg attgaaaaag gcgaagcaga aacaactcgc tcagaaagcg
     3601 gaatgataga ttataaacaa gttatagaat tcattattga ttttatagaa acgcatgatt
     3661 taaacgtaaa agccgtctgt tatgacccgt ggaatgctca aagttttgtt acaaccattg
     3721 aatctatgca tttagattgg ccgcttattg aagtgggaca gagttttaaa gcgttatcgc
     3781 aatcgatcaa agaatttaga atgtgggttg cagacaaaag aatacaacat agtgataata
     3841 cattacttac aacagcagtc aataacgcta ttttggttcg agatggagag gataatgtaa
     3901 aaatcaataa gaaaattaat agacagaaaa tagaccctat tatttcgata ataactgctt
     3961 ttactgaagc aagaatgcat gagttccaag aagattggac aaaaatatac gaaagtgaag
     4021 agtttgggtt ttaaaggtgg tgacagtatg aatttaaata aaataactga tgtctttcat
     4081 ttattggttg ctaatttagt tagcatcctt tttttattag gattatgtat tgtgaatatc
     4141 tctatatata aaacttttag tcagaacatg ggattgctag caactggcat tattttaata
     4201 gttatttcat tgatattaaa ccatgaaagc aatcaagaaa ggaggcattg atcttgggta
     4261 ttttttataa aactgaaatg cgtgatttaa aatacaacga agaagatttg caaatgatgg
     4321 tacaaacgtt acctggcttt caaggaacaa aactacgcga atatgagggt atagaagcta
     4381 ttaaacatag cgatatattt actgcagtta tgatgattgc atctgattta gcacgtatgc
     4441 caattaggtt aatatcgaat ggacaaattg attatggcaa taagattgta aacctattga
     4501 acaatcgtcc taatccgatg tataacggtt atattttcaa gttagttgta tttgccagcg
     4561 cattattaac ttcacatgga tatgtcgaga ttgctcgcga taaaatcggt aaaccaacaa
     4621 atttaacgtt taggaaaacc tcagaagttg aattgaagtc agacagaaga gggcaacctt
     4681 attattttca ccaaagagta gatgataacg gtagaagaat tcaacgtaat attaaattca
     4741 gtgacatgct agatgttaaa ttttattcct tagacggtat aaacggttta tcactactag
     4801 atactttaag taggactatt gaatctgata ataacggcaa ggatttcctc aataatttct
     4861 tacgtaacgg tacgcatgcg ggcggtatat tgaagatgaa aggcgttctg gaaaataaga
     4921 ctgcaagaaa tagagcaaga gaggagtttc acaaagcttt tagtgggaca aaacaagctg
     4981 gtaaagtcgt tgttttagat gaatctatga catttgacca attagaggtt gatacagaag
     5041 ttttaaagct tattagagaa aataaatcat ctacaagaga gatagcaggt gtgtttggca
     5101 ttcctttgca taaattcggt atagaaactt cgaatatgag tatcactgac gcaaaccttg
     5161 attatctctc aacgttaaaa ccttatatca cttgtgtttg tgcggaattg aatttcaagt
     5221 ttaataacga acacgaagat gtgaaccgtg aatttaaatt tgacaccact gaaatacgtg
     5281 tggttgatga aaaaacgcaa gctgaaatcg ataaaatcaa tattggttca ggaaaaatga
     5341 atattgatga aataagacaa agagatggct tggcaccgat tccagatggt tacggaagta
     5401 ttcacagggt tgatttgaat catgtgaata ttgcgctggt ggatgaatat caaatgaata
     5461 aatcaaaacg cattgataat aagctgaaag gtgatgatga aaatggctaa attaattgtg
     5521 atcaaaggtc cgccttgtgc tggcaaatca acaatggttc aaaagagatt atcagacaag
     5581 gatgtagtgt ttgattggga tttggtgcaa cgtgctatta ctcatttaga tattcatgat
     5641 cataatgaaa atgctaaaca tataatcgct ggtttcagaa aaatatttat aaatgattct
     5701 caaacaaata aagattttga aaatttttac cttttgacgt gcaatatgac tgactcgtta
     5761 aatcgtcaac ttgaaaattg tgactatgat attgaagagg ttgaagcgac ggaagaggaa
     5821 tgtttgaatc gccttgaaaa agatgacagc aggcctaata aagaaatatt caaacaacgc
     5881 atacatgatt attttgagca acattcttct aaagaggagg tgaggaaaat gagtaaggaa
     5941 acgagagttg gtaacattat tgaagtacgt tcaaacgatg ataatgaaat ggtcatcgag
     6001 ggatatgcct taaagttcga cacttggtct gaaaatcttg gtggctttaa agaaacgatt
     6061 tcgcgtcgcg ctttagaaaa cactgattta tctgatgtac gttgtttagt agatcatatc
     6121 ccgtcgcaaa taatcggtag gacaaagtca ggtactttgc aactcgaaac tgacgatgtc
     6181 ggacttaaat atcgttgtaa gttgccaaat acaacatttg cgcgtgattt atacgagaac
     6241 atgcgcgtag gtaacattaa tcaatgttcg tttggtttca tgcttgacga caaaggcgat
     6301 gaaatgcgtt ttgatgaaca agaaaacatc tacaaacgca ctttgacagc aattcgtaaa
     6361 cttacagatg tctctgtagt aacttatcca gcgtataaag atacggatgt taaacctgca
     6421 ttacgtagta ttgaaagtat taaaaaagag caacgtaaaa aagaattaga attaagacta
     6481 aagaaacatt caatattaaa taagatttgg tgaagttgaa caccattatc aaatacaacc
     6541 attggacatg ctgaatatag cgatgtctat tttttatgcc aattttagga ggaaattaaa
     6601 tgaaaacaaa agaagagtta cgatctgaga tttcagacat caaaagacaa attgatttga
     6661 aagttaagta tgcaacgcga gcgcttaata acgatgagtt agaaagagca gaagaattag
     6721 aacaagaaat tactgattta cgttctcaaa ttcaagaaaa gcaagaagaa ttagataaat
     6781 taaaagaaaa agacgggggt tcagaggatg acccacaacc agttgttgta aacgaagcgc
     6841 gttcttatca acaacaagcg aatataaacg aattaggtat ttcgattcaa aatacaaaag
     6901 taacatcaca agaagttaga gacttttcag aataccttga aactcgtgat gaaaatacta
     6961 ttaaaggcgg ttctttgaaa acggattctg gtttcgtatt aattccggaa gagattgtga
     7021 cagatatcct tacgttaaaa gaagtcgaat ttaatttaga taagtatgtc acagttaaaa
     7081 aagcacctag cggttcaggt aagtatccag ttgtacgtca atcatctgtt gctgcacttc
     7141 ctgaagttga agaattagct gaaaacccag aattagcggt taaaccgttt tatcaattgg
     7201 tttatgacat taaaacgcat cgtggttact tccgtatttc acgtgaatct attgaagata
     7261 gcaaagttaa tgtactacaa gaattgaaat tatggatggc gcgcacaatt gctgcaacgc
     7321 gtaatcaagc aattattgat gtgttgaaaa atggttctca aggagaaggt ggcaagcaat
     7381 taaaattaga aaaagttgct gcaaaaggta tcgacgggtt gaaagatgct gttaacctta
     7441 acatcaaacc aaattacgag cacaatattg ctattgtatc tcaaacaatg tttgctaagt
     7501 tagataagat gaaagacaaa aacggaaatt acttaattca accagatgtt aaagaaaaaa
     7561 cgcaacaacg tttactaggt gctaaagttg aaatcttacc agatgaaatg ttaggagaaa
     7621 aagcaaacga gacattgatt ttcggtaatc taaaagatgc aattgtattg tttgaccgtt
     7681 cacaatatca agctggctgg acagattaca tgcatttcgg tgaatgttta atggtagctg
     7741 tacgtcaaga ttgccgtatc ttagatgaaa aatctgcaat tgttattaat tatgaagata
     7801 cgaaaaatgt tggagacgtt agtttagaag cgtaagtacc tattaaaaaa tatataaaga
     7861 ggtgaaagct tatggcgatg ttcaaagtaa agaaatctta tactgactta gaaaaagggg
     7921 aatatctgga aagcggtaaa catgttgaaa tgacagtaaa gcgtgctgat tatgtcaaca
     7981 aaaagctgaa agaacacggt gtaatactag aacgtgtcaa agaagaatag gtgatttgat
     8041 gcaattgaca accactgaac taaagttact aaaaatgcat tgcaaaatag atcataactc
     8101 tgaagataaa ttactagaaa cctattatag ttgggctttt tatgaaatag tcagtgctgt
     8161 tacagatgat tatattgaat acgaagactg gtttaaaagt aaccctcttt ttactcgtgc
     8221 tgtataccct ttggctaatt attattttga aaatcgtatc gcttatcagg atagaaattt
     8281 atcacttgca cctcatatgg ttttaagtac tgttcataag ttgagagatt catttgaacg
     8341 atatttggag agtgaagaag atgaaattta attcaaacaa attaactgag cgtgtcgatt
     8401 tttgccaaga tatcagcgaa cgagtaaatg gtaatccagc aaaaccaaag tcgaaagttt
     8461 tatattcatg ttatgcatgt atacgagaag ctaaagaatc tgatacacag accaacttga
     8521 atacaggtag taaatttata aaaactatta ttatcagaga tcctagaggc gattataaac
     8581 cctcaaacaa acattacatt acgcacgaaa gccaaagata taacattaag tatgttaaat
     8641 cggattatca agataaatct tatctacgtg tgtatggtga ggtggttata tagtgggagc
     8701 taaaattgag gaaaacacca ttgaacaggg tttaaagaat gcagttttaa aaatgaatct
     8761 gaacggcaat gcgattatta aagctggggc tatgtcatta gtcccacttt taaaaagtaa
     8821 tacacctttc gctgacacca aaaaacacgc tcgcgaacat ataggtgtct ctaatgtgaa
     8881 aacagataga gactcaagcg agaaaatagt tacagtaggt tatacaaaag gtgtttctca
     8941 tcgtattcat gcaacagagt tcggaacgat gtaccaaagt ccgcaactat tcataaccaa
     9001 aactgagaaa cagggtaaag ataaagtttt aaaaacaatg attgctactg caaagaggtt
     9061 gcaaaaatga ttaacattac taatttgatt agaaatacaa ttattaaaga aaatgttaca
     9121 gaagaatcac atgtatttaa ctatacagta gatgaccatt ttcacgaaaa aaccaataag
     9181 ccaattgtgc ggatataccc actaccattt aaccctgatg aatacgctga tgatagtgaa
     9241 tttactaggg agtataatta tcaaattgat atttggtggt ctgaggatga accgaatgaa
     9301 caggctgaaa aaatagtaga gtcgctaaag aaagcgaatt ttcaaagtta ctatagagaa
     9361 ccgttatacg aaagtgacgt catgtcattc agacatatta taagagcaaa aggctcgatt
     9421 ttatcaatga aattggagga aaattaaatg attgaaaaat tgaaacaagc accaagattt
     9481 ttaaaattaa acttacaaca ctttgcggat actggcgttt caggtattgc gattggtgta
     9541 tctaattttt attatgcgcc gattttaaaa gatactgaaa aagaatggga aacaggcgct
     9601 gggacacgaa ttcgattttt aaaagagatt gaagtagata gaccacaaga tactgaagaa
     9661 gattacggag atgatatggt tgctgcaact gctgtatcta atggtaaatt gagtgttaaa
     9721 acaacatttg ttactgttcc tgctgacgat aaagcattct tgaatggtgc taaaaaaggt
     9781 acaggcggtt ataaatatgg agctaaggat attccaccag atgtagcaat tgtgttcgaa
     9841 agacgtaatc atgatgagtc ttctgaatgg gttggtctat tcaaaggtaa attcacacgt
     9901 tcaagcatca aaggtcaaac aaaacaagat aaggttgaat tccaaaatga cgatgtagaa
     9961 ggtaacttta ttgatcgttt gtttgatgaa agttcacatg tcactggtta tgatgcaaaa
    10021 ggaagcacta caggtcgtga ctatgtattc atggagacat ttggtaaaac ttatgatgaa
    10081 ttcatgtcta gtcgtggtga acaaactaca gaatctgtag aaaaagaaat gaaaaaaact
    10141 gaaaaagttg aagtaaaatc tgtaaacata agcgatgaac aagttacggt aaaagttgat
    10201 gaaacaaaac aactttcagc tacaactgaa ccatctggac agaaagtgac ttatgcagta
    10261 actgaaggac aaacgtatgc tagcgtttcg tcatcagggc ttgttaaagg tttagcggaa
    10321 ggtagcgcaa ccgttactgc gacttcaggc aagaagaccg acacagtgca agttacagta
    10381 caatctaatt tagaaatgta aacgtgaggg cttaacgccc tctttttatt ttggccaaac
    10441 taaaaaagaa agtaggaaat taataatgga acgtacatca attgaattaa ttacaggatt
    10501 tacaaaaaca ggaaagcctc aatatcaaaa atatttagca aaaccaatta ttactttgtt
    10561 tgaaacaatt caaggttcaa aattaggttt aaaacttaac aaagcgttta agggttctga
    10621 ttttaaagag ttaacagaag aagaatttaa taacttaagc gtgacagaac aagaagaata
    10681 caaaaataaa caagaagaaa tcgaagacaa catggcttta caaatggaag tgctagaaga
    10741 agttttggat ttcattgttg aagcttttga caatcaattc actagtatag aacttcaaaa
    10801 aggattgcca aatggtcaag aaggtattga aaagattgga cagttaattg gacgtatcac
    10861 aggcggggaa cctagtgata caaaaaagtt cgtgacagag aatcagaagt aagaaaagaa
    10921 gatttaacac ctgaagctgt ctacaacaat tacaggaaaa tagctaaaga tttgatagaa
    10981 aaaggcatgg atgcagaaaa agtggctaac atgccgatac acttcttttt agacattgtc
    11041 gaatcgaaga ttgaaacaaa gcgaactgcg aaaagtttta aagatatttt ttaatcagcc
    11101 tttaaaggtt gattttttat ttacatcttg gaagaaagga ggtttttaaa tgcctaatcc
    11161 tataggtaat atggtcataa aggttgattt agatggttct ggattcaata gaggtgtgac
    11221 aggtttaaat aggcaaatga aaatggtttc gcgtgagctt tcggctaatt tatcacaatt
    11281 ttctagatat gataattcat tagaaaagtc gaagataaaa gtcgaaggtt tgagtaaaaa
    11341 acaaaaagtt caagcccaga ttactaaaga gctgaaagat agttatgaca aacttagtaa
    11401 agaaactggt gaaaacagtg caaagacaca agctgcggct gctaaataca atgaagctta
    11461 cgctaaatta aaccaatatg agcgagagtt aaaccaagcc acacaagaat taaaagacat
    11521 gcaaagagag cagaaagcat taaatactgc aatgggaaaa cttggtacca actttaataa
    11581 ttttggtcct aaacttcaag aaattggtaa cagtatgaaa aatgtaggcc gtaacatgac
    11641 tatgtatgta actgcgccgg tggttgctgg gtttgctgta gcagctaaaa aaggtattga
    11701 attcgatgac agtatgagaa aagttaaagc aacttcaggt gctactgggg aagagtttga
    11761 agctttgaag aaaaaggctc gcgaaatggg tgcaacaaca aaatttagtg catcagattc
    11821 ggctgaagca ttaaattaca tggcacttgc tggttgggat tctaagcaaa tgatggaagg
    11881 tttaagcgga gttatggatt tagcggcagc atctggcgaa gaactgggag cagtaagtga
    11941 cattgttaca gatggactaa cggcatttgg tttaaaagca aaggatagtg gtcatttttc
    12001 ggacgtttta gcacaaacta gctcgaaggc aaatacggat gttagagggc tcggagaagc
    12061 ttttaaatat gtcgctcctg tagcaggtgc gttaggttac acgattgaag atacatctat
    12121 tgcgataggt ttaatgagta atgctggtat caaaggtgaa aaagcaggta cagcgttacg
    12181 aacaatgttc accaatcttt caagtccaac tagagctatg gggaatgaaa tggagcgctt
    12241 aggaatatct attacagata gtaatgggaa aatgattcct atgcgaaagc ttttagatca
    12301 actgagggaa aaatttaaac atctttcaaa agaccaacaa gctagttctg cagctacaat
    12361 atttggtaaa gaagcgatgt caggagcatt agcgattata aatgcttctg atgaagacta
    12421 tcaaaagtta accagatcta tagattcatc taccggcgca tctaaaagaa tggccgatac
    12481 aatggaatct ggtttaggtg ggaaattaag aactttaagg tcgcaattag aagaactagc
    12541 cttaacgatt tatgacagaa tagaaccagc actaaagatt atagtaagtg cttttagcaa
    12601 agtagtgaca tgggttacta aattaccaac gtcaattcaa ttagcggttg ttgggtttgg
    12661 attatttgca gcagttttag gtcctttagt ttttatgttc ggtttattta tcagcgtgat
    12721 ggggaatgca atgacagttt taggaccctt gttaataaac gttaataaag ctggtggtat
    12781 attcgcgttt ttaagaacta aaatcgcatc acttgttaaa ctatttccga ttttaggtat
    12841 gtcgatatcc agtttaacgt tacctataac attaattgta ggtgcattag ttggtattgg
    12901 catagctttc tatcaagctt ataaacgttc agaaactttt agaaatattg taaatcaggc
    12961 aatctctggt gtagcaaacg catttaaagc agctaaacta gcgttacaag gtttctttga
    13021 tttattcaaa ggtgatagta aaggcgcggt taccctagag aagatatttc cacccgaaac
    13081 tgtagcagga atacaaaatg tagttaatac gattagaaca actttcttta aagtagttga
    13141 tgaaatcgtt ggtttcgcca aagagatagg cgctcaatta gcctctttct ggaaagagaa
    13201 cggctcagaa ataacacaag ctttgcaaaa tatagctggt ttcattaaag caacctttga
    13261 atttattttt aactttatta ttaaaccaat catgtttgcg atttggcaag tgatgcaatt
    13321 tatttggccg gcggttaaag ctttgattgt cagcacttgg gaaaatatca aaggtgtaat
    13381 acaaggggct attaatatta ttttgggtat tatcaaagtg ttctctagtc ttttcacagg
    13441 aaactggcga ggcgtttggg acggcattgt aatgatactg aaaggtactg tgcagttaat
    13501 ttggaattta atacaactgt ggtttgtagg taaaattcta ggtgtagtga gatactttgg
    13561 tggattactt aaaggtttaa taactattat atgggttgct ataataggcg ttttcaagaa
    13621 atcattatcg gcaatttgga atgcaacaaa aagtattttt ggtttcttat tcaatagtgt
    13681 taaatctatt ttcactaata tgaaaaactg gttatctagt acgtggaata atatcaaaag
    13741 caataccgtc ggcaaggctc attcgttatt tacgggtgta aggtctaaat tcacaagttt
    13801 atggaatgcg acgaaagata tatttactaa attaagaaat tggatgtcaa acatctggaa
    13861 ctctattaaa gataacactg taggtatagc tggtcgctta tgggatagag tgcgtaacat
    13921 ctttggaagc atgcgtgacg gtttaaaatc tatcattggt aaaattaaag atcatatcgg
    13981 tggtatggta gacgctgtta aaagaggtct taataaatta attgaaggtt taaactgggt
    14041 cggtggtaag ttgggtatgg acaaaatacc gaagttacac actggtactg aacatacgca
    14101 tactactaca agattagtta agaacggtaa gattgcgcgg gatacgttcg ctacggttgg
    14161 ggataaagga cgtggaaatg gtccgaatgg tttcagaaat gaaatgattg aattccctaa
    14221 tggcaaacgg gtacttacgc ctaatacaga tacgacagcg tacttaccta aaggttcaat
    14281 agtatataac ggcgcacaaa cttattcaat gttaaacgga acgcttccaa gatttagcat
    14341 aggtactatg tggaaagata ttaaatccgg tgcatcatcg gcatttaact ggacaaaaga
    14401 tcaaataggt aaaggtacaa agtggcttgg cgataaagtt ggtgatgtca tggactttat
    14461 cgataatcca ggcaaacttt taaattatgt acttcaagcg tttggagttg atttcagttc
    14521 tctaactaaa ggtatgggta ttgctggcga tataacaaaa gctgcatggt ctaagattaa
    14581 gaaaagtgca atcaagtggc ttgaggatgc tttcgcagag tcgggtgatg gcggtgtatt
    14641 agatatgagt aaattacgtt acttatacgg tcacactgct gcttatacac gagaaaccgg
    14701 acgcccattc catgaaggtc tggattttga ttacatttac gaacctgttc catcaaccat
    14761 taatggtaga gcacaagtta tgccttttca taatggtggt tatggaaaat gggtgaaaat
    14821 tgtaaagggc gccttagaag ttatttatgc acatttatct aaatataaag ttaaaactgg
    14881 tcaacaagtt agggtcggac agactgttgg tatatcgggg aatacggggt ttagtacagg
    14941 acctcactta cattatgaga tgcgttggaa tggaagacat agagacccgt taccgtggtt
    15001 aagaaagaat aatgggggcg gcaaaagtac acccggtggt aatggtgcag ctaatgctag
    15061 acgagctatt aaggctgctc aaaatatttt aggaggaagg tataaggcga gttggattac
    15121 taacgagatg atgcgtgttg cgagtcgtga atccaattat acagctaatg cagtcaataa
    15181 ttgggatagc aacgcaagag ctggtatacc ttcaagaggt atgttccaaa tgatagatcc
    15241 ttcatttaga gcgtacgcaa agtcgggtta caataatcct ctcaacccaa ctcatcaagc
    15301 tatatcggct atgagatata ttgtgggtaa atgggtacca agaacaggct catggagagc
    15361 tgcgttcaaa cgcgctggtg attacgcata tgctactggt ggcaaagtct ataacggatt
    15421 gtatcactta ggggaagaag gatatccaga gtggataata cctactgatc caagtagagc
    15481 gaacgaagca cacaaattat tagctttagc tgctaacgat attgataacc gctctaaaaa
    15541 taagcgacca aacaacttac caaatccaag tataagtaat agtgatacaa actatattca
    15601 tacattggag aataaactag atgcggttat taattgtttg gttagtttgg ttgagtctaa
    15661 tcaagttatt gcagataagg attacgaacc agttattaat aagtatgtgt ttgaagatga
    15721 ggtaaataat tctatcgata aacgagagcg tcacgaatct acaagagtta gatttagaag
    15781 aggaggcacg ataatctaat gcaagataca attcaaatag acaataaaac aattggatgg
    15841 ctggttgtgc aaagagggtt cgagataccc tcttttaatt ttgttactga aaaagaaaac
    15901 gtaaaaggta gagcgggatc tattgttaag aatcgttatt taaatgatat cgaatttgat
    15961 ttaccattaa ttattcgaaa cgaaaaattg tcaccaggtg gagaaaaaac acacgatgat
    16021 atattagaag cattggtcaa gttcttcaat attaaagatt taacacctaa aaaacttaaa
    16081 ttcaaatctc aaaactggta ttggtttgca tattttgatg gtccattaaa attaccgaaa
    16141 aacccaagag gttcagtgaa gttcactata aaagtagtgt taacagatcc ttataaatac
    16201 tcggtaactg gaaacaaaaa caccgcgatt tcagaccaag tttcagttgt aaatagtggg
    16261 actgctgaca ctcctttaat tgttgaagcc cgagcaatta aaccatctag ttactttatg
    16321 atcactaaaa atgatgaaga ttattttatg gttggtgatg atgaggtaac caaagaagtt
    16381 aaggattaca tgcctcctgt ttatcatagt gagtttcgtg atttcaaagg ttggactaag
    16441 atgattactg aagatattcc aagtaatgat ttaggtggta aggtcggcgg tga
//
LOCUS       DAKSQE010000057        16485 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.057, whole genome shotgun sequence.
ACCESSION   DAKSQE010000057 DAKSQE010000000
VERSION     DAKSQE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16485
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.057"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..417
                     /locus_tag="O0V33_001574"
     CDS             <1..417
                     /locus_tag="O0V33_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HCX8976204.1"
                     /translation="LLLPNTFYMVTDLIHLNQFQFNFYAGLNLGEWKYFTYLLLGVFL
                     AVYVMILIYFEILTLTSHRWLNRLIVVVLMFLNGFGIYIGRFLRLHSVYFFNEPLHVL
                     NHILESLTLKTAMFVCFMVVMQAAILLFGKGVRLNK"
     gene            414..698
                     /locus_tag="O0V33_001575"
     CDS             414..698
                     /locus_tag="O0V33_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976205.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(853..2226)
                     /locus_tag="O0V33_001576"
     CDS             complement(853..2226)
                     /locus_tag="O0V33_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCX8976206.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVIAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYKVFTSFSKKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQIIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPET
                     SYQAFKSKYRQIKWSQNEADFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DPKALYIKTYLPIFNQIDAKYLHDTQRNESNLFELGIELGRHYPRQMVDHQEKRTQVL
                     AAFKALD"
     gene            complement(2312..3865)
                     /locus_tag="O0V33_001577"
     CDS             complement(2312..3865)
                     /locus_tag="O0V33_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HCX8976207.1"
                     /translation="MSEVKKVVKHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAVLAGSDLLGTNHALSLAFSGFATSAVALVAAALFLAAAMQETNLHKRLALLVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSIALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSAREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKTGIPLTIVGYILVIVFSM
                     TYWKWLGLL"
     gene            complement(4148..4435)
                     /locus_tag="O0V33_001578"
     CDS             complement(4148..4435)
                     /locus_tag="O0V33_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976208.1"
                     /translation="MTNEDKRFEQLRFERKFIVIPYVIYAVIVLLLNIFYSDLKITMT
                     LFGLFFAYNVVILFIAFIKHYKRTLLLSLILTVLSGAAFFGIIYVYGINHF"
     gene            complement(4470..5378)
                     /locus_tag="O0V33_001579"
     CDS             complement(4470..5378)
                     /locus_tag="O0V33_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCX8976209.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRPSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVIQSIADKYGVSDTAVMIAWLVKIPHRIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIGQDIP"
     gene            complement(5481..6407)
                     /locus_tag="O0V33_001580"
     CDS             complement(5481..6407)
                     /locus_tag="O0V33_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCX8976210.1"
                     /translation="MKNNKDEKIRISIINGFLGSGKTTLLTHYISELLKNDEKIKIIM
                     NEFGTVDIDSNSISNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIEATGI
                     AHPLELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEDQLKLSDMII
                     INKIDLVTDDSIEKIDKQLSLICASIPTYKTTYGQVSLEELDLPGKYREISSHHHHHH
                     GIKSMTYTFTGPIDRQLFYQFIMKLSESVLRLKGYVSFRDQPNAIYEFQYAYGLPDYG
                     IIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT"
     gene            6634..7077
                     /gene="mgrA"
                     /locus_tag="O0V33_001581"
     CDS             6634..7077
                     /gene="mgrA"
                     /locus_tag="O0V33_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HCX8976211.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
     gene            complement(7204..8877)
                     /locus_tag="O0V33_001582"
     CDS             complement(7204..8877)
                     /locus_tag="O0V33_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HCX8976212.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLIVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRNNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MVLTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGNKRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSIRFEN
                     MDMFEIDDKDKFEALNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAIARMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(8874..10505)
                     /locus_tag="O0V33_001583"
     CDS             complement(8874..10505)
                     /locus_tag="O0V33_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCX8976213.1"
                     /translation="MKKLTTILFQYKIFPVLMFLVSTGLGILVITQNILIADFLAKII
                     RHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGE
                     QMTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHINTALIMLITAPFIPLF
                     YIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRT
                     LTMRILRSAFLSGLMLEFISMLGIGLVALEATLNLVVFHNIDFKTAAIAIILAPEFYN
                     AIKDLGQAFHTGKQSEGASDVVFEFLEQPNNNNEFLLKYEENQKPFIQLTDISFRYDD
                     SDRLVLNDLNLEIFKGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISINQRDLNIGI
                     LSQQPYIFSASIKENITMFKDIENNTVEEVLDEVGLLDKVQSFTQGINTIIGEGGEML
                     SGGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKGTTMIVIAHRD
                     NTIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
     gene            10724..11599
                     /locus_tag="O0V33_001584"
     CDS             10724..11599
                     /locus_tag="O0V33_001584"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCX8976214.1"
                     /translation="MFIIELIKGIILGIVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGDNNQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSAKVKNPQTVD
                     QISYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQIADIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
     gene            11771..12454
                     /locus_tag="O0V33_001585"
     CDS             11771..12454
                     /locus_tag="O0V33_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976215.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKKSQKYEVKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            12457..12915
                     /locus_tag="O0V33_001586"
     CDS             12457..12915
                     /locus_tag="O0V33_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCX8976216.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVITQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEKSLLRVIHRIKELD"
     gene            12917..13483
                     /locus_tag="O0V33_001587"
     CDS             12917..13483
                     /locus_tag="O0V33_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCX8976217.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(13578..14120)
                     /locus_tag="O0V33_001588"
     CDS             complement(13578..14120)
                     /locus_tag="O0V33_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCX8976218.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEQHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(14200..14499)
                     /locus_tag="O0V33_001589"
     CDS             complement(14200..14499)
                     /locus_tag="O0V33_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976219.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGRTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            complement(14637..15026)
                     /locus_tag="O0V33_001590"
     CDS             complement(14637..15026)
                     /locus_tag="O0V33_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976220.1"
                     /translation="MKKLIISIMAIMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKEASQDKQSKDQ
                     TKSSDKDNHKKPTSTDKDQKANDKHQS"
     gene            complement(15093..15536)
                     /locus_tag="O0V33_001591"
     CDS             complement(15093..15536)
                     /locus_tag="O0V33_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCX8976221.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQAESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LEKWAKTMNAKRISSTIHKSNLPMISLNKDLGYQVSHVKMYKDID"
     gene            15663..16352
                     /locus_tag="O0V33_001592"
     CDS             15663..16352
                     /locus_tag="O0V33_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HCX8976222.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVL
                     LIGIVSFLKGFLGFFMEKSGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTIQLQCFNNS
                     NWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIVMPIGLRIER
                     NISKKHSHTFL"
BASE COUNT         5598 a         2830 c         2377 g         5680 t
ORIGIN      
        1 ttacttttac caaatacatt ttatatggta actgatttaa tccatttgaa ccaatttcaa
       61 ttcaactttt acgcaggact aaatttaggt gagtggaaat actttaccta tttactgctt
      121 ggtgtcttct tagcggttta tgtgatgatt ttaatctatt ttgaaatact aacattgact
      181 tctcaccgtt ggcttaatag attaattgtt gtcgtactaa tgtttttaaa tggattcgga
      241 atttatattg gtcgtttctt acgcttacat tccgtttatt tctttaatga acccttacat
      301 gttctaaatc acattctaga aagtctaacg cttaaaactg ctatgtttgt atgttttatg
      361 gttgtgatgc aagcagcaat tctattattt ggaaaaggag tgcgtttgaa caagtgacaa
      421 taaatataat cgttttaata gtatctatca ttgtctttca attgattatt ggacacatat
      481 ggcatgatat tggtttaagt tatttaagaa gtatcttact catgatgcta ccatttggac
      541 taggtgtatt tatacaacaa gtgtcgtatt acgaacgaca atatcctaaa tggcaagtgc
      601 ctcaaaacat aaaagttaga ttgaaatata tctatctcgc tacattttta gagtatgtcg
      661 tactatacct cactttattt acggacatac taagataaca ccaaattaat aagaaaacta
      721 aatacaactt atatatttga gcaaagccaa tttctatcac atcaataata gaaattggct
      781 tatttatatt gcttaagaac tttaaattct gctccagatt ttactcatgc ctgatctatc
      841 aattcagccg aattagtcta gcgctttaaa tgcagctaaa acttgtgtac gtttttcttg
      901 atgatctacc atttgtcttg gataatgacg acctaattca atccccagtt caaaaaggtt
      961 ggactcattg cgttgtgtat catgcaaata tttagcatca atttgattaa aaatcggaag
     1021 atatgttttg atatacaaag ctttaggatc aaagcgttca ctctgtctta ttggattaaa
     1081 cattctaaaa tacggcactg catccgtacc tgtagaagca gaccattgcc atccatgaat
     1141 atttgatgct gcatcatagt caataaggtg ctttctaaag aatttttctc cccatgtcca
     1201 atctataaat aaatctttgg ttaaaaattg cgacacaacc attctcattc gattgtgcat
     1261 aaaaccagtt tgtgtcaatt ccattattgc tgcatcaatg attggaaatc ctgtttgccc
     1321 ttcgcaccat gcattaaaat ccgcttcatt ttgcgaccat tttatctgtc gatatttaga
     1381 tttgaaagct tggtatgagg tttctggata ctgtgtcatt aacacataat aaaattctct
     1441 aaaaatgagt tcacgtataa atgcttcata gtttgcctca tcctcatcat aacgcgccaa
     1501 taaatcatta aaaatttcaa taatatctaa taatccatat gctaaagcaa cacttagctg
     1561 acttgttaac acttcaggta aatagtctct tccgctttcg taattctgta tatcttgatc
     1621 taaaaaatgt tgccatttat tctgttcaat tatttgtgag gatccaaatg ctatgtcatc
     1681 aaacactaaa tcatcaggtg atgcaatgac gaagtcttcg aatgatttta aatcatagtg
     1741 atatacgtct ctatgcctca agtaaggtcg ccatttttta gagaaactag taaaaacttt
     1801 gtatggctcc ccttgttgat tcattgtttt actcgcttta aagtaatgat tcccacgtat
     1861 caaagtaacg gcaatgcgtg cttcattgaa taaagagcgt tgatgtaaaa tatcatattc
     1921 ttcaaggtga taactcataa tatcacctgc aatcactact tcagagatat tgttttcttt
     1981 acaaaatgaa atgatatcgt cataagacat gacataaggt gtgagctttt ctgcgtgcaa
     2041 cgattggtaa aagcgctgca aagtaccgcg ataaaactgt gctttcaaat cagaagctga
     2101 agacatgtcc tcttccggaa tgataaaata acatgcatca atgtcttctt tttgttgata
     2161 aatatattca aataatggat tgtgttccat tcgaaacatt cgatttaata acacagctat
     2221 tgccataaac aaaacaccct tcaattatct ttttatttta ttaaactttt tgtcacttta
     2281 aaataaccta gattcttatt tatattttaa attaaagcaa acctaaccat ttccaataag
     2341 tcatactaaa aactatcact agaatatacc ctacaattgt caatggtata cctgtcttca
     2401 agaaatcttt cacagtaaaa gtaccagtgc cataagccaa catattttga ggtgcactaa
     2461 caggtaataa gaaaccaaaa ctaataacaa attgttgaat taaaacaaat cctatagact
     2521 ggtctcctaa gtgtaacgta gaggttagcg aaataaaaac aggtattaac gctgatgata
     2581 aacttgttgc actcgcaaag cccaaatgaa tcaatatatt aaaaagcgtg ataagtgcaa
     2641 ttgtcgcgat aataggtaaa tgttttaaac ctaaaacacc aaaagtttga tcacttaacc
     2701 attgagctgc tcctgttttc aaaagaacgt tacctagtga aatacctaca ccaaacacga
     2761 taattgttcc ccatggtatt ttattttcaa catgtttcca tgtcatgaca ccaattttcg
     2821 gcattaacat aacacctaaa gcaataatag taatggatgc agagtcaatc ggatgtaata
     2881 ctttttcagt tgaccaaaac agtaataata acatcgatat aacaattaaa cgccattcac
     2941 gtgcgctaac ggggccaagt ttatgcaatt cttcttttat taaatctttg ccaccttcta
     3001 ttgtattaat ttctggaggc atcactttaa tcatgatgaa atataaagct atagacataa
     3061 ctatggacca aggcgctgca tataagaacc actcgcccca tgaaacatca aatcctaatt
     3121 gatggtttat aaaattaatc gctacgatat tttgtgctgc cgccgttttg ataccaatat
     3181 tccaaattga cacagcttgt actgaagtaa ttattaataa agacgctaac ttgctatctt
     3241 tagaaacttt aaatgctgca atcataccca gcaagattgg tacaactgcc cctgctctag
     3301 ctgttgcaga aggaacgaaa aatgcaagta caattgaaac aataattgct ccaataacta
     3361 tatttctagt tttattacca acaattgata gcactaaaag agctagtctt ttatgcaaat
     3421 tcgtttcttg catagcagca gccaaaaata atgcggctgc aacgagagct acagctgatg
     3481 ttgcaaatcc actaaatgct aatgataatg catgatttgt tcctagaaga tcacttccag
     3541 ctaaaacagc actgccactt ttcggattac ctagcttctc ccctaaattt tgaactggac
     3601 taaatcctaa aagtaatatc attaaaccaa taattaaagt tgctgacacc ggatatgata
     3661 cagcttccgt tacccacata ataactgcaa aagctaaaat agctagtact gccttagcca
     3721 ttattggcag acttgaaggt gccgggagta atagtacaac tataagtatt ataaaactca
     3781 taacaatcca aaatggcttg tattgctttt ttgtcttttc tttcttttgt tgttcatgtt
     3841 taactacctt ttttacttct gacataacag tccccccttt gtttaatctt aatttaatta
     3901 taacaatttt tataaacatc cttttttgat ttatgcaaaa tacacaaatt tatcgaaata
     3961 ttttgtaaat aatattgcta ggtgatgagt tatctcggtt aattacaaaa cgatatctag
     4021 gtttaaatat tagatcataa aaaaactatc tactgaatat cctcatagca tcatttttct
     4081 aagcaaaagt taaacttagt tagataaaat gcaaatgaga ttattgtaga tagtctcttt
     4141 ttaatattta aaaatgatta atgccataaa cataaataat tccaaagaat gccgcgccac
     4201 taagcactgt taatataaga cttagtaaca atgtgcgttt ataatgttta ataaatgcta
     4261 tgaacaaaat gactacatta tacgcaaaga aaagtccgaa taatgtcatt gttattttca
     4321 aatcagaata gaaaatattt aatagtaata caatgactgc ataaattaca tacggaataa
     4381 ctataaattt gcgttcaaat cttaattgtt cgaaacgttt atcttcgttt gtcatgatat
     4441 gctcctttat gatttaaaag taaataagtt tacggaatat cttgtccgat aatagcggtg
     4501 taaatgtcaa accacgactg atcatctaaa ttaagttgca gcccttcgat tgcttgatca
     4561 atacgcttta actgacttgt tccaagtatc ggcatgatac gatgcggtat ttttactaac
     4621 catgctatca tcacagctgt gtcactcaca ccatatttgt cagctattga ttgaataact
     4681 ttcataatac gttgcgcttt aatatcttcc ttgtcgaaaa ttttaccgcc tgcaaaagga
     4741 ctccaagcca tgatttgaac atggttttga tacattgaat ccattgttcc atcttgtaaa
     4801 ctatcaacgt gatatggcga taattctaat tgattgatgc taatatgtag tctttctttc
     4861 ataatatatt gatttaacaa ttggtattgt gaatgattaa aattcgacac cccgaatgac
     4921 ttcaacttac cttgtttaac aagtttagtt aatgcatcag caacttgttc tggatccatc
     4981 aatggtgaag gacgatgaat gagtagacta tctaaataat ctacattcaa attgattaat
     5041 gactgttcaa cagatttcac gatgtgctta ctactcaaat cataacgatg tccatttgta
     5101 aaatcaaatt gcttagaagg caaaatgata ccacatttcg taacaatttg aattttattt
     5161 cttaattcgg gtgataaatc caaagcatta ccaaacagtg attcacattg ataatcccca
     5221 taaatatcag catgatccat cgttgtaatc ccacgttcaa ctaattcatt tataaaataa
     5281 tttaactctt tcgcagtcat cttccattca tttgcacgcc aaaaaccttg tacaagccta
     5341 gaaaaatgaa catagtgatt aatcattatt tgttccatat ctcatccact ccatccctca
     5401 atgtcacaaa atattcacaa taaaagtatt aagaaattaa tgtgctattt ccgttaaatt
     5461 gaaacaacac attttctgat ttacgtaaat tgtagcatat ccaattgatt acgtatgtga
     5521 tttgtatcta aagtttcacc aataataaca atcgttaatg gtaattgcat gccaattatt
     5581 ccataatctg gtaaaccata agcatattga aattcataaa ttgcatttgg ttgatctcta
     5641 aatgatacat aacctttcaa acgtagaaca gattccgata atttcattat aaattgataa
     5701 aacaattgac gatcaatcgg acctgtaaat gtgtaagtca tacttttaat cccatgatga
     5761 tggtgatgat gcgacgatat ctctctgtat ttaccaggta agtccaattc ttccaacgaa
     5821 acttgtccat aggttgtttt ataagttgga atacttgcac aaatcaaact taattgctta
     5881 tcaattttct caatactgtc atcagttaca agatcaattt tattaataat aatcatgtca
     5941 cttagtttca actgatcttc catcagcgaa actgtatttt cggtatattg atgacgttcc
     6001 aaaaatcgag tcgcatctaa tacaccataa ataattggct tttcaaagaa attaacgatt
     6061 tgcggatctt gacatgcaac tagtaattct aaaggatgcg caatgcctgt cgcttctatg
     6121 atgacatgat taacgtcccc ttttaaagca atggctttta gttcatagac aagttcttgt
     6181 ttaagatcgc aacaaacaca accattaatc aatgaatgga cttcaatttc atttgaaata
     6241 ctattgctat cgatatcaac agtaccgaat tcattcatga tgattttaat tttctcatca
     6301 ttttttaata attcactaat ataatgtgtc agtaacgtgg ttttaccact acccaaaaat
     6361 ccgttaatta tggatattct tattttttca tctttattat ttttcatttt atattctctt
     6421 ttctattaca gttattttta aaaatgataa catatatatt aaattatctt tttgcttgat
     6481 ttaatcaaaa atatcttcta aactttaaac atactttaat tttagcatgg cattgaatca
     6541 aagaaagttg tgaattaata aacaatcaac tttttaatga ttaccaatcc gaaaagagaa
     6601 aaaaacggat agtatgtctg gaggagaact ttaatgtctg atcaacataa tttaaaagaa
     6661 cagctatgct ttagtttgta caatgctcaa agacaagtta atcgctacta ctctaacaaa
     6721 gtttttaaga agtacaatct aacataccca caatttcttg tcttaactat tttatgggat
     6781 gaatctcctg taaacgtcaa gaaagtcgta actgaattag cactcgatac tggtacagta
     6841 tcaccattat taaaacgaat ggaacaagta gacttaatta agcgtgaacg ttccgaagtc
     6901 gatcaacgtg aagtatttat tcacttgact gacaaaagtg aaactattag accagaatta
     6961 agtaatgcat ctgacaaagt cgcttcagct tcttctttat ctcaagatga agttaaagaa
     7021 cttaatcgct tattaggtaa agtcattcat gcatttgatg aaacaaagga aaaataatta
     7081 acttttgtca tgacaattaa agtaatgttt agaatttatt aagaatagaa aaacaattag
     7141 cacgcgtaag cttgttagtt aaaaaactgc ttgaaaggtt tcttagccta tcaagcagtt
     7201 tttttatgca ttatattgaa tcatattcca taaagcacct tgattagcga gtaattgttg
     7261 atagtttccc ttttcaacta ttttaccatt tatcatcaca atgatggtct caaaacgtga
     7321 caataaagtt aaatcgtgtg tagcaacaat taatgtttct gcatgtgctt caattaaatc
     7381 cataactttc aaactatttt gtttatctaa tgccgttgtt ggttcatcta aaatccacgt
     7441 tgatgcagta tcttttaata acatcctcgc aatcgctaaa cgctgaattt ctccgccaga
     7501 taatgtatga ccatctaagt caatttgacg ttctagtgcc aaatgttcta aatctaattg
     7561 cttaaatatt gcttgcaccg cttcatcttt ttcatcggta aataaatttt gacgtattgt
     7621 accatcaaat aattgttgag attgtagcaa gacatttaac gcttcaaact tatctttgtc
     7681 atctatttca aacatatcca tattttcgaa acgaatagag ccactatcta attgatataa
     7741 ccctgccata atttgtagta atgtactttt tcctgaacca gaaggaccca caatcgcaat
     7801 cttttcgcct ctattaacat taaaattaat atccgacaac acatacgttt gctggttcca
     7861 atacttaaaa ctagcgtcct taacttcata aatgttcgtt gcatcatact tatcattaag
     7921 gcgtttattt ccattggtag aaggtacaga tatcacttca ttaatatcgt gcaatgcttg
     7981 gtcagtatcg gctttataat acgcgacatt tgtcattggt acagcttgtt caaataaagt
     8041 taagaccatt aaaactatac tcgtcatata aataatattt agttggcctg cattaatttg
     8101 aattaatcct agaactaaac taccaaaaat cgaaagcata gcaataatat ttaatataaa
     8161 atcataaatc gttaaaaagc gttgctcttt aagttgtaat ttatcaaaat gatttaattt
     8221 agccatcaaa ttatttcgat aatgattaat ttgattaaat cgacgtagtt catccatacc
     8281 agctttataa tcataaaaat gatttaaaaa tcgagcctgt tcattagctg catgtttctt
     8341 taaagtacgt gctttttttg agcttaacca aggaacaatg agtaaagtaa acaacatgct
     8401 aaccataatc aatagcgcat ggccgattga aatgaacgcc aatactatta ctgtaactag
     8461 cgctgtcaaa ccgatgacga ctggtggata ataaacacgt aaataaatat tttgtaacgc
     8521 ctcaacacga ctaatcatac gtgaaattaa atcactagaa ctcagtttac ggtaaacatt
     8581 aggaatgaca tttactaatt taccgaaaaa ctgtacccga atatcacgta gcattgtaaa
     8641 tgtagcttta tgagaaataa ggcgctctac gtatcgagta atagctctta aaaacccaaa
     8701 caattttact gtaacgacta aaatcatcag agcgtatagt ggcgcaccaa gtgcactttg
     8761 tgtcaccata taaccactta agaaaaacat ggcgagcgca actaaacttc cacaaacacc
     8821 aacaactata gctaacaata aatccttatc tacttgaaat tttagtcgtg ttttcataag
     8881 tcatcaccat tttctgttat atttactgaa atattgcgat catcagcaat cagtcttcca
     8941 ttttctatat acaaacgtcg ttgtaaatgg cgaattgtat tatctctatg tgcaatgaca
     9001 atcatcgttg tacctttaaa atgttgaaac agaacgttct gaatcatgtg ttctgtttga
     9061 atatctaaac cagttgcagg ctcatcaaat ataacgagat ctggcttcat aactaaaaga
     9121 cggcaaagtt ctatgcgtct catctgtcca ccagataaca tttcgcctcc ttcacctatt
     9181 attgtattaa tgccttgtgt gaaagattga actttgtcta ataaacctac ttcgtctagc
     9241 acttcttcaa cagtattatt ttctatatct ttaaacatcg taatattctc ttttatagaa
     9301 gcactgaaaa tatatggctg ttgactaagt attcctatat ttaaatcacg ctggtttata
     9361 cttatagtac ctattgttgg ctgataaaca cctgcaataa gatgtgtcaa agttgattta
     9421 cctgccccgc ttggacctac aagtgcaatt tgatcacctt taaatatttc caaatttaaa
     9481 tcatttaata ccaatctatc agaatcatca tatcgaaatg atatgtctgt taactgaata
     9541 aatggttttt gattttcctc atactttaat agaaattcat tattattgtt cggttgttct
     9601 aaaaactcaa acacaacgtc actggcacct tcactttgtt ttccagtatg gaatgcttgt
     9661 cctaagtcct taattgcatt ataaaattca ggcgctaaaa taatcgcaat tgccgcagtt
     9721 ttaaaatcaa tattatgaaa tactactaag tttagcgttg cttccaatgc aaccaatcca
     9781 atacctaaca tacttataaa ttcgagcatt aatcccgata aaaaagcact gcgtaaaatg
     9841 cgcattgtta aagttctaaa ctgagtacta tcgtcgtaaa tatgcttctc tgtttgctct
     9901 gtacgattaa atagctttaa cgtcactaaa cctttagcaa tatttaaaaa ccgttgacta
     9961 aattgattca aataagtcat ttgatctttt gactcatctc gcgttttcaa accgaaaata
    10021 atataaaaca aaggaataaa tggtgcagtt attaacataa ttaatgcggt attgatatgg
    10081 atgaaaaaca ttgcaatgat tatgatgagc ggaaccatca ttgatttgaa cacttgaggc
    10141 aaataactct tataaaaagg tgctagacca tcaatgtttt ctgtgagtat agtcatttgt
    10201 tcaccgattg gatgaccatt atttttataa ataacccgct gtctaagcat atgcttaact
    10261 ttaaatgcta atgtatcacc taaccattga tttagaaatt gcacagttgc tcttaaaagt
    10321 aaaacaccta ataaaataaa taatacaatc cataaacctt gaaattgatg tcttataatt
    10381 ttagctaaaa aatctgctat taaaatattt tgcgttataa cgagtatgcc gagaccagta
    10441 ctgaccaaga acatgagtac cggaaaaatt ttatattgaa acagtattgt tgttaatttt
    10501 ttcacaatta tatcacctaa acctattata aaaggttttc cttaaaatca aattaaatag
    10561 ctcaataaaa tattgtagtt ttacatgtaa aagaaaatga atatatacat ctttagtcta
    10621 gtattataac tataactagc atgtctttaa atgaatatgc tatcatgata tggtaatttt
    10681 gatatttaaa taaattaaat tgagtaatga aagtgggtac attatgttta tcattgaatt
    10741 aataaaaggt attatcttag ggatcgtcga aggattaacg gaatttgccc ctgtttcctc
    10801 tactggacat atgattctag ttgatgatat gtggttaaaa tcatctgaat ttttaggttc
    10861 tcaatcagca tttacattta aaatcgtcat ccaattagga tccgtctttg cagcagcatg
    10921 ggtgttccgc gaacgcttct tagagatttt acatattggt aaacacaaac atgttgaagg
    10981 agataacaat caacaaagac gttcaaagcc aagacgttta aatttattac atgtattagt
    11041 gggtatggtg ccagcaggta ttttaggctt actatttgat gatttcatcg aagaacattt
    11101 atttagtgtg ccaactgtta tgattggctt attcgtaggt gctatctata tgattattgc
    11161 tgataaatac tcagctaaag ttaaaaatcc acaaacagta gatcaaatca gttatttcca
    11221 agcatttgtc atcggtattt ctcaagctgt agctatgtgg cctggtttca gtcgttcagg
    11281 ttcaacgatt tcaactgggg tattaatgaa attaaatcac aaagctgcat cagactttac
    11341 atttattatg gctgttccaa ttatgttagc ggctagtggt ttatctttat taaaacatta
    11401 tcaagatatt cagattgctg atataccttt ttatatttta ggctttttag ctgcatttac
    11461 tgttggacta attgcaatca aaacattctt gcatcttatt aacaaaatca aattaattcc
    11521 atttgccatt tacagaattg tattagtaat ctttattgct atactttact tcggatttgg
    11581 cattggtaaa ggtatttaat ttccttttaa aatatagact gtcaattaag gaaccacttg
    11641 attggcagtt tttattgtct tctttgaaaa aacatctgaa atacttataa gtgattcact
    11701 actctcatcg tttatattga tgtatcatag ggtatgtaaa attaataaag taattatgga
    11761 ggcaagatct atggataaga aaaaagtcat caaatttatg attaatgtat taccaattgt
    11821 attggtaccg ttaattgttg aacgtaaacg tatcaaacaa catccggacg tacaaaaagt
    11881 tacagatgct acaagtaaag ttgcttcaaa aacatctgca gcaatcagta acacagcgag
    11941 tgatgttaaa gaatatgtcg gcgataaaaa acaagatttt gaaaataagc gtgaacttaa
    12001 aaagtttgct agagaacatg atcctgccta tattgagaaa aaaggcgaaa aattagctaa
    12061 acaaaatcgt aaagacgctg ataaaatgaa taaaatactt caaaaaaata tcgaaaagcg
    12121 tcataaagaa gagcaaaaag cccgcgaaaa gaatgaaata caacgtatta aagatatgaa
    12181 aaagtcacaa aaatacgaag taaaagcagg cttaacacct aataaattag atgagaaaac
    12241 tgagaaaaaa ggcgataaac tagctgaaaa aaatcgcaaa gaaatcgcta aaatgaataa
    12301 aaagttacaa aaaaatattg aaaaacgaca caaagaagaa caaaaacgcc aacaagaagc
    12361 tgataaagca cgcatcaagt catttaaaaa atataaagat tatgttgcca aaagcgcctc
    12421 tcaacaaaat aaagaaaaca atacagaggc ataattgtga cacatattat tattgatgga
    12481 gatgcttgtc ctgttgttga ttctattata gatttaacaa ctgagacagg catttttgtg
    12541 acaattattc ggagcttcag ccatttttcg aaccaattat atcccccaca tgtatcaaca
    12601 ttatatgttg atgatggacc agatgcagtt gattacaaaa ttgttcaatt atcaacgaaa
    12661 gatgatatcg taatcactca agattatgga ctcgcaagtt tattagtcga caaagtctta
    12721 attgtcatgc atcataatgg gaagatatac aattccaaaa atattcaaca actattagat
    12781 aaacgatata tgaatgcaca aattagaaaa caaggtggtc gccacaaagg tcccccacct
    12841 tttacaaagc aagatcaaaa agtgtttgaa aaatcactat taagagtcat tcatcgaatt
    12901 aaggagctgg attaatatga agcgaatcgc agtttattgt ggtgcaagca aaggtcatga
    12961 cccttcatat gtacaaaaag catacgattt aggtaaatat tttgctgaac aaggttatga
    13021 attagtattt ggtgcagggt caattggtat tatgggtgcc attcaagatg gtgtattaga
    13081 tcatggtggc aaagctattg gcgtcatgcc taaaatgtta gatgaacatg aaatcacaag
    13141 ccaacgttta actgaattaa ttttagttga ttctatgcat gaacgtaaaa ataaaatggc
    13201 agaacttgca gatgcatttg tcatggctcc tggtggcgca ggttcactag aagaattttt
    13261 cgaaatgtat agttgggctc aaataggtat ccatgaaaag cctattgcca tttataatat
    13321 taatggcttt ttcaatccac tacaaacaat gattgaccat atgattgagg aaggttttat
    13381 tgatcctaag tatcgtgcac ttgcaccgtt atgcgacact aaggaatcat taatagaatc
    13441 aatattaaat ttcaaacctc taggtactcg ttcatacgat taatcattct aaggttaaaa
    13501 aataaacaaa cacccctaat attgttatca tcagctaaac aatattaggg gtgtttgtta
    13561 cgcttatgta taaattttca ctttcgaaga ttaatgtaat agcgcacttg cctttttaat
    13621 aaatgatact tacttagatt gggtaattcg aaatgatcat acactgtcat gccaattttt
    13681 tccattacac gttgtgaagc tttatttgct tctgctgtaa aactatagac atcgtatatt
    13741 tgatgttgtt ctgctaactt caacgttgcc tttgcgcctt cagttgctaa tccttttccc
    13801 caaaattctg gcaacaagcg ccaacctatt tcataaagcg gtaattcttt aaatggataa
    13861 tcgcttgttt ctggaatata attcaaacct ataaagccta tccattgatg ggactcttta
    13921 tcttctacag caaataatcc aatgccataa tctttaataa ccgcatccat agttctcata
    13981 tctaattctg aacgacgata actcaataaa cttggaaaat atttacgtac gtcataattc
    14041 gcattcattt tttgaaaagg taacagatca tcttcatgcc agtctcttaa tattaaacgc
    14101 tctgtttcac aataaatcat cgattgacac tcctatataa tttctcaata aaaaacacaa
    14161 aatcgatatt ttgatcaact ttgcgtttta gatagcagtt tattttgatt tagaaatata
    14221 gcttataagt tcaacatgaa ctggcacttc ttttaaacgt gctctatctg atttcagcgc
    14281 agtaaaagtt gcatctataa aatcttgttg atggtttaaa cgatatgtag caacaaaagt
    14341 atctgtatcg gactcaaagt ttggtaaatg tgcaattaca cgcttaattt cgccagtaga
    14401 atcagcaatc gtgcgtcctg taatattttc taattctcta cctaattctg gcaaagtaat
    14461 atcttgaccc tctaaaattc taaaatcttg ttcatgcatg ccgtttctct cctttattaa
    14521 ttacgttgtc tatttttacg ttctgtttca atcatgacca cattttaatt aatacatacc
    14581 cttaaaataa ggtttaaaac gctgtacctt tcatctataa caagtaagca attcaattat
    14641 gattggtgtt tgtcattagc tttttgatct ttatctgttg atgtaggctt tttgtgatta
    14701 tctttatccg atgacttagt ttgatctttt gattgcttat cttgtgatgc ctctttatct
    14761 ttgtctttat ttttgtcttc gtctttttta tcgtccttgt tatcttcttt tgaaggtttt
    14821 aaatctttca cttctttttc aaggtctttt tgcttatctg ctaatttttc tttttcttgt
    14881 tgaagctttt cttttttatc ttttaattct ttattttctt tttgtaaatt atcgatatcc
    14941 ttttccagag tggctttctc ttggctttta ccacaacctg ttaaaaatag catgatcgcc
    15001 ataatgctga tgattaattt cttcatataa aactccttaa cgtcattaaa tcttttttaa
    15061 ttttaacatg aattagcaac ataatcctaa ttctaatcaa tatctttata catttttaca
    15121 tgactcactt gataacctaa atctttattc aaagatatca ttggtaaatt acttttatga
    15181 attgtactgc ttattcgctt tgcattcata gtttttgccc atttttcaag cgcaatcttc
    15241 agttgcgtag ctattcccag tttgcgaaat tgtggttcaa catatagcag ttcaatgtta
    15301 accatacttt tttcattact aaaatgtccc caaataaacg ctattaattg accttcattt
    15361 tcatatatat aaattttatc atttgtatgt tctaatcttg aacatatcat ctcgtaccta
    15421 agagcaatac tcagtttagt acatttatag ttggattctg cttgcgacaa taatgtctca
    15481 tgtatattag ctatttgctt gatataatta tgttcatctt tattaagtgt cctcattttc
    15541 ttctccgata ttaatcgtta tatttatgcg tttaatatga tatgattgac cttattaata
    15601 ctataaaatc atttccattt tagtttggaa ttaaacattt ttaaaggagc gctttagtaa
    15661 caatgaaaag tactgcgcaa ttaacaaaag aaaataatgt taaatcatta agattaagca
    15721 atactgatag agaaatcttc gagaattata tgacttatat gcgctctgac tttcgcgtca
    15781 atcctcatga tacagagtta attattaacc gtatacttaa acaattatta agcgcagaac
    15841 aacatggttt attagcctta gattttttca atcacgaccc aaaagcacat gcaataaaag
    15901 aactaaaagc gatgcctaat gaaacattta aaaatatttt taaatatata tatcagcaca
    15961 tcgttctatt aattggtatt gttagttttc tcaaaggatt tttaggattt ttcatggaaa
    16021 aaagtggaag taatttatat tttgtttcat tcccgttttc agtagtcgtt ggattcttta
    16081 ttgtcttttt atttatatgg tttagtttta aaacaattca actgcagtgt tttaacaatt
    16141 caaattggat atggatattc acctatttag caatcattct actaattgta ggatttttct
    16201 atgtattctt tattccacaa tcattacttg cctttggacc atatattcag gtaagcaatt
    16261 gggtatttat tatattttca tttatagtta tgcctattgg attacgcatt gaaagaaata
    16321 tttcgaaaaa acattctcat acatttttat aaacaaaaag cttattacat catatgctaa
    16381 tttggtactg ttaatgataa aatcactaaa ttacagttac ctaaaaacat attttgcaat
    16441 aagcttttta ttaattaatc aataaagcat ctataaagaa ccata
//
LOCUS       DAKSQE010000058        16448 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.058, whole genome shotgun sequence.
ACCESSION   DAKSQE010000058 DAKSQE010000000
VERSION     DAKSQE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16448
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.058"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            6..1577
                     /locus_tag="O0V33_001593"
     CDS             6..1577
                     /locus_tag="O0V33_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HCX8976223.1"
                     /translation="MIILTSVIGIVGRYMSRQRLLKSMETLWQTISPLESFIRPNSHF
                     DYEYKLYKDKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMKLYACLRGMLSITN
                     KSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRHNILFLCPFLPVISFAV
                     IFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLKQGYTISKIKHAPQ
                     PEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLFHSIQKSYTTHINE
                     LKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDSNDGIVFSELTHPLIADAVANDFS
                     LSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPGIVFTSMANADDVL
                     SGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAASESVLSFLHEKSNF
                     RVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKANTRNAIELLKITSF
                     PAKIYERAKDNVSNG"
     gene            complement(1891..2916)
                     /gene="tsaD"
                     /locus_tag="O0V33_001594"
     CDS             complement(1891..2916)
                     /gene="tsaD"
                     /locus_tag="O0V33_001594"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCX8976224.1"
                     /translation="MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITTTINEALVDADVSMEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLTFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(2909..3373)
                     /gene="rimI"
                     /locus_tag="O0V33_001595"
     CDS             complement(2909..3373)
                     /gene="rimI"
                     /locus_tag="O0V33_001595"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCX8976225.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDIERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            complement(3346..4008)
                     /gene="tsaB"
                     /locus_tag="O0V33_001596"
     CDS             complement(3346..4008)
                     /gene="tsaB"
                     /locus_tag="O0V33_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCX8976226.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIDALRAFLEDLNQPFIY
                     IGKDTVKLQDDLQGDVIAQLPNASVMYQLIDKPSDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            complement(3989..4450)
                     /gene="tsaE"
                     /locus_tag="O0V33_001597"
     CDS             complement(3989..4450)
                     /gene="tsaE"
                     /locus_tag="O0V33_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCX8976227.1"
                     /translation="MIKINNLDEMNQFAMFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENSRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            4488..4643
                     /locus_tag="O0V33_001598"
     CDS             4488..4643
                     /locus_tag="O0V33_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976228.1"
                     /translation="MIIHCSIVSNFIFNLYIVTNFNLNFLSLKQIISSKLIVRKFKIF
                     VDILKQI"
     gene            4961..6649
                     /gene="ilvD"
                     /locus_tag="O0V33_001599"
     CDS             4961..6649
                     /gene="ilvD"
                     /locus_tag="O0V33_001599"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCX8976229.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLALPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHSLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLKVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            6677..8446
                     /gene="ilvB"
                     /locus_tag="O0V33_001600"
     CDS             6677..8446
                     /gene="ilvB"
                     /locus_tag="O0V33_001600"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HCX8976230.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDTFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVKTIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDTAFAYQGPALIEVR
                     ISPTEAVNPMVPSGKSNHEMEGL"
     gene            8446..8700
                     /locus_tag="O0V33_001601"
     CDS             8446..8700
                     /locus_tag="O0V33_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCX8976231.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            8837..9841
                     /gene="ilvC"
                     /locus_tag="O0V33_001602"
                     /pseudo
     CDS             8837..9841
                     /gene="ilvC"
                     /locus_tag="O0V33_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500766.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
     gene            9871..11400
                     /locus_tag="O0V33_001603"
     CDS             9871..11400
                     /locus_tag="O0V33_001603"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCX8976232.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYIRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLYGK
                     HAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVIKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            11403..12449
                     /gene="leuB"
                     /locus_tag="O0V33_001604"
     CDS             11403..12449
                     /gene="leuB"
                     /locus_tag="O0V33_001604"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCX8976233.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFAN
                     IRPTTVVKGASSLSPLKAERVEGTDLVIVRELTSGIYFGEPRYFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYNMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            12463..13833
                     /gene="leuC"
                     /locus_tag="O0V33_001605"
     CDS             12463..13833
                     /gene="leuC"
                     /locus_tag="O0V33_001605"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCX8976234.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSNDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            13834..14406
                     /gene="leuD"
                     /locus_tag="O0V33_001606"
     CDS             13834..14406
                     /gene="leuD"
                     /locus_tag="O0V33_001606"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCX8976235.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKNAREHLAKYVEIEVDLPNQTVSSPDKSFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            14421..15689
                     /gene="ilvA"
                     /locus_tag="O0V33_001607"
     CDS             14421..15689
                     /gene="ilvA"
                     /locus_tag="O0V33_001607"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HCX8976236.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKSYSPNTKIIGVEPSGA
                     SSMYESVVVNNQVITLPNIDKFVDGASVARVGDITFEIAKKNVDNYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQRVNHFDPSNIYINENKMLYSLLI"
     gene            complement(15766..16182)
                     /locus_tag="O0V33_001608"
     CDS             complement(15766..16182)
                     /locus_tag="O0V33_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976237.1"
                     /translation="MKIFIGTMLTFLFVCSLIVCGFFAYKIYFPTDAENKSEIPNHMQ
                     ENKHEKENTHNINNNGSEANENFNNNKNRIENIEDKQKAYALMSIPRHNLPESEKAIY
                     DKVISTAIQNINNGVGNEEDFRLKEIYDGIQSVQPQ"
BASE COUNT         5502 a         2837 c         2882 g         5227 t
ORIGIN      
        1 tagcaattat tatacttaca tcggtaatag gaattgttgg acgatacatg agtcgtcaac
       61 gtctattaaa atctatggaa acattatggc aaacgatttc tccattagag tcttttatca
      121 gaccgaactc acatttcgac tatgagtata agctctacaa ggataaattt gaatcacatt
      181 ctttagttga tgataaaact tggtccgatt taaatatgaa tgcaatcttt cataagatga
      241 attataattt aacagctatt ggcgaaatga aactatatgc ctgtttacgt ggaatgcttt
      301 caattacgaa caaatcatta cttagtttat ttaatgataa tgctgaattt agaaaaaacg
      361 taacatatca tttagctttg attggtaaaa ctgtttatcc aacatttcca gaccaaatta
      421 caccggtaaa acgtcataat atattgtttc tatgcccgtt tttaccagtt atctcatttg
      481 cagttatatt tattaattca caagttggta ttttattatt tcttatgagt tgcctattta
      541 atatcatttt atctgcaact ttaaagcgta catatgagga tgacttaaag tctatatttt
      601 atgcatccaa tgttttaaaa caaggctata ctatctcaaa aattaaacat gctccacaac
      661 ccgaagtcaa ttttaagcaa tttagaacag cacgtcattt aacgagtgtt ttagctgaag
      721 ttaatgatga agatatcgga gctatggtaa ttaaactagt taaacttatt ttcatgcttg
      781 attatgtttt attccattca attcaaaaaa gctatacaac acatataaat gagctaaaaa
      841 attgtttcga ttacatagct gaattagata atcattatgc attagcaatg tatcgtagaa
      901 cgctagaatg ctataccgaa ccacaaattg atgattcaaa tgatggcata gtattctccg
      961 agttaacaca tccactcata gcagatgcag tagccaatga tttctcacta tcacaaaata
     1021 tactgttaac tggatctaat gcttcgggta aatcaacatt tatgaaatcc atagcaataa
     1081 acatcattct tgcatcggct atacaaacag ttaccgcaag caaatttgta tatcaacctg
     1141 gaattgtgtt cacgtcaatg gcaaatgccg atgatgtgtt atcaggcgat agttatttta
     1201 tggctgaatt aaaatctatt aagcgtattg ttgaaattcc tgataatcaa aaaatatact
     1261 gctttatcga tgaaattttt aaaggtacca acacaactga acgaattgcc gcttcagaat
     1321 cagtactatc atttttacat gaaaaatcta actttagagt tattgcagca acacatgata
     1381 ttgagttagc tgaactctta aaacaacgtt atgaaaatta tcatttcaat gaggtaatag
     1441 aaaataataa catacatttt gattacaaaa ttaagcctgg caaagcaaat acacgtaatg
     1501 ccatcgaatt attaaaaatc acttcatttc cagcaaaaat atatgaacga gcaaaagata
     1561 atgtttccaa tggttagcat gaatcttgaa catcaatcaa tgttcaagtc gcaacattgt
     1621 tatattggca gtctttgacc aaatgaaaat gcgcttgtaa caagtttttt caatactagt
     1681 ccgcggcccc aacacagaag ctggcggaaa gtcagctttc aatcgtgtac aagttggcgg
     1741 ggccccaaca taataaatca ttagtggttc tttatctttt ctgtctcact cccctaattg
     1801 caatataaaa acgtcagcta tcacatgaca gaagacaatg aacagtttca ataatattca
     1861 tcgtaatcat gttaataact gacgtttatt ttattctgca gaatactctt ctaaatctat
     1921 attgctgtgc ccatttaatg ctaaatcagc aaatcgacct tgctgataca aatagtggcc
     1981 ggcaacgcct atcattgcag cattatctgt gcataattta ggacttggga tagttaattg
     2041 aatgtcattg actttgcatt gatccgctaa agattgacgt aaccctttat tactcgccac
     2101 gccaccagca acaattaatc gctgaacacc atattcttta caagcttgaa tagctttaaa
     2161 cgtgagaacc tctacaacac tgttttgaaa gctcgttgct acgttagctt caatgattgg
     2221 aatatttttt tgtcgttgat tgtgaagttg attgattacg gcacttttca acccactaaa
     2281 actaaaatca taactatctt tatccaacca aacacgaggg aatgaataag tatcttcacc
     2341 ttcagcagcc aaccggtcaa cttgtggacc acctggataa tttaaaccaa ttgttcgtgc
     2401 cactttatca taagcctcac ctactgcgtc atctcgtgtt tcaccaatga cttcaaatgt
     2461 taaatgatct ttcatataaa ctaattcagt atgtccacct gaaacaataa gtgcaattag
     2521 cgggaatgtt aatggctctt ctatgtgatt agcatatata tgtcctgcaa tatgatgaac
     2581 aggaataagc ggcttatcgt aagcaaatgc caatgctttg gctgcattaa cacctattag
     2641 taacgcacca attagtccag ggccttctgt aaccgctatg gcatcaatat cttccattga
     2701 tacgtcggca tccactagag cctcgtttat tgttgttgtt ataccttcaa cgtgatgtct
     2761 acttgccact tcgggaacga caccgccaaa tcgtttatga ctttcaatct gacttaaaac
     2821 tgtatttgat aaaatatctc tgccattttt tataacacta acgcttgttt catcacaact
     2881 tgtttcaaca gctagtatta atatatcttt agtcatttaa attcacccac ataaccattg
     2941 cgtcctcacc ttcaccataa taatttttac gtttaccacc atattgaaat cctaaatttt
     3001 catatacatg ttgtgccact ttattattaa ctcttacttc taaactcatc acatcacaag
     3061 tgtgacttgc atagtttatt ccgtatttta aaagcatttg acctaaacca tatcctctat
     3121 aattatcatc gattgcaact gtcgtaattt gagcttgatc gataacaatc cataaaccta
     3181 aataaccaat aatttgttgt tcaaattcta agacaaaata tttcgcaaag ttattttgct
     3241 ctatttcatg ataaaatgcg tcaattgtcc aagaactatc attgaaactc cgacgctcaa
     3301 tatcaaagac ttgtggcaca tcttctttgg tcatctctct aatgtttaat tgttcttttg
     3361 actgttgatc caatttcgtt ccgcctcagc taatttatgg tatttaggag taaatgtatg
     3421 tacgtctgaa ggtttatcta tcaattgata catgactgat gcatttggta gctgcgcaat
     3481 cacatcacct tgtaaatcat cttgtaattt tactgtatct ttcccaatat aaataaatgg
     3541 ttggtttaaa tcttctaaaa aagctcgcaa tgcatctatc gacatatatt gatcttctaa
     3601 aatagtcact aattgaccat tttgccattg gaatatgcct gtataaactg cttgtcgtct
     3661 tgcatcaaac acaggaacca ataatttatc agtatgatcg attgttgctg ccaatgcctt
     3721 taatgatgaa acaccatata atttaacatc taacgcatac gctaatgttt tagcaacagt
     3781 aacaccgata cgtaagccag tatatgaacc aggaccttca gcaacaataa tcgcatctaa
     3841 ttgctgttta gctattttgc tctgctcaaa tagctgacta atcgcaggca ttaattgcac
     3901 tgaatgattt tgtttagaat ccgtagttat ttcagctaaa acttcatcgt tttgcatcaa
     3961 tgctactgat aatggttgat tcgatgtatc aatgagcagc gaattcatgg ataattgcct
     4021 ccttaatttg ttcataatgt tctccttgcg cgaacaactc aatttgtctt gaattttcag
     4081 atattgttga aatgttaata gataaatgcg tcgctgggag taaatctttt ataaattgac
     4141 tccattcaat aacagtaatt gcctgatctt cgaaaaattc atcaaaccct aaatcttcat
     4201 cagaatcttc taagcgataa caatccatat gatgcaattt taaatttttt cccctatatg
     4261 atttgatgat gttaaatgtc ggggaattaa tcgtacgtct tacaccaaga gcttttccta
     4321 taaattgtgt taacgttgtt ttacctgctc ctaaatctcc gttaagtaaa atcaaatcac
     4381 cacttttcaa ttgctcaact aaaaacatag caaattgatt catttcatct aaattattta
     4441 tctttatcaa tgttgattct cctatattat gcttttcatt cataaaaatg attatccatt
     4501 gttcaatcgt atctaacttt atatttaacc tttatattgt aacaaatttc aacttaaatt
     4561 tcttatcttt gaaacagatt atctcttcaa agttaattgt aagaaaattt aaaatatttg
     4621 ttgacatact aaagcagata tagtaaatta aatttatcaa atttttagac aattctaact
     4681 attaaagtgg tatataccat tcacggaagg agtataataa aatgcttaat caatatactg
     4741 aacatcaacc gacaacttca aatattatta ttttattata ctctttagga ctcgaacgtt
     4801 agtaaatatt tactaaacgc tttaagtcct atttctgttt gaatgggact tgtaaacgtc
     4861 ccaataatat tgggacgttt ttttatgttt tatctttcaa ttacttattt ttattactat
     4921 aaaacatgat taatcattaa aatttacggg ggaatttact atgcgaagcg acatgatcaa
     4981 aaaaggagat caccaagcac cagcaagaag tcttttacat gccacgggcg cgcttaaaag
     5041 tccaactgat atgaacaaac catttgtagc tatttgtaac tcttatattg atatcgttcc
     5101 aggacatgtt catttaagag agcttgcaga tatagctaaa gaagcaatta gagaagccgg
     5161 tgccattcca tttgaattca atacaattgg tgttgatgat ggaatagcta tgggacatat
     5221 cggaatgcga tattctctac catcacgtga aattattgca gatgcagctg aaactgtaat
     5281 taacgctcat tggtttgacg gcgtatttta cattcctaat tgtgacaaga ttacacccgg
     5341 tatgatttta gcagccatga ggacaaacgt accagctatc ttttgctctg gtggaccaat
     5401 gaaagctggc ttatctgcac atggtaaagc attaacactt tcatcaatgt ttgaagcagt
     5461 cggcgctttt aaagaaggat cgatttctaa agaagaattt ttagatatgg aacaaaatgc
     5521 ctgccctact tgtggttcat gtgctgggat gtttactgca aattcaatga actgtttgat
     5581 ggaagtttta ggtctagcat taccatacaa cggtactgca cttgcagtca gtgatcagcg
     5641 acgcgaaatg attcgccaag cagcttttaa attagttgaa aatattaaaa atgatttaaa
     5701 accacgtgat atcgttactc gcgaagcaat tgatgatgca tttgcacttg atatggctat
     5761 gggtggttca acaaacacgg tactgcatac gttagccatt gccaatgaag ctggtattga
     5821 ttatgactta gagcgcatta atgctattgc caaacgcacg ccatatttat caaaaatagc
     5881 acctagttca tcgtattcaa tgcatgatgt gcatgaagct ggtggcgtcc cagcaattat
     5941 taatgaattg atgaagaaag atggcacgtt acacccagat agaatcacag ttactggcaa
     6001 aacgttacgt gaaaataacg aaggcaaaga aattaagaac tttgatgtca ttcactctct
     6061 tgatgcacca tatgatgcac aaggcggttt atctatctta tttggtaata tcgcccctaa
     6121 aggcgcagtt attaaagttg gcggcgttga tccatctatc aaaacattta ctgggaaagc
     6181 aatttgtttc aattcgcatg atgaagctgt tgaagcaata gacaatcgta ccgttcgtgc
     6241 aggccacgtc gttgtcatta gatatgaagg acctaaaggt ggaccaggta tgcctgaaat
     6301 gttagcacct acttcctcta ttgttggtcg tggcttaggt aaagatgttg cattaattac
     6361 tgatggtcgt ttttccggtg ccacaagagg tattgcagtt ggtcatattt cccctgaagc
     6421 tgcatctggt ggaccaattg ccttaattga agatggagat gagattacta ttgatttaac
     6481 aaatcgtaca ttaaaggtaa accagcctga agatgttctg gcgcgtcgcc gagaatcttt
     6541 aacaccattt aaagcgaaag taaaaacagg ttatctagct cgttatactg ccctagtaac
     6601 tagcgcaaat acaggtggcg tcatgcaagt ccctgagaat ttaatttaat ttatttttat
     6661 attggagatg gttaaaatgt ctaaaactca acatgaagta aaccaaaata ttgacccttt
     6721 aaaaatggct gaatcacttg aacctgaaca actaaatgaa aaaactttaa atgacatgcg
     6781 ttcaggatca gaagtgctag tagaagctct acttaaagaa aatgtggatt atttattcgg
     6841 ttatcctggt ggtgccgtac tacctttata cgacacattt tatgatggta aaatcaaaca
     6901 tattttagca agacacgaac aaggtgctgt tcatgctgca gaaggttatg cacgtgtatc
     6961 tggtaaaaca ggcgtcgttg tagttacaag cggtccaggt gcaactaatg taatgacagg
     7021 tattacggat gcacattgcg actctttacc actagttgta ttcactggac aagttgctac
     7081 accaggcatt ggtaaagatg cattccaaga agcggatatt ctatctatga cttcaccaat
     7141 tacaaaacaa aattatcaag tgaaacgtgt tgaagatatc cctaaaatcg tacacgaagc
     7201 tttccatgta gctaattctg gacgcaaagg tcctgtagtg attgattttc caaaagatat
     7261 gggtgtttta gctacaaatg tggatttatg cgacgaaatc aatattccag gttatgaagt
     7321 tgttacagaa ccagaaaata aagacattga cactttcatc tcacttttaa aagaagcgaa
     7381 aaagcctgtc gtattagccg gcgcaggtat taatcaatca aaatcaaatc aattattaac
     7441 acagtttgtt aataaacatc aaatcccaac agttactact ttactaggtc taggtgctgt
     7501 accttatgag gatacactat ttttaggtat gggaggtatg catggttctt atgctagtaa
     7561 catggcatta actgagtgtg atttactcat taatttaggt agccgcttcg atgatagatt
     7621 agcaagcaaa cctgatacct ttgcacctaa cgccaaaatt gtacatgtag atattgatcc
     7681 ttcagaaatc aataaagtta ttcatgtaga tttaggtatt attgcagact gtaaaagatt
     7741 tttagaatgt ttaaatgata aaaatgttaa gactatagaa cacagtgact gggttaaaca
     7801 ttgtcaaaat aataagcaga aacatccatt taaacttggt gaagaagatc aagtattttg
     7861 taagccacaa caaacaatcg aatatatcgg caaaattaca aatggtgaag caattgttac
     7921 tacagacgtg ggacaacatc aaatgtgggc agctcaattt tatccattta aaaatcacgg
     7981 acaatgggtt acaagcggtg gtttaggaac aatgggattc ggtattcctt cgtcaattgg
     8041 tgccaaatta gctaatcctg ataaaacagt cgtatgcttc gtcggtgacg gtggtttcca
     8101 aatgacaaac caagaaatgg cactattacc cgaatatggt ttagatgtca aaatcgtact
     8161 aatcaataat ggaacattag gtatggttaa acaatggcaa gataagttct ttaatcaacg
     8221 cttctcacac tcagtattta atggtcaacc tgattttatg aaaatggcag aagcatatgg
     8281 cgtcaaaggt ttcttaatcg ataagccaga acaactggaa gaacaattag atacagcgtt
     8341 tgcttatcaa gggccagctt taattgaggt tcgtatttcc cctactgaag ctgtaaaccc
     8401 aatggttcca agtggcaaat caaatcatga aatggagggc ttataatgac aagaattctt
     8461 aaattacaag ttgcggatca agtcagcacg ctaaatcgaa ttacaagtgc ttttgttcgc
     8521 ctacaatata atatcgatac attacatgtt acacactctg aacaacctgg gatttctaac
     8581 atggaaattc aagtcgatat tcaagatgat acatcacttc atattttaat taaaaaatta
     8641 aaacaacaaa ttaatgtttt aacggttgaa tgctacgacc ttgttgataa cgaagcttaa
     8701 ttttaagaca aaggcaatga tgcgctaatt agttatagat atatcatagg ctgctagtta
     8761 acatctgcca ctattacaaa gctatatttc agaattttcg aaaaacaaaa catttaatta
     8821 tttggaggaa tttattatga caacagttta ttatgatcaa gatgtaaaaa cggacgcttt
     8881 acaaggcaaa aaaattgcag tagtaggtta tggatcacaa ggccacgcgc atgcacaaaa
     8941 cttaaaagac aatggatatg atgtagtcat cggtattcgc ccaggtcgct cttttgacaa
     9001 agctaaagaa gatggatttg atgtgttccc tgttgcagaa gcagttaagc aagctgatgt
     9061 aattatggtg ctattacctg atgaaattca aggtgatgta tacaaaaacg aaattgaacc
     9121 aaatttagaa aaacataatg cgcttgcatt tgctcatggc tttaacattc attttggtgt
     9181 tattcaacca ccagctgatg ttgatgtatt tttagtagct cctaaaggac cgggtcattt
     9241 agttagacgt acatttgttg aaggttctgc tgtaccatca ctatttggta ttcaacaaga
     9301 cgcttcaggt caagcacgta atattgcttt aagttacgca aaaggtattg gtgcaactcg
     9361 tgcaggtgtt attgaaacaa catttaaaga agaaactgag acagatttat ttggtgaaca
     9421 agcagtactt tgcggtggtg tatcgaaatt aattcaaagt ggctttgaaa cattagtata
     9481 agcaggttat caaccagaat tagcttattt tgaagtatta catgaaatga aattaatcgt
     9541 tgatttgatg tatgaaggcg gtatggaaaa tgtacgttac tcaatttcaa atactgctga
     9601 atttggtgac tatgtttcag gaccacgtgt tatcacacca gatgttaaag aaaatatgaa
     9661 agctgtatta actgatatcc aaaatggtaa cttcagtaat cgctttatcg aagacaataa
     9721 aaatggattc aaagaatttt ataaattacg cgaagaacaa catggtcatc aaattgaaaa
     9781 agttggtcgt gaattacgcg aaatgatgcc ttttataaaa tctaaaagca ttgaaaaata
     9841 agatagacct acaatgagga gttgttaaat atgagtagtc atattcaaat ttttgatacg
     9901 acactaagag acggtgaaca aacaccagga gtgaatttta cttttgatga acgcttgcgt
     9961 attgcattgc aattagaaaa atggggtgta gatgttattg aagctggatt tcctgcttca
    10021 agtacaggta gctttaaatc tgttcaagca attgcacaaa cattaacaac aacggctgta
    10081 tgtggtttag ctagatgtaa aaaatctgac atcgatgctg tatatgaagc aacaaaagat
    10141 gcagcgaagc cggtcgtgca tgtttttata gcaacatcac ctattcatct tgaacataaa
    10201 cttaaaatgt ctcaagaaga cgttttagca tctattaaag aacatgtcac atacgcgaaa
    10261 caattatttg atgttgttca attttcacct gaagatgcaa cgcgtactga attaccattc
    10321 ttagtgaaat gtgtacaaac tgcagttgac gctggagcta cagttattaa tattcctgat
    10381 acagtcggct acagttacca tgatgaatat gcacatattt tcaaaacctt aacagaatct
    10441 gtaacatctt caaatgaaat tatttatagt gctcattgcc atgacgattt aggaatggct
    10501 gtttcaaata gtttagctgc gattgaaggc ggtgcgagac gaattgaagg cactgtaaat
    10561 ggtattggtg aacgagcagg taatgcagca cttgaagaag tcgcgcttgc actatacatt
    10621 cgaaatgatc attatggtgc tcaaactgcc cttaatctcg aagaaactaa aaaaacatcg
    10681 gatttaattt caagatatgc aggtattcga gtgcctagaa ataaagcaat tgttggccaa
    10741 aatgcattta gtcatgaatc aggtattcac caagatggcg tattaaaaca tcgtgaaaca
    10801 tatgaaatta tgacacctca acttgttggt gtaagcacga ctgaacttcc attaggaaaa
    10861 ttatatggta aacacgcctt ctcagagaag ttaaaagcat taggttatga cattgataaa
    10921 gaagcgcaaa tagatttatt taaacaattc aaggccattg cggacaaaaa gaaatctgtt
    10981 tcagatagag atattcatgc gattattcaa ggttctgagc atgagcatca agcactttat
    11041 aaattggaaa cactgcaact acaatatgtc tctagcggcc ttcaaagtgc tgttgttgtt
    11101 attaaagata aagagggtca tatttaccag gattcaagta ttggtactgg ttcaatcgta
    11161 gcaatttaca atgcagttga tcgtattttc caaaaagaaa cagaattaat tgattatcgt
    11221 attaattctg tcactgaagg tactgatgcc caagcagaag tacatgtaaa tttattgatt
    11281 gaaggtaaga ctgtcaatgg ctttggtatt gatcatgata ttttacaagc ctcatgtaag
    11341 gcatacgtag aagcacatgc taaatttgca gctgaaaatg ttgaaaaggt aggtaattaa
    11401 ttatgactta taacattgtt gccctacctg gtgatggaat cggtccagaa attttgaacg
    11461 gatctctatc attgcttgaa attataagta ataaatataa ttttaattat caaatagagc
    11521 accacgaatt tggtggtgcc tctattgata cattcggcga gcctttaact gagaaaacct
    11581 taaatgcgtg taaaagagca gatgctattt tactgggtgc aatcggtgga cctaaatgga
    11641 cagatcctaa caatcgacca gaacaaggat tattaaaatt acgtaaatca ttaaatttat
    11701 ttgcaaatat acgtcccact accgttgtca aaggcgctag ttctttatca cctttaaagg
    11761 cagaacgcgt tgaaggcaca gatttagtta tagtccgtga attgacaagt ggtatttatt
    11821 ttggagaacc tagatatttt aataatcacg aggccttaga ttctcttact tatacaagag
    11881 aagaaataga acgcattgtt cacgtagcat ttaaattggc cgcttcaaga cgaggaaaac
    11941 taacatcagt tgataaagaa aatgtattag cttctagtaa attgtggcgc aaagtcgtaa
    12001 atgaagtgag tcaattatat ccagaagtaa cagtgaatca cttacttgtt gatgcttgta
    12061 gtatgcattt aatcacaaat ccaaaacaat ttgacgtcat cgtttgcgaa aacttatttg
    12121 gcgatatttt aagtgatgaa gcttcagtga ttcctggttc acttggttta tcaccttctg
    12181 ctagttttag taacgatggt ccaagattgt atgagcctat tcatggatca gcaccagata
    12241 ttgcaggtaa aaacgttgcc aatccatttg gaatgattct atctttagcg atgtgtttac
    12301 gtgaaagctt aaatcaacca gatgctgcag atgaattaga acaacatatt tataacatga
    12361 tagaacatgg gcaaacgaca gcagatttag gcggcaaact gaatactact gatattttcg
    12421 aaattctatc tcaaaaattg aatcactaag ggggagatgt aaatgggtca aacattattt
    12481 gacaaggtgt ggaacagaca tgtgttatac ggaaaattag gcgaaccgca actattatac
    12541 attgatttac accttataca tgaagttact tctcctcaag catttgaagg acttaggctt
    12601 caaaacagaa aattaagacg cccagattta acatttgcaa cactcgatca caatgttcct
    12661 actattgata ttttcaatat taaagatgaa attgcaaaca aacaaatcac aacattacaa
    12721 aaaaacgcca tagattttgg ggtgcatatt tttgatatgg gttctgatga acaaggtatt
    12781 gttcacatgg tgggacctga gacaggactt acacagcctg gcaaaacaat cgtttgtggt
    12841 gactctcaca cagcaacaca tggagcattt ggtgctattg catttggaat tggaacaagt
    12901 gaagttgaac atgttttcgc aactcaaacg ctatggcaaa caaaacccaa aaacttaaaa
    12961 atcgatatta atggcacctt accaacaggc gtctatgcta aggacattat tctgcattta
    13021 attaaaacgt atggtgttga ttttggtaca ggctatgctt tggaatttac tggtgaaaca
    13081 attaaaaacc tttcaatgga cggtcgaatg actatttgta acatggctat cgaaggtggt
    13141 gccaaatacg gcataatcca acctgatgat ataacatttg aatatgttaa aggtcgacca
    13201 tttgccgata acttcgctaa atcagttgat aagtggcgtg agctatattc taatgacgac
    13261 gcgatatttg atcgtgtaat tgaacttgat gtttcaacat tagaaccaca agtgacatgg
    13321 ggaactaatc ctgaaatggg tgttaatttc agtgaaccat tccctgaaat caatgatatc
    13381 aacgatcaac gtgcgtatga ttatatgggg ttagagccag gacaaaaagc tgaagatatc
    13441 gacttagggt atgttttcct cggttcatgt acaaatgcta gactatcaga tttgattgaa
    13501 gctagtcata ttgttaaagg aaataaagtt cacccaaata ttacagctat cgtcgtacca
    13561 ggttctcgta cagtaaaaaa agaagctgaa aaattaggtc tagatactat ctttaaaaat
    13621 gcaggatttg aatggcgtga gccaggatgt tcaatgtgtt taggcatgaa tcctgaccaa
    13681 gtacctgagg gcgtacattg tgcatctaca agtaatcgaa actttgaagg acgacaaggc
    13741 aaaggtgcaa gaacacattt agtatcccct gctatggcag cagcagcagc tattcatggt
    13801 aaatttgtgg acgtaagaaa ggtggttgtt taaatggcag caatcaaacc tattacaaca
    13861 tataaaggta aaatagtccc tcttttcaac gacaatatcg atacagacca aatcattcct
    13921 aaagtacact taaagcgtat ttcaaaaagt ggctttggtc catttgcttt tgatgaatgg
    13981 cggtacttac ctgatggttc agataatcct gatttcaatc caaataagcc tcaatataaa
    14041 ggtgcatcca tattaattac tggagataat tttggatgtg gttcaagtcg tgaacatgca
    14101 gcttgggcac ttaaggacta tggctttcat attataattg caggaagttt cagtgacatt
    14161 ttttatatga attgtacaaa aaatgcaatg ttaccaattg ttttagaaaa aaatgcacgt
    14221 gaacatcttg ctaaatatgt tgaaattgag gtcgatttac caaatcaaac tgtgtcatca
    14281 ccagacaaga gtttccattt tgaaattgat gaaacttgga agaataaact tgtaaatggc
    14341 ttagatgaca ttgcaatcac cctacaatat gaatcattaa tagaaaaata tgaaaaatca
    14401 ctttaaggga gttgaatatt atgacagtca aaacaacagt ttctacgaaa gatatcgatg
    14461 aagcattttt aagacttaaa gatattgtca aagaaacacc tttacaatta gaccattact
    14521 tatctcaaaa gtatgattgt aaagtttatt taaaacgaga agatttacaa tgggtacgtt
    14581 cttttaaatt aagaggtgct tacaatgcta tttctgtttt atcagatgaa gctaaaagta
    14641 aaggtattac atgtgcgagt gcaggtaatc atgctcaagg tgttgcctat acagctaaaa
    14701 aacttaattt aaacgctgtt atctttatgc cagtcactac acctttacaa aaggtaaatc
    14761 aagtaaaatt ctttggaaat agtaacgttg aagttgtact aactggtgat acatttgatc
    14821 actgtttagc tgaagcttta acttatacaa gtgaacatca aatgaacttt atagatccat
    14881 tcaataatgt tcatacaatt tctggacaag gtacgcttgc taaagaaatg ctagaacaag
    14941 caaagtctga caatgttaac tttgattatc tatttgccgc aattggtggt ggtggtttaa
    15001 tttcaggtat tagtacctat tttaaatcct attctcccaa cacaaaaatt atcggagttg
    15061 aaccttcagg tgctagtagt atgtacgaat ctgttgtcgt aaataatcaa gttatcacat
    15121 tacctaatat cgataaattt gtcgatggag cttctgtagc cagagttggc gatattactt
    15181 ttgaaattgc caaaaaaaat gtagataact acgtacaagt agatgaaggt gctgtatgtt
    15241 caacaatttt ggatatgtat tcaaaacaag caatagtagc agaacccgct ggcgctttaa
    15301 gtgtaagtgc gcttgaaaac tataaagatc atattaaagg taaaacagtg gtttgtgtca
    15361 ttagtggtgg taataatgat attaatcgaa tgaaagaaat tgaagaacgt tcattactat
    15421 atgaagaaat gaagcattac tttatcttaa atttccctca acgtccaggt gcattgagag
    15481 aatttgtaaa tgacgtatta ggacctcaag acgatattac taaatttgaa tacttaaaaa
    15541 aatcttctca aaatacaggt actgtcatta ttggtattca acttaaagat catgatgatt
    15601 taatacaact caaacaacgt gtaaatcatt tcgatccttc caatatttat attaatgaaa
    15661 ataagatgtt atattcattg ttaatttaac acatattaag aaaagcagtc ataaattgat
    15721 ttctaattga aatcatctta tgactgcttt ttattatgct ttacattatt gaggttgcac
    15781 gctttgtata ccatcgtata tttctttgag cctaaagtct tcttcattcc ccaccccatt
    15841 atttatattt tgaatggcag ttgaaattac tttgtcataa atagccttct cactttctgg
    15901 taaattatgt cgtggtatac tcatcaaagc atatgctttt tgtttatctt caatattttc
    15961 tatacgattc ttattattat taaaattctc attagcttca ctaccattat tattaatatt
    16021 atgcgtgttt tctttttcat gtttgttttc ttgcatatga ttcgggattt ctgacttatt
    16081 ttcagcatct gtaggaaaat atattttata agcaaaaaat ccacacacaa tcaatgagca
    16141 tacaaataaa aaagttaaca tcgtaccaat aaatattttc aaatccgttc tcctttcaac
    16201 cattcattct tacatgtata tttagtatta aaaaatcctt atagtctcaa cttgcctggc
    16261 aacgttctac tctagcggaa cgtaagttgg ctaccatcgt cgcttactca tttagctcta
    16321 ctaaactcgt tgcgctcttt tctcgtttcg tcagattcaa acgttttcac ttcgccaagc
    16381 catttttctt tgtgttaact tttattttga cgttttagac ataaaaaaag agaccttgcg
    16441 gtctcaac
//
LOCUS       DAKSQE010000059        16335 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.059, whole genome shotgun sequence.
ACCESSION   DAKSQE010000059 DAKSQE010000000
VERSION     DAKSQE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16335
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.059"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(125..1111)
                     /locus_tag="O0V33_001609"
     CDS             complement(125..1111)
                     /locus_tag="O0V33_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCX8976238.1"
                     /translation="MNGQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQEIILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGSFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
     gene            1811..2569
                     /gene="map"
                     /locus_tag="O0V33_001610"
     CDS             1811..2569
                     /gene="map"
                     /locus_tag="O0V33_001610"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCX8976239.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
     gene            2786..3172
                     /locus_tag="O0V33_001611"
     CDS             2786..3172
                     /locus_tag="O0V33_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976240.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMHYLITK
                     KEQLSKLIDSLMLTLENKYIDIVEAFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            3187..3888
                     /locus_tag="O0V33_001612"
     CDS             3187..3888
                     /locus_tag="O0V33_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCX8976241.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            3885..4928
                     /locus_tag="O0V33_001613"
     CDS             3885..4928
                     /locus_tag="O0V33_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCX8976242.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            4918..5547
                     /gene="vraR"
                     /locus_tag="O0V33_001614"
     CDS             4918..5547
                     /gene="vraR"
                     /locus_tag="O0V33_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HCX8976243.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMEDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(5660..6877)
                     /locus_tag="O0V33_001615"
     CDS             complement(5660..6877)
                     /locus_tag="O0V33_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCX8976244.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQF
                     QSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMF
                     PMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILA
                     IWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVI
                     SHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIVFIVLYSVAPNVKTKLKSVLPGAVFT
                     SIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQR
                     SVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDTNATHQRTYHVDEHPSDTYPEDDK
                     NIKDKIVDKLKKD"
     gene            complement(7155..7430)
                     /locus_tag="O0V33_001616"
     CDS             complement(7155..7430)
                     /locus_tag="O0V33_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCX8976245.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            complement(7437..7901)
                     /locus_tag="O0V33_001617"
     CDS             complement(7437..7901)
                     /locus_tag="O0V33_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCX8976246.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIQDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            8048..8248
                     /locus_tag="O0V33_001618"
     CDS             8048..8248
                     /locus_tag="O0V33_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HCX8976247.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHD"
     gene            8251..9507
                     /locus_tag="O0V33_001619"
     CDS             8251..9507
                     /locus_tag="O0V33_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCX8976248.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYRCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGKEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAEKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            9597..10127
                     /locus_tag="O0V33_001620"
     CDS             9597..10127
                     /locus_tag="O0V33_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCX8976249.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(10387..11538)
                     /gene="yfkAB"
                     /locus_tag="O0V33_001621"
     CDS             complement(10387..11538)
                     /gene="yfkAB"
                     /locus_tag="O0V33_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HCX8976250.1"
                     /translation="MNSVKLKQPVSIHNDPWEAYNDVKEHGQLTLSNIEFTTTNLCNM
                     RCSHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPL
                     LKYAHNRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQP
                     PLKAKLKLYEQMISNACTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEI
                     HPMYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLS
                     RLRTAKNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLS
                     SDLAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
     gene            11762..11923
                     /locus_tag="O0V33_001622"
     CDS             11762..11923
                     /locus_tag="O0V33_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976251.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            12053..12568
                     /locus_tag="O0V33_001623"
     CDS             12053..12568
                     /locus_tag="O0V33_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCX8976252.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVF
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
     gene            12909..13718
                     /gene="sgtB"
                     /locus_tag="O0V33_001624"
     CDS             12909..13718
                     /gene="sgtB"
                     /locus_tag="O0V33_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HCX8976253.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
     gene            13979..14797
                     /gene="recX"
                     /locus_tag="O0V33_001625"
     CDS             13979..14797
                     /gene="recX"
                     /locus_tag="O0V33_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCX8976254.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQHLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEMFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            14775..15089
                     /locus_tag="O0V33_001626"
     CDS             14775..15089
                     /locus_tag="O0V33_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HCX8976255.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            15104..>16335
                     /locus_tag="O0V33_001627"
     CDS             15104..>16335
                     /locus_tag="O0V33_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8976256.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAQDIDLNNISLHI
                     YQGEALGIIGEPESSKAMVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINNHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDLQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPFMKSNYS"
BASE COUNT         5915 a         2354 c         2854 g         5212 t
ORIGIN      
        1 tgtggctata tttgagtctg aatatatatg cttgtaattt taaaaaggga catgctatat
       61 acgataaaat tactgataaa gtaagaatat atctaatgat tccaattagc ataaggcact
      121 taaattatgc atatctgctg tctagcagtc gataaatcat taaaacttca tacagtatat
      181 gacttttaat ttgattttca ccactaattt caagtgcttt tattgtcgaa cgtaaagttt
      241 ctacagaatc atcttctctc ttaaatgaac catcataaaa aatatctttg atgctactac
      301 taatttttag caatgccatt ttttcgtcac ctgaaaagtt aacacgagta tttttaggca
      361 agtaaatgat atttgataaa tgagtgataa acaaacgatt cgtatatgca cgtttagtta
      421 attgattgag caatttccaa tcacattctt ttttcttatg atagcttaat tcatcacgtt
      481 gataacttat taacgtttca acttgattgt ttaaattgaa aatattttta tatgcttttt
      541 cgcttttatc agattgcagt cttgataaga taatttcttg gcagcgattg taaaataatt
      601 tatacatcaa ggcatctgtc ttacttaatt tttcttcgac ctgaccataa tacttaggtg
      661 gaaacaccat gaagttaatt aaacctgatg tcacgagtcc aataatcgct gtcaatgttc
      721 gagacaaaaa gttgaatatg taggcatcat gaatacctgg aatcatagct aatgatgtta
      781 gtacagcaac attcgtacca acttgcaatt tgagttttgt acagaataaa atcgtgaacg
      841 tcgcactcaa tgcatatgta aaaggtgatt gatcgccgaa taaatatgta aataatactg
      901 caaagcctgc accaattacc gtagcaggta atctacgata accttttata agtgatgcct
      961 tggcagttgg ttcaattgtg actacagctg ttaaaatggc atagatgggt gttaaatcta
     1021 gtgccataca aaagacagcc gttaaaaaaa tggcaatacc agttttaatt gttctggcac
     1081 caattaaatg tttataccat tgaccgttca ttttttaacc tctaatcatc gtaaaatctt
     1141 agcgagcgct ttataataat agtatcgtac attggaaaag ttcatgtatg taaaacattt
     1201 gaaataatca tgcttatgca ttactttgac tttcatatac attaatcaaa gctctgggct
     1261 ccacaatgtt atattgttag tagttgactg aaataaaatg cacttgtaac aagcattttt
     1321 caattctagt ccggggcccc aacatagaga aattggaaca ccaatttctg cagacaatgc
     1381 aagttgggga ggggccccaa cacagaagct gacgaaaagt cagcttacga taatgtgcag
     1441 gttggcgggg ccccaacata gagaaattgg atctacaatt tctacaggca atgcaagttg
     1501 gggtgtgggc cccaacacag agaatttcga aaaaagaaat tctacaggca atgcaggttg
     1561 gggtgtaggc cccaacacag agaaattgga acaccaattt ctataggcaa tgcaagttgg
     1621 cggggcccca acacagagaa tttcaaaaaa gaaattctac aggcaatgca agttggggac
     1681 gattaagaaa taattttctt tatcacacta tgtcccactc actttccaaa atactaaagt
     1741 aacatcttta gtatatcaaa gaatttttgc tataataagt tataattata taaaaaagga
     1801 acgggataaa atgattgtaa aaacagaaga agaattacaa gcgttaaaag aaattggata
     1861 catatgcgct aaagtgcgca atacaatgca agctgcaacc aaaccaggta tcactacgaa
     1921 agagcttgat aatattgcga aagagttatt tgaagaatac ggcgctattt cagcgccaat
     1981 tcatgatgaa aattttcctg gtcaaacgtg tattagtgtt aatgaagagg tggcacatgg
     2041 gattccaagt aagcgtgtca ttcgtgaagg agatttagta aatattgatg tatcggcatt
     2101 gaagaatggc tattatgcag atacaggcat ttcatttgtc gttggagaat cagacgatcc
     2161 aatgaaacaa aaagtatgtg acgtagcaac gatggcattt gagaatgcaa ttgcaaaagt
     2221 aaaaccgggt actaagttga gtaacattgg taaagcggtg cataatacag ctagacaaaa
     2281 tgatttgaaa gtcattaaaa acttaacagg tcatggtgtt ggtttatcat tacatgaagc
     2341 gccagcacat gtacttaatt actttgatcc aaaagacaaa acattattaa ctgaaggtat
     2401 ggtattagct attgaaccgt ttatctcatc aaatgcatca ttcgttacag aaggtaaaaa
     2461 tgaatgggct tttgaaacga gcgataaaag ttttgttgct caaattgagc atacagttat
     2521 cgtgactaag gatggtccga ttttaacgac aaagattgaa gaagaatagt tcaacatata
     2581 ctaagactaa agtatgaaca tcatttagtt ccggagccta ttcatattgg tttcggaact
     2641 gttttataat aattaagaac acaatcaatg cgtcatttca aaaatatgtt gtaataaagt
     2701 agtttataag caaacatatc atcgacatca acgaagatac atagcgcatt tggtacttta
     2761 aaacttatta taaaaggtga tagttatgaa ctatgttgaa cgttatattg aacagttttt
     2821 gagagcaaca gtaagaaata atatcaagca ctacctttta atgttagatg aaaaaatgaa
     2881 aaatttagat gattatatgc attatttaat tactaaaaaa gaacaactta gcaagttaat
     2941 tgacagtcta atgctaacat tagaaaataa atatattgat attgttgaag catttcaaat
     3001 tcaatgtgca agagaaatca ataatcaaga aattgaaaat attaaatcag agttgaataa
     3061 agttgaagca tattatgcac aaattgaaac tcaaattcaa caaacttcaa ctgaaaaaat
     3121 agcaacagaa aaaacatcgt atctaataaa ttatatgaac gctgtggcat agaaaggcgg
     3181 cgaaacatga cacacaaata tatatcaacg caaatgttga tcatttttac tgcattaatg
     3241 attattgcca atttttacta catatttttt gaaaaaattg gctttttact cgttctatta
     3301 ttgggatgtg tattagttta tgtaggatat ctttattttc ataaaatacg tggccttttg
     3361 gcgttttgga taggcgcgct attaattgca ttcacattat tgtctaataa gtatacaatc
     3421 atcatcttgt tcgtcttttt attactactt attgtgcgtt atttaataca caagtttaaa
     3481 cccaaaaaag tagttgcgac ggatgaggtt atgacttcac catcttttat taaacaaaag
     3541 tggtttggtg agcaacgtac accagtttat gtatataagt gggaagatgt ccaaattcaa
     3601 catggaattg gcgacctaca tattgactta acaaaagctg caaatattaa ggaaaataat
     3661 accattgttg ttagacacat tttaggtaaa gtgcaggtta tattgccggt taattacaat
     3721 attaatttac atgtagctgc tttttatgga agtacttacg tgaatgaaaa atcatataaa
     3781 gttgaaaata acaatattca tattgaagaa atgatgaaac cggataacta tacagttaat
     3841 atctacgtat caacgtttat cggagacgta gaggtgattt atcgatgaac cactacatta
     3901 gaacaattgg ttcaatgctc atcttagtat atagcatgct agctgcattt ctgttcatcg
     3961 ataaagtttt tgtaaatatc atctattttc aaggtatgtt ttatacacaa atattcggaa
     4021 taccagtctt tttattttta aatctcatca tcatattatt gtgtattatt gttggttcgg
     4081 tactcgctta caaaatcaat cagcaaaatg attggattaa gacgcaaatt gagcgttcaa
     4141 tggaaggcga aacagttggc attaatgatc aaaatataga aatatatagt gaaacgttag
     4201 acttatacca tacacttgta cctttaaatc aagaattgca taagttgcga cttaaaactc
     4261 aaaacttaac caatgaaaat tataatatta atgatgtgaa agttaaaaag attattgaag
     4321 atgaacgtca aagactagca cgagaacttc acgattctgt tagtcagcaa ctttttgcgg
     4381 caagtatgat gctttctgct atcaaagaaa cgaagttaga accaccatta gaccaacaaa
     4441 ttccgatttt agagaaaatg gttcaagatt cgcagctaga aatgcgtgct ttgctgttac
     4501 atttaagacc gcttggttta aaagacaaat ctttaggtga gggtattaaa gatttagtta
     4561 ttgatttaca aaaaaaagtg ccaatgaaag ttgtgcatga aatacaagat tttaaagtgc
     4621 ctaaaggtat tgaagatcat ttgttcagaa ttacacagga agcaatttcg aatacattgc
     4681 gtcattcaaa cggtacaaaa gtgacagtag aattgtttaa taaagacgat tatttattgt
     4741 tgagaattca agacaatggt aaaggtttta atgttgatga aaaattagaa caaagttatg
     4801 gacttaaaaa tatgcgtgaa agagctttgg aaattggtgc aacgttccat attgtatcat
     4861 tgccagattc aggtacacgt atcgaggtga aagcaccttt aaataaggag gattcgtatg
     4921 acgattaaag tattgtttgt ggatgatcat gaaatggtac gtataggaat ttcaagttat
     4981 ctatcaacgc aaagtgatat tgaagtagtt ggtgaaggcg cttctggtaa agaagcaatt
     5041 gccaaagccc atgagttgaa gccagattta atattaatgg atttacttat ggaagacatg
     5101 gatggtgtag aagcgacgac tcagattaaa aaagatttac cgcaaattaa agtattaatg
     5161 ttaactagtt ttattgaaga taaagaggta tatcgtgcat tagatgcagg tgtcgatagt
     5221 tacattttaa aaacaacaag tgcaaaagat atcgccgatg cagttcgtaa aacttctaga
     5281 ggagaatctg tttttgaacc ggaagtttta gtgaaaatgc gtaaccgtat gaaaaagcgc
     5341 gcagagttat atgaaatgct tacagaacga gaaatggaaa tattattatt gattgcgaaa
     5401 ggttactcaa atcaagaaat tgctagtgca tcgcatatta ctattaaaac ggttaagaca
     5461 catgtgagta acattttaag taagttagaa gtgcaagata gaacacaagc tgttatctat
     5521 gcattccaac ataatttaat tcaatagttc ttatcgaatt aagaaaagtt acttacgcca
     5581 atcacaatat aacatcaaat atacactaaa aaacagaggt cttcgcaatc attagcgaaa
     5641 acctctgttt tgttgttatt tagtcttttt taagcttatc aacgattttg tcttttatat
     5701 ttttatcatc ttctggatag gtatcactag gatgttcgtc aacatgatac gttcgttgat
     5761 gtgttgcatt agtatcttca tcatattcgt acctagtgtt ctcattataa tgatttttat
     5821 tattgtcatc atgttctaat gctgcttctt caggtgtttt acctttaatt acactacgct
     5881 gatgaatgat tgcattgatt tcagcaccga caattataat aaaacttgtg atatataacc
     5941 atagtaacaa aatgatgata cctgcgatac tgccatatgt tttagaatag ttaccaaaat
     6001 ttgaaatata ccaaccaaaa ccaaatgacc cagccaacca aataattgaa gtaaatactg
     6061 cacctggtaa tactgactta agtttcgttt taacgttagg tgcaaccgaa tataacacga
     6121 taaatacgat aaatataata atgattggta acacaattct aataagatta aaaatccatt
     6181 tcacttgttc gtcaaatcca agtggaccga ataggaaatg actaattaca gaaccaagcg
     6241 ttggtaatgc tagagcaact acaaacacaa cgcccataac tacagtgaag acaacactta
     6301 gtagttttaa tacgattcca ttacggctat cttctacatc gtaagcaaca ttgaaagaat
     6361 tcataattgc agtcattcca tttgaagctg accaaattgc taaaatcaaa ccgatagata
     6421 ataagccacc actggagttt tgagttatat caccaattac actcttaatt agagtagatg
     6481 tttcagcggg tgcattactt aacatattag taatttgact ctgcttaata ttgaaaaatg
     6541 gtaataatgt taataggaaa agcaacatag ggaacatagc aagtacgaaa tggtaagtca
     6601 tttgcgctgc tagtcctgag gcatcatctt ttcctatgcg atagatgaga tatgataaga
     6661 aattagagtc tttcttatat tttgcaggtt tatttaatct tgataagaag aaaacctgat
     6721 catctttttt aggtttctta gattggaatt gttgaggttc aatatatgta cgatcaacat
     6781 caattttggg attactttta tttttctttt ttgactcctc ttgcgcttcc ttaacagaat
     6841 taaggtattt tgaagaagag tggtcctttt tcgacatgtg cactccttaa cctttctcta
     6901 tgtaatgttt tataaagtaa ctattagtat ttatccaatt ataaaaaaac taaacaatga
     6961 tatgctttaa aaatgataat attatagaat ttaattgatt taaatctttc ataggctatt
     7021 ttgatttaaa attaaaacaa aggcaatact gaaacaatgc agtttggcca atcattgtat
     7081 tatcatttaa taatgataac tacgaaaggc gtacaccaaa ttgttaagta ttaccttata
     7141 aaaacaaaat catcttagcg ttgatttttt ctattaacaa atgtttcttt cgcatccttt
     7201 aatgaacgtt ctaattcagg attattacga cgaatttctt caacaacatc tttccaatat
     7261 aaaacttcgt ctttaatctt gctgacttta gaaggcttac gagtgcggtt accatttttt
     7321 gcatctttaa ctgattgaac taaagcttga cgtgtagatt tatcagctaa actaattgca
     7381 ccaccaatta cggcaccaat taaaatacca ggaacaaatt tattttccat aaaaaactac
     7441 ccctctttca aatttgcatc ttttacgatg tagtctatta aattatcaca agatgataat
     7501 accatgtcgt atacaccttc aaaattattc gtgtagtatg gatctggtac atcactctct
     7561 tccatattac taaattctaa cagtttgaac aattgtccct taagattagg attgatagat
     7621 ttaatattat caacgttact ttgatccata gccacaatgt aatcaaaatc atctgtcgct
     7681 tcgaataatt cactaatcat gccatcaaat ggaatattgt gtttgttaag aattttttgt
     7741 gtaccttcat gaggcggctc tcctaaattc cagctaccag tacctcttga atgtactttt
     7801 atatcttgaa tatttctgtc tttaagtctt tgtcgcatga ttgcttctgc cattggagaa
     7861 cgacatatat tgccaagaca gacaaatgct acatctacca ttttgattcc tccaaactat
     7921 gtagttatat ccccatttta tagcgacttt aaacaataag aaagcagatt atataaaatt
     7981 ctattaaagt ttattaaatt gtgatacttt gataacataa ctattattag aggtgaacat
     8041 tgtggctatg acaaatgaag agaaagtatt agctattaga gagaagttaa atattgttaa
     8101 tcaaggatta ttagatcctg aaaaatataa aaatgcaaat gaagaagaat taacagatat
     8161 atatgatttt gttcaatcaa gagaaagatt gtcgccaagt gaagtgacag ctattgctga
     8221 cgctttagga caattgcgac acgactagga gtgggaattt tgactaatta caaagaaaag
     8281 ttacaacaat acgctgaact attagttaag gttggtatga atgttcaacc aaaacagcca
     8341 gtatttatta gatcatcagt agaaacatta gaattaacgc atttaattgt agaagaagct
     8401 tatcgttgtg gtgcttcaga tgtacgtgtt gtatatagcg atccaacctt aaaacgctta
     8461 aaatttgaaa atgaatctgt ggaacatttt gctaatcatg agataaaatc atatgatgtt
     8521 gaagcgagaa tggattacgt taaacgtggc gctgctaatt tagcacttat tagtgaagat
     8581 ccagatttaa tggatggtat cgatagccaa aaattacaag catttcaaca acaaaacgca
     8641 cgcgcattta aaggatatat ggaaagtgta caaaaaaatc aatttccatg ggtcgtagct
     8701 gcttttccat ctaaagcttg ggcgaagaga gtatatcctg aactatctgt agaagaagca
     8761 tatattaagt ttattgacga ggtattcgat attgtgcgaa ttgatggtaa cgatccagtt
     8821 gaaaattggc gtcaacacat cgctaattta agtgtttacg cacaaaaatt acagcaaaaa
     8881 aattaccatg cgttgcatta tgtatctgaa ggcactgatt taactgtagg tttagcgaaa
     8941 aatcatatat gggaagatgc tacaagttat gttaacggta aggaacaagc ttttattgct
     9001 aatataccaa ctgaagaagt atttacagca ccagatcgaa atagagtaga tggatatgtt
     9061 actaataaat tgcctttaag ttataatggc acaattatcg atcaatttaa attgatgttt
     9121 aaagatggag aaattattga tttttcagct gaaaaaggtg aagcggtatt gaaagattta
     9181 atcaatacag atgaaggttc aagaagatta ggtgaagttg cgttagtacc tgatgattca
     9241 ccgatatcaa atcgtaatac cattttttac aatactttat ttgatgaaaa tgcagcatgt
     9301 catttagcga ttggatctgc ttacgccttt aatattcaag gtggaacgga aatgactgtt
     9361 gaggaaaaga ttgcaagcgg attaaatgat tcaaacgtac atgtcgactt catgattggt
     9421 agtagtgatt tgactattta tggcatattc gaagatggtt caaaagaact agtatttgaa
     9481 aatggaaatt gggcatcaac attttaataa atgttatttt gaggtgctga gtaggaaatg
     9541 aaacatgtat ttaaaggtca aatacgtgat taaagtatag attgggagat aaaataatga
     9601 caaatcagga cagaccaatg aaatctatgt cagaatcaaa atgttataaa aatagacaag
     9661 ttttccctca agatacgaat caccatcata caatgtttgg tggtacattg atggctaata
     9721 ttgatgaaat tgcagcaatc acagctatga aacatgctgg tgcgcaagta gttaccgcat
     9781 ctacagactc agtagatttc ttaaagccga ttaaaacagg ggacatatta caatacgtag
     9841 cgatggtttc atacgctggg acaagttcga tggaagtggt cgttcaaatt agaattgacg
     9901 atgtatttaa taacaagcat gatttagctg cattaagtta cttaacattt gttgcgttag
     9961 atgatgaagg gaagcctaaa catgtacctg gagtatatcc agaagatgat gttgaaaaat
    10021 ggttttatga tacagcacca caacgtgttg aacgaagaaa agcacgtcgc attgaaagta
    10081 aacaaacaat tgaatattta gctcaagtgc aacatattag agactaaaaa tataaagaca
    10141 gattgttaag gatgaattga actgttatgt tcgaatattc cacctacaat ctgtcttttt
    10201 gattatgcaa atttatgttt ttagttaaaa tttaaccctt ttattataga ctattagtcc
    10261 tattaaatga cctttattat ttaagtagag tgctgccagc aattaattgt tttttatttt
    10321 gtaatagcat taaaatatgc ggagactact tatcgataaa gtgcttctcc gcataattaa
    10381 gcgtttttaa aattgtataa tttgtggttg tttgtgcata tgacgctcat tatctttaaa
    10441 atccatattc ggatagtaca tatttttaac aagaacattt ggtcctaaac aactaaactc
    10501 ggaacaatga caatttaatg atttagcaag atcagaggat aaccatttat caaatacatc
    10561 tgttaattta tctttttgta tattcgaaat tgtacctgtt tcatctccga aatcagttac
    10621 gattacatta cctgtaaata cattgacatt taaacgacta cggccatccg ggtcatttct
    10681 agtcgttaca tttttagcag ttcttaaacg tgatagtaac ttttgatctt catcatcttt
    10741 taagcatgga aacacaggca aagtaccaaa taacatccaa atatcttcat ctctgaaatc
    10801 caatatatca tgaattgtct ttttcatttc cgctagagtt aacacattta attgacttgc
    10861 aaagtcagct ggatacatag ggtgaatctc gtgtctgcta catttcatat catgaacgac
    10921 ttcttgatgt atttttcgta aatgtggtag cgtactttga ttgagcattg tttccgcaga
    10981 tacaaacatt ccttgttctg ataatgtaca tgcattcgaa atcatttgtt catataattt
    11041 taacttagct tttaacggtg gttgcttctt cattgcgcca aagccaacat ttgcgaattc
    11101 atcagttgtt ccccagttat gtgagatatg cataacatcg atatattcag caatatctaa
    11161 ataacgatct tgaggcaatg ttaggtttga attcatttgt gtatatatac ctcgattatg
    11221 tgcatacttt aatagaggtt taacaacatt tctaatagac tttttagaaa acattggttc
    11281 gccacctgta attgacatcg ttcgcagatt tggaatttca tcaagtctac gataaattaa
    11341 gtccatatct aaaggctcgg ggtcgacagt ttgtaaagta taaccaactg cacagtggct
    11401 acaacgcata ttacaaagat ttgtagttgt aaattcgatg ttacttaaag ttaattggcc
    11461 atgttcttta acatcgttat atgcttccca tggatcattg tgaatgctaa caggttgctt
    11521 caatttgacg ctattcatcg tcttaaactt ccttctttct atttggttca tatcggtatg
    11581 ataccacttc gtattatcac tcgtaacaaa ttatctctta attttagtta attgaaaagg
    11641 ggatttttaa aagtcaaaat aatttgaatg tcataaatat agctaagatt atatcgcttt
    11701 aacgcaattt ctgttaggat aataatgaaa aagaaaacaa aataaaagga gttaactttt
    11761 tatgaatgaa cagcaaacaa tcgaacagat aaaagcgcgt ttaaataagt ttattgagga
    11821 tatcgatcat gtaaatcctg atgaagtacg tgttgaagat atagatgaat ggattggatt
    11881 gttagatcag cttgaagaaa aggttaaatt agtatctaag taaatgccac ataagatgtg
    11941 ttagagttag atatcgcgac agtcgcttaa tgtttttata tcttgtatgt ttaatcactg
    12001 atttacttgt ctatagtatt tatatcaatg atatatagaa aggtgatttt caatgactaa
    12061 aaaagtagca attattctag caaacgaatt tgaagatata gaatattcaa gtcctaaaga
    12121 ggcattagag aatgctggct ttaatactgt agtgattgga gatactgcaa atagtgaagt
    12181 ttttggtaaa cacggtgaaa aagttactgt cgatgtaggc attgcagaag ctaaaccaga
    12241 agattatgat gcattattaa ttcctggagg gttttcacca gatcatttac gtggagatac
    12301 agaaggtcga tatggcacat ttgctaaata ctttactaaa aatgatgtac caacatttgc
    12361 catttgtcat gggccacaaa tactaataga tacagacgat ttaaaaggtc gtacgttaac
    12421 agcagtatta aatgtacgca aagatttatc aaatgcaggc gcacatgtag ttgatgagtc
    12481 agtagttgta gacaacaata ttgtaacaag tcgagtacca gacgatttag atgattttaa
    12541 tcgagaaatt gttaaacaat tacaatagaa gtattgtggt tatcgattgt taaaatttat
    12601 atattttcgt ttgaaatgaa atatggattt tagttttaaa aaatgaataa caaataaaag
    12661 gtcgtctcta ttggcattta atagggatgg tcttttattt ttataaaata taacaatatt
    12721 tgacatgatt aatatttaaa atcgcaatga tttatctata atcttttcaa aaaacaaaaa
    12781 agtaagtatg atggcgtata cgattgtaag cccttggact gattttccca aaaagtgtag
    12841 cctatttgtg atagtactgg taaactcaag gtatatacta agtgagtttt aaaagaagga
    12901 gcaaacgcat gaaaagaagc gataggtact caaactcaaa tgaacatttt gagcatatga
    12961 aacacgaacc tcactataat acgtattatc aaccagttgg caaaccgccg aaaaagaaaa
    13021 aaagtaaacg aatactattg aaaatattat taaccattct aatcattatc gcattgttta
    13081 ttggtatcat gtatttttta tctacacgcg ataatgtgga tgaactaaga aaaattgaaa
    13141 ataaaagtag ttttgtgtca gctgataaca tgccagagta tgttaaaggt gcctttattt
    13201 caatggaaga tgaacgattc tacaatcatc atggatttga tttgaaaggt acaactagag
    13261 ctttattttc aacgattagc gacagagatg tgcaaggtgg tagtaccatt acacaacaag
    13321 ttgtcaaaaa ttatttttat gataatgatc gttcatttac tagaaaagta aaagaattat
    13381 ttgtagctca tcgagttgaa aaacaatata ataagaacga aattttaagc ttttatttaa
    13441 ataatattta ctttggggat aatcaatata cgcttgaggg cgcagcaaac cattactttg
    13501 gaacaaccgt gaataaaaat agtacaacaa tgtctcacat tacagtttta caaagcgcta
    13561 ttttagctag taaagtcaat gcacctagcg tatataatat caataatatg tctgagaatt
    13621 ttacgcaacg tgtaagcacg aacttagaaa aaatgaagca acaaaattat atcaatgaaa
    13681 cacaatatca acaggctatg tcacaattaa atcgttaaat acttgcattc atgtgtaaga
    13741 tacttggaac aataatgtag aaggtataat tgaaatgcca taatgataaa gttgaattgt
    13801 ttctgcttaa taaattttac agatttgctt gtaatcacac tttcatttta aaagatgata
    13861 gtgtgatttt ttgttagcat cgaaaaatat ttaaagttaa acatttgcta taggaagtgg
    13921 cttattgaat aataaaagag tattgtatta aaacttgaga aagtaacggg gtgatccagt
    13981 gccgaagatt actaaaatag aagttcaaaa gaaaaacaaa gaacgtttta atcttttttt
    14041 agatgaacaa tttgaaatgg gtatagatat cgatacatta gtcaaattta atttaaaaaa
    14101 agggcaacaa cttgaagctg ctgacatggc agagattcaa aagtatgatc attatcgcat
    14161 aggtttaaat aaagcaatcc aatatttatc atataaaaag agaactgaaa aagaagttat
    14221 acaacattta caaaaagaag agatatcaga gcaagcgatt tctgaagtga ttgaatattg
    14281 ttatcgcgaa aagttaatcg accatcaaga ttatgcggaa agtttaaaaa atacaatgat
    14341 tcgcacgaca gataaaggac ctaaaattta tcaacaaaaa ctttatcaac ttggtataga
    14401 accaaatatc attgaaatgt ttacagaact ttatagagaa caacaggaac ttgatgatat
    14461 tatccaaatt gctgaaaaaa tatctaaaac aaaaaagggg ccacaaaata aggttaaaga
    14521 aaaagtaatg caatctttaa ttcaaaaagg gtttgaaatg gaaaccattc atgctgtatt
    14581 gaatgaaatg gattttacac aagatgaagc ggttttagac gatttattac aacgagattt
    14641 agaaaaaatt tataataaaa atcgaaagaa gtacacgcaa caaaaattaa tttcaaaaac
    14701 tatcgaaggc cttatgagaa aaggatataa atatgataaa attaaagcta aattagaaga
    14761 aagtggtatt gccgatggaa cagaagaaat tgagtgagat gtctgagcca gaattaagac
    14821 acgaaataca actttataaa gagaaaatga gaaaagctga gatgaatggt attttaaatg
    14881 aatatgacgt ttatcaaagt aaagtaattg ttgccgaaag ttacttagta gacagaaaaa
    14941 agattgaaat tggtaaaatt tataaactga ctgatggcag taatcaatat tttaaagtag
    15001 aaagattaaa aggtattttt gcgtgggggt ttagatttaa tagtgatgaa cccgaagaag
    15061 gcttacctat agcgttatta caattataga aggatgaaaa tagatgggaa gttcaattgt
    15121 tttaaagtta ctaaaagtaa cacactacta taggaataaa cagaataaaa aatggtattt
    15181 accttttgga tatgatgctc aagatatcga tttaaacaat attagtttac atatttatca
    15241 aggtgaagca ttaggtataa ttggtgaacc tgaatcttcc aaagcgatgg taggtcaatt
    15301 gttggcaggt gcaattaaac ccgataaagg taaagtagtt tgtactgaag atttgttcta
    15361 cggatatatt gaagatcaat cgttaattca tcaaactgtt gaagcttata cagcgcagtt
    15421 agttcaacta tttccatatg aaattaataa tcataaagct gaacagatta ttcaatatgc
    15481 acatttaggc gattataaaa cgaagccggt taaccatatt tcgaaagcgg catacgctca
    15541 attactatta agtattgcac gctcatcaaa atcaaatatt attattttaa atcatgttat
    15601 tgactattta acaccacaat ttatggaacg tgcgattgaa ttaacaaatg attatattga
    15661 aaataattta acgattgtgt caattggtga tgatattgat aaaatttcac aagtgagtaa
    15721 ctatatagct tggttttcac atggtcaatt aagaatggaa gggtcactta aacaagttat
    15781 tccatctttt aaagaacatg aacgtgatcg attaagtcta aactcaaaag aagaaattga
    15841 aaactttgat ttagattgga aaaagaatcg tacaagaata ccggagatga cctataattt
    15901 caaacgcgtt gagcgctata atcatgcaaa accgcctaag tttttagtgc gtttttggac
    15961 tttagcctca ggtactattt taggcttagc attgatgatg ttgctcattt tcaataatat
    16021 aggaattatt tcgataacag attttacgaa tcgtgctacg atgcaaaatg aaaataaaga
    16081 tccatatggc gaaaagttag cttatggaat tgcttttaat ggcagtgtgg atctgcaagg
    16141 ggataaacaa gtcacaattc caaaatatag tgtagttaca attactggtg aaaatagtaa
    16201 aaattatcgt gttaccgctg ataataagac ttactatgtt agtaaagata aattagaata
    16261 ttttaacccg gcaggtttat atcaaacgca tagttttaaa aaattagcgc catttatgaa
    16321 atcaaattat agtaa
//
LOCUS       DAKSQE010000060        16251 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.060, whole genome shotgun sequence.
ACCESSION   DAKSQE010000060 DAKSQE010000000
VERSION     DAKSQE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16251
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.060"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..764
                     /locus_tag="O0V33_001628"
     CDS             <1..764
                     /locus_tag="O0V33_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HCX8976257.1"
                     /translation="SITDYNYKKPLHNDYQILDKSKIFGSNSGSFVMYSMKKDKYYIY
                     NEKESRKRYSPNSTYKIYLAMFGLDRHIINDENSRMSWNHKHYPFDAWNKEQDLNTAM
                     QNSVNWYFERISDQIPKNYTATQLKQLNYGNKNLGSYKSYWMEDSLKISNLEQVIVFK
                     NMMEQNNHFSKKAKNQLSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHD
                     KYYFATHLSDGKPSGKNAELISEKILKEMGVLNGQ"
     gene            754..1134
                     /gene="blaI"
                     /locus_tag="O0V33_001629"
     CDS             754..1134
                     /gene="blaI"
                     /locus_tag="O0V33_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCX8976258.1"
                     /translation="MANKQVEISMAEWDVMNIIWGKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            1398..1991
                     /locus_tag="O0V33_001630"
     CDS             1398..1991
                     /locus_tag="O0V33_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCX8976259.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNAYGCEKIFSDHISGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEEKEDEKQGEVSN"
     gene            1963..3405
                     /locus_tag="O0V33_001631"
     CDS             1963..3405
                     /locus_tag="O0V33_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCX8976260.1"
                     /translation="MKNKEKYLTNFSEAKRKEATQKYNIIKPFILGKQSLSSISKSKG
                     IALSTLYRWNKLYKEQGLTGLIHNTRVDKGEHKLKQNIIDEIKRLALKNKRNSIATIH
                     RKIANYCIENNFYKPSYKQVYSIIKAMPKSVIDFSHQGEKYYQNKYDLIQIRESSRPN
                     EIWQADHTLLDIYILDQKGNINRPWLTIIMDDYSRAIAGYFISFDAPNAQNTALTLHQ
                     AIWNKNNTNWPVCGIPEKFYTDHGSDFTSHHMEQVAIDLKINLMFSKVGVPRGRGKIE
                     RFFQTVNQTFLEQLPGYINNNDTSSDLIDFQNFEEKLRYFLIEDYNQKEHSAIQSTPI
                     NRWNSNHFFPNMPSSLEQLDLLLLEIPKSRKIHSDGIHFQGFRYSNTNLTAYVGEYVL
                     IRYNPNDMAEIRVFYRDEFLCTAISPDLADYSIDIKEIQHARSQRRKHLKQNIASPST
                     TDLIKEEKSYGYSPQETTKNVKKLKRYRND"
     gene            3398..4213
                     /locus_tag="O0V33_001632"
     CDS             3398..4213
                     /locus_tag="O0V33_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCX8976261.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSS
                     RYYTNWNTIEKQVNHRGWEDLASKTTDDILSVNKIFYTAPAEKQTRLSNDLYSISASI
                     DLGQKLHIVNKYGHDHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMI
                     FIGMPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWQDLGFDLKLEDFTDY
                     EAITTIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
     gene            4659..5501
                     /locus_tag="O0V33_001633"
     CDS             4659..5501
                     /locus_tag="O0V33_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCX8976262.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHITADLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVPGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDDGESIDAYILRETL
                     VDNN"
     gene            complement(5625..5936)
                     /locus_tag="O0V33_001634"
     CDS             complement(5625..5936)
                     /locus_tag="O0V33_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCX8976263.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQFINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            6023..7099
                     /locus_tag="O0V33_001635"
     CDS             6023..7099
                     /locus_tag="O0V33_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCX8976264.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGNQPLSGELGKGTLIRIKDGTMILKPAFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            7164..7475
                     /locus_tag="O0V33_001636"
     CDS             7164..7475
                     /locus_tag="O0V33_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCX8976265.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            7575..8432
                     /locus_tag="O0V33_001637"
     CDS             7575..8432
                     /locus_tag="O0V33_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HCX8976266.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            8461..9099
                     /locus_tag="O0V33_001638"
     CDS             8461..9099
                     /locus_tag="O0V33_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCX8976267.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEITNEGNVVGYNIFEISKDITIEETGHIKLTDELVNVFQKRISEAGFDYKLNAD
                     LSPKFVVGYVETKDKHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVM
                     PSGMVIKDAELRGVASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            9120..12944
                     /locus_tag="O0V33_001639"
     CDS             9120..12944
                     /locus_tag="O0V33_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCX8976268.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEQRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKSKTHNI
                     YSNNTNHRAKDSTTDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKSSEKVESDKQ
                     KYDKYVAKTQTSQNKHLEQEKQKDSVVKQGTASKSSDENVSSTTKSTPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSTEASQLHTNEIEDEAVSNDENKKASIQNEDTDDTHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSDVTSQRQSALQHNVEVNNQDELKNQSRLIADS
                     EEDGATNEEEYSGSQIDDAEFYELNDTEVDEDTTSNSEDNTNRDASEMHVDAPKTQEH
                     AVTESQVNNIDKTVDNEIELAPRHKKDDQTNLSVNSLKTNDVNDGHVVEDSSMNEIEK
                     QNAEITENVQNEAAESKQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVPELKPVQSKQAASESKTATQNTPSSSTDSQESNTNAYKTNNMTSNNVE
                     NNQLIGHAATENDYQNAQQYSEQKPSADSTQTEIFEESQDDNQLENEQVDQSTSSSVS
                     EVSDITEESEETTHQNNTSGQQDNDDQQKDLQLSFSNQNEDTANENRPRTNQPDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIEPDEDWITDKKKELNDALFYFNAPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            12968..14281
                     /locus_tag="O0V33_001640"
     CDS             12968..14281
                     /locus_tag="O0V33_001640"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCX8976269.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKESDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVILFMGAGDIQKLQNAYLDKLGMKNAF"
     gene            14355..14846
                     /locus_tag="O0V33_001641"
     CDS             14355..14846
                     /locus_tag="O0V33_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCX8976270.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            14920..16110
                     /locus_tag="O0V33_001642"
     CDS             14920..16110
                     /locus_tag="O0V33_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976271.1"
                     /translation="MTQQQNNKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAFTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEASANNLSDTSQEAQEIQEAKREAQTETDKSAAVSNEESKASALKAQQAAI
                     KEEASANNLSDTSQEAQAIQEAKKEAQAETDKSADVSNEESKASTLNVSKEESQAERL
                     ANAAKQKQAKLTPGSKESQLTEALFSEKPVAKNDLKEIPLLVTKKNDVSETVNTDNKD
                     TVKQKEAKFENGVITRKADEKTPNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTSGQ
                     KKQHNKKASQGAKKQSSSSNSTTKTNQKNSKATNAKSSNASKKSNAKVEKAKSKIEKR
                     TFND"
BASE COUNT         6335 a         2224 c         2950 g         4742 t
ORIGIN      
        1 aatccataac tgattataat tataaaaagc ccttacacaa cgattaccaa attttagata
       61 aaagtaaaat ttttggttca aatagtggat ctttcgtcat gtacagcatg aagaaagaca
      121 aatactatat ttataacgaa aaagaaagta gaaaaaggta ttctcctaat tcaacttata
      181 aaatttattt agctatgttt ggacttgacc gacatattat aaatgatgaa aattcacgta
      241 tgtcatggaa tcataagcat tacccttttg atgcttggaa taaggaacaa gatttaaata
      301 cagcaatgca aaattcagtt aattggtact tcgaacgtat tagcgatcaa ataccaaaga
      361 actatactgc gactcaactc aagcaattaa attatggtaa taaaaatttg ggaagttata
      421 aaagctattg gatggaagat agtttgaaaa tatctaatct tgaacaagta atagttttta
      481 aaaatatgat ggaacaaaat aaccatttta gtaaaaaagc aaagaatcaa ttatcttctt
      541 cattattgat taagaaaaat gaaaagtatg aactgtatgg gaaaacaggt acaggtatag
      601 taaacgggaa gtataataat gggtggtttg taggttacgt aattacaaat catgataagt
      661 attattttgc tacacattta tcagatggaa agccatctgg gaaaaatgct gaattaatca
      721 gtgaaaaaat attaaaagaa atgggtgttt taaatggcca ataagcaagt tgaaatatct
      781 atggctgaat gggatgttat gaatataata tggggtaaaa aatcagtatc agctaatgaa
      841 attgtagttg aaattcaaaa atataaagaa gttagcgata aaacgattag aacattaatc
      901 acaagactat ataaaaaaga gattataaaa cgatacaaat cagagaatat ttatttttac
      961 tcatcaaata ttaaagaaga cgatattaaa atgaaaactg ctaaaacctt tcttaataaa
     1021 ctgtatggag gggacatgaa aagtttagtg ctgaattttg cgaaaaatga agaattaaat
     1081 aacaaagaaa ttgaagaatt gcgagacatt ttaaatgata ttagtaaaaa gtaattagag
     1141 atatgtttca aacataatac atatgtaaac tatgcttgac tcttattaaa ttagttttgt
     1201 taataacatt taaaatatat tattaaagat gagagtgtac agaaaataac cattttctgt
     1261 acactctctt tttatgccga aataagtgag taatacatat attttcgtgt tcaaaaaccc
     1321 aacaaaaaat ctatgttcac aaaagacctt gaaattaagt tatggtacac tgtatatgta
     1381 tataggaggt ttgtattttg aaaataggtt atgcaagagt ttcaactgga ttacaaaatc
     1441 tgaatttaca ggaggaccga ttaaacgcat atggttgtga aaagatattt agcgaccata
     1501 ttagtggttc aaaaagtaaa agacctggat tagataaagc aattgaattt gcgagatcag
     1561 gagatacgat tgttgtttgg agactcgaca gactaggacg taatatggag gatttaatca
     1621 cattagttaa tgagcttaac gaacgaggtg taagtttcca tagtttagaa gaaaatataa
     1681 cgatggataa atcaagttct acaggacaat tattatttca tttattcgca gcgtttgcag
     1741 aatttgaacg aaatttaatt ttagaacgtt cttcagctgg ccgaattgca gcacgtgcta
     1801 gaggacgcta tggaggtaga cccgaaaaat taaatcaaaa agatttaaac ttacttaaga
     1861 cactttatga taatggcacg ccaattaaga caattgcaga gcagtggcaa gtttcacgta
     1921 caacgattta tcgttacctc aataaattgg aggaaaagga agatgaaaaa caaggagaag
     1981 tatctaacta atttttctga agccaaacgt aaagaagcta ctcaaaagta caatattata
     2041 aaacctttta tcttgggcaa gcaatcttta tcaagcattt ctaaaagcaa aggtattgcg
     2101 ttaagtacac tttataggtg gaataaatta tataaggaac aaggtcttac tggtctaatt
     2161 cataatacaa gagttgataa aggtgagcat aaattaaaac aaaacataat agacgagatt
     2221 aaacgccttg cactcaagaa taaaagaaat agtattgcta cgattcatag gaaaatcgct
     2281 aattattgta tagagaataa tttttataaa ccaagttata aacaagttta tagtattatt
     2341 aaagcaatgc ctaagtctgt tattgatttc tctcaccaag gtgaaaaata ctatcaaaat
     2401 aagtacgatt taatacaaat aagagaatcc tcaagaccca atgaaatatg gcaagccgac
     2461 catactttgc tagatattta tatattagat caaaaaggta atatcaatag accatggcta
     2521 accattatta tggatgacta tagtcgtgca attgcaggct actttataag ttttgatgct
     2581 cctaatgctc aaaacacagc attaacacta caccaagcaa tatggaataa gaataatacc
     2641 aattggccag tatgtggtat acccgaaaaa ttctacacag atcacgggag tgactttacc
     2701 tcgcaccaca tggaacaagt cgctattgat ttgaaaatta acttgatgtt ttcaaaagtt
     2761 ggtgtccctc gtggtcgcgg gaaaatagaa cggttttttc agacagttaa ccaaactttc
     2821 ttagaacaac ttcctggata tataaacaat aatgatactt ctagcgattt aatagatttt
     2881 caaaatttcg aagaaaaatt acgatacttt ttaattgaag attataatca aaaagagcac
     2941 agtgctatcc agagcactcc tatcaatcgg tggaacagta atcatttttt tcctaatatg
     3001 ccaagcagtt tagaacaact tgatttatta ttattagaaa tacctaaatc tagaaaaatt
     3061 cattcagatg gtattcattt tcaaggcttt agatacagta atacaaacct aactgcttat
     3121 gtaggtgagt atgtattaat tcgttacaat cccaatgata tggctgaaat acgcgttttt
     3181 tatagagatg aatttctttg tacagcaata tcccctgatt tagctgatta ttcaattgat
     3241 ataaaagaga tacaacacgc acgtagtcag agaagaaaac acttaaaaca aaacattgct
     3301 agtccaagca ccactgattt aattaaggaa gaaaaaagtt atggttactc acctcaagaa
     3361 acgaccaaaa acgtcaaaaa attaaagagg tatcgtaatg actaaaaatc agaatttcat
     3421 tgaaacgaaa gagtataaac gatttgctga attttgcgat gcttgtatca aatatcaata
     3481 tattgggatc tgctatggtc aacctggtgt cggaaaaact ttatcttcta ggtattacac
     3541 aaattggaat actattgaaa agcaagttaa tcatagaggg tgggaagatc tagctagtaa
     3601 aacgactgat gatatattat cggtgaataa gatattttat actgcaccgg ctgaaaaaca
     3661 aacaagatta agtaatgatc tatatagtat tagtgcaagt attgatttgg gtcaaaaatt
     3721 acacatcgta aataagtatg gtcatgatca tagtaaacat tatagtgata tgtttaaata
     3781 tattgattta attatagtag atgagattga tcgccttaaa gtgcaacatt tagagcaatt
     3841 aagagctatt tatgatgaac ataatttagc aatgatattt attggtatgc caggtataga
     3901 gaaaaaacta tctcgttatc ctcaactata ttcgcgtata ggttttgcac atgaatttga
     3961 taatctaagt aaagacgaga cgcatcatat attagaatat aagtggcaag atttaggatt
     4021 tgatctaaaa ctcgaagatt ttactgatta tgaggcaata acgacgatta ttaaaattac
     4081 aaaagggaat tttaggctga ttcatcgttt atttgcgcag atagatagaa ttatggatat
     4141 aaatggctta gataaaatca gtacagaagt tgtagaaaca gctagagata gtttagtcat
     4201 aggtattcgt taatagaaaa agcaatgtac tttaatatag gacattgctt ttctcattta
     4261 ctaggtaaaa tcattctcat atatcaagta aagtaacata tggtggaaag gtggaacttt
     4321 ttgtttttta atacaaaaaa gcgctattgc ctctataatt taaagtgacc aaacccaaac
     4381 taaaggagac aatgcgccta tgaacaatgt tatactaaat acctttgatt tgaaagaata
     4441 aagcttgcat tttacttgga aattttaatt attaatttaa aattacagtc cgatggttat
     4501 gatgcattta catataatat gattgcatta atttttataa gtagttggaa gtgtattagt
     4561 gtggggcgtt tcatttttgt tcgcatttat gaatagtgat atgatgtaat aaacacatgc
     4621 taattgtaat aacgaatata gaaagaatag gtgatgttat gaaaatcggg gatatatcta
     4681 ttcattatct aaatggtggc aatacaaaaa tggatggcgg tgcaatgttt ggtgttgttc
     4741 cgaagccgtt gtggtcaaag caatacaatg caaatgaacg aaatcaaatc aatttaccga
     4801 cacatccaat tttgattcaa acggctcaat ataatttgat tatagatgcg ggtattggta
     4861 atggtaaatt atctgaaaag caattacgta attttggtgt agatgaagaa agtcatataa
     4921 ctgctgattt ggcaaattat aatttaacgc caaaggatat tgattatgta ctaatgacac
     4981 atatgcattt tgatcatgct gccggtttga ctgatcaagc gggacatgca atttttgaaa
     5041 atgcgattca tgttgtgcaa caagatgagt ggcatgagtt tattgcacct aatataagaa
     5101 gtaaatcaac ttactgggat aagaataaag gcgattatag taacaagttg attttattcg
     5161 aaaaacattt tgaaccggtt ccgggtatca agatgcaaca tagtggaggt catagctttg
     5221 gccacacaat cattacgatt gaaagtcaag gagataaagc agttcatatg ggtgatatat
     5281 tcccgactac tgcacataaa aatcctctat gggtaacagc atatgatgat tatcctatgc
     5341 aatcaattcg tgaaaaggaa cgcatgatac catattttat ccagcaacaa tattggttct
     5401 tgttttatca tgatgaaaac tactttgctg taaaatacag cgatgatggc gaaagtatag
     5461 atgcatatat tttacgtgaa acattagttg ataataacta aaaaaagatg tattactaaa
     5521 caaattttca agaataaaaa attgagccac atcctatctt actaattagg gtgtggctca
     5581 tttttaagtt ttacgatcca aatcaaatat ggataaaatt cgtattaacg ctctacaatg
     5641 ttaatgactt caccagtata tgcatctgca taaaaatcat aatgaatatt ttgaccattt
     5701 ttaatagttg taattccacc ttgataaact aaacggtatt tatcagtttc aggatgaacg
     5761 aatggttcat aaacgatata tgagcctttg acattcataa aatatgattt tacttcatta
     5821 agtactttaa cgggattata gcgtttacga ttaataaatt ggatgattga aatggtagaa
     5881 ataattacaa tggctactgc aattattgtt ggaattatat atttcagttt agtcatcatt
     5941 tatgctccct tgatttcaaa ttcatatcat tagtttacca tattgaagat gatataataa
     6001 cttataagga gtgagaactt ttatgaacat aaataaaaaa gtaacattac aacgaattca
     6061 aactttaact gagcttcatg gggcaccagg ttttgaagaa gaagtaaaaa attatatgac
     6121 tcagcaaatg gcgccgtacg tagatgaatt tattgaaaat cgtatgggtg gtttttttgg
     6181 tgttaaaaaa tctaaaaatc caaatgcaaa acgtgtaatg attgcagcac atatggatga
     6241 aattggattc atgattacaa acatcactaa aaatggaatg attcaattca caaatttagg
     6301 tggtgttgca aatgatattt ggcaaggaca acgcttagta attaaaaata gaaatggcga
     6361 taaaattatc ggtgttgttt ctaatatacc taaacatttt cgtactggta gtgaaggtgc
     6421 accggaaatt aaagatttaa cattagatat aggtgctcaa aatgaagatg aagtgcgtga
     6481 gcgcggaata gatataggag atacaattgt acctcacacg ccattcacac agttatctga
     6541 acatcgatat agtgctaaag catgggataa tcgttatggt tgtgtcttgg caattgaaat
     6601 actagaatta ttaaaagata tagaattaga tgtagacttg tatgttggcg caaatgttca
     6661 agaagaggtt ggattacgag gtgcgaaagc atctgcagag atgatagacc cagacgttgc
     6721 atttgttgtc gattgttcac ctgccaatga cgttaaaggt aatcaaccat tatccggtga
     6781 acttggtaaa ggtacgttaa ttcgcataaa agacggtaca atgattttaa agcctgcatt
     6841 tagagactat ttattaaagt tagtagaagc acatgacatt gaacatcaat actatatgtc
     6901 acctggtgga acagatggtg gagaaattca taaagctaat attggtattc caactgcagt
     6961 tattggtgta tgtgcacgat atattcatag tacagactca gtatttgata taagagacta
     7021 ttttgctgct agatctttac tttcagaagc catttgtaat ttagataaca atcaaataga
     7081 aacattacaa tataaataat cgggtaataa caactattat ttctaaatag ttatatataa
     7141 tcattaatta aggagacata aaaatgaaac aacttgaatc agaacaacaa tttgaatctt
     7201 taaaacaagg tgctacagta tttgaattca ctgcaggctg gtgtccagat tgtagagtga
     7261 tagaaccaga tttaccggaa ttagaagcga gatatcctat gtttgacttc gtatcagtag
     7321 accgtgataa atttatggat atttgtattg aaaatggtat tatggggatt ccaagttttc
     7381 tagtatataa aaatggagaa ctgcttggaa gttatattgg aaaagaacga aaatcaattg
     7441 aacagataga tgcattttta gctcaatacg tgtaatttag actagagaaa aacggggtaa
     7501 tactcgtttt tctctgttac tatgtgttga tttattgtaa actattataa ggtgcgaaat
     7561 taggagtgtt acatatgaat acctttcaaa tgagagataa attaaaggaa cgtttaagcc
     7621 atttagacgt tgattttaaa tttaatcgtg aagaagaaac tttgcgtatt tatcgaacag
     7681 ataataacaa aggtatcacg attaaactta acgctatagt cgcaaaatat gaagataaaa
     7741 aagaaaaaat tgtagatgaa attgtttatt acgttgatga agctattgca caaatggcag
     7801 ataaaaccct tgagagtata tcatctagtc aaattatgcc tgtcattaga gcgactagct
     7861 ttgataaaaa aactaaacaa ggtgttcctt ttatctatga tgaacatact gcagaaacag
     7921 cagtttatta tgcagtcgat ttaggaaaat catatcgtct aattgatgaa agcatgttag
     7981 aggatttgaa gttaactgaa caacaaataa gagaaatgtc tctgtttaat gttagaaaat
     8041 tatcaaattc atatacgact gatgaagtaa aaggtaatat tttttacttt attaactcaa
     8101 atgacgggta tgatgcaagt aggattctaa atactgcatt tttaaatgaa attgaggcac
     8161 aatgtcaagg tgaaatgctc gtagcagtgc cacaccaaga tgtgttaatt attgcagata
     8221 tacgcaataa aacaggatat gatgtgatgg cacatttaac aatggaattt ttcactaaag
     8281 gtctagttcc aattacatca ttatcctttg gatataaaca gggtcatctt gaaccgatat
     8341 ttattttagg taaaaataat aaacaaaaaa gagatccaaa cgtgattcag cgtttagaag
     8401 caaatcgtcg taaatttaat aaagataaat agaaataatt ggataaggag ttttgtcata
     8461 atgaatttat tttacaatcc taaatatgta ggagatgtcg catttttaca aattgaacca
     8521 gttgaaggtg aattaaacta caataaaaaa ggtaacgttg ttgaaattac taatgaaggt
     8581 aatgttgtag gttataatat ttttgaaatt actaatgaag gtaatgttgt aggttataat
     8641 atttttgaaa tttcaaaaga tataacaatt gaagaaacag gtcatattaa attaactgat
     8701 gaacttgtaa atgtattcca aaagcgtatt tcagaagctg gttttgatta taaattaaat
     8761 gctgatctat caccgaaatt tgtagttggc tacgttgaaa ctaaagacaa acatcctgat
     8821 gcagataaat taagtgtact aaatgtaaat gttggaaatg acacattaca aattgtatgt
     8881 ggcgcgccta acgttgaagc tggacagaaa gttgttgttg ctaaagtagg tgcagtgatg
     8941 cctagcggta tggtaattaa agatgctgaa ttacgtggtg ttgcctcaag cggtatgatt
     9001 tgttcaatga aagaattgaa tttacctaat gcacctgaag aaaaaggtat tatggtatta
     9061 aatgacagct atgaaattgg acaagcattt tttgaataat taaggaaggt agtgaaaata
     9121 tgagctggtt tgataaatta ttcggcgaag ataatgattc aaatgatgac ttgattcata
     9181 gaaagaaaaa aagacgtcaa gaatcacaaa atatagataa cgatcatgac tcattactgc
     9241 ctcaaaataa tgatatttat agtcgtccga ggggaaaatt ccgttttcct atgagcgtag
     9301 cttatgaaaa tgaaaatgtt gaacaatctg cagatactat ttcagatgaa aaagaacaat
     9361 accatcgaga ctatcgcaaa caaagccacg attctcgttc acaaaaacga catcgccgta
     9421 gaagaaatca aacaactgaa gaacaaaatt atagtgaaca acgtgggaat tctaaaatat
     9481 cacagcaaag tataaaatat aaagatcatt cacattacca tacgaataag ccaggtacat
     9541 atgtttctgc aattaatggt attgagaagg aaacgcacaa gtcaaaaaca cacaatatat
     9601 attctaataa tacaaatcat cgtgctaaag attcaactac agattatcac aaagaaagtt
     9661 tcaagacttc agaggtaccg tcagctattt ttggcacaat gaaacctaaa aagttagaaa
     9721 atggtcgtat ccctgtaagt aaatcttcag aaaaagttga gtcagataaa caaaaatatg
     9781 ataaatatgt agctaagacg caaacgtctc aaaataaaca tttagaacaa gagaaacaaa
     9841 aagatagtgt tgtcaagcaa ggaactgcat ctaaatcatc tgatgaaaat gtatcatcaa
     9901 caacaaaatc aacacctaat tattcaaaag ttgataatac tatcaaaatt gaaaacattt
     9961 atgcttcaca aattgttgaa gaaattagac gtgaacgaga acgtaaagtg cttcaaaagc
    10021 gtcgatttaa aaaagcgttg caacaaaagc gtgaagaaca taaaaacgaa gagcaagatg
    10081 caatacaacg tgcaattgat gaaatgtatg ctaaacaagc ggaacgctat gttggtgata
    10141 gttcattaaa tgatgatagt gacttaacag ataatagtac agaggctagt cagcttcata
    10201 caaatgaaat agaggatgaa gctgtatcaa atgatgaaaa taaaaaagcg tcaatacaaa
    10261 atgaagacac tgatgacact catgtagatg aaagtccata caattatgag gaagttagtt
    10321 tgaatcaagt atcgacaaca aaacaattgt cagatgatga agttacggtt tcggatgtaa
    10381 cgtctcaacg tcaatcagca ctgcaacata acgttgaagt aaataatcaa gatgaactaa
    10441 aaaatcaatc cagattaatt gctgattcag aagaagatgg agcaacgaat gaagaagaat
    10501 attcaggaag tcaaatcgat gatgcagaat tttatgaatt aaatgataca gaagtagatg
    10561 aggatactac ttcaaatagc gaagataata ccaatagaga cgcgtctgaa atgcatgtag
    10621 acgctcctaa aacgcaagag cacgcagtaa ctgaatctca agttaataat atcgataaaa
    10681 cggttgataa tgaaattgaa ttagcgccac gtcataaaaa agatgaccaa acaaacttaa
    10741 gtgtcaactc attgaaaacg aatgatgtga atgatggtca tgttgtggaa gattcaagca
    10801 tgaatgaaat agaaaagcaa aacgcagaaa ttacagaaaa tgtgcaaaac gaagcagctg
    10861 aaagtaaaca aaatgtcgaa gagaaaacta ttgaaaacgt aaatccaaag aaacagactg
    10921 aaaaggtttc aactttaagt aaaagaccat ttaatgttgt catgacgcca tctgataaaa
    10981 agcgtatgat ggatcgtaaa aagcattcaa aagtcaatgt gcctgaatta aagcctgtac
    11041 aaagtaaaca agctgcgagt gaaagcaaga ctgcgactca aaacacacca tcatcaagta
    11101 ctgattcaca agagtcaaac acgaatgcat ataaaacaaa taatatgaca tcaaacaatg
    11161 ttgagaacaa tcaacttatt ggtcatgcag caacagaaaa tgattatcaa aatgcacaac
    11221 aatattcaga gcagaaacct tctgctgatt caactcaaac ggaaatattt gaagaaagcc
    11281 aagatgataa tcaattggaa aatgagcaag ttgatcaatc aacttcgtct tcagtttcag
    11341 aagtaagcga cataactgaa gaaagcgaag aaacaacaca tcaaaacaat actagtggac
    11401 aacaagataa tgatgatcaa caaaaagatt tacagctttc attttcaaat caaaatgaag
    11461 atacagctaa tgaaaataga cctcggacga atcaaccaga tgttgcaaca aatcaagctg
    11521 tacaaacttc taagccgatg attcgtaaag gcccaaatat taaattgcca agtgtttcat
    11581 tactagaaga accacaagtt attgagccgg acgaggactg gattacagat aaaaagaaag
    11641 aacttaatga cgcattattt tactttaatg cacctgcaga agtacaagat gtaactgaag
    11701 gtccaagtgt tacaagattt gaattatcag ttgaaaaagg tgttaaagtt tcaagaatta
    11761 cggcattaca agatgacatt aaaatggcat tggcagcgaa agatattcgt atagaagcgc
    11821 caattccagg aactagtcgt gttggtattg aagttccgaa ccaaaatcca acgacggtca
    11881 acttacgttc tattattgaa tctccaagtt ttaaaaatgc tgaatctaaa ttaacagttg
    11941 cgatggggta tagaattaat aatgaaccat tacttatgga tattgctaaa acgccacacg
    12001 cactaattgc aggtgcaact ggatcaggga aatcagtttg tatcaatagt attttgatgt
    12061 ctttactata taaaaatcat cctgaggaat taagattatt acttattgat ccaaaaatgg
    12121 ttgaattagc tccttataat ggtttgccac atttagttgc accggtaatt acagatgtca
    12181 aagcagctac acagagttta aaatgggccg tagaagaaat ggaaagacgt tataagttat
    12241 ttgcacatta ccatgtacgt aatataacag catttaacaa aaaagcacca tatgatgaaa
    12301 gaatgccaaa aattgtcata gtaattgatg agttggctga tttaatgatg atggctccgc
    12361 aagaagttga gcagtctatt gctagaattg ctcaaaaagc gagagcatgt ggtattcata
    12421 tgttggtagc tacgcaaaga ccatctgtca atgtaattac aggtttaatt aaagccaaca
    12481 taccaacaag aattgcattt atggtatcat caagtgtaga ttcaagaacg atattagaca
    12541 gtggtggagc agaacgcttg ttaggatatg gcgatatgtt atatcttggt agcggtatga
    12601 ataaaccgat tagagttcaa ggtacatttg tttctgatga cgaaattgat gatgttgttg
    12661 attttatcaa acaacaaaga gaaccggact acctatttga agaaaaagaa ttgttgaaaa
    12721 aaacacaaac acaatcacaa gatgaattat ttgatgatgt ttgtgcattt atggttaatg
    12781 aaggacatat ttcaacatca ttaatccaaa gacatttcca aattggctat aatagagcag
    12841 caagaattat cgatcaatta gagcaactcg gttatgtttc gagtgctaat ggttcaaaac
    12901 caagggatgt ttatgttacg gaagcagatt taaataaaga ataattatga gtaaggagtt
    12961 ttatataatg acacactatc attttgtcgg aattaaaggt tctggcatga gttcattagc
    13021 acaaatcatg catgatttag gacatgaagt tcaaggatcg gatattgaga actacgtatt
    13081 tacagaagtt gctcttagaa ataaggggat aaaaatatta ccatttgatg ctaataacat
    13141 aaaagaagat atggtagtta tacaaggtaa tgcattcgcg agtagccatg aagaaatagt
    13201 acgtgcacat caattgaaat tagatgttgt aagttataat gattttttag gacagattat
    13261 tgatcaatat acttcagtag ctgtaactgg tgcacatggt aaaacttcta caacaggttt
    13321 attatcacat gttatgaatg gtgataaaaa gacttcattt ttaattggtg atggcacagg
    13381 tatgggattg cctgaaagtg attatttcgc ttttgaggca tgtgaatata gacgtcactt
    13441 tttaagttat aaacctgatt acgcaattat gacaaatatt gatttcgatc atcctgatta
    13501 ttttaaagat attaatgatg tttttgatgc attccaagaa atggcacata atgttaaaaa
    13561 aggtattatt gcttggggtg atgatgaaca tctacgtaaa attgaagcag atgttccaat
    13621 ttattactat ggatttaaag agtcggatga catttatgct caaaatattc aaattacgga
    13681 taaaggtact gcttttgatg tgtatgtgga tggtgagttt tatgatcact tcctatctcc
    13741 acaatatggt gaccatacag ttttaaatgc attagctgta attgcgatta gttatttaga
    13801 gaagctagat gttacaaata ttaaagaagc attagaaacg tttggtggtg ttaaacgtcg
    13861 tttcaatgaa actacaattg caaatcaagt tattgtagat gattatgcac accatccaag
    13921 agagattagt gctacaattg aaacagcacg aaagaaatat ccacataaag aagttgttgc
    13981 agtatttcaa ccacacactt tctctagaac acaagcattt ttaaatgaat ttgcagaaag
    14041 tttaagtaaa gcagatcgtg tattcttatg tgaaattttt ggatcaatta gagaaaatac
    14101 tggcgcatta acgatacaag atttaattga taaaattgaa ggtgcatcgt taattaatga
    14161 agattctatt aatgtattag aacaatttga taatgctgtt attttattta tgggtgcagg
    14221 tgatattcaa aaattacaaa atgcatattt agataaatta ggcatgaaaa atgcgtttta
    14281 atatgtttat aatagagtag tatgggtatt tattattaat gacattatta catgttaatt
    14341 aggaggcgtt tttaatggat tggattttac caattgctgg aattatcgct gcgattgcat
    14401 tcttaatttt atgtatcggt atcgtagctg tattaaattc tgttaagaaa aacttagatt
    14461 atgttgcaaa aacacttgac ggtgtagaag gtcaagttca aggtattact cgtgaaacaa
    14521 cagatttact tcataaagta aaccgtttaa ctgaggatat ccaaggtaaa gtagatcgtt
    14581 taaactcagt agtagatgct gttaaaggta tcggtgactc agtacaaacg ttaaacagct
    14641 ctgtagatcg tgtaacaaat tcaattacac ataatatttc tcaaaatgaa gataaaatct
    14701 cacaagttgt tcaatggtca aatgttgcaa tggaaattgc agacaaatgg caaaatagac
    14761 actaccgtcg tggaagtgca aattacaaag ctaataatgt agcaactgat gcaaatcata
    14821 gctatacttc tcgagtagat aaataattaa taattaaaca aatgcactta aatgaggttg
    14881 ttacttattt aagtgcattt gttataggag gatatacaaa tgacgcaaca acaaaataat
    14941 aaaagaacat taaaaaataa acacacttat caaaatgaac cattaccaaa ccgtaaagat
    15001 tttgttgtta gttttataac tggcgcgctt gttggttcag ctttaggctt atattttaaa
    15061 aataaagttt atcaaaaagc agatgattta aaagtcaaag aacaagaact gtcgcaaaag
    15121 tttgaagaaa gaaaaacgca acttgaagaa acggttgcct ttacaaaaga acgtgttgaa
    15181 ggatttttaa acaaatctaa aaatgaacaa gcggcattga aggcacaaca agcagcaata
    15241 aaagaagaag caagtgcaaa taatttaagc gatacatcac aagaggcaca agagattcaa
    15301 gaagctaaaa gagaagcaca aacagaaacg gataaaagtg cggctgtatc aaatgaagag
    15361 tcaaaggcat cggcattgaa ggcacaacaa gcagcgataa aagaagaagc aagtgcaaat
    15421 aatttaagtg atacatcaca agaagcacaa gcgattcaag aagcgaagaa agaagcgcaa
    15481 gcagaaacag ataaaagtgc agatgtatca aatgaagaat caaaagcatc gacattaaac
    15541 gtatcgaaag aagagtcaca agctgaaaga ttagcaaacg ctgcaaaaca gaagcaagct
    15601 aaattaacac caggctcaaa agagagtcaa ttaactgaag cgttattttc agaaaaacca
    15661 gttgctaaaa atgacttgaa agaaattcct ctattagtta ctaaaaagaa tgatgtatca
    15721 gaaacagtta atacagataa taaagacact gttaaacaaa aagaagctaa atttgaaaat
    15781 ggtgttatta cacgtaaagc tgatgaaaaa acacctaata atacagctgt tgacaagaaa
    15841 tcaggtaaac aatctaaaaa aacaacacct tcaaataaac gaaatgcatc aaaagcatcg
    15901 acaaataaaa cttcaggtca gaaaaagcaa cataataaga aagcatcaca aggtgcaaag
    15961 aaacaaagta gttcaagtaa ctcaacgaca aagactaatc aaaaaaattc aaaagcaaca
    16021 aatgctaaat catccaatgc atcaaaaaaa tcaaatgcta aagttgaaaa agctaaaagt
    16081 aaaatagaga aacgtacatt taatgactaa gcatactaaa atcgtttaat aatcaatcat
    16141 agcagtcatc tatgtattat atttttaaaa attgaatttc aaaataaaag gtaggcattc
    16201 aggtgcttac cttttttgat atcatcagtt tattttgaaa ggcaacgcga t
//
LOCUS       DAKSQE010000061        15817 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.061, whole genome shotgun sequence.
ACCESSION   DAKSQE010000061 DAKSQE010000000
VERSION     DAKSQE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15817
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.061"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(149..1042)
                     /locus_tag="O0V33_001643"
     CDS             complement(149..1042)
                     /locus_tag="O0V33_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCX8976272.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKKAHPNAQF
                     KVNKDTGAYTYTYDKSNAPNNNHQNQSRTNDNHQHANQRDLNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLVLGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWEGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
     gene            1290..1583
                     /gene="esxA"
                     /locus_tag="O0V33_001644"
     CDS             1290..1583
                     /gene="esxA"
                     /locus_tag="O0V33_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HCX8976273.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            1666..4695
                     /gene="esaA"
                     /locus_tag="O0V33_001645"
     CDS             1666..4695
                     /gene="esaA"
                     /locus_tag="O0V33_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HCX8976274.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSQSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDSD
                     TTFIYNMSKQDFIAAGLNEDEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKATSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKTIADSVSGQLNQL
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTDVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNNLWNNAITSGVIGATGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLALVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            4695..5153
                     /gene="essA"
                     /locus_tag="O0V33_001646"
     CDS             4695..5153
                     /gene="essA"
                     /locus_tag="O0V33_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HCX8976275.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKAVNDAIVKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            5125..5367
                     /gene="esaB"
                     /locus_tag="O0V33_001647"
     CDS             5125..5367
                     /gene="esaB"
                     /locus_tag="O0V33_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HCX8976276.1"
                     /translation="MNQHVKVTFDFTNYNYGAYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            5380..6714
                     /gene="essB"
                     /locus_tag="O0V33_001648"
     CDS             5380..6714
                     /gene="essB"
                     /locus_tag="O0V33_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HCX8976277.1"
                     /translation="MVKNHDPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKEDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            6736..11184
                     /gene="essC"
                     /locus_tag="O0V33_001649"
     CDS             6736..11184
                     /gene="essC"
                     /locus_tag="O0V33_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HCX8976278.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQIYTEADYASFAYPSIQDTMTIGSNAYD
                     DMVIQSLMNAIIIKDFQSIQETQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSTVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKAYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSTFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSRSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGVATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPVAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRKIHDIISERK
                     RLLSQERVVNIEQYNKETGNSIPNVFLIIDNYDTVKESPFMEEYEEMMSKVTREGLAL
                     GVYIILSGSRSSAIKSAIFTNIKTRVALYLFENNELTNIIGSYKKGVKDVKGRAAIND
                     DNFTQFQIAQPFELAEGQTYNERIKNEVAQMKEFYVGDYPKHIPMMPDKVFMEDIREA
                     YDLEKIIHEEHKLPLGLDFEDVELVSLDLTSSSIVTAIKPTEMEKMNDVIMSSLSVYS
                     KNQFVILVDAEDNMSQYSEDVTSYYSAPSDLSNIRLGFKQEIEARKNGEKSIEECKIV
                     FINNIKRFNQLTGMTEDEIRVLFNEGQKVNIIIIASGLYSDTIGAFDRESKMMVRTIN
                     QALISHKISEQEFIRVKDRFGEPELKVGEMYYINNQEYQKIKLMEG"
     gene            11187..11453
                     /locus_tag="O0V33_001650"
     CDS             11187..11453
                     /locus_tag="O0V33_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwqI/YxiC family protein"
                     /protein_id="HCX8976279.1"
                     /translation="MIKLNQASVSKEISSIRTNGQGLKQSNGNVNLSKTNLVTFKEYV
                     NMFEDYQSALSNYENIIEQDTTAMDTTVTEIVENDREIAGQINK"
     gene            11476..11871
                     /locus_tag="O0V33_001651"
     CDS             11476..11871
                     /locus_tag="O0V33_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5082 family protein"
                     /protein_id="HCX8976280.1"
                     /translation="MSNKGEIRRQIANKEREKASKEAQLTDLKEDLRRLKDASKKLDT
                     AGEDFNKGQSSYNKVEISTSDWKGERRTKSDSKKKDVDSELKKVEQDFDDAKKAIKKD
                     IQDKEEEIKGVEGEISTINAAIDALKSKL"
     gene            11893..13563
                     /locus_tag="O0V33_001652"
     CDS             11893..13563
                     /locus_tag="O0V33_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000540847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCX8976281.1"
                     /translation="MGYKVDMSEVHNMQKSIDSSLSAINTKVSTLSSSINNLINTEGF
                     EGQTASSVKNYSKTFHLQSIKKIETINKDFKSDIAKSIRKFQSEVDNNASAILDEDEI
                     KKYKKDIDDALKDVFKSSKDANGAISDVSDLTTAKKIKTENLANKMGDFNKDIDQTVE
                     RLTTFDANNSIDGDKTDNLITELSGVNSYVKNMKPNRARISSTSGKIEGAIARHKTSE
                     ELVRWQTYMETLSENLYKTPGLSKAASDIITQAGREYYAIKAVGNGSALRGYKEYLKT
                     RDINKLINKMNKNQLKRMSLVLNTDRGNIKIKNAVKMASEFARNNPFKKGNLVNWMSK
                     VQGYQSVSAQILKKGLQDKNFKYTFGDAKKFFDTADMKKAAVTEFKNTFVPQNIRETF
                     FKKENLTSKTALKKNMSKFWNEDIKGGIKEFKLDFANKNIIGKLGKLLKLGGKALKPL
                     GFITAITDNIGKKSWQEKLVGMGVDLSAIGASAAAGAAVGTAIPLPVVGTVAGALAGA
                     AVGIALDYKIPYLDKSATELAKDGINSGINKVKSGFGKVTSGFKTVFG"
     gene            13579..14223
                     /locus_tag="O0V33_001653"
     CDS             13579..14223
                     /locus_tag="O0V33_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCX8976282.1"
                     /translation="MKIQEIVGNIKRAYLTPLIIFVILFSLFFDLIMYFYGNLQGKLP
                     IYLDVFLIFAAVVFILMYFQEKSGEVKVEKSNVIRYLTLNVVAGYSMVLLVASIYVFG
                     VAGYGFDVFNYWLGIILMLFVSWFALFLFYKNEFDSENPNKTVNVIAIIIKLSAFGGL
                     FYIRTVVPNTADEEKFITLSILINIAVDLLLVRSYFNYALYKSVKKDIENGVDD"
     gene            14223..14678
                     /locus_tag="O0V33_001654"
     CDS             14223..14678
                     /locus_tag="O0V33_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5085 family protein"
                     /protein_id="HCX8976283.1"
                     /translation="MEVGMLIMPFCVKITFEIDKNDNWLNALDTVNEFFMDKDIYPTG
                     PIIFQRELAGLGEYKYTAYIALNDELQDIPELNIKYIDCLEVGPTLSTKCFEEEEFER
                     AYKEIEYVANENNIKILNQPYYHVMVDYFGGTAFEIYAQVDLDESEVYG"
     gene            14671..15129
                     /locus_tag="O0V33_001655"
     CDS             14671..15129
                     /locus_tag="O0V33_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5085 family protein"
                     /protein_id="HCX8976284.1"
                     /translation="MDKNGYSYIMMKNVAYKTYFDVTMDDYEDILKDFTNLAISKGKT
                     ITGPLTFAVTQIDLQKRMNIDLFISVGKCFKSNEELSYRTYFCLDNMLHGRITSNNFI
                     TDEIALLEDMNEFASDNNLTFTSPYYHTMRKSFSGEQGWIDVKAKVYEND"
     gene            15153..15740
                     /locus_tag="O0V33_001656"
     CDS             15153..15740
                     /locus_tag="O0V33_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HCX8976285.1"
                     /translation="MIIFILLTVFALFYIAMIASLFKSEGFSIIGLILDIVILTTLIF
                     YYFVGASFVDNDLSNFLAFMNFGSFVYMYYAIKSLWVKPKLVNYIIAKEIGESKDVIE
                     EQELDLQTSKIRGIYFFIIAIALLIITKLRMQPELQADAISMNPVFIFIGVIIILIWL
                     VLDIYRKKKYGIFLFKTIVPLVVTTWIIIATIVLS"
BASE COUNT         6109 a         2089 c         2900 g         4719 t
ORIGIN      
        1 gttaacttta aggtgacaag ttattgcaat tttgaaggta gacatcatat tttataatcg
       61 aataacatac aaaataaagc gaccaatttt gcagtgttac gaattgtaaa ctgcgaactg
      121 gtcgcttttt attgatgtca tgattaattt aatggatgta attatatgat gaaacttctg
      181 aagcagagat ggttcttgat gaaacgatat attcaccaat ccagttcatt tctgaaatta
      241 gaatacttcc atcaatatta actttctcaa cgtaggctac atggccaaat ggaccgttta
      301 ctgtttgtaa aattgatcct cgtgttgggt gtctatctac tttgaagcca ttgcttgaag
      361 cttggccttc ccagttttta gcatctcccc aaaatgtact aatcgtgtgt ccatctttgg
      421 cacgtttatc aaagacatac catgtacatt gtccagcagt atataagttg ttcttacttg
      481 tgataagagg ctgatcaatg attttaccgt tacctaaaac taaaggttta ccgtcagcag
      541 tctttgcttt gtcattaaat tcggcgattt gtaattcgtc atacaattcg tctaaatcga
      601 tgcctgtaat aagccctttg ttatcttttg aaaaagcgtt atttaattca tcgtcattgt
      661 cttcaacatt cggtattgct ggtgtcaaag gattgcttgg tgacgtttga ggcggtgtgt
      721 gtgaatcaat tgcgtcatta atgtgcgtat actgaccact taatgaagaa tggtactgat
      781 tgttgttaag atcacgttga tttgcgtgtt gatgattgtc gtttgtacgt gactgatttt
      841 gatgattgtt gtttggcgcg ttgcttttgt cataagtata agtatacgcg ccggtgtctt
      901 tattcacttt gaactgtgcg tttgggtgtg ctttctttgc ttcttctaat gttttgctat
      961 cattcgtgta tgcttgagcc gagttaggcg acatactaaa taaagtaaga gttgtcatcg
     1021 tcagtaaaat tgttttcttc ataataacca tttaatcctt tatgtattta atttaatttt
     1081 agtatacaca tttatattac aaaaacgaat ggttaattaa aaatatatgg gtatattcaa
     1141 tatatttatt taaaaaaagc taaaaatact taaaaaacta tatacatata ctaataattt
     1201 atatattatt tgagtaagta tcactttttc aaaaaatagt gtccctaaaa agttttgata
     1261 aacttaaaat attcaggagg tttctagtta tggcaatgat taagatgagt ccagaggaaa
     1321 tcagagcaaa atcgcaatct tacgggcaag gttcagacca aatccgtcaa attttatctg
     1381 atttaacacg tgcacaaggt gaaattgcag cgaactggga aggtcaagct ttcagccgtt
     1441 tcgaagagca attccaacaa cttagtccta aagtagaaaa atttgcacaa ttattagaag
     1501 aaattaaaca acaattgaat agcactgctg atgccgttca agagcaagac caacaacttt
     1561 ctaataattt cggtttgcaa taagcattct gaaattggca aagtcacatt ttctaatgtg
     1621 gctttgctta tcatttttta agaaaacaac tgaaaggaaa taagcatgaa aaagaaaaat
     1681 tggatttatg cattaattgt cactttaatt attataattg ccatagttag tatgatattt
     1741 tttgttcaaa caaaatatgg agatcaatca gaaaaaggat cccaaagtgt aagtaataaa
     1801 aataataaaa tacatatcgc aattgttaac gaggatcaac caacgacata taacggtaaa
     1861 aaggttgagc tgggtcaagc atttattaaa aggttagcaa atgagaaaaa ctataaattt
     1921 gaaacagtaa caagaaacgt tgctgagtct ggtttgaaaa atggcggata ccaagtcatg
     1981 attgttatcc cagaaaactt ttcaaaattg gcaatgcaat tagacgctaa aacaccatcg
     2041 aaaatatcac tacagtataa aacagctgta ggacaaaaag aagaagtagc taaaaacaca
     2101 gaaaaagttg taagtaatgt acttaacgac tttaacaaaa acttggtcga aatttattta
     2161 acaagcatca ttgataattt acataatgca caaaaaaatg ttggcgctat tatgacgcgt
     2221 gaacatggtg tgaatagtaa attctcgaat tacttattaa atccaattaa cgacttcccg
     2281 gaattattta cagatacgct tgtaaattcg atttctgcaa acaaagatat tacaaaatgg
     2341 ttccaaacat acaataaatc attactgagt gcgaattcag atacattcag agtgaacaca
     2401 gattataatg tttcgacttt aattgaaaaa caaaattcat tatttgacga acacaataca
     2461 gcgatggata aaatgttaca agattataaa tcgcaaaaag atagcgtgga acttgataac
     2521 tatatcaatg cattaaaaca gatggacagc caaattgatc aacaatcaag tatgcaagat
     2581 acaggtaaag aagaatataa acaaactgtt aaagaaaact tagataaatt aagagaaatc
     2641 attcaatcac aagagtcacc attttcaaaa ggtatgattg aagactatcg taagcaatta
     2701 acagaatcac tccaagatga gcttgcaaac aacaaagact tacaagatgc gctaaatagc
     2761 attaaaatga acaatgctca attcgctgaa aacttagaga aacaacttca tgatgatatt
     2821 gtcaaagaac ctgattcaga tacaacattt atctataaca tgtctaaaca agactttata
     2881 gctgcaggtt taaatgagga tgaagctaat aaatacgaag caattgtcaa agaagcaaaa
     2941 cgttataaaa acgaatataa tttgaaaaaa ccgttagcag aacacattaa tttaacagat
     3001 tacgataacc aagttgcgca agacacaagt agtttgatta atgatggtgt gaaagtgcaa
     3061 cgtactgaaa cgattaaaag taatgatatt aatcaattaa ctgttgcaac agatcctcat
     3121 tttaattttg aaggcgacat taaaattaat ggtaaaaaat atgacattaa ggatcaaagt
     3181 gttcaactcg atacatctaa caaggaatat aaagttgaag tcaatggcgt tgctaaattg
     3241 aaaaaggatg ctgagaaaga tttcttaaaa gataaaacaa tgcatttaca attgttattt
     3301 ggacaagcaa atcgtcaaga tgaaccaaat gataagaaag caacgagtgt tgtggatgta
     3361 acattgaatc ataaccttga tggtcgctta tcgaaagatg cattaagcca gcaattgagt
     3421 gcattatcta ggtttgatgc gcattataaa atgtacacag atacaaaagg cagagaagat
     3481 aaaccattcg acaacaaacg tttaattgat atgatggttg accaagttat caatgacatg
     3541 gaaagtttca aagacgataa agtagctgtg ttacatcaaa ttgattcaat ggaagaaaac
     3601 tcagacaaac tgattgatga cattttaaat aacaaaaaga atacaacaaa aaataaagaa
     3661 gatatttcta agctgattga tcagttagaa aacgttaaaa agacttttgc tgaagagcca
     3721 caagaaccaa aaattgataa aggcaaaaat gatgaattta atacgatgtc ttcaaattta
     3781 gataaagaaa ttagtagaat ttctgagaaa agtacgcaat tgctatcaga tacacaagaa
     3841 tcaaaaacaa ttgcagattc agttagtgga caattaaatc aattagataa taatgtgaat
     3901 aaactacatg cgacaggtcg agcattaggc gtaagagcga atgatttgaa ccgtcaaatg
     3961 gctaaaaacg ataaagataa tgagttattc gctaaagagt ttaaaaaagt attacaaaat
     4021 tctaaagatg gcgacagaca aaaccaagca ttaaaagcat ttatgagtaa tccggttcaa
     4081 aagaaaaact tagaaaatgt tttagctaat aatggtaata cagacgtgat ttcaccgaca
     4141 ttattcgtat tattgatgta tttactatca atgattacag catatatttt ctatagctat
     4201 gaacgtgcta aaggacaaat gaatttcatc aaagatgatt atagtagtaa aaacaatctt
     4261 tggaataatg cgattacgtc tggtgttatt ggtgcaactg gtttagtaga aggattaatt
     4321 gtcggtttaa ttgcaatgaa taagttccat gtattagctg gctatagagc gaaattcatc
     4381 ttaatggtga ttttaactat gatggtcttc gtacttatta atacgtattt actaagacag
     4441 gtaaaatcta tcggtatgtt cttaatgatt gctgcattgg gtctatactt tgtagctatg
     4501 aataatttga aagcggctgg acaaggtgtg actaataaaa tttcaccatt atcttatatc
     4561 gataacatgt tcttcaatta tttaaatgca gagcatccta taggcttggc gctagtaata
     4621 ttaacagtac ttgtgattat tggctttgta ctgaacatgt ttataaaaca ctttaagaaa
     4681 gagagattaa tctaatgttg atgaatagcg tgattgcttt aactttttta acagcatcta
     4741 gcaataatgg cggacttaat attgacgtgc aacaagaaga ggaaaagcga atcaataatg
     4801 atttaaatca atatgataca acgctattta ataaagacag caaagcggtt aatgatgcga
     4861 ttgttaagca gaaaaaagaa cgacaacaac aaataaaaaa tgatatgttt caaaatcaag
     4921 cgagtcactc gactcgcttg aatgaaacta aaaaagtgtt attttccaaa tctaacttag
     4981 aaaagacttc ggagagtgat aaaagcccct atattcaaaa caagcaggag aaaaaaatat
     5041 tcccgtacat tttgatgtct gtaggggctt ttttgacttt aggatttgtc attttttcaa
     5101 ttcataaagg gagacgaacg aaaaatgaat cagcacgtaa aagtaacatt tgattttact
     5161 aattataatt acggcgcata tgacttagca gtaccagcat atttaccgat aaaaaattta
     5221 atagctttag tattggatag tttggacatt tcaatatttg atgtcaatac acaaattaaa
     5281 gtgatgacga aaggtcaatt acttgttgaa aacgaccgac ttattgatta tcaaatcgct
     5341 gatggagata ttttgaagtt actataggag gaaaaataga tggttaaaaa tcatgaccct
     5401 aaaaatgaaa tgcaagatat gttaacacct ttagatgctg aagaagcagc taaaacaaaa
     5461 ttacgcttag atatgagaga gattcctaag tcttcaatta aaccagaaca ttttcattta
     5521 atgtacttat tagaacaaca ttctccatat tttatagatg ctgaattaac tgaactacgt
     5581 gatagtttcc aaatacatta tgacattaat gacaatcata caccttttga taatattaaa
     5641 tcatttacta aaaatgaaaa attacgttac ttactcaata tcaaaaattt agaagaagta
     5701 aatcgtacac gttacacatt tgtgttggca ccagatgaat tatttttcac aagagatgga
     5761 ttaccaattg ctaaaacaag aggtttacaa aatgttgttg atccattacc ggtgtcagaa
     5821 gctgaatttt taacaagata taaagcactg gttatctgtg cattcaatga gaaacaatca
     5881 tttgatgctt tagttgaagg aaacttagaa ctacataaag gaacgccatt tgaaactaaa
     5941 gttattgaag cggcaacgtt agatttacta acggcatttt tagatgaaca gtatcagaaa
     6001 caagaacaag attatagtca aaattatgca tatgtacgca aagtaggaca taccgttttc
     6061 aaatgggttg ctatcggtat gacaacgtta agtgttttat taattgcatt tttagccttt
     6121 ttatattttt cagtaatgaa gcataatgag cgtattgaaa aaggatacca agcatttgta
     6181 aaggaagatt atacgcaagt actaaatacg tatgatgatt tagatggtaa aaaattagat
     6241 aaagaagcac tttacattta tgccaaaagt tatatccaaa caaataaaca aggtttagaa
     6301 aaagataaga aagaaaattt acttaataac gtgacaccaa attcaaacaa agactactta
     6361 ttatattgga tggaattagg acaaggacat cttgatgaag cgattaatat tgccacttat
     6421 ttagatgata acgatattac aaagttagcg ttgattaata agttaaatga gattaaaaat
     6481 aacggagatt tatcgaatga taaacgttct gaagaaacga aaaagtataa cgataaattg
     6541 caagatattt tagacaaaga aaaacaagtt aaagatgaaa aagcgaaatc tgaagaagag
     6601 aaagcaaaag cgaaagatga gaaattaaag caacaagaag agaacgaaaa gaaacaaaaa
     6661 gaacaagcac aaaaagataa agaaaaacgc caagaagctg aaagaaaaaa atagtatagg
     6721 actgaggcaa agacaatgca taaattgatt ataaaatata acaaacaatt gaagatgctc
     6781 aatttgcgag atggtaagac atatactatt agcgaagacg agcgtgcaga tattacgttg
     6841 aaatcgttag gcgaagtcat tcatttagaa caaaataatc aaggtacttg gcaagcgaat
     6901 catacttcta ttaataaggt gcttgttaga aaaggtgacc ttgatgacat tacattacag
     6961 atttatacag aagctgatta tgcatcattt gcgtaccctt cgattcaaga tacgatgacg
     7021 attggatcaa atgcatatga tgacatggtc attcaaagtt tgatgaatgc catcattatt
     7081 aaagattttc aatcgataca agaaactcaa tatgtgcgca tcgtgcacga taaaaataca
     7141 gatgtgtata ttaactatga actacaagag caattaacga acaaagctta cattggtgat
     7201 catatttatg ttgaagggat atggctcgaa gtacaagctg atggtttaaa tgtattgagt
     7261 cagaatacag tggcatcgtc attaattcgc ttaacacaag agatgccaca tgcacaggca
     7321 gatgattaca atacgtacca tcgttcgcca aggattattc accgtgaacc gaccgatgat
     7381 attaagattg aaagaccacc acaaccaata cagaagaaca atacagtgat atggcgttcc
     7441 attataccgc cattagtaat gattgcttta actgttgtca tctttttagt gagaccaatt
     7501 ggtatttata ttttaatgat gattggtatg agtacagtaa cgatagtatt tggtattaca
     7561 acgtatttct ctgaaaagaa aaagtataac aaagatgttg aaaaacgaga gaaagattac
     7621 aaagcttatt tggataataa atctaaagag attaataaag cgattaaagc acaacgtttt
     7681 agtttgaatt atcattatcc aacggttgct gaaattaaag atatcgttga aacgaaagca
     7741 ccaagaatat atgaaaaaac atcgcatcat cacgatttct tacattataa gttaggtatt
     7801 gcgaatgtag aaaagtcatt caaattagat taccaagaag aagaatttaa ccaacgtcgt
     7861 gatgaactat tcgacgatgc taaagaattg tatgaatttt acacagatgt agaacaagca
     7921 ccattaatca atgatttaaa tcatggacca attgcatata ttggtgcacg acatctcatt
     7981 ttagaagaat tggagaaaat gctaatccaa ttgtcaacgt tccatagtta tcatgattta
     8041 gagtttctat ttgtgacacg tgaagatgaa gttgaaacat tgaaatgggc acgttggttg
     8101 ccacatatga cattgagagg tcaaaacatt agagggtttg tttacaatca acgaacacgt
     8161 gaccaaattt taacgtcaat ttatagcatg attaaagaac gtatccaagc tgtgcgtgaa
     8221 cgcagcagaa gtaatgagca aattattttc acaccgcaat tagtgtttgt cattacagat
     8281 atgtcattaa ttattgatca tgtcatttta gaatatgtaa accaagattt atcagaatat
     8341 ggtatttcat taatctttgt tgaagatgtg attgaaagtt tgccagagca tgtagatacc
     8401 attattgata tcaagtctcg tactgaaggc gaactgatta cgaaagaaaa agaattagtt
     8461 caattgaaat ttacacctga aaatattgat aacgttgata aagaatatat cgcgagacgt
     8521 ttggcgaatt tgatacacgt cgaacatttg aaaaatgcaa ttcctgatag tattacattt
     8581 ttagagatgt ataacgtgaa agaagtagat cagcttgatg tggttaatcg atggagacaa
     8641 aacgaaacat acaaaacgat ggcagtacct ttaggtgtaa gaggtaaaga tgacatttta
     8701 tcattgaact tacatgaaaa agcacacggg ccacatggtt tagttgctgg taccactggt
     8761 tcagggaaat ctgagattat tcaatcatac attttatctt tagctattaa ttttcaccct
     8821 catgaagttg cattcctatt gattgactat aaaggtgggg gtatggcgaa cttatttaaa
     8881 gatttagtcc atttagttgg tacgattaca aacttagatg gcgatgaagc gatgcgtgcc
     8941 ttaacatcaa tcaaagccga attgagaaaa cgtcaacgtt tattcggaga gcatgatgtt
     9001 aaccatatta atcaatacca taagttattt aaagaaggtg ttgcgacaga accaatgcca
     9061 catttattca ttatttccga tgagtttgca gaattaaaat cagaacaacc tgattttatg
     9121 aaagaacttg tatcaacggc acgtattgga cgttcgttag gtattcattt aatacttgcg
     9181 acacaaaaac catcgggtgt tgttgatgac caaatttggt ctaactctaa atttaaattg
     9241 gcattaaaag tacaagatag acaagacagt aatgaaattt taaaaacacc agttgcagca
     9301 gacattacat taccaggtcg tgcgtattta caagttggta ataatgaaat ttatgaatta
     9361 ttccaatctg catggagtgg tgcaacatat gacattgaag gcgataaatt agaagttgaa
     9421 gataagacga tttacatgat taatgactat ggtcaacttc aagcaatcaa caaagacttg
     9481 agtggacttg aagatgaaga aacaaaagaa aatcaaactg agttagaagc ggttatcgat
     9541 catatcgaat ctattacaac acgattagaa attgaagaag ttaagcgtcc atggttacca
     9601 ccattaccag aaaatgtata tcaagaagat ttagtagaaa cagatttcag aaaattatgg
     9661 tcagatgatg caaaagaagt ggaattaaca ttaggactta aagacgtacc agaagaacaa
     9721 tatcaaggac cgatggtatt gcaattgaaa aaagctgggc acatcgcgtt aatcggaagt
     9781 ccaggatatg gtagaacaac gttcttacac aacattattt tcgatgttgc aagacaccat
     9841 cgtcctgatc aagcacatat gtacttgttc gatttcggta ccaatggttt gatgccagtc
     9901 acagatatac cacatgtcgc tgattacttt acagtagatc aagaagacaa gattgcgaag
     9961 gcgatacgga agatacatga tattatttct gagcgaaaaa gactattaag tcaagagaga
    10021 gtagttaata tagagcaata taataaagaa actggaaatt caattccaaa tgttttctta
    10081 attatcgata actacgacac ggtgaaagaa tcaccattta tggaagaata tgaagaaatg
    10141 atgtctaagg taacacgtga aggtttagcg ttaggggtat acatcattct ttcaggatca
    10201 agatcaagtg caataaaatc agctatattt actaacatta aaacaagagt tgcgctgtac
    10261 ctctttgaaa acaatgaatt aacaaatatt attggttctt ataaaaaagg tgtgaaagat
    10321 gttaaaggta gagctgcaat aaatgatgat aacttcacac agttccaaat tgcgcaacca
    10381 tttgaattag cagaaggaca aacatataac gagcgcatta aaaatgaagt agcacaaatg
    10441 aaagaattct atgtaggaga ttatccaaaa catattccaa tgatgccaga taaggtattt
    10501 atggaagata ttcgagaagc atatgatttg gaaaaaatca ttcatgaaga acacaaatta
    10561 ccattaggat tagactttga ggatgtggaa ctagttagtt tagatctgac aagttcttcg
    10621 attgtaacag caattaaacc aactgagatg gaaaaaatga atgatgttat aatgtctagt
    10681 ttatcagtat atagtaaaaa ccaatttgtt atcttggttg atgcagaaga taatatgagc
    10741 cagtattcag aagatgtaac ctcttattat tcagcaccat cagatttgag caacattaga
    10801 ttaggattta agcaagaaat agaagcaagg aaaaatggcg agaaaagtat agaggaatgc
    10861 aagattgtat ttataaataa tattaaacga ttcaatcaat taactggaat gacagaagat
    10921 gaaattcgtg tgttatttaa tgaaggtcaa aaagttaata ttatcattat tgcaagtgga
    10981 ctatactcag atacaattgg tgcatttgat agagaaagta aaatgatggt aagaactata
    11041 aatcaagctt tgattagtca taagatatcg gaacaagaat ttataagagt taaagatcga
    11101 tttggtgaac cagaattaaa agtgggagaa atgtattaca ttaataatca agaatatcaa
    11161 aaaataaaac taatggaggg atagagttga taaagttaaa ccaagcctct gtcagcaaag
    11221 aaatttcaag cattcgaaca aatggacaag gcttaaaaca atcaaatggg aatgtaaatt
    11281 taagtaaaac gaatctcgtt acttttaaag aatatgtaaa tatgtttgaa gattatcaat
    11341 cagctctatc taattatgaa aacataattg aacaagatac gacggctatg gatacaacgg
    11401 taacagaaat tgttgaaaac gatagagaaa tagcaggaca aattaataaa taattaaaca
    11461 agacgggtga gaaaaatgag taataaggga gaaataagaa ggcaaattgc taataaagaa
    11521 agagaaaaag caagtaaaga agcacaacta acagatttaa aagaggattt aagaaggttg
    11581 aaagacgctt cgaaaaaatt ggatacagca ggagaagatt ttaacaaagg acagagtagt
    11641 tataacaaag tagaaattag tacttcagat tggaaaggag aaagaaggac taaaagtgac
    11701 agcaaaaaga aagatgtcga ttctgaatta aaaaaagttg aacaagactt tgatgatgcg
    11761 aagaaggcta ttaaaaaaga tattcaggat aaagaagaag aaatcaaggg agtcgaagga
    11821 gaaatcagta ctataaatgc agctatagat gcacttaaat caaaattata ataatagaga
    11881 gggaaattta ttatggggta caaagttgat atgtctgaag tgcacaatat gcaaaagtct
    11941 attgattcaa gcttaagtgc aataaataca aaggttagca cgcttagcag tagcataaac
    12001 aaccttataa atactgaagg attcgaaggt cagacagcaa gtagtgtcaa aaactatagt
    12061 aagacatttc atttacaatc aataaaaaag attgaaacaa taaacaaaga ttttaaatca
    12121 gatattgcga aatctatacg taaattccaa agtgaagttg ataataatgc ttcagctata
    12181 ttggatgaag acgaaataaa aaaatacaaa aaagatattg atgatgcatt aaaagatgtt
    12241 ttcaaatctt ctaaagatgc aaacggagct atttcagatg tttcagattt aacaacagcc
    12301 aaaaaaataa agacagaaaa cttggccaat aaaatgggag attttaataa agatattgat
    12361 caaacggtgg aaagattaac gaccttcgac gccaacaatt ctatagatgg tgataagaca
    12421 gataacttaa taactgaatt aagtggtgta aattcatatg tcaaaaacat gaagccaaac
    12481 cgtgcaagaa ttagttctac aagtggaaaa attgaaggtg caattgcaag gcataaaaca
    12541 agtgaagagt tagtaagatg gcaaacttac atggaaacgt taagtgaaaa tctttataaa
    12601 acaccaggtc taagtaaagc agcttctgac ataattaccc aagctggacg cgagtactat
    12661 gctatcaaag ctgtagggaa tggcagtgct ttaagaggat ataaagaata tctgaaaaca
    12721 cgagatatta ataaattaat taacaaaatg aataaaaacc aattaaaaag aatgtcattg
    12781 gtattaaata cagatagagg taatattaaa ataaaaaatg cagtaaaaat ggcaagtgaa
    12841 tttgcccgta ataatccttt taaaaaagga aatttagtaa attggatgag caaagtccaa
    12901 gggtatcaaa gtgtaagtgc tcaaatatta aaaaaggggt tgcaagataa gaactttaaa
    12961 tatacatttg gagatgcaaa gaaatttttt gatacagctg atatgaaaaa agcagcagtc
    13021 acagaattta aaaatacctt tgtaccacaa aatataagag aaaccttctt taagaaagaa
    13081 aatttaacat ctaagacagc attaaagaaa aatatgagta aattctggaa tgaagatatt
    13141 aagggtggaa tcaaagagtt taaattagac tttgctaaca aaaatatcat tggtaaactc
    13201 ggcaaattgt tgaaactagg aggcaaggct ttaaaaccat taggatttat tactgcaatt
    13261 actgacaaca taggaaagaa atcttggcaa gaaaaattag ttggtatggg ggtagattta
    13321 agtgctattg gtgcaagtgc agctgcaggt gcagctgttg gaacggcaat accattacct
    13381 gtagtaggaa cagttgcagg tgcattagct ggagcagcag tgggtatagc tttagattat
    13441 aaaataccgt atcttgataa gtctgcaaca gaattagcta aagatggtat taactcgggt
    13501 attaacaaag ttaaaagtgg atttggaaaa gtaacttcag gatttaaaac agttttcgga
    13561 taagaaaggg gcagacaaat gaaaatacaa gagattgtag gaaatataaa gagagcttat
    13621 ctgacaccat taataatttt tgtgattttg ttttcattat tttttgattt gataatgtat
    13681 ttttatggaa accttcaagg gaaattgcca atatatttag atgtatttct aatatttgca
    13741 gcagttgtat ttattttaat gtacttccaa gagaaaagtg gagaagttaa ggttgaaaaa
    13801 tcaaacgtta ttcgttattt aacattaaat gttgtagctg ggtattcaat ggtactactt
    13861 gttgcctcta tttatgtttt tggggtagca ggctatggtt ttgatgtatt taattactgg
    13921 ttaggtatta tattaatgct atttgtatcg tggtttgctc tatttttgtt ttataaaaat
    13981 gaatttgata gtgaaaatcc aaataaaaca gttaatgtta tagcaattat tataaagtta
    14041 tctgcttttg gaggactttt ctatatcaga acagtcgttc ctaatactgc agatgaagag
    14101 aaatttataa ctctaagtat cttaattaat atagctgttg atttattgct ggttagatca
    14161 tattttaact atgcattata taagagtgta aaaaaagata tcgaaaacgg agtggatgat
    14221 taatggaagt aggaatgtta attatgccgt tttgtgtgaa gattacattt gaaattgata
    14281 aaaatgataa ttggttaaat gcattagata cagtaaatga atttttcatg gacaaagata
    14341 tatatccaac tggtcctata attttccaaa gagaattagc aggtttgggt gaatataaat
    14401 atacagcata tatagctctt aatgatgaac ttcaagatat acctgaattg aacatcaaat
    14461 atattgattg tttagaggta ggacctacgt tatcaactaa gtgttttgaa gaagaggaat
    14521 ttgaaagagc ttataaagaa attgaatatg tagctaacga aaataatatt aagatcctaa
    14581 atcaaccata ttatcatgta atggtagatt attttggtgg tacggctttt gaaatttatg
    14641 ctcaagtaga tttggatgag agtgaagtat atggataaaa atggatactc atatattatg
    14701 atgaaaaatg tagcttacaa aacttatttt gatgtgacta tggatgacta tgaagatata
    14761 ttaaaagact ttacaaatct tgcaatttct aaaggtaaaa caattacagg gccattaacc
    14821 tttgcagtca cgcaaataga cttgcaaaaa aggatgaata ttgatttatt tatttcagtt
    14881 ggaaaatgtt ttaaatcaaa tgaagaacta tcatatagaa catatttttg tctagataac
    14941 atgttacatg gtcgtatcac ttccaacaat tttataacag atgaaatagc gttacttgag
    15001 gatatgaacg aatttgcttc tgacaacaat ttaacgttta cttcgcctta ttatcacaca
    15061 atgagaaaaa gttttagcgg tgaacaaggt tggattgatg taaaggccaa agtgtatgaa
    15121 aatgattaat taataatcag tagaggtaat atatgataat ttttatttta ttaacagttt
    15181 ttgcactctt ttatatagcg atgattgcta gtttatttaa aagtgaaggt ttttcaataa
    15241 taggcttaat attggatata gttattttga caacgttaat attttattat tttgtaggtg
    15301 cgagttttgt tgataatgat ttaagtaact ttttagcatt catgaatttt gggtcatttg
    15361 tgtatatgta ctatgcaatt aagagcttgt gggtgaagcc taagttagta aattatataa
    15421 ttgcaaaaga aataggcgaa tcaaaagatg tcattgaaga acaggaatta gatttacaga
    15481 catcaaagat aagaggtatc tactttttca ttattgcaat tgccttgtta ataattacta
    15541 aattaagaat gcaacctgag ttacaggcag atgctatatc gatgaatcca gtttttattt
    15601 ttataggagt tattatcatt ttaatttggc tagtactgga tatatatcgt aagaaaaaat
    15661 acggtatatt cttattcaag acgatagtgc cattagttgt tacgacttgg attattatag
    15721 ctactattgt actttcataa gaaattttca ataatttttt tggttataat tgttgatgga
    15781 tattaattaa tcgatagaaa aatcttaatg atttttg
//
LOCUS       DAKSQE010000062        15548 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.062, whole genome shotgun sequence.
ACCESSION   DAKSQE010000062 DAKSQE010000000
VERSION     DAKSQE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15548
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.062"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            218..1303
                     /locus_tag="O0V33_001657"
     CDS             218..1303
                     /locus_tag="O0V33_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCX8976286.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPAAILGIIDSPTT
                     FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGDLGGFTLVLIAVPLY
                     AVLKTVVSNIFKYRQRIIDKANSNVKD"
     gene            complement(1499..2269)
                     /gene="fabI"
                     /locus_tag="O0V33_001658"
     CDS             complement(1499..2269)
                     /gene="fabI"
                     /locus_tag="O0V33_001658"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCX8976287.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(2547..4391)
                     /locus_tag="O0V33_001659"
     CDS             complement(2547..4391)
                     /locus_tag="O0V33_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HCX8976288.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNDDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIISKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKTHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
     gene            complement(4401..5786)
                     /gene="mgtE"
                     /locus_tag="O0V33_001660"
     CDS             complement(4401..5786)
                     /gene="mgtE"
                     /locus_tag="O0V33_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCX8976289.1"
                     /translation="MSMNTDEKERVQEELYDETLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQDHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            complement(5807..6661)
                     /locus_tag="O0V33_001661"
     CDS             complement(5807..6661)
                     /locus_tag="O0V33_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCX8976290.1"
                     /translation="MKFKYHISQQETVKTFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMPNDILEIHLPREIPSVNLIPYARKLEVLYEDAFVIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQEHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKVNLKKIYTC
                     LVYGKTHTFGIIEANIRRSKDSIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            complement(6658..7467)
                     /locus_tag="O0V33_001662"
     CDS             complement(6658..7467)
                     /locus_tag="O0V33_001662"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCX8976291.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            complement(7484..8119)
                     /locus_tag="O0V33_001663"
     CDS             complement(7484..8119)
                     /locus_tag="O0V33_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCX8976292.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            complement(8136..8483)
                     /locus_tag="O0V33_001664"
     CDS             complement(8136..8483)
                     /locus_tag="O0V33_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976293.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHENMTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            8669..9262
                     /locus_tag="O0V33_001665"
     CDS             8669..9262
                     /locus_tag="O0V33_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCX8976294.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQVNYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTTDYELEFEVKDYNQG
                     LEKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
     gene            9366..9731
                     /locus_tag="O0V33_001666"
     CDS             9366..9731
                     /locus_tag="O0V33_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HCX8976295.1"
                     /translation="MTTTPYDIIGKEALYDMIDYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHMAFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            9754..10560
                     /gene="yjbH"
                     /locus_tag="O0V33_001667"
     CDS             9754..10560
                     /gene="yjbH"
                     /locus_tag="O0V33_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HCX8976296.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRLRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(11020..12828)
                     /gene="pepF"
                     /locus_tag="O0V33_001668"
     CDS             complement(11020..12828)
                     /gene="pepF"
                     /locus_tag="O0V33_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCX8976297.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDENAHPLTQGTFIKYLESDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
     gene            complement(12876..13862)
                     /locus_tag="O0V33_001669"
     CDS             complement(12876..13862)
                     /locus_tag="O0V33_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCX8976298.1"
                     /translation="MLVALNEEKERVLATTALRKTQYFCPVCGKQVILKRGLKVISHF
                     AHKHLAEQKCFNNESIKHYKSKLILAQMIQQQGCKVEIEPFLKEIKQIPDILINNKYV
                     IELQYSPIPYKQILQRTEGLKKMGYKVSWLLNDVDYCHNKVKFNHFQSMFINPITRKL
                     HTFNLEKKQIIMFQQIQYLGGHKYVAEKKNAKISELFNEAPCDYHAVYKLSKFAINQY
                     IKYCRWQNSVLEPTLSAMYQLQLTDHEVVHNYGYIFPEQIYIENHPIEWQLQVDLWLK
                     NGKSKLVSDNLNYFKLKKFIVALESKTAIIEKLINNYLNISSDRGNDVQILF"
     gene            complement(13983..14702)
                     /gene="mecA"
                     /locus_tag="O0V33_001670"
     CDS             complement(13983..14702)
                     /gene="mecA"
                     /locus_tag="O0V33_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCX8976299.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVHFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
     gene            complement(15073..15468)
                     /gene="spxA"
                     /locus_tag="O0V33_001671"
     CDS             complement(15073..15468)
                     /gene="spxA"
                     /locus_tag="O0V33_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCX8976300.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
BASE COUNT         5194 a         2721 c         2135 g         5498 t
ORIGIN      
        1 attaaaataa gttattcata taaattggta ctgtttgatc catttgcaga cttacgagtc
       61 attgaaaagt ctcacaaact atcgaaagta aatatcttaa aatagaaagt aaatggttaa
      121 tttaagtata tttaaaaaat attaaccttt ttaagcactg ctatttagga tatactaaat
      181 aataactaag tttagaaaaa taggaggaac atcgtttatg ttaaacaagg tttggttccg
      241 aactggcatc gctctgatta tgctgttcat tctcatcaaa ctatttatgg aagtgcacga
      301 agtatttact ccaatagcta caatcatcgg ttctgtattt cttccatttt taattagtgg
      361 ttttttattt tatatctgtc taccttttca aaacttactt gaaaaagtcg gttttccaag
      421 atgggctagt ataacaacaa tcatgttagc tttgtttgct attatcggct taattgttgc
      481 gtttgttgca ccaatcatca tttcaaatat caacaattta attagtcaaa cacctgcctt
      541 acaaaaagaa gcagagcaaa ttattaaatt cgcactagct caaatggata aattacctga
      601 agatgtaaca agtagaatta caaatatggt taaatcaatg ggtgatggcg ttactaatat
      661 tttatctaat tcattacagt acattacatc attaatttca accatcttct tattgattat
      721 ggttccattc ttcttaattt atatgttaaa agaccatgaa aaatttatcc cagctgttgc
      781 taaatttttc aaaggtgaac gtaaagtatt tttcgtagat ttattaactg atttaaactt
      841 tacattaaaa tcttacattc aaggtcaagt aactgtcagt gttattttag gtatattctt
      901 atatatcggt tattcaatta ttgacttacc atatattcct ttattagtac tatttgctgg
      961 tgtcgcaaac ttaattcctt tcttaggttc atggttatca tttgcacctg cagcaatact
     1021 cggtattata gatagtccta ctacatttat atgggtttgt atcattacat taattgcaca
     1081 ccaacttgaa ggtaatatca ttacaccaaa tgtaatgggt aaatctttaa gtatccatcc
     1141 tttaacaatt atcgttgtta ttttagcagc aggagattta ggtggcttta cattagttct
     1201 gattgcagtg ccattatatg ctgtacttaa aacggttgtt agtaatattt ttaaataccg
     1261 ccaacgcatt attgataaag caaacagtaa tgttaaggac taattctgtg gatgtctttt
     1321 aataatataa gatactatcg cctcaaaagt tgaaactaca gcttttgagg cgattttttt
     1381 gtgcataaaa aaatcagtca gatgaaatat caaataattt tccatcagtc cgattattat
     1441 aaaagcaaaa aagctttgct cacatatata ataacgtgaa caaagctgtt gaatgatatt
     1501 atttaattgc gtggaatccg ctatctacat gaatattttc acctgtaacg ccacttgata
     1561 aatcacttaa taagtaagcc gctgttttac ctacttctac ttgatcaaca ttacgtttta
     1621 aaggtgcacg ctcttcgatt tctttaagaa ttgtattgaa accacccaca ccttttgcac
     1681 ttaatgtacg gattggacca gctgaaattg cattaacgcg aatattatct ggacctaagt
     1741 ctaatgctaa atatttaaca tttgcttcta aactcgcttt agcaacaccc atcacattat
     1801 agttttgaac tgcgaattcg ccacctaaat atgttgttgc aacaatgcta ccaccttctg
     1861 gcattaattt tttagcttca tgagcaacga tagttagtga gtaagaacta atgtcttgag
     1921 ctaacaagaa accttcacgt gaagtttcag aaaaacgtcc gcgtaagtct tccatattag
     1981 caaatgcgat tgaatgatat acaccatcga tattgccaac atctttacca atttgctcaa
     2041 aaccattaat aacttcttca tcgctttgaa catcaatttg atataagtgc gcttctggtt
     2101 gatttaattg ttctaataat ttttcaagct ctttacggct acgttcttta cggtaagtaa
     2161 atactaattt agcacctaat tgatctaaaa ctttagcgac accaaaagca atactacgct
     2221 tattagcgat tcccatgatg acatatgttt tgttttcaag atttaacatg taagataact
     2281 ccttataata attaatttaa cggatagaat atctaatccc agttttaact aactttttat
     2341 tcgtcaatac attttaacac tattatgact ttttaataaa gttatttaaa cgtattacaa
     2401 cgataaatgt ttgatttagt cctttctgta tcaaacttga tttacgtaaa aagcatgtat
     2461 ttatcaaaat catatacata caagcatttt aaaatcatta attttacgtc gtactaaaat
     2521 acgtctgcat tttaatatca ttattgttaa aaataaaatt ctaactcttg cttcaattca
     2581 tcaacgtatt ctttagcacc ggtaacaatt aagcgatcac catatcgcaa ttgtgtatct
     2641 ccatgcggaa caatcgactc attattacgg ataatacgca cgaagatgat gtctcctccg
     2701 aatgggaaat tacgtaattg aatattttca tatttataat ttaacatttg aatttcatat
     2761 agtgacgttt caacattact taataaattc aacatgttag gtgtttcaat taaacctttt
     2821 aataagattt tattacttaa gtagctactg aaaatttcaa tacctgaatc aactaactct
     2881 gtatcgtccg ttgtgctttc aagtctgcaa atgacacgct caacttgatg tgttttggct
     2941 aatttagcaa cttttcggtt aatatcatcg tcattcgtgg cacaaacaac tatgtctcgg
     3001 tcaaacagac ctagtcgttc taaaacatct tgttcataat cagcaatttc tatcatcgtg
     3061 atatcatccg acaattgacg acgatcactc aagtctttgc gataatataa tgtcacgtca
     3121 tataactgag atgttaaatt ttgcgctata ggaatcgtta attgattttt accaattaaa
     3181 ctaacttcaa ttttacggtt aaactcatct ggaactggaa acagtttttt gaaaataatc
     3241 ggaacgaata cacatgtaat gactgcgctt aaaattaata tacctgacgt ttcagctgaa
     3301 atagcattta atctttctga aattttggct gcagctatca cgagcgataa tgttgatgtt
     3361 aataaaaatg ctgatgcaat cgttgttttc atatcaaacc aacgtcgaat aaacatgact
     3421 ggaattaatt ttgaaatgat aaatgcaacg attaaaatcg gtatgataat tagtaatttc
     3481 ggttctttaa ttaatgaagg tatgtttaaa tctacaccaa ccattatgaa gaaaatagga
     3541 ataaaaaacc cgtaaccaaa tgagtctaac ttttcaacca tttcttcatc tggatttaat
     3601 aatgaaacaa cgacacctgc taagaatgca cctaatatat tttctgcgcc aactccctct
     3661 gctagggcta ctaataatat tattaatgca aacaccgcac gaataccgat ttgcgtcgta
     3721 ccatccatca atttttgtaa aaatgacatt cttttaaatt gaacacctaa aatatatgaa
     3781 attgctgtga aaacaactaa tatacctatt aaccatattg tactgccgcc ttgaccattg
     3841 attgcaccat agaccgttaa taaaatcata gttactaaat ccgcaagcac tgctactaat
     3901 aggataaatt gccctatagt tgttctcata atattcattt cttttaaagt tggaacaact
     3961 acgcctaagg aaatagttga aatgataatg accattaata acacatcatc cactaatcca
     4021 agccatttaa atacatacgc taaaagaatc gaaataatca taataaatgc aaatacagtt
     4081 aacgctagat taagatgccc tggaattgag gattcatcat cattttgtcc ttgacgtgcg
     4141 cgtttatctt ttttaaaagc tttaaaatca atttctaaac cacttaaaaa cattaaaaag
     4201 ataaagccta acgttgataa aatatttaga attgaatccc tttctactat atttagaaat
     4261 gaatttccaa tcacaattcc catcaaaatt tctgcaacaa caactggcaa gaaattaata
     4321 tttaatcgat tgacaattat tggagttaaa aacgctgcta aaacaacaat aactaaagat
     4381 aaaaactcca taactcctcc ttaaattaag taagccataa atgatgtagc taaaccaaaa
     4441 taaatcaaca tactaataat atcattaatt gttgtaataa atggtccact agccactgct
     4501 ggatcgatat tcaatttatt catcaataat ggaatcatcg aacctacaaa cgtccccacc
     4561 gtcatcgcac aagttaaact tcctgcaaca attaatgcta aaagtggctg atgatatatt
     4621 gcaacaataa ttgtaaataa tattgttgaa catacaacac ccgataatac accacttccc
     4681 gcttctctta atgcaattct aaatttactt tgctcattaa tttcccctgt cgtaatgtta
     4741 cgaactgaaa cggctaaaga ttgtgtacct gaatttcctg acataccact aataatagga
     4801 ataaacgctg cgagtagcgc tacattttct aatgtttttt cgaatctccc taaaattgtc
     4861 gcagtaatca ttcctaaaaa tgttaaaata atcaaccatg gtaaacgctt taatgctgtt
     4921 ttaatgattg aatcattagt cgaatcgata tctgaaaccc cggctaaacg agagtagtct
     4981 tcactagcct cttcatccat aacgtctaaa atatcatcaa tcgtgatgat ccccagcaaa
     5041 tggtcttggt aatctataac aggtacagcc atgaaatcat agtctctcat aacttgagca
     5101 acatcttctt ggtcgtctgc tacattcaca ctaatgacac gttcgttcat aatatcttca
     5161 atatacgcat cattttcagc tacaattaaa tctcttagcg ataaaacacc tactaattta
     5221 ccatcatcat caacaacaaa gataacatat attgtttctg cgtctggcgc ttgtgctttg
     5281 accaataata acgcttcttt aacaggcgta tgcgctttaa gtgataaata ctccgtcgtc
     5341 ataataccgc cggccgtatc ctcatcatag tgaagtaatg ctttgatttc attcgcgtca
     5401 tctttattca tcaatgttaa aagactagca acttttggtt tagtcaactc atttaaaata
     5461 tctactgcat tatcgtatga catttcttct aatatgtgac tagcgtatgt cgcattcatc
     5521 ttatcaaata gcaactcata ttcatcgtca tcaatatcta attgatcaaa gaaatttgca
     5581 acctcttcag gtgataaata ttgaaaaatc ttttgtctat tttcatcggt agtatcttca
     5641 aaatactcac tttgttcata tgtgtgtaat gctagaaatt catctctaaa ttgatcaata
     5701 tcatcatttt ctaagtattg atctaataat gtctcatcat ataattcctc ttgaacacgc
     5761 tctttttcat ctgtgttcat tgacatactt acaccccctg tgtgaattag agttgattga
     5821 ataatttaag taattgctta taatcaattg taatataaat attgttctta ttgattggat
     5881 gaacaaaata tatttgcgta cattgcagta cttgaccatg aattttgtca tgaaaaccat
     5941 catacaaaga atctcccaca attggatgcc caatatgttg aaaatgtaca cgaatttgat
     6001 gtgtacgtcc cgtatgcaaa tgaactttac acaaactata tttatcattt tgatttatta
     6061 cttcataaga tgttttagcg tatttaccat ccgaggcaac ttctctagtt ataatactat
     6121 cctttgaccg tctaatatta gcttcaataa taccaaatgt atgggtttta ccatatacaa
     6181 ggcaagtata tatttttttc aagtttactt tagaaaataa atgatggatg tgtccatatt
     6241 tagcgaatat cacaatacca gttgtattac gatctagacg cgtaacaata tgtgggttaa
     6301 tattttcacc atgttcttga caatgatata gtacttgttc gattaaactt tcatgaggat
     6361 gttctctcga aggcgtacaa ttttgattgt ttggtttagt aactatgatg acaaaagcat
     6421 cttcatacaa tacttctagc ttacgagcat aaggtattaa attaacactc ggtatttctc
     6481 gcggcaaatg aatttctaat atatcatttg gcattaattg cttacgcact gtgactggtt
     6541 catcattaac aattaaagcg ccattatttt taatggcgct cactgtcttc ttagaaaaat
     6601 catgtcgtgc taaaaaagtt ttaacagttt cttgttgtga tatatgatac ttaaatttca
     6661 tcgttcttca tcacttgata tgaaagaatc gtgtacacgt ttccagaatg ggaatggtct
     6721 aaaacgtgca aacctcactt tttcatttgc tacacggtat tgtattgaat taacattttt
     6781 atgtttgata ctaacatgat ctatcgtcat tctaatggta tcatgattaa ctggtgatat
     6841 taaacatgta tgatgctttg gtaatacaag tggtgatcct accgttctaa acacacgatt
     6901 atttattgag gcaatttctg taatttgcat tgcttcaagt gaaggatgta taagtgcgcc
     6961 acctagcgct ttgttataag ccgttgaacc cgaaggtgtt gatacacata atccatcgcc
     7021 tctaaatcgc tcaaagtgtt tccctcttaa gttaacatcc acaacaagtg ttgagccatt
     7081 ttcagttttc atcgttgctt catttaatgc taaatacctt gtttcatagc cgttgtcgtt
     7141 gtatctcata ataatttcaa gcaatggata ttcaatgacc tgaaactctg aattattaat
     7201 ttcgatgatt aatttttcaa cttcatgagg taaccaatcc gcataaaatc ctaaatgacc
     7261 tgtatgaaca ccaacaaatg ccacttttga taacatgtgg ctatactgat ggaatgcttg
     7321 tagtaatgta ccatcaccac caactgaaat aacaatttca ggattttcac tatcctcaat
     7381 catgcgaaaa tctttcatat agttcatcat tttatgcttt aaggcattag acttggagtc
     7441 acctttagtt aaaattgtat agcgcatgat ttataacacc tcgttaatca ttttcatgtt
     7501 ttttagaacg tttttgcgtg taatattttt gagcttcctg aatttcatct ttaatttcag
     7561 acatctcttc atcaagtaaa tacgctgctt ctgccgctct ttccaaacga tgttgaattt
     7621 catccggata agcaccatca tatttatatc gtaaagtatg ttcaatcgtt gcccagaaat
     7681 tcattgctaa tgtacgaatc tgaatctcag ccaatataaa cttttggcct tgtaatgttt
     7741 cgattggata ttcaataatg acatgatacg agcggtaacc actttcttta gtgttacgaa
     7801 tataatctcg ttcttcaatt actttaaaat cttttctttg tcttaaaata ttgacgacaa
     7861 catcaatatc ttcaacaaat tggcacatca ttcttaaacc agcgatatcg tacatttctt
     7921 ctcttaacct atcaaatggt atttgtcgtt tgtttgcctt atctataata ctagcgattg
     7981 gtttaacacg accagtaaca aattctattg gcgacgcttg ttcaccaact tcatattgtt
     8041 tgcgcatgcc tttaagtttc actttcaact catcaaccgc ttgcttataa ggtgttaaga
     8101 actgatccca ttgattcata gtgcttcacc ccgctttatt caatgtcgaa tgtttcttca
     8161 acggttgcaa caaattcctt accataatta ttatttcctt ttatgttgtc taaaatataa
     8221 gtaagttctt ggtggaattc agtcaattct agttcatcat tgataataat ttttttagtc
     8281 atgttttcat gcaacatcgt ccatgtttct tgtacgaaaa tgagataagc atacgcttta
     8341 ccatcatcta atgtatacgc aaaggcataa ttatcactat caacgagcat ttgtcctact
     8401 tcagataatc ctttaataga atcttctgta taacaataaa gtatgtcatc tttaatttta
     8461 atttcattaa tatataaacg catgcttaat cctcctctta ttccataaca ttttaacaca
     8521 attcaataat acaaaacact tacgaacact atttttaatg gtcaaattaa cattgataat
     8581 agataaagga aattactttt aaaattcaac tttgtcattc ataatatagt taagataaat
     8641 tgaatttgat acgaggtgta tacatatcat ggcaacaaat catgaaatag aatttaaaca
     8701 aatgataact gcatcaatct ataataaatt gcaagagaaa tattttaaag acagtgtgct
     8761 ttttaagcaa gtcaattact atattgatac acccgatttc aaattaaagg aacatcgctc
     8821 tgcactacgc ataagagtta aagacaatca atatgaaatg actttaaaga caccagcaaa
     8881 ggttggtcta ttagaataca actatatcgt cgacattaaa ccagagatga acttgattat
     8941 ttcaaacgac aatcttccag atgacattcg tcaaattata gttgaacagt ttggtgtaaa
     9001 ggatacaact ttatcaatac ttggcgcatt aacgacatat cgccaagaaa ctaaatataa
     9061 aggtgattta cttgttttag ataaaagtga gtatttcgac actacggatt atgagttaga
     9121 gttcgaagtt aaagactata atcaaggttt agaaaagttc caatctttat taaatgaact
     9181 aaacctcgaa catcatcaac ctttaaataa agttcaacgt ttttttaaga aaaaagaaac
     9241 actttcaaac aatataaatt aaattggcaa atatgcatat ttctttcaaa tgtttaaaat
     9301 catgttatat tagttatata ttaacttaag ttcgtaacag taaatttttg aaaatgggtg
     9361 attaaatgac aacaacacca tatgacatca ttggtaaaga agcgttatac gatatgattg
     9421 attattttta cacccttgta gaaaaggatg aacgacttaa tcacctgttt ccaggagatt
     9481 ttgcagaaac aagtcgtaaa caaaaacaat ttttaacaca gtttttgggc ggtccaaaca
     9541 tttataccga agaacacgga catcctatgc taagaaaaag acatatggcg tttacaatca
     9601 ctgaatttga aagagatgca tggttagaaa atatgcagac agcaattaat cgcgctgcgt
     9661 ttccacaagg tgttggagat tatttatttg agcgcttaag attaactgct aatcatatgg
     9721 tgaattccta aaattaattg taggtgaata aacatggctg gagaattacg aataatggaa
     9781 aataagagtc gtgaagatat taatctatca cctgtaagta aaatcgaaat ttattcattt
     9841 ttcgatccat ttagctccga ttgcttcaaa ttatcagcaa tcttatccaa attaagaatt
     9901 gaatataatc aatatatacg tatcagacat atattaaatc cttcgttaaa agtattaacg
     9961 aaatgccaag ctcaaagtac atccaacttt gataacatcg ccctagctta taaagcagct
    10021 gagttacaag gtcgtttacg tgccgaacga tttatacatt taatgcaaaa tgaaatcata
    10081 cctaaaagag atattattac agaatcaatg atttgtgact gtattcaaaa tgcaggtatt
    10141 gatttagaag tatttaaaga cgacttacaa aaaagtaaat taaccgaaag cttgaaaatt
    10201 gatttgcata ttgcaagaga aatggaaatc gaacaagccc cttctctcgt tttctttagt
    10261 gaagatgttc atgaagaagg tttaaaagtc gaaggattat acccatatca catctatact
    10321 tatataatta atgaattgat gggtaaacct atcgaaaaga atcttcctcc taaattagaa
    10381 acttatatac agcaacaaca acttgtaacg atggaagaat tacttactat ttatgaatgg
    10441 ccagaaaaac ttttaaacaa agagttaaag aagttagcta ttcaacaaaa aattgaaaaa
    10501 cttaaatatc cagatggcga tttctggaaa tctaaaatgc ctaaaatcaa atcaaaataa
    10561 ataaattaaa gacctcgtac accataaatg taacgaggtc tttttattga ctcttaattt
    10621 tgcaacatta aattattaga acgtaatgtt acgcatcgat ctaaatatat tagtgatgtt
    10681 tttcgaattt aaccagttcg aaaattatac aaattatgaa tttggtacat aatagacata
    10741 tattaatcgt taatgtataa aaagtgcata tttaatacat ttttatacaa cttcacttcc
    10801 ctcttattaa aaaagaacat aaaaaaacgt cgtgtctgaa tacacgacgc taaacaaagg
    10861 ggatgggaga aatttttcac ttcaaacaaa ggggtatgtt tgttatgtga ttaatttcat
    10921 gttcataata taacacgcta tctctcccat ttcaaccttt taatagtact taatttaaat
    10981 tgtcacaatc tgttcataat gtaagcgaat acaatagtgc taagctttca ttaatttttc
    11041 aaaagcgttc aatttttgtt caaaaacttc acaagcttgt tcaattggtt caggtgttgt
    11101 catatctaca ccagcatttt ttaatatctc aattggataa tttgagctac cttttttcaa
    11161 gaattcatta atatatctat ctactgctgg cttaccttct gttaaaattt gatgacttaa
    11221 gctttgagct gcactgtaac cagttgcgta ttgatataca taataattca tatagaagtg
    11281 tggaatacgt gaccattcct tactaatatc ttcatcagtt tctacagaat caccgaagta
    11341 taatttattt aatttggcat attcttcatt cattctagtt ggcgttaatg gttcacctgc
    11401 ttcttcaatg gcatgaattt tatgctcaaa ttctgcgaac attgtttgtc ggaataatgt
    11461 agctctgaaa cgttctaatt cttggtttaa taataataag cgtttttcat catcaagatg
    11521 tttatccata taatcactta aaagtgcttc gttacaagtt gatgctactt cagcgacaaa
    11581 aatagtgtag tcactagaat ttgacggttg gaattttcta ctgaagtaac tatgtgctga
    11641 atgtccaaat tcatgaacta atgtgtataa gtctgaaata gtattagacc agttaagtag
    11701 aataaatgga ttagttaaat gtgcacctga tgaatagcca cctgaacgtt tacctttatt
    11761 ctcatagaca tcgacccaac gattgtttaa gccttcttta actacattta aatattcttc
    11821 acccattggt tctaaagctt ttagcatcca ctctttagct tcttcataag gcatttcaaa
    11881 cttaatatct ttaattaatg gtgtatataa atcatacatt ttcaagtcat ctaaacctag
    11941 caattctttg cgcaattcag tatatctatg tagcaatggt aaatatttat gtacagtttt
    12001 tactagattg tcatatacat tttctggaat atgattatta ctcaatgctt tttctcttgc
    12061 agttttgtaa ttgtgtgtac gagcattaaa tacatttttc ttcacttcac ctgctagcgt
    12121 agcgccaagc gtattattat gagcaccata tgctttatat acatttctaa aagcactttc
    12181 tcttagtttg cgatcatctg attctaaata cttaataaat gtaccttgtg ttaacgggtg
    12241 tgcattttca tctttatcaa tcgcatcttc aaatactaaa tcagcgttgc taaacatacc
    12301 gtatacattt gatggcgttg ataacgcgtc ctgcgcttct gttaataact tttccgtttc
    12361 agcatctaaa atatgaggac gtttttcatt aatcaacttc aaatcaaatg cgaatttttg
    12421 taatttatca tatgaattta caaatgattg aattttatct tcatcaattt gtaaaatctc
    12481 tggcactaag aaactccatg ccgagctaaa tttaataatt aattgatgtg ctcttgactc
    12541 cataccagta tacttgtcgt tcgttgtatc ttggtcttgt tttagatgcg cgtatacata
    12601 tactttttct aatttagtac ctaatgtatc ttctaattct aacgcattgt ataatgtctc
    12661 agcactatca ccaatgtgtc ctttaaattg ttcttctttg cctaactcat tttcaacttc
    12721 tttaaatgca gcctcaaaag cttcatcatc tttgaaaatt gttgttaagt cccatgtata
    12781 ttcaggatat ttacgttcct gttcttctct cgataattgt tgactcatat gtagtaaacc
    12841 tcctaataaa atataattga tataattttc tcattttaga acaaaatttg cacgtcatta
    12901 cctctatctg aactaatatt taaataattg ttaataagtt tttctataat tgctgtttta
    12961 ctttctagag caacaataaa ctttttcagt ttaaaataat taagattgtc acttactaat
    13021 ttgctttttc cattctttaa ccataaatca acttgtaatt gccactcaat tggatgattt
    13081 tcaatataaa tttgctctgg aaaaatataa ccataattgt gcactacttc gtgatcagtt
    13141 aactgtaact gatacattgc acttaaagtg ggttctaaaa cagaattttg ccagcgacaa
    13201 tatttgatat attgattaat tgcgaacttt gataatttat aaacagcatg ataatcacaa
    13261 ggcgcctcat taaacaactc actaattttg gcattctttt tttcagcgac atatttgtgc
    13321 ccgcctaaat attgtatttg ttgaaacata attatttgtt ttttctctaa attgaacgta
    13381 tgaagttttc gagtgattgg attaataaac atactttgaa aatgattgaa cttcacttta
    13441 ttatgacaat aatcaacatc atttaataac caacttactt tatatcccat tttctttaaa
    13501 ccttccgttc gttgaagaat ctgtttataa ggaattggcg aatactgtag ctcaataaca
    13561 tatttattat taatcaaaat atccggaatt tgttttattt cttttaaaaa tggctctatc
    13621 tctactttac atccttgttg ctgtatcatc tgtgctaaaa tcaatttact tttataatgt
    13681 ttaatcgatt cattattaaa acatttttgt tccgctaaat gtttatgtgc aaaatgacta
    13741 attactttga gcccacgctt taaaataact tgcttgccac acaccggaca aaaatattgt
    13801 gtctttctca atgcagtagt tgctaacacg cgttcctttt cttcatttaa agctactaac
    13861 atatcttcac ctcaaaatat tacacgtaag tttaactact attacttttt cagcttttga
    13921 aacaataaaa aaaagagact ccaaagagtc tcctttcata gtgcgttgct taattaatat
    13981 cgttattcag ttgtctccgg aaaataacgt cgaacttgag ctgttacgtt atgactcata
    14041 attattttag catagtcatt taaataaact tctgttctgt ctgttggata agcaaattca
    14101 agcaattgac tgtaactatc attaatgacc tcttgatcaa catgactatc aaaatgtaca
    14161 gcataataat aagtaccatc aaccatatat aacaaatctt caaactctgt agttattgga
    14221 ttgctatgat atgcataatt aataacatct tctaaatcgt taaatttcac aataattgtt
    14281 cttgtatttt tacgtgctga agacttttga cgtttagaac cttgagcctc tttttctttt
    14341 gttcgttgct cgaataattc ttctaattga tcttcacctt ctaatgtttg agctaacaat
    14401 tcttgaactt gttcatcaaa ttgatcagtt gcatcatcat cagacatatt catcatatct
    14461 tcatttttag atttagaaat tgtgacttcg acaccttttt caaaggcatg tacttgaatc
    14521 cataatggac cttctacaac aaaatcttct tcttcgttaa tttcatccat cattgaccaa
    14581 aagaattctt cgccacgttt gcgatttgtc cataaatctt cacgactaaa tccacgggcc
    14641 tcgatatcgc tatatgttat aaacaatttt acagttgtat catctactcg ttctattctc
    14701 atatcatctc actccttaca gtcgatgaat agtatccaca ttgtattaaa gcatcaaaca
    14761 aaatacaata tatttgtttg atttctttct ctaaaatatt ttggtaactt ataataacat
    14821 attcgttgta gcaaaactat ttaaatgatt tatacagtta aagcattaaa gcactttctt
    14881 aatttaattt taccttatca tagcaaatgt ttcatccaaa acactctaat atagagctat
    14941 attaacaatt gcacatttga tttaaaagat actaagcaat tattgtaaat ttaaaaccaa
    15001 aaaagcaaca ctatagaaat agcgttgctt gacatatctt ttagtaaagt atgacacata
    15061 gttaaatggt tattagtcaa ccatacgttg tgcttcttgt aattggaacg tacgaacttt
    15121 tctaggtaag aaacgtcgaa tctcgtcctc attataacca acttgtagtc gtttattatc
    15181 taaaataatt ggacgacgta ataagccagg attatcttga atgattgaat ataagtcttg
    15241 taatggtagt gaatcaatat caacatttaa tttttggtat gttttagaac gtgtagaaat
    15301 gatttcatca gtaccgtctt cagtcatttt taatatttgc ttaatttcat caattgttaa
    15361 atgttcagaa aaaatattac gctccgtata cggaatgtca tgttcttgta accatgcttt
    15421 cgctttacgg caagatgtgc aacttggtga agtaaataat gttaccatac atctcactct
    15481 cctatttgaa tgaataaaat tcattgctta aaatttagtt atagatcaag aaaaaacatt
    15541 tttttcta
//
LOCUS       DAKSQE010000063        15404 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.063, whole genome shotgun sequence.
ACCESSION   DAKSQE010000063 DAKSQE010000000
VERSION     DAKSQE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15404
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.063"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(713..1057)
                     /locus_tag="O0V33_001672"
     CDS             complement(713..1057)
                     /locus_tag="O0V33_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HCX8976301.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            complement(1126..2250)
                     /locus_tag="O0V33_001673"
     CDS             complement(1126..2250)
                     /locus_tag="O0V33_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCX8976302.1"
                     /translation="MNALFIIIFMIVVGAIIGGVTNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIASQLNNYYTKHQNQTIASLLPNQLVTFLDQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFNEKGKLIGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARAIVTSLITNEYQTFKDKSLNKLLDTSQFNEIAENLSMYVTTYASKQ
                     ANKPVVTLMPQFVDYLESQLSSKLANLIIEQLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            complement(2429..2893)
                     /locus_tag="O0V33_001674"
     CDS             complement(2429..2893)
                     /locus_tag="O0V33_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCX8976303.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
     gene            complement(3251..3874)
                     /locus_tag="O0V33_001675"
     CDS             complement(3251..3874)
                     /locus_tag="O0V33_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCX8976304.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPDIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
     gene            complement(3896..5008)
                     /locus_tag="O0V33_001676"
     CDS             complement(3896..5008)
                     /locus_tag="O0V33_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HCX8976305.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK"
     gene            5171..5992
                     /locus_tag="O0V33_001677"
     CDS             5171..5992
                     /locus_tag="O0V33_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCX8976306.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTVDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKETVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
     gene            complement(6448..7833)
                     /gene="fumC"
                     /locus_tag="O0V33_001678"
     CDS             complement(6448..7833)
                     /gene="fumC"
                     /locus_tag="O0V33_001678"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCX8976307.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAALANFDLGKLSEAKKDAVVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHHSDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCETMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAQYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKPEDMVDPH"
     gene            complement(8029..8424)
                     /locus_tag="O0V33_001679"
     CDS             complement(8029..8424)
                     /locus_tag="O0V33_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976308.1"
                     /translation="MLGKYWIQLLIATVIISLISIKAFPLAIGALYLPIIFKVIKLQL
                     NLSKGLIDDVNAQTFIKSNQSGIVISVICCLLITGILYYTLDGFYASLTGVLGTLVAL
                     NPYTTIVSAVLYILTAIATVEATKTKYRN"
     gene            complement(9025..9177)
                     /locus_tag="O0V33_001680"
     CDS             complement(9025..9177)
                     /locus_tag="O0V33_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976309.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
     gene            complement(9202..9801)
                     /locus_tag="O0V33_001681"
     CDS             complement(9202..9801)
                     /locus_tag="O0V33_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HCX8976310.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNIEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            complement(9960..10430)
                     /gene="trmL"
                     /locus_tag="O0V33_001682"
     CDS             complement(9960..10430)
                     /gene="trmL"
                     /locus_tag="O0V33_001682"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCX8976311.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEATNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            complement(10435..11562)
                     /gene="queG"
                     /locus_tag="O0V33_001683"
     CDS             complement(10435..11562)
                     /gene="queG"
                     /locus_tag="O0V33_001683"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCX8976312.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPMIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            complement(11713..12435)
                     /locus_tag="O0V33_001684"
     CDS             complement(11713..12435)
                     /locus_tag="O0V33_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCX8976313.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            complement(12428..13885)
                     /locus_tag="O0V33_001685"
     CDS             complement(12428..13885)
                     /locus_tag="O0V33_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCX8976314.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPAAYAEQDQKWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNDYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGVVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLLVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            complement(14144..15205)
                     /locus_tag="O0V33_001686"
     CDS             complement(14144..15205)
                     /locus_tag="O0V33_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCX8976315.1"
                     /translation="MSNAKNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISLAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHELC
                     KKFIPIYSNDIYKFEVPKQ"
BASE COUNT         4908 a         2746 c         2124 g         5626 t
ORIGIN      
        1 taaagtgctt tagtcaaatc gtgatgtttt atttattaac aagtttatta tttcttcatt
       61 ttaccataat acgcttcata tcgtcgatga acatatgaaa ttgagacaca tttataacag
      121 tttaaaattt tatttgtagc gtgcgttgat ttttttcaaa aatttcagtc gaaaatgtaa
      181 tttccgtaat tgttaacacc gctagtgaac caactgatac attaaagatt tacatgatta
      241 ctctactaga tttgcttggc agtaatgacc aattagaagc taaatgtcaa aactatcatt
      301 gcacttaata cgccaaacat tttcaagccg ttcttaatga gtcattaaaa gtgaaacact
      361 tttccatcag tccgatacat gaccgaacta atagtattat gctcaaaatt ttacactcca
      421 aaatgcaaaa aggaagttag gacacgttta ctagcctaac ttccgttatc aagctcatcc
      481 gcccgttttg gcatagagcc aatattgttt gattgcccag cttcagtcgt ccgttctgag
      541 ctaaagttaa gacatgttta ataacctaac ttccgatatt gaacgcatct gcccaatttg
      601 acagggggcc tccaacttcc gttatcaaaa ctaaaaaaac tacccacaaa gtctaggact
      661 ctgtcggcag tttattcctc tatttaaagg attgaaaaat attattttaa tattagtcag
      721 cgtaaatttc gtctaatggt ttaacgataa tttggttgat ttcttggaat acttgactca
      781 ttttttgttc agcattcatt aatgcagaga tgttttcatc tttttcaatt gcttgcgctt
      841 gttcttgcgc tttttgtaaa tcttcttcag caatttcttc accttgcatt tgtttttgtt
      901 ggaagttaat ttgagtttca cggaactcgt cgaataactt tttagattct tcgttagctt
      961 ttacattagc gaatgcttct ttgattgctt tgtattcttc gctttctctt aaagcttgtt
     1021 ctaattgatt tgcataatca tataaattta ctgccatggt tatagcactc ctttgttgtg
     1081 atgtttatta aaacgttcga tattacttta ccacatctcg cttttttaga caaagattgc
     1141 aaccaaacct tggaaaaatc caataatacc acctaaaatg aaacctaatg acataatcaa
     1201 ctttagttct ttgttagcaa tttctataat taatttttca atataatcta agtcaaacgt
     1261 attaatttgt tcttcaatta atcctcgtaa atccactttt ttcataattg tagataagtg
     1321 aatcgataat tgttcaatga ttaaatttgc taatttgcta gagagttgac tttctaaata
     1381 atcaacaaat tgaggcatta acgtaaccac tggcttattc gcttgtttgc ttgcatatgt
     1441 tgttacatac attgatagat tttcagcgat ttcattaaac tgcgacgtat ctaacagttt
     1501 attcaatgat ttatctttaa aagtttgata ttcattggta attaacgatg tcacaattgc
     1561 tcttgcttta ggatgagatg ttagacgtat aagttcttgt tgaattcgat ctgcaatgct
     1621 ctcttttgtc ataaacattt gcaacatacc aattaactta cctttctcat tgaaaaaagt
     1681 atccaacata tcattaatat cttgtgtgcc ttttgcagat gataaataat ttcttgcacg
     1741 atcacataat aagtctgttg cgttatctac atgctgatct aaaaatgtta caagttgatt
     1801 tggcaataaa gatgctattg tttggttttg atgctttgta taataattat tcaattgtga
     1861 tgcaatatat tgatttccat tagtttgtaa tacttgttct aaatcaatat cgatttgaga
     1921 agtaatttgt ttaattgaca attgatcttt cgtcaacttt tgtaactgct gttgaatcat
     1981 agattctatt gcttgttgtg attgctcgct ttttaatttt tcattaatta aagtttctgt
     2041 aagcaaatgc tcttcaatca cttggccaat tttagttgca atttcttcgc gtcttttcgg
     2101 tattaaacct ggtgtaaatg ggactctaaa tttaaatata tagtatggtt taaaggggtg
     2161 aaacagcatt ctaattgcaa ttacattagt aacaccacca attatcgccc ctactacaat
     2221 cataaatatg ataataaata gtgcattcat aattttgact cctttatgac aacatagtta
     2281 ttcattatac cactattttt attttttaat tcattaatca tttcactttc tgctcaattt
     2341 attattagtt tacatacaaa aagcgatgcc tcacctctcg aaacaggtgt gttacatcgc
     2401 ttttattatt tattaaaagt atataggcct agatatgtac taattcttct ttagcatttc
     2461 tgttaaagta tgtttcagct tctctcattt tagaagtacc gaaaattggt tggttatctg
     2521 gatttaatac acgataaata ttgcttactt tgttactttg taaaatgtac ccattacgag
     2581 tctcaatgtt attccaataa atatcacttg ttggtgcatg gtttggatat gcacagtgat
     2641 ttcttgaaat tgtttgaacg atttctaatg gatcacagcc aaatgtactg tttaatactt
     2701 cagagtctct gaataatgca caaattgaaa tacaagttgt ccagtttggt aatactctct
     2761 ctttttcgat ttgtactaaa gtctttttag aaagtccaat tgtttgcgcc atagtatctt
     2821 gcgtataacc agcctctata cgaaccattt taaattttgt ttgaattaaa tctgtaaaac
     2881 tctgtctatc cattctgtta tctacctttc tgtttgggga attttatccg gacacaagaa
     2941 attgcaataa tacacatttc ttgaaacaca gattacatct taatatattt ttaataaaat
     3001 gaaaagagtc aatttcacat ttgtattaaa ttttgatcaa gtcaacaaaa gttaagttta
     3061 cttcataatg aaacaaaagt aaacgaagtt aaattttatg cattatgtct acatttgata
     3121 aaacttacac ttttattatg atatcaaatt tacaaagaaa actgtataat aaatgctatc
     3181 gctgtaaaaa aaatctaaat aaatcgatta aaaattgtcg attttttgac gaagctacat
     3241 caacgcaact ttaaatcaac ttattttcca ttgcataaat tgctgcttgt gtacgatcgc
     3301 taacttgtaa tttactaaat atatgactga catgtgtttt aattgttttt tcagatacaa
     3361 ataaagtttc tgctatctct ttatttgttt tacctttaac catttcacgt aacacttcaa
     3421 tttctctctt tgacaactta ttcgtgtagt gtggtttttg gctaactgtt tcgaatacat
     3481 cttgtgcctt aggatgtatc attttttcac cgttcataac tcgtctaata gtttcaatta
     3541 attgctgagg ctcaacgtct ttcatttcat aaccatcagc acctttattg attgctgaaa
     3601 ttacatgttc atcatcaaca taacttgtta ataccaaaac tttaatatcc ggataatgtg
     3661 ccttaatata ttccgtaatt tcaataccat tcatgccagg catcactaaa tctaatagca
     3721 caatatcagg atgctcatgc tcttttaaat attctaaaaa tgtttctcca tctgcaaagt
     3781 cttgtaaaac ttctatgttt tcaatcgtgg ataataaaaa tcgcaatcct tgtcgcacaa
     3841 tataatggtc atctactaat attactttgt tcatgggtta tctccttaaa tcaagctatt
     3901 ttataggaat tgtaaattgt atttgtgtac cctttgttgg ctgagaatga aaggtcactt
     3961 tacctcttaa taatttaact ctttgtttta tgttatttat accgtgtgat gaagctatct
     4021 gaacattatc gatctcaaat ccttgaccat aatcaatcac gtcaatatat agtatatcgt
     4081 tcatttgttt taatgtaaga tccattttat tcgtatcagc atgtttctta acattattaa
     4141 tacactcttg taatgctctg tatatgtttt cttcgatttc attagataaa tcgattaaac
     4201 cttctacatt tacatttaat tgtatatgca ttaatttact atatgctgtc aaagcatgaa
     4261 ttaagccttg ctcaagtcca actggcttaa gttgccaaat caatgcacgc atttcattaa
     4321 cggcattttg actcgtttcc tcaatcgtct tgaaagcttg tttagcaatg gattcgtttg
     4381 acatgccata cgcagcatgt gctgttagtt ttacagaaaa taacatttga tttactgaat
     4441 catgtaaatc tctagctaaa cgattacgtt cattaatttt tgctgcttct ttttcacggt
     4501 ctgttaaata aatacgtttg atggctgacc ctaattgaaa tgcgacagac tctagcaact
     4561 ctaaatcttc atcactataa atttcagtat ttggtgacgc tacatttaaa atgccgaatt
     4621 gttcttgacc cgatttaagt ggtaccgttg catgatgtgt aatattgtca ttttggctag
     4681 gaaatgcttt agaggctaag ttaatacgag aacaattgac gatattcgac gctttcatta
     4741 gcctacgttg attaaatgct ttcacacacc aacaagaccc atctttaata tagtgacagt
     4801 ggtctgctgt caaagattgt ggtaaagcca catgtgatac aagttcatgt tcacctacgc
     4861 tattgatgaa aaatatccag cctgtcgtga aattactgcc ctcaattaaa tattttaacg
     4921 caccttgggt catgctatac atttcagttt cttcatttaa aaattcggca atctctttta
     4981 ataaagctag tcgcgtcctt tgttccatca aatcgctcca attcattttt acgtgtatta
     5041 actattatac attgagttat tatattttta aatcttagac gtgaacatga taaaatggcc
     5101 ttgattactt aatagttata tttcggagaa ctgatttgtg atatgatatt aaagactata
     5161 ggaggatttt atgaaattta aaataccaga aaactttaat gacttaagtt tacgagatat
     5221 tttccaacaa cttaaggtac ctaaaaaaga tttacatcat ttaaatatgt ctaaagatat
     5281 tactattaat gataaacctg cgcgattaat ggataaagtg catactggcg acgatgtatt
     5341 tgttccaacc atcgatgaaa aaagtaatta tgttccaagt tatcgttatg cacaaattaa
     5401 atacgaagat gatgatatgg caatcgtaat gaaacctaaa ggtgttaaga cccaccctaa
     5461 tgatttaaaa gaaagcaata ctttaatgaa tcatgtgatt tacacggttg atagcgatta
     5521 tgtcgaacca attcatcgac tggaccagga aacagtagga ttattaattg ttgctaaaaa
     5581 tcctttaatg aaaaaaattc ttgatcgcat gttagaagac aatgatatta cgcggatata
     5641 caaagcaaat gttaaggcac ttttaccttt aaaaccacaa acgattgata tgccaattgg
     5701 taaagataaa ttccattcga ataaacgacg tgtgtctcct actggacagc gtgcaattac
     5761 acacatttta acttcaaaaa tgataaaaga aactgtgtgc caacttgaaa tcaagttgga
     5821 tactggacgt actcatcaaa ttcgtgtgca tttagctgaa attggtcacc cagttattgg
     5881 tgatccttta tacggtgatt caacgttaag acaattagaa cttgaaagtt acaaaataga
     5941 gtttgtgcat cccttgacta aggaagtcat ttccgtttct ttggatgact aatttgttta
     6001 gtttggcatg atatttaaat atgcaatact gcattgtaac taaatcaagt atcaacttaa
     6061 atcggataga tggaaaatta ttaatttttt cagatgttcg gtttttttat gttttttacg
     6121 accttaggat tttatatttt gaatgtattt ggatttatag catccttgga taatgacttg
     6181 tagtcttttt caactgcgtt acgtgtatct aaggacaata catgacatca taagattttt
     6241 atcacatatt gtttggtcaa tacatgtaca acaattcatc aaataaaaaa taggttctat
     6301 aataaaatgg aatccatgaa aagtttttcc tttcatggct ctatatcaaa tcggacttat
     6361 aagttcaatt tgacatagac aaaaaaataa agcttgaaca ctacatctac ctgtaatgcc
     6421 caaactttat tttatattaa tatttaatta atgaggatct accatatctt ctggtttaat
     6481 ccatgcttca aattgttctt ctgtaacata tccagtttga attgcagatt cttttaaagt
     6541 taaaccttct ttatgggctt tcttagcaat ttgagcagct ttttcataac caatatgtgg
     6601 atttaatgca gtaactaaca ttaatgattg atttaaataa ttatcaatat tctcttcgat
     6661 tggttcaatg cccactgcac aattgttatt aaatgtttcc attccgtcag ctaaaaggta
     6721 aattgattgt agtgtattat gcataataac tggtttataa acattcaatt caaagttacc
     6781 ttgtgaactt gcgaagccaa caactgtatc attacccatt acttggactg caaccattgt
     6841 taacatttca cattgtgtag gattaacttt accaggcata attgatgaac ctggttcatt
     6901 ttcagggata gaaatttctg ccaaaccagc tcgtggccct gaagccaacc atctcacatc
     6961 attagcaatt ttcattaagt ctcctgctag cgccttcaat gttccgtgta gttgtactac
     7021 ttcatcatgt gctgttaatg catggaattt attttcagaa gatacaaatg gataacccgt
     7081 attttcagaa atatactgtg ccactttatc accaaactca ggatgtgcat taatacctgt
     7141 accaacagct gtaccaccga tggcaagatt taaaatgtgc ttcttagatt cagataacat
     7201 tgtttcgcaa cggtcaagca tataacgcca gccactaatc tcttgtccta gtttgatcgg
     7261 cgttgcatct tgtaaatgtg tacgaccaat ttttataatt gaatcaaatt tatcttcttt
     7321 ttctttcaaa gtatttctta aaagttttaa tgcaggttct aattttgttt caacctcttg
     7381 atatagtgca acatgcatag cagttgggaa tgtatcattc gaactttgtg atttatttac
     7441 atcatcattt gggtggatac tttcatcact atgatgatct tttaaataca tattagcaac
     7501 ataacttact acttcgttca cattcatatt actttgtgta ccgcttcctg tttgccatac
     7561 aactagtggg aagtgttcat ctaattcacc tgataaaatt tgatcacatg cgtatacaac
     7621 ggcatctttc tttgcctcgc ttaattttcc taaatcaaaa ttagctaatg ctgctgcacg
     7681 ctttagttgt gcaaaaccat aaactacttc gattggcata cgctctttac caactgggaa
     7741 attacgttta cttctttctg tttgagcacc ccaatattta tctgcaggta cttctatttc
     7801 tccaaaagta tcatgttcaa ttcttactga cattcaattt ctccccttat cactgtttat
     7861 ttaactgtag tatatcatta aataatttaa ttgagcaatt tatgattaaa acgttttcat
     7921 aatttgaaat aaaaatacac taatcgcacg tgcccaccct ttattacagt gatacggtca
     7981 tacgattagt gtgttatcta tcattattta gttaatgttg aactaagttt aattacgata
     8041 ctttgtttta gtagcttcaa ccgtagcaat agctgtaagt atatataata cagcactaac
     8101 aattgtcgta tatggattta gagcaacaag cgtacctaat actcctgtta aactcgcata
     8161 aaacccatca agtgtataat acaatattcc tgttattaat aaacaacaaa ttacacttat
     8221 tacaatacct gattgattac ttttaatgaa tgtttgcgca ttaacatcat cgattaatcc
     8281 ttttgataaa ttgagttgta attttattac tttgaaaata ataggtaaat ataatgcccc
     8341 aattgccaat ggaaatgctt taattgatat taaacttata ataactgttg ctatcaataa
     8401 ttgaatccag tattttccta acataaatat ataaatctcc tctaatttca ttcttcaata
     8461 gcatatcata atcttggcat attaagaaac gcggtttaat gatttcatta aaaatattac
     8521 tgatagatga cttctttcaa ttatgtctgg agtaattaat tatcaattct gtttaaatgg
     8581 tgttttaaaa tttaaaattg aatttttgat atattactat gtctagtaca caaatcaata
     8641 ttctatgctc tacaaagtta tattggcagt agttgactgt agtccccaac attgaagctt
     8701 cggcatgtca gcttactatt tcatgcaagc tggcggggcc acaacacagt ggaattggat
     8761 ttccaatttc tactgacatt gcaagttggg gaacgacccc aacatagtga aatcggattt
     8821 ccaatttcta cagacattgc aagttgggga acgggtccaa catagaagct ggcggaaagt
     8881 cagcttctgt gaatataata aaaaagctag gtaacaaaat gctacctaac ttcatattca
     8941 agattatcaa tcctatttga tataggttct atactattca ttgtttacat gataaatagc
     9001 tgtatattac acaattataa tactttactg actgtcttct tcagaattct tttcttgatc
     9061 attttgatca gaaatttgtt ccatttcttt acctaattct tttaaatctt caaaatcagt
     9121 taccatactg ttttcttctt catgataatt taattttgga tctttgtctt tagacataat
     9181 caatacctca cagattttta attaagcaaa gcgtgacgta aagtaagctt taacatcttc
     9241 gggtaaacct gcagccgctt ctttatcaag aataacattt accattctat gtgcttttaa
     9301 atcggctggt tcaaagcttg ttttaccatt ttcttgatat aatttttcaa ctacatctcg
     9361 tttattagca ccagtcacta ctaagaaaat ttctcttgct tccattagtc cttgacgaat
     9421 actaacattt aacttacctt gctcatcgat agaaacaact tgtaatgtta atttcccttt
     9481 attttcttta gttttaatct tatcagcgat taattcgatt gcatcttttt cataagcaat
     9541 tggataaact tgacctgctg gtacacctaa cgcttcgaaa tatgattttt tatcgtcata
     9601 atctaaaata tttatttggc taaaatcaac agcatgtttt tcaatatttt tctttaattc
     9661 atctagtact ggcgcttgat cagtatctaa atgaaaacct gcaattgtag taggattatt
     9721 gttaaattgc tttctaataa tatcagcagc atattctgct acaagttgac tattgtcaaa
     9781 gactttaaag ttcattgcca tgatttctac actcctctta gtttgcattt tacataaaaa
     9841 cggcgatatt aataattatt ataccctaac tttcaatata tcaaaccatt taactttaac
     9901 atgcttatac tctaaatata gcacttaagc atcattttta taatgaaaat gagtaaattt
     9961 taattcaatc ctggaaaatc ttgttgacgt aacgcttcat aaattaataa cgcagcagta
    10021 tttgataaat ttaatgaacg aatatgttca ctcataggaa ttcttaacgc tgtgtcttga
    10081 tatttctctt tcacccagtc tggtaatcct gtcgtttctt ttccaaaaat gaagtaaaaa
    10141 tctttgtcat gatttgaaaa atcaaaatca ctataagtct ttttaccaaa ttttgttaat
    10201 aagtaatact cgccatttgt ggcttcaaaa aatgcttcaa tactatcatg atacgtaata
    10261 ttcacaaatt cccaataatc taaaccggct ctttttaaca ttttatcatc agttctaaat
    10321 ccaagaggtt taattaaatg taaatgtgtg tttgtacctg cacacgtacg tgcaatgtta
    10381 ccagtattag ctgggatttc tggttgatat aaaacgatat gatttgtcat attactattc
    10441 tctccttgtg tccaatcctt ttatcatttc attctgaact tctgcatcct cttgatcgta
    10501 atttgcattg ataaaatctc ttgcttcttc cccaagaatt tgaccaatag cccaataagc
    10561 agttgctcga atcatcggtc tttcatctgt tgttgcaact tttttcaatt ctggaattgc
    10621 atccacttca ttaaaatgcg ccaatgctaa aatagcattt cgttgtatcg gttttttacc
    10681 acgccaagca cctgcaaggt gaccatatgt ttgtttgaat tctttattag acatacgtag
    10741 taaaggtact aatcttggct ttaaaatttc tggttccaaa atgatgtcat cttgttcggt
    10801 attaatacct ctatttttcg ggcaaacttg ttgacacgta tcgcaaccat ataatctatt
    10861 cccaatttta taacgatatt ggtcaggcat atagcctttt gtttgcgtta aaaaactaat
    10921 gcatttctga ctatttaatt ggccatttcc aactaatgca cttgttggac aacgatcaac
    10981 acaaattgta caatcaccac agctatctaa taatggatca tcaggttcaa aaggtatact
    11041 gactaacatt tcaccgaggt atgtccatgt tcctagttta ggattgatga caaagccatt
    11101 acgaccaaca aatcctaaac ctgcacgttc tgctactgcc ctatctgata atacacccgt
    11161 atctaccata gatttgattt caacatctgg aactttagat tcaataaatg cagctaacat
    11221 atctaaccgt ttacgcataa ttgtatgata atcttgaccc cacgatgctc tagcaaataa
    11281 gcctctgcga tcacctctaa cactcttagg tgcacctttc agtttgttag gataaccaac
    11341 tgcaattgct atgattgacc ttgctgttgg taaggataat ttaggctccg ttcgtaaagc
    11401 aatatcagat tcttcaaatc ctgaggcata accatttgca tgatatgctt ctagcttttg
    11461 cttcaattca tcaaagggat cggcagtagt aaatccaata ctgtcgatac caattgtata
    11521 tgcatagtca atgatatctt gctttaactg ctttgtatcc aatcgtatgc cccccataaa
    11581 ataatactat gttattttaa cacaaccatt attcatattg aggtttcatt tcacataatt
    11641 ttaataataa aaagcttgaa tatatgatat aactttgtgg tgcgcatcat atattcaagc
    11701 tacgttaggt tgttataata ctcttgctaa gaaattttgt gttctttcat gttgcggttg
    11761 ttcaaatatt tcgactggtg tgcctgactc gacgacaacg ccatctgcca taaatatgac
    11821 tttgtcactt acatctttgg caaatcccat ttcatgtgtc acaaccacca tggtcatacc
    11881 ttctttagct aggtctttca ttacttttaa tacatcacca actacctcag gatctaatgc
    11941 tgaagttggt tcatcgaata aaataacatc tggatgcatt gctaaagctc ttgcaattgc
    12001 taccctttgt ttttgaccac ctgataattg attcggatat acatctgctt tttcttttaa
    12061 tcccacttta tctaataacg acaatgcttc cttatgtaat tcatcgttat tatctttctt
    12121 taataattta ggagctaaaa taatattatc gacaactttt ttatgtggaa ataggttgaa
    12181 gttttgaaat accataccca ttttttggcg tagtttatct acttgtgtcc ctttttccgt
    12241 taaatcattg ccttcaaaaa tcacttgacc tttagtgggt acttctaata aattcataca
    12301 tcttaacaat gtacttttac cactaccaga tggacctatt attgctacta cttccccttg
    12361 attgatttca agattgatat cttttaaaac ttcattatct ccaaaaactt tattaagatt
    12421 gtttatttta atcactggca ttcaatctcc cttcaatcat gttcataatg cgtgtaagta
    12481 caaatgttaa gacaaagtat aatgctgctg ccactagtaa tggtgtgaat gggtcaaatg
    12541 aaataccttg aaccacttgt gcattaaaca taatttcgcc aactccaatt gttgacacaa
    12601 tagatgattc tttaattaaa gtgacaaatt cattacctaa agctggtaaa atgtttttaa
    12661 ttgcttgtgg cataattaca cttttcatcg tttgtctata attcaaacct agactacgtg
    12721 cggcttccat ttggccttta tcaacagcat ttatacctgc acgaataatt tcagcaatgt
    12781 aagctgagga attaataact aatgcaattg ttccacaaac aagagctgaa atgtctagtc
    12841 ctaatgcagc cgttatacca aagaatacga taaatacttg aactaacatt ggtgttcctc
    12901 ttaatatttc gatatagata gaggcaatcc atgaaataat ttttatttta cttaatttca
    12961 ttaacgcaac gaatgcacct aaaatagaac ctaatgctac accgataagt gaaattaata
    13021 ttgtaatctt aattcctttc aagaaaaaac taccatactt agaaataaaa ccactgtcat
    13081 cattcatcgc atttgcagca ttagtcatat atttatcgat taatccttta tctttaacct
    13141 ctttaatcgt tttattaatt tgtgacaata attttggtga atcttttggt actgctatca
    13201 ctgtatcttt ttcttcttca ttaaatttca cattggaaat tcctaattta ggattttgtt
    13261 ttaaatatgc ttctgctaca ggtttttcaa ctacaacacc ttcaaccttt ccacttttca
    13321 gagctaatat aacgtctggt aaacggctta atgaagtaat agatgcattt tcaatttcgg
    13381 tttgagcgat tttttcttgt tcagttccct tttgtgcccc tactttttta ttattaaagt
    13441 ctttgatatc tttataatca ttaactttat ctttctttac aagcatgata tttttagtca
    13501 tcatatatga atctgaaaaa tcaacttgct tcttacgttc aggcgttgaa gtcattccgg
    13561 aaataataat atcaattttt ccagttttaa gagctcctaa caaactatca aatgacatat
    13621 tgacgatttt taattttaaa ttattatctt tcgcaatttt tttagctaaa tcaatatcta
    13681 cacctgcata ctcagtctta ccattaactg tatgctcaaa ttccattggt gcataatctg
    13741 cagaaagacc cactctaagt tcaccgcgtt ctttaatctt ctcccatttt tgatcttgtt
    13801 cagcatatgc tgcgggattg atatacacca tagcactgct aattaacaac cctaatacta
    13861 atataaacct aattaaacac ttcatttata cagccctccc taattaatat tcataattat
    13921 acatattaat acattttgat gcaatagtgt tttataaatg tatttttatc aatataaaga
    13981 aaagcttctt tttaaaagac tttacttaaa aaatcgtcta ttatacgcga acaattctta
    14041 aattttaaaa tcaatcaaaa aaagcgctac tatcaatatc ttattgacag taacgcctat
    14101 atttatcata tattaaaaat tattcttgtc atcaagctat aaattattgt tttggaactt
    14161 caaatttata aatatcatta gaatatatag gaatgaattt tttacacaat tcatgaacaa
    14221 taaacgccga tacaaatggt atgacaaaat aaccaagagc taataaaata atattcattg
    14281 ttggatcacc tgacattcgg ttaaatgcat ttattgggcc aactaatcct gtatatccaa
    14341 atccagcaga caatggtgtt cctttaacct gaagaacgta agcaatgatt ccagtaatta
    14401 ttccatttat agtcaatggt atcgaaataa ttaaattttt caagtaaact gggatcatca
    14461 tttttgcagc tcctatgagt aaaactgcat ttacgccaat agaattgaca cgcaatgagc
    14521 cgaataagaa agtaacacaa gcagccacta tacctaggtt tgctgctcca cttcctagcc
    14581 cgtttaaact aatcgcagtt gcaatcgcta ctaacgatat tggtgttacc attaataatg
    14641 aaaatgccag actaataagt atagacatta acaacggatt taagtctgta aaagaatgaa
    14701 ttacatttcc aattgcttga gtaatttttc gaatgtaagg taatgtgatt agaccgatac
    14761 ccccactaac gataggtact aaaactggta atataattaa ttcaaaagat ccaagcttgt
    14821 tttgtaatac catatatata agacatgcaa taataacaac caaacttgta tttataatgt
    14881 cacctatacc ttttaacata aaactattat tgctatatac aacagcacct gaaccaatca
    14941 tagctgatgt acctactata gcagcacctg cgccattaaa tttaaattga tgagcagcta
    15001 aaaccccaat aataaatgcc ataaatgatt gaattagtat cactaactga tacgttaatt
    15061 ctaaaatttc attaccactt ttaaatattt ttaatacttc acctaataaa gcattcggaa
    15121 caagtgcaat aacaacacca gcaccaatag aatttaaaat cttactgaaa aactgtttat
    15181 tatcagcatt ctttgcgtta ctcataaacg acctccaatt tgaacattaa actcatcata
    15241 ttatagaaaa ttaactttaa caatgattag ttggctaatt taatctaaat ataacaattc
    15301 acaaaaggaa taaagtaaat tggaacaatc taatcagtaa acaatgttat gttaatttta
    15361 aaatactact aattacatct tgtgactaat aatgtaataa attg
//
LOCUS       DAKSQE010000064        15091 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.064, whole genome shotgun sequence.
ACCESSION   DAKSQE010000064 DAKSQE010000000
VERSION     DAKSQE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15091
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.064"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            321..731
                     /locus_tag="O0V33_001687"
     CDS             321..731
                     /locus_tag="O0V33_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HCX8976316.1"
                     /translation="MYFTGRTYYEKNITKVIIASTVIATGLLTHSNDAKAFFSYEWKG
                     LEIAKKLADQAKKDDERATKLIDEGFKGETVNDLYIIVKKLSKGDVKKAVVKMRNGGP
                     KDFYTFDLTRPLEENRKNIPLVKNGEIDSIYWDQ"
     gene            697..780
                     /locus_tag="O0V33_001688"
     CDS             697..780
                     /locus_tag="O0V33_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976317.1"
                     /translation="MVKSTRSIGINKHTELNSCTPNVGKQC"
     gene            complement(1079..1396)
                     /locus_tag="O0V33_001689"
     CDS             complement(1079..1396)
                     /locus_tag="O0V33_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding protein"
                     /protein_id="HCX8976318.1"
                     /translation="MKKNFIGKSILSIAAISLTVSTFAGESHAQTKAEKYNEYQTNFK
                     KQVNKKVVDAQKAVNLFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLVLKYN
                     TLK"
     gene            complement(1590..1757)
                     /locus_tag="O0V33_001690"
     CDS             complement(1590..1757)
                     /locus_tag="O0V33_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976319.1"
                     /translation="MKRIVLFGKVIVDCPIWLNPVDYLVKSVLLKSIKLRFQEVRKFE
                     ILVKIMNRIHI"
     gene            complement(1887..2390)
                     /locus_tag="O0V33_001691"
     CDS             complement(1887..2390)
                     /locus_tag="O0V33_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCX8976320.1"
                     /translation="MSKWIVVSDNHTESGILYQIYEAHPDADMYLHLGDSEFEYDDTE
                     LSLFRRVKGNCDFYPEFENEAVAKCNDVRAFYTHGHLYQVNRTRYLLAEQARELGCVF
                     AFYGHTHVAKYEYINGVHAINPGSISQSRSSMEESYAEVLIDDQTLQGTVNFKNRQHE
                     TISHTTF"
     gene            complement(2383..2970)
                     /locus_tag="O0V33_001692"
     CDS             complement(2383..2970)
                     /locus_tag="O0V33_001692"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCX8976321.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELILDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DRTMAQLSKEQKGQISHRRNAINLLEAYLAGDQNV"
     gene            complement(2982..3782)
                     /gene="racE"
                     /locus_tag="O0V33_001693"
     CDS             complement(2982..3782)
                     /gene="racE"
                     /locus_tag="O0V33_001693"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCX8976322.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDTTHITNIIKEWLNLSVNVERISVND"
     gene            complement(4021..4836)
                     /gene="sdhB"
                     /locus_tag="O0V33_001694"
     CDS             complement(4021..4836)
                     /gene="sdhB"
                     /locus_tag="O0V33_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HCX8976323.1"
                     /translation="MTEQSVKNTPQHEIQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(4836..6602)
                     /gene="sdhA"
                     /locus_tag="O0V33_001695"
     CDS             complement(4836..6602)
                     /gene="sdhA"
                     /locus_tag="O0V33_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCX8976324.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(6654..7268)
                     /locus_tag="O0V33_001696"
     CDS             complement(6654..7268)
                     /locus_tag="O0V33_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HCX8976325.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(7592..9373)
                     /gene="uvrC"
                     /locus_tag="O0V33_001697"
     CDS             complement(7592..9373)
                     /gene="uvrC"
                     /locus_tag="O0V33_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCX8976326.1"
                     /translation="MEDYKKRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFVDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGSKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            9539..9634
                     /locus_tag="O0V33_001698"
     CDS             9539..9634
                     /locus_tag="O0V33_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976327.1"
                     /translation="MFDYHLTFIDLLIPPFSSRKQYDDLSFNFIY"
     gene            complement(9697..10011)
                     /gene="trxA"
                     /locus_tag="O0V33_001699"
     CDS             complement(9697..10011)
                     /gene="trxA"
                     /locus_tag="O0V33_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCX8976328.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(10184..12532)
                     /locus_tag="O0V33_001700"
     CDS             complement(10184..12532)
                     /locus_tag="O0V33_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCX8976329.1"
                     /translation="MRQKTLDVLEFDKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFQQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARCSRSIKGTKPIFKEERTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKNADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEDAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(12542..14254)
                     /gene="polX"
                     /locus_tag="O0V33_001701"
     CDS             complement(12542..14254)
                     /gene="polX"
                     /locus_tag="O0V33_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HCX8976330.1"
                     /translation="MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVK
                     EVAVGNTKVSLELAYDDETIGVDFRLIEPRAFYHTLQHFTGSKEHNIRIRQLAKARDE
                     KVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITLDDING
                     DIHMHTTFSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIK
                     ALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLA
                     NACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLSADI
                     VRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNI
                     KLKK"
     gene            complement(14327..14848)
                     /locus_tag="O0V33_001702"
     CDS             complement(14327..14848)
                     /locus_tag="O0V33_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCX8976331.1"
                     /translation="MIIDFIIIIFFVYFVIVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMIALFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            complement(14849..>15091)
                     /gene="zapA"
                     /locus_tag="O0V33_001703"
     CDS             complement(14849..>15091)
                     /gene="zapA"
                     /locus_tag="O0V33_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCX8976332.1"
                     /translation="NVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGYKAAGLDTS
                     RKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
BASE COUNT         4685 a         2852 c         2081 g         5473 t
ORIGIN      
        1 taattatcaa aaacgctata tgtataacaa tttggactac ttgtctaccc aacattgcaa
       61 ttaaatgtag agaaatataa atatcgtttt aacaataaaa atcattcaat catagttgag
      121 tgacgtttac taatattatt atactatcaa taaattcaat aatacctcaa ataaaaataa
      181 taacatcaaa ttcatcaatg ttctttttca attgcattaa accttattaa tttgcactta
      241 aatttaatta atgaagtgtt aactggtaaa tgcttgaaac cgtttgtaaa atagcagtgt
      301 actcgcatta gtaagtacaa atctatttta cagggagaac ttattatgaa aaaaatatta
      361 caaaagtaat tattgcatct acagtcatcg ctacaggtct tctaactcat tcaaatgatg
      421 ctaaagcttt ctttagttat gaatggaaag gattagaaat cgctaaaaag ttagcagatc
      481 aagctaaaaa agatgatgaa cgtgctacta aattaattga cgaaggtttt aaaggtgaaa
      541 ctgttaatga cctatacatc attgtcaaaa aattaagcaa aggcgatgtg aaaaaagctg
      601 tcgttaaaat gagaaatgga ggacctaaag atttctatac ttttgactta actcgtcctt
      661 tagaagaaaa caggaaaaat attcccttag ttaaaaatgg tgaaatcgac tcgatctatt
      721 gggatcaata aacacactga attaaatagt tgcacgccaa acgttggaaa acaatgctaa
      781 cgttttggcg tgctttttta tatgctgcaa ctcccaattt atttatcatc tttacgactg
      841 tttaataaag cacgtatgat taacacaata cctctaaatc cgcgaaaatt ctaacgattg
      901 ttaatacata tgcttggcgt tatcccgtca ttcaaaacac ctatcacata cattttcaaa
      961 ggattttcta agatttaccc caatatactc atcaagtaaa atacatacaa tgtttaaata
     1021 actttcatca gattacacat agtaaaaaag ccacactcta tagcatggct tctatcaatt
     1081 attttaaagt attatatttt aaaactagat cgatttgtct ttgtaatttt ttcttttcat
     1141 agctgtgttg gaaatgaatt aagttaacag ctctttgtgc tttacggtgt gttgcaacag
     1201 ttcttgtacg tttgaacaag tttacagctt tttgtgcatc cacaactttt ttatttactt
     1261 gttttttaaa gtttgtttga tactcgttat atttttcagc cttagtttgt gcatgagatt
     1321 caccggcaaa tgttgatacc gttaaactaa tagcagctat gcttaaaatt gatttcccaa
     1381 taaaattctt tttcataatt aataacacct atcctcaaat atttattttg tatatattta
     1441 taataataga cagaagcaaa aaactacatt aactatttat taactaaaca ttaatatttg
     1501 tgtcactaca tttctgaatt tcaagaattt tgctactcca tattttaacc atcaaattat
     1561 tgatataatt tatgccttta gaaatccttt taaatatgta tacgattcat gattttaact
     1621 aaaatttcaa atttcctgac ttcttggaat cttaatttta tactttttaa taatacactt
     1681 ttaaccaaat aatcaacagg attcaaccaa ataggacaat caacaatcac tttcccaaaa
     1741 agtacaattc ttttcataac ataaatgtgt tattcatacc agttaccttc taaaaattgt
     1801 cagcaacact tttaaacaaa cattcatgtc acgacctcct agttaaatca gtctataaac
     1861 taaaaaaagc catagctact tttaatttaa aaagtagtat gactaattgt ttcatgttgt
     1921 cggtttttaa aattcactgt accttgtaat gtttgatcat caatcaaaac ttccgcatac
     1981 gattcttcca ttgaacttct agattgagat atacttccag gattaatagc atgaacacca
     2041 ttaatatact catattttgc aacatgtgta tgtccataaa aagcaaatac acaacctaat
     2101 tcacgtgctt gctcagctaa taaatatctt gttcgattga cttgatataa atgtccatga
     2161 gtgtaaaatg ctctcacgtc attacatttt gcgaccgctt cattttcaaa ttctgggtaa
     2221 aaatcacaat tgccttttac cctacgaaat aagctaagtt ctgtatcatc atactcaaat
     2281 tctgaatcgc ctaaatgcaa atacatatca gcatctgggt gtgcctcgta aatttggtat
     2341 aaaatgccag attcagtatg attatcactc acaacaatcc atttagacat tttgatcacc
     2401 cgcaagataa gcttctaata aattaattgc atttcgtcta tggctaattt gacctttttg
     2461 ttctttagaa agttgcgcca tggttctatc taatttcggt acataaaata tcggatcata
     2521 tccgaaacca ttttcgccat atttcccatc tgcaatttca cctgaaacag tacctttaaa
     2581 tacttttgtt tccatatcag ggccactcat acttatgaca caaacaaatt gtgcacgacg
     2641 atcagttgta ttaccaagct tatttaataa tttttcaata tttgcttcat cgcttttatt
     2701 ttcaccagca taacgtgcag agtatatacc tggctcacca tttaatgcaa aaacttctag
     2761 tccactgtca tcagctatga ccgttttatt caatgctttt gcagcagctt ctgattttaa
     2821 tatagcattt tcttcaaatg ttgatcctgt ttcttccaca tcaaaatcta gtattagttc
     2881 tgaaatacct attacgtggt aatctggaaa tatcacttta aagtcattta ttttcccttg
     2941 attattcgat gcaataacaa tctctttcat taaaaatcct cctagtcatt cactgatata
     3001 cgttccacat tgacagataa atttagccat tctttgataa tgttagtaat atgtgtggta
     3061 tcacctgttg caaaaaatcg atgatctgga tgttcagtat aacttgcatg ttcattacta
     3121 aatgttagca atgcactaac ttcacgagcc gtttctaatc cagacgaaat cactgtcttt
     3181 ttaccaccaa aataatcata gataggttta tagagcaatg gatagtgggt acatcctaaa
     3241 atgacagtat cagactcact attacgccaa cgtttcagtg tttgatgaat aacaatgctt
     3301 gtaattgttg gatcactata tctcatttgt tctacaagtg gcacaaaacc tggacaggca
     3361 acgccatgta cctctacatg tggatttata cgtttaatat gtgttcgata tgcttcagat
     3421 ttaattgtgc cttccgttcc tagtactaat acattttgat ttctagtcgt cattattgct
     3481 gttctagcac ctggttcaat tacgccaatc actgggattg ataaggtctt ttgtaaatat
     3541 tctaaagcta cagcagttgc agtattacaa gcaatcacga gcatttttat atcaaattcc
     3601 attaatttac gagcgatttc aactgtatat tgttttactt gttctcctgg tcttggccca
     3661 tatggacatc gcccaatatc acctaagtaa taaatcgtct catttggcaa ctgacgcata
     3721 atttctttag ctactgtcaa acctccgaca ccagagtcta ttacacctat tggtttattc
     3781 atattcggtc atccttactt ttaatttaat ttttagttta tcataactaa gcattggatt
     3841 ttagtattat gcactgtgtt taccattttt gtcattataa tataatactt attttaaatt
     3901 agctcactat catattgtca tgtaatgtaa ctataaaaaa tcccttctaa gtttttgtgt
     3961 atctccattc aatttaattt tgaaaggaac ataacatttt aactcaaaag ggattaattt
     4021 ttattctact tcatggtctg aaccaaagaa tgatttaaac atgtggaatg ttgtttctct
     4081 gttcattgct gcaatggatg ttgttaatgg aataccttta gggcaagcat taacacagtt
     4141 ttgtgaatta ccacattgct gtaagccacc tgtacccatt aatgcattta aacgttcatc
     4201 tttagtcata gatcctgttg ggtgcaaatt aaacaaacga acttgcgaga ttgcttgcgc
     4261 accaacgaat ttattatttt cagtaacatt aggacaaact tctaaacata caccacatgt
     4321 catacattta gataattcat aagctgtttg acgttttttc tctggcatac gtggccccgg
     4381 acctaaatca tacgttccat caattgggat ccatgctttc atacgtttta agttatcgaa
     4441 cattctagaa cgatcaactt gtaagtcacg gataactggg aaagtattca ttggctctaa
     4501 acgaataggt tgttctaatt gatcaacaat cgcagaacaa gattgtcttg cacgaccatt
     4561 gataaccata gaacatgctc cacatacttc ttctaagcag ttcatatccc agacaacagg
     4621 tgttgttttt tcacctttaa tattaactgg gttacgtcta atttccatta aacatgcaat
     4681 gacgtttaaa ttttcacgat atggaatttc aaatgtttct tcataaggct tagaatcact
     4741 tgtatcttgt cgtttaataa ttaattttac tgttttttgt ttcggtttag attgtatttc
     4801 atgttgtgga gtgtttttca ctgattgttc agtcattatt ttttaccccc tttagactta
     4861 cttgtgtaat cacgtttacg aggtggtatt aaactcacat cgacgtcatc ataagtaaac
     4921 tgtggttttt caaatgcgcc ttggaatgag gccattgtcg tttttaacca ctcttcatca
     4981 ttacgctctg ggaattctgg tttataatgg gcaccgcgtg attcgttacg gttatatgca
     5041 ccaatcgtaa tcacacgtgc aagtactaac atgttccata attgacgagt aaagaatacc
     5101 gcttggttac tccaagtttg agtatcttcc atatcaatat cttcataacg tttcatcaat
     5161 tcaacaatct ttttatctgt ttctaacagt ttttcatttt cacgaacaac agttacattt
     5221 gctgtcataa tttcaccaag ttcacggtgt aatttatatg cattttctgt accgcgcata
     5281 gctaataatt tatcaaaacg ttcttgctct tcagctttac gtttttcaaa aatactttcg
     5341 tccatatcag tatatgatcg atcaatattt gaaatataat caatcgcgtt tggacctgct
     5401 actgtaccac cataaatcgc tgataacaat gaattggcac ctaagcggtt accaccatgt
     5461 tgagagaagt cacattctcc agctgcaaat aaccctttaa tatttgtcat ttgatcataa
     5521 tctacatata gaccacccat tgaatagtgg acagctggga aaatcttcat tggtactttg
     5581 cgtgggtcat caccagtgaa tttttcataa atttcaatga taccacctag ttttacatct
     5641 aactcatgtg gatctttatg tgacaaatca agatatacca tgttttcgcc attaatacct
     5701 aatttttggt taatacatac atcgaaaatt tcacgcgttg cgatatcacg aggtactaag
     5761 ttaccataat caggatattt ctcttctaag aagtaccaag gcttaccatc tttatatgtc
     5821 caaattcgtc caccttcacc acgtgctgat tcactcatta gtcgtagttt atcatcacca
     5881 ggtattgcag taggatgaat ttgaatgaac tcaccattag cataaatagc gccttgttgg
     5941 taaacaatgg aagccgctga tcctgtatta atcattgagt ttgttgtttt accgaaaata
     6001 ataccagggc cacccgttgc cataataact gcatctgaac caaatgtttc aatctcagca
     6061 gttgtcatat tttgtgcaac gatacctctt gcactatcat cgtcaccttt aactatgcca
     6121 aggaattccc atccttcata cttcgtaact aatccatcta cttcatatgc acgaacttgt
     6181 tcatccaatg catataataa ttgttgtcca gttgttgccc ctgcatatgc tgttctgtgg
     6241 tgtaatgtac caccgaaacg tctaaaatct aatagacctt catttgttct attgaacatt
     6301 acgcccatac ggtctaataa atgaataatt ttaggtgctg cctctgtcat cgctttaaca
     6361 ggtggttggt ttgcaaggaa gtcgccacca tacactgtat catcaaagtg aatccaagga
     6421 gaatcgcctt cccctttagt attgaccgca ccattaatgc caccttgggc acaaacagag
     6481 tgcgaacgct ttactggtac aactgagaac aaatctacat gtgcaccttt ttctgccgct
     6541 ttaattgttg acattaagcc cgctaggcca cctccgacaa caataagatg tttctctgcc
     6601 ataaaaattt cactccccta aattttcaat ctatatttgt taaatgcgat gtattacata
     6661 aaggcaataa ttgcagtaac accaatatac gaaataacta agaatacgat taatgaaacc
     6721 catgtaaata ctcgttgtga ttttggagat tgaagtccac cccaagtaac taagaatgac
     6781 cataagccat ttgcaaagtg gaacacaaca gcaataatac aaataatata aaatattgcc
     6841 catccaggat gttgcaatgt ttcatgcatt aaatcgtaat tcacttcttt gccgtaaaat
     6901 gctttttgta aacgtgtttg ccataaatgg ataccaataa agataaatgt taagatacca
     6961 ctcactcttt ggaagaagaa catccagttt ctaaaaatcg agtaatgtcc aacattttct
     7021 tttgctgtaa atgcaatgtg tataccaaac aaaccgtgat ataacaacgg aatgtatata
     7081 aataaaaatt ctacaataat tagaaatggt aatgattcca taaagttaga tgctttatta
     7141 aacgcttcag caccttgtgt tgcttggtga ttcactaata aatgaacgac caaaaatgca
     7201 cctattggga taatacctaa taacgagtga atacgtctta gataaaattc attttttgat
     7261 tgagccaaaa ggagtccccc ctgtgaacga atatttaatt tattgagcta tttatattaa
     7321 acgtacgctt aaccccctaa agtgatatcg tttctagcgt tgtcattatc attaagcgaa
     7381 acattttaaa gacaaataca cactgcacga tcaccaaacc gcatgtcgaa caatgtaaaa
     7441 tttggattta atatttaaga ttgcttgtga tgataaactt tctcatttag aaaacgcttc
     7501 cacgtacatt caaaaaaata actttgttaa ccatattgta acattatttc atatattttg
     7561 gggcatgaga atgattctca ctccctgtaa tttatttatg caattgttca tgtaggttct
     7621 ttgcaacgtt ttcaggaata cctatatttt taaaatcttc aagtgtagct tccttcattt
     7681 tcttgattga accgaatgaa cgcaataata atgttttacg tttgctaccg ataccatcta
     7741 tatcatcaag gattgatttc aagcctgtct tttgacgtgt ttgtctatga aatgtgattg
     7801 cgaatctgtg aacctcatct tggatacggt gcaacaaata aaatgcttgg ctatttttct
     7861 tcagtggtac aatttctgca ctagcgccat ataataattc agatgtttgg tgtttatcat
     7921 ttttctgcaa acctgcaaca gggatatcaa gacctaattc gttttgtagc acatcaataa
     7981 ccccgttcat atgtccttta ccaccatcta ctattattaa atcaggtaat ggtaatcctt
     8041 cgtttaaaac gcgagaatat cgtcgtctta ctacttctct cattgatttg taatcatctg
     8101 gacctttaac cgttttgatt ttatactttc tataattttt cttgtctggt ttaccgtcga
     8161 caaatgtaac cattgctgac actggatcca caccttgaat attagaatta tcgaatgctt
     8221 caattctaat tggtgtttga attcccattt gtgttccaag ttcttcaata gctttaattg
     8281 ttctggactc atcacgtgat attaattcaa atttattatt taaggatact ttagcgttat
     8341 gtgcagctaa gtcaaccata tcttttttgg gacctcgcgc gggttgaacg attttagtgt
     8401 ccacaacaga ttgaatcatt tctttatcca aattacgtgg tacatgaact tccttaggta
     8461 aaatatgttg gtttaagcta taaaattgtc caataaatgt ataaaattct tcttcttctg
     8521 tttgctgtaa tggaatcatc gttgtatctc gctttatcat attaccttgt cttataaaga
     8581 aaacttggat acacatccat cctttatcaa cactataacc aaagacatca cgaatcgttt
     8641 tatctgatga cataattttt tgtttgttag ttagattttg aatatgttga attaaatctc
     8701 tgtattcttt agctcgttca aaatcaagtg attcacttgc agttaacatt ctctcttcta
     8761 aactttttaa aattgttttg tcttccccat tcagaaaatc agtaatttcc ttcgtcattt
     8821 gtgcgtattt actcaaatca acgtcatata cacatggtcc taaacattgt ccaatatggt
     8881 aataaagaca taatttatct ggcatcttat cacatttgcg atatggatat attctgtcta
     8941 ataacttttt agtttcttga gcagaatatg cattcggata cggtccgaaa tatttaccag
     9001 taccttgttt tacagttctc gtcactagta gtctaggata tttttccttc gtaattttaa
     9061 taaatggata acttttatca tcctttaata atatattata tcttggttga tattgtttaa
     9121 tcagatttaa ttcaagtaaa agtgactctg tttcactaga cgtgacaata aactcaaagc
     9181 gacgtatttc accaaccagt ctcgttgttt tagcatcatg agcgcccgta aaatatgatc
     9241 gcaatcgatt tcttagcttt ttagctttgc caacatatat cacttgatcg ttacgatctt
     9301 tcattaaata gcagcctggt tccataggta cgacatttaa tttattttta attcgtttct
     9361 tatagtcttc caaaaatatc cccccttcta attttaaaat gaatgatgct acgaaagcgc
     9421 ttcaaattta tcaccgctaa ttctagttca taatcaacat cttcgagtac gacttttcat
     9481 gcacatcatg taatattgaa cattgtttta tcaaaccaac tattacatat ctatcagcat
     9541 gtttgattat catttaacgt tcatagattt attaatccca ccattctcaa gtcgtaaaca
     9601 atatgatgac ttatcattca actttatata ttaaaaacac aaacaaaaac tggagcaatt
     9661 cattaaagaa atgccccagt cgtcattggt tgtaacttat aaatgtttat ctaaaacttc
     9721 agctaagttt tcttttggtt ggaaaccaac aactttatca actggttgac cgtctttaaa
     9781 gacgattaat gttggaatac tcatcacttc atatttagct gcagttgatg gattttcatc
     9841 aacatctaat tttaaaatgt cagctttacc ttcatagtca gctgctaatt cttctaatac
     9901 cggagcgatc attttacatg gaccacacca tgttgcccaa aaatctacta attgtacacc
     9961 agattctact tttgaatcaa aatctgcatc tgttacttta acgattgcca taaatgccaa
    10021 tcctccttaa ttattattat gatgttcaaa ttatatcaga taacatgctt catgtatttc
    10081 tatttgctca atttttatgc ttatttcaaa taacatgttt gaaatgtatt cttgttgctt
    10141 tacattattt taactgcagc taattaattt atcaaattat aatttatttt agtgttgcaa
    10201 cggtaacgcc aaatccacct tcgcttggca taccacctct aaagtcacta acacttttat
    10261 gctttttcaa atgttgttgt acaccttttt gaagtgcacc tgtaccttta ccatgaatga
    10321 tataaacttg ttcgtaatta cttaaaacgg cttgatctaa atattgatct agttcaatta
    10381 aagcatcctc ataacgatag cctcgtaagt caagttcagt tttaattgtt tgacgatttt
    10441 gacgtgtaac cattttcgtt ggtttaactt tttctttttg ttttttctct aaatcttcaa
    10501 taggtaactt cattttaata attcccattt gaacaattgc ttcttcatca ttgacaattt
    10561 ctaaaacttc acccttttga ccgtaagata atacttttac ttcatcacca gcaacaattt
    10621 tatcgtattt ttgcttttgt acattttgct ttatagattt cgcttcataa tgatcatcta
    10681 atcgtttctt cttatcaatc aattcatgtt ctttaacatc tgcacctttt tgttcacgca
    10741 attgtcttag gtctttaata atatcgtcag cttcttttgt tgctgcttta attttttgat
    10801 tcgctttttc tttagcatct tcaattagag atttttcata attttggaat tgttgatact
    10861 gcttagataa atcatcatgc acttgctccg cttcttttac aagacggtcc agttctaacc
    10921 tctgtgtctc tacacgtttg taattacgct ctaatgattc aatcatttca tttatttctt
    10981 tttcatcagt accaatcatc gtcttagcct tattaataat attcaaacta agacctaact
    11041 ttttagaaat atcaaaagca tttgaacgac ccggcacacc cattaataac ttatacgttg
    11101 gactcaatgt atctacatca aattctacac tcgcattcat aacgccttct cgattataac
    11161 tatatgcttt aagttcagga tagtgcgtcg ttgccattac tagagaacca atttttctaa
    11221 catgatctaa aatgctcatt gctaatgcag caccttcact tggatctgta cctgcaccta
    11281 attcatcaaa taaaactaaa ctatgtttgt ctgcattctt taaaatttca actatattcg
    11341 tcatatgaga tgaaaaagtt gataatgatt gttctattga ttgttcatct ccgatatcgc
    11401 aatatacgtt tttaaataca ctcaactgac taccatcaag tgttggaatc aacaatcctg
    11461 attgagccat aacaataatt aaacctaatg tttttaatgt tactgtttta ccacctgtat
    11521 tcggtcctgt aataattacc gtttcaatat cttccataaa ttcgatggta ttagctacaa
    11581 cagtctcacg atttaataat ggatggtatg ctttaggtaa atatacagta cgttcctctt
    11641 taaatatcgg ctttgttcct ttaatacttc tactacatct cgcttttgcg attaaaaaat
    11701 ctaactgacc catgacttgt tctgccacaa gtagtgcatc tttgtccgca gcaacataac
    11761 cagttagttg cgttaaaatg cgttcttttt caattgcttc atcatgacgt aatcgactaa
    11821 tttgattatt catttcaaca actgatgatg gctcaatata caatgtttgt cctgaagcag
    11881 attgatcatg tacaatccca ttaaaatctt gtcgatattc agctttgaca ggtataacgt
    11941 ttctttcatt cctaactgtt acaatagcat ctgataattt tttctgattt gcttggcttt
    12001 taacaatacg gtccaaattt tgtctaatac gttgattcgt gctagaaatt ttacttctaa
    12061 tcccttgcaa ttcataactc gcattatcat ataaatcata cgtatcgcat gtttcattta
    12121 tttgttgaaa aagatcagtt aacacaggta attgattcat cttgtcatct aatattgggt
    12181 atttaacacc ttcatcttct tcaaccaatt gattataaaa tgtcttaaat tgattttgta
    12241 cttgaattaa tctttttatc aagttaagct ctgatacatt taaaacgccg ccaatatcag
    12301 cgcgatgaat gaatgctgat actttagata agccactcaa gcttggtaaa cgatgcttat
    12361 tatagatttg agcaatctca tccgtttctt ccatttgaaa aacaaccgtt tcaaaattag
    12421 tagctggcat catttgattg accttttcca agcctaagtc actaattgtt tcattggcaa
    12481 cgagtgattt tattttatca aattctaaga cgtctaatgt tttttgtctc ataaaatccc
    12541 tctatttctt aagttttata ttattttcaa taaagtcttt aaaagcttca cgcgataatg
    12601 cgttaatcac tctatctttt gttacaaatc ctttttgcgc agttgctacg ccataattca
    12661 taaaatctaa atgatttgta tgatgcgcat cagtgttaat agttaatttc acatttggat
    12721 atttacgaac gatatcagcg ctcaaatcca gtcgatgtgg attggcatta atttctaata
    12781 ctgtattcgt ttcttcagct aatgccatta attgttcaat attcggttta taaccatctc
    12841 ttctacctat aatacgccct gttggatgcg ctatatgtcg cacgtatgga ttgcgacatg
    12901 cattagctaa tcgttccata atttgttctt ctgattggtt aaagctttga tgaatagctc
    12961 caattacgta atcaagttgt gctaaaattt catcatcata atccagcgag ccatcaggta
    13021 atatatccat ttctgtaccc gaataaatat caatttcact atattcttta tctaaagctt
    13081 taatttcttc gttttgtctt aaaagtcttt ctacttgtaa gccattagca acacgtaaac
    13141 tttgtgaatg atcagtaatt accatgaatt tataaccttt tgcgatattt gcttctacca
    13201 tgtctcgaat ggaaaacgca ccatcactaa acgttgtatg catatgaata tcaccattaa
    13261 tatcatctaa tgtaatgata ttacttagat ctttatcaaa ttcgctacca tcttctctca
    13321 tagcaggtgg tataaaattc acattaaaat gttcatatat tttggcttca ctatcatatt
    13381 gaattaatgt accatcagct tgttcaattc catattcact tactttttca tcccgtgctt
    13441 tagcaagttg tcgaattctt atattatgtt cttttgaccc agtaaaatgc tgcaatgtat
    13501 gataaaaagc ccttggttca attaatcgaa aatcgacacc aatcgtttca tcatcatacg
    13561 ctaattctaa tgaaactttt gtgttcccca ctgcaacttc ttttacttta ttgggaatat
    13621 ttaataattg ctgctgcact gcttttgggt tatcggtact tattatgaaa tctaaatctt
    13681 tgctcatttc tttaaaacga cggaagcttc ctgcagatga atattgatcg atataattta
    13741 atgtatctat ataatcaatg atttcttgat taagtcttct catttgatca attggatatc
    13801 tatctttctt agcaccaagt tgtttcacag cttctaatat gttttgttcc gttttcttag
    13861 caaatccgct taattcacta acttttccat tttcacaagc aacttgaagt gaagctttat
    13921 caacaatatt caactcttta tatagcttag caattttctt gcttccaagt ccttgaattt
    13981 tcaaaagtgg aataagacct tccggaactt cttcctgtaa ttgctgtaaa tactgagatt
    14041 caccggtctc acggtaatca ttgattactt ctgcaacacc tttaccaatg ccttttaact
    14101 ccgttacatc agatatttca tctaatggtc gttcatctaa ttcaagactt tgagctgctt
    14161 ttcgatacgc tgatatttta aaagtatttt ccccttttaa ttccatataa gtagcaattt
    14221 gttctaatag tttgataaca tctttttttg tcataataac actccataaa aagaagacca
    14281 ggacgtatca ttaatatata cctttgtcct gacctcttat gataatttat aaattaatgg
    14341 tgaaagccga taaatatggg atgtgaaaaa tcaatgaatg actcacaata gatattttaa
    14401 gctgatgttg taatgcttcg ttaggatata atgccaatag atataacgta aagtgtaaga
    14461 caattatcgt cataaacaca ccaactatca ttcccattgc acgactgaaa atatgaatgt
    14521 tttgatacgc tattatttta tcaaaagtta cgataattag atataaaatg aacttacaaa
    14581 acaatgcaat cattaaaaaa gctacaatcg cttcaaatcg attttgtaga tgattaaaat
    14641 gaaacgcaaa agttgtatta aatgctgttg ttttaggata tggaataaat acaattaatc
    14701 tttctacaat agatttgtaa aattgactgg caatccacaa tgatacaatc gttgcactca
    14761 aatgtatcat agataaccaa aaacctcgtc tgaatccaac gatgacaaaa tacacaaaga
    14821 aaatgattat gataaaatca atgatcattt attgctcacg ctgctgcaat ttgtgaattt
    14881 gttgtttcaa acgtcgattt tcttcttcta gtagtacttt ttcatgcata atattcacag
    14941 cagttagtat tgcttttctt gaagtatcta aacctgctgc tttataccct aattctttta
    15001 ttttatcatc aactaaatgt gctacatatc gtatgtgctc tgggttatct tccccaacaa
    15061 ttgtaaaaag ctgatcatta attgatacat t
//
LOCUS       DAKSQE010000065        15086 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.065, whole genome shotgun sequence.
ACCESSION   DAKSQE010000065 DAKSQE010000000
VERSION     DAKSQE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15086
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.065"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(251..1087)
                     /locus_tag="O0V33_001704"
     CDS             complement(251..1087)
                     /locus_tag="O0V33_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCX8976333.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFEDENFDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            complement(1090..2076)
                     /gene="pta"
                     /locus_tag="O0V33_001705"
     CDS             complement(1090..2076)
                     /gene="pta"
                     /locus_tag="O0V33_001705"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCX8976334.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLNLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDE
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSFITAAQALQ"
     gene            2244..2996
                     /locus_tag="O0V33_001706"
     CDS             2244..2996
                     /locus_tag="O0V33_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HCX8976335.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRLVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHIINTNEFNQFFAIS"
     gene            3544..4308
                     /locus_tag="O0V33_001707"
     CDS             3544..4308
                     /locus_tag="O0V33_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCX8976336.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLASIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
     gene            4321..4773
                     /locus_tag="O0V33_001708"
     CDS             4321..4773
                     /locus_tag="O0V33_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCX8976337.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(4915..6399)
                     /locus_tag="O0V33_001709"
     CDS             complement(4915..6399)
                     /locus_tag="O0V33_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCX8976338.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLLTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVSVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(6520..6888)
                     /locus_tag="O0V33_001710"
     CDS             complement(6520..6888)
                     /locus_tag="O0V33_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCX8976339.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            complement(7021..7401)
                     /locus_tag="O0V33_001711"
     CDS             complement(7021..7401)
                     /locus_tag="O0V33_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HCX8976340.1"
                     /translation="MVNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAPT
                     SNTPHIGEQQMNHRIANHNQMPHSQITQSTHQVTAAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            complement(7402..8058)
                     /locus_tag="O0V33_001712"
     CDS             complement(7402..8058)
                     /locus_tag="O0V33_001712"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCX8976341.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANTYLESVGKSPINWCESEA
                     "
     gene            8242..9072
                     /gene="thiD"
                     /locus_tag="O0V33_001713"
     CDS             8242..9072
                     /gene="thiD"
                     /locus_tag="O0V33_001713"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCX8976342.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
     gene            9605..9772
                     /locus_tag="O0V33_001714"
     CDS             9605..9772
                     /locus_tag="O0V33_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HCX8976343.1"
                     /translation="MIIYRQYQHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
     gene            complement(9973..10242)
                     /locus_tag="O0V33_001715"
     CDS             complement(9973..10242)
                     /locus_tag="O0V33_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976344.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLEAN
                     QCQINYVKPILINENIKFIEEHEQEIIAINDDGEIKIKISLSTKK"
     gene            complement(10245..10610)
                     /locus_tag="O0V33_001716"
     CDS             complement(10245..10610)
                     /locus_tag="O0V33_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HCX8976345.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYHCFNQPYSKEVPPLMCASL
                     WSKFDLFKKYANSELILTKSVINQTQKIKVDTIYVGHLEDIECRQIRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            complement(10585..11724)
                     /locus_tag="O0V33_001717"
     CDS             complement(10585..11724)
                     /locus_tag="O0V33_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCX8976346.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYGVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGELFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFDHGLLFKDGVTVGVDSN
                     LPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKESMIASMGIGGGLGNAALFTRF"
     gene            complement(11726..13102)
                     /locus_tag="O0V33_001718"
     CDS             complement(11726..13102)
                     /locus_tag="O0V33_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCX8976347.1"
                     /translation="MDVILEQLETHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTCLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPERLLNQCRKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICVKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQHDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSGDVTLTHKNVKQFLNKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
     gene            complement(13647..15047)
                     /locus_tag="O0V33_001719"
     CDS             complement(13647..15047)
                     /locus_tag="O0V33_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCX8976348.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVESADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIALLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFGLGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVAKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     VEHPKEALWWVKERKD"
BASE COUNT         4885 a         2749 c         2321 g         5131 t
ORIGIN      
        1 tattagaaca tttcaataga aggtcgtttc tttcatagtc atacgcatta tatataccct
       61 attctcaatc tatttaatac gtaaaacatg aaatttcctt attaaattta ttatttcaat
      121 catatcatta cttttaattt aatgatgttc aatttaaata ttaggtcaat aacatattta
      181 tgctatttat ggatactttc aaaaataaca gccccaaacg ataacttgaa aggggctgtt
      241 aaatatttaa ctattgcatt tggtctatca ttttcttgtt tctttcaatc attttatcaa
      301 aatacgtatc gtatctttgc cattcttctt gagtaattgg cgtcatattt aatacaccgc
      361 caagatcttt gattgcatat aatagtaaaa acattacatc ttgaacagta atcgttttgt
      421 taagtaatgt ctctagtgag gccatacaag atggttcaat ttcagggtat ttaaatttag
      481 tcacttcacc ttttaaagca tgttcataaa atgtttgcat catcaatgca cgttctgaac
      541 cagagccttc aacacaaaga taaatttgta cagcaatacc gcctctaact cttcgttgcg
      601 atatgcctgc aaatttctta ccatcgatac ttaagtcaaa ttttcctggg caataagaat
      661 gttcaatttc catcgtatca atatcaaaat tctcatcttc gaacattttg ctaattaaga
      721 gatacatcac agtaaacgct tcatcaatcg ttgtttctgt ttgtcctttg aacatcagcg
      781 atatatttaa tacaccttga tctagaacga cacctaggcc gccagaattt ctaacaatgg
      841 cattataacc aatctcattc gttaaataat caatgccatc ttttaaaaac ggcaatcttg
      901 aatcatgaat accaagaata acagtatgtt gatgaatcca agtacgcaca acattatctg
      961 atatatcttt gcccacactt tcacaaaatg tatcatcgaa tgcgaaagat tgcataggct
     1021 ctaatccaga agaatgatcg atatatcgcc agttgacgcc attaaaatat ttactcgcta
     1081 aatccatcgt tattgtaagg cttgcgctgc tgtgatgaat gaaagattgt atacatcttc
     1141 aattgagcag ccacgtgata agtcatttac tggagaattt aaaccttgta atactggacc
     1201 aactgcatca tatccaccta aacgttgtgc aattttgtaa ccaatattac cagcttctaa
     1261 acttgggaat acaaagacat ttgcatcacc ttgtaattta gcacctggcg cttttttctc
     1321 agcaacacct ggtacaatcg cagcatcaaa ttggaattcg ccatcaatga ttgcttctaa
     1381 tttttcttct tcagcttttt gttgtgctaa tttgacagct tcttgaactt ttgtcacatc
     1441 gtctgattta gcagaccctt ttgttgaaaa gcttaacatt gcaacttttg gatccatgcc
     1501 aaagcttaat gctgattttg cactttctac tgcaatttct gcaagtcctt gtgaatcaag
     1561 ttctggattg attgcacaat caccaaagat gtactgttca tcacctttaa tcataaagaa
     1621 gatacctgat gttcttgata cacctggttt cgttttgatg atttgtaaag ctggacgcac
     1681 agtgtcgcct gttgaatgtg ctgcaccact aactaaacca tctgctttac cagcataaac
     1741 aagcattgta ccgaagtagt tcacattgtt taataattct tgtgcttgtt cttcagtcgc
     1801 tttaccttta cgtcgttcaa caaatgattg aactaattca gctttcaatt cacttgtcgc
     1861 aggattaatt aattcaatat tagaaatatc aagattaagt ttttgcgcta aagattgaac
     1921 cttagtctca tcacctaaca cgattggtgt aacataatct gttgcttgta attgtgttgc
     1981 agctgttaga acacgctcgt cctctccttc aggtaatacg attttaacgt ttttaccaga
     2041 aagtttgtct tttaatacat ttaataaatc agccataatg tcctcctgta atataaatct
     2101 tattaatcat tcacggtata attatacgcc attttatttc ataatgccac gtcaccaatt
     2161 tgtaatcata attttatcgt tacccctttg ctttatgata aaatttataa agaactgatg
     2221 atttttgaaa aggagcgata aacatgagtc aagcagccga aacattagat ggttggtata
     2281 gtctacattt attttatgca gttgattggg catcattacg tctagttcca aaggacgaac
     2341 gcgatgcact tgtcactgaa tttcaatcat ttttagaaaa tacagcaact gtaagatcat
     2401 caaaatctgg tgatcaagct atttataata taactggtca aaaagcagat ttgttattat
     2461 ggttcttacg tcctgaaatg aagtctttaa atcatattga aaatgaattt aacaaattgc
     2521 gcattgctga cttcttaatc cctacatatt catatgtatc agtcattgaa ttgagcaatt
     2581 atttagctgg taaatctgat gaagatcctt atgagaaccc tcatatcaaa gcaagattat
     2641 acccagaatt accacattct gattatattt gtttctatcc aatgaacaaa cgtcgtaatg
     2701 aaacttataa ctggtacatg ttaactatgg aagaacgcca aaaattaatg tatgaccatg
     2761 gtatgattgg tagaaaatat gctggcaaaa tcaaacaatt tattactggt tctgtaggtt
     2821 ttgatgattt cgaatggggc gtaacattgt tctcagacga cgtattacaa ttcaaaaaaa
     2881 ttgtatacga aatgcgcttt gatgaaacaa cagcacgata cggtgaattc ggtagtttct
     2941 ttgtaggaca tattattaac acaaacgaat tcaatcaatt ctttgcgatt tcttaataca
     3001 ttggtacgtt tataaattaa taaaaaattc caagcttatc ggtttaagct tggaattttt
     3061 cgtttatctt cagtatattc ccgtatacat aagacgtgat ttggtaaata gttgaaatct
     3121 gtatgtttaa acttatatat atgtgctaat gtaatatcaa taacaaagta cactttgctc
     3181 atagcaaagt gtacccgagt agtcttcctt gggagaactt taactactat cactacatat
     3241 aaacgttaac ctcaatagaa attatacagt cgctactcta tacaattttt gtaatggtta
     3301 actaatatta ttttaaccta tttgaaatat ttgaaacata tttttgtcga atttttttca
     3361 atattttttc ctttttatac ttcaagagaa ttttaactac taaaaattcc gatgattatt
     3421 attacaatag tatcaaatat tagtttttta aaatcaataa caacttatca aaaagctcat
     3481 gtggttattt tatagtgtat aaactataat gagtattaaa ttcttataaa caatggtgat
     3541 gaaatggaca taaattcaga agaatacaaa caagaggtac ttatcaaaga tgttgtcatg
     3601 cttgctgctc gcatactatt agaatctggt gcagaaggta cgcgtgtaga agataccatg
     3661 acacgtattg caaaaaaact tggttacagt gaaagtaaca gctttgttac aaacactgtc
     3721 atccagttta cgttacattc ggaatcgttt cctagaatat ttagaattac ctctcgagat
     3781 acaaacttaa taaaaatttc tcaagctaat aaaatttcgc gtcaaattac aaataatgaa
     3841 atttctttag ccgaggcaaa aacacaactt gaaaaaatat atgttgccaa gcgtgacagc
     3901 agtcttccct ttaaaggttt tgctgcagca atgattgcaa tgagtttctt atatttacaa
     3961 ggtggtagat tgattgatgt tttaactgcg atattagcag gtagtctagg atacctagtc
     4021 actgagattt tagatcgtaa gttacacgca cagtttatcc cagaattcat tggttcatta
     4081 gttattggga ttatcgccgt tattggacat acacttattc caacaggtga cttggcaagt
     4141 attatcattg cggcagtcat gcctattgtt cctggtgtat taataacaaa cgcaatacaa
     4201 gatttatttg gtgggcacat gttgatgttc acaacgaaat cattagaagc attggttact
     4261 gcgtttggca tcggtgctgg cgttggtagc gtattaattt tagtatagga gtatcagact
     4321 atgttttgga tattaaactt tatctttagc tttttagctt caatgttctt ctgcgtcatt
     4381 tttgatgcac ctagaaaatt atatttatca tgtggtttcg ttggtacgtg tggatggatg
     4441 gtttacacct tattcttcaa cggctttaat gtgcacacta tatactctag tttcttcggc
     4501 agtcttgccc taggtttatt aagtcactat atggctcgta aacaaaaaga acctgcaatc
     4561 atttttatgg tgacaggtat catcccgtta gtgccaggtg gattagctta cgatgcaacg
     4621 aaaaatttag tattattaaa tttcagtaca gcaatcaata ccatgctaga ggttacactt
     4681 attgcaggag ctatcgcatt aggtttatta ttcgccgacc aaatttccaa attaattgtt
     4741 tctggattcg tgaaatcttt taaacgatta taaatatttg ttagaaatgc ctcagcctta
     4801 actcttggca attttatata aggttcaact gcctcgtctt aaatgtcaat ttatgataca
     4861 aaaaatcgca cgatacgcct tcaaattaag aaggtatatt gtgcgattct tttattattt
     4921 agttttttct ttttcaggtt cttcaatcat atcatcaact tcattttcac ttctatttaa
     4981 aatcaagcga cttaattcat cattatccat atttttcaat ttaggatatc gaatcacaaa
     5041 gaatattaaa ccgatgatta accatccaag taatgcaata taagatggtg cagtcagtgc
     5101 tgcaggagaa cctggcacta ataacaacgc taagaaaatg aatgatacaa atgagccaat
     5161 aatagcaaac gttttgtaaa ccggtgcata cgtattactt tgtttgttat aactgaataa
     5221 tttcgcagca gacaaacatg taataaagta ggcaatggat acacctgtag atgacatatc
     5281 tacgatccaa gtcaatgccg ttcttcctag ccaaggtgca attaaagaca cacttactaa
     5341 gaatatgatt gcgacatatg gtgttttgta tttactatgt aatttactaa acattgttgg
     5401 cattatacct gaacgtccca tagaaaataa caagcgactt gaactcatca agaatccatt
     5461 taaaccagta aatataccca tcataattgc aattgctaat acacctaatc caatataacc
     5521 aaatgctgtt tgtgtaacag cacctgttaa ccacaattgt ccatttaaac tttgatgact
     5581 tgttaataac caaccagtgt ataaaatcat gacaacataa gttaatgatg ctgctaataa
     5641 actgtacacg ataagcttaa atgtcttgtt tggtgcaaag ttaaactctt ctgctgtttg
     5701 tggaatatta tcaaatccaa catatgccca tggtgccacg gatacaataa ccacaataga
     5761 cactaaccat cctttgctag gttcagctaa cggttgtaaa ttttcaagtg caaaattatt
     5821 accaaagaat gaaccaaaga acatcaataa tacgacgatt accatcgcca cacagaaata
     5881 atattgtaat gatccagata cacttgcgcc acgaatcgtt actagcatga atacaagtag
     5941 taatacggtc gcaataatga tttccgtaat ataaacgtcc cagcccgcaa tggtgtatag
     6001 tttcccatta tttaagacat ctggcaataa gaatttaact agtaaactga atgctgtcgc
     6061 atttaaagca acaacacaga cataaccaaa agttaaaaac catgatgaga agaaactcac
     6121 atatctgccg aaacttaaga aactaaaggc aaacgcgccc cctgatactg gaaatctctc
     6181 tactaatgcg ccataactaa ccgcaattaa tatcattaat aatgcaccaa taacaatacc
     6241 aattgatgct gcaatcggac ctgactgctt aatccagtct cctggtaaga tgaatgcgcc
     6301 ccatccgata catgaaccat atgcaatcgc ccatacaaac ttttcagata ggttttgttt
     6361 taaatcgcct ctatctattt gcttattctt tttttccata aataaaaact cacctcgaag
     6421 gtattctata cccaaaagat gagtttgtaa ctctttttct tgattatttt tattaaaaat
     6481 ttaatgttta gttacatagg taatctaaag ttttaaaatt taaccagcaa atttgaatgt
     6541 cgcaatgatt aacattatcc atccaatgat gaacaatacg ccaccaattg gcgtaatcgc
     6601 acctaaaact ttaatttgag ttaatactaa aatatataat gatccactaa agaaaataat
     6661 accagcaaat attaaccagc cagcccagtt aacattgatt gaagttgtac cactaattac
     6721 acctataatt aataatgcta agccatggta catttgatac gtcgttgctt tttcccatac
     6781 tgataaatag tgatcactta tttttccttg taaaccatgt gcaccaaatg cacctgttcc
     6841 gacagccatc atcgcgttta atgcacctaa aataataaat aatttcattg ctacctctcc
     6901 ttatataatt cagtattaat aaatgtttaa atccgaacac tcgaataaaa gtatatgcat
     6961 cttgattatt aacctacata cttaacaagc tattaagtaa ttatgtttca ttgttgcttt
     7021 ttaaaaatca aatatggatt caccattgcc aatgtcatca tctgtcgcta atctttgttg
     7081 ttggtttgaa ggttgtgaat atgacttatt atgatgatgt gctgaatgcg tatttacttt
     7141 accacccatc gcttcaattt cagcagctgt cacttgatgt gttgactgcg taatttgtga
     7201 atgtggcatt tgattatggt tagcaatacg atgattcatt tgttgttcac caatatgtgg
     7261 tgtatttgat gttggagcat ataaagatgt taatatatgt atggcataca tatgtttttc
     7321 aaagtcatgg tcatgctgcg cctgctctgc ttgtactaat tcttgctcaa ttcgtgctat
     7381 taaagtttct ctattcacca tctacgcctc actttcacac caattaattg gtgattttcc
     7441 tactgactct aaataggtat tcgctttgga atacggtttt gatccaaaga atcctctata
     7501 tgcagacagt ggactaggat gcactgattt tataatacaa tgtttagatg tatcgataag
     7561 ctttattttt tgctgtgcag gtttccccca caaaataaag acaacatgtt ctttataatc
     7621 agacactgct ttaataattt catcagtaaa tgtttcccaa ccaatatcac gatgagaatt
     7681 tgcttcaccc tgtcttacgg ttaaaactgt attcaataac aagacacctt ctcttgccca
     7741 atcttgtaaa tgtggtgttt gtctaacaca tccaatatca tctgctaatt ctttatacat
     7801 attacgtaaa gatggaggga attttgcgtt aggttgcact gaaaatgcta atccatgtgc
     7861 ttggttcgga ccatgatacg ggtcttgtcc taatataaca actttgatat tttcaaacgg
     7921 tgttaaatca aacgcttgat aaatattttc cctatcaggg tatacgattg cagtcgaata
     7981 ttctttttct aaaaaatcat gcatagcttt aaagtcatgt ttcgttgtta tgtcatgaaa
     8041 aatttgcgac cattccattc aatctcacct caggacatat tttaacatat cacacttatc
     8101 taaagtgaat gacattttca aaagttacaa tagtatgaat gattcatttc aacaatcatc
     8161 aaatattgaa tgcttaatat ttttcaatta ggttaaaaat gatatgatga atacaatatt
     8221 attatgtagg agtgaagctc aatggcttta aagaaagttt taacaattgc cggttctgac
     8281 acaagtgctg gcgcaggtat gcaagcagat ttgaaaacgt tccaagaatt agatacgtat
     8341 ggcatggtcg ctttaactgc catcgttact atggataaag atacatggtc acacgatgtc
     8401 acaccattac ctatggacgt atttgaaaag caacttgaaa cggctttatc aatcggacct
     8461 gatgctatta aaacaggtat gttaggtact gaggagataa tcaaacgtgc tggagaagta
     8521 tatgaggcat ctaatgcaca atattttgta gtagatcctg tcatggtttg taaaggcgaa
     8581 gatgaagtgc ttaaccctgg aaatactgag gcgatgatta aatatttact acctaaagca
     8641 acggtagtga cgccaaactt atttgaagcg ggtcaattat caggattagg taaattaaat
     8701 tcaattgaag atatgaaaaa ggctgcaaca attatctttg ataaaggcgc acagcatgtc
     8761 atcattaaag gtggcaaagc tttagatcaa gataaatcgt atgacttata ctatgatggt
     8821 caaacatttt atcaactaac tacagacatg ttccaacaaa gttataacca tggtgctggt
     8881 tgtacatttg ctgcggcaac aactgcatat ttagctaacg gtaagtcacc gaaagaagct
     8941 gtgattagcg cgaaagcttt cgtcgcttct gctattaaaa acggttggaa aatgaatgat
     9001 tttgttggtc ctgtggatca cggtgcatac aaccgtattg aacatatcga tgttgaagta
     9061 acagaggttt aatgcttcgt tcataaaaaa tagattaacc acaccttatg atttgattgt
     9121 cattgctgtg cgcctgtaaa gttagctttt tgagtctaac tttggggctc agtacacaat
     9181 aaagtcactt tcggtgtggt tattttagtt attaaatact tgtttgattt tcacagttaa
     9241 attgtgcaaa taagttttgt aagaaatcgt tatagatagt tttatttcaa catatgtata
     9301 taatattcat ttacagtgtc acgaatcagt tatcctaaaa aaacaaatcc tttcggaact
     9361 ttattatgcc cttgtttaaa cataatgttt tatcccgctc tactaattct gccatttttt
     9421 gaaattaaaa ataacttctc aacgacttta taaaaatata ttccttttaa aacatgatac
     9481 ataaatagtc ttagtaaatt atacgttcta tgtctgataa aaagttgata cttaaggttg
     9541 atgtcacaca taaaaatgtt atttataata taaccataga gtagcaaagg aggtaatata
     9601 ggttatgatt atttatcgac agtatcaaca tgaaggcgca ccagtttatg aaattataac
     9661 caaaacgttt cagcatgttt caattaaatg tgacgattca tttagtgata ctgaaatatt
     9721 caaattgctc tctttattac aagacgatat agatcatatg aaagttagtt aaattgacta
     9781 atcgttccaa taatccagaa tttattaaac attgatatag ttgttattat tattttagat
     9841 taatgcatcc ccgtgcaaag aaagaaacgt aacctatatt tatctctgtt cccaataata
     9901 ataacaatac ataactgata ttagctgtcc tttcatctcc taattaatat gcgttcatgc
     9961 agctaatatc ggttattttt ttgtgctcaa agaaatcttt attttaatct ctccatcgtc
    10021 attaattgct ataatttctt gctcgtgttc ttcaatgaat ttgatatttt catttattaa
    10081 aataggttta acatagttaa tttgacactg gttcgcctct aatgacatag ccatcaacat
    10141 catttgactc actaattgtc ctggcacaat ctcattatgt attggattat catcatttac
    10201 gagcgccaaa tattcattta tccatatctc attaaacttc atctctactt catcgcctta
    10261 ataaaagttt gtgtaaccgt tatgacatgt tgatcatttt tagttaatgt taaagccatt
    10321 gtataacgtg tgatattgcg aatctgtcgg cattcaatat cttctaaatg tcctacatat
    10381 attgtgtcta cttttatctt ttgagtttga ttaattactg attttgttaa aatcagttcg
    10441 ctatttgcat attttttaaa taaatcaaat tttgaccata atgacgcaca cattaatggt
    10501 ggtacttctt tactataagg ttggttaaaa caatgataat atgcagccac actatcctta
    10561 gaaaatgaca catttaatcg ctggttagaa tcgagtaaat aatgctgcat ttcctagacc
    10621 tcccccaatc cccatagatg caatcatact ctctttgtca aacatataaa ataatcgcgt
    10681 cactaattgg gcaccgcttg caccgtatgg atgacctgat gctaatgcac caccccatat
    10741 atttaattgc gtatttgaaa tatttaaagc ttgttggcag gcaaccacct gtgcactgaa
    10801 cgcttcgtta atttcaatga cttcaatatt ttctatcgtt aattgatttc tttttagtaa
    10861 gttggaaatg gctggtactg gaccaatgcc aggtaaatta gaatcaacac ctaccgtaac
    10921 accatcttta aataataaac catgatcgaa acctaattcg tatgccatat ctttttccat
    10981 aataagcaat agaactgcac catcattttt catacaacta ttcgcagcgg taacggtccc
    11041 acctttgatt acgggcttaa atcggccaaa gttatctttc ggaatatgtg attttagact
    11101 ttcatcagtg ttgaatagtt ctcctttaac ggttataggt aatatttcct gagaaatatt
    11161 tccattcttt acattttccg ctgtcagttg atgacttcgg taagcaaatt catcttgtaa
    11221 ttctcttgaa acaccataca tcttggctac attttcagca ccttgaatca ttgatgggtc
    11281 gctcatttca ggtgcaaatg atgcacgctc ataaaactca ggtaatgcgg tttcgtacac
    11341 agaatgcggt cgtttgattt tccaaggtgc tcgacttgta ctttcaacac cgcctgcaat
    11401 atataccttg ccagctccgg cttgaatcat gcgacatgca tattgaacac tttcaagtcc
    11461 agacccacat tgccgatcga ttgtgacgcc aggtattgaa tctttgagcc ccgcttcaag
    11521 caatgctttt cttgcaatat tgccaccatt cccaacaaca ttacctaaaa ctacatcatc
    11581 tattttagat attacctctg gatacttctc tttaaaatgt tggaataaag gtttgagcaa
    11641 ttgttctggc tctaaatgtt ttaaagtgcc accatatttc ccaaatgcag ttcgtttagc
    11701 tgcaactatg actgcttgat tcatattata attcacctct caaatacatc gacatcattt
    11761 tttctctagc aattttacca cttgcagtgt aatacatctt ttctacatga tgaatcatcg
    11821 atggaatttc atagcgtttc actttcttat ttaaaaattg ttttacattt ttatgtgtaa
    11881 gtgtcacatc accagaataa agcaatacac ctatttgacc aaaacgctca tttggaatac
    11941 cgatgataat tgcttcatca atgctcgacg attgcgttaa aaggcgttca acatgtgctg
    12001 gatatatatt ttgaccacca ataattaaca tatcatgttg tcgtcccgtt aaatataaat
    12061 actgctcttt tacatagcca ttatcattag taacaaacca ttcatcatta tttatacatt
    12121 gttcgcttac atagccacta aacatcatat tactttttac acaaatagtt cctataccat
    12181 tgtgatcgtg attcgttgtt ttcaattcca catttggaaa tagcacacct acagattcaa
    12241 ctggtgcttg ctgattcaag ttatagctga taaaactggt ttccgatgta ccaaaaaatt
    12301 caatcaaatt tatgtcattt gcttgatttt ttatcttttt aaaaatagaa gaatgcagct
    12361 tatccccact gctaaaaaat gattggattg tatgttcatt gttataaact aacaataatg
    12421 atttaatcat cgttggaaca agaaacatag caactttgta tgatgatatt ttacgacatt
    12481 gattaagtaa cctttcagga tgaaaagtgg tctgtcctat aaaagtacga ccggaactta
    12541 aagcaaacaa taacgcatat aatgttaacg agtgcgatag tggtccgggg gctgctattg
    12601 cattttcatt ttttaacatc aacatttcat taacttcaaa agaagccaac catgaatctt
    12661 catcacgata atatgctttt ggcagtccag ttgtccctga agtaaaacca atatgtaata
    12721 aattggggta gtgctgtaat tgcgttgaat caataaacat aggtgagtct atattctgca
    12781 tgagacatgt atctccaatt acatctttaa taccatattt ttcaatcaat tgacgatgta
    12841 tagtactttg ccatttacct tccatcatca taggcacttt atgtaaacga tgcaaagcta
    12901 aataacaaat aatactttga accggtgatt tcatattaat agcaacaaca gggttaagtg
    12961 aatatttctg caaagattca actgcgctag tgatgcgggc atttagttga ctatatgtaa
    13021 ttgtttcatc atctatatgt aatgctatgt cattaggctt attttgagta tgtgtttcca
    13081 actgttctaa aattacgtcc atcaatatcc ctcactcaat gtaaacttta taatacatct
    13141 tgttaacatt atataatgaa taagtatttt tatcaggtaa acctgcctat tataaaaatc
    13201 aaacttaagc cctagataaa tttgatactg tagtaccatg ttttttatta gataagacga
    13261 tactatatgt atatagagaa aagaagttat cgcaatcgca ataactgaca caaataagtc
    13321 acccaatcta tttaagtttg tgttctctat aataatattg aaggttagag tctcaatatt
    13381 aattgcgttg gacaaaatac tctaagacat atttacatcc taacatttca atagcaagag
    13441 aattttaagt attcaaagct aaacatatgc tttaagtccc ctctttcccc cattgcgata
    13501 atcattgcag tgggggttta tgtatataca aaaactttat ttcggctaat tacattccct
    13561 aatttaaaaa gcactttaaa gttagtagcg tttactagct ttaaagtgct tttatacgat
    13621 tgattattac ttatttactg aaatctctaa tctttacgtt ctttcaccca ccataatgct
    13681 tcttttgggt gttctacata ataagctcta tcttgctcgt tatctacatt tggatatgaa
    13741 cctttgagag attttcctgc tgtgctcaaa tgtaagaatg tgatcactaa aaacccaata
    13801 acactgattg ctgttaaata atacgcaggt gctaatggat caccagtttt cgcgactaac
    13861 catgttgcaa ctaatggcgt cgtaccacca aatattgaaa cagagatatt aaaggttacc
    13921 gataaagttc gatatcttat atgactgtaa aacattgttg gtaaagaccc tggcattgtc
    13981 gcttcgtatg ttgataagaa gaatcctaat ccaaatatac cgatgactat cacaacaaat
    14041 gattgtgaat gtaataacat aaatgcgatg atactaaata atgttaaccc gccagtaccg
    14101 attagaaata ctttcttttc acctatttta tccgctaact taccaaacat taatgctaat
    14161 ggaatcatta ttgccatgac acaagtaatt aatacactag ttgtcgttgc atctagttta
    14221 ataacttgtt ctaagtaagt tggtaagtat gcggttacca tatagttcgt aacattgaag
    14281 aatacaacag ctacaaaaca cacaaatata tctttgtaat aaaatctgat gatttgtaaa
    14341 aagttaatgt tgtctcgttc tggttgtgtt gcaacatcat tttcgaaaac tggtgattct
    14401 tcaagcttac gacgtaaata taagccgaat aatcctagga ataaaccaag taagaatggg
    14461 attctccaac caaatgatgc catttgttca tcagttaaga agaaagtgag taaggcaatc
    14521 ataattgacg cagctatata acctgataat gtccctattt ctaatccact acctaatgag
    14581 ttacgacgct tatctggaga tgattcggct acatatgtca ttgcccccgc atactctcca
    14641 cctgttgaaa atccttgtag tactcgtgca agtaataata gtattggtgc ccaaagtcca
    14701 atttgatcat aacttggtaa taatccaatg gttaatgttg aaaatgccat taaaataatt
    14761 gttgatgtta atacaacttt acgtccatat ttgtcaccaa taataccaaa tacaacacca
    14821 ccaattggtc ttaataaaaa cgcaatggct aatgctgcga aagtcaacat ttgtcgaatg
    14881 tctgcactct ctactggaga gaagaagttc gctccaatgt acgcagttgt atatgcatag
    14941 acaccaaaat cgaaccattc cattgcatta ccgataccgg ttgcaacaac agtcttttta
    15001 gctttctttg catccaccat gttaatattc tctttattaa aatccatgaa ttcatacact
    15061 ccctcttgta tgtttcataa ttatac
//
LOCUS       DAKSQE010000066        14663 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.066, whole genome shotgun sequence.
ACCESSION   DAKSQE010000066 DAKSQE010000000
VERSION     DAKSQE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14663
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.066"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            147..1148
                     /gene="argF"
                     /locus_tag="O0V33_001720"
     CDS             147..1148
                     /gene="argF"
                     /locus_tag="O0V33_001720"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCX8976349.1"
                     /translation="MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLK
                     NKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYD
                     GIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
                     VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDI
                     DQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEMMDKTGNPNVIFEHCLPS
                     FHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            1171..2103
                     /gene="arcC"
                     /locus_tag="O0V33_001721"
     CDS             1171..2103
                     /gene="arcC"
                     /locus_tag="O0V33_001721"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCX8976350.1"
                     /translation="MAKIVVALGGNALGKSPQEQLKLVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFTFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
     gene            2274..3830
                     /locus_tag="O0V33_001722"
     CDS             2274..3830
                     /locus_tag="O0V33_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCX8976351.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKKDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     VLITQVLIYS"
     gene            4137..4364
                     /locus_tag="O0V33_001723"
     CDS             4137..4364
                     /locus_tag="O0V33_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976352.1"
                     /translation="MTHLTKVLDTLTGICVVLLFSKYFVTYANMVFDWNLRWYLLGNI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
     gene            complement(4680..5627)
                     /locus_tag="O0V33_001724"
     CDS             complement(4680..5627)
                     /locus_tag="O0V33_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCX8976353.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAICGIFAFLIWI
                     HLLCTMLKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINNLITPIW
                     ILCLVGIMMHMIIFSIKYLKDFSLENVYPSWTVLFIGIAIAGLTAPVSGYFFIGQLTV
                     IYGFVATCIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     ILLLLAQIFYFYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLMFPVIWKG
                     LLFIEVLLATVIVLRVFIGYLHFFLKKENQDKFLRNVSQ"
     gene            5874..6071
                     /locus_tag="O0V33_001725"
     CDS             5874..6071
                     /locus_tag="O0V33_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCX8976354.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKNERSLDNMSL
                     KNAELLYKFANGIFSDENYEE"
     gene            complement(6533..6606)
                     /locus_tag="O0V33_001726"
     tRNA            complement(6533..6606)
                     /locus_tag="O0V33_001726"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6570..6572),aa:Arg,seq:tct)
     gene            6970..7104
                     /locus_tag="O0V33_001727"
     CDS             6970..7104
                     /locus_tag="O0V33_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCX8976355.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            7228..7923
                     /locus_tag="O0V33_001728"
     CDS             7228..7923
                     /locus_tag="O0V33_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCX8976356.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFYYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHNLYIVTNGVTETQKRRLNQTPLHKYIKKIFISEETGYQKPN
                     PEFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRGFDHNPGIIPDY
                     EINSWKQLNEIVR"
     gene            complement(8031..8471)
                     /locus_tag="O0V33_001729"
     CDS             complement(8031..8471)
                     /locus_tag="O0V33_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCX8976357.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
     gene            8666..10279
                     /gene="bshC"
                     /locus_tag="O0V33_001730"
     CDS             8666..10279
                     /gene="bshC"
                     /locus_tag="O0V33_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HCX8976358.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQISFEKRMS
                     KENNGREAALANVIREYMNDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQKRTQNQGLKAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHFRLNKTDKKYIKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFIGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASDQFIEKVEGMIEQQRRLYQDLLDEVAGNQNNI
                     NLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQ
                     ILNDFGTDVFKPSTYPPLSYTFDHIIIKP"
     gene            10423..10854
                     /gene="mraZ"
                     /locus_tag="O0V33_001731"
     CDS             10423..10854
                     /gene="mraZ"
                     /locus_tag="O0V33_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCX8976359.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            10870..11805
                     /gene="rsmH"
                     /locus_tag="O0V33_001732"
     CDS             10870..11805
                     /gene="rsmH"
                     /locus_tag="O0V33_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCX8976360.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            11819..12220
                     /gene="ftsL"
                     /locus_tag="O0V33_001733"
     CDS             11819..12220
                     /gene="ftsL"
                     /locus_tag="O0V33_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCX8976361.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            12201..14435
                     /locus_tag="O0V33_001734"
     CDS             12201..14435
                     /locus_tag="O0V33_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCX8976362.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVIDMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
BASE COUNT         5347 a         2004 c         2752 g         4560 t
ORIGIN      
        1 ttaattattt cctactactt aaatttaata ttaataaaat gttcatttaa ttattgataa
       61 aatattacaa attttaatag taggttgtgt ttattttgta tgcgcttaca atttaggtgt
      121 aactaaaata aaaggagttg ttattaatga aaaatttacg aaacagaagt tttttaactt
      181 tattagactt ttcacgacaa gaggtagaat tcttattaac actctccgag gatttaaaac
      241 gtgctaaata tattggcact gaaaagccta tgttaaaaaa taaaaatatt gcactgttat
      301 ttgaaaaaga ttctacaaga acgcgatgtg catttgaagt tgcagcgcat gatcaaggtg
      361 ctaatgtaac ttatttaggc ccaactggat cacaaatggg taaaaaagaa acaactaaag
      421 atactgcacg tgtgcttggt ggaatgtatg atggcattga ataccgtggt ttttcacaaa
      481 gaacagtaga aactttagct gaatattcag gcgtaccagt gtggaatggt ttaactgatg
      541 aagatcatcc tactcaagtt cttgctgatt tcttaacagc aaaagaagtt ttgaaaaaag
      601 attatgcaga tattaacttt acatatgttg gagatggacg taataacgtt gcaaatgcat
      661 taatgcaagg tgctgccatt atgggtatga acttccattt agtttgtcca aaagaattaa
      721 atccaacaga tgaattatta aatcgctgta aaaatattgc cgctgaaaat ggtggcaaca
      781 tattaatcac agatgatatt gaccaaggtg taaagggttc ggatgtaatt tacactgacg
      841 tttgggtatc aatgggtgaa cctgatgaag tatggaaaga acgacttgaa ttattgaaac
      901 cttatcaagt aaataaagaa atgatggata aaactggtaa tccaaatgtt atttttgagc
      961 attgcttacc atctttccac aatgctgata ccaaaattgg acaacaaatt tttgaaaaat
     1021 atggtattcg agaaatggaa gttacagatg aagtattcga aagtaaagct tcagttgtat
     1081 tccaagaagc tgagaacaga atgcatacaa tcaaagcagt catggttgct acattgggtg
     1141 aattttaaat gatataagga agtgaatatg atggcgaaaa tagtagtagc attaggtggg
     1201 aatgctttag gaaaatcacc tcaagaacaa ctcaagcttg ttaaaaatac tgcgaaatca
     1261 ttagtaggat taataacaaa aggacatgag attgttatta gtcatggtaa tgggccacag
     1321 gttggaagca ttaatttggg acttaactat gctgcagaac acaaccaagg tccggcattt
     1381 ccatttgctg aatgtggcgc aatgagtcaa gcttacatcg gctatcaatt acaagaaagc
     1441 ttacaaaacg aattgcattc tattggaatg gataaacaag tggtaacact agtgacacaa
     1501 gttgaagttg acgaaaatga tccggcattt aacaatcctt caaaaccaat tgggttattt
     1561 tacaacaaag aagaagctga acaaattcaa aaagaaaaag gatttacatt tgttgaagat
     1621 gctggaagag ggtatagacg tgttgttcct tcaccacaac ccatctctat tattgaatta
     1681 gagagtatta aaacacttat taaaaatgat acactcgtta ttgctgctgg tggtggaggt
     1741 ataccagtaa ttagagagca acatgatggt tttaaaggta ttgatgcagt tatagacaaa
     1801 gataaaacaa gtgcgttgtt gggtgctaat attcaatgcg atcaattgat tattttaact
     1861 gcaattgatt atgtatatat taattttaac actgaaaacc aacagccttt gaaaacaaca
     1921 aatgttgatg aattaaaacg atatatagac gaaaatcaat ttgcaaaagg aagtatgtta
     1981 ccaaaaattg aagcagccat atcatttatt gaaaacaatc caaaaggaag tgtgcttata
     2041 acatcattaa atgaattaga tgctgcctta gagggtaaag taggtactgt gattaaaaag
     2101 taattgaatt gaaacgcttt tcaattacta tatgtcaaat gcatgatttt tattatttat
     2161 gtgcacccct gaaaataatg cctctatttt gatgcggggt gcactttctt aatttatatt
     2221 tataaaatct ttaaggtaga aatgctaggt taaatgtcga aggagatgaa accgtggaaa
     2281 atacaattaa tgaaagtgaa aagaaaaaac gatttaaatt aaaaatgcca ggtgcattta
     2341 tgattttatt cattttaacg gttgttgcag ttatagcaac atgggttatt cctgctggtg
     2401 catattctaa actttcttac gaaccttcat cccaagaact aaagatagtt aaccctcata
     2461 accaagtgaa aaaggttcca ggtacgcaac aggaactaga caaaatgggg gttaaaatta
     2521 agattgaaca atttaaatca ggtgcaatta ataagccggt atcaattccg aatacttatg
     2581 aaagattaaa gcaacatcca gctggaccag aacaaataac aagtagcatg gttgaaggta
     2641 cgatagaagc ggttgatatc atggtattca ttcttgtatt agggggactt attggcgtag
     2701 ttcaagccag tggttctttt gaatcgggat tgctagcttt aacaaagaaa actaaagggc
     2761 atgaatttat gctaattgtg tttgtatcaa tactaatgat tatcggcggg acgttatgtg
     2821 gtattgaaga agaagctgtc gcattctatc cgattttagt ccctatattt atagcgttag
     2881 gatacgattc tatcgtttct gttggtgcca tattccttgc cagctctgtc ggtagtacct
     2941 tttcaactat taacccgttc tcggttgtaa ttgcctctaa tgcagctggt acaactttca
     3001 cggatggttt gtattggaga ataggtgctt gtattgtcgg tgcgattttt gttattagtt
     3061 atttatattg gtactgtaaa aaaattaaaa aagatcctaa agcgtcatat tcttatgaag
     3121 acaaagatgc ttttgaacag caatggtctg tgttaaaaga tgacgatagt gcccatttta
     3181 ctttgcgtaa gaagataatc cttacattat ttgtactacc atttccaatt atggtatggg
     3241 gagttatgac gcaaggttgg tggttcccag ttatggcttc agcattttta atatttacaa
     3301 ttataataat gtttattgct gggacaggta aatctggatt gggagaaaaa gggactgtag
     3361 atgcatttgt caatggtgca tcaagtttag taggtgtatc tttaattatt ggattagctc
     3421 gaggtattaa tttagtgttg aatgaaggta tgatttcaga tacaatctta cacttttcat
     3481 catctttagt tcaacatatg agtggaccat tatttatcat cgtattacta tttattttct
     3541 tctgtttagg ttttatcgtg ccatcatctt ctggattagc ggtattatca atgcctatct
     3601 ttgcaccact agctgataca gtaggtatac caaggttcgt catcgttacg acatatcaat
     3661 ttggtcaata tgctatgtta ttcttagcgc cgactggact tgttatggcc acactacaaa
     3721 tgttaaacat gcgatattca cattggttcc gatttgtatg gccggtagtt gcttttgtat
     3781 tgattttcgg tggcggagta ctaattacgc aagtactaat ttattcataa tttgaaatgc
     3841 tatattataa aaaatactaa ttgggttata tgcatctcgt aggtttgtag aaatactaaa
     3901 ctaagcgagg tgcattatta ttttgattta gaaaataatg gcggtaatga taacattagt
     3961 aagtaattga tacaatgttc tatttaataa tgatattttt aaaatatgtt tttaatgtaa
     4021 tgttagatct atggtatatt atatttatcg tggtaaatat atatttgctg taaacagttt
     4081 ttgtaacaca tgatacattt ggttgtgaaa atagcataaa taaaggatgg ctataaatga
     4141 cacatttgac aaaggtttta gatacactaa ctggaatatg cgtagtatta ttatttagta
     4201 aatattttgt gacgtatgca aatatggtat ttgattggaa tttaagatgg tatttgctag
     4261 gaaacatacc acatttgcca attatattat ttattctgat gtttattttc ggagtacctt
     4321 ctgaaatgat aaaagatagg cagaggaaaa ataacggcgt ttaatttatc gatgttaaga
     4381 ggtgataaaa cttgtcaact attacaaaaa ctaaaataaa aatctttatt aattatttta
     4441 ttggtatatt agccttggcg tttgctgctt atatattatt taaataaaga atagagtgaa
     4501 acgtgtttat gaactagacg ttctagtata gttactacag ctttagtcag aaggtatcat
     4561 tgttgttcat aaataagatg atattaaatc aaagaggcat tgatatacac taaaaagagg
     4621 caagattacc tgcccctttt ttagttatta aatatgcgtg taaattcttg gtaatagtgt
     4681 tactgagaca cattacgaag aaatttatct tggttttcct tttttaaaaa gaagtgaaga
     4741 tatcctataa agactctaag tactattaca gtggctaata acacttcgat aaacaaaagg
     4801 cccttccaaa tgactggaaa cataagcaca ggcaaactgt tctttaaagc agttgcagag
     4861 attactaaag ggaatgtgaa agctgaaaat actggcgaaa aaggttcttt tagtaattta
     4921 ggcaattgta taatgatgta aaaataaaat atttgagcta atagtaaaag tataattacg
     4981 ataaaagcat tcgctttagg aaaggctata acatatgctg cagcgactaa agaaaatggt
     5041 gcacaaattg tcgatgtgtt cggtttgatt gacgtctgca atggaaatgc ttttaatcgc
     5101 ttgaaaacta taggtaagac aatacaagta gctacaaagc catatattac tgttaattga
     5161 cctatgaaaa aatatccgct gacgggtgcc gtcaatcctg cgatagcaat accaataaaa
     5221 agtacagtcc atgaaggata aacattctca agtgaaaaat cttttaaata tttaattgaa
     5281 aaaataatca tatgcatcat aattcccaca aggcataaaa tccaaatagg cgttattaaa
     5341 ttattgataa atgttatatt actaaaaaat gtatttaaat aagtagtacc taaaaagcca
     5401 gacatgaaaa atgttgtgaa cacagatgaa actagagggc tgtttaattg ttctttcaca
     5461 ttattaaaat atttgagcat agtacataaa aggtgaatcc aaatcaagaa agcaaagatt
     5521 ccgcaaatag cgtttaaagt aagagataag tcttttaata gattacccag gcctaataat
     5581 cctaagacta gtcctgacgt tactagaggt gctttttgaa gtctcatgat ttacaacctt
     5641 tcttatgtga ttttcttcac taattatatc atgattgcta tggactatta ataagaagag
     5701 agtgtactat tacgttttta gagtgtgtat attggattga tagacacata attgacattt
     5761 aaatctcaaa attaatgata tttgaggtat gctttgctac ctaaatatgg aatgtgggaa
     5821 aagtatttat ttaatttaaa tgatgattga agatttacaa gggagtgtac aaaatgagaa
     5881 atcaaattca aaaactatta gacagtgatt tgagcagttt acatatatca aaacaaacag
     5941 gagtgccaca aagcacaata cacagaatga gaaaaaatga aagatcatta gacaatatgt
     6001 cattgaaaaa cgctgaacta ctttataaat ttgccaatgg tatatttagt gatgaaaatt
     6061 atgaagaata attagtgagt taaataatta gaaaaccacg tctcgatgga cgtggttatt
     6121 tttcagatgt atataattat aattatagga tttcgggtcc ctgaaaagtc cctaaaattt
     6181 tgaatagtat caaaatctat aggaataata taataataaa aatctctacg catgcaaatg
     6241 gtgagtgtta aaaatcttgg tgtagttttg gtgtagttca ggtacagtat agaggaaatt
     6301 tgagcgcaaa attacttaat taaaaatgtg taaacaagtg tcagacgcgt gtcaattgtg
     6361 tgtcaaaaaa ttctataacg aatatgcatg cttgttttta tcactgtatt ttctgggctt
     6421 gtgcggggac gttttggtga cgcatcatac tatattactg aaattcaaaa caaaaagagc
     6481 cccgtaatca cggaactctt ttgtttggta atgcgtataa aaatactata aacgtcctgg
     6541 gagggattcg aacccccgac cgatggctta gaaggccatt gctctatcca gctgagctac
     6601 caggacacgt ttaacaacac aagaattatt atatctaaat gaacttaaat tagcaatacc
     6661 ttgtaaataa aaaatgttta tatttttcac tattatagag ctatttatct aaaaaggttc
     6721 aataagactt aaatacgaat tcaggcaact taattgtgtt aaatacagtt ttgaatgcct
     6781 aactgtatat cttttctctt taaagtacag ttaagtacat tataagatgt tgtgcggata
     6841 aacaaactaa ttgcatcaaa tttattttaa aataacaaca acaaaacgtt gagcgaataa
     6901 catttcggtg atttaaaagc tacgcacgtt tttgttatct tcaaatttaa attttaagga
     6961 gtgttttcaa tggaaggttt atttaacgca attaaagata ccgtaactgc agcaattaat
     7021 aatgacggcg caaaattagg cacaagcatt gtgagcatcg ttgaaaatgg cgtaggttta
     7081 ctaggtaaat tattcggatt ctaatataat aactaatatt ctttaaaata aactgggtga
     7141 gcatacttta atgttatgca ctcagtttat tttatttgca gaaatttgag cctctgttaa
     7201 gatttagata catagacaat ataggagatg gggaaattgg gatataaaaa tattttgata
     7261 gactttgatg atacaattgt tgatttttat gatgcagaag aatgggcatt ttactatatg
     7321 gcgaatgttt ttaatcataa agcaacaaag gatgattttt taacatttaa aaaaatcaat
     7381 caccaacatt gggaagcttt tcaacaaaat aaattaacga agtctgaagt attatcagaa
     7441 cgatttgtaa attacttcaa acatcatcaa atggaagttg atgggcatcg tgcagatgtg
     7501 ttatttagaa atggattagc agaagctaaa gttaaatact ttgatcaaac attagaaaca
     7561 attgtcgagt tatcgaaaag acataatcta tatattgtta ctaatggtgt aacagaaacg
     7621 caaaagagaa ggttaaatca gacgccattg cataaatata ttaaaaagat atttatatct
     7681 gaggaaacag gatatcaaaa acctaatccg gaatttttta attatgtttt taatgatatt
     7741 ggtgaggatg aaagacagca ctcgattata gttggagatt ctttaacatc tgacattcta
     7801 ggtggaatca atgcgggtat agctacttgc tggtttaatt ttagaggatt tgatcataat
     7861 ccaggaatta tacctgatta tgaaattaat tcatggaaac aactaaatga aattgtacgt
     7921 taagacaaat aatttgaaaa tgatttactt atattagcta agaaagtaat tgtaaaaaag
     7981 acactacgaa aagatgcatt ttcattctcg tagtgtcgaa tattattatc ttatggtttc
     8041 cacaataaga catcatgtcc ctctatattt ttagcttcca catctgtaaa tccatgatgt
     8101 aaaaagaaat ccttagaatc atttctgcca atggctttaa ttggcatatt gaaactcttt
     8161 gcaaaatcaa tcaattctga agcgtaccct ctgttttgat attttggtaa tacttctaac
     8221 ttccataata atatataatc ttcaaaatct gggaagtaga tttcttcgac atcacctttt
     8281 ttaagtaatg ccattctagc tcctaattga tctccgacaa atatgccata aaatggtgaa
     8341 tctgaacttg cgtcaatcat ttgaccgttt aactcattga ccatgtataa gtctttgttg
     8401 ccaaacgctc taaagttttc gaataattcg tcagttttgt aattaatttc aagacgtttg
     8461 atttcactca tatttatcta ccccttattt gtttactact attattatac attaaaccac
     8521 ttgtgttttt cattgatttg aaaatgtaaa acaatgaata ttataaattt tccatgatta
     8581 aattgatttt caagccgtaa aattgtcaat caccggtata gactttatac tatagtttgt
     8641 aatcgcgtac ttaaggagaa aaaacatgga ctgtaaagta gttagtttaa atgaaaaaga
     8701 tcagtttata ccaaaaataa agagcagtga tcctgtaata acaggattat ttcaatatga
     8761 tgcagctcaa caaattagtt ttgaaaaaag aatgtctaaa gaaaataatg gaagagaagc
     8821 ggcattagcg aatgttattc gtgaatatat gaatgattta aagctttcta gtgaacaaga
     8881 attaaacata caacatttag ctaatggttc aaaagttgtg attggtggac aacaagcagg
     8941 gcttttcggg ggaccattgt atacattcca taaaatattt tcaatcatta ccttatctaa
     9001 ggaattaacg gatacacata agcaacaagt agtaccagtt ttttggattg caggagaaga
     9061 tcatgatttc gatgaagtga atcatacatt tgtttataac gaaaatcatg ggtcactgca
     9121 taaggttaaa tatcatacaa tggagatgcc agagacgact gtctctagat attatcctga
     9181 taaggctgag ttgaaacaaa ctttaaaaac gatgttcatt catatgaaag aaactgttca
     9241 tacacaaggt ctacttgaga tttgtgacag aattattgac caatatgact cgtggactga
     9301 tatgtttaaa gcactactgc atgaaacatt taaagcatat ggcgttctat ttatagatgc
     9361 gcagtttgag ccgttaagaa aaatggaagc gcctatgttt aaaaagattt tgaaaaaaca
     9421 tcagttgctt gatgatgctt ttagagcaac acaaaaacgt actcaaaatc aaggcttgaa
     9481 ggcgatgata caaacagata caaatgttca tttattctta catgatgaaa atatgcgtca
     9541 attagtttcg tatgatggta agcattttag attaaataaa acagataaga aatatataaa
     9601 ggaagaaatt attaatattg cggaaaatca acctgaatta ttttctaata atgtagtgac
     9661 aagaccatta atggaagaat ggctatttaa cacggtggca tttattggag ggccgagtga
     9721 aattaagtac tgggctgaac taaaagatgt atttgaacta tttgatgttg aaatgcctat
     9781 cgtgatgcca aggcttagaa ttacttattt aaatgaccgt attgaaaaat tactttcgaa
     9841 atacaatatt ccattagaaa aagtgttagt cgatggtgtt gaaggagaaa gaagtaagtt
     9901 tattagagaa caagcatcag atcaatttat tgaaaaggta gaaggtatga tagaacaaca
     9961 gcgtcgtcta taccaagact tattagatga agtggcgggg aatcaaaata atattaacct
    10021 tgtgaataaa aataatgaaa ttcatataca acagtatgat tatttgttaa aacgttatct
    10081 tttaaacatt gaaagagaaa acgacatcag tatgaagcaa tttagagaaa ttcaagaaac
    10141 actccatcca atgggaggat tacaagaaag aatatggaat ccacttcaaa ttttgaatga
    10201 ttttgggaca gatgtgttca agccctccac ctatccacca ctttcttaca cttttgatca
    10261 tattattata aaaccttaat ataacaaggg tttagcccga tttatcataa tgataaatcg
    10321 ggcatttttt tgttttttaa aataaatttc acaaattttg tataaatagt ggtggatagt
    10381 ggggagatgt ggtaaattat atataaggtg aggtgataaa aaatgttcat gggagaatac
    10441 gatcatcaat tagatacaaa aggacgtatg attataccgt ccaagtttcg ttatgactta
    10501 aatgagcgtt ttattatcac aagaggcctt gataaatgtt tattcggtta cactctagac
    10561 gaatggcaac agattgaaga gaaaatgaaa accttaccta tgacaaaaaa agacgcacgt
    10621 aagtttatgc gtatgttctt ctctggtgct gttgaagtag aacttgataa gcaagggcgt
    10681 attaacatcc ctcaaaactt gaggaaatac gctaatttaa ctaaagaatg tacagtaatc
    10741 ggtgtttcaa atcgtattga gatttgggat agagaaactt ggaatgattt ctatgaagaa
    10801 tctgaagaaa gtttcgaaga tattgctgaa gatttaatag attttgattt ttaaaatgga
    10861 ggaattgaag tgtttcatca tatcagcgtt atgttaaacg aaaccattga ttatttaaat
    10921 gtaaaagaaa atggtgtgta cattgactgt acgctaggtg gagcgggaca tgccctttat
    10981 ttactaaatc aattaaatga cgacggaaga ttaatagcaa tcgatcaaga ccaaactgca
    11041 attgataatg ctaaagaggt attaaaggat catttgcata aggtgacttt tgttcatagc
    11101 aacttccgtg aattaactca aatattaaaa gacttaaaca ttgaaaaagt agatggaatc
    11161 tattacgact tgggtgtttc aagcccacaa ctcgacattc cagaacgagg attcagttat
    11221 caccatgacg caacattaga catgcgtatg gaccaaacac aagaactaac agcatatgaa
    11281 attgttaaca attggtcata tgaagcgtta gtgaagattt tttatcgcta tggcgaggag
    11341 aaattttcaa aacagatagc tcgaagaatc gaagcacatc gcgaacaaca accaataaca
    11401 acaacattag aattagttga cattataaaa gaaggtattc ctgcaaaagc aagaagaaaa
    11461 ggcggacatc ctgcaaaacg agtatttcaa gcactacgaa ttgcagtaaa cgatgaattg
    11521 tcagcttttg aagattcaat agaacaagcg attgaattag tgaaagtaga cggcaggatt
    11581 tcggtaatca ctttccattc tttagaagat cgtttatgta aacaggtgtt ccaagaatat
    11641 gaaaaaggtc cagaggtacc aagaggatta ccagttatac cagaagcata tacacctaag
    11701 ttaaagcgtg ttaatcgtaa accgattacc gctacagaag aagatttaga tgacaataac
    11761 agagcacgaa gcgcgaaatt acgtgtagct gaaatactta aataaggagc aattaataat
    11821 ggctgtagaa aaagtgtacc aaccatatga cgaacaagtt tataatagta taccgaagca
    11881 acaaccacaa actaagcccg aaaagaagac tgtttcgaga aaagtggttg tacaattaac
    11941 taaatttgaa aaagttttat acataacttt gattactgta attgctatgt taagtattta
    12001 tatgctatct ttaaaaatgg atgcgtatga tacgcgagga aagattgcag atttagatta
    12061 taaaatagat aaacaatcaa gtgaaaacag tgctttacaa tctgaaatca aaaagaattc
    12121 ttcttatgaa cgcatatacg aaaaggctaa gaaacagggg atgagccttg agaacgataa
    12181 tgtaaaggta gtgcgtagta atggcgaagc aaaaaattaa aattaaaaaa aataaaatag
    12241 gggcagtcct acttgttggt ttattcggac tgctcttttt tatattggtt ttaagaattt
    12301 catatatcat gattactgga cattctaatg gtcaagattt agtcatgaag gcaaatgaga
    12361 agtatttagt taagaatgca caacaaccag aacgaggaaa gatatatgat cgtaatggta
    12421 aagtgctagc agaagatgta gaaagatata aacttgttgc agtaatagat aaaaaggcga
    12481 gtgccaattc taaaaaacct aggcatgtag ttgataaaaa agagactgca aagaaattat
    12541 ctacagtcat tgatatgaag ccagaggaaa ttgaaaagag acttagtcaa aagaaagcct
    12601 tccaaattga atttggacgc aaaggaacaa atttaacgta tcaggacaaa ttgaaaatag
    12661 agaaaatgaa tttgcctggt atttctttat tgcctgaaac agaacgattt tatccaaatg
    12721 gcaattttgc atcacactta attggtagag ctcagaaaaa tccggatact ggtgaactta
    12781 aaggtgcgct tggagttgaa aagatttttg atagttattt aagtggatct aaaggatctt
    12841 tgagatatat tcatgatatt tggggatata ttgcaccaaa tactaaaaaa gagaagcagc
    12901 ctaaacgtgg tgatgatgtc catttaacaa tcgattcaaa tattcaagta tttgttgaag
    12961 aggctttaga tggcatggtt gaaagatacc agccgaaaga tttatttgcg gttgtcatgg
    13021 atgccaaaac tggagaaatt ttagcataca gtcagcgacc aacatttaat cctgaaactg
    13081 gtaaagattt tggtaaaaag tgggcaaatg atctatatca aaacacatat gagcctggat
    13141 caacatttaa atcatatggg ttagcagctg ctattcaaga aggtgctttt gatcctgata
    13201 agaaatataa atctggacat agagatatta tgggttcacg tatttcagac tggaatagag
    13261 tcggttgggg tgaaatccca atgtcactcg gatttactta ttcatctaat acattgatga
    13321 tgcatttaca agatttagtt ggtgcagaca aaatgaaatc ttggtatgaa cgatttggat
    13381 ttggaaaatc aactaaaggt atgtttgatg gagaagcacc tggtcaaatt ggatggagta
    13441 atgagttaca acaaaaaacg tcatcatttg gtcaatcgac aactgtaaca cctgttcaaa
    13501 tgttacaggc gcaatcagcg ttctttaatg atggtaatat gttaaaacca tggtttgtga
    13561 atagcgttga aaatcctgtt agtaaaagac aattttataa aggtcaaaaa caaatcgcag
    13621 gcaaaccaat aacaaaagat actgctgaaa aagttgaaaa gcaattggat ttagttgtga
    13681 atagtaagaa gagtcacgct gcaaactatc gtattgatgg ttatgaggtc gaaggtaaga
    13741 ctggtacagc acaagtcgct gcacctaatg gtggtggata cgttaaaggt ccaaacccat
    13801 atttcgtaag ttttatgggt gacgcaccga agaaaaatcc taaagttatt gtatacgctg
    13861 gtatgagctt ggcacaaaaa aatgaccaag aagcttatga attaggtgtt agtaaagcgt
    13921 ttaaaccaat aatggaaaat actttgaaat atttaaatgt aggtaaatca aaagatgaca
    13981 catctaatgc agagtatagt aaagtgccag atgttgaagg tcaagacaaa caaaaagcta
    14041 ttgataatgt gagtgcaaaa tcattagaac cagttactat cggttctggc acacaaataa
    14101 aagcgcaatc tataaaagca gggaataaag tcttacctca tagtaaagta ctgttattaa
    14161 cagatggaga cttaactatg cctgacatgt caggatggac gaaagaagat gtcattgctt
    14221 ttgaaaacct aacaaatatt aaagtgaatt taaaaggtag cggttttgtg tcccaccaat
    14281 caattagtaa gggacaaaaa cttactgaaa aagataaaat agacgtagaa ttttcatcag
    14341 agaatgtaga cagcaattcg acgaataatt ctgattcaaa ttcagatgat aagaagaaat
    14401 ctgacagtaa aactgacaaa gataagtcgg actaacaaga atggctacat taaataaggg
    14461 tgtattgtca ctatttgttt ttactaaagt aatgctgtga tagaaaacaa catcttcatt
    14521 taatctttat aaataagtgt tgatagagaa ttttaaggta gacgaggtca gattaacttg
    14581 tttgttatag gcaaactttt tatactggcc tcttatgccg taaaaaagaa aaattataaa
    14641 ttttgattac aactttgttt att
//
LOCUS       DAKSQE010000067        14253 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.067, whole genome shotgun sequence.
ACCESSION   DAKSQE010000067 DAKSQE010000000
VERSION     DAKSQE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14253
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.067"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(18..1628)
                     /locus_tag="O0V33_001735"
     CDS             complement(18..1628)
                     /locus_tag="O0V33_001735"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCX8976363.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(1964..2494)
                     /locus_tag="O0V33_001736"
     CDS             complement(1964..2494)
                     /locus_tag="O0V33_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCX8976364.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
     gene            complement(2606..3466)
                     /locus_tag="O0V33_001737"
     CDS             complement(2606..3466)
                     /locus_tag="O0V33_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCX8976365.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
     gene            3890..4693
                     /gene="coaW"
                     /locus_tag="O0V33_001738"
     CDS             3890..4693
                     /gene="coaW"
                     /locus_tag="O0V33_001738"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HCX8976366.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLGK"
     gene            complement(4819..5490)
                     /locus_tag="O0V33_001739"
     CDS             complement(4819..5490)
                     /locus_tag="O0V33_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HCX8976367.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRLNYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLS
                     NELTF"
     gene            complement(5889..7082)
                     /locus_tag="O0V33_001740"
     CDS             complement(5889..7082)
                     /locus_tag="O0V33_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HCX8976368.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIYTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPFVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKQATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
     gene            complement(7082..8266)
                     /locus_tag="O0V33_001741"
     CDS             complement(7082..8266)
                     /locus_tag="O0V33_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCX8976369.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSDLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGP
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            8576..9046
                     /locus_tag="O0V33_001742"
     CDS             8576..9046
                     /locus_tag="O0V33_001742"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCX8976370.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
     gene            complement(9198..9548)
                     /locus_tag="O0V33_001743"
     CDS             complement(9198..9548)
                     /locus_tag="O0V33_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976371.1"
                     /translation="MNILRYLLGIGFSIIGVLHFTRERQFRNIIPKCLPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(9562..10863)
                     /locus_tag="O0V33_001744"
     CDS             complement(9562..10863)
                     /locus_tag="O0V33_001744"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCX8976372.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIDGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAIDSGAALEKFKTFIKNQGGDKTVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADYVVSPTLIHKIITE"
     gene            complement(11143..11805)
                     /gene="deoC"
                     /locus_tag="O0V33_001745"
     CDS             complement(11143..11805)
                     /gene="deoC"
                     /locus_tag="O0V33_001745"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCX8976373.1"
                     /translation="MNSAKLIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YATERLADSGVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVVKAAKGHTVKVIIETVLLDHDEIVKASELTKAAGADFVKTSTGFAGGG
                     ATAEDVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADS
                     DY"
     gene            12120..12830
                     /gene="deoD"
                     /locus_tag="O0V33_001746"
     CDS             12120..12830
                     /gene="deoD"
                     /locus_tag="O0V33_001746"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCX8976374.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            complement(12951..13394)
                     /locus_tag="O0V33_001747"
     CDS             complement(12951..13394)
                     /locus_tag="O0V33_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCX8976375.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLENAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
     gene            complement(13594..14007)
                     /locus_tag="O0V33_001748"
     CDS             complement(13594..14007)
                     /locus_tag="O0V33_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HCX8976376.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFTTLKGEVGQQFFK
                     QHPEAANKNSVILQKGDRIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FADNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
BASE COUNT         4456 a         2738 c         1988 g         5071 t
ORIGIN      
        1 cggttttatt agcaaattta tttattttgt tgatatttta atgaagcttc aatgaatgat
       61 ttaaaaatcg ggtgcggacg atttggtcta gataagaatt ctgggtggaa ttgacaagca
      121 ataaagaaat catttgtcgg aatctctacc atttctacta aacgtccatc tggacttgta
      181 ccagaaatta ccataccatt tgcttctaat tgttctctat agtcattatt aaattcataa
      241 cgatgacgat gtctttcttc aatttccgct ttaccgtaaa catcttgtgc caatgtgcct
      301 tctttaattg aacatggata taagcctaag cgtaatgtac cacctaaatc ttcgatatct
      361 ttttgttctg gtaataaatc tataattggg tatggtgttg ctgggtctaa ttcagctgaa
      421 tgtgcgcctt caaggcctaa tacgtttctt gaaaattcaa ctgttgcaag ttgcattcct
      481 aaacaaatac caaagaatgg tacattgttt tctctagcat acttaattgc actaatttta
      541 ccttcacttg cacggaaacc aaatccacct ggtactaaaa taccgtcgac atctgcaagg
      601 tattcggccg cattttcatc tgtaacttca cttgaatcaa tccatctaat atcaatatct
      661 ttagcgaaag gatatccggc atgttttaat gattcaacta cagataaata tgcatcttgt
      721 aagctaacat acttaccaac taaaccaatt gtaattttac catccaaatt attaacgata
      781 tctaacaact gtttccattc atcaagctgt gtttcatatt tcgcgtttaa ttgtaaacgt
      841 ttaataacga tatcgtccat attttgttgg cttaattgta acggaatttc gtataaagaa
      901 tctgcatcac gacattcaat aacactttct ttattaatgt cacagaataa tgcaatttta
      961 tcttttaaat cttgtgtcat ttcatattca gttcttacaa cgattaagtc tggttgaata
     1021 cctaagcctc gtaattcttt aacgctatgt tgtgttggct tcgttttcat ttctccagca
     1081 gctttaatat aaggcagtaa tgtacagtga acatacataa cattttctct acctaaatcg
     1141 ctacgaattt gacgaatcgc ttcaataaac ggtaatgact caatatcacc tgttgtaccg
     1201 ccaatttcag tgataacaac gtctgcattc gtactttccc ctgcaagtaa taaacgttct
     1261 ttaatttcat ttgtgatatg cggaataact tgaactgttc cgcctaagta atcaccacga
     1321 cgttcttttt tcaatacgtg tgaatacact ttaccggctg ttacatttga aaacttgttt
     1381 aaattaatat caataaatct ttcgtaatgt cctaagtcta ggtcagtttc tgcaccatca
     1441 tccgttacga atacttcacc atgttgataa ggactcattg tacctgggtc aacatttaag
     1501 tatggatcga atttttgaat tgttacattt agacctctat cttttaataa tctacctaga
     1561 gaagatgctg taataccctt ccctaatgat gaaactacgc cacctgttac aaaaataaat
     1621 tttgtcatgt cttgacctcc taattattaa agggaatgct tcctcatcaa ttgtaaaaat
     1681 tacgtaaaac ataacatttg agtaagctca ttcaagttgt tcatttgtcg aattcgacaa
     1741 aattaaattc tgtttactta ccgttttcat actgctatgt cactatgtat atcatttaca
     1801 actttgatta ttgcgttatc atttcttgat ttactcaatt aaataaaaaa acgctccctc
     1861 tcacaaattt tgtaagggga gcgtataaat tttatacacg tgtcctattt aaaggagccc
     1921 gaataaaatt ttatcatctt tcaactaaaa gtcaaacaaa aaattaatcg ttgaagtctt
     1981 cttcgtcttc aaacacttct tcatcttcgt ctagttcatc ttcatcttct tcaatgacta
     2041 tatccgaatg attgatttct tcttcaacct gctcatcttc tggatcattt agttcttctt
     2101 gatcatctgt ttgagctgga atatcatcgt cgtcatccat ttcatcttcg cccaataatt
     2161 ttaagttttg atcttcttca tcatctgcat ccagaatatc gaatttttga atagttggtg
     2221 cgattttctc ttcaatatca tctaccgaat accaatcacg taatccccat aaattttctc
     2281 caacatttaa aaaacgacca tctgtgttta aatctgtgta aaattgtaca acgcgatttt
     2341 caatttcttc gtactcataa tcacctaacg ctctaaactc atcgatgata tcatataagt
     2401 tcatagtttc gcctttatca ttcaataatg tataagccat atcaataaat gatttttcat
     2461 caaccatttg ttttgtataa tcttgaattt tcattgccca atacttcctt tcgaaggata
     2521 taatctaaat ttatacgtct gaattcattt atatatagta acaaaactaa attataaatg
     2581 acaatatcat atttcgcaat cactgttagt aatttaaata tttttcaaaa acgacataat
     2641 catcagtttg ttcgatttca acgaaattac tatttaaaca taatggttta aaatcattat
     2701 tctctccaaa cagaatcatc gtaatcacat taaggttgtc atagtttttt cttaagtaag
     2761 taggcaattg tcgaagcgca cttgaaccaa taccattaaa gcgatatgat ttatccactt
     2821 caatagcagc aatgttcatg cgttcaccaa tctgctccaa tgcgataaag ccaacctttt
     2881 ggccatcaat cattaaatta atgatatgta cattggtcac ttctgaacga ctttcttctt
     2941 caagctttaa tgacgaaaca tgttttgaat ctaagtccaa gtaatatagt cgttctttgc
     3001 caattggtcc ttcttcttta gtcgctgtat gcatgaatcc agcacgttcg tataggttcc
     3061 aagcattgtc attttccgca tctactacta gatatagatg attaaaatca ggaaatacac
     3121 cttgaacata ttgcggcaat gacatcatta ttttcgtgcc atatccaaaa ccttgatatt
     3181 tttcattaat cgataatgaa cgaatataaa cgacattttc aggtgtatca taaccttcat
     3241 gctgatagta acgatgtaat acaaaaaagc ccaccacttc atttttatcg tttattgcta
     3301 cgttagcaat cctatcagca tcattaattg catcatcaat aacttcttta ggtaaagatg
     3361 aatatatttg ttgtctttca ttcaagtcaa aatcattaat tgctgaacga tattgttcat
     3421 caaatgcttt taaagtgata ccttcaaaac aaagttcttt agccatatat ttaaccccta
     3481 tctttttata tatgaagcat gaatccccat attattttac ctattatttc actacgatac
     3541 cattatttgc tctaagttaa ataaattact tatttatatt ttttaaattt caaaattaat
     3601 gtgctattcc tcattactta tcattttaat cagagactta caaatacact gcgtacgaac
     3661 tacttttaat attgcgtcac taactaacaa tcctatacaa cttcaaaatt cctagaaaat
     3721 aatatatata acttaacgtc tacgtttgta atacattaat tgcgatacgc ttacaatgtt
     3781 gaaatacaat ggcatcaaat ttgttaataa ttactattat tacttgtttt ttagtcattt
     3841 tactatacta ttatttaagg atagacatta ataatgaggt gggaatctta tgaaagttgg
     3901 cattgacgct ggcggtacac taattaaaat cgttcaagag caagataacc aacgtacttt
     3961 taaaactgaa ttaactaaaa atattgatca agttgtggaa tggttaaacc aacagcaaat
     4021 tgaaaaatta tgcttaactg gaggtaatgc aggtgttatt gctgaaaaca tcaacattcc
     4081 tgcacaaatt tttgtagagt ttgatgctgc atctcaagga ctaggtattt tgttaaaaga
     4141 gcaaggccat gacttagcag attatatttt tgcaaatgtc ggtaccggta cgtcactaca
     4201 ttatttcgac ggccaatcac aacgtcgtgt aggcggtatc ggtacaggtg gtggcatgat
     4261 tcaaggactc ggttatttac taagtcaaat cactgattat aaacagttaa ctgacatggc
     4321 acaacacggt gatcgtaaca ctatcgattt aaaggttaga catatttata aagataccga
     4381 accgcccatt cccggtgatt taacagcagc taattttggt catgtactac atcatttaga
     4441 tgctgatttt acacctagca ataaattagc agccgttatt ggtgtcgttg gcgaagttgt
     4501 tacgacaatg gccattactg ttgcacgtga atttaaaact gaaaatatcg tttatatcgg
     4561 ttcttcattt cataataacg ctttattacg caaagttgtg gaagattaca ctgtgttacg
     4621 cggttgcaaa ccgtactatg ttgaaaacgg cgcattttca ggtgctattg gtgcattata
     4681 tttaggaaaa tagaaaaaga ccatttatat gcgcgttgtt ttaaatagac gctatataaa
     4741 tggcctttta ttttatggtc taagtgtttt gaatacgtat tgttccattt attatcactt
     4801 aattacaact caattcagtt aaaatgtaag ttcattagac aatttttgcg ctgaaacaat
     4861 tgtaccaacc acgccagttt ttaaatctat cgcaacggct ttatgatcat ctctaagttc
     4921 atctagccat tctccgaaaa cagaaccttc aatcgtaatt aaatcacact cttcaaaatc
     4981 ttcatctcga acttttaacg ctctgttttt aatactccat acaggcataa tatgcggctt
     5041 tttttcgtcg ctatcatttt cactaatgag aataaactgc ttactacctt ttttagttaa
     5101 gccgtatact tcatcatatt tcgctacatc ttggacaaat tctttgattc taattttatc
     5161 taattccgtc atcacttctt gtgtaacagc tacaacgttt agtagatgcc cttcttcttc
     5221 cattgtttga ttcataataa tatggtcttg ttcatcaaac aattcatcta attcataagt
     5281 agcaaaacga tcaacgtcca tttttacaac tttatcaaat ggaatatcac gacttgttaa
     5341 aaatgatacg gccacacttt cttctgtcca aacgcctaca tacgcttcac ttttataatt
     5401 taggcgtact agttttttat ttttagaggc gaaataaata cattcattta ttaatatatc
     5461 tctaaagaat ggatatgctt tataagacat gcttgaccct cactttttaa aatatgtcat
     5521 acataaaata gtaaatgact ccaacaagtt agtcaattag acatattatg aattcaattt
     5581 aatgtcatgt ttttcatttt aaaaattcaa tagcattaaa ttttacgtat acacaaattc
     5641 ggtctataac acgaaacata tctagttact aaaaatttca aaacgaaaac ctttagtata
     5701 cctttatcaa caaaatggca actcttataa catagtgaaa taaaagtcgc catttaatat
     5761 tagtattaca aagtattgcc atatcataaa tttaattact gttgactaga atcgaaaaaa
     5821 gcttgttaca aacgcatatt cagtcaacta ctggcaacac aacattgtag cgccttatgt
     5881 tccgaactct aatagtgttc aagttcagct aaaaatgcgc gttcattctc cagtactgtt
     5941 tcttttgaat caccatgcca aatacagcca aaccttgatt taactttatc ttcaccgtac
     6001 caatcaccat cataataaga taacgggtat aaactacctt ctttgacata tttcaaaatc
     6061 gtattgaaga aatgtgtgtt atcacctttt gaatgataac tataaaactt ttgatatcct
     6121 gaattcaact cgttagcaag taataacatg ctcgttgaac cattttgtct aaagtttaaa
     6181 tcaattgcat aaacgttatc atcctcatcg acgagtaggt caaaaccagc tacaccaaag
     6241 aatccttgat tcacaccgtt ttccataatt tgtctacctg cttctataac gtgctctggt
     6301 acattagtag tattttcatt tccgttataa aatccgtact tatccgttaa ttgcgttgcc
     6361 gcaccaagat attgaatacc tagcgattcc gagtatgcaa actgaacgca ataattcgct
     6421 ttttcttcaa tcttctgttc gataataagc gaattcgttt cagcagttgc ctgtttaata
     6481 cgcgttatcg ctttttgtaa atctgcatca tgataacaaa tcataacgcc atagccacct
     6541 gctgtcggaa gatcatctcc cggcttaatt acaaacggga attcccaatt cttaatctcg
     6601 ttttcgaatt gctcaatttt tacaactttt ctttttggta aaaacttccc atttgtccat
     6661 tcaggtattc ttgctttatt atttaaagca acaaataacg ttttatctaa tgcataatat
     6721 tgctgattca agattgtttc atcatgaata tattgaaaat aaatcttttt attttcctta
     6781 tgtgccaatt gtttaatcaa gttttcgtaa gattgctgat tgttaaatgt ataaattgag
     6841 ttcggtactt ccttaccaat aacttgaaat agctgatgca atttgtctgt cgcactagct
     6901 tcgtgaacaa taacaggtaa ttgatttgct attaataact ccctaccagt taaaaaatta
     6961 gattgatgtt cgtccggttt caaccatgga ttcgatatat acgaaggtct tgacgtatag
     7021 acaacatctt tgtcatataa atcacttaac gttaagttcg gctcattacc attatttgtc
     7081 attacttccc attccctttc aaatgcgcat gctcttcaat aatgtcttga taaacgtctt
     7141 gatttgtaat taactctaac cccatcaacg ccattatttt agcaccttta attaatgctt
     7201 catcaccatg tacactcgca gctgcttctc taaatctatg cgtatgtcct actaaattac
     7261 gtgatcctat tttaatatga ggatgtattg ttggcacaac atgacttacg ttccctgtat
     7321 ccgtagagcc ataaccaaaa tcatcatcaa taactgcttc accaacttct tcagcatatt
     7381 tagcaaataa atcatctaat ttcggcgttt taatgaattc attcacaccg ttttgaattg
     7441 gaccaaattc ataatcacaa ccagtctgta tcgcagctcc acgtgcgatt tgatttactt
     7501 tttctgttaa tatatccaat tctttacgcg tcattgctct agtataaaaa cgagcatgtg
     7561 tatagtctgg aataatatta gctgctttcc cgccatctaa aatcacacca tgcacacgtt
     7621 gatctttttt aatatgttgt cgtagttgtg ctacaccatt aaaataacta atcatagcgt
     7681 ctaatgcatt taacgcttca tctgcatttt cagaggcatg agcacttttt ccgtaaaatt
     7741 taacatctaa aacatccact gccaaagtat caatcgtttt ataagtttca tttcccggat
     7801 gaatcattaa ggcaatgtct atttgatcaa tcacaccagc cttgacataa gaagctttag
     7861 cgctaccatt ttccccacct tcttcagctg gacatccgag aacgactact ttaccaccaa
     7921 tttggtcaat cacttgcttc aaaccaattg caccaagcac acttgcagtt ccaatgatat
     7981 tatgaccaca agcatgaccc aatcctggca aagcatcgta ttctgctaaa aaacctatag
     8041 ctggcccgtc aagatccgaa tcatatgtcg ctataaaccc agttgcatgt ccagcaatct
     8101 cggtttcaat ttcaaaatca tgttctttca aacgatctat taaagttcga gacgcaaata
     8161 tttcttcatt accaagttca ggacgttcat gaattctatg actgatttcg atataactat
     8221 atttatttgt ttctatataa tcgagaattt gttgtttttc actcattttt tactatctcc
     8281 cttttaccct acactcattt taatcatcca caatattttg ttctttcaaa atgaattatt
     8341 acttattcta tcggttttat ctcttgatgt catctagttt ttctttattt acaaaatttt
     8401 ctaataacta aaagccttca taaatttata aaacagtttc aaattgtaaa acataagccc
     8461 tattgttaca atttaagtat tgttagaaac gcaacttgca acaaatttaa gtttaataat
     8521 gtaacgaatc ataagcgttt tatagttttg agtagttaat aggaggaaat ttaaaatgac
     8581 aaaaatgaat gttgaaagtt ttaatttaga tcatactaaa gtggttgccc catttattag
     8641 attagcggga acgatggaag gattaaacgg agatgtcatt cacaaatacg acattcgttt
     8701 caaacaacca aacaaagaac atatggatat gcctggattg cattcattag aacatttaat
     8761 ggctgaaaat attagaaatc atagtgacaa agtcgttgat ttaagtccta tgggttgcca
     8821 aactggtttc tatgtatcat ttattaatca tgataattat gatgatgtat taaatattgt
     8881 tgaagcaact ttaaatgatg tgctaaatgc tactgaggtg cctgcttgta atgaagtaca
     8941 atgtggctgg gcagcaagtc attcattaga aggtgctaaa actatcgctc aagcatttct
     9001 agacaaacga aacgaatggc atgatgtttt cggtacagga aaataaatct tagtcaatca
     9061 agttaatcag aaaagcagtc gaacaatgat gttacaatcg ccattgtcca actgcttttt
     9121 attatgcttc aaagtcaaaa aatcgaacaa atgaaaaagt aaaatcttta acatttgtcc
     9181 gatttatttg agaaccacta taatttctta attagtccca ttaacacgaa ctgcataggt
     9241 aaccttaaat atagttgcca tgttggcaat tgtttatctc ctaaaggtaa ctttttaact
     9301 gccatataga tattagctgg gaatacagct agcaagaata gattgattgt atttttcaag
     9361 cattgagatg gtcttttaat taaaagtgca agtccaaata atatctcaaa gattcctgta
     9421 acaagaaccg ctgtttttcg aagcggcaaa catttcggta tgatatttct aaattgtcgt
     9481 tctcgtgtaa aatgcaatac acctattata ctaaaaccta ttcctaataa atatcttagt
     9541 atgttcaatc agcttcaact cctactctgt aattatttta tgaattaatg taggcgatac
     9601 aacataatca gcaattgtta tgcttgaatc taatttttta acaacatcgt ctacatcttg
     9661 gcgattactg tgaatcgtta ataatgattc tccttcttct actttatcac caattttttt
     9721 atttaaaaca ataccaaccg ctaaatcaat atcatcctct tttgttaaac gtcccgctcc
     9781 taacatcatc gaagcgacac ctatatcgtt agagactaac tcagtcacat aacctgattt
     9841 tttagcttta tattcgattt gatattgagc ttgtggcaaa cgctctggat ggtcaataac
     9901 tgttttgtcg ccaccttggt ttttaataaa tgttttgaat ttttctaatg ctgcacctga
     9961 atcaattgcc tcaattagca acgctctcgc ttcttcaagc gtttcagctt tgtttgcaag
    10021 tacaaccatt tgagaaccta atgttaatac aagttctgtt aaatctttcg gaccttgtcc
    10081 tttcaacgta tcaattgctt cttgtaactc aagcgcattg ccaatcgcac gtccaagtgg
    10141 ctgattcata tcagaaataa tcgccatcgt attacgtccc acattattac caatacgtac
    10201 cattgcgtgc gctaatgctt cagcatcttc taatgttttc ataaatgcac cgctaccagt
    10261 ttttacatct aatacaattg catctgcacc agcagcaatc tttttactca taattgaaga
    10321 ggcaattaat ggtattgaat tgacagtacc agtaacatcc cttaaggcat ataatttttt
    10381 atctgcagga gttaaatttc ctgattgtcc tacaactgcc actttatttt cattaaccaa
    10441 tttcacaaat gttgcttcat ctatttcaac atgaaaacca tcaattgctt ctaatttatc
    10501 aatcgtacca cctgtatgac ctaatccacg cccactcatt tttgcaacag gaacatctac
    10561 agctgctact aatggtgcta aaaccaatgt agttgtatct cctacaccac ctgttgagtg
    10621 cttatctact ttgacacctt taatatcact caaatctatc atatcaccag aattaaccat
    10681 agccatcgtt aatgctgcac gctcatcatc gttcatatct tggaaataaa tcgccattgc
    10741 taaacttgac gcttgataat caggaatatc ccctttaaca tagccgtcaa taaagaaatt
    10801 aatttcttcc gttgttagtg tatgaccgtc acgctttttc tcaataatgt ctatcattct
    10861 catttttatc atccttttct taaaaagctt aggacaaagc atctgcgctt tctctagtcc
    10921 atttttaaaa gcacaagcga aaattattat agcaagctat cgatttcatt tttaatatca
    10981 caatttaaat gcgatgttat tattcttaaa tagattggtt ataacgtcaa agtccctatt
    11041 aaattatctt agaatcatca tggcatttat gatgtcttaa aactgatatc gacatactta
    11101 tatatggtta cgatgtccca tgcttacata ttttttataa aattagtaat ctgaatctgc
    11161 ttctaaacct tgcataattt gaacacctgc gctcgcacca atacgtgtcg cacctgcttc
    11221 aaccatttta ttgaaatctt ctaaattacg tacgccacct gatgctttta cttctacatc
    11281 agcacctact gtatctttca ttaatttaac gtcttctgca gtcgcaccgc cacctgcaaa
    11341 acctgttgaa gttttaacga agtccgcacc agccgctttt gttaattcac tcgcttttac
    11401 aatttcgtca tggtccaaca ataccgtctc aataatcact tttactgtgt gacctttcgc
    11461 agctttaacc actgcttcaa tgtcttgttg tacatcatca aaacgcccat cttttaatgc
    11521 gccgatgttg atgaccatgt caatttcatc tgcaccattt tgaattgcat cttctgtttc
    11581 aaatgctttc gttgcagttg tcgacgcacc taatgggaac cctattaccg tacaaacgag
    11641 cacacctgaa tcagctagtc gctctgttgc atattttaca tgcgttggat tcacacatac
    11701 agatttaaaa ttgtatgctt tcgcttcatc gatgatttga tcgatttgcg tacgtgttga
    11761 ctcaggcttc aataaagtgt gatcaatcaa ttttgcacta ttcattttct atctcctcct
    11821 ttatggttga ttataaaaat acggttgtaa attagttgat tgagcgtcag gttcatttaa
    11881 atatcaggtt agatgttcgc tttttatgta accgcataca tatactatta cattaattca
    11941 tttcccataa acaaacaata caattgaacg tgatatcttc attatgaacg atgacttgac
    12001 aacaagctaa tcgggattat atttttataa ttctttaatt ctatagtaca aaaattcgca
    12061 aaaaagggaa acaaatgtta tcttaaaatt attaatgaat attaaggaga agataacaaa
    12121 tgacaaaagg tacaccacat attcaaccaa atggagtaaa aattgctaaa acagtattaa
    12181 tgcctggcga tccgctacgc gcaaaatata ttgctgataa ttttttagaa aatgttgaac
    12241 aatttaacga tgtacgtaac atgtttggtt acactggtac atataaaggt aaagaagttt
    12301 ctgtaatggg ttctggcatg ggtattccaa gtattggtat ttactcatat gagttataca
    12361 acttctttga tgtagataca atcattcgta tcggttcttg tggcgcatta caagaaaatg
    12421 ttaacttata cgatgttatt attgcacaag ctgcatcaac taattcaaat tatgtagatc
    12481 aatacaatat tccaggtcat ttcgcgccta tcgctgactt cgagttagta actaaagcta
    12541 aaaatgtcgc tgaccaaatc ggtgctacta cacacgtagg taacgtatta tcttctgata
    12601 cattttacaa tgccgatcca acattcaatg atgcttggaa aaaaatgggt attttaggta
    12661 tcgaaatgga atcagctggt ttatatttaa atgcgattca tgctggtaaa aaagcacttg
    12721 gtattttcac agtaagtgat catattttac gtgacgaagc tactacacct gaagaacgtc
    12781 aaaattcatt tacacaaatg atggaaatcg ctttagaaat cgcagagtaa cttatttaaa
    12841 ttgagtttaa ttgctcttta acaatgcgat taaactcaaa aagccaacac attctgggcg
    12901 tatccccatt tatgtgttgg cttttattta tattattact tatctgtaga ttagcttaag
    12961 taagatttaa acatccaatt atgtttatct actgatgttt gcatacctat aaacatatct
    13021 tctgatacat catcgcctgc attaccagca atttcgattg cgttttctaa ttgttttgag
    13081 atatttgtga agtcttgtga taattcttca accatttgtt ctgcagagta acctttcgca
    13141 gcttctttaa caattgattg ctctaagcat tcagttaatg tacctacagg gtttcctcct
    13201 accgctaaaa ttctttcagc taattcgtct acatattggc ttgcttcatt atataattct
    13261 tcaaatttaa cgtgtaatga gaagaagtta ggccctttca cataccagtg gaaattatgt
    13321 agctttgtat aagctactgt ccagtttgct acttgttgat tcaattcttt tacaacatct
    13381 tgttgattac tcatatttaa tacactcctt aaaattgtct acgtcttgct tgtttatatt
    13441 aattattata ttataatgat tctaatatgt aaagtgtttt tactctcttt tttataatta
    13501 ttctaatcta tctgttaagt gttccgcttt ttaaatatct aaattttaat tcatcatcta
    13561 gtatatctat tatcaattag aaagtatgtt ttatcaatca gtaagtttta ctttcgcatt
    13621 atcatctaaa aagaacgatt tttcataatc atttggttgg tgccatgttg ttttccacat
    13681 tctatttcta ttatcagcaa acattcgata accgaaatta cggactggtt taggtacaag
    13741 ccataaacct acgcccaata atttagttcg attaggtagt gctgtgatta gtttaataat
    13801 cgcctgcgat tcaaaatata tacgatcacc tttctgtaaa atcacactat ttttatttgc
    13861 cgcttctgga tgttgtttaa aaaattgttg tccaacttca ccttttaacg tggtaaattg
    13921 ataatttctt ggtaggtcat gttgaattaa ccagatgaca tagttataac aatatataca
    13981 gtttccatca taatatacga ttggcataat ttattcgctc cttcaatagt acttatagtc
    14041 atttttccta atttaaaaac atttaaacaa ggttgtcact tgataagtcc tcgcgacacg
    14101 aaattgccat aaaatttatt tttcagtttt gtatttattg tataatgcat tttttattta
    14161 ctttaaattt ctcaatctac agtgtaatct tatttcttat tatcaactta gcgtttagtc
    14221 gtttgtctta ttcaacatac cactggaaaa ctc
//
LOCUS       DAKSQE010000068        14185 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.068, whole genome shotgun sequence.
ACCESSION   DAKSQE010000068 DAKSQE010000000
VERSION     DAKSQE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14185
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.068"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(198..704)
                     /locus_tag="O0V33_001749"
     CDS             complement(198..704)
                     /locus_tag="O0V33_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976377.1"
                     /translation="MKKLLTASIIACSVVMGVGIANTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
     gene            complement(840..1640)
                     /locus_tag="O0V33_001750"
     CDS             complement(840..1640)
                     /locus_tag="O0V33_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCX8976378.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAELA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSEDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLMLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDTASFINKHHDEKHV"
     gene            complement(1624..2343)
                     /locus_tag="O0V33_001751"
     CDS             complement(1624..2343)
                     /locus_tag="O0V33_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCX8976379.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKIDNKGENYGSIY"
     gene            complement(2398..3348)
                     /locus_tag="O0V33_001752"
     CDS             complement(2398..3348)
                     /locus_tag="O0V33_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCX8976380.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQIIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVIGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            complement(3498..4385)
                     /locus_tag="O0V33_001753"
     CDS             complement(3498..4385)
                     /locus_tag="O0V33_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCX8976381.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQGSDDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKKGKKQFDALNLNKEELLKAKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHEKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            complement(4704..5339)
                     /locus_tag="O0V33_001754"
     CDS             complement(4704..5339)
                     /locus_tag="O0V33_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HCX8976382.1"
                     /translation="MLGTDELYKLLYRHMGPQNWWPADNDIEMMLGAILVQNTRWRNA
                     EIALNQIKEHTHFNPNHILELPIETLQSLIRPSGFYKSKSLTIKTLLTWLARHHFNYQ
                     EINERYKGELRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYNKLGYENTKS
                     YDQLKKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNCDFLEPYFKK"
     gene            complement(5817..7478)
                     /locus_tag="O0V33_001755"
     CDS             complement(5817..7478)
                     /locus_tag="O0V33_001755"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCX8976383.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVAAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            complement(7475..7891)
                     /locus_tag="O0V33_001756"
     CDS             complement(7475..7891)
                     /locus_tag="O0V33_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCX8976384.1"
                     /translation="MDKKVSIHTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLEVKTLRILH
                     FVSGYGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            complement(7984..8092)
                     /locus_tag="O0V33_001757"
                     /pseudo
     CDS             complement(7984..8092)
                     /locus_tag="O0V33_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8167..9177)
                     /gene="adhP"
                     /locus_tag="O0V33_001758"
     CDS             complement(8167..9177)
                     /gene="adhP"
                     /locus_tag="O0V33_001758"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCX8976385.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
     gene            complement(9676..10197)
                     /locus_tag="O0V33_001759"
     CDS             complement(9676..10197)
                     /locus_tag="O0V33_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HCX8976386.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKHSSDALHQAFN"
     gene            complement(10225..11520)
                     /locus_tag="O0V33_001760"
     CDS             complement(10225..11520)
                     /locus_tag="O0V33_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCX8976387.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVSPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGKIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            complement(11560..12036)
                     /locus_tag="O0V33_001761"
     CDS             complement(11560..12036)
                     /locus_tag="O0V33_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCX8976388.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYEPEMYGEKLDLALMYKAF"
     gene            complement(12205..12741)
                     /locus_tag="O0V33_001762"
     CDS             complement(12205..12741)
                     /locus_tag="O0V33_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCX8976389.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQDLEYQWIDVTQHQFKPIRDV
                     RHTAETITSYDDDYLPILDKILASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTAEKPGDI
                     MKDNYALARATEWNSILQ"
     gene            complement(12951..13889)
                     /locus_tag="O0V33_001763"
     CDS             complement(12951..13889)
                     /locus_tag="O0V33_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCX8976390.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDDNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFKPGVFEENVRRVKALESIAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVIENIKAADIVLSDDEIQYIDELFPIED"
BASE COUNT         4446 a         2711 c         1857 g         5171 t
ORIGIN      
        1 tatattttct agtaaaattt tatcgttaat acgcattggt agtactcctt taaaatgaaa
       61 catctttatt tattgtattg gtttaatatc cccttcgcaa atcataaaaa ttaatattca
      121 ctgtattatg ccatgtaata aaataaccga caagtattgc gatttaactt gtcggttaag
      181 cacgattaat ttaattatta tttttccaaa tcaatacgat ataattttat gtttttatct
      241 tttggtgctg aggcaaattg ataagtcgtt ttgccttttt caatatatcc aaaattacca
      301 gcaactttgc tatattccgt atgtgttact gctttgccaa cataattttt aacagcttga
      361 tatgttggac cattagcatc attatacatc attgaaatat gagttacttt accattgttt
      421 ttcttaccat cagtagttac taataacata ccttttttag tatggaattc gtaataatgt
      481 tcagttccat cttcattgta agaatacatt ggatttttgt acttagttaa aacatcttta
      541 atagattcgc caattttcac attttctaat gactggtcac ccttaattaa ttgtttaaca
      601 gtatcaatag agttgccact tgctgcttcg gcactagtgt tcgctatgcc tactcccatt
      661 acaacagaac atgcaattat acttgccgtt agtagctttt tcataagcac tctctccttt
      721 tatttatatc gtcttgtgct cacaaccatt atacaacaat catttctaaa acaacagtca
      781 tattacaatt atattacaaa taataattac tacttttata tttcacttat cactaaaaat
      841 taaacatgct tttcatcgtg atgtttgttg ataaatgaag ctgtatcttc aacgaatctt
      901 tcttgctctt caacaaatgg gaatgcactt gattcttgat atacttcaaa gtccgcattt
      961 tggattaaat cagctacttc tttagcttct aatcttgttg ttctttcacc gaattctcct
     1021 gcaataattt ttgtcggtac tacgacattt ctataagttt gagaaatatc agcgtttttg
     1081 aatacctctt taactgcttg aatttcttct ttagttgata tttcatttgt atctacgaca
     1141 tgtttgagga atcgattcat ttttctcgga cgataatact tacgcttatt taaaaattta
     1201 tcttgttttt caggatccca gtttcgaata atatgggcat attttctaaa taaacgttct
     1261 tccggtaatt caccttcaat agatgttgga tttaccaaca taagtgatga tgtaaattca
     1321 ggataacgta ctgaaatatc cgcaccaatg attccaccca tttcatggca tacaaatgca
     1381 acttcttcaa tgtatagata tttaagtaat tcaacaatat cttcagaaaa atcttttatt
     1441 tcaatgtgac gaggtttatc agaatagcca tgtccacgta aatcaattaa cacaacttga
     1501 aatgattttg ctaattctgc tgctaattta ttaaacacag aataattatc aagtacagta
     1561 tgaatcaata cgataggata gccttcacct aatgtactgt aatgtatcga tgttccatct
     1621 tttctagtaa atagatccat aattttctcc tttattatca atttttattg gtaaaatggt
     1681 taataattct tccactgttg taattgtaaa atcaacttct tcttcgagtg gttcaatttc
     1741 tgcatcttct tgtttatacc atacactaac catgcccata gctcgtgctg gagctacatc
     1801 attcaatgca tcatcaccga catacataat ttgctcaggt aataccccta gttgatcaat
     1861 catatcttca aaaattttag gatgtggttt acgaaaacca actgtttctg atgttgataa
     1921 ataattaata acatgcatca aaccaagtga atgtaatcga aattgtttaa tcttagattt
     1981 accatttgcg ataacaccaa tttgaaagcc tttttgcgat agcttttcta gtgtatacaa
     2041 agtatcgtaa taaggaaata cataacgata aaaatgcatt tcaaaatcgt taaataagtc
     2101 tttccaagtt aacctatcta catggaaacg tttaatgatt tctttatata aatcaggttt
     2161 atcattatct tcatcgtcat ctagctcaat aaatgctttt ttaaaatctg ccagttgaac
     2221 atgaattaag tagtcatgaa atcgttcata ttgctcttcg ataaatttct ctcgagattt
     2281 aactctgtcc aacaacgtac cttctaaatc aaatacaact gctttaattt gattcaaatc
     2341 catcgcttat gccctcttta tgtttatagt tatttttatt ttagcaaatg tcatgtgcta
     2401 gtgtaaccgt ttcgactgag atattagcat gtaaaataac actggtatac caattaatgc
     2461 aatgataata cttgccggta tttgtacggg tgacaatata tggctaccaa taaaatctgc
     2521 cataaccatt atttgtcctc caattacaat attcaaagcc aattgtcttc ctaatgattg
     2581 tttccactgc cattttctaa tgagttgtgg tatcaccata ccaatgaatc caattatgcc
     2641 aacatatgcg acatttatcg ccgttatcat agaagctata cataacgcga tatatgtaat
     2701 tatttgaaca tttaagccta gtgactgact ttttagttct cctaattgca ataactttag
     2761 ttgattaaga atgataaata taccaaacag tgcaatgata aatgtgattg ctattatgga
     2821 tacatttgag tattctgcat caccaaaacc accaaacaga taattggcaa ttgtatttaa
     2881 tttgcgaggt tttaataaaa tcaaaaaata tagaagtgaa ttgaatagtg caccaatcat
     2941 taaaccactt aatattaaga ttctaactgg atagccttgg ctcaataccg acgtaatgac
     3001 taatacagtt attaatgtta ttaaactaaa tgttattgaa aatacaggaa tccataacgt
     3061 tgttaatcct aaaaataatg ctaatcctga accaaatgta gcaccgcttg ccaatcctaa
     3121 tgtaaagcta tctgccaatg cattatttaa aactgtttga aatataagac ctgcaacagt
     3181 taatatcata cctgtcaaca atgcttctaa taatctcggt aatctaacat tgattaaaat
     3241 agactggtta aatacatcat tgatatcccc caactgccaa aatagatata tgctagttgt
     3301 tataatcaat atgactatcc agctcaataa tactttatta aatgtcatag cattctcttt
     3361 tctttcatcg ataattacat tcattatagc aatcacatac tcgcgttgct atcattcact
     3421 aaatatattt taaaacccct tatctcaaac atgtaatttt tattacatgt agcaataagg
     3481 ggcataatta gaatggttta ttttctataa attgcatctc ttaattcttt taatccttca
     3541 tcaatacgtg gacccggtct tgatacttca tcaccattta caacttcaat acgtgtattc
     3601 ttgacagcat taattttatt gaatccacct ctttttttga tgatatccat ataatctgat
     3661 cttgtcttag cttccgtcga aattaatata tctgggttct ttttaataat actttccttc
     3721 gttacagggt tccaaccatt aatgtcacta taaacatttt gggcttctaa tttttctaac
     3781 atatcattga aaaatgtatg cttccctgct gtatatattt caggctttga tgaaacctca
     3841 ataaatactt ttgatttttt atgatgagca ggaattgaat ctatgacttt atcgatatta
     3901 tccttagttt cctcaacaag ttgttcagcc tgcttttcat gatgcgttaa tttcccaatt
     3961 tgcttaaatg tgttgtaagt ttcatcaatt gattgtgcat ctttaacata cactactttg
     4021 atgccttgtt tctctaatga tgacaatact ttattagcag ttgccttttg cgactcatgc
     4081 gcaagaatta gatctggctt tgcctttaaa agttcctctt tatttagatt caaagcatca
     4141 aattgtttct tacccttttt cacatctttt ggataatcat caaccgttga aacaccaact
     4201 atgtatttac ctaatcctaa ttcatataaa atttcagtat tactaggcat taacgaaaca
     4261 attctatggt acggcgattt agacgagcca ttatcgtcgc taccttgttt atcattatct
     4321 ttcataccac agccactcag aacaagcata aaaatcaaaa taaaagcaat taacgatttc
     4381 ttcacattat cacatctcct ttttaaagcc tcttgaactt tagtgattag aggcttttat
     4441 gcagttgtgc ggttacatcc aactgcgttc ctttaatccc acttttcgct ttagcgatta
     4501 gagggattta tgcagttgtg cggtagcatc caactgcgtt cctcttcacc tttagtcatt
     4561 agaggctttt atgcagttgc tgcacaaaca aacaactttt ttctttatat gcttctattt
     4621 attatataag tccacatact aaaagaaaat agtaaattat tttttatatt tcatataaac
     4681 tcatctatct aacttcacag cgtttacttt ttaaagtaag gttctaaaaa atcacaattc
     4741 tttatatctt tatctctaaa gtaatgttta ccaaatacat ctaacagagc atgaaattca
     4801 ttagcatctt gatttgtaaa atgatttggt aatgtgacta ctttttttaa ttgatcataa
     4861 cttttagtgt tttcatatcc taatttatta tatatttttc ttgtatagct atctggaata
     4921 aattcaatac gtccgaatat ataaacaagt aagacatctg ctgtttcact tccaatacct
     4981 ttcaaagata ataattcttt tcttaattca cctttatatc gctcattaat ctcttgataa
     5041 ttgaaatgat gtcgtgctaa ccatgttaat aatgttttaa tcgtcagtga tttactttta
     5101 taaaagccac ttggacgtat caatgattgt aacgtttcaa taggtagttc taatatatga
     5161 tttggattaa aatgcgtgtg ttctttaatc tgattcaatg caatttctgc atttcgccat
     5221 ctagtatttt gaactaaaat tgcacctaac atcatttcaa tgtcattatc agcaggccac
     5281 caattttgag gtcccatatg cctatataac aatttataca attcatcagt tcctaacatt
     5341 tacatcctcc ttttagagcc ccttgagctt tagcgattag gggctctttt gcagttgctg
     5401 cgcaagcaaa ccaactggat tcctttaacc cccttgagct ttagcgatta ggggctcttt
     5461 tgcagttgct gcgcaagcaa accaactgga ttcctttaac tccccttgag ctttagcgat
     5521 taggggctct tttgcagttg ctgcgcaagc aaaccaactg tattccttta acccccttga
     5581 gctttagcga ttaggggctc ttatgcagtt gtgcgcaagc aagccaactg tattcctttc
     5641 cccccttgag ctttagcgat taggggctct tttgcagttg ctgcgcaaac caactgtatt
     5701 cctttaacct tattcacata ctaagcattc cccaatatgt cacataccaa ataaaaaaac
     5761 actccaacac aaagtatctt tcgagaaact ctacgttgga gtgtatataa atgttcttac
     5821 attgattcag gtgcgcttac accgaccatt gccaatgcat ttttcaatgt aattctgacc
     5881 gcttcaatca tagcaacatg tgcttttgtt ttttcaatat catctgttaa cactttttca
     5941 gcattataga atttatggaa atgagaagct aaatcttgaa tataattagt aattctatgt
     6001 gctgatctat gctcagcagc actttcaatt gtaggttcga aatcagctac ttttttcaac
     6061 aattcgatcg ctttttcatt tgtaatcgtt gtaaaatcat tcgcagcagc tacctcaatt
     6121 ccttgctctt tcgcttgttt taaaattgaa caaatacgcg catgtgcata ttgagcatag
     6181 taaactggat tgtcttgaga ttgctctttc gctaattcca tatcaaaatc aaagtgacta
     6241 tcaggactac gcatagttaa gaaataacgt gcagcgtcaa cgccaacttc atccataatt
     6301 tctcttaatg taatcgcatt accagtacgt ttactcatct tcacttcttt accattttcc
     6361 attaaacgaa ccatttgcat gatttgaatt tctaaacgat tactatctac accaaacgtt
     6421 tcaagagatg ctttcaaacg gttaatataa ccatgatgat cagcaccaaa taaatcgatt
     6481 aaaatgtcat taccacgttt aactttatcg aagtggtacg caatatctgg taagaaatac
     6541 gtatatgtac cgtcattttt aattaatact ctgtctttgt cgtctttaaa atcagttgta
     6601 cgtaaccatg tagcgccatc agcttcatac gtataaccta attctttcat tttagctaaa
     6661 acttcaagaa tttcgccttt ttcatataaa gatgtttcac taaaccaatt atcaaaatgc
     6721 gtattgaact ctgctaaatc atttttcagt ttagccattt cgtattctac gcctaatttt
     6781 ctaaattctt tcaaacgtgc ttcttcagaa taatctttaa tttcagggtg tttctctgct
     6841 aaatctttac ctatttcaat aatatctttg ccattatagc catcttctgg cattgaataa
     6901 ctattgtcac ctaaagcttc aaagaaacgt gtttcaatcg aacgcgctaa gttagtaatt
     6961 tgattaccag catcattaat ataatattca cgtgttacat tatagccagc tgcagttaaa
     7021 atattagcta aagcatcacc aactgctgca tttctagcat gaccaatatg taaatctcct
     7081 gtagggttag ctgaaacata ctcaagcaat acattttgac cttttgattc atttacatgt
     7141 ccaaattgat cacctttttc aattgcttca ggaataattg ctgttaaata ctgattatct
     7201 aagtaaaaat taatgaatcc tggaccagca atgtcaattt gttttacatg tgctttttca
     7261 gtatctaagt tatcaacaat cgcttgagca atttcacgag gattacgctt tgcaatctta
     7321 gtcagtacca tcgcaatatt agtagcataa tctccatttt ttgtatcttt aggaacttca
     7381 attttaatat caggaatctc atctgctaat cctgctttgt taatacttgc tgcaatttct
     7441 tctactaatg tttgtttcac ttgatcaata atattcattc gcctatctcc ttatagttaa
     7501 tttcatattg ataagaaccc attttttcat tatcttgata taattcataa tgaatcttta
     7561 acttgccacc gtatccactt acgaaatgta aaatgcgtaa tgttttaact tctagtggaa
     7621 ttcgtccagc cgatatatcg taaaaagttg ttgtcgtttg tccttcaaca aaatgcaaat
     7681 tcatattaat gtcgccttta cgaatcaact taacgctatc atcatccact tttattgtaa
     7741 cattaactgt tgcatcctca atttgttcta cataccgaat aaagttagat tgcctttgtt
     7801 gccaagttcc ttcagtagta aattcaaatt tttctttttc gttgtgctgt ttcaacactt
     7861 gctttgtatg aatacttact tttttatcca atttactact caccttaatc ttcgcataat
     7921 agaaatatta tagcataaaa taaggtcatc ttgttatgcc ttcttaaagc taaataggtt
     7981 caattaatta acaattccat acttaatcaa cgcttgatat atgtaagcca ttttataaaa
     8041 tacaaacaaa acctcaaaac caaaaaaaca cgagattttc actatctttc atgaaaacct
     8101 cgtgtttgat tataatgata gtgttcagag cactaccttc tttatctata aagtcatttg
     8161 atatttttaa aatttaataa ccattctacc agttatagta ccattttcca tttcttcaaa
     8221 aatatcattg atttcttcta attttcttaa ttgaacttta ggtgttactt tattttcagc
     8281 agcaaattca aacgcttcac gtaagtcttg tcttgtacca acaagtgaac ctactacttc
     8341 aataccatct agcactaatc ttgggatatc taagttcatt ttatcaacag gtaaaccaac
     8401 ggcaacaact cttgcaccag cttttacaac atcaaccgct tggttaaatg gcgtcttagc
     8461 aactgaagtt accactgttg catctaatcc tttgttatca gttaatttca taacttctgc
     8521 aactggatca acatctttag aattaataat agcatctgca cctaattctt ttgcgaatgc
     8581 taatttatca tcattaatgt cgaaggcaac aattttagcc cccataacgt ttttagcata
     8641 ttgtaaagct aggttaccta aaccacctat accaaaaaca cctaaccatt gtccaggttt
     8701 aacattactt acttttacag ctttataagt tgtcacacct gcgcatgtaa tagaagacgc
     8761 tgctgctgga tctaattttt caggtacttt cacagcatag tctgcagtaa caataacttg
     8821 ttcagccatt gcaccatcta ctgtataacc agcatttttc acactacggc aaagtgtttc
     8881 acgacctgtt gtacaatatt cacatcttcc acagctttcg aacatccaag cgatagacac
     8941 acggtctcca atttttaatg attctacatc ttctgcaact tcgatgactt taccaatacc
     9001 ttcatgacct aaagtaacgc ctgtaacatc accaaaatca gcattcttaa catgtaaatc
     9061 ggtatgacaa acgccacaat attccgtttg taccaatgct tcaccaggtt ttaaagctct
     9121 taactttttg tcctcaatac ttactttgtg atctttcgtt acaactgctg ctctcataat
     9181 aatgtcctcc ttatagtcac tccagtatgt atgatattaa ttggtgatta ctttcaaaaa
     9241 tgaaaacgaa taattataga tgtaattttt atttgtaata gacgccaaat taaaacatac
     9301 tattgtgact ttgtttttag ttagatatca accgttaact tttaacgttt ccctagttaa
     9361 tatgttaaaa gaaatgacat aatcgatatg ctaacgtgta atttttactt cacatatatt
     9421 tatttaaaga taatttataa tactcttatc ttcactctta ttataatgtg aattaattca
     9481 caaagtatat agctatgatc aaaaaaatgt gtcagttatt caaatttttt attaactaca
     9541 gattaattta atttcaaaaa aattaaaaaa actcccaatc aatctaagac tttattttca
     9601 cctcaagatt tacgtcgtca ttgagaaatg aatcatatct ttatagatta attgggaaat
     9661 tttagtttct aatatttaat taaatgcttg atgtaatgca tcagaagaat gcttccacat
     9721 accttcatca tggttcatta aaaagtcttt taaaatagct ttattctcgt caccaatttg
     9781 atcaatgata atatgatgtt tcattgattt atccatcata ttaacatgtt caggcatact
     9841 tttataacca cgacgaattg ctttattcac taataaatag cacgctgtta aaccagcata
     9901 atatggtcct tgttcattac gttctgttgt tacccaacac aaccaatatt ctttagcttc
     9961 atcgccttca acagcagctt tatcagtaat ccatttcacg cctttttcaa ctttggcgcg
    10021 agcatgcatg cctccaatat caataaatgc ttctttgtta gcaacatcga taaaaactgg
    10081 tgcaatattg tctaagctaa ttgaaccaat atttgtacct ttatgaccat ctagagggtc
    10141 attttttatg atattaaaat taaagccttt tttctcagac aatgatataa cctccatatt
    10201 taacgataac aattttagcc ctgtttaatt aagctcatta atcaaatttt caatagcttc
    10261 tctaattgtc ttatctttaa tctttgcaac catttctcta ggataatata atttatagtc
    10321 ttggcgatta atgcctgtaa tactatgaat gactaaagat tgattgctta tttctctaat
    10381 cttaccatct tgtcttaata aatgaatcgg tttgcgattt gacccaggtc gatcatagtc
    10441 atatggcaaa tccgaaaatg cttcactcac aaaataataa tctggattaa taccacctgc
    10501 ttcaaacagt tcttgcagtt ctgatattgt aataattgag ccatcaaatg gaatatattt
    10561 aaataagtct cgattaataa atcgacttgc taaatcactt aaaatggcat catcttcttt
    10621 tgtccatttt tccaaataat acgtaactac cgcttcatcg agttcaacat attgttcaat
    10681 cgtaactgtc tcttcaaaaa atggaataaa atcatgtgga tgcaacttaa attcatagcc
    10741 ttcattataa agctgttttg cgcgtttcaa acaattatta agcagcactt ctccaccacg
    10801 actaactggg tggaaataaa tctgccaata catttgataa cgactcataa taaagttttc
    10861 aactgcatgc ataccacttt ctttaattag tacttcatct ttagaaggtc gcattaatct
    10921 taaaatacgt tccatatcaa aagcaccata tgacacacct gtgaaatacg catcacgttg
    10981 taaataatcc attctatccg catcgatttg tgaagaaatc atcgaaatga ccaatttatt
    11041 atgatgcgtt ttattaatta cttccgcaac ttctcttgga aactcaggcg atactttacg
    11101 taatacagca ttcacctcag tatctccagt aataatcgct tgagtgtatg cttcatggtc
    11161 tgtattaaat attttttcaa aactatgtga aaatggacca tgtcctaaat catgtaataa
    11221 tgcagcacat aatgccaacg gtctatcttt attgtcccat gcatcatgac caataaatga
    11281 ctcatcaatt aatcgtctaa ctatttcata cacacctaaa gaatgtccaa agcgactatg
    11341 ttctgctgtg tgaaaagata ggtacagtgt tcctaattgt ctaattcgac gtaacctttg
    11401 aaattcctta gttttaatta aatcccatat caattgatct tcaacatgaa tatatcgatg
    11461 tatcgggtct ttaaaaactt tttcttctac taattttaaa tctacatatg cgttagtcat
    11521 tattcccctc cttttcgttt aatataatat ttaatttact taaaatgctt tgtacataag
    11581 tgctaagtct aacttttcgc catacatttc tggctcataa gagcgtaaga ttgtaaaacc
    11641 ttgctcttta tagtaagcta ctgcttcttc atttttatta tctacttcta agtaaacacc
    11701 ttcaaattta tcttcaaaac gtgataatcc ttcatttaac aatgctgtac cataacctgt
    11761 atgttgcgat tctggtttaa cataatgagc tgataaatat aattcttcac cgtaaataaa
    11821 gttagcaaag ccaacgatgt cattaccttc ttcaacgact aagaataatt gttcttgaag
    11881 tcttttcttt aaatgatgtt cattatatga agcttctaac aagtgattaa ctgttgtcgc
    11941 agcgtatata tttaagtatg tattaaacca agctttagtt gcgacatctc taatttgaac
    12001 aacatctttt tcagttgctt gtcttacctt gaacatgact ttctcccctt attaacaagt
    12061 tttaataacg gcattatacc acaacttgct caatacttaa taaacaatga ttgtctattc
    12121 aatttatata tttatatttt ccgttaaaat taaaaataaa aaataacgaa gcaaaaaatc
    12181 acttcgttta gtatgaggta tgtcttattg caatatacta ttccactcag ttgcacgtgc
    12241 taaggcatag ttgtctttca tgatgtcacc aggcttttca gcagttccaa taatataacc
    12301 atttaaagtg gcacctaaaa agtctaaact atatttcatt tgcgtaattg ctggcttcgc
    12361 ttttattttt ggacaatctc caccaactaa aataactcta aaatcctttt cggccatttg
    12421 tgccttaaaa ttaggatatc gtttatcttg taatgtttct gaccaatgtt cgataaatgc
    12481 tttcaatggt gctgaaatgc tataccaata cactggtgat gcaaaaataa tcgtatcact
    12541 agccaatatt ttatctagaa tcggcaaata gtcatcgtca tatgaagtaa tagtctctgc
    12601 tgtatgtctc acgtcacgta tcggtttaaa ctgatgttgt gtcacgtcaa tccattgata
    12661 ctctaaatct tgcaaagcga attttgttaa ttgtgcagta ttaccgtttg gtctactccc
    12721 accaaacaaa acagtaatca ttttagccta acctcacttt tgattaataa atatctgtgt
    12781 ttttcgttac ctaattatac tatcataagc tttacctacc gaatagtaaa acgcttacaa
    12841 cttttatata aatttgacga aatttcgtca tgccttatat aaagtcgttt gtgatacggg
    12901 gctaattcat gatgaattag atacatatgt caccattaaa tacaattcat ttagtcttca
    12961 attggaaaca gttcatcgat atattgaatc tcatcatctg ataaaacgat atctgcagct
    13021 ttaatatttt caatgacttg ttctgcacgt tttgcaccag gaataatcac atcgatagct
    13081 ggtctcgtta aataaaatgc taatacaatg ttcgcaattg aagtttgatg tgctgcagct
    13141 atgctttcca aagcttttac gcgacgcaca ttttcttcaa atacacctgg tttaaaatca
    13201 cgacgtgtag tacgatggtc actaaattta gtgttctcat catattttcc agctaaaata
    13261 ccggatgcta aagggaaata tggaataaat gtgatttggt gatcaacaca atattgtaat
    13321 actgcctcat tttcacgatg caataaatta tattctaact gtacaacatc aacgtaacca
    13381 tctttatttg cttctttaag ttgatctaat gtgaaatttg atacaccaat ggctttaatc
    13441 ttcccttgtt ccttaagctc ttgtaatgct gcaactgctt gatctttcgg agtgttgtca
    13501 tctggaaaat gaatataata taaatcgata taatcagttt gtagacgttt caaactattc
    13561 tcaacttgtt gttttaaata ttccggttga ttgttctgat gtacttcttg attttcatca
    13621 aattcatgag accctttcgt agcaatttta atttgctctc gcggatattc cttaacaact
    13681 tctccaacca attcttctga tcgttctggc ccataaatat atgccgtatc taataaatta
    13741 ataccatgat taatggcttg acgaacaaca tcttttcctt gttcttcatc taagttcgga
    13801 tataaattat gcccacctac tgcgttcgtc ccaagtgcga ttggaaacac ttcaacatca
    13861 gatttaccta agtttacaaa ttgcttcatt agacccagct ccttatcttt ataacttttc
    13921 cactatcaca ctatcatgat ttaatacaac ttccaaatgt tagaattagg gtttgaattt
    13981 aggcggtgta ttagaaaaat gttatgtaga actcagttaa aataagtatt gcttaagttg
    14041 atattcacat agtgcgattg cttgttagcc tttaaattac atgtactttt gaatcagaaa
    14101 ttgaaaaatc atttaagaac gacaacatta aagccattgc aaattcaatt agaaatacta
    14161 taatgttttg atttgcataa caaag
//
LOCUS       DAKSQE010000069        14063 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.069, whole genome shotgun sequence.
ACCESSION   DAKSQE010000069 DAKSQE010000000
VERSION     DAKSQE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14063
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.069"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(42..227)
                     /locus_tag="O0V33_001764"
     CDS             complement(42..227)
                     /locus_tag="O0V33_001764"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCX8976391.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            375..1637
                     /locus_tag="O0V33_001765"
     CDS             375..1637
                     /locus_tag="O0V33_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCX8976392.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVVLAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHNQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYS
                     KGIVSKAVSFTEAGTKHGRLGLMAGHKM"
     gene            complement(1773..2864)
                     /locus_tag="O0V33_001766"
     CDS             complement(1773..2864)
                     /locus_tag="O0V33_001766"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCX8976393.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIHMPTKPGVIVSDTGSTKAMI
                     QQHECSLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNSQEHHLVNKLAAGGF
                     RDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAEA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            3029..4060
                     /locus_tag="O0V33_001767"
     CDS             3029..4060
                     /locus_tag="O0V33_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCX8976394.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGD
                     FVSFDPRTVITSSGFIKSRHLDDKASVAMILKLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDSSGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            4553..5959
                     /locus_tag="O0V33_001768"
     CDS             4553..5959
                     /locus_tag="O0V33_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCX8976395.1"
                     /translation="MDVFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSIVIF
                     DIYGTLTLDNDVLSVSTLKESYQITERPYHYLTTKINEDYHNIQDEQLKPIPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVNKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHANQHLNQLTFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITKLMVIEKYEHVMHIVSEVTGEINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVSP"
     gene            5956..6522
                     /locus_tag="O0V33_001769"
     CDS             5956..6522
                     /locus_tag="O0V33_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCX8976396.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIIQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQHLMKIISSYQDKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTL
                     KVISHHQHLLYQDVPEQFSIMRYHSLISNPDNFPEELKITGRTDDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINYVKKG"
     gene            6528..7524
                     /gene="trpD"
                     /locus_tag="O0V33_001770"
                     /pseudo
     CDS             6528..7524
                     /gene="trpD"
                     /locus_tag="O0V33_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
     gene            7526..8308
                     /gene="trpC"
                     /locus_tag="O0V33_001771"
     CDS             7526..8308
                     /gene="trpC"
                     /locus_tag="O0V33_001771"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HCX8976397.1"
                     /translation="MTILEEIVEYKQLLLQNGYYQDKLNTLKSVNIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYEQYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LVEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKPNHYYISESGIH
                     DVSDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            8308..8940
                     /locus_tag="O0V33_001772"
     CDS             8308..8940
                     /locus_tag="O0V33_001772"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HCX8976398.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPDHIDKVCVVVNPDLTTIEHVLSNTPINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKNIKDIPYLIAGG
                     INTENIQTVNQFKLSHQGFDLASGIEVNGRKDIEKMTAIVNIVKGDRENE"
     gene            8933..10147
                     /gene="trpB"
                     /locus_tag="O0V33_001773"
     CDS             8933..10147
                     /gene="trpB"
                     /locus_tag="O0V33_001773"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCX8976399.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGEDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEGGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMAERGLAND"
     gene            10140..10871
                     /locus_tag="O0V33_001774"
     CDS             10140..10871
                     /locus_tag="O0V33_001774"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCX8976400.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFEQLEKHGNQIKCQYVLMTTYYNIICHYGEQAFFEKCRD
                     TGVYGLIIPDLPFELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSYAEGFIYTVT
                     MNATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRSPQHVADIKEVADGIVIGSEI
                     VKRFKSNTREEIIKYLQSIQQTLNN"
     gene            11193..12455
                     /gene="femA"
                     /locus_tag="O0V33_001775"
     CDS             11193..12455
                     /gene="femA"
                     /locus_tag="O0V33_001775"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HCX8976401.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRYLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKERVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
     gene            12474..13733
                     /gene="femB"
                     /locus_tag="O0V33_001776"
     CDS             12474..13733
                     /gene="femB"
                     /locus_tag="O0V33_001776"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HCX8976402.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKKLKK"
BASE COUNT         5123 a         2004 c         2593 g         4343 t
ORIGIN      
        1 ttcgttttca aagaattaat tggcatgcat cttatgaaga attattgatc tgactttctt
       61 acgccagcca caccataatg gtttggtttc atttcttcta taacaacgtg aattgcttgt
      121 ctatttgctc ccgttgtttt ttctacggcg tcagtaactt cgctaactaa attttttaat
      181 tgttcatccg aacgaccttc taataatttt acattgacga ttggcatcat ccaaacctcc
      241 taatcattat taatttgagt atatcatgta atacttaaaa attcatttgt aaaataatag
      301 aacatttgtt ctaaaattgt tgaaatagaa caagtgttcg tatataatgg aattcaagta
      361 agggaggaat aacagtgtat aattatcatt tattagaaga tagggatgtt ctatgtattg
      421 atcaaaaaag tttttttgcg agtgtttctt gtattgaaaa ggggctagat ccattagaaa
      481 caaagttagc tgttgttgca gatactaagc gtcagggttc tgtagtattg gctgcgacac
      541 ctaaattaaa agaattaggc atcaagacag gatcgcgatt gtttgaaata ccacatagaa
      601 atgatattta cattatcaat ccaagtatgc gtaaatatct taatgtttca gttgctattt
      661 ctaagattgc attgcgttat attccacctg aagatttaca ccaatatagt attgacgaat
      721 tttttatgga tgttactgat agctatcata gatttagttc tacagtacat gcattttgcg
      781 aaagacttaa acgtgaaatt tatgaagaaa caggcattta ttgtactgtg ggcattggtt
      841 ctaatatgtt attaagtaaa attgctatgg atgttgaagc taagcataat caaaatggta
      901 tagctgaatg gcgatatcaa gatgtaccaa caaaattatg gccaattcag cccttgagag
      961 atttttgggg tattaatcgt cgaacagaag ccaaattgaa taaaagaggg atttttacta
     1021 taggagattt agcaaaatat ccatataaat ttttaaaaaa agagttcggt attttaggtg
     1081 ttgatatgca tctacatgcg aatgggatag atcagagtaa agtacgtgaa aagcataaga
     1141 ttagcaatcc atcgatatgc aaaagtcaaa tattaatgag agattatcat tttgacgaag
     1201 caaaagtagt aatgcaagag ttaattgaag atgttgctag cagagttcga gcaagaaaaa
     1261 aagtggcaag aacgatacat tttgcctttg gctatagtga tgaaggtggt gtacataagc
     1321 aatatacttt gaaagatcca acaaacctag aaaaagatat ttataaagta gtaatgcatt
     1381 tcgcagataa attatgtaat aaacaagcac tatatcgtac gctaagtata tctttgagtc
     1441 aatttattaa tgaggatgag cgacagttaa gtctgtttga agatgaatac caacgcaaac
     1501 gtgacgaatg tctagctaaa acgatagacc aattacattt gaaatacagc aaaggtattg
     1561 tgtccaaagc agtatcgttt acagaagcag gtacaaaaca tggtagatta ggtttaatgg
     1621 ctggacataa gatgtaatga ctatacggtt taaggaatat ataactgtga ttcgtataaa
     1681 ataagtctct aaagataaat atttcatata tcacaataga tttcacaata atatctaaga
     1741 atacatggaa tttatcaaaa gagactcaat aattattgga tataacaatc aaaatcactc
     1801 aatgcttgca taccgcgttc tcgatcggta ggatttttga aactaatttt taaagcaccg
     1861 tatatatctt cgcgtacttc taagattctt aagttgctta tagatatatt atgtaaactt
     1921 aggatataag tcactttgct tatcatacct gattcatccg gaatatctac atatagatca
     1981 tacgcagtat ttagtccacc tagttgttta gcgggcagtg cgtcacgata cgatttagct
     2041 tcggcaaaaa atgataacaa tttttcagaa tcattgcttt caattagtct ttctaaatct
     2101 tgaaactgac tttttagctg tcgaatcatt tctaaaatat acgttttatt actcaaggtg
     2161 atatctttcc acatttgtgc attactacta gctatacgag tgatatcacg aaaaccacca
     2221 gctgcaagtt tattgactaa atgatgttct tgactgttct tttgactaac atgaactaaa
     2281 ctagatgcaa cgatatgagg taaatgactt acgacgcttg ttacgtagtc gtgttcttca
     2341 gcagtagtta caataaattt agcaagagta ggtgataaca gttcttttaa cgtgtttgct
     2401 gcttgctcat ttcttggctc attgtagact aaaatataat aagcgttttc aaataagtgc
     2461 tttttagcat ttagtacacc ggatttatga ctaccagcca ttggatgacc actgactaaa
     2521 tgaatattat gctttaataa actgcattcg tgttgctgta tcattgcttt agtactacca
     2581 gtatcagaaa caataacacc aggtttagtt ggcatatgta taagctcgct aagatattta
     2641 tttgtgatag caacaggtgt tgcataaata attacatcgg cttttttaat agcttcacta
     2701 taatttaaac atttttcatt aataataccg attgatttag ctttatctaa ctgagaagta
     2761 tctgcatcgt atgcaataat attagtatta gggttatggt attttatatt gctagcaaga
     2821 cttccaccaa ttaatccaag cccaacaaat aaaactgttg tcatataaat caccttattt
     2881 cgaaattttc agaataataa cattgtaaat gagctgttga cacagtgcaa tagtaaataa
     2941 aaatcgataa tagcattaat agataaacgg atataaatca tctacaataa agagtatagt
     3001 aacacaatgg caacggaggg gtaaatcaat ggaaccaata ttagaaatga ttaaaacatt
     3061 aacaggtatt aatagtcctt caggagacac agaagaagca attcaatttg tcgaaaaata
     3121 tgcaaaagac ttgggttatc aaacaacact aacaaataaa ggtgcgttat taataacagt
     3181 gccaggcaaa aatgatgaag tacaacgctg tattactgct catgttgata ctttaggtgc
     3241 aatggttaaa gaaattaaag aagatgggcg cttagcaata gaattaattg gaggattcac
     3301 gtataacgcg attgagggtg aatattgcca aattaaaact gatgctggtc aaatatatac
     3361 aggaaccatt tgtctgcatg aaacaagtgt tcatgtatat agaaataatc atgaaatacc
     3421 tagagaccaa aagcatatgg aaataagaat tgatgaagta actacatcag aagaagatac
     3481 aaagagttta ggtatttcag taggtgattt tgttagcttt gatccacgta cagttatcac
     3541 gtcatcaggt tttattaaat ctcgtcattt agatgataaa gctagcgtag cgatgatact
     3601 aaaattacta aagaaattaa aagaagagca aataatatta ccacatacaa cgcaatttta
     3661 tatttctaat aacgaagaaa taggttacgg tgcaaatgca tcaattgatt cgaaaatcaa
     3721 agaatatatt gcattagata tgggcgcgtt gggagacggt caagcatccg atgaatatac
     3781 agtttctatt tgtgccaaag attcttcagg tccatatcat aagcaattga aatcacacct
     3841 agttaatctt tgcaaaataa ataatattcc atataaagta gacatatatc catattatgg
     3901 ttcagatgct tcagcagctt tacatgctgg tgcggatatc agacatggtt tatttggcgc
     3961 tggcattgaa tcatctcatg caatggaacg aacacatatt gattctatta aagcgacaga
     4021 gaaattacta tatgcatatt gcttatcacc aattgagtaa acaattagtg ttgacaaatg
     4081 tgaacgacct atgtaatata atgaactata aaaataatta gaattttcta aagaaatagt
     4141 agcagatatg aaacgtagca aatagaaagc taatgggtga tgggaattag cacgccatat
     4201 cttgtgaatt ggactttgga aaacaattga atgagttttg aaagtgaaca tgaattatgt
     4261 taactaaggt ggcaccacgg taacgcgtcc ttacaggtat atgcgttatg tggtgtcttt
     4321 ttatttagac aaaatgtagt agttagttaa aggtagcaac agaaagttag tgggtgatgt
     4381 gaactaacac cgagattaat gaaattgggt tttgtctgca acagaaaaat tatatatagt
     4441 aaagagtgaa ctatgaatat ttcgaatatt cggttaattt aggtggtacc acgcgtcagc
     4501 gtcctttata ttgataagga tgctgacgtt tttttagaaa ggagcgtaaa gaatggatgt
     4561 attctataaa aaaataaaag caaatgtaac acctgaagtt ttagcacaac ttcattccaa
     4621 gaagatcatt ttggaaagta caaatcaaca acaaactaag ggtcgctatt ctatagtgat
     4681 atttgatatc tacggtactt taactttaga taacgatgta ttatcagtaa gcactttaaa
     4741 agagtcgtat caaatcactg aaagaccgta tcattattta acgactaaaa taaatgaaga
     4801 ctaccataat attcaagatg agcaacttaa gccaataccg tttatatctg gatatgttgg
     4861 gacgtgtagc tttgatttag taagacatga atttcctaaa ttgcaatcaa tacaattaga
     4921 ggatcacaag caacacgatg tacggttata tatggttgaa caagtttatg tatttgacca
     4981 ttacaaagat gagttatata tcatcgcgac gaatcaattt tcaaattcaa cgaaatcaga
     5041 tcttgagaat cgagttaata agtctatcga agacttaact aaaatccaac cattcatgcc
     5101 tacacaagat tttgatttta aaactaaaga aattcaatca aatatttctg aagaaagatt
     5161 tatcgaaatg attcagtatt tcaaagagaa aataacagaa ggggatatgt tccaagttgt
     5221 gccatcaaga atttacaaat atgcgcatca tgctaatcag catttaaatc aacttacgtt
     5281 tcaactgtat caaaatttaa aacgacaaaa cccaagtcca tatatgtatt atcttaatat
     5341 cgatcaaccc tacattgtcg gtagttctcc cgaaagtttc gtaagtgtca aagatcaaat
     5401 tgtaacaact aatcctattg caggtacgat tcaacgtggt gagacgacac aaatagataa
     5461 tgagaatatg aaacaactac ttaatgatcc taaagaatgc agtgaacatc gtatgcttgt
     5521 tgatttaggg cgtaatgata ttcatagagt aagtaaaata ggtacctcaa aaattactaa
     5581 attaatggtt attgaaaaat atgaacatgt tatgcatatc gtaagtgaag tcacaggtga
     5641 aataaatcaa aatttatcgc caatgacagt tattgcgaat ttattaccaa caggtaccgt
     5701 ttcaggtgca ccaaaattac gtgcaatcga aagaatttac gaacaatatc cacataaacg
     5761 tggcgtttat agtggtggtg ttggatacat aaattgtaat cataacttag attttgcatt
     5821 agcaattcga acgatgatga tagatgagca gtatatcaac gtagaagctg gttgtggggt
     5881 tgtatatgat tctattcctg aaaaagaact aaatgaaacg aaattgaaag ccaaaagttt
     5941 attggaggta agcccatgat tttagttgta gataattatg attcctttac gtataaccta
     6001 gtggatattg tggcacaaca cactgatgtc attattcaat accctgatga tgataatgtg
     6061 ctgaatcaat ctgttgatgc tgtcattatt tcaccaggcc ctgggcatcc attagacgac
     6121 caacatttaa tgaaaattat ctcatcctat caagataaac ccattttagg tatttgccta
     6181 ggtgctcaag cactgacttg ttattacggt ggagaagtca ttaaaggcga caaagttatg
     6241 cacggaaaag ttgatacgct taaggttata tcgcatcatc aacatctgtt atatcaagat
     6301 gtaccagaac aattttctat tatgagatac cattcattaa taagtaatcc tgataatttt
     6361 ccagaagaat tgaaaattac tggacgtact gatgattgta tacaatcatt cgagcataaa
     6421 gaaagaccgc attatggtat tcagtaccat cctgaatcat ttgctacaga ctatggtgtc
     6481 aaaataatta caaattttat taattacgtg aagaaaggat gaaaaccatg acattactaa
     6541 caagaatcca aactgaatct atattgcttg aaagtgacat taaggagtta atcgacatac
     6601 ttatttcccc aagtatcgga actgatataa aatatgaatt actgagttcg tattcggaac
     6661 gagaaattca acaacaagaa ttgacatata ttgtacgtag tttaattaat acaatgtacc
     6721 cacatcagcc ttgttatgag ggcggtatgt gtgtgtggta caggtggtga caagtctaat
     6781 agcttcaata tatcaactac tgtcgctttt gttgtagcaa gtgctggcgt aaaagttata
     6841 aaacatggaa ataaaagtgt gacttcaaat tcaggtagta cggatttgtt aaatcaaatg
     6901 gacattcaaa ctacgtctgt tgatgatacg ccaaagcaat tagatgaaaa aaacctcgtg
     6961 tttatcggcg caacagaatc gtatccaata atgaaatata tgcaaccagt tagaaaaatg
     7021 ataggtaagc ctacaatatt aaaccttgta ggtccattaa ttaatcccta tcacctaacg
     7081 tatcaaatgg taggtatctt tgaccctaca aaactaaaac tagttgcaaa gacaattaaa
     7141 gatttaggta gacgacgtgc aatcgtatta catggtgcga atggtatgga tgaagcaacg
     7201 ttgtctggtg ataatttgat atatgaattg actgaagatg gagaaatcaa aaattacaca
     7261 ttaaatgcga ctgattatgg tttgaaatat gcacagaata gtgattttaa aggtggttcg
     7321 cctgaagaaa acttagcaat cacacttaat atcttgaatg gtaaagatca gtcaagtcgc
     7381 cgtgatgttg tcatacttaa tgcaggttta agtctatatg ttgcagagaa agtggatagc
     7441 atcgcagaag gcatagaact cgcaagcact ttgatagata atggtgaagc tttgaaaaaa
     7501 taccatcaaa tgagaggtga ataacatgac aattttagaa gaaattgttg aatataaaca
     7561 gttactttta caaaatggct attatcaaga caaacttaat accttgaaaa gtgtgaacat
     7621 tcagaataaa aaatctttta taaatgcaat tgagaaagaa ccaaagctag caattattgc
     7681 agaaattaaa tcgaagagtc ctacagttaa tgacttacct gaacgagatt tatcgcaaca
     7741 aatctcagat tatgagcaat atggtgcaaa tgctgtatcc attttgactg atgaaaagta
     7801 ctttggtggt agttttgaaa gattacaagc attgacaaca aaaacaacat tacccgtatt
     7861 atgcaaagat tttattatag atccgcttca aattgatgtt gccaaacaag ctggtgcatc
     7921 tatgatttta ctgattgtta atatcttatc ggataaacaa ttgaaagatt tatataacta
     7981 cgctatatcg caaaatttag aagtgttagt tgaagtacat gatcgtcatg aattagaacg
     8041 tgcctataag gttaatgcta aattgattgg tgtaaataac agggacttaa aacgatttgt
     8101 cacaaatgtg gaacatacaa atactatttt agaaaataaa aaaccaaatc attattatat
     8161 ttctgaaagt ggaattcacg atgtatctga tgtaagaaaa atcttgcata gtggtatcga
     8221 tggcttacta ataggtgagg cgcttatgcg ttgtgacaat ctatctgaat ttttaccaca
     8281 actgaaaatg caaaaggtga agtcatgatg aaattgaaat tttgtggctt tacatcaatt
     8341 aaggatgtta cagcggccag tcaattacct attgatgcga taggtttcat ccattatgaa
     8401 aaaagtaaaa ggcatcaaac aattacccaa ataaaaaagt tagcgtctgc tgttccagat
     8461 catattgaca aagtatgtgt cgtggtaaat cctgatttaa caactattga acacgtatta
     8521 agcaatacgc caattaacac aatacagtta cacggcacag aatctattga ttttatacag
     8581 gaaataaaaa agaagtattc aagcattaaa attactaaag ctttagctgc agatgaaaac
     8641 ataatccaaa acataaataa atataaaggg ttcgtagatt tatttattat cgacaccccc
     8701 tcagtgtcgt atggtggtac cggtcaaaca tatgactgga ctattttgaa gaacataaaa
     8761 gacatacctt acttgatagc aggaggcatt aacactgaaa atattcaaac agttaatcaa
     8821 tttaaattat cacatcaagg ttttgatctt gcatcaggta tagaagtaaa tgggcgaaaa
     8881 gatatagaaa aaatgacagc aattgtaaat attgtgaaag gagatagaga aaatgaataa
     8941 acaaatacaa acagaagcag atgaattagg tttctttggt gaatacggag ggcaatatgt
     9001 tccagaaaca ttaatgccag caattattga gttgaaaaaa gcttataaag aggcaaaagc
     9061 agacccagag tttcaaagag aacttgaata ctatttatca gagtatgtag gacgcgcgac
     9121 gccacttaca tatgctgcat cgtatactga aagcttaggt ggcgctaaga tatatttaaa
     9181 acgagaggat ctaaatcata caggcgccca taaaattaat aatgcgttag gtcaagcgtt
     9241 gctcgctaaa agaatgggca agaagaagct tgttgctgaa acaggtgcgg gtcaacatgg
     9301 tgtagctagt gctacggttg ctgcattatt tgatatggaa cttgttgtct ttatgggaag
     9361 tgaagatatt aaaagacaac aacttaatgt atttagaatg gaattacttg gtgcaaaggt
     9421 tgtggcagtt gaagatggtc aagggacttt atcggatgca gttaataaag cattgcaata
     9481 ttgggtaagt catgtagatg atacgcatta tttattaggt tctgcattgg gtccagaccc
     9541 attcccaacg attgttagag attttcagag tgtgattggt aaagaaataa aatcacagat
     9601 attgaagaaa gaaggtcgac ttccggatgc aattgtagca tgtatcggtg gtggctcaaa
     9661 tgcaatcggt acattttatc catttattaa agatgatgtt gctttatacg gtgttgaagc
     9721 cgcaggtcaa ggcgaggata ctgataaaca tgcacttgca attggcaaag gatcacctgg
     9781 cgtattacat ggtactaaaa tgtatttaat tcaagatgaa ggtgggcaag tgcaactagc
     9841 acattctatt tcagcaggac ttgattatcc tggtattggg ccagaacatt cttattacca
     9901 cgacattggt agagtaactt ttgaaaatgc aagtgataca caagcaatga atgctttaat
     9961 caactttaca aaacatgaag gtattatacc tgcaattgaa agtgcacatg cactgagtta
    10021 tgttgaaaga ctagcgccta cgatgtcgaa agaagatatt attgtagtaa caatttctgg
    10081 acgtggcgat aaagatatgg aaacaattag acaatatatg gcagagcgag gtcttgcaaa
    10141 tgactaaatt atttatacct tatattatgg gcaataaaga tttgattgaa aatgcaacat
    10201 tgttgagtga aaatggtgca gatataattg aaattggtgt acctttctct gatccggttg
    10261 ctgatggtcc agttatcatg gaagcaggtc aacaagcgat taaacaaggc ataacgatag
    10321 attatatttt cgagcaatta gaaaaacacg gtaatcaaat taagtgtcag tatgtattaa
    10381 tgacgacgta ttataatata atttgtcatt atggagaaca agcgtttttt gaaaaatgtc
    10441 gagataccgg tgtctacggc ttaattattc ctgatttacc atttgaatta tcgcagcgtt
    10501 taaaacaaca atttagtcac tatggtgtca aaatcatatc gttagttgcg atgactactg
    10561 atgacaaacg tataaaagat atcgtatcat atgcggaagg ctttatttat actgtgacaa
    10621 tgaatgcgac gacaggacaa aacggtgcgt ttcacccaga attaaaacga aaaattgagt
    10681 caattaaagc gatagccaat gtgccagttg tcgcaggatt tggtataaga tctccacaac
    10741 atgttgcaga tataaaagag gttgcagatg gtatcgttat tggtagtgaa atagtaaaac
    10801 gatttaaatc taacacgcgt gaagaaatca ttaaatattt acaatctatc cagcaaacat
    10861 tgaataatta agtttacttg atttaaaaaa attagtcgaa tactgtttga aaaagtgaaa
    10921 aacggtgaat tataaaattg aatagaaatg atacaaaagt aatatgagca aacccaaacg
    10981 ttcatattac tttttttgaa attgtatgca aaaatctaaa tattactata aaagtatacg
    11041 caattaaagc gtttatgttt tagttttaac attaactatt gtatacttat ttagattaga
    11101 tttattattt ttgacatttg cagaggggaa atagaaaaac tgcaaatacg gaaatgaaat
    11161 taattaacga gagacaaata ggagtaatga taatgaagtt tacaaattta acagctaaag
    11221 agtttggtgc ctttacagat agcatgccat acagtcattt cacgcaaact gttggccact
    11281 atgagttaaa gcttgctgaa ggttatgaaa cacatttagt gggaataaaa aacaataata
    11341 acgaggttat tgcagcttgc ttacttactg ctgtacctgt tatgaaagtg ttcaagtatt
    11401 tttattcaaa tcgcggtcca gtgatcgatt atgaaaatca agaactcgta cactttttct
    11461 ttaatgaatt atcaaaatat gttaaaaaac atcgttgtct atacctacat atcgatccat
    11521 atttaccata tcaatacttg aatcatgatg gcgagattac aggtaatgct ggtaatgatt
    11581 ggttctttga taaaatgagt aacttaggat ttgaacatac tggattccat aaaggatttg
    11641 atcctgtgct acaaattcgt tatcactcag tgttagattt aaaagataaa acagcagatg
    11701 acatcattaa aaatatggat ggacttagaa aaagaaacac gaaaaaagtt aaaaagaatg
    11761 gtgttaaagt aagatattta tctgaagaag aactaccaat ttttagatca tttatggaag
    11821 atacgtcaga atcaaaagct tttgctgatc gtgatgacaa gttttattac aatcgcttaa
    11881 aatattacaa agagcgtgtg ttagtgcctt tagcgtatat caattttgat gaatatatta
    11941 aagaactaaa tgaagagcgt gatattttaa ataaagattt aaataaagca ttaaaggata
    12001 ttgaaaaacg tcctgaaaat aaaaaagcac ataacaagcg agataactta caacaacaac
    12061 ttgatgcaaa tgagcaaaag attgaagaag gtaaacgtct tcaagaagaa catggtaatg
    12121 aattacctat ctctgctggt ttcttcttta tcaatccatt tgaagttgtt tattatgctg
    12181 gtggtacatc aaatgcattc cgtcattttg ccggaagtta tgcagtgcaa tgggaaatga
    12241 ttaattatgc attaaatcat ggcattgacc gttataattt ctatggtgtt agtggtaaat
    12301 ttacagaaga tgctgaagat gctggtgtag ttaaattcaa aaaaggttac aatgctgaaa
    12361 ttattgaata tgttggtgac tttattaaac caattaataa acctgtttac gcagcatata
    12421 ccgcacttaa aaaagttaaa gacagaattt tttaggaagg gaattatcaa aacatgaaat
    12481 ttacagagtt aactgttacc gaatttgaca actttgtaca aaatccatca ttggaaagtc
    12541 attatttcca agtaaaagaa aatatagtta cccgtgagaa tgatggcttt gaagtagttt
    12601 tattaggtat taaagacgac aataacaaag taattgcagc aagccttttc tctaaaattc
    12661 ctactatggg aagttatgtt tactattcga atcgtggtcc agtaatggat ttttcagatt
    12721 taggattagt tgattattat ttaaaagagt tagataaata tttacagcaa catcaatgtt
    12781 tatatgttaa attagatccg tattggttat atcatctata tgataaagat atcgtgccat
    12841 ttgaaggtcg cgagaaaaat gatgccttag taaacttgtt taaatcgcat ggttacgagc
    12901 atcatggctt tacaactgag tatgatacat cgagccaagt acgatggatg ggcgtattaa
    12961 accttgaagg taaaacacct gaaacattga aaaagacatt tgatagtcaa cgtaaacgta
    13021 atattaataa agcgataaac tatggtgtta aagtaagatt ccttgaacgt gatgagttca
    13081 atctattcct agatttatat cgtgaaactg aagagcgtgc tggatttgta tcaaaaacag
    13141 atgattattt ttataacttt attgacacat atggagataa agtattagta ccattagcat
    13201 atattgacct tgatgaatat gtgttaaagt tgcaacagga attgaatgac aaagaaaatc
    13261 gtcgtgatca aatgatggcg aaagaaaaca aatcagataa acaaatgaag aaaattgcag
    13321 aattagataa gcaaattgat catgatcagc atgaattatt gaatgcaagt gaattgagca
    13381 aaacggacgg cccaattcta aaccttgctt ctggcgttta ttttgcaaat gcatatgaag
    13441 tgaattattt ctctggtggt tcatcagaaa aatacaatca atttatggga ccttacatga
    13501 tgcattggtt tatgattaac tattgcttcg ataatggtta tgatcgttat aatttctatg
    13561 gtttatcagg tgattttacg gaaaacagtg aagattatgg cgtataccgc tttaaacgtg
    13621 gatttaatgt acaaatcgaa gaattaatag gggatttcta taaaccaatt cataaagtga
    13681 aatattggtt attcacaaca ttggataaat tacgtaaaaa attaaagaaa tagatgtaaa
    13741 tgatgttagg gcttatagtt attgatacta taggctcttt tttatacttt ttaataaagc
    13801 ctctaatgat ttattaaatg acaagtttaa caagtgattg caataaatat aaatacgaat
    13861 tgcacaatct attttcaaat gattaagttg agtatacgtt ttaattcatt caatatgact
    13921 caaaacaagc caatatgtat tatatttttg gtgaataaac cttagcataa caatttatat
    13981 ctgattttat atgtgtgttt gcaagtgcgt gatatcaaat gtcatatgta atttataaaa
    14041 tgcatgagaa atgattgctg taa
//
LOCUS       DAKSQE010000070        13810 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.070, whole genome shotgun sequence.
ACCESSION   DAKSQE010000070 DAKSQE010000000
VERSION     DAKSQE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.070"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            56..1021
                     /locus_tag="O0V33_001777"
     CDS             56..1021
                     /locus_tag="O0V33_001777"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCX8976403.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            1023..2372
                     /locus_tag="O0V33_001778"
     CDS             1023..2372
                     /locus_tag="O0V33_001778"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCX8976404.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDKNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            2388..3707
                     /locus_tag="O0V33_001779"
     CDS             2388..3707
                     /locus_tag="O0V33_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HCX8976405.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQADFDEEIYLINKDFKKEQGNEENNDSASSHANDNNIDDSTDSNIENEDYR
                     YNQETDDQNESNGIAVDNEQHQSAPNEQNSDSNDEETVTKKERKSKVTQLKPLTLEEK
                     RKVRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEEDPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLARDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSTQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            3813..5219
                     /gene="ftsA"
                     /locus_tag="O0V33_001780"
     CDS             3813..5219
                     /gene="ftsA"
                     /locus_tag="O0V33_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCX8976406.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDNEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESSDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKEESKFKKLMKSLFE"
     gene            5252..6424
                     /gene="ftsZ"
                     /locus_tag="O0V33_001781"
     CDS             5252..6424
                     /gene="ftsZ"
                     /locus_tag="O0V33_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCX8976407.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
     gene            6682..7473
                     /gene="pgeF"
                     /locus_tag="O0V33_001782"
     CDS             6682..7473
                     /gene="pgeF"
                     /locus_tag="O0V33_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HCX8976408.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDCPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAAL
                     LNNYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            7497..8165
                     /locus_tag="O0V33_001783"
     CDS             7497..8165
                     /locus_tag="O0V33_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCX8976409.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLEGFFQKKEALPSDAVIHFIGSLQSRKVKDVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            8162..8725
                     /locus_tag="O0V33_001784"
     CDS             8162..8725
                     /locus_tag="O0V33_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCX8976410.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTMSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            8737..9027
                     /locus_tag="O0V33_001785"
     CDS             8737..9027
                     /locus_tag="O0V33_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCX8976411.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYF
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIIVLVLFQKGLLQIFNWILIQLQ"
     gene            9136..9915
                     /locus_tag="O0V33_001786"
     CDS             9136..9915
                     /locus_tag="O0V33_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCX8976412.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFDLTLMEIDYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKIESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            9939..10535
                     /locus_tag="O0V33_001787"
     CDS             9939..10535
                     /locus_tag="O0V33_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCX8976413.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVASNTSDNNQQSNDSETTKK"
     gene            10756..13509
                     /gene="ileS"
                     /locus_tag="O0V33_001788"
     CDS             10756..13509
                     /gene="ileS"
                     /locus_tag="O0V33_001788"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCX8976414.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFNVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
     gene            complement(13620..>13810)
                     /locus_tag="O0V33_001789"
     CDS             complement(13620..>13810)
                     /locus_tag="O0V33_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCX8976415.1"
                     /translation="QRGTNENTNGLLREFFPKKTDLAKVNQEQLNYALDSINYRPRKC
                     LNWKFPYEVLCDELLHLN"
BASE COUNT         5041 a         1911 c         2643 g         4215 t
ORIGIN      
        1 aaattgataa tatattagtg gttaaaaaag tctggattaa gaagaggaga tagttatgat
       61 ttttgtatat gcgttattag cgctagtgat tacatttgtt ttggtacctg ttttaatacc
      121 tacattaaaa aggatgaaat ttggtcaaag tattcgagaa gaaggcccac aaagccatat
      181 gaagaagact ggtacaccaa cgatgggtgg actaacattt ctattaagta ttgtgataac
      241 gtctttggtg gctattatat ttgtagatca agctaatcca atcatactgt tattatttgt
      301 gacgattggt tttgggttaa ttggttttat agatgattat attattgttg ttaaaaagaa
      361 taaccaaggt ttaacaagta aacagaagtt tttggcgcaa attggtattg cgattatttt
      421 ctttgtttta agtaatgtat ttcatttggt gaatttttct acgagcatac atattccatt
      481 tacgaatgta gcaatcccac tatcatttgc atatgttatt ttcattgttt tttggcaagt
      541 aggtttttct aatgcggtaa atttaacaga tggtttagat ggattagcaa ctggactgtc
      601 aattatcgga tttacaatgt atgccatcat gagctttgtg ttaggagaaa cggcgattgg
      661 tattttctgt atcattatgt tgtttgcact tttaggattt ttaccatata acattaaccc
      721 tgctaaagtg tttatgggag atacaggtag cttagcttta ggtggtatat ttgctacgat
      781 ttcaatcatg cttaatcagg aattatcatt aatttttata ggtttagtat tcgtaattga
      841 aacattatct gttatgttac aagtcgctag ctttaaattg actggaaagc gtatatttaa
      901 aatgagtccg attcatcatc attttgaatt gataggttgg agtgaatgga aagtagttac
      961 agtattttgg gctgttggtc tgatttcagg tttaatcggt ttatggattg gagtgcatta
     1021 agatgcttaa ttacacaggg ttagaaaata aaaatgtatt agtcgtcggt ttggcaaaaa
     1081 gtggttatga agcagctaaa ttattaagta aattaggtgc gaatgtgact gtcaatgatg
     1141 ggaaagactt atcacaagat gctcatgcaa aagatttaga atctatgggc atttctgttg
     1201 taagtggaag tcatccatta acattacttg ataaaaatcc aataattgtt aaaaatcctg
     1261 gaataccata tacagtatct attattgatg aagcagtgaa acgaggtttg aaaattttaa
     1321 cagaagttga gttaagttat ctaatctctg aagcaccaat catagctgta acaggtacaa
     1381 atggtaaaac gacagttact tctctaattg gagatatgtt taaaaaaagt cgcttaactg
     1441 gaagattatc cggcaatatt ggttatgttg catctaaagt agcacaagaa gtaaagccta
     1501 cagattattt agttacagag ttgtcgtcat tccagttact tggaatcgaa aagtataaac
     1561 cacacattgc tataattact aacatttatt cagcgcatct agattaccat gaaaatttag
     1621 aaaactatca aaatgctaaa aagcaaatat ataaaaatca aacggaagaa gattatttga
     1681 tttgtaatta tcatcaaaga caagtgatag agtcggaaga attaaaagct aagacattgt
     1741 atttctcaac acaacaagaa gttgatggta tttatattaa agatggtttt atcgtttata
     1801 aaggtgttcg tattattaac actgaagatt tagtattgcc tggtgaacat aatttagaaa
     1861 atatattagc agctgtgctt gcttgtattt tagctggtgt acctattaaa gcaattattg
     1921 atagtttaac tacattttca ggaatagagc atagattgca atatgttggt actaatagaa
     1981 ctaataaata ttataatgat tccaaagcaa caaacacgct agcaacacag tttgccttaa
     2041 attcatttaa tcaaccaatc atttggttat gtggtggttt ggatcgaggg aatgaatttg
     2101 acgaactcat tccttatatg gaaaatgttc gcgcgatggt tgtattcgga caaacgaaag
     2161 ctaagtttgc taaactaggt aatagtcaag ggaaatcggt cattgaagcg aacaatgtcg
     2221 aagacgctgt tgataaagta caagatatta tagaacccaa tgatgttgta ttattgtcac
     2281 ctgcttgtgc aagttgggat caatatagta cttttgaaga gcgtggagag aaatttattg
     2341 aaagattccg tgcccattta ccatcttatt aaagggtgtg agtattgatg gatgataaaa
     2401 cgaagaacga tcaacaagaa tcaaatgaag ataaagatga attagaatta tttacgagga
     2461 atacatctaa gaaaagacgg caaagaaaaa gatcaaaggc tacacatttt tctaatcaaa
     2521 ataaagatga tacatctcaa caagctgatt ttgatgaaga aatttacttg ataaataaag
     2581 acttcaaaaa agaacaaggc aatgaagaaa ataatgattc tgcttctagt catgcgaatg
     2641 ataataatat cgatgattct acagactcta atattgaaaa tgaggattat agatataatc
     2701 aagaaactga cgaccaaaat gaatcgaatg gaattgcagt cgacaacgaa caacatcaat
     2761 cagctccaaa tgaacaaaat agcgactcga atgatgagga aacagtaacg aaaaaagaac
     2821 gaaaaagtaa agtaacacaa ttaaaaccat taacacttga agaaaagcgg aaggtaagac
     2881 gtaaacgaca aaaacgaatc caatacagtg ttattacaat attggtattg ttgattgctg
     2941 ttatattaat ttacatgttt tcaccactta gtaaaattgc gcatgtaaat ataaatggaa
     3001 ataatcacgt tagtacttca aagataaaca aagttttagg tgttaaaaat gattcgagga
     3061 tgtatacgtt tagtaaaaaa aatgctatta atgatctcga agaggatcca ttaatcaaaa
     3121 gtgttgagat acacaagcaa ttaccaaaca cattaaacgt agatatcaca gaaaatgaaa
     3181 ttattgcttt agtgaaatat aaaggtaaat atttaccttt attagaaaat ggtaaattgc
     3241 ttaaaggttc aaatgatgtc aaaattaatg atgcacctgt catggatggt ttcaaaggta
     3301 caaaagaaga tgatatgatt aaggcgttat ctgaaatgac acctgaagtt agacgatata
     3361 ttgccgaagt gacatacgcc ccaagtaaaa acaaacaaag cagaattgaa ttgtttacga
     3421 cggatggact tcaagtaatc ggtgatattt cgacgatatc taagaaaatg aaatattatc
     3481 cgcagatgtc acaatcatta gcaagagata gttcgggtaa actaaaaaca cgaggctata
     3541 ttgatttatc agtcggtgct tcatttatcc cataccgtgg aaacacgtct actcaatcag
     3601 aaagcgataa aaatgtgact aaatcatctc aagaggaaaa tcaagcaaaa gaagaattac
     3661 aaagcgtttt aaacaagatt aacaaacaat caagtaagaa taattaaaaa attttgatat
     3721 tgtctatgtt tatagttcac aagccattca acgtattgta aactaaggat agtgtatttt
     3781 tttaatagta atttgtcagg aggtgcctat ctatggaaga acattactac gtaagtattg
     3841 atattggatc atcaagcgta aaaacaatag taggcgagaa atttcacaat ggtataaatg
     3901 tgataggtac aggacaaacc tacacgagcg gtataaaaaa tggtttaatt gatgattttg
     3961 atattgcgcg acaagcaatc aaagacacaa ttaaaaaggc atcaatcgct tcgggtgttg
     4021 atattaaaga agttttcctg aaattaccta tcattggaac ggaagtttat gatgaatcaa
     4081 atgaaatcga cttttatgag gatacagaaa tcaacggttc acatatcgaa aaagtattag
     4141 aaggtattag agaaaaaaat gatgtgcaag aaacagaagt aattaatgta ttcccaattc
     4201 gttttatagt cgataaagaa aatgaggttt cagaccctaa agaattaatt gccagacatt
     4261 cattaaaggt tgaagcaggc gtaattgcta ttcaaaaatc gattttaatt aatatgatta
     4321 aatgcgttga agcatgtggt gttgatgtat tagatgttta ttctgatgcg tataactatg
     4381 gttcaatcct aacagctact gaaaaagagt taggtgcatg tgtcattgat attggtgaag
     4441 acgttacgca agttgctttt tatgaacgcg gtgaattagt agatgctgat tctatcgaaa
     4501 tggcagggcg tgatattaca gacgatattg cacaaggatt aaacacttct tatgaaactg
     4561 ctgaaaaagt taaacaccaa tacggtcatg cattctatga ttctgcttca gatcaagata
     4621 tcttcactgt tgaacaggtt gatagtgatg aaacagtaca gtatactcaa aaagatttga
     4681 gtgactttat tgaagcgcgt gtagaagaaa tattcttcga agtatttgat gttttacaag
     4741 atttaggatt aacaaaagta aatggtgggt ttattgtaac tggtggatct gcaaacttac
     4801 ttggcgtaaa agaattatta tcagatatgg taagtgaaaa agttagaatt cacacgccat
     4861 cacaaatggg aattagaaaa cctgaatttt cttcagcaat ttctacaatt tctagtagta
     4921 tcgcttttga tgagttatta gattatgtta caattaatta tcatgataat gaagaaactg
     4981 aagaagatgt tattgatgtg aaagacaaag ataacgaatc taaattaggc ggatttgatt
     5041 ggtttaaacg taaaacaaac aaaaaagata ctcatgaaaa tgaagtagag tcatcagatg
     5101 aagaaattta tcaatcagaa gataatcatc aggaacataa acagaatcat gaacatgttc
     5161 aagacaaaga taaagaagaa agtaaattca aaaaactaat gaaatctcta tttgaatgat
     5221 tattggccaa taaaactagg aggaaattta aatgttagaa tttgaacaag gatttaatca
     5281 tttagcgact ttaaaggtca ttggtgtagg tggtggcggt aacaacgccg taaaccgaat
     5341 gattgaccat ggaatgaata atgttgaatt tatcgctatc aacacagacg gtcaagcttt
     5401 aaacttatct aaagctgaat ctaaaatcca aatcggtgaa aaattaacac gaggtttagg
     5461 agcaggagct aaccctgaaa tcggtaaaaa agctgcagag gaatctcgtg aacaaattga
     5521 agatgcaatc caaggtgcag acatggtatt tgttacttct ggtatgggtg gcggaactgg
     5581 tactggtgca gcaccagtcg ttgctaaaat tgcaaaagaa atgggcgcat taactgttgg
     5641 tgttgtaact cgtccattta gttttgaagg acgtaaacgt caaactcaag ctgctgctgg
     5701 agtagaagct atgaaagctg cagtagatac attaatcgtt ataccaaatg accgtttatt
     5761 agatatcgtt gacaaatcta cgccaatgat ggaagcattt aaagaagctg ataacgtgtt
     5821 acgccaaggt gtacaaggta tctcagactt aatcgctgtt tctggtgaag taaacttaga
     5881 ctttgcagac gttaagacaa ttatgtctaa ccaaggttct gcattaatgg gtattggtgt
     5941 ttcttctggt gaaaatagag cggtagaagc tgctaaaaaa gcaatctctt ctccattact
     6001 tgaaacatct atcgttggtg cacaaggtgt gcttatgaat attactggtg gcgagtcatt
     6061 gtcattattt gaagcacaag aggctgctga tattgtccaa gatgctgcag atgaagacgt
     6121 taatatgatt ttcggtacag ttattaatcc tgaattacaa gatgagattg ttgtaacagt
     6181 tattgcaact ggttttgatg acaaaccaac atcacatggt cgtaaatctg gtagcactgg
     6241 attcggaaca agcgtaaata cttctagcaa tgcaacttct aaagatgaat cattcacttc
     6301 aaattcatca aatgcacaag caactgatag tgtaagtgaa agaacacata caactaaaga
     6361 agatgatatt cctagcttca ttagaaatag agaagaaaga cgttcaagaa gaacaagacg
     6421 ttaatcggtt aatatataca caaataattc aacacaaatc atcagataac atatctgatg
     6481 atttttttac taatttttag aacatgtaga aggacattta agtttttcga agtcattaaa
     6541 agtgtttaag tatcgtgtca aaattaagtc gaaaattatt tgcgcaacat tttaacttta
     6601 aacataaatg ttatattata taattattaa ctttgtacag ttagacgaag ataatttaaa
     6661 tgaaatgatg gtgacgatcg agtgaatgat aattttaaaa agcaaccgca tcatttaata
     6721 tatgaagaat tattacaaca aggtattact ctaggtatta caactagagg cgatggttta
     6781 agtgactatc ctaaaaatgc ttttaatatg gcgagatata ttgatgattg cccatataat
     6841 attactcaac atcaattgca attagctgaa gaaattgcgt ttgatagaaa aaattgggtg
     6901 tttcccatcc aaacacatga aaataaagtc gcttgtatta ccaaggatga tataggcaca
     6961 aatatagaca ctttaactga tgcgcttcat ggtattgatg cgatgtacac atatgatagt
     7021 aatgtcttat taacgatgtg ttatgcagac tgtgtaccag tatattttta tagtacaaaa
     7081 catcatttta ttgcattggc ccatgcaggg tggcgtggta cctatactga aattgtaaaa
     7141 gaagtgctaa aacatgtgaa ctttgatttg aaagacttac atgtcgttat tggaccatct
     7201 acatcatcaa gttatgaaat taatgatgat attaaaaata aatttgaaac attgccaatt
     7261 gatagtgcca actatattga aactagagga cgagatcgtc atggtattga tttgaaaaaa
     7321 gccaatgctg cattattaaa taattatggt gttcctaaag aaaatattta tacgacagcg
     7381 tatgctacat ctgaacattt agagttattt ttctcttatc gattagaaaa aggtcaaaca
     7441 ggacgcatgt tagcattcat tggtcaacag taaacaagga ggagatatgt ttgcgtgtga
     7501 aagataattt acaacaaatc tcaacacaaa ttaatgacaa aagtgaaaaa aataattttt
     7561 caacaaaacc aaacgtgatt gcagttacaa aatatgttac aatagagcga gctaaagaag
     7621 cgtatgaggc tggaataaga cattttggtg agaatagatt ggaaggcttt tttcaaaaga
     7681 aagaagcatt accatcagat gcggtgatcc attttatagg atcattacaa tctcgaaaag
     7741 ttaaggacgt tataaacgac gtagattatt tccatgcttt agatcgattg agcttagcca
     7801 aagaaattaa caaacgtgca gaacataaaa ttaaatgttt cttgcaagtg aacgtttcgg
     7861 gagaagcttc taagcatggt attgctttag aagatgttga tcagtttata gatgatctta
     7921 aaaaatatga caaaatcgaa attgtaggtt taatgacgat ggcaccattg acagatgatg
     7981 aagcatatat tagatcgtta tttaaacagt tacgtttgaa aaaagaagaa atacaacgac
     8041 tcaatttaga atatgcgcct tgtgatgaat tatcaatggg aatgagtaat gactatctta
     8101 ttgcagttga agaaggtgca acgtttgtta gaattgggac taaacttgta ggagaagagg
     8161 agtgagccac ttggctttaa aagatttatt tagtggattt tttgtaatag atgatgaaga
     8221 ggaagtagaa gtacctgaca aacaacaaca ggtaaatgaa gcgccagcaa aagagcagtc
     8281 acaacaaaca acaaaacaaa acgcaatcaa atcagtccct caaaaatctg catcaagata
     8341 tacaacaatg tcagaagaaa ggaataaccg tatgtctaat tattcaaaaa ataattcacg
     8401 taatgttgta actatgaaca atgctacacc aaacaatgca tcacaagaaa gttcaaaaat
     8461 gtgtttattc gaaccacgtg ttttttcaga tacacaagat attgctgatg agcttaaaaa
     8521 ccgccgtgcg acacttgtca atttacaacg tattgataaa gtatcagcga aaagaattat
     8581 tgatttttta agcggtactg tttatgcaat cggtggagat atccaacgtg taggtactga
     8641 tattttctta tgtacgcctg ataatgtgga agtagctgga agcattacag accatattga
     8701 aaatatggaa cattcattcg actaaggagt taacatatgg atataaatgt gctagctaca
     8761 atttttaaat ttatcctttt tgttgttgaa atttattatt tcggcatgat tatatatttc
     8821 tttacatctt gggtaccaag tattagagaa actaaggtag gttatttttt agcgaaaata
     8881 tatgaacctt tcttacaacc atttagaaaa gtaattccac ctattggaat tatcgacata
     8941 tcatcaatcg ctgcaattat cgttttagta ttattccaaa aagggttact ccaaatcttt
     9001 aattggattt taattcaatt acaataaatt aagcgacata acgaggcaag ggtacatgat
     9061 actttagcct cgtttttgat atgtattttt ctaaatataa gggcaataga tggtatttta
     9121 taatttttta aggtagtgat taacatagat atttatcaac actttagaca ggaagaatac
     9181 gaattaattg atcagctaac ggataaatgt gatcaagcgg aacagcatta tgcaccagta
     9241 ttaacgcatt ttttagatcc aagagggcaa tatatattgg aagtgatttg tggcagttat
     9301 gaagatttaa acgtatcttt ttatggcggg cctaatgctg aaagaaaaag agcaatcatt
     9361 tcgccgaact attacgaacc taaagaaagt gactttgatt taactttaat ggaaatagat
     9421 tatcctgaaa aattcgtcac tttaaaacat caacatattt tagggacatt aatgtcttta
     9481 ggtatcgaac gcgaacaagt tggagatata attgtgaatg aacgaattca atttgttttg
     9541 acaagtagat tggaatcatt tattatgtta gaattacaac gtattaaagg cgcatcagtt
     9601 aaactttata ctattccagt aacagatatg atacaatcta atgagaattg gaaaattgaa
     9661 agtgcaacag ttagttcttt aaggttagat gttgttatta aagaaatgat acgtaaatca
     9721 cgtacgattg cgaaacaact aatcgaaaaa aaacgtgtta aagtgaatca cactattgtt
     9781 gattcagcag attttcaatt acaagcaaat gatttaatat ccatccaagg ttttggtaga
     9841 gcacacatta ctgacttagg tggtaaaact aaaaaagata aaacgcacat tacctataga
     9901 acattattca aatagtaatg atttaaggag gataacaaat gccttttaca ccaaatgaaa
     9961 ttaagaataa agagttttca cgtgtaaaga atggtttaga acctactgaa gttgctaatt
    10021 ttttggagca actaagcact gaaattgaac gtcttaaaga agataaaaaa caacttgaaa
    10081 aagtaatcga agagagagat actaatatta agtcttatca agacgtgcat caatctgtaa
    10141 gtgatgcttt gatacaagct caaaaagctg gtgaagaaac taagcaagct gcagagaaac
    10201 aagctgaagc gattatagct aaggcagaag cacaagctaa tcaaatggtt ggtgacgcgg
    10261 tagaaaaagc acgccgttta gcattccaga ctgaagatat gaaacgtcaa tcaaaagtat
    10321 ttagatcgcg tttccgtatg ttagttgaag cgcaattaga cttattaaaa aacgaagatt
    10381 gggattactt gttgaattat gatttagacg ctgaacaagt gacgcttgaa aatattcatc
    10441 atttgcatga aaatgattta aagccagatg aagttgcatc aaatacatca gacaataatc
    10501 aacaatccaa tgattcagaa acaactaaga agtaagaatt aaataaagac agacgcgtaa
    10561 tatacattta acttttcaca gcgaattagg taatggtgag agcctagtaa aagcatgtat
    10621 gttatatcac tggcttttta atatttaaat aatgtaatga gagaactcta agttgagtta
    10681 ataagggtgg taccgcgagc aatcgtccct tttaatttaa cttagagttt tttaaatttt
    10741 taaggagtga aaaaaatgga ttacaaagaa acgttattaa tgcctaaaac agatttccca
    10801 atgcgaggtg gtttaccaaa caaggaaccg caaattcaag aaaaatggga tgcagaagat
    10861 caataccata aagcgttaga aaaaaataaa ggtaacgaaa cattcatttt acatgatggc
    10921 ccaccatacg cgaatggtaa cttacatatg ggacatgcct tgaacaaaat tttaaaagac
    10981 tttattgtac gttataaaac tatgcaaggg ttctatgcac catacgtacc aggttgggat
    11041 acacatggtt tgccaattga acaagcatta acgaaaaaag gtgttgaccg taagaaaatg
    11101 tcaacagctg aattccgtga gaaatgtaaa gaatttgctt tagaacaaat tgaattacag
    11161 aaaaaagatt ttagacgttt aggtgttcgt ggtgacttca atgatccata tattacatta
    11221 aaacctgaat acgaagctgc acaaattcgt atttttggag aaatggcaga taaaggttta
    11281 atttataaag gtaaaaagcc agtttattgg tctccttcaa gtgagtcttc attagcagaa
    11341 gcagaaattg aatatcacga taaacgttca gcatcaattt acgttgcatt taacgttaaa
    11401 gatgacaaag gtgtcgttga tgcagatgct aaatttatta tctggacaac aacgccatgg
    11461 acaattccat caaatgttgc gattaccgtt catccagaat taaaatacgg tcaatacaat
    11521 gtaaatggcg aaaaatatat tattgcagaa gccttatctg acgccgtagc agaagcactg
    11581 gattgggata aagcatcaat caaattagaa aaagaataca caggtaagga attggagtat
    11641 gttgtagcac aacatccatt cttagataga gaatcgttag tgattaatgg tgatcatgtt
    11701 actacagatg ctggtacagg ttgtgtacat acagcaccag gtcacgggga agatgactat
    11761 attgttggtc aaaaatatga attgccagta attagtccaa tcgatgataa aggtgtattt
    11821 actgaagaag gcggccaatt tgaaggaatg ttctatgata aagctaataa agccgttact
    11881 gatttattaa cagaaaaagg tgcactatta aaattagact ttattacaca tagctatcca
    11941 cacgactgga gaacaaaaaa acctgtaatt ttccgtgcta caccacaatg gtttgcttca
    12001 atcagtaaag taagacaaga tattttagat gcaatcgaaa atacaaactt caaagtaaat
    12061 tggggtaaaa cacgtattta caatatggtt cgtgaccgtg gcgaatgggt tatttctcgt
    12121 caacgtgttt ggggtgtacc gttaccagta ttttatgctg aaaatggcga aattatcatg
    12181 acgaaagaaa cagtgaatca tgttgctgat ttatttgcag aacacggttc aaatatttgg
    12241 tttgaaagag aagcgaaaga cttactacca gaaggattta cacatccagg cagccctaac
    12301 ggtacattta ctaaagaaac agacattatg gacgtttggt ttgattctgg ttcatcacac
    12361 cgtggcgtgt tggaaacaag accggaatta agtttcccag cagatatgta tttagaaggt
    12421 agtgaccaat atcgtggttg gttcaactct tctattacaa cttcagttgc tacaagagga
    12481 gtatcacctt ataaattctt actttctcat ggttttgtta tggacggtga aggtaagaaa
    12541 atgagtaaat ctttaggtaa tgtgattgta cctgaccaag tggttaaaca aaaaggtgct
    12601 gatattgcga gactttgggt aagtagtacg gactatttag ctgatgttag aatttctgat
    12661 gaaattttaa aacaaacatc tgatgtttat cgtaaaatca gaaatacatt aagatttatg
    12721 ttaggtaata ttaatgattt caatcctgat acagatagca ttcctgaatc agagttatta
    12781 gaagttgatc gttacttgct aaatcgttta cgtgaattta ctgcaagtac gattaacaac
    12841 tatgaaaact ttgactactt aaatatttat caagaagttc aaaactttat caatgttgag
    12901 ttaagtaatt tctatttgga ttacggtaaa gatattttat atattgaaca acgtgattct
    12961 catatccgtc gtagtatgca aacagtgtta tatcaaattt tagttgatat gacgaagttg
    13021 ttagcaccaa tcttagtgca tacagctgaa gaagtttggt ctcatacacc acatgttaaa
    13081 gaagaaagtg ttcacttagc agacatgcct aaagttgtag aagtagatca agctttattg
    13141 gataaatggc gtacatttat gaatttacgt gatgatgtga accgtgcatt agaaactgct
    13201 cgtaatgaaa aagttattgg taaatcatta gaagctaaag ttacgattgc tagtaacgat
    13261 aaatttaatg catctgaatt cttaacttca tttgatgcat tacatcaatt atttatcgtg
    13321 tcacaagtta aagttgtaga taagttagat gatcaggcaa cagcttatga acatggtgat
    13381 attgtcatcg aacatgcaga tggtgaaaaa tgtgaaagat gttggaacta ttcagaggat
    13441 cttggtgctg ttgatgaatt gacgcatcta tgcccacgat gccaacaagt tgtaaaatca
    13501 cttgtataat tgaaattgta taaagtactt atacagatga tataaattaa agctctcttc
    13561 ataatcatgt tgtagttttt gttgacatga tgaagagagt ttttttgaga gttgatttgt
    13621 caatttaagt gcaacagttc atcacataaa acttcataag gaaacttcca atttaaacac
    13681 tttcttggtc gataattaat tgagtctaat gcatagttaa gttgctcttg atttacttta
    13741 gctaagtctg ttttcttagg gaaaaattct cttagtaaac cattggtatt ttcatttgta
    13801 cctctttgcc
//
LOCUS       DAKSQE010000071        13141 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.071, whole genome shotgun sequence.
ACCESSION   DAKSQE010000071 DAKSQE010000000
VERSION     DAKSQE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13141
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.071"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            36..806
                     /locus_tag="O0V33_001790"
     CDS             36..806
                     /locus_tag="O0V33_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCX8976416.1"
                     /translation="MMKRLNKLMLGISFLFLVISITAGCGMGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIINSEMATQNKGEALKIKGMVLYMNRNTKTT
                     KGYYYVNAIKNDKDGRPQENEKRYPVKMVDNKVIPTKEIKDENIKAEIKNFKFFVQYG
                     NFKDLKNYKDGDISYNPESPIYSAKYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGSG
                     NLKGSSVGYKNIEFTFVEEKGKNIYFSDSLDYKKSGEV"
     gene            809..2185
                     /locus_tag="O0V33_001791"
     CDS             809..2185
                     /locus_tag="O0V33_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976417.1"
                     /translation="MAKAEYEIDPGKITSNSEETSAISNISYEIENANDNNLDNEKIR
                     GQITKLKKGGHFPKNLDYIESYTDPSTGTTATAFLNKDTGKVTVGMAGTNFHGDQIKK
                     VALSSMSPLLFPPSKQDMKDVRGTMKDGAADLAIGVGLVNYKGKHFANTQQFIENLQK
                     KYEIDTVTGHSLGGRDAIFLGLRYNIKNVVAYNPAPLEVKSIRNKLGGQLFRNTTFPD
                     EKYLKELMDNYDGDITKVITQKDGLDYLVKHTDHLTCGDVLRINNGQGHAMENFLGEK
                     EQREIIEELMLVKGYRDANDKAFKALKKNTEKKLGKIDEIKSKLLQTNGGALSSSQQK
                     LLETLVAFSVAEGLSKMVDQELQQLKNMFNLMDEKFEANWKDAQEASDIVGKHLSYPE
                     KVSALDNGGVNESKLATEPHNEIKEKLNKITDLSKKYNSYLKQIEKSINEIVAKDQQL
                     AGQIGDLI"
     gene            2185..2511
                     /locus_tag="O0V33_001792"
     CDS             2185..2511
                     /locus_tag="O0V33_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976418.1"
                     /translation="MFEEYKKIDDLENAYEIELKRIDREIQNLNDLKYHLRRENEQSY
                     DAFLYLKNKMNYSEESNAKVRRLVEEFDYEADSYIRKKELKLEDYKEEIRREYIQQSE
                     KIMEAK"
     gene            2515..2826
                     /locus_tag="O0V33_001793"
     CDS             2515..2826
                     /locus_tag="O0V33_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976419.1"
                     /translation="MGIPDPLSNPMSNPLLGDSWMVGDLKKRQEKKLITTVIAPEHDE
                     KHRENKSKMKKSEKQLINQLINHGHTFKNDFKNVAKGDWVNKAMQDLDKLGNELKKIM
                     N"
     gene            3075..5312
                     /locus_tag="O0V33_001794"
     CDS             3075..5312
                     /locus_tag="O0V33_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCX8976420.1"
                     /translation="MQRDYLIRVETESMSDFKRLNGLMIGFVIKGEAHIYDENNMTQC
                     NIGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDVHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFAESLGVGFQHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSVQHNYHDREL
                     ILLLNDYIEEMNHFIEDIEKVNYKEITFQPTNHQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHHDFRVNEVLAYDVMPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLDQYDSRDYIDAFKIFIHHVALHVSHRKDLKFNLYVTTLHTSLIEMIDYFKALFPN
                     GGLYIHLDQATERQLPLLKRLEPHIDHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYLIDLMHRHHLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSSKVEGIGY
                     WLNYDLHVSHCRNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEEGMYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDMTDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            5463..6641
                     /locus_tag="O0V33_001795"
     CDS             5463..6641
                     /locus_tag="O0V33_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCX8976421.1"
                     /translation="MNQQLIEALKSKEDKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKAMIENGVLDGVDHV
                     LGVHVMSTMKTGNVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEIKR
                     LSKGLEALYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEADLEFGVEICEPQPPSED
                     FAYYAKERPSAFIYTGAAVEDGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            6643..8031
                     /locus_tag="O0V33_001796"
     CDS             6643..8031
                     /locus_tag="O0V33_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCX8976422.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGADM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRIIQGVSAACIMPSTLAIMKTYYEGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEVTNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSFWFFSLIAIVIVAFFMFLKVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVIIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYAGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
     gene            complement(8519..10180)
                     /locus_tag="O0V33_001797"
     CDS             complement(8519..10180)
                     /locus_tag="O0V33_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCX8976423.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSTNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDVWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
     gene            complement(10505..12280)
                     /locus_tag="O0V33_001798"
     CDS             complement(10505..12280)
                     /locus_tag="O0V33_001798"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCX8976424.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DHASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHIGGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKVDVTTSQKIAREILIDRNGNAE
                     SIKLTINDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPKKSWFVSATSTTNNKDIIDTIESICKRDPLAGKTVTGGIITINDSAWQISFT
                     INRQQQFKNQPKNEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVPTDKI
                     EDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSTFDLRVLMDAVYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIESLLKEHRLL"
     gene            12632..13105
                     /locus_tag="O0V33_001799"
     CDS             12632..13105
                     /locus_tag="O0V33_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HCX8976425.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYSKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQFNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
BASE COUNT         4580 a         2019 c         2374 g         4168 t
ORIGIN      
        1 aaagtaaatc attttaaaaa gaagagagtt gtaagatgat gaaacgatta aataaattaa
       61 tgctaggtat tagttttctg tttttagtca ttagtatcac tgctggttgt ggtatgggta
      121 aagaagcgga aattaagaaa agttttgaaa agacattgag tatgtatcca attaaaaatc
      181 tagaggattt atacgataaa gaaggttatc gtgatgatca atttgataaa aatgataagg
      241 gaacatggat tattaattct gaaatggcga ctcaaaataa gggagaagct ttgaaaatta
      301 aaggtatggt cttatacatg aatagaaata caaaaacaac aaaaggatat tattatgtaa
      361 atgcaataaa gaatgataaa gacggaagac cccaagagaa tgaaaaaagg tacccagtta
      421 aaatggttga taataaagtt attccaacaa aagaaattaa agatgaaaac ataaaagcag
      481 aaatcaaaaa ctttaagttc tttgttcaat atggcaactt taaagatttg aaaaattata
      541 aagacggaga tatttcatat aatccagaat ccccgattta ttcagcgaaa tatcaactaa
      601 ctaatgatga ttataatgta aaacaattac gtaaaagata tgatatacca acaaataaag
      661 cgccaaagtt attgttaaaa gggtcaggga acttaaaagg ttcatcagtt ggatataaaa
      721 atatagaatt tacttttgta gaggaaaaag gtaaaaatat atactttagt gatagcttag
      781 attataaaaa aagtggagaa gtataattat ggctaaagca gaatatgaaa ttgatcctgg
      841 aaaaataaca agtaattcgg aagaaactag cgcaatatct aatattagtt atgaaataga
      901 aaatgcaaat gataataatt tagataatga aaaaattaga gggcaaatta ctaaattaaa
      961 aaagggcggg cattttccta aaaacttaga ctacatcgaa agttacacag atcccagtac
     1021 ggggaccacg gcaacggcat ttttaaataa agatacaggt aaagttactg taggaatggc
     1081 tgggacaaac tttcacggcg atcaaattaa aaaagtagca cttagttcga tgtctccgtt
     1141 attattccca ccctctaagc aagatatgaa agatgtacga ggcactatga aggatggtgc
     1201 tgcggatctt gctattggag ttggattggt caattataaa ggaaaacatt ttgcaaatac
     1261 acagcagttt attgagaatt tacaaaaaaa gtacgaaatt gataccgtta ctggtcattc
     1321 tttaggaggg agagatgcta tctttcttgg gcttcgttat aacatcaaaa atgttgtggc
     1381 atacaatcct gctccgttag aagtaaaaag cattcgtaat aaacttggtg gtcaattgtt
     1441 tagaaatacg acatttccag atgaaaaata tttaaaagag ttaatggata attatgatgg
     1501 agatattact aaggttataa ctcaaaagga tggattagac tatttagtga agcatacaga
     1561 tcatttaact tgtggtgatg tgttacgtat aaacaatggt cagggccatg cgatggagaa
     1621 ttttttagga gaaaaagaac aacgtgaaat aatcgaagaa ctaatgttag taaaaggata
     1681 tcgcgatgca aatgacaaag catttaaggc tttgaaaaaa aatactgaga aaaaactcgg
     1741 taaaatagat gaaatcaaat cgaagttact gcaaacgaac ggtggtgccc tctcgtcttc
     1801 tcaacaaaaa ttgcttgaaa ctttggtggc attttcggta gctgaaggac taagtaaaat
     1861 ggttgaccaa gaacttcagc agttgaaaaa tatgttcaat cttatggatg agaaatttga
     1921 agcaaattgg aaagacgcac aagaagctag tgatattgtg ggtaaacatc tttcataccc
     1981 agaaaaggta agtgcattag ataacggtgg cgtcaatgaa agtaaacttg cgaccgaacc
     2041 acataatgaa ataaaagaaa aactaaataa aattacggac ttatctaaga aatataattc
     2101 gtatttaaag caaatcgaaa aaagtattaa tgaaatagtt gctaaggatc aacaattggc
     2161 agggcaaata ggtgatttaa tatgatgttt gaagaataca aaaaaataga tgatcttgaa
     2221 aatgcatatg aaatagaatt gaaacgtatt gatagggaaa tacaaaatct taatgattta
     2281 aaatatcacc ttagaagaga aaatgaacaa agttatgatg catttttgta tttaaagaat
     2341 aaaatgaact atagtgaaga gtcaaatgct aaggtgagac gactagttga agaatttgat
     2401 tatgaagcag atagttatat tagaaaaaaa gagttaaagt tagaagatta taaagaagaa
     2461 ataagaagag aatatattca gcaatctgaa aaaataatgg aggcgaagta gcttatggga
     2521 atccctgatc cgttatctaa tcctatgtct aatcctttac taggagatag ttggatggta
     2581 ggtgatttga aaaaaagaca ggaaaaaaaa ctaatcacaa cagtcatcgc acctgaacat
     2641 gatgaaaagc atcgtgaaaa taaaagtaaa atgaaaaaat ctgaaaagca attgatcaat
     2701 cagttaatta atcatggcca tacatttaag aatgatttta aaaatgtagc taaaggtgat
     2761 tgggttaata aagccatgca agacttagat aaattaggta atgaacttaa aaaaataatg
     2821 aattagttga attgtcacat actttttcaa accaacaacg aactaaaaaa tctatataaa
     2881 tatgattaaa agaagtcata accattcgta aaggatggtt atttttaata gtaaacaaaa
     2941 ttgctgctaa cttacttttt aaaccgagtg aggataaata atcatccaca cacacgattc
     3001 aatatgaaat tcaaatatgt tgctgtaaaa taatgtaaaa taaacgtatt catattactg
     3061 aattgaggga tagtatgcaa cgtgattatt taattcgagt agaaactgag agtatgtcag
     3121 atttcaaaag gctcaatggt ttaatgattg gttttgttat taaaggtgag gcgcacattt
     3181 atgatgaaaa taatatgacg caatgcaaca ttggcgacat tttcatcatt aaccaccgcg
     3241 acttgtatcg atttcaactt caacaagatg gcatcatatg ttatatccaa ttccaaatga
     3301 aatatttagc agacaagttt gatgatgtgc attgtctata ttttcactta acagatgcga
     3361 ccacaacaaa aaatatacat caactgagaa atataatggc aagactggtt tcaacacaca
     3421 ttcgacataa tgagttgtct aaattgactg agcaacaact tgtgattcag ttgcttatgc
     3481 atatgattca ttatgtccca cgtacatatc attcgaacca aagtatctta aatgatgata
     3541 aagtgaatca agtatgcgac tatatcgagt tacattttca tgaagactta agcctttcgg
     3601 aattaagcga atatgttggg tggtcagaga gccatctgtc taaaaagttt gcagaatcgc
     3661 taggtgtagg attccagcat ttcttaaata cgacgcgaat tgagcatgcg aaactcgatt
     3721 tgacatacac agatgaaacg attactgata tagcattgca aaatggcttt tcaagtgcag
     3781 cgagctttgc gagaacattt aaacacttta cgcatcaaac gcctaaacaa tatcgaggtg
     3841 atcgtccggc aatcactgaa aatcaacaat cggtacaaca taattatcac gaccgtgaat
     3901 tgatattact tttaaatgac tacattgaag aaatgaatca tttcattgaa gatattgaaa
     3961 aggtgaacta taaagagatt acctttcaac caaccaatca ccaactaaat caatttaatc
     4021 atattattca agtgggctat ttgaggaatt tgctcaatac acagtatcaa tcacagttgc
     4081 ttacatgtca tcatgatttc cgtgtcaatg aagtattagc atatgatgtg atgccatata
     4141 tcatgaaaaa gctcaatgcg ccattcacgt atgatgcaga aatttcgaat atattttatg
     4201 atatcgattt gtgtttagac tttttattag atcataactt tagtttgacc atgcatttgg
     4261 atcagtatga ctcacgtgat tatatcgatg catttaaaat ttttatccat cacgttgccc
     4321 tgcatgtcag tcatagaaaa gatttgaagt tcaacttgta tgtgacgaca ttgcacacgt
     4381 ctttgattga aatgattgat tattttaaag cattattccc taatggtggc ttgtacattc
     4441 acttagatca agctacggaa agacagctac cattgttgaa acgacttgag ccacacatcg
     4501 accattttgt atttgatgcc aattcaaatg atgctgttga ttttaataaa atgaatgatg
     4561 atgaatttaa aaccgcaagt caaatgatta ttaataaaac gaattacctt atcgatttaa
     4621 tgcatcgtca tcacctaaag cgtccactca ttttactcaa ttggaataca ttgacgggtg
     4681 atacatttat tacaaacggc gaatatttta gaggtggtat catcattgag caattattaa
     4741 aattaagttc taaagtagag ggtatcgggt attggttgaa ttatgatttg cacgtcagtc
     4801 attgtagaaa tgaacgcgat tatatgaatt caattgaact gtttcatcaa tataatggaa
     4861 aacgtccggt ctatttcacg gcattgctat ttaataaatt aacaagcaat attttgtatt
     4921 ctgatgatac ttgtattgtc acgggaactg attcaaattt tcaaatattg ttatatgatg
     4981 caaagcattt taatccgtac ttagcgttgg acaatcaaat gaatatgcgt gcaacggaaa
     5041 tgatccattt gaacattaat gccctggaag aaggtatgta taagattaaa cattttacct
     5101 tagataaaga aaatggtgca ttatttaatc tttggcgcaa acatcatacg atacatggta
     5161 tggacaagga ttctatagat tacgttaatc gaatgagttt tccaaaatta gaagtgtacg
     5221 atatagatat gacggatacg ctggcattaa atattaaaat gattacaaat ggaattcact
     5281 taattgaagt aaaacgttac ccaagttcat aaaatgatca caaatcacaa tttatgttat
     5341 acataatttg tgattttttg tattcaaagt taaaattgca aaaaattaat ggttaacatc
     5401 tctgttgttg gcaatataaa taatgatcaa tcatatatga tgtaactaag gagatgaatg
     5461 atatgaatca acaattaatt gaagctttaa aatcaaaaga agacaaaatg attgagatca
     5521 gacgttattt acatcagcat ccagaattat cttttcatga ggatgaaacg gcgaaataca
     5581 tcgctgaatt ttacaaaggt aaagatgtag aagtagaaac gaatgtcgga ccacgtggaa
     5641 ttaaagtaac gattgattca gggaaacctg gtaaaacatt agcaatccgt gcagactttg
     5701 atgcattacc cattactgaa gatacaggat tatcttttgc atcacaaaat aaaggtgtta
     5761 tgcacgcatg tggtcacgat gcacatacag catacatgct tgtattagca gagacgcttg
     5821 ctgaaatgaa agatagtttt acaggaaaag tcgttgtgat acatcaacca gctgaagaag
     5881 taccaccagg tggtgctaaa gcaatgattg aaaatggtgt attagacggt gttgatcatg
     5941 tattaggtgt acacgtcatg agcacaatga aaacaggtaa tgtgtattac agacctggtt
     6001 atgttcaaac aggacgcgca ttcttcaaat tgaaagttca aggtaaaggt ggtcatggtt
     6061 catcaccaca tatggccaat gatgccattg ttgcaggtag ctacttcgtc acagcgttac
     6121 aaacagttgt atctagacga ctaagtccat ttgaaaccgg tgttgtcaca atcggttcat
     6181 ttgacggtaa aggtcaattc aatgtcatta aagatgttgt tgaaattgaa ggtgatgtac
     6241 gtggattaac agatgctaca aaagcaacaa ttgaaaaaga aattaaacgt ttatcaaaag
     6301 gattagaagc attgtatggt gtaacttgca cattagaata taacgatgat tatcctgcat
     6361 tatataatga tccagagttt actgagtacg tggctaagac gctaaaagaa gcagatcttg
     6421 aatttggtgt cgaaatatgt gaaccacaac caccttcaga agactttgca tactatgcta
     6481 aagaacgtcc aagtgccttt atttatacag gtgcagctgt ggaagatggt gaaatttacc
     6541 cacatcatca tcctaaattt aacatttcag aaaaatcatt acttatttcg gcagaagctg
     6601 tagggacagt tgttttagat taccttaaag gagataacta acatgaatga aacgtatcgc
     6661 gggggcaaca agttaatctt aggtattgta ttaggtgtta ttacattttg gttgtttgca
     6721 caatcacttg taaatgttgt accaaattta caacaaagtt ttggtgcaga catgggaaca
     6781 attagtattg cggtaagtct aaccgcacta ttttcaggca tgtttgttgt tggagcaggc
     6841 ggcctagcag ataaaattgg acgcgtgaaa atgacaaata tcggcttatt attaagtatt
     6901 attggttcag cattaattat tattacaaat ttaccagcat tgctaatttt aggtcgtatt
     6961 atacaaggcg tatcagcagc gtgtattatg ccttccacat tggccattat gaaaacttat
     7021 tatgagggtg ctgaacgtca gcgtgcctta agctactggt ctatcggttc ttggggtggc
     7081 agtggtatct gttcactctt cggtggggca gttgcgacaa ctatgggttg gagatggatt
     7141 ttcatcttct caattatcgt tgccgtactt tcaatgttac tcatcaaagg gacgcctgaa
     7201 acgaaatcag aagttaccaa tacacataaa tttgacgttg caggtctaat tgttctagta
     7261 gttatgttat taagtttaaa cgttgtcatt actaaaggtg cagcacttgg ttatacatca
     7321 ttttggttct ttagtttgat tgcaatcgta attgtagcat tctttatgtt cttaaaagtt
     7381 gagaaaaaag tagataatcc gcttattgat tttaaattat ttgaaaataa accatataca
     7441 ggtgcaacga tttcgaactt cttattaaac ggtgttgcag gtacattaat tgtagcgaat
     7501 acattcgtgc aacaaggttt aggttataca gcattgcagg caggatactt atcaattact
     7561 tatttaatca tggtgttatt aatgattcga gttggtgaaa aattattaca aaaaatgggt
     7621 tctaagcgac caatgttatt aggtaccttc attgtaatca ttggtattgc acttatttca
     7681 ttagtattct taccaggcat attttatgtt atcagttgtg tcgtaggata tttatgtttc
     7741 ggactaggct taggtattta tgcaacacct tctacagata cagctatttc aaatgcaccg
     7801 ttagataaag ttggcgttgc ttcaggtatt tataaaatgg cttcatcact tggtggtgca
     7861 ttcggtgtcg caattagtgg tgcagtatat gccggtgcag ttgctgcaac gagcattcat
     7921 acaggtgcga tgattgcact ttgggttaac gtattaatgg gaatcatggc atttatcgca
     7981 attttattcg cgattcctaa tgatgataaa cgtgtcaaag atgcgaaata ataacgatga
     8041 cacgcccagt gttttgataa tgatttaaat gaaaataaag caaattaaat gaggaaatgt
     8101 atagagcagt gcatatagat aaatctatga ttggattaac actaatataa atataaatga
     8161 aaaaccatcc acttaaactt ttgataagtt aggtggatgg ttatttattg cgttgttcta
     8221 aaatttggac atttatatgg aaagaactat atataatttg aaggttatgg caattcgctt
     8281 caatgaaggc gacgtgtaaa agtgtatatt aaacgtttcg gggtcagact ccgagaacag
     8341 tgtccgtcat gcttaccgca tactaatgaa gcattattat atcagtgttt atagccgatt
     8401 taagataata aagtttgcca aacaaaaaag ccaacccatg aatgttggat tggctttgta
     8461 taagcatatt aagttctaat ttttatataa ggtatgaata taaataagac attaaaaatt
     8521 aaatttcagt tgttgcaatt tcttcatctg taggtacatc atcgttaaga ccaacaagtg
     8581 cttcagaaac atttcgtgaa tgataaccga tacgttcaag aacaccaatc atatcgatat
     8641 atagtaatcc gccttttgtt gtacattcac cacgattaag gcgtttaata tgacctttgc
     8701 gtagtttatg ttcaatatta aatgattctc tactacgttc tacaatttca tcttttttcg
     8761 ttttgtcata aacatctaac atgtcgatgg ctttatcaaa tgactcagca acatggttga
     8821 ataatttatc cataccgcgt tgtgcatctt ctgtaatgcg aatatcttca tcatgttggc
     8881 gttttaattg agcgacatac tcttctgtta gctctgctac ttttaaaata gagcgattga
     8941 catcaaacat aactgctaaa cgctcaacgt ctgctttcgt aatggctttt gtagaaattc
     9001 taactaaata atttcgaata ctatcattga ttgtttcaac agcttggtgc ttttgttcaa
     9061 gctttttgat caatttttta tcgtcttttg taatttcgcg aatgtcttca aacattgata
     9121 agacaatctg acccacattt tgtaattctt tttgagtttc ttgtaatgca acaccaggtg
     9181 cgtgataaac aagatctttg tttaagtgct gaggtttata gtcatcagca atatctttac
     9241 ctgggacaag ctttgtaact atccatgcta aacctgctac aaatggtaat tgaatcaaag
     9301 tatttgttat gttgaagata ccatgtgata ctgcaatcgt catcgctggt tttaagtgcc
     9361 atacatcttg taacaaacta atcaaatgaa tcacaactgg caagaaaatt gtgaagataa
     9421 ttaccccgat taagttaaag atgacgtgta caagcgccgc acgttttgca gcgattgagc
     9481 cggctaaact agctaagata gctgtaatcg ttgtaccaat attatcgcct agtaacacag
     9541 ggattgctgc gtttaagcta attaaatctt gttgataaaa ttcttgtaaa ataccaatcg
     9601 tcgcacttga actttgaact agtgctgtta accctgcgcc gacaatgaca gcgagtattg
     9661 gatttgtaga catatcaagc attaattgct taaatccatc taatgatgct aaaggtttaa
     9721 cggcatcacc cataaattct agaccgaaga atagagaacc gaaaccgaat agtatgcggc
     9781 caatgttatt gattttagag cgtttaaaga aaaagattaa aaatgcacct aatgctaaaa
     9841 ttggcattgc atattcgcct aaatctatac cgataataaa tgcagttacc gttgttccga
     9901 tattagcacc cattatcact ccaatggctt gtttcaatgt cataaatcca gctgttacca
     9961 gtccgattgt gataactgtc gtacctgaac tactttgtat taaaatagtt acaacgatac
    10021 ctgcaataac acctaatact ggatttgatg taaatttgtt taaaatatct cgtagcctgt
    10081 ctcctgctga tgcttgaagc ccgtctccca tgatttttaa gccgtaaagg aaaataccta
    10141 aaccacctaa aaaggagaaa atgacttctg taaccgacat ttccattatt tcacctcaaa
    10201 taagctttat atttagatta tcgcttataa ttgtaaattt aatgttaaga ttaggtaaaa
    10261 ttatttaaca atatatgtta tttgtatatg acttgtaaaa tatcatcact tattatgtaa
    10321 attttcagtg tgaaatggca gatttgcaat cacttgttta acaaaatgat gcaatcaatc
    10381 atacaattat gtgtcatcaa aaaaatcatg tgagtgggat aacgaaataa agtttgtgaa
    10441 catatcattt ctatcccact ccatgatttg aaatcaccat atagacatga tttaatgtgc
    10501 ctcgttataa caatctgtgt tcttttaata atgactcaat gtacgtacct tttatctttt
    10561 taaggaatcc tgctaatgcg agtttctgca ttttcgaatc tttagtaatc tcgcgcaaat
    10621 cttgatggtc atttagttcg tatacggcat ccattaagac gcgaagatca aatgtactat
    10681 tgatgacctc tggaatacca cgatctatat ttagtaattg ataaacagct tccatggcag
    10741 tacgaaccga atattctgtt gtaaatacag tatctcgctc tgtttctgca aagttaccaa
    10801 taaatgctaa attttgtgat tgatgcggga cgactaaagg tctgtcgccg atagcacgcg
    10861 tcatgaaata agaggtaata tatggcatat aaacaggaat cgtattagat gcatgttttg
    10921 ctaagtcttc aattttgtca gttggtacac ctaagtgata cagccattct tggcatattt
    10981 cattaccact acattctgtg attggctttt taatataatc gccgtttaca tctgaatata
    11041 aggcataaat ccacgtagat atttcatttt taggttggtt tttaaactgt tgctgacgat
    11101 tgattgtaaa actgatttgc catgcagaat cattgattgt aataataccg ccagtaaccg
    11161 ttttacctgc aagtggatca cgtttacaaa tgctttctat tgtatcgata atatctttat
    11221 tgtttgttgt cgatgttgct gaaacaaacc aacttttttt gggaatattt tggcaaaact
    11281 tatcaggatt accaaattca ggactttgtc gcgctaaatt tttccatagt gtccaactac
    11341 cacctaattc gtcagttggt ggcgctggtg tatcattatc accataagta gagctttctg
    11401 taatactacc gtttgtcaca aagacaagat cgtttatagt cagtttaata gattctgcat
    11461 taccattacg gtcaattaat atttctcggg caattttttg acttgtcgta acatctactt
    11521 taatatcttc tacttttaca tcgtattcaa attgaacccc atgcgatttt aaatattcaa
    11581 ccataggtaa tactaaagat tcatattgat tatatttagt gaattttaaa gctgaaaagt
    11641 ctgcgagacc accaatatga tgaacgaatc gcattagata gcgacgcatt tccatagcag
    11701 aatgccacgg ttcaaatgca aacatcgttt tccagtaaat ccaaaagttt gaattaaaga
    11761 aatcgtcgga aaatacatct gttattttga catcatctaa atcttcttca ttcgttaagc
    11821 ataaatctaa aatttcttta atcgccgttt tagtcaaagt gaaatctcca tctgtgacta
    11881 aacgttgacc ctgtttctca ataacacgac aacgagaata gttagggtct tctttgttta
    11941 accaatagaa ttcatctaat actgacgcat gatcaatttc aagtgaaggg atagatctga
    12001 ataaatccca caaacattca aagtggttct ccatttcacg accaccacgg acaacatagc
    12061 cttttaaagg catattttca ccatcaagac taccgcccgc tttgggtaac tcttctaaaa
    12121 tatgaatctt agaaccttcc atttgaccat cccttattaa aaaacaagct gcagcaagtg
    12181 aagctagacc agatccgatt aagtaagcgg atttgttttc tacattttca ggttttttag
    12241 ggcgcgcaaa tgcttcataa tttccataac tgtaatacat atccaccaag tcctttcgta
    12301 ttataataca ctcagactat acccctttgt gaattgcaaa tattagtatt tatattggta
    12361 aatttgacga aattagatct tttttatatt ttttgatttt taaaatttta tcactcgaat
    12421 gtttttggat tctgaggtgc gggacttgga ataattcgaa tcattatgtg gcatgttaaa
    12481 cagctaaata taagctatgc acaattaaca agcggatact cctgacgcaa ttatagtatc
    12541 attacatatt gtttaatcgt ttggggtaat gtaattaaaa ggtaaaatag gcagagtgtt
    12601 atacataaaa tgtaaagaaa gaggcgatag aatgtctaaa atcaggtctt ttacaatatt
    12661 aagtctactt atttacttag ctatgatgtg ctacacagta gtgacctatt caaaattacc
    12721 aaccaaagta cctattcact ataatttagc aggggatgct gataactttg ctgataaatg
    12781 ggtgctgctt ttgattaata gcgcatttat agtgatttgg cttatatttt tcattgcagg
    12841 tagatactat gaacgatttg ccaaatggtc acattataat catacaccac gtgaaattcg
    12901 agcgattaaa ttatttttaa gtacgttaaa tttagagatt atgagctata tgtctatctt
    12961 cacagtatta gaaatttggc aaatacaaca ccatcatcaa ttcaatttac tatggtttaa
    13021 tatgatattt atcattatca ttggtttgac gcttgtcata ttttgtttac ttcctacaat
    13081 tcataaaatg agagattctc aataaaaata taaactgtca tgatgcagtt ttgaagcgga
    13141 t
//
LOCUS       DAKSQE010000072        13136 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.072, whole genome shotgun sequence.
ACCESSION   DAKSQE010000072 DAKSQE010000000
VERSION     DAKSQE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13136
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.072"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..1205
                     /locus_tag="O0V33_001800"
     CDS             <1..1205
                     /locus_tag="O0V33_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCX8976426.1"
                     /translation="ERLKNTIEIEESKGISRYFDITTGTYIAYIRKSKSEKVIDFFKD
                     NKRIERFSFIDNKVHMKETFNVDNKVCYQVFYDEKGYPYISRNINANNGAVGKTYVLV
                     NKKEFKNNLALCVYYLEKLIKDSKDSIMICDGPGSFPKMFNTNHKNAQKYGVIHVNHH
                     ENFDDTGAFKKSEKYIIENANKINGVIVLTEAQRLDILNQFDVENIFTISNFVKIHNA
                     PKHFQTEKIVGHISRMVPTKRIDLLIEVAELVVKKDNAVKFHIYGEGSVKDKIAKMIE
                     DKNLERNVFLKGYTTTPQKCLEDFKLVVSTSQYEGQGLSMIEAMISKRPVVAFDIKYG
                     PSDFIEDNKNGYLIENHNINDMADKILKLVNNDVLAEEFGSKARENIIEKYSTESILE
                     KWLNLFNS"
     gene            complement(1294..1650)
                     /locus_tag="O0V33_001801"
     CDS             complement(1294..1650)
                     /locus_tag="O0V33_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCX8976427.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
     gene            2141..3100
                     /locus_tag="O0V33_001802"
     CDS             2141..3100
                     /locus_tag="O0V33_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCX8976428.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRIVVLEYSFADYLAALDMKPVGIADDGSTKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHNKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMG
                     IQPEVTKDKTAHVGERKGGPYIYLNNEELANINPKVMILATNGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLQSRGIMASESMAEDLEKIAEKAK"
     gene            complement(3147..3278)
                     /locus_tag="O0V33_001803"
     CDS             complement(3147..3278)
                     /locus_tag="O0V33_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001794249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976429.1"
                     /translation="MRYVTMRQFIKRIVKTILVGYVIKFIRNKLSGKSSHPTDNKHK"
     gene            complement(3342..3557)
                     /locus_tag="O0V33_001804"
     CDS             complement(3342..3557)
                     /locus_tag="O0V33_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HCX8976430.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIIVIFTKKADKDGYIIFPKN"
     gene            3722..4273
                     /locus_tag="O0V33_001805"
     CDS             3722..4273
                     /locus_tag="O0V33_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCX8976431.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDIVLDAIEKS
                     CVDATYRTFYQHVLVYEIENKVADCIISYSGENELKYEKAWEQLDLPEEIKQYGTPLP
                     VKEAKDDEYYIETIATFEAYRGRGIATKLLTSLLESNTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(4325..5263)
                     /locus_tag="O0V33_001806"
     CDS             complement(4325..5263)
                     /locus_tag="O0V33_001806"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCX8976432.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISTLFAGI"
     gene            5445..6806
                     /locus_tag="O0V33_001807"
     CDS             5445..6806
                     /locus_tag="O0V33_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCX8976433.1"
                     /translation="MATGVLEDDIVKEIYDSSKEWVSVEVKLSQSLDPSTLFHLTDNE
                     AGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHL
                     RVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRECFTVEALNDL
                     TDLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESVNNEEKIVLARRRLIKFD
                     KDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKR
                     SQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYH
                     LYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYID
                     IYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
                     GKS"
     gene            6793..8466
                     /gene="menD"
                     /locus_tag="O0V33_001808"
     CDS             6793..8466
                     /gene="menD"
                     /locus_tag="O0V33_001808"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCX8976434.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLKVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTVNRVSWLEKWQRIEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRVTLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNNVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL"
     gene            8453..9256
                     /gene="menH"
                     /locus_tag="O0V33_001809"
     CDS             8453..9256
                     /gene="menH"
                     /locus_tag="O0V33_001809"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCX8976435.1"
                     /translation="MTHYNYYEANVETNQVLVFLHGFLSDSRTYHNHIDKFTDNYHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLERILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDEARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRQQRLSQSPQKMAKALRDYGTGQMPNLWPRLIEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            9249..10070
                     /gene="menB"
                     /locus_tag="O0V33_001810"
     CDS             9249..10070
                     /gene="menB"
                     /locus_tag="O0V33_001810"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCX8976436.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVAE
                     MIDAFSRARDDQNVSVIVLTGEDDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
     gene            complement(10306..10635)
                     /gene="sspC"
                     /locus_tag="O0V33_001811"
     CDS             complement(10306..10635)
                     /gene="sspC"
                     /locus_tag="O0V33_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HCX8976437.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDDTSHNQYHILFIDTAHQRIKFSSFDNEEIIYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
     gene            complement(10673..11854)
                     /gene="sspB"
                     /locus_tag="O0V33_001812"
     CDS             complement(10673..11854)
                     /gene="sspB"
                     /locus_tag="O0V33_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HCX8976438.1"
                     /translation="MNSSCKTRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLE
                     INVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYY
                     YPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLT
                     DEKGFYFEEDGKVRLVKATPLANNIKEKESAKTVSPQLKQELKTTVTPTKVEENEAIQ
                     EDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSE
                     QDLPNCATFPNQMIEYGKSQGRDIHYQEGVPSYNQVDQLTKDNVGIMILAQSVSQNPN
                     DPHLGHALAVVANAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIG
                     Y"
     gene            complement(11936..13009)
                     /gene="sspA"
                     /locus_tag="O0V33_001813"
     CDS             complement(11936..13009)
                     /gene="sspA"
                     /locus_tag="O0V33_001813"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HCX8976439.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQT
                     DKQQTPKIQKGGNLKPLEQRERANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNQFNGAVFINENVRNFLKQ
                     NIEDINFANDDHPNNPDNPDNPNNPDNPNNPDNPNNPDNPDNPNNPDNPNNPDNPNNP
                     DQPNNPNNPDNGDNNNSDNPDAA"
BASE COUNT         4663 a         1812 c         2388 g         4273 t
ORIGIN      
        1 cagaaagact aaaaaatacg attgaaattg aagagagtaa aggtatttca agatattttg
       61 atataacgac tggtacatat attgcctaca ttagaaaaag taaatctgaa aaagtgattg
      121 atttctttaa agataataaa cgaattgaac ggtttagttt tatagataat aaagtgcata
      181 tgaaggaaac atttaatgta gataataaag tttgttatca agtattttat gatgaaaagg
      241 gatacccata tatttcaagg aatattaatg ctaataatgg tgctgtaggt aaaacttatg
      301 tgttagttaa taaaaaagaa tttaaaaaca atttagcact gtgtgtttac tatttagaaa
      361 aactaataaa agattctaaa gatagtatta tgatttgtga tggaccaggg agttttccaa
      421 aaatgtttaa tacaaatcat aaaaatgctc aaaagtatgg cgttatccat gttaatcatc
      481 atgaaaattt cgatgatacg ggtgcattta aaaaaagtga gaaatatatt attgagaatg
      541 cgaataaaat taacggtgta attgtattaa cagaggcaca aagattagat attcttaatc
      601 aatttgatgt agaaaatatt ttcactatta gcaattttgt taagatacat aatgctccaa
      661 aacattttca aactgaaaaa atcgtaggtc atatttctag aatggtacca acgaagcgaa
      721 ttgatttgct tattgaagtg gctgagttag tcgtaaaaaa agataatgct gttaaatttc
      781 atatatatgg agaaggatct gtcaaagata aaatagctaa aatgattgaa gataaaaatt
      841 tagaaagaaa tgtttttctt aaaggatata caacaactcc acaaaaatgc ttggaagatt
      901 ttaaattagt tgtttctaca tctcaatatg aaggtcaagg gttaagtatg atagaagcaa
      961 tgatttctaa aaggcctgtt gttgcctttg acatcaaata cggaccaagt gattttatag
     1021 aagacaataa aaatggttat ttaatagaaa accataatat taacgacatg gctgataaaa
     1081 tactaaagct tgttaataat gatgttttag cagaggagtt tggttcgaaa gcgagagaaa
     1141 acattataga aaaatattca acggaatcaa tattagaaaa atggttaaat cttttcaata
     1201 gctaaatgaa aaaatcctag gtattcatga gtacctagga tttttacgca tgatatatac
     1261 aaaaaaagag aggatagtcc tctcaaataa tagttaataa aattgtagcc atttcttcca
     1321 gctgtaacta ctaatacaag ctaagttcat tacgatgtgt atgatttcaa attcataacc
     1381 acttacatat cctcttgtaa gtttaagtgt gaagattgag cctaacataa cacaaatata
     1441 aaaaacatta acataaggcg ttaaaaagcc gataataagt gctaaacctc caacaacctc
     1501 accaattgac atcaatactg cgatggtagg tggtaaacct aaagacccta agaagtgaat
     1561 tgttccatct aaatctacaa atttcaatgt gccgtgtact aagaatgatg ttgctagcat
     1621 ccatctaatt aatagcatac ctaatttcat catgtcacct ccaacataca tttttttctt
     1681 aaaacatagt ccaaattata tttgtaaaga agtggaaagt aaagattatt tataaaaata
     1741 attattaaat atttattaaa tgttctattg tattgaacaa ttgttgtaga aagatgttta
     1801 agtcatcagt tactttttgt atttataaat taagattagt acagcatagt gcaaagttta
     1861 taggtaaatg aattaattat ggcatcaaaa tatacgtatc ttgaacactg ttgtggaggc
     1921 ttagtagtag tgagaacaaa cagaatacta ctttaagcga tatgattaat attaaccagt
     1981 gcgtcatcat caaaattaga ggccatttgc tgtttataat acgaagattt gataatcaac
     2041 tatgataaga aaaaataatg accgtttaaa tatattataa gtttattaaa tgataaaata
     2101 ataatatagt tctctataaa aattgtttgg aggtaaatgt gtgaatagga atatcgttaa
     2161 attagttgtg tttatgctaa tcttagttgt agcagtagcg ggttgtggtc aaaaagatac
     2221 tgaagagaaa actgaaatga cgacgataaa agatgaatta ggaactgaaa aaattaagaa
     2281 aaatccgaaa cgtattgttg tattagaata tagttttgct gattatttag cagcattaga
     2341 tatgaaacct gttggtattg cagatgatgg tagtactaaa aatataacaa agtcagtaag
     2401 agataagatt ggggcatatg aatcggttgg atctagaccg caaccgaata tggaagtgat
     2461 aagtaaatta aaaccggatt tgattattgc agatgtcagc agacataaga aaatcaaatc
     2521 agaattgagc aaaattgcgc cgacaatcat gttagttagc ggtacgggag attacaatgc
     2581 aaatattgaa gcatttaaaa cagtcgctaa agcagttggc aaagagaaag aaggcgaaaa
     2641 gcgtctggaa aagcataata aaatattagc ggagattaga aagaaaattg aacagagtac
     2701 gttaaaatct gcatttgcat ttggtatctc aagagcaggt atgtttatta ataatgaaga
     2761 tacatttatg ggacaattct taattaaaat gggtattcaa cctgaagtca caaaagacaa
     2821 aactgcgcat gttggtgaac gcaagggtgg cccttatatt tatttaaata atgaagagct
     2881 tgccaatatc aatccaaaag ttatgatttt agctacgaat ggaaaaacag ataaaaatag
     2941 aacgaaattc attgatcctg cagtttggaa atcattaaaa gctgtgaaag ataataaagt
     3001 atatgatgtt gatcgaaata agtggttgca atcaagaggt attatggcaa gtgaaagtat
     3061 ggcagaagat ttagaaaaaa ttgcagaaaa agcaaaataa aaatacagcg ccactcgtaa
     3121 atcatataag agtggcgctg tattttttat ttatgtttat tatctgtcgg atgtgatgat
     3181 ttacctgaaa gtttatttcg aataaattta attacataac cgacaaggat tgttttaaca
     3241 attcttttaa tgaattgacg catcgttaca tacctcattt ctctttatct tatgtactat
     3301 atacccattc atatatgctt tttaaacgtc attgtcacaa tttaattttt agggaatata
     3361 atataaccgt ctttatctgc ttttttagta aaaatgacaa taatggcatg tattattgaa
     3421 atgatggtag ggatacctgt ccagaaaaat aataagtgaa aaagaccttg tccaaattta
     3481 tcagcataaa atttatgaat acctaaacct ccaagaaata atgcaacaat aacataaatg
     3541 gctttattga ctttcatttg taaatcctcc ttaattataa ttctacttaa attcgttgtg
     3601 aaaaccaatc tttctaactt tagaattttc aaactttcta aaattataag tatatctttt
     3661 taaaataagc tagaatttct atataataaa tgttaataac gtaaaaggga atgatgacat
     3721 agtgatacgt caagcacgtc cagaggaccg atttgatatt gcgaagttag tttatatggt
     3781 ttgggatgat atggaattag aattggtaaa gcatctacct aaagacatag tattagatgc
     3841 aattgaaaaa agctgtgttg atgcaacata tagaactttt tatcagcatg ttttagttta
     3901 tgaaatagaa aataaagtag cagattgtat tattagctat agcggtgaaa atgaattgaa
     3961 atatgaaaaa gcatgggaac aacttgactt gccagaagaa ataaaacaat atggcacgcc
     4021 attacccgta aaagaagcta aagacgatga gtattatata gaaacaattg cgacatttga
     4081 agcatataga ggtagaggta tcgcgacaaa gttattaacg tcattacttg aatcaaatac
     4141 acatgttaaa tggagtttga attgcgatat taataacgaa gcagcattaa agttatataa
     4201 aaaagtaggc tttatatctg atggacagat tgaattatat aagcacatgt atcatcattt
     4261 aattgttaaa taaaaaaact cgacagttcg atgtaagtcg attgccgagt agaatcatct
     4321 tatattaaat gcctgcaaat aatgtactaa tataaatacc tagtgcatat aataaaccga
     4381 aaaatgtatt tgttttacca gcagcagcca ttgctggcat cattgtaggc ggtgtatcat
     4441 tcttcttgaa acgtctgata actttaacag gcattgggaa tgataacaac gcaagtaagt
     4501 aaaataatga gccaccaggt ttaataatga tcgtaagtac aataaatgca taagcgataa
     4561 agtacatgat tgccataaat gttaaagaag catttttacc taatagaatg ggtaaagttt
     4621 tacgaccact tgctttatct ttgacacggt cgcgaatatt gttggccata ttaattaaac
     4681 cgatagtgat tactataggt acacttaacc aaattacata gctttgaata ttgccagttt
     4741 gaataaagaa tgcaataacg ataataaaca tacccataaa tacgcctgag aataattcac
     4801 cgaaaggcgt ccatgaaata gggaaagggc cacctgtata taggtaacca acagccatac
     4861 atactaatcc aactggtaat aaccaaaatg aagagttagc agctaaaaac aaacctaata
     4921 tcgctgctaa gatgtaaaat gcaatggcta atcgtagcac aagctctggg ctcataccgt
     4981 tgcgtacaat tgcaccacca atgcctacag attcatgatc atcaaggcct tttttataat
     5041 catagtattc gttaaacata ttagttgctg cttgaataag taagcatgct agtaacatgg
     5101 caatgaatag gctgatttta atatgatctt cgctaccaag aaaatatatt ttagatgctg
     5161 ctgtaccaac taaaacgggt actacggaag cagttaatgt atgaggacgc attaaatgcc
     5221 aatatttctt aactgtagaa tattgctgat attgattact cattgaatac aaaacctctt
     5281 taaatcatat tttttataac aaagatattt taatagaaag tagcataaag gtcaattgac
     5341 gtgaaaaatt gcaagataag tttaagcaat tcttgtaaca caattatcca aaaatgatac
     5401 aatataataa tgagtgaaaa aagacgaaga aagaagtgaa atggatggct acgggcgtat
     5461 tagaggacga tattgtcaaa gagatatatg acagctcaaa ggaatgggtt tcagttgaag
     5521 tgaaattatc acagtcacta gacccgagca cattatttca tctcactgac aatgaggcag
     5581 gagatcgctt ttatatgcgt ttgaatgata atcgaacgtc atattttggc tacaaagcaa
     5641 ttcaattatt caaaaataat tctaaaaata aacaatctat ttttaaagac tgggaaaaat
     5701 taaaacataa catcacattt atacatccgc aatctgagaa acatcatctt cgagttgttg
     5761 gagggttcca attttcaagt cataaatcag atgatgaatg gcgagagttt ggactaaatc
     5821 attttgtatt acctgaagtt ttaatttcaa cagataataa tgggacattt ttaacttata
     5881 cagttaaaag ggaatgtttt actgttgagg cattgaacga tttaacggat ttgttcaaca
     5941 atatatcgga tatagatgtg gacgagcaaa ttggggaaat tactagaaat gaagatattt
     6001 ataaagatga ctggcgtcaa cttgtagtag aagcaataga atctgttaat aatgaagaaa
     6061 aaattgtact agcacgtaga cggttaataa agttcgataa agatatcagt attccatata
     6121 ttctaaagca agcatattct aaagaaaaaa acagttatat attcttgtta gaatcacaag
     6181 attctatatt cttttcacag acacctgaac aattaataaa ggtcaataat aaaatactat
     6241 cgactaaagc tgtagcaggt acaattaaac gttcacaaga tgaggacgaa gatacaaaaa
     6301 atgttgaagc atttttaaaa gataataaaa acttaatcga acatcgattt gttgttgaca
     6361 gtattttaca tgatattaaa ccttatatca ctgaattaca ttatgataag acgcctaaaa
     6421 ttctaaaaaa tgatcattta tatcacttgt acactgaaat aaaggcgcca ctgaaggatg
     6481 attcgtatat tagtttaatt gataatttac atccaacacc tgctttaggt ggctatccaa
     6541 aagaatttgc gatggatttt attgaacaga aagaatttgg tacacgagga ttatatggtg
     6601 cgccggttgg ctatatagat atatatgatg attgtgaatt tattgttgca attcgttcaa
     6661 tgcttattaa gaaagcacaa gcaactttat ttgctgggtg tggcattgtt aaagattctg
     6721 atccagatag tgaattggca gaaacgaacc ttaagttcac acctatgatg aatgcattag
     6781 gagtcgatat gaatgggaaa tcataaagca gctttaacga agcaagtttt tacatttgca
     6841 tctgagttat atgcgtacgg cgtaagggaa gtagttatca gtccgggatc acgctcaacg
     6901 ccacttgcac ttgcatttga agcacatcca aatattaaaa catggataca ccccgatgag
     6961 cgaagtgcag cgttttttgc agtggggtta attaaaggca gtgaaagacc tgtcgctata
     7021 ttatgtacgt caggtacagc agcagcgaat tatacgccag caattgctga aagccaaatt
     7081 agtagaattc cattaatcgt tttaacaagt gaccgtccgc atgaattaag aagtgtaggc
     7141 gcaccacaag cgattaatca agtaaatatg tttaataatt atgtaagtta tgagttcgat
     7201 atgccgattg cggatgatag taaagagacc attgatgcaa tttattatca aatgcaaatt
     7261 gctagtcaat atttatatgg accacataaa gggccgattc attttaactt gccatttaga
     7321 gatccgttaa cacctgattt gaatgcaaca gaattgttaa cttctgagat gaagatttta
     7381 ccgcactatc aaaaaagtat agatgcatcg gcattaagac acattttaaa taagaaaaaa
     7441 ggtttaatta ttgtaggaga tatgcagcat caagaagttg atcaaatact gacgtattca
     7501 acgatatatg atttgcctat tttagctgat cctttaagtc atttaagaaa atttgatcat
     7561 ccgaatgtta tctgtacata tgatttgctg tttagaagcg gcttagactt aaaagtggat
     7621 ttcgtaattc gtgttgggaa accagtgatt tctaaaaaat tgaatcaatg gttaaagaaa
     7681 actgatgcat ttcaaatatt agtgcaaaac aatgataaga ttgatgtctt tccaatagca
     7741 ccagatattt catatgagat ttctgcgaat gatttcttta ggtcattaat ggaagacacg
     7801 acagttaatc gagtaagttg gttagaaaaa tggcaacgca tagagaaaaa agggcgtaaa
     7861 gaaattaaat gttatttgga acaagctaca gatgagagtg cattcgttgg tgaattgatt
     7921 aagaaaacat ctgaaaaaga tgcattattt attagtaata gtatgccaat cagagatgta
     7981 gataacttgt tattgaataa aaatatagat gtctatgcga atcgtggtgc aaatggtatt
     8041 gatggtatcg tatcaactgc acttggtatg gctgtccata aacgagttac attattgata
     8101 ggtgatttgt cattttatca tgatatgaat ggactattaa tgtcaaaatt aaataatatt
     8161 cagatgaata ttgtattatt gaacaacgat ggtggcggta ttttttcata tttaccacaa
     8221 aaagaaagtg caaccgacta ttttgaacgg ttgtttggca caccgacggg attggatttc
     8281 gagtatacag ctaagttata tcaattcgat tttaaacgtt ttaacaatgt ttcagaattt
     8341 aaaaatgcca cattgttatc tgaaacttcg acgatttatg aattgataac gaatcgcgaa
     8401 gataacttta aacagcatca aattttatat cagaaattga gtgaaatgat tcatgacaca
     8461 ttataattac tatgaagcaa acgttgagac caatcaagtt ttagtatttc tgcatggatt
     8521 tcttagcgac agtcgtactt atcataatca catcgataaa tttactgata actatcatgt
     8581 catcactata gacttaccag gacatggcga agatcagtct tcaatggatg aaacgtggaa
     8641 ttttgattat attacgacgt tgttagaacg aattttagat aaatataaag ataaatcaat
     8701 aacattgttt ggatattcaa tgggtgggcg tgttgcatta tattatgcaa ttaatggtca
     8761 tatccctata tctaatttga tattagaaag tacgtcacca ggtattaaag aagaagcaaa
     8821 tcaattggaa cgtcgccttg ttgatgaagc gcgtgctaaa gtattagaca tagcaggtat
     8881 tgaattattt gttaatgatt gggaaaagtt gccattattt caatcgcaac tagaattacc
     8941 agttgaaata caacatcaaa taagacaaca acgattgtct caatcgccac agaaaatggc
     9001 caaagcatta agagattatg gtacaggtca aatgccaaac ttatggccgc gcctgataga
     9061 aattaaagta ccaacattaa tattagctgg agaatatgat gaaaaatttg tacagattgc
     9121 gaaaaaaatg gcaaatttaa ttcctaatag taaatgtaaa ttaatttctg ctacaggtca
     9181 tacaattcat gtggaagata gtgatgaatt tgatacaatg atattaggat ttttaaagga
     9241 ggagcaaaat gactaacaga caatgggaaa cacttagaga atatgatgaa atcaaatatg
     9301 aattttacga agggattgct aaggtaacaa taaatcgccc tgaagtacgc aatgcgttta
     9361 cacctaaaac agttgctgaa atgattgacg cattttcacg tgcacgtgat gatcaaaacg
     9421 tttcagttat cgtattaact ggggaagatg atttagcatt ctgttctggt ggtgaccaga
     9481 agaaacgtgg acatggtggt tatgtaggtg aagaccaaat ccctcgctta aatgtattag
     9541 atttacagcg tttaattcgt attattccaa aaccagttat cgcgatggta aaaggttatg
     9601 ctgtaggtgg cggtaatgta ctaaatgttg tttgtgactt aacgattgct gctgataatg
     9661 ctatttttgg acaaactggt cctaaagtag gttcatttga tgcgggttat ggttcaggat
     9721 atttagcacg tatcgttgga cataagaaag cacgtgaaat ctggtactta tgtcgtcaat
     9781 acaatgcaca agaagcttta gatatgggcc tagtaaatac agtggtacct ttggaaaaag
     9841 ttgaagatga aactgtgcaa tggtgtaaag agattatgaa acactcacca actgctttac
     9901 gattccttaa agcagctatg aatgctgaca cagatggttt agctggttta caacaaatgg
     9961 ctggggatgc aacattgctt tattacacaa cagatgaagc gaaagaaggc cgtgatgcgt
    10021 ttaaagaaaa acgtgatcct gacttcgatc aattccctaa attcccataa gttattttga
    10081 atatgatatt aagtcacttg cgtcgtttat tagcgacgat gggtggcttt tttattttat
    10141 aagtatttaa aagggcattt ttattaatta tcataaagat aagttagttt gagaaaaatt
    10201 aatagagaaa gaataaatgt taaagattat tacaatgaaa aaacgagctg gtgagaaatt
    10261 gaattctcgc caacccgctt tatgaagtga aatcatttaa attagttata ctaagcgctc
    10321 ataaacaatt ggtcgcgaag tgccaatacc ttgtttggat gacgtttgca tgaggatatg
    10381 ctgtgtatca tcataatcaa gaatataaat gatttcttca ttatcaaaag atgaaaattt
    10441 aatgcgttga tgtgccgtat ctataaaaag aatatgatat tgattgtgac ttgtatcatc
    10501 gccatgacgt atacaaattg atttttgtaa ttgatgatta ctccaattac caatgaataa
    10561 attgatattg gtttgttcta gatgtgtgag tttggttgtg tcgtaaacta aatttataaa
    10621 ttgtagttga tacatagttt tacctgcctt taatcaatat ctatattact ttttagtaac
    10681 ctatcattga accataccag ttataatcac gattgaatga taaatgtaat aggcttgaat
    10741 ctgcatcttg gattgataat tctgtatccc aaggattcca gtaaataagt ttttcttggt
    10801 cattaatttt agcattagca acaactgcta gggcatgtcc taaatgtggg tcattaggat
    10861 tttgagatac actttgtgca agaatcataa tacccacatt atcttttgta agttgatcaa
    10921 cttgattata tgatggtacg ccttcttgat aatgaatatc tctaccttgt gatttaccgt
    10981 attcaatcat ttgattaggg aatgttgcac aattaggaag gtcttgctca cttacttcag
    11041 ggtataatgt acgcataata tcatgtgcat tataagtgtc tgtattttta gttgcattta
    11101 ataacgctgc catactgaat cctgcacacc atgagttatc gaattgttgt tctctaattt
    11161 tgaagttttt tagtgtgttt tcatattgaa cttgatcttc ttgtatcgct tcgttttctt
    11221 caacttttgt aggtgttact gtagttttta gctcttgctt taattgtggt gaaactgttt
    11281 tagcactttc tttttctttg atgttattag ctagtggcgt agcttttact aatctaactt
    11341 tgccatcttc ttcaaaataa aatccttttt catcagtaag aacagtgata tttgaatttt
    11401 tatctttaat ttggtctaaa tctttagcga tgaagtttga aattttaaca ctgtaattca
    11461 tgtcttcttt tgatttgttt aaatcatctt tatttttagg gctaagtgtt aaagtataaa
    11521 caattttacc gtcttttata actggataat agtaactatt atcttcttcg ccattaaatt
    11581 tataaatttt aaatgcttca ccaagttcgt atttaccagt ttttgcatta ctttgtttat
    11641 ctaatgcttt agcataacca gcaaattgtt gttgtgctag atcttttact ttttgaggta
    11701 ctttgtcact cttaacatta atttctagct gtttagagtg tgaatcggct ttggctttat
    11761 tgttattagc aaatgcgcct agtgataaaa taagcattga aaccattatg atgcttataa
    11821 tattgaatac tctagtttta catgaactat tcatataata ccctccattt aagtcaaaat
    11881 attttgcgtt gttaagtttt tgcttctata tactaaatcc aaattaagat gaagtttatg
    11941 cagcgtcagg gttgtctgaa ttattgttat cgccattgtc cgggttattt gggttgttag
    12001 gttggtcagg gttatttgga ttgtccgggt tgttagggtt atcaggatta ttaggattgt
    12061 ctggattatc agggttgtta gggttatcag ggttgttagg attgtccgga ttatttggat
    12121 tgtctggatt atcagggttg ttagggtggt catcatttgc gaaattgata tcttcaatat
    12181 tttgttttaa gaagttgcgt acattttcat taataaatac tgcaccgtta aattgatttg
    12241 gaacgccacc ccaatgaatg ccaatgactt catttttttc attaaataca ggtgaacctg
    12301 agttaccacc agttgtactt aaatcatatt gcattgcttc accttttaag tacgttattt
    12361 ttcctttact ttcccacatt gttgcgacag gtttatcacc aggatatcct gttacagtaa
    12421 tattttggtt aacttgtgtt tcagcattat tactcattgt tgctggttta actacttcgc
    12481 caatatgttt gttttgctca ttaggggaga atttaacgat tgctaaatca ccttcgcctg
    12541 aatatttagt gatttgttca gcagtgaaac caccattagg ataattgtct tggttaattg
    12601 cagaagggaa tgcttttaaa gcatgaggat caccgtgcgt agcatctacg acgtgtttat
    12661 ttgttaaaag tgtatcttta cctacaacta caccagaagc aataaatgta ccagtaggtg
    12721 cttcaacttg aatataagta acaggtgcat aatgaccatt cgttgtatct gtgatttggt
    12781 gacgatcgtt atttggtaat ataacattag cgcgttcacg ttgttctaat ggtttaaggt
    12841 taccgccttt ttgaatctta ggtgtttgct gtttgtctgt ctgcgtttgt tgtggatgat
    12901 tgtccatagc ttttgaagat aacgcatttg ctgctggaga actcacaagt gtcgctgttg
    12961 tcaaagttgc aacgaataaa gaactaactt ttaaaaattt acctttcatc taaaaacctc
    13021 caaaaaattt atttacaagt taaatataac actaaaaatt tttaagtcaa taagaatata
    13081 taaataaatt aaaaagtttc tgtaaagatt agagtttaca aaaacattaa gagaat
//
LOCUS       DAKSQE010000073        13031 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.073, whole genome shotgun sequence.
ACCESSION   DAKSQE010000073 DAKSQE010000000
VERSION     DAKSQE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13031
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.073"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(297..1010)
                     /locus_tag="O0V33_001814"
     CDS             complement(297..1010)
                     /locus_tag="O0V33_001814"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCX8976440.1"
                     /translation="MAKLNVEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
     gene            1269..1571
                     /locus_tag="O0V33_001815"
     CDS             1269..1571
                     /locus_tag="O0V33_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976441.1"
                     /translation="MSRSKKYFYLSSLMIILSFFFNTNNVFLSGLFNSFIKLILFCSV
                     INSIVLILSIIFADRSIKSLKPDADWIRIASKSLPWIILIVILVHIFSIVHTFGFI"
     gene            1826..2191
                     /gene="crcB"
                     /locus_tag="O0V33_001816"
     CDS             1826..2191
                     /gene="crcB"
                     /locus_tag="O0V33_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCX8976442.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTS
                     YVFGILLCYVGIKLGGGLS"
     gene            2188..2541
                     /locus_tag="O0V33_001817"
     CDS             2188..2541
                     /locus_tag="O0V33_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HCX8976443.1"
                     /translation="MISIILVMIGGGLGAIARSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLTIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFNINLFLNYSLL
                     QFIIGFIACYIGYHI"
     gene            complement(2791..3624)
                     /locus_tag="O0V33_001818"
     CDS             complement(2791..3624)
                     /locus_tag="O0V33_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCX8976444.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNKVKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQHIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHGVVTIPKSVTPNRISENFRIFDFELSDEQMTRIDGLNQDKRIGPDPKTFE
                     G"
     gene            complement(3836..4744)
                     /locus_tag="O0V33_001819"
     CDS             complement(3836..4744)
                     /locus_tag="O0V33_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HCX8976445.1"
                     /translation="MDLSSPVVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKHLGAKYTPTSILYFNPKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
     gene            complement(4869..6062)
                     /locus_tag="O0V33_001820"
     CDS             complement(4869..6062)
                     /locus_tag="O0V33_001820"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCX8976446.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHADDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVK"
     regulatory      complement(6168..6279)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            6434..8026
                     /gene="pckA"
                     /locus_tag="O0V33_001821"
     CDS             6434..8026
                     /gene="pckA"
                     /locus_tag="O0V33_001821"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCX8976447.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKTQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
     gene            complement(8319..9065)
                     /locus_tag="O0V33_001822"
     CDS             complement(8319..9065)
                     /locus_tag="O0V33_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HCX8976448.1"
                     /translation="MPIESFTHQFEEITYLSDDLQVKALMMTPHHEVKRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTELLRLLHDKYP
                     QAFIHMVGFSRGGLQGLLTFQDLPVNSYIIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPAILIVHGGKDQQVGIHHAYYLADQLELKGAMHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMNQVELHR"
     gene            complement(9070..9543)
                     /gene="ytkD"
                     /locus_tag="O0V33_001823"
     CDS             complement(9070..9543)
                     /gene="ytkD"
                     /locus_tag="O0V33_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HCX8976449.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEIADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAITRELYEETGAKVKNIHYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            9609..9866
                     /gene="yidD"
                     /locus_tag="O0V33_001824"
     CDS             9609..9866
                     /gene="yidD"
                     /locus_tag="O0V33_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCX8976450.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(9863..10864)
                     /gene="menC"
                     /locus_tag="O0V33_001825"
     CDS             complement(9863..10864)
                     /gene="menC"
                     /locus_tag="O0V33_001825"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCX8976451.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELNDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQMFHVLLSFSVAYGATASGLSNKQLESLKATKPTRIKLKWTPQIMHQIRVLR
                     ELDFHFQLVIDANESLNRQDFTQLQLLAREQVLYIEEPFTDISMLDDVVDGTIPPIAL
                     DEKATSLSAIINLIKLYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGVKVVVGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(10869..12347)
                     /gene="menE"
                     /locus_tag="O0V33_001826"
     CDS             complement(10869..12347)
                     /gene="menE"
                     /locus_tag="O0V33_001826"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCX8976452.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMRSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGTDITTNGLLDNTMGIQYDTSNETVVPKDSPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYDLQK
                     ILLGGAKLSASMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANV
                     DVKIKNPNKKGHGELMIKGANVMNGYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGIIDAVCVGHPDDTWGQVPKLYFVSESNISK
                     AQLIAYLSQHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG"
     gene            complement(12506..12961)
                     /locus_tag="O0V33_001827"
     CDS             complement(12506..12961)
                     /locus_tag="O0V33_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HCX8976453.1"
                     /translation="MKICLMLYLLIFLSSCGTNNKIVGQRFEIQQHNEIIGRIYFSAD
                     YAHIRGIEKGTVKYFVDKVGSKRYLFIEYIPDNVLNCKPDFWKTLKYKKDKVTYYVYL
                     IENLDDELFHLSALQDMNHRPIDIADDVATMDKSPHQNDRITLKLNKNN"
BASE COUNT         4067 a         2483 c         1933 g         4548 t
ORIGIN      
        1 agattttttt catagctatt ttttattgtt atcgagccta tcccagactc tctatactgt
       61 tatattaaca gtcattgact gaatgataat aagcttttca attatagagg ccccaacata
      121 gagaatttca aaaaagaaat tctacaggca atgcgcgttg gggtgtgggg ccccaacaca
      181 gagaatttcg acaagaaatt ctacaggcaa tgaaatacat attaatttaa aattgactcc
      241 aatatgaaaa acccccataa gcacaatgcc tatgagggtt taaatatatt tatatattat
      301 aaaatagaaa gacctgaaga ttgaatatct ttcgcaaagc ctttaactgt atctactgat
      361 aattcgttaa tatcgcgacc taagtttgta ttcacttttt tcacaacatc tgcagggcat
      421 gtaataatat ctgcaccaat ttcatcagct tgaatcacat tgaataattc gcggcaactt
      481 gcccataata atttaacgcc gtctttacta tgcgtaactt ttacagcctc tttcattaat
      541 ggtaatggat ctacgcctgt atctgcaata cgtcctgcaa atactgaaac atatgttggc
      601 acaccttcag ttactgcttc agttatttct ttaacttgtt caattgtata aacagccgta
      661 acgtttaatc tcacattgtc agctgaaagt tttttaatta aaggaatcgt tgattcacct
      721 tttgtattta caataggaat tttaacaaat acattttcgc catattgttt taaaattgct
      781 gcttcttttt ccatagtttc taaatcgtct gcaaatactt caaatgaaat tgaagcatct
      841 ggaatttctt tcacagcttc ttcagcaaaa gctttgtaat ctgttacgcc cgctttcgcc
      901 attaaactag gatttgttgt aaagccatcc acttgtttgt ttttataagc tgctttcatt
      961 tcttcaatat ctgcaccatc cgcaaatact tctacattta gtttagccat ataatatagc
     1021 ctccttgatt cttattaaaa ttttaacaac atctgcatgt ctttttctta caaccatttg
     1081 taaaaaatga tttttatttc tttgttatat aaaacattta tatcacgctt ttataatata
     1141 tagctaattt tttgcctata tttcttaaaa atgactttag caacattgtg tcaacaaggt
     1201 aattaaacta ttgttttgtg attgtttatg atacgatatg taaatgataa ccatccagga
     1261 ggtctagcat gtctcgttca aaaaaatact tttacttatc tagcttaatg attattttaa
     1321 gctttttctt taatacaaat aacgttttcc taagtgggct ttttaattct tttattaaat
     1381 taatactttt ctgcagtgtt attaactcaa ttgtactaat attgtctata atttttgcag
     1441 atcgttcaat taaatcacta aagcctgatg cagattggat tagaattgcg agtaaaagtt
     1501 tgccttggat tattctaatc gttattttag tacatatctt ttcgattgtt cacacattcg
     1561 gttttattta aaaaagataa aatgtcaatg tagccttaat acaaaacaat acaatgtatc
     1621 atgctaaaat gagtaaatcc atttgatgac gttgttgcgt ataaaaatat tagattttcg
     1681 aaatcataac tatgcatcta atcgctatag ttatacaaca agatataaca tataatgagg
     1741 tttgataatg catcgacaat ttttgtcgtc gcgttgccaa aacctctttt ttaaattcaa
     1801 actacttctt ttcgaggtga accaaatgca atatgtatat atttttatcg gtggtgcttt
     1861 aggcgcttta ttacgttacc tcatttcttt tctgaatact gacggaggtt ttccaatcgg
     1921 aacactgata gccaatttga ctggtgcttt tgtaatggga ttgctaacag ccttaacaat
     1981 tgcatttttt tcaaaccatc cgacccttaa aaaagctatt acgactggtt ttcttggtgc
     2041 tttaacgact ttttcaacat ttcaattaga attaatacat atgtttgatc atcaacaatt
     2101 tataacttta ctactatatg ctgtaacaag ttatgtcttt ggtattttgt tatgttacgt
     2161 cggtataaaa ctaggtggtg gtttatcatg atatcaatca ttttagtcat gattggcggc
     2221 ggtttgggtg caattgctag aagtgccatt actgattatt tcaatcataa attcacttca
     2281 aagttaccta tcgcaacatt gatagtaaat cttgttggta gttttttaat tggattaact
     2341 ataggcttat caatttcaat ctcatggttc cctgcgttct ttgttaccgg ttttttaggt
     2401 ggcttaacaa ctttctcaac attagccaag gaacttacac taatgatgac gccaaaattt
     2461 aatattaacc tttttctcaa ttattcactt ttacaattca tcattggatt tatagcttgt
     2521 tatattggct atcatattta aaaataaaat gcttcattca gcaaataggt aaattacgac
     2581 accttcctga acgaagcatt ttttaatttt catgcaaatt ttttagcacc agataatgcc
     2641 tatcaaaata aaacaatctt tgttgccgtt taaataatgt gaatgtcaat aaattctcca
     2701 aactagtcga aaataaaaac gttaagcaat atgtctatta cctaacgttg acaatttatc
     2761 tgaccttttc atcagttgag cgattttaat ctatccttca aaagtttttg gatcaggtcc
     2821 aattctctta tcttgattta aaccatcaat tcgcgtcatt tgttcatctg ataattcgaa
     2881 atcaaatatt cggaagtttt cagagattct gtttggtgtc accgatttag ggattgtaac
     2941 cactccatgc tgcacattcc atcttaaaac aacttgggca ggtgactttc ctaattcttg
     3001 agcaatgtct ttaattgtct catcatttaa aatttgtgca ttcatcaatg gtgaccaaga
     3061 ttccatcacg atatgttgtg ctgccaaata taatttcaat ttatgttgcg ttaaatatgg
     3121 atgatattca acctgattaa ttacaggttt aattgacact tgtgccaaca aagcttccaa
     3181 atgttcaggt tcaaaattgc tgacacctat atttttaact ttattatttt tatataaatc
     3241 ttccatacct ttccatgtat caaccattac ggcttcgttc gtacctggcc aatgtactag
     3301 atacaaatct aagtatttta aacctaatct agataaacta gcttcgtagg cagctgctac
     3361 attttcacga ccaaaatcct caaaatataa ttttgaagta ataaataagt cttctctagc
     3421 aataccagtt gattccaatc cggcacgaat gccagcacct acttgttctt cattcccata
     3481 aacttttgcg gtatcaatac tacgatatcc ttgttcaatg gcatacttaa cactttccat
     3541 gcaattttca tcattttcca cacgaaatgt ccctaaacca atttgtggca tcgtgtttcc
     3601 attataaaat gttttaacct ccataaatat cgcctcacct ttttgatgta ttataccctg
     3661 ttatcataac aaatctgagt tgaatacatg agaaaaaaca cttagagcaa tcaaccacta
     3721 aaatgctagt aatatctctc aaatattaat caaattgtaa aagtaattct gtttaattta
     3781 tgacaaacta aaaaagccga agtaacaaca tatagtcatc acttcagcct aacatttaat
     3841 tgaatgattc aattttatcc atcatttgtt gtaagtcttc cacgttgtat tgaatacgac
     3901 catggaatac aaatttgtta aagaactcgt ctaattgttc agcaccgaca agcactttga
     3961 cagcactatt ttgattataa tttgaaattg ttacatcgcc ttcattttta ggattaaagt
     4021 ataaaattga agtcggtgta tatttagcac ctaaatgttt ttgtaagtct tcagccaatt
     4081 gtttaattgc ctcaatttga tctgaataat ttacaaatga taatgaacgt ttgtcatcat
     4141 tttgatccat aacaatagtt tgcggtctag atttatctaa atccaatgta tcaaatactt
     4201 gttccatagg tggtaaatct ttaaattgac cgccacttat accattataa acatgacctt
     4261 ttaacaattg agaatcaata atataaagac cagttcttgt taatactaaa tgactaattc
     4321 gttcaatatt attaaagcca tcctttggta aaaagatatt tgccataatg tgcatatctt
     4381 ctggtcgaat tcgtttttct ttaactaatc tttcacgaat accaattaat ctcatgtccg
     4441 ttacatattc actatgattt ttcgagaaca attttaatgc gtcaatctca cgatcttttg
     4501 tactaaccat gtgattataa tcttcttgtt gttttgtaat tgtcttttta ttttgaatac
     4561 gctctttctc taaagcttct tcatgagact ttttaatgtt ttgttcttgt tgttcatact
     4621 tttcttctgt ttgtcgctta acttttttct tactacctaa ggcaactaag aaaaggacaa
     4681 aaaagattaa tgcaatgact actgcaataa tgagtccaat gactaccggt gaagataaat
     4741 ccatcacaac aacgctcctt tttaatatat gaataacttt aattataata gaaaagctaa
     4801 agattttcga tacatattat catttatata ccgaaaatct tttatttagc tatattcaat
     4861 tcatcttatt attttactgc gtcttttaat tcttccactt tgtctaattt ttcccatggg
     4921 aataagacat ctgtacgtcc aaaatgacca taagcagcag tttgtttgta aatcggttgt
     4981 ttcaaatcaa gcattttaat aatacctgca ggtcttaggt caaagtgttt tctaactgct
     5041 tcgacaagtt gcccttcaga aactttacct gttccaaatg tatcaattgc aattgacact
     5101 ggttctgcaa caccaatcgc atatgccaat tgtacttcac attgatctgc taaacctgct
     5161 gctacaatat ttttagccac ataacgtgca gcgtatgcag ctgaacggtc tacttttgta
     5221 ggatctttac cgctaaagca tccgccacca tgacgtgcat agccaccgta cgtatcaaca
     5281 atgattttac gtcctgttaa tcctgcatca ccttgaggtc caccgattac aaagcgtcct
     5341 gtaggattga tgtagaattt agtttgttca ttaatcaagt tttctggaac agttggataa
     5401 atgacatgcg ctttgatgtc ttcttgaatt tgttcaagtg tcacatcatc agcatgttgt
     5461 gttgatacga caatcgtatc aatacgtaca gggttatcat tttcatcata ttcaacagtg
     5521 acctgaactt taccgtctgg tcgtaaataa tttaacgtac catctttacg cacatctgat
     5581 aaacgttttg ccaattgatg tgataaataa attgctagag gcatatacgt ctctgtttca
     5641 ttcgttgcgt aaccaaacat taaaccttgg tcacctgcac ctgttgcttc aatttcttct
     5701 tcgctatctt tatcacgata ctctaatgct ttatccacgc cttgtgcaat gtcaggtgat
     5761 tgttcatcaa tcgcagttaa aattgccatt gtttcataat cataaccata ttttgctctt
     5821 gtgtatccaa tttctttgat tgtttctcta acaactttcg gaatatcaac atatgttgtt
     5881 gtagaaattt cgccggcgat caatgccata cctgttgtaa cagttgtttc acaagctaca
     5941 cgtgcatttg gatcgtcttt taaaatagca tctaatattg catctgacac ttggtcagcg
     6001 attttatctg ggtgtccttc tgtaacagac tctgaagtaa ataatcgttt gttatttaac
     6061 atagtttgct cctttaaatt tatattacga aaattctctc tctgtgagct aaataaaaaa
     6121 gaccttctaa ctattaatat agagagaagg cctaatacgt ccattcgctc ttatcgttca
     6181 gacctatttg tctgcaaacg gtttggcacc tttcttttat aaaaaaagag gttgctgggt
     6241 ttcattgggt ccatgtccct ccaccactca ggataagaga atccgttaaa aataatacta
     6301 cctaattaat gaattaatgt caatttttca caaataaatt tacagtaaaa tattgtagat
     6361 taattatgtt aatgtgttat actaattaaa tgtaaaggct tacatttaaa ttatcgcttt
     6421 ggagggattt aggatgtcag tagacacata cactgaaaca actaaaattg acaaattact
     6481 gaaaaaacca acgtcacatt ttcaactttc gacgacacaa ctttataata aaatcttaga
     6541 caataacgaa ggggtattaa cagaacttgg tgctgttaat gcaagtactg gaaaatatac
     6601 tggtcgttcg cctaaagaca aattttttgt ctcagaacct tcatatagag ataacattga
     6661 ctggggagaa attaatcaac ctatcgatga agaaactttc ttgaagttat accataaagt
     6721 actagactat ttagataaaa aagatgaatt atacgtattt aaaggctacg ctggtagcga
     6781 taaagataca atgttaaaac ttacagtcat caatgaatta gcatggcata atttatttgc
     6841 taaaaatatg tttattagac ctgaatcaaa agaagaagct acaaagatta aacctaactt
     6901 cactatcgtt tctgccccac attttaaagc agatccagaa gttgatggta ctaaatctga
     6961 aacctttgtc attatttcat ttaaacacaa agtcatttta atcggcggta ctgaatacgc
     7021 tggtgaaatg aaaaaaggta tcttctctgt aatgaattat ctcttaccga tgcaagatat
     7081 tatgagcatg cattgctcag caaacgttgg tgaaaaaggc gatgttgcat tattctttgg
     7141 tctatctggc acaggtaaaa caaccttatc ggctgaccca caccgtaaac taatcggtga
     7201 tgatgaacac ggctggaata aaaacggggt ctttaatatc gaaggtggct gctatgcaaa
     7261 agcaattaat ctttccaaag aaaaagaacc acagattttt gacgcaatca aatatggtgc
     7321 aattttagag aacactgtag ttgcagaaga tggttcagtg gactttgaag acaatcgtta
     7381 tacagaaaac acgcgtgccg cttatccaat taatcacatt gacaatattg tagtaccatc
     7441 taaagcagca catccaaata caattatttt cttaactgcg gatgcatttg gtgttattcc
     7501 accgatttca aagttaaata aagaccaagc aatgtatcat ttcttgagtg gtttcacttc
     7561 taaattagct ggcacagagc gtggtgtgac agaacctgaa ccatcattct caacatgttt
     7621 cggcgcaccg ttcttcccgt tacaccctac tgtttacgct gatttattag gtgaacttat
     7681 cgatttacat gatgttgatg tttatcttgt taatactgga tggactggcg gaaaatatgg
     7741 tgtaggacgt agaatcagct tacattacac acgtcaaatg gtaaaccaag cgatttctgg
     7801 caaattgaaa aatgcagaat atacaaagga tagtacgttt ggtttaagca ttcctgtaga
     7861 aattgaagat gtaccgaaaa ctattttaaa tccaattaat gcttggagcg acaaagagaa
     7921 atataaaaca caagcagaag atttaattca acgttttgaa aagaacttcg aaaaatttgg
     7981 tgaaaaagtt gaacatattg ccgaaaaagg tagcttcaac aaataaattt aaatactaat
     8041 taaagagcca accgctgtta aattttaaca atggttggct cctcttattt tatatggtaa
     8101 aaatttatag gcaattttat tacaacaatc acatttaagg tgaccttcat gccaaactca
     8161 catcactcat tcaatggaag cagcacgtct tcacataaag taccgattcc ttacgctatg
     8221 catgttgccc cactttgcat aaagtaccga ttcctcactc aacgtatatc acacatctcc
     8281 aaagattgat agttcccatg cgcacaccac gtttcatact acctatgcaa ctcaacttgg
     8341 ttcataaatt ctttaatata agtcaatgtt tcaaccatcg ctggtggtct tggcacatgt
     8401 ccttctgcca tttgataaaa tgtttcatgc atggcacctt ttaactctag ttgatccgct
     8461 aaataatacg catgatgaat accaacttgc tggtctttcc ctccatgtac aattaatatt
     8521 gctggactgt tttcattaat gtttggaatc gcttggcgtg cctcatatgc tgctcgatct
     8581 tttttcggat gaccaatcat tctacgtaac atccctctta aatcaacgcg ctcttcatac
     8641 atcaaatcaa tatctgagac tccaccccag atgatataac tatttacagg taagtcttga
     8701 aacgtcaaca acccttgtag tccacctctt gaaaaaccaa ccatatgaat aaaagcttgc
     8761 ggatatttat catgtagcaa cctcaataat tcagtcacat catttaaatc gccacgataa
     8821 aattcatctt taccttcact cccattgtta cctcggtagt atggcccaat cactaaagtt
     8881 tgactatctg aaaattgcat taatctacct gcgcgcacac gtcctacttg gcctttgcca
     8941 cctcgcaaat atactacaat gcgttttact tcatgatgtg gtgtcatcat taaagctttt
     9001 acttgtaaat catctgacaa atatgtaatt tcttcgaatt gatgcgtaaa agattcaatt
     9061 ggcattcgtt tacgtttgat aaaatccaag tgattgcacc ctctctacgc attttaaaat
     9121 ggtactatct tgcagtaaga aactccgttg tgcgagttca atatcattga tacagttaaa
     9181 caacactggc cctgctgttt ctaaataatc gttcttgctt accaatgatt caacttcgat
     9241 aaaatataca tcttttacaa aatcagtttg atcatgtgtt tcaatggtat attgcgctat
     9301 gtaatgaata tttttaactt tggcgcctgt ttcttcatat aattcacggg taattgcttc
     9361 agcactactt tccccgcgtt cccttttacc accaggaaat tcaatacccc gtaaattatg
     9421 tttggtaaaa agcaattgat ttttaaacgt tggaatagct agcacatgat tgccatctgc
     9481 tatctcatta tcctttttaa atgtcaaatt aacttgacga ttatctttat ccctaaactt
     9541 cacgcgcatc acatccctac attgtatgtt aatataatag ttaattacta tcgttggagg
     9601 cattaattat gaaaaagata ttcttggcga tgattcattt ttatcaacgt ttcatttcgc
     9661 cactcactcc accaacttgt cgtttttatc caacatgttc agagtacact agagaagcga
     9721 ttcaatacca cggtgctttc aaaggccttt atttaggcat ccgtcgtatt ttaaaatgtc
     9781 atccgcttca taaaggtggc tttgacccag ttccgttaaa aaaagacaag tcagcaagca
     9841 agcattcaca taaacataac cattaatatg gttgtaattg agttatatcc actaaagggg
     9901 ggcgaaattc gagtcgcccc tcttttaata tgcctgaatg cgccaccaca tcttgttcaa
     9961 aataataacc tgctggtgta acatctcctg gataatcacc tttacgagca agcatcgctg
    10021 taaaataacg gcttaaacca tattcataca tgccgccaac aaccactttt acaccatgac
    10081 ttttcaaagt atcaattgcc gtttgcactt tatcaatgcc acctagacga aatggtttta
    10141 atacaacaac tttcacattg tataatttta tcaaattaat tatggccgac agtgacgtcg
    10201 ccttttcatc aagagcaatt ggaggtattg ttccatccac tacgtcatca agcattgaga
    10261 tatctgtaaa tggctcttcg atataaagaa cctgttcacg cgctaataac tgtaactgtg
    10321 taaaatcttg acgattcaag gattcatttg catctataac caattgaaag tggaagtcta
    10381 attcccgtaa cactctaatt tgatgcatga tttgaggcgt ccattttaat ttaattctgg
    10441 tcggctttgt tgctttcaat gactctagtt gtttatttga taagccgctc gctgtcgctc
    10501 catatgctac tgaaaatgaa agcagtacat gaaacatttg atacaatgcc atgacaatag
    10561 ttgcccttgc agcaggcgta ttttccaatg aatctactag ttttagtgca gcttcatacg
    10621 tttcaaatga tttattccta ttatcttcga accattgctc aattacatgt ttcactgacg
    10681 caattgtttc atgatcatac caatctgttt gaaaagcgtt acattccccg aaatatgcat
    10741 ttcctttatc atcattcaat tcgataaaca aacaatcacg atgcgttaaa gtgactttcg
    10801 gtgttacaat ttgtgactta aatggctcac tatatttata aaaatgcaaa gctgtcaact
    10861 tcatcaaatc atcctctata caacttattt ctttgtaatt tacctgttga tgtataaggt
    10921 aaagtatcaa ccttttcaaa gtgtttcggt actttatatt tcgctaaatg ttgtgataaa
    10981 tatgcaatca attgtgcctt tgaaatgtta ctttcactga caaaatataa tttaggtact
    11041 tgtccccaag tatcatcagg atgccctaca catactgcgt caatgatacc tggaaattgc
    11101 ttcgctaccg tttcaatttg atatggataa atattttcac cgccactaat aattaaatct
    11161 ttacgtctgt cataaatcat gacataacct tcatgatcta tttcagcaat gtcacccgta
    11221 ttaaaataac cattttcaaa cgtacccgtt aaatctgttg gatacaaata tccattcatc
    11281 acattggcgc ctttaatcat taattctcca tgaccttttt tattaggatt tttaattttt
    11341 acgtctacat tggcacttgg cattcctaca gtgtcaggac gtgcatgcaa catttccggt
    11401 gttgctgtta aaaattgcga acatgtctca gtcatgccaa atgaattata aattggcagg
    11461 ttatattgta atgccgtctc tatcatactg gcagatagtt tagcaccgcc gagtaatatt
    11521 ttttgtaaat cataaggttc atgtaaacct tgttgcataa gccaatttaa agtttgtggc
    11581 acaagcgaaa tgtgtgtgat gcgttcattt tttatcatcg ttaaaatttg ttcggcattg
    11641 aatttatcaa caatgcgcac agtaaatcct tcaataacag ctcttaaaag tacactgaga
    11701 cccgaaatat gataaatcgg caagacagat agccaattag tatcacgatc aaatcccaag
    11761 ctctctttac atccgattgc actggcataa tgattacgaa acgtttgcgg caccgctttt
    11821 tgaggaccag ttgtccctga tgtaaacata atcgatgcaa tgtcatctaa attaaatgat
    11881 gtatttaata tgttggacgg cgaatctttc ggcaccacag tttcattcga tgtatcatat
    11941 tggataccca ttgtattgtc caacaaaccg ttcgttgtaa tatccgttcc agcgaattca
    12001 atatcatcca gcgatacaat ttgaaaccct cgcaattcca gtggcaaggt gcaaaaaatc
    12061 aattgtacat cgattgacct catctgattc gtcatctcat taggtgtcaa ccttgtatta
    12121 atcatcgcaa tttcaatatt tgccaaccaa catgcatgta ttaaaatgat tgattgaatc
    12181 gaattatcta tgtatagccc gacacgagat tgttgataag ccttgagcct tttagccaat
    12241 agactcgctt cacagtataa attttgataa gtataagatt cttgaccgtc tgttatcgca
    12301 atatgatgtc cattttgttg tgcttgttta tataaccaaa agtccatgcg ctattcctcc
    12361 aaaatcattt acattataat tataacgatt ttatgacatt ctagcagtgg ttatgtttaa
    12421 aaatataaaa aagtagacga attgatgcat tgatatgatt gttataatgc tcaatacata
    12481 tcgttatatc attcgtctac tattatcagt tatttttatt taattttagt gttattctgt
    12541 cattctgatg tggtgattta tccattgttg ccacatcatc tgcaatgtca atcggtctat
    12601 gattcatatc ttgtaatgca cttaaatgaa atagttcatc atctaaattt tcaatgagat
    12661 atacataata tgttaccttg tcctttttat attttaacgt tttccaaaag tccggcttgc
    12721 aattcaatac attatccgga atatattcaa taaataagta acgtttgctg cctactttat
    12781 ctacgaaata ttttacagtg cctttttcta tacctcttat atgtgcatag tctgctgaaa
    12841 agtaaattct acctattatt tcattatgtt gttgtatttc aaatcgttgg cctactattt
    12901 tattgtttgt gccacaacta cttaaaaaaa tcagtagata tagcattaaa catattttca
    12961 tccccttgaa ttttaaaaac tttttcaaaa gcaacacctc taaaaataat tacactatat
    13021 tatatcaaat t
//
LOCUS       DAKSQE010000074        12937 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.074, whole genome shotgun sequence.
ACCESSION   DAKSQE010000074 DAKSQE010000000
VERSION     DAKSQE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12937
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.074"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..114)
                     /locus_tag="O0V33_001828"
     CDS             complement(<1..114)
                     /locus_tag="O0V33_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCX8976454.1"
                     /translation="MIINLETQQIHFDLEHETPYFQSPEKNRIRLDIDVLNK"
     gene            complement(116..205)
                     /locus_tag="O0V33_001829"
                     /pseudo
     CDS             complement(116..205)
                     /locus_tag="O0V33_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            208..534
                     /locus_tag="O0V33_001830"
                     /pseudo
     CDS             208..534
                     /locus_tag="O0V33_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343723.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            571..762
                     /gene="vraH"
                     /locus_tag="O0V33_001831"
     CDS             571..762
                     /gene="vraH"
                     /locus_tag="O0V33_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HCX8976455.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALAMIVIVLSTITTPIIGI
                     PVGLLGGAYYLKRREEKGK"
     gene            1154..1354
                     /locus_tag="O0V33_001832"
     CDS             1154..1354
                     /locus_tag="O0V33_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCX8976456.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            complement(1481..2050)
                     /locus_tag="O0V33_001833"
     CDS             complement(1481..2050)
                     /locus_tag="O0V33_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCX8976457.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKVQRSLNLWAYVMLIFMTLATILTGPLVVY
                     WNWAWGITVIVIYGIGFYASMKIDDFKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNI
                     KTSVLSFIAFVGAFILIALISMYLANKFL"
     gene            complement(2310..2705)
                     /locus_tag="O0V33_001834"
     CDS             complement(2310..2705)
                     /locus_tag="O0V33_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCX8976458.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIIVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            complement(2773..3126)
                     /locus_tag="O0V33_001835"
     CDS             complement(2773..3126)
                     /locus_tag="O0V33_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCX8976459.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
     gene            complement(3736..4575)
                     /gene="noc"
                     /locus_tag="O0V33_001836"
     CDS             complement(3736..4575)
                     /gene="noc"
                     /locus_tag="O0V33_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HCX8976460.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            complement(4618..5337)
                     /gene="rsmG"
                     /locus_tag="O0V33_001837"
     CDS             complement(4618..5337)
                     /gene="rsmG"
                     /locus_tag="O0V33_001837"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCX8976461.1"
                     /translation="MTVEWLAEQLKEHNIELTETQKHQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFELPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            complement(5337..7214)
                     /gene="mnmG"
                     /locus_tag="O0V33_001838"
     CDS             complement(5337..7214)
                     /gene="mnmG"
                     /locus_tag="O0V33_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCX8976462.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILEILEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQSKLQRVS
                     D"
     gene            complement(7281..8660)
                     /gene="mnmE"
                     /locus_tag="O0V33_001839"
     CDS             complement(7281..8660)
                     /gene="mnmE"
                     /locus_tag="O0V33_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCX8976463.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVVEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEIIGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            complement(8804..9157)
                     /gene="rnpA"
                     /locus_tag="O0V33_001840"
     CDS             complement(8804..9157)
                     /gene="rnpA"
                     /locus_tag="O0V33_001840"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCX8976464.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            complement(9278..9415)
                     /gene="rpmH"
                     /locus_tag="O0V33_001841"
     CDS             complement(9278..9415)
                     /gene="rpmH"
                     /locus_tag="O0V33_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCX8976465.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            10146..11507
                     /gene="dnaA"
                     /locus_tag="O0V33_001842"
     CDS             10146..11507
                     /gene="dnaA"
                     /locus_tag="O0V33_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCX8976466.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            11785..12918
                     /gene="dnaN"
                     /locus_tag="O0V33_001843"
     CDS             11785..12918
                     /gene="dnaN"
                     /locus_tag="O0V33_001843"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCX8976467.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
BASE COUNT         4197 a         2399 c         1901 g         4440 t
ORIGIN      
        1 tttattgaga acatcaatat ctaagcgtat cctatttttc tcaggtgatt gaaaataggg
       61 agtttcatgt tctaaatcaa aatgtatttg ttgtgtttca agattaataa tcattttaat
      121 ctccaattct ttttaggtta gcatcgtatg taccacttct tgttttcttg ctacctaaat
      181 tcttaatact gcttgcacct ttccatttta ccttctcata tatttaaatc aattactttt
      241 gattgtggta aggagttctc aaactggaaa acaatatgta actgtcacga tatatctata
      301 ttttttgctg atccaggcac gccatcacag cgaggtttaa atgaacattc taacggaata
      361 cttagacgca gtggattaga taaagaacta gatttcaatt tagtaacgga tgatcatatc
      421 atcagtgttg ctcaaaaaat caatcatcgt cctagaaaat ctttaggtta tagaacacca
      481 ttggaagtat ttatgagttt catcgaagat gataaactgt ccagcttaat ttgacaatct
      541 aaagatttat aaaaaaggag agattgaatc atgaaattta gagaattagt aaaacaatca
      601 tacgaggatt tgaaaaattt gactgtaact tggtataacg tgattgcttt agcaatgatt
      661 gttattgtct taagcaccat tacaacacca ataataggta ttccagttgg tttattaggt
      721 ggcgcttatt atttaaaacg ccgtgaagaa aaaggtaaat aagcgcattc catcagtata
      781 gaacattgct atgacctttc aagtcagatt ttaatatcaa caagtagcgt ttatcgctgc
      841 ttgtttttta tgaattgctt ataaaatcgc catgtttaaa tgatatgtcg ctttgaaaac
      901 tagcaaacac cataaaagac tacatttttt tatttttata aaccccttta aatcaacgtt
      961 tctaaggttt ttaaaaatct tttttaattt attttcaaaa aacactgtac attatgccaa
     1021 tatgagcgta tagttggact tacgtaataa aagctcgtga attaaattgt agtgtatttg
     1081 tttagaatat cctctttttt agttatgaat ttgttacaaa tattaagtgc aaaagcacac
     1141 ggaggttttc tatatgaata acggtacagt aaaatggttt aacgcagaaa aaggttttgg
     1201 tttcatcgaa caagaaaatg gcggagacgt attcgtacat ttctcaggta tcgctagcga
     1261 tggctacaaa actttagaag aaggtcaaaa agttactttc gaaatcactg aaggtcaacg
     1321 tggagaccaa gcagttaacg tacaaactgt ttaatcttac aaaataaaac gactcattat
     1381 aaatgaatcg ctttgattac caacaaggtt ctaaatcttg ttggtatttt tttatgctat
     1441 tttggataat ataaataata tgaggccata ccacgatgat ttataaaaac ttatttgcca
     1501 gatacatact aatcaaagct ataagaatga atgcaccaac aaatgctatg aaagaaagta
     1561 cacttgtttt tatatttcta gctttagtcg tttgtacttc actaggatct tttccattca
     1621 taaaagcaac aattcgatcg tagttatcca ttttatgcac ttttttaaag tcatctattt
     1681 tcatagatgc ataaaaacct attccataaa tgactatcac cgttataccc caagcccaat
     1741 tccaataaac aactaaaggt cccgttaaaa ttgtagctag tgtcatgaaa ataagcatca
     1801 catatgccca taagttaaga cttcgttgca ctttaatatc aggtacaaat ggaatagtcc
     1861 cttttactaa atcatctaaa gttacattga acaattcgca catcattaat aagttatgta
     1921 tgtctggtaa gcttttgtca ttttcccaat tagaaatact ctgcctagat acatataact
     1981 tttcagcaag atattcttgt gaataaccat ctcgttcccg atacttttta atttgtttac
     2041 tcagattcaa ttacatacgc ctccagtcaa ttagtacaat cattacaatc agactatcag
     2101 ataaagttaa ttaaagtcac ctgtatgatt gactaaatgt tttgacatac gacaacatca
     2161 gggttcgaag acgcccttaa gacagacctg tatgattacg cgcttgtaat tttctcaatg
     2221 ccaacttgaa tttcatttat taatgatgca acttttccat gctattcaat gcttgcgaag
     2281 ttagtcctat ttaagagatt acgttcacac tatttactat tcgattttcc agctttccaa
     2341 ctatgctttt ctaatctttt ttgtgctata aattctaccg cttcaaaaat acatacggca
     2401 ctgatgaacc agcttaggaa accaacaata atgctaatta accacatgtg cgtcatatgt
     2461 aacatcatcc atgtaactaa aatacaaact aatacccctg tcataccaaa cactgcgcca
     2521 cgacttacat gcacagcgac tttatcacca tattgcatac ctgtaataaa catagacact
     2581 aaaaatactg ccggaaacgt tgcaaatata ccgccaaatt ctttccaagg tagtgttaca
     2641 gatacaatat aacttaataa tactgcaaat ccccccacaa aaaatttcat taatgttaat
     2701 ttcatgatgt tctgcctcca ataatttatt acttaataac tgaatacaac taattatcac
     2761 gcattttcca aactataaat gtctaatact gaatattgcg agggaaataa caaaccaaca
     2821 aacaactgtg aatattgcgc ctttccgata acctttatgc ttaatataga gagatgtcaa
     2881 aaatacagtt aatatacaag acagaatacc gacaattgct ccagtactaa gattcatcga
     2941 catctccact aattgcgtac cacgatgatc caaagctaat gcgataatag ccgcaagaaa
     3001 gactgccggc atagtagcta taatgcctcc taacttaccg cctaccttat cagcaataat
     3061 tgaagctaat gcaacagcga taccaccaat gacaaaatga agaattgcac ttccaattga
     3121 aaacatcttt aacgtacctt tctataatta attcaataca aaacgtatca ttcacacaaa
     3181 gtgtaaaact taaattttct actgtaaagg caactcaagc gatgttcata aagctttcat
     3241 gatactttca taaactagca ataaagggtt caaataaagg tatctccagc tcaaattagg
     3301 gagtgggaca gaaatgatat tttcgcaaaa tttatttcgt cgtcccaccc caacttgcac
     3361 attattgtaa gctgactttc cgccagcttc tatgttgggg ccccgccaac ttgcacatta
     3421 ttgtaagctg acttttcgtc agcttctgtg ttagggccct caccccaact tgcattgcct
     3481 gtagaatttc ttttcgaaat tctctgtgtt ggggcccctg actagaattg aaaaaagctt
     3541 gttacaagcg catttttgtt cagtcaacta ctgccaatat aacttcgtag agcatagaac
     3601 attgatttat gtaccagcct gatcaacata taaatataaa tttttatgtt tcacgtaaaa
     3661 caaaaaaaga caaagctgtt atgatcctag ctttgtcttt tatgtgttag tcatcatgta
     3721 tacgacatcc tactactaac gtttatatat tcgaattttt atttcataat aatcttcatg
     3781 atctttgtct ttatgctcaa catgtaatcc tgtttgttga atcgcttgga tacttttgcc
     3841 tacctcatct cgagcttgag tgacatcttg tgcaaagcga aggttttgtg ctttgacttt
     3901 ttctggcccc gttttttggc gtactctatc ttcagtctgt ttcacattta gtttttgtgc
     3961 aatgacttgc tcaatcaacg cttcttgttc gctatcagac aatgataata ccgctctcgc
     4021 atggcgttca gtaattttac cttctcttaa gcgaagtagt actttcggcg ccaacttcaa
     4081 taaacgcaac ttatttgcaa taaagctttg acttttacct aaactttttg ccaattcact
     4141 ttgcgttgta tcaccaattt ccaataattt cttataggct tccgcttctt caacaacaga
     4201 caaattttct ctttgaatat tctcaattaa tgccacaaca gccgtctctt catcatccat
     4261 atcacgaata ataacgtctg cttgaggtaa atttagtgat tgtattgctc taaatcggcg
     4321 ctctccagca ataatttcaa acatatcttc ttcaatcggt cttacaacaa taggctgtaa
     4381 taaaccatgt tcatgtattg attcagcaag ttctttaatt ttatttggtt caaacacctg
     4441 tcttggttga taacggttgg gaacgatacg ttcaatttga atggattcaa cattactatt
     4501 gcgatcttct tcaatatgtc caatgatgtc atctttgttt ttcaaaccaa ataattttga
     4561 aaaaggtttt ttcattatcc attcgctcct tttatgttag ttgtaaagga ttatgcatta
     4621 tttttcaagt aaaggagtct tattaggcgt ccctggtttt cttggatact ttttcggcgt
     4681 ctgtcttttt ttatcaataa tgaacatctg gcgctctcca gcatcttctg gcaattcaaa
     4741 ggtatgtgtt tctgtaacgt taccacctaa cacactaatt gcaaattttg cttcttctaa
     4801 ttcttcttca ccttttgaag attttaatgc aacaaactgt ccaccttttt taactaacgg
     4861 taaacacaat tcactcaaca cggataatct agctactgct cttgcagtaa cgacatcata
     4921 agactccctg tagacaccct taccaaatgt ttctgctcta tcgtgtataa agctgacatc
     4981 ctgtaattgt aattctgacg ctaaatggtt taaaaattga atacgcttat ttaatgaatc
     5041 aacaatcgtc acttttaact gcggaaacat tatttttaac ggaatacttg gaaaaccagc
     5101 tccagcgcct acatcacata tacttatagg ctgattaaaa tcaaaataaa aactaggtgc
     5161 aatggaatca taaaaatgtt tcaaatatac atcgtgttca tctgtaatac ttgtcaaatt
     5221 catcttttca ttccattcaa caagtaaacg ataatatgtt tgaaactgat gtttttgagt
     5281 ctcagttaat tcaatattat gttcttttaa ttgttctgct aaccattcta cagtcattag
     5341 tcactcaccc tttggagttt actttgttct aagtaaatca ataatataga tatgtctgct
     5401 ggatttaccc ctgatattct agaagcttgt gcaatattta aaggttttac ttctgataat
     5461 ttttctcgcg cttcagtcgc caaactatca atcttactat aatctaagtc ttctggaatt
     5521 ttcttctctt ccatacgctt aactttctca acttgttgta gtgatttatt gatataacct
     5581 tcatattttg tttgtatttc tacttgttct tcaacatctg cattcaattg atgttcttct
     5641 tctaaaattt ctaaaattat atcgtaagtc atttcaggtc tgcgtaataa atcgatagct
     5701 aaaataccat cttttaagcg agaaccacca tgttgttcaa taatcgcttg cgtatgttcg
     5761 tttggtttaa tacgaatatc tgataaacgc ttaatttccg catcaatttg ctgacgtttt
     5821 tcattaaaac gtgcatatcg ttcttcagaa atcataccaa gttcatatcc catatccgtc
     5881 aatctcaaat cagcattatc atgacgtagt aacaaacgat attctgcacg tgatgttagt
     5941 aaacgataag gttcattagt acctttagtt acaagatcat cgattaatac accaatatat
     6001 gcatctgaac gacttaatat cttttcgcct gtgtttaaca ctttacctgc agcgttaata
     6061 cctgccatca atccttgtcc tgctgcttct tcataaccag atgtaccatt aatttgacct
     6121 gcagtgtata agtttttaat cattttcgtt tcaagtgtag gccataactg cgttggcaca
     6181 atcgcatcat attcaattgc gtagccggca cgcatcatat ctgctttttc aagacctggt
     6241 attgtctcta acatttgacg ttgcacatgt tcaggaagac ttgtagacaa tccttgcaca
     6301 tatacttcat ttgtattacg accttcaggc tctaagaaaa gttgatgtcg cggcttatca
     6361 ttaaatcgaa caaatttatc ttcaattgaa gggcaataac ggggcccggt tcctttaatc
     6421 atccctgaat acattgcaga tagatgtaaa ttatcatcga taactttgtg tgtttcagca
     6481 ttagtatatg ttagccaaca tggcaattga tctaatatat attctgttgt ttcaaagctg
     6541 aatgcacgac ctacatcgtc acctggttgt atttcagtct tcgaatagtc aattgttttt
     6601 gaatttacac gcggtggtgt acctgtttta aaacgaacaa tatcaaaacc aagttctctt
     6661 aaattatctg ataatgtgat tgatggtaat tggtgatttg gtccacttga atacttcata
     6721 ttacctaaaa tgatttcacc acgtaaaaat gttcccgttg taataattac tgctttagat
     6781 aaatactctg taccaatatt tgtacgtaca cctttaactt cattatcttc tataataagt
     6841 tcgtctacca taccttgcat tatatgcaaa ttctcttcat cttcaatcac gcgtttcatt
     6901 tcttgttgat aaagtacttt atctgcttgc gctcttagtg ctcttacagc aggtccttta
     6961 ccagtattta acattctcat ttgaatgtgt gttttatcga ttgtttttgc catttgtcca
     7021 cctaaagcat caatttcacg aacaacgata cctttagctg gtccacctac agatgggtta
     7081 catggcataa atgcaatatt atctaaattt attgttagca ttaatgtttt agcaccacgt
     7141 cttgcagatg ctaaacctgc ttctacacct gcatgtcccg cacctataac gattacatca
     7201 tattcttgaa ccacaatata aacctcctta tttgatatct tactagcctt cttaagacgg
     7261 tattccgtct atttcaatta ctatttacct aagcagaatt gactgaataa ctgatcgatg
     7321 agttcatcac ttgcagtctc accaataatt tctcctatta tttcccaagt tctagttaaa
     7381 tcgatttgta ccatatccat aggcacacca gattctgctg catcaatcgc atcttgtatc
     7441 gtttgtcttg cttgttttaa taatgaaata tgtcttgaat tagaaacata agtcatatct
     7501 tgattttgta cttctccacc aaagaacaaa tctcgaattt gtatttctaa ttcatcaata
     7561 ccttcttgtt ttaacattga agtttgaatt aatggcgtat cacctatcat atctttaact
     7621 tcattaatat ctatattttg ctctaaatcc attttattaa caattacgat tacatcttca
     7681 tttttaacca cttcatataa tgtgtaatct tcttgagtca atgcttcgtt attgtttaat
     7741 acaaataaaa ttaagtctgc ttggctaaga gcctttctag agcgttcaac accaatcttc
     7801 tctactatat cttctgtctc acgtatacca gcagtatcaa ctaatcttaa tggcacgcca
     7861 cgaacattga cgtactcttc taagacatct ctagtcgtac ctgctacctc agttacaatc
     7921 gctttattat cttgtattaa attatttaac atagatgatt tacctacgtt tggtttacca
     7981 acaataactg tagataaacc ttcacgcata attttaccct gcgcaccggt atctaataaa
     8041 cgattaattt cctgtttgat ttctttagac tgctctaaaa gaaattcagt agtcgcatct
     8101 tcaacatcat cgtattcagg ataatcaata ttcacttcca cttgagcgag tatctctaat
     8161 atagattgac gttgtttttt gattaagtca cttagacgac cttcaatttg attcatcgca
     8221 actttagaag ctctatctgt tttcgagcga ataaagtcca taactgcttc agcttgagat
     8281 aaatcaatac gaccatttaa aaaggcacgt tttgtaaatt cacctggctc agccattcta
     8341 gcgccatatg tcatagtaag ttccagcact ctattaatcg ttaaaatacc accatgacaa
     8401 ttaatttcta taatatcttc gcgtgtaaat gtttttggtg ctcttaacac agacaccata
     8461 acttcttcaa ccacttcttt agactctgga tcaataatat gaccgtaatt aatcgtatgt
     8521 gatggaacat catttaaaag atgttttcct ttatataatt tgtcagcaat ttcaacggct
     8581 tgcggtccag acaatcgaac aattccaatt gccccttcac ccattggtgt tgaaatactc
     8641 gtaattgtat ctaaatccat attactactc gcctccttca acgatgtgaa tacattttaa
     8701 agtaagttat tatatccctg aggttagtct tacgtttgtc tgaggtaaga cttcggggtg
     8761 tgttgagtgg ttaatgtttt actccctttc ccctacccta tccttactta atctttttat
     8821 taaaaacttt ggcaatttta agtacgtgct caagactatt ctgtatttgt aaagtcgtca
     8881 tatctttagc tggctgtctt gctattacaa taatatcttt ggccaatata tgcgacttat
     8941 gtactttgaa attttcacgt attgctcttt taatcttgtt tcttaacact gcattaccta
     9001 gttttttaga aacactaata cctaagcgaa aatggtctat ttctttatta ttacaagtgt
     9061 atacaacaaa ttgtctgttg gctacagaat gacctttttt atatattctc tgaaaatctg
     9121 cattcttttt aattcggtaa gctttttcca ataacatcac tcgcttattt atcgttttta
     9181 tttgaagcta tatttaaact tctattgagc ttataacata aatttctatt tattcttaat
     9241 ttaaacgaaa aaaaagatca ctgatgagtc agtgatctta tgcagataaa actttacggc
     9301 ctttacgacg acggcgcgct aaaactttac ggccattttt tgtgctcatg cgttttctga
     9361 aaccatgaac tttactatgt ttacgtttat ttggttgata agtacgtttt accatgcaaa
     9421 acacctccat ctttatacag ttatctaata taccagatta tgcagactct gatcacttta
     9481 atagataaga ttttgcgttt cttagattaa aattctcgta ttagctcatt gattatctag
     9541 tcataattca agcaactact acaatataac aaaatccttt ttataacgca agttcatttt
     9601 atactaccgc tcaatttttt tacttttatc gattaaagat agaaatacac gatgcgagca
     9661 atcaaatttc ataacatcac catgagtttg gtccgaagca tgagtgttta caatgtttga
     9721 ataccttata cagttcttat acatacttta taaattattt cccaagctgt tttgatacac
     9781 acattaacag atactctata gaaggaaaag ttatccactt atgcacattt atagttttta
     9841 gaattgtgga taattagaaa ttacacacaa agttatacta tttttagcaa catattcaca
     9901 ggtatttgac atatagagaa ctgaaaaagt ataattgtgt ggataagtcg tccaactcat
     9961 gattttataa ggatttattt attgatattt acataaaaat actgtgcata actactaagc
    10021 aggataaagt tatccaccga ttgttattaa cttgtggata attattaaca tggtgtgttt
    10081 agaagttatc cacggctgtt atttttgtgt ataacttaaa aatttaagaa agatggagta
    10141 aatttatgtc ggaaaaagaa atttgggaaa aagtgcttga aattgctcaa gaaaaattat
    10201 cagctgtaag ttactcaact ttcctaaaag atactgagct ttacacgatc aaagatggtg
    10261 aagctatcgt attatcgagt attcctttta atgcaaattg gttaaatcaa caatatgctg
    10321 aaattatcca agcaatctta tttgatgttg taggctatga agtaaaacct cactttatta
    10381 ctactgaaga attagcaaat tatagtaata atgaaactgc tactccaaaa gaaacaacaa
    10441 aaccttctac tgaaacaact gaggataatc atgtgcttgg tagagagcaa ttcaatgccc
    10501 ataacacatt tgacactttt gtaatcggac ctggtaaccg ctttccacat gcagcaagtt
    10561 tagctgtggc cgaagcacca gccaaagcgt acaatccatt atttatctat ggaggtgttg
    10621 gtttaggaaa aacccattta atgcatgcca ttggtcatca tgttttagat aacaatccag
    10681 atgccaaagt gatttacaca tcaagtgaaa aattcacaaa tgaatttatt aaatcaattc
    10741 gtgataacga aggtgaagct ttcagagaaa gatatcgtaa tatcgacgtc ttattaatcg
    10801 atgatattca gttcatacaa aataaagtac aaacacaaga agaatttttc tatactttta
    10861 atgaattgca tcagaataac aagcaaatag ttatttcgag tgatcgaccg ccaaaggaaa
    10921 ttgcacaatt agaagatcga ttacgttcac gctttgaatg ggggctaatt gttgatatta
    10981 cgccaccaga ttatgaaact cgaatggcaa ttttgcagaa gaaaattgaa gaagaaaaat
    11041 tagatattcc accagaagct ttaaattata tagcaaatca aattcaatct aatattcgtg
    11101 aattagaagg tgcattaacg cgtttacttg catattcaca attattagga aaaccaatta
    11161 caactgaatt aactgctgaa gctttaaaag atatcattca agcaccaaaa tctaaaaaga
    11221 ttaccatcca agatattcaa aaaattgtag gccagtacta taatgttaga attgaagatt
    11281 tcagtgcaaa aaaacgtaca aagtcaattg catatccgcg tcaaatagct atgtacttat
    11341 ctagagagct tacagatttc tcattaccta aaattggtga agaatttggt gggcgtgatc
    11401 atacgaccgt cattcatgct catgaaaaaa tatctaaaga tttaaaagaa gatcctattt
    11461 ttaaacaaga agtagagaat cttgaaaaag aaataagaaa tgtataagta ggaaactttg
    11521 ggaaatgtaa tctgttatat aacagcacta ataataacta tcatttttta catttctata
    11581 tgctaatgtg gcaagatgag caaaactcat tttgtggata atgtttaaaa gtcatacaca
    11641 ccatacacaa gttatcaaca tgtgtataac ttcgccaaat ctatgttttt aagacttatc
    11701 caccaatcca cagcacctac tactattact aagaacttaa aacctatata attatatata
    11761 aacgactgga aggagtttta attaatgatg gaattcacta ttaaaagaga ttattttatt
    11821 acacaattaa atgacacatt aaaagctatt tcaccaagaa caacattacc tatattaact
    11881 ggtatcaaaa tcgatgcgaa agaacatgaa gttatattaa ctggttcaga ctctgaaatt
    11941 tcaatagaaa tcactattcc taaaactgta gatggcgaag atattgtgaa tatttcagaa
    12001 acaggctcag tagtacttcc tggacgattc tttgttgata ttataaaaaa attacctggt
    12061 aaagatgtta aattatctac aaatgaacaa ttccagacat taattacatc aggtcattct
    12121 gaatttaatt taagtggctt agatccagat caatatcctt tattacctca agtttctaga
    12181 gatgacgcaa ttcaattgtc ggtaaaagtg cttaaaaacg taattgcaca aacgaatttt
    12241 gcggtgtcca cctcagaaac acgcccagta ctaactggtg tgaactggct tatacaagaa
    12301 aatgaattaa tatgcacagc gactgactca caccgcttgg ctgtaagaaa gttgcagtta
    12361 gaagatgttt ctgaaaacaa aaatgtcatc attccaggta aggctttagc tgaattaaat
    12421 aaaattatgt ctgacaatga agaagacatt gatatcttct ttgcttcaaa ccaagtttta
    12481 tttaaagttg gaaatgtgaa ctttatttct cgattattag aaggacatta tcctgataca
    12541 acacgtttat tccctgaaaa ctatgaaatt aaattaagta tagacaatgg ggagttttat
    12601 catgcgattg atcgtgcatc tttattagca cgtgaaggtg gtaataacgt tattaaatta
    12661 agtacaggtg atgacgttgt tgaattatct tctacatcac cagaaattgg tactgtaaaa
    12721 gaagaagttg atgcaaacga tgttgaaggt ggtagcctga aaatttcatt caactctaaa
    12781 tatatgatgg atgctttaaa agcaatcgat aatgatgagg ttgaagttga attcttcggt
    12841 acaatgaaac catttattct aaaaccaaaa ggtgacgact cggtaacgca attaatttta
    12901 ccaatcagaa cttactaaat ataaataaag gatgacg
//
LOCUS       DAKSQE010000075        12917 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.075, whole genome shotgun sequence.
ACCESSION   DAKSQE010000075 DAKSQE010000000
VERSION     DAKSQE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12917
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.075"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..148)
                     /locus_tag="O0V33_001844"
     CDS             complement(<1..148)
                     /locus_tag="O0V33_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976468.1"
                     /translation="MINETKYMRQQITNLIQIIDTIKYNTLMTDWNIQTHIYKFNQIV
                     TNELN"
     gene            197..934
                     /gene="sdpB"
                     /locus_tag="O0V33_001845"
     CDS             197..934
                     /gene="sdpB"
                     /locus_tag="O0V33_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HCX8976469.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGY
                     YEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYIKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
     gene            complement(959..1651)
                     /locus_tag="O0V33_001846"
     CDS             complement(959..1651)
                     /locus_tag="O0V33_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HCX8976470.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            2036..2833
                     /locus_tag="O0V33_001847"
     CDS             2036..2833
                     /locus_tag="O0V33_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCX8976471.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATHFPEHQLLAEEKSNEMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGFKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
     gene            2966..3913
                     /locus_tag="O0V33_001848"
     CDS             2966..3913
                     /locus_tag="O0V33_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCX8976472.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
     gene            4515..7469
                     /gene="fdhF"
                     /locus_tag="O0V33_001849"
     CDS             4515..7469
                     /gene="fdhF"
                     /locus_tag="O0V33_001849"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCX8976473.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTSIKEKHGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIEEWVEDFDEYYK
                     SLETFTMAFAEEATGIPEAELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGQIHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATYADVVLPASPSLEKDGTFTNTERRIQRLYQALKPLGDSKPDWKIFQAIANKLS
                     FDWNYKHPSEIMDEIARLTPLYAGVSYERLEGFNSLQWPVHPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEVYDIHVNNGRLLEHFHEGNMTYQTPMLKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRMEVITKRGKSPLNPNNFRVNKKRHPQYSVQVQKKWER
                     PDYVFPGNQVDK"
     gene            7469..7942
                     /locus_tag="O0V33_001850"
     CDS             7469..7942
                     /locus_tag="O0V33_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HCX8976474.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKTLDDAKLLDALNGAVRGRQVIINKFAVELNKDIYTGLLSNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTSVRSLLGVLKDDDMNRSLTYMLNMLKGMSRED"
     gene            8236..8469
                     /locus_tag="O0V33_001851"
     CDS             8236..8469
                     /locus_tag="O0V33_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976475.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            8491..9267
                     /locus_tag="O0V33_001852"
     CDS             8491..9267
                     /locus_tag="O0V33_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCX8976476.1"
                     /translation="MKKNFKLRISTLLLIVILVVFSVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAILGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
     gene            9876..11000
                     /locus_tag="O0V33_001853"
     CDS             9876..11000
                     /locus_tag="O0V33_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HCX8976477.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRPLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDAIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNAYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLVVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(11092..11970)
                     /locus_tag="O0V33_001854"
     CDS             complement(11092..11970)
                     /locus_tag="O0V33_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HCX8976478.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKD
                     ATALISLLSTNVDKDVIDESIDVTNTPKASTNATADLTIGLVLAVARRIVEGDQLSRT
                     TGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAVARRARAFDMDVLYTGPNRKEEKERE
                     IGAKYVDLDTLLKNADFITINAAYNPKMHHLIDTEQFKMMKSTAYLINASRGPIVHEQ
                     ALVQALKNNEIEGAALDVYEFEPDITDDLKSLNNVVLTPHIGNATFEARDMMSKIVAN
                     AAISAVQGEKPQFVVN"
     gene            complement(12329..12820)
                     /locus_tag="O0V33_001855"
     CDS             complement(12329..12820)
                     /locus_tag="O0V33_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCX8976479.1"
                     /translation="MKKLVTATTLTAGIGTALVGHAQHADAAENYTNYNYNTTQTTTT
                     TTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTVNHT
                     PSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGNYNY
                     IHI"
BASE COUNT         4491 a         1836 c         2427 g         4163 t
ORIGIN      
        1 tattcaattc attagtaact atttggttaa atttataaat atgcgtttga atgttccaat
       61 ctgtcattaa agtattatat ttaatcgtat caatgatttg aatcaaatta gtaatttgtt
      121 gtctcatata cttagtttcg tttatcatgt agaagtacct ccaaaaatca attattaaac
      181 aggaaaggat ataacagtgg aaaatgaaaa aaagaaatac acgtttaaag atatagcatg
      241 gagagatttg cttataattc caattatagg tgttatattt tatgcactca caattgcagc
      301 ttacatagga ccaattttgt ttggttatta tgaaatcgat agtaatctga tatttgtagc
      361 gactatcaca caagctttag catatacgat tgggattggc gcattttatt tatttcattt
      421 aaaagtgatg ccatcaaggc ttcgagctgg ttttgcgtat atcaaaaaat attggcttag
      481 attgtcaata acgtatatta ttgctctcat cttaatttat acatatgaat atatgacaca
      541 gtttctacct aaacatttgc aatatagtga aacagccaat gaacttgagc taaataaaat
      601 gtttgaagtg cctgcatttt tacctgtagc attcttatta attgtgattg taggtccaat
      661 agttgaagaa atcgtattta gacatatttt aattggagaa ttaggtaaga agtttaattt
      721 tatagctatg agtattgttt cggtgttctt atttgcattt attcatgtga ctgatgcaaa
      781 gtcgccgttt gagtttggtc catatctcat actatccatc atattagttt tcacttatat
      841 aaaatctgga agaaacctag gttcaacaat tgcattacat atagcaaata attttgtttc
      901 tttcgttata tcagtaataa gtatttatgc gtagataaaa ccccgtcgac tccatagttt
      961 aggaatcgat ggggtttaaa aattgatgat agttatcaat ggatatgtct ctggtttgaa
     1021 tcgtcttacc gaatgaatac gaaatatcta taatattttc aatttcgagt tcggcagttt
     1081 tgtcttgagc aatgtcataa atgactgcat gccaatgtcc atgtttacac aaaaggccaa
     1141 taaatataac gttttcggct ttaatattat catcaaccct atgatctact aacatcacat
     1201 ttcgttctgt acaataaatt aaaatgtcgg aaaacaacat tgggagtgtc atatgttgtg
     1261 tgtcatcaaa tttaatataa aagtccatgc gcttaagcaa ctttcttaag cgtgtttgtg
     1321 gaagacttaa acattgtttt ataattgcgt tgatttcgct tttatatggt aatgtcgagt
     1381 acgactgact ttcattaagc actaaaaata aggccgataa ttgtgtttca gataagttta
     1441 atgcaatttt agattgtgct tgttgtattt ggtaaccacc atttttccca taatgcgcat
     1501 aaattttaac accttgattt tctaaatcat caatatctct taaaattgtg cgtttggata
     1561 cgttgcaata tttagctaat tctaaagcgg tcattttttt attttgttga attgcagtaa
     1621 ttattaaatt ttgcctttca gctttattca tgacagccac ctcctaaaag atgatatatt
     1681 acacaaagta taacataaga gtgacaatga ggacattttc aatatctttt taaaaaaatc
     1741 ggaaaaattt tgtggaaaac gcttacattt taaaaaagat tgtgctatga tattacttgt
     1801 aacaggggga gcgattaaac aaaggggtag agctatataa cagatagctt ttaatcgttc
     1861 aagttacatc acaacatctt caatatttat tacttacttt cctttctatt tgtcggctag
     1921 cacgtgacta gccgattttt ttatgcataa aatgaagaag tcttcagttg tattgttgat
     1981 tcttttaata tagggtaata ataaaatgat aatgatatga gaagtggtgg acgaaatgac
     2041 agataaaaca ttacagcaaa tagataagtt aatatgttcg tggttgaaac aaatagacaa
     2101 tgtgattcca caattaatta tggagatgac tacagaaacc aagcgtcatc gatttgattt
     2161 agttacaaat gtagataaac agattcaaca acaatttcaa caatttttag caacacattt
     2221 tccggaacac caactattag cggaagaaaa aagtaatgaa atgattacga atgaaattaa
     2281 tcatttatgg attatggatc ccattgatgg aactgccaat ttagtaaaac agcaagaaga
     2341 ttattgtatt atattggcgt atttttatga aggtaaacca atgttatcgt acgtatatga
     2401 ttatccgcat aaaaagcttt ataaagcaat acgaggagaa ggtgcttttt gtaatggatt
     2461 taagatggaa gaaccaccat cgttaaaatt ggaagacgct attatatcgt ttaatgcaca
     2521 agtgatgaat ctggatacgg tgcaagattt atttgacgcc tcatttagtt atcgtttagt
     2581 tggtgcttgc ggattagatt ctatgcgagt ggcaaaaggt caatttgggg cacatattaa
     2641 tacaaatcct aaaccttggg acattgctgc acagttttta tttgcagaac tattgaatct
     2701 aaaaatgaca actttagatg gtaaggcaat tgatcattta aagggtgcac cttttattat
     2761 aagtaataaa gcatgtcatg aaacggtact taaaatttta aatgcaaatg gtggttatca
     2821 aaaatacaga tagatattct cgagagataa aaaatgataa aaaatagtta tacgtctaca
     2881 gaagtatagt aaaaaaatta ttttactata taataaatag agaaaccaga caaagtatgt
     2941 aaggaatagt aaaggagtgg tgggattgaa acgttcgtct aaaagtaaaa tgagcctacc
     3001 gaaaaaaata tttttatggg tattcggtat tttagtaatc ttagcgattg tagctgttgt
     3061 gtatgtagct gctaaaattt ttattactgg taataagata cacaatccgt tagatcgtaa
     3121 tcattcagaa ttaagagata aaaaagtgag tttaaacgat ggtgatccat tcacaatcgc
     3181 tttatttggt gtcgattcag atgctgatcg taagaaaaag ggtggcggag agcgtagtga
     3241 tagtattatg attttatcta tcaaccctaa aacaaagaaa actgaaatag taagtatacc
     3301 gcgtgataca agagcggaaa ttgttggccg tggtactact gaaaaaattg cacatgccta
     3361 tgcatatggt ggaccaaata tggctgttaa atcacttgaa aaattgatga atgtgccaat
     3421 tgatcattat gcgacaatcg atatggatgg tttgcataat atgatagata gtatcggcgg
     3481 tgttgatgta gtaagtaatg atacattcac tgttgatggt gttcgtttta caaaaggaca
     3541 gcaaacacat gtaaatggtg atcaagcatt gaagtttatt agaagtcgta aagaagaagg
     3601 tgctggtggt gactttggtc gacaacaacg tcagcaaatt gtacttgaag cgatggctaa
     3661 taagattgca agcccatcat cgattacaca ttttaatagt ctaatgaatg aaattcaaaa
     3721 taatgttaaa acagatttaa cattaggtga tttgaatacg attagaagca attataaaga
     3781 tgctaatgac acaattaata aacatcaatt gagtggtcaa ggcggcattc aaagtgacgg
     3841 attgtactac ttcattccta gtgagcagtc aaaagctgaa agtactaagt tattaaaaga
     3901 taatctagag tagtaaacta agggaaaata agttatcaaa tttgtacaca aattaattag
     3961 atagaatgag ctgtaaaaag aataacgaat agaattaata gcccgtaaat agaaggtgat
     4021 aacactttgt atttatgggc tattttattt aatgcctgat ttgattttta caaattattc
     4081 cattacaaat ttaatttaaa gataaatctt accttataaa tggcagtaga tatttatcaa
     4141 acttgggtta ttaattttca gaaaaagggt atttataaat agtatagcga tttttaggtg
     4201 aggtgattat taagtattgt cattaataag gttgtgaaag atatggcaaa aagcgtgaca
     4261 cagataattg gaaaactaat aatgataatt attttcaatt aaacaaaaag atgatttaat
     4321 ttcgcgcaaa atatttcaaa taaaaatgat tgcaaaaata aaaaatacaa aaatacgctt
     4381 aacattatac acttaaagta atacaattat attgattcta tctgaaagat gtgtggggca
     4441 tctttatttt aggtggaaat taggcaattt attaaaagtc atttacggaa aaatatatag
     4501 atggggtgaa tagtatgcaa gaacatttgg tggtcacgct tgatggtaaa gattatcttg
     4561 tagaaccagg tacaaattta cttgaattca ttaaatcgca agatactttt gtgccttcaa
     4621 tttgttataa cgaatcaatg ggaccgatac aaacgtgtga tacatgtaca gttgagattg
     4681 atggtaaaat tgaacgttca tgtagtacag tgattgatcg tccaatgaca gtaaatacag
     4741 tgaataatga cgtgaaagat gctcaaaaag aagcgcttga tcgaatttta gaaaagcata
     4801 tgctgtattg tacagtatgt gattataata atggtgattg tgaaattcat aatacgatgg
     4861 atgcatgggg gcttcagcat caaacgtatg aatataaaga gaagccatat gaaaaagatt
     4921 atggcccatt ttatcgctat gatccaaatc aatgtatctt atgtggccgt tgtgtagaag
     4981 cgtgtcaaga tattgaagta aatgaaacaa ttagaattga ttgggatcgt gagcatccac
     5041 gtgtcatttg ggataatgat gtaccgatta atgaatcgtc atgtgtatct tgtggtcaat
     5101 gcgcaactgt atgcccatgt aatgcgatga tggaagttaa catggaaggt aatgcaggtt
     5161 atatgactga tacggaacct ggttcattag cggcaatgat tgatttaact aaaaaagcag
     5221 aacctggtta tggtccacta tttgcaatct cagattcaga agcagaaatg cgtaaagagc
     5281 gtattaagaa aactaaaaca gtgtgtacat attgtggtgt gggctgttca tttgaagttt
     5341 ggactaaaga tagagaaatt ttaaaagtac aaccatcaca tgattctcca gcaaataaaa
     5401 ttgcaacttg tgttaaaggt aagttttcat ggggacatat taattcagat caacgcttaa
     5461 cgaaaccatt agtaaggaaa aatggtgagt tccatgaagt agaatgggat gaagcattaa
     5521 atgtcattgc agataatttt acatctatta aagaaaagca tggtccagat gcattatcat
     5581 tcatttcttc ttctaaagcg acgaatgaag aatcgtattt aatgcaaaaa ttagcaagac
     5641 aagttattgg cacaaataac gttgataact gttcaagata ttgccaagca cctgcaacaa
     5701 aaggcttatt tagaacggtt ggacacggcg gtgactcagg tagtattgaa gatttagaaa
     5761 aagcagcaat gtctgtattg attggtacga atacagctga agcacatccg gttattgcat
     5821 cacgcatgaa gcgtgctcaa aaattatttg gacaaaaaat acatgtgttt gatattagaa
     5881 aacatgaaat ggctgaacgc gcagatcgtt tttatcaacc gaaaccaggt acagatttgg
     5941 cttggttaag cgcagtgacg aaatatatca ttgatcatga cttacacgat aaagccttta
     6001 tcgaagagtg ggttgaagat tttgatgaat attacaagtc attagaaaca tttacaatgg
     6061 cattcgctga agaggctact ggtattccag aagcagagtt gattaagttc gctgaagaat
     6121 gtgccaaagc tgagtccgtt gtcatttgtt gggcaatggg tatcacacaa caagacattg
     6181 gaagtgattc aagtacagca atttcaaact tattattagt tactggaaat tatcgtcgac
     6241 ctggtacagg cgcgtatcca ttacgtggac ataataatgt tcaaggttgt agtgatatgg
     6301 gtagtatgcc tgataaaatt actggctatc aaagtattga agcggacgat attcgcgcta
     6361 aattcgaaaa agaatatggc gttaaattga atccgaaagc tggtaaagat aatcatgaaa
     6421 tggttgaagg tatccatgat gggcaaattc actcattata tctatatgga gaagatactg
     6481 gtattgtaga ttcaaatatt aattttgtac aagctgcttt tgaaaaatta gatttcatgg
     6541 tagtccaaga tgaattttta acattcacag caacatatgc agatgttgtc ttgccagcaa
     6601 gtccttcatt agaaaaagat ggtactttta caaatacaga gcgacgtatt caacgtttat
     6661 accaagcatt gaaaccatta ggtgattcta aacctgactg gaaaatattc caagcaattg
     6721 ctaataaatt aagtttcgat tggaattaca aacatccaag cgaaattatg gatgaaattg
     6781 ctagactaac accattatat gctggcgtaa gctacgaacg tttagaagga tttaatagtt
     6841 tacaatggcc tgtgcatcca gatggtacag atgaaccaat actttattta gaaggattta
     6901 atttcgataa tggtaaagca aaattattcc cattatcatt tgataattac tttaagcaag
     6961 atgaagttta tgatattcat gtaaacaatg gtcgattgtt agagcacttc cacgaaggta
     7021 acatgacata tcaaacacca atgcttaaat acaaagtgcc acgtgcattt gttgaaattt
     7081 ctccagaact tgctgaagat agaggcattc atgaaggtgc agaagttaag ttgatatctg
     7141 aaacaggaga agcggtatta caagttcacg tgacagatcg tgttaaaggt aaagaaatct
     7201 atattccatt aaataatgat gcgatggaaa atggagatct aggcgcaatt aacttattaa
     7261 ctaatagtga tgttgatcaa tacacggata caccatctta taaacgtaca agctgtcgta
     7321 tggaggtaat tacgaaacgt ggtaagtcac cgttgaaccc taataatttc cgtgtcaata
     7381 aaaaacgtca tccgcagtac agtgttcaag tacagaaaaa atgggaacgt ccagattatg
     7441 ttttcccggg aaatcaggtg gataaataat ggctgagaga atatcttcta aaattagacg
     7501 cttagaaaaa tcagaagaac aaattaagtt agaaagttta aatgaagtta cagaggcaat
     7561 tgctgcaaac aaagatagca ttttaaaagc aatcaagctc attaaaacat tagatgatgc
     7621 aaaactatta gatgctttaa atggcgcagt tagaggacgt caagtgatta ttaataaatt
     7681 tgctgttgag ctcaacaaag atatttatac agggctatta tctaatatgg cttcaatggt
     7741 atttttatta ggcgaattga atgtatcaga tttaagtgac ttccttaata aagttaacaa
     7801 aggtttacac gttgccaatc aagcgagtcc taatgcgaag acatctgtaa gaagtctatt
     7861 aggcgtttta aaagatgacg atatgaaccg tagtttaaca tatatgctta atatgttaaa
     7921 aggtatgtct cgagaagatt aatatgagtc tataataaat cggattgatg taaaagtttt
     7981 ttacttttca tctttccgat tttttgtttt tgaatataac agtgtttctg catgtcggtg
     8041 taggggcact gttttaagtt atattgaaat attatttgta tccgagtctt catcaaactt
     8101 caaacaatat ttttgagaat cgagaaaatc atgtgctatt gataatcaac catcgacagg
     8161 gctatgaaac atcatgtatt attatgatta tgtaagttat ttaaataaaa agggtgagtg
     8221 catattaatt cgagaatgtt aaaaacgtca gctgtaattt ctaatacatt attagtcata
     8281 ggtttagtct gtctatttat gatgaaatta gtgttagcta ttactttttt tgctgtttca
     8341 ttatcaatta gtctagttgt ctttaatatg atgttcagag atagaacaac gatgaaagta
     8401 atcgttaacg cttctttttt aatcgtaata ttagcgattg tagtggcata ttttttattg
     8461 tctaagtaag aaaaatgagg ttttgtctat atgaagaaga atttcaagtt acgtatttca
     8521 acgctactat tgatagttat tttagttgtt ttttctgtat tgctcatcgt gaatgaaact
     8581 aaattgttta aaaatgatgt gaattactct tttgatgagg ctgtttcaat gcaacaaggg
     8641 aaaggtattg tacagacaaa agaagaggat ggtaaatttg ttgaagcaaa taataatgaa
     8701 attgctaaag caatgactat ttcacataaa gacaatgata tgaagtatat ggatatcaca
     8761 gaaaaagtgc caatgtcgga atctgaagtt aatcaattgc taaaaggtaa ggggatttta
     8821 gaaaatcgag ggaaagtttt tctagaagct caagaaaaat atgaggttaa tgtcatttat
     8881 cttgttagcc atgcattagt agaaacaggt aacggcaaat cagaattagc aaaaggcatt
     8941 aaagatggaa aaaaacgcta ttacaacttt tttggtatag gagcattcga tagtagtgct
     9001 gttcgtagtg ggaaaagtta tgctgaaaag gaacaatgga catcaccaga taaggcgata
     9061 cttggtggtg caaagttcat tcgtaatgaa tattttgaaa acaatcaact gaatttatat
     9121 caaatgcgat ggaatccaga aaatcctgcg caacatcaat atgcgagtga cattcgctgg
     9181 gcagataaaa ttgccaaatt aatggataaa tcctataagc agtttggtat aaagaaagat
     9241 gatattagac aaacatatta taaataagac atcggtgctt aaaggggctg gaacaattta
     9301 ttgtttcgag ctcctttagc gcattctgag tgtgttagtt aaatggattt taacctaaca
     9361 taaaacgcgg gagtgggaca gaaatgatat ttttacaaaa tttatttcgt tgtcccaccc
     9421 caacttgcat tgtctgtaga aattgggaat ccaatttctc tttgttgggg ccccgccaac
     9481 ttgcattgtc tgtagaaatt gggaatccaa tttctctttg ttgggaccca caccccaact
     9541 cgcattgcct gtagaatttc ttttcgaaat tctctttgtt ggggcccctg actagagttg
     9601 aaaaaagctt gttgcaagcg cattttcgtt cagtcaacta ctgccaatat aatattatag
     9661 accctaggac attgatttat gtcccaagct cccacatcat tgttacaaat gtacattatg
     9721 taaatgaata ttgctgtcta aatgtgcatg taatatacaa tggtgcagat aatacactta
     9781 agtctttaaa aatgaaacgt tagttccaag agtcattttt aaacaatagt gcatgtgata
     9841 aaatagaaaa gaatgaaaaa tatagaggtg acaatatgaa gatagcaatt ataggtgcag
     9901 gcatcggtgg attaacagct gctgcactat tacaagaaca aggtcatact attaaagtct
     9961 ttgaaaaaaa tgagtcagtt aaagaaattg gcgctgggat tggtatcgga gataatgtgc
    10021 ttaaaaaact aggtaatcat gacttagcta aaggtattaa aaatgctggg caaatcttat
    10081 ctacaatgac agtgttagat gacaaagatc gcccgttaac tacagttaaa ttaaaaagta
    10141 atacattgaa tgtgacgtta ccacgccaaa ctttaattga cattattaaa tcttatgtaa
    10201 aagatgatgc aatatttaca aatcatgaag tcacgcatat agataatgag acagataaag
    10261 ttaccataca tttcgcggaa caagagagtg aagcatttga tttatgtatt ggtgctgatg
    10321 gaattcattc taaagtgaga caatctgtaa atgctgacag taaagtatta tatcaagggt
    10381 atacatgctt tagaggttta attgatgata ttgatttaaa acatccggat tgtgcaaaag
    10441 aatactgggg aagaaaagga agagtaggta ttgttccgtt attaaataat caagcatact
    10501 ggttcattac aattaactcg aaggaaaaca atcataaata tagttcgttt ggtaaacctc
    10561 atttgcaagc atactttaat cactatccaa atgaagttag agagatctta gacaaacaaa
    10621 gtgaaacagg tatcttattg cataatattt atgatttgaa accactcaaa tcttttgttt
    10681 atggtcgtac tattttacta ggagatgcag cacatgcgac aacgcctaat atggggcaag
    10741 gtgctggaca agcaatggaa gatgctatcg tattagtaaa ttgttttaat gcatacgact
    10801 ttgaaaaagc attacagcgt tatgataaaa tacgtgtcaa acatactgca aaagtaatta
    10861 aacgttctag aaaaatcggt aaaattgccc aatatcgtag tcgtttagtt gttgcagtta
    10921 gaaatcgtat tatgaaaatg atgccaaatg cattagcagc tggacagact aaattcttat
    10981 ataaatcgaa agaaaagtaa tacaacaata tgaaaacccc cgtatgttga aattagagct
    11041 caacatatgg ggggtcttgt ttttataatg tcattataat aaattcaatt attagttaac
    11101 gacaaattgt ggtttctcac cttgaacggc actaattgca gcattagcaa caattttaga
    11161 catcatgtca cgtgcttcaa atgtagcatt accaatatgc ggtgttaata ctacattatt
    11221 aagtgatttt aagtcatcgg taatgtctgg ttcgaattca tatacatcaa gtgcagcacc
    11281 ttcaatttca ttatttttca atgcttgcac tagtgcttgt tcgtgcacga ttggaccacg
    11341 agaggcattg attaaatacg ccgtagattt catcatttta aattgttctg tatcaattaa
    11401 atgatgcatt ttaggattgt aagcagcgtt gatagtgata aaatctgcat tctttaatag
    11461 tgtatctaaa tctacatatt ttgcaccgat ttctcgttct ttttcttctt tgcgattagg
    11521 tcctgtgtat agtacatcca tgtcaaatgc tcttgcacga cgcgctactg cactaccaat
    11581 ttcacctaaa ccgataatgc cgattgtttt cccagatact tctctacctc tgaaaaataa
    11641 aggtgcccat ccgtcaaatc cagttgtacg tgataattgg tcgccttcaa caatacgacg
    11701 cgctactgca agtactaatc caattgttaa atcagcagtc gcgtttgttg atgctttagg
    11761 tgtgtttgta acatctatac tttcatcgat aacatcttta tcaacgtttg tagataataa
    11821 actaattaag gcagtcgcat ctttaacacc tttaattaaa gtgtctttat cgactaatcc
    11881 tttaccttca tacatttcaa cttcaaaatg ttcttgtaaa agttttaaac ctacttctgg
    11941 tattgcacca gcaacataaa ctttttccat aaaagatcac tccttttatc ttagtatagt
    12001 agaagattag acagtataca actatgtcat gatgtcttgt gtatcaatga tgtaagcgtg
    12061 tacttttgat ggaggtgata taacttagtc actgtagaac tatgaatatt gtaatgtgga
    12121 aaactggatc aattaaatta gataacgtag ttttaaagtt aatagtattg aaaaaattaa
    12181 tattatgaat atgggaggaa atatatataa gtaggtggca atgaaaaata gcaaaaaaag
    12241 agcttctcct ataaaggaaa gctcaaagtt tttgatgaca tatgtactag aattaagttt
    12301 caagacaata tgtatcatcg tgtttaaatt aaatatggat gtagttatag ttacctgctt
    12361 cacttgcaga aatagttctt gagcttactg agaaaggtcc gccactataa ttcatttctg
    12421 aaattgtaac tgaaccatca ctgtttacac tttctacata tgcaacgtga ccaaatggtc
    12481 cttcagaaga ttgtaggata gcgcctttag aaggtgtatg gtttactgtg aatccagcac
    12541 cttgtgcagc agcagcccaa ttattagcat ttccccaagt agatccgatt tctccgccta
    12601 ctttatcata tacataccaa gtacattgtc ctgcagtgta taagttacca gaatgtgaaa
    12661 ttgatgatgt agttgtcgta gttgttgtag tcgttgtagt ttgagtcgta ttatagttat
    12721 aatttgtata attttcagca gcgtctgcat gttgtgcatg acctactaat gctgtaccaa
    12781 ttcctgctgt taacgtagtt gctgttacta attttttcat gaataaagtc ctccaaagtt
    12841 ctatatcttt ttttataaat aaaacgtagc gactgtttta ttctcacatc tcgaattgat
    12901 gacaatagtt actttaa
//
LOCUS       DAKSQE010000076        12732 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.076, whole genome shotgun sequence.
ACCESSION   DAKSQE010000076 DAKSQE010000000
VERSION     DAKSQE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12732
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.076"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(169..552)
                     /locus_tag="O0V33_001856"
     CDS             complement(169..552)
                     /locus_tag="O0V33_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCX8976480.1"
                     /translation="MKERGHIEQLWREEKYHVLLHSQQSYQMIRNALKKNLSLHQVQQ
                     MIDDALLIEPSIGSVCNAFDHMWGYFKKCANEEERQQSKLLKADFINGKIDTQTLLGF
                     LAELANKYDVQYLLQSRVLNTKRKR"
     gene            735..2177
                     /locus_tag="O0V33_001857"
     CDS             735..2177
                     /locus_tag="O0V33_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HCX8976481.1"
                     /translation="MNYKQSAEDILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPV
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIVAITVTLILSKRKANKEVVE"
     gene            complement(2263..3207)
                     /locus_tag="O0V33_001858"
     CDS             complement(2263..3207)
                     /locus_tag="O0V33_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCX8976482.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(3253..3876)
                     /locus_tag="O0V33_001859"
     CDS             complement(3253..3876)
                     /locus_tag="O0V33_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCX8976483.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGIPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIVFNGPLRIMAENRLHKSNLDSLT"
     gene            4037..5314
                     /locus_tag="O0V33_001860"
     CDS             4037..5314
                     /locus_tag="O0V33_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCX8976484.1"
                     /translation="MNIFKNKLLWIAPIATMIILVIFSLAFYPAYNPKPKDLPIGILN
                     EDKGTTIQDKNVNIGKKLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDK
                     DFSKDAMSKTQKVVMDSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTI
                     VSEGSSLQGSQIASAVLTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVD
                     NEKLNKVKDHQAGGNAPFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQM
                     IFAVVAAFAGSFVYIYFMQGIQGFDFDHPNRIAIFVALAILGFVGLILGVMVWLGMKS
                     IPIFFILMFFSMQLVTLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNST
                     MWMFIGFMIFGAVSSLISAIVRKHSTKRTEVPS"
     gene            5520..5951
                     /locus_tag="O0V33_001861"
     CDS             5520..5951
                     /locus_tag="O0V33_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HCX8976485.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            6051..6167
                     /locus_tag="O0V33_001862"
     CDS             6051..6167
                     /locus_tag="O0V33_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976486.1"
                     /translation="MANYAIYFNNININSIMSLNDNNYQFERVNERNEYRES"
     gene            6178..7212
                     /locus_tag="O0V33_001863"
     CDS             6178..7212
                     /locus_tag="O0V33_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCX8976487.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKSERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDTHAVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            7248..7649
                     /locus_tag="O0V33_001864"
     CDS             7248..7649
                     /locus_tag="O0V33_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCX8976488.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDKDRLIGMGRVIGDGGTVFQIVDIAVLKSYQGQGYGSQIMEHIMKYIKNVAVESTYV
                     SLIADYPADKLYAKFGFMPTEPDSGGMYIKY"
     gene            7814..7990
                     /locus_tag="O0V33_001865"
     CDS             7814..7990
                     /locus_tag="O0V33_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976489.1"
                     /translation="MKIDTLRQAIIYYFVALVLSALMIYLFLTNIERVILYSCVFTVV
                     YWAIYAFDVYRKRN"
     gene            complement(8215..9219)
                     /locus_tag="O0V33_001866"
     CDS             complement(8215..9219)
                     /locus_tag="O0V33_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCX8976490.1"
                     /translation="MTETFKAFVIDKDESGKVTPTFKQLSTTDLPKGDVLIKVHYSGI
                     NYKDALATQNHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIVKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSNQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLKPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK
                     "
     gene            9409..9924
                     /locus_tag="O0V33_001867"
     CDS             9409..9924
                     /locus_tag="O0V33_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCX8976491.1"
                     /translation="MAGIIKEISKQDASELVELATRTFYDTFGSYYDDNDFDQFFKDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDF
                     QGGGRGSQLIALAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            10240..10869
                     /locus_tag="O0V33_001868"
     CDS             10240..10869
                     /locus_tag="O0V33_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976492.1"
                     /translation="MKKLVTGLLALSLFLAACGQDSDQQKDSNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQSNSGQTTN
                     NQKSSYVAPYYGQNAAPVARQIYPFNGNKSQALQQLPNFQTALNAANNEANKFGNGHK
                     VYNDYSIEEHNGNYKYVFSFKDPNVNGKYSIVTVDYTGQAMVTDPNYQQ"
     gene            complement(11023..12696)
                     /locus_tag="O0V33_001869"
     CDS             complement(11023..12696)
                     /locus_tag="O0V33_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCX8976493.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHAVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPLATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNWPDSWLYSTKFMLPKWLYKIAKQRADNKQLQNLG
                     LYTKQPNVLKDHIVFIGDHYQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPK
                     SEKADELINSARVVLVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKRNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDETMLPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQQKEQPLESISQLILSKMTSNK"
BASE COUNT         4350 a         1816 c         2267 g         4299 t
ORIGIN      
        1 gctgtaacta attaaatgtg tatggtatct tctaacatca tgaaacgcat tttaatatag
       61 atatgtacat ttaaaccttg agagatagat tatagtaaac tttagtacaa agtatgaatt
      121 gctgaactcc aatgactact atttgtccgt tacaactaaa gtttcaatct atctctttct
      181 ttttgtgttt aaaacacgac tttgtagtaa atattgaaca tcatatttat tagcaagttc
      241 tgctaaaaaa cctaataaag tttgggtgtc gattttacca ttaataaaat cagctttaag
      301 tagttttgat tgttgtcttt cttcttcatt cgcacatttt ttaaaatatc cccacatatg
      361 atcaaaagca ttacatacac ttcctatact cggttcaatt aacaaagcat catcaatcat
      421 ttgttggact tgatgaagtg ataaattctt ttttaaagca ttccttatca tttggtaaga
      481 ctgttgactg tgtaacaata catgatattt ttcttcacgc caaagctgtt cgatatggcc
      541 acgttctttc ataaaattac ctccgataat taatgttaat atacttgcat gtagtatagc
      601 aataaaaatg aatgcgctat caaaagtgat gtaattgttt atataaaatg ataatagagt
      661 gttgcaaata tttttggaac cggttacaat aaagtggaat aaatggaacc ggttctaatt
      721 aggaggctaa atatatgaat tataagcaat ccgcagaaga tattttgaac gcgataggcg
      781 gagaagagaa tttagatgca atggcgcatt gtgcaacgag actacgatta gttttaaatg
      841 atgaaagttt agtaaatgaa gaggcgctaa acaatatgga tgtagttaaa gggacatttt
      901 ctactggggg acaataccaa attattattg ggtctggtac agtcaataaa gtatttagtg
      961 aactggaaaa attaactgga aaagaagcat caaccacttc ggaagtcaaa gcacaatctg
     1021 ctaaaaatat gaatccggta cagcgatttg taaaaatgct ttcagatatc tttgttccga
     1081 ttataccagc catcgttgct ggtggtttat taatggggtt aaataacatt ttgactgcga
     1141 aagatttatt cttttcaggt aaatcattga tagatgtata tagtcaattt gctggtttag
     1201 ctgaaatgat aaatgttttt gcgaatgcac catttacatt attaccaatt ttaattggat
     1261 ttagtgcagc aaaacgcttt ggtggcaatc cgtttttagg tgctgcatta ggtatgatat
     1321 tggttcatcc atcgctaatg agcgcatacg atttcccaaa agcagttgaa gcaggtaagg
     1381 ctattccata ttgggatgta tttggtttgc atattaatca agtaggttat caaggacaag
     1441 tcttacctat gcttgtagca gcttatatct tagcctcaat tgaaaaaggg ttacgcaaag
     1501 ttattccaac ggtgttagat aatttgttaa caccattgtt atctattttt ataacagcat
     1561 ttctaacatt ttcatttgta ggtccaatca ctcgacaatt aggttactgg ttatcagatg
     1621 gtttaacatg gctttatgaa tttggtggtg caattggtgg attaattttc ggattattgt
     1681 atgctccgat tgttattaca ggtatgcatc atagctttat agctgtagaa acgacattaa
     1741 ttgcagatgc cactaaaaca ggtggatcat ttatattccc aattgcgaca atgtctaatg
     1801 ttgcacaagg tggtgcagca attgcagcgt tctttattat taaacaaaat aaaaagttaa
     1861 aaggtgtggc atctgccgca ggtatttcag cattacttgg tattacagaa ccggctatgt
     1921 ttggtgttaa cttaaaacta agatatccat ttattggcgc tatcgttgga tcaggtattg
     1981 gttcagcata tattgctttc ttcaaggtta aagcaatcgc attaggaact gctggattgc
     2041 ctggatttat ttcaatcaat ccagtacatg caggatggtt acactacttt gttggtatga
     2101 caatatcatt catcgttgct ataacagtta ctttaatttt atcgaaaaga aaagcaaata
     2161 aagaagttgt agaataaaaa aagaggtatg acagtaatca aagtatcagt cattgcaaat
     2221 gcaataggat gaaactttaa gctgtcatac cttttaattt agttataatt tctgttttcg
     2281 ccataagaaa atactaacaa ttacgacaag aattaatgaa atacctacca caataatcca
     2341 agatatataa ctatggtcat caattggcaa cggcacattc ataccgaaga aactaaatac
     2401 gagcgttggt aacgttaata gtaccgtgaa aagtgttaat gtcttcataa tcgtattcat
     2461 atcattagat aataaggaag cgtatgatgt tgtgatactt tctaagatac gttgatgtaa
     2521 ttctgttgtt tcgatggctt ggttattttc aatgattaaa tcttctagta attcttcatc
     2581 ttcttcaaaa cgtttaattg caggtaaacg gaataatttt ttaataattg tatcgttacc
     2641 ttttaaggca gctaaaaagt atactaaact tttttcaact tccattaatt tgaaaagttg
     2701 tttgttagta atgttatttt ttagttcttt ctcaatacga attctacttt tattaagtaa
     2761 tcgtaagtta cgattataat ggtcggcaat agtagttaaa atttctaatg caaatctgct
     2821 gtgaaatttt aaattaatat cacgcttagg taaattttct aaaaattcat tttcagcgtc
     2881 acaaactgtt acgataatac cgttgccaat aataatgcct aatggaatcg ttacaaaaga
     2941 tagaacgcta cgattagttg aattgacgat aggtaaatca ataatgatta atgagtaacc
     3001 agtatcttcg tcatattcaa tacgggaact ttcttctgag tccaaagggt cacgtataaa
     3061 gtcttcaggt atattatatt gttccataag attttcaatt tcttctcggt ctggttctac
     3121 tacattaatc catgacgctg tatgatctaa tggtgttttt ataatatgtt tatctatatc
     3181 ttcagaatgt ctgaatgaag taatcatgat atatagttcc tttctaaagg atttaggaaa
     3241 agcgagtatt acttaagtta gtgaatcaag gtttgattta tgcaaacggt tttctgccat
     3301 tattcttaat ggaccattaa aaacaatatt atgcacatgt ttagccaatt catcaactga
     3361 aatgggctgt ttgtccatta cccagtattt aataattgaa attgttgctc cggaaacgta
     3421 actgtaaaaa tacatctctg gaatgcctcc gatagaatga ttgatactaa tataacgttg
     3481 catattattt ttaatatgtt cgttgatttt caattctaat tgactcgtgc gttccaattg
     3541 taaaatagta tgataaaact ctaaattatc atgaatatgc tggaaaatat ttttgagtat
     3601 attatttaca aaagtattcg caatgtcttc tattgaagaa ccgcgtaaca aatcaaattg
     3661 agtatatgtc gttagttctg aaatatactc atcttccata tctgcgagta acatatattt
     3721 atcctcgtaa tgtaagtaaa atgtccctct attgatatca gcgcgtgttg ttatgtcgcg
     3781 aatggtaatc ttttctaaat ctttttcttg tagtaattta gtgaacgctt gtttaatgga
     3841 ggactttgtc tttctaattc gcctatcttc tttcaaaaat aaatcaacct ccttgtgtta
     3901 ttgaacaatg tgggtgtatt tgtctattaa taaacgtatt gatgaacatt gtgtattgtt
     3961 tattcccact tacatattat aatagacaac atgcgaaata ttcaacacat tgtctaataa
     4021 ggaggtaatt aataaaatga atatatttaa aaataaatta ttatggattg caccaatagc
     4081 aactatgatt atcttggtaa ttttttcttt agctttttat cctgcatata atcctaaacc
     4141 aaaagattta ccaattggta tattaaacga ggacaaaggt acaacgattc aagataaaaa
     4201 tgttaacatt ggtaaaaaat tagaggataa attattagat agtgattcta ataaaattaa
     4261 atgggttaag gttgatagtg aaaaagacct tgaaaaagat ttgaaagatc aaaaaatctt
     4321 tggagtagct attattgata aagacttttc aaaagatgct atgagtaaaa cacaaaaagt
     4381 agttatggat agcaaaaaag aagaaatgca acaaaaagtt gcttcaggtg aaattccgcc
     4441 acaagtagtt caacaaatga aacaaaaaat ggggaatcaa caagtagagg ttaagcaggc
     4501 taaatttaaa acgattgtaa gtgaaggatc aagcttacaa ggttcacaaa ttgcatcagc
     4561 tgtattaact ggtatgggtg ataatattaa tgctcaaatt acgaagcaaa gtttggaaac
     4621 attaacgagt caaaatgtta aagtcaatgc cgcggacatc aatggtttga cgaatccagt
     4681 aaaagtggat aatgaaaaac ttaataaagt taaagatcac caagcaggtg gtaatgcacc
     4741 attcctaatg tttatgccaa tttggatagg ttcaatcgta acgtctatct tattgttctt
     4801 tgcatttaga actagtaaca atatcgtcgt gcaacatcgt atcattgctt caattggaca
     4861 aatgatattt gcagttgttg cagcatttgc aggtagcttt gtttatattt atttcatgca
     4921 aggcattcaa ggatttgatt ttgaccatcc aaatcgtatc gcaatttttg tagcacttgc
     4981 gattcttggt ttcgtgggcc ttattttagg tgttatggta tggctaggta tgaagtcaat
     5041 tccaattttc ttcattttaa tgttctttag tatgcaactt gtaacgttac ctaaacaaat
     5101 gttgcctgaa agttatcaaa aatatgtata tgattggaat ccattcacac actatgcaac
     5161 aagtgtaaga gaactattat acttgaatca tcatattgaa ttaaatagta caatgtggat
     5221 gtttataggg tttatgattt ttggcgctgt atcaagttta atttcagcta ttgttagaaa
     5281 acatagcaca aaacgtactg aagtgccgtc ataatttaat aaataggaat atagctatac
     5341 aagacgtttg tagcgagcgt catggtatag ctattttatt tgtatagcac ttgatgtaaa
     5401 gttattttag ctgtcatact atgtgctaaa tcaaattcag ttgtgtcata tcttcgcttt
     5461 tttgtcactg ttataatagg tataatcgga tataatgaaa aggtgaggga ggattcaaaa
     5521 tgcgtaggtt attatattca tttctttttt atatgatcat aggtttattt agtggctttt
     5581 tctatagaga gctaacaaaa gcatatgatt ttactggtac aacacaatta tcacttgtac
     5641 acacacatac acttatttta ggtatgttta tgtttttaat attattacca ttggagaagt
     5701 tatttaaatt atcaagttac tacttattta actggttctt ctatgtatat aacataggtg
     5761 ttatcgttac tataggtatg atggtgacaa aaggattctt ccaagtcact ggtaaatcat
     5821 actctccaga agcatttgca ggctttgcag gtattgggca cacaggtatg cttgcaggtt
     5881 tattgttgct atttttcctt ttaagacaag caattcttaa agaaccacgt gatgagtctt
     5941 ttaaaaaata atatgaacta aaaaatatac acatctttca aaggaacatt gttgtttcgt
     6001 actttgaaag gtgtgttttt atattgtgat gggatagtta ttgataattc atggctaact
     6061 atgcgattta ttttaacaat ataaatatta atagtataat gtctttaaat gataataatt
     6121 atcaatttga gcgcgttaat gaaagaaatg aatatagaga gtcataggag gcacataatg
     6181 aaagacgtta caatcattgg tggcggtccg tctggtttgt atgcaagctt ttatgctgga
     6241 ttgcgagata tgtctgtaag attgatagat gttcaatctg aattaggggg taagatgaga
     6301 atttaccctg aaaaaattat ttgggatatt ggcggcattg cgccaaaacc ttgtcatgaa
     6361 attttaaaag atacaattaa gcaaggatta tattttaaac cggaagttca tttgaatgaa
     6421 cgtgtggtag atattaggaa aaaatcagaa cgtcatttcg aagttgaaac agaagcggga
     6481 gaaatatata catcgaaagc agttatcatt gctattggtg caggtattat taatccaaaa
     6541 caattagatg ttaaaggtgt agagagatat caattaacta atttacatta tgttgtacaa
     6601 agttacagac gtttcaaaga taaagatgta ttaatatcag gtggaggaaa tacagcatta
     6661 gattgggcgc atgatattgc taagattgct aaaagcgtga cagttgttta tcgaaaagaa
     6721 gatgtaagtg gtcatgaagc gatgaaaacg ctggtgacag atttaaatgt gaaactatgc
     6781 ccgaaaacac gtattaaata cttggtcggc aatgatgatg aaacgcatat aagtgaagtt
     6841 gttttggaac atgttgaaag tggtgatacg catgcagtta aatttgacga tgtcattatt
     6901 agtcatggtt ttgatcgttg taatacatta ttgagtgaaa catcttcaaa gctagatatg
     6961 catgatgatt gtcgtgttaa aggatttggt aatacgacaa ctagtatacc tggtatctat
     7021 gcgtgtggag atattgttta tcatgatgca aaatcacatc taattgcaag tgcatttagt
     7081 gatggtgcaa atgcagcgaa tcttgctaaa acatatatcc aaccagatgc gaatgcagag
     7141 ggttatgttt caagtcatca tgaagtgttt aaagaagcga ataagactat tgtaaataaa
     7201 catttatact aggttgatat attgtgtgat gaaaagtggg gtaaataatg gttaaagtga
     7261 catatgatat tccgacttgc gaggattatt gcgcattaag gattaatgca ggtatgagtc
     7321 caaagacgcg cgaagcagct gaaaaaggat tacctaatgc cttatttaca gtaaccttgt
     7381 atgataaaga taggttaatt ggtatgggta gagtgattgg cgatggagga actgtttttc
     7441 aaattgttga tattgcagtt ttgaaaagtt accaaggtca aggttacggc agtcaaatta
     7501 tggaacatat tatgaagtat attaaaaatg tggctgtcga aagtacctac gtaagtctga
     7561 tcgcagacta cccggcggat aaattgtatg caaaatttgg atttatgcct accgaaccag
     7621 attcaggcgg tatgtatatc aaatactaaa aatgaaaaat cgtaccgata aacatactga
     7681 gttttctaca cagcttaact tagtatgttt ttgtttttct aagtaatgat attaaaaaaa
     7741 tgtgtgtttt atggtaaagt tcaagttgat atgatataga agtttcgact ttgtaactaa
     7801 gggagttaga acaatgaaaa tcgatacatt aagacaagct ataatatatt attttgttgc
     7861 acttgtgtta tcagctttaa tgatctattt atttttaaca aacattgaga gagttatttt
     7921 gtatagttgt gtattcacag tcgtttattg ggcaatatat gcctttgatg tatatagaaa
     7981 gagaaattaa attttatttt tagacacagg ctgggacata aatcaatatt ctatgctcta
     8041 cgaagttata ttggcagtat ttgattagtg ttgcaagtat gataggggcg gagaatacat
     8101 gattccctaa aatgtattaa agtaaagtgc tatttgatga agtatcagaa attttataaa
     8161 ccaaaagaaa ttaaaaaaga catctcgcat aacgggatgt ctttttcatg aatactattt
     8221 atctacaccg aaatcgataa caatgcggcc tttattttca tgtttaatta ctttgttaag
     8281 ttgttctgga agttcatcaa atgtaataac ttgcttgatc tcatgtaaat tttcaggctt
     8341 taaatctttt gcgagacgac gccaaacgcg ctgtcttaat ttcatagcag taaatactga
     8401 gtcaattcct aaaatgttta cgcctcttaa aatgtgaggg aatacagaat tagtataagt
     8461 attaccggca gtcataccaa taactgcaat tgatccacta tgatttaaac gctttgtaac
     8521 ataattaata ccttcaccac caacagggtc tacacaagct tgccaagttg atgatgcgag
     8581 tggcttttta tgatcatctt caacaggaag tcgatcgata acttctttgg caccaagttc
     8641 aagtaattga ttgctaacat cttgtttacc tgtacttgcg ataactttat aaccaagttc
     8701 attaagcatg agtactgcta aagtaccgac accacctgaa gcaccgcgaa cgagtacagg
     8761 accatcttca atattcattc caactttttc aagtctttca attgctaaac cggcagtata
     8821 accagctgtg ccatatatca ttgattcttc taatgttaaa gtatcaggaa gcttgacaat
     8881 ccattctgat tttacacgcg catattcact aaaaccgcca taatggctga cacctaggtc
     8941 ataactcgtt acaattactt gttctccttt ttcaaagcct ggtgcttcgg attcaacaat
     9001 tgtaccagct aaatctattc ctggaatcat aggatacgat tttacgactg cattatgatt
     9061 ttgagtcgct aaagcatctt tataatttat accagagtaa tgtactttaa tcagcacatc
     9121 tcctttaggt aaatcagtag tcgataattg tttgaaagtt ggtgtcactt taccgctctc
     9181 atctttatca attacaaacg ctttaaatgt ttctgtcaca acgatacatc ctctcttttt
     9241 ctcttttaaa aatacggcca ttttactgga ataaatgtat catttattga tgttacacac
     9301 aatcaataca atttgtaatt gatttgtgtt aatgtatttt taaatagttt tggcagaaaa
     9361 atacataagt aatattttga ttcgattact gtttattgga ggatttatat ggctgggata
     9421 atcaaagaaa tttcaaaaca agatgctagt gaattagttg aattagcaac tagaacattt
     9481 tatgacacgt ttggttctta ctatgatgac aatgattttg atcaattttt taaagataat
     9541 tatactgtgg aaaaatttac acaagagatt aaccatgtag attcatttca ttatttttat
     9601 caagaagatg gtgcgaatgt tggttatata aagatgaata ttaatagtgc tcaaactgaa
     9661 gaaatggggg agacctattt agaagtacag cgcatatact ttttgaaaga ctttcaaggt
     9721 ggtggaagag gttcacaatt gatagcattg gccgaaaaaa ttgcacaaga acataataaa
     9781 cataaaattt ggctaggagt ttgggagcat aatcctcgtg ctcaagcatt ttataaacgt
     9841 catggattta aagtggttgg tgaacatcat tttcaaacag gtgatgtaac ggatacagat
     9901 ttaattatgg aaaaagagtt atagcagtaa cttctgttgt cgataaaaag aaattgctgt
     9961 tgtaatcatt gaatatgtat agaaatgtaa aatgtgtaat aaaattgttg agtaaaatga
    10021 ttaaatgtat aaaaaacata aaattcgcaa aaattagata ataatgatta aaatttaggg
    10081 tataataaaa agaaccatag atgatagttg ttgatattgg gggaaataaa acaccatatt
    10141 attgttgacg tctttggtaa caatccatga aatgatacac atggtagata tgtatttcaa
    10201 gcttattcaa tgaatatcga attataggag gagatatgta tgaaaaaatt agttacaggg
    10261 ttattagcat tatcattatt tttagctgca tgtggtcaag atagtgacca acaaaaagac
    10321 agtaataaag aaaaagatga taaagcgaaa actgaacaac aagataaaaa aacaaatgat
    10381 tcatctaaag ataagaaaga caataaagat gatagtaaag acgtaaacaa agataataaa
    10441 gataatagtg caaacgataa ccagcaacaa tctaattcaa atgcaacaaa caatgaccaa
    10501 aatcaaacga ataataacca gtcaaacagt ggacaaacga ctaacaatca aaaatcaagt
    10561 tacgttgcac catattatgg acaaaacgca gcgccagtgg ctcgtcaaat ttatccattt
    10621 aatggtaata aatcacaagc attacaacaa ttgcctaatt tccaaacagc tttaaatgca
    10681 gctaacaacg aagcaaataa atttggtaat ggtcataaag tttataatga ttattcaatt
    10741 gaagaacata atggtaacta taagtatgtt tttagtttta aagacccaaa cgtaaatgga
    10801 aaatattcaa ttgtaacggt tgattatact ggacaagcaa tggttactga tccaaactac
    10861 caacaataat gttaatgtaa ttatgatgca agttaaaaaa ttaaaacggt aaactctata
    10921 atatgaatta gggtttaccg ttttttgcgt attttaaagt aaaaaggttt acgttctata
    10981 gtacttttaa acctcattca cagccactga ggaatttaag cactatttat tagatgtcat
    11041 tttagacaat ataagttgtg aaatagattc taatggttgt tctttctgtt gtatttgtgg
    11101 atatttaatc actggtattg cctgggaaat taaattatta attaataatt gtcggtctgt
    11161 ataccaaatt tttgatagat gacgccaaat atcttcgtta acaccttgag actcaatata
    11221 ttgactgtga tttggagtgt ataatgctac tgttttgtct atagccatcg catcaaatat
    11281 tatatttgag taatcagtga taacgacatc agacattaga attaagtctt gagcactgag
    11341 atactttggt gcaacaagcg cctcttctgg taacatcgtt tcatcaacac cctgaaccac
    11401 aacatgatag gctttgaata aagcatctga taatggtaat tgttgcgcac tcactaatcc
    11461 aataggtgca tataataaaa ctggtttttg atcatttatt ttaaacgatt tcttatactg
    11521 ttgttgagta gtactttcgt tacgcttttg tagtaaatat tggtgtttag gattaccata
    11581 agccaataca tttgcttggt gactaggaaa agccgtttga taaaagtggc taatatcatc
    11641 agcagaatgt ataacataat cgaattgcaa ccagcgatta taccttttag cacgataaaa
    11701 aggtgtttct atatttttaa taggttcttt actatctaaa aataattgca ttattggtgt
    11761 gccttgatgt aattgaatta aagtaccgtt tggttgcaac gtttcaggta tatcattttc
    11821 aactaaaacg acacgtgcgc tgttaatcaa ttcgtcagct ttttcagact taggtgatat
    11881 gaaatgtggt ccgcgacgat cgtcagtaac aaaataacag gcagtcattg gattatgttt
    11941 aacgaagtaa gtaaataaat attttgaatt acctatatat tgataatgat caccgataaa
    12001 aacgatgtga tcttttaaaa cgtttggttg cttcgtatac aggccgaggt tttgtaattg
    12061 tttgttatca gcacgttgct ttgctatttt atataaccat ttaggtagca taaacttggt
    12121 agaatacaac caactatctg gccaattaag ttgttctgaa tcctcgataa aaaggttgcg
    12181 aaaaggtata ggatgatgat cgatataagc cattttattc aaataaaact gagacatcat
    12241 atcaaaatct tcaatagttt taatagtttc ccatattgaa gcacttgttg ctaaaggggc
    12301 tttaaaatca aaaacgacat gaccatcgat tgataaatca cctttcagaa gtgtcaataa
    12361 agctaaatcg aaaatcgttt ctacagagta ataatgtatt aaatcaagag tctgttgaat
    12421 cgcatatatc aggttaggat aataattatt gttttgagcc caatcggcaa tgttgatcga
    12481 tgattgaccg aaatagtgac aatcattttt gtattgtcca tccacagtat aatcaaccac
    12541 gatagactta ggatataatt gaaccgcatc taacatagat tgataaatca ttatctcatt
    12601 ggcatatgga acggcatgtg tataaccttc agactgagct ttcattaatt gagattcaaa
    12661 cttttgcata ttagaaacat ttatctgttt aatcattgct ataacttcca tttctcatcg
    12721 tagtatcttc ct
//
LOCUS       DAKSQE010000077        12638 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.077, whole genome shotgun sequence.
ACCESSION   DAKSQE010000077 DAKSQE010000000
VERSION     DAKSQE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12638
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.077"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            26..259
                     /locus_tag="O0V33_001870"
     CDS             26..259
                     /locus_tag="O0V33_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCX8976494.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            370..1635
                     /gene="murA"
                     /locus_tag="O0V33_001871"
     CDS             370..1635
                     /gene="murA"
                     /locus_tag="O0V33_001871"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCX8976495.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRGAIKEHMASLVYKLEEMGVE
                     LEYQEDGIRVRAEGDLQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            1669..2109
                     /gene="fabZ"
                     /locus_tag="O0V33_001872"
     CDS             1669..2109
                     /gene="fabZ"
                     /locus_tag="O0V33_001872"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCX8976496.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(2166..2606)
                     /locus_tag="O0V33_001873"
     CDS             complement(2166..2606)
                     /locus_tag="O0V33_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HCX8976497.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            2800..3195
                     /locus_tag="O0V33_001874"
     CDS             2800..3195
                     /locus_tag="O0V33_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCX8976498.1"
                     /translation="MLNKIVIVGRLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIERYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSILDIDSQNIDNHDLLEI"
     gene            3584..4279
                     /gene="sceD"
                     /locus_tag="O0V33_001875"
     CDS             3584..4279
                     /gene="sceD"
                     /locus_tag="O0V33_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HCX8976499.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAQASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGANGADMSAQAPATNNV
                     APSADQSNQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKASEGSSVNVNDHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     regulatory      4622..4724
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4804..5493
                     /gene="tenA"
                     /locus_tag="O0V33_001876"
     CDS             4804..5493
                     /gene="tenA"
                     /locus_tag="O0V33_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HCX8976500.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLLGNIQADALRHYLQAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFESLMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     gene            5486..6316
                     /gene="thiD"
                     /locus_tag="O0V33_001877"
     CDS             5486..6316
                     /gene="thiD"
                     /locus_tag="O0V33_001877"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCX8976501.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNNTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGVIIKGGHSNDADIAKDYLFTKEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRPLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            6300..7091
                     /gene="thiM"
                     /locus_tag="O0V33_001878"
     CDS             6300..7091
                     /gene="thiM"
                     /locus_tag="O0V33_001878"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCX8976502.1"
                     /translation="MNYLNNIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQALLINIGTLTAQNEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVTIAKKAYAIYKTAIVIT
                     GKEDVIVQGDKAIVLANGSPLLARVTGAGCLLGGIIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE"
     gene            7093..7734
                     /locus_tag="O0V33_001879"
     CDS             7093..7734
                     /locus_tag="O0V33_001879"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCX8976503.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGFSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHTPVGPEMIATFKEM
                     NPQLPIVAIGGINTSNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN"
     gene            7820..8692
                     /gene="yidC"
                     /locus_tag="O0V33_001880"
     CDS             7820..8692
                     /gene="yidC"
                     /locus_tag="O0V33_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCX8976504.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(9305..9952)
                     /locus_tag="O0V33_001881"
     CDS             complement(9305..9952)
                     /locus_tag="O0V33_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCX8976505.1"
                     /translation="MQQSDIISAAKKYMESIHQNDFTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKQYVELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEQMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQFLTEWNYHD"
     gene            complement(9980..11464)
                     /gene="cls"
                     /locus_tag="O0V33_001882"
     CDS             complement(9980..11464)
                     /gene="cls"
                     /locus_tag="O0V33_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCX8976506.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKVYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLHPFRNKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL"
     gene            11630..11923
                     /gene="csoR"
                     /locus_tag="O0V33_001883"
     CDS             11630..11923
                     /gene="csoR"
                     /locus_tag="O0V33_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HCX8976507.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            11935..12144
                     /locus_tag="O0V33_001884"
     CDS             11935..12144
                     /locus_tag="O0V33_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HCX8976508.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVIINIIDGQVT
                     VAYETPANLNSIEKEIYDEGYKIVF"
     gene            12272..12409
                     /locus_tag="O0V33_001885"
     CDS             12272..12409
                     /locus_tag="O0V33_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976509.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
BASE COUNT         4460 a         1971 c         2284 g         3923 t
ORIGIN      
        1 tacagtaatc ttatgaggtg ataaaatgga ttatatcgga caatatgcag ttatccattt
       61 agtgttacat gttgtatgta tttgtattgc ctattgggct ttacaatcaa ttagattaga
      121 tcaatttttt aaaaaaggat atgccactca attacaagtg tgtatgatat ttgttgctat
      181 tttattaggc actgcagtaa gcaattttat tgtagatttg ttacaatact cgacgcaggt
      241 aaaatattta ataaaataag tctaactcta tgatttgtaa tcaaaactag atataattaa
      301 ataatgactt aaaataattt taaaataggg aaatgtaaag taataggagt tctaagtgga
      361 ggatttacga tggataaaat agtaatcaaa ggtggaaata aattaacggg tgaagttaaa
      421 gtagaaggtg ctaaaaatgc agtattacct atattaacag catctttatt agcttctgat
      481 aaaccgagca aattagttaa tgttccagct ttaagtgatg tagaaacaat aaataatgta
      541 ttaacaacct taaatgctga cgttacatac aaaaaggacg aaaatgctgt tgtcgttgat
      601 gcaacaaaga ctctaaatga agaggcacca tatgaatatg ttagtaaaat gcgtgcaagt
      661 attttagtta tgggacctct tttagcaaga ctaggacatg ctattgttgc attgcctggt
      721 ggttgtgcaa ttggaagtag accgattgag caacacatta aaggttttga agctttaggc
      781 gcagaaattc atcttgaaaa tggtaatatt tatgctaatg ctaaagatgg attaaaaggt
      841 acatcaattc atttagattt tccaagtgta ggagcaacac aaaatattat tatggcagca
      901 tcattagcta agggtaagac tttaattgaa aatgcagcta aagaacctga aattgttgat
      961 ttagcaaact acattaatga aatgggcggt agaattactg gtgctggtac agacacaatt
     1021 acaatcaatg gtgtagaatc attacatggt gtagaacatg ctatcattcc agatagaatt
     1081 gaagcaggca cattactgat cgctggtgcc attactcgtg gcgatatttt tgtacgtggt
     1141 gcaatcaaag aacatatggc tagtttagtg tataaattag aagaaatggg cgttgaattg
     1201 gaatatcaag aagatggtat tcgtgtacgt gctgaaggag atttacagcc tgttgacatc
     1261 aaaacattac cgcatcctgg ttttccaacc gatatgcagt cacaaatgat ggcattatta
     1321 ttaacagcaa acggacataa agtagtaact gaaactgtat ttgaaaatcg ttttatgcat
     1381 gttgcagagt tcaaacgtat gaatgctaat atcaatgtag aaggtcgtag tgctaaactt
     1441 gaaggtaaaa gtcaattgca aggtgcacaa gttaaagcga ctgatttaag agcagcagca
     1501 gccttaattt tagctggatt agttgctgat ggtaaaacaa gcgttactga attaacgcac
     1561 ctagatagag gctatgttga cttacacggt aaattgaagc aattaggtgc agacattgaa
     1621 cgtattaacg attaattcag taaattaata taatggagga tttcaaccat ggaaacaatt
     1681 tttgattata accaaattaa acaaattata cctcacagac agccattttt attaattgat
     1741 aaagttgttg aatatgaaga aggtcaacgt tgcgtggcta ttaaacaagt atcaggaaac
     1801 gaaccattct ttcaagggca ttttcctgag tatgcggtaa tgccaggcgt attaataact
     1861 gaagcgttag ctcaaacagg tgcggtagct attttaaata gtgaagaaaa taaaggtaaa
     1921 atcgctttat ttgctggtat tgataaatgt cgttttaaac gtcaagtagt acctggtgat
     1981 actttaacgt tggaagtaga aatcactaaa attaaaggac caatcggtaa aggtaatgct
     2041 aaagctactg tcgatggtca acttgcttgt agttgtgaac ttacatttgc aattcaagat
     2101 gtaaaataaa acaaaaaaca ttcaaagatt taatgtgttg gcataatctt tgaatgtttt
     2161 ttattttact cttctaattt ttcatccttt aactttggtt tagactgcat cattcgatta
     2221 aatgattttt ttaattcttc accagataat ccatcatcaa taagttgttc taataaactt
     2281 tcagcatact gttgacgtaa agtgtaaata tgacattcaa atacgataga cattgttgtt
     2341 ttaaaatttt taatactttt cacagtagat tcaaataaag ctggatcatt ggctggacgc
     2401 gtgatgacga cacgaatatc caataactgt tcattgtctt gatattcctt aaatgtttca
     2461 taatgctcat tagaaatgac aatttcaagc agccaacccg tgccactttc ctctttatta
     2521 attattacgc catcttctaa ttcatattct ataattctgc cgtgttcatt cacaatttga
     2581 aaccttactg ctttaaaagt tttcatattc ccacatccca ttagtttaat atttatgatt
     2641 tttgaattat ttagaagatt taaggtcatt tcatgactaa tttctttaaa tataacaaat
     2701 aacgagatgc tatagctatt aattatataa aatttgccat tttgaccatt tttaagctag
     2761 taattaagat ataggcatag gaggtgaatc acacactaca tgctaaataa aatcgtaatt
     2821 gtcgggagac tgacgaaaga cgcacaaata tttgaaaagg aggatagaaa aattgcaacg
     2881 ttttgtgttg caacgcaccg aaattataaa gatgaaaatg gagaaatcgt ctgtgattac
     2941 ttattctgta aagcatttgg caagttagct tctaatatag aaagatacac taatcaaggt
     3001 acattggttg gtataactgg tcaaatgaga tcaagaaagt atgataaaga cggacaaaca
     3061 cactttgtca ctgaattata tgttgaaaca ataaaattta tgtcccctaa atcccaaaat
     3121 aatgaaattc tctcagatag tattttagat attgactctc aaaatataga taatcatgac
     3181 ttattagaaa tttaaatcca attatcctaa acatccttaa tatacattta atccatccta
     3241 aattttttat agatctaatt aacttgaaat atactcactt aaaaacgact tacatgacta
     3301 gtactgttgt gtaggtcgtc tttttgtgta gtttttctaa tagaaaatag acatagattg
     3361 caaaacgatt acataaaaaa ttgcaaatta aaaacatttt tactgtcatg acaattctcg
     3421 atagaatcat atctgaaatt atggaggatt ccgttgtaat cactgtgtaa aaaacaaggc
     3481 tattttttta ttttgttgta acctgcacgg attttacaga tgttacagta gtctctgtaa
     3541 aacttttaga gaaacaaatt acttgtagga ggattttaag attatgaaga aaacattact
     3601 cgcatcatca ttagcagtag gtttaggaat cgtagcagga aatgcaggtc acgaagccca
     3661 agcaagtgaa gcggacttaa ataaagcatc tttagcgcaa atggcgcaat caaatgatca
     3721 aacattaaat caaaaaccaa ttgaagctgg cgcttataat tatacatttg actatgaagg
     3781 gtttacttat cactttgaat cagatggtac acactttgct tggaattacc atgcaacagg
     3841 tgctaatgga gcagacatga gtgcacaagc acctgcaact aataatgttg caccatcagc
     3901 tgatcaatct aatcaagtac aatcacaaga agttgaagca ccacaaaatg ctcaaactca
     3961 acaaccacaa gcatcaacat caaacaattc acaagttact gcaacaccaa ctgaatcaaa
     4021 agcatcagaa ggttcatcag taaatgtgaa tgatcatcta aaacaaattg ctcaacgtga
     4081 atcaggtggc aatattcatg ctgtaaatcc aacatcaggt gcagctggta agtatcaatt
     4141 cttacaatca acttgggatt cagtagcacc tgctaaatat aaaggtgtat caccagcaaa
     4201 tgctcctgaa agtgttcaag atgccgcagc agtaaaacta tataacactg gtggcgctgg
     4261 acattgggtt actgcataag ccatttatgc atagctaacc aatagttata taagtaactt
     4321 ttagattgga atatatcggg gaattctcga tatgttccgg tcttttttat ttcgcaaata
     4381 tatataattg tacaggctgg gacataaatc aatgttctat gctctacgaa gttatattgg
     4441 cagtagttga ctgaacgaaa atgcgcttgt aacaagcttt tttcaattct agtcaacctt
     4501 gccggggtgg gacgacgaaa taaattttgc gaaaaaatca tttctctccc actcccaact
     4561 gagcaacaat aattgaaaat gttgaaaccg tttgctaaga ttaatttgca attgaatact
     4621 aaggaactac taggggagcc taatgatatg gctgagatga actgttcaga cccttatgac
     4681 ctgatttggt tagtaccaac gtaggaaagt agttattgaa gtgatggggc tagtatagca
     4741 acattgccat caaactactt tcatgttgga agtagttttt ttatttaggg aggatttttt
     4801 acaatggaat tttcacaaaa attgtaccaa gctgcaaagc caatcattaa tgatatttat
     4861 gaggatgact ttattcaaaa aatgttatta ggcaatatac aagctgatgc attacgtcat
     4921 tatttacaag ctgatgcagc ttatttaaaa gaatttacaa acttatatgc tttgttaatt
     4981 ccaaaaatga atagcatgaa tgacgtaaaa tttttagtag agcaaataga atttatggtt
     5041 gaaggtgaag tacttgcgca tgatatttta gcgcaaattg ttggtgaatc atacgaagaa
     5101 atcattaaaa caaaagtttg gcctccaagt ggcgaccatt acataaaaca tatgtatttc
     5161 caagcgcata gtcgtgaaaa tgctatttat acaattgctg caatggcacc ttgtccatat
     5221 atttatgcag aattagctaa gcgctctcaa agtgatcata agcttaatag agaaaaagat
     5281 acggcaaaat ggtttgattt ttatagtact gaaatggatg acataattaa cgtatttgag
     5341 tcattaatga ataaattagc cgaatcaatg tctgacaagg aattggaaca agtaaagcaa
     5401 gtatttttag aaagttgcat acatgagcgt cgatttttca atatggctat gacactagaa
     5461 caatgggaat ttggaggaaa agtaaatgat taaacctaaa atagcattaa ccattgcagg
     5521 tactgatcca acaggtggtg ccggcgtaat ggctgattta aaatcatttc attcatgtgg
     5581 tgtatatggt atgggcgtcg ttacaagtat tgttgctcaa aatacattgg gcgtacaaca
     5641 tattcataat ttaaatcatc aatgggtaga tgaacaactt gatagtgtct tcaatgatac
     5701 cttaccgcat gctattaaaa cggggatgat tgctacagca gatactatgg aaacgattcg
     5761 tcattattta atgcaacatg aatctattcc gtatgtaatt gatcctgtta tgttggcgaa
     5821 aagcggtgat tcattaatgg ataataacac aaagcaaaac ttgcaacata cgttattgcc
     5881 attagctgat gtagtaacac cgaatttacc agaagctgaa gaaataacgg gactaaccat
     5941 tgatagtgaa gaaaaaatta tgcaggctgg ccgcatcttt attaatgaga ttggtagtaa
     6001 aggtgtcatc attaaaggcg gtcattcaaa tgatgctgat atagcaaaag attatttatt
     6061 tactaaagaa ggtgttcaaa catttgaaaa tgaacgattt aaaacaaaac atacgcatgg
     6121 aacagggtgt acattttcag cagttataac ggcagaactt gcaaaaggta gaccattatt
     6181 tgaggctgta cacaaggcta aaaagtttat ttcaatgagt atacaatata cgcctgaaat
     6241 cggccgtggt agaggtccag tgaatcattt tgcatattta aagaaagagg gattagacga
     6301 tgaattatct aaataacata cgtattgaaa acccattaac aatttgttat acaaacgatg
     6361 tagttaaaaa ttttacagcg aatggtttat taagtattgg tgcgagccct gcaatgagtg
     6421 aagctcccga agaagctgaa gaattttaca aagttgcaca agcgctatta atcaatatcg
     6481 gtactttaac agcccaaaat gaacaagata ttattgcgat tgctcaaacg gcaaatgagg
     6541 caggcttacc tattgtattt gaccctgtag ctgttggtgc ttctacatat cgaaagcaat
     6601 tttgtaaatt attattgaaa tcagcgaaag tatcagtaat taaaggcaat gcatctgaaa
     6661 tattagcgtt gattgatgat acagcaacta tgaaaggtac agatagtgat gctaatcttg
     6721 atgcggttac aatagcgaaa aaggcttacg caatatataa aactgcaata gtaatcacag
     6781 gtaaagagga cgttattgtt caaggtgata aagcaatcgt attagctaat ggatctccat
     6841 tattagcacg agtaactgga gctggttgtt tattaggagg cattattgct ggatttttat
     6901 ttagagaaac agaaccagac atagaagcgt taattgaagc ggtaagcgta tttaatattg
     6961 ctgctgaggt agctgctgaa aatgaaaatt gtggtggtcc tggtacgttt tcaccattgt
     7021 tgcttgatac gttatatcat ttaaatgaaa caacctatca acaacggatt cgcattcaag
     7081 aggtggaata atatgtttaa ccaatcgtat ctaaatgtgt attttatttg tggaacctcc
     7141 gatgttccga gtcatcgaac tattcatgaa gtattagaag cagcattaaa ggcagggatt
     7201 acacttttcc aatttcgaga aaagggtgaa tcagctttaa aaggaaacga taaacttgta
     7261 ttagctaaag aattacagca tctctgtcat caatatgatg tgccttttat cgttaatgat
     7321 gatgtatcct tggcaaaaga aattaacgcg gatggtatac atgtcggtca agatgatgcg
     7381 aaagtcaaag agatagcaca atatttcact gataaaatta ttggatttag tattagtgat
     7441 ttagatgagt atgcaaaatc tgatttaact catgttgatt atataggtgt tggaccaatc
     7501 tatcctacgc cgtcgaagca tgacgcgcat acaccagtag gtccagaaat gattgcaaca
     7561 tttaaagaaa tgaatccgca actaccgatt gtagccattg gcggtataaa tactagtaac
     7621 gtggcgccga ttgtagaagc aggtgccaat ggaatttcag tcatttcagc catttctaaa
     7681 agtgaaaata ttgaaaagac tgttaatcga ttcaaagatt tttttaataa ttagttaaat
     7741 ttttccactt attgtttcat acgtactcat taatgtgata gaataaatcc tatgtgaata
     7801 taatgataga ggtggaaaaa tgaagaaaaa agcgttacta ccattatttt taggtattat
     7861 ggtctttttg gctggttgtg actattctaa acctgaaaaa cgtagtgggt ttttctacaa
     7921 tacattcgta gatccaatga aaaatgtatt ggattggttg ggaaataact tattaaacga
     7981 caattatggt ttagctatta ttattcttgt attggtaatt cgtattattt tattaccatt
     8041 catgttgtca aactataaaa atagtcatat gatgcgtcaa aaaatgaaag ttgcaaagcc
     8101 agaagttgag aaaattcaag aaaaagtgaa gcgtgcgcgt acacaagaag aaaaaatggc
     8161 tgcaaaccaa gaattaatgc aagtatataa aaagtatgac atgaacccga ttaagagtat
     8221 gttgggttgt ttaccaatgc taatccaatt gccaatcatc atgggattat actttgtact
     8281 taaagaccaa cttgtagatg gtttgtttaa atatccacac ttcttatggt tcgatttagg
     8341 acgtcctgat atttggatta caattattgc cggtgtttta tactttatcc aagcatatgt
     8401 atcaagtaaa acgatgccag acgaacaacg tcaaatgggt tacatgatga tggtcatttc
     8461 accaattatg attatctgga tttcattaag ctcagcatca gcacttggtt tgtactggtc
     8521 agtcagcgcg gcgttccttg tagttcaaac acactttgcg aacatttatt atgaaaaagt
     8581 cgctaaaaaa gaagtacagc ctttcattga agcgtatgaa agagagcaca acggcggcag
     8641 caataaaaaa ggcaaaaaca cacaagtagt gtctaaaaag aaaaagaaat aaccttagca
     8701 agtccaaata atgtgccccc taaaagttga agataatttt aactttatag ggagcacatt
     8761 atttttcgct tcaatagcga aacttagtgt cacattatac aatatgtgat aaatagaaac
     8821 agcactaaga tgatttcaaa tgtgaaagtc atcctactgc tgtttttatt tgaaataata
     8881 ttaacgagat ttcaccatgt aaaaagtggt taataacaga gttgctattc ttcattgcaa
     8941 tgtcatcttg acacaatgat tcatcagtgt agcagtatat gtcgcaacat ttccacagca
     9001 attcacaaat acatgtaaca gtgtgaaata ataacgtcac aacggcataa tacacattgc
     9061 aacgtcccaa cgacatatca tagaattgta acggcatggc atcaatacca cgggctatgc
     9121 cacccataac accgcaacgc cacatcatag cactacatca agacgcatcg tggtactagc
     9181 acatcaaagt agcatggcat caacaccaag ctcccacaac aacacatcaa agtaacatgg
     9241 catgacacca agcttccaca acaacacgct attacatcgc atcatcatac tacaactcca
     9301 atgtctagtc gtgataattc cattccgtta aaaactgttt caagtacatc atcataaagt
     9361 cgtgacgttc tttagcaatc atcttcgccg tatctgtatg cattaaagat tctaacttaa
     9421 gtaatttttc aaagaaatgt ttaattgcag atggtggcaa ctgttcaact aaatcatcat
     9481 taatcttatc tagtgacatt tgttctgtcc acataggttc accaaagtgt cctgcaaatt
     9541 gaaatgttcg tgcaacacct atagcgccta tagcatcaag acgatctgca tccctgacaa
     9601 tttgaccttc taaagataaa gtgacatgat cgtttttacc attgcgatag ctcatattat
     9661 taataataaa taaaatgtgc tcttgatctt cggttgatag tgataaagaa gataaaaatg
     9721 acttcaattc aacatattgt ttgttagcat ctacaacttt ttcgtcaacg gtatcatgaa
     9781 gtaaacatgc gagttcaatg actaaagtat cattaacacc ttcattttca gcgattgatt
     9841 tagctaaagc agtgacacga tatacatgcg caatatcatg gcctgtaaaa tcattttgat
     9901 gaatagattc catatatttt ttggctgcac taatgatgtc tgattgttgc attaaagtta
     9961 cactcctcat atttctattt tataagatag gtgacaataa ttgtgaaata ccttctttaa
    10021 atttaatcca aagacttcgc ttagcataac gtgcttttgt taattcagaa gatactgcta
    10081 aatcatctat aaaagcttgt tttaattttt tggcaatttg ttggtcataa ataaaggcgt
    10141 tgacttcgaa attcaacgtg aaactgcgat ggtccatatt agctgttcca acacttgcaa
    10201 tttcatcatc tataacaagt gtttttgagt gtaaaaagcc attgtcgtag tgaaatactt
    10261 taacaccggc atctaataag gatgctgcat tttttaaagt agcccaaaaa acaaacggat
    10321 ggtcaggttt attaggaatc atgatattga catcaacacc acctaatgcc gcaattttaa
    10381 tagaatctaa aaaggcttga tcaggtatga aatagggaga ttgaatataa atcgattttt
    10441 tagcagatga aatcattttc aagtaaccat atttaatctg ttcccattct tcgtcaggac
    10501 cactagaagc tatttgtacg ccaatcgttc cgccagaatt tacatctggg aaataacgat
    10561 catcatagga gatgtggtca cgtgtggctt gtgaattcca atctagaata aatcgtaatt
    10621 gcaatgcatt cactgcatcc ccgacaattc gtaaatgcgt atctcgccaa tagccgaatt
    10681 tttttgattt acctaagtac tcatcaccaa cattaaaacc accaacatat ccaatttgtc
    10741 catctattac aacaattttt cgatggtttc gattgttcat acgcaagtta attaaaggta
    10801 attttgatgg gaaaaatgct tcagcatgtc cacctttatt gcgaaacggg tgtaagcctt
    10861 ttttacgcag tccacgagaa cccatgtcat cataaagaat tttaacttct acaccttgtt
    10921 ccgctttttt tccaagttca tttaaaatgg tacgacctaa ttcatcattt tgaataatat
    10981 agtactgaaa atgaatataa tcagtagcat tacggatgtc ttgtattaag tcatcaaatt
    11041 tttcttggcc gtctgtgtat acttttaaat cgttgtctgt tgttaaaaat gctgcgttat
    11101 tatataacaa catttgaatc atttctttaa attttacaat ttgataattg gaatttgaaa
    11161 agttttcatt ttttaaagcg gctaattgct catcaacgat taactctaat ccttttttat
    11221 cttccttatc aattttgaaa atttggtcgc gttgaatttg tcgtcctaat agtaagtata
    11281 aaatgaagcc gaataaaggc aagaaaacta agactagtaa ccaagcccag atagaattgg
    11341 cagaacgtct ttccatgaaa ataatggtaa aggcgaataa taagtttaaa ataaatgcac
    11401 caataaatat ggaatttaaa ataatattag aatgcttgag tgcaatggat aataactcta
    11461 tcatttgaaa cctcccatcg aaaatcttaa acgagtagta tatcttatta attgtatcat
    11521 gaagcaattt aaatatcatg acaatttagt ttgaaaaaca cactttgtgt tgacgttgtg
    11581 ttttatggga tatagtatag taaggaatgt aaatgaagga gtgaatgcta tgactgaaca
    11641 agataatgca catcattctg aacaaataaa aacgaatctt aaatcacgtt taaatcgaat
    11701 tgaaggacaa gtgagagcga ttaatcgcat gattgaagaa gatgtctatt gtgatgatgt
    11761 ccttacgcaa ataagagcga cacgttcggc gttaaacagt gttgcgacaa agttattaga
    11821 acaacatatg aaaagttgta ttatgaataa agttaatcaa ggtgctcagg aagaggcaat
    11881 ggaagagtta ttagtgactt ttcaaaaatt gattaaagac taaaggagtt taagatgata
    11941 catcaaaata cgatttacac agcgggaatt gaaacagaag aacaagtaag tcaattgaca
    12001 gaacgcattt caaatatgat aggtgttcat caagtgatta ttaatataat agacggtcag
    12061 gtaactgtag cgtatgagac accagcaaat ttgaatagta ttgaaaaaga aatctatgat
    12121 gaaggataca aaattgtatt ttagggtata atgtagagtg cgctatagat tttaattttg
    12181 aaaataaatt aaaaattttg taaatgatgt agtaaaggta tgtcgaatag ggtaagataa
    12241 tattgaaagc gatccaaacg aggtgataat aatgaaacgt cctgaaaaga ttcaaaatgt
    12301 agtcaaacta ttgtcatcat taggtgtgaa tattaaaaaa actaaatctc gtttagacat
    12361 tattaatact ttgccagcat ctaataaagt aagtcacgaa ttaaaataat tgactgtgat
    12421 tgtgaatcac acaccgttaa agctatcgtc ggactattcg atgatagctt ttttgactgg
    12481 atttactaaa atgaaagcaa gtagtatata acaaaatccc tctatagaga ggctgggaca
    12541 taaatcccta aaaaaaacag cagtaagata tttttcaatt aaaaaatatc ttactgctgt
    12601 tctctattta tacaatactt cgtattgaat ggcttcgc
//
LOCUS       DAKSQE010000078        12256 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.078, whole genome shotgun sequence.
ACCESSION   DAKSQE010000078 DAKSQE010000000
VERSION     DAKSQE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12256
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.078"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            56..184
                     /locus_tag="O0V33_001886"
     CDS             56..184
                     /locus_tag="O0V33_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976510.1"
                     /translation="MIKTMSIENMRTYQQRVYNLWIKGVDNVIYCGYQVGKQKIND"
     gene            complement(329..2257)
                     /locus_tag="O0V33_001887"
     CDS             complement(329..2257)
                     /locus_tag="O0V33_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCX8976511.1"
                     /translation="MILLQLNHISKSFDGEDIFNDVDFEVKTGERIGIVGRNGAGKST
                     LMKIIAGVENYDSGNLSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDWLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLHGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPISIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLQLALLMLQRDNVLILDEPTNHLDIDSKEMLEHALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHEKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDS
                     EQKLEHAMSKWEELQQKL"
     gene            2510..3145
                     /locus_tag="O0V33_001888"
     CDS             2510..3145
                     /locus_tag="O0V33_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCX8976512.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            3451..4530
                     /locus_tag="O0V33_001889"
     CDS             3451..4530
                     /locus_tag="O0V33_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HCX8976513.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITVQSIGLLILTTTGILQIPAHSFPILGTVIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIAIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGAREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVQQQSAKVQQQTQIV"
     gene            4590..4814
                     /locus_tag="O0V33_001890"
     CDS             4590..4814
                     /locus_tag="O0V33_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCX8976514.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPVTNYEQIDNASWTITVQKV"
     gene            5023..6273
                     /locus_tag="O0V33_001891"
     CDS             5023..6273
                     /locus_tag="O0V33_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCX8976515.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGEGNLWFGNWEYTFLNHVGFATQEDISPHIPLALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDQIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAFFQSKKAN
                     PDIENGFIYTGDVHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGL
                     DKIVHGEHAYFEGELNRFNKHIRY"
     gene            6457..7407
                     /locus_tag="O0V33_001892"
     CDS             6457..7407
                     /locus_tag="O0V33_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCX8976516.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTKIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            7556..9040
                     /locus_tag="O0V33_001893"
     CDS             7556..9040
                     /locus_tag="O0V33_001893"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCX8976517.1"
                     /translation="MTEWTREERYQRIEDVDTDYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYGEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNVDNQDVILICPQGIEPKCDQFNNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILETPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            9037..9996
                     /locus_tag="O0V33_001894"
     CDS             9037..9996
                     /locus_tag="O0V33_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCX8976518.1"
                     /translation="MRRLFSIGEALIDFIPNITNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAHMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
                     VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYKVKSVDTTGAGDAFIGAVISRILATDVLN
                     LTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
     gene            complement(10370..11086)
                     /locus_tag="O0V33_001895"
     CDS             complement(10370..11086)
                     /locus_tag="O0V33_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCX8976519.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(11105..>12256)
                     /locus_tag="O0V33_001896"
     CDS             complement(11105..>12256)
                     /locus_tag="O0V33_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCX8976520.1"
                     /translation="VYFFDKIRSISLVVITIFIIIFLYFKIRLYSVFLVMVTQIILYC
                     ANFVYIIIFSYIITISHSVFIVLPIFLVVYVSISYALAYILNRILKRINGTYLSLNKK
                     FLTVITIVIVITFSLLFAYSQIDASDASTIKQYSLLFLGIIILLSILIFIYSQFTLKE
                     MKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMTGLRDYFN
                     KNIVPMKDNLQMNALKLNGIENLKVREIKGLLTAKILRAQEMNIPISIEIPDEVTRIN
                     LNMIDLSRSIGIILDNAIEASSEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHEL
                     FQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN"
BASE COUNT         4159 a         1731 c         2179 g         4187 t
ORIGIN      
        1 tattcttcaa taatattatt attatttcat aaagaaaatg tcttaacaat ggattttgat
       61 aaaaacaatg tcaatagaga acatgcgtac ttatcaacaa agagtgtata acttgtggat
      121 aaaaggtgtt gataacgtca tttattgtgg atatcaggtg ggaaaacaga aaataaatga
      181 ttgattagtg ttaatttact tgtggataac taggataact ctgtggatag ctataatatc
      241 agcgttttta ttgtgaataa aaaaatttaa aatttgtaaa aaaataactc taactatagc
      301 gattttacag ttagagttat gtatattttt atagtttttg ttgtaattct tcccatttag
      361 acatagcgtg ttctaatttt tgttcgctat cttgtttttg gatagctaat tcgtttgctt
      421 tttgtggatt gttataaact tctggttggg ttaattgctc gtctatttgt aagatagtag
      481 tctctaaagt ttctatttca ttttcacact cggatatttg tcgttcaagt ttgcgttgtt
      541 cacgtctttg ttctttttgg ttatgatacg acgattgttc catggacttt tcatgtgctt
      601 gtacgttaac tgactgttct tcttggtgcg ctttcaacgc ttccgcttcc tcaacttttt
      661 caatgtaata ttgataatct cctaaataca tctttccgcc atcaattgtt aaatcaaata
      721 ccttgtttgc taattggtta ataaagtaac gatcatggga aacaaatagt attgttcctt
      781 caaaatgttg gagtgcatgc tctaacattt cttttgaatc tatatcgagg tggttggtag
      841 gttcatctaa aataagtaca ttgtcacgtt gcaacataag tagtgctagt tgtaaacgtg
      901 ctttttcacc accagataaa tcattaatta tctttttaac atcgtcttgt acaaataaga
      961 aacgtccaag aactgcacga atatcttttt cattcattaa cggatattga tcccacacat
     1021 aatctaaaat tgttttacta gatttaaatt ctgcttgctt ttgatcataa taaccaattt
     1081 gtaaatttgc gccaaaagta atatcgccat taagcgcttt ttgttgattt gcaatagttt
     1141 taattaaggt cgattttcca ataccatttg gcccaatgat tgctatatga tcgcctttag
     1201 agacctctat actgataggt ttggtaattg cagtttgata accgatttct aaatttttta
     1261 catgcatgac gtcattacct gtattccggt caaagccaaa ttgaatattt gcacttttgg
     1321 catctaacat tggtttatca atgcgttcca ttttttctaa aatcttacgt ctactttttg
     1381 ccattccgct tgttgaagca cgggtaatat ttttctcaac aaaagtttct aatcgtttta
     1441 tttctgcttg ttgactttca tattcttgca ttcgtttttg ataatataaa tcccgttgct
     1501 gtataaattc ctcgtaatta ccaacatagc gtttgacatc acctaaagcc acatcataaa
     1561 tttgagtaac tattttatct aaaaagtaac gatcatggct gatgatgact attgcacctt
     1621 taaaataacg tagataatct tcaagccact ttgtcgtttc taaatctaag tggttagtag
     1681 gttcatctaa aagtaataaa tcaggttcat ttaatagcat ttgagctaaa gataaacgtg
     1741 ttttttggcc accgctaaaa tcattgatag gtttattgaa atcttcttca ctaaaattca
     1801 acccgtgaag tactgtttta attttacttt catattgata tccttctaat tgttcaaatt
     1861 gattcgataa agattcataa cgggacatat gtgttttata tgtatcacta tcgtaatcat
     1921 ttgcatgttt tgataaccaa tctgtttctt ctttgattaa gctttccatt cgtttaatat
     1981 gttcaaatgg ttttgacatt tcttcgaaaa ctgttgcatt agaatttaaa gtcatttgtt
     2041 gagttaaata acctagtttt aagtttttaa tttttgacaa atttccagaa tcatagtttt
     2101 ctacgccagc tataattttc atcaatgttg atttaccggc gccatttctt cctacgatac
     2161 caattcgttc accagttttt acttcaaaat caacatcatt aaatatatct tcaccatcga
     2221 acgattttga tatatgattg agttgtaaaa gtatcattcg atcttcacct ttcgtctttc
     2281 gcaaatttta ttaacttatg ataaaagtcg taattattac attaataacc attttacagt
     2341 ataaaacgcc gtcttgaaac taagatattt atttttaaaa ttcaataatg tataatattt
     2401 aggtattcat tgtaatcaat tatttgtttt ttaataatct aacaattacg ctctaactcg
     2461 tttgaataca tagatataat tcattcaaac acgaaggagg aaatagcgaa tgagtgacca
     2521 agttaaaatt cctcgagcaa ctttaaaacg tttgccgtta tattatagat ttgtcagttc
     2581 attaaaatct aaaggtatag atcgtgtaaa ttcaaaagcg attagcgatg cgttacaaat
     2641 tgactcggca acaattcgtc gtgacttttc atattttggc gaattaggta aaaaagggta
     2701 cggatataat atagatagtt tattggattt ctttaaatct gaactaagcg agagtgacat
     2761 gatcaaaatc gcaattgtcg gagttgggaa cctagggaaa gctttgctca catataactt
     2821 ttcaatacat gacgatatga cgattacaga agcgtttgac gtaaaagaag atgttattgg
     2881 ccagaaaata gggaacgtta ttgtaaaaga taacgatgaa ttaataacaa cattgaagaa
     2941 ggaagaaata gatgttgtga ttctaactac accagaaaga gttgcacaga aagttgcaga
     3001 tgaactcgtc caagctggtg tgaaaggtat tttaaacttc actcctggta gaataaatac
     3061 gccttcagat gtgcaagtac atcaaattga cttaggtata gaattacagt cattattatt
     3121 ctttatgaaa aattacagtg aataaatgtg atgttagctt tgaatgatta accttgttat
     3181 atctttaatt aattatagaa cagtctggga ctaagtatgt ctcaggcttt ttctgttatg
     3241 tttgaaaatg aaagggaaaa ctgttgtgtt gataaatata atataggtta ataaattaac
     3301 acgtagagat ataatgatga atcgtaatta atcgcatttc ttatatgaac ataatataaa
     3361 tatcaaaaat attgtgtata aggttgaaaa caagaaaatc atcgcctata atacatacta
     3421 aacttatcgg aaattagggg ggttaggatt atgtggatga tttttagtgg cttaattgtc
     3481 gggggactct taggttttgt gatgcaaaga acaagatttt gtttaacagg tggctttcga
     3541 gatatgtatg tgcaaaagaa taataagatg ttctatgcat tattaatcgc tattactgtt
     3601 caaagtatag gattattgat tttgacgaca acaggtattt tacaaattcc tgcacatagt
     3661 tttccaatat tgggaacagt tataggttct tttatttttg gaattggaat agtattggct
     3721 ggaggatgtg caacaggtac ttggtatcgc gctggtgaag ggctaattgg tagttggatt
     3781 gcattagtat tatatgctgt tactgcagca atcactaaaa cagggatttt aaagccagta
     3841 atggataaaa ttaatcaacc aacgaatgta aatagtgata tgtctcaaac aacgggcatt
     3901 ccgttttggg gattagtcgt tatattaact ataatcacca tttttctcgt tgtaagaacg
     3961 ctcaataata agaaagttcg agttgctgtt ccaaaactaa agcaacgtta tacaggtatt
     4021 agatattacc ttttcgaaaa acgataccat ccatttattg cagcaattgc aattggactt
     4081 atcgcactct tagcttggcc aatgagtgca tcaactggaa gaaatgacgg tttaggtata
     4141 acaacgccat cagcaaattt agtacacttt ttgattacag gtgaaactaa atttattgat
     4201 tggggtgtct ttttagttct aggaattttc attggttcat atattgcagc tagaggagca
     4261 agagaattta aatggcgatt gccagacaag attacaatac gaaacagtgc tattggtggt
     4321 atatgtatgg gatttggtgc gtcagttgct ggtggttgtt ctatcggtaa cggtttggtt
     4381 gaaacggcaa cgatgacttg gcaaggatgg attgcgctag catcgatgat agttggggta
     4441 tggacaatga gtcattttat ctttgttcgt ccaatgaaaa aagtacaaca acaatctgca
     4501 aaggtccaac agcaaacgca aatagtatag aaggttatta tgccaatgat gttgatcaaa
     4561 taaaagtgat tggaaaagga gaaatagcta tgatacacga attaggtaca gtaggaatgg
     4621 tatgtccatt tccgttaatt gaagcgcaaa agaaaatggc aacattgcaa tctggagatg
     4681 aattaaaaat tgattttgat tgcacacaag cgacggaagc tattccaaat tgggctgcag
     4741 aaaatggata tcctgtaaca aactatgaac aaattgataa tgcttcatgg acaattacag
     4801 ttcaaaaagt ttaacgttat cattttaata ataaaattga tattagattc tatggctact
     4861 tccgctactt taaaagcgag taagtagtct tttttgtttt agttcatgaa atcatttttt
     4921 atatagcgtg gcacattttg ttccaaaaga tgtaataaaa ctttacgcat ttttgctttt
     4981 tataaattat cagattatta tggaaacaag ggagtggtaa gtatgaatct taacgatacg
     5041 atatttatgt ttttgtgtac attattagtt tggctaatga caccaggatt aagtttattt
     5101 tatggtgggt tagttcaatc taaaaatgcg ctaaatactg tcatgcaaag tatggcagca
     5161 attgtgcttg ttacatttgt atggataaca gtaggtttta caattagttt tggggaaggg
     5221 aatttatggt ttggtaactg ggaatataca tttcttaatc atgtaggttt cgcgacacaa
     5281 gaagatatta gtccgcatat tccattggca ttatttatgt tattccaaat gatgttttgt
     5341 acgattgcaa tctcaatatt atcaggttca atcgctgaga aaatgaagtt tattccttat
     5401 ttattatttg tagtaatatg gactgctctt gtatacagtc cagtagcaca ttgggtttgg
     5461 ggcggcggtt ggattaacaa actcggtgta ttagatttcg ctggaggtac agtcgttcat
     5521 attacatcag gtgtttctgg tttagtatta gctataatga ttggaaaagg aaacaaacat
     5581 tctgaatcaa caccacataa tcttatcatt acattgattg gcggtatatt cgtgtggatt
     5641 ggttggtatg gatttaatgt aggcagtgct tttacatttg atcaaattgc aatgcttgca
     5701 tttacgaata cagttatttc tgccagtgcc ggggctattg gttggttaat tttagaatat
     5761 atttttaaaa agacgacaag tttacttgga cttttactcg gtgcattagc aggattagtt
     5821 gttattacac ctgcagctgg atatgtaaca tatcttagtg caacaatcat ggctttaata
     5881 gggggtatct gttgttatat tgtcatcaat tacataaagg taaaattaaa gtatcatgat
     5941 gcattagatg catttggtat ccacggtgtt ggcggtatta ttggtgcagt attaacagcc
     6001 tttttccaaa gtaaaaaagc aaatccagat attgagaatg gttttattta cactggtgac
     6061 gtgcatatta tacttgtgca aatattatgt gtaacagcag ttgtaatttt tagcatcgtc
     6121 atgacgttta ttattgcgaa agtaattaaa ttaattacac cattatctgt tacagaacaa
     6181 gaaacgaata tagggttaga caagattgtt catggtgaac atgcttactt tgaaggtgag
     6241 ttaaatagat tcaataaaca tattcgatat tagatttata tatacataga atattcattg
     6301 tcctgacatt taactaaagg ttgatgttgg gacattttgt tatacagaaa ttttattttg
     6361 aaatcttttt atgaaagaag cagaaatatt atttaaagcg gttacacata tgctaaaata
     6421 aggctaagtg tcacaaataa tgataggtga ataagtatga aaaatatatc tgatattgcc
     6481 aaattggcag gcgtttcaaa aagtacagta tctagatttt taaataatgg atctgtcagt
     6541 aaaaaaacaa gtgaaaaatt aacaaaaatt atagcagaac atgactatca accgaatcaa
     6601 tttgctcaaa gcttaagagc gagacaaaca caccttattg gtgccattat acctagaatg
     6661 aattcatatg cagtagatga aacaatcaaa ggattggcaa aacaatgcca aaaatatgaa
     6721 tcacaattaa ttttaaatta tacaggttta aatatcgaag cagaaataca agcgcttgaa
     6781 acattagcac gcagtaaagt agatggtatt gttttaatgg ctacagacat aacagagaga
     6841 catattgaag tcattaataa aatgaatgta ccaatcgtta ttgttggtca acaacatgaa
     6901 caactgcata gtattgtgca tgatgattat aaagcaggtc aaattatagg tgaatggatt
     6961 ggtcaacagg gatatcaaca agttgaagtg tttagtgtga gtgaaaaaga tattgcagtt
     7021 ggtatacata gaaaacgtgg tttacttgac cagttagcca aatatcaaat taaacctaat
     7081 atacatgaaa caaattttac ttatgtggaa gcacaaaaag atgttgcaaa tgttttggaa
     7141 aatgtagagc aagtagatgc ggttgttgga gcaactgata cgattgcatt agctgcctat
     7201 aaatattatt ctgataaaaa agatgttatg aaaccacatc aaatatatgg ttttggtggt
     7261 gatccaatga cacaattagt gtctccatcg ataaaaacaa ttcattataa ttattttgaa
     7321 gctggtcaat gcgcgatgga agagatacaa cagatgctta aaaagcaaga tatgccatat
     7381 agcgtcacag tagatgttaa tatttagacg ctgtattttt taaaataaat gtggaaccga
     7441 taccataaaa gtataaatgg ataggttaaa agttaaagaa cgtaggtaaa atttgctata
     7501 atagaatata aattgttaac agcataaatt ataaaaggag gactgggtga atattatgac
     7561 cgaatggact agagaagaac gttatcaacg aatcgaggac gttgatactg attattttaa
     7621 aacattaaaa caacaagttg atcaatcaaa atttcgtcaa caatttcata tacaaccaga
     7681 aacgggctta ttaaacgatc ccaacggact tattttttat aaaggaaagt attatgtttc
     7741 acatcaatgg ttcccattag gcgcagtaca tggcttaaag tattggtata actacacgag
     7801 tgatgactta ataaacttta aagctgaagg gccaatttta aacccagata ccaaatatga
     7861 cagccatggt gtatatagcg gtagcgcttt tgaatataac gggcatttat attatatgta
     7921 cacaggaaat catcgagata atcattggca acgccatgcg agtcagatga tcgcacgatt
     7981 gaaagaagac ggttcagttg aaaagtttcc aaagccagta attagccagc aaccagaagg
     8041 atatacgagt cattttagag atcctaaagt ttttaaatat ggtgagaaat attatgcgat
     8101 cattggcgca caaaataatg atcagcaagg tcgattatta ctttataata ctgaagatat
     8161 aattaattgg cattatttag gtgaaataaa tacagagtta gatgattttg gatatatgtg
     8221 ggaatgccct gattacttta atgtagataa tcaagatgtc atacttattt gtccacaagg
     8281 tattgaacca aaatgcgatc agttcaacaa tatttatcaa agtggttata tacttggaaa
     8341 gtttgatatt gaaaagttaa catatgaaca tgaaaatttt gtcgagcttg ataatggttt
     8401 tgatttctat gcacctcaaa catttttaga tgaaaaaggc cgacgagtac taattggatg
     8461 gatggggtta ccggaaatcg aatatcctac agataatgaa ggatgggccc attgcttaac
     8521 aattcctcgt gtattaaatg tagaaaatgg tcaacttaag caacgtccat atccagcgtt
     8581 ggaaaaatta cgtcacaata aagagacagc attaggctac gcaaataaat ttactcgaaa
     8641 attacatccg tatgaaggta aacagtatga attaatcata gatattttgg ataatgatgc
     8701 taccgaagta tactttgaat tacgtacgtc taagacttct tcaacattaa tcgcttataa
     8761 caagcgtgaa aataaaataa cattagatcg cagcgacagt ggtttattgc ctacaaatgt
     8821 tgaaggtacg acgcgtagta cgatattaga aacgccatta aagcaattac aaatttttgt
     8881 tgatacatct agtatagaaa ttttctgtaa tgatggggaa cgtgttttga catctcgaat
     8941 tttcccgaca gaagacgcac taggtattaa aacatcaaca gaatcagggc aagtatattt
     9001 acaatttact aaatatgatt taaaggatga acataaatga gacgtttatt ttcaatagga
     9061 gaagcgttaa ttgattttat tccaaatatt acaaatgcga atttaaaaga tgttcaaaca
     9121 tttacgaagc aaattggtgg cgcgccatgc aatgtagctt gtacagttca aaagttagga
     9181 caacaagcgc atatgattac acaattaggt aatgatgcat ttggagatag cattattgaa
     9241 acgatatcaa gtattggtgt agatgtatca aaagtgtatc gcacgaatga agcaaacact
     9301 gcgttagctt ttgtgagttt gactgaagcg ggagagcgag atttttcttt ttatcgtaag
     9361 ccctctgctg atatgttatt tgaaccaagt tttgtgaatg atattgatgt gaatgaaaac
     9421 gatgtagtac atttttgttc tgttgatttg gtcgatagtc caatgagaga tgcgcattat
     9481 caattgatta ccaaaacatt aaatgcaaat ggtactgtcg tatttgatcc taatgtaaga
     9541 ttgccattat gggataacgc agaagattta agacaaacta ttcatacctt tttgccattg
     9601 gcgcatattg taaaagtttc agatgaagaa ttagaattta ttacaggtat tcatgatgaa
     9661 aatgaagcca ttcaatcatt atttacaggt aatgtcactg tagtcattta tacgaaagga
     9721 gcagatggtg ctgccgtata tttgaaaaat ggtattaatc attatcatag tggctataag
     9781 gtaaaatctg ttgatacaac tggggcaggg gatgccttta ttggtgcagt tattagcagg
     9841 attttagcaa ccgatgtgtt aaatttaact cagttatttg aaaatgaagg tgaggaaatt
     9901 ttagcgttta gcaatcgcgt tgctgcaata gtgacaacga agtatggtgc tattaatagt
     9961 ttgccaacat tacaagaggt tgaacaagca ttttaaataa ttcatttata aaacagtgta
    10021 ggaaacaagt tgaatcttgt gctacactgt ttttttgcga gcaaaataag atttattggg
    10081 taggatattg tagcatttta tttatggaaa ctgtcgattt caaatttagt atattttgtt
    10141 aaaaattgcg ctataggatt gtagagtgaa atagaaattg ataaattatg tagtaatagg
    10201 aataggtata caatgaaacg ttgtatcgtt ttatttaaat ttacgcttta aattaaattt
    10261 ttgctattaa aaaataaaaa gccagctata cagtgcattt gctagttatc ttgttaaaat
    10321 ccaacaagat ttacaattga atacgccgtt aactgacttt attatcttat tatatttttt
    10381 taacgtttct caccgatgca tagcagtgtt ctttattttt aaaataaaca atacgctctt
    10441 tcgaatcaat agattcgata ttatgacgat tgacgacaaa gctattatga catctgaaga
    10501 aacgatcatc taattgactc agttctttta aattaccata aaattcaatt tgacggttat
    10561 ctaaatgggc aatgagtctg tgagattttg ttgatgattc aaaaaacata atatcatcat
    10621 attgaacata cactgaatta ctgccacgtt ttaactcaat cgtttcaacg ctattatctt
    10681 ttgataataa ttgtaagcgt gtatgtgcag tttctaaaca atctataatt cgagttctta
    10741 attctgctgg atcatcttta aaaataaaat ccatcgctgc aactttgtag acaaatgtta
    10801 aatacgtaag ttcactgtga ctcgtaacga aaataatgtt accaactggg tcatgcttac
    10861 gaatttcact gcctaatttg ataccattaa tatcagttga aagttgaata tctaaaaagt
    10921 aacagcctat gtcattcata tttttagctt gctcaagcac ctcataagga ttatcagttg
    10981 cgagggcaat ttccataggc ttttcttcta tcattatata atttttgata atggtaacca
    11041 tgttttctct ttgttttgga tcgtcttcgc aaatgaaaat tttcatacat tcacatcctt
    11101 atggctagtt gttaataatt tcaacttttt gaatgaagaa accattttcg ataattgtat
    11161 ctaacaagac attgtctgca ttatcagcaa tttcttttaa agttgataga cctaaaccac
    11221 gaccttcacc tttagtagaa aaactttctt ggaacaattc atgaatacgt ggtatatcat
    11281 ctgcacattt attcataaca ataaacgtta ctgaattttc gctttcaata aatgcaactc
    11341 gaatgatagg atcatcaatt tcacttgatg cctctattgc gttatcaaga ataataccaa
    11401 tactgcgact taaatcgatc atattcaaat taatgcgagt tacttcatct ggtatttcga
    11461 tactaatcgg tatattcatt tcttgtgcac gtaaaatttt agctgttaga agacctttaa
    11521 tctcacgtac tttaagattc tcaataccat ttaacttaag agcattcatt tgtaaattat
    11581 ctttcatagg tacaatattt ttattgaaat agtctcgaag accggtcatg tcatcttcac
    11641 gaatgtattc tgaaagagtc gttaagatat tgacataatc atgacggaac ttgcgcattt
    11701 cattattaat tgcttcaatc tttaatgtgt attcataata ggtttcaatt tcttcttgat
    11761 tacgtttata tttcatttct ttaagagtaa attgagaata aataaaaatt aagatactca
    11821 gtaatataat aattcccaag aataaaagcg aatactgttt gattgttgat gcatcactcg
    11881 catcaatttg cgaatatgca aataacaaag agaatgttat tactataaca atagttataa
    11941 cagtaaggaa ttttttatta agagaaaggt atgtaccatt tatccttttt aatattctat
    12001 ttaaaatgta ggcaagtgca taagaaattg atacatacac taccaaaaag attggtagaa
    12061 ctataaacac gctgtgtgaa attgttataa tgtatgaaaa aataatgata taaacgaaat
    12121 ttgcacaata taaaatgatt tgagtaacca ttactaaaaa tacagaatat agtctaattt
    12181 taaaatagag aaaaattatt ataaaaatcg ttataacaac tagtgaaata cttctgattt
    12241 tgtcaaagaa atatac
//
LOCUS       DAKSQE010000079        12160 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.079, whole genome shotgun sequence.
ACCESSION   DAKSQE010000079 DAKSQE010000000
VERSION     DAKSQE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12160
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.079"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(112..219)
                     /locus_tag="O0V33_001897"
     CDS             complement(112..219)
                     /locus_tag="O0V33_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976521.1"
                     /translation="MVLLAFVTTIHDTDDGRFFNEHKHANNKLLKRSLI"
     gene            complement(256..579)
                     /gene="isdG"
                     /locus_tag="O0V33_001898"
     CDS             complement(256..579)
                     /gene="isdG"
                     /locus_tag="O0V33_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HCX8976522.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(598..1332)
                     /gene="srtB"
                     /locus_tag="O0V33_001899"
     CDS             complement(598..1332)
                     /gene="srtB"
                     /locus_tag="O0V33_001899"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HCX8976523.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQAHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKILNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDN
                     KYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDKIM
                     TLSTCEDAYSETTKRIVVVAKIIKVS"
     gene            complement(1394..2359)
                     /gene="isdF"
                     /locus_tag="O0V33_001900"
     CDS             complement(1394..2359)
                     /gene="isdF"
                     /locus_tag="O0V33_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HCX8976524.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLFTKFTTG
                     SNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            complement(2372..3250)
                     /gene="isdE"
                     /locus_tag="O0V33_001901"
     CDS             complement(2372..3250)
                     /gene="isdE"
                     /locus_tag="O0V33_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HCX8976525.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            complement(3247..4323)
                     /gene="isdD"
                     /locus_tag="O0V33_001902"
     CDS             complement(3247..4323)
                     /gene="isdD"
                     /locus_tag="O0V33_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HCX8976526.1"
                     /translation="MRNVKQIATKSIIAIISLGILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTSSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KDLDNKEMLYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDIKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTVKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFA
                     VGIVLATILLISAHLYSRKRGNQV"
     gene            complement(4323..5006)
                     /gene="isdC"
                     /locus_tag="O0V33_001903"
     CDS             complement(4323..5006)
                     /gene="isdC"
                     /locus_tag="O0V33_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HCX8976527.1"
                     /translation="MKNILKVFNTMILALIIIIATFSNTANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKERIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPSDVAGANAPGKDDKN
                     SASGSDKGSDGATTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
     gene            5215..6279
                     /gene="isdA"
                     /locus_tag="O0V33_001904"
     CDS             5215..6279
                     /gene="isdA"
                     /locus_tag="O0V33_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HCX8976528.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKNITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQAVLNNASFWKEYKFYNANNQELATTVVNDDKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEK
                     VKPAVTAPSKNENRQTTKVVSSEATKDQSQTQSARTVKTTQTAQDQNKVQTPVKDVAT
                     AKSESNNQAVSDNKSQQTNKVTKQNEVHKQGPSKDSKAKELPKTGLTSVDNFISTVAF
                     ATLALLGSLSLLLFKRKESK"
     gene            6481..8439
                     /gene="isdB"
                     /locus_tag="O0V33_001905"
     CDS             6481..8439
                     /gene="isdB"
                     /locus_tag="O0V33_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HCX8976529.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAKAAEET
                     GVTNTEAQPKTEAVASPTTTTTEKAPEAKPVAKPVANAVSVSNKEVVAPTTETKEAKE
                     VKAVKEVKAPKEAKEEKPAAKADNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDF
                     EMKKKDGTQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKL
                     VSYDTVKDYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKT
                     EEDYKAEKLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEETKKALDEQVKS
                     AITEFQNVQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVME
                     TTNDDYWKDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQ
                     YHVRIVDKEAFTKANADKTNKKEQQDNSAKKETTPAMPSKPTTPPVEKESQKQDSQKD
                     DNKQSPSVEKENDASSESGKDKMPVTKPAKAEVESSSTTPTKVVSTTQNVAKPTTASS
                     ETTKDVVQTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEES
                     NKDMTLPLMALIALSSIVAFVLPRKRKN"
     gene            complement(8593..8766)
                     /gene="rpmF"
                     /locus_tag="O0V33_001906"
     CDS             complement(8593..8766)
                     /gene="rpmF"
                     /locus_tag="O0V33_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCX8976530.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(8846..9403)
                     /locus_tag="O0V33_001907"
     CDS             complement(8846..9403)
                     /locus_tag="O0V33_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCX8976531.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            9530..10669
                     /locus_tag="O0V33_001908"
     CDS             9530..10669
                     /locus_tag="O0V33_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCX8976532.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVSDYMSVDTIAFGSE
                     NNNIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKHAKNINAISIKRENAQHHDSLIQHHKFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTESLKNIYTVTEGFENRLKSYINEATDFHHFV
                     KSLKTKRYTYTHIQRLLMNVLLNIKPTDVTRNIHAVKVLAMNDRGRQYLKHLKTVFPE
                     RQYITNINKSNAHYFTNEIKTTHIYNAISGQQQTDFNTPVIQQYR"
     gene            complement(10731..11213)
                     /gene="coaD"
                     /locus_tag="O0V33_001909"
     CDS             complement(10731..11213)
                     /gene="coaD"
                     /locus_tag="O0V33_001909"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCX8976533.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(11215..11757)
                     /gene="rsmD"
                     /locus_tag="O0V33_001910"
     CDS             complement(11215..11757)
                     /gene="rsmD"
                     /locus_tag="O0V33_001910"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCX8976534.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGIDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            11827..>12160
                     /locus_tag="O0V33_001911"
     CDS             11827..>12160
                     /locus_tag="O0V33_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976535.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLY"
BASE COUNT         4279 a         2141 c         1628 g         4112 t
ORIGIN      
        1 ttttatcgat actactacta taaatcatac gccccaaaat atcattatta attcttttct
       61 tcttcaaaat aaatcaaaat catatatttg atgattattt tcaaagcaca ttcatatcaa
      121 actacgtttt agcaatttgt tgttagcatg tttgtgttca ttaaaaaaac gaccatcatc
      181 ggtatcatgt atggtcgtta caaaagctaa caataccaat tgtcataaca agtactgcaa
      241 cctttttaaa ttcaattatt tcatgtaact atagcctata tcatatgtaa ttactttgtt
      301 attaataatc gggctacttt catcttcatt tttacttcta acatgtttat gtgctgcttt
      361 aaagacatca gattttaacc aatccgtaaa agcttgcttt gatttccaaa ctgttaaaat
      421 tttcacttca tcaaaatctt cttgttctaa agtttgtgta acaaacatgc catcaaagcc
      481 ttctaatgtt tcaatcccat gtctcgtgta aaatcgttct ataatatctt ttgctgttcc
      541 ttttgttaac gtcagcctat tttctgccat aaatttcata attatcctct tttctgttta
      601 acttacctta attatttttg cgacaacaac aattcttttc gttgtttcac tatatgcatc
      661 ttcgcacgtt gataaagtca ttattttatc ttttaccgtt acattaacat ctgagttaat
      721 tacagattta cgttttgtct catctaaaaa ttgttgataa tcttgatcat tttcaaaatc
      781 tgtacgtatg taattatctt tagtagtagt tttatatgca ctaaatactt gcaattgata
      841 tttaccatat ttattgtcaa attcaattat cttgtgtttt tcataaaaag attgctttaa
      901 ataatcttct aacacatcaa acatcgtatt atcaccgaca tggtgcccgt ataaaatagt
      961 attatgattt aaaatcttca attcatttct aaaatccata aaaatactac ctttacgtcg
     1021 atgttctcgc tcaaaatcta aatttaaata atcgtgattt gtcttacctt gtagtactgg
     1081 ataattcaat gatgttcctg ataattttat ccatccaaca atgtctttat ttattttttc
     1141 aagtgattca aattgtggtc tcacatgtgc ttgatgtttg ctcatcaaca tttgaaattt
     1201 ttgttgtaat ttctcataat ttgcgcgttc ttgcttgtct tcaatatatg tttgaacaat
     1261 tttgtaacca aaaatgataa taattacaac caataaaatt tgtacaatag ttaaaaatcg
     1321 cttcattctc ataaaaatcc tcttttatta acgacgtttc ttcagtcatc actaaaccag
     1381 ttgttgtacc gttttagatt cgatttcgtt gactttgaca aattaagtaa attagcattg
     1441 gaccaccgac aatcattaaa atagcattgg ctggaatttc taaaggaggc tgtatcactc
     1501 gtcctaataa atcagccact aacaatagcc atgcaccaat aactgtagaa aacggaataa
     1561 gtactctgta attgccccca actagctttc taaccacatg tggcacaata atacctaaaa
     1621 aggctagttg tccaacaatc gcaacagttg cacttgctaa aaatactgct aataaacctg
     1681 ttaaccatct gtaacgatca atattaaaac cgatacttcg cgcttgtatg tcatctaaat
     1741 ttagtaaatt caatttaggg gacaatagta atgttaatat taatcccaat aatgctgata
     1801 ctgctaatat gtatacgtca ctccatattt tcattgttaa gccttgagga attttcatta
     1861 aagggttttg agttaaaatt tctaaaacac catttaataa tacgaataac gcaacaccta
     1921 ctaatatcat actaacagca ttgaatctaa atttagaatg caacaatata attattaaaa
     1981 atggtattaa acctccaata aaacttaata atggtaagta aaagtacaat tgtggaataa
     2041 acaacataca aagtgctctc attataagtg cacctgatga aacgccaatg atattcgcct
     2101 ctgccaaagg attttgtagt gctgcttgta ataatgctcc agaaactgct aacattgcgc
     2161 caaccatcaa tgcaattaat atacgtggca atcgcaaatc aataattgaa tccactgctt
     2221 cattgctacc agttgtaaat tttgtaaata ggtcattaaa tgacaattta attgtaccgg
     2281 ttacaaacga aatataagcc gttgcgatta aaatgactaa caaacataaa aatagtagtt
     2341 tctttttatt ttttatcatc atatcaactc actatttttt atccttataa aataaatcat
     2401 ataattgagt cattgcctta tcagcatcaa catttgctgt aataccgaat ggcacttcct
     2461 ctaagtcata aacatgatta tttttcacag ctttaaaatg tttccaaata tcattttgtt
     2521 taaattcttt ttgaaacatt ttcttaactt cttcaggcat tccgtgtggt aatcgtaaaa
     2581 taatatctgg attgatattc aacaaatttt cagtattaga cgaaatatat tgacgatctt
     2641 tcactttaat aacattttct ccacctgcta ttttaactaa atcaccaata tatgatttat
     2701 cagttgctac taaatagcta ccaggtacac ccattaatat taatactttg ggatgtttct
     2761 tttgtttagc tgctttattt tcaatttttt gctttacaga atttaaatgg tcatttaatt
     2821 cttttgcttg tgctttacga ttaaattgat cacctaattg tgtaatcgac ttttgcatcc
     2881 cttttaaact atcaaaatca taaaagtagc ctttcatatt taattgtttg taaaatggtt
     2941 gcatttcatc tttaatcgtt gacacactca aaacatgtgt tggttttaac tttttaacag
     3001 cttcaacatt cggctccatt ggttgaccaa tttccggtac atctttataa cgattaggca
     3061 atgtcttata tgacgtgggt ttgccgacaa ttggtaaatc caatttgtcc aatgtcattg
     3121 tcaatgcaac agttgttggt acgattctga attcgccgga tttagttgat tcttgagaac
     3181 tggaagattg acagctggtt aagataacga cgcttatcac taaaatggtt aaatacttta
     3241 tgattctcaa acttggttac ctctctttct gctgtataaa tgtgccgaaa taagtaaaat
     3301 ggttgctaac acaatgccaa ctgcaaataa catatattgc tttttatgat ttggttcatc
     3361 aagttcttta atatcactag ataattgact actattcttg ctatcatcat ctctattaaa
     3421 agctggtaac gctttttctt tttcaactac ttttttcttt tccttttttg atttttcatc
     3481 tttatgtttc ggttgctctt tgtgattatt ttcaaccttt tttactgttg gagggctatt
     3541 ttcaactttt aatgtacctg atgctttagg tcgctcgttt tcaatctttt tattactgtc
     3601 ttgttgcact ttatcttttt gtacaggaat atcatttaat cctttttcag taaaaaggct
     3661 agtacgtctt tccttaatgt ctgatttttg actatctttt ttgtcatcaa tatctttttt
     3721 aagctgatct tgattatttt cttgattttg atgttttgaa ggcacttctt ttgcatcgtc
     3781 atgttgtact tttttctctt taaaatgaat agaatacagc atttctttgt tatctagatc
     3841 tttcttttca gccaacgttt taacatgcat attgatttta tccatatgct gttcaattaa
     3901 aattcttact attgtgtcat ccttttcatc ttttataaca aaggggaatt cttcattttt
     3961 gtcattttga aaaacaagtt gttctttatc aatttgacct tttagtttta cttgcaagta
     4021 aattttacca gtcatatctg aaatcaattg cgccttttcg tcaactagtt gttgttcctc
     4081 agttacattt ttattttctt gattaaaaat tagtaattct aacgctttat tctcttttat
     4141 cttttcatca aaaattgatg aagttaattc tgttttatct tttgcttcag gttgattaaa
     4201 tttggctgat ggatatttta tctcatcagc caacacgcta ccaatcattg ttgtatatgt
     4261 aagaatacct aagctaataa tagctataat agattttgta gcaatttgtt taacatttct
     4321 cattattcca cattgccttt agattttttt ctaacaaaca atgtgattgc gattaataat
     4381 gctaaggatg caactggtat tgcatatata tatgcaggtg taccagcatt tgtttgtgga
     4441 ttttctactt tgtctttatt cgaactgtta gattcacttt gaccagtagt cgctccatca
     4501 gatcctttgt cactacctga agcagaattt ttatcatctt tacctggtgc attagcacct
     4561 gctacatcag atggtccatt aaatttatat gtaatattgt aatgatggtc atatttgaaa
     4621 ggttttccgt tcactttttc atcgatataa acatcaattt tcccatctat tttaccgttt
     4681 agcttactta cttcaaattc agaagttcgt tcatctttgg cagtattttt actaataatt
     4741 ctttctttat gtccttctat actcattcct gtaatccaat gactgtggtt tacagttatt
     4801 tgaacataca atttaccatt tttcttaatg tactttgccg gtttattaaa atagtcatta
     4861 gcaatcgacg tgtcattggt attgtatttg taaacctcat aattcaaagt accgctatct
     4921 gcggcatttg cagtattact gaatgtcgcg atgatgataa ttaacgctaa aatcattgta
     4981 ttaaaaactt ttaaaatatt tttcaaaaca taatcctcct ttttatgatt gcttttaagt
     5041 ctttactaaa atcataaata ataatgatta tcattgtcaa tatttatttt ataatcaatt
     5101 tattattgtt atacgaaaat agatgtgcta gtataattga taaccattat caattgcaat
     5161 ggttaatcat ctcatataac aacacataat ttgtatcctt aggaggaaaa caacatgaca
     5221 aaacattatt taaacagtaa gtatcaatca gaacaacgtt catcagctat gaaaaatatt
     5281 acaatgggta cagcatctat cattttaggt tcccttgtat acataggcgc agacagccaa
     5341 caagttaatg cggcaacaga agctacgaac gcaactaata atcaaagcac acaagtttct
     5401 caagcaacat cacaaccaat taatttccaa gtgcaaaaag atggctcttc agagaagtca
     5461 cacatggatg actatatgca acaccctggt aaagtgatta aacaaaataa taaatattat
     5521 ttccaagctg tattgaacaa cgcatcattc tggaaagaat acaaatttta caatgcaaac
     5581 aatcaagaat tagcaacaac tgttgttaac gatgataaaa aagctgacac tagaacaatc
     5641 aatgttgctg ttgaacctgg gtataagagt ttaactacaa aagtacatat tgtcgtgcca
     5701 caaattaatt ataatcatag atatactacg catttagaat ttgaaaaagc aattcctaca
     5761 ttagctgacg cagcaaaacc aaacaatgtt aaaccggttc aaccaaaacc tgctcaacct
     5821 aaaacaccta ctgagcaaac gaaaccagtt caacctaaag ttgaaaaagt taaacctgct
     5881 gtaactgcac caagcaaaaa tgaaaacaga caaactacaa aagttgtaag tagtgaagct
     5941 acaaaagatc aaagtcaaac acaaagtgct cgtacagtga aaacaacaca aacagctcaa
     6001 gatcaaaata aagttcaaac acctgttaaa gatgttgcaa cagcgaaatc tgaaagcaac
     6061 aatcaagctg taagtgacaa taaatcacaa caaactaaca aagttacaaa acaaaacgaa
     6121 gttcataaac aaggaccttc aaaagattct aaagctaaag aattaccaaa aactggttta
     6181 acttcagttg ataactttat tagcacagtt gccttcgcaa cacttgccct tttaggttca
     6241 ttatctttgt tacttttcaa aagaaaagaa tctaaataaa tcttcgtcat actcataact
     6301 taatatattt tttattttaa attttattta acctatgtca tagatattac ataatctata
     6361 acataggtta ttttttataa aataacattg caattaacta ccatttcaat gtacaataca
     6421 agtaatcaat tgataatgat tatcagttga taatatacaa ttaggagttg tttctacaac
     6481 atgaacaaac agcaaaaaga atttaaatca ttttattcaa ttagaaagtc atcactaggc
     6541 gttgcatctg tagcgattag tacactttta ttattaatgt ccaatggcga agcaaaagca
     6601 gctgaagaaa caggtgttac aaatacagaa gcacaaccaa aaactgaagc agttgcaagt
     6661 ccaacaacaa caacaactga aaaagctcca gaagctaaac cagtagctaa accagtagct
     6721 aatgctgtct cagtatctaa taaagaagtt gtggccccta ctactgaaac aaaagaagct
     6781 aaagaagtta aagcagttaa agaagttaaa gcccctaagg aagcaaaaga ggaaaaacct
     6841 gcagcaaaag ctgacaacaa tacatatcct attttgaatc aggaactaag agaagcgatt
     6901 aaaaaccctg caataaaaga caaagatcat agcgcaccaa actctcgtcc aattgatttt
     6961 gaaatgaaaa agaaagatgg cactcaacag ttttatcatt atgcaagttc tgttaaacct
     7021 gctagagtta ttttcactga ttcaaaacca gaaattgaat taggattaca atcagggcaa
     7081 ttttggagaa aatttgaagt ttatgaaggt gacaaaaagt tgccaattaa attagtatca
     7141 tacgatactg ttaaagatta tgcttacatt cgcttctctg tttcaaatgg aacaaaagca
     7201 gttaaaatcg taagttcaac tcacttcaat aacaaagaag aaaaatacga ttacacatta
     7261 atggaattcg cacaaccaat ttataacagt gcagataaat tcaaaactga agaagattat
     7321 aaagctgaaa aattattagc gccatataaa aaagcgaaaa cactagaaag acaagtttat
     7381 gaattaaata aaattcaaga taaacttcct gaaaaattga aagctgaata caagaagaaa
     7441 ttagaagaga cgaaaaaagc tttagatgaa caagtgaaat ccgcgataac tgaattccaa
     7501 aatgtacaac caacaaatga aaaaatgacc gatttacaag atacaaaata tgttgtttat
     7561 gaaagtgttg agaataacga atctatgatg gatacttttg ttaaacaccc tattaaaaca
     7621 ggtatgctta atggcaaaaa atatatggta atggaaacta ctaatgacga ttactggaaa
     7681 gatttcatgg ttgaaggtca acgtgttaga acaattagca aagatgctaa aaataacact
     7741 agaacgatta tcttcccata tgttgaaggt aaaactctat atgatgctat cgttaaagtt
     7801 cacgtaaaaa cgattgatta tgatggacaa taccatgtca gaatcgttga taaagaagca
     7861 tttactaaag ccaatgccga taaaactaat aaaaaggaac aacaagacaa ctcagctaag
     7921 aaggaaacta ctccagctat gcctagcaaa ccaacaacac cacctgttga aaaagaatca
     7981 caaaaacaag acagccaaaa agatgacaat aaacaatcac caagtgttga aaaagaaaat
     8041 gacgcatcta gtgagtcagg taaagacaaa atgcctgtta caaaaccagc taaagctgaa
     8101 gtagaatcaa gtagtacaac tccaactaag gtagtatcta cgactcaaaa tgttgcaaaa
     8161 ccaacaactg cttcttcaga aacaacaaaa gatgttgttc aaacttcagc aggttctagc
     8221 gaagcaaaag atagtgctcc attacaaaaa gcaaacatta aaaacacaaa tgatggacac
     8281 actcaaagcc aaaataataa aaatacacaa gaaaataaag ctaaatcatt accacaaact
     8341 ggtgaagaat ctaataaaga tatgacatta ccattaatgg cattaatagc tttaagtagt
     8401 atcgttgcat tcgtattacc tagaaaacgt aaaaactaat taatcgtctt tatatttaat
     8461 tattaaatta acaaatttta attggcagat gaggtattcc gatacctcgt ccgccaatta
     8521 ttttttgcaa tataaaaagt cccacttaaa acaattattt taagcgggac tttttatatt
     8581 gagtaactaa aattatttag ctgctacttc ttcgccattg taagaaccac agtttttaca
     8641 tacacggtgt gataatttgt attcgccaca gtttgggcat tcagtcatac ctggtactga
     8701 aattttgaaa tgcgtacgac gtttgttttt tctagtttta gaagttcttc tttttggtac
     8761 tgccatgata tatcctcctt agattataaa cgaaaaatac taaatgttag tttaattaac
     8821 aacattatat cattaattaa actacttatt gctctttatc atataattgt tgtaattttt
     8881 gaagccttgg atcaacttgt cgtgattctg aatcatcttg ttcttgctgt ttagcaagct
     8941 catctaattg atcctcatcg attacttccc aaccattacc tactgtcaac atttggtcac
     9001 tttgctctga ataagctctc attggtttct caataataac tatatcctcg acaatatcct
     9061 gaagattaac cataccatct ttaataatgt gatagtgttc atctacatca tcttgatcat
     9121 cgttatactg attgtaccct tctaaatcaa atacttctgt agtagttaca tctagtggga
     9181 ctttaactgg tacaagagta cgtgcacaag gcattgtata cgttccagta atgtgaatat
     9241 ccgcaacgac ttctgttgac ttaatggtta actgaccttg gattgtaatt ggagataaat
     9301 caattaaatc taatgattct tttaaattgt caaaactcac cgtttgatca aattcaaatg
     9361 gcttaccttg atatttcctt aattgcgtaa ttgaccattt catatggctt cacctctaac
     9421 aagcacaaaa tttattttaa ctttctaaga acaagttgtc aagatttttt cttaacatat
     9481 catgttctgt tacaatatta ataatgaata ccgaaaggac gaattgcaaa tgaaaagcgt
     9541 tggcttaatc acagaatata atccctttca taatgggcat caatatcata ttaatcaatc
     9601 taaaaaactt acaaatgctg acgttactat tgcaataatg agtggtaact ttgtcatgcg
     9661 tggcgaacca gcaatctata ataagtttac tcgtgcaaaa atggcattat caacagctga
     9721 tttagttatc gaactaccag caactgccag tttatcatct ggcgatcatt ttgccgaact
     9781 agcagttaaa gtctcagatt atatgagtgt cgatacaatt gcatttggta gtgaaaataa
     9841 taatatcaaa acattaaagc aattagctca cagcattaat gaaattgaac aatccgaatc
     9901 cttttcacaa aaagtaaaag aaggtaaaag ctaccctcgt atcattagcg aattattaga
     9961 gcatcatgaa gcactagcaa gtcctaacaa catacttggt attagttatc tgaaagcaat
    10021 tgctaaacat gctaaaaaca tcaatgcaat ttctattaag cgagaaaatg ctcaacatca
    10081 tgattcatta attcaacatc ataagtttgc aagtggtaca tctattagaa catcaatcat
    10141 tagtcaagat gatcattggc atcatgtggt acctaaagat attcaacacc tgtacgtaac
    10201 acctcatata acattaaatc aaatattccc atatttgaaa tatcaaatca tagctatgac
    10261 tacagagtca ttaaagaata tttatactgt tactgaaggt tttgaaaatc gtttaaaatc
    10321 ttacattaat gaagcaactg attttcatca ttttgtaaaa tcactaaaaa caaagcgtta
    10381 tacatatacg catatccaaa gactgttaat gaatgtatta ttaaacatta aacctacaga
    10441 tgttacgaga aacattcacg cagttaaagt attagcaatg aacgaccgtg gcagacaata
    10501 tttaaagcat ttaaaaacag tgttcccaga aagacaatat ataaccaata ttaacaaaag
    10561 caatgcacat tacttcacaa atgaaatcaa aacaacacac atatacaatg caataagcgg
    10621 gcaacaacaa acagatttca acacgcctgt catacagcaa tatcgttaac aaatgaaaac
    10681 agtattttag gattgtaaac catgataaac ctaaaatact gttattttta ttacttaaat
    10741 ttcttcttca atgccttttc aacataaggt ggaacgaatt cagaaatatc tgctcgataa
    10801 gctgcaactt ctttaacaat acttgaactt ataaatgaat aattagtact agacatcata
    10861 tataacgttt caatttcatt gttcaacttt ttattcattg aagttaagcg taattcatat
    10921 tcaaaatcac tgaccgctct taaaccacgt atgattgttt tagctcctac ttgttcacaa
    10981 taatcgacta gtaaaccact aaattgatga accttgacat taggtaaatg tttaacagat
    11041 tgttcaatta aatccatacg ctcttctaaa ctaaacgtac cttctttttt actattttta
    11101 agaacacaga catgaatttc atcaaatcta tctgtacttc tctcaataat gtctaaatga
    11161 ccataagtaa tggggtcaaa actacccgga atgaccgcta ttgtatgttc cattctattc
    11221 tcccttttct aataacaatg tgtctgtcaa cccataatgg taacgtttaa tcatattaaa
    11281 cggttgatag tctatttctt catgattgct aaattcacaa acgatgatac cattttcttt
    11341 caataaatta aactctgaaa ttagttttaa agctttatca atgagacctt tattataagg
    11401 tgggtctaag aaaatgacat caaattgaat atcacgtttt gacaatgctt ttaaagctct
    11461 atctgcatta tttttataaa cttcagattg tgcctctaaa tccaaattcg caagatttga
    11521 tttaataact tttacagctt taaaattttg atcaacaaag ataaccttat ctatacctcg
    11581 agagagtgct tctattccaa gcgccccgct tcctgcaaat aaatctaaac ctatacctga
    11641 cacatcatat aaactattaa agataccttc tttaacttta tccatagttg gtctcgtatt
    11701 acggccttcc atactttcta aagctttact tttatgttta cctgcaatga cgcgcatgtt
    11761 gttcacactt ccaattcatt tagttattta atataattta ttgagaaaaa ggagaatgat
    11821 aaaccaatga aacaaacatt tattacactt ggtgaaggtc taacagattt gttcgaattc
    11881 atgacgatga ttgaatataa ccatcaacgt attgataaaa ttatctattt tcattcacca
    11941 caagctgaaa ataaaaagtc atctgtagca atcattatga accctacaac tggcaatcat
    12001 ttccaagcat tttatatcat gattaacgct attaaatatc catatccaga ttcaaataaa
    12061 aagtttcaaa tgattaatga ttgcgctgaa aaattcgaca taccaatttt aggtatcgat
    12121 gtacagcctc ctcaagcatt tcatgattta tcgttatatt
//
LOCUS       DAKSQE010000080        12099 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.080, whole genome shotgun sequence.
ACCESSION   DAKSQE010000080 DAKSQE010000000
VERSION     DAKSQE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12099
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.080"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..97
                     /locus_tag="O0V33_001912"
     CDS             <1..97
                     /locus_tag="O0V33_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MSA protein"
                     /protein_id="HCX8976536.1"
                     /translation="FGFDEILFFTLYLLLILIVLYYLKKQLKNKT"
     gene            complement(333..1598)
                     /gene="lysA"
                     /locus_tag="O0V33_001913"
     CDS             complement(333..1598)
                     /gene="lysA"
                     /locus_tag="O0V33_001913"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCX8976537.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCLQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAIKAINKVQQSKYLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQDIQVELLNLGGGFGIKYVEGDESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPEINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEEADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDTK"
     gene            complement(1588..2673)
                     /locus_tag="O0V33_001914"
     CDS             complement(1588..2673)
                     /locus_tag="O0V33_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCX8976538.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHEIVQSLTVKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            complement(2678..3829)
                     /locus_tag="O0V33_001915"
     CDS             complement(2678..3829)
                     /locus_tag="O0V33_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCX8976539.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTTLM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     FNVDCEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSYLNFDEKVLINVVNFYENLLNNYKEV"
     gene            complement(3972..4691)
                     /gene="dapD"
                     /locus_tag="O0V33_001916"
     CDS             complement(3972..4691)
                     /gene="dapD"
                     /locus_tag="O0V33_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCX8976540.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFGDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDTKKEIVAALRKLND"
     gene            complement(4718..5440)
                     /locus_tag="O0V33_001917"
     CDS             complement(4718..5440)
                     /locus_tag="O0V33_001917"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCX8976541.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKDADVAIDFSNPNLLFPLLDEALHLPLVVATTGEKEKLLNKLDELSKNMPVFF
                     SANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            complement(5437..6324)
                     /locus_tag="O0V33_001918"
     CDS             complement(5437..6324)
                     /locus_tag="O0V33_001918"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCX8976542.1"
                     /translation="MTHLFEGVGVALTTPFTNNKVNLEALKAHVNFLLENNAQAIIVN
                     GTTAESPTLTTDEKELILKTVIDLVDKRVPVIAGTGTNDTEKSIQASIQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAIADAVKLPVVLYNVPSRTNMTIEPETVEILSQHPYIV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLGFGNYELRLPLVSLED
                     TDTKVLREAYDTFKAGENE"
     gene            complement(6326..7315)
                     /locus_tag="O0V33_001919"
     CDS             complement(6326..7315)
                     /locus_tag="O0V33_001919"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCX8976543.1"
                     /translation="MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGKEV
                     EFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKGGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLEKEATAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            complement(7379..8584)
                     /locus_tag="O0V33_001920"
     CDS             complement(7379..8584)
                     /locus_tag="O0V33_001920"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCX8976544.1"
                     /translation="MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKTPKQQELALLLTTGEQQTVSYLSMVLNDIGVNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINDHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIMSNEEILERKAVTGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILKTLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
     gene            complement(9441..11042)
                     /locus_tag="O0V33_001921"
     CDS             complement(9441..11042)
                     /locus_tag="O0V33_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCX8976545.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNVSFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSFKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
     gene            11189..12091
                     /gene="cvfB"
                     /locus_tag="O0V33_001922"
     CDS             11189..12091
                     /gene="cvfB"
                     /locus_tag="O0V33_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HCX8976546.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDHLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKH
                     KIINIETGKITLTKKGWSRIESKE"
BASE COUNT         3775 a         2232 c         1887 g         4205 t
ORIGIN      
        1 ttttggtttt gatgaaattt tattttttac actttatttg ttattaatcc ttatcgtttt
       61 atattatttg aaaaagcaat taaagaataa aacctaattt catgatgctt gtttaaagtg
      121 tggtatgtat atgatgcttt aaattagttg aatagagcta actaaattaa aggattaacg
      181 acaaaaaata aacactttct aactaaaaaa gtgctgttct tcctttttta gagtatatgt
      241 ccaagcatgt tatctacagc catctataat gcttaaaaat tccccgagtc acagtataat
      301 tttctgttct tcggggatta ctttatttat ttttattttg tatcattaat aataagttgt
      361 cttaacgatt gtcgcttaat aacttcacgt gctttgccat cttttaaaaa aaacacagaa
      421 ggcttttgca tttgattgta attggatgcc atagagtaat gatatgcacc agttgataat
      481 atagcaagat agtctccacg tttgactgat gaaggtaatt tagcgtcttt aataatgata
      541 tcaccagact cacataattt tccagctata gttacactgt catctgcttc ttcatttcta
      601 ttaacaagca atgcttgata ctttgcgtca taaagtgcag ttctgatatg atcactcata
      661 ccgccatcga ttgaaacata tttatttatc tctggaattt ctttaatggt tccaacttca
      721 tataaagtaa cgccagcttc acctacaata gatcgtcctg gttcaatacc tatttctggt
      781 gcatctatac ctagaacttt aatttcggat tttattgcgt ctgtaatatc tttaatacca
      841 ctttcgatag ggaaactttc atcaccttca acatatttaa taccaaagcc accaccaagg
      901 tttaataatt caacttgaat gtcttgctct ttaagccaac gtaaaacaat tttagcagtt
      961 tcaataaatg cttctgtacc ttcaatctgt gaaccaatat gacaatgtac accttttaat
     1021 ttaagatact tagattgttg cactttgtta attgctttta tagctaagcc atattgaatt
     1081 gataatccaa acttactatc ttcttgacca gtttgaataa attcgtgtgt atgtgcttca
     1141 acacctggat taactcgtaa tacaacttga accgtatcat tagcatagcg gtctattaat
     1201 tcaatttctt ctaatgaatc tataacaaaa taaccgatgt tattttctaa agcataccta
     1261 atttcatgtt tcgttttatt gttaccatgg aaatggatgc gacttggttc aaatcccgct
     1321 tctaatgccg tatataattc accttcagaa acaacatcta actgtaaatc ttcctcagcc
     1381 accagtttga ccatttgaag acaagtaaat gctttagagg cgtatgaaat attgtatttt
     1441 aatccactat ctttaaatgc gcgatggtaa cggcgcatct gttctctaat ttgtagttca
     1501 tcataaacaa tggtaggtgt accaaagctt tgcgcaatcg tttttaaact aataccatcc
     1561 attgttaatt cgccattttg attatattta acagtcattt gaaatgtact ccttttttaa
     1621 agaatcatga ttcatagcac ttaattgttc agaatttgca ccaacacctt taaaggtata
     1681 ttcatcgatg cggatatcat tattataaag aataacttca tcttgtgcat gtacattgcc
     1741 atctacttca acaaacatat ggctcatcat taatgcacgt atcgggtagc gtttaccatt
     1801 gataagtgct tcatgtttag cacgagttcg taaaattcca tcgccataac cgatatcaac
     1861 tacagctaat tttgtattgt ttttagtcac ttcaaaggca aagctataac cgcaataatc
     1921 accagcttgt acttcgcgca cttgaataac atgtgctttt acagtcaatg actgaactat
     1981 ttcatgttga ttcagtgaac tatatggtct tgaaccgtat aacgcaatac ctacacgcgc
     2041 atgtgtatgg tggggtagta atatttgtcc ttcccgataa aaactcgcac tattttgagc
     2101 atggattagg tcgaactgat aaccttcaga taaaagtgct tcaacaattt ccatccattg
     2161 tgaacgttca acattataat ctgacacatc gaattcatca gcatatccaa aatgggtcca
     2221 taaaccacta ataatcattt ttgcattttg attatgatgg tgatctttca atacttcttt
     2281 aatttcgttt aaatctttaa atccagaccg atgtaataaa ttttcaaatt ctaagtgaac
     2341 atgaatacct gctaaatcat ttttatggtt atagtaatat gtcaacgacg gcaaagtcat
     2401 gtgtatttga tgttcacgga ctaaatcaaa ctcgtaaact gcattcatta aaaagattgt
     2461 tgcatctgga gcaagttgtc taatttgaat tgcttctcgt agtgatgttg tgctaaatgt
     2521 atctatacct gcatggataa actgagttac agcaaattct aggtcatagt gatatgcatt
     2581 atttttaaca actgccatta atggctgatt gtttttgact gtgattgcat tttgtaaaaa
     2641 tattttctta tttacagacc atgttgctgt caatgtatta cacctctttg taattattta
     2701 ataaattttc gtaaaaatta accacgttta ttaacacttt ttcatcaaaa tttaaatatg
     2761 atgtgtgcaa accagttaca aaacctttat cttcatttcg tgttcctata aaaacaaagt
     2821 aagctggagc tagttgttga ccataaaaac taaaatcttc cccaaataag aatggcgttg
     2881 gtttgtcata gacatttaaa tcagctttta ttaaggcgtc ctctatttga gtacgtaatt
     2941 tcggactatt gattgtaggg ggataacctt ctgcaaattt aacttcacag tctacattaa
     3001 acagaagctt gacactttct gctatcttgt gcatttgatt tttaacgatt gttaaatcat
     3061 caatatcata tgtacgaatt gtaccttcta aataaccatt acttggtaca gtgttaatcg
     3121 cttcaccagc tttaaaatga ccaatatgaa caatatttcg ttttaaaccg ttaaggtgaa
     3181 attgttgaat ttgtgatatt tgagttaata catgttgtaa tgcttcacca caagaatgac
     3241 cttgttcttt atcagcaaca tgacttgaca ggcctgttaa gaaaaagcga tactctgttg
     3301 cgctggccgt aatttcttca tctcttatca ctgcaatgcc ttcatcagca aatgggttaa
     3361 catgaatacc aaataccgct tcaattggat acttatcaaa ggcaccggct tttattaatc
     3421 gatttgcacc gccaccagtt tcttctgcag gttggaaaat gaaaacgaca ttttgcggta
     3481 attgccccgc atcttgcatg tctttgcaac gttgtacaaa aagcattaat gtagttgtat
     3541 gaccatcatg tccacaagca tgcatcacat gatcagattg actgcgataa ggtacatcat
     3601 tttcctccaa aataggtaat gcatcaatat cagctctata cgctatcgta tgtgagccat
     3661 taccttctaa gtatgcaatg acgccagttt ccaatgggca atcgtattta atatttaaac
     3721 tatctaaaaa cgctttaata taagcagttg tttcaaattc atgtaagctt aattcaggat
     3781 gttgatgtaa atgacggcga tgtttcgtaa caaattctaa ttcattcata attatcaatc
     3841 ctttgtgtta aattactata taaatagtgt aacgtatttc gaaatttgtg atcataagtt
     3901 tatttaatga taaacaataa ggttgagaca taatcgtatc tcaaccttga aattattata
     3961 cgttgacgtc actagtcatt cagttttctt aatgctgcta caatctcttt tttagtatct
     4021 tgtacttcag aagcttgctt aatcactttc gcaggtgtac cagcaacaac tgcaccagct
     4081 ggtacatctt gagtcacaat cgcgccagct gcaacaatag cacctttacc aacacgtaca
     4141 ccttctaaaa taactgcatt tgcgccgatt aatacatcat cctcgattat aactggtgaa
     4201 gcactagggg gttcaatcac acctgctaat actgcgccag cccctacatg tacattttta
     4261 ccagttgtag cacgaccacc gagagtagca ttcatatcaa tcatcgtacc ttcgccaacg
     4321 actgcgccaa tattaattgt tgcacccatc ataacgacag caccatcttc aataatagct
     4381 tgttctctaa taaatgcacc tggttcaata cgtgcattag tatttgttaa gtcttttaat
     4441 ggaatggcag aattgcgacg atccatttca atttctatat ctccgaattg actaccatat
     4501 gcttcgtaaa aaggtttcca atcatctgct tcacaaaaga ttactttaga ttgttctgaa
     4561 ccaaatactt taaaactttc tggatatgtg atgccttcaa aattgccatt taaatatact
     4621 ttaattggtg tagatttttt agcatcactt atatattgaa taatttcttc agctgttaaa
     4681 tgttgtacca taaaataatc gatctccttt aatatgttta taggttgtca aacgtataaa
     4741 agccgtttgg tttattaact aagcgttctg ctgcttgtat tgcaccattc gcaaaaatat
     4801 cttttgattg tgcacgatgc gtgatttgaa tcgtttcatc agtgccggca aatagaactt
     4861 catgttcacc gacaatcgta cctccacgaa tagaatgaat accaatatct tgtggctggc
     4921 gtttttcatt taattcatgt ctatcataaa caggagttac attttctttc aaagatacaa
     4981 tcacgtcata taatttttct aacgtaccgc ttggtgcatc tactttttta ttatgatgtg
     5041 cctcagtcaa ttcgatgtcg aaatcatcaa gtaggggaac agcagctgct aaaattttag
     5101 tcagtgcatg aacgccataa ctcatgttcg cgctgaaaaa cacaggcata tttttactca
     5161 attcatctaa cttattaagt agtttttctt tctcgccagt tgttgccacg actaatggca
     5221 aatgaagcgc ttcatctaat aaagggaaaa gcagatttgg atttgaaaaa tctattgcaa
     5281 catctgcatc tttaacatca gcaatatgtt ggtattgttg atatggcgtt gttgctttcg
     5341 gtgtattttc aatgacccca acgatttcat gtcctttttc ttctgctaat ctagcaacgc
     5401 gctgattcat tgcaccatag ccaattagta atattttcac tcattttcac ccgctttaaa
     5461 tgtgtcatat gcttcacgaa gcactttagt atctgtatct tctaggctaa ccaatggtaa
     5521 acgtaattca tagtttccaa atcctaaata acttgttaaa gctttaatag gaattgggtt
     5581 aatatcaact gatagggctg ataacagtgt gccgattggt ttaaattgat cttgaatatc
     5641 taatccactt tgttgagcat cgtataacgc ttgaaattct ttaggaatga cattggcaat
     5701 aacagagata accccttgac cgccacgttg atagtattcg acgacgttgt catcattgcc
     5761 actatataat gcaaatgaat ttgtatcaat tcgctttttc acttcttcta aatactcaaa
     5821 atcattcgta gcatctttta aagcaactat ataaggatgt tgacttaata tttctacagt
     5881 ttctggttca attgtcatgt tcgttcttga aggaacattg tacagcacga ctggtaattt
     5941 cacagcatct gcaatcgctt caaaatgttt tactaaacca cgttgattcg ttttgttgta
     6001 gtagggtgta attaacataa ttgcatcagc ccctaaggct ttagcttgga ttgaagcttg
     6061 gattgacttt tcagtatcat tagttccagt tccagctatg acaggaacac gtttatctac
     6121 aagatcaata actgttttta gaatgagttc tttttcatct gttgttaaag tagggctctc
     6181 agcagtagtt ccattaacga tgattgcttg ggcattattt tctagtaaaa aattaacgtg
     6241 tgctttcaaa gcttcaagat taactttgtt atttgtaaaa ggggttgtaa gtgcaacgcc
     6301 aacaccctca aataaatgtg tcattttaat tcgctccttt taaacgcata acttgttcca
     6361 atacttgtac agcatttaat gcagcacctt ttaataaatt gtctgatgta caccatacat
     6421 ggaaagtatt ttctaatgaa tcatctctac gtatacggcc aacaaatact tcatctttat
     6481 tagtagaatt gattgccatt ggatattcat tgttctctgg attgtctact aaaacaacgc
     6541 gatcatcttg atcaaataat gctttaatat cttctgcagt cgcttctttt tcaagtgtta
     6601 catcaatttc aacactatga ctatcttgaa caggaacacg tgcacatgtt gctgttactt
     6661 ttaagtctgg cgcatttaaa atttttctcg tctcatcaat cattttttgt tcttcttttg
     6721 tatatccgtt ttctaaaaac acatcaatat gcggtaacac attattataa attggatgtg
     6781 gatatgcttc tggtgcttta ccatttacac cttcagctaa atctttctta cctttcatcc
     6841 ctgaacctga tacagcttga tatgttgtat atgccactcg ttttaaacca taagcatctt
     6901 gcaatacttt taatggcaca acagactgaa tcgtagagca gtttggattg gcaatgatac
     6961 ctcttgtaaa agtaggttca ttgacttccg gaacgattaa atcaatatct tctgccatac
     7021 gccattgact tgaattgtct ataacgattg caccaccttt ttcaaagagt ggggcaaagt
     7081 gttcgcttgt accgccacca gcactcatta atacataatc gaaatgttca cttgcacgag
     7141 catcagttaa ttcttgaact gtatatgttt ttccttgaaa ttcaacttct ttccctgcag
     7201 aacgtgctga tgaaaataat actaattcat cgaaaggaat atttttacga tttaatgtct
     7261 ccaacatttt tgtacctact aatcctgttg cacccacaac tgctaatttt gtcataactt
     7321 gtcactccat tttatgataa tttccaattt ttagaatatt ttaacaatca ttttaccatt
     7381 aaatgttaaa tgcgtcatat agtttttcca ccgcttgttg cccattaaaa tcatcaatga
     7441 cgtatgaaat acttatttca gatgttgttg tttggtagaa aggtatatta ttttcaatta
     7501 atgtcaaaaa tgcttttgat gccacacctg acatatcacg catgcctgag ccaattaatg
     7561 aaattttgac ataatgctca ttgattttat aagctaatgc ttcatattga ttctttaatg
     7621 ttttaagaat catagaaatt tgatgaaaat cactatcttt aatcgtgaag gatagttgta
     7681 gtccatccaa gttgacgatt tgtgaaatca tatcaacatt tacagcacct tcttcaagtt
     7741 ccgtaaatag ttgggtaagt agctgattgt caggtagggg ataactaatt gttacatgca
     7801 tcatatgttt atccaaagcc acaccagtaa ctgcttttct ctctaatatt tcttcatttg
     7861 acataatcca tgttcctttc acgttcgata aagtttttcc taaatataaa gggatattat
     7921 agtttttagc taattcaaca cttcttgttt caagtacacc agcacctaaa gcgctcattt
     7981 ccatcatttc ttcatatgag acgatgtcta gtcgtttagc ttttggtaaa agtcttgggt
     8041 cagtggcata cacaccatca acgtcggtat aaatttcaca aggtatttga ttactaacag
     8101 caagtgccac agcggtcgta tcagaaccac ctctgcctaa agttgttaat tcctgatggt
     8161 cattgatgcc ttgaaatcca gcaactacta aaatatcgtt ttcttgaaag gcttgttcaa
     8221 atgtttgagg attaatttga gcaattttac tttttaaatg atggccaatg gttttaatac
     8281 ccgcttgata gccagtcatt gcttttgcat tcacaccgat atcatttaat accattgata
     8341 aataagatac agtttgttgc tctccggttg tcaataataa tgccagttct tgttgttttg
     8401 gtgttttagt caatgtcgat acattcgtca ttaattgatc tgttgtgtta cccatagcac
     8461 ttacaacgac aattaattgt tcatcttgat tgacccgctc ctttaacatt tcagcgatcc
     8521 tttttatttt tgtaaaatca ctgacggatg atccgccaaa tttcaacacg cttcttgtta
     8581 ccatataatc ctcctaattg ttaggaaggg cacacaataa taaaaaaaga acaagttcgg
     8641 aatgcgaact tgctcttaat ctatatacaa gtgatgcact ccattatttt taaataatga
     8701 caaactctca gctcttaacc aaaaagtcca acaaattata actgctatta taatcgcttc
     8761 ggcatcgcac cctttcaaat ttagctgtta gcagacagta atctaaactt tactcatgat
     8821 tgatgcgcct catcaaaata ttattaaatt ggtatgtgtt cattatacat atgacaaata
     8881 tgaatgtaaa ccgataattt agattttttg gaataacctg aaaattcaag ttatagcgtt
     8941 gcttatatta taaaaggtgg tgagaatgaa acttttttga aaaataaaat ccgaaatact
     9001 tatagcgtac tcaatatgca cattaaataa atgtactctt ttaatgcgtt gataactgta
     9061 tttgtaattt agagaagggg tgttcactgt gctgatgtgc taaaaaataa aaaagacacc
     9121 tcgatgctat aaatattagc atcgagacgt ccagttaatg tctattaaat tgaatatagt
     9181 ctcggacatg aatcaatgcc ctaggccctg caatgttata ttgacagtag ttgactgaat
     9241 gaaaatgact ttgtacctag cttttttcaa tacttgtcag cgtctcacat agagaaattg
     9301 gattcctaat ttctacaaac aatacgagtt ggggaataag tcccaatata gaaggtgaca
     9361 gtaatgtcaa cttacaaaaa tgtgcaagtt ggccgggcct caacacagag attttcgaaa
     9421 agaaattcta caaattatat ttattttaaa acgccgattt cttgcaagta ttcttcatat
     9481 ggaatttctt ttgaaacgcc gccttgttta tttaaatcga taacacggtt tgcaatcgtg
     9541 ttgataaatt caaagtcata agaagtaaag atgatagaac ctttaaatga tttaaggcca
     9601 tcattgacag cagtaatact ttctaagtct aagtggttag taggttcgtc aagtaaaagt
     9661 acattcgcac ttgataacat cattttactt agcatacaac gtactttttc tccacctgaa
     9721 agcacactag cttttttctt aacttcttca ccactaaata acatacgacc taagaaacca
     9781 cgtaaaaatg tttctgtttg ttcatcttca ggagcatatt gtcttaacca atcaacgaga
     9841 ttcatattta caccctcaaa gaactctgag ttatctttag ggaagtaact taacgatgta
     9901 gtaacacccc atttaaatga accttcgtct ggttccattt cgccagctaa tattttaagt
     9961 aatgttgttt ttgcaatttc actatctcca attaaaatcg ctttatcatt tggattcatt
    10021 gtgaaggata cattatctaa tactttttcg ccgtcaattg ttttagaaag attttgaacg
    10081 attaataagt cgttaccaat ctcacgctca ggcgtgaatt taacgaaagg atatcttctt
    10141 gatgatggtt gaatatcatc taattcaatt ttctcaagtt gttttttacg acttgttgct
    10201 tgtttagatt tagaagcgtt agctgagaaa cgagcaataa agtcctgtaa ctctttcatt
    10261 ttttcttctt ttttcttgtt ttgttcttga gccatctttt gagctaactg actagattga
    10321 taccaaaaat catagttacc aacataaact ttaattttac caaagtctaa atcagcgata
    10381 tgagtacata cattatttaa gaaatgacgg tcatgtgata ctacgataac agtattatca
    10441 aagttaatta agaaatcttc taaccaactg attgctggaa tatcgagacc gttagtaggc
    10501 tcatccagta atagtacgtc tggttcaccg aataaacttt gcgctaataa tactttaatt
    10561 ttttggttgt tttctaattc agccattttt ttatcgtgta aagttggatc gatacctaaa
    10621 ccggataaaa ggttagctgc atcagcttca gcattccaac cattcatttc tgcaaattca
    10681 ccttcaagtt cagcagcacg gataccatct tcatcactga aatctggctt catatagatt
    10741 tcatcttttt ctttcataac ctcataaaga cgttcgtgac ctttaattac aacatcaagc
    10801 acgcgttcat cttcataagc atagtggtcc tgttttaaaa cagctagacg ttcattttta
    10861 cctaatgaaa catgtcctgt ttgagaatct aattcaccag ataatatttt taagaatgtt
    10921 gatttacctg caccattcgc accaattaat ccataacaat taccttctgt aaatttaata
    10981 tttacatctt caaatagttt acgatctcca aaacgtaaac tcacatcagt tacttgtaac
    11041 atgcattttc tccttttttt cattcgatat tctaacggaa gaattatatc atattatcgt
    11101 cacagtttcg acctcatata agttgtaatg atagaatgac tcacacatgt tataataata
    11161 aagaatacaa gaatcgaagg agaataacat ggcattagac aaagatatag taggttctat
    11221 agaattcctt gaagtagtag ggttacaagg ctcaacttac cttttaaaag gaccaaacgg
    11281 tgaaaacgta aagttaaacc aatcagaaat gaacgatgat gatgaattag aagtaggtga
    11341 agaatatagt ttcttcattt atccaaaccg ttcaggtgaa ttatttgcaa ctcaaaatat
    11401 gcctgatatt acgaaagata aatatgattt tgctaaagta cttaaaacgg atcgcgatgg
    11461 ggcacgtata gatgttggat taccccgtga agtgttagta ccatgggaag atttaccaaa
    11521 agtgaaatca ctatggccac aacctggaga tcatttgcta gttacattac gaattgaccg
    11581 tgagaatcat atgtatggac gtttagcgag tgaatctgtt gtagaaaata tgtttacacc
    11641 tgtacacgac gataatttaa aaaacgaagt cattgaagcc aaaccatacc gcgtattacg
    11701 aattggtagc ttcttattaa gcgaatcagg ttacaaaatt tttgtacatg aatcagaacg
    11761 taaagctgaa ccgagactag gtgaatctgt tcaagttaga attatcggtc ataatgataa
    11821 aggtgagtta aatggttcat ttttaccact tgcacatgaa cgtttagacg atgacggtca
    11881 agtcatcttt gatttactag ttgaatatga tggtgaatta ccattctggg acaaatcaag
    11941 ccctgaagcg attaaagaag tattcaatat gagtaaaggt tcattcaaac gtgcaatcgg
    12001 tcacttatat aaacataaga tcattaatat agaaactggt aaaatcactt taactaaaaa
    12061 aggttggagt cgaattgagt caaaagaata atcattttt
//
LOCUS       DAKSQE010000081        12027 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.081, whole genome shotgun sequence.
ACCESSION   DAKSQE010000081 DAKSQE010000000
VERSION     DAKSQE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12027
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.081"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            178..1509
                     /locus_tag="O0V33_001923"
     CDS             178..1509
                     /locus_tag="O0V33_001923"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCX8976547.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
     gene            1835..2410
                     /locus_tag="O0V33_001924"
     CDS             1835..2410
                     /locus_tag="O0V33_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCX8976548.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRLVDTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLIVFWIVGKKLEQHFMGSKKE"
     gene            2415..2939
                     /gene="lepB"
                     /locus_tag="O0V33_001925"
     CDS             2415..2939
                     /gene="lepB"
                     /locus_tag="O0V33_001925"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCX8976549.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKKLDGDIIPPNNFVVLNDHDNNQHDSRQFGLIDKKDIIGNISL
                     RYYPFSKWTIQFKS"
     gene            2955..3530
                     /gene="lepB"
                     /locus_tag="O0V33_001926"
     CDS             2955..3530
                     /gene="lepB"
                     /locus_tag="O0V33_001926"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCX8976550.1"
                     /translation="MKKELLEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIIGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKYNFNPENTKN"
     gene            3690..7166
                     /gene="addB"
                     /locus_tag="O0V33_001927"
     CDS             3690..7166
                     /gene="addB"
                     /locus_tag="O0V33_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HCX8976551.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLESFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNDFITGEDALQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEISNELNISIERQYFNQLYRFNN
                     QDLKHLEQEFDALQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQINPMLR
                     LLKTDVLTTSYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAATVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYVAMTRAKGNVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLDDEIVADSWLDAYQVIRDSDHLSQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWADIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGEQ"
     gene            7167..10820
                     /gene="addA"
                     /locus_tag="O0V33_001928"
     CDS             7167..10820
                     /gene="addA"
                     /locus_tag="O0V33_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCX8976552.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYETGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPTNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDGVDKHLAVIEDERRLMGRVLEGGFIDISYLTGH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFHEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTLDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVRYDEAAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVFDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYKAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SASIPDDLKFEKDIAQIEDSSRPNVNISIIYFEDVSTETILDNDEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYQYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADT
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVK
                     GYLYFFKFGTLQL"
     gene            10986..11888
                     /locus_tag="O0V33_001929"
     CDS             10986..11888
                     /locus_tag="O0V33_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCX8976553.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVERLYVFTKAASSLTGDNAKIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
BASE COUNT         4385 a         1658 c         2196 g         3788 t
ORIGIN      
        1 gcaaattgta taaaaaatgt ttgagtgttt gacgccggtt tgaaatgttt gttgaaaaat
       61 aggtgtgaaa cgtcacataa atatcaattt tattgattaa gtacaaactt gtgaattgaa
      121 tcgcaaacgt aaacttagta aaattaagat attaaatgaa acaggaggct tttatagatg
      181 actcatattc aattagattt tagtaaaacg ttagaatttt tcggtgaaca cgaattaaaa
      241 caacaacaag aaattgttaa atcaattcac aaaacaattc atgaaggtac tggtgcaggt
      301 agtgacttct taggctgggt tgatttacca gttgattacg acaaagaaga attttcaaga
      361 attgttgaag catcaaaacg cattaaagaa aattctgatg ttttagtagt catcggtatt
      421 ggtggttctt acttaggtgc acgtgcagca atcgaaatgt taacgtcatc atttagaaac
      481 agcaatgaat accctgaaat tgtatttgtt ggtaatcact tatcatcaac atacactaaa
      541 gagttagttg attatttagc agacaaagat ttctctgtaa acgttatttc taaatctggt
      601 acaactacag aaccagcagt tgcatttaga ttgttcaaac aattagttga agaaagatac
      661 ggtaaagaag aagcacaaaa acgtatattt gcaacaacgg ataaagaaaa aggtgcttta
      721 aaacagttgg ctacaaacga aggttatgaa acgtttatcg tacctgatga tgtaggtgga
      781 agatattctg ttttaacagc agtaggatta ttaccaattg caacagctgg aattaacatc
      841 gaagctatga tgattggtgc tgcaaaagca cgtgaagaat tatcttcaga taaattagaa
      901 gaaaacattg cataccaata tgcgacaatt cgaaacattt tatatgcaaa aggttataca
      961 acagaaatgt tgattaacta tgaaccatct atgcaatact ttaatgaatg gtggaaacaa
     1021 ttatttggtg aatcagaagg taaagacttc aaaggtattt atccttcaag tgccaactac
     1081 acaactgatt tacattcttt aggtcaatat gtacaagaag gccgtcgttt cttattcgaa
     1141 acagttgtaa aagtaaatca tcctaaatat gatattacta ttgaaaaaga tagtgatgat
     1201 ctagacggat taaattattt agctggtaaa acaatcgatg aagttaacac aaaagcattc
     1261 gaaggtacat tattagcgca tactgatggt ggtgttccta acatggtagt gaacattcca
     1321 caattagatg aagaaacttt cggttatgtc gtatacttct tcgaacttgc ttgtgcaatg
     1381 agtggatacc aattaggcgt aaatccattt aaccaacctg gtgtagaagc atataaacaa
     1441 aacatgttcg cattattagg taaacctggt tttgaagact tgaaaaaaga attagaagaa
     1501 cgtttataaa atacattact tcaatgatta gtgaagtttg aaaagataga actagacgtt
     1561 aactatttaa agcatatttc cgaggttgtc attacaaatg taaaaatgta atgacaacct
     1621 cgtttttatt ttatatgcaa gatataggtt actagctaat gtgacaagat gttaagagaa
     1681 aattaaagag aaaataatat ctgccataca ataaaattgt tatactacta gagactgatt
     1741 tattagcatg attacatgtt aatgtttctt tacttagtaa ttaactttat aatgtaagaa
     1801 taattatctt cagccaaaga aagggattga tgatttgtcg tttcatcaag tagaagaatg
     1861 gtttgagata tttcgacagt ttggttattt acctggattc atattgttat atataagagc
     1921 gattattcca gtatttccat tagcactcta tattttaatt aatattcaag cctatggacc
     1981 tattttaggt atattgatta gttggcttgg attaatttct ggaacattta cagtctattt
     2041 gatctgtaaa cgattggtgg atactgaaag gatgcagcga attaaacaac gtactgctgt
     2101 tcaacgcttg attagtttta ttgatcgcca aggattaatc ccattattta ttttactttg
     2161 ttttcctttt acgccaaata cattaataaa ttttgtagcg agtctatctc atattagacc
     2221 taaatattat ttcattgttt tggcatcatc aaagttagtt tcaacaatta ttttaggtta
     2281 tttaggaaag gaaattacta caattttaac gcatccttta agagggatat taatgttagt
     2341 tgtgttgatt gtattttgga ttgttggaaa aaagttagaa cagcatttta tggggtcgaa
     2401 aaaggagtga catcgtgaaa aaagttgtaa aatatttgat ttcattgata cttgctatta
     2461 tcattgtact gttcgtacaa acttttgtaa tagttggtca tgtcattccg aataatgata
     2521 tgtcaccaac ccttaacaaa ggggatcgtg ttattgtaaa taaaattaaa gttacattta
     2581 atcaattgaa taatggtgat atcattacat ataggcgtgg taacgagata tatactagtc
     2641 gaattattgc caagcctggt caatcaatgg cgtttcgtca gggacaatta taccgtgatg
     2701 accgaccggt tgacgcatct tatgccaaga acagaaaaat taaagatttt agtttgcgca
     2761 attttaaaaa attagatgga gatattatac cgcctaacaa ttttgttgtg ctaaatgatc
     2821 atgataacaa tcagcatgat tctagacaat ttggtttaat tgataaaaag gatattattg
     2881 gtaatataag tttgagatat tatccttttt caaaatggac gattcagttc aaatcttaaa
     2941 aagaggtgtc aaaattgaaa aaagaattat tggaatggat tatttcaatt gcagtcgctt
     3001 ttgtcatttt atttatagta ggtaaattta ttgttacacc atatacaatt aaaggtgaat
     3061 caatggatcc aactttgaaa gatggcgagc gagtagctgt aaacattatt ggatataaaa
     3121 caggtggttt ggaaaaaggt aatgtagttg tcttccatgc aaacaaaaat gatgactatg
     3181 ttaaacgtgt catcggtgtt cctggtgata aagtagaata taaaaatgat acattatatg
     3241 tcaatggcaa aaaacaagat gaaccatatt taaactacaa tttaaaacat aaacaaggtg
     3301 attacattac tgggactttc caagttaaag atttaccgaa tgcgaaccct aaatcaaatg
     3361 taattccaaa aggtaaatat ttagtgcttg gggataatcg tgaagtaagt aaagatagcc
     3421 gtgcgtttgg cctcattgat gaagaccaaa ttgttggtaa agtttcgttt agattctggc
     3481 cattcagcga atttaaatat aatttcaatc ctgaaaatac taaaaattaa tatgaaacaa
     3541 atacaacatc gtttgtcggt tttaatactg ataaacgatg ttttattttg taataggtat
     3601 gataaaagct aagttcgaaa tgaacttata ataaatcaat cacaatcact ttgtgttaaa
     3661 atatgtgtca aaggaagtga gggtttgtca tgacattaca tgcttattta ggtagagcgg
     3721 gaacaggtaa gtctacgaaa atgttgaccg aaataaaaca aaaaatgaaa gcagatccgc
     3781 ttggagatcc aatcatttta attgcgccaa ctcaaagtac atttcaatta gaacaagcct
     3841 ttgtcaatga tccggaatta aatggtagtt taagaacaga agtgttgcat tttgaacgat
     3901 taagtcatcg tattttccaa gaagttggta gttatagcga acaaaagtta tctaaagctg
     3961 caacggaaat gatgatttat aacattgttc aagaacaaca aaagtattta aaactttatc
     4021 aatcacaagc aaaatattat ggatttagtg aaaaattaac agaacaaatt caagatttta
     4081 aaaaatatgc agtaacgcct gaacatttag aaagctttat tgctgataaa aatatgcaaa
     4141 ctcgaactaa aaataagtta gaggatattg ctttaatata ccgagagttc gaacaacgca
     4201 ttcaaaacga ttttattacg ggtgaggatg cattacaata ttttattgat tgtatgccga
     4261 aatcagagtg gctaaaacgt gctgatatat atattgatgg ttttcacaac ttttcaacga
     4321 ttgagtattt aataatcaaa ggattaatta aatatgcgaa gagtgtcaca attatattga
     4381 cgacagatgg taaccacgat caattcagtt tatttagaaa accatcggaa gtgttacgac
     4441 atattgaaga aatatcaaat gaactcaata tttctattga acgtcaatat ttcaaccaat
     4501 tatatcgctt taataatcaa gatttaaagc atcttgaaca agaatttgat gcacttcaaa
     4561 tcaatcgagt ggcatgtcaa ggtcatatca atattttaga atctgcgact atgagagagg
     4621 aaataaatga aattgcacga cgtatcatcg ttgatattcg tgataaacaa ttacgatatc
     4681 aagatattgc tattttatat cgtgacgagt cttatgctta tttatttgat tccatattac
     4741 cgctttataa tattccttat aacattgata caaagcgttc gatgacacat catccggtca
     4801 tggaaatgat tcgttcattg attgaagtta ttcaatcaaa ttggcaaatt aatccaatgc
     4861 tacgcttatt gaagactgat gtgttaacga catcatatct aaaaagtgca tacttagttg
     4921 atttacttga aaattttgta cttgaacgtg gtatatacgg taaacgttgg ttagatgatg
     4981 agctatttaa tgtcgaacat tttagcaaaa tggggcgtaa agcgcataaa cttaccgaag
     5041 atgaacgtaa cacatttgaa caagtcgtta agttaaagaa agatgtcatt gataaaattt
     5101 tacattttga aaagcaaatg tcacaagcag caactgtaaa agactttgca actgcttttt
     5161 atgaaagtat ggaatatttt gaactgccaa atcaattgat gacagagcga gatgaacttg
     5221 atttaaatgg taatcatgaa aaggcggaag aaattgatca aatatggaat ggcttaattc
     5281 aaatcctcga tgacttagtt ctagtatttg gagatgaacc aatgtcgatg gaacgtttct
     5341 tagaagtatt tgatattggt ttagaacaat tagaatttgt tatgattccg caaacattgg
     5401 accaagttag tattggtacg atggatttgg ctaaagtcga caataagcaa catgtttact
     5461 tagttggaat gaatgatggt acgatgccac aaccagtaac tgcgtcaagc ttgattacag
     5521 atgaagaaaa gaaatacttt gaacagcagg ctaatgtcga gttaagtcca acatcagata
     5581 ttttacagat ggatgaagca tttgtttgtt atgttgctat gactagagct aagggaaatg
     5641 ttacattttc ttatagtcta atgggatcaa gtggtgatga taaggaaatc agcccatttt
     5701 taaatcaaat tcaatcattg ttcaaccaat tggaaattac taacattcct caataccatg
     5761 aagttaaccc attgtcacta atgcaacatg ctaagcaaac caaaattaca ttatttgaag
     5821 cattgcgtgc ttggttagat gatgaaattg tggctgatag ttggttagat gcttatcaag
     5881 taattagaga tagcgatcat ttaagtcaag gtttagatta tttaatgtca gcattaacgt
     5941 ttgacaatga aactgtaaaa ttaggtgaaa cgttgtctaa agatttatat ggtaaggaaa
     6001 tcaatgccag tgtatctcga tttgaaggtt atcaacaatg cccatttaaa cactatgcct
     6061 cacatggtct gaaactaaat gagcgaacga agtatgaact tcaaaacttt gatttaggcg
     6121 atattttcca ttccgtttta aaatatatat ctgaacgtat taatggtgat tttaaacaat
     6181 tagacctgaa aaaaataaga caattaacaa atgaagcatt ggaagaaatt ttacctaaag
     6241 ttcaatttaa tttattaaat tcttcagctt actatcgtta tttatcaaga cgcattggcg
     6301 ctattgtaga aacaacacta agcgcattaa aatatcaagg cacgtattca aagtttatgc
     6361 caaaacattt tgagacaagt tttagaagga aaccaagaac aaatgacgaa ttaattgcac
     6421 aaacattaac gacaactcaa ggtattccaa ttaatattag agggcaaatt gaccgtatcg
     6481 atacgtatac aaagaatgat acaagttttg ttaatatcat tgactataaa tcctctgaag
     6541 gtagtgcgac acttgattta acgaaagtat attatggtat gcaaatgcag atgatgacat
     6601 acatggatat cgttttacaa aataaacaac gccttggatt aacagatatt gtgaaaccag
     6661 gtggtttatt atacttccat gtacatgaac ctagaattaa atttaaatct tgggcagata
     6721 ttgatgaaga taaattagaa caagatttaa ttaaaaagtt taagctgagt ggtttagtga
     6781 atgcagacca aactgttatt gatgcattgg atattcgttt agaacctaaa ttcacttcag
     6841 atattgtacc agttggtttg aataaagatg gttctttgag taaacgaggc agccaagttg
     6901 cagatgaagc aacgatttat aaattcatcc aacataacaa agaaaatttt atagaaaccg
     6961 cttcaaatat tatggatgga catactgaag ttgcaccatt aaagtacaaa caaaaattgc
     7021 catgtgcttt ttgtagttat caatcggtat gtcatgtaga tggcatgatt gatagtaagc
     7081 gatatcgaac tgtagatgaa acaataaatc caattgaagc aattcaaaat attaacatta
     7141 atgatgaatt tgggggtgag caatagatga caattccaga gaaaccacaa ggcgtgattt
     7201 ggactgacgc gcaatggcaa agtatttacg aaactggaca agatgtactt gttgcagccg
     7261 cggcaggttc aggtaaaaca gctgtactag ttgagcgtat tatccaaaag attttacgtg
     7321 atggcattga tgtcgatcga cttttagtcg taacgttcac aaacttaagt gcacgtgaaa
     7381 tgaagcatcg tgtagaccaa cgtattcaag aggcatcgat tgctgatcct gcaaatgcac
     7441 acttgaaaaa ccaacgcatc aaaattcatc aagcacaaat atctacactt catagttttt
     7501 gcttgaaatt aattcaacag cattatgatg tattaaatat tgacccgaac tttagaacaa
     7561 gcagtgaagc tgaaaatatt ttattattag aacaaacgat agatgaggtc atagaacaac
     7621 attacgatat ccttgatcct gcttttattg aattaacaga gcaattgtct tcagatagaa
     7681 gtgatgatca gtttcgaatg attattaaac aattgtattt ctttagcgtt gcaaatccaa
     7741 atcctacaaa ttggttggat caattggtga caccatacga agaagaagca caacaagcgc
     7801 aacttattca actactaaca gacttatcta aagtatttat cacagctgcc tatgatgctt
     7861 taaataaggc gtatgatttg tttagtatga tggatggcgt cgataaacat ttagctgtta
     7921 tagaagatga acgacgttta atggggcgtg ttttagaagg tggctttatt gatatatctt
     7981 atttaactgg tcacgaattt ggtgcgcgtt tgcctaatgt aacagcgaaa attaaagaag
     8041 caaatgaaat gatggtcgat gccttagaag atgctaaact tcagtataaa aaatataaat
     8101 cattaattga taaagtgaaa agtgattact tttcaagaga agctgatgat ttgaaagctg
     8161 atatgcaaca attggcgcca cgagtaaagt accttgcgcg tattgtgaaa gatgttatgt
     8221 cagaattcaa tcgaaaaaag cgtagcaaaa atattctgga tttttctgat tatgaacatt
     8281 ttgcattaca aattttaact aatgaggatg gttcgccttc agaaattgcc gaatcatacc
     8341 gtcaacattt tcatgaaata ttggtcgatg agtatcaaga tacgaaccga gttcaagaga
     8401 aaatactatc ttgcatcaaa acgggtgatg aacataatgg taatttattt atggttggag
     8461 atgttaagca gtccatttat aaatttagac aagctgatcc aagtttattt attgaaaagt
     8521 atcaacggtt tactttagat ggagatggca ctgggcgtcg aattgatttg tcgcaaaact
     8581 tccgttctcg taaagaagta ctgtcaacga caaactatat attcaaacat atgatggatg
     8641 aacaagtcgg tgaagtaaga tatgatgaag cagcacagtt gtattatggt gcaccatatg
     8701 atgaatcgga ccatccagta aacttaaaag tgcttgttga agcggatcaa gaacatagtg
     8761 atttaactgg tagtgaacaa gaagcgcatt ttatagtaga acaagttaaa gatatcttag
     8821 aacatcaaaa agtttttgat atgaaaacag gaagctatag aagtgcgaca tacaaggata
     8881 tcgttattct agaacgcagc tttggacaag ctcgcaattt acaacaagcc tttaaaaatg
     8941 aagatattcc attccatgtt aatagtcgtg aaggatactt tgaacaaaca gaagtccgtt
     9001 tagtattatc atttttaaga gcgatagata atccattaca agatatttat ttagttgggt
     9061 taatgcgttc cgttatttat cagttcaaag aagatgaatt agctcaaatt agaatattga
     9121 gtccgaatga tgactacttc tatcaatcga ttgtaaatta cattaatgac gaagcagcag
     9181 atgctatttt agttgataaa ttaaaaatgt ttttatcaga tattcaaagt taccaacaat
     9241 atagtaaaga tcatccggtg tatcagttaa ttgataaatt ttataatgat cattatgtta
     9301 ttcaatactt tagtggactt attggtggac gtggacgacg tgcaaatctt tatggtttat
     9361 ttaataaagc tatcgagttt gagaattcaa gttttagagg tttatatcaa tttattcgtt
     9421 ttatcgatga attgattgaa agaggcaaag attttggtga ggaaaatgta gttggtccaa
     9481 acgataatgt cgttagaatg atgacaattc atagtagtaa aggtctagag tttccatttg
     9541 tcatttattc tggattgtca aaagatttta ataaacgtga tttgaaacaa ccagttattt
     9601 taaatcagca atttggtctc ggaatggatt attttgatgt ggataaagaa atggcatttc
     9661 catctttagc ttcggttgca tataaagctg ttgccgaaaa agaacttgtg tcagaagaaa
     9721 tgcgattagt ctatgtagca ttaacaagag cgaaagaaca actttattta attggtagag
     9781 tgaaaaatga taaatcatta ctagaactag agcaattgtc tatttctggt gagcacattg
     9841 ctgtcaatga acgattaact tcaccaaatc cgttccatct tatttatagt attttatcta
     9901 aacatcaatc tgcgtcaatt ccagatgatt taaaatttga aaaagatata gcacaaattg
     9961 aagatagtag tcgtccgaat gtaaatattt caattatata ctttgaagat gtgtctacag
    10021 aaaccatttt agataatgat gaatatcgtt cggttaatca attagaaact atgcaaaatg
    10081 gtaatgaaga tgttaaagca caaattaaac accaacttga ttatcaatat ccatatgtaa
    10141 atgatactaa aaagccctca aaacaatctg tttctgaatt gaaaagacaa tatgaaactg
    10201 aagaaagtgg cacaagttac gaacgagtaa ggcaatatcg tatcggtttt tcaacgtatg
    10261 aacgacctaa atttctaagt gaacaaggta aacgaaaagc gaatgaaatt ggtacgttaa
    10321 tgcatacagt gatgcaacat ttaccattca aaaaagaacg catatctgaa gttgagttac
    10381 atcagtatat cgatggatta atcgataaac atattatcga agcagatacg aaaaaagata
    10441 tccgtatgga tgaaataatg acatttatca atagtgagtt atattcgatt attgctgaag
    10501 cagagcaagt ttatcgtgaa ttaccgtttg tagttaacca agcattagtt gaccaattgc
    10561 cacaaggaga cgaagacgtc tcaattattc aaggtatgat tgacttaatc tttgttaaag
    10621 atggtgtgca ttattttgta gactataaaa ccgatgcatt taatcgtcgc cgtgggatga
    10681 cagatgaaga aattggtaca caattaaaaa ataaatataa gatacagatg aaatattatc
    10741 aaaatacgct tcaaacgatt cttaataaag aagttaaagg ttatttatac ttcttcaaat
    10801 ttggtacatt gcaactgtag tattttgatt ttcaaaagaa taaaaaataa tttcgattaa
    10861 gtgcaaagtc cttgtagcag aatgaacaca actcattttc aaaattgtct tacttattta
    10921 tttgttattt gataacgaaa aaagttataa tgtgaattaa gataaagatg aggagttgag
    10981 aatgaatgaa attcttatca ttcaagtata atgacaaaac ttcatatggc gttaaagtaa
    11041 aacgcgaaga tgctgtatgg gatttaacac aagtatttgc tgactttgca gaaggagatt
    11101 tccatcctaa aacattgtta gctggtttac aacaaaatca tactttagat tttcaagaac
    11161 aagtacgtaa agcagttgta gcagcagaag atagcggcaa agctgaagac tataaaattt
    11221 catttaatga cattgaattc ttaccaccag taacacctcc gaataatgtg attgcttttg
    11281 gtagaaatta caaagatcat gcgaacgaat taaatcatga agtagaaaga ttatatgtat
    11341 ttacaaaagc agcgtcatct ttaacaggag ataatgcaaa aattccaaat cataaagata
    11401 ttactgatca attagattat gaaggtgaat taggtattgt tattggtaag tctggtgaaa
    11461 agattccaaa agcattagct ttagattatg tttacggcta tacaattatt aacgatatca
    11521 ctgatcgcaa agcacaaagt gaacaagatc aagcattttt atcaaaaagt ttaactggcg
    11581 gttgcccaat gggtccttat atcgttacta aagacgaact accattacct gaaaatgtaa
    11641 atattgttac aaaagttaac aacgaaatta gacaagatgg caacactggc gaaatgattc
    11701 ttaaaattga tgaattaata gaagaaattt caaaatatgt tgcactacat ccgggagata
    11761 ttattgcaac tggtacacca gcaggcgttg gtgcaggtat gcaaccacca aaattcttac
    11821 aaccaggtga tgaagttaaa gtgactattg ataatattgg aacgctgaca acttatatcg
    11881 ctaaataatt atcatttaaa aagctaacca gtctttatat agattggtta gttttttctt
    11941 gcttttctaa aaagtgttaa agatcattgt ttataatgtt accattttga gacgaaagtg
    12001 aaatattgat attaagaagt agttgat
//
LOCUS       DAKSQE010000082        11858 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.082, whole genome shotgun sequence.
ACCESSION   DAKSQE010000082 DAKSQE010000000
VERSION     DAKSQE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11858
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.082"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(59..1918)
                     /locus_tag="O0V33_001930"
     CDS             complement(59..1918)
                     /locus_tag="O0V33_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HCX8976554.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSNDDETSKDTTSKDIDKADNNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGENSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSTKPTQPNQSNSQPASDDKANQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKTQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNQF
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
     gene            2129..5110
                     /locus_tag="O0V33_001931"
     CDS             2129..5110
                     /locus_tag="O0V33_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCX8976555.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVIRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFADITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     SILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFPKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFAQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLEPVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
     gene            complement(5222..6160)
                     /gene="manA"
                     /locus_tag="O0V33_001932"
     CDS             complement(5222..6160)
                     /gene="manA"
                     /locus_tag="O0V33_001932"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCX8976556.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNDTTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRGSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLAV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(6174..8126)
                     /locus_tag="O0V33_001933"
     CDS             complement(6174..8126)
                     /locus_tag="O0V33_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HCX8976557.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLERVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAP
                     IYQSETNYYSKDLDKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVIVGLIFIFL
                     IGAPISSIFEALTTWLKGMQGANIIILALIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANMIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHILWFILAVIVGSL
                     VTMVTVLLLKRRTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDNTSKHSHLNKPSHVF
                     DQQTIIITDHDMSRNEAIDTLIHQLKVCRYVEHTSHLKNAILEREMESTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWESLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            complement(8203..10077)
                     /locus_tag="O0V33_001934"
     CDS             complement(8203..10077)
                     /locus_tag="O0V33_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCX8976558.1"
                     /translation="MLDRQLKLVHLFLINPSKHLSSDEIAEHINVSNRTVRNDIHVIN
                     SNFMDDVIISIKSKGYQLNTSRYTLDAITDRYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEKDYLLSKTVLTDYFIRIQQWCQKFNIALTIKKKQGIIVDGTDNDITNAIIHLN
                     QLSTGHVNVEDLILNELPDAHRRMMSHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDSKLISQSCIQQINTRLGYDLNEQVINMFSFFIGYHFNKLDLGIER
                     IFIQSYIDRLIELMQQHIHVPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAVDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELSVINTIKLENITHYHFDNVDLLITTHDIPKQTLNILPKHL
                     STIKVAPLFSEDDRHKIRHIVKQKQNPVQAHHHMDTVNFLVVNTEQKPRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVRLVFFLAISQKDTAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE
                     "
     gene            complement(10303..10776)
                     /locus_tag="O0V33_001935"
     CDS             complement(10303..10776)
                     /locus_tag="O0V33_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976559.1"
                     /translation="MKKFLITVVIILLIGALGFAIYAAINHSTSSNHNNEQDNKATHQ
                     KKDTEVNHQKDENTNNTNENQNNNNGVTNGNQSSTPSTVQNNGSTAPANTQPSNPNQS
                     NSNQSNSNHGGSSQPSTNNQNHKQQNTPAPNKSNNANTNESSKQVSPSQPQSSKN"
     gene            11031..11558
                     /gene="isaB"
                     /locus_tag="O0V33_001936"
     CDS             11031..11558
                     /gene="isaB"
                     /locus_tag="O0V33_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HCX8976560.1"
                     /translation="MNQTTKLCAATTLALGTLIGATVVGTASPIHQEAQAATTPYYTY
                     HGYIGHNANFIMDKHFINAIKYDNVTFNGIKLAKTNATKKVEKYDQTFKGVTAKGNEA
                     NQLQFIVKNNISLKDIQKAYGKDLKKENNETKESDSGIFYYQHSKKSLGIWFVVDHNR
                     VVEVTVGHTPYKTSK"
BASE COUNT         3895 a         1874 c         2135 g         3954 t
ORIGIN      
        1 atgtttaagt ccgaactcct tgtttatact tataaatttt acgggtctaa tataatactt
       61 atttacctgt aatatatgat aattcttcag cggcagctgc attgatagtt ctatgagaaa
      121 tgatacctaa tcctttaaca ttggattctg aaataacgat agaaccatca ctgttaactt
      181 tttcaacaaa tgctacatga ccgtaatgtt gatctgcacc aaattgtcca gcctcaaata
      241 caacagcagc atgacgtttt ggtgtatgac ttacttgata atcacggtat tgagctcgat
      301 tattccaatt atgtgcatca cctaaatcac ctgagataga tgtaccaaat tgtttcatac
      361 ggttatatac gtaccaagta cattggccat gtggatatgg catactatca gatacctcgc
      421 ggaaaggttt gaattcatct gatgaatcat cataatcctt gatagaacgt tcatatttat
      481 ctaaatctgg catgcgttca tcgtcaaact gagttaattg atagtgttta ataatactgt
      541 ttaatttctt agcatagttt ggatctgtag catatgtttt agataagtgt gatgttgcat
      601 ctttataaga atcggcttcc gatttccatg ttggtttata aattgttcga ttgccatcaa
      661 taccattttt aataaggtca gagtaatctt ttagtgattc tttcgtgctt ggatattttc
      721 ggaatccagc attaatacta taaaattgat taccatcagc ttctaatgtg ttaaaaggaa
      781 cagaattccc ttcaaaagca cctttgatac cgaataaatt atggtttggt gacttagcta
      841 aagcactacg acctgagtca gattctaaga ttgcttgggc aatcatgaca gacgcataaa
      901 tatcgttatc ttgaccaatg cgatgtgcat ctttagcaat tgatttgaca aattgacgtg
      961 tatcttttga gtcaacaacg ttaaattgtc cactatcatc attgttagat atactaggat
     1021 ctgtttcgaa taatgatgtt gcacgtgtat ccttttgatt aacatcgtta ttgaatgatt
     1081 gagcgggttt agatttatgt ttcaattcat cttgtgttgg taactgtgga ttctttgtat
     1141 tagatttttc atttttgtct tttttagatt gagatgcgta atctttttgt gttttctttg
     1201 catcttcact gtattgatcc aaaatagagt ctaaagccga atctgacatt gattgattat
     1261 ctttcgatga agatttttga tttgctttat cgtcacttgc tggttgacta tttgattgat
     1321 taggttgtgt tggctttgtc gaatttggtt gcttattaga tgtacttggt tttgtgttgt
     1381 ttgatttagg tgctttttga ttgtctgctt tatcttgttt agatgattgc gtatcagtgt
     1441 catttttgat gctattgtca ctgtttttat tcgaatcatt tgttgaattt tcaccattac
     1501 gaggttgttc gtaatcagaa atatccgaat ttaaattgaa caagttttgg attaaagttg
     1561 ttaatgagta attatcatcg tatttatttt tggttagcaa ttggtttata ttggtttgtg
     1621 gtaaattctt ataaataaaa tcaatgatat tgttagagtc tgaagtgctg tcgtctatag
     1681 ttttaaattt tttgtcgtta ttgtcttggt tacttgtatt attgttgtct gctttatcaa
     1741 tatctttact tgtagtatcc ttagaagttt catcgtcatt agattttttt gaatcatgag
     1801 atgttgtctt agctgtagta tctttttgag gtgtatcagc ataagcggta ggtgaaacta
     1861 aagtaggtaa tacgagcgta gttgatagca aataaattaa aattttattt ttaggcatat
     1921 ttcgtattct cccttgaaaa atataataat taagtgtgat aataaactat gatttgttat
     1981 aatttatcgt atgctgaaaa tagttgatag gtatcaatcg actaaatatc ttccagtaaa
     2041 ttgattatac taattcacaa cgcaaaaata aattaattta caaaaaatat ataaaaaata
     2101 tgaataattc ctacatagga gtgtgacaat gaagaacgca tttaaattat ttaaaatgga
     2161 tctgaagaaa gtagctaaga cgccagctgt gtggattatc ttagcaggct tagctatttt
     2221 gccatcgttc tacgcttggt ttaacttatg ggcaatgtgg gatccatatg gcaacacggg
     2281 acacatcaag gtcgcagtcg ttaatgaaga taaaggcgac acaatcagag ggaaaaaagt
     2341 taatgtcggt aatacgatgg ttaatacact caagaaaaat aaaagttttg attggcagtt
     2401 tgtaagtaga gagaaagctg atcatgagat aaaaatgggt aaatattttg cgggtattta
     2461 catcccatct aagtttacac atgaaattac cggaacacta cgtaagcagc ctcaaaaagc
     2521 agatgtagaa tttaaggtga atcagaagat taacgctgtt gcgtctaagc taacagatac
     2581 tggttcgtca gttgtcgtcg aaaaagcgaa tgaacaattt aataaaacag taattcgagc
     2641 attattagaa gaagctaaca aagcaggttt aactattgaa gaaaatgtgc cgacaattaa
     2701 taagataaaa aatgcggtat attcagcaga taaagcttta cctaagatta atgactttgc
     2761 gaataaaatt gtatatttga ataaccacca agcggattta gataaatatg cgaatgattt
     2821 tagaaaacta ggaaattata aaggtgatat tttagatgct cagaaaaaat taaacgaagt
     2881 caatggtgct attccgcaac ttaatgaaaa ggctaagttg atcttagctt taaataatta
     2941 tatgccgaaa attgaaaaag cgttaaattt tgcagctgat gacgtgccag cgcagttccc
     3001 taaaattaat caaggactta acattgcgag tcaaggtatt gatcaagcta atggacagtt
     3061 aaatgatgcc aaaggcttcg tcacacaagt tagaagtaga gtcggtgatt atcaagatgc
     3121 aattcgacgc gcgcaagatt taaatcgaag aaaccagcaa cagattcctc aaaatagcgc
     3181 tgcgaacaac gaaacatcaa atagtgcacc tgcagctggt aatggtgtag catcaacgcc
     3241 accaagtgca ccaagtggcg atactgcacc aaataataat gttacgcaaa ataccgcacc
     3301 aaatagtaat aatgcgcctg tatcgactac accacaaagt acaagcggga aaaaagatgg
     3361 tcaaagtttt gcagatataa caacaacaca agtcagcaca gctaatgaga acacacaaaa
     3421 cattacagat aaagatgtta aatcaatgga agcggcatta acgggctctt tattatcatt
     3481 atcaaataat ttagataccc aagcgaaagc cgcacaaaaa gatagtcagg cattacgtaa
     3541 tatttcgtat ggcattttag catcggacaa gccttctgat tttagagagt ctttagataa
     3601 tgttaagtct ggtttagaat acacaactca atataatcaa caatttatcg atacattaaa
     3661 agagattgag aagaatgaaa atgttgattt atcaaaagaa attgataagg taaaggcagc
     3721 taataatcga attaatgaat cattaaggtt agttaatcaa ttaagcaatg cattaaagaa
     3781 tggtagttca ggaactgctg aagctactaa attactagat caactgtcaa aactagattc
     3841 atcattatca tcatttagag attatgttaa aaaagatctt aacagctctt tagtatcaat
     3901 atcacaacgt attatggatg aattgaataa agggcaaacg gcattgtcta atgttcagtc
     3961 taagctaaat acaattgatc aagtcatcaa cagtggtcaa tctattttaa aaaatggtaa
     4021 aacacgtatc gatcgtttac aaacagtatt accaagtatt gaacaacaat acattagtgc
     4081 tgttaaaaat gctcaagcaa acttccctaa agtgaaaagt gatgtagcga aagcagctaa
     4141 ctttgtacgt aacgatttac cacagttgga gcaacgttta accaatgcga cagcaagtgt
     4201 gaataaaaat ttaccaacgt tattaaatgg ttatgatcaa gcggtaggac tactaaataa
     4261 aaatcagcca caagcgaaaa aggctttatc agatttagct gattttgcac aaaataaatt
     4321 gccagatgtt gaaaaagatc tgaaaaaagc gaataagatt ttcaagaagt tagacaaaga
     4381 cgatgcagtc gataaattaa tcgacacact taagaatgat ttgaaaaagc aagcgggtat
     4441 tattgcaaat cctattaata agaagactgt tgatgttttc ccagttaagg attatggatc
     4501 agggatgaca ccattctata ctgcattatc ggtatgggta ggcgcactct taatggtaag
     4561 cctattaact gttgataata aacataagag cttagagcca gtgttaacga cacgacaagt
     4621 gttcttaggt aaggcaggat tctttataat gcttggtatg ttgcaagcac tcattgtatc
     4681 ggttggagat ttgttaatcc taaaagcagg ggttgagtca cctgtattat tcgtacttat
     4741 aacgattttc tgttcgatta ttttcaactc aatcgtatat acgtgcgtat cattacttgg
     4801 taacccaggt aaagccattg caatcgtatt gcttgtatta caaattgcag gtggtggagg
     4861 aacattccca attcaaacga caccacaatt tttccaaaac atttcaccat acttaccatt
     4921 tacgtatgcc attgattcat tacgtgaaac agtaggcggt attgttccgg aaatcttaat
     4981 tacaaaatta attatattaa cgttatttgg cataggattc ttcgttgtag gtttaatttt
     5041 aaaacctgta acagatccat tgatgaagcg cgtatctgaa aaagttgacc aaagtaacgt
     5101 tacagaataa aaatgaaatc cacacactag ggttctatca ccttaatgtg tggattttta
     5161 tgtttttaga cagaagagat agtaatttct gtcttttatg ggacgggtgt tatcattgct
     5221 attatccagg atgactaact atagcactaa tattaccgac aaagtgaata tcctcgtctt
     5281 ccgtagttaa aataaagcta gaaccttttt ggatgtcata gtgcttatcg tttactgcta
     5341 aagtaccagt accatcgata attgtaacta agcaataagc atgtggttta ttgaatttta
     5401 aatctccatg aatatcccat ttatatactg caaaatattg attatctaca aattgagtta
     5461 cagtgtgtgt gtcgatgtga gttgttatag gagtagtatt tggttcatga ttgcctaatt
     5521 caatcacatc tttactttgc tctaagtgca aatcacgcaa ttgaccattt tgatctcgtc
     5581 tatcatagtc ataaatacgg tatgtcgtat cggatgattg ttgtgtctct aaaattaaaa
     5641 tacccgaacc aatggcatgg acagtgccag caggaacata ataaaagtca ccgggtttaa
     5701 caggtatacg tttgaaaaga ctgtcaaatt catgattatc aatcatgtcg attaacgtct
     5761 gtttattatg tgcatgtacg ccatatataa tttcagcacc tggggctgca tctaaaatat
     5821 accaacattc tgttttacct agttcgcctt catgttttaa agcgtaatca tcatcaggat
     5881 gaacttggac agataattta tcattggcat ctaatacttt agttagcaga gggaaactac
     5941 ctcgtgaatc attatcgaat aattcacgat gttgtgacca aagttgatct agggtcatat
     6001 ccttgtatgg accattgata attgtattag gaccatttgg atgcgcagaa attgcccagc
     6061 attcaccagt tgtgtcatta gggatatcat agttaaatgc ttttaatgca tgaccacccc
     6121 aaattctgtc tttaaaaacg ggttgtaaaa ataatgccat agttaaaact cctctatatt
     6181 ttcattaata agttataaat ttctgtagta ctgtttgcat taattagtga ttggcgtgtc
     6241 tcatcattca ttaacgcttt agataagcgc tgaagtattt ttaaatgtgt atcctgactg
     6301 ttgtttggta cggcaattaa gaatatcaat tgaggtagac taccatctag actttcccat
     6361 ttaacaccat gattattttt cataacagct acaatcggtt gttttacaac atcagacttt
     6421 gcatgtggaa tggccacgtt catgccaata gctgtcgtag actccatttc acgttctagg
     6481 attgcatttt ttaaatgcga tgtgtgctct acataacggc acactttaag ttgatgaatt
     6541 aacgtatcga ttgcttcgtt tcgagacatg tcgtgatcag taataatgat agtttgttga
     6601 tcaaaaacat gtgaaggttt attgagatgt gaatgtttcg aggtgttatc tacattgtca
     6661 acctctgtat catgttgtgt tatatctgta tcatgaagtt gcgtgtgttg cgctggtgca
     6721 tctactgcta taactggtgt gcgacgtttt aataataaga cagtgaccat tgtgacaaga
     6781 ctaccgacaa tcactgctaa gataaaccat aatatatgat caataccacc taatacagct
     6841 acaattgggc cgccatgtgc cactctatct ccaacaccac cgatggctgc aatgactgat
     6901 gcaatcatcg ctccaatcat gttagcaggt ataatgcgta gtgggtcttg agctgcgaaa
     6961 ggaatagcac cttcagtaat accaaatagt cccatagtga aggaagcctt acccatttct
     7021 ctttcggaat gattgaattt atacttttga acaaacgttg ccaaaccaag accgatgggt
     7081 ggtgtacata cagctacggc taccattccc ataacagcat aatttccttc agcaatcagt
     7141 gctgaaccaa ataaaaatgc taccttgtta actggaccac ccatatcgaa ggcgatcatt
     7201 gccccaataa tcagcgcaag tataataata ttagcacctt gcataccttt taaccaggtt
     7261 gttaatgcct caaaaatact cgaaatcggt gcgccaatta aaaagatgaa aattaaccca
     7321 acaataacgg atgaaataat tggaataata ataataggca ttattggtgc catcgctttt
     7381 ggaactttaa tatctttaat ccactttgcg atataacctg ctaagaaacc agcaacaata
     7441 ccacctaaaa atcctgcgcc tgcatcactg ccataaaaac taccgtcagc agcgatagcg
     7501 ccgccaatca taccaggaac aagtccgggc ttgtcagcga tactaacagc gatatatcca
     7561 gctagtattg ggaccataaa tttaaaggct aaactaccaa tgttttcaat ggatttccaa
     7621 aatgaatcat ctgggatgac taatcctttt gatgtcgttt caccgcctag agtcagcgcg
     7681 atggcgataa ggagtccacc aactacgata aaaggaacca taaacgatac accgttcatt
     7741 aaatgttgat acaccatttg aataccactt ttagacttat cgagatcttt cgaataataa
     7801 tttgtttcag attgataaat aggcgcatct tgattaatga tacgttgaat tagacctctc
     7861 ggattatgaa tcccttcgcg aacattttca ttaatcaacc gtttaccaac aaatcgggac
     7921 agatcaactt gtttatcagc tgcaattatg acaccgtcag cttcttcgat gtcttgcgta
     7981 gttaaaacat tttcagcacc aacaccgccc tgtgtctcta ctttaatatc cacacccatt
     8041 tcttttgcta cccgctcaag cttttcttga gccatatatg tatgtgcaat gccatttggg
     8101 catgaggtaa tagctacaat tttcataaaa tcatctcctt ttctatattg taagcggatt
     8161 ctcgatacta aaaaaaagaa taattaccgt tactagtggc aattattctt gtaagtattc
     8221 aaataattgt tgctttaaac tatgatcatc taaactacat aaatggttca ctgaatcatc
     8281 atccaagtta gcaattaatt gcatcatttg ttttgtaaaa gctgtgtctt tttgtgaaat
     8341 tgctaagaaa aagacgagtc tgacatcgtg ttgtcgccat ggaaaaacat cttttgtgcg
     8401 aaaaataagc acatgtgatt gtaaaacttt ttcaggatct ccatgaggaa tcgccataaa
     8461 attacctatg tatgtagaag atgatttctc acgctctaaa gctgattcga tatatccttc
     8521 tacaatcgca tgatgtgctt gtaatatttt ttgagcttct tcaaaaattt gcacagtatg
     8581 tcgtggtttt tgttcagtat ttacgacaag aaaattgaca gtgtccatat gatgatgtgc
     8641 ttgaaccgga ttttgctttt gcttcacaat atgtctgatt ttgtgacggt catcctcaga
     8701 aaataatggt gcaaccttga tagttgaaag gtgcttagga agtatgttta gcgtttgttt
     8761 tggaatatca tgggtcgtta ttaataaatc tacattgtca aagtgatagt gtgttatatt
     8821 ttccaattta atcgtattta tcactgacaa ctcttcggat aagttattta ttttagtttc
     8881 taaaaaattc gacacaccta gaccataata acaagcaatg actacattta attgtgtttt
     8941 ggtacgacgc tcgatggcag cttgaaaatg aatagttaaa aaagcaattt catcttcact
     9001 catctcaata tctgtatcaa ctgctaattt atcaatcgct tcaaaaagtg tgttaaacac
     9061 aaagggatag agttttttaa tctctataac taaaggattg tttaaataaa tgttttgagt
     9121 gatacgtaaa tatgctttac taaaatgatt atataaattt tgttgtaaaa tcgaatcttc
     9181 attgaaaggt acatgaatat gttgctgcat caattcgatt aagcgatcaa tataactttg
     9241 tataaaaata cgttcgatac ccagatcaag cttattaaag tgataaccaa taaagaatga
     9301 aaacatattg atgacttgtt cgtttaaatc ataacctaat cttgtattga tttgctgaat
     9361 gcaagattga gatatcaatt ttgaatcgtg gtttaacgtg tctagctcac ttaaagattg
     9421 cttacgttta atgattaaaa tgagatgaat taaaagctgt tgtatttgaa tatcagttgt
     9481 ttcaatacta tgttgttgaa gtgtctcttg tataatatgc gacatcatcc gtcggtgggc
     9541 atcgggtaat tcatttaaaa taagatcttc aacgttaacg tgtccggttg ataattgatt
     9601 taaatgaata atggcatttg ttatatcatt atcagttccg tcgacaataa tgccttgttt
     9661 tttcttgatt gttaaagcaa tattaaattt ttgacaccat tgttgaatac gaatgaaata
     9721 gtcagtgagc accgttttag acagcaggta gtctttttca agttgctgca atgtctgtga
     9781 tttgttatgc attagcaatt gatatgccat agaaagtaag agcttttctt tatatgattg
     9841 aatatgcgta tagcgatcag ttattgcatc tagtgtataa cggctggtat ttaattgata
     9901 tcctttagat ttaatactaa taatcacatc atccataaaa ttgctgttaa taacatgaat
     9961 gtcgttgcga accgtccgat ttgaaacatt gatatgttca gcaatttcat cggaacttag
    10021 atgttttgat gggtttataa ggaataggtg gactagtttc aattgtctat caagcatata
    10081 tgtatccccc tcctttctaa aaattattgt gaaagcgctt ttaaaggctg ttaacataat
    10141 taaacatgaa tggtcatact cccgtcaatg acattaaaac gaacttttta taagaagttg
    10201 agtgcatatt tattttaaaa ataaagaaca gcagtgagac atttttaata agaaatgaac
    10261 tgactgctgt gtgtactaca gacttgtaat atgaccatgc tattaatttt tagatgattg
    10321 cggttgtgat ggtgatacct gcttagaaga ctcgtttgta ttagcattat tagatttgtt
    10381 tggggccggt gtgttctgtt gtttatgatt ttggttattc gttgaaggtt gactgctacc
    10441 gccgtgattt gaatttgatt gatttgaatt tgattgattc ggattagatg gttgtgtatt
    10501 cgctggtgct gttgaaccgt tattttggac agttgatggc gtagatgatt gattgccatt
    10561 agtaacaccg ttgttgttgt tttgattttc atttgtgttg tttgtattct cgtctttttg
    10621 atgatttact tcagtatctt tcttttgatg ggtagcttta ttatcttgct cattattatg
    10681 atttgacgat gtagaatgat ttattgcagc ataaatggcg aatccaagtg caccaattaa
    10741 caagatgata acaacagtaa taagaaattt tttcaaatgt aacgcctccg tttataataa
    10801 aaatagtcat acattgcatg gcatcatgct ttcaatatcg acaaaatgct gttaactata
    10861 aaacttgaaa tgcggacgat attaatgttt gtgtcagttt ttatgtactt caaataagta
    10921 taacaaaact gttatatatt tataaatata atcaatagta ttttattgta caagcaagtt
    10981 ataataatag taataagatt gaaaaatttg tatggaaaag agagataaat atgaatcaaa
    11041 caactaaact ttgtgcagca acgacattag cattaggtac tttaattggc gcaacagttg
    11101 tagggactgc atcaccaata catcaagaag cacaggcggc aacaacacct tactatactt
    11161 atcatggata tatcggtcat aacgctaatt ttataatgga taagcatttt attaacgcta
    11221 ttaaatatga caacgtgaca tttaatggca ttaaattagc taagacaaat gcgacaaaaa
    11281 aagtagagaa atacgatcaa acttttaaag gtgtaactgc aaaaggaaat gaagcgaatc
    11341 aattacaatt tatcgttaaa aataatatat cactaaaaga tattcaaaaa gcttatggta
    11401 aagatttgaa aaaagagaat aatgaaacga aagaatctga tagcggtatt ttttattatc
    11461 aacattctaa aaagtcatta ggcatttggt ttgttgttga tcataataga gtggttgaag
    11521 ttacagtggg acatacgcca tacaaaacga gtaaataata aagtaaatat aggggctagt
    11581 atgtgaggaa tgatgtcaca tactagtctt ttttgattat atgatggtag ttttcgtttc
    11641 tccatgatat tgaaatatat agatttaatc atgcgcagtt aagtagtcag catatatatt
    11701 tcacgtgttg attaagtatg tgttgtacta attttttgat tgttaataat ttcacaaaaa
    11761 taaagccttg ataaatttta aaagataatt aagtgcaatt ttcaaaattt tatttagtca
    11821 cagataacat ttttaaaaaa gaaaagaatc aataaata
//
LOCUS       DAKSQE010000083        11351 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.083, whole genome shotgun sequence.
ACCESSION   DAKSQE010000083 DAKSQE010000000
VERSION     DAKSQE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11351
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.083"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            68..763
                     /locus_tag="O0V33_001937"
     CDS             68..763
                     /locus_tag="O0V33_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCX8976561.1"
                     /translation="MDVLTIEHLTKKIGNKTILDDVSFKLKRGQIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNEPQFIILDEPMNGLDPKAVRD
                     VRELIVQKAQEGVTFLISSHILSELVKITNSILIINKGKIVTETSEEELKQFKDNDLE
                     NVLLDIIEREDQA"
     gene            765..1535
                     /locus_tag="O0V33_001938"
     CDS             765..1535
                     /locus_tag="O0V33_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8976562.1"
                     /translation="MGTLIKQECFKLFKKKSTFIAPIVFILLMVAQGYIATKYNEIFT
                     PQESFTSAYNGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSRTTMIVSKIITLF
                     IISLIYFVITIIASIVIGSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSL
                     TLLLSSATNSTGVAIAVGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVE
                     KGFSKSTKLELHELFIGNIAYISIFLILVVFIFKKKNI"
     gene            2040..4004
                     /locus_tag="O0V33_001939"
     CDS             2040..4004
                     /locus_tag="O0V33_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HCX8976563.1"
                     /translation="MTQITERELKKKYLDLLSQHFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKDKLSTKELNDLTALVYYPEDKLQL
                     IKCDFQNYGQLNVWYITTIEHLIQLIKYCSSKYTRSKLRRALPEQYVFIVEELLYKNN
                     EFKNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIVEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPVIKRRP
                     NFEMDERLVLEKVNYDTNEITVYGKTYPLKDTCFQTVNRDNPAKLLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPCNGNLLIHGCIPVDENGEMESFEIDGQTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRIVDKELQ
                     RKKIRNTNKGKELQAQIDILKMLMHDRYLD"
     gene            4348..5427
                     /locus_tag="O0V33_001940"
     CDS             4348..5427
                     /locus_tag="O0V33_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976564.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMIVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVVFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHFDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(5532..6125)
                     /locus_tag="O0V33_001941"
     CDS             complement(5532..6125)
                     /locus_tag="O0V33_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCX8976565.1"
                     /translation="MEHIFREGQNGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLAFRGQELLTFIKEAAERYDFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPIEVRSTKKLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFKTRGAQVEEVWVNGHEITETGLTAGQQILEK"
     gene            complement(6147..7115)
                     /locus_tag="O0V33_001942"
     CDS             complement(6147..7115)
                     /locus_tag="O0V33_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCX8976566.1"
                     /translation="MTNNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALMYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNEGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNIALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHDAIEAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEVRPFNTKRQHD"
     gene            complement(7193..7627)
                     /locus_tag="O0V33_001943"
     CDS             complement(7193..7627)
                     /locus_tag="O0V33_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCX8976567.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            7853..8137
                     /locus_tag="O0V33_001944"
     CDS             7853..8137
                     /locus_tag="O0V33_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCX8976568.1"
                     /translation="MSNLEIKQGENKFYIGDDENHALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLVKAVVEHARENHLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            8312..9118
                     /locus_tag="O0V33_001945"
     CDS             8312..9118
                     /locus_tag="O0V33_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCX8976569.1"
                     /translation="MAFHDKTATQVTNIELNVRDLDLMTTFYKNILGISVKSSDDNTT
                     VLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTW
                     QSNQARQNNAQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK"
     gene            complement(9239..9910)
                     /locus_tag="O0V33_001946"
     CDS             complement(9239..9910)
                     /locus_tag="O0V33_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCX8976570.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQDPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            10180..11172
                     /locus_tag="O0V33_001947"
     CDS             10180..11172
                     /locus_tag="O0V33_001947"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCX8976571.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     TMFDHVKKGAILVNAARGAVINTPDLIDAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN"
BASE COUNT         3695 a         1990 c         1822 g         3844 t
ORIGIN      
        1 tgataaaatt aattttatat atataatctt aagattcaga ttatttaata gcaaaggaga
       61 tagtgatatg gatgttttaa caatagaaca tttaacaaag aagataggta ataaaacgat
      121 tctcgacgat gtatcattta agctgaaacg cggacaaata gttggtcttg ttggagcgaa
      181 tggtgcaggt aaaacaactt taatgaaagt tatattaggt tactctagtt tccaaagcgg
      241 gaattttaat gttattaaca gcaaggatag caaaagcaat atcggtgcat tgattgaaaa
      301 tccaggaata tatcctttta tgtctggata tgaaaacttg aagttattga atgaatcaaa
      361 aaacactcaa gatatcgata aaattgtctc acaacttcat atggatgaat acattcataa
      421 aaaagctaaa acgtattctc ttggtatgaa acaaaaatta ggaattgcta tagcattttt
      481 aaatgaacct caatttatta tcttagatga accaatgaat ggcttagatc caaaagctgt
      541 gcgagatgta cgtgaattga ttgtccaaaa agcgcaagaa ggtgtcactt tcttaatttc
      601 gagtcatatt ttaagtgaat tagttaaaat cacaaactct atccttatta ttaacaaagg
      661 taaaattgtt acagaaacat cggaagaaga acttaaacaa tttaaagata atgatttaga
      721 aaatgtatta ctagacatca tagaaaggga ggatcaagca taaaatggga actttaatta
      781 aacaagaatg tttcaaatta tttaaaaaga aatcaacttt tatcgcacct attgtcttta
      841 ttctactaat ggttgctcaa ggttatattg ctacaaaata caatgaaatt tttacaccac
      901 aggaatcttt tacatctgct tataatggtt tttcatggtt tgcattttta ttaattattc
      961 aagcaagtac aatcatttca atggaatttc attacggtac gattaaaaat ttactctatc
     1021 gtgaatattc aagaacaact atgattgtta gcaaaatcat cacattattt attatttctt
     1081 taatttattt tgttattaca attattgctt caattgttat tgggtcttta ttctttaatg
     1141 atttaaatat atttgaaagt agcggtaatc aattatcttt attgaatcaa ttattattag
     1201 ttagtttagg tacatttgtt ggtgtttggt tagttttaag cttaacgttg ctattatcat
     1261 ctgcaactaa ttcaacgggt gtagccattg ctgtaggtat tgttttttat tttgcaagtt
     1321 ctattttagc agttattcaa acggcacttt tagaaaaaat agactggcta aagtggaatc
     1381 ctattaatat gatgaatatt atgcttcaaa cagttgaaaa aggctttagt aagtcgacga
     1441 aattagaact tcatgaattg tttattggta atattgctta tatttctatt ttcttaatac
     1501 ttgtagtatt tattttcaag aagaaaaata tttagtaact taaagtatta gatgaataaa
     1561 tacacacata tttcatcgta attcaaaatt attttcaaat cccttcaccc aaataatggt
     1621 gcagggattt ttgatatgtc agcatgccac tcattctatc tttaatatat ttcaaaatcc
     1681 ccccacttgt aatgtacaaa atatgctcac ttaaacgata atcgtatctc tgccaagcta
     1741 tttcaactct gctttaatat aaaaattatt ctcccggatg ggatgcttat atctctatca
     1801 tgatatttca acgccgcttc aacaacttta tcacttcata tttatgcggg tacacttcaa
     1861 cgctactaat atgaatgata caaccacaga ttctcaacac taaaattcac aatatgttta
     1921 ctctattttt atcaattcaa gcatacttat tgaaaaatgt taacgttttc ttgataatca
     1981 ttgtaagcgt attaatttta caaaccaatg attgaaatat actacaggag tgacacacaa
     2041 tgactcaaat tactgaaaga gaattaaaaa agaaatattt ggatttatta tctcaacatt
     2101 tcgatactcc agaaaagctt gcgactgaaa ttattaattt agaatctatt ttagaattgc
     2161 ctaaaggtac ggagcatttc gtcagcgatt tacatggtga atatgaagct ttccaacacg
     2221 tattacgcaa cggttctggg aacgtgcgag cgaaaattaa tgatattttc aaagacaagt
     2281 tatcaacgaa agaacttaac gatttgactg cccttgttta ctatccagaa gacaaattgc
     2341 aacttattaa atgtgatttt caaaattatg gacaattaaa tgtttggtat attacgacga
     2401 ttgaacacct cattcagcta attaaatatt gttcttcaaa atatacgcgt tcaaaacttc
     2461 gacgtgcctt gccggagcaa tacgttttca tcgtagaaga gttattatat aagaacaacg
     2521 aatttaaaaa taaaaaatct tactacgaaa cacttgtaaa ccaagtgatt gagcttaagc
     2581 aagcggatga tttaattata gggcttgcgt attctgtaca acgcttagtc gtcgatcatt
     2641 tacacgttgt cggcgatatt tatgatcgtg gcccgcaacc agataaaatc atggacacgc
     2701 tgattaatta tcattctctg gatattcaat ggggtaatca tgatgtgctg tgggttggtg
     2761 cgtatgcagg gtctaaagta tgtttagcta atttactacg tatttgtgca cgttatgaca
     2821 atctagatat cgttgaagat gcatacggta ttaatttaag acctttgcta acattagccg
     2881 aaaaatacta tgatgcagat aatcctgctt ttaaacctaa aaaaaggcct gacaaacatg
     2941 aacgtttaac tcaacgcgaa gaaagtcaga ttactaaaat tcatcaagca attgcgatga
     3001 ttcaattcaa actggaaata ccggttatta agcgacgtcc aaactttgaa atggacgaac
     3061 gacttgtact tgaaaaagtt aattatgata ctaatgaaat aacagtatac ggaaaaacat
     3121 atccattaaa agatacatgc ttccaaactg ttaaccgtga taacccagca aagttactac
     3181 ctgaagaaga agaagtcatg aacaaactat tattgtcatt ccagcaatct gaaaaattac
     3241 gtcgtcatat gtctttcttg atgcgtaaag gctcacttta cttaccttgt aatggcaatt
     3301 tactcattca tggttgtatt ccagttgatg aaaatggtga gatggaatca ttcgaaattg
     3361 atggtcagac atacagtggc caagagttat tagatgtgtt tgaatatcat gtccgtaaat
     3421 catttgatga aaaagaaaat actgatgact tatccactga tttagtttgg tatttatgga
     3481 ctgggaaata ctcttcacta tttggtaaac gtgcgatgac tacgtttgaa cgatacttta
     3541 ttgcagataa agcttctcat aaagaagaaa agaatccgta ctatcatctt cgtgaagatg
     3601 tgaatatggt tcgtaaaatg ctcagtgatt tcggattaaa tccagatgaa ggacgcatta
     3661 ttaatggtca cacaccagtg aaagaaatta atggcgaaga tcctatcaaa gctgatggaa
     3721 agatgcttgt cattgatggc ggcttttcaa aagcttatca gtcaacaact ggcattgcag
     3781 gctatacatt gttgtataat tcattcggaa tgcaactcgt tgcacatcaa caatttaacg
     3841 caaaagaaaa aattctttcc gaaggtatcg acgaactgtc aattaaacgg attgttgata
     3901 aagaattaca acgtaaaaaa atccgcaata caaataaagg taaagagctt caggctcaaa
     3961 tagatatttt gaaaatgtta atgcatgatc gttacttaga ctaaagacga cttttgatgc
     4021 acgtgtcgta cacttattta gaggtgctgt gtttacgcat tggctacgca aacgcgatgg
     4081 taaataggcg acctagctac accgaaaaaa tcccctcact attacgagta gtgaggggat
     4141 tggtgtatct atgctagttc cttgttaact gtattattac aacattacag taaaaaaatg
     4201 caaaattaac taataaatta ataaaatgat cgacgccctt gcttctactc caatttaaca
     4261 aaatactata ttagtactat atttttcatt cacatgtatg ttacattttc tatattactt
     4321 aaaattacga agggacattt ttaaatcatg cgcatcttaa aagagtccat tattgtggca
     4381 tttgcctttg ttggtgttgt cgttggtgcc ggctttgcta ctggtcaaga aattttccag
     4441 tttttcacaa gtcatggcgc gtatagcatt tcaggcatta ttgtgacagg actattgatt
     4501 actttaggtg gaatgattgt catgcataca ggtcatcatc taaagtctag aaatcattct
     4561 gattcaatta actatttctt atatccctct attgcaagag gttttgatat tattttgaca
     4621 atgtttatgt tgtctttagc tattattatg actgcaggtg gcgcgtcaac cattcatcaa
     4681 agtttcaact taccgtattg gctgagcgca ctcatattag tcgtgtttat tttagcaaca
     4741 ctgtttctaa aattcgatcg tttaattgct gtgcttggcg gtgttacacc atttttaatt
     4801 gcgattgtca ttatgattgc ggtctactat ttcacaacga gtcattttga ttttactgcc
     4861 gctaataatg atgctcagat tcataagcag aaatcattat cacctggatg gtggtttgat
     4921 gcgattaact atgcaagctt gcaaattgct gctgccttca gcttcttatc agtgatgggt
     4981 agtaaagtta agtatcgtga ctcaacgtta tacgggggct tgattggcgg tttaattatt
     5041 acatttttac tcatgatgat taacctaggt ttaatttctc aattcgataa aattaaacac
     5101 gtcgatttac ctacattaaa attagcgaca caaatgtctc cgtcaatcgg tattattatg
     5161 tctgtcatta tgatacttgt catctacaat actgttgttg gattaatgta tgcatttgcg
     5221 tcacgtttca gcgttccatt tagcagacgt tacttcatca ttattattac aatggctgtc
     5281 atcacttata ttagtacatt tatcggtttc atttcattaa ttggaaaagt attccctatt
     5341 atgggattgt tcggtttcat cttactcata cctgtactct ataaaggttt aattaagcgt
     5401 attaccggca aatctcatat cgattaaaat atctatacta ttgtaaaatt tagtacaaca
     5461 cttcaaaatc aaaaaagcgg acagatgaaa agtaaaaatc ttttccatct gtccgcttct
     5521 taatagaact gttatttctc aagtatttgt tgaccagccg ttaatccagt ttctgtaatt
     5581 tcatgaccat tcacccaaac ttcttcgact tgtgccccac gtgtcttaaa caagttaatg
     5641 acttgttcac ttgcagctaa tggcactatt ggatcatgtt tccccataga aagcaacaca
     5701 ctgacatctg ataacttctt tgttgacctt acttcaattg gatataacgg tgcatataac
     5761 aatgcttttt taaatggtgc ttctgaacgc aacattaagt taatcgctat atttgatcca
     5821 tttgaaaatc caacaagtac agccttttca atatcaaaat cataacgttc agcagcttct
     5881 ttaataaacg ttaacaattc ttgtccccga aatgccaaat cttcttcatc ataaacacct
     5941 tcaccaagac gtttgaaata acggttcatc ccattttctg aaacttgtcc tctaatactt
     6001 aacaagtgat aattttcatt caatgcttcg cctaatggta ataaatcgaa ctcatcacca
     6061 cctgtaccat gcaataatat tagcgtcggc gcaccatttt gtccttctct aaaaatatgt
     6121 tccatcacaa atccctctca ttccaattaa tcatgttgac gcttcgtatt aaaaggtcta
     6181 acttccgatt ctatatattc tcttttattt tctaaaaatg gtggtaagga taacccttct
     6241 cctaatgttt cataaggttc atcttccata aaccctggtc catctgttga aatttctatt
     6301 aaaatatgcc ccacacgtgc atataatgct tcaaaataaa aacgattaac gatgcctgag
     6361 ttgttaatac ctacctcttt atatttcgtt gcccacgctt ctattgcatc atgatctttc
     6421 acacgaaatg acacatgatg tacctcacca taaccttgac gtgctgctgg ccctttatca
     6481 tcttttatta agattacctg gccaccattg cctccttcgc caacttcaag taatgcgata
     6541 ttatcttcat gcgcaatagt tgtcataccg taaacagtct ctaaaatatt tttaaagtcg
     6601 tcaaaataac ttactttaat ttcaatgggg cctaatccat aaatcgcttt gtctactgga
     6661 accggtccat tcttccaagg tacaccaggt gccacccctt catttaactc atctgaaatt
     6721 aattgataca cttggccatc gacttcttca aatggcaaca cttttttacc aaataattct
     6781 tgaatacctt catgtttaac accaaattca tcaaagcgct gttcataata cattaacgcg
     6841 tcatcgttag gcactctaaa agacggtctt gtaatggaat ttgttcctgc ctgccctttt
     6901 gtaatatttg gaaaatcaaa gaacgtcatg tctgtacctg ccgaaccaac gtcatctgca
     6961 aaaaatgtat gatacgtata aatatcatct tgattgactg tctttttaac taaacgcatt
     7021 cctaatactt ctgtaaaaaa tttataatta cgttctgcat catctgtcat tgctgtgaca
     7081 tggtgaatac ctagtaattc atgattattt gtcattttat ataccctctt tatattttta
     7141 gtgtatttat ctcgaaaccg agatatttta atgtaaaatt ttagtgcacg ccttacactt
     7201 ctgtagattg tgcacttagt ttcttaaacg cttgttgtaa gattgttaat tcatcctttg
     7261 ttaacacatc aaacgctttt gttaatgtct cagcatgctt agggaaaata tctgccattt
     7321 ggctttgacc tttttcagtt aaacaagcca tatatacacg tttatcatct ttatcctttt
     7381 cacgtgtaat ccaacctttg tcctctaatt gacttacaac atatgaaatg ctgctacttg
     7441 cgattaatac gcggtctcta atacgttgaa ttggttgcgg acctttatta taaagcaact
     7501 cgagcactgc aaattcagta atatttaagc cgtatcgctt tacgtcttct tttgtaattt
     7561 gctctaatgt gtctaacgcc ctattcattc cgacaaagac attgagcgat tgtttcgttc
     7621 gatccatatc atcaccaact ttttaattta tttcgatttc gagataaatt atatagctga
     7681 atgaatttaa tgtcaagata cgtgttttca tctttatttc aaattgcact taataaaaac
     7741 gactacatag atgtggcaat gtcgtcacat gactgtcatg cacctttgac ttcgtttaag
     7801 ttaaaataga atagggaaat actataatca atcatgatag gagttgatat gtatgagtaa
     7861 ccttgaaatt aaacaaggcg agaacaaatt ctatattggt gatgatgaaa atcatgcttt
     7921 agctgaaatc acataccgtt ttgtggataa taatgaaatt aacattgatc atacaggcgt
     7981 atctgatgaa ctcggtggtc aaggtgttgg caaaaaacta gttaaagcag ttgttgaaca
     8041 tgctcgcgaa aatcatttaa aaatcattgc ctcatgttca tttgccaaac atatgttaga
     8101 aaaagaagat tcatatcaag atgtgtatct tggttaaata aacctcgcga tggtgcgcca
     8161 gtgccatcgc ttttttattt gttcgtatca atataaaatt gcgaaacatc atgttatttt
     8221 gcatttcaaa ctttttgctg tcaattcatt ttcttttatg gtatactttt tatactaact
     8281 tatagaaaaa tagaaaacga ggcgattcat aatggcattt cacgataaaa cagcaacaca
     8341 agtgacaaac attgaactga acgtaagaga cttagactta atgacaacat tttacaaaaa
     8401 cattttagga atatccgtta aaagttctga cgataacaca accgtattat ctgttgggac
     8461 tggtggccat actctaacgt tacatttatt agaagacggc cgtcagactt ccccacgaga
     8521 agcaggactt tttcatatag catttttatt accaactact gaggatctag ctaacttctt
     8581 atatttcgtg gcacaaaaaa atatgggcat cggcgctggt gatcatttag taagtgaagc
     8641 tttatatttc aacgaccccg aaggtaatgg tattgaagtc tatcgcgata gaccttcatc
     8701 ttcatgggaa tggcaaaatg gcaaagttaa aatggatact ttagaagttg atagccaaac
     8761 cttattaaca catcgtactg atgaaggttg gcaaggaatg ccagcaaaag gcatgattgg
     8821 acacttacat ttaaaaacac atgacttaga cgcagcatat caatgttaca ttgaacaatt
     8881 aggattccaa catgtgtctg attttccacg tgcgctattt atgtcaacga atcattatca
     8941 ccatcatatt gctgctaata cttggcaatc caatcaagca agacaaaaca atgcacaaag
     9001 ttatggatta acacatgttg atatttatca acccgacgcc tcaacacata catttacggc
     9061 accagaaggg tttgacatca cagttcattc aaatactgac cttgttccag aaaaataatc
     9121 atccatactt tattaataca attttttcac gcaaaagcct gcgattcatt tatgtaatcg
     9181 caggcttttt ttattgctaa ctttttaatt ttatatattt agacatactg tattctattt
     9241 attcttttgg tccaacccat tcaataacat cttcatatgc ttggcgtgtt ttatttttcg
     9301 gtggatcttg ttgtctgtag ccaaatgcaa ccatcactga taaaccaaat tgctcagtat
     9361 ctaagatacc tttatttgct aaaatgtctg tcactgtatc cagactaaaa ccttccatcg
     9421 gacatgaatc aatacctaac aatgcggctg tcgtcatcat attgcctaat gcgatatacg
     9481 tttgtttact tgaccagtca tacaaggctt gatcattatc agaaatatgg aaatctgctt
     9541 ggaatgcatc gaatttttgt tcaacagctg gaatcgtttg tgcctcatat tttttaatat
     9601 ctcttaacat atgttgtaca tacggtgatc ttgacgttac atttttacgc gcaaaaatta
     9661 gcacaaaatg gctcgctgta tctaattgtt tcgctgcgcc ccaactgtgc gcttttaatt
     9721 catcacgtaa cgcttgatct tgaatcacga caaacttcca aggttctaac ccaagagaac
     9781 ttggagacaa tctacctgac tctaatattg tttcaaaatc ttctttcgaa actttctttt
     9841 gtggatcgaa ttgcttagta gcatgtctga aatgaaatgc atccataatt gtttgattca
     9901 tattgctcat tgaacatagc ctccaatttt tatatttatt gctatacaaa ttatacttca
     9961 tgttttaaaa agactaaagt aatccgtttg caattcacta taacttgtgc acatgttata
    10021 agttttctgc aataaaacac acttttgaat tttggaaaat ttagtgttat aatatatttg
    10081 tgatattttt cacaattaaa ttgtggcgtg attttaatgt gcataatctt taatttcact
    10141 catttacttt tcaatctttt aaaagcgagg tttttaataa tgacaaaaat tatgttcttt
    10201 ggtacgcgtg attatgagaa agagatggca ttaaattggg ggaaaaagaa taatgtcgaa
    10261 gtaactactt ctaaagagct attatcaagt gctacagtcg atcaattaaa agattacgat
    10321 ggcgtaacta caatgcaatt tggtaagtta gaaaatgacg tttatcctaa attagaatct
    10381 tacggtatta aacaaattgc acaacgtact gctggatttg atatgtatga tttagattta
    10441 gctaaaaaac acaacattgt gatatctaac gttcctagtt attcacctga aacaattgca
    10501 gagtattctg tttctatcgc cctacaatta gtgcgtcgct tcccagatat tgaacgccgt
    10561 gtacaagcac atgattttac ttggcaagca gaaatcatgt ctaaaccagt taaaaatatg
    10621 actgttgcaa ttatcggtac gggtcgtatc ggtgctgcta cagctaaaat atatgcagga
    10681 tttggtgcta caattacagc ttatgacgct tatcctaata aagatttaga ctttttaact
    10741 tataaagata gtgttaaaga agctattaaa gatgccgata ttatttcttt acatgttcca
    10801 gcgaacaaag aaagctatca tttattcgat aaaacaatgt ttgatcatgt gaaaaaaggt
    10861 gcaatcttag ttaacgcagc acgtggtgca gtcatcaata cacctgattt aatcgatgca
    10921 gtgaacgatg gtactttgtt aggtgctgcg attgatactt atgaaaatga agcagcatac
    10981 ttcacaaatg actggactaa caaagacatt gacgataaaa cattattaga gttaatcgaa
    11041 catgaaagaa ttttagtaac accacatatt gctttcttct ctgatgaagc agtacaaaac
    11101 cttgttgaag gtggtttaaa cgcagcatta tctgtcatta acactggtac atgtgaaaca
    11161 cgtttaaatt aatatatatt taaacaggct gggacataaa tccctaaaaa acagcagtaa
    11221 gataattttc aattagaaga tatcttactg ctgttctata tttatacaat acttcgtatt
    11281 gaatggcttc gctatgccca tctggcacat tactgtaaaa ttctataaat agaatttttg
    11341 atgatgggtc c
//
LOCUS       DAKSQE010000084        11296 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.084, whole genome shotgun sequence.
ACCESSION   DAKSQE010000084 DAKSQE010000000
VERSION     DAKSQE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11296
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.084"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            21..2726
                     /gene="acnA"
                     /locus_tag="O0V33_001948"
     CDS             21..2726
                     /gene="acnA"
                     /locus_tag="O0V33_001948"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCX8976572.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSKLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRGAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGSVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            2907..3374
                     /locus_tag="O0V33_001949"
     CDS             2907..3374
                     /locus_tag="O0V33_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCX8976573.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQALNNEGKIVEIMDGIDSL"
     gene            complement(3583..3879)
                     /locus_tag="O0V33_001950"
     CDS             complement(3583..3879)
                     /locus_tag="O0V33_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HCX8976574.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVIAEKDLWYFEDDHIIVNVVDHEDEISYSKK"
     gene            complement(4257..4865)
                     /gene="plsY"
                     /locus_tag="O0V33_001951"
     CDS             complement(4257..4865)
                     /gene="plsY"
                     /locus_tag="O0V33_001951"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCX8976575.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLPVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            5071..7062
                     /gene="parE"
                     /locus_tag="O0V33_001952"
     CDS             5071..7062
                     /gene="parE"
                     /locus_tag="O0V33_001952"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCX8976576.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     YHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTDKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            7062..9464
                     /gene="parC"
                     /locus_tag="O0V33_001953"
     CDS             7062..9464
                     /gene="parC"
                     /locus_tag="O0V33_001953"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCX8976577.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDSSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFISNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVYEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSYNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITIITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            9714..11174
                     /locus_tag="O0V33_001954"
     CDS             9714..11174
                     /locus_tag="O0V33_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCX8976578.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCFVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
BASE COUNT         3924 a         1640 c         2178 g         3554 t
ORIGIN      
        1 gtatcaaggg ggatcattaa atggctgcaa attttaaaga gcaatcaaaa aaacattttg
       61 acttgaatgg ccaaagttat acttactatg atttaaaagc tgtagaagag caaggtatta
      121 ctaaagtttc gaaattacct tattcaattc gtgttttgtt agaatcttta cttcgtcaag
      181 aagatgattt tgtaattaca gacgatcata ttaaagcttt aagtcagttt ggaaaagatg
      241 gaaatgaagg cgaggtacca tttaaacctt ctcgtgttat tttacaagat ttcacaggtg
      301 taccagccgt agttgattta gcttctttac gtaaagcaat ggatgacgtt gggggagata
      361 ttactaaaat taatccagaa gtaccggtgg atttagttat tgaccactca gttcaagtgg
      421 atagctatgc aaacccagaa gcacttgaac gtaatatgaa attagaattt gaacgtaact
      481 atgaacgtta tcagttttta aattgggcaa cgaaagcatt tgataattac aatgcagttc
      541 ctcctgcaac tggaatagtt caccaagtta acctagaata tttagcaagt gttgtacatg
      601 ttcgtgatgt agatggtgaa aaaactgcat ttccagatac attagttggt actgattcac
      661 atacaacaat gataaatggt attggcgtac taggatgggg tgttggtggt attgaagctg
      721 aagctggaat gcttggacaa ccttcttatt tcccaattcc agaggttatt ggtgtacgac
      781 tagtaaattc attaccacaa ggcgcaacag caactgattt agcgttgaga gtaactcaag
      841 agctacgtaa aaaaggtgtt gttggcaaat ttgtagagtt ctttggtcca ggtgtacaac
      901 atttaccact agcagaccgt gctacaatcg caaacatggc acctgagtat ggagcaactt
      961 gcggattctt cccagttgat gatgaatctc ttaaatatat gaagttaact ggtagatcag
     1021 acgaacatat cgcgcttgta aaagaatatt tgaaacaaaa ccatatgttc tttgatgttg
     1081 aaaaagaaga tcctaattat acagatgtta tcgaattgga tttatcaaca gttgaagcat
     1141 cgctttcagg accaaaacgt cctcaagatt taattttctt aagtgatatg aaatcatcat
     1201 ttgaaaattc tgtaacagct ccagcgggca accaaggaca cggtttagat aaaagtgaat
     1261 ttgataagaa agcagaaatt aacttcaaag atggatcaaa agctacaatg aaaacaggtg
     1321 atattgcaat agcagcaatt acatcatgta caaatacatc taacccttat gtaatgttag
     1381 gtgcaggttt agttgctaaa aaagcagttg aaaaaggctt gaaagttcct gaatacgtta
     1441 aaacttctct agcaccagga tcaaaagttg ttaccggata tttaagaggt gctggcttac
     1501 aaccctattt agatgattta ggcttcaact tggttggtta tggatgtaca acttgtattg
     1561 gtaattcagg tcctttatta ccagaaattg aaaaagcgat tgctgatgag gacctattag
     1621 tgacatctgt attatctggt aaccgtaact ttgaaggtcg tatccatcct cttgttaaag
     1681 ccaattacct agcttcacca cagttagttg ttgcttatgc attagctgga tcggttgata
     1741 ttgatttaca aaatgaacct attggtaaag gtaatgacgg tgaagatgta tatttgaaag
     1801 atatttggcc atcaattaaa gaagtttcag ataccgttga tagtgttgta acacctgaat
     1861 tatttattga agaatataat aacgtataca ataacaacga attatggaat gagattgatg
     1921 taactgatca acctctatat gactttgatc ctaattcaac atacattcaa aatccatcat
     1981 tcttccaagg attatctaaa gaaccgggta cgattgttcc gttaaatggt ttacgtgtta
     2041 tgggtaaatt cggtgattct gtgacaactg accacatctc tccagcaggt gcaattggta
     2101 aagatacgcc agctggtaaa tatttacaag atcaccaagt gcctattcgt gaatttaatt
     2161 catatggttc aagacgtggt aatcacgaag taatggttcg aggtacgttt gctaatatac
     2221 gtattaaaaa ccaattagcg ccaggtactg aaggtggttt tacaacttat tggccaacaa
     2281 atgaagtaat gcctatcttt gatgctgcaa tgaaatataa agaagatggt acaggtttag
     2341 ttgtattagc tggtaacgat tatggtatgg gttcatctcg tgactgggca gcaaaaggta
     2401 caaacttatt aggtgttaaa acggttattg cacaaagtta tgaacgtatc catcgttcaa
     2461 atttagttat gatgggtgta ttaccattag agtttaaaaa aggtgaatca gctgattctc
     2521 ttggtctaga tggtacagaa gaaatttctg ttaatattga tgaaaatgtt caaccacatg
     2581 actacgtcaa agttactgct aagaagcaag atggtgattt ggtagaattt gacgctatgg
     2641 ttcgttttga ctcacttgtt gaaatggatt actatcgcca cggtggtatt ttacaaatgg
     2701 ttttaagaaa taaattagcg caataaaaaa taggtatcac agtaaatttt ttatcggtat
     2761 ttgaggcact ttgatagtgc tctaaaaata gctctataaa ctttatgatt taataggtcg
     2821 gttcaaaacc tttgtgttct tggccggcct cttcttgtat tttatgttag aatgagatat
     2881 aaagttaaac tgaaagaagg cttaaaatga tatatagtat tacagaaata gaagcgcgtt
     2941 atgctgaaac tgataagatg ggtgtaattt atcacgggaa ttatgcaact tggtttgaag
     3001 ttgcgcggtt ggattatata tcgaagttag gttttagtta tgctgatatg gaaaaacaag
     3061 gaatcatttc acctgtgact gacctcaatg tcaattataa aaagtctatt ttttatccag
     3121 aaaaagttaa agttaaaact tgggttgaaa aatattcgag attacgttca gtgtataaat
     3181 atgagatttt taatgaaaaa ggtgaacttg caactacagg ttccacagaa ttgatttgca
     3241 ttaaagaaga tacttttaag cctatacggt tggatcgtta tttcccagat tggcatgaag
     3301 cctatagtaa agtgcaagcg ctcaataatg aagggaaaat agtagagata atggatggta
     3361 ttgattcttt ataagttact ataaatgttt ggagaggcaa tattaatatt taatctataa
     3421 aaaatagtca atacgttaac attaacataa ttagcgtgaa gataagctaa tatatatagc
     3481 aaaaacgaac ttgttcatac aacatcagat gtgctaaacc aaagtacaac ttccgaggtt
     3541 cagtacacat cagaatgtgt aaacaagttc gttttatttt agttattttt tggaataaga
     3601 aatttcatct tcgtgatcaa ctacatttac aataatgtgg tcatcttcaa agtaccacaa
     3661 atctttttcc gctatgacaa catttaaatc gtcatattgt tgttcatagc caatatcaac
     3721 atcttccttt ggttcaactg taaaagcagg actaaatcct tgcttgagtt ggaattcgcc
     3781 accatatctt acaaaaaaca cgagcacttt attattttca ggtaactcaa gttcattttt
     3841 aaaccaagtt actgctgcat cagtaagttc tatttgcata acaatgacct cctatattta
     3901 ttacagaatg ggagtgggcc caaactcgta ttgtttgtgg aatttctttt cgaaattctc
     3961 tgtgttggag cccaccccaa cctgcacatt attgtaagct gaatttccat aagcttctat
     4021 gttgggaccg ccgaccagaa ttgaaaaaag ctagttataa gcgcatttcg ttctgtcaac
     4081 tacagctaat ataacattgt ggaacctatg acattgattt atgtctcggt ctcaaatgtt
     4141 cttgtaaaaa aactaagtat agttgaattt taagttaaaa aaattgtttt tgcaaaaata
     4201 aaataatcca tcaactgcaa aaaaatatgc aatgatggac taatgtcaaa aaattgttac
     4261 atccatttta ttttaggttc ttcgcctcta aaaatccttg cgatattaga gcgatgtcta
     4321 attatcaata tgattgaaac taagaaacta acgactaata aaatatagtc ttgaatgata
     4381 agcgagccaa tcacacagca aattgctgca acgatacttg ctaaagaaac atatttaaaa
     4441 atcttcaata caataaagaa gataattgca agtattagta atagtatcgg gttgactccc
     4501 aagacgacac ctgcactagt tgcaacagct ttgccacctt ggaattttaa ataaactggg
     4561 taaacgtgtc caagtatagc gaataagcct acaattaaac catttgtaaa aaaagtacta
     4621 atagggccat ctgcgtgaac tggtaaccat aaagggaaga aaacagttat gaaccctttg
     4681 aaaatatcta gaaatgttac caagaatcct gcaggacgac ctaatactct aaagctatta
     4741 gtagcgccag tattaccact accaaattgt ctaatatctt ttttgaaaaa taattttcca
     4801 attacgaatc cacttgggaa agcgccgata agataactta gtagtaacat gacgattatc
     4861 atcataaata ttacacatcc tttaatatct taggactatt ttatcatatt cgtttaaatt
     4921 acggctaaaa tttttaaaaa cggggattaa tatatggaat taagctatga aagttaattg
     4981 acacttgcat tttacgctga tttatataag aataactatt gtatagtttt aaaaacgaac
     5041 gtacgtttgc aggaggcgaa atcattggca atgaataaac aaaataatta ttcagatgat
     5101 tcaatacagg ttttagaggg gttagaagca gttcgtaaaa gacctggtat gtatattgga
     5161 tcaactgata aacggggatt acatcatcta gtatatgaaa ttgtcgataa ctccgtcgat
     5221 gaagtattga atggttacgg taacgaaata gatgtaacaa ttaataaaga tggtagtatt
     5281 tctatagaag ataatggacg tggtatgcca acaggtatac ataaatcagg taaaccgaca
     5341 gtcgaagtta tctttactgt tttacatgca ggaggtaaat ttggacaagg cggctataaa
     5401 acttcaggtg gtcttcacgg cgttggtgct tcagttgtaa atgcattgag tgaatggctt
     5461 gaagttgaaa tccatcgaga tggtaatata tatcatcaaa gttttaaaaa cggtggttcg
     5521 ccatcttctg gtttagtgaa aaaaggtaaa actaagaaaa caggtaccaa agtaacattt
     5581 aaacctgatg acacaatttt taaagcatct acatcattta attttgatgt tttaagtgaa
     5641 cgactacaag agtctgcgtt cttattgaaa aatttaaaaa taacgcttaa tgatttacgc
     5701 agtggtaaag agcgtcaaga gcattaccat tatgaagaag gaatcaaaga atttgttagt
     5761 tatgtcaatg aaggaaaaga agttttgcat gacgtggcta cattttcagg tgaagcaaat
     5821 ggtatagagg tagacgtagc tttccaatat aatgatcaat attcagaaag tattttaagt
     5881 tttgtaaata atgtacgtac taaagatggt ggtacacatg aagttggttt taaaacagca
     5941 atgacacgtg tatttaatga ttatgcacgt cgtattaatg aacttaaaac aaaagataaa
     6001 aacttagacg gtaatgatat tcgtgaaggt ttaacagctg ttgtgtctgt acgtattcca
     6061 gaagaattac tacaatttga aggacaaacg aaatctaaat tgggtacttc tgaagcaaga
     6121 agtgctgttg attcagtagt tgcagacaaa ttaccattct atttagaaga aaaaggacaa
     6181 ttgtctaaat cacttgtaaa aaaagcaatt aaagcacaac aagcaaggga agctgcacgt
     6241 aaagctcgtg aagatgctcg ttcaggtaag aaaaacaagc gtaaagacac tttgctatct
     6301 ggtaaattaa cacctgcaca aagtaaaaat acagataaaa atgaattgta tttagtcgaa
     6361 ggtgattctg cgggaggttc agcaaaactt ggacgagacc gcaaattcca agcgatatta
     6421 ccattacgtg gtaaggtaat taatacagag aaagcacgtc tggaagatat ttttaaaaat
     6481 gaagaaatta atacaattat ccacacaatc ggggcaggcg ttggtactga ctttaaaatt
     6541 gaagatagta actataatcg tgtaattatt atgactgatg ctgatactga tggtgcgcat
     6601 attcaagtgc tattgttaac attcttcttc aaatatatga aaccgcttgt tcaagcaggt
     6661 cgtgtattta ttgctttacc tccactttat aaattggaaa aaggtaaagg caaaacaaag
     6721 cgagttgaat acgcttggac agacgaagag cttaataaat tacaaaaaga acttggtaag
     6781 ggcttcacgt tacaacgtta caaaggtttg ggtgagatga accctgaaca attatgggaa
     6841 acgacgatga acccagaaac acgaacttta attcgtgtac aagttgaaga tgaagtgcgt
     6901 tcatctaaac gtgtaacaac attaatgggt gacaaagtac aacctagacg tgaatggatt
     6961 gaaaagcatg ttgagtttgg tatgcaagag gaccaaagta ttttagataa ttctgaagta
     7021 caagtgcttg aaaatgatca atttgatgag gaggaaatct agtgagtgaa ataattcaag
     7081 atttatcact tgaagatgtt ttaggtgatc gctttggaag atatagtaaa tatattattc
     7141 aagagcgtgc attgccagat gttcgtgatg gtttaaaacc agtacaacgt cgtattttat
     7201 atgcaatgta ttcaagtggt aatacacacg ataaaaattt ccgtaaaagt gcgaaaacag
     7261 tcggtgatgt tattggtcaa tatcatccac atggagactc ctcagtgtac gaagcaatgg
     7321 tccgtttaag tcaagactgg aagttacgac atgtcttaat agaaatgcat ggtaataatg
     7381 gtagtatcga taatgatcca ccagcggcaa tgcgttacac tgaagctaag ttaagcttac
     7441 tagctgaaga gttattacgt gatattaata aagagacagt ttccttcatt tcaaactatg
     7501 atgatacgac gctcgaacca atggtattgc catcaagatt tcctaactta ctagtgaatg
     7561 gttctacagg tatatctgca ggttacgcga cagatatacc accacataat ttagctgaag
     7621 tgattcaagc aacacttaaa tatattgata atccggatat tacagtcaat caattaatga
     7681 aatatattaa aggtcctgat tttccaactg gcggtattat tcaaggtatt gatggtatta
     7741 aaaaagctta tgaatcaggt aaaggtagaa ttatagttcg ttctaaagtt gaagaagaaa
     7801 ctttacgcaa tggacgtaaa cagttaatta ttactgaaat tccatatgaa gtgaacaaaa
     7861 gtagcttagt aaaacgtatc gatgaattac gtgctgacaa aaaagtcgat ggtatcgttg
     7921 aagtacgtga tgaaactgat agaactggtt tacgaatagc aattgaattg aaaaaagatg
     7981 tgaacagtga atcaatcaaa aattatcttt ataaaaactc tgatttacag atttcatata
     8041 atttcaacat ggtcgctatt agtgatggtc gtccaaaatt gatgggtatt cgtcaaatta
     8101 tagatagtta tttaaatcat caaattgagg ttgttgcaaa tagaacgaag tttgaattag
     8161 ataatgctga aaaacgcatg catatcgttg aaggtttgat taaagcgttg tcaattttag
     8221 ataaagtaat cgaattgatt cgtagctcta aaaacaagcg tgacgctaaa gaaaacctta
     8281 tcgaagtata cgagttcaca gaagaacagg ctgaagcaat tgtaatgtta cagttatatc
     8341 gtttaacaaa cactgacata gttgcgcttg aaggtgaaca taaagaactt gaagcattaa
     8401 tcaaacaatt acgtcatatt cttgataacc atgatgcatt attgaatgtc atcaaagaag
     8461 aattgaatga aattaaaaag aaattcaaat ctgaacgact gtctttaatt gaagcagaaa
     8521 ttgaagaaat taaaattgac aaagaagtta tggtgcctag tgaagaagtt attttaagta
     8581 tgacacgtca tggatatatt aaacgtactt ctattcgtag ctataatgct agcggtgttg
     8641 aagatattgg tttaaaagat ggtgacagtt tacttaaaca tcaagaagta aatacgcaag
     8701 ataccgtact agtatttaca aataaaggtc gttatctatt tataccggtt cataaattag
     8761 cagatattcg ttggaaagaa ttggggcaac atgtatcaca aatagttcct atcgaagaag
     8821 atgaagtggt tattaatgtc tttaatgaaa aggactttaa tacagatgca ttttatgttt
     8881 ttgcgactca aaatggcatg attaagaaaa gtacagtgcc tctatttaaa acaacgcgtt
     8941 ttaataaacc tttaattgct actaaagtta aagaaaatga tgatttgatt agtgttatgc
     9001 gctttgaaaa agatcaatta attaccatca ttacaaataa aggtatgtct ttaacttata
     9061 atacaagtga actatcagat accggattaa gggcagctgg tgttaaatca ataaatctta
     9121 aagcagaaga tttcgttgtt atgacagaag gtgtttctga aaatgatact atattgatgg
     9181 ccacacaacg cggctcgtta aaacgtatta gttttaaaat cttacaagtt gctaaaagag
     9241 cacaacgtgg aataacttta ttaaaagaat taaagaaaaa tccacatcgt atagtagctg
     9301 cacatgtagt gacaggtgaa catagtcaat atacattata ttcaaaatca aacgaagaac
     9361 atggtttaat taatgatatt cataaatctg aacaatatac aaatggctca ttcattgtag
     9421 ataccgatga ttttggtgaa gtaatagaca tgtatattag ctaaaaacta tatgcaataa
     9481 cgaaattaaa tgataaaata cagtaatgtt aaattttgac taaattcaag ggatttatat
     9541 taaatgctga ccaagtactt atcgttaaaa tagcgatata ctttgaccaa actaatataa
     9601 atctcttgta ttgtataaga aatgatgatg tatctctata tttactgaag tttcaattcg
     9661 gtctaatttt atagaaggtg catgattcat gtagaggatg cgaggggttt attttgaaag
     9721 atttcgatag tttaattcct ggatggttta aagaatttgt ccatgttggt accgatttaa
     9781 tatggtctca atatttaatt ggtctattat tgacagctgg attcttcttt acaattagtt
     9841 ctaaattcgt ccaattacga atgttacctg aaatgtttag agctttagta gaacgtccag
     9901 aaactttaga agatggtaag aagggtattt cgccattcca agcatttgcg attagtgctg
     9961 gttcgagagt tggtactggt aatattgctg gtgttgcgac tgcgattgtt ttaggtggtc
    10021 caggtgcagt attttggatg tgggttattg catttatagg tgcagcgagt gcatttatag
    10081 aagcgacttt ggctcaggtt tataaagtac atgataaaga tggtggattc cgtggtggtc
    10141 cagcttacta tattactaaa ggtttaaatc aaaaatggct aggtatcgta tttgcgattt
    10201 taattacaat tacatttgca tttgtattta acacagtgca atctaataca attgcggagt
    10261 cgttaaatac gcaatataat attagtccag taatcacagg tattatttta gcaatcgtaa
    10321 ctgctattat tatatttggt ggtgtacgta gtattgctac gttatcttcg ttaattgtac
    10381 cgattatggc tattatttac attggtatgg ttttagtaat attgctattt aatttagatc
    10441 aaattgttcc tatgataggt acgattatta aaagtgcatt tggtatcgaa caagtaactg
    10501 gtggcgctgt aggtgctgcg gttcttcaag gtattaaacg tggtttattc tctaatgaag
    10561 ctggtatggg ttctgcgccg aatgcagcgg caactgctgc agtgccacac ccggtcaagc
    10621 aaggtttaat ccaatcatta ggtgtgttct ttgatacaat gttggtttgt acagcaactg
    10681 caatcatgat tttattatat tcaggactga aatttggtga taacgcacct caaggtgttg
    10741 cagttaccca atcagcactt aatgaacatt taggttctgc tggaggtatt ttcttaacaa
    10801 tagcagttac attattcgcc ttttcatctg tggtaggtaa ttactattac ggtcaatcta
    10861 atattgaatt tttatcaaca aaccgtgtga tattatttat ttttagatgt tttgttgtag
    10921 tacttgtctt tgtcggtgca gttgtaaaaa cagaaacagt atggaataca gcagacttat
    10981 ttatgggctt aatggcaatt gtaaacatta tttccattat aggactgtcc aatgtagctt
    11041 ttgcattgat gaaagattat caaaagcaga aaaaagaagg caagaatcct gtttttaaac
    11101 ctgaaaactt agaaattaac ttatttggaa ttagtgcttg gggcgctaac aaatataaga
    11161 actctgataa ataaatattt ttaatgtaac gattcatttt ataaaaatca aataaaccct
    11221 gaggttttca aattaatgcg aaaatctcag ggttttaaat ttgtactcaa taattatctg
    11281 tatcattaca ctagtt
//
LOCUS       DAKSQE010000085        11055 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.085, whole genome shotgun sequence.
ACCESSION   DAKSQE010000085 DAKSQE010000000
VERSION     DAKSQE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11055
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.085"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            378..1217
                     /locus_tag="O0V33_001955"
     CDS             378..1217
                     /locus_tag="O0V33_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCX8976579.1"
                     /translation="MKKSLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKTVKSPVRKASQAKKVVQQPVQQASKKVVVKQAPKQTVAKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            1391..2041
                     /locus_tag="O0V33_001956"
     CDS             1391..2041
                     /locus_tag="O0V33_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCX8976580.1"
                     /translation="MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYL
                     ATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVET
                     FIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFE
                     IYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            2038..2274
                     /locus_tag="O0V33_001957"
     CDS             2038..2274
                     /locus_tag="O0V33_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HCX8976581.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            2543..4528
                     /gene="uvrB"
                     /locus_tag="O0V33_001958"
     CDS             2543..4528
                     /gene="uvrB"
                     /locus_tag="O0V33_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCX8976582.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIKEIVEGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            4536..7382
                     /gene="uvrA"
                     /locus_tag="O0V33_001959"
     CDS             4536..7382
                     /gene="uvrA"
                     /locus_tag="O0V33_001959"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCX8976583.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNINLSE
                     QDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIEVPEYRRPASDRKISIRGARSNNLKG
                     VDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            7993..8925
                     /locus_tag="O0V33_001960"
     CDS             7993..8925
                     /locus_tag="O0V33_001960"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCX8976584.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            8931..9773
                     /locus_tag="O0V33_001961"
     CDS             8931..9773
                     /locus_tag="O0V33_001961"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCX8976585.1"
                     /translation="MGGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALV
                     KAGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYAENPGEIIKIWHGGIAIHGGLI
                     GGFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGPVSRAFLEQL
                     HLPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFFLYLTWYSIG
                     RFFIEGLRTDSLMLTSNIRVAQLVSILLILISISLIVYRRIKYNPPLYSKVGALPWPT
                     RKVK"
     gene            9781..10266
                     /locus_tag="O0V33_001962"
     CDS             9781..10266
                     /locus_tag="O0V33_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCX8976586.1"
                     /translation="MRKFLSKEYHRTNPLWHVYRLVKFSKVFKNVIIIEFSKFIPSMV
                     LKRHMYKQILNINIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALVDEF
                     RYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIR
                     R"
     gene            10274..>11055
                     /locus_tag="O0V33_001963"
     CDS             10274..>11055
                     /locus_tag="O0V33_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCX8976587.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMANQKFKQREYKRAAEYFEKVLEL
                     SPDDLEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLKEDSTDIYALCHYTL
                     LLYNTKENEQYQKYLKILNKVVPMNDDESFKLGIVLSYLKQYR"
BASE COUNT         3998 a         1522 c         2158 g         3377 t
ORIGIN      
        1 gctctttggt ttttttaaag gtagctaaat aaattgtaaa ttagattttg gaatatgatt
       61 tgtttatgaa tatttaagta caattcggtg gtttttgacg aaatattatc ctttataaaa
      121 gtttcgtttg ttaaacaaat ctatgtactg tgcattaagt aatattacct tcaacgaatt
      181 tcaatgtatt gaattaaatg ttgaaaacgt tgtcaatcat ttgcagtgaa tacattttac
      241 accgagtagg tagtttgcat tcttttttat attgttaacc atttgattac atcgttataa
      301 caatagcttt tgacaaaatg tattgtgcta tagtatttgc atacttaaaa tactaacagc
      361 aaaggaatga cagcaagatg aaaaaatctc ttacagtgac ggtttcgtca gtgttagctt
      421 ttttagcttt aaataatgca gcacatgcac aacaacatgg cacacaagta aaaacacctg
      481 ttcaacataa ttatgtatca aatgttcaag cacaaacgca atcaccgaca acttatacag
      541 tagttgctgg cgattcatta tataagattg ctttagagca tcacttaacg ttgaatcaat
      601 tatattcata caatcctggt gtaacacctt taatttttcc tggtgacgtg atttcacttg
      661 tgcctcaaaa taaagtgaaa caaactaaaa cggttaaatc accagtaaga aaagcaagcc
      721 aagctaaaaa ggtagtacaa caacctgtac aacaagcgtc taaaaaagta gtagttaagc
      781 aagcacctaa gcaaacagta gctaagacag ttaatgtagc atacaaacct gctcaagtac
      841 aaaaatcagt accaactgta cctgttgcac ataactacaa taaatcagtt gctaacagag
      901 gaaacttata tgcttatgga aactgcacat attatgcttt cgatcgtcgt gcacaattag
      961 gtagaagtat aggaagttta tggggcaatg caaataactg gaattacgca gcaaaagttg
     1021 caggatttaa agtagataaa acaccagaag ttggcgctat tttccaaaca gctgctggac
     1081 catatggaca tgttggtgtt gttgaatctg taaatcctaa tggaacaatt actgtttcgg
     1141 aaatgaacta tgctggattt aatgttaaat cttcaagaac aattttaaat ccaggaaaat
     1201 ataattacat ccactaagta acatatcaag acaagactat cctcttaacc tgtttaagta
     1261 acaggttgag aggatttttt ggtatcatct aatcagactt atataaagaa gatatttaaa
     1321 tgatatttaa ttgaatgata tgtaaaaaga aagtataatt tatgattaat taatggagga
     1381 ggtaattgaa atgggtgtac atcaatattt taaaagatta tcagatatgg aaagacttat
     1441 aagattacct ggaaaattta aatattttga acacaatgtt gcagcgcact cctttaaagt
     1501 aactaaaatt gctcaatatc tagcaacagt tgaagaatat catggacgaa agattaattg
     1561 gaaaagctta tatgaaaaag cattaaatca tgatttcgct gaagtgttta ctggtgatat
     1621 aaaaacacct gttaaatatg cgagtagtga attaaaaaaa ttattttcgc aagttgaaga
     1681 agaaatggta gagaccttta ttgaagaaga aattccatta caatatagag atgtttataa
     1741 gcaacgactg caagaaggta aagatgattc attagaaggc caaatacttt cagttgctga
     1801 taaaattgat ttgctttatg aaacatttgg agaaatacaa aaacgtaatc ccgaagaatt
     1861 atttttcgaa atttatgaaa tgagtctaga aacaattatt caatttgacc atttagcatc
     1921 tgtacaagat tttattaata atatcattcc agaaatgttg actgaaaact ttatacctag
     1981 aacagaatta agagaaacaa ctatgaacat tttaaataaa agaaaagagg aaaatgaatg
     2041 atatggtatt ttagcgcagc attctttcca tgtgtcttgg tagtattgtt tagtgtaata
     2101 acaagaagta aatgggtcgg tactattctg acattaattt taattggtgc ctcaatctat
     2161 aaagagtatt tccataacga gtggattatt tttattgatg tggtgtcatt attagctggt
     2221 tatttaatta tagatcaact cgaatttcat aaacatcaag atgaagatcg ctaagattaa
     2281 ctttaaaata atgtttcaaa caaatttgtt gaaacaaaat gatgattaat ataatgtgta
     2341 tttacatact aaaaataaca agataaacga tttgatttaa ggcaagcata gttagcacaa
     2401 ctatgtttgt ttttctttgt tcgacatttt tacgaacaaa cgtttgcttt ttgtgtgact
     2461 actttgctaa aatatgtaat gagaaagcga agagttgagc ggaaatataa ataatacgtt
     2521 gaaagaggag gcatatgtga ctatggttga acattatcct tttaaaatac attctgattt
     2581 tgagcctcaa ggtgaccaac cgcaagcaat taaggaaatc gtggaaggta ttaaagcggg
     2641 gaaaagacat caaactttat taggtgcaac tggcacaggg aaaacattta cgatgagtaa
     2701 tgttattaaa gaagttggga aaccaacgtt aattatcgcg cataacaaaa cattagcagg
     2761 acaattatat agtgagttta aagaattttt tcctgaaaac agggtggaat actttgtaag
     2821 ttactatgat tattatcaac ctgaggcata cgtaccgtct actgacactt ttattgaaaa
     2881 agatgcctca atcaatgatg aaattgatca actacgacat tctgctacaa gtgcattatt
     2941 tgaacgcgat gatgtaatta ttattgctag tgtaagttgt atatatggtt taggtaatcc
     3001 tgaagaatat aaagatttag tagtaagtgt tcgagttggt atggaaatgg atagaagtga
     3061 attacttaga aaacttgtag atgtgcaata tacacgaaat gacatcgatt tccaacgagg
     3121 aacgtttcga gtgcgtggtg atgttgttga aatattccca gcctctaaag aagaactttg
     3181 tataagggtt gagtttttcg gcgatgagat tgaccgtatt cgagaagtta actacctaac
     3241 tggtgaagtg ttgaaagaaa gagaacattt tgcgatattc ccagcttctc acttcgtaac
     3301 acgtgaagaa aagttgaaag ttgcgattga acgtattgaa aaagaattgg aagaacgatt
     3361 gaaagaatta cgagatgaga ataaattact agaagcgcaa aggttagaac agcgtaccaa
     3421 ctatgattta gaaatgatgc gagagatggg attctgttca ggaattgaaa actattccgt
     3481 acatttaact ttgcgaccac tgggttcgac accatatact ttattggatt actttggcga
     3541 tgattggtta gtaatgattg atgaatcaca tgtgacatta ccgcaagttc gaggcatgta
     3601 taacggagac agagcgcgta aacaagtttt ggtggatcat ggatttagat taccgagtgc
     3661 attagataac cgtcctctta aatttgaaga atttgaagaa aagacaaaac aacttgtgta
     3721 tgtatctgca acgcctggac catacgaaat tgaacatacg gataagatgg ttgaacaaat
     3781 tattcgtcct actggtttac tggatcctaa gattgaggtt agacctactg aaaatcaaat
     3841 tgacgattta ttaagtgaaa ttcaaacaag ggttgagcgt aatgaacgcg tacttgttac
     3901 aacgctcact aaaaagatga gtgaagattt aaccacatac atgaaagaag cgggtattaa
     3961 agttaattat ctgcattcag aaatcaagac attagaacga atcgaaataa ttagagactt
     4021 acgaatgggt acatatgatg ttatcgtagg tattaattta ttaagagagg gtattgatat
     4081 accagaagtt tctctagttg tcatattaga tgcagataaa gaaggatttt tacgttctaa
     4141 ccgctcatta attcaaacaa taggtagagc tgcgcgtaac gataaaggtg aagtcattat
     4201 gtatgccgat aaaatgactg attcgatgaa gtatgcaatt gatgagacac aacgtcgtcg
     4261 agaaatacag atgaaacata atgaaaaaca tggtattaca cctaaaacaa ttaataaaaa
     4321 aatacatgat ttaattagtg ctactgttga aaatgacgaa aataatgaca aagcacaaac
     4381 tgtgatacct aagaagatga cgaaaaaaga acgtcaaaag acaatcgaca atatagaaaa
     4441 agaaatgaaa caagcagcga aagatttaga tttcgagaaa gctacagaat taagagatat
     4501 gttatttgaa ttaaaagcag aagggtgaca agtaaatgaa agaaccatcc atagtagtaa
     4561 aaggtgctcg tgcgcataac ttgaaagata ttgatatcga actacctaaa aataaattaa
     4621 ttgttatgac aggattatct gggtcaggta aatcgtcatt agcattcgat actatatatg
     4681 ctgaaggaca acgacgttat gttgaatcat taagtgccta tgcgcgtcaa tttttagggc
     4741 aaatggacaa accagatgtt gatactattg aaggattatc gccagcaatt tcaatagatc
     4801 aaaaaacaac aagtaaaaat ccaaggtcaa ctgttgcaac agtaacagaa atatatgatt
     4861 atatacgttt gttatatgca cgtgttggta aaccttactg tccaaatcac aatatagaaa
     4921 ttgaatcgca aacagtacaa caaatggttg accgcattat ggaattagag gcacgtacaa
     4981 agattcaatt attagcacct gtcatcgctc atcgtaaagg tagtcatgaa aagctaatcg
     5041 aagatattgg taaaaaaggt tatgtacgtt taagaatcga tggcgaaatt gttgatgtaa
     5101 atgatgtacc tacattagat aagaacaaga atcatacaat agaagttgtt gtagaccgat
     5161 tagttgttaa agatggaatt gaaacacgac tagctgactc tatagaaact gcgttagagc
     5221 tttcagaagg acaattaaca gtcgatgtca ttgacgggga agaccttaag ttttcagaaa
     5281 gccacgcttg tcctatatgt ggattttcaa tcggagaatt agaaccaaga atgtttagct
     5341 ttaacagtcc ttttggtgct tgtccaacat gtgatggctt aggtcaaaag ttaacagtcg
     5401 atgtagactt ggttgttccc gacaaagata agacgctaaa cgaaggtgca atagaaccat
     5461 ggataccgac gagttctgat ttttatccaa cattgttaaa acgtgtttgt gaagtttata
     5521 aaatcaatat ggataaacct tttaaaaagt taacagaacg tcaacgtgat attttattgt
     5581 atggttcggg tgacaaagaa attgaattta catttacaca acgtcagggt ggtactagaa
     5641 aacgaacaat ggttttcgag ggtgtagttc ctaatataag tagacgattc catgaatctc
     5701 cttcagaata tacacgtgaa atgatgagta aatatatgac tgaactacct tgcgaaactt
     5761 gtcatggaaa gcgattgagt cgtgaagcgt tatctgttta tgtaggtggt ttaaatattg
     5821 gtgaagtagt cgaatactca attagtcaag cgctgaacta ttataaaaac attaatttgt
     5881 cagaacaaga tcaagcgatt gcaaatcaaa tattgaaaga aattatttcc cgactcactt
     5941 ttttaaataa tgtgggactt gaatatttaa cgctaaacag agcttcaggt acactttcag
     6001 gtggtgaagc acaacgtatt cgattggcaa cgcaaattgg gtcgcgtttg actggtgtct
     6061 tatatgtatt agatgagcca tcaattggac tgcatcaaag agataatgat cgattaatta
     6121 atacacttaa agaaatgaga gatttaggaa atactttaat tgtagttgaa cacgatgatg
     6181 atacaatgcg tgcggctgat tacttagtgg acatagggcc tggtgctggt gagcatggag
     6241 gacagattgt gtctagtggt acacctcaaa aggtaatgaa agataaaaaa tctttaacag
     6301 gacaatactt gagtggtaag aaacgtattg aagtacctga atatcgcaga ccggcttcag
     6361 atcgtaaaat ttctatacgt ggagctagaa gtaacaacct taaaggggtt gatgtggaca
     6421 taccactttc aatcatgacg gtcgttacag gtgtatcagg ttctggtaaa agctcattag
     6481 taaatgaagt attatacaaa tcattagctc aaaaaatcaa taaatctaaa gtaaagccag
     6541 gattgtacga taaaattgaa ggtattgatc aacttgataa aattattgat attgatcaat
     6601 caccgatagg tagaacgcca cgctctaatc cagcaacata tactggcgtg tttgatgata
     6661 tacgtgatgt gtttgcgcaa acaaatgaag ctaaaattcg aggatatcaa aaaggtcgtt
     6721 ttagttttaa tgtaaaaggt ggacgctgtg aagcttgtaa aggtgacggt attattaaaa
     6781 ttgaaatgca ttttttacct gatgtttatg ttccttgtga agtgtgtgat ggtaaacgat
     6841 ataatcgcga gacactagag gttacttaca aaggtaaaaa tattgctgac attttagaaa
     6901 tgactgttga agaagcaaca caattttttg aaaatattcc taagattaag cgcaagctac
     6961 aaacactagt tgatgttggt cttggatacg tcacattagg tcaacaagct acaacattat
     7021 caggtggtga ggctcaacgt gtgaaacttg catctgaact tcataaacgt tcaactggta
     7081 aatctattta tatcctagac gaaccgacaa cagggttaca tgttgacgat attagtagat
     7141 tattaaaagt attaaaccga ttagttgaaa atggtgacac tgttgtaatt attgaacata
     7201 acctagatgt tatcaaaaca gcagactata ttatagactt aggtcctgaa ggtggtagtg
     7261 gcggtggtac tattgttgcg actggaacac ccgaagatat tgctcagaca aagtcatcat
     7321 atacaggaaa gtatttaaaa gaagtacttg aacgagataa acaaaatact gaagataaat
     7381 aagattaaaa gaagtgaagg atgttgtaaa tttatccttc acttcttttt attaatttag
     7441 taatgaatag tagaaagaaa agatgcgtaa aaagaattat gttaagataa ggtcaatcta
     7501 gagtagctaa acataaatcg aaactgggag cgggacagaa atgataaaga atcactaatg
     7561 atttattatg tagtggttct ttgtcattag ccacagctat tgtgtactta aaaataggaa
     7621 tgcatgagtg caactcatgc ataagaaata ctaatttcta aagaaaaagt atctctttat
     7681 gttgggggcc cgccccaact tgcattgtct gtcgaaattg agaatccaat ttctctgtgt
     7741 tggggccccg ccaactaatt cgaatttatc attttagagc ttaggtcatt gatttatggc
     7801 tcggactttt atggcgatat gaaacatgta aattaagcaa gcaataaatt aatgattgat
     7861 attgacttgt aaaataataa caataatgaa caattaatat ttattttagc ttttcaatgt
     7921 agattggtgt tatatttttg atatgataag aagagatgta agagtaggga taaatacaat
     7981 tgaggtgaac ccatgttaac gacagaaaaa ctagttgaaa cattaaagtt agatttaatc
     8041 gctggtgaag aagggctatc gaagccaatt aaaaatgctg atatatcaag accgggctta
     8101 gagatggcag gttatttttc acattatgcg tcagatagaa tacaactatt aggaacaacg
     8161 gaactatcgt tttacaattt attaccagat aaggatcgcg caggtcgtat gcgtaaacta
     8221 tgcagaccag aaacgcctgc aattattgtg acacgtggat tgcagccacc agaagaatta
     8281 gttgaagctg caaaagaatt aaatacgcca cttatagttg ctaaagatgc cactacaagt
     8341 ttaatgagtc gcttaacgac gtttttagag catgcacttg caaagacgac atctttacat
     8401 ggagttttag tagatgttta cggtgttggt gtactaatta ccggtgattc aggaataggt
     8461 aaaagtgaga ctgcgttaga attagtgaag cgtgggcata gattagtagc agatgataat
     8521 gtagaaatac gtcaaattaa taaagatgaa ctaataggga aaccaccaaa gttaatagaa
     8581 catctattag aaatacgtgg actaggtatt atcaatgtta tgactttatt tggcgcgggt
     8641 tcaatattaa ctgaaaaacg aattagatta aatattaatt tggaaaactg gaacaagcaa
     8701 aagttatatg accgcgtagg tcttaatgaa gagacgttaa gtattttaga tactgaaata
     8761 actaaaaaaa caatacctgt aagacctggt agaaatgttg cggtaattat tgaggtcgct
     8821 gcaatgaact atcgattaaa tatcatgggc attaacactg ccgaagaatt tagtgaaaga
     8881 ttaaatgaag aaattatcaa gaacagtcat aagagtgagg agtaggttga atgggtggta
     8941 ttgtatttaa ctatatagat cctgtggcat ttaacttagg accactaagt gtacgatggt
     9001 atggaattat cattgctgtc ggaatactac ttggttactt tgttgcacaa cgtgcactag
     9061 ttaaagcagg attacataaa gatactttag tagatattat tttttatagt gcactatttg
     9121 gatttatcgc ggcacgaatc tattttgtaa ttttccaatg gccatattac gcggaaaatc
     9181 caggtgaaat tattaaaata tggcatggtg gtatagcaat acatggcggt ttaataggtg
     9241 gctttattgc tggtgttatt gtatgtaaag tgaaaaattt aaacccattt caaattggtg
     9301 atatcgttgc gccaagtata attttagcgc aaggaattgg acgctggggt aactttatga
     9361 atcacgaggc acatggtgga cctgtgtcac gcgctttttt agaacaatta catttgccta
     9421 attttataat agaaaatatg tatattaacg gccaatatta tcatccaaca ttcttatatg
     9481 aatccatttg ggatgtcgct ggatttatta tcttagttaa tattcgtaaa catttaaaat
     9541 taggagaaac attcttttta tatttaactt ggtattcaat tggtcgattc tttatagaag
     9601 gattacgtac agatagctta atgctcacaa gtaatattag agttgcacaa ttagtatcaa
     9661 ttcttttaat tttaataagt ataagtttaa ttgtatatag aaggattaag tataatccac
     9721 cgttgtatag caaagttggg gcgcttccat ggccaacaag aaaagtgaag tagtgatatt
     9781 ttgagaaaat ttttatcaaa agaatatcat cgtacaaacc ctttatggca tgtataccgt
     9841 cttgttaaat tttcgaaagt ttttaagaat gtaatcatca ttgaattttc gaaatttatt
     9901 ccaagtatgg tactgaaaag acatatgtat aaacaaattt taaatattaa tatcggtaat
     9961 caatcgtcga tagcttataa agtaatgtta gatatttttt atccagaact gattacgatt
    10021 ggtagtaaca gtgttattgg ttacaatgta acaattttga cgcatgaagc attagttgat
    10081 gaatttcgtt atggaccagt gacgatagga tctaacactt tgattggtgc aaatgctacc
    10141 attttacccg gtataacgat tggtgacaat gtaaaagttg cagctggtac ggttgtttca
    10201 aaagatatac cggataatgg atttgcatat ggcaacccta tgtatataaa aatgattagg
    10261 aggtgacaat tttatggcgc aaaagaataa taatgtaatt ccaatgactt ttgatgatgc
    10321 attttatcgt aaaatggcta atcagaagtt taaacaaaga gaatataaac gagctgctga
    10381 atactttgaa aaagtgttag aattgtcacc tgatgatctg gaaattcaaa ttgattatgc
    10441 acaatgtcta gtgcaacttg gtattgctaa aaaagcagaa catttatttt atgacaatat
    10501 tatttataat aggcatctag aagatagctt ttatgaattg agtcagctca acattgaagt
    10561 taacgaacca aacaaggcat tcttgtttgg tattaattat gttattgtta gcgacgacca
    10621 agattataga gatgaattag atcaaatgtt tgatgtgaaa tatcaaagtg aagaacaaat
    10681 tgaacttgaa gctcaattgt ttgtagttca aatactattc caatatcttt tttctcaagg
    10741 tcgattaaaa gatgcaaaga attatgtctt acatcaacca caagaagttc aagatcatcg
    10801 tgtcgtacgt aatttactgg caatgtgtta tttatatctc ggcgaatatg atacggctaa
    10861 agcattgtac gaagcactat taaaagagga tagtacagat atatatgcat tatgccatta
    10921 tactttgcta ctttataaca ctaaggaaaa tgaacaatac caaaagtatt taaaaatatt
    10981 aaataaagtt gtacctatga atgacgatga aagttttaaa ttaggtattg ttttaagtta
    11041 tttaaagcag tatcg
//
LOCUS       DAKSQE010000086        10575 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.086, whole genome shotgun sequence.
ACCESSION   DAKSQE010000086 DAKSQE010000000
VERSION     DAKSQE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10575
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.086"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..386
                     /locus_tag="O0V33_001964"
     CDS             <1..386
                     /locus_tag="O0V33_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020807974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HCX8976588.1"
                     /translation="AINKTQNNGYENEYFYIVANIPTLQEYRKYYEPLIKKNNLNFKK
                     GMKEARKGVGYKAEIEVHITLFSKSSNFSKDKKLDDVIDLSESTKKLHLNFENTKIFL
                     QLAKSTISTNRVNYSDNESIRIEVE"
     gene            444..1352
                     /locus_tag="O0V33_001965"
     CDS             444..1352
                     /locus_tag="O0V33_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCX8976589.1"
                     /translation="MFKRTKLILIATLLLSGCSTTNNESNNETKSVPEEMEASKYVGQ
                     GFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGDGVEVFVHCDDH
                     DIVFNASIPFDKSIIDSNSSLRSEDKGDDMSTLVGTVLSGFEYRAQKEKYDNLYKFFK
                     DNEKKYQYTGFTKEAINKTQNSGYENEYFYITTIPYNLTEYRDYFEPLLNKSDSEFSK
                     ELSNVKKQLKDKSKLSVTTTLFSKKKNYTKKSNSENVIKMAEEIKKEKEIPNGIDLSI
                     KFSDNKINTVKPNYNGKSTSEYGVFD"
     gene            1557..1721
                     /locus_tag="O0V33_001966"
     CDS             1557..1721
                     /locus_tag="O0V33_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976590.1"
                     /translation="MTILTQLSDRIKKTIPTTDKTTNQSNLPNQSTQITNQNYPNNAR
                     KSPMKHSPIT"
     regulatory      1804..1938
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2000..2545
                     /locus_tag="O0V33_001967"
     CDS             2000..2545
                     /locus_tag="O0V33_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCX8976591.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIIVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     IVISIVFILLYRRLANFLKRI"
     gene            complement(2651..2899)
                     /locus_tag="O0V33_001968"
     CDS             complement(2651..2899)
                     /locus_tag="O0V33_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCX8976592.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            3007..3960
                     /locus_tag="O0V33_001969"
     CDS             3007..3960
                     /locus_tag="O0V33_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCX8976593.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLENITEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTM
                     QQDFRFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEFEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTRLLEQFTIEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVEQEDQLQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            3950..5329
                     /locus_tag="O0V33_001970"
     CDS             3950..5329
                     /locus_tag="O0V33_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCX8976594.1"
                     /translation="MLHDILRNKFGFENFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLR
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLEIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQDVLTTLHSFYSIGALKQVFKKSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIAILNKKKVIRSIGFD
                     EKLQNLFLR"
     gene            5482..6942
                     /gene="ebpS"
                     /locus_tag="O0V33_001971"
     CDS             5482..6942
                     /gene="ebpS"
                     /locus_tag="O0V33_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HCX8976595.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEGYYNKNAFAMDKSHPEPIEDNDKHETIKEAENNTEHSTVSDKSEA
                     EQSQQPKPYFATGANQANTSKDKHDDVTVKQDKDESKDHHSGKKGAAIGAGTAGVAGA
                     AGAMGVSKAKKHSNDAQNKSNSGKVNNSTEDKASEDKSKEHHNGKKGAAIGAGTAGLA
                     GGAASNSASAASKPHASNNASQNNDEHDHHDRDKERKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKDDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRI
                     AIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            7080..7166
                     /locus_tag="O0V33_001972"
     CDS             7080..7166
                     /locus_tag="O0V33_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976596.1"
                     /translation="MAYQLNFLHSHQKDKTPQSTNLTRKNKK"
     gene            7327..8313
                     /locus_tag="O0V33_001973"
     CDS             7327..8313
                     /locus_tag="O0V33_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HCX8976597.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES"
     gene            complement(8428..9396)
                     /locus_tag="O0V33_001974"
     CDS             complement(8428..9396)
                     /locus_tag="O0V33_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCX8976598.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDINPISLHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNEKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNMPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            9473..10132
                     /locus_tag="O0V33_001975"
     CDS             9473..10132
                     /locus_tag="O0V33_001975"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCX8976599.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKTIEEVTDEILAMVSQI
                     K"
     gene            10163..10279
                     /locus_tag="O0V33_001976"
     CDS             10163..10279
                     /locus_tag="O0V33_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976600.1"
                     /translation="MQRLMHIQIECADNDLLNLKGDFYSTMNESCLFTFGKS"
     gene            10396..>10575
                     /locus_tag="O0V33_001977"
     CDS             10396..>10575
                     /locus_tag="O0V33_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976601.1"
                     /translation="MSVTNLRIAKEADLVTKNVLCNNSRKMHYIRSSYYSNVKVAYLR
                     GFLFYKSIKFYYNISC"
BASE COUNT         4008 a         1600 c         1804 g         3163 t
ORIGIN      
        1 aggcaattaa caagacacaa aataatggct atgaaaatga atatttttat atagttgcaa
       61 atataccgac gctccaagaa tatagaaaat attacgaacc cctaataaag aagaataatc
      121 tgaattttaa aaaaggtatg aaagaagcaa ggaaaggagt aggctataaa gctgaaatag
      181 aagtacatat tacattgttt tcgaaaagta gtaacttttc aaaggacaaa aaattagatg
      241 atgttataga tttgtctgaa agtacgaaaa agttacacct taattttgaa aatacgaaaa
      301 tatttttaca actagcaaaa tctactatta gtactaatcg agttaattac agtgataatg
      361 agtctataag gattgaggtc gaatgacttg aaatcagcta atttctctat attctaaaca
      421 aacacataaa tgggtgatga gctatgttta aaagaactaa actaatctta atagcaacac
      481 tactgctatc aggatgttca actaccaata acgaatccaa caatgaaaca aaatcagtac
      541 cagaagaaat ggaagcttcc aaatatgtag gacaaggctt tcaaccacct gcagaaaaag
      601 atgcgattga atttgcgaag aagcataaag ataaaatagc taaacgtggt gaacaatttt
      661 ttatggataa cttcggtcta aaagttaaag ctaccaatgt tgtaggtagt ggcgacggtg
      721 tagaagtatt cgtgcattgt gatgaccacg acatcgtatt taatgcgagt attccatttg
      781 acaaatcaat tattgacagt aatagctcat taagaagtga ggacaaaggc gatgatatga
      841 gtactttagt tggtacagtg ttgagtggct ttgaatatcg agcacaaaaa gaaaagtatg
      901 acaatttata taaatttttc aaagataatg aaaagaaata tcaatataca ggatttacaa
      961 aagaggcaat taacaagaca caaaatagtg gatatgaaaa tgaatatttt tatattacga
     1021 ctataccgta taatttaact gagtatagag actattttga accattatta aacaaaagtg
     1081 acagtgaatt ttcaaaagaa ttgtcaaatg ttaagaagca attaaaagat aagtctaaac
     1141 tttcggtaac tactactcta tttagtaaaa aaaagaacta tactaaaaaa agtaacagtg
     1201 aaaatgtaat aaaaatggca gaagaaataa aaaaagaaaa agagatacca aacggtatag
     1261 atcttagtat aaaattttcg gacaataaaa taaatacagt taaaccaaat tataacggta
     1321 aaagcacttc agaatatggt gtgtttgact aagaataaaa taaatgatga atatttagcg
     1381 gagattagtg tatattggct agattaagta ttgaaagaca attctactat tgttgtgaag
     1441 gcaaataagt agaaggtttt aagtataact tctggcgatt taaataataa tatatatgga
     1501 agtgctgata caaaactttt taacctacta gattcttata atttgctttc cattttatga
     1561 cgattttgac ccaattgagt gatagaatca aaaaaaccat cccaacaaca gacaaaacca
     1621 caaaccaatc aaacctaccc aatcaaagta cgcaaatcac caatcaaaac tacccaaaca
     1681 atgctcgcaa atcaccaatg aaacattctc caattacgta actatgatct aattaaagca
     1741 tagttattga ggttttgtga tatatagtat aaaattaatg agaattaaat ttaataatgt
     1801 aaaattcatc ttcggggtcg ggtgtaattc ccaaccggca gtaaataaag cctgcgacct
     1861 gctagtatgt atcatattag tggctgatct agtgagattc tagagccgac agtatagtct
     1921 ggatgggaga agatggaggt tttttgttgt gcaataatcc tcctattctt acgagatgaa
     1981 tggaaggaga aaattgaata tgcaacaaaa taaacgtctt atcacaataa gtatgttgag
     2041 cgcgattgcg tttgtattaa cttttatcaa gtttcctata ccatttttgc caccatactt
     2101 aactttagat tttagtgatg taccgtcact actagctaca tttacgtttg gaccagttgc
     2161 cggtatcata gttgcactgg ttaaaaattt attgaactac ttatttagta tgggcgatcc
     2221 agttggacca tttgctaact ttttagcagg cgcaagtttc ttattaactg cttacgccat
     2281 ctataaaaat aaacgttcaa caaaatcttt gattactgga ttaatcattg caacaatcgt
     2341 tatgactatt gtgttgagta tcttgaacta tttcgttcta ttacctttat acggtatgat
     2401 atttaactta gctgatatcg caaataatct taaagtaatc attgtttcag gaattatacc
     2461 attcaatatt attaaaggta tcgtcatttc tattgtattt attttactat atagaaggct
     2521 tgcgaatttc ttgaaaagaa tttaatcaaa ttaaagcaaa ataatataca cataataata
     2581 aaaagcaggt gactatcaat aaacgatagc ttgcctgctt tttctatgaa acatttgtct
     2641 aaaaaatcaa ttattcaaat tttaaagcgt ccccatcaaa tgattcgtct gcaattttaa
     2701 tagaatctgt agggcatcca tcaattgcat cttccatatc ttcatataat tcctcaggta
     2761 cttctgcggt accttggtta tcgtcaagga ttacgaaagc aataccttcg tcgtcgtaat
     2821 catatatatc tggcgctgct gcaccgcatg caccacatgc aatacaagta tccatatcaa
     2881 cgattgtata ttttgccaat gtcttcgcct cctttgataa aaatgctaaa atagtaatgt
     2941 gactaaaatt ttagacagca tcatttttat tttcaaatta tccgttttac agagtgaggg
     3001 ttaaacttgc aacacattat aaaaacagca ttacaacaaa catttaacta taaaacaaat
     3061 aaaagtattt acaatatctt agttggtaag aaatctcacc aaaccttttt tgacgcttgt
     3121 agtcaacaac agttgtcatt atatcacagt ttaccactat taaaatatcc gtcttttgag
     3181 ctatttttag aaaatataac tgaatttaat gctgaaatgg aaatcatgtt gcatcctaga
     3241 tatacatttg aaagtatggg gcaaacattt caagcaattc aactattagt gcaaaccatg
     3301 tctaatacta tgcagcaaga ttttcgtttc gtaccaatct ctcaaaataa taagatacaa
     3361 gaaactgtaa aaattgtcta taactatatt aaagaaaacc aattacaaat tgactttgaa
     3421 aatgaactac ataacttgtt taaagcaata actttaaaag ggccatgtta cttacattat
     3481 tatttgcaag gctatgatga accaatgtat acgagacagc aagttagttt aatagaaaaa
     3541 ctatctcaac agcaattgtt tgaattcgaa atgaataatt tagtgacaat gatgtttgaa
     3601 ttagaaagtg gagaatatac tattttatca aaaataataa tgaaacctac attattaaat
     3661 caaacctata ttacttatac aagattgctt gaacaattca cgatagaaga tatagcggct
     3721 caacaacaag ttaaaatcaa tactatcgaa gatcatgtac ttgaaatctt aatcaaaggt
     3781 tatatgtcta attacgatga ttatgttgaa caagaagatc aactgcaatt tttgaatttt
     3841 tatcaacaac atcgtggtga acgattaaaa ttttacaaag aacaatttga cacgttatca
     3901 tattttcaat taaaagtatt aatcgttggt tttgaaagag gtgatctgaa tgttgcatga
     3961 tattttacga aacaaatttg gcttcgagaa ttttaaaccg ggacaacagg aaattataga
     4021 aagtataatg tctcaacaac acactctagg tattcttcca actggaagtg gaaagagttt
     4081 gtgttatcaa atacctacgt atttatcagg taagccgaca ttaattatct caccgttaat
     4141 atctttaatg gatgaccaag ttatgcagtt gaaaataaat ggagaaaaac gtgtaacatg
     4201 tattcactct ggtatggatg aaattgagaa aaagcataat attaaatgtt tacgacatag
     4261 ccgcttcatc tttctaagtc cagaatttct cctgcagccg tcaaatttta aattaatatc
     4321 tatgatagac tttggcatga ttgttctaga tgaagcacat tgtctatctg aatggggata
     4381 tgatttcaga ccacattatg ctctaattgg aaaagtaaca aagcatttta aagaagcggt
     4441 tgtcttagca ttgacagcaa ctgcaccacc gcatttgcaa gatgatttga cggaaatgct
     4501 agagattcaa ttcaacgtta ttaaaactac aatgaatcgc ccaaacataa gctttaagca
     4561 tcttaatttt catgatgatg aagataaaat tgagtggttg ctgccgtttc tacaacagtc
     4621 gggaccaacg attatttatg tctcatcgaa aaagatgtgt ctgaatttag cgcaacttat
     4681 ttatgattca ggttttctta ctggcattta tcatggtgat atgaattatc aagagcgaca
     4741 cacagttcaa caacaatttt taaataatga tattccgatt atagtcgcaa cgagtgcttt
     4801 tggtatggga attaataaaa aagatattcg cacaatcatt cactttcatc tttcaacaag
     4861 tccttctaac tacattcaag aaattggacg tgcgggtcgc gatggtgaac taagtcaggc
     4921 aattagttta tttcaaccgg acgataaata tattttagaa acgttattat ttgcagatat
     4981 gataacagaa gaagatgtac aaaatttcga aataggagaa tttttagcac ccgataaaca
     5041 agacgttttg acaacgttgc attcattcta tagtatcggc gccttgaaac aggtatttaa
     5101 gaaatcgttt aaacgaaagc aattaggatt ctttcgtatg attggctatt gcaaattgga
     5161 tcaatgtaga cggaagtatt tattagaatt tttcggtgaa tatccaccgg cacaagatcg
     5221 atgttgtgac aatgattcta atataactga tatcgcaatt ttaaataaga agaaggtaat
     5281 tagaagtatt ggatttgatg aaaagttgca aaatttattt ctcagatagt attactttac
     5341 taaaagaaaa ttgacaagct ataattagtg tatacacaat tgaaaaatga ttgaaataat
     5401 tttgaaaaat atacataaac atatgtcatg tgggtatatt ttatgtaaaa tcattgtaat
     5461 agaatagaaa ggaagatggc tatgtctaat aattttaaag atgactttga aaaaaatcgt
     5521 caatcgatag acacaaattc acatcaagac catacggaag atgttgaaaa agaccaatca
     5581 gaattagaac atcaggatac aatagagaat acggagcaac agtttccgcc aagaaatgcc
     5641 caaagaagaa aaagacgccg tgatttagca acgaatcata ataaacaagt tcacaatgaa
     5701 tcacaaacat ctgaagacaa tgttcaaaat gaggctggca caatagatga tcgtcaagtc
     5761 gaatcatcac acagtactga aagtcaagaa cctagccatc aagacagtac acctcaacat
     5821 gaagagggat attataataa gaatgctttt gcaatggata aatcacatcc agaaccaatc
     5881 gaagacaatg ataaacacga gactattaaa gaagcagaaa ataacactga gcattcaaca
     5941 gtttctgata agagtgaagc tgaacaatct cagcaaccta aaccatattt tgcaacaggt
     6001 gctaaccaag caaatacatc aaaagataaa catgatgatg taactgttaa gcaagacaaa
     6061 gatgaatcta aagatcatca tagtggtaaa aaaggcgcag caattggtgc tggaacagcg
     6121 ggtgttgcag gtgcagctgg tgcaatgggt gtttctaaag ctaagaaaca ttcaaatgac
     6181 gctcaaaaca aaagtaattc tggcaaggtg aataactcga ctgaggataa agcgtctgaa
     6241 gacaagtcaa aagaacatca taatggtaaa aaaggtgcag caatcggtgc tggaacagca
     6301 ggtttggctg gaggcgcagc aagtaatagt gcttctgccg cttcaaaacc acatgcctct
     6361 aataatgcaa gtcaaaacaa tgatgaacat gaccatcatg acagagataa agaacgtaaa
     6421 aaaggtggca tggccaaagt attgttacca ttaattgcag ctgtactaat tatcggtgca
     6481 ttagcgatat ttggaggcat ggcattaaac aatcataata atggtacaaa agaaaataaa
     6541 atcgcgaata caaataaaaa taatgctgat gaaagtaaag ataaagacac atctaaagac
     6601 gcttctaaag ataaatcaaa atctacagac agtgataaat caaaagatga tcaagacaaa
     6661 gcgactaaag atgaatctga taatgatcaa aacaacgcta atcaagcgaa caatcaagca
     6721 caaaataatc aaaatcaaca acaagctaat caaaatcaac aacagcaaca acaacgtcaa
     6781 ggtggtggcc aaagacatac agtgaatggt caagaaaact tataccgtat cgcaattcaa
     6841 tactacggtt caggttcacc ggaaaatgtt gaaaaaatta gacgtgccaa tggtttaagt
     6901 ggtaacaata ttagaaacgg tcaacaaatc gttattccat aatataacta tataaattgt
     6961 aactgaactg ctatatacga acgtgcatat tataaagttt ctctaaatat tattgaaaac
     7021 ttaaattgcg cttataagta tgtagcagtt tttttattta ataagtatgg acatgaatat
     7081 tggcatatca attaaatttt ttacatagtc atcaaaaaga taaaacacca caatcaacaa
     7141 atttaacgag gaagaataaa aaataaatca acatattaaa ttgtagtgtt attcaactcc
     7201 gtagctaaca attctctatg catattaaac aaattgtcga aaatatatca taaatcttca
     7261 agcacagact tagcgcatca atcactgaac tgttataata gtttgggatt aaaggaggcc
     7321 ggaacaatgc aaaaagttga aagtatcata attggtggag ggccatgcgg attaagtgcg
     7381 gctattgaac aaaaaagaaa aggtattgat accttaatta ttgaaaaggg taatgtcgtt
     7441 gaatcaatct acaattatcc tactcaccaa acatttttct catcaagtga taaattaagt
     7501 attggggacg taccgtttat tgttgaagaa agtaaaccaa gacgtaatca agcgctagtt
     7561 tattaccgag aagttgtaaa acatcatcaa ttaaaagtaa atgcatttga agaagtatta
     7621 actgttaaaa aaatgaataa taaatttact attactacga cgaaagatgt ttatgaatgt
     7681 cgatttttaa caatcgcgac aggctattat ggtcagcata atacattaga agttgaaggt
     7741 gcggatttac ctaaagtgtt ccattatttt aaagaggcac atccgtattt tgatcaagat
     7801 gttgttatta tcggtggtaa gaattcggct atcgatgctg ctttggagtt ggaaaaagct
     7861 ggtgctaacg tgacggttct atatcgtggt ggagattatt cgccttcaat taaaccgtgg
     7921 atacttccaa atttcacagc attagtaaat catgaaaaaa ttgacatgga atttaatgct
     7981 aatgttaccc aaataactga agatactgtg acttatgaag taaatggtga aagtaaaacg
     8041 atacacaatg attatgtatt tgcgatgatt ggttatcatc ccgattatga atttttaaaa
     8101 tctgtaggca ttcaaattaa tacaaatgaa tttggaacag cgcctatgta taataaagaa
     8161 acatacgaaa caaatatcga aaattgctac attgcaggtg tgattgctgc agggaacgat
     8221 gcgaatacca tttttattga aaatggtaaa ttccacggtg gtattattgc tcaaagcatg
     8281 ctagctaaga aacaaacgcc gttagaatca taaaaataaa ggtctatgta ctaaataact
     8341 tagttttaca acgactgaca ttcatgatat gtcaaatgag gttgatgact aattattgta
     8401 catagacctt tttatgttac gtaatcatta taattcaaaa tatgatttga tttcagcttt
     8461 atctaaatta ttgcttaacg cgactaataa ttttaatctt gcttttggac cattcaaacc
     8521 gttagaaaaa ataaaacctt gttgtgcgag ttggtaacca ccaccatcgt atgcgtaagt
     8581 tggactcaca ataccattaa aggaacgtga aactagcaca ataggtatat ttaaagatac
     8641 taattgttga atgccttcta atgcgcttgg aggcatgttg ccttgcccta acgcttcaat
     8701 aaccatacca tcgatacctt cacgactata aaaactaaaa atgtcacctg gcatacccat
     8761 atatgctttt actaatggta catttagttt ctcattgaca ttttccaatg cttgttggcg
     8821 atatggcata tgatggaatt gcacacgatc ctttgtcaat acacctagcg gaccatgatt
     8881 tggactttga aatgtgtttg tattagacgt atgtgttttg gtaacattac gcgccgtatg
     8941 tatttcatca ttaaatacaa ccatcacgcc tttatgtcgg gccttttcat cagaggcaac
     9001 gcgaatagcg gaaatataat tatataatcc gtcagaacca atttcattag acgagcgcat
     9061 tgcgccagta ataacaacag gttgctcgat acctaatatt aaatcaagta aaaaggcagt
     9121 ttcttctaac gtatcggtac catgcgtgat aacgaaacca tcataatatt tatttgttac
     9181 tgcttctaaa ataatatcct ttaattgttt aacatgttgg attgtcatat gaggtgatgg
     9241 tacattaaaa ggatttaatt catctatttg tgcatattga tttatgacat cttgatgcag
     9301 tgaaataggg ttaatatcat ttgttactac tttattagat tggtcttgtg acatactaat
     9361 ggtgccacca gtatgaataa caagtagatg tttcatatat ttcctcctat atttcattta
     9421 cctaattatg ataaaatatt attcataaaa cgacaaggaa gggaaatgac gcatgaaagc
     9481 cattaatatt gcattagatg gtccagctgc tgccggaaaa agtacaattg caaaacgtgt
     9541 agccagtgaa ctatcaatga tttatgttga tacaggagca atgtatcgtg cattaacata
     9601 caaatattta aaattaaaca aaactgagga ctttgcaaaa ctagttgacc aaacaacatt
     9661 agatttaact tataaagcag ataaaggtca atgtgtcatt ttagataacg aagatgtaac
     9721 agacttttta agaaataatg atgtgacgca acatgtttca tacgttgcat ctaaagagcc
     9781 agtacgttca ttcgccgtta aaaaacaaaa agagttagct gcagaaaaag gtatcgtaat
     9841 ggatggtcgc gatatcggaa ctgtagtgct accagatgca gatttaaaag tatatatgat
     9901 tgcatcagtt gaagagcgag cagaaagaag atataaagat aatcaattaa gaggaatcga
     9961 atcaaatttt gaagatttaa aacgtgatat tgaagcacgt gatcaatatg acatgaaccg
    10021 tgaaatatca ccattaagaa aagcagatga tgcagtgaca ttagatacga caggcaagac
    10081 gattgaagaa gttactgacg aaattttagc gatggtgagt caaattaaat aatctagtaa
    10141 gtcataaata tctatttgta atgtgcaacg cttaatgcat atacaaattg aatgtgctga
    10201 taatgattta ctcaacttaa aaggtgattt ttattcaacg atgaatgaaa gttgcctttt
    10261 tacttttggt aaaagttaat gcgtcagtca agtgtgtatg tttttcaaaa agttaacaga
    10321 aataataaag gtaaattatt agaattccaa aaataattca ttaaattcat aaagcatttt
    10381 acaaatggta agaaaatgag tgttacaaat ctaagaattg caaaagaagc tgatttagtc
    10441 acaaaaaatg tcctatgtaa taattcgaga aagatgcact atatacggtc ttcttactat
    10501 tcaaatgtaa aagttgctta tttgcgtggc tttttgtttt ataaaagtat aaaattttac
    10561 tataatatat cttgt
//
LOCUS       DAKSQE010000087        10351 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.087, whole genome shotgun sequence.
ACCESSION   DAKSQE010000087 DAKSQE010000000
VERSION     DAKSQE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10351
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.087"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            243..1727
                     /locus_tag="O0V33_001978"
     CDS             243..1727
                     /locus_tag="O0V33_001978"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCX8976602.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQSVADQGCKLVVVNKEQSLSANVTQVVVPDTLRVASILAHTLFDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAENIQESL
                     QGVSFDFVTPFGTYQVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVGQ"
     gene            1708..1968
                     /locus_tag="O0V33_001979"
     CDS             1708..1968
                     /locus_tag="O0V33_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HCX8976603.1"
                     /translation="MALLVNKRLLDEGKTIDVYLFEALNDQIIIAIPDWFWSYQMAMT
                     LNEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            1968..3530
                     /locus_tag="O0V33_001980"
     CDS             1968..3530
                     /locus_tag="O0V33_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCX8976604.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSAFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEEVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFPEIKLYSLL"
     gene            3832..4635
                     /locus_tag="O0V33_001981"
     CDS             3832..4635
                     /locus_tag="O0V33_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCX8976605.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            4854..7178
                     /locus_tag="O0V33_001982"
                     /pseudo
     CDS             4854..7178
                     /locus_tag="O0V33_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
     gene            7195..8553
                     /locus_tag="O0V33_001983"
     CDS             7195..8553
                     /locus_tag="O0V33_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCX8976606.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVVAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMVLMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            8692..10203
                     /locus_tag="O0V33_001984"
     CDS             8692..10203
                     /locus_tag="O0V33_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCX8976607.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EEIGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIHLAFDNDITCVHVPMNGEKGLNGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNLEPGQRVMLDQIDSTKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYLISCSDNFVVKNR"
BASE COUNT         3728 a         1381 c         2003 g         3239 t
ORIGIN      
        1 tccagtatac aattatagca tatttttgga aacaattatg atattatacc atgttacaag
       61 atggttttaa taatttaaga tgaaccataa ttgtgaaact aattcataat accttatgtt
      121 ttatttttaa tagtagaaat tagaaaatgc tgattagtag gatataacag tgaaattata
      181 aatttattaa catcaacaaa acgtgtataa taaacatatt gtagaaaaag gagcggttca
      241 gtttggatgc aagtacgttg tttaagaaag taaaagtaaa gcgtgtattg ggttctttag
      301 aacaacaaat agatgatatc actactgatt cacgtacagc gagagaaggt agcatttttg
      361 tcgcttcagt tggatatact gtagacagtc ataagttctg tcaaagtgta gctgatcaag
      421 ggtgtaagtt ggtagtggtc aataaagaac aatcattatc agctaacgta acacaagtgg
      481 ttgtgccgga cacattaaga gtagctagta ttctagcaca cacattattt gattatccga
      541 gtcatcagtt agtgacattt ggtgtaacgg gtacaaatgg taaaacctct attgcgacga
      601 tgattcattt aattcaaaga aagttacaaa aaaatagtgc atatttagga actaatggtt
      661 tccaaattaa tgaaacaaag acaaaaggtg caaatacgac accagaaaca gtttctttga
      721 ctaagaaaat taaagaagca gttgatgcag gcgctgaatc catgacatta gaagtatcga
      781 gccatggctt agtattagga cgactgcgag gcgttgaatt tgacgttgca atattttcaa
      841 atttaacaca agaccattta gattttcatg gcacaatgga agcgtacgga cacgcgaagt
      901 ctttattgtt tagtcaatta ggtgaagatt tgtcgaaaga aaagtatgtc gtgttaaaca
      961 atgacgattc attttctgag tatttaagaa cagtgacgcc ttatgaagta tttagttatg
     1021 gaattgatga ggaagcccaa tttatggctg aaaatattca agaatcttta caaggtgtca
     1081 gctttgattt tgtaacgcct tttggaactt accaagtaaa atcgccttat gttggtaagt
     1141 ttaatatttc taatattatg gcagcaatga ttgcggtgtg gagtaaaggt acatctttag
     1201 aaacgattat taaagctgtt gaaaatttag aacctgttga aggacgatta gaagttttag
     1261 atccttcgtt acctattgat ttaattatcg attatgcaca tacagctgat ggtatgaaca
     1321 aattaatcga tgcagtacag ccttttgtaa agcaaaagtt gatattttta gttggtatgg
     1381 caggcgaacg tgatttaact aaaacgcctg aaatggggcg agttgcctgt cgtgcagatt
     1441 atgtcatttt cacaccggat aatccggcaa atgatgaccc gaaaatgtta acagcagaat
     1501 tagccaaagg tgcaacacat caaaactata tagaatttga tgatcgtgca gaagggataa
     1561 aacatgcaat tgacatagct gagcctgggg atactgtcgt tttagcatca aaaggaagag
     1621 aaccatatca aatcatgcca ggacatatta aggtgccaca tcgagatgat ttaattggcc
     1681 ttgaagcagc ttacaaaaag ttcggtggtg gccctgttgg tcaataaaag acttcttgat
     1741 gaaggtaaaa ctattgatgt ttatttattc gaagcattaa atgaccagat aatcattgct
     1801 ataccagatt ggttttggtc atatcagatg gcaatgacat taaatgaaga aacttgtttt
     1861 gaagcaatac tcatgcaatt gtttgttttt aaagaagagg aagaggcaga atcgattgca
     1921 tcacaactaa cagattggat agaaacatat aaaaaggaga aagactaatg aacttaaagc
     1981 aagaagttga gtctagaaag acttttgcga ttatttcaca tcccgatgca gggaaaacaa
     2041 cgttaactga aaaactattg tacttcagtg gtgccattcg tgaagcgggt acagttaaag
     2101 ggaagaagac tggtaaattt gcgacaagtg actggatgaa agttgaacaa gagcgtggta
     2161 tttctgtaac aagttcagta atgcaatttg attacgatga ttataaaatc aatatcttag
     2221 atacaccagg acatgaagac ttttcagaag atacgtatag aacattaatg gcagttgaca
     2281 gtgctgtcat ggtcatagac tgtgcaaaag gtattgaacc acaaaccttg aagttattta
     2341 aagtttgtaa aatgcgtggt attccaattt ttacattcat taataaatta gaccgagtag
     2401 gtaaagaacc atttgaatta ttagatgaaa tcgaagagac attaaatatt gaaacatacc
     2461 ctatgaattg gccaattggt atgggacaaa gtttctttgg catcattgat agaaagtcta
     2521 aaacaattga accatttaga gatgaagaaa atatattaca tttgaatgat gattttgagt
     2581 tggaagaaga tcatgcaatt acaaatgata gtgcttttga acaagcaatt gaagaattaa
     2641 tgttggttga agaagcgggt gaagcctttg ataatgacgc gctgttgagt ggagacttaa
     2701 cacctgtatt tttcggttca gctttagcta actttggtgt acaaaatttc ttgaatgcat
     2761 atgttgattt tgcgccaatg ccaaatgcga gacaaacaaa agaagaggtt gaagtaagcc
     2821 cgtttgatga ttcattttca ggatttatct ttaaaattca agccaacatg gaccctaaac
     2881 accgtgatag aattgccttt atgcgtgtcg ttagtggtgc atttgaacgt ggtatggatg
     2941 tcactttgca acgtactaat aaaaagcaaa agatcacacg ttcaacgtca tttatggcag
     3001 acgataaaga aactgtgaat catgctgtag caggcgatat cattggacta tatgatacag
     3061 gtaattatca aattggagat actttagttg gtggaaaaca aacctacagt ttccaagatt
     3121 taccacaatt tacgccagaa atttttatga aagtttctgc taaaaacgtc atgaaacaga
     3181 agcatttcca taaaggtatt gaacaattag ttcaagaagg tgcgattcaa tactataaaa
     3241 cattacacac aaaccaaatt attttaggtg ctgttggtca gttacaattt gaagttttcg
     3301 aacatagaat gaaaaacgaa tataatgttg atgttgttat ggagccagta ggccgtaaaa
     3361 ttgcacgttg gattgaaaat gaagaccaaa ttacagataa gatgaacaca tcaagatcga
     3421 ttttagtgaa agatagatat gacgatttag tattcttatt tgaaaatgaa tttgcaacaa
     3481 gatggttcga agagaaattc cctgaaatta aattgtatag tttactttaa cggctcaatt
     3541 gtataatcga atttgttaca ttaaaaataa ttgtttcctt gaagaaaaat aaattgtata
     3601 ttttaaaaga aaaaggtata ctatgatgta tcaaatgaat aacctatggc attttgtcag
     3661 aggggagtaa cttaagaatc atgaccgtat aaatgattcg acactttatc gtcattacga
     3721 agatatcttc cggtaaagtg ggcaattaaa attgcttagt gagacctttg ctatttattt
     3781 agcataggtc tttttgtttg tacttaactt atttatttaa aggagttgta catgttaatg
     3841 gatccaagtt tgatcttacc ttatttatgg gtacttgtcg ttttagtatt tttagaaggc
     3901 ttattagcag cagataacgc aatcgttatg gctgtaatgg ttaagcactt accacccgaa
     3961 caacgtaaaa aagctttgtt ttacggtttg ctaggtgcat ttgtatttag atttttagca
     4021 ttattcttaa ttagtattat cgcgaacttt tggtttattc aagctgcagg agcggtttac
     4081 ttaatttata tgtcaatcaa aaatctatgg cagttcttta aacacccaga aattgaaagt
     4141 cctgaagctg gagatgatca tcattatgat gagtctggtg aagagattaa agcaagtaac
     4201 aaatcattct ggggaactgt gttgaaaata gaatttgcag atatcgcatt tgccattgat
     4261 tctatgcttg ctgctttagc tattgctgta acacttccta aagttggtat tcactttggt
     4321 ggtatggact taggtcagtt cgtagtcatg ttcctaggtg gaatgattgg tgttattcta
     4381 atgcgttatg cagcaacatg gttcgtagag ctattaaaca aatatccagg acttgaaggt
     4441 gcagccttcg cgatcgttgg ttgggtaggt gttaagttag ttgtcatggt attagcgcac
     4501 ccagacatcg ctgtattgcc tgagcacttc ccacatggcg tattatggca atctattttc
     4561 tggacggtac taattggatt agtaattatc ggttggttag gttcagttgt taaaaataaa
     4621 aaatcgcata aataaatgat gtgaagcgga caatcttaat ttagtttaag gttgtccttt
     4681 ttcatttaat taagtgatct atgaaaaatg gattttgaag aatatgtatc aaaagatgcg
     4741 atatagtatt aagaaaatgt gccttttata tttagcattt tttcaataga aattatatag
     4801 attttaaagc aaattaagtg ttaatgtgtc ataatgaaaa gtgattttat tgaatggagt
     4861 ggacattagt ggatattggt aaaaaacatg taattcctaa aagttagtac cgacgtaagc
     4921 gtcgtgaatt cttccacaac gaagacagag aagaaaattt aaatcaacat caagataaac
     4981 aaaatataga taatacaaca tcaaaaaaag cagataagca aatacataaa gattcaattg
     5041 ataagcacga acgttttaaa aatagtttat catcgcattt agaacagaga aaccgtgatg
     5101 tgaatgagaa caaagctgaa gaaagtaaaa gtaatcaggg tagtaagtca gcatataaca
     5161 aagatcatta tttaacagac gatgtatcta aaaaacaaaa ttcattagat tcagtagacc
     5221 aagatacaga gaaatcaaaa tattatgagc aaaatactga agcgacttta tcaactaatt
     5281 caaccgataa agtagaatca actgacatga gaaagctaag ttcagataaa aacaaagttg
     5341 gtcatgaaga gcaacatgta ctttctaaac cttcagaaca tgataaagag actagaattg
     5401 attttgagtc ttcaagaact gattcagaca gctcgatgca gacagagaaa ataaaaaaag
     5461 acagttcaga tggaaataaa agtagtaatc tgaaatctga agtaatatca gacaaatcaa
     5521 attcagtacc aatattgtcg gaatctgatg atgaagtaaa taatcagaag ccattaactt
     5581 tgccggaaga acagaaattg aaaaggcagc aaagtcaaaa tgagcaaaca aaaacttata
     5641 catatggtga tagcgaacaa aatgacaagt ctaatcatga aaatgattta agtcatcata
     5701 caccatcgat aagtgatgat aaagattacg ttatgagaga agatcatatt gttgacgata
     5761 atcctgataa tgatatcaat acaccatcat tatcaaaaat agatgacgat cgaaaacttg
     5821 atgaaaaaat tcatgtcgaa gataaacata aacaaaatgc agactcatct gaaacggtgg
     5881 gatatcaaag tcagtcaagt gcatctcatc gtagcactga aaaaagaaat atggctatta
     5941 atgaccatga taaattaaac ggtcaaaaac caaatacaaa gacatcggca aataataatc
     6001 aaaaaaaggc tacatcaaaa ttgaacaaag ggcgcgctac aaataataat tatagtgcca
     6061 ttttgaaaaa gttttggatg atgtattggc ctaaattagt tattctaatg ggtattatta
     6121 ttctaattgt tattttgaat gccattttta ataatgtgaa caaaaatgat cgcatgaatg
     6181 ataataatga tgcagatgct caaaaatata cgacaacgat gaaaaatgcc aataacgcag
     6241 ttaaatcggt cgttacagtt gaaaatgaaa catcaaaaga ttcatcatta cctaaagata
     6301 aagcatctca agacgaagta ggatcaggtg ttgtatataa aaaatctgga gatacgttat
     6361 atattgttac gaatgcacac gttgtcggtg ataaagaaaa tcaaaaaata actttctcga
     6421 ataataaaag tgttgttggg aaagtgcttg gtaaagataa atggtcagat ttagctgttg
     6481 ttaaagcaac ttcttcagac agttcagtga aagagatagc tattggagat tcaaataatt
     6541 tagtgttagg agagccaata ttagtcgtag gtaatccact tggtgtagac tttaaaggca
     6601 ctgtgacaga aggtattatt tcaggtctga acagaaatgt tccaattgat ttcgataaag
     6661 ataataaata tgatatgttg atgaaagctt tccaaattga tgcatcagta aatccaggta
     6721 actcgggtgg tgctgtcgtc aatagagaag gaaaattaat tggtgtagtt gcagctaaaa
     6781 ttagtatgcc aaacgttgaa aatatgtcat ttgcaatacc tgttaatgaa gtacaaaaga
     6841 ttgtaaaaga attagaaaca aaaggtaaaa ttgactatcc cgatgtaggt gttaaaatga
     6901 agaatattgc cagtctaaat agttttgaaa gacaagcagt taaattgcta ggaaaagtta
     6961 agaacggtgt tgttgtagat caagttgaca acaatggttt agcagatcaa tctggtctga
     7021 aaaaaggtga tgtaattact gaattagatg gcaaactttt agaagatgat ttacgcttta
     7081 ggcagattat atttagtcat aaagatgact tgaaatcaat tacagcgaag atttatagag
     7141 atggtaaaga aaaagaaatt aatattaaac taaaataatg ttgaggtgaa caacgtgtca
     7201 atttttagcc agtttttaaa aagatcaagc cctcaacaag gtattgtatt gtattatatc
     7261 gtcgcaattg tcgttgcatt tttattatta aacttaccgt atgttcataa accaggtgta
     7321 gaagtaaatc caattgacac attatttgtt gccgtatccg gaattagtgt tactggattg
     7381 tctccgataa gtattgtcga tacctattct acatttggac aattaattat cctcgtgata
     7441 ttaaatattg gtggaattgg cgtcatggca attggtacga tgttatgggt ggtactaggt
     7501 aaacatattg gaattagaga acgtcagtta attatgttag ataataacaa aaacacaatg
     7561 agtggtaccg tcaaattgat tattgatatt gtaaaatcaa tatttgtaat cgaactcgta
     7621 ggagccatgt tattagcatt ttacttttat cgagataatc cagatttaaa atatgcaatc
     7681 atgcaaggtg tttttgtttc tatttctgcc actaccaatg gtggattaga tattacaggt
     7741 aagtcattaa ttccttatgc acatgattat tttgtacaag cgatagttat atttttaata
     7801 attttaggat caatcggctt cccagtatta ttagaggtta aagcttacat tcaaaatagg
     7861 gttactaatt ttagattttc attatttact aaaattacga catcaacata tttattccta
     7921 tttattgttg gggtattagc cattctatta tttgaacata accatgcgtt caaaggatta
     7981 agttggcatc aatcgttatt ctattcgctg tttcaatcag cgactacaag aagtgcgggt
     8041 cttcaaacaa ttgatgtgac aacactaagt gaccctacga atattatcat gggtatttta
     8101 atgtttatag gttcttcgcc aagttcggtt ggtggcggta ttcgtacaac aactttcgct
     8161 attttaattt tgtttttaat taactttagt aataatgccg ataaaacatc cataaaagta
     8221 tacaatagag aagtacacat tatggatatt caacgttcat ttgcagtatt tacaatggcg
     8281 acaattttaa catttttagg aatgctaatt atatcagcta ctgaaaatgg taagcttaca
     8341 tttttacaag tattttttga agtcatgtct gcatttggaa cttgtggact atcgcttggt
     8401 gtcacaagtg atattagtga tatttccaag gtcgtactaa tggtactcat gtttatagga
     8461 cgtgttggct taatatcatt tatcattatg atagcaggac gtcgagaacc agataaattc
     8521 cattatccaa aagaacgtat tcaaatagga taatataata gcaatctaag tttagttaat
     8581 gtagatttta actggaactt agattgcttt tttagtttgt atttttaact tatattataa
     8641 gacgattgga tttgaaaatg gtaaactagt aacaatgaga ggtgtaacat gatggaaaaa
     8701 aatgaaaaca ttaatgtaga gattttaact acgtcagata tgcatagtca tttcttaaat
     8761 ggtgattatg gttcaaatat ttatagagct ggtacttatg ttaaccaagt aagagcacaa
     8821 aatcatcgcg tcattttatt agatagtggc ggaagtttag ctggctcgtt agcggcctat
     8881 tattatgcta ttgttgcacc ttataaacga catccaatga taaagttgat gaacagaatg
     8941 cattatgatg ctagcggtgt gagtccaagt gaattcaagt ttggtttatc atttttaact
     9001 cgttcaattg ctttggcacg ttttccatgg ttatcagcaa atattgaata caatgttact
     9061 aaggagcctt atttttcaac tccatattgt attaaacatt ttggtgactt aaaaattgct
     9121 atcgtaggcg ttacagcaga tggtttaatg gaaaatgagt attctgaaat ggagcaagat
     9181 gtatctattg aaaagacatt agtggcatca aaacgatgga ttagatatat ccatgaagtt
     9241 gaagagccgg attttttgat tgtaatttat catggtggat tgaataaaat tagtaatagc
     9301 acgaaaaata aaaaggcaag ttcgaatgaa gctgaaaaat taatggaaga aattggtgtt
     9361 atagatttaa tgattacagc tcatcagcat caaacaatag taggtcaaga tcatgaaacg
     9421 tattatgttc aggctggtca agatgcaaaa gagcttgtac atctttcgat taattttaaa
     9481 aagagaacaa caacttatga tgttgaaagc attgattcta aagtgattga cttaaatgag
     9541 tatgaagagg accaagaatt attagattta acattctatg atagaaaagc agtggcttat
     9601 tggtcacagg aaatcataag tgataaaggt ttgatgttat cagtaaatgg gttacaagat
     9661 ttagtctgtc aaacacatcc attttcgcaa ttattacatg atgcaattca ccttgcattt
     9721 gataatgata taacatgtgt ccacgtgcct atgaacggag agaaggggtt gaatggacag
     9781 attcgaaatg aagatttgta tcatgcatac ccttatccag ataagccaat ggatatgaca
     9841 attagtggtc aaaatatcaa agatatattg gagtatagtt attcacattt agattttgtt
     9901 aacgagcaat taagcttaac aattattgat gaaacattat gtacaatgtg gcaaggattc
     9961 aattatgaga ttgatatgaa tctagaacct gggcaacgag taatgttaga tcaaattgat
    10021 tcgactaaga gttatagagt tacaatgact gactattgtt atcgtaacta caagaattat
    10081 ttaaaaaatg ctattataca tgaatcatac gatgaaacaa tgagtacatt aattgcagag
    10141 aagttaagag atccgaatta tcttatttca tgtagtgata attttgtagt taaaaacagg
    10201 tgaatattgc tatgtaataa attaattata agtttaacaa gtataaaaac ctttcttaga
    10261 gaagattatc taggaaaggt ttttagaatt ataaaattga tataaaagaa cagtgaaatt
    10321 ctcattcatt atatatataa gtaaacaaaa a
//
LOCUS       DAKSQE010000088        10197 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.088, whole genome shotgun sequence.
ACCESSION   DAKSQE010000088 DAKSQE010000000
VERSION     DAKSQE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10197
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.088"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            198..2081
                     /locus_tag="O0V33_001985"
     CDS             198..2081
                     /locus_tag="O0V33_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCX8976608.1"
                     /translation="MGMEAYKIEHLNKSYADKIIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMMDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAERELTLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LFEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREESEIAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQHSKSSTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            2093..3874
                     /gene="recQ"
                     /locus_tag="O0V33_001986"
     CDS             2093..3874
                     /gene="recQ"
                     /locus_tag="O0V33_001986"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCX8976609.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            4095..5072
                     /locus_tag="O0V33_001987"
     CDS             4095..5072
                     /locus_tag="O0V33_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8976610.1"
                     /translation="MIKFKNVTKRYDKHVAVDNISFDINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLEMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDDAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQQIEVLYQHLSESERVIVMQEDGVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            5065..6579
                     /locus_tag="O0V33_001988"
     CDS             5065..6579
                     /locus_tag="O0V33_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCX8976611.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYDQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKIKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            6819..7877
                     /gene="hisC"
                     /locus_tag="O0V33_001989"
     CDS             6819..7877
                     /gene="hisC"
                     /locus_tag="O0V33_001989"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCX8976612.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLDGILKEVNDDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPSHVPVLIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEHFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNGKMLEVLSNFKYE"
     gene            8036..8578
                     /locus_tag="O0V33_001990"
     CDS             8036..8578
                     /locus_tag="O0V33_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HCX8976613.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLREPGFFRHLKVMPHAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
     gene            complement(9130..10047)
                     /locus_tag="O0V33_001991"
     CDS             complement(9130..10047)
                     /locus_tag="O0V33_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCX8976614.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKAKDYAKDVDILFILGGDGTVNELINGVMTHDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILSANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPTMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
BASE COUNT         3754 a         1410 c         1864 g         3169 t
ORIGIN      
        1 attatatgtg ttgataaaaa ggtatattat aaaaatcatt agcatagtca tcaaagcctt
       61 atcaagtttt atttagagtt gaagcaacaa tatattaaat tatgacactt aacagtatcg
      121 atttcttttg atttcaaaaa ggtgtataat gtatagaact gatttaagta cgtagagatt
      181 tcaacagaag aaggaagatg ggtatggaag catataaaat tgaacattta aataaatctt
      241 atgccgataa gattatattc gataatctag atttatcaat ttcagaaggt gaaaaaatag
      301 gtttagtagg cataaatggt acagggaaaa gtacgttgtt aaaagtaatt ggtggtattg
      361 atgatgattt tacagccaat gttatgcatc caaatcaata tcgaattcgt tattcgtctc
      421 agaaacagga tcttaatgaa gatatgactg tttttgatgc agtattaagt tctgatacaa
      481 caactttacg catcatcaag caatatgaac aggcagtaca agcttatgcg gatgatcaaa
      541 gtgataaatt gttcaagcga atgatggatg cgcaagatgc tatggatcaa catgatgctt
      601 gggactataa cgctgaaatt aaaacgatcc tctcaaaact aggtatacat gatactacta
      661 aatacattaa agaattatcc ggcggacaac aaaaacgtgt tgtacttgct aaaacattaa
      721 tagaacaacc agatttattg ttattagatg aacctacgaa ccatttagac ttcgaatcaa
      781 tcagctggtt gatcaattat gtgaagcaat atcctcatac agttttattc gtaacccatg
      841 atcgatattt tttaaatgaa gtttccacta gaattattga actaaacaga ggtaagttag
      901 cgtcatatcc tggtaactat gaatcttata ttgaaatgcg cgctgaaaga gaactaacac
      961 ttcaaaagca acaacaaaag caacgagctt tatataagga agaacttgct tggatgaggg
     1021 ctggagctaa ggcacgtact acaaagcaac aagctagaat taatcgattt aatgacctag
     1081 aaaatgaagt taaccagcaa tataaagacg ataaaggtga attgaatctt gcttattcaa
     1141 gattaggtaa gcaagtgttc gaattagaag acttatcaaa ggctattaat gataaagtat
     1201 tatttgaaca tctgacggaa attattcaaa aaggtgagcg tattggtgtt gttgggccaa
     1261 atggagctgg taaaacaaca ctcttaaata ttttgagtgg agaagaccaa caattcgaag
     1321 gtaaattgaa gactgggcag acggttaaag tagcttattt taagcaaaca gatgagaccc
     1381 tggatagaga tattcgtatg attgattatt taagagaaga aagtgagatt gcaaaagaaa
     1441 aagatggaac ctcggtatct attacacaac ttcttgaacg atttttattt ccaagtgcaa
     1501 ctcatggtaa aaaagtttat aaattatctg gtggagagca aaagcgtttg tatttattac
     1561 gtctactcgt acaccagcca aatgttctgt tgttagatga accgacaaat gatttagata
     1621 ctgagacttt aacaatactt gaagattata ttcatacttt cggtggtaca gtgattaccg
     1681 taagccatga tcgctacttc ttaaataaag ttgcacagtc atattggttt attcatgatg
     1741 gtcagatgga aaagattatc ggaacttttg aagattatga aagttataaa aaatcattag
     1801 ataaaaataa atccacattg aagcaacact ctaaatcttc tacaactgta cgtaagaaaa
     1861 atggtttatc atataaagaa aaattagaat atgaacaatt gatgaaacgc atagaacaag
     1921 cggaagtaag aatggaagaa attgatgtgc tcatgattga ggcaagtgca gattatggga
     1981 aaattaaaga attaaacgaa gaaaaagaac aacttgaaat tcaatatgat ttagacatca
     2041 caagatggag tgagttagaa gaaattaaag aacaacaata aggggtcaat ttatgatgca
     2101 acaaacatta tcgcattact ttgggtatga aacgtttcga ccaggacaag aagaaattat
     2161 tagcaaagta ttagaccatc gtaatgtgct tggtgtctta ccaactggtg gaggtaagtc
     2221 tatatgctat caagtaccag gtttattgtt aggtggtaca acaattgtaa taagtccact
     2281 aatatcatta atgaaagatc aagtggatca attaaaagcg atgggaattc aagctgcttt
     2341 tttaaatagt agtttgactc aaaaagagca acaacgtatt gaaaaagcat tatcaaatgg
     2401 agaaattcaa tttttgtatg ttgcaccaga acgatttgaa aaccgatatt ttttaaatat
     2461 gcttcagcgt ataaagattc atttagtcgc gtttgatgaa gcgcattgta tatctaaatg
     2521 gggacatgat tttagaccga gttatcaaaa cgttatttct aaagtattta cgttacctca
     2581 agattttaca ataatagcgt tgacagcaac tgccacggtt gaagtacagc aagatattag
     2641 agaaaagtta aatatcgctc aaactgatca aattaaaacg agtactaagc gtagaaactt
     2701 aatttttaaa gtaaatccta cttatcaacg tcaaaaattt atattggatt atattaaaac
     2761 acacgatgaa gatgcaggca ttatttattg ttctacacgt aagcaagttg aagagcttca
     2821 agaagcctta gaaagccaga aaattgaaag tgttatatat catgcaggtt tgagcaataa
     2881 agaaagagaa gaagcgcaga atgatttctt atttgatcgt gttaaagtag tcgttgctac
     2941 aaatgctttt ggcatgggta ttgataaatc caatgtacgt tttgttattc attataatat
     3001 gcctggagat ttagaatctt attatcaaga agcgggtcgt gcaggtcgtg atgggttgaa
     3061 aagtgaatgt attttgttat ttagtgaacg cgatatcaat ttacacgagt attttataac
     3121 agtctctcaa gctgatgatg actataaaga taaaatgggc gaaaagttaa ctaaaatgat
     3181 tcaatataca aaaacaaaaa aatgtttaga agcaacaatt gtccattatt ttgaaccgaa
     3241 tgaaaaatta gaagaatgtg aacaatgtag taattgtgtt caacaagata aatcatataa
     3301 tatgacacaa gaagctaaga tgattattag ttgcatcgct cgtatgaaac aacaagagag
     3361 ttatagtgtt atcattcaag tgctaagagg agagtcaaca gattatatta agtataaagg
     3421 ttatgatcaa atttcaaccc atggtttaat gaaaggctac acaacatcag aattaagtca
     3481 cttaatagat gaattaagat tcaaagggtt cttaaatgaa aatgatgaaa tattaatgtg
     3541 tgatacttca attaaaaaat tactcagtaa tgaagtagaa gtattcacaa caccatttaa
     3601 gcaaaaagcg actgaaaaag tatttataaa tacggttgaa ggggttgacc gagtattatt
     3661 cagtcaattg gtagaagttc gtaaaaagtt aagtgacaaa ttaacgatag cacctgtaag
     3721 tatattttct gattacacgt tggaggaatt tgctaaacgt aagcctgctt cgaaacaaga
     3781 tatgattaat attgatggcg taggtagtta taaattaaaa cattattgtc cagcattttt
     3841 agaaacgatt caaaattata aagccaaagt ataatgaagg cacctagtaa acattccttg
     3901 ttttactggg tgttttttga atttaaagta aaataacact atttcttaac ttgagattac
     3961 ctattattca ttattttgtt aaaaaagttc aaggataaat catttaaatt tgaaaattgt
     4021 aataaacgtg gttaaatagg gaatacatac atataatctc ggttttatgc tatgagaaaa
     4081 aaagaggtgg caaagtgatt aagtttaaaa atgtaactaa gcgttatgac aaacatgttg
     4141 ctgtcgataa cattagtttc gatattaatg agggtgaatt ctttgtacta attggacctt
     4201 caggttgtgg aaaaactacg acattaaaaa tgattaatcg actcattcac ttaagtgaag
     4261 gttatattta ttttaaagat aaaccaataa gtgattatcc agtatacgaa atgcgttggg
     4321 atattggata cgtattgcag cagattgcat tattcccaca tatgacaatc aaagaaaata
     4381 ttgcacaagt gccacaaatg aaaaagtgga aagaaaaaga tatagataaa agagtagatg
     4441 aattacttga aatggttgga ttagaacctg aaaaatataa aaacagaaaa cctgatgaat
     4501 tgtcaggggg acaacgacaa cgtgtaggag ttatacgtgc gttagcagct gatccaccag
     4561 ttattttaat ggatgaaccg tttagtgcat tagacccaat cagccgagaa aaacttcaag
     4621 atgatttaat tgaattacaa actaaaatta agaagacaat catatttgtt acacatgata
     4681 ttcaagaggc gatgaaactt ggtgataaga tttgtctttt gaatgaaggt catattgaac
     4741 aaattgacac accagaagga tttaaaaata atcctcaaag tgaatttgtt aaacaattta
     4801 tgggtagtca tttagaagat gatgcgccat gtgttgaaga gaatgcgatt atccgtgact
     4861 tggatattat gaaaccaatc gatgaggtta catctatgag cgcttatcca attgtttatg
     4921 acaatcaaca aattgaagta ttgtatcaac atttatcaga gagcgagcgt gtcattgtca
     4981 tgcaagaaga tggcgtaggt caatatgtta ttgataggaa agatatcttc aaatatttgt
     5041 cccagaaaaa ggaggtagct caacatgact aactttttcg acatattgag tgagcgtaag
     5101 gggcaactat tttcgacaat gatagaacat attcaaatat catttatcgc attattgatt
     5161 gcaactgcta ttgcggtacc attaggtatt ttattgacga agactaaaac gatatctgaa
     5221 atcgtaatga atattgcggc aattcttcaa accataccat cgttggcatt attaggttta
     5281 atgattcctt tatttggtat cggtcgtgtg ccagcaatta tcgcacttgt agtgtatgca
     5341 ttgttaccaa ttttaaggaa tacgtatact ggaattaaag aagttgatcc atcgctcatt
     5401 gaagcggcta aaggtatagg tatgaagcca tttagacgtt taactaaagt tgaacttcca
     5461 atagcaatgc ctgttataat ggctggtgta agaacggcta tggtattaat tataggtaca
     5521 gcaacactag cagcattaat tggtgcaggc ggactaggag atttaatttt attaggtata
     5581 gaccgtaaca atgcatcatt gatattatta ggtgcaattc cagcagcctt attggcaatt
     5641 atatttgatt taattttaag atttatggct aaattatctt ataaaaagtt attgatgacg
     5701 ttaggtgtta tagtgatgat tattatactg gccatcgcta ttcctatgtt tgcacaaaaa
     5761 ggtgataaaa ttacgttagc tggaaagctt ggctccgagc cttcgattat tacaaatatg
     5821 tataaaattt taatagaaga agagaccaaa aatactgtag aagtgaaaga tggtatgggc
     5881 aaaacagcat ttttatttaa tgctttaaaa tctgacgata tagatgggta tttagagttt
     5941 actggaacag ttttaggtga attaacaaaa gaaccattga agtcaaaaga agagaaaaaa
     6001 gtatatgatc aagctaagca aagtcttgaa aagaaatatc aaatgactat gttaaaacca
     6061 atgaaatata acaatacgta tgctttagct gtaaaacgtg attttgctaa acaacataat
     6121 atacgtacaa ttggtgattt aaataagatt aaagatcaac ttaaaccagg atttacattg
     6181 gaatttaatg atcgtccaga tgggtacaaa gctgttcaaa aggcttataa tttaaattta
     6241 gataacatac gtacaatgga acctaagttg agatatcaag cgattaataa aggtaatatt
     6301 aatttaattg atgcatattc aactgacgct gaattaaaac aatatgatat ggttgtgtta
     6361 aaagatgata agcacgtatt tccaccatat caaggagcac cattatttaa agaaagcttt
     6421 ttaaagaaac atccagaaat taagaaaccg ttaaacaaac tagaaaacaa aatatctgat
     6481 gaagatatgc aaatgatgaa ctataaagta acagttaaaa atgaagaccc atatacagtt
     6541 gcgaaagatt atttaaaagc aaaagggtta atcaaataac gaccaacgcc acataagatg
     6601 aggggcacgc cattatatct tatgtggcgt tgctatattt acatctatgt ttatgttcaa
     6661 tttaaacatg caatactgat taaaaaatat gacatgttaa acacaatgta agctagtaat
     6721 gtgtgaaaat agtagcattg cattttagaa acatagagcg atataatgaa tataagtttt
     6781 ttaaaatttc agttaattct aaggaggttg tttttattat gaaagaacaa cttaatcaac
     6841 tatcagcata tcagcctggt ttatcaccaa gggcattgaa agaaaagtat ggcattgaag
     6901 gagatttata taaacttgca tcaaatgaaa atttgtatgg accatcgcct aaagttaaag
     6961 aagcgatatc agcacactta gatgagttat attattatcc tgaaacagga tcaccgacat
     7021 taaaagcggc gattagtaaa catttaaatg tagatcaatc acgcatttta tttggtgcgg
     7081 gattagatga agttatatta atgatttcta gagcagtatt aacgccaggg gacactattg
     7141 ttacaagtga agcgacattc ggtcaatatt atcacaatgc gattgttgaa tcagctaatg
     7201 tgatacaggt acctttaaaa gatggtggct tcgacttaga tggcatttta aaagaagtta
     7261 atgatgacac gtcattggta tggttatgta atccaaataa tcctacaggt acgtatttta
     7321 atcatgagag cttagattcg tttttatctc aagtaccatc acatgttccg gtactcatag
     7381 atgaagctta ttttgaattt gtgacagcag aggactaccc ggatacactt gctttgcaac
     7441 aaaaatatga caatgcattc ttattacgta cattttcaaa ggcgtatgga ttagcgggtt
     7501 tacgtgtagg atatgtggta gcaagcgaac atgcgattga aaaatggaac atcattagac
     7561 caccatttaa tgtgacacgt atatctgaat acgcagcagt tgcagcactt gaagatcaac
     7621 aatatttaaa agaggtaaca cataaaaata gtgttgaacg tgaaagattt tatcaattac
     7681 ctcaaagtga gcatttctta ccaagtcaaa cgaattttat atttgtaaaa acaaagcggg
     7741 taaatgaact ttatgaagca cttttaaatg tagggtgtat tacgcgacca tttccaactg
     7801 gtgttagaat tacaattggt tttaaagaac aaaatggtaa aatgttagaa gttttatcaa
     7861 actttaaata cgaatagtaa gtgggcacca catggtatga atatgtgaca aaatgcagtg
     7921 tgaatgatga ggtgagagta ttcagtgtaa aaagcaacaa tagatgatat tgttttgtat
     7981 caattgcttt tttgctatac tgaatcaata ctgatatttt gaggagaaga ttaaaatgac
     8041 ccgtaaatca atcgcgattg atatggatga agtattggca gatacattag gagaaatcat
     8101 tgatgctgtc aattttagag cggatttagg tattaaaatg gaagctttga atggtcaaaa
     8161 acttaaacat gttattcctg aacatgatgg attaattaca gaagtattaa gagaaccagg
     8221 cttcttcaga caccttaaag tgatgccgca tgcacaagaa gttgtaaaaa aattaactga
     8281 acattatgat gtatatattg ctacagcagc aatggatgta ccaacatcat tcagtgataa
     8341 atatgaatgg ttactagagt tctttccatt cttagatcca cagcattttg ttttttgtgg
     8401 tagaaaaaac atcgttaaag ctgattattt aatagatgac aatcctagac agcttgaaat
     8461 ttttactggt acaccgatta tgtttacagc agtgcataat attaatgatg atcgatttga
     8521 acgcgtaaat agctggaaag atgtagaaca gtatttttta gataatattg agaaataaaa
     8581 atatgtcact cgaagccttt catgtagcaa ataaaatgca tatactgggg ccccaacaaa
     8641 gagaatttcg aaaagaaatt ctacaaacaa tgcaagttgg ggcggggccc caacataaag
     8701 aaatactttt tctttagaaa ttagtatttc ttatgcatga gtgtaactca tgcaatccta
     8761 tttttaagta cacattagct gtgactaatg ataaagaatc gctacataat caaccattag
     8821 tcgttcttta tcatttccat cccactctca ataaatgtta gtctatctca ttattataaa
     8881 tcggatgaat gtgttaatct atggcagatt acacttcatc cgatttttta tagaatttga
     8941 aaaaggcaca taaaccacta tgatttaaaa tacaacatca atcattttag tggcatgcgc
     9001 caaaattata tgtctgtttt tgaaacaggg taatagctta aagctaatac caacaaatat
     9061 tacgtacgtt gaatcttatg attacacttt aaacctaata taatatcgcg ttaatatcat
     9121 tccggatgct tacaaatcat tgacagtaag caactgaatg gcatttggta taacctcaat
     9181 atcaataggt gtttctaatg aaatttcgcc atcaatatca actttcattg ttggatctgt
     9241 tgtaagtgaa atctttttac caggtatatg ctcaatacct tgagtaattt cattccaatt
     9301 catactatca cgctttttaa aaatatcatt taaaatactg aaactttgtt cattaaaaat
     9361 gaaagtgttc agttcaccat cttgaggaga caaatcagtc aatggtattc gactaccacc
     9421 aatgaatgga ccatttgctg atagtatcat ggtcgtttcg ccagaatatg tcttatcatc
     9481 aattgataat tgataattaa attgtgttgg atttagcagt gttttgacag ttgatccaat
     9541 ataactcaat ttaccaaata tatcttttga accatcttgt acgttttcag cgttttgaac
     9601 aattagacct aagccaacaa agttgagtgc atattgatta tttattttaa ttacatcgta
     9661 tgtaccaact tgtgcagaaa tcatttgttc actagcttgt ttatgattag gtgctatatt
     9721 tagcgttttt gtaaaatcat taaaagtacc gcctggtaaa atgccaatag ggagttgaag
     9781 gtcgtgtgtc ataacaccat ttataagttc gttaaccgtg ccatcaccgc caagaataaa
     9841 taatatatct acatcttttg cataatcttt agctttaatt tcttggcaat atttaattat
     9901 gtcaccttcg ttttcactca attgaataga aagatgctta caaattgaac ttaatgccgt
     9961 tgtaacttcc ccgatacctt gattaatatt ttttaatcca ctgtgttcat ggtaaaagag
    10021 gacaccatgt gtatatttat tttccatagt ttagcctact ttctaaaaat tggttcatta
    10081 aatatatata cccactttta attgttaata ccaaaaatat gtttttaaat agagaaaatg
    10141 gtaataactg aaattgatgt ctatagagtg ggacgagaaa atatagttat agttgtc
//
LOCUS       DAKSQE010000089        10122 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.089, whole genome shotgun sequence.
ACCESSION   DAKSQE010000089 DAKSQE010000000
VERSION     DAKSQE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10122
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.089"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            325..3777
                     /locus_tag="O0V33_001992"
     CDS             325..3777
                     /locus_tag="O0V33_001992"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCX8976615.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSSTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFDIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKAAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATD"
     gene            complement(4045..4956)
                     /locus_tag="O0V33_001993"
     CDS             complement(4045..4956)
                     /locus_tag="O0V33_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HCX8976616.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
     gene            5407..6318
                     /locus_tag="O0V33_001994"
     CDS             5407..6318
                     /locus_tag="O0V33_001994"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCX8976617.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLLGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
     gene            6343..6804
                     /locus_tag="O0V33_001995"
     CDS             6343..6804
                     /locus_tag="O0V33_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HCX8976618.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREIDKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            7131..8168
                     /locus_tag="O0V33_001996"
     CDS             7131..8168
                     /locus_tag="O0V33_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCX8976619.1"
                     /translation="MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRS
                     EQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSY
                     YIVSTKREEIVSVYATGENVNVSPLKIGQHSAEIFNHTSINPEPSFTVDGKKYEFELS
                     DEDLKTQTLIKYGNIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYRLNSNSTSEEHNK
                     RPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTE
                     DALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVM
                     RDYYSLIFLEK"
     gene            8184..8618
                     /locus_tag="O0V33_001997"
     CDS             8184..8618
                     /locus_tag="O0V33_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCX8976620.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(8685..9614)
                     /locus_tag="O0V33_001998"
     CDS             complement(8685..9614)
                     /locus_tag="O0V33_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCX8976621.1"
                     /translation="MKKVNRNILNAVLVGAGFVSSLLMINKNKVITKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAGYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQRSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGINLTSSRFIKWLNDMNVIPGYYGVNSI
                     DLMNDLYQKGAHTIVTDRPDLAQQFKQSINNKQ"
     gene            9853..10107
                     /locus_tag="O0V33_001999"
     CDS             9853..10107
                     /locus_tag="O0V33_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCX8976622.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
BASE COUNT         3526 a         1490 c         1835 g         3271 t
ORIGIN      
        1 atcactaaat gtggtggcga aattagaatc atgatgataa gataaagtta tcgctattga
       61 taataaatgt tgatgtttta caattttatt tttaattttt tagagttgta gaaatcgaaa
      121 atgtatcaca acaaaaacgt gaactacctg ttgttgcata atcctttttg aaattattat
      181 ttttgaagcg gaggttttaa ttgtctcaag aatgttaaat tttgaaaatt tgaaacataa
      241 ctatagcaaa acagagcgct ttaagataaa attttattat ctaattagtt agaaaattct
      301 aatagtttga ggagtgtatc gctattgaaa caaataaaaa agttacttgt tgctaaccgt
      361 ggagaaattg caattcgtat attcagagcg gcagcagaat tagacatcag cacagttgca
      421 atttattcga atgaagacaa aagttcatta catagatata aagcagatga atcctattta
      481 gttggaagtg atttaggtcc tgctgaaagt tatttaaata ttgagcgtat cattgatgta
      541 gcaaaacaag cgaatgtgga tgcgattcat cctggctatg gatttttaag tgaaaatgaa
      601 caatttgcgc gtcgttgtgc agaagaaggg attaaattta ttggtcctca tttagaacat
      661 ttagatatgt ttggagataa agttaaagct cgtacaacgg ctatcaaggc agatttacca
      721 gttattcctg gtacagacgg tccaattaaa tcatatgaat tagcaaaaga atttgcagaa
      781 gaagctggtt tcccgctaat gattaaagcc acaagtggtg gcggcggtaa aggtatgaga
      841 atcgttcgtg aagaaagtga attagaagat gctttccata gagcaaaatc agaagcagaa
      901 aaatcatttg gtaatagtga agtttacata gaaagataca ttgataatcc aaagcatatt
      961 gaagtacaag tcataggtga cgaacatgga aatatcgtac acttatttga acgtgattgt
     1021 tcagtacaac gtcgtcatca aaaagttgta gaagttgcac catcagttgg attatcatca
     1081 acattacgtc aacgtatttg tgatgctgca attcaattga tggaaaatat taaatatgtc
     1141 aatgcaggta ctgttgaatt tctagtatct ggtgacgaat tcttctttat agaagttaac
     1201 cctcgtgtac aagtagagca tacaattaca gagatggtaa caggaattga tattgttaag
     1261 acacaaattt tagttgcagc aggtgccgat ttatttggtg aagagattaa tatgccgcaa
     1321 caaaaagata ttacaacatt aggctatgcc atccaatgtc gtattacaac agaagatccg
     1381 ttaaatgatt ttatgccgga tactggaaca atcattgctt atcgttcaag cggtggcttt
     1441 ggtgtacgtc ttgatgctgg agatggtttc caaggtgctg agatatcacc ttattatgat
     1501 tcattactcg taaaattatc tacacatgcg atatcattta aacaagcaga agaaaaaatg
     1561 gtacgttcat tacgagaaat gcgtattcgt ggtgttaaaa ctaatattcc attcttaatt
     1621 aatgtaatga agaataaaaa gttcacaagt ggtgattaca caactaaatt tattgaagaa
     1681 acaccagaac ttttcgacat tcagccgtct ctagatagag gtactaaaac attagaatat
     1741 ataggtaatg taacaattaa tggtttccca aatgttgaga aacgtccgaa accagactat
     1801 gaattagcat caattccaac tgtatcttca agtaaaatcg cttcatttag tggtacgaaa
     1861 caattgcttg atgaagtagg tccaaaaggt gtagctgaat gggttaaaaa gcaggatgat
     1921 gtcttactaa cagatacaac ctttagagat gcacaccaat cattattagc tacacgagtt
     1981 agaactaagg atatgattaa tatcgcatcc aaaacagcgg acgtatttaa agatggtttc
     2041 tcactagaaa tgtggggcgg tgctacattt gatgtggcat ataatttctt gaaggaaaac
     2101 ccatgggaac gacttgaacg tctacgtaaa gctattccaa atgtattatt ccaaatgttg
     2161 ttacgtgctt caaacgcagt tggttataaa aactatcctg ataatgttat tcataaattc
     2221 gtacaagaaa gtgctaaagc aggcatagat gtctttagaa ttttcgattc attaaactgg
     2281 gtagatcaaa tgaaagttgc caatgaagca gtacaagaag cgggcaaaat ctcagaaggt
     2341 actatttgtt atacaggtga cattttaaat cctgagcgat caaacattta tactttagag
     2401 tattatgtca aactagctaa agagttagaa cgtgaaggtt tccatatttt agcgattaaa
     2461 gatatggcag gcttattaaa acctaaagct gcttacgaat tgattggtga gttaaaagca
     2521 gctgtagatt taccaatcca tcttcacact catgatacaa gtggtaatgg tttattaacg
     2581 tacaaacaag caatagatgc tggtgtcgat atcattgata ctgctgttgc ttcaatgagt
     2641 ggtttaacaa gtcagccaag cgccaattcg ttatattatg cattaaatgg cttcccacgt
     2701 caccttagaa ctgatattga aggtatggag tcacttagtc attattggtc aactgtacgt
     2761 acttattatt cagactttga aagtgatatc aaatcaccga atactgaaat ttatcaacat
     2821 gaaatgcctg gtggacagta ttcgaattta agtcaacaag ctaaaagttt aggtttaggc
     2881 gaaagatttg atgaagtcaa agatatgtat cgcagagtga atttcttatt tggtgatatc
     2941 gtaaaagtaa cgccatcgtc taaagtagtt ggtgatatgg cactttatat ggtacaaaat
     3001 gatcttgatg aacaatccgt tattacagat ggctataaat tagatttccc agaatcagta
     3061 gtgtcgttct tcaaaggtga aataggacaa cctgtaaatg gttttaataa agatttacaa
     3121 gcggttattt taaaaggcca agaagcatta acagctcgtc caggtgaata tctagagcca
     3181 gttgattttg aaaaagtccg tgagttgctt gaagaagagc aacaaggtcc tgttacggag
     3241 caagatatta ttagttatgt attatatcca aaagtatatg aacaatatat tcaaactaga
     3301 aatcaatacg gaaacttatc gttacttgat acgcctacat tcttctttgg aatgcgtaat
     3361 ggtgaaacag tagaaatcga aatcgataaa ggtaaacgat taattattaa actagaaacg
     3421 attagtgaac cagatgaaaa tggtaataga acgatttact atgcgatgaa tggtcaagcg
     3481 agacgtattt atattaaaga tgaaaatgtg catacaaatg cgaacgttaa gccaaaagca
     3541 gataagagta atccaagtca tatcggtgcg caaatgccag gttcagtaac tgaagtcaag
     3601 gttagtgtag gtgaaactgt gaaagctaat cagccgttgc taattactga agctatgaaa
     3661 atggaaacaa caattcaagc accatttgac ggtgtgatta aacaagtaac tgtaaataat
     3721 ggtgacacaa tagcgacagg cgatttatta atcgaaattg aaaaagcaac tgactaaaat
     3781 gattaaaata aaacgagatt acacaacaat tgacgtgtaa tctcgttttt acttgtctta
     3841 ggtttaattt taaatattga tataaaaagg gagtgggaca gaaatgatat tttcgcaaaa
     3901 tttatttcgt cgtcccaccc cggcaaggtt gactagaatt gaaaaaagct tgttacaagc
     3961 gcattttcgt tcagtcaact actgccaata taactttgta gagcattgaa cattgattta
     4021 tgtcacagct tctttgcttt tttgttattg cttgtcactt cttactgatc gtaacattag
     4081 catgataaag tatgttgtca taccaaataa ataagtgata aataatgcat ggaatagtgc
     4141 tattatcaaa ttaacattag tcataataga caatgcacct gtgataactt gtaatataac
     4201 aagtataaat gcagctgtat aaccataatg aacagtacgg ttatttggat aatttttaac
     4261 agcgtgaata taagtaatca taataatcgt aaacactata aaagccatga tacgatgcgt
     4321 gagttggacc caatcttgtt ctgaatgtgg tacaagatcg tggaatggca atggccaacc
     4381 accatatgct aaacttgcat ccgcatgtcg cactagtgca ccagtgtaaa caccacaata
     4441 aatgatgatt gccattaacc atgttaaacg tcttaatggc tttttgatat ataattcgtc
     4501 agcttcatat ttttgatcta tagagaaaat aatcaatgtt attaaaaata cagatgagaa
     4561 actgattaat gatataccaa agtgcaatgc taaaacgtaa tcgttttgtt gccaaataac
     4621 agcagcagct ccgattaatg cttgcaataa taagaatcca acactaatga ttgataaagg
     4681 tttaatttct ttaatatagc ctatatgttt ccatgcagtg ataactaacc ataagaccat
     4741 taataaagac aaagctgaaa cggctctatg acttaattca ataatcgtat caataggaaa
     4801 gaattctgga atcaacgcac catgacatag tggccaagaa gaaccacaac catcagctga
     4861 tccggttttg gtaactaagg ctccaccaag ttgtacaaat gtcatcatta acgttgctac
     4921 gacacctaac cattttaaat tctttttgcc aaacaattta tacaccccat cataaaaaaa
     4981 cgcatcactg agattttgat ttaatagctg aacttgagtt aatactatga atgaacctaa
     5041 cagttggatt tgaagagatg ggatccaaca cagtttatat aatttaattg tttctagtat
     5101 tagaagtgat cgactatcat caatgatatg aataacacga caatgatgtg ccgttcactg
     5161 ctataaaatt tcagtcacag ttactcaaat ttgaagctta aatattaatc tcttttagat
     5221 gcatgattct acttattaaa attttctaac atatacatta tagcaataaa ttaaatgtta
     5281 tttgtgtcac aaaattgaca ttttctcttg tcgttaattt gtcacaattt aattttatta
     5341 aacctagtct ttttatgcaa tctaagatat cataatctta tatgaacaaa tttaaggagg
     5401 gggattatga gcaaagagca tactttgtca caaaatatta gcagagttaa cttcaaagaa
     5461 ttgcaacaga taattaaaat gggacttgtt caaggtaact taattccggc ttttgcgggc
     5521 gcatggctag cagttgtaat gacaaatcat tccttcttat catcaatacc tcaaatttta
     5581 ttaatgctat taggatctac tttaattatg ggtggcgcat gtgcgttaaa taattattac
     5641 gaccaagata ttgatcgtat tatgcctagt aaacaaaata gaccaactgt aaataataga
     5701 attacagatc aaaatttatt acttttaagt tttggtatga tgttagttgg agaaatttgt
     5761 ttgtttttat tgaatatacc atcaggcgta cttggtctta tggggattgt aggttatgtg
     5821 tcttattact caatatggtc taaaagacat acaacatgga acacagtgat tgggagtttt
     5881 cctggagcag taccaccact aattggatgg gttgcaattg aaggacaaat tagtttaaca
     5941 gcgattgcgc tgtttttagt tgtattttgt tggcaaccaa ttcattttta tgccttagct
     6001 attaaacgta aagatgaata tgcacttgca aatattccaa tgttaccatc agttaagggc
     6061 tttaaacgta cacgtgtcag tatgtttatc tggttgatta ttttattgcc agtgccttta
     6121 ttactaataa atttaggtgt cgtattcgta gtgttagcta cattattaaa tttaggatgg
     6181 attgcattag gtttaacaac atttaagaaa aattcagatc aaacaaaatg ggcaacacaa
     6241 atgtttatat attcactaaa ttatttggtg atctttttcg tgttagctgt gattgtttca
     6301 ttacttactt tgatctagat taattaagtt aggatgaaaa atatgggcgt tccaatttta
     6361 ccaacgataa gtacgacatg tattgtcatt agtgcaattt taattgccat tggttggaga
     6421 cttatttgga aaagggaaat agataaacac aaaaatgtta tgttagctgc agctgttttt
     6481 gctttaactt ttttcttaat ctatgcaagt agaacgattt ttatcggtaa tacagcattt
     6541 ggcggaccag catcaattaa gaaatattat acaattttct tatttttcca cattaattta
     6601 gcaacaattg gtggtattct aggtctagtt caaattatta ctgcatttaa agataaatat
     6661 aatgtgcacc gcaaattcgg gccattcgct tcagttatat ggttctgtac tgcaattaca
     6721 ggtgtagcag tttacttatt attatatgta ttatatccag gtggagaaac gacatcactg
     6781 attaaagcaa catttggtca ttaatacgct tttacaaatg atactaatct gataaggcaa
     6841 tttgttttat caggtttttt gatgaagaat agtcggacag ttgatggtat tcatttgata
     6901 ccttaactgt ccgttttttt atacatattt taatcctaat aatgaaattc aacctgaaaa
     6961 cataaagata ggttatgata tagactgtag ggaaaatctt tatgatgtta acattgtgcc
     7021 tgaagatggt tttgtttcat cgaaatgaca cagttatgtc agtgttgaaa atttgctaat
     7081 taatgtgttg ttttttacaa tagtattaat actttttagg tggttgattg atgaaaaagt
     7141 taattataag agtcgtcgga gtattatttt tagttggatt tttaatatat cttttttatt
     7201 caccgagatt aaaatttgat gtattagaga acccgaataa aggtaataaa gtaaatagat
     7261 ctgaacaggt gaataaatca aataaccatg ctgaaaatcc aaagcctaaa gaaggtgttg
     7321 gtacatgggt aggtaaagac attaaagtgc ttacttctaa atttggacaa gcagatcgtg
     7381 tctacccttt tagagatggt tacaaaaatt atgtgtttaa agacaaaaac agttattaca
     7441 ttgtttcaac taaacgtgaa gaaatcgttt cagtgtatgc tacaggtgag aatgtcaatg
     7501 ttagtccgtt aaaaatagga caacattctg cagaaatttt taatcataca agtattaatc
     7561 cagaaccgtc ctttacagtt gatggtaaaa aatatgaatt tgaactttca gatgaagatt
     7621 taaaaacaca aacattgatt aaatatggca acatatatgc acaagtgtat tctgatcaac
     7681 aatcgaaaaa ggtgctaagt gtacgatttt taacaaaaga aatgttagca gatattgaac
     7741 cttatcgatt aaattctaat tctacgtcag aagagcataa taagcgtcca gttgagcaaa
     7801 atccaaatca attaatttct ctttatgaag taacgaatga aatgagaaaa ttaaaaggat
     7861 taaaaccatt gaaaatcaat agcgatttag cacatattgc atctaataac ttatatgaag
     7921 cgacctctaa tggttctgat agtgttgaat ttacagagga cgcattaaga gggcaattag
     7981 ataaaaatca tgttacttat aaaacaactg ctcaaaatgt tggttatgcg tttaatgatg
     8041 taccaacatt aatccatagt tggatgaatt cagatataca tcgatctcgt ctattaaatt
     8101 caaaatacga tgagatgggt ggagatgtaa tgagagatta ttattcacta attttcttag
     8161 aaaaataaca atgaggggta tttatgatta atgaagcttc actagcgata ttagatgata
     8221 ttgatgaact ggctgatatg atagttgcat cagatattta tgcatcattt gagcaagcta
     8281 aacaggcgct cgaaaacaat gacgaagccc atttacttta tcaatcattt ttaaaatcaa
     8341 aagagaaata tgatgaagta atgcgtttcg ggaaatatca tcctgattat aagaaagtca
     8401 tgttagagac aagacaacgc aaaagagcat atgagatgct tgacgttgtg atgcattaca
     8461 aagctaggga aatggcactt caacatttaa ttgatgaagt tgttacgaaa attgcgtacg
     8521 ctgtttcaga acatgtcaaa atagaaacag gcaatccatt ctttcaaaca tcacatagtg
     8581 gttgtgcgac gggaggatcc tgtaattgtt cattataaaa aacatcgagt cagaaaaaga
     8641 tggttattga aaccatttac tagcatctga ctcgatgttt ttgtttattg tttgttgttt
     8701 attgattgtt tgaattgttg tgctaaatct ggtcgatctg tcacaatcgt gtgtgcacct
     8761 ttttggtata aatcattcat cagatctata ctatttacgc cataatagcc tggaatgaca
     8821 ttcatatcat ttaaccattt gataaaacga gatgaagtca aattaatacc tttaaaatga
     8881 gtaggcattt ggaacgtttg tgctaatggt tggtagtacc taccacctaa taaatgatat
     8941 tttaaaaatg cctctgtaac ttcctgttgg ctagcaccaa ttgcaacaga tctttgtgca
     9001 attttattaa aacgtacgat ttgttcttta taaaaacttg tcacaagaac acggtcaaat
     9061 gcttgattct ctgcaattgt atcaaacata atttgtggtg cgattgagcc ttcataggat
     9121 tcaggagcat cctttaagtc tacgtttata tacatatcag gatattgctt cagcaactca
     9181 tcgaaggtta gtatagctgt gtgtgcatga ccacgatatg gtgttagtcc attgatatct
     9241 ttgaagtgat aacctgcgtc taattttttt aattctgcta atgtatgggc actgactttt
     9301 ccagagccgt tcgtcgttct atcgactgtt gcgtcatgaa aaacgataag ctgttgatct
     9361 tttgtgagtc tcacatctgt ttcaaaacca tcaacgccta attgttttgc atagtcaaat
     9421 gcaagttgcg tttgctctgg tcttaaagcc ataccaccgc gatgcgcaaa tatatatggt
     9481 gcattgcctt tgaaaaaagc agggatggtt tgctttttag taatcacttt atttttattt
     9541 atcattaaca aactacttac aaatccagca ccgactagta ccgcatttaa aatgtttctg
     9601 tttacttttt tcataaaaaa ttcctcctcg tttactttta tagcttaaca aattcatagt
     9661 tctgactaca gcaaaatgtg cggttaatca tatagtgttg ctcttgagaa ttgaattaat
     9721 ataatgatgc ttaatataat ctaaatacat ttatcgtgca attacataag acataattaa
     9781 ggtagcttaa tattttaaaa ttaataaaga catgatatga ttaatgctga aaataaatgg
     9841 agtgaggttt aaatgaattt aatcccaaga actagtattg tagtttattt aaaacatatg
     9901 aaacatgaac gacaaatccg aaaatatgga catatcgttc attcaaatag agatcgtaaa
     9961 tttgtaatta tgtatgtgaa tgagcaagat gttgatcaaa ttgtacataa actaatgcaa
    10021 cttaaatacg ttagacacat tgatggctca ccatataaat acttaaagaa aacttacgaa
    10081 aaagagaaac acgaaatata taattaatat tattgaagtt ct
//
LOCUS       DAKSQE010000090        10110 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.090, whole genome shotgun sequence.
ACCESSION   DAKSQE010000090 DAKSQE010000000
VERSION     DAKSQE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10110
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.090"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            190..1710
                     /locus_tag="O0V33_002000"
     CDS             190..1710
                     /locus_tag="O0V33_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCX8976623.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELIEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
     gene            complement(2066..2761)
                     /locus_tag="O0V33_002001"
     CDS             complement(2066..2761)
                     /locus_tag="O0V33_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCX8976624.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTAENATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
     gene            complement(2761..5418)
                     /locus_tag="O0V33_002002"
     CDS             complement(2761..5418)
                     /locus_tag="O0V33_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HCX8976625.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKLGYIEPHQRDETNALAEQLPKIATNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGVHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KSRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KRRDFFNKPLIDHLMSFEHSYKIEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSYIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYAITKKQLYRTDLLFQFN
                     DSIKQTYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNKTQQHEQA
                     LSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESM
                     LNELSRAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSH
                     SKDMSIIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEID
                     MILERPHLKKRITVSSSVDLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYA
                     SDEQIEFAVIDEGPGISIEEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKH
                     QSNLQYKPNQPHGSIFYFNIDTDFNEGDI"
     gene            5556..5666
                     /gene="kdpF"
                     /locus_tag="O0V33_002003"
     CDS             5556..5666
                     /gene="kdpF"
                     /locus_tag="O0V33_002003"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCX8976626.1"
                     /translation="MLLCCKEVMAMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            5690..7366
                     /gene="kdpA"
                     /locus_tag="O0V33_002004"
     CDS             5690..7366
                     /gene="kdpA"
                     /locus_tag="O0V33_002004"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HCX8976627.1"
                     /translation="MEIILFLTMMVMIAYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGVITFVLLIVQQWLFLNPNHNLNQSIDLAFNT
                     AISFLTNSNLQHYNGESGVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIHGANMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPLGGLGPMVLMMLNVVFGGEGVGLMNLLIYVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMISSSLVN
                     KKSYHQDKHTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            7385..9412
                     /gene="kdpB"
                     /locus_tag="O0V33_002005"
     CDS             7385..9412
                     /gene="kdpB"
                     /locus_tag="O0V33_002005"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCX8976628.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNPKQLIKNPIMFVVEVGMLL
                     TLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQS
                     NLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESA
                     PVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIAL
                     FTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGM
                     DRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMMEKLIVAA
                     YMSSIYDDTPEGKSIVRLAKQMYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGA
                     PNSMINLVKQQQGNIPLNIESICMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVE
                     RFAELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIV
                     AMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMT
                     RGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIV
                     ALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFLGIKLIDIIVQFFV"
     gene            9432..9992
                     /locus_tag="O0V33_002006"
     CDS             9432..9992
                     /locus_tag="O0V33_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCX8976629.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIA
                     RVKHHVKFDNSNVTIDAATSSGSGLDPHITVENALKQAPRIADARHISTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
BASE COUNT         3325 a         1487 c         1864 g         3434 t
ORIGIN      
        1 tacatcttat ctaatgctag ctttttgaca aaaatatgac aatcaattaa tgtgattcta
       61 ataaatattc gcaaattgct ttattgcgat taaatttttt tggtggtact atatagaagt
      121 tgatgaaata ttaatgaact tatatgcaaa agtatattga gaaataaaca ggtaaaaagg
      181 agaattattt tgcaaaattt taaagaacta gggatttcgg ataatacggt tcagtcactt
      241 gaatcaatgg gatttaaaga gccgacacct atccaaaaag acagtatccc ttatgcgtta
      301 caaggaattg atatccttgg gcaagctcaa accggtacag gtaaaacagg agcattcggt
      361 attcctttaa ttgagaaagt agtagggaaa caaggggttc aatcgttgat tttagcacct
      421 acaagagaat tggcaatgca ggtagctgaa caattaagag aatttagccg tggacaaggt
      481 gtccaagttg ttactgtatt cggtggtatg cctatcgaac gccaaattaa agccttgaaa
      541 aaaggcccac aaatcgtagt cggaacacct gggcgtgtta tcgaccattt aaatcgtcgc
      601 acattaaaaa cggacggaat tcatactttg attttagatg aagctgatga aatgatgaat
      661 atgggattca tcgatgatat gagatttatt atggataaaa ttccagcagt acaacgtcaa
      721 acaatgttgt tctcagctac aatgcctaaa gcaatccaag ctttagtaca acaatttatg
      781 aaatcaccaa aaatcattaa gacaatgaat aatgaaatgt ctgatccaca aatcgaagaa
      841 ttctatacaa ttgttaaaga attagagaaa tttgatacat ttacaaattt cctagatgtt
      901 catcaacctg aattagcaat cgtattcgga cgtacaaaac gtcgtgttga tgaattaaca
      961 agtgctttga tttctaaagg atataaagct gaaggcttac atggtgatat tacacaagcg
     1021 aaacgtttag aagtattaaa gaaatttaaa aatgaccaaa ttaatatttt agtcgctact
     1081 gatgtagcag caagaggact agatatttct ggtgtgagtc atgtttataa ctttgatata
     1141 cctcaagata ctgaaagcta tacacaccgt attggtcgta cgggtcgtgc tggtaaagaa
     1201 ggtatcgcgg taacatttgt aaatccaatt gaaatggatt acattagaca aattgaagat
     1261 gctaatggta gaaaaatgag cgcacttcgt ccaccacatc gtaaagaagt acttcaagca
     1321 cgtgaagatg acatcaaaga aaaagttgaa aactggatgt ctaaagagtc agaatcacgc
     1381 ttgaaacgca tttctacaga gttgttaaat gaatataacg atgttgattt agttgctgca
     1441 cttttacaag agttaataga agcgaacgat gaagttgaag ttcaattaac ttttgaaaaa
     1501 ccattatctc gcaaaggccg taacggtaaa ccaagtggtt ctcgtaacag aaatagtaag
     1561 cgtggtaatc ctaaatttga cagtaagagt aaacgttcaa aaggatactc aagtaagaag
     1621 aaaagtacaa aaaaattcga ccgtaaagag aagagtagcg gtggaagcag acctatgaaa
     1681 ggtcgcacat ttgctgacca tcaaaaataa tttatagatt aagagcttaa agatgtaatg
     1741 tcttgagctc ttttttgttt tcaataactg attctatgta gatatcaaaa tactaacatt
     1801 ttaaaggtta aatatttaat tggattgaga gctgtgtgcg gttatatcat tctgtttaaa
     1861 tataaatatg aatctagaat ataacaacca ctaaaactaa gaataattga ccaaaaataa
     1921 attttatgaa agtaatttcg gtatcatgtg cttgtaaaaa acaatagggc attttgctga
     1981 cactagtgcg caataaataa taagttgaat ataaaaaaga tcactgccaa tcattcgttt
     2041 aatggcagcg atctttttta tttaattatt tctctttcca ctgcaacatt tgataaccaa
     2101 tgcgtggatg tgttttaata atatcttttg cgtcctcgtg acattgtgaa agtttttgtc
     2161 ttaatgatgt catatgcact cttaaactag gcatttcagt cttattaaca tagccatata
     2221 ttcgttttaa tatcatctca taagtgagta cttttccttt atgatttgac aatagttcta
     2281 acaagctaaa ttcatttggt gtcaaatgta cctcctgatt attaataaca acagatttgg
     2341 agccaaagtc gatgcttaac aaaccgttag taaatacaat gttagtttct tgatgtgact
     2401 tagcgattct ttcgatgact cgaattcgtg cccgaagctc atcaacatta aaaggcttag
     2461 tcatatagtc attcgcaccg ttatctaaag cttgaataat tgtttgttct tcttgtcttg
     2521 cacttattac aatgatagga atgtcagtat gttgcctgat ttctgaaatc aaacataatc
     2581 catctttatc tggtaaacct aaatctaata aaatgacatc tggtttatca atttgaattt
     2641 taaagtgtgc ttgtgtggca ttttcggctg tagttacatt gtaataatct aaagttaatg
     2701 caacatcaag taaatgtgtg attgcgtgat catcttcaat tatcaatatt ttagattgca
     2761 ttatatgtct ccttcattaa agtctgtatc tatattgaaa tagaatatac tgccatgtgg
     2821 ttggttcggt ttatattgta ggtttgattg atgtttgtgt aggatagtct gtactaaata
     2881 taagcctagt cccatgcttt ctttttggtt atctttaaaa tatttatttg accctgtgta
     2941 aaaaggctcg aatatctttt gttgttcttc tatactaatt ccaggtcctt cgtctataac
     3001 agcaaattcg atttgttcat cgctagcata acgaatagat aaattgattt tggtgtcagt
     3061 agaagtgtgt ttaactgcat tttcaatcaa attgaataaa gcttgtaaaa tcaacttact
     3121 gtcaatgtgt ataaactgta aatctactga ggatgataca gttatacgct tttttaaatg
     3181 gggacgttct aaaatcatat cgatttcttc tactaattca ctaacgagat aaggttgcaa
     3241 ttttatctga acatttgatg actgtaattt tgttaatgat aaaatatttg tcactaatag
     3301 atataaatac tgactttctt gaaaactatg cacaagtaat tgttcctttt ctatgataga
     3361 catatcttta ctatgtgaca ctaaaatatc taaatttccc ataattgttg ttaacggtgt
     3421 acgtatgtca tgcgaaattg atcttaaaaa atttgaatgt gtcagttgac gttcagcctg
     3481 taacatggat tctcttgttt gtttaagcaa cgtcacattt tcaacggcgc gagaaagttc
     3541 atttaacatt gattctaata ttgatgcatc atatggatta atcacttgag aactttggta
     3601 atcaatggct agaatgcctt taatcggaga tgtgccaatt ggtatcaacc atttattaat
     3661 gcctggaaat gtatctgttg ttgcaccagc ttgtctttca tttttaatta cccagcttaa
     3721 tgcttgttca tgctgttgag tcttattatc gatatggttt tgcaatggta ttgttttaat
     3781 tactttggat tgattgataa cgtatatagt aattgattgt tgcaataatt gattaatttg
     3841 gtatccagca tttattagta agttttcaac tgtataagtt tgtttaatcg aatcattaaa
     3901 ttgaaataat aaatctgtac gataaagttg ctttttagta atggcgtatt ggaatttaat
     3961 ttgttttaat aaagcactcg ttaaaatact tgttaaaatg ctgacgataa atgtaatagg
     4021 atagtcaaag cggtatactt caaatgtata tctaggttcc gtaaaaaaat aattaaaaac
     4081 aaatacgtta ataattgctg ctaaaaagcc aatgatgtag gaccgcgtcc aaatggataa
     4141 caaaatgatg ccgatgagaa aaatcattaa aataatcgta ctagactcat gcttatcaag
     4201 ttgataaatc cacagtccca tcattacaca aattatttga atcagaatca tttttaacat
     4261 atctatggcg aaccgtttgc ctttaggacg atagggtgat ttgttcattt tcaattcaac
     4321 aggtatttgt ttgattggaa cgatttcgat tttatagcta tgttcaaagg acattaaatg
     4381 gtcaattaaa ggtttattga aaaagtctcg ccgcttattt ctaatatgtt gtccgataat
     4441 taatttggtt acatcttgat ttttacacca ttcgtctaat cctaatgcaa cggtttggct
     4501 ataaactact tttacttttg ctcctaaaga ttttgcaagc atgagatgtt gatgcacttg
     4561 cttttgacta tctttatatt gcctgctttt ttcgaataca tctatataaa tagcagtgaa
     4621 ttttgcatgt tctttttgag caatatggaa tgcctcttta attactgctt cattataaat
     4681 actcccacta attgccacag caatatgagg tttgagtgac gttttatgat tgtgtcggac
     4741 tttttcttta tcactcatca agtcggcaac tgttcttaac gtcaatgtac gcagttcgct
     4801 taggtgggca tacgtaaaga aattactaaa tgctacatct agcctatcct ttttatatac
     4861 cttgccagct tttaagcgtt taattaattg ttcaggtgag atatctacga cttctaatac
     4921 atcggcgctc attatgaaat agtcgggtac acgttcttta acatgtacac cggtcatcag
     4981 ttcaatttga ctacttaaac tttcaatatg ttgaatgttc aaagtagtat gaacatcgat
     5041 accatgattt aaaatttctt caatatccat atatcgtttc tcatgacgat ctctagaaat
     5101 gttcgtatgt gctaactcat cgataagtac tattgtcggc gattcttcga ttatgcggtc
     5161 gacatctaaa tattgaaaat gatgactacc atgtttagta aaattggttg cgattttagg
     5221 taattgttca gctaatgcat ttgtttcatc acgttgatgc ggttcaatat atcctagttt
     5281 aatatcaacg ttactttgaa atagttcaat ggcatttgaa agcatctcaa atgttttacc
     5341 aacacctgga ctgtaaccga tgtaaattgt taaagatcca cgcttttttc ctatgtttag
     5401 cgattcagtg tttgacataa ccttcacctc gatagcaata tcataatatt cttcacaatt
     5461 aaatgctata aaatttattt ctgcatacac atcttaatga tttcttaata actaagtttg
     5521 aaaattaatg tttctctcat aaacaagttg ttacgatgtt gttgtgttgt aaggaggtta
     5581 tggcaatgct catcgtttta ttattggtag ttattgcatt agttttgtat ttattttatg
     5641 ccttaattcg tagtgaaaaa ttttagaaat atattaagtg agggttatca tggaaataat
     5701 tttgtttcta acaatgatgg ttatgattgc atatgttttt agtggatatc tatatcgcgt
     5761 agcattggtg caatcgtcaa gagtagattt aatatttact agatttgaaa atatgtgttt
     5821 taaaattatc ggcacagatt tagaacacat gtcagctaaa acatatgtca aacattttct
     5881 ggcatttaat ggatttatgg gggttataac atttgtattg ctaatagttc agcaatggct
     5941 ttttttaaat ccaaatcata atttaaacca atcgatagat ttagcgttta atacagcaat
     6001 atctttttta acaaatagta atttacaaca ctataacggt gaatcaggtg tgacatattt
     6061 aacgcaaatg attgtaatga catatttaat gtttacatct agtgcatcag gttacgccgt
     6121 ttgtatagcg atgttgagac gtttaaccgg attgactaat atcattggta atttttatca
     6181 agatattgtt cggtttattg tccgagtgct tttaccatta tcatgtttaa ttagtatttt
     6241 attgatgact caaggtgtac cacaaacgtt gcatgctaat ttaatgattc ggactttaag
     6301 cggacatatt caacatattg catttggacc tattgcatca cttgaatcaa taaaacatct
     6361 tggtacgaat ggtggaggat ttttagcagg aaattctgca acaccttttg aaaatccaaa
     6421 tatttggagc aattttatag aaatgggcag tatgatgtta cttcctatgt caatgttgtt
     6481 cttatttggt cgcatgttaa gtagacatgg taaacgagta catcgtcatg cgctgatatt
     6541 atttgtcgca atgtttttca tttttatagc aattcttaca ttaactatgt ggagtgagta
     6601 tcgtggtaat ccaatactag caaatttagg aattcatgga gcgaatatgg aaggtaaaga
     6661 ggtacggttt ggagcaggtt tgtcagcact atttacagtt attacgacgg catttacaac
     6721 gggttctgtt aataacatgc atgatagctt aacgcctcta ggtggattag gaccaatggt
     6781 attaatgatg ctaaatgttg tatttggtgg cgaaggcgta ggactcatga atttattgat
     6841 atatgtctta ctgacagtgt ttatatgcag tttgatggtt ggtaaaacgc cagaatattt
     6901 aaatatgcca attggcgccc gtgaaatgaa atgtattgtc ttagtctttc tcatacatcc
     6961 aattttaatt ttagtatttt cagcacttgc ttttatgatt cctggagcaa gtgaaagtat
     7021 aacgaatccg tcttttcatg gtatctcaca agttatgtat gaaatgacat cagctgctgc
     7081 gaacaatgga tcagggtttg aaggactgaa agatgataca acattctgga atatctctac
     7141 aggaatcatt atgttgcttt ctcgttatat accaattatt ttgcaattaa tgatttcatc
     7201 aagtttagtg aataaaaaat cataccacca agataaacat acaatagcga ttgataaacc
     7261 ttattttggt gtatcactaa tcgtatttat cgttttacta agtggtttga catttattcc
     7321 agtactatta cttggtccta ttggtgaatt tttaacttta aaataaagta aagggatgga
     7381 aaaaatgcat catgtaaata aatatttcaa tcaaacaatg gtgatagaag ctttgaaaat
     7441 gagtttttat aagctgaatc ctaaacaatt aataaaaaat ccgataatgt ttgtcgtcga
     7501 ggttggaatg cttttaacgt tgatattaat atgttttcct gacatatttg gaactagcta
     7561 tctatcgcga ggttatttga ttacaatttt tatcatatta ttaataacta tattgtttgc
     7621 taatttttca gaagcctttg ctgaaggtcg tggaaaagcg caagctgata gtttaagaca
     7681 agcacaatca aatttaactg ctcgattgat agaagaaaat ggtgcgtatc ggatagtgaa
     7741 cgccacagag ttaaaagccg gacaaaatat tcgggtagaa aatggagaaa caatccctgc
     7801 tgatggcgtt gtaataaatg gattagcaac cgttgatgaa tctgcaatta ctggtgagtc
     7861 tgcacctgtg ataaaagaat caggtggcga ttttgatgga gtcattggcg gtacacttgt
     7921 aacatctgat tggttagaaa tacgtgttga gagtgaagcg ggtacatctt ttttagataa
     7981 aatgattgcc ttagttgaag gggcggaaag aaataaaaca ccaaatgaaa ttgcactttt
     8041 tacattatta acaacgttaa caattatatt tttagtcgtt attgtaacat tatatccaat
     8101 agcatcttac ctgcatttaa ttttacctat tgcgatgctc attgcgttaa cagtatgttt
     8161 aataccaacg acaatcggtg gtttgttatc ggcgattggt attgcaggca tggatcgtgt
     8221 tactcaattc aatgtattag cgaaaagtgg gcgtgctgta gaagtatgtg gagatgtcga
     8281 tgtaatgatt ttagacaaga caggcacaat cacttatggc aatcgaatcg ctagtgaatt
     8341 tttaccagtg aatcaacaaa tgatggaaaa attgatagtc gctgcttata tgtcatcaat
     8401 ttacgatgat acgccagaag gaaaaagtat tgtgcgatta gcgaagcaga tgtatataaa
     8461 tgagctgcct aaagacattg atggcacata taagcctttt acagctgaaa ctagaatgag
     8521 cggaatcatt acaaatgaaa tatcggtatt caaaggtgca cctaatagta tgataaatct
     8581 tgtcaaacaa caacaaggca atattcctct taatattgag tctatctgta tggatgtatc
     8641 gagtaaaggt ggcacaccat taatagtcat tgaaaacaac gtcatgcttg gcgtgattta
     8701 tttaaaagat gtgattaaag atggtcttgt agaacgattt gctgaattga gaaaaatggg
     8761 cattgaaaca gttatgtgta cgggcgataa tgctctaaca gcagctacaa ttgcaaaaga
     8821 agcgggtgtt gatagatttg ttgcagagtg taaacccgaa gataaaatta aagtcatcaa
     8881 agatgaacaa gcgaaaggcc atattgttgc gatgacagga gatggtacaa atgatgcacc
     8941 tgcgctagca caagcgaata taggattagc tatgaactca gggacaatta gtgctaaaga
     9001 ggcggccaat ctaattgatt tagattcgaa tcctacaaag ttaatagagg ttgtaaaaat
     9061 aggcaaacaa ctattaatga cgagaggtgc attaacgaca tttagtttgg ctaatgacgt
     9121 agcaaaatac tttgcaattt tacctgcact aatgatgtca acgattcctg aaatgacttc
     9181 actaaatatt atgcatttat cgtcacctaa atctgcaatt atatcagctt tgatattcaa
     9241 tgcattaatc attgttgcct taattccaat tgccatgaaa ggtgtaaaag taaaggggta
     9301 cagcattgat agaattttta taaataatat gttgatttat ggattaggtg gattaatcgt
     9361 cccgttttta ggcattaaat taatagatat aattgtacaa ttttttgtgt gaagaggaag
     9421 gagtctgtac catgaatacg ataagaaata gtatttgttt aacgataata actatggtat
     9481 tgtgtggatt tttattcccg ttagctatca cgttaattgg tcaaatattt ttttatcaac
     9541 aagcaaatgg tagtttaatt acgtatgata atcgtatagt tggatcaaaa ttgattggac
     9601 agcactggac agagacacga tattttcacg ggagaccgag tgcagttgat tataatatga
     9661 atcctgaaaa attatataaa aacggcgtat catcaggtgg aagtaatgaa tcgaatggaa
     9721 acaccgaatt aatagcacgc gtgaaacatc atgttaaatt tgataattca aatgtaacaa
     9781 tagatgctgc aacaagttct gggtcgggct tagatccgca tattacggtt gaaaatgcat
     9841 tgaagcaagc acctcgtatt gctgatgctc gtcatatatc tacttcaaga gtggccgact
     9901 tgattcaaca tcgtaagcag cgtggtgtgt taacaaatga ttatgtgaat gtgcttgaac
     9961 taaatattgc tcttgataag atgaaagatt gaatatagat gaaagacatc atgttgataa
    10021 aattataaca tggtgtcttt tttaggtatt caagtacttt ctgcttaact aaatttttag
    10081 atgtaccttt aggaagaata aattagcatg
//
LOCUS       DAKSQE010000091        10109 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.091, whole genome shotgun sequence.
ACCESSION   DAKSQE010000091 DAKSQE010000000
VERSION     DAKSQE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10109
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.091"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(1..640)
                     /locus_tag="O0V33_002007"
                     /pseudo
     CDS             complement(1..640)
                     /locus_tag="O0V33_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1837 domain-containing protein"
     gene            complement(742..1221)
                     /gene="rlmH"
                     /locus_tag="O0V33_002008"
     CDS             complement(742..1221)
                     /gene="rlmH"
                     /locus_tag="O0V33_002008"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCX8976630.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEILGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVSQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(1589..3907)
                     /gene="adsA"
                     /locus_tag="O0V33_002009"
     CDS             complement(1589..3907)
                     /gene="adsA"
                     /locus_tag="O0V33_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HCX8976631.1"
                     /translation="MKALLLKTSVWLVLLFSAMGLWQVSSAAEQHTPMKAHAVTTIDK
                     ATTDKQQVPPTKEAAHQSGEEAATNVSASAQGTADEINNKVTYNAPSNKPSTAVSTTV
                     NETRDVDTQQASTQKLTHTTTFKLSNAETASLSPRMFAANAPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKITFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDMNKPSG
                     KRINAIQILNKETDKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANLAKYDTTEPQRMLLGKPAVSEQPAKGQQGSKGSESGKDTQPIGKDKVMNPA
                     KQPAPSKVVLLPTNRGTVSSGREGSDRALEGTAVSSKSGKQLASMSAPKGSTHEKQLP
                     KTGNNQSSSPAAMFVLVAGIGLIATVRRRKAS"
     gene            complement(4134..4934)
                     /locus_tag="O0V33_002010"
     CDS             complement(4134..4934)
                     /locus_tag="O0V33_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCX8976632.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAGHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            complement(5322..6110)
                     /locus_tag="O0V33_002011"
     CDS             complement(5322..6110)
                     /locus_tag="O0V33_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCX8976633.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPASILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGSEYQLSEISSGSVKYEQTYDNFPILNNSKAMLNFNI
                     EDNKATSYKQSMMDDIKPTDGSDKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            complement(6111..7445)
                     /locus_tag="O0V33_002012"
     CDS             complement(6111..7445)
                     /locus_tag="O0V33_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976634.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLSSDFTLFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGSNQIQVTWCEKGVFDYRRSLLRTDVVLNSEDNKTLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            complement(7438..9264)
                     /gene="walK"
                     /locus_tag="O0V33_002013"
     CDS             complement(7438..9264)
                     /gene="walK"
                     /locus_tag="O0V33_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCX8976635.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQSNKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            complement(9277..9978)
                     /locus_tag="O0V33_002014"
     CDS             complement(9277..9978)
                     /locus_tag="O0V33_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCX8976636.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
BASE COUNT         2806 a         2015 c         1520 g         3768 t
ORIGIN      
        1 ttttcatcaa ttttatgaaa aaccgtccca gctagaataa tatttttatc agaagtggta
       61 ccattttcat agctatcatg aagaatttct aagaatgcgt ctttttcgtt gctgtcattc
      121 atacaaattt caacatcatt aattgcgtta ttccaaagtt gagtattatc tgattcacat
      181 agtcttctac tcaaatcatt tttagcaact cttaggcgtt ctttaatttt cttgtttatg
      241 tcacttcttt tacttactgt acccgctttt gattctatta tccacatatt acaagtttca
      301 ctatctctaa gtataatatc aaaacctttt ttgctgaagc tctttcttct ttattaaaaa
      361 aaggtgaaac tattgaaaaa ttttttagaa atattcttac taaaacattc actaacaatt
      421 ctcctataaa tcctttctgt ttatcaatat catttggata tctttctaaa agttctttta
      481 tagtagaatt tacactatac attttgtagg ttttatttat tttatttcca taacatatat
      541 atacaatttg cttgtttatt tctcttttca aatcgtcact taaattttca ataataaaaa
      601 tatttatatt gtcaaatatt tctcttgtaa ctccatccat ttaattcaca ccttcattca
      661 ttgaaagttt caaatttatt tccctaagtt attcatcgtt ctgctttatt tatattaatc
      721 aaaataatta ataaatcccc cctatttatg atacgcctct cctcgcataa tcttaaacgc
      781 tctatacact tgctcaatta acacaacccg catcatttga tgtgggaatg tcattttgct
      841 gaatgatagt gcgtagttac tgcgttgtga gacgtccttg tgcagaccgt ttgatccgcc
      901 aatgacgaat acaaagtcgc tttgcccttg ggtcatgcgt tggttcaatt cttgggccaa
      961 tccttcggaa gatagcatct ttcctagtat ttctaatgta atgactgtgg attgtggttt
     1021 gattttggct agtatacgtt ggccttcttt ttcttttact tgctcaattt ctttgtcgct
     1081 catattttct ggtgcttttt cgtctggaac ttctatgatg tctatcttgg tatatgggcc
     1141 taaacgtttt tcatattctg ctatggcttg cttccaatat ttctctttta gtttccctac
     1201 agctaaaatg gtgattttca tgtcgtttgg tcctccaaat tgttatcaac tttccagtta
     1261 tccacaagtt attaacttgt tcacactgtc ccctcttatt ataccaatat tttttacagt
     1321 ttttgatatt ttcctgacat ttatgcccga tttttagtgt ataacttgtg tatttgtgga
     1381 taactctgtg ggcattgtgg gtatttctct ttaaattgta cacaattctg gataactatc
     1441 cccatttctg tggataaata ccttgatgtc ttattactta tccccgtttt tatatcccat
     1501 atttatcaac ggttatcgct tttttccata aaaaaacact accgtacctc ttatttaaga
     1561 aatacagtag tgttattttc catatatttt agctagcttt tctacgtcgt acagtcgcaa
     1621 ttaaacctat acctgctact aatacaaaca tcgctgctgg gcttgaactt tgattatttc
     1681 cagtttttgg taactgtttc tcatgtgtgc tacctttagg cgctgacatg ctagccaatt
     1741 gtttcccact cttacttgat acagcagttc cttccaatgc gcgatcagaa ccttctctac
     1801 cactactaac agttcctcta ttcgttggca acaacacaac tttacttggc gctggttgtt
     1861 tcgctggatt catcactttg tctttaccaa ttggttgtgt atctttacca gactcactac
     1921 ctttgctacc ttgttgtcct ttagctggtt gttcacttac tgctggttta cctaataaca
     1981 tacgttgtgg ttctgtcgta tcatacttag ctaagttagc tgtttttaaa taacttgcta
     2041 gtacttgatc taatgaaata ccttcttctc taggaccacc gaacatacta tatccgtcgc
     2101 cacctgatgc tgtgaagtca ttcatcgtta cgtgatatac acgttttaaa tcaatatttt
     2161 caaacttatc cgtctcttta tttaaaattt gaatagcatt aattcgtttg ccagatggtt
     2221 tattcatatc atagtaaaca cggattgaat cagagatatg tagtaaacca ccattcgctg
     2281 ttaatactgt cttaccatct ttttgtgttg ttggtgcgcc taaactatgt tcaaaagctg
     2341 tccagacgtc tgaacctttt acatcaattt gcgcaatcgt atttccaaat ggtaatactg
     2401 agattaaatc atagcgtgtc accttacctt ttgcgataga ggcacgaata cctccaccat
     2461 ttgtcacggc aaagtcagtc tttttagaga aattcttaac gccatatgct tccatagcat
     2521 ctgcaatcgc gtttcctaaa tttgtttcac gcgttctaac gtcatctctt tctcctttga
     2581 aatcaatcgt attatttgga ataataacct ctgctgtttg tgctctaaat gtttgatcag
     2641 cttgattaat ttgttcagct aatgctttgt tcggtgttac attttcaacg tctttaacat
     2701 taatcaatga cggcttaata ttcgatacct ctccattgcg gtaattaaat gtaatcttac
     2761 cgatattcgc aagtgctgta cctgtttgtg ctaatgcatc attgttataa atttgaccat
     2821 tttgaagtac ggtatgtgaa tgaccatcaa tgactgtaat acgtttcttc aattgtggat
     2881 tttgacttaa ttgtttcact aagtaatcgc cacgccatgt ttcttgtgtt gatggatcaa
     2941 tccctaaatg tgatataaca acaaatgtat ctacgtcttt ataaatacgc atcatttctg
     3001 ctgtcacact ttgtaatgga tctctaaatt caacaccttt aatgccttca ggtcttgttt
     3061 tcgtctttgt ttctggtgtc gttacgccaa taattccata acgaatacca tttttcgtta
     3121 caatcgttga tggtttaaat gcgcgtttcc cgtctttata aacgttagta cttagcatcg
     3181 ggaagtctaa cataccctct aactttttca actggtcgta tccaaagtca aattcgtggt
     3241 tacccactgc catagcatca taacctactg cattcatcgc tttagccatt tcttcacctt
     3301 tagattgatt tgaaagtggt aaaccttgga aggcgtctcc tgcgtctaac attaaatcag
     3361 gcttttcttg ttcttttact gtttttaatt tagccatacc gatgacacgc cctttttctt
     3421 cggctagtcg gccatggata tcatttgtat gtaatatttt atgtgttgtt gtttgtggtg
     3481 cattagcagc aaacattcgt ggtgaaagtg atgctgtttc agcatttgat aatttgaatg
     3541 ttgttgtgtg agttagtttt tgtgttgagg cttgttgtgt atctacgtcg cgcgtttcgt
     3601 ttactgttgt tgaaactgct gtagatggtt tgttagatgg tgcgttgtat gttactttat
     3661 tgtttatttc atcagctgtt ccctgtgctg atgctgatac gttggttgcc gcttcttcac
     3721 cagattgatg agccgcttcc tttgttggcg gtacttgttg cttatctgtt gttgctttgt
     3781 ctatcgttgt tactgcatgt gctttcattg gtgtatgctg ctcagccgcg ctcgagactt
     3841 gccataatcc cattgcacta aaaagcaaaa cgagccatac acttgtttta agtaataaag
     3901 ctttcatttc ggtccctcca ttaactgtca tacaccaata tattatcata atccttatat
     3961 taaaatgatt gattttatgt aaattattta taaattacat ttaatatcga attatttttt
     4021 acatttcaac tagaccctta atttcaacaa cagccatttc ccaccaacaa cataaaaaag
     4081 cacccgataa agtcgtttca cagcagtgcg gaactttatc ggatgactct catttatatt
     4141 gtatatattg gcgttggaat agctttatcc gtatcacata gcaatacttc tttttccgta
     4201 tcaatatcgt gttcgttcaa tacttggcca acactcatac gcgccaaatc tttcatgtta
     4261 ttatcttgtg ataaatgcga taggtaaata cgtttcgtgt tacctgtaat cacgtctgtc
     4321 atcgcatgac ccgcatcctc attagataca tgacccatat cgcctaaaat acgttgtttc
     4381 gtcttccatg gataacgaca cattctcaac atatcgacgt catgattact ctcaaaaata
     4441 aacgcgtcgc tgccacgtat catacctttc atacgatcag acacgtaacc tgtatccgtt
     4501 aaaatcgtaa acttcttata gttattatgg aaaatataaa attgcggatc tattgcatca
     4561 tgtgacacgt taaacgattc aacatcgaaa cctgcaatag attttgtttc ataaggatta
     4621 aaaatgaatt tctgatccat agggatgcga ctatctttct tttcaattgc ctgccaagtc
     4681 ttttcattcg cataaattgg caattgatat ttacgcgcca aaacacctaa tcctttaata
     4741 tgatcaatat gttcatgggt tactaaaata ccatttaaat cttgaatatt gcggtcaatt
     4801 tgactaaaca attcttccat tttcttgcca gtcaaaccaa catcaactaa taaactacct
     4861 ttttcatttt ctacaaaagt ggcgttacct gtactaccac ttgctaatac actcatgcgt
     4921 atcaagcggc tcatcctttc tatttatgca gtcactctct ctaaatgact gcaatataca
     4981 tcagtgcaaa tatacgattt tccctacaga aactatcagt atattagctg ttttgtatac
     5041 ctttcctatt atgtaacttt agttcatcga ccggtctctt ataattaaat tttgataatc
     5101 acgtgcaatg aatgtcctaa ttattataac aatgattaga caaatactaa agtttttaaa
     5161 atagcagtta cgttaatttt acaatgcggt tctacttaaa atatgaataa atgcgcacgt
     5221 tggagacaca aaaacgtcag ccataataaa ccataccaga aaaagctagc accgcttctc
     5281 acatatgatg agcttgcaat gctagcttat tacgattcat attaatgatt aataatttta
     5341 gggttattat ttgtcgcttc gacatagtaa gtattcgttt tgtccttacc gtcatgcttc
     5401 actttaattt cccagtttgg ttgtaacaat tgaacattcg tctcgttcac gactgagtag
     5461 taaccgagtc tagcattaat gacttcatca ccttttttca agtaacgatt ataatataat
     5521 gcctcgattg cttttctcac accaatcact tgatgcttct tatctgaacc atctgtgggc
     5581 ttaatgtcat ccatcattga ttgtttataa ctagtcgctt tgttatcttc tatattaaag
     5641 ttcaacatcg ctttactatt attcaaaatc gggaaattat catacgtttg ttcgtatttt
     5701 acagaaccag aactaatctc acttaattga tattctgaac ctttgaacac acgtttgtta
     5761 acataatctt tcaaatcttt taagttattg tcactgacct ttaccgattg actaatgttc
     5821 gcattcaata attttccacc gtctgatgtg gtcaaatccg aatcgccctt agctttagaa
     5881 ctaaagtctt ttgaacgccc cgtaatttgc tctaatttta tacctttaac tgatttattc
     5941 aatatactag ccggtacttt aatttcttct tgttgaaaat taacttcatt gttactctca
     6001 acctcattaa tgtgtgagcg attgacttta ttaacataaa tcgatactaa cacgatgttg
     6061 acaagaataa acacgaaaat gaaaagtgtc tttgtaagtt tccagttcat ttattcaagc
     6121 ctcccatcgt tataaacata ccattcgcca tcatattcta cataccaacg cggtacgagt
     6181 tcactgttaa tctgcacttc aatgtgatta tgatctgagt tatcctgcat ttcgtaaccg
     6241 atagcgatgt ttgttacttt ttcaaaatta atatcactat tattcgctaa gcttgaacgt
     6301 acagactcta atttcggcaa tgttttatta tcctcactat ttaaaacaac gtcggtgcgc
     6361 aataacgaac gacgataatc aaagacgcct ttttcacacc aagtgacttg aatttgatta
     6421 gagccttctt tattaaacgt tggataaccg ttaaggaaac gttgatatgt taactcgcct
     6481 gactgattat tcgtactaaa caatctgaag tcttcgttta agaaaccacc atgaccatta
     6541 ataaaatcaa aggttcctgg aattgtttct tccattttgc tggaacttgc ttcatcttcg
     6601 gacaagtttt tataatggta tttttcattt ttatcgttat agtttgcgac acctgtatta
     6661 ttgttgtagg ttgtaacacc actctttgaa ctacgaacga tggttgaatc gtcaaatagt
     6721 atagcattca ttttctcaac actaatcgtg ttaaatacca tgcgatatgt ttttaacttt
     6781 tcaggtttac ttggtgcaaa aacatgcgtc gtacgatcaa ttgtatcttt gtttgtgatg
     6841 atatctgtgt atggttgcat atcttttttc actgctgcta atgcatctaa aaagtgatca
     6901 tttttcgttg tagttgttaa ttttacgtaa tcgtggcgat ctttgcttat agcataaagc
     6961 acgatattat catcagcatc atgatctatg accaaacgat tgaaattgaa atgatttggt
     7021 actttcgcat tcatgttcag tacttggcca agatatgttg ataacggtaa atcatacgta
     7081 aaatcgaata atgtaaaatc actgctcaaa tcaggaatca tcaagttatg atcccgacga
     7141 acatgttcca cggacttcac ttctttattt ttcaaaggtt tcgtcagttt attcacatta
     7201 gataccgtcg cgatcgtacc ttctggatga tcatttttcg aatgaataat ctgaaatggc
     7261 gtaatagttg tatccatttt ggctgtcata ggtgtcgtta aaggtttcgt ttcactcttc
     7321 ttactatctg tattgtcgac atttgcaata tcaggagaaa agttccatac catatatgtc
     7381 aatacgacac tcatcaagac gagtagcgct aaaatgacag atttaatatg ttccttatta
     7441 ttcatcccaa tcaccgtctt caatgacttc acatggaagt gtgataaaga tagatgtgcc
     7501 ttgaccttct acactgtttg cccaaatacg accattgtga gcttccacaa tctctttcga
     7561 aatggctagt cctaatccag taccacccat tttacgcgta cgtgccttat ctacacgata
     7621 gaatcggtcg aatatcttat caactttatt gataggaata ccgatgccat tatctttaat
     7681 acgaatcgtc attcgattat aaagtggatt ttgtttcacg tggaactcga cacgtttatc
     7741 acctctagaa tatttcatcg catttgtaat gacattatca aatacttgcg tcattttatc
     7801 aggatcaaat tctgtgaaaa tcgtcttttt cggaatatct cgaataaatg ttgtatcttt
     7861 cgcagacatt tcatgtcgat taataatttt attaatgaac atgttaaagt cgataatttc
     7921 tttattgctt tgatcagact cattatccat tttagataac tgtagcaagt cattgaccag
     7981 tcgaatcatt cgttctgttt cttcacgggt aacagataaa aattgtggcg caagttcctc
     8041 atctttccat gcaccttctt caagtgcttc aatgtaacta ttcatagaag ttaaaggtgt
     8101 acgtaactca tgtgatacat tggcaacaaa ttcacgacgc tcacgttcaa cttgttgttg
     8161 ttcagtaacg tcatgtaaca cagcgatata accagttaca aatcctgttt cctgcacaat
     8221 cgtactaaag ttaacacgtg cgattagacc ttcttcttca tttaaatcta ataagaaact
     8281 atcattattc tcttgaattt cttcaagttt aaattcatct tcaagactta atacacttaa
     8341 catgtaataa ccgatgatgt cttctttcgc cataccaagc atcttaagtg ccatatcatt
     8401 gacgatacga atacgtccac ggcggtctgt tgcaataata ccatcactca tatgggtgat
     8461 aactgagtcc agtctacgtt tctcactttc agtattagcc tgcgcttctt gtacacgttt
     8521 agacaagtta ttaaatgcta aagctaattc gccaatttca tcattaccat aaattttcac
     8581 acgttgcgta tagttacctc tggacatttc gaccgtctgg ttacgcatat cggtgattgg
     8641 tttggtaatc gttcgcgcta taaagaatcc taggatgact gtgattaata atgaaatagc
     8701 tgtaccaaca atgaatattt gatttatatt atttaattgg ttataaacgt cattaatttt
     8761 tgattcgata taaatattac caattacctt tttatcgact ttaacgggga tattatatac
     8821 ccagacacgg tccttaccac cgccataatc ttttaaaatt aaatgatcgt ttgattgtcc
     8881 tagtgatagt gctttttgga cagaactatc attcgctttt tgattgatta gactacggtt
     8941 agactgcttc gtcgtcgcaa taataatttg gtctttatct ataaaacgaa tttctccaat
     9001 ttcttgacgg ttggcatact cacttaataa attttgaata tctttttgtg catttacgga
     9061 gcccttttcg tcatatactt tttcaatact aatttctaat tgtttagcgt actgcgtaat
     9121 attcttctta aaattatcaa gcagctcttt ttcaaggtta tttgtaaaat acagcccgat
     9181 aatttgcata ccaatgataa tcagtaatac ataaacaatt acaagtttag tatgaaggga
     9241 ttgtagttgt tttagccact tcattcgttt cgacctctac tcatgttgtt ggaggaaata
     9301 tccaacgcct ctacgcgtca caatgtattc aggatgtgac ggatcatctt caatcttttc
     9361 acgtaaacga cgaatcgtta catcgaccgt acgtacatcg ccaaagtaat catagcccca
     9421 tactgtttgt aataaatgtt cacgtgtcat tacttgtccc atatgttttg ataaatagtg
     9481 gaacaattca aattcacgat gtgttaattc aatatcttcg ccacgttttt taatagaata
     9541 tgcgtctgga taaatcacaa tatctttaat tgtgatttca ttcgttacat ttccagtgtc
     9601 ttgtgctggt tgtgagtaat gacgacgtaa gttcgctttc acacgtgcga ttaattcacg
     9661 cgtactaaac ggtttcgtta catagtcatc tgcacctagt tctaaaccaa gcactttatc
     9721 aatttctgaa tctttagcag taagcattat aattggcatt tcgtattttt tgcgcacttc
     9781 acgacatact tccataccat cacgaccagg taacatgata tctagtaata cgatgtctgg
     9841 ttcttcttca taaattaagt cgactgcatc attaccatcg tatgcacagt acacatcgta
     9901 tccttctttt tttaagttaa attctaaaat atcagcaatc ggtttttcat catcaactac
     9961 aacaactttt ctagccattt gcataaacct cttttcttaa atcattaata ttattttatc
    10021 attttttgtt ctttatcgtt tatgtttgtg taaaaaatca caggatgtcg cttactattt
    10081 ttgttattgt gcgcattttc cgtcttata
//
LOCUS       DAKSQE010000092        10056 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.092, whole genome shotgun sequence.
ACCESSION   DAKSQE010000092 DAKSQE010000000
VERSION     DAKSQE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10056
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.092"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            99..2174
                     /gene="lip2"
                     /locus_tag="O0V33_002015"
     CDS             99..2174
                     /gene="lip2"
                     /locus_tag="O0V33_002015"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HCX8976637.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVTSHEAQASEKIPTT
                     NAAAQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDMLQSSKHQST
                     QNSKIIRTQNDNQVKQDSERQGSKQSHQNNATNKTERQNDQIQNTHHAERNGSQSTTS
                     QSNDVDKSQPSIPAQKVIPNHDKAAPTSTTPPSNDKTAPKSTKEQDATTDKHPNQQDT
                     HQPAHQIIDAKQDDNVHQSNQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQA
                     PKTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNK
                     YKVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGK
                     TYKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTG
                     GHNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGF
                     KQLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLNGSAKLNNMTSMNPNITYTTYTGVS
                     SHTGPLGYENPDLGTFFLMDTTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFVNVT
                     NDEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATE
                     SKGTQLKAS"
     gene            complement(2416..3243)
                     /locus_tag="O0V33_002016"
     CDS             complement(2416..3243)
                     /locus_tag="O0V33_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCX8976638.1"
                     /translation="MTEIKDKVITKDAFELPYTIIKAKHQPTKGAIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIDDIFTSFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSGINTEPFKMANSYYAKIAQSVNETVLAQLTS
                     PTPIIQDQIAQRFLIYGYARGTGEWINMINIADYTDSKYNISPNELKTLPPVFIAHCN
                     GDYDVPVEESEHIHNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITTA"
     gene            complement(3301..4515)
                     /locus_tag="O0V33_002017"
     CDS             complement(3301..4515)
                     /locus_tag="O0V33_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCX8976639.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNASTKEGYITKADL
                     AYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNIASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLIDWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGEHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLSEQRPHDIDLEFSMADLENLAIPRAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDTSSKDNFS"
     gene            4611..4709
                     /locus_tag="O0V33_002018"
     CDS             4611..4709
                     /locus_tag="O0V33_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976640.1"
                     /translation="MESIFKIKIELMNVICRSENMNMKKKNTSEKI"
     gene            4823..5824
                     /locus_tag="O0V33_002019"
     CDS             4823..5824
                     /locus_tag="O0V33_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCX8976641.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSETSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKYFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEHFPSVDTAQKYKLNDRDKE
                     MIQAHQARIIAGTQEQVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            5858..6190
                     /locus_tag="O0V33_002020"
     CDS             5858..6190
                     /locus_tag="O0V33_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCX8976642.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            6191..6991
                     /locus_tag="O0V33_002021"
     CDS             6191..6991
                     /locus_tag="O0V33_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HCX8976643.1"
                     /translation="METLKSNKARLEYLINDMRRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNRQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKITRGYN
                     LPAKYIIHTVGPQIRRLPVSKLNQDLLAKCYLSCLKLADQQSLNHIAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEAFNRDAE"
     gene            6981..7925
                     /locus_tag="O0V33_002022"
     CDS             6981..7925
                     /locus_tag="O0V33_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HCX8976644.1"
                     /translation="MQSSKWNAMSLLMDDKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEDKQYHIITTNADNAFDAAEYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLQRYNA
                     FLEQHKDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENALYMTMNKKAYRIPNSIQER
                     TIHLTEDISTLITTALRNDSTTQNNNIGETKDVLNRTD"
     gene            7903..8925
                     /locus_tag="O0V33_002023"
     CDS             7903..8925
                     /locus_tag="O0V33_002023"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCX8976645.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQNIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGARDVIQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGID
                     DIKEAKRYELSDADWESIEELASKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIFGDFFGQGDIKDVEQALQGTKMTREDLTHQLKQLDIVYYFGNVTVESL
                     VEMILS"
     gene            9241..>10056
                     /locus_tag="O0V33_002024"
     CDS             9241..>10056
                     /locus_tag="O0V33_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HCX8976646.1"
                     /translation="MNNKVLVTGGTGFVGMRIISRLLEQGYEVQTTIRDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAQHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQASGKRFIATADGQIDLLEIAKLIKEKRPEIAQ"
BASE COUNT         3494 a         1538 c         2069 g         2955 t
ORIGIN      
        1 agtcttaatc aaaaaataat atatgtcatg ttatattgaa agtgcagttg tttttcattc
       61 tcaagagggg atcaaaaaaa tacttttgag gtgattatat gttaagagga caagaagaaa
      121 gaaagtatag tattagaaag tattcaatag gcgtggtgtc agtgttagcg gctacaatgt
      181 ttgttgtgac atcacatgaa gcacaagcct cggaaaaaat accaacgact aatgcagcgg
      241 cacaaaaaga aacactaaat caaccgggag aacaagggaa tgcgataacg tcacatcaaa
      301 tgcagtcagg aaagcaatta gacgatatgc ataaagagaa tggtaaaagt ggaacagtga
      361 cagaaggtaa agatatgctt caatcatcga agcatcaatc aacacaaaat agtaaaataa
      421 tcagaacgca aaatgataat caagtaaagc aagattctga acgacaaggt tctaaacagt
      481 cacatcaaaa taatgcgact aacaaaactg aacgccaaaa tgatcagatt caaaataccc
      541 atcatgctga acgtaatgga tcacaatcga caacgtcaca atcgaatgat gttgataaat
      601 cacaaccatc cattccggca caaaaggtaa taccaaatca tgataaagca gcaccaactt
      661 caacaacacc cccatctaat gataaaactg cacctaaatc aacaaaagaa caagatgcaa
      721 ccacggacaa acatccaaat caacaagata cacatcaacc cgcgcatcaa atcatagatg
      781 caaagcaaga tgataatgtt catcaaagta atcagaaacc acaagttggc gatttaagta
      841 aacatatcga tggtcaaaat tccccggaga aaccgacaga taaaaatact gataataaac
      901 aactaatcaa agatgcgctt caagcgccta aaacacgttc gactacaaat gcagcagcag
      961 atgctaaaaa ggttcgacca cttaaagcga atcaagtaca accacttaac aaatatccag
     1021 ttgtttttgt acatggattt ttaggattag taggcgataa tgcacctgct ttatacccaa
     1081 attattgggg tgggaataaa tataaagtta tcgaagaatt gagaaagcaa ggctataatg
     1141 tacatcaagc aagtgttagt gcatttggta gcaactatga tcgtgctgta gaactttatt
     1201 attatatcaa aggtggtcgc gtagattatg gcgcagcaca tgcagctaaa tacggacatg
     1261 aacgctatgg taagacatat aaaggaatca tgcctaattg ggaacctggt aaaaaggtac
     1321 atcttgtagg gcatagtatg ggtggtcaaa caattcgttt aatggaagag tttttaagaa
     1381 atggtaacaa agaagaaatt gcctatcata aagcgcatgg tggagaaata tcaccattat
     1441 tcactggtgg tcataacaat atggttgcat caatcacaac attagcaaca ccacataatg
     1501 gttcacaagc agctgataag tttggaaata cagaagctgt tagaaaaatc atgttcgctt
     1561 taaatcgatt tatgggtaac aagtattcga atatcgattt aggattaacg caatggggct
     1621 ttaaacaatt accaaatgag agttacattg actatataaa acgcgttagt aaaagcaaaa
     1681 tttggacatc agacgataat gctgcgtatg atttaacgtt aaatggctct gcaaaattga
     1741 acaacatgac aagtatgaat ccaaatatta cgtacacgac ttatactggt gtatcatctc
     1801 atactggtcc attaggttat gaaaatcctg atttaggtac atttttctta atggatacaa
     1861 cgagtagaat tattggtcat gatgcaagag aagaatggcg taaaaatgat ggtgtcgtac
     1921 cagtaatttc gtcattacat ccgtccaatc aaccatttgt taatgttacg aatgatgaac
     1981 ctgccacacg aagaggtatc tggcaagtta aaccaatcat acaaggatgg gatcatgtcg
     2041 attttatcgg tgtagatttc ctggatttca agcgtaaagg tgcagaactc gccaacttct
     2101 atacaggtat tataaatgac ttgttgcgtg ttgaagcgac tgaaagtaaa ggaacacaat
     2161 tgaaagcaag ttaaattcat attcgtaatt taatatgcta tgtattttgt gctactaaca
     2221 tggcacggaa gatataagta gcatcacagt gttgaatttt aaaatagtaa agtgaaataa
     2281 agcgcctgtc tcattagcga aaactaaagg gacaggcgta tctgtttatg agcttaataa
     2341 attgtatgaa taatatggtt gattgaataa ctgtttatca tgatgataaa ttgggttttt
     2401 aaaaataatg gtatattacg ccgttgttat agcgtttaag aaatcaacaa ctttacgata
     2461 aatagtgatt gcttcgtcat taggtctacg atcaaaatca tgctcgtttt tattcacgcg
     2521 ttcaaatgtt gagtgtggca catgattgtg gatgtgttcg ctttcttcaa cagggacatc
     2581 ataatcgcca ttacaatgcg caatgaacac cgggggaagt gttttaagtt catttggtga
     2641 gatgttgtat tttgaatcag tataatcagc aatgttaatc atattgatcc actcacctgt
     2701 accacgtgca taaccgtaga ttaaaaaacg ttgggcgatt tgatcttgaa taatcggtgt
     2761 tggtgaagta agttgagcaa gtactgtttc attcacactt tgagctattt tagcgtaata
     2821 gctatttgcc attttaaacg gttcagtatt gatgccacta taaccataaa aatcaataac
     2881 accatcaata tctctgtctc gtgcaattaa tagacttaaa tatgcacctg atgatctgcc
     2941 aaaggtaaaa atagggcaat tagaatattg tgattgaatc gcatcgaatg atgtgaagat
     3001 gtcatcgatg atgcaatcaa ggcttacttc tggtaataaa cgataactta gttgaattaa
     3061 atcgtaatgt tccgtaagga tatcgatata ctgtggcgat aaatcattgg cctttccaaa
     3121 cattaaaccg ccaccatgaa tgtagacaat ggcacctttt gttggttgat gcttcgcttt
     3181 aataattgtg tagggtaatt caaatgcatc tttagtaatt actttatctt taatttcagt
     3241 cacgactata cagactcctt atttttgaaa ttgatgtcat tataacattg tcttaaattt
     3301 ctatgaaaaa ttatcttttg atgatgtgtc atgataattc tgttctaatt gacgtaaagc
     3361 gtcacgggta tgcttcttta gaccttcccc ataatccatc attttaacaa tatctttaaa
     3421 agcggcacgc ggaatggcta aattttctaa atctgccata gaaaattcaa ggtcaatatc
     3481 atgtggtcgc tgttcagaaa gtttatgcac aaagtcaggt tctgtgacaa aaggcgaaga
     3541 catgccgacc atatctgcat gttgtaaagc atctaaagca gactctggag aattaatccc
     3601 gccacttgca attaaaggga tgcgaccagc taaatgttca tagacaattt ggttaactgg
     3661 ccgaccgaaa tgttcacctg gtgtacgaga cgtattttga taaatatgtc gaccccagct
     3721 agcgattgct aaatattgga tgtttgaaac gtccatgacc caatcgatta attgattgaa
     3781 ctcgtcaatg gtatatccta aatcactacc tctggtttct tctggcgttg ctcgaaatcc
     3841 taaaataaaa ttgtcaggtg cttctttatc aatcacttct tgtaccgcac gcataacttc
     3901 taaacataat cttgcacgat tttttaatga gtcggcaccg taatggtctg tacgtttatt
     3961 cgaaaaagtt gagaaaaatg tttgaatcag caaacgttgt gcaatcgaaa tttccacacc
     4021 atcaaaacct gctttaatcg cgcgtaatgt agcatcgcga tactgctgaa tgatgctatt
     4081 gattttctca tgagacatgg cgataacatc gtgttcaatc ggtgaatgca acgtcatagg
     4141 acttggtcca tacacctttc caaaatttaa aattgcttga tttgaaaaac gaccagcatg
     4201 cgctagctga ataatagcga ggctaccatg ttgtttcatc gtagatgcta tgttagttaa
     4261 tccagggata caagcatcat gatcaatatt aaagccgtat tcaaacaatt gaccataagg
     4321 ttcaatgtaa gcagcgccgg tgacttgcat accagctgaa ttagagcgac gtgcagcata
     4381 agccaagtct gcttttgtaa tatagccttc ttttgttgat gcgtttacgg tcattggtga
     4441 taatacaaag cgattcgaaa ttttgatgcc attaggtaag tggattgatt gtaaaagtgg
     4501 tttgtatcgg tacatactat gattcctttt ctattcaata ttgttttcaa agtaccatgg
     4561 aaagaatgaa taatcaatga tgaacagtct tgatagaata gaattggtac atggaaagca
     4621 tttttaaaat taaaattgaa ctaatgaatg tcatttgtag gtctgaaaat atgaatatga
     4681 aaaagaaaaa tacaagcgaa aagatataaa agttaattga aaaacgttat catatacgtg
     4741 ggtatatgaa gagggaatgg tattaagaac gttaaaatgt tatgtcggtt tgatatgaca
     4801 ggataagttt ggagatgacg gattggttaa attaagcgta ttagactatg ccttaataga
     4861 tgaaggtaag gatgcacaaa aggcgttgca ggattcagtg acacttgcaa aattagcaga
     4921 tcgacttggc tttaagcgaa tttggtttac agaacatcat aatgtaccag cgtttgcgtg
     4981 tagtagtcca gaacttttga tgatgcatac attggcgcag acaaatcaca tacgagttgg
     5041 ttctggtggt gtgatgctgc cgcactatcg accttataaa attgctgagc attttagaat
     5101 gatggcagcg ttatatccaa atcgtattga tttaggtatt ggtaataatc cgggtactac
     5161 tatggtaaag caagctttag atggaataaa tcctacatat gatagttacg atgaatcgat
     5221 ttcgttatta cgtgattatc ttacaataaa ggataagcca agtgcgcata cgttaggtgt
     5281 ccaaccacac attgatcatt ttccagaaat gtggttatta agtagtagcg aaacatctgc
     5341 caaaatagct gccgaactag gtatagggct ttctgttgga acatttttgc taccagatat
     5401 aaatgcgata catgcagcga aggataacat tgatatttac aaaaaatatt tccaagcatc
     5461 aacgattaaa atggacgcaa aggtgatggc atctgtattt gtcattgtag ctgataacga
     5521 agcggaagta gcagcattac aacatgcctt agatgtttgg ttattaggta aattacaatt
     5581 tgcagaattt gaacattttc catcggtaga cactgcgcaa aagtataagc tcaatgatcg
     5641 agacaaagag atgattcaag cacatcaagc acgtatcatt gcaggtacac aagaacaggt
     5701 taaagcacaa ttagatgatt tcattgctac gtttgaagtt gatgaggtgt tagtagcacc
     5761 gcttattcca ggcattgaac agcgttgtaa aacattaaaa ttactcgcgg aaatctattt
     5821 gtagcatttt aaatagaaga gaaaggatga agataagatg aaaaagttag ccaattattt
     5881 atgggtagaa aaagtaggag atttgtatgt gtttagtatg acacctgaat tgcaagatga
     5941 tattgggaca gtaggttatg ttgaattcgt gagtccagat gaagttaaag tggatgatga
     6001 aattgtgagt atcgaagcat cgaaaacggt cattgatgtg caaacgccat tgtcaggaac
     6061 gattattgag cgaaatacaa aagcggaaga agaaccgaca attttaaact ctgaaaaacc
     6121 agaagaaaat tggttgttca aattggatga tgtcgataaa gaagcattcc tggcattacc
     6181 ggaggcttaa atggaaacgt taaaatcaaa taaagcgaga cttgaatatt taatcaatga
     6241 tatgcgtcga gagagaaatg acaatgacgt attggtaatg ccatcttcat ttgaagattt
     6301 gtgggaatta tatcgaggtt tagcaaatgt caggccggca ttacctgtaa gtgatgaata
     6361 tttagctgta caagatgcta tgttaagtga tttgaatcgt caacatgtta cggatttgaa
     6421 agatttgaag ccaataaaag gtgacaatat ctttgtttgg caaggtgata tcacgacgtt
     6481 aaaaatcgat gcaattgtta atgctgcgaa tagtcgcttt ctaggatgta tgcaagctaa
     6541 tcatgactgc attgataata ttattcatac aaaagcgggt gttcaagttc gacttgattg
     6601 tgcagagatt attcgacaac aagggcgcaa tgaaggtgta ggtaaagcca aaataacacg
     6661 tggatataat ttgccagcaa agtatataat tcatacggtt ggtccgcaaa tacgtcgatt
     6721 gcctgtttca aagttgaatc aggacttgtt agctaaatgc tatttgagtt gtcttaaatt
     6781 ggctgatcaa caaagtttaa atcatatagc attttgctgt atatctacag gtgtatttgc
     6841 ttttcctcaa gatgaagcag cagaaattgc tgttcgaaca gtagaaagct atctcaaaga
     6901 aacaaattca acattgaaag tcgtgttcaa tgtatttaca gataaggatt tacaactata
     6961 taaggaggca tttaaccgtg atgcagagta gtaaatggaa tgcaatgtct cttttaatgg
     7021 atgacaagac aaagcaggct gaagtattgc gtactgcgat tgatgaagca gatgcgatag
     7081 tgattggaat tggtgcaggc atgtctgcat ctgacgggtt tacatatgta ggagagcgtt
     7141 ttacggaaaa tttcccagat tttattgaaa aatatcgctt ctttgatatg ttgcaagcga
     7201 gtttacatcc ttatggcagt tggcaagagt attgggcatt tgagagtcgt tttattacat
     7261 taaactattt agatcaacct gtaggtcagt cttatctcgc attaaaatcc ttagtggaag
     7321 ataaacagta ccatatcata actacgaatg cagacaatgc tttcgatgct gctgaatatg
     7381 atatgacgca tgtatttcat atacaaggcg agtatatact acaacagtgt agtcagcatt
     7441 gtcatgctca aacgtatcgc aatgatgatt taattcgtaa aatggttgtt gcgcaacaag
     7501 atatgcttat accttgggag atgattccaa gatgtcccaa atgtgatgcc ccaatggaag
     7561 tgaataaacg taaagcggaa gttggcatgg ttgaagatgc tgaatttcat gcgcaactac
     7621 aacgttataa tgcttttcta gagcaacata aagatgataa agtgttgtat ttggaaattg
     7681 gaattggtta tactacgcca caatttgtga aacatccttt tcagcgtatg acacgtaaaa
     7741 atgaaaatgc cctttatatg acgatgaata aaaaggcata tcgcattccg aattcaattc
     7801 aagaacgtac catacattta actgaggata tctcaacatt gattacaaca gcactccgga
     7861 acgatagcac aacgcaaaat aacaacattg gagagacaaa agatgtactt aatagaaccg
     7921 attaggaatg gagaatatat tactgatggt gcgattgcac tcgctatgca agtttatgtt
     7981 aaccagaata tctttttaga tgaagatatt ttattccctt attattgtga tccaaaagtg
     8041 gaaattggac gttttcaaaa tactgctata gaagtgaatc aagattatat agataaacac
     8101 agtattcaag tagttcgccg agatactggt ggtggcgctg tgtatgttga taaaggtgcc
     8161 gttaatatgt gttgtatttt agaacaagac acttcaattt atggtgattt tcaacgattt
     8221 tatcaaccag ctataaaggc gttgcataca ttaggtgcaa gagatgtgat acaaagcggt
     8281 agaaatgatt taacattgaa cggcaaaaaa gtgtcaggcg ccgccatgac attaatgaac
     8341 aatcgtattt atggcggtta ttcgctatta cttgatgtta attatgaagc aatggataaa
     8401 gtgttaaagc ctaatcgcaa aaagattgca tcgaaaggga ttaaatctgt gcgtgcacgt
     8461 gttggtcatc ttagagaagc actggatgaa aagtataggg atataaccat tgaagaattt
     8521 aaaaatttaa tggtgacgca gattttggga atcgatgaca ttaaagaggc gaaacgatat
     8581 gaattatctg atgcagattg ggaatcgatt gaagaattgg cgagtaaaaa gtataaaaat
     8641 tgggattgga attatgggaa gtcacctaaa tatgaatata atcgaagtga aagattatca
     8701 tcaggtactg tagatattac catttctgtt gaacaaaatc gtattgcaga ttgtagaatt
     8761 tttggtgatt tctttggaca aggtgatata aaagatgtgg aacaagcatt acaaggaaca
     8821 aaaatgacaa gagaagattt aacgcatcag ttaaagcaat tagacatcgt ttattatttt
     8881 ggtaatgtta cggttgaatc attggttgag atgattttaa gttaatattg tttaggtatt
     8941 aatgtcgcac tctcgttgaa catgtgtatt tgcttttaaa atggtgacta tgtagttatg
     9001 tgatttgaac atacgattga aaatgaattt agttaattag caaagacatc atatttatag
     9061 cgtttgtgtt tattagtgat tgggtatttg aaaatggtta atgatagttt ttaaaggaat
     9121 aaattcattt tcatttacac aagtaagatt aaataatagc taccaaagtc tatttatata
     9181 cgtcaaaata taatgactgc tataatgaat aatcaataga cacaaagagg agattatgtg
     9241 atgaataata aagtattagt gaccggtggt actggttttg ttggcatgcg aattatttca
     9301 cgattattag aacaaggtta tgaggtacaa acgacgatac gtgatttaag taaagctgat
     9361 aaagtaatta aaacgatgca agacaatggc atttccacag agcgattaat gtttgtcgaa
     9421 gcggatttat cacaagatga acattgggat gaagcaatga aagattgtaa atatgtgttg
     9481 agtgtggcat ctccggtgtt tttcggtaaa acagacgatg cagaagtgat ggcgaagcct
     9541 gcaattgaag gtatacaacg tattttaaga gctgcacaac atgcgggtgt taaacgtgtg
     9601 gtaatgactg caaactttgg tgcagttggt tttagtaata aagataaaaa ttcaatcaca
     9661 aatgaaagtc attggacaaa tgaagatgaa ccaggcttat cagtatatga aaaatcaaaa
     9721 ttgttagctg aaaaagcagc gtgggatttt gttgagaatg aaaatacgac agtagaattt
     9781 gccacaatca atccagttgc aatttttgga ccatcattag atgcacatgt ttcaggaagc
     9841 tttcatttat tagaaaattt attgaatggt tcaatgaaac gtgtacctca aattccatta
     9901 aatgttgttg atgtgagaga tgtagcagaa ctacacattt tggcaatgac caatgagcaa
     9961 gccagcggta aacgatttat tgcgacggct gatgggcaaa ttgatttatt ggaaattgca
    10021 aaattaatta aagaaaaacg acctgaaata gctcaa
//
LOCUS       DAKSQE010000093        10043 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.093, whole genome shotgun sequence.
ACCESSION   DAKSQE010000093 DAKSQE010000000
VERSION     DAKSQE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10043
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.093"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(361..1515)
                     /locus_tag="O0V33_002025"
     CDS             complement(361..1515)
                     /locus_tag="O0V33_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCX8976647.1"
                     /translation="MKLSLNSNSKFLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPIFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPNIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
     gene            complement(1710..2720)
                     /locus_tag="O0V33_002026"
     CDS             complement(1710..2720)
                     /locus_tag="O0V33_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCX8976648.1"
                     /translation="MTSLKERDYFFDNARAILILLVVFGHMLQPYTSGDKYLSALYLV
                     IYSFHMPTFLFISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFLTGKSDELQ
                     LDPFNPVFALWFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSYLSIS
                     RTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIIYVIHPINADWLLGSSP
                     YTSLENEGQSVFSPFKRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTLYVYLLHGL
                     IIGIVRGFEWYPFDNPISLMTYLYLISISVIIVYVLSTNFVCKWTNPIINLQRPSQFK
                     DS"
     gene            2873..3292
                     /locus_tag="O0V33_002027"
     CDS             2873..3292
                     /locus_tag="O0V33_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCX8976649.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDITSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            3319..3429
                     /locus_tag="O0V33_002028"
     CDS             3319..3429
                     /locus_tag="O0V33_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976650.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(3500..7273)
                     /locus_tag="O0V33_002029"
     CDS             complement(3500..7273)
                     /locus_tag="O0V33_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional autolysin"
                     /protein_id="HCX8976651.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKAANKTGNAQVNQKVDTTQVNG
                     DTRATQSTTSNNAKPVTKSTNTTAPKTNNNVTSAGYSLVDDEDDNSENQINPELIKSA
                     AKPAALETQYKAAAPKATPVAPKAKTEATPKVTTFSASAQPRSAAAAPKTSLPKYKPQ
                     VNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTINGEI
                     SYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASFAR
                     SMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSHNY
                     SYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPSTPSKPSTPSTGKLTVAANNG
                     VAQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWV
                     KEGDVVYNTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQ
                     IDKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQIN
                     AKNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDV
                     IYNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSI
                     YLYGTVNGKSGWISKAYLAVPAAPKKAVAQPKTAVKAYAVTKPQTTQTVSKIAQVKPN
                     NTGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLN
                     VQNLGKEVKTTQKYTVNRSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDV
                     YLYGTINNRTGWVNSKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKY
                     SLKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQIGMTLNQV
                     AQIQAGLQYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISI
                     DKINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNN
                     KVVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAG
                     QNTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
     gene            complement(7501..7935)
                     /locus_tag="O0V33_002030"
     CDS             complement(7501..7935)
                     /locus_tag="O0V33_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCX8976652.1"
                     /translation="MFSKVIDPEMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVIKSHRGQGMGKMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSHN"
     gene            complement(8091..8561)
                     /locus_tag="O0V33_002031"
     CDS             complement(8091..8561)
                     /locus_tag="O0V33_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HCX8976653.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIENPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(8609..9835)
                     /locus_tag="O0V33_002032"
     CDS             complement(8609..9835)
                     /locus_tag="O0V33_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HCX8976654.1"
                     /translation="MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNA
                     NRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQI
                     RMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFV
                     EAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRG
                     QRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIK
                     TAQLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYDSL
                     IPLTPLDSSLLRKEQNDTTDKDKSSNNTDDSNNSNNNNSVNNQQQPATDQNLNQNQGG
                     TQQTPQASNNQNGVVN"
BASE COUNT         3179 a         1548 c         1627 g         3689 t
ORIGIN      
        1 ttggtaaggt cgtcaattgt ttgcatttat ctaaaagaat aaataagaaa ggaattgaga
       61 gcgaagagaa agtatttgtt tataataata aatagtaacg tttgaataat aattgtttgg
      121 tcaaagatag attaatatga atgctgatat attatttgag tgcttaagtt tcgaagaaca
      181 attatcagtt agcatattaa aagcttttga tagaatcatt ttgagtgagg tgtttaaagg
      241 agaggaatat aactcggtag tatttacagt ctctttagag tattatttaa aacatataga
      301 atataaaaaa ctgaacgtac tatgaatttc gttcacgacg tccagttcaa catatcattt
      361 ttatttagta tttaaatatt tagcaagtct cttcatacct tcttttaaaa catccatttc
      421 ataagcatag gaaatcctca caaatccttt accgaattct gtaaaggatg agcctggaac
      481 gattgctaaa tgtgttgatt caagtaaatc gacacaaaat tcgaaatcgt catcggtgat
      541 atgcttaata tttgggaaaa tataaaacgc gccttcaggt tgagcggtaa tctcaaaacc
      601 taatttagtt aattcagata ctaaataatt tcttcgttct acataagctt cattcatata
      661 tttaggagct tctaatcctt cgttaagtgc tgttatacat gctatttgag ctggaacatt
      721 ggcacaaata caattatagg cgtgcataaa tgttaattta tcaatcaaat attgaggtcc
      781 taatagaaaa ccaattctta ttcctgttgc tgagtgtgat ttacttaaac caccaattaa
      841 tattaattga tcacgaatgt cttcaaattc agcgaaggat acatgtttac cactaaatgt
      901 attttcagca taaatctcat cgctaataat aaatatcgga tattttttta atacatttac
      961 gatatttaaa acttcatttc tttttaaaac tacgccagtt ggattagtcg gataatttaa
     1021 caagacagct tttgtctttg gagaaatatg actttctaac gcatcaggtg taattttgaa
     1081 ttgtgttgct gttgtatcaa tataaattgg tttaccacct agtacttcga tgagtggtat
     1141 gtagcctgca taaattggtc ccggtattat aatttcatct ccaggctcta tgatagaacg
     1201 taacgttgtg tctattgctt cacttgctcc atttgtcaca ataatttctt caggatcata
     1261 ggaaaaatga taacgatttt tgaagtattg actaatagct tcgcgagttt ctaataagcc
     1321 tttattgtga gagtaagatg tcttgtcatt gttaatagca tctatatatg cctttttcac
     1381 aacatcaggc atagggaagt ctggttggcc aatagttaaa ttaacacaat catccaaatt
     1441 attcatacga tttgaaaatt ggcgaatact tggtgctctt aaaaatttag aattagagtt
     1501 taaagaaagt ttcatcgtga cacctcaaat ataaatcaaa tattgtctaa aaatttagaa
     1561 attaataata tcataacata cttttaaagc aaaaaagggt aaattactta agctttattt
     1621 gaaaaatgaa aaatgtctaa atacactgta gtaactactt ttcaaaatga atgaagtggt
     1681 tacagttaat gtacttagac attatataat tatgagtctt taaactgtga tggtctttgt
     1741 aaattaatta ttggatttgt ccatttacaa acaaagtttg tagataatac atatacgatg
     1801 attacagata tacttattaa ataaagatac gtcattaatg atataggatt atcgaatggg
     1861 taccattcaa atcctctaac aatgccaata attaaaccat gtaataaata tacgtatagc
     1921 gtacgactgc caatataagt atataatttt ttctttgttg acattaaatt tagaaacgca
     1981 gtcattgcga ttaatataat tccatataat ataagtcgtt taaaaggact gaatacactc
     2041 tgtccttcat tttcaagtga agtatatggt gaacttccta ataaccaatc tgcattgata
     2101 ggatgaatca cgtaaatgat aaaaaataaa ataaacgtaa tgatagatac tggtattagt
     2161 tttttatttt taaaaatagc cgtatgtttt ttggtgaaaa tgtagcctag gtaaaatatt
     2221 gggaaaaata cgattgtcct tgaaatgctt aagtagctat cgatgttatc tgaaaaacct
     2281 gctccaatag atataataat tgaaactgat agcactttat atggattaaa tcttctaact
     2341 attactaaaa tgacatgaaa gaaaaatagc gtgatcaaaa accataacgc aaatactggg
     2401 ttaaaaggat caagttgtaa ttcgtcactt ttacctgtta agaaataata tattgaaaag
     2461 aatgcaaaaa atatcatata aggtactatc aagcgttttg aaattttttc taaatagtat
     2521 ggtttatcaa tatttttcgc gaaataacca gatataaaca aaaatgttgg catatggaaa
     2581 ctataaataa ctaggtataa tgctgataaa tatttatcgc cactagtgta gggttgtaac
     2641 atatgtccaa atacgactaa taatattaaa attgctcttg cgttgtcaaa aaaataatct
     2701 ctctctttta atgaagtcat attttatctc ctttatgtcc ataagactct tactaattta
     2761 ttatattttg tattagttat gcaacaaatt tgtctattgt gtcgaatagt attgtaaaaa
     2821 ttataaaaaa agcatataat attataaata atatgtagtg gagtagaaca acatgtataa
     2881 acaacttgaa aaacttatta cactgactaa caatgactta aacttagtga atagaagatt
     2941 tggacaacgc acggatatca catctgaaca gctagaactt ctccgtattt tatttaatta
     3001 cgatcgctta tcacagtatg atttgacgat gaagattagt agggaacaat ctatagtttc
     3061 aaggtggatt aagaaattag ttttgaaagg gtacatcaca agtcaacaat ctagcgaaga
     3121 tttaagatgt aaagaattaa ttttgactga tcaagcacgt acattaattt cacaaataaa
     3181 taatgcacgt tgcgaattga ttgaagcaag atgtcaatgt ttatcggaag tcgaattaga
     3241 caatttaaat caattacttg ataagttaaa tcaacgacgc atatcgttgt aacacggcaa
     3301 tagcaaacac ataatttagt gtcattgtgc acacaacaaa aattgaatca tgaattacaa
     3361 gcaaaagtag cggtgattgt taaaattgat ggtaaacaat caccgctatt tttgcttgtg
     3421 tatgtataaa aaagggatca aaggtcatcc cccatgattg atagtggggg gatgactttt
     3481 gatcctatgt tcatgttgct tatttatatt gtgggatgtc gaagtatttg ccgacttcgc
     3541 caattttatc atagtagcct ttgatgattt tagcattgat gttagcccaa tctacatctg
     3601 tagcatattg gtgtgttcct ggatgtgcag gattccatct cattttgtaa agcgtatttt
     3661 gaccagcttt aacatatgag ttgccgatga atttagcacc accaacgatt gcttttgata
     3721 ctgtgtccca accagcttgt ttagcatatt taataccttc acgtaaagga tcgttatcat
     3781 atgcagcaat accaaatacg ttatggtatt tcgtgttaga gttagttaca actttgttgt
     3841 tcactacatc tgcacctttt gctaattgag aagtaccgtt acctgtttct aatagggcat
     3901 gtgagataag ataaacttca ttaatgccat acatttgagc agctttgtta aatgcagcac
     3961 cttggttttc taatacacct ttacctttta agaattgatt aattttatca atagaaatat
     4021 tttgtggttg gtctaagcgt aagaattgat attttaatgc tggatcttga gctaaacgct
     4081 tcgtatccat tgcatgctta acatcattaa agttagcatc tgtccactta cctggtacac
     4141 gttgtacttg tggtttatat tgtaaaccag cttgtatttg agcaacttgg tttaatgtca
     4201 tacctatttg attatactta attaattctt tagctaaatc agttgattta atccatgcta
     4261 atttaccgtt agataattta ccatagtacc aagtttgtcc attaatgact tgttctttaa
     4321 caactgcgaa tggttgttca ttaaatgctt ttaatgagta tttagctgta tcagaatttg
     4381 gtgttacata gtaataacca ttaccatttt taattacata agtgtagtta taatctttgg
     4441 cagctgatgt agttggttta acagcagttg gtgcagttaa atcttttgaa tttacccaac
     4501 cagtgcggtt attaatagta ccgtataaat aaacatcttt gcctacagat acttgtttcg
     4561 ttgcattaaa tgtaccttga gcaatgttat tgcctgttaa aatgacttgg tttttagtac
     4621 cccaaggaac cattgataag ccgttatttg atctgttaac agtatatttt tgagtcgttt
     4681 taacttcttt gcctaagttt tgaacattta agtcttttac attgaaccaa cctaatggaa
     4741 tattatgact tgtattattt aataatacgt atgtttcatt accatgtgca cgttcttttg
     4801 ttacatagaa tgtacgatcc gcatattttg caccgttttt cgctgttttt tcataaacag
     4861 aagcacgaat accagtgttg tttggtttaa cttgagcaat tttgctaact gtttgagtcg
     4921 tttgaggttt agtaacagca taagctttta cagcagtttt tggttgtgct acagcttttt
     4981 taggtgcagc aggtacagct aaatatgctt tacttatcca accagattta ccatttacag
     5041 ttccatataa atagatagat ttatcaattt gttgttgctt agtcgcttta aaagtttggt
     5101 tacctgtacc agataccgca ccagcttctt gtttataagt accccaaggt actgaatata
     5161 atttagtgcc tggttttact gtataagttt gcattacatt tacaggtgat tttgcattgt
     5221 tataaataac gtcaccttgt ttaacccaac caattaaagt tggactattg taatctttaa
     5281 ctaagtagaa tttgtttcca cctagacttg cttcttttgt tacagcaaat gttttttgaa
     5341 cttctttcgt tggcttacca gttttgtcat aaactgtagt gaataagcca ttgtttttag
     5401 cattaatttg agcaacaccg tttaatgatg aaactgttaa tttattattt gttgtaggtg
     5461 ttgatggctt aggtgttggt gtaggcgtag gtttagcagt atcaactaaa tatgctttac
     5521 ttacccaacc agatttacca ttcacagagc catataaata aattgattta tcaatttgtt
     5581 gttgctttga agccttaaat gtttggtttc cagaaccaga cacgctacca gcaacttgtt
     5641 tagatgtacc ccaaggtact gtataaagtt tcgtaccagg tttgattgaa tatgattgat
     5701 ttacatttac aggtgattta gctgtgttgt aaaccacatc gccttcttta acccaaccaa
     5761 atttattacc agaattgtaa tcttgaacaa gatagaattt ttgattacct aatgtagctg
     5821 ttttagatac agcaaatgtt ttttgaactt cattagttgc tttaccagtt ttgtcgtaaa
     5881 cagtagtata taaaccacta tttgtaggtt tgatttgtgc gacaccatta ttagcagcaa
     5941 ctgttaattt accagttgat ggtgttgatg gtttcgacgg tgttgatggt tttgatggtg
     6001 tagtaggggt agttgtagat tgcgtacccc atggcgccac tttacccatt tttattaaat
     6061 atttttcatt aattaagtca tatagttgat cataactata attatgactt cttaaatatc
     6121 catgtggatc ggcatggtcc gtaccaccta aatatttact tacagcgtag tgagtccata
     6181 ctgtaccatt tccatcatat tcagcactat caggttttaa accataatat tgtaattgtg
     6241 tagctgcata gtcagcatag ttattcattg aacgtgcaaa tgaagcataa tcgtgtgtgt
     6301 gcacgatttc aacattgatg aatctagggt taccgactgc accgacaccc caagataagt
     6361 aatccgttgg tgctgtttcg attatacgat ccccatcaac aaatgcatgt acgaatgcgt
     6421 tttgatagtt gtttttcata taactaattt cgccatttat cgtcgaacga tcattagctg
     6481 tatcatgaac aacgatacct tcaggacgac ctacaccgtt acggtatgcg tatttaggga
     6541 agtaagatgt ataatcttcc tctatcttag gtgcttttaa attattttta cgaatgtaat
     6601 cattaattga tgagtttact tgcggtttat attttggcaa actcgtttta ggtgctgcag
     6661 cggctgatct tggttgtgct gaagcactaa aagtagttac ttttggtgta gcttcagttt
     6721 tagctttagg tgcaacaggt gttgcttttg gtgctgcggc tttatattgc gtttcaagag
     6781 cagcaggttt agcagctgat ttaattaatt ctggattaat ttgattttct gaattatcat
     6841 cttcatcatc aactaaacta tatccagcac ttgtaacatt attgttcgtt ttaggtgctg
     6901 ttgtgtttgt tgactttgta acaggtttcg cattatttga tgtagttgat tgagtcgcac
     6961 gagtgtcacc atttacttgt gtagtatcaa ccttttgatt tacttgagca ttgcctgttt
     7021 tattcgctgc ttttggttgg acaatagcag ggtcttgata tacttgagtg ccagaaatgt
     7081 tttgcgttgg attttttacc tcagcttttg cttgttcagt agttgcttta actttattac
     7141 tatctaaaac atttttatta gtagtttgat cttgtgtcgt ctcagctgct tgaacttgat
     7201 gtgcagtgac tgctgaacct acaagcgtta atgcaaccat tgatggtagt ttgtaattaa
     7261 attttttcgc cattctattt attactccta acatttatta attattaatc tcattatagt
     7321 acctgtttta tacacctgtg cgtatttaac caatttgtaa tatactctta atttatattt
     7381 tcactgtaaa aaaatgaaaa aaattttgaa tctcaatggt gacgtgaaaa atcaaataga
     7441 attgatgtca atttaagaat tggtttatta caaatgtttc tataatagat acaacttttt
     7501 ttaattatgc gaggtcaact tttttgtcat ttcaatatgc tcgatgcctt cctcaagaaa
     7561 tatattacct ctcattttaa aatttaaact ttcatagaat gggattgcat gacattgggc
     7621 attcatggta gctacgtaaa aaccttcatc tttagctaat gattctacag cttgcataag
     7681 cattttaccc atgccttgtc cgcgatgtga ttttatcaca gccactcgtt ctattttgac
     7741 agttgtttca ttgataggac gtatacgagc tgtggcaact ggctgtccat tatcatatcc
     7801 aatgaggtga atagattcag attcatattc atcaatttca ctttcctctg ggacgccttg
     7861 ttcttctaca aacactttct ttcttatata aaagcaatct tctaacatct ctggatcaat
     7921 cacttttgaa aacataaaat ccctccatat cctaacaaaa tcatttacat ttaatttact
     7981 aataataaca tagaataacg attgttattt tccacaaaaa taattagaca taaaaaagct
     8041 ataaagatga ttcataggaa ccatctttat agctagatag agtgtatttg ttataatgct
     8101 tcttttctaa taattgctgt atattgccaa aagattcgca tttgagcaat attccaatta
     8161 ttcattccca ttgtaaaccc agaaggttta aagatattta catctgtcac ttctaaagct
     8221 gctgcgacta aatattgtaa tggaatacta agtgatttca ttgtttcagt aatgccattt
     8281 tcatcatata cccatgaaaa tggtaactct gattcgaaaa catcaacact attgaaaatt
     8341 tcaggtaggg ctaaaatata acatgcacca tctacaatag gattctcgat actatttttg
     8401 taataaatta ggagtgcttc atagttttct cgatgctcat gattgacgta aaaaaattca
     8461 cttctaaaat gagccatatc ttggctatga ataatttgtt gttctaacat attaaacatc
     8521 ccatttatat ttgcaatctt ttcgtatgtt tttctactca tgtatgatgc tcctttcata
     8581 aatagtaaat gttaatcgtc atataatatt agtttacaac accattttgg ttatttgaag
     8641 cttgtggcgt ttgttgagtg ccaccttgat tttgatttaa gttttgatct gtagcaggtt
     8701 gttgttgatt attgacgcta ttgttgttat tactattatt actgtcatca gtattattcg
     8761 aagatttatc tttatcagtg gtatcgtttt gttcttttct taataaacta ctatctaaag
     8821 gtgttaaagg tatgagagaa tcataatgat taatgacacg ttgatctaag aaatcatttt
     8881 tatcattaat aggtgataat tctaagtctt tacgaagtaa gtttgcatat ttttgaatgc
     8941 tttctacact tggatgataa tagtaaatac catttaacat atcatcttta ccttttaatt
     9001 gcgcagtttt aatttcaaca tcatttgtta agtacatttt tgctaaagct ttaatttcag
     9061 agttagttaa attatgcttt gcatttttac ctacaatttg aatcacgtta tcaagtttat
     9121 caatagaatc aacttcctgt gctttttgga ataaaatctt aattaattcc atttgacgtt
     9181 gtccacgttt caagtctgaa tcatgatgtc tagttctagc aactgctaaa gcctcatcac
     9241 catttaattt ttggtaccct tttttaattt taatcttacc agtatcatct gtgtttggct
     9301 catttaaatc atatggtaca tcatagtata tgccaccaag ttcatttaca gcttctacaa
     9361 aggccttcat attaacgcgt acatagtaat caacaggaac attcatcgtt gcttcaacag
     9421 agtccattgc tgcaattgga ccaccatatg catgtgcatg ggtaatctta tcgtaatagc
     9481 cgactttagg aatgtagctg attgtatcac gaggaatact aagcattcga atttggtgtt
     9541 tagattgatt aaaagtagtt aaaatcatag cgtctgatct agagtgttca gcatcttggc
     9601 ctttttttct tcttccgtca ttatcatcga tacctaagaa gagaatagag ataggttgtt
     9661 cttcaggatt gactttattg tctcgtaaat ttgattgacg attagcattt ttactatctt
     9721 gagaagattc aaatgcatct tgagacgttt taaaaagtaa cgtagcgaag acaattggaa
     9781 caacaataag aaccaatgct agaaggatca aaaagtattt taaaaattta ttcatgattg
     9841 atgctcctat attaaatttt tgttaaatca taatcataat tttaaaatat aattagttga
     9901 acgatattta tacaacttca ttactaatac attatagcct taaattgtag tactttatag
     9961 taaaaaatac aaatgttatg tcaaagttcc ttgtaatttg aatgttttgt taaaatcaaa
    10021 aagcatttta aaaaatataa tgc
//
LOCUS       DAKSQE010000094         9382 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.094, whole genome shotgun sequence.
ACCESSION   DAKSQE010000094 DAKSQE010000000
VERSION     DAKSQE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9382
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.094"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            93..239
                     /locus_tag="O0V33_002033"
     CDS             93..239
                     /locus_tag="O0V33_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976655.1"
                     /translation="MMLTAIYFSIFTFYISQYSLKLKTLYNIETDYNYKIVLLLKEGK
                     THES"
     gene            229..753
                     /locus_tag="O0V33_002034"
     CDS             229..753
                     /locus_tag="O0V33_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCX8976656.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKY
                     KLTIPEIFSKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEVFNFLKNQKNIIW
                     LDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRILRYNEIAFKKFDSHLLSISEI
                     YYELLNLIKASDQY"
     regulatory      740..837
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            912..2003
                     /gene="gcvT"
                     /locus_tag="O0V33_002035"
     CDS             912..2003
                     /gene="gcvT"
                     /locus_tag="O0V33_002035"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCX8976657.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLV
                     IYKLADDNYLLVVNAANTEKDFNRILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLIN
                     QLVDEDVTEMKMFEFKQGVKLFGAIVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEY
                     NVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQ
                     KENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRD
                     EFEMGRELLVQVRKRQLKAKIVKKNQIDK"
     gene            2023..3369
                     /gene="gcvPA"
                     /locus_tag="O0V33_002036"
     CDS             2023..3369
                     /gene="gcvPA"
                     /locus_tag="O0V33_002036"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HCX8976658.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AESEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDDTAAVAVQYPNFY
                     GSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            3362..4834
                     /gene="gcvPB"
                     /locus_tag="O0V33_002037"
     CDS             3362..4834
                     /gene="gcvPB"
                     /locus_tag="O0V33_002037"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HCX8976659.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPISDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSEHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPYTTVIDRLDEATAARKPILKFENLKQEK"
     gene            complement(5198..5584)
                     /locus_tag="O0V33_002038"
     CDS             complement(5198..5584)
                     /locus_tag="O0V33_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCX8976660.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKSKK"
     gene            5742..6572
                     /locus_tag="O0V33_002039"
     CDS             5742..6572
                     /locus_tag="O0V33_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCX8976661.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDEHITISQMEEAFEKGFKKGLNIELKPLELTEVQLAEVEELTEKYRSDEWMFRK
                     "
     gene            complement(6636..6854)
                     /locus_tag="O0V33_002040"
     CDS             complement(6636..6854)
                     /locus_tag="O0V33_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976662.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLVSFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(6868..7449)
                     /locus_tag="O0V33_002041"
     CDS             complement(6868..7449)
                     /locus_tag="O0V33_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976663.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            7554..8615
                     /locus_tag="O0V33_002042"
     CDS             7554..8615
                     /locus_tag="O0V33_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCX8976664.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENVGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDADEIALIQKAADIVDETYEYILTVAKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYFNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            8641..9198
                     /gene="efp"
                     /locus_tag="O0V33_002043"
     CDS             8641..9198
                     /gene="efp"
                     /locus_tag="O0V33_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCX8976665.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
BASE COUNT         3344 a         1265 c         1715 g         3058 t
ORIGIN      
        1 ctagaattta aatgagtact attaaaaagg acgtgaaata atttattaat tttgatttat
       61 ataaagaaaa aggatatgtt acgccgttct tattgatgct aacagcaatt tatttttcca
      121 ttttcacttt ttatatcagt caatatagtt taaaattaaa gacattatat aatattgaaa
      181 ctgactataa ctataagatt gttttgttat tgaaagaagg gaaaacgcat gaatcatgat
      241 aaatcaccaa taattttaat tggtttcatg ggtaccggta aatctacgat tggtaaatac
      301 gttgcagatg agcaaaattt atcatttatt gatatagatt catatatcga agaaaagtat
      361 aagttaacaa taccagaaat atttagtaaa catggtgaac aatatttcag gaatttagag
      421 ttcacatgtt tgcaagaatg tattaacact gcagatataa ttgctactgg tggtggtatt
      481 attgaaagtg aagaggtatt taattttttg aaaaatcaaa aaaacattat ttggttagat
      541 tgtaatattg atattatata tagtcgaatc aatgatgacc cacatcgacc taatgcaaat
      601 aataagacaa tcaagcagtt aaatgacttg tattgctcgc ggattttaag atataatgaa
      661 atcgcattca agaaatttga tagtcatttg ctatcaattt cagaaatata ttatgaattg
      721 ctaaatttaa taaaagcgag tgatcagtat tagagagaat agagcgttaa gactctatcg
      781 ccgaaggtgc aagtaattta ttacgaaact ctcaggcaaa aggataatac tgtaacgcgt
      841 tcctgaattg gtgatttata aacagggtag cgattgctat cctgttttta taattttaag
      901 ggggtatttc aatgtcaagt gatttaaaac aaacaccttt atatcaaaat tatgttgata
      961 gaggtgcaaa aattgtggaa ttcggaggat gggcgatgcc tgttcaattt tcaagtatta
     1021 aagaggagca taatgctgtt cgatacgaaa ttggcctgtt tgatgttagt catatgggtg
     1081 aaattgaagt gacaggtaaa gatgctagtc agtttgtgca atatttatta tcaaatgata
     1141 ctgataattt aactacttca aaagcattat atactgcttt atgtaatgaa gaaggcggta
     1201 ttattgatga tttagtaata tataaattag ctgacgacaa ttatttatta gttgttaatg
     1261 ctgctaatac tgaaaaagat tttaatcgga ttttaaaaca caaagagaaa tttgatgttg
     1321 aagtacaaaa tgtatcaaac caatatggtc aattagcaat acaaggacca aaagctagag
     1381 atttaattaa tcaattagtt gatgaagatg taactgaaat gaaaatgttt gaatttaaac
     1441 agggtgtcaa attatttgga gcgatcgtca ttttatctca gtcaggttac acaggtgaag
     1501 atgggtttga aatttattgt aatatcgatg atactgaaaa aatttgggat ggtttattag
     1561 agtataatgt tatgccatgt gggttaggcg ctcgtgatac attaagatta gaggctggat
     1621 tgccattaca tggacaagat ttaactgaat caattacacc atatgaaggt ggtatcgctt
     1681 ttgcaagtaa accattaatt gatgctgatt ttattggtaa atctgtatta aaagatcaaa
     1741 aggaaaatgg tgcaccaaga agaacagtgg gattagaatt acttgaaaaa ggaattgcaa
     1801 gaactggtta tgaagttatg gatttagatg gaaatattat tggagaagta acttcaggaa
     1861 cacagtctcc atcatcagga aaatcaattg cacttgcaat gataaaaaga gatgagtttg
     1921 aaatgggtag agagttgctt gttcaagttc gtaagcgtca attaaaagcg aaaattgtta
     1981 agaaaaatca aattgataaa taattaaaaa ggggtgtgca ttgtgagtca tcgttatata
     2041 cctttaactg aaaaagacaa gcaagaaatg ttacaaacaa ttggtgcaaa atctatagga
     2101 gaattattcg gtgatgtacc aagtgacatt ttattaaata gagatttaaa tattgctgaa
     2161 agcgaagcgg aaacaacgtt acttagaaga ttaaatcgca ttgcaagcaa gaatatcact
     2221 aaagaaacgc atacatcatt tttgggtgct ggtgtttatg atcattacgc gccatcagtt
     2281 gtggatgcta tgatttcaag atcagaattt tacacagcat atacgccata tcaaccggaa
     2341 atttctcaag gtgaattaca agccattttt gagttccaaa ctttaatttg tgaattaaca
     2401 gatatggatg tagcaaattc ttctatgtat gatgggatga ctagctttgc tgaagcatgt
     2461 attttagcat ttagtcaaac aaagaaaaat aaaattgtag tctcaaaagg tttgcattat
     2521 caagcattac aagtacttca tacttatgct aagacgcgta aggaattcga agtagtggaa
     2581 atcgatttag atggcactgt tacagatttg aaaaaattag aagcagcagt ggatgatgat
     2641 acagcagctg ttgcagttca atatccaaac ttctatggtt caattgaaga tcttgaaaag
     2701 attcatagct ttattgaaga taaaaaagca ttatttatcg tatatgcaaa tccattagca
     2761 ttgggattac ttacacctcc aggttcattt ggtgcagata ttgttgtagg tgatactcag
     2821 ccatttggaa ttcctgctca attcggtggg ccacattgtg gatattttgc aacgacgaaa
     2881 aagttaatgc gtaaagtacc aggtagatta gtcggtcaaa cacaagatga tgaaggtaat
     2941 cgtggctttg tattaacatt acaagcacgt gaacaacata ttcgacgcga taaagcgaca
     3001 tctaatattt gttctaacca agcattaaat gccctagcgt catcaattgc aatgtcagca
     3061 cttggtaaac aaggtatcta tgatattgca gttcaaaata tagaacatgc taactatgct
     3121 aaacaacaat ttattaaaaa aggttttgaa gtattagatg gtacatcttt caatgagttt
     3181 gtagtcaaat ttgacaaacc aattcaacaa gttaacgagg aattagtgaa gtacaatatt
     3241 atcggtggtt ttgatttagg tgttgtttca gacgacttta aaaatcacat gttaatagct
     3301 gtaactgaat taagaacaaa agatgaaatc gatacatttg tagaaaaggc tggtgagtta
     3361 aatgactagt aaatcaagtc cattaatttt tgaaagatct cgtgaaggca gatatgcata
     3421 ttcattacca ataagtgata ttaaaacaaa ttctgttgag tcattgttag atgataaatt
     3481 tattcgtaaa aacaaagcgg agtttcctga agttgctgaa ctagacttag tacgtcatta
     3541 tactgaactt tcaaataaaa atttcggtgt agataacgga ttttatccat taggttcatg
     3601 tacgatgaag tataacccta aaattaatga aaaggttgct agaataccag gattcagtga
     3661 atcacatcca ttacaagatg aagaccaagt tcaaggttca ttagaaatta tttatagttt
     3721 acaagaagaa ttaaaagaaa ttactggtat ggatgaggta acattacaac cagctgctgg
     3781 tgcacatggt gaatggactg cattgatgat atttaaagct tatcatgaga ataatggtga
     3841 aggtcatcgt gatgaagtca ttgtgccaga ttctgcgcat ggtacgaatc cagcctcagc
     3901 ttcatttgca ggatttaaat cagttactgt aaaatcaaac gaacgtggcg aagttgatat
     3961 tgatgacttg aaacgtgttg taaatgaaaa tacagcagct attatgttaa ctaatccaaa
     4021 cactttaggt attttcgaaa aaaatattat ggaaatccgt gaaatcgtcc ataatgctgg
     4081 tggtctatta tattatgatg gtgcgaattt aaacgctatt atggacaaag ttcgcccagg
     4141 agatatggga tttgatgctg ttcatttaaa cttgcataaa acatttactg gtccacatgg
     4201 tggcggcggt cctggttcag gtccagtcgg tgtagtaaaa gaactagcaa gttacttacc
     4261 aaagccaatg gttattaaag atggcgacaa atttaaatat gataatgaca ttaaaaattc
     4321 tatcggacgt gtaaaaccat tttatggtaa ctttggtatt tacttaagag cttatacgta
     4381 tattcgaact atgggagcaa ctggccttaa agaggtttcc gaagcagcgg ttcttaatgc
     4441 gaattatatt aaagcacgtt tatctgaaca ctttgaaata ccttataaac aatattgtaa
     4501 acacgagttt gtgttaagtg gtgttcgtca aaaagaattt ggtgtacgta ctttagacat
     4561 ggctaagcga ttattagatt tcggtgtaca tccaccaaca atctacttcc cattaaatgt
     4621 tgaagaaggt atgatgattg aaccgactga gacagagtct aaagaaacac ttgattattt
     4681 tatcgataca ttaattagta ttgctgaaga agctaaaaat gatcctgata aagtgttaga
     4741 agcaccatat acaactgtga ttgatcgatt agacgaagct acagctgctc gtaaaccaat
     4801 attaaagttt gaaaatctta aacaggaaaa ataaggtgtt aaacacatat tccgagaatt
     4861 attattctaa ctttgtatga agatttaagg ataatggttt taaaatcaat taaaaaagac
     4921 aatttctatt tgaaaaagaa aactaaaccg aagtaataac tcttagggtt tggtattatt
     4981 ctttcataga aattgtcttt tcacttttta gattgaggta attgaatcgt attgaaaatg
     5041 agttgaactc tctttattat gcagaaacta aatttaatga tctgtgaagc tagagaaata
     5101 tagtaacagt ctttgttaaa aaattaaaat ctgagtattg ttattgatga tcaattaaca
     5161 ctcagatttt attaaatcgt atattaaatt tacaaaacta ttttttagac tttatttttc
     5221 cagtccattt tttatagccg ccttttaaca tatagatatc tgtatatcca ttctttttca
     5281 aaatacgagc ggctctatag ctagcaatcc cattggcatc acataagtat accggttggt
     5341 cttttcttaa tccttggaat cgttgcctga acattgtcat aggaatattg cgagacccat
     5401 taatgtgacc gtagtcatag tcaactttct ctctaacatc gatgacttga gcttttctaa
     5461 tcccattatg gaattcattt tgatctaatt ctttaacagc tcgcttgtta agaatttgtt
     5521 gaacaatcat ataagcaata attgctataa ctaaaattat tgcgatgtac aaactagcac
     5581 tcatcttgca tcctccctaa ttaaacgata atataattat aagactgtta ggtcaaatta
     5641 tcaaaatatt tcactaaaga attgcaatta ttattcattt agttaaattg attaattagc
     5701 gaatcaaata ttatacattt tgctgatagg agttcttgaa tatgactgaa acttggaatt
     5761 ttattaatac aggaagtaaa gacccatatt ataatatggc aatggatgaa gcgttattaa
     5821 attttgtctc tagaggtgaa attgatccag ttatacgttt ttacacatgg aatcctgcaa
     5881 cattatcaat aggttatttc caaaggctac aaaaagaaat tgatattgat aaggttaaag
     5941 agaaaggttt tggtctcgta aggcgtcaaa ctggtggccg tggggtgctt catgataaag
     6001 agctaactta cagtgtgata gtgcctgaat cgcatcccaa tatgccttct actgttacag
     6061 aagcgtacag agtgatttca caaggattat tagaaggatt taaaaactta ggatttgata
     6121 cttattttgc ggtacctaaa acgcctgaag agcgtcaaaa attaaaacaa cctcgaagtt
     6181 cagtgtgttt tgatgcacca agttggtatg agctagttgt tgaaggccgt aagattgctg
     6241 ggagtgctca aacgagacaa aagggcgtaa tcctccaaca cggttcgatt ttacaagata
     6301 ttgatattga cgaattgttt gatatgttta tttataaaaa tgaaagatta aaattaaaaa
     6361 tgaaagaggc attcgttgaa aaagcagttg ccatcaatga tatttcagat gaacacatta
     6421 ctattagtca aatggaagaa gctttcgaga aaggttttaa gaaaggttta aatatagaat
     6481 taaagccact tgaattaaca gaagttcaat tggcagaagt ggaagaatta actgaaaaat
     6541 atcgttcaga tgaatggatg tttagaaagt agtttttaga ataaaaaatg gaagcacgta
     6601 gactatttta actacaagtg cttccatttt tttatctatt ttcttctttg atttctttta
     6661 tacttacgca ttgctttgcg atatttgcgt tggtcttcag ttaagatgaa gaaataataa
     6721 atcagataaa taataccagc tattacagca aagcttacta acattctgac gattgaaaaa
     6781 ataaaggcat ctagattcat tactaatcca atcgcagcaa ttataagtac aaggtaaaat
     6841 attatattac gcattaaaag actcctatca tgattttgca tttaagttta attttgacaa
     6901 cgcttgttgt ccttttgtaa gttgtttttt atctttattt tgatagccat ttttaatatc
     6961 atcaaatgca tgattcaaat cagagtttaa tttatcgata tttttagacg tttttttatc
     7021 atcatcacta agtgataaac catcgatagc atctttgtat gaagtgtgag cactttgaat
     7081 atcattagaa atttgattta atttcgattg aacattctta tcgcctttgt ttttacttac
     7141 atccttttca atatcgttat aggatttcaa agctttagca actttgtcgt agtatgttga
     7201 tgtaacattc atcaatttaa ttttcgtatt attagatgca gtttttttac tattttcttt
     7261 atccttttca agccctttaa ttttagattt agaatcacta atttgttgat taagttcttg
     7321 aatatctagt ttgagttgat gattatcttc tcttaaatct gttgtttttt cttctaatgg
     7381 tgctaaattt tgtgatccac atccagctaa caataatgtt gcgccaacaa ttgaaaccaa
     7441 ttttttcatt actatctccc taaaatcaat atctattatt ttttatcctt attatgatat
     7501 tattttaatg tataaagttg aaatgcacaa acgtttcact aggaggtaat aagatgagca
     7561 gaataacaca agtgcatcgt attttagaac aaaaacattt agatgcgatt atcattcttt
     7621 cggattacaa tagaagatat ttatcaggtt ttactggtac cagtggagca cttatcatat
     7681 ctaaagataa acaatatttg ataacagact ttagatatat tgatcaagca actaagcagg
     7741 cgccaaatta tgaaattatt aatcgtaaat ctactatcat tggtgagatt aaagaattgc
     7801 tacaccaaga gaattttgaa aatgtaggct ttgaggggca ccatgtgagt tatgatacat
     7861 accttgaatt aaataaaagc cgtatatcat taataagcat ttctaatact gtagataaaa
     7921 ttagagacgt aaaagatgct gacgaaattg cattgattca aaaagcagca gatatcgttg
     7981 atgaaacata tgaatacata ctaacggttg caaaagctgg tatgactgaa aaagaattaa
     8041 aggcaatatt agaaagtaaa atgctagaat taggagcaga tggaccatca ttcgatacga
     8101 ttgtagcatc tggtcataga ggtgcattac cacatggtgt tgcaagtgac aaaattattg
     8161 aaaaaggcga catgataaca ttggattttg gtgcttattt caatggatat tgttcagata
     8221 ttactagaac atttgctatt ggagaaccag atcctaaact gaaagaaata tatcaaatag
     8281 tacttgaatc tcaaatgaaa gcaattaatg agattagacc tggcatgact ggtgcagaag
     8341 ctgatgccat ttcaagaaac tatttagagt caaaagggta tggaaaagaa tttggacatt
     8401 cactaggaca tggtattggt ttagaaatcc atgaagggcc aatgctggct cgtacgatac
     8461 aagataaact tcaagttaac aactgtgtta cagtagaacc tggtgtttat atagaaggtt
     8521 tgggcggtat aagaatagaa gatgatattt taattacaga aaatggttgt caagtcttta
     8581 ctaaatgcac aaaagacctt atagttttaa cataagcgtg taaaatgagg aggaaactga
     8641 atgatttcgg ttaatgattt taaaacaggt ttaacaattt ctgttgataa cgctatttgg
     8701 aaagttatag acttccaaca tgtaaagcct ggtaaaggtt cagcattcgt tcgttcaaaa
     8761 ttacgtaatt taagaactgg tgcaattcaa gagaaaacgt ttagagctgg tgaaaaagtt
     8821 gaaccagcaa tgattgaaaa tcgtcgcatg caatatttat atgctgacgg agataatcat
     8881 gtatttatgg ataatgaaag ctttgaacaa acagaacttt caagtgatta cttaaaagaa
     8941 gaattgaatt acttaaaaga aggtatggaa gtacaaattc aaacatacga aggtgaaact
     9001 atcggtgttg aattacctaa aactgttgaa ttaacagtaa ctgaaacaga acctggtatt
     9061 aaaggtgata ctgcaaccgg tgctactaaa tcggcaactg ttgaaacagg ttatacatta
     9121 aatgtacctt tatttgtaaa cgaaggtgac gttttaatta tcaacactgg tgatggaagc
     9181 tacatttcaa gaggataatc tctaatttgt taacaaatag cttgtattca ctatactgat
     9241 ttaacgtaag atattccaaa taagtctcat aaagctattg cctaaaatga ttataggtta
     9301 tatgctgata tgaggctttt tatttttaaa ataaatttta aaatataaaa cgaatcgtct
     9361 aattggtagt aaaaaaacat ag
//
LOCUS       DAKSQE010000095         9251 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.095, whole genome shotgun sequence.
ACCESSION   DAKSQE010000095 DAKSQE010000000
VERSION     DAKSQE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9251
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.095"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(112..252)
                     /locus_tag="O0V33_002044"
     CDS             complement(112..252)
                     /locus_tag="O0V33_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HCX8976666.1"
                     /translation="MKKLLNKVIELLVDFFNSIGYRAAYINCDFLLDEAEVPKELTQL
                     HE"
     gene            complement(256..819)
                     /locus_tag="O0V33_002045"
     CDS             complement(256..819)
                     /locus_tag="O0V33_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HCX8976667.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLI
                     VMYTLAYILNIFIFTLITNISFYLIRRYAHGAHAPSSFWCYIESITLFIVLPLLVLHF
                     HINETLMMFLALLSVGVVIKYAPAATKKKPIPARLVKQKRYFSIIISTILFIITLFVK
                     EPYTQFIQLGIIIQAITLLPIYYSKED"
     gene            1055..1189
                     /locus_tag="O0V33_002046"
     CDS             1055..1189
                     /locus_tag="O0V33_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="HCX8976668.1"
                     /translation="MSCLILRIFILIKEGVISMAQDIISTIGDLVKWIIDTVNKFTKK
                     "
     gene            complement(1791..2576)
                     /locus_tag="O0V33_002047"
     CDS             complement(1791..2576)
                     /locus_tag="O0V33_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HCX8976669.1"
                     /translation="MKVQIYQLPIIFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKSG
                     KLINEYDKVHLVPMLREHEFLTAGENVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPVFKSIKLDLYK"
     gene            complement(2937..3563)
                     /locus_tag="O0V33_002048"
     CDS             complement(2937..3563)
                     /locus_tag="O0V33_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCX8976670.1"
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYENQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYN
                     PLVDEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
     gene            3760..4974
                     /locus_tag="O0V33_002049"
     CDS             3760..4974
                     /locus_tag="O0V33_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976671.1"
                     /translation="MSYHWFKKMLLSTSMLILSSSSSLGLATHTVEAKENLNGEKPTT
                     NLNHNVTSPSVNSEMNNNETGTPHESNQAGNEGTGSNSRDANPDSNNVKPDSNNQNPS
                     PDSKPDPNNPNPGPNPKPDPDKPKPNPEPKPDPDKPKPNPDPKPDPDKPKPNPDPKPD
                     PNPNPNPKPDPNKPNPNPSPNPNQPGDSNQSGGSKNGGTWNPNASDGSNQGQWQPNGN
                     QGNSQNPTGNDFVSQRFLALANGAYKYNPYILNQINQLGKEYGEVTDEDIYNIIRKQN
                     FSGNAYLNGLQQQSNYFRFQYFNPLKSERYYRNLDEQVLALITGEIGSMPDLKKPEDK
                     PDSKQRSFEPHEKDDFTVVKKQEDNKKSASTAYSKSWLAIVCSMMVVFSIMLFLFVKR
                     NKKKNKNESQRR"
     gene            complement(4999..5742)
                     /locus_tag="O0V33_002050"
     CDS             complement(4999..5742)
                     /locus_tag="O0V33_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCX8976672.1"
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLPFVQYSGIELTKAVIY
                     IQLVLFLIAATTIILINLKIKNPTKLELEVKEPKKYIIPWALLGFALVMIYQMVVSIV
                     YTQIFGTQQTSPNTERLIVIARKIPLFIFFVSIVGPLLEEYVFRKVIFGELFNAIKGN
                     RIVAFAIATTVSSLIFALAHNDYKFIPIYFGMGVIFSLAYVWTKRLAVPIIIHMLQNG
                     FVVIFQLLNPEALKKATEQANFIYHIFIP"
     gene            5917..6201
                     /gene="groES"
                     /locus_tag="O0V33_002051"
     CDS             5917..6201
                     /gene="groES"
                     /locus_tag="O0V33_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCX8976673.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            6277..7893
                     /gene="groL"
                     /locus_tag="O0V33_002052"
     CDS             6277..7893
                     /gene="groL"
                     /locus_tag="O0V33_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCX8976674.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDATIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEVGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(7962..9134)
                     /locus_tag="O0V33_002053"
     CDS             complement(7962..9134)
                     /locus_tag="O0V33_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002476300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCX8976675.1"
                     /translation="MASYEKRGNTWRYRISLGKDAETGKYKYISNSGFKRKSDAKHHA
                     EMVERQLRNGDYIAPSTSTFKQVADDWISQYANEVKVSSVRAREKAINHAIERFNNKP
                     IQTINKHEYQRFVNDISAQYSKNYVDSIIASTNMIFKYAYDMKLIRIMPSEGIKRPKK
                     KISVEELEDTEIHKKFLEKDELFQFLEVAKNHHSPQNSFEVFCTLAYTGMRAGELLAL
                     KWSDIDFENNTINITKTYYNPNNNKKQFQILTPKTESSIGKISVDPHVIKLLKNYKTD
                     VQDTWKNELYVDNNFVFTDVNGYPLVIKKLQLWIKAILKKTDITNKQISTHSFRHTHC
                     ALLIEAGVHIKEIQERLRHKDINTTMNIYAKITNSYKKDASQKFSKLMENVSKDLF"
     gene            complement(9148..>9251)
                     /locus_tag="O0V33_002054"
     CDS             complement(9148..>9251)
                     /locus_tag="O0V33_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCX8976676.1"
                     /translation="EELFPNKGEKSTTLHKEILQTDSRLSSDIFKEY"
BASE COUNT         3221 a         1446 c         1556 g         3028 t
ORIGIN      
        1 tccactaata aatgcagata taaagaacat taatgaaact tgaacaatta caaataaaac
       61 gtaattataa tcatttaatg cttccactta ctatcacact ttctcttata gttattcgtg
      121 taattgagtt aattcttttg gtacttcagc ttcatccaat aaaaaatcac aatttatata
      181 ggctgctctg taaccgatgc tgttgaaaaa gtcaactaaa agctcaataa ctttgttgag
      241 taattttttc ataatttagt cctcctttga atagtatatt ggtagtaatg taatagcttg
      301 tataataata cccagttgaa taaattgggt atatggttcc tttacaaaaa gtgtgataat
      361 gaaaagaatt gtactaatta taattgaaaa atatcttttt tgctttacaa gccttgccgg
      421 gataggcttc ttcttagtag ctgcaggtgc atatttaatc actacgccaa cacttaataa
      481 tgctaaaaac atcattagtg tttcattaat atgaaaatgt agtactaata gaggtagaac
      541 aataaaaagt gtaatgcttt ctatataaca ccaaaaagat gaaggtgcat gtgcaccatg
      601 tgcatacctt ctaattagat aaaacgatat attagtaatt aaagtgaaga taaaaatgtt
      661 taaaatatag gcaagagtat acataacaat cagttttcct atatttttag ctaagacctg
      721 catccctaat cgtacttgca aaaattgaat atgatctaag ttatttctct tttgaagata
      781 cgtggcaaac tggtcaattt tgttatcaaa ataattcaat tttacaccac tctcctcact
      841 gttattatac gatttagtac aatcttttat cattatattg cctaactgta ggaaataaat
      901 acttaactgt taaatgtaat ttgtatttaa tattttaaca taaaaaaatt taccgttaag
      961 aataaaaaac gactagttaa gaaaaaatgg aaaataaatg cttatagcat gttttaatat
     1021 aactagatca cagagatgtg atggaaaata gttgatgagt tgtttaattt taagaatttt
     1081 tatcttaatt aaggaaggag tgatttcaat ggcacaagat atcatttcaa caatcggtga
     1141 cttagtaaaa tggattatcg acacagtgaa caaattcact aaaaaataag atgaataatt
     1201 aattactttc attgtaaatt tgttatcttc gtatagtact aaaagtatga gttattaagc
     1261 catcccaact taataaccat gtaaaattag caagtgagta acatttgcta gtagagttag
     1321 tttccttgga ctcagtgcta tgtatttttc ttaattatca ttacagataa ttatttctag
     1381 catgtaagct atcgtaaaca acatcgattt atcattattt gataaataaa atttttttca
     1441 taattaataa catccccaaa aatagattga aaaaataact gtaaaacatt cccttaataa
     1501 taagtatggt cgtgagcccc tcccaagctc gcggcctttt tttattgtaa taaagtaggg
     1561 aagagttaat cctcattatg agatccaccg ttaaaatata tgaataagtc taatgttgga
     1621 aaaggtcaaa aaaataatca atttaattaa gaaaatcatt catttgctaa gggcgaaact
     1681 ggttcttact gagttatcta ttataaaaaa ataaacatag gcttatgaaa aatcgtctca
     1741 taaatctatg tttagtcatg acatgtgtta aatattattt cgtgtgcttt ttatttatac
     1801 aaatctaatt taatactttt aaatacaggt atattttcgc gttgctgttc tacttcattt
     1861 aagtttaaat ctacagtcaa aatatctgcg gattcattta attctccaac taaatctccg
     1921 tttggattta taactatcga atgaccagca tattctgtgt taccatcgaa tccagtgcta
     1981 ttagttccaa tgacaaacat attattttca attgcacgtg cctttagtaa agaatgccaa
     2041 tgttgaagac gtgacatagg ccattgtgcc acataaaatg caattttagc accactacga
     2101 gcaggatatc ttaataattc tggaaaacgt aaatcataac agataagttg ggtcacataa
     2161 gtaccgtcag acaattgaaa gggttcagct acgttttcgc cagcggttaa aaattcatgc
     2221 tctcttaaca taggaactaa atgaactttg tcgtattcat taatcagttt gccactttta
     2281 ttcacactaa aagctgtatt aaatatttga ttgtttctaa tgttagaaac tgacccagct
     2341 acgatatcga ctttatattt ttcagctaaa tgtttaataa atgaaaaact ttgtcctaga
     2401 ttattatctg ctttttcatt taaatgctct aaatcatagc cattattcca catttcaggt
     2461 aaaacgacta catctacttc agcattcata tttttttcga accattgcgt tatttgagtt
     2521 tcatttttag aactatctcc aaaaataatc ggtaattgat aaatttggac tttcataaca
     2581 tcacatcctt gatagatctt atatataaca tactaaaagt tatgttgaaa cgcaaaaaac
     2641 aagcccagga cataaaatca aagtcctagg ctctacaatg ttatattgac agtagttgat
     2701 ggggccccaa catagagaaa ttggaacacc aatttctaca gacaatgcaa gttggggtgg
     2761 gctctaacgt aaagaaatac tttttcttta gaaattagta tttcttatac atgagtttta
     2821 ctcatgtatt cctattctta agtacacatt agcagcggct aatgtgtaag aactactaca
     2881 taatgaataa ctaatgattc tatatcattt ctgtcccatt cccaacaata tattgattat
     2941 ttttttatta cgaaacgatc ttccactgga ttaaatgttt tttcgccagc agcttcacga
     3001 atatcaccaa atggcatttg agcaataagt ttccaacttt taggaatatt aaattcattt
     3061 gaagtcatct catcaacaag tggattatag tgttgtaatg aagcacctat gcctttagta
     3121 gctaatgcag tccaaattgc aaattgatgc atggcatttg tttgagttga ccatattgca
     3181 aaattatcat agtagtttgg catttgttct tgtaaaccac ttacaacatc ttgattttca
     3241 taaaacaaaa ttgtaccgta tgaatgtttg aagttatcaa ttttttgttc agttggctcg
     3301 aaatcacgat tctctcccat gacttctttt aaaattgctt ttgtgttatc ccaaaattta
     3361 ttattgttgt catttaataa gagaacaatt ctagttgatt gagaattaaa tgatgaagga
     3421 acatgtttaa ctgcatgtgc aatcattgat tctaattcgt catcgctaat tgatattgaa
     3481 tctttcaaat tatatattga acgtctttct tccattgcgt tgtcaaaagt cattgctttt
     3541 ttatcttttt taaataaacc cataatcatt actccttctt tagtaaagaa tacttaatag
     3601 acttagtata aaatttatac tcgaacttgt aaagtaatat ttacgaaaat ttcaagaata
     3661 ttaatattca ttttcaaatt ccaaatataa atgcattttc aacgcatatt tattatactt
     3721 agattaatac ttatatgaaa aagggaggtg tctcgtgaaa tgtcatatca ttggtttaag
     3781 aaaatgttac tttcaacaag tatgttaatt ttaagtagta gtagtagttt agggcttgca
     3841 acgcacacag ttgaagcaaa ggaaaactta aatggagaaa agccaacgac taatttgaat
     3901 cataatgtaa cttcaccatc agtaaatagt gaaatgaata ataatgagac tgggacacct
     3961 cacgaatcaa atcaagctgg taatgaagga actggttcga atagtcgtga tgctaatcct
     4021 gattcgaata atgtgaagcc agactcaaac aaccaaaacc caagtccaga ttcaaaacct
     4081 gacccaaata acccaaaccc aggtccgaat ccgaagccag acccagataa gccgaaacca
     4141 aatccggaac caaagccaga cccagataag ccgaaaccaa atccggatcc aaaaccagat
     4201 ccagacaaac cgaagccaaa tccggatcca aaaccagatc caaatccgaa tccgaatcca
     4261 aaaccagacc ctaataagcc aaatccaaat ccgtctccaa atcccaatca acctggggat
     4321 tccaatcaat ctggtggctc gaaaaatggg gggacatgga acccaaatgc ttcagatgga
     4381 tctaatcaag gtcaatggca accaaatgga aatcaaggaa actcacaaaa tcctactggt
     4441 aatgattttg tatcccaacg atttttagcc ttggcgaatg gggcttacaa gtataatccg
     4501 tatattttaa atcaaattaa tcaattgggg aaagaatatg gtgaggtaac tgatgaagat
     4561 atctacaata tcatccgtaa acaaaacttc agcggaaatg catatttaaa tggattacaa
     4621 cagcaatcga attactttag attccaatat ttcaatccat tgaaatcaga aaggtactat
     4681 cgtaatttag atgaacaagt actcgcatta attactggcg aaattggatc aatgccagat
     4741 ttgaaaaagc ccgaagataa gccggattca aaacaacgtt catttgagcc tcatgaaaaa
     4801 gatgatttta cagttgtaaa aaaacaagaa gataataaga aaagtgcgtc aactgcatat
     4861 agtaaaagtt ggctagcaat tgtatgttct atgatggtgg tattttcaat catgctattc
     4921 ttatttgtaa agcgaaataa aaagaaaaat aaaaacgaat cacagcgacg ataatccgtg
     4981 tgtgattcgt tttttatatt atggaataaa aatgtgatat ataaaattcg cttgttccgt
     5041 ggcttttttc aaagcttcag gattaagtaa ttggaatata acgacaaatc cattttgtaa
     5101 catatgaata ataattggaa cagcaagtcg tttcgtccat acatatgcta atgaaaaaat
     5161 gacgcccata ccaaagtata taggaataaa tttatagtca ttatgcgcta atgcaaagat
     5221 taatgagctt acagtcgttg caatagcaaa tgcgacgata cgattacctt taattgcatt
     5281 aaataattcg ccaaagatta cttttctaaa tacatattct tctaatagag gaccaacgat
     5341 tgatacgaaa aagataaaca atggaatctt acgtgcaata acgattaaac gctcagtatt
     5401 tggacttgtt tgttgtgtgc caaaaatttg agtatataca atactaacta ccatttgata
     5461 aatcattacc agtgcaaatc caagcaatgc ccatggaatg atatattttt taggttcttt
     5521 aacttctaat tctaattttg ttggattttt aatttttaaa ttaattaaaa taatcgtcgt
     5581 ggcggcgatt aaaaatagaa caagttgtat gtaaatgact gctttagtca gttctatgcc
     5641 actatattgt acaaatggta atttttttac aatgagaagc ggtaaaaatt gagacaatat
     5701 ataaataata acagttagca atgatgccca tagtcttgtc ataattttcc tccaaatatt
     5761 tgtttataat ttattttatc gtaaataact tgaagttaca aaacttaatt gaaaggttat
     5821 gacttgaagt tttgaccaaa tttgattatt ataaatgtat gttagcactc tataaagtta
     5881 agtgctaaac tttaggtttt ttaaggagga acaatcatgc taaaaccaat tggaaatcgt
     5941 gtgattattg agaaaaaaga acaagaacaa acaactaaaa gtggtattgt tttaactgat
     6001 agtgctaaag aaaaatcaaa cgaaggcgtt atcgttgcag taggaacagg acgcctatta
     6061 aatgatggta caagagtgac tcctgaagtg aaagaagggg accgtgtcgt gttccaacaa
     6121 tatgcaggta cagaagttaa acgagataat gagacttatt tagttttaaa tgaagaagat
     6181 attttagcgg taattgaata atatagaata taattcatag atagattgta aagaacgaaa
     6241 atgaaatatg actaaacaaa tggaggttta tcacttatgg ttaaacaatt gaaattctct
     6301 gaagatgcac gtcaagcaat gttacgtggt gttgaccaat tagcaaatgc agttaaagta
     6361 acgattggtc ctaaaggacg taatgttgta ttagataaag agtttacagc acctttaatt
     6421 acgaacgatg gtgtaacgat tgctaaagaa atcgaattag aagatccata tgaaaatatg
     6481 ggggctaaac tagttcaaga agtcgcaaat aagacaaatg aaattgctgg tgacggtacg
     6541 acaactgcaa cagtattagc tcaagcaatg attcaagaag gcttgaaaaa tgttacaagt
     6601 ggtgcaaacc cagttggttt acgacaaggt atcgacaaag cagttaaagt tgctgttgaa
     6661 gcgttacatg aaaattctca aaaagttgaa aataaaaatg aaattgcgca agtaggtgcg
     6721 atttcagcag cagatgaaga aattggacgc tatatttcag aagctatgga aaaagtaggt
     6781 aacgatggtg tcattacaat tgaagaatca aatggcttga acactgaact tgaagtggtt
     6841 gaaggtatgc aatttgatcg tggttatcaa tcaccgtata tggttactga ttcagataaa
     6901 atggttgctg aattagaacg cccatatatt ttagtaacag ataaaaaaat ctcttctttc
     6961 caagatatct tacctttatt agaacaagtg gttcaatcta atcgtccaat cttaattgta
     7021 gctgatgaag ttgaaggcga tgcattaaca aatatcgtgc taaaccgtat gcgtggcaca
     7081 tttacagctg ttgcagtaaa agcacctggt ttcggtgatc gtcgtaaagc aatgttagaa
     7141 gatttagcta ttttaactgg tgcgcaagtg attacagatg atttaggctt agaccttaaa
     7201 gatgcaacga ttgatatgtt aggtactgca agtaaagtag aagttactaa agataataca
     7261 actgttgtag atggtgacgg tgacgaaaac agcattgatg cacgtgtaag ccaattgaaa
     7321 tctcaaattg aagaaactga atctgacttt gatcgtgaaa aattacaaga acgcttagct
     7381 aaattagcag gcggtgttgc agttatcaag gtaggtgcag caagtgaaac agaacttaaa
     7441 gaacgtaaat tacgtatcga agatgcttta aactctacac gtgcagcagt tgaagaaggt
     7501 attgttgcag gtggtggtac tgcactagta aatgtttacc aaaaagtaag tgaaattgaa
     7561 gctgaaggcg acattgaaac aggtgtgaat attgtactta aagcattaac tgcaccagtt
     7621 cgtcaaattg ctgaaaatgc aggattagaa ggctctgtca tcgttgaacg cttgaaaaat
     7681 gcagaaccag gtgttggttt taatgctgct acaaacgagt gggttaatat gttagaagta
     7741 ggtatcgttg atccaactaa agtaacacgc tcagcattac aacatgcagc aagtgttgcg
     7801 gcaatgttct taacaactga agcggttgtg gcatcaattc cagaaaaaaa taatgaccaa
     7861 cctaacatgg gtggcatgcc aggaatgatg taaaatgact gttaaacgcc gattttataa
     7921 cgtttgtaat attgggtggt cataatttgg tcatagaaat tttaaaataa atcttttgag
     7981 acgttttcca tgagtttact aaacttttga gaagcgtctt ttttgtatga gttcgtaatc
     8041 ttagcgtaga tgttcatagt ggtatttata tctttatggc gcaagcgttc ttgtatttcc
     8101 ttaatatgca caccagcctc tataagtaac gcacaatgag tatgacgaaa tgaatgagtg
     8161 cttatttgtt tattagttat gtcagtcttt ttaagtatag cttttatcca taattgtagt
     8221 tttttaatta caagagggta gccgttaaca tcagtaaaaa cgaaattatt atctacatac
     8281 aattcgtttt tccatgtgtc ctgcacgtcg gttttataat ttttaagtaa tttaatcaca
     8341 tgaggatcca ctgaaatttt tccgattgag ctttcagttt ttggtgtaag tatttgaaat
     8401 tgctttttat tgttattcgg attgtaataa gtctttgtaa tattgattgt gttattctca
     8461 aagtctatat cagaccattt caatgccaat aattcacctg cacgcatgcc tgtatatgct
     8521 aatgtacaaa acacctcaaa gctgttttgg ggtgaatggt gatttttagc aacctccagg
     8581 aattgaaata attcatcttt ttcaagaaac tttttatgta tctcagtatc ttctaattct
     8641 tccacactaa ttttcttttt aggtcgttta ataccctcgc taggcattat tcttattaat
     8701 ttcatatcgt atgcgtactt aaatatcata tttgtagagg ctataatgct atcaacataa
     8761 ttcttgctat actgtgcgct tatatcgttt acaaaacgtt gatattcatg tttattgata
     8821 gtttgtattg gtttattgtt aaagcgttct atggcgtggt ttatggcttt ctcgcgtgct
     8881 ctgacactac ttacttttac ttcgttagca tattgtgata tccaatcgtc agcaacctgt
     8941 ttaaatgtag atgtggacgg tgcgatataa tcgccatttc ttaactgacg ctcaaccatt
     9001 tcagcgtgat gtttagcgtc tgatttgcgt ttaaaacctg agtttgaaat atatttatat
     9061 ttgcccgttt ctgcgtcttt tcctagtgat atacgatagc gccatgtatt tccgcgtttt
     9121 tcataacttg ccatttgatc acctcgatta atattcttta aaaatatcac tagataaacg
     9181 gctatcagtt tgtaaaattt ctttatgtag agtcgtactt ttttcgcctt tatttggaaa
     9241 aagctcttca a
//
LOCUS       DAKSQE010000096         9187 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.096, whole genome shotgun sequence.
ACCESSION   DAKSQE010000096 DAKSQE010000000
VERSION     DAKSQE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9187
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.096"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..197
                     /gene="sarR"
                     /locus_tag="O0V33_002055"
     CDS             <1..197
                     /gene="sarR"
                     /locus_tag="O0V33_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HCX8976677.1"
                     /translation="KEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVT
                     DTQKANIQKLISELEEYIKN"
     gene            complement(386..1222)
                     /locus_tag="O0V33_002056"
     CDS             complement(386..1222)
                     /locus_tag="O0V33_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HCX8976678.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGTIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(1222..1836)
                     /gene="ureG"
                     /locus_tag="O0V33_002057"
     CDS             complement(1222..1836)
                     /gene="ureG"
                     /locus_tag="O0V33_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HCX8976679.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFVINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            complement(1849..2538)
                     /locus_tag="O0V33_002058"
     CDS             complement(1849..2538)
                     /locus_tag="O0V33_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HCX8976680.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNNPWIAWYHQQMQDKKAKLNPAICFTMLGHYLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEETRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            complement(2531..2983)
                     /gene="ureE"
                     /locus_tag="O0V33_002059"
     CDS             complement(2531..2983)
                     /gene="ureE"
                     /locus_tag="O0V33_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HCX8976681.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIVVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            complement(2996..4711)
                     /gene="ureC"
                     /locus_tag="O0V33_002060"
     CDS             complement(2996..4711)
                     /gene="ureC"
                     /locus_tag="O0V33_002060"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HCX8976682.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFPNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDYEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            complement(4708..5118)
                     /locus_tag="O0V33_002061"
     CDS             complement(4708..5118)
                     /locus_tag="O0V33_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HCX8976683.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDEYAGVFGDNGTENVNKKGGKRS"
     gene            complement(5132..5434)
                     /locus_tag="O0V33_002062"
     CDS             complement(5132..5434)
                     /locus_tag="O0V33_002062"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HCX8976684.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            5694..6599
                     /gene="yut"
                     /locus_tag="O0V33_002063"
     CDS             5694..6599
                     /gene="yut"
                     /locus_tag="O0V33_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HCX8976685.1"
                     /translation="MKMIDVLLKNISQVVLISNKWTGLFILIGLFVADWTVGLAAMIG
                     SIIAYAFARYINYSEAEINDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHVIQSLFEGFSQVFIEASVIGGVCILIGILIASRKAALLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            6814..7968
                     /locus_tag="O0V33_002064"
     CDS             6814..7968
                     /locus_tag="O0V33_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCX8976686.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIGDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKEYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELKSVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKASNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYTAESQIRNATSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF"
     gene            8181..9089
                     /locus_tag="O0V33_002065"
     CDS             8181..9089
                     /locus_tag="O0V33_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCX8976687.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDIEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWE
                     ETTAKDGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
BASE COUNT         3071 a         1477 c         1597 g         3042 t
ORIGIN      
        1 ctaaagagat tgctaagtgc tcagagttca aaccttacta tttaactaaa gctttacaaa
       61 agctaaaaga tttaaaattg ttatcaaaga aaagaagttt acaagacgaa agaacagtta
      121 ttgtttatgt tacagataca caaaaagcaa atattcaaaa actgatttca gaattagaag
      181 aatacattaa aaattaaatc aaggttaatt gcgtttaata acattgaacg ataacaattt
      241 attaaaacga agttatttat tcagcattgg gacataaaat taacttaaaa tttaaatatt
      301 gaagatgctt taattaaagt taaagactag ccatacctta tttcagctta ttaagcttga
      361 aacaaggtac actagtcttt ttattttaat attttcttag aaaatcaagt ttacgatcat
      421 aaatattttc tgcgatatag ctttggatgg ttccaagtat tttctctata atttgtgtgc
      481 gataagcaaa aattctaact gcaaaaccat gtgtaggcaa ttgagaaata gcaacacgac
      541 aatcgaatgt attgctgtaa gaactaatgg tttcataaac tgaatcgatt agtttttggt
      601 taacatcttc gtgtatgaag taagcagagc cataatgaga gtaatgttcc atataaccta
      661 tttcattgat cgattgttta ttaggattca ataataaatt atcatatgtg actaactcat
      721 cttcaatata aatttcattt attaaatgca tatattgata cttgaaggct tcgccagttt
      781 ttgaataacc aggagttaaa atatcagtat aaaataatga actagaatta ttgagattga
      841 acgtattgtg ttgataaaat ttagcatttt catatgcgat gattggatca gcgacatatt
      901 ctaaatatgc gttatctttc aaattaaaag tttgatactg ctcaacatga ttggaaggtg
      961 tcttgtatat ttttgttgca ccttgagatg tcaatgtcac tttagcgtta tcttcgacat
     1021 tcacattcat acggtaacga tctccatcta aatagccacc acctacattt actatataaa
     1081 atgtaggaat ggtagattga tttagataaa ctggacgtat cactttaagt gctttttcaa
     1141 agaaaatatc tcttgacact gatcgattgc catcaaaaaa cactgttaaa tcaagttgcc
     1201 cagtccattg ttgttcatcc attatgataa tcctttgagt aaagtgtcgc gttcaatcca
     1261 atcgataact tcatccaaac cttcgtcggt ttttaagtta gtaaaagtaa atggacgttt
     1321 accacgaaat actttagtat cttcagccat ttgttctaat gatgcaccaa catagggagc
     1381 taaatcagtt ttgttaatta caaagaaatc tgacttaatc ataccttgac cacctttacg
     1441 tggaatcttt tcaccttgag caacatcgat aatataaatt gaaaagtcaa caagttctgg
     1501 actaaatgtt gctgctaagt tatcgccacc agattctatg aaaattagtt ctatatcgtc
     1561 atgacgttct aataattcgt ctattgctgc aaagttcata gatgcatctt cacgaatcgc
     1621 agtatgagga catccaccag tttcaacacc aatgattcga ctttcaggta gaactcctga
     1681 atttactaat atcttttcgt cttcttttgt atatatatca tttgtaataa cgccgatgct
     1741 catttctttt gaaagacgtt ttacaacttt ttcaattaat tgtgttttac ctgcacctac
     1801 aggaccacca ataccaattt taatcggatt tgccacaatt ataacctcct atgatatgaa
     1861 aattctaaca ttgacattct catgcgccat ttgatttagt tctaaaccag gcgctgtcat
     1921 gccaaaatct gcttctttta attcgaaaat ctgctttctt gtttcttcta tataaggaat
     1981 catgtgagta actatctttt gaccagcagt ttgtccaagt ggaatagcac gaacagcatt
     2041 ttgagttaaa cttgaaacat tttgatataa atagtaatca ataatcgttt caatatctac
     2101 acctaaataa tggcctagca tagtaaaaca aatagctgga tttaactttg ctttcttatc
     2161 ttgcatttgt tgatgatacc aagcaatcca tgggttatta taaagttcta aagccaattt
     2221 aaccatgcga gtccccattt gttttgcacc aacacgtgtt tctttaggta agttttgaac
     2281 aaacatcagc ttatctatgt gtaatacttt ttgtgtatca tcattttcca atgcatcata
     2341 aactaaacgc atggctaaac catcagaata ggtaagttgc tcttgtaaaa acatttttaa
     2401 ccaagcaata aaagtatgat catcatgaat tatatttcgt tgaatatatg tttcaagacc
     2461 aaatgaatga ctgaaagcac ctgttggaaa ttgtgaatca cagaactgaa ataatcttaa
     2521 gtgtgtatga tcaatcatga gaatgtccta tatgtctaaa agccttatta actttacggt
     2581 cttctcgaac atatgggatg cctaaacttt ttaataaatc ttcaactaaa taatcatatt
     2641 gtactagcat ttcagtctct gtaaattgtg ctggcaaatg acgatttcct aattgatggg
     2701 ctatatctcc catttcttgc aatgttcttg gttgaatcac taaaagatct tctgaattaa
     2761 catccacaac aatcatatta tggtcatctg cgtataaaat atctccatat tgtaagtcaa
     2821 taggttgttt taaacgaatg ccaatttcag tgccatgatc tgttacgact ctttgaatac
     2881 gtttaacgag atctgaattt tcaaggtata ctttttcgac gtgcttttgt ttttctgaat
     2941 ttgacaaatt ggcaatattg ccttggattt cttcaacaat cattctatgt tcctcctaga
     3001 ataagaagta tctttgagtt aatggtaact cagttgctgc attacttgta attttttctc
     3061 catctacata tacttcatat gtttgtggat caacgtctaa tttaggtgtt gcgctgttat
     3121 ttttcatatc tgctttagat aattgtctaa tatttttaac tggacgcacc atgcgtttta
     3181 aatttaatgc acgattgata ccattttcat aagcagtttt agacacgaat gtcattgacg
     3241 tacttgtaag gtttccgccg tattgaccat acattttacg gtacttcatc ggttcagatg
     3301 taggtataga accatttgca tcaccattta cggcagagtt aattaatccg ccctttacaa
     3361 ctaattcagg tttaacacca aagaaaattg gatcccataa gacaatatca gctagtttgc
     3421 ccggctcgat agatcctaca tattcagaaa taccatgtgt aattgctggg ttaattgtat
     3481 atttagcgat ataacgtttg atacgattat tatcattatg ttcataatca ccatctaaag
     3541 gaccacgttg ttctttcatg cgatgtgcta cttgccatgt tcgtgtaatt acttcaccta
     3601 cacggcccat tgcctgtgaa tcggaactaa tcatactgaa tacacccata tcttgcagaa
     3661 catcttcagc tgcaatcgtt tctttacgaa tacgtgaatc tgcgaatgcg atatcttcag
     3721 gaatagccgc atttaaatgg tgagtaatca ttaccatatc taaatgttca tctacagtat
     3781 tatgtgtata aggcaaagtt ggatttgtag atgaaggtaa aatatttgga aatgcagcgg
     3841 atttaattaa atcaggcgca tgaccgccac cagcaccttc agtatggtac atatgaagta
     3901 cacggtcttt aacagcagcc attgtgtctt ccataaatcc tgcttcattt aaagtatctg
     3961 catgtaatgc aatttgaaca tcaaattcat cagcaacatc taatgcatga ctcaaagcag
     4021 atggtgttgc accccagtct tcatgtactt ttaatccaat tgctccggca ttgatttgtt
     4081 caatgagtgc agttggattt gttgcttgtc ctttacctgt aaaaccgaca ttaatcggta
     4141 aaccttcggc agcttctaac attctatgaa tatgccatgg acctggagtt acagtagttg
     4201 ctttagaacc ttctgaagca ccagtaccac caccaatatg agtcgtaata ccactttcta
     4261 atgcgacttc tgcttgttca ggattaataa aatgaacatg agtatcaata ccaccagcag
     4321 tgacgatttt accttcagcg gcaatgatat ctgttgttga acctataata atgtcgacat
     4381 tatccattat atctgggttg ccggcattac ctatggcgaa aatataacca tttttaatgc
     4441 ctatatcagc tttaaccact ttatcgtaat cgataataac ggcattagaa atgacaaggt
     4501 ctgcaacgtt cacatcatca cgtgttacac gaggattttg cgccataccg tctctaatag
     4561 atttaccacc accaaaagta gcttcttcac cataaaccgc atagtctttt tcaatttgag
     4621 caaatagatt cgtatcacct aaacgaatag aatctccaac agttggaccg tataagctcg
     4681 tatattgatt ttgcgtcatt ttaaagctca tgatcttttt cctccttttt tattcacgtt
     4741 ttcagtaccg ttatctccga atacgcctgc atattcatca ttttcatcag ttgggcgata
     4801 gacacgtgac tcatcgatag gaccattgac cataccacga aaaccaaaaa ttttacgttt
     4861 gccagcatat tcaactaatt gaacttcttt tttatcccca ggttcaaatc gaacagctgc
     4921 tccagctgga atatctaaat gttttccata tgccatttca cgttcgaaat ctaatgctgc
     4981 atttgcttca taaaaatgaa aatgtgagcc cacttgaatt ggtcgatctc ctgtattttc
     5041 aacttcgata actgtttcag gatgatggtt attaatttca acctctgtac tttttgtaat
     5101 aatttctcct ggtatcattt gactgcctcc tttaaacaat agggtgatgt actgtgatta
     5161 acttagtacc atcggggaac gtagcctcga tttcgatatc tgtaatcatg tgttcgacac
     5221 catccatgac atcttctttg tttagaattt gtctaccata actcattaac tctgcaacgg
     5281 tcttaccatc gcgtgcacct tctaataatt catcgctgat taaagctaat gcctcaggat
     5341 gatttagttt caaaccacgt gctttacgac gacgtgcaac ttccgccgcc actacaatca
     5401 ttaatttgtc ttgctctcgt tgtgtaaaat gcaaattaaa acccccaatt tcatattaga
     5461 tacaatttac aaaatttata ttaatcctaa ttgttgtgat aaacaagtaa tatacaaagt
     5521 ttaatacgga acagcaaaaa ataaatttac tatttcgtat ttaaaaatat atgattatga
     5581 ttaaaacgaa tagcaaaata tatttaggat ttggaaaata gagtttttaa tataactttt
     5641 tgtaaaatgg tagtatatta atgagtaaac attcaagaaa aagggtgggc attttgaaaa
     5701 tgatagatgt gcttttgaaa aatatatctc aagtagtgtt aattagtaat aagtggactg
     5761 gtttatttat cttgattgga ttatttgtgg ccgactggac agttggatta gcagctatga
     5821 taggtagtat catagcttat gcatttgcac gttacattaa ttatagtgaa gcagagatta
     5881 atgatgggtt agccggattt aatccagtgt taactgccat tgcgttaaca atctttttag
     5941 ataagtcagg attagatatt gttataacaa tgatagcaac tttattaaca ttaccagtcg
     6001 ctgctgcagt gagagaagtt ttaagaccat ataaagttcc gatgctgacg atgccttttg
     6061 tcattgtgac ttggtttaca attttacttt caggacaggt taaatttgta gatacatcgt
     6121 taaagttaat gcctcaaaac attgaaacgg ttaattttag caacaatgat cgaatacatg
     6181 tcattcagtc attatttgaa ggattcagtc aagtatttat cgaagcgagt gtaattggtg
     6241 gtgtatgtat tttaatcggc atattaatag catcaagaaa agcagcactc ttagctgtta
     6301 tagctagttt gttaagcttt atcattgtag ctctattagg tggtaattat gatgatatta
     6361 atcaggggtt attcggttat aactttgtat taatggcaat cgcactagga tatacattta
     6421 aaacagcgat taacccttat atttcgactt ttttaggtgt gttattaact gtagtggtgc
     6481 aactaggtac aacaacattg cttgaaccgt ttggcttacc tgcattaaca ttgccattta
     6541 ttattgtgac atggatttta ttatttgcgg gtattaaaca tgacaaagta gatgcttgat
     6601 agttaaatca aacctagtat tgtttgaata tcaccttaaa ccatacagcg aattgtatag
     6661 tttaaggtgt atttttggag aaaattaatt ggatacttaa tcgaattatc agctttcttt
     6721 taattgttca cagcacgttc ctttttattt ttaaagtcga gtgttaaact aggaataaat
     6781 aatttatatt gtgtgatgtg tggggtgact aatatgaatg atatggataa ttccttttta
     6841 ataacaacgg aaattcaaag aaaatggatt gaaaaattca aagtaattgg agatacattt
     6901 aaggctaagg ctgaatataa tgatcaacat agccaatttc catataaaaa tattgaatgg
     6961 ttaattaaag aaggttatgg aaaattaacg ttaccaaaag aatatggtgg tgaaggtgcg
     7021 accatagaag acatggttat tttgcaatca tttttaggcg aacttgatgg tgccacagca
     7081 ttatctattg gttggcatgt gagtgtcgta ggacaaattt atgaacagaa attatggtct
     7141 caagatatgt tggagcaatt tgctgttgaa attaataatg gtgcattagt taatagagca
     7201 gttagtgaag ctgaaatggg tagtccaaca agagggggaa gaccaagtac acatgctgtt
     7261 aaagctgatg atgggtatat tttaaatggt gtgaagacat atacatcaat gagtaaagca
     7321 ctaacacata ttattgttgc tgcttatata gaagaattaa aaagtgttgg ttttttctta
     7381 gtggacagga atttgcctgg tgttgaaatt gccgataatt gggacgtatt aggtatgcga
     7441 gcgacagaaa gtcatgattt aatattaaat gatgtaaaag taccattaaa acatttagtg
     7501 gaaacagaga aaagtaaagc gtcaaatggt tggatattgc atataccaag ctgttattta
     7561 ggtatagcac aagctgctag aaattatgct gtagactttg caatccaaca tagccctaat
     7621 agtatagaag gaacgattgc aacattgcca actgtgcaac aaaatttagg aaaaatggaa
     7681 acgctgttgt tatgtgcgag acaattttta tggagtacag cgaaaggcta tcaacaatat
     7741 acagccgaaa gtcagattag aaatgcgacg agtgcgagta aagtaatggt aatgaaccaa
     7801 ggccttgaag tgattgattt agcgatgcgc attgttggtg ctaaaagttt agaaatgaat
     7861 cggccattac aacgttacta tcgtgacatg cgtgcaggat tacacaatcc acctatggaa
     7921 gatgcagcat atacaaatat tgctaagagt attacagata cattttaaat ttaaaaaggt
     7981 aaatcgatcg ttgattggca taaagagatg aaatttgacg ttcggattta ccttttttgc
     8041 gggaatttat aggtatatta agatattttg ctaaattttt tgcgaaaata aaaaaataaa
     8101 taacactagc tattgtaaat gtatatgcaa tcttgtataa ttttggaata attgataatc
     8161 attttcaata ggaggaaatt atgaaaaaac tattattacc attaataatt atgttattag
     8221 tgttagctgc gtgtgggaac caaggtgaaa aaaataacaa agctgaaact aaatcttata
     8281 aaatggacga tggcaaaacg gtagatattc cgaaagaccc taaacgcatt gcagtagttg
     8341 cgccaacata tgctggtgga cttaaaaaat taggtgcaaa cattgtagct gtaaatcaac
     8401 aagttgatca aagcaaagtg ttaaaagata aatttaaagg tgttactaaa attggtgatg
     8461 gtgatataga aaaagttgct aaagaaaagc cagatttaat tattgtatac tctactgaca
     8521 aagacattaa aaagtatcaa aaagtagcac caacagtagt tgttgactat aataagcata
     8581 aatacttaga acaacaagaa atgttaggga aaattgttgg taaagaagat aaagtaaaag
     8641 cttggaagaa agattgggaa gaaacaactg ctaaagacgg taaagaaatt aaaaaagcaa
     8701 ttggacaaga tgcaacagtg tcattgtttg atgaatttga taaaaaatta tacacttacg
     8761 gcgataactg gggtcgtggt ggagaagtat tatatcaagc atttggttta aaaatgcaac
     8821 cagaacaaca aaagttaact gcaaaagcag gttgggctga agtgaaacaa gaagaaattg
     8881 aaaaatatgc tggtgattac attgtgagta caagtgaagg taaacctaca ccaggatacg
     8941 aatcaacaaa catgtggaag aatttgaaag ctactaaaga aggacatatt gttaaagttg
     9001 atgctggtac atactggtac aacgatcctt atacattaga tttcatgcgt aaagatttaa
     9061 aagaaaaatt aattaaagct gcaaaataat tcagctatat aagccagtga aatgagagtc
     9121 tgaaacatat caatcttttg atattgtatt aggctcttat ttttatagca agaaggttag
     9181 atttttg
//
LOCUS       DAKSQE010000097         9171 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.097, whole genome shotgun sequence.
ACCESSION   DAKSQE010000097 DAKSQE010000000
VERSION     DAKSQE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9171
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.097"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(422..2422)
                     /locus_tag="O0V33_002066"
     CDS             complement(422..2422)
                     /locus_tag="O0V33_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8976688.1"
                     /translation="MKLRLLWHIVRRQFITQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFMIISVISIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDTVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGLVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTIMKLITNDYDLSFNDNSKSQ
                     IERQQTIEHIKRDIQGSVNAKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGSIFSKQSVMITALTVQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKGFYAHEQKKSTISINFNVSGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRISKITRNQMTFLFAIPITVALIHTLISSNIVYTLLNMLG
                     INNHHIFLTCYVLAVIIISFLYMAMYKITSYIYAKVIHQQRN"
     gene            complement(2419..3174)
                     /locus_tag="O0V33_002067"
     CDS             complement(2419..3174)
                     /locus_tag="O0V33_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8976689.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLTVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMKLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLVFQQRITDSLALVNGVSVNI"
     gene            complement(3282..4169)
                     /gene="nsaS"
                     /locus_tag="O0V33_002068"
     CDS             complement(3282..4169)
                     /gene="nsaS"
                     /locus_tag="O0V33_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HCX8976690.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFD
                     EQFNQLYVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            complement(4180..4845)
                     /gene="nsaR"
                     /locus_tag="O0V33_002069"
     CDS             complement(4180..4845)
                     /gene="nsaR"
                     /locus_tag="O0V33_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HCX8976691.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQHQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQSIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            complement(4871..5071)
                     /locus_tag="O0V33_002070"
     CDS             complement(4871..5071)
                     /locus_tag="O0V33_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976692.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTESSNQEPSLQQAKEQKDNKG"
     gene            5269..6693
                     /locus_tag="O0V33_002071"
     CDS             5269..6693
                     /locus_tag="O0V33_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCX8976693.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDG
                     YDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKIAKGADPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLQRLLKKEKDEKSADVEKQTKVLQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            6763..6873
                     /locus_tag="O0V33_002072"
     CDS             6763..6873
                     /locus_tag="O0V33_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976694.1"
                     /translation="MLGAWLTRSWYFLNAKYKPPTNVKITANFFIIQTPF"
     gene            complement(6976..7431)
                     /locus_tag="O0V33_002073"
     CDS             complement(6976..7431)
                     /locus_tag="O0V33_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCX8976695.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            complement(7672..8433)
                     /locus_tag="O0V33_002074"
     CDS             complement(7672..8433)
                     /locus_tag="O0V33_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCX8976696.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNNDTTAKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRNDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYL
                     SRKNVPYQFEDGPGDHDYAYWDEAIKRAITWMVND"
     gene            complement(8549..>9171)
                     /locus_tag="O0V33_002075"
     CDS             complement(8549..>9171)
                     /locus_tag="O0V33_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCX8976697.1"
                     /translation="SDSDSDSDSDSDSDSDSNSDSESDSDSDSDSDSDSESDSESDSD
                     SDSESDSDSDSDSESDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSESD
                     SDSDSDSDSDSDSDSDSDSDSESDSDSDSDSRVTPPNNEQKAPSNPKGEVNHSNKASK
                     QHQTDALPETGDKSENTNATLFGAMMALLGSLLLFRKRKQDHKEKA"
BASE COUNT         3016 a         1576 c         1376 g         3203 t
ORIGIN      
        1 catttaaatc gtcaatttac atatattttt gcgtagtaat caatcgttat cattgttatt
       61 tatcgttaca ttacttcgag tatcagtatg tatttcgggc ttcgtttgat aacgacattt
      121 ctttgtgaca tcgcttcatc agtgtaacta caaatacaat gatttcgtga tgttagttac
      181 ccattttatg tgttgcataa aatatgttgt tataaaacat tttgaatcat tttatataaa
      241 caagctatat atttttgata tttaaaaaat ataacttgtc atacaagtaa aaaaccgata
      301 agacatttta tcttatcggt tgaaaattgt tattgttatt tgtagtattt ttaggttttc
      361 tttttcatat aatatatttc agtgaaaaca catgattgat tgtgatttca ctgaaacatg
      421 gttaattgcg ttgttgatga ataactttag cataaatgta ggaagttatt ttgtacatcg
      481 ccatatatag aaacgaaatt ataatgacag ctagtacgta acatgttaaa aatatatgat
      541 ggttattaat ccctagcata tttagtaacg tataaacaat gttgctagaa attaatgtat
      601 gaatcagtgc tactgttatt ggtattgcga acaagaaagt catttgattt cgtgttatct
      661 ttgatattct cccattatct aaaccaagtt ttttcataat gtcataattg tgctgatttg
      721 cataggcttc tgaaacttgc ttatagtaca tcattagaaa tattccaatg acaagtacaa
      781 ttgataccac gccacctaca aatattagtc cacctgtcag acgatcccaa accattagca
      841 tttctgattt agaatttatt gatgcatcgt actttttcga aagcttttga atttgtgatg
      901 ttgttaattt cgtctttcca gatacattaa agtttatcga tattgtcgac ttcttttgtt
      961 catgtgcata aaatcctttt actttttgta attgattgtt atctttaaca attaaaacca
     1021 tactatcttg aatcatcatc aaatttaacg catgtgcatc aagttgtttc actttaaaga
     1081 cttcattatt aagaccgacc ttagactgct ttttaaaaat aggtacattc gtaatcatac
     1141 ctatactttg attgtctaag tgtaatttgt tttggtgata tcgattataa tcttgcacag
     1201 taagtgctgt gatcattact gattgcttag aaaatatcga gccttcatta ccaatgaagt
     1261 ctattggaat atcatcacta gcctgtttta acttgtaata agcacgattc ttttctaaag
     1321 aagctctaaa taatgtcgtt tcatatactt tgaagtcttt tgcattaacc gaaccctgta
     1381 tatcacgttt aatatgctca atcgtttgtt gacgttctat ttgtgactta gaattgtcgt
     1441 taaatgacaa atcataatca ttggtaatca acttcataat ggtatgattc atatcgcgat
     1501 aggttgttag tgtcattgtg agcgttacaa tcaaaaatgt acacagcaaa gtgattgttg
     1561 caagactgac tgcattcatt ttaagacgta cacgcatccc aactaccaca aaaaaatacc
     1621 gtggatgata gtaaactttt gatacctgct gcaatatact aataattatt tcacttatac
     1681 ctacaaaaaa tgcataagta ccgataataa ctaatcctat gacaatccat attttaaaaa
     1741 aagcaccaaa cgtcgtatct tgttgtaatg caataaaata gcctaaatat agtgctgcgc
     1801 ttcctataac aattaaaacg taacgcaacc atcgtgatat gacactggaa tcggaacgat
     1861 gttgataagt tatcggtcgt tgaaaattaa tactgaacaa gttgaatata agtagcatgc
     1921 ccattaatac agcaatgata atcaaagtaa taaacatcgc tacagtatca aatggataat
     1981 accttaacgt agcaacttca ctgcccatta ttttctgtat aaacaagaaa aatatcgcac
     2041 caagtaagta tccgccggca atactaatga ctgaaattat cataaattgt aagatagttt
     2101 ccatcactac aattaaacgc atacttttct tggtcatgcc caatgtcata aaaatgctaa
     2161 actcttttcg tctttgtgac atcataaagt gatttgcata gaaaataaaa ataaaagtta
     2221 aaagcgccat aaaaaaatta gctatgataa taaatggtac taggaagtca ttcttctgtt
     2281 ttatgtagct atttaaccca attgatacaa gcgtatattc aatcatgaat agtacactta
     2341 ccgctaaaat gaatggaatg attacaagtc gctgcgtaat aaattgacgt cgtactatgt
     2401 gccataacaa tcttaacttc atatattgac acttactcca ttcacaagtg ctaagctatc
     2461 tgttattcgc tgttgaaaaa ctaattgact ttcttcacca cgatatattt catgatatag
     2521 acgcccatct ttaataaaaa tgactcgctc tgcataagac gcatcgatat ttgaatgagt
     2581 taccattaaa attgtctgtt ccaattgatg aatctcttga aataacttca tcaatgcttt
     2641 tgatgttttt gaatccagtg cacctgtagg ttcatcggct agtaaaatcg tcggtttagt
     2701 aactaatgca cgtgcaatgg caatacgttg cttctgaccc cctgagattt cagaaggata
     2761 tttgtttaag aatccctcta aatgtaattg tactgtcaac tgatgtaccc tttgttctat
     2821 atcttttcgt ttagcaccag ctaaaattaa tggcatcatt atattttctt tattcgtcat
     2881 tgttggtaaa agattaaaat cttgaaaaac aaaacctacc atttgttgac gatacaatgc
     2941 tttactttta tttttcatat tatttaaatg tgcgccatcc acaataatgt caccttcagt
     3001 taatccatca aaagaagcaa ttaaatttag tagtgtagac ttccctgacc cagactcacc
     3061 cataattgca acaaattcac cagctccaac tgataaattc atttgattaa gtgctgtcgt
     3121 agcattcaaa cctttaccat aaaccttttt tacatgtttc acttctagca acatggcatg
     3181 cacctcattt ctttgttagt tctatattaa aacaaaaaaa tactaataac atttaaatta
     3241 gtgaaaaaat gttactagta tgcttacaat attgaaagtt tttattcatc tggaaattga
     3301 atcgtaaatg ttgtaccctc attttgttta gatacgactg aaacaggatg gtttgtgtga
     3361 gttgaaattt gttttacgat aaataaacca atcccacttg agttactttg ccgctggcca
     3421 ttataacctg aatagccctt atcaaatatt ttaggcaagt ccgcttcact aataccgata
     3481 ccattatctt ttacgtataa ttgattgaat tgctcatcaa attcaatcca tatatcttta
     3541 cctctcgcat acttaagtgc attatttatt aattgttcta tcattaaaga ggtccatcta
     3601 acgtcagtta atacttcgtg atgacaaggt tcataatgga tttttgtttt ttgatcaata
     3661 aactgtattg aatatttcat aataattggg cgaatgatat tgttaatcga aattttagtg
     3721 acagaaatat cagaagtttc atttaataac tttaaataac taagtgctaa acttgtatag
     3781 ttatcaattt gaataacctc ttgacgaaca cgattcacaa cattaggctc atctctttca
     3841 agtaacagtt gtgctgcagt aatgggtgtt ttcatttgat gtacccatgt taaaaagtag
     3901 ctctctacat catttttata ttcaatttgt tcatttttaa gctgatacaa cgcattttct
     3961 aaattttcaa tttgttgttg ttggcttata gtttttacaa aacttaaata tttaatacct
     4021 atgaatatga gtaataataa taaaataacg ccaagtgcta gtaaatatgc ttctaatggc
     4081 aaatgataca ggtaaaacat taagccaaat aaagcaaaaa taactatgac tattgctatt
     4141 tcctgagtaa tacttttaag aaaggtcatt gcattcaccc tatactttat atccgacatt
     4201 tttctttgta atgataaaat cattaacgcc aatagtattt aattttttac gcaggcgcgt
     4261 catgttaaca gctaatgtgt tatcatctat gaagttttct gattcccaac atttttcaat
     4321 taaagcagtt ctacttacat atttatcttc attttgaaat aataacttta atatttgtaa
     4381 ttcagtcaaa gatagctgta tgctttgttc ttgatacacg acttttgctt catctaatat
     4441 taatgtacaa cctttaaccg tcaatgaatc attagctact gacaagtcat aagttcgtct
     4501 caataatgct tgaattttgg caatcgttaa tgacaagtta aatggctttt cgataaaatc
     4561 atctcccccc atttgtattg ccataatttg gtccatatta tcaatacggg aactaataaa
     4621 tataattggc acattagatg tttttcggat ttcttgacac caatgaaaac catttaacgt
     4681 tggcaaatta atatctagca atacaagctg aggttgatgt tggttaaaga tagacagtat
     4741 atcatcaaat tgttcaacga caataacacc gtaattccat tttttaagtt cagatgctaa
     4801 actttctgct ataacaaaat catcttcaac aattaatatt ttcatctata atcttcttcc
     4861 ttcaattgaa ttatcctttg ttatcttttt gttctttagc ctgctgcaat gacggctctt
     4921 gatttgaact ttccgtgtca tttgtatttt gatttagtgt atttgctttt tctttctcga
     4981 gttgcttttt cttcgtacgt ttacgctctt ctaaaataga caacactata aagaatatga
     5041 ttaataatgg agataacatt actagtatca tttttccacc tcaaattata tttaattcta
     5101 tattttcaca cgcttctatt ctaacgcttt ctataacaaa ttgtcatata tattcatttt
     5161 ttgttattct aaatcactct gtttacatat ttttaactaa aactacataa ttgctcaaaa
     5221 ttcctctttt acactgaaga catagtcata tactcaggag gatgtcttat gttatcagtt
     5281 aaaaaagcca caaaatttgc agttgctaac cttattatcg tgtcaacagt ttttagctct
     5341 gtcacacctg caattgcgca atccgataaa agttctaaag atgaaagttt cgcggttggt
     5401 aatactaaaa atccaaaaaa tgttatcttt ttagttggcg atggcatggg tccatcattt
     5461 aacacagctt atcgttatta taaaaatgat cctcatgcta aagaaatgac tcctactgct
     5521 tttgattcat atttaaaagg cacaaatcgt acatattcaa atgacccaaa acagaatatt
     5581 actgattctg cagctggcgg aacagccttt agttctggtc ataagacata taatggtgcc
     5641 atcggtgttg acagtaacaa gcagaaagta aagacggtgc ttgaacgtgc aaaagaaaaa
     5701 ggtaagtcga ctggtcttgt ttcaacagct gaattaacag atgcaacacc agcagcatat
     5761 gctgcacacg tgacttctag agatgataaa aacgaaatcg ctaaacaatt ttataaagat
     5821 aaaatcaacg gaaagcataa agttgatgtt ttattaggcg gtggcgctaa atatttcggt
     5881 aaaagtaatg gcaatttaga taaaaaattc aaaaaagatg gttacgacct tgccacgaac
     5941 agtaaggagt tatcaaaatc tgataaagat aaagtgcttg gtttgtttgc agataaaaat
     6001 atgccacttg caatagatgc ttctaaagac gaaccatcat tagctgatat gcaacaaagt
     6061 gcacttagta agcttgagcg taataaaaaa gggttctttt taatggtcga aggtgcttct
     6121 atagataaat cagcgcattc taacgatatt acaggcgtta tgtctgaaat ggaaggtttt
     6181 gaaaaagcat tcgatgacgc cattcaatat gctaagaaac ataaagatac acttgtcgtt
     6241 gcaacagcag atcactctac tggtggtcta acaattggta aagataaagg atacgaatgg
     6301 aaccctcaac cgattaaatc gatgaaacac tctggatcat atatgacaga aaaaattgct
     6361 aaaggtgcag accctgaaaa agttattaat gaaggttatg gctttgaatt ttcacaagat
     6421 gatatgaaaa aggttaaaaa agagaataaa aagttgcaaa gactacttaa aaaagaaaaa
     6481 gacgaaaaat ctgcagatgt agaaaaacaa acaaaagtct tacaacacgc aattcagaag
     6541 ccagtaaatg ataaatctta tacaggatgg acttccgatg gacatacagg cgaagatgtt
     6601 aatacttacg cttatggacc tagatcagaa tcatttagtg gtaatatcga aaatacagaa
     6661 agcgcaaaaa taatatttga tatattcaag taaagcataa aaaaattccc gagcactata
     6721 acaaggtgtt cgggaatttt ttatgattta atttagtact caatgttagg tgcttggtta
     6781 acacgttctt ggtatttttt aaatgcgaag tataaaccgc caactaatgt taaaataact
     6841 gctaattttt tcatcattca aactcctttc taagatgtgt tgaaactgag ttcaattaaa
     6901 ttatatgttt ttattataca ctttttgaca tattttttaa atttaagaat gcgaagattt
     6961 tcaacatttc tgatgctagc tttctttttc atctttcaaa tgcttaataa aatcaaagcc
     7021 aggtcgccct ttagccaatg cttccatcac atcataccaa tccgaaccga cttgttgata
     7081 tatattttgc aataattcat cttgcgcctt gttcaattca gcaatgagtt gatcgccttt
     7141 atccgtcgaa tataattttt tgacacgtcg atcagtctct aaaacttttt caataatgag
     7201 accttgctct tttaattttt gaagtgttgc atgtgatcct tgtttagaaa tttctaatat
     7261 ttctaataat gatttaatag taataccagg taatttattg ataaaaaaca aaaaacgatg
     7321 atgctgacga ctcataccat acttctctat aatttcatca gcggtagtaa taaaggtttt
     7381 atatgcaaaa taaaacaaca tatgctcata atcgttttta ttggtcgaca tgatgatgac
     7441 tcctttcaaa gcgttaacct cattataata aaatttataa tgtaagtcat acaaatgatg
     7501 ataaaactat aagctaaatc taagtttcac gagaaacatt tttgtgtatc agcaaattgc
     7561 gccaatcata ttaatacaaa attaatttaa acactcagaa atgttagtcg tgcgtaaata
     7621 gtttgtatca ctctgtgtat ttaatgtagg cacatatttt ccaggaaata attaatcatt
     7681 caccatccat gttatagcac gcttaatcgc ttcgtcccaa tatgcataat catgatctcc
     7741 tggcccatct tcaaattgat aaggaacatt tttgcgtgat aaataatcga taaaatctaa
     7801 attgtcttga tataaaaagt cttgtttacc acacataatg agcaattttg gaatttgttt
     7861 atcttcagct acagctttgt ctagcaagta atacggatca tgttcagttc ctttaacact
     7921 tgaaagattg ccaattatgg cctcttttga aaaatcgttc cactctagat ccattaaatt
     7981 ttgcgcttca aacacagcag ataatggtac agctttggca aatttatccc cttgtgttaa
     8041 tgcaaattta attgtgccat atcctcccat agagtgacct gcaataaaat tgtcattacg
     8101 ctttttggaa agtggaaata tttgatgaac ataatcatat acttccaaaa tataatcata
     8161 atagctatga ccatatgcca tgttagcata tgcgctatga tccacattgg gcataatcac
     8221 agctaatttg tgttcattcg catacctttc tatgcttgta tatctcatat atgtcgtttc
     8281 atcacttgat aatccatgta acaacattaa agtttttaat ggtttagcag ttgtatcgtt
     8341 attaaagaag ctttgatctt ccggtaaaat gactgtcaaa ttttgatgca taccaattgt
     8401 tggtgaatga tagtttaatg aaatataagc catacgtcat gacccctttc taattctact
     8461 ttatcaacat tttacgctta aacaattcac tttaaaatca ttttcaacaa aaaaaccgaa
     8521 tactaatgta ttcggcctaa aaaagtattt acgctttttc tttatgatct tgcttgcgtt
     8581 ttctaaacaa tagtaatgat cctaataatg ccatcattgc accaaataaa gttgcatttg
     8641 tgttttcgct cttatctcct gtttctggta aagcatcagt ttggtgttgt tttgatgcct
     8701 tattagaatg gtttacttca cctttaggat ttgatggtgc tttctgttca ttatttggtg
     8761 gtgtaactct tgaatcggag tcgctatctg aatctgactc actgtctgaa tcggagtcac
     8821 tatctgaatc ggagtcgcta tctgagtccg aatcgctgtc tgagtctgac tcactgtctg
     8881 aatccgaatc gctatctgaa tcggagtcgc tatctgaatc cgaatcgctg tctgaatctg
     8941 actcactgtc tgagtctgag tcgctatctg aatccgaatc gctgtctgaa tctgactcac
     9001 tgtctgagtc tgagtcactg tctgattctg aatcgctatc tgaatctgac tcactgtctg
     9061 attctgaatc gctatctgaa tctgagtcac tgtctgaatc tgactcactg tctgagtttg
     9121 agtcactatc tgaatccgag tcactgtctg aatccgaatc gctatctgaa t
//
LOCUS       DAKSQE010000098         9171 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.098, whole genome shotgun sequence.
ACCESSION   DAKSQE010000098 DAKSQE010000000
VERSION     DAKSQE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9171
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.098"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..240
                     /locus_tag="O0V33_002076"
     CDS             <1..240
                     /locus_tag="O0V33_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCX8976698.1"
                     /translation="SFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHDRGVKGQKETIK
                     SMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
     gene            233..548
                     /locus_tag="O0V33_002077"
                     /pseudo
     CDS             233..548
                     /locus_tag="O0V33_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
     gene            1102..1275
                     /locus_tag="O0V33_002078"
     CDS             1102..1275
                     /locus_tag="O0V33_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976699.1"
                     /translation="MKKFFMFFVCLLTVFLSFNFVNSILFDGSFVFLESFIYSLVFTL
                     VFLGLEGDKEKKK"
     gene            complement(1457..1780)
                     /locus_tag="O0V33_002079"
     CDS             complement(1457..1780)
                     /locus_tag="O0V33_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HCX8976700.1"
                     /translation="MEFYQMPMFNKVLMNHLRLEKYQDLMLISQKDFEVGNSIYLNIL
                     VKDIEEKSQNISQKFIVSQLEEQPWNVKEMTIKDPDGNLITLTQSNISDTDFEKLMKK
                     TSKDY"
     gene            2009..2101
                     /locus_tag="O0V33_002080"
     CDS             2009..2101
                     /locus_tag="O0V33_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCX8976701.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(2403..3224)
                     /locus_tag="O0V33_002081"
     CDS             complement(2403..3224)
                     /locus_tag="O0V33_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002477850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCX8976702.1"
                     /translation="MDSLKVLTEVVKTQSFTKAAENLYTSQPSISRDIKRLELEYDVK
                     IFEFRHSKMSLTSDGEKLFQYALQRERLEQNLRHDLKMQPDIISGDLTIGSSYTYGEY
                     RLSQHLADLAKNYPELYIHVYLNNSDTVLQHIKNNTIDLGIIEKEVQSNIIHSRRIAQ
                     DEIVLIRKKSSSSNKSICFIRERGSGTRVYQENALSQMTSSSYLVEINNTNLIKNMVH
                     ADKGFSIVSKSTLTPYDLEKLEVTNLDIKRYFYLVIHKDKYIDTKLNEVIKNLIS"
     gene            3349..4344
                     /locus_tag="O0V33_002082"
     CDS             3349..4344
                     /locus_tag="O0V33_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCX8976703.1"
                     /translation="MKSLKSKAFIYGLLFTFIIAVISLLGSKLPFLDKIGALTIAILI
                     AILYRHFKGYPEAYRSGIAFSSKRLLKLAIILYGLKLNIYDVIGKGSDLVLIDIGVIL
                     FSIGLMLLLNKYIKGDKNLILLLGIGTGVCGAAAIAAVSPILKSREKDSAISIGIVAL
                     IGTIFSLAYTVIYSIFTISPEVFGVWSGTSLHEIAHVILASDFSGQEALSMGLLGKLG
                     RVFLLIPLSIILILIMRKKSHSENEKKRIDVPYFLLGFVMMALFHTYVSLPHMVMQII
                     DNITTICLLMAMVALGLNVSFKDLKDRAFKPLIAVIIVSICLSTVTFIVAKYFYS"
     gene            complement(4679..5254)
                     /locus_tag="O0V33_002083"
     CDS             complement(4679..5254)
                     /locus_tag="O0V33_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCX8976704.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNAYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLKLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEDEKSDK"
     gene            5521..7566
                     /gene="copB"
                     /locus_tag="O0V33_002084"
     CDS             5521..7566
                     /gene="copB"
                     /locus_tag="O0V33_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase CopB"
                     /protein_id="HCX8976705.1"
                     /translation="MSNNKKHQNHEHHSHQEHENHTSHGNHEHHHHGNFKSKFFISLI
                     FAIPIIILSPMMGVKLPFQISFTGSDWIVLILATILFFYGGKPFLSGAKDEISTKKPG
                     MMTLVALGISVAYIYSLYAFYMNNFSGSSTHTMDFFWELATLILIMLLGHWIEMNAVG
                     NAGNALKKMAELLPNTAVKLIDNNQREEVKISDIHIDDIVEVRAGESIPTDGIIVRGE
                     TSIDESLVTGESKKVHKTHNDDVIGGSINGSGTVQVKVTATGENGYLSQVMGLVNQAQ
                     NDKSKAELLSDKVAGYLFYFAVSIGLISFIVWMLIQNNVDFALERLVTVLVIACPHAL
                     GLAIPLVTARSTSIGAHNGLIIKNRESVEIAQHIDYIMMDKTGTLTEGNFSVNHYESF
                     TDELNNEEILSLFASLESNSNHPLATGIVDFAKGKNISYATPQEVNNIPGVGLEGTVD
                     NKKLKIVNVSYLDKSNFYYNKEQFTNLAQQGNSISYLIHDRQVIGIIAQGDKIKESSK
                     QMVSDLLSRNITPVMLTGDNKEVAQTVAEELGISDVHAQLMPEDKESIIQDYQSNGSK
                     IMMVGDGINDAPSLIRADIGMAIGAGTDVAIESGDVILVKSNPSDIINFLSLSKNTMK
                     KMVQNLWWGAGYNVIAVPLAAGILASIGLILSPAVGAILMSLSTIIVAINAFTLKLK"
     gene            7581..9014
                     /gene="mco"
                     /locus_tag="O0V33_002085"
     CDS             7581..9014
                     /gene="mco"
                     /locus_tag="O0V33_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019169201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-copper oxidase Mco"
                     /protein_id="HCX8976706.1"
                     /translation="MYKKMFTILITLFSIMFMVPNDTFAEGKHNMMDMKENDQKRNDM
                     MDMKSHDERKNLNSSQGKNEITFPKVLDPKKDNNGYKSYTLKAQKGKTEFYKGNFSNT
                     LGYNGNLLGPTLKLKKGDKVKIKLVNNLDENTTFHWHGLEIDGKVDGGPSQVIKPGKE
                     KTIKFEVKQEAATLWYHPHPSPNTAKQVYNGLSGLLYIEDDKKNNYPSNYGKNDLPII
                     IQDKTFVSKKLNYTKTKDEDGTQGDTVLVNGKVDPKLTTKEGKIRLRLLNGSNARDLN
                     LKLSNNQSFEYIASEGGHLEKTKKLKEINLAPSARKEIVIDLSKMKEEKVNLVDNDET
                     VILPIINKEKSTNKDTTPKVDKKIKLEGMDDNVTINGKKFDPNRIDFTQKVNRKETWE
                     IENVKDKMSGMKHPFHIHGTQFKVLSVDGKKPSEDMRGKKDVISLEPGQKAKIEVVFK
                     NTGTYMFHCHILEHEDNGMMGQIKVTK"
BASE COUNT         3198 a         1312 c         1523 g         3138 t
ORIGIN      
        1 tcatttaatt caaggcatga agatatgcat tacatgttag aggacattga ggatgaaata
       61 ttaacttctt taaaaagact taaaaaagct atacatgata ggggagttaa aggccaaaag
      121 gaaacaataa agtcaatgca agcttattta atgcaaacaa ttctaactga acttgaagaa
      181 acacatgccc tttatatgcg ccgtaaaaac atgaaacaat ataatatctt taatcaataa
      241 ttcaaatgat tttataacac tcgaagagac atcaaaatat tccgttaaat catctgactt
      301 tttaactgat gacgacgaaa aaatcaagaa attgtaagtg atttagaaaa aggtttgtat
      361 cgaaaacgta tgttaagtta tggtggattg cttaaacaaa agcataaaat tttaaactta
      421 gacgatgccg aagatggtaa tttgattaat acaagtgatg aagataaaac gacagacgaa
      481 gaaaaaaagc acattcaatt accgcaattt ggaatttcga aaagcaaaat tattatttaa
      541 gacattagtg ataactgatg tcttttttta ttgtttttct gtaaaaaagt gctgtcttat
      601 ttttgtgaca aatgctgtat gtagtgtcac aaaagtgtga cactacagct ttttatgacc
      661 tcacacaaaa acattttcga agccttgaaa tatctggctt tgccagatcc cccattcatt
      721 gatggggaca aatttccctt atgctcttac ggagttttta gagaaaaatt gaaatttatc
      781 gatttttgat aaaaaacgcc ctgcaggaat ttagaaaaac atgggaaagt tttaaagaat
      841 tttatgctca tttttaattt gcatgtaaat gggcagtgtt aaaaaattag tcactggttt
      901 tgctgaaatt tttgtttgtg aaattaccat atatttattt ggctcgtatt ttcgtttgaa
      961 gagcttatat aagttttaga tagaaaacta cgcgatttat cttttaatct ttaaaatccc
     1021 cctttaaaat tcaaaatagg ggcattcatt attttagtaa gtcttgtgat aaagtttgtt
     1081 aaaaaattgg aggagtctat tatgaaaaag ttttttatgt tttttgtttg tttattaact
     1141 gtgtttttaa gttttaattt tgttaattcg attttatttg acggttcttt tgtcttttta
     1201 gaaagtttta tttattcatt ggtttttaca ctcgtttttt taggattaga gggggataag
     1261 gagaaaaaga agtaggaaac gcatttatgc cgagaaaatt tattgatatt gagaagaacc
     1321 cttaaccaaa attgaagacg aatgtcggca tagcataagc tattaagccg actattcgac
     1381 aagtttaggg attgttaagg gttccgaggc tcaacgtcaa taaagcaatt ggaataaagc
     1441 aatatagatt tgctatctaa taatctttag aagttttttt cattaatttt tcaaaatcag
     1501 tatcgctaat atttgattga gtaagtgtga ttaagtttcc atctggatct tttattgtca
     1561 tttctttaac gttccatggt tgttcttcta attggcttac tataaatttt tgtgaaatgt
     1621 tttgtgattt ttcttcgata tcttttacta agatatttaa gtatattgag tttccaactt
     1681 caaaatcttt ttgagaaatt agcattaagt cttgatattt ctctagtctc aaatgattca
     1741 ttagcacttt attaaacatt ggcatttgat aaaattccat tgttttcacc tcttttttta
     1801 tttattgtaa tgtattacgt tacgttaatg tcaaaaagag gtggtttttg aaacgagttt
     1861 cttcttgtct tgatactata aaaaaacaaa gttatataac ataagacgac ttatcggaag
     1921 tagatatgct tgtatattca cagatggtat aataatagag tcgcctatct ctcaggcgtc
     1981 aattgacgaa gagaggaggt gcattacttt gctaatcatt ttcgttcaca tcatagcacc
     2041 agtcatcagt ggctgtgctg ttgcgtatta tacttattgg cttagtaaac gcaacaaata
     2101 attaataggc gactttataa gccagcatcg aaaaaatccc cacactactg caatagtgtg
     2161 gggattggtg cattaattgt taatcatttt cgttatatag attataacac tggttagtaa
     2221 agaaatgcaa agtgatatgt acagtatcag ttacaaggta tcgtttcaat attttgacgc
     2281 taattgtcaa taatttaaag gataatttat gaatgttatg tattgagagt ttatcctttt
     2341 tttatataga ttgtcattag aacgtgcatt aaatttagaa taaataatac aaagatcaaa
     2401 attcatgaaa tcaaattttt aataacttca ttaagcttcg tatcaatata cttatcctta
     2461 tgtatgacta aatagaaata acgtttgatg tctaagtttg ttacttctag cttttctaaa
     2521 tcataaggtg ttaatgttga tttagaaaca atagaaaagc ctttatcagc gtggaccata
     2581 tttttaatta gatttgtgtt gttaatttct actaaataag aggatgaagt catttgtgat
     2641 aacgcatttt cctgatagac tcttgtccct gatccacgtt ctctgataaa acatattgac
     2701 ttatttgaag atgatgattt tttgcgaatt aatactattt catcttgagc aatacgtcta
     2761 ctgtgtataa tattactttg aacctctttt tcgattatac ctaaatcgat agtgttattc
     2821 ttaatatgtt gtaatactgt atcagaatta tttaaataca catgaatgta taattcagga
     2881 taatttttgg ctaaatcagc aagatgttgt gacaatcgat attcgccata agtgtaacta
     2941 ctccctatcg ttaaatctcc tgaaattata tcaggttgca ttttcaaatc gtgtcttaag
     3001 ttttgttcta atcgctcacg ttgtaaagca tattgaaata gtttctcccc atcacttgtt
     3061 aaagacattt tagaatgtct aaattcgaaa atttttacat cgtattccag ttctaatcgt
     3121 ttaatatcgc gacttattga tggctgtgaa gtatataaat tttctgcagc tttagtaaaa
     3181 ctttgcgttt taactacttc agttagaact tttaatgaat ccattaacta ggtctcctga
     3241 catttgaata cctctattat aacaaaaatg ttatgagtaa tatacttaat atttatttta
     3301 cgttataact atgcgatgct aagcttttga taaaggagga gttaaaaagt gaagtcctta
     3361 aaaagcaaag cctttattta tggtttatta ttcacattta tcatcgcagt tataagttta
     3421 ttaggctcaa agttaccatt tttagataag ataggtgctt taacaattgc tatactgatt
     3481 gcgattctat atagacattt taagggttac cctgaagcat atcgttcagg aatcgcattt
     3541 tcgtctaaac ggttactgaa attagctatt atattatatg gattaaagtt aaatatttat
     3601 gatgtaattg gaaaaggtag cgatttggta ttaatcgata ttggagtcat cctttttagt
     3661 atcggattaa tgttgttact aaataaatat attaaaggag ataaaaacct catcctatta
     3721 ttaggtattg gcactggggt gtgtggcgcg gcagctattg cagcagtatc tccaatatta
     3781 aaatctagag aaaaagattc tgcgattagt attggtattg tcgcacttat tggcactata
     3841 ttttcgcttg catatactgt aatatattct atttttacaa tatctcctga agtatttggg
     3901 gtctggtcag gtacaagttt acatgaaatt gcacatgtaa ttcttgcttc agacttctca
     3961 ggccaagagg ctttgagtat gggactgtta ggaaaactgg gacgtgtgtt tttattaata
     4021 cctctgagta ttattttaat tttaattatg agaaaaaaat cgcatagtga gaatgaaaaa
     4081 aagcgtattg atgtacctta ctttttactt ggttttgtca tgatggcatt attccatact
     4141 tatgtatcct tgccacacat ggttatgcaa attatagata atatcactac tatatgctta
     4201 ttgatggcaa tggttgcatt agggttaaat gtttcattta aagacttaaa agatcgtgct
     4261 ttcaaaccat taatagctgt cattattgta tctatttgtt tatcaactgt gacatttatt
     4321 gttgctaagt acttttatag ttagactata agtatattaa aaaattgata tagattctca
     4381 caaacaaaca caacacattg aaggcattcc atattactac ggtcaaaaaa agatcagcct
     4441 ttttcttact aggcctattc ttctttatta ttactgacat atcaagcttt acaatgcata
     4501 ttccgactta ccttgacagt ttaattatag taaatttcaa tatttctatt aactatggaa
     4561 caatataatt tagggtacat tctaatcata caaatgaagt tgttgaattt ataatagctt
     4621 aaacgtatga ttagtgtgta ttttttaact tttattattg ttgaaagtta agttgtattt
     4681 acttatcact cttttcatct tctaatttat tgagataacg ataaattgtt gtacgtgaaa
     4741 cttgccattg ttcagcaatt gtcttaattg gcgtaccatt atcataaagt gttttgagca
     4801 attttaaatc ttgtttgttt aatttttcgg gtcttccacc ataccgtcct ctagcacgtg
     4861 ctgcaattct accagctgaa gaacgttcta aaattaaatt acgttcaaat tctgcaaatg
     4921 ctgcgaataa atgaaataat aattgtccgg tagaacttga tttatccatc gttatatttt
     4981 cttctaaact atggaaactt acacctcgtt cattaagctc attaactaat gtaattaaat
     5041 cctccatatt acgtcctagt ctatctaatc tccaaacaac aatagtatct ccagaccttg
     5101 caaattcaat tgctttatct aaaccaggtc ttttactttt tgaaccactc atatgatcgt
     5161 taaatatctt ttcacaacca tatgcattta atcgatcttc ttgtaaattt aaattttgta
     5221 atcctgttga aactcttgca taacctattt tcaaaataca aacctcctat atccatcatt
     5281 agtgtaccat aacttaaata cgggtacttt tatgaacata gattttttat tgggtttctg
     5341 aacataaaaa taaatgtaat cctcacttat ttcgatataa aaaaagagtg tacagaaaat
     5401 ggttattttc tgtacactac aaaataaaaa agtcaaatgg tatatacccc tatcccgtat
     5461 atgttacact caaattgtct tattactatc tttaaaaata taaaattagg aggtttaatt
     5521 ttgtcgaata acaaaaaaca ccaaaatcat gagcatcata gtcatcaaga acatgaaaat
     5581 cacactagtc atggtaatca tgaacatcac catcatggaa attttaaaag taaattcttt
     5641 atttctttga tttttgcgat accgattatt attttatcgc ccatgatggg agttaaatta
     5701 ccgtttcaaa tttcatttac agggtcagat tggattgtat taattcttgc tacaatttta
     5761 ttcttttacg gaggaaagcc atttttatca ggggctaaag atgaaatttc aactaaaaaa
     5821 cctggaatga tgacgcttgt tgctctaggt atttcagtag cttatattta tagtttatac
     5881 gccttttata tgaataactt cagtggttca tccacacata caatggactt tttctgggaa
     5941 ttagcgactt taattttaat catgttatta ggtcattgga ttgaaatgaa cgcagtaggt
     6001 aatgcaggca atgcactgaa gaaaatggca gaacttttac ctaatactgc agtgaagctg
     6061 attgataaca atcaacgaga agaagttaaa atatcagata ttcatattga cgatattgtg
     6121 gaagtcagag caggcgaaag tatacccaca gacggaatca ttgttcgagg agaaacatca
     6181 attgacgaat cattagttac tggcgaatca aaaaaagttc ataaaactca taatgatgat
     6241 gttattggag gttcaattaa tggttcaggt acagtacaag ttaaagtgac tgctacgggt
     6301 gaaaatggct acctatctca agttatgggt cttgttaacc aagctcaaaa cgataagtca
     6361 aaagctgaac tcctctctga taaagtcgca ggttatttgt tttactttgc agtaagtatt
     6421 ggcctgattt catttatcgt atggatgctt attcaaaaca acgtcgattt cgcacttgaa
     6481 cggctagtaa ccgtattagt cattgcttgt ccacatgcat taggcttggc aataccttta
     6541 gttacagcac gttctacctc tataggcgca cataatggtt taatcattaa aaacagagaa
     6601 tctgtagaaa tcgcacaaca cattgattac atcatgatgg ataaaactgg gacactaact
     6661 gaaggtaact tttcagtgaa tcactatgaa agttttacag acgagttaaa taatgaagaa
     6721 atattgagtc tatttgcttc attagaaagt aattctaatc atccacttgc cactggtatt
     6781 gttgattttg ctaaaggcaa aaatatttca tatgctactc ctcaagaagt taataatatt
     6841 ccaggtgtag gcttagaagg cacagttgat aataaaaaac tcaaaatagt gaatgtttct
     6901 tatctcgata aaagcaattt ttattataat aaagaacaat ttacgaatct cgctcaacaa
     6961 gggaattcta ttagttatct aattcatgac cgacaagtta ttggtattat cgcacaaggc
     7021 gataaaatta aagaaagttc aaaacaaatg gtttcggatt tattatcgag aaacattacg
     7081 ccagtcatgc ttacaggcga taataaagaa gtggcacaaa cagtggctga agaattaggt
     7141 attagtgatg tccacgcgca attgatgccc gaagataaag aaagtattat ccaagattat
     7201 caaagcaatg gtagcaaaat catgatggtt ggtgatggta tcaatgatgc gccaagcctt
     7261 attagagcag atattggtat ggcaattggc gcagggacag atgtagctat tgaatcgggg
     7321 gatgtcatac tcgttaaaag taatccttct gatattatca attttttgtc cctttcaaaa
     7381 aatacgatga aaaaaatggt tcaaaattta tggtggggcg caggatataa cgtgattgca
     7441 gttcctcttg cagctggtat attagcttct attggtttaa tattatcacc tgctgtgggt
     7501 gcaatactta tgtctttaag tacgatcatc gtagcaatta atgcatttac attaaaatta
     7561 aaataaaaga ggtaaatata atgtataaaa aaatgttcac aattttaatt acgttatttt
     7621 ctataatgtt tatggttcct aatgatactt ttgcagaagg taagcacaac atgatggaca
     7681 tgaaagaaaa tgatcaaaag cgaaatgata tgatggatat gaaaagtcat gacgaaagaa
     7741 aaaatttgaa ttcttcacaa ggaaaaaatg aaataacatt tcccaaagtt ttagatccta
     7801 aaaaagataa caatggttat aaaagttata cattaaaagc gcagaaagga aagacagaat
     7861 tttacaaagg taatttctct aacactttag gatacaatgg aaatttactt gggccaactt
     7921 taaaattaaa aaaaggagat aaggttaaaa ttaagttagt caacaactta gatgaaaata
     7981 caacatttca ttggcatggg ttagaaatag atggaaaagt ggatggaggc ccttctcaag
     8041 ttataaaacc aggaaaagaa aaaacaataa aatttgaggt taaacaagag gctgctacat
     8101 tatggtatca tccccacccc tccccaaata cagctaaaca agtttataat ggtttatcag
     8161 ggttattata tatagaggat gacaaaaaga ataattatcc tagcaattat gggaaaaatg
     8221 atttgcctat aataatccaa gataaaacat ttgtatctaa aaagttaaat tatacaaaaa
     8281 cgaaagatga agatggtact caaggtgata cagtccttgt gaatgggaaa gtagatccta
     8341 aattaacaac aaaagaagga aaaatacgtt tgagactttt aaatggctcc aatgctcggg
     8401 atttaaatct taagctaagt aataatcaaa gttttgaata tattgcttca gaaggcggcc
     8461 atttagaaaa gactaaaaag ttaaaagaaa ttaatttagc tccttcagca agaaaagaaa
     8521 tagtaataga tttatctaaa atgaaagaag aaaaagtcaa tcttgttgat aatgatgaaa
     8581 cagtaatttt accaattatt aacaaagaaa aaagtactaa caaagacact actccaaaag
     8641 tagataaaaa aatcaaatta gaaggtatgg acgataatgt tactattaat ggcaaaaaat
     8701 tcgatcctaa cagaatagat ttcacacaaa aggtaaaccg taaagaaact tgggaaattg
     8761 aaaacgtcaa agataaaatg agtggtatga agcacccgtt ccatatccac ggaacgcaat
     8821 ttaaagtttt atcagtggat gggaagaaac cttcagaaga tatgagaggc aaaaaagacg
     8881 ttatatctct ggaacctgga caaaaagcca aaatagaagt ggtttttaaa aatactggga
     8941 catacatgtt ccattgtcac atacttgagc atgaagataa tggaatgatg ggacaaataa
     9001 aagtaacaaa ataatttata tcgtcgcgat cattttatca tctccaaata ttttagggat
     9061 gaggctggga cattaagttc ttaggcaatg taaaaaagct gatttctatt aattatttga
     9121 tggaaatcag cttttttgat atttatttta taatgtacag ctcgttgagc t
//
LOCUS       DAKSQE010000099         8946 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.099, whole genome shotgun sequence.
ACCESSION   DAKSQE010000099 DAKSQE010000000
VERSION     DAKSQE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8946
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.099"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..478)
                     /locus_tag="O0V33_002086"
     CDS             complement(<1..478)
                     /locus_tag="O0V33_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001800370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCX8976707.1"
                     /translation="MLYNTDNKKFKFLPNWLETTSYWFNMEHNNNINKKYKKFSRGSI
                     IYLDFGINIGHEFSGRHFGIVLNKNDSPYNSLVTVVPLSSKNKKHYLPLQNIVFKTSL
                     SILNKTVDNIEFDLDVINHVIFTVIEIINKNNLQNTLEQNTIDYLDDFAIKTRFPTE"
     gene            complement(516..1730)
                     /locus_tag="O0V33_002087"
     CDS             complement(516..1730)
                     /locus_tag="O0V33_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCX8976708.1"
                     /translation="MWVREITKNNSTAYRYLERYTDPLTGKYKTVSVTRNKNNVRSQK
                     DAQLELNKIIEQRLKHYSTKQLENLTFHDACDEWLEHYKTHSGSKPTTIKEKKSNTNT
                     VKNAIDSKVLISKITHTYLQNIINEWAKSHSIGHVQSLVIVIRSVFKYAFKYYDLHDI
                     SVLDKIDIPKKAQTRNEFQAKRNNYLEDSEVKELLECFDYLIKHKRHATRKRNYEMVK
                     ALVEFQINNGMRIGELLAIKTDNVDVENKTLEIDGTINWVTDVVTGAFGVKETTKTSK
                     SYRTIGLTAQSINLLKKLMLENKKENQWNDKFIDRGYIFTNTAGSPIDLNKVNNIIKE
                     ATDISSINKRVTTHTLRHTHISTLAQLGINLKAIQERVGHSDYKTTLEIYTHVTDKMA
                     KDMMNKLEGIGS"
     gene            complement(2186..2881)
                     /locus_tag="O0V33_002088"
     CDS             complement(2186..2881)
                     /locus_tag="O0V33_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCX8976709.1"
                     /translation="MKFGDILKEYRKQLKLSVNQLSKLSNVSVGYISKLENNKRKFPT
                     TRTLFLLLLGFKNSKINDQSKSIQDVDNEIKYILNEFINAEDSEINSEDLENLYKDFN
                     TFYEDLHKKVGNKDERNRKKNIFVYEDDKKKKHSVNLERPINDIAFHLKDNANQKFYN
                     GVMLNEYDKNMINEIINSFLVTKLSQEQVDLNEDIEQLQKDFNDFRKESMLNKKQLKM
                     FAIHNNIQSLKDK"
     gene            3025..3237
                     /locus_tag="O0V33_002089"
     CDS             3025..3237
                     /locus_tag="O0V33_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCX8976710.1"
                     /translation="MKNNLSMIMGRKRITATKLSEDTGVSRTTIHELYHEKQINPSFS
                     TVSKLCKSLDVTLNEFFGINEKEGVK"
     gene            3238..3510
                     /locus_tag="O0V33_002090"
     CDS             3238..3510
                     /locus_tag="O0V33_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCX8976711.1"
                     /translation="MSRTKLQDFPSKENTVTEPKQVVVNPLFAKPNALAGIFGISYSS
                     VNRILKEWEKDSKVVDDLYYSLSSTLIVISIPRFEEYMKVRHKKWM"
     gene            3522..3668
                     /locus_tag="O0V33_002091"
     CDS             3522..3668
                     /locus_tag="O0V33_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCX8976712.1"
                     /translation="MKIYLTYICLVSLLTILLLAISNMYVAFSVYGIMVTYGFNLTGG
                     LENE"
     gene            3661..3870
                     /locus_tag="O0V33_002092"
     CDS             3661..3870
                     /locus_tag="O0V33_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCX8976713.1"
                     /translation="MNNEQKEVIEHLVYQLELSVMNNLESYEHTEYVNGIEVVSEISR
                     EKHLELIMKWCAQELKNNFQLEKGE"
     gene            3873..4196
                     /locus_tag="O0V33_002093"
     CDS             3873..4196
                     /locus_tag="O0V33_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1474 family protein"
                     /protein_id="HCX8976714.1"
                     /translation="MNWEIKDLMCDIEVIKQKINDVATKHAWFVEDRFVKNELETKRE
                     HINFSASYLEHRIQNEHTVELLQVYLKEFDELIQKFHEIEKASSDVSLATESDDAKNS
                     IKVAE"
     gene            4261..5130
                     /locus_tag="O0V33_002094"
     CDS             4261..5130
                     /locus_tag="O0V33_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HCX8976715.1"
                     /translation="MNEIKLEYDTHVSVVHYESLDSRSFNSFSKPKWSKLVNKLSVPI
                     EANYKYARGVAVYGDIKDGANDHGEIIKKHRNDKNVIYRNVIVLDYDEINDLKQLHDA
                     ISSVLSSVAWYWHTSFSHTTEQARIRLYIPLNERISADDYCKYTKVLANKIGHKVDEG
                     SYQPSRCFALPVIQKGHIFIKRVNDCPIMDVDMLEQWSKEYKQSNGSPNIKGYTRRDS
                     AYWRDIAFGVSEGERNSTLASITGYLLRRYVDPNLVYGLVSAWASVCKPPINQSEVNN
                     TFKSILKKDSKNS"
     gene            5147..6616
                     /locus_tag="O0V33_002095"
     CDS             5147..6616
                     /locus_tag="O0V33_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein E"
                     /protein_id="HCX8976716.1"
                     /translation="MEDVTNEEVFEMIDSRTGVLNANDWKSQLRRSATTQALKKTTTN
                     AEIILCNDESLKGLVQYDTFEKVTKLKRLPYWRSKGDTNYYWADIDTTYVISHIDKLY
                     NVQFSRDLIDTVIEKEAYQNRFHPIKSMIESKSWDGIKRIETLFIDYLGAEDNHYNRE
                     VTKKWMMGAVARIYQPGIKYDSMIILYGGQGVGKSTAVSKLGGHWYNQSIKTFKGDEV
                     YKKLQGSWICEIEELSAFQKSTIEDIKGFISAIVDIYRASYGKRTERHPRQCVFVGTT
                     NNYEFLKDQTGNRRFFPITTDKNKATKSPFDDLTPDVVQQMFAEAKVYFGENPTDKAL
                     LLDKEASEMALKVQEAHSEKDALVGEIEEFLERPIPSDYWYRTLEEKRVSAHDVIDQD
                     YIKLYGDGKLIELPNAKPGAYVWRDKVCSMEIWKVMMKRDDQPQQHHLRKIDKALRNT
                     SYCGQSKSRHRFGEGIGRQYGFGINLISYYQGLKSKEQK"
     gene            6902..7264
                     /locus_tag="O0V33_002096"
     CDS             6902..7264
                     /locus_tag="O0V33_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012990923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCX8976717.1"
                     /translation="MNKNQLKSEILEYIKAHAGTSFVEIERVFEENNFDYKGDGAYTS
                     GQHPNVVFWIGWNQEAFDVIAELKKDRRIEMDICEPIVYMVDGKGLDLPIVRSKNIKT
                     DHWLPVTFTISKKETECV"
     gene            7266..7550
                     /locus_tag="O0V33_002097"
     CDS             7266..7550
                     /locus_tag="O0V33_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCX8976718.1"
                     /translation="MNDKEKIYNQLHHDAPIQIMPAPENLFVEYIEDGEVWYSPVVCM
                     ALNKAHNINFYDSDDVGCIDKAGTFSIKKFNPETGEFEQFSKMAQKEVTQ"
     gene            7547..8188
                     /locus_tag="O0V33_002098"
     CDS             7547..8188
                     /locus_tag="O0V33_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012990921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCX8976719.1"
                     /translation="MNIETIVNQFETRAGTLLRYYTGLLEHSKVQPYCFKLYNDPFDM
                     VYVMMNSKLFGHVYIKDCKVRQSFELASPKHTEGLIRSIEGHYVGYELHDGKHLSISD
                     MMASHLFEDEYFMYGLQTYAESNNSDVFEYLENGFDTDTLEGIQSSNTDVIANIEMLY
                     QLATGINEPAPELVEGLKLVTEFVQDENATQEDYKALERKLTELKSSYYSLNK"
     gene            8724..>8946
                     /locus_tag="O0V33_002099"
     CDS             8724..>8946
                     /locus_tag="O0V33_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCX8976720.1"
                     /translation="MQSIAEKETYHLPTEHLQVFNVIKNTSNKYITKTKILNQLGYEY
                     NSSNERWLRRVINSLVYDYGYPIGCSYKPS"
BASE COUNT         3076 a         1193 c         1637 g         3040 t
ORIGIN      
        1 tttctgtagg gaagcgtgtt ttaattgcaa aatcatctaa ataatctata gtgttttgtt
       61 ccaaagtgtt ttgtaaatta tttttattaa taatttctat aaccgtaaaa attacgtgat
      121 ttataacgtc taaatcaaat tcgatattat caactgtctt gttcaaaatt gataatgatg
      181 ttttaaatac aatattttgt aatggtaaat agtgtttttt attttttgaa cttaatggta
      241 caactgttac taaagaatta tatggactat catttttgtt taatactatt ccaaaatgtc
      301 tgccagaaaa ttcatgccca atattgatgc caaaatctaa atatataata gatccccttg
      361 agaatttttt gtattttttg tttatattgt tattatgctc catattaaac caatatgatg
      421 ttgtctctaa ccaatttggt aaaaatttaa actttttatt atctgtgtta tataacagaa
      481 taaatactca cagctttatt tattctgtta tccatctaac tccctatccc ctctaattta
      541 ttcatcatat cttttgccat cttatcagta acatgtgtgt atatctctaa ggtggtttta
      601 tagtccgagt gacctacacg ctcttgtatc gcttttaggt taattcctaa ttgcgcaagt
      661 gtagatatgt gtgtgtgacg taatgtgtgc gttgtcacac gcttgttaat tgaacttata
      721 tcagtagctt ctttaataat attattcacc ttatttaagt caatagggct accagcagtg
      781 ttagtaaata tataacctct atctatgaat ttatcattcc actgattctc ttttttattt
      841 tctagcatga gttttttaag taaattaata ctttgagctg tgaggcctat tgttcgataa
      901 ctcttactcg tcttagtcgt ttctttcact ccaaatgctc cagttactac atctgtaacc
      961 cagttaattg tgccatcaat ctctagtgtt ttattctcca cgtctacatt gtctgtcttg
     1021 attgctagga gttcgccaat gcgcattcca ttgttaattt gaaattctac taatgctttt
     1081 accatttcat agttacgttt acgcgtagca tgacgcttat gtttaattag atagtcgaag
     1141 cactctagta actcctttac ttcgctatct tctaaatagt tattacgttt agcttgaaac
     1201 tcgtttctgg tttgggcttt cttaggtata tctattttat ctaacacact aatatcgtgc
     1261 agatcataat atttaaacgc atatttgaaa acggaacgaa taacaataac aagagattga
     1321 acatggccaa tactatgtga tttagcccat tcattaatga tgttttgtaa gtaggtgtgc
     1381 gtaatcttgc tgatgagtac tttgctatca atagcatttt taactgtatt agtattactt
     1441 ttcttttctt taatagtggt tggtttcgag cctgaatgtg tcttgtaatg ctctaaccat
     1501 tcatcgcacg catcatggaa cgttaagttt tcaagttgtt tcgtactgta atgtttcaaa
     1561 cgttgttcaa ttattttatt taattctaat tgagcgtcct tttggctacg tacattattc
     1621 ttgttacgtg taactgatac tgttttatac ttgccagtta aagggtctgt atagcgctct
     1681 aaatagcgat aggccgtact attgtttttg gtgatttcac gaacccacat ttgtcatccc
     1741 tccttgtcat cttcgtcgct tttttctctg tagtttttta gatggtcgta ttttttcatt
     1801 gagatattta atatgtataa agtattaatg atggctatta gcaatataat gataaagaca
     1861 ataattacta aactttcgaa tttaaaaggt aatttgaaca aaaaaatacc ctaataatcc
     1921 taaatcttat acttatcgaa tttcataaca tatctccaat gaaagttatt tttttgaata
     1981 aaaagctcat ttattcgttt ttgtatttaa aaaattggaa ttcagcaacg cttatgcgag
     2041 caataattca aagtttttat cgaaaaaaac atgtttattt tgtgttttgg tgtatttggt
     2101 gttcatatat gagtgcgcca aatttagtat caccttatcc atttgttaaa tttcattatt
     2161 ttcccctttg tttactctta ttttattatt tatctttaag tgattggatg ttgttgtgaa
     2221 tagcaaacat ttttaattgt tttttattta acatagattc ttttctgaaa tcattaaaat
     2281 ctttttgtag ctgttcaata tcttcgttta aatctacttg ttcttgcgat aatttagtaa
     2341 ctaaaaacga attaatgatt tcatttatca tatttttatc atattcattt aacattacac
     2401 cgttataaaa tttttgattt gcattatctt taaggtgaaa agctatatca tttataggtc
     2461 tttctaaatt aacagagtgt ttctttttct tatcatcctc atatacaaat atattctttt
     2521 ttctatttct ttcatcttta tttccaactt ttttatgcaa atcttcatag aaagtattga
     2581 aatctttgta taaattttcc agatcttcac tatttatttc gctatcttca gcatttataa
     2641 actcatttaa aatatatttg atttcattgt ctacatcttg tattgattta gattgatcat
     2701 ttatttttga gtttttaaat cctagtaata ataaaaacaa tgttctagta gtcgggaatt
     2761 tccttttatt attttcaagt ttactaatat agcctactga tacgtttgat agtttagaaa
     2821 gttgatttac agataacttt aattgttttc tatattcctt taatatatct ccaaatttca
     2881 taaaatcacc tctatttcat tatacaacaa ttatagggat taattccaaa gaataaaagt
     2941 attgactgtg aaatatgatt cgtggtacat tctttttgta ggcaattcct aaaaatatga
     3001 aagcctacaa atggaggcga taatatgaaa aataatttaa gtatgattat ggggcgaaaa
     3061 aggattacag ctacaaaatt gagtgaagat accggtgttt ctagaactac aattcatgag
     3121 ttgtatcacg aaaaacaaat taatccaagt tttagtactg tttcgaaatt gtgtaaatcc
     3181 cttgatgtaa ctttgaacga attttttgga atcaatgaaa aagagggggt caaataaatg
     3241 tctagaacaa aattgcaaga ctttccatca aaagaaaata cagttacaga accgaagcaa
     3301 gttgtagtaa atccgttgtt tgcgaaacct aatgcactag ctggtatttt tggaatttca
     3361 tacagttcgg tgaatcgcat tttaaaagaa tgggaaaaag attctaaagt tgttgatgat
     3421 ttatattatt cactatcatc aacattgatt gttatcagta ttccgcgatt cgaggagtac
     3481 atgaaggtgc gtcataaaaa atggatgtag gaggtaaagc aatgaaaata tacttaactt
     3541 atatctgctt agtttcattg ttaacaattt tattactagc aatatctaac atgtatgtcg
     3601 cttttagtgt atacggcata atggtaactt atggatttaa tttaacagga ggattagaaa
     3661 atgaataatg aacaaaaaga agtaatagaa cacttggttt atcaacttga gttaagtgtc
     3721 atgaataatt tggaaagtta cgaacacaca gaatatgtta atggtattga agtggtttca
     3781 gagatcagtc gtgaaaagca cttagaattg ataatgaaat ggtgtgcaca agaattaaag
     3841 aataattttc aattagagaa aggagaataa aaatgaattg ggaaattaaa gatttaatgt
     3901 gtgacattga agtgataaaa caaaaaatta atgatgtagc taccaaacat gcttggtttg
     3961 ttgaagatag atttgtaaaa aatgaattag aaacaaaacg ggaacatatt aatttttctg
     4021 ctagctattt agaacatcgt atacaaaatg aacatacagt tgagttatta caggtttatt
     4081 taaaagagtt cgatgaactt atacaaaaat ttcatgaaat agaaaaagca tcatctgatg
     4141 taagtttggc gacagaatca gatgacgcaa agaattcaat aaaagtagca gagtaatata
     4201 gaaattacac attcttatta taacatcttt gctctgttgt ttcattaaga ggtgcaaaaa
     4261 atgaatgaaa ttaaattaga atatgacaca catgtttcag tggtacatta tgaaagttta
     4321 gactcacgtt catttaatag cttttcaaaa cctaaatgga gtaagttggt taataaactg
     4381 tctgtaccta tagaagcgaa ttataagtat gcacgtggtg ttgctgtgta tggtgatatt
     4441 aaagacggtg caaatgatca tggtgaaatt atcaaaaagc atcgaaacga taaaaatgtc
     4501 atatatagaa atgtgattgt acttgattac gatgaaataa atgatttaaa acaactacat
     4561 gacgcaatca gctcagtttt aagcagtgtt gcatggtatt ggcacacatc gtttagccat
     4621 acaactgaac aagctagaat acgcttgtat atccctctaa atgagcgaat aagtgcagat
     4681 gattattgta aatatacaaa agtattagca aataaaattg gccataaagt ggatgaaggt
     4741 tcgtatcagc caagtagatg ttttgcgtta ccagttattc aaaaaggaca catatttatt
     4801 aagcgagtga atgactgtcc aattatggat gttgatatgc tcgaacagtg gtcaaaggag
     4861 tataaacaat caaatggtag tcctaatatc aaagggtaca cacgacgtga tagtgcgtat
     4921 tggcgagata tagcttttgg tgtaagtgag ggagagcgca attcaacatt ggcttcaatt
     4981 acaggttatc ttttgcgtag gtatgtagat ccaaacttag tttatgggtt agtgagtgcg
     5041 tgggcgagtg tatgcaaacc acctattaat caaagtgaag taaataatac ttttaaaagt
     5101 attttgaaaa aagatagtaa aaacagttag aaatggaggt ttttgtttgg aagatgttac
     5161 aaacgaagaa gtatttgaaa tgattgatag cagaaccggt gttttaaatg ctaatgattg
     5221 gaaaagtcaa ttaaggcgtt ctgctactac acaagcattg aaaaaaacga ctacaaatgc
     5281 tgaaatcata ttgtgtaatg atgagagttt aaaagggcta gtacaatatg acacttttga
     5341 aaaagtaacc aaactgaaac gtctaccgta ttggaggtca aaaggagata cgaattatta
     5401 ttgggctgat atagatacca catatgtgat ttcacatatt gataaattgt ataatgtgca
     5461 gtttagccgt gatcttattg atactgtaat tgaaaaggaa gcttatcaaa atagattcca
     5521 ccctattaaa tcgatgattg aatctaaatc atgggacgga atcaaaagaa ttgaaacgct
     5581 cttcattgat tatttaggtg ctgaagataa ccactacaat agagaagtta caaaaaaatg
     5641 gatgatgggt gcagttgcta gaatctatca gccaggtatt aaatatgatt ccatgattat
     5701 tttatatggt ggtcaaggtg ttggaaaatc tacggcagtg agtaaattgg gaggtcattg
     5761 gtataaccaa agtattaaaa cgtttaaagg tgatgaggtc tataagaaat tgcagggttc
     5821 ttggatatgt gaaattgaag aactgtcggc atttcaaaag tctactattg aagatattaa
     5881 ggggtttata agtgctattg tagatattta tagagcttcg tatggtaaac gaacagagcg
     5941 tcatcctaga cagtgtgtgt ttgtagggac aaccaataac tatgagtttt taaaagacca
     6001 aacaggcaat cgtcgttttt tccctattac gacagataaa aataaagcaa ctaaaagccc
     6061 gtttgacgat ctaacaccag atgttgtgca acaaatgttt gccgaagcta aagtatattt
     6121 tggtgagaat ccgacggata aagcattgtt gctagataaa gaagcgagtg aaatggcttt
     6181 aaaagtccaa gaagctcatt ctgaaaaaga tgctttagtt ggagaaatag aagaatttct
     6241 tgaacgtcct attccgtcag actattggta tagaacgtta gaagaaaaaa gagtgtctgc
     6301 gcatgatgtt atagaccaag actatattaa attatatggt gatggtaaat tgattgaatt
     6361 accgaatgca aaaccaggtg cttatgtatg gcgtgacaag gtatgtagca tggaaatttg
     6421 gaaagtgatg atgaaacgag atgaccaacc acaacaacac catttaagaa aaattgataa
     6481 agcgttaaga aatacaagtt attgtgggca aagtaagtcg cgtcatagat ttggtgaagg
     6541 tattggtaga caatatggtt ttggtattaa tttaatatcc tattatcaag gtttaaaaag
     6601 caaagaacaa aaataacggg acaacgggac gattatggga caatggtagg acaccttcaa
     6661 tctcttgtgg cagtaagcat tatgttacgt ttgtccctgt gtcccgcaac ttttacccta
     6721 aacttttaaa gtaatatata cacattaaaa aatatataag tgtaggcata aaacagtggg
     6781 acaatgggac agataacttt aatccattgg gagagtggtg tttgagcatt gtcctgacaa
     6841 tgtcctgaaa catattgaaa atagcgaaat gggacaccta tcaaaaatta ggaggaagaa
     6901 aatgaataaa aatcaattaa agtcagaaat tttagaatat ataaaggcgc atgctggtac
     6961 atcatttgta gaaatagaac gtgtatttga agaaaataac tttgattata aaggtgacgg
     7021 cgcatataca agtggtcaac atcccaatgt tgtgttttgg attgggtgga atcaagaagc
     7081 gtttgatgtt atcgctgaac ttaaaaaaga cagacgtatt gagatggata tttgtgagcc
     7141 aattgtttat atggttgatg gtaaaggttt ggatttgcct attgtaaggt cgaaaaacat
     7201 taaaacagat cattggctac ctgtcacgtt tactattagt aagaaagaaa cggagtgtgt
     7261 ctaatatgaa tgacaaagag aaaatttata atcaacttca tcatgatgca ccaattcaaa
     7321 ttatgccagc acccgaaaat ttatttgtcg aatatataga agatggcgaa gtgtggtatt
     7381 caccagttgt atgtatggct ttaaataaag cgcataatat taatttctat gatagtgatg
     7441 atgtgggatg catcgataaa gcgggtacat ttagcattaa aaaatttaat cctgagacag
     7501 gtgagtttga acaattcagc aaaatggctc aaaaggaggt aacacaatga acatagaaac
     7561 tatcgtaaat caatttgaaa cacgagcagg cacgttacta aggtactaca ctggattatt
     7621 agaacatagt aaagtacaac cgtattgctt taagttatac aatgatccat ttgatatggt
     7681 atacgtgatg atgaacagca agctattcgg tcatgtatat attaaagatt gtaaagtaag
     7741 gcaatcattt gaattagcgt cacctaagca cactgagggg cttataagaa gcatagaggg
     7801 gcattatgta ggttatgaat tacatgacgg taaacatctt tctattagtg atatgatggc
     7861 tagtcatttg tttgaagatg agtattttat gtatggatta caaacatatg cagaatcaaa
     7921 taatagtgat gtgtttgagt acctagaaaa tggatttgat accgatacac ttgagggcat
     7981 tcaatcgagt aatactgatg tgatagcgaa tattgaaatg ttgtatcagt tagctacggg
     8041 aatcaatgaa ccagcaccag agttagttga gggattaaaa ttagtaactg agtttgtaca
     8101 agatgagaat gcgacacaag aggattacaa ggctttagaa cgtaagttaa ctgagttgaa
     8161 gtcatcttat tacagtttga ataagtaatt aaatatggag tcacacgtgg tgtgtggctc
     8221 ctaatgtaaa agtataaggt atagaagttt taaaatgtaa aggttgcaac aatagtgagt
     8281 taatagatag gtgtgcgaaa ttaaaaaaag tgtgaaatgt tgatattgag ctgttttatg
     8341 gctttgaaaa taataaggtt atataaaggt gttagctttt aaaatcggaa ggtatacagt
     8401 ctttgagaat tgaaaaaatg gcaagatttg tgcaaggtgt gcgaactttg ttaacgctaa
     8461 tacaagctaa agtttgtgtt tttggcatag gcctaaaagt taagtttgtt cgctgtttgt
     8521 tcgtataatt ttgacgaact taagttctat attaggttaa tgtgaaaagc ctaacgttaa
     8581 gtttaaaaca tgattttata agtgttatat atgataagct aaacaattga taaaacgcgc
     8641 tataaagcga acgtaagttt gttttagacc tgtaaaaatg gtataattta ggtatgaaat
     8701 aattaaaaga aagaggtgta gaaatgcaaa gtatcgcaga aaaagagacg tatcatttac
     8761 ccaccgaaca cctgcaagtt ttcaatgtga taaaaaatac gtccaataag tatattacta
     8821 aaactaaaat cttaaatcaa ttgggatatg aatataattc aagcaatgaa cgatggttac
     8881 gaagagtaat caattcatta gtatatgatt atggctatcc tatcgggtgc agttataaac
     8941 ctagtg
//
LOCUS       DAKSQE010000100         8853 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.100, whole genome shotgun sequence.
ACCESSION   DAKSQE010000100 DAKSQE010000000
VERSION     DAKSQE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8853
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.100"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            39..431
                     /locus_tag="O0V33_002100"
     CDS             39..431
                     /locus_tag="O0V33_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976721.1"
                     /translation="MQFKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFG
                     DTFLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLM
                     LTDFQKDNYNKYIQGQKQRVIFENGHFV"
     gene            complement(560..1936)
                     /locus_tag="O0V33_002101"
     CDS             complement(560..1936)
                     /locus_tag="O0V33_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCX8976722.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAT
                     NFGWQTNFIISIGIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSFICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTSLQPSQYIIAVVIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
     gene            2170..3162
                     /locus_tag="O0V33_002102"
     CDS             2170..3162
                     /locus_tag="O0V33_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCX8976723.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWTSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKADAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDVTGHSAEVSFKEGE
                     VVIKDIPIGVLTNHPDLDWHYSNLRQYINISPYPATAKLLEGVSIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNEIVALKLTDDLINRKDMMIFKPEKHITIRKLNDNQ"
     gene            3488..4438
                     /gene="lytM"
                     /locus_tag="O0V33_002103"
     CDS             3488..4438
                     /gene="lytM"
                     /locus_tag="O0V33_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HCX8976724.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHKHYFYNCYPKNANAN
                     GSGQTYVNQATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHNGN
                     GKVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR"
     gene            complement(4490..5149)
                     /locus_tag="O0V33_002104"
     CDS             complement(4490..5149)
                     /locus_tag="O0V33_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8976725.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFESNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENIKFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWAYTY
                     Y"
     gene            complement(5163..6083)
                     /locus_tag="O0V33_002105"
     CDS             complement(5163..6083)
                     /locus_tag="O0V33_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8976726.1"
                     /translation="MKPYIQLVVFKQWLQYILLVTTIVIALVLIGMGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAVEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHIPESKIKFVSLTKQAQHSISISLIFAVILFVSAVQVILHYRLNQQAALQRLS
                     QYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLSIFM"
     gene            complement(6080..7246)
                     /locus_tag="O0V33_002106"
     CDS             complement(6080..7246)
                     /locus_tag="O0V33_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8976727.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALLITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKQQKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLTTVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRALITWFYTMLWSIVGVVWIVFSIPNTFELYN
                     WPTLAIHLSYYVTFLILWLLIIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            complement(7314..8837)
                     /locus_tag="O0V33_002107"
     CDS             complement(7314..8837)
                     /locus_tag="O0V33_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976728.1"
                     /translation="MSKKLKIIIPIIIVLLLIGGIAWGVYAFFANTPKNTYLKSEQQT
                     AKMYKDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIK
                     MSYGHDPKKEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLST
                     YSKLTGEDEETAKENGITNQQLNLNTLFSNAQAQQSDYSKIAEKYSELIVDKLDDDNF
                     DKGKKEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGTTKD
                     FEKDIKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLE
                     DDKLKLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYSDKYEFGKKTEYDESKIKLD
                     NQEKVDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEP
                     INFILKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
BASE COUNT         2970 a         1402 c         1524 g         2957 t
ORIGIN      
        1 attaaggctt gttaaaactt aagagaggat gtttttaaat gcaattcaaa ttaaaagaag
       61 aagagattat tagtttttta gaattgaaat atccagaaaa agagttcgaa tatggtcgtt
      121 tgttagttgg acaacataaa cgcgatgatt tagatgttta ttactttggc gatacgtttt
      181 taatgtgcac gattatttca ttcaagacat ttgaaattaa agaaacagta gaattatcat
      241 atgatgctgt taatcgtatt gtgttaaaag atggatggtt atttagaaaa atgagaatag
      301 aaacaatgca aaaagtgtta aaatacggta catctaaatt aatgttaact gattttcaaa
      361 aggacaatta taataaatat attcaaggtc agaaacaacg cgtgatattt gaaaatggcc
      421 attttgtcta attgatagtg aataataatt agaataagcg aagaacaatg aaaaatgttc
      481 ttcgcttatt tttatagaat ttgatgtata tcattttaaa gtgataaaga tatttcgaaa
      541 cataattctt ctattatatt taagatttaa ctgtttttgg aatgatcatg tatgcagaca
      601 atgagccgag tatcattaat acaatgctga cgatgaatgt tacagttgca gctacacttg
      661 gtgcatagtt tagttgtaac atactgaaaa ctgtagtact tagtgctata ccaaaggcgc
      721 cacctaatgt accactcatt ttatataatc ctgtagctaa accaactttt tcatttggca
      781 tactgaaaat tgcaattgta aggccgggtg ttgcgactaa accatttcca attgcgcaaa
      841 tgacaaaccc aataacaaca gcaatgatat attgtgatgg ttgaagtgat gtcatgctaa
      901 taatagtgat gccgatgaca gggaacaacg gaccaatgat gagcatcaat ttgccaccga
      961 aacgtaatgt tgctttttca cctaaacgaa tcatcgcaac tgccacaatg gcatatggca
     1021 atgtaacaag tccagattgc gcagctgata aaccaaggtg tgtttgagca tatatgaaaa
     1081 agaccactgt tacgcctaga ccgctattta aaacaaagtt atttaaaaat gcaccaatga
     1141 acggacggtt gcgtaatact gagaaatcaa taaaaggtac ttcatgtcga cgttcgatga
     1201 tgatgaatat cagcgtagtg atgataaaaa tgctcagaca aatgaatgaa aatgtactaa
     1261 accaaccttg ttcgaatcct tgtgttaaca ataatgtaaa gctaccaatc ataacagcga
     1321 aaatcgacat acctttgtaa tcgaatggat gacggtggct atgttgactt actttttcag
     1381 gtgtgccttt tagaagtaat atggcaatga aagcaatgcc tatactaatg atgaaattcg
     1441 tttgccatcc gaaatttgtg gcaattaaac cgccgataac accagctagg ccaatgcccc
     1501 caacagtact aatcattaga taactaatcg ctcgtcttaa atgttctcct ttaaattgat
     1561 tatttaaaac gccaactgtt gaaggtaaca agatagctgc tgatagacct tgtaaaattc
     1621 taccgatgat gagcagtgca gtgatgtccg atataattaa tagaagagat gcaaacatac
     1681 tgattatgag acccatgtat gtcattctca gttgtcctat tttatcagca atatcacctg
     1741 cagctaccat gaagatacct gtggcgaagg aagttaaact aatagataaa tttaacacgg
     1801 caggagaggt ttgatacgtt tgaccaacga gaggtcctat attaataaat gattgtgcaa
     1861 acaaccaata tgttaatgca gacaacataa tcgcaataat aatattactg cgtggtgaag
     1921 attgtgtgtt attcacagat gtcactcctt gtaaaaatta aatatagaaa gtcatgagat
     1981 gaggtgaacg gatgtataga tgatcgttca ctcacacata agtcatgatt tccttacata
     2041 tcataccaca gttattgaga atgaatatca attaattgct tgtatgattg attttttata
     2101 atatgtttaa gtgaaacagc tttatttgaa agtgattaaa gtaaatgaag gtacagagga
     2161 gtgagaacaa tgtgcacagg attcacaata caaactttaa ataatcaagt acttcttgga
     2221 cgcacgatgg attatgatta tccattagat ggttcgccag cagtgacgcc tagaaattat
     2281 cgttggacat ctcgcactgg cacgacaggc caaacccaat atggctttat tggcacagga
     2341 acagatatgg aaggttttat ttatggtgat ggtgttaatg aacatggcgt tgccatttca
     2401 acacaatatt tccgaggtta tagttcatat ggatcaacac acaaagcgga cgcgatgaat
     2461 attacgcaaa atgaaattgt gacatggatt ttgggatata caacaagcat tgaagatatg
     2521 aaacaacaag catcccaaat acatgttgta gcggtatatt taaatgatat cggtgaagtt
     2581 ccgccattgc attatcatgt ttccgatgta actggacatt cagccgaagt ttcatttaaa
     2641 gagggtgaag tggttataaa agatatccct attggtgtct taacaaatca tccagactta
     2701 gattggcatt atagtaattt aagacaatat atcaatattt ctccttatcc agcaacagca
     2761 aagttattgg aaggtgtatc gattgaacct ttaggcaatg aagcaggtac atttggattg
     2821 ccaggtggat ttacttcaac tgagcgcttt gtgagaatgg catttatgaa agcaaacatt
     2881 gctcaaaaca atgataaaga aatggattta atgaatgcat tttatttatt agatgcggta
     2941 aatataccga ttggaattgt acgtccgcat gatgctgaca atcactatac gatgtatcag
     3001 accgtaataa atttaactac aagaacgtta tatattaagt attatggcag caatgaaata
     3061 gtagcattaa agctcacaga tgatctaatt aatagaaaag atatgatgat ttttaagcct
     3121 gagaagcata tcactattag aaagttgaat gacaatcaat agcgattgaa aatggaattt
     3181 ggttgatatg atttgatgga tgattactat catacattag cgttgaaccg cgaacatgga
     3241 cgaatgatta gttgttaatt ttgaaaattt ataaaagctt aagcgaacgc agtgagagat
     3301 gtcattttaa tatcaatgta tggctatttt gtaatgacaa tgtaatgagt ttagtaaaac
     3361 atttcgggaa tattaaatag ttggaaaatg agaattaaat cctttactca gttgtctaat
     3421 tcttttagta tgtgcagtac agtttaattg aaaactaact ttaactttaa tggaggatgt
     3481 tttatacatg aaaaaattaa cagcagcagc gattgcaacg atgggcttcg ctacatttac
     3541 aatggcgcat caagcagatg cagcagaaac gacaaacacc caacaagcac atacacaaat
     3601 gtcaacacaa tcacaagacg tatcttatgg tacttattat acaattgatt ctaatgggga
     3661 ttatcatcac acacctgatg gtaactggaa tcaagcaatg tttgataata aagaatatag
     3721 ctatacattc gtagatgcac aaggacataa gcattatttt tataactgtt atccaaaaaa
     3781 tgcaaatgcc aatggaagcg gtcaaacata tgtgaatcaa gcaacagcag gagataacaa
     3841 tgactacaca gcgagtcaaa gccaacagca tattaatcaa tatggttatc aatcaaatgt
     3901 aggtccagac gcgagctatt attcacatag taacaacaac caagcgtata acagtcataa
     3961 tggtaatgga aaggtcaatt accctaatgg cacatctaat caaaatggcg gatcagcaag
     4021 taaagcgaca gctagtggtc atgcgaaaga cgcaagctgg ttaacaagtc gtaaacaact
     4081 acaaccatat ggacaatatc acggtggtgg tgcgcattac ggtgtcgact atgcaatgcc
     4141 tgaaaattca ccagtttact cattaactga tggtacagta gtacaagcag gttggagtaa
     4201 ctatggtggc ggtaatcaag taacgattaa agaagcgaac agtaataact accaatggta
     4261 tatgcataat aatcgtttaa ctgtttcagc tggtgataaa gtcaaagctg gtgaccaaat
     4321 tgcatattca ggtagtacag gtaattcaac agcacctcac gtacacttcc aacgtatgtc
     4381 tggtggcatc ggtaatcaat atgcagtaga cccaacgtca tacttacaaa gtagataata
     4441 cagtaaatcc caagttacga tatcatacgc aacttgggat tttttcgttt taataatagg
     4501 tataagccca ttgcttgtcc tctaaaacat gttgataaaa aggatcttgg ccaattaact
     4561 tgatatcatc tgcaagtgct tcaacttcat ctaaatgatg ggtagttaat ataattaaac
     4621 atttagattt cataatgtta agtagttggt ggatgtcatg tctagatttt aaatcaatac
     4681 caactgtcgg ttcatctaaa ataagaattc gaggttgacc tagtaaacct actaatatat
     4741 taattttacg tttattccca ccggacaatg tagatacttt ggcagacgta tcatcaaagt
     4801 ttaattgctg taaatattcg ttgatagttg tatcgttaat tggattttta caaagtgatt
     4861 taaaaaattt aatgttttca gccactgtca tgtgctcaaa taacgcaatg tcttgtggca
     4921 cataaccgat gtgattttgt atttgtcttt gattccattt ttcgccgaaa tagttgatag
     4981 ttccatcatt agctttttca ataccagcaa tcatacgaag taatgttgat tttccagcgc
     5041 cattatcacc aagtaatacg gttaaacgat tactttcaaa ggacatggtt aaatgattga
     5101 aaatctgttt gttgcggtaa cgctttgaaa ggttattaat ttctatcatc agttacgctc
     5161 ctttacatga aaatgctcaa gtatacgata cccatagcaa gtgcatatat aaatgtcatg
     5221 aataatcgat ggcttattgt ttgaatatgg aataagataa agacgatacc tatctcataa
     5281 atcaatataa gtagcagtga ttttaagtaa aatattaagc tgagtggttg agacaaatat
     5341 agactaactg ccaatagtac caacaataac aaaatagtat gtgtcattac ataagtacta
     5401 tatagtttga aacggcttaa atgatattgt gataatcgtt gcaatgctgc ttgttggttt
     5461 aaacgataat gaagtattac ttgaacagcg ctaacaaata aaatcaccgc aaagattaag
     5521 ctaattgaaa tagagtgttg tgcttgttta gtaagcgaca caaatttgat tttagattca
     5581 ggtatatgtt tatgatagga cttgttgata gcatcgatgg attgatgctg tttcatatcc
     5641 tcaaggtgtt cataaataat gttaggaatt tgctgttcat ataatgagct actgacaatt
     5701 tctacagcaa taccacctat aaagtcatct ctaccatata actgtatcgt ttctttcaaa
     5761 cggttctctt ttaatttttg agagaaacct ttaggaattt gcatacttaa aatagcttcc
     5821 tttttagtaa catcatcttc aatatagctt tcatcttcat cgactttttt aatagttaca
     5881 tagtcagatt gtttaatttt attgacgaat gattttgatg cagtggtttg gtctaaatct
     5941 tgaatggtaa tcggtatttt gaagttgtca tgtgctacac ggtaacccat accaataagt
     6001 acgagtgcga tgacaatggt tgttacgagc aagatgtatt gtaaccattg cttgaacaca
     6061 acaagttgta tataaggctt cattgacgat acctccaaac caatacagct aaattaatta
     6121 tcaaaagtgc gatgaagcta agatagaaac tagggtgcag ttctaaaatg tagttgttta
     6181 aaataatttc taacaattga tttgttacaa ctgcgaacgg ttgaatattg aaaacgccat
     6241 ttgctatatg ttgtaaaaaa atcgtaggta ttgttaaacc agataacacc aggatgacaa
     6301 tagctaatat gactttacta atactattca acaagcctgt tgttaaaagt tcgatgatta
     6361 ataaccacag tattaaaaag gtaacataat agcttaaatg aatggctaac gttggccaat
     6421 tatataattc aaaggtattc ggaatactga acacaatcca aactacacca acgatactcc
     6481 ataacatagt ataaaaccat gttatcaacg cacgaatgat taataaacgc tctttagaaa
     6541 aatgaaacat tttcaatcgc gctttcaata cagtatcttg attcattttc aaaactgtaa
     6601 ataaagatag tgcaaagatg aataccgttg ttaaaaatcc tgtaattgca taataactgc
     6661 ccgtatcgta taaatgaatc ggttctaagt taaatgcacc tgaacggttt aatcctgtaa
     6721 tcagcaaatc agtcataaca ttgatactgt cagaatgtga tgctttcggt gctaggtctt
     6781 gaaaagctaa gatgccaccc attgaccgca taaggcgttg gtaaacagaa tctgttagct
     6841 gagatagcac gacacttttc atggattgtt gatcatatgt atatactgaa attggtagtt
     6901 caccttgttt ataaaatgcc ttggtcatac ctttatcaaa aacaaaatag ccttgaagtt
     6961 tctgctgttt tagcaaagta tgtgcttgct tatcatcata tgctttaatg ctcacatttt
     7021 tacctaggtt actcccttta ccaatagagt ttaagattaa tttcgtttca cttgattggt
     7081 ctttatctac gacacctata ttaaaatgat tgtcatcttc tgttacatgt tggattgtcg
     7141 ttaatgtaat aaggagtgcc gctaatataa atagtaaata gataatcaaa taccactttt
     7201 tcaataaaaa agagtggtag atgcgaaaca aatgtattgt tttcatttaa ccactcctcc
     7261 aatgataaga ttgaaaggca agatgacctt ccaatcttat ttttatattt tagttattta
     7321 gatgcctttt ttaaaattga ttcaaacatt ttgccgccat ttttttcgat ttctttttca
     7381 agtttttcac ggtcttttga agatagacta ttgaaatctt tcgcaccact atcatcaaaa
     7441 tcaatatctg ctttcaattt tgtgctagat tttaaaatga aattaattgg ttcttcagca
     7501 tatttgatgc cgatatttaa cgtagatttc tgagtgttat tttttacgtc agaatctata
     7561 ttattttcaa aagtgaattc attttcgtcg ctatatttat ctaacgcgac agtgatttta
     7621 cctttatctt gacgttttgt gccatctact ttttcttggt tatctaattt gatttttgat
     7681 tcatcatatt ctgtcttttt accaaattcg tatttatcac tgtatttatt gtctttttcc
     7741 ttagaagata cgcctttaat tgtatatttc gcttcagcat acgtgtattt atcttgatcg
     7801 aaatcaagtg cgtaatctag ttttaactta tcgtcttcta aagtattagt acctttgatt
     7861 ttagttttat tattttcttt gtctgtaata gtaatttctc gttttacaat cgtatgtttt
     7921 tcggtataaa ttttagattg aattttagca aattcatcct ttttagtttc tttgacatcg
     7981 tcaattgctt ttttaatgtc tttttcaaag tcttttgtag taccttgttc ttccattaat
     8041 tttttaaggt ctttatcctt tttagcttct tctaatacag ctaatgtaat ttttttagtg
     8101 tcagctctgc taagtgttaa cgtgacaggt ctaactttgt acttttcacc attaacctta
     8161 atttcttctt ttttaccttt atcaaaatta tcgtcatcta atttgtcgac aataagttcg
     8221 gaatattttt cggcaatttt gctgtagtca ctttgttgtg cttgagcatt actgaaaaga
     8281 gtatttaaat ttagttgttg gtttgtaata ccattttctt ttgctgtttc ttcatcttca
     8341 cctgtaagtt ttgaataagt tgataataaa tcagaattat taacactata tttcccttta
     8401 aataatggtg attcgaaata atgcttatct ttatctgcag ctaactggaa tttccctaat
     8461 gcagagtctg ctattgttgg ttcaagatta atcattgatt tctctttttt aggatcatgt
     8521 ccatatgaca ttttaatttt cgaagcatta acaacagatt taggaatacc aagcccttta
     8581 acaatttcat cagatgcatc tgcgcttaat tctaatgaag ataaaaatga attatctttc
     8641 atcttttctt ggaacttcac ttcgttttca aaacggtcat taaaataatc tttatacatt
     8701 tttgcagttt gttgttcact ttttaagtat gtatttttcg gtgtgtttgc aaaaaatgca
     8761 taaactcccc atgcgattcc acctattaat aataagacaa taataatagg aattataatt
     8821 tttaactttt tagacatttt gcttcctccc aat
//
LOCUS       DAKSQE010000101         8850 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.101, whole genome shotgun sequence.
ACCESSION   DAKSQE010000101 DAKSQE010000000
VERSION     DAKSQE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8850
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.101"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            30..143
                     /locus_tag="O0V33_002108"
     CDS             30..143
                     /locus_tag="O0V33_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976729.1"
                     /translation="MNGILIKCLNIGGQYYLQIRMLSNEDNAEKTISGNSI"
     gene            525..785
                     /locus_tag="O0V33_002109"
     CDS             525..785
                     /locus_tag="O0V33_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976730.1"
                     /translation="MIIMRGYNERYCSWTNTAIQCDYFNKVRQVKKDGKSDRLSNNIN
                     SNIIYRVFEKCITLYNVYSRCKRALILSEHSKKNIFNIKKAV"
     gene            795..1292
                     /locus_tag="O0V33_002110"
     CDS             795..1292
                     /locus_tag="O0V33_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCX8976731.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISANIEILL
                     QESSS"
     gene            complement(1400..1492)
                     /locus_tag="O0V33_002111"
     CDS             complement(1400..1492)
                     /locus_tag="O0V33_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976732.1"
                     /translation="MSVINAYSHASLLFFIYLLFIMYQISLSVK"
     gene            1683..3563
                     /locus_tag="O0V33_002112"
     CDS             1683..3563
                     /locus_tag="O0V33_002112"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HCX8976733.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQETSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDALLENLGAERICKRVDCDIDYEED
                     AEKWMADIINIIDTTSEGIQSESVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGWDPQSPVPINDHGDT
                     VPIAEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAHEYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESERF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAETILRQMKQRQRYQRD
                     VY"
     gene            3629..4237
                     /locus_tag="O0V33_002113"
     CDS             3629..4237
                     /locus_tag="O0V33_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HCX8976734.1"
                     /translation="MSMPLMIDLTNKNVVIVGGGTVASRRAQTLSQYVEHMTVISPTI
                     TEKLQNMVDKGVVIWKGKEFEPSDIVDAYLVIAATNEPRVNEAVKQALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKLLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVRQ"
     gene            4310..4423
                     /locus_tag="O0V33_002114"
     CDS             4310..4423
                     /locus_tag="O0V33_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976735.1"
                     /translation="MGGFVNSLIMKDLSDNDMRFKYEYYNREKIRRTNLIK"
     gene            4726..6096
                     /locus_tag="O0V33_002115"
     CDS             4726..6096
                     /locus_tag="O0V33_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HCX8976736.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVI
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWFGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKPWIKWVNTALTLAVILAMLINIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
     gene            6343..8193
                     /gene="nrdD"
                     /locus_tag="O0V33_002116"
     CDS             6343..8193
                     /gene="nrdD"
                     /locus_tag="O0V33_002116"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCX8976737.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNHLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPNWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYTSG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            8190..8726
                     /gene="nrdG"
                     /locus_tag="O0V33_002117"
     CDS             8190..8726
                     /gene="nrdG"
                     /locus_tag="O0V33_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCX8976738.1"
                     /translation="MTLLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDKILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNTKTIWVWTGFLYEYLANDCTERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
BASE COUNT         3183 a         1235 c         1707 g         2725 t
ORIGIN      
        1 caatatgaaa aataatatga ttaaaaataa tgaatggcat acttataaag tgtctaaata
       61 ttggtggtca atattacttg caaatacgaa tgttaagtaa tgaagacaat gctgaaaaaa
      121 caataagtgg aaactctata tagaatgttc gtttttattt ttaataattg aataatcatt
      181 attgatgatt taatatgtat tcggtcgcac acgatacaaa gtgacttgtt ttaatgatat
      241 gcaataatat gacgtttatc gtcgcgtgta acaatgtatg agtgtgtcag tttaatttta
      301 aaagggttat aaggatattt aataagataa aggagggatt aataaacaat cacatgtgaa
      361 gtttttaagg attaaatgag ttgggcagct ctttaatcca tagttataac gaattttaaa
      421 ttgttagatt taacgtatgg tatcagtatg tatgagttta acttaagtat taatctatag
      481 tttaaagagc ggtctaattg tctttttaaa aatagaaaat aaggatgatt ataatgaggg
      541 gttataacga aagatattgt agctggacta atacagctat acaatgtgat tattttaata
      601 aagtaagaca ggtgaaaaaa gacggtaaat cggataggtt gagcaacaac ataaactcga
      661 acattattta tcgtgtgttt gaaaaatgta tcacgctata taatgtgtat tcgcggtgta
      721 aacgtgcact tattttatca gaacattcta aaaagaacat attcaatata aaaaaggctg
      781 tgtaggggtg tcttatgaat aatagaaatg tgtatgatat cgaagtaagt gattataaag
      841 gcttaactta taaattagaa gcatttagag gtaaagtgat tttagttgtt aatactgcaa
      901 cagaatgtat atatagcgaa caattgaaaa aactagagac tttgtttcaa aaatataagg
      961 atcgtgggtt tgtagtgttg agttttccga ataataattt tgacaatcga caaccaggtt
     1021 ctaatgaaga aatcttgaaa attagtcgtg agaaatttgg tgttacattt ccagtgctag
     1081 ctaaaatatc tgtgaacggg aacgatgaac atccgttatt tacgcattta aagaatgaac
     1141 aaccaggaat ctttgggtcc ccaataaaat ggaatttcac aaaatttata atcgatcgac
     1201 aaggcaatgt tgtaaatcga ttcttgccaa tggaagatcc attggatata tcggcaaata
     1261 tagaaatatt attgcaagaa tcttcatctt aaatttaaaa ttgagcgctt agtatgcaaa
     1321 taacacaaga gcctcttaat gatgtactaa tagtatgtca ttaggaggca ttttatattt
     1381 agaaaatcag taatttgacc tattttactg aaaggctgat ttggtacata ataaatagta
     1441 aataaataaa aaataacaac gatgcatgac tgtatgcatt aataactgac acgacataat
     1501 caaagttttg tctgaattac tgtgtcattc aaaccacatg aaggttgtaa atatttgtaa
     1561 gtataactat cagacacaac tcaagtttgt tttaatttat accatacata ttaaaaaatt
     1621 gactgaacta tcgatgaaaa atgagtcaat catctatttc aagtttattg aggtgaaaat
     1681 atttgaaatt aaatacatct aatagtccat ttacagaaaa acaagtcaca gagattaaca
     1741 atctgctgca aacattaaca gagagccaac aacagtggct tagtggtttt ttactagcta
     1801 atagtaacga tacgacaagt gatagtaatc aacaacaatt agagacagaa gtgtggcaac
     1861 aatcacaaat atcagaagaa caagaaactt caacaacgta tatgttacaa aataaagagc
     1921 cacatatcga agctaatcag cggcatgtca cagtgctata tggttctgaa tcaggtaatg
     1981 ccatgcgttt agctgaaatt ttttcagaac gattaagtga tattggacat caagttgttt
     2041 tgatgtcaat ggatgaatat gatacgacaa acatcgcaca gttagaagat ttatttatta
     2101 ttacgtctac tcatggtgaa ggagaaccgc ctgataatgc atgggatttc tttgaatttt
     2161 tagaagacga taacgcacct aatttaaatc atgtgagata ttcagtacta gctttaggtg
     2221 atcaaacata tgaatttttc tgtcaagccg gtaaagatgt agatgcttta ctagaaaatc
     2281 taggcgctga gcgtatatgt aagcgtgtag attgtgatat tgattatgaa gaagacgcag
     2341 aaaagtggat ggcagacatc attaatatta ttgataccac atcagaaggt attcaaagtg
     2401 aatcggtgat aagtgaatca attaagtctg ccaaagaaaa gaaatattct aaatcaaatc
     2461 cataccaagc agaagtatta gcgaatatca atttaaatgg taccgattca aataaagaaa
     2521 cacgacatat agaattttta cttgatgatt ttagtgaatc atatgaacca ggagattgta
     2581 tagtagcatt accgcaaaac gaccctgaat tggttgaaaa actaatatcc atgttaggtt
     2641 gggatccgca atctccggtg ccaattaatg atcatggtga tacagttcct attgctgaag
     2701 cactaacatc acattttgaa tttactaaat taacattgcc attattgaaa aatgcagata
     2761 tctattttga caatgaagaa ttatctgaac gtattcaaga tgagtcatgg gcgcatgaat
     2821 atgttataaa tcgggacttt atagatttaa taacagattt tccaactata gaattacaac
     2881 ctgagaatat gtatcaaatc cttagaaaat taccaccaag agagtattcg atttctagta
     2941 gttttatggc aacgccagat gaagtgcata ttaccgttgg tacggttcgt tatcaagcac
     3001 atggacgtga gagaaaaggt gtatgctcgg ttcattttgc tgagcgtatt aaaccaggcg
     3061 atatagtacc aatttatttg aagaaaaatc cgaacttcaa atttccgatg aagcaagata
     3121 taccggtaat tatgattggt ccaggtactg gtattgctcc ttttagagca tatttacaag
     3181 aacgtgaaga acttggtatg actggaaaaa catggttgtt ctttggtgat caacaccgta
     3241 gttctgactt tttatatgaa gaagaaatag aagaatggct tgaaaatgga aacttaacac
     3301 gcgtagattt agcattttca agagaccaag aacacaaaga atatgtacag catcgtataa
     3361 tggaagaaag tgaacgtttc aatgaatgga ttgagcaggg cgcagcaatc tatatttgtg
     3421 gcgatgaaaa atgtatggcg aaagatgtcc atcaagccat taaagatgta ttggtaaaag
     3481 aacgtcatat ttctcaagaa gaagctgaga cgatattgcg tcaaatgaaa cagcgacaac
     3541 gttatcaacg cgatgtttat taatgcttca atgtgttaaa tgttttaagg tgaaatgacg
     3601 taaaaagata taaaggatgt agcgcaatat gagtatgcca ttgatgatag atttaacaaa
     3661 taagaacgtg gttatagtag gtgggggtac agttgcaagt cgtcgggcac aaacattaag
     3721 tcaatacgtt gaacatatga cggtcatcag tccgacaatc actgaaaaac ttcaaaatat
     3781 ggtagataaa ggtgtcgtca tatggaaggg aaaagagttt gaaccaagcg atattgtaga
     3841 cgcgtatcta gttattgcag caaccaatga gccacgtgtc aatgaagcgg taaaacaagc
     3901 cttacctgag catgcccttt ttaataatgt tggagatgca tcaaatggca atgttgtatt
     3961 tccaagtgca ctacaccgag acaagctaac tattagtgta tctacagatg gcgcaagtcc
     4021 taagttgaca aaatcgatta tggcagagct tgaggcgtta tatccaccat catatagttc
     4081 gtatatcgac tttttatata cttgccgaca gaaaataaag ttactagata taacatataa
     4141 cgaaaagcaa cagttactgt cacaaattgt gtcacaagaa tatttaaatc atgacaaaca
     4201 agctcaattt ttagcgtggt tggatgtaag acaataatag cgggccgtct aaccgtctaa
     4261 ggtaagtctt cttattttaa ctttaacgct taatcattga aattaagaca tgggtggctt
     4321 tgtgaatagt ctaataatga aggatttaag cgataatgat atgcgtttta aatatgaata
     4381 ttacaataga gaaaaaatac gtagaacaaa cttaataaaa tagatggata aattgaaatc
     4441 tggttgaagt cgtgactatc atagcgacct ttagccagat tttttgtgca atagaaagca
     4501 ataataaaaa tgataaatca aaatgaaata caggacagga tatacaagga ttagtcatgc
     4561 catgttatca agtaggaaaa tcaaacttca ctattgatag ttacacaaaa aagaaatttt
     4621 tgataaaatg agttaactta aatataaaaa attatattaa ttattatatt taagttaaag
     4681 agggggatta tgtaaattgt attaaaagtg gagggagaaa ataatatgaa tagtgataat
     4741 atgtggttaa cagtaatggg gctcattatt attatttcaa ttgtaggttt actcattgcc
     4801 aaaaagataa atccagttgt aggtatgaca atcatacctt gcttaggggc aatgatttta
     4861 ggatatagtg tgacagattt ggttggattt tttgctaaag ggttagatca agtcatcaac
     4921 gttgttatta tgtttatctt tgccattatt ttctttggca tcatgaacga tagtggttta
     4981 ttcaagccgc ttgtcaaacg cttaatatta atgacacgag gcaatgtcat tattgtctgt
     5041 gcaatgacag ctttaattgg cacaatagcc caattagatg gtgccggtgc ggtaacattt
     5101 ttgctttcta ttcctgcctt attaccttta tataaagcgt taaatatgaa taaatattta
     5161 ttgattctac tattagcatt aagcgcggcg attatgaaca tggtaccttg gggaggtccg
     5221 atggcgcggg tagctgctgt tttaaaagct aaaagtgtca atgaattatg gtttggatta
     5281 atacctattc aaataatagg tttcattctt gttatgttat ttgcggttta tcttggattt
     5341 aaagaacaaa agcgtattaa aaaagcaata gagagaaatg aattaccgca aacacaagat
     5401 atagatgtac ataaattagt tgaagtatat gaacgagatc aagatgtaag gtttcctgta
     5461 aaaggacgtg cacgaacaaa accatggata aaatgggtga atacagcttt aactttagct
     5521 gttattctag cgatgttaat aaatattgcg ccacctgaat ttgcattcat gataggtgtc
     5581 tcattggcgc ttgttattaa ttttaaatca gtggatgaac aaatggagcg attaagagcg
     5641 catgcgccga atgcattaat gatggctgca gtgattattg cagcaggtat gtttttaggt
     5701 gtactaaatg aaaccggtat gcttaaagcg attgcgacga atttaatcaa agtgattcct
     5761 gcagaagtag gaccatactt gcatattatt gtaggtttac ttggcgtacc attagattta
     5821 ttaactagca cagacgctta ttattttgct gtgttaccga tcgtcgaaca aacagcaggg
     5881 caatttggtg taccatctgt atcaactgct tattcaatgg tcatagggaa tattataggt
     5941 acatttgtca gtccgttttc accagcctta tggttggcaa ttggtttagc agaggcaaac
     6001 atgggcacgt atattaagta tgcattcttt tggatttggg gattcgccat cgttatgtta
     6061 gtaattgcaa tgttgatggg aattgtgacg atttaagtat gaaaaaatag aaactatgct
     6121 cccgttacaa aatgaaataa tagttgcata aagatgtcga aatgacggaa cgattcgaga
     6181 acaattttaa aaattaatga aataattgtg tagaaatatg aatttcacta aatgttaata
     6241 actttgtgac gttttagtta acagactaat aaaaatttga aaatactata tatagtggta
     6301 taacgtaatg agtagacaca atatatagga agaaggggta aaatgaatca aatcgaagaa
     6361 gcattaacgg gtttgatttc taaagatcct gctattgtta acgaaaatgc caacaaagat
     6421 agtgatacat tttcaacaat gagagattta acagcaggta tcgtttctaa atcttacgca
     6481 ttaaatcatt tattaccaaa gcatgttgca gatgcacatc aaagagggga catacatttt
     6541 cacgacttag attatcatcc attccaacca ttaacaaact gttgtttaat agatgctaaa
     6601 aatatgctac ataatggatt tgaaataggc aacgcgaatg taacttcacc aaaatcaata
     6661 caaactgcat cagcgcagct tgtacaaatt atagccaatg tttctagcag tcaatatggt
     6721 ggctgtacgg ttgaccgcgt tgacgaatta cttagtacat atgcacgaca taatgaagaa
     6781 caacatagga atatcgcaaa gcaatttgtc aaagaatctg aaattgatcg ttatgttgat
     6841 caacaagtca ctaaagacat caatgatgcg attgaaagtt tagaatatga aattaatacc
     6901 ttatatacat ctaatggaca gacacctttt gtaacattag gattcggctt aggtacagat
     6961 catttaagtc gcaaaattca acaagctatc ttaaatactc gtatcaaagg cttaggaaaa
     7021 gaccgcacga cagcgatttt cccgaaactt gtattttcaa ttaaaaaagg aaccaacttt
     7081 agtccgcaag atccgaacta tgacattaaa caattagcat taaagtgttc aacgaaacgt
     7141 atgtatccag atattttaaa ttatgacaaa ctcgtagaaa tattaggtga tttcaaagcg
     7201 ccaatgggtt gtcgttcatt tttaccaagt tggaaagatg cggaaggtca ttttgaaaat
     7261 aatggtcgtt gtaatcttgg tgttgttaca cttaatttac ctagaatggc attagaatct
     7321 gccggtaata tgacgaaatt ctgggaaatc ttttatgaac gtatcgatgt gttacatgat
     7381 gcattacttt atcgtataaa tcgtttgaaa gatgctgtac cgaataacgc accaatttta
     7441 tataaaagtg gcgcttttaa ctataaatta aaagaaacag atgatgttgc tgagttattt
     7501 aaaaataaac gtgcaacgat ttcaatgggc tatatagggt tgtatgaaac agctactgtt
     7561 ttctatggtc caaactggga aacatctcaa gaagcaaaag catttacgct tgaaattctt
     7621 aaagaaatga aacgttatca aacgaaatgg acagaattat atgacatttg gttcagtatt
     7681 tacagtacgc cgagtgaatc gctaacggat cgtttttgtc gtttagacca agagagattt
     7741 ggagatatta aagacattac agataaagga tattaccaaa actctttcca ttatgatgta
     7801 cgtaaagatg ttacaccttt tgaaaagtta gattttgaaa aagattatcc ttattatacg
     7861 agtggtggtt tcattcacta ttgtgagtat ccgaaactgc aacacaattt gaaagcacta
     7921 gaagcagtat gggactactc ttatgacaaa gttggttact taggtacaaa tattccgatt
     7981 gatcattgtt atgaatgtga ttacgatgga gattttgaag ctactgaaaa aggatttaaa
     8041 tgcccgaact gtggcaatga taatcctaaa acagttgatg tcgttaaacg aacatgtggt
     8101 tacttaggca atccagttca acgtccagta attaaaggcc gtcataaaga aatttgcgca
     8161 cgagtaaaac atatgaaagc gcctaaagaa tgacactttt agacattaaa caagggcaag
     8221 gttatattgc taaaatagaa tcaaatagct ttgttgacgg tgaaggagta agatgcagtg
     8281 tttatgtatc aggatgtcca tttaattgtg ttggatgtta caacaaagcc tcacaaaagt
     8341 tcagatatgg cgagaaatac actgataaaa tattagcaga aatattagat gattgcgatc
     8401 atgattatat atctgggcta agtctattag gtggcgaacc attttgtaat ttggatatta
     8461 cattaaatct tgtcaaagca tttcgagcac gttttggaaa tacaaagaca atttgggtat
     8521 ggactggatt tttatatgaa tatttagcaa atgattgtac agaacgtcga gagttattat
     8581 catacattga cgttttagta gatgggctat ttatacaaca cttattcaaa cctgatttac
     8641 catataaagg ttctttaaat caacgcatta tagatgtaca acaatcactc tcgcatgcgc
     8701 gtatgattga atatattgtt agttgaatat gtattagaag tcaaggtaac atccgttgcc
     8761 ttggcttctt tttaggttag gcacataatt gaaagttgat aaaagcaatt ctttagaaaa
     8821 acatattgat agaatatgac ctaacaatca
//
LOCUS       DAKSQE010000102         8788 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.102, whole genome shotgun sequence.
ACCESSION   DAKSQE010000102 DAKSQE010000000
VERSION     DAKSQE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8788
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.102"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            227..913
                     /gene="mtnN"
                     /locus_tag="O0V33_002118"
     CDS             227..913
                     /gene="mtnN"
                     /locus_tag="O0V33_002118"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCX8976739.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDIIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQVPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            933..1460
                     /locus_tag="O0V33_002119"
     CDS             933..1460
                     /locus_tag="O0V33_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCX8976740.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            1461..2561
                     /gene="yqeH"
                     /locus_tag="O0V33_002120"
     CDS             1461..2561
                     /gene="yqeH"
                     /locus_tag="O0V33_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCX8976741.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFGDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYSLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLI
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNLQNFDLNEVKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            2575..3381
                     /gene="aroE"
                     /locus_tag="O0V33_002121"
     CDS             2575..3381
                     /gene="aroE"
                     /locus_tag="O0V33_002121"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCX8976742.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELDGFNITIPHKERIIPYLDHVDEQAINAGAVNTVLIKDGKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            3385..3675
                     /gene="yhbY"
                     /locus_tag="O0V33_002122"
     CDS             3385..3675
                     /gene="yhbY"
                     /locus_tag="O0V33_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCX8976743.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATHSELVQVIGSMIVIYRESKENKEIELP"
     gene            3678..4247
                     /gene="nadD"
                     /locus_tag="O0V33_002123"
     CDS             3678..4247
                     /gene="nadD"
                     /locus_tag="O0V33_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HCX8976744.1"
                     /translation="MKRIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHHDFIDVQHRLTMIQMVIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENGMIAIQIPRVDISSTMIR
                     QRVSKGKSIQVLVPKSVENYIKGEGLYEH"
     gene            4237..4821
                     /gene="yqeK"
                     /locus_tag="O0V33_002124"
     CDS             4237..4821
                     /gene="yqeK"
                     /locus_tag="O0V33_002124"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCX8976745.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            4822..5175
                     /gene="rsfS"
                     /locus_tag="O0V33_002125"
     CDS             4822..5175
                     /gene="rsfS"
                     /locus_tag="O0V33_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCX8976746.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYGQVAY"
     gene            5178..5894
                     /locus_tag="O0V33_002126"
     CDS             5178..5894
                     /locus_tag="O0V33_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCX8976747.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYRHLADSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLTQVGFKHVETFT
                     DFNIDEHNEDAERLFFIAKK"
     gene            5934..6620
                     /locus_tag="O0V33_002127"
     CDS             5934..6620
                     /locus_tag="O0V33_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HCX8976748.1"
                     /translation="MVLLYQFLLRYKDFLAQWKLYIISAVVLIMVLIGFIFWRQDDYT
                     SRDFENKDTVLKQSTSENSSLSKLEDVQVKDGDNSKNKGPVYVDIKGAVKHPNVYKMT
                     SKDRVVDLLDKAQLLDDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQFGANSVHVKN
                     GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKT
                     FDKLNSYFTT"
     gene            6712..7173
                     /locus_tag="O0V33_002128"
     CDS             6712..7173
                     /locus_tag="O0V33_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCX8976749.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAEYLTKG"
     gene            7178..>8788
                     /locus_tag="O0V33_002129"
     CDS             7178..>8788
                     /locus_tag="O0V33_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HCX8976750.1"
                     /translation="MLYVALSMIVGVLWNSSKVLSTFLFILLLYIAYRKNKIVYAPIS
                     LFLIIFSSWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTNKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEINEQFKERVKEIGIYHLLAVSGSHIAAI
                     VFLIYQPLKRLNLPLFVIKGITIIVLALFAQYTNYAPSAVRAIIMTTLVLLITNQIKI
                     KGIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFI
                     AQLASFIVAIPNFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYL
                     VDLSFNFHDWLLDLFTRIKQSHFSVPKFNDWIFIIFIISVYYIFWLLAKRKYILVTFW
                     TIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQPSYS
                     ISKYHILPTLNERGINELEYLILTHPHND"
BASE COUNT         3165 a         1118 c         1630 g         2875 t
ORIGIN      
        1 taattgatat gattagtttt tgaaaaatac acaaattaga agcatctatt tcactgttat
       61 gagttcataa ataattattt atgataccaa ggtattgtga aagcttttta taaaaataaa
      121 gcaaaagttt tatacagtaa tgaaaatcat gatacttgtc gttgtagtga ttaaaatgtg
      181 ataaaataac aaatgctagg attttttaaa agaggtgaat caaaggatga ttggtataat
      241 tggtgccatg gaagaagaag taacaatatt aaaaaataaa ttaacacaat taagcgaaat
      301 ttcagttgca catgttaaat tttatactgg cattttaaaa gatagagaag tagtgattac
      361 ccaaagcggc attggaaaag ttaatgctgc aatttctacg acattattaa ttaataagtt
      421 taaaccggac atcattatta atacaggttc tgctggagct ttagatgaaa gtttaaatgt
      481 aggtgacgtt cttataagtg atgatgtaaa atatcatgat gcggatgcaa cagcatttgg
      541 ttatgaatat ggacaagtac cacagatgcc ggtagcattt caatcaagta aacctttaat
      601 agaaaaagta tctcaagttg tacaacaaca acaattaaca gctaaagtag gcttaattgt
      661 aagtggtgat agctttatcg gtagtgttga acaacgccaa aaaattaaaa aagcatttcc
      721 aaatgcgatg gcggttgaaa tggaagcaac tgcaattgca caaacatgtt atcaatttaa
      781 tgtaccattt gttgtagttc gtgcagtttc agacttagca aatggagaag cggaaatgag
      841 tttcgaagca tttttagaaa aagcagcggt atcatcaagt caaactgttg aagcattagt
      901 gtctcaatta taaaaaggta aaggtgagtt aaatgggttt agttcgcaag ttttttatgc
      961 cgaattcata tgttcaatca atatttcaaa ttgatttaga caagttagtg gacaaaggcg
     1021 ttaaaggtat tattacagat ttagataata cgctagtagg ttgggatgtt aaagaaccta
     1081 cagaacgtgt taaagcatgg tttaaggaag ctaatgaaaa aggaatcact attacaattg
     1141 tgtctaataa taatgagtct cgtgttgcta gttttagtca gcatttagac atcgatttta
     1201 tttttaaagc gagaaagcca atgggaaaag cgtttgataa agcaataact aagatgaata
     1261 taagacctga tcaaactgtt gttataggtg accaaatgct tactgatgta tttggtggta
     1321 accgtcgagg tttatataca attatggtcg ttccagttaa acgaacggat ggctttatta
     1381 ctaagtttaa tagattaatt gaaagacgat tattacgtca tttcagtaaa aaaggttata
     1441 tcacatggga ggaaaattga ttgtctgaca ttttaaaatg tatcggttgt ggtgcgccac
     1501 ttcaatctga agataaaaat aaacctgggt ttgtaccaga gcataatatg tttggtgatg
     1561 acgtgatttg cagacgttgt ttccgcttga aaaattataa cgaagttcaa gatgtaggat
     1621 tagaaagtga agacttttta aaattattat caggacttgc ggataaaaag ggtattgtcg
     1681 tcaatgttgt ggatgtattt gactttgaag gatcatttat taatgcagtt aaacgtattg
     1741 tcggaaacaa aaaaatcatt ttagcagcaa ataaattgga tttattgccg aagcaaatta
     1801 ataaacgtcg agttaaagaa tggctaaaac gaacagcaag aaaatatagt ttggaagctg
     1861 acgatgtcgt attaatttca gctgaaaaag gctggggcat agacgactta ttatcatcaa
     1921 ttgcgaatat tcgagaaaat gaagatgtgt atattgtagg gacaacgaat gttgggaaat
     1981 ctacattgat taataaactg attgaagcta gtgttggtga aaaagatgta gtaacaactt
     2041 caagattccc tggaacaact ttagatatga tagatattcc attagatgaa acatcattta
     2101 tgtatgatac accaggtatt attcaagatc atcaaatgac gcatttagtt agtgaaaaag
     2161 aattgaaaat tattatgcct aagaaagaaa taaaacaacg cgtatatcaa ttaaacgagg
     2221 cgcaaacatt attcttcggc ggtctagcgc gcatagatta tgtatcaggt ggtaaacgtc
     2281 cgttaatttg tttcttttct aatgacttga atatacatcg tactaaaacg gagaaggcca
     2341 atgatttatg gcgtaatcaa cttggcgatt tattaacgcc acctggaaat ctacaaaatt
     2401 ttgatcttaa tgaggtaaag gctgttagac ttgaaacagg caaagagaaa cgcgatgtta
     2461 tgatctctgg tctaggcttt ataactatag gaccaggagc taaagtaatc gttcgtgttc
     2521 ctaaaaatgt tgatgttgta ttaagaaatt ctattttata aggtgattta aaaaatgaaa
     2581 tttgcagtta tcggaaatcc tatttcacat tccttgtcgc ccgttatgca tagagcaaat
     2641 tttaattctt taggattaga tgatacttat gaagctttaa atattccaat tgaagatttt
     2701 catttaatta aagaaattat ttcaaaaaaa gaattagatg gctttaatat cacaattcct
     2761 cataaagagc gtatcatacc gtatttagat catgttgatg aacaagcgat taatgcaggt
     2821 gcagttaaca ctgttttgat aaaagatggc aagtggatag ggtataatac agatggtatt
     2881 ggttatgtta aaggattgca cagcgtttat ccagatttag aaaatgcata cattttaatt
     2941 ttgggagcag gtggtgcaag taaaggtatt gcttatgaat tagcaaaatt tgtaaagccc
     3001 aaattaactg ttgcgaatag aacgatggct cgttttgaat cttggaattt aaatataaac
     3061 caaatttcat tggcagatgc tgaaaagtat ttagctgaat tcgatattgt tattaataca
     3121 acaccagcgg gtatggctgg aaataacgaa agtattatta atttaaagca tctttctccc
     3181 aatactttaa tgagtgatat tgtttatata ccatataaaa cacctatttt agaggaagca
     3241 gagcgcaagg gaaaccatat ttataatggc ttagatatgt ttgtttacca aggtgcggaa
     3301 agctttaaaa tttggactaa taaagatgct gatattaatt ctatgaaaac agcagtttta
     3361 caacaattaa aaggagaata acatatgctt actggcaaac aaaaaagata cttaagaagt
     3421 ttagcacaca atattgatcc gatttttcaa attggaaaag gcggtatcaa cgaaaacatg
     3481 attaaacaaa tagatgatac gttagaaaac agagaattga ttaaagtaca tgtactacaa
     3541 aataactttg atgataaaaa agaattagct gaaacattaa gcgaagctac gcatagtgaa
     3601 ttagtgcaag tgattggatc tatgatcgtg atttatagag aatctaaaga aaataaagaa
     3661 attgaattgc cataataatg aaaaggatag tactttacgg cggtcagttt aaccctatcc
     3721 atactgcaca tatgatagta gctagcgaag tatttcatga attacagcca gatgaatttt
     3781 attttttacc tagttttatg tctccattga aaaagcacca tgattttata gacgttcagc
     3841 acagattaac aatgatacag atggttatcg acgagcttgg ttttggagat atttgcgacg
     3901 atgaaattaa acgtggtggt caaagttata cctatgacac gatcaaggca ttcaaggagc
     3961 aacacaaaga cagtgagttg tactttgtta ttgggacgga tcagtataac caactagaga
     4021 aatggtatca aattgaatac ttaaaagaaa tggttacttt tgtagttgta aatcgagaca
     4081 aaaatagtca aaatgttgaa aatggtatga ttgcaattca gatacctagg gtagatataa
     4141 gttcgacaat gattcgacaa agagttagta aaggtaaatc tatccaagtt cttgttccta
     4201 aatccgttga aaactatatt aagggggaag gattatatga acattgaaaa agcaaaacgg
     4261 cttgcaaaag agaaactgcc agagaaacgt tataatcatt ctttaagagt tgcagaaaca
     4321 gcgattaaat tagcagaaat ttatgatgga gacactagta aagtagaatt agcaggtgta
     4381 ttacatgatt tctgtaaata tgatgattta ggtaaaatgt atcaaattgt tcgacaatac
     4441 gaattaggta atgatctact gagttatggt agtgaaatat tgcatggccc agtgtgtgca
     4501 gcaatcatgg aacatgaata tggtatcaat gatgaggaag tattaatggc tatcaaatac
     4561 catactacgg gacgtcaaca aatgacaaaa actgaaaaac tgatttttat tgcagattac
     4621 atcgaacctg gtagaacaat cccaggagtt gatgatattc gagatatggc atacaatcaa
     4681 ggtagtttag ataagacaat ttatgaaatt tctaaacgca cagtactatt tttaatacag
     4741 aaagatatta cggtatataa taagacaatt gactgtttaa attattataa ctatagtgac
     4801 gaaagaataa aggatgatta aatgaattca caagaattat tagcaattgc tgtggatgca
     4861 attgacaata aaaaaggcga agatacgatt tctttagaaa tgaaaggtat cagcgatatg
     4921 acagattatt ttgttgtaac gcacggaaat aatgaacgac aagttcaagc gattgctaga
     4981 gcggtgaaag aagtagccaa tgaacaaaat atagaagtaa aacgtatgga aggttacaat
     5041 gaagcgcgtt ggatattaat tgacttagct gatgttgtgg tacatgtttt ccataaagac
     5101 gaacgaaatt attataatat tgaaaagtta tatcaagatg caccattaga atcatatggt
     5161 caggttgcgt attaattatg tcgcaatatg cagaaatgag cctagtgtac gatcaattga
     5221 ctcaagatca accatatgaa aaatggtttg aaattgtaaa aaatcactgc aaagatgaat
     5281 caaatatttt agatattgga tgcggtactg gtagtttaac agttcaatta gaagctttag
     5341 gtaatgttac tggtatggat ttaagtgttg atatgttgac tgttgcagct caaaaatcag
     5401 caaatgtaaa ttggcttgaa ggagatatga catcgtttga tttgcaacaa caatttgata
     5461 tcatcactat tttttgtgac tctctaaatt atttgcaaga tgaaactgct gttattgaaa
     5521 cattcatcaa tgtttatcgt catctggctg attcaggtgt atttattttt gatgtacata
     5581 ctgtttataa aatgatgact ttgtttaata atcaaagtta tattgatgat aaaggggaca
     5641 tttttttagc atgggatgct gtgcaaggag atttaccttt aagtgtttat catgatatga
     5701 catttttcat tcgacatgaa gatgaaacat attcaagatt tgacgagtct cattttcaga
     5761 gaacatttga tgaaaagaca tatttgtctt ggttaactca agttggtttt aaacatgttg
     5821 aaacatttac tgattttaat atagatgaac ataatgaaga tgcagaaaga ttgtttttca
     5881 ttgcgaaaaa ataaaagtaa aaccactcgc tcagacgtgt ataaagttga ggagtggttt
     5941 tattgtatca atttttatta cgctataaag attttttagc tcaatggaag ttatatatta
     6001 taagtgctgt tgttttaatt atggtattaa ttggttttat attctggaga caagatgatt
     6061 atacttcaag agattttgaa aataaagata ctgttctgaa acaaagcact agtgaaaata
     6121 gtagtttgtc caaattagaa gatgtccagg tcaaagatgg agataattcc aaaaataaag
     6181 gtcctgtata tgtcgatata aaaggtgctg ttaaacatcc taatgtttat aaaatgacat
     6241 ctaaggatag agtagttgat ttacttgata aagcacaatt attagatgat gcagatgtaa
     6301 gtcaaattaa tttgtctgaa aaattaacag atcaaaaaat gattttcatt cctcataaag
     6361 gacaaaagaa tgttgaacca caatttggag caaacagtgt gcacgtaaaa aatgggaaca
     6421 caaataatac taaagtaaat ttaaatacgg catctgtatc agaattgatg tctgttcctg
     6481 gagtagggca agctaaagct aatgcaattg ttgaatatcg caaccaacaa ggtgcatttc
     6541 aagaaattga cgatttgaaa aaagtaaaag gttttggaag taaaactttt gataaactga
     6601 attcttattt cacgacataa tggattattt taattgcaaa ttttattttt tgtgattaat
     6661 cttatattat tagaaaatgt tactaaattt taagtatgga aggtgaacat cttggaaaga
     6721 atcaaatggg aagaatattt tatggcacaa agtcatttgc tagcattacg ttcaacttgt
     6781 caaagattat ctgtaggtgc aacgattgtt aaggataatc gtattattgc tggtggttat
     6841 aatggctctg tagctggcga ggtgcattgt atagatgaag ggtgtttaat tgaagatgga
     6901 cattgtatca gaacgataca tgcagaaatg aatgctttat tacaatgtgc aaaacaaggt
     6961 gtatctactg aaggtgcaac aatctatgtt actcattttc catgcctaaa ttgtacaaag
     7021 tcaattattc aagcaggtat aaagcgtatc tactatgcag aagattatca taaccatgaa
     7081 tatgcaacta aattactcaa acaatctggt attgaattta aaaaaattcc attttcacct
     7141 gaatatgttg ctgaatatct gactaaaggt tgatgttttg ctgtatgtcg cgttatcaat
     7201 gattgtagga gtgctttgga attctagcaa agtgctctct acatttcttt ttattttact
     7261 tttgtatatt gcttatcgta aaaataaaat cgtatatgcc cctatttctc tctttttaat
     7321 cattttctcc tcatggtatt tacattattc acaacaagca atatttaatt atatcaatta
     7381 tattgaacgt aattctcagt ttaatgagcg tgctcaagta atccaaattc aacgtcaagg
     7441 tagtgacaca tataaaggta ggttgagttt aaaaaatgaa atatatcctt tctttttaac
     7501 aaataaaaag aattttgatt taaagaaaat tgaaagtcgt aattgtattg ttaaaggaca
     7561 attcaaagtt aatgacaata agtttgtaac tcttaaatta caaagtatag ttgtacaaag
     7621 ctgcctagaa tcgaaccggt ctaatttaat tgagaaacat aaacagttta taatgaatcg
     7681 aatttatgat tcgggtatta agtttccgga tcgtattatg gcattgatta ctggtgacgt
     7741 aaaagaaatt aatgagcaat ttaaggaacg tgttaaagaa ataggtatat atcatttgct
     7801 ggcagttagt ggctcgcata tagctgcaat tgtattctta atttaccaac ctttaaaacg
     7861 attaaattta cctttatttg tcattaaagg aattacaatc attgtattag ccttatttgc
     7921 tcaatacaca aattatgcac ctagtgctgt aagagctata ataatgacaa cgcttgtact
     7981 gcttattact aatcaaatta aaataaaggg tattcagctt ttagcatttg catttataat
     8041 tatgtttatt ttaaatccac tagttgttta tgatattgga tttcaatttt cattcatcat
     8101 ttcatttttt attatgttac tttttccttt tttacagcaa ttgtcgaagt tacaatcatt
     8161 attcataatt acgtttattg cacaattagc ttcatttatc gttgccattc caaactttca
     8221 tcaacttcaa tgggtgggat ttttatctaa tttgattttt gtaccatact attcgattat
     8281 attgtttcct ctatctattt tattctttat tacaagtcat tttattgtgg gattaacgcc
     8341 gctaaattac ttggttgacc taagttttaa ttttcatgac tggttactag acctattcac
     8401 aagaatcaag caatcacatt tttctgttcc caagtttaat gattggatat ttataatatt
     8461 tataatttcc gtttattaca tattttggtt attggctaaa cgtaaatata tattggttac
     8521 gttttggact ataattattc tgacattatt aataacgttt ccaacaaatt cacatcacaa
     8581 aattacaatg ttaaatgtgg ggcagggaga cagtatttta tatgaaggtg gtaagaacca
     8641 aaatgtcttg attgatacag gtgggaaagt gattgatgat actaaacaac ctagttattc
     8701 aatttctaaa tatcatattt taccaacgct aaatgagaga gggataaatg aattagagta
     8761 tctaatttta acacatccac acaatgac
//
LOCUS       DAKSQE010000103         8713 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.103, whole genome shotgun sequence.
ACCESSION   DAKSQE010000103 DAKSQE010000000
VERSION     DAKSQE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8713
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.103"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..89)
                     /locus_tag="O0V33_002130"
     CDS             complement(<1..89)
                     /locus_tag="O0V33_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976751.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLS"
     gene            complement(131..2662)
                     /gene="secA"
                     /locus_tag="O0V33_002131"
     CDS             complement(131..2662)
                     /gene="secA"
                     /locus_tag="O0V33_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCX8976752.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKSVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
     gene            complement(3076..3648)
                     /gene="raiA"
                     /locus_tag="O0V33_002132"
     CDS             complement(3076..3648)
                     /gene="raiA"
                     /locus_tag="O0V33_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCX8976753.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
     gene            complement(3709..4383)
                     /locus_tag="O0V33_002133"
     CDS             complement(3709..4383)
                     /locus_tag="O0V33_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCX8976754.1"
                     /translation="MNNCLSCGAKLYENITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKYLDQNEAYCLDCKFLSAHFNLMEQLYCQFQYDGLMKEMIHQYKFSKDYYL
                     CELLAHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPVEAVLKAKGIRFDKILKMSNRPK
                     QSHLTKKERLADENPFIIDTELDLNGKEILLVDDIYTTGLTIHRAGCKLYAKNIRKFK
                     VFAFAR"
     gene            complement(4376..5458)
                     /locus_tag="O0V33_002134"
     CDS             complement(4376..5458)
                     /locus_tag="O0V33_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCX8976755.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRM
                     RQTILLYAVTGAGKTEMMFQGIQYARRQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSSQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKSLQQALKSSSK
                     VEHATIYMTATPPKQLLSEIPLENIIKLPARFHKKSLPVPKYRYFKLNNNKIQKMLYR
                     ILQDQINNQRYTLVFFNNIETMIKTFSIYKQKITKLTYVHSEDVFRFEKVEQLRNGQY
                     DVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPTGKVLFFHEGV
                     SMNMIQAKKEIQKMNKLALKRGWIDE"
     gene            complement(5822..6688)
                     /gene="fakB1"
                     /locus_tag="O0V33_002135"
     CDS             complement(5822..6688)
                     /gene="fakB1"
                     /locus_tag="O0V33_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HCX8976756.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQDDCPSAYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            6832..7473
                     /locus_tag="O0V33_002136"
     CDS             6832..7473
                     /locus_tag="O0V33_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCX8976757.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDIGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            complement(7637..8692)
                     /locus_tag="O0V33_002137"
     CDS             complement(7637..8692)
                     /locus_tag="O0V33_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCX8976758.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKEE"
BASE COUNT         2778 a         1581 c         1226 g         3128 t
ORIGIN      
        1 aaactaagta cataacaacg tttaacaact tcttcacatt ttttaaagta tttaacgctt
       61 gtaaaataaa aagactcctc ccataacaca aactataggt gtttaattgg aaggagttat
      121 tttatatcat ttattttcca tggcaatttt tgaatttttt accactacca catggacaat
      181 catcgttacg accaacttga tcgcctttaa cgattggttt cggtttcact ttttctttac
      241 catcttcagc tgaaacgtgc ttcgcttcac caaactcagt tgttttttca cgttcaatat
      301 tatcttcaac ttgtactaca gattttaaaa tgaatttaca agtatcttct tcgatatttt
      361 gcatcatgat atcaaataat tcatgacctt cattttgata gtctcgtaat ggattttgtt
      421 gtgcataaga acgtaagtga ataccttgac gtaattgatc cattgtgtcg atatgatcag
      481 tccaatggct atcaatagaa cgaagtaaaa tcatacgctc aaactcattc atttgttctt
      541 ctaagatatc tttttgactt tgatatgctg cttcaatctt agcccaaacg acttcgaaaa
      601 tatcttcagc atctttacct ttgatatcat cctctgtaat gtcaccttct tgtaagaaaa
      661 tgtcattaat gtagtcgatg aatggttgat attcaggctc gtcatctgct gtatttatat
      721 agtaattgat actacgttgt aacgttgaac gtagcattgc atctacaact tgagagctgt
      781 cttcttcatc aataatacta tttctttcgt tatagataat ttcacgttgt ttacgtaata
      841 cttcatcgta ctctaagata cgtttacgcg cgtcgaagtt attaccttct acacgttttt
      901 gtgctgattc tacagctctt gataccattt ttgattcaat tggtgtagag tcatctaaac
      961 ctagtcggct catcattttc tgtaaacgtt cagaaccaaa acgaatcatt aattcatctt
     1021 gtaatgataa atagaagcga ctatcccctt tatcaccttg acgtccagaa cgaccacgta
     1081 actggtcatc aatacgacga gattcatgtc gttctgtacc tattaccgct aaaccgccta
     1141 attcctctac gccttcacct aatttaatat ctgtaccacg accagccatg ttagtggcaa
     1201 tagtaacggc acctttttgt ccagcgcctg caacaatttc agcttcacgt tcatgatttt
     1261 tcgcatttaa cacatcatga cggataccgc gttttttaag taaatttgaa atatattctg
     1321 aagtctcaac tgcaacagta cctaatagca ctggttgccc tgccttgtgt ttttctacaa
     1381 catcttctac tactgcatca aatttacctt tttggctaat gtaaattaaa tcagacttat
     1441 cgttacgttg cacaggttta tttgtcggaa tttgagttac tgtcatgtta taaatatttc
     1501 taaattcttc ttcttcagtt ttagctgtac ctgtcatacc cgcaagttta ttgtacattc
     1561 tgaaatagtt ttggaatgta atagacgcca tagttttaga ttcattttga atttgaacgc
     1621 cttccttcgc ttcaatagct tggtgtaaac cttccgagaa acgacgtcct ggcattgtac
     1681 gtcctgtaaa ttgatcgaca attaatactt cgccatcaac aaccatatag tctacatcac
     1741 gttgtaatgt aacgtgcgca cgtaaagctg tgttgatatg actaataaca tcaacatttt
     1801 gtacatcata taagttttca actttgaaca tacgttcagc tttatccgca ccttgttctg
     1861 ttaaatgtac tgattttgtt ttttcatcat atttataatc ttcgtcctgt tttaacattt
     1921 ttgcaaaaac atttgcttgt gtataaagtg acgttgactt ttcagcttca ccagaaataa
     1981 ttaatggcgt acgtgcctca tcaattaaaa ttgagtcaac ttcatcaatg attgcgaaat
     2041 gtaatggacg cattactcta tcttcagaat aattcaccat gttatctctt aaatagtcaa
     2101 aacctaactc attgttagta ctgtaagtaa tgtcttgcgc atatgcttca cgtttttctt
     2161 cagtcgtctt actgtttaag tttaatccga cagtcaaacc taagaagtta tataactcag
     2221 ccatttcttc actttgaaca cttgataagt attcattgac tgtaataacg tgaacacctc
     2281 taccagctaa tgcatttaag tatgttggca ttgtcgctgt taacgtttta ccttcacctg
     2341 ttctcatctc agcgatatca cctttatgaa ttgcaatacc acccataatt tgaactttat
     2401 atggtgtcat attgaataca cgtttagagc cttccctaac aagtgcatat gcttctggta
     2461 aaattttatc taaataatca ttttgctttt tgacattatc aatgtcagct aattctgttt
     2521 ggaattgttt cgttttatta cgaatttctt catcagttaa aattgccgtt ttttcttcta
     2581 aagcgattac tttatcagca agtttaccta actgtttaat ttctttatta ttgccatcaa
     2641 gaatttttga taaaaatccc atttcgttcg ctcctttagc taaaaaactg tttggcctac
     2701 aacaatatat cttatcattt atagttagaa aattatactt atttactcat ttgtagaatc
     2761 aatataaata tatttatgac atacttcatt cacattctgt tgtcaacaag tttatcacta
     2821 ataaatatat tctcaaaacg caattatact tcttaataaa ttatattata aatattttac
     2881 gattttcgac tcggactata caatagactg acatactatt attaacttaa cattcaaata
     2941 tatacatcca ttaacattag catagtcact atgtttcatt caacaaatta cattatcgaa
     3001 ctatgaaatt gttagaaatt gcttttagag tataaaaaag cacttgtgca aaaacacaag
     3061 tgctttaaac ttaatttatt gttcactagt ttgaatcaag ccatatttac cgtctttacg
     3121 gcggtaaacg atacttgttc catcagtttc tctgtctgtg aatacaaaga agtcatgacc
     3181 taatagattc atttgtaata ccgcttcttc tgaatccatt ggttttaagc tgaattcttt
     3241 tgaacgaata atttctatct cgttatcatc gtaagcgtca ttatcaactt gtgtttcttg
     3301 catttcttgt aattcggcaa caaacacttc ttgatctcct cgatcacggc tcttacgatt
     3361 aatacgtgtt ttatattttc gaacttgtct ttcaagttta ttattaatta aatcaatacc
     3421 tgcgtataaa tcatcgtttc gctcttcagc tcttaacgta acatttttca atggaattgt
     3481 tacttcaatt ttagtagctg aatttgaata agttttaact ttaacatgcg ccactgcatt
     3541 tggtacgtca ttaaaataac gttccaactt accaattttt tcctcaatat agttgcgaat
     3601 agcatctgtg atagtgaggt tatctccatg aatttcaaat ctaatcatag taatctctcc
     3661 ttaaacctct ttattggtaa ctctttatta tattttaaca tttttacgct atcgtgcaaa
     3721 cgcaaaaact ttgaattttc tgatattttt agcatataat ttacaccctg cacgatgaat
     3781 tgttaatcca gttgtataaa tatcgtcaac gagtaatatt tccttaccat ttaaatctaa
     3841 ttccgtatca ataataaatg gattttcatc tgccagacgc tctttcttag ttaaatgaga
     3901 ctgttttggt ctatttgaca tctttaaaat cttatcaaag cgaatccctt ttgcttttag
     3961 tactgcttct accgggttaa atgttctaga taaatcatgt gccggcgaag aaggaattgg
     4021 cacaatatag tcataagatg tttgtggtat ttcaatcaaa tgtgccaata attcacataa
     4081 ataatagtct ttcgaaaatt tatactgatg tatcatctct ttcattaaac cgtcatattg
     4141 aaattgacaa tataattgtt ccattaaatt aaagtgtgcc gatagaaact tgcagtctaa
     4201 acaatacgct tcattttgat ctaagtattt taagcacctt gaacatcgcc ttgctttaat
     4261 atcaagttta atattgtccc aattctcttt gcatctgtca cataatctat taggtttctt
     4321 gaacaaatta taaatggtta tattttcata taacttagca ccacaactca aacaattatt
     4381 catcaatcca acctcttttt aatgctaatt tgttcatctt ttgaatctct tttttagctt
     4441 gaatcatatt catacttaca ccttcatgaa aaaacaatac ttttccagta ggacattcta
     4501 attttcgtcc aacacgtcca gcaatttgta ttaaagcctc ttgagtatat tgatgtgcat
     4561 cgataacaac aacatctaaa tttgccattg taaatccacg ttctaatatt gtcgtagtaa
     4621 aaatgacatc gtattgtcca ttccttaatt gttcaacttt ttcaaagcga aaaacatcct
     4681 cgctatggac atatgttaat ttagtaattt tctgcttata aatcgaaaat gttttaatca
     4741 ttgtttctat attgttaaaa aacaccagtg tataacgttg attattaatt tgatcttgta
     4801 aaattcgata taacattttc tgaatcttat tattgttaag tttgaaatag cgatatttag
     4861 gaactggaag tgatttttta tgaaagcgag ctggcaattt aattatattt tcgaggggaa
     4921 tctctgacag aagttgtttc ggtggtgttg ctgtcatata aatcgttgca tgttcaactt
     4981 tagaagatga cttcaatgct tgttgtaaac ttttatccat tgataaagga aaggcgtcga
     5041 cttcatcaat aaaaatagta tcaaagtgct gtttgaatcg gtaaagttga tgcactgtgc
     5101 atacaacaaa atgcccttca aattgttgac ttgattgctg gtgtagtatg tctatatctt
     5161 cattaagaaa tgcgtcttta atacgtttac taatttctac aacaacatct acacgtggtg
     5221 acacaatagc tatattatct ccctgtcgtc ttgcatattg aatgccttga aacatcattt
     5281 ctgtcttacc tgcacctgtt actgcatata acaaaatcgt ttgtctcatt ctaatggctc
     5341 gaacaatatg ttctgaggca taagactgtt gttccgataa ttcaaatgag agatgataat
     5401 atgcttgtga tgaactttgc gaactttctg ttattttata tcttgttaca ttatccattc
     5461 gacccagtga tatacaattt ctgcaatata caattttctt ggaatgtact gaggaatcat
     5521 actgataaaa atgctttgga tttgtacttt cacattgaac acaataatag tttgactttt
     5581 gtttgatgac acctgttact tctttattaa ttttttcatt agtaagtgaa gtatgaggat
     5641 gaattaattt cccgtaatgt ttaatgctta aaacctcatt tcaacgtaaa aaagagtcta
     5701 atattataat gattttctta tatctttagc attttgacat tcaaatgatg aacatttaaa
     5761 gccatgtata cgagagataa tttccaatta caatatagac tcttttatta aaattttata
     5821 attatgttaa tcttattttt ctgccaacat agcctaaacc taatccacct gaacctaaat
     5881 gtgctgcaac aactggacca aactcagagt atgctacttg ataagctgaa ggacaatcat
     5941 cttgtaactt tttgtataac gcttgtccat cttcgaaatg gtctccattt atgacaaata
     6001 aagttacttc ttcaaagtct tttacaatat ctaatacttt cttttctaat gtttgaatgg
     6061 cacgcttttt agtacgaact ttttcttctg gtataatctt gccatcttca aacttaagaa
     6121 ctggcttcat tttcaataat gttccaaccc atgcttgagc accagtaatt cgaccacttt
     6181 tttgtaaatt ctttaagtca tcaacaatta aatatgcacc tgtgtgttca cgcatattag
     6241 ttaaatcatc aataatttgc tgtggttcgt atccttcttc aaccatttca atagcacgta
     6301 atacataaca tccttcaatc attgctgcaa gcttactatc aaatgcatgt acattaacac
     6361 cttcaaccat ttcccctgct tgataactag attgataact tccactaatt ccacttgata
     6421 agcaaatgac aatgatatct gtgtaacctt gatctcttaa catttcatat ttagtaatcc
     6481 attcgccaat tgctggttgg cttgttgttg gaatcgtttg agacgatgcc attttattat
     6541 aaaattcttc aattgctatt tcattacttt ctgtaaagtt cttcccatca tcaaaagtca
     6601 cacttaatgg cgctatttga atattatatt tatcgattaa gtcctgcgac agataacttg
     6661 tagaatcggt catcacagca attttcatag tcgtcctcct aaaatatact catagcaatg
     6721 atacaatatt tcagttaaaa agaaaatggc aatcaaacga tttatagtca tatcattttc
     6781 agtgtataat gaagattaaa taatcttaag cttatgaatt aaggagttat tatgacacaa
     6841 aatatcatta ctgttaaaaa agaacacatt attgaaaatg tgattagtaa atcaagattc
     6901 attgctcata tcaaacctgt tcaaaatgaa gatgaagcta aagcatttat tgctgcaata
     6961 aaaaaagaac ataaagatgc cactcataat tgttctgcat atactattgg acctgaaatg
     7021 aatattcaaa aagcgaatga tgatggtgaa ccgacaggaa ctgccggtgt cccaatgtta
     7081 gacatattaa aaaagctcga tgttcataat gcgtgtgtcg tagtaacaag atattttggt
     7141 ggtattaaat taggtggtgg cggtttaatt cgcgcctata gcggtgctgt cagagacgtc
     7201 atttatgata tcggacgtgt cgaactacga gaagcagtac catgtatagt aacactgaat
     7261 tatgatcaaa ctggtaaatt tgaatatgaa ttagcctcaa ctcattttat gttaagaaac
     7321 caaacttaca ctgataaggt aagctactat atcgatgtcg ttaaaagtga ctatgaagcg
     7381 tttattaatt ttttaaatca aatgactgct ggaaatttcg atttgactga agaaactcca
     7441 aagcagttac ctttcgatat accaactgaa taaaaataaa aagcactagc ttaggatggc
     7501 taactctaat tgcgtattgt tactttgcaa tttggaaagc acgttccatg ctagtgtttt
     7561 ttgtgagtat gccttcttta taacaacata aaagtacaga catcattttt tattttatta
     7621 cttaccgttt taaaatctat tcctctttat gagatgactt acgactaatt aaatttaata
     7681 ttggtcggta gttgttatct attaatcctg taaattcaac aattaattca atactaaata
     7741 tgattaatac aaacattaat acaacaccta atggtggcga tacatacaag ataatgctcg
     7801 aaagactaaa taaaatagaa attgaataga ttaataatac cgtttgtcta tgtgtgtagc
     7861 ctaaagctaa taacttatga tgcaaatgcg atttatcagc ttgcattata tgctgccctt
     7921 ttttcacacg tcgaatcatt gcgaacaagg tatcaatgaa tggaactgct aagataacaa
     7981 ttgggaagaa caatgcaata attgtaatat ttttgaatcc gagtaaagaa aggaatccga
     8041 tgataaatcc aatcattaaa gcgccactat cacctaaaaa tattttggca ggatggaaat
     8101 tgtaaaataa aaacccaatt aaagagccta ataaaacaca acaaatcatc gttatgaaaa
     8161 tattagcttg taaaattgca atgaatccta ttgtaatgag tccgattgca gaaacacccg
     8221 atgccaaacc atcgagtcca tcaattaagt taattgcatt tgtaatagca acaatccaaa
     8281 tcacagtaat tggaatacta agaaatccaa aatgaatagt tgtacccatt ggcaacgaaa
     8341 taaaatcaat agtcacacca taaaaagcaa ctactaaggc agcagcaatt tgaccagcca
     8401 attttatata cggtttcaaa tcgtagatat catctacaag cccaagtacg tacataataa
     8461 tcgcaccaat aataagtggt ttgatttctg tttcaatagg atgaccaatc caaataccta
     8521 ttaaaaatga aaagagaatc accgtaccac ccataactga aataggttta gtgtgtactt
     8581 ttctaaaatt tggtttatca actaaattta atcttttcga tattgcaata acaattggtg
     8641 ttatcgtcaa actgacaatc attgttactg caactagtaa taatgtaacc atttattcac
     8701 cttcatcgat att
//
LOCUS       DAKSQE010000104         8240 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.104, whole genome shotgun sequence.
ACCESSION   DAKSQE010000104 DAKSQE010000000
VERSION     DAKSQE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8240
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.104"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            130..981
                     /locus_tag="O0V33_002138"
     CDS             130..981
                     /locus_tag="O0V33_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCX8976759.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNSDKHAGETLTLSTGLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEVVKLNFAGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
     gene            complement(1215..1409)
                     /locus_tag="O0V33_002139"
     CDS             complement(1215..1409)
                     /locus_tag="O0V33_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCX8976760.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENEGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
     gene            complement(2225..3046)
                     /locus_tag="O0V33_002140"
     CDS             complement(2225..3046)
                     /locus_tag="O0V33_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCX8976761.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGSKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGNVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            complement(3064..3759)
                     /locus_tag="O0V33_002141"
     CDS             complement(3064..3759)
                     /locus_tag="O0V33_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8976762.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWTVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWLTNKLDKR"
     gene            complement(3752..4777)
                     /locus_tag="O0V33_002142"
     CDS             complement(3752..4777)
                     /locus_tag="O0V33_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCX8976763.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIILITHEMHVIRRICDEVAVMESGK
                     VIEHGPVTQVFENPQHAVTKRFVKEDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSIDFGKFEKELIERQVK
                     MEVLRHG"
     regulatory      complement(4866..4969)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(5027..5323)
                     /locus_tag="O0V33_002143"
     CDS             complement(5027..5323)
                     /locus_tag="O0V33_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCX8976764.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIYGYTPTCGTCKVSERMLDITNEI
                     LQLPLLKIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAFQSVTDLLENLK"
     gene            complement(5316..5702)
                     /locus_tag="O0V33_002144"
     CDS             complement(5316..5702)
                     /locus_tag="O0V33_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCX8976765.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSNDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFICKKETPFLPNINNERLVLVNE"
     gene            complement(6293..7171)
                     /locus_tag="O0V33_002145"
     CDS             complement(6293..7171)
                     /locus_tag="O0V33_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HCX8976766.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMDLKEMQ
                     EETLNQSKIGGMPFLKSFKDIPLDENNVPMVLLAQINLNDLPEQQELFPVNEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLKDIQAHLKSLDADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDDLALWNKVNPSFALKSMFGGYDELMEPVCNTFTAKEP
                     FNQLGGYPYFDQIDPRTNDQELKMYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTD
                     LEAMKFDDYMYSWDCS"
     gene            complement(7349..7729)
                     /gene="gcvH"
                     /locus_tag="O0V33_002146"
     CDS             complement(7349..7729)
                     /gene="gcvH"
                     /locus_tag="O0V33_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCX8976767.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVATIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQLEALLTAEKYSEMIGE"
     gene            complement(7887..8240)
                     /locus_tag="O0V33_002147"
     CDS             complement(7887..8240)
                     /locus_tag="O0V33_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCX8976768.1"
                     /translation="MKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHIPTINEFKTI
                     IANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDKI
                     TLGFKEDQYKETWLA"
BASE COUNT         2547 a         1508 c         1141 g         3044 t
ORIGIN      
        1 gataataaga acacataatt tctcaatgga ttttatgaat ttaaaatatg ttatcatttc
       61 actaggacat ttgtaatatg gtatgatgct atttatgatt ttattccaat cgtttgaaag
      121 gagttattca tgcaacaatt taaatggatt aatattctaa aaggttttgc tatgggaacg
      181 agtgacttgg tacctggtgt tagcggtggg actattgcat tattgttagg tatatacaat
      241 caatttatcg cttcaattag tggcatattc tcacgacgtt tttggccaag ttttacattt
      301 ttaatcccta ttataattgg aatgttactg gcaatgggat cactaagtaa tctttttaat
      361 tatttattaa gccaacacca tattccaact atgtttttct ttggtggatt aatcattggt
      421 atcgttccat atttattgaa aatttctaac tataaaacgt catttacaac taaacattac
      481 atgatggtta tagctggtat cgctatctta attgttatta cattaatgaa taatagcgat
      541 aaacatgctg gagaaacact tactctatca actggtctta ttattaaata ctttattgca
      601 ggtatgtgtg catctagtgc tatgttactc ccaggtattt cagggtcatt tatgttatta
      661 gtatttggcg tctatggtac ggtcatgcta gcaatatctg aagttgttaa acttaacttt
      721 gccggtcttc ctatattact tgcagtcgga tttggcgtgc ttgctggatt cattatttca
      781 agcaaaatta tccagtattt cctaacacat cataaactaa tgacttttgc attaatcatt
      841 ggttttgttg ttggctccct ctttgcagta tttccaggtt taccaactaa tatcgtgatg
      901 tggttcgttt cattagtagt atttatcatc ggttttattg taagtttgac tttaggtcga
      961 attaccgctg aaaatgaata atcattttag gggtgataaa atatattcag tatctcgtta
     1021 atcatatgca atatatgtct cgttttgtat acatattatt ggatatcatt aacgagtttt
     1081 tatttagttt ttttctaaca ttaaaataat tattaataaa ttttggtatc cactttttta
     1141 atacaaaaaa ccacctagcc gatggttata ccgactaagt ggcatgtgca gtgtattagg
     1201 aatataaagt aaaattactc tcctttgtta ccttttactt tatcaataaa atcagttgct
     1261 ttttcttttg cattttcaac gaattctttc gctttaccag aagctttatc ttctttacct
     1321 tcgttttcta aatttttatt atcagtaaca ttacctactg tttctttaac attacctttt
     1381 gcttgttcaa atttactttc gtctgccata ataaatgcct cctcggaata attaaatgtt
     1441 atatataata cttacccact gaaaaattat ctaaacattt tacttaaata atttttgata
     1501 ttgatttgac gtcattttat aactagcgaa atagattcat cattaacttg agggagcgga
     1561 actaaaataa taaagaatca ctaatgattt attatgaaat ggttcttggg agtgggactg
     1621 aaatgatatt ttcgcaaaat ttatttcgtc atccaacccc aacttgcatt gtttgtagaa
     1681 tttctttttg aaattctctg tgttggggcc ccgccccccc aacttgcaca ttattgtaag
     1741 ctggcttttc gtcagcttct gtattgggac caaccccaac tcgcattgtt tgtagaattt
     1801 cttttcgaaa tcctctgtgt tggggccccg ccaacttgca cattattgta tgctggcttt
     1861 ccatcagctt ctatgttggg cccaacccca actcgcattg cctgtagaat ttcttttcga
     1921 aattctctat gttggggccc cgcaattatt gtaaaaaggt tgttacaatc gtattttcat
     1981 tcaatcaact actgcttata tatcattgta aagctttgga cattgattga agtcacagaa
     2041 gcgtataggc ttcacttaat acataatgac ttgataatca tttatatata gttattcatc
     2101 tcttatcata ttaaatcttc tacatttcat attgattcta attaaaaaaa acccccatca
     2161 aagtttgcat tttttcaatg tctactttga tgggagcata tcagtcaatg agcttaatta
     2221 ttaattattt agcaggaata actgcaccgt tgtatttttc attaatgaag tcttgaatat
     2281 ctttagattg taatacttca attaatgctt tgattttctt atcatcttga tgtccttctt
     2341 taacagcaat taagtttgca taaggattat ctttcgcact ttctacagca atagaatctt
     2401 ttttaggatt tagtttttgt tcgattgcaa agtttgaatt aatgataaca gcatccgcgt
     2461 cttcattttg ataaatttta ggtaagaatt ctgctgattg tttattatta aacttaatat
     2521 cttttttatt ctctgtaata tcactaaact tagcatcttc aatttttacg ccttttttga
     2581 tcttaattaa acctgcatca acgaagaatt ttaagaagcg tccttgttca gctggattat
     2641 tagacacata aactgttgca ccttttggta attcttttaa acttttatac tttttagagt
     2701 atacagccat aggttctaag tgaacattac cggcacttac gattttgtaa cctttatcct
     2761 ttttctctgt gtttaaatat ggtgtatgtt ggaaatagtt tgcgtcaatt tcacctttgt
     2821 ctagtaattt attaggcgta gtgtaatcgt taattgtttt aatatctagt tcataacctt
     2881 ttttctctaa taatggtttt gctttttcta aaatttcagc atgtggtgct ggtgaagcac
     2941 ctactgttat tttcttgtcg tcactgccac ttttgcttcc attaccgcaa gctgctaata
     3001 caactgcaaa tgttaatact aaaataagac caaataattt tttcataaaa tgaaaccccc
     3061 aatttatcgt ttatcaagtt tatttgtaag ccaatcccca atgaattgga ttataaatac
     3121 aataattaaa ataaaaactg ttgatactaa aatgacatca ttttgatttc gagtgaaacc
     3181 tgttaagtat gctaaatttc ctaaaccacc ggcaccaatt acacctgcaa ctgccgttga
     3241 accaactaaa gcgattgctg taactgtaat gccagacact agcgctggca tggcttcagg
     3301 taagaggact ttacgaatta ctgtccaagt attagcaccc attgaccagg ccgcttcgat
     3361 gacaccttta tcaatttctt taaaagcaat ttctacgagc cttgcataaa acggcgctgc
     3421 gccaatgatc aatgctggta acgcacctgt cggaccactt atcgttccaa gtatcaaact
     3481 tgtaaatgga attaataata aaattaaaat aataaatggt atcgctctaa ataagttaac
     3541 aatgaaagaa acgatagaat aaaataacct tgcaccaata gacttacctt tagcagacaa
     3601 gaataataac acacctaaaa taagaccgag tataaatgca aatatagttg agacgactgt
     3661 catgtatagt gtttcgacta ttgcagtcca aacttctggc cactgaatat taggcattgt
     3721 aatcatttca tttataattt cactaaatga tttacccatg tcttaacacc tccattttaa
     3781 cttgtcgctc aattaactct ttttcgaatt ttccgaaatc tatacttgaa atatatggaa
     3841 tatgcagaac taaaaagccg actgttccat tttttgtatt tttaatattt gcttctaaaa
     3901 tattaatttt aatatcatag gcagttgata gactcgatac aataggctcg gttgttgttg
     3961 aaccagcgaa aactaatcta acgatataag catctttttc taatggctct aattctgtta
     4021 aagatgtttc gaaatcatca tttaaatctt ctttcacaaa tcgttttgtc acagcgtgtt
     4081 gcggattttc aaaaacctgt gtcaccggtc catgttctat cactttacca ctttccataa
     4141 ctgcaacttc atcacaaata cgacgaatga catgcatttc atgcgtaatt agtataattg
     4201 ttaaattttg ttgttctcta atttttagta gtagatctaa aatttcatct gttgtttgcg
     4261 gatcaagtgc acttgttgcc tcatcacaaa gcaagactgt tggatcatta gctaacgctc
     4321 gtgcaatccc aacgcgttgc ttttgtccgc ctgataactc tgatggataa gccttttctc
     4381 taccttttaa accgacgagt tcgacaagtt ctaatgcttt ttgctttgct ctccttctag
     4441 ggacacctgc aatttcaagc ggaaacataa tattttttaa cacagtcctt gaccataaca
     4501 aattaaaatg ttggaagatc atacttactt tttgtctttt tgctcttaat ccatttttgg
     4561 acaattggcc tatatggtct ccatctataa taacttcacc tgatgtaggc gcttctaaat
     4621 gattaaacat tcgaatcaaa gtactttttc ctgctccaga aaaaccaatg acgccataaa
     4681 tcgatcctgc tcgaatcgat aaattaacgt gatctacagc aaggacttct ttatttttag
     4741 tccgatattc tttaacaact tcttttaatt caatcacgtt gattcctccc tgtgttgctt
     4801 aataaaataa aaaatgcttt ctcaatatcg atagaaaaat tgagaaagca atagtagtat
     4861 tgtttctctc atcttcaaaa gttaaaactt tatgtgaatt ggcaccattt ctatataaga
     4921 cggttgccgg gcttcgtagg gcacatccct ccaccactct cgataagagt ttacgcatca
     4981 tttaatttgt attaatccta acaccttagt aaaatttcgt caataactat tttaaatttt
     5041 ctaacaaatc agtcaccgat tgaaatgcat aaattcgttt tacttcttta tctttattca
     5101 tcaacaataa aatcggcgta gacatgattt gcatatcttt acaaaactga ggataaaagt
     5161 ttaaatctat tttcaataat ggcaactgca atatttcatt agtaatgtct aacattcttt
     5221 ctgaaacctt acacgtacca caagttggtg tataaccata gattaaatgt ttatcttcct
     5281 cataaaatgt agttacatct ttgatgtcta atgaattatt catttactaa aactaacctt
     5341 tcattattta tattcggtaa gaaaggtgtt tctttcttac aaataaagcc atgttttgaa
     5401 agtacatgcg ccaaatattg tttggggcaa ttcgcaactt gacagtaagt tttatcaata
     5461 aatatatgtt cactttcact caaataacgt ttaaaccaat ttctaattcg atctccttcg
     5521 tcattagaat cggctaatac aaaaacttgt ttatcatata gtgattctat catatcatca
     5581 agcttatcta tactcattgt tccatgagta caaataatat tgactggttc tgcaataacc
     5641 tgttgcaccc tttttttatc agatttccct tcaacaatta tcactttatt tacaatagcc
     5701 atcatcatca ccctttaaaa tcaataaaca tctgtcactg tatcatttca caaaattggt
     5761 atgaataaaa cataaatcac aaaaaattta aactagctta atataataat tacaaaatca
     5821 atgtttgact agctgggaca tttaacataa gcagacaaag gctaaaccat aaatcaacat
     5881 cctaaaatct acaatgatat attaccaata gttaaccaaa agaaaataca cctataacaa
     5941 gcctttttca attatagcgg ggccccaaca tagaagctga cggaaagtca gcttacaagc
     6001 aatgcaagtt ggggtgggcc ccaacacaga agctgacggt aagtcagctt acaagcaatg
     6061 tgcaagttgg ggtgggccca aacataaaga tatacttttt ctttagaaat tagtatttct
     6121 tatgcatgag ttttactcat gtattcctat ttttaagtac acattagctg tggcttatga
     6181 aaaagaacca ctacattata aatcactagt ggttctttat catttctgtc ccactcccta
     6241 gattgatcta cagatactac acttattaaa gtaatatatt tttatgattc tcttagctgc
     6301 aatcccatga atacatgtaa tcatcaaact tcatagcctc aaggtcagta gattttacta
     6361 agatattggc aatacctaaa tcaccccata taatcgaaga attaccatct cttgtagaat
     6421 caatttgcag taagactcta tcatacattt tcagttcttg atcgttcgtt cttggatcta
     6481 tttggtcaaa atatggataa ccaccaagtt gattaaatgg ttccttagca gtaaatgtgt
     6541 tacacacagg ttccattaat tcatcataac caccaaacat tgattttagc gcgaaggatg
     6601 gattgacttt attccacaat gcaagatcgt cctcatcgta cttataatca gtacatgtaa
     6661 tagtttgttt cgacaatcta aattctatag aaaatgctcc gctgaacggg atatcctcat
     6721 tatcagcatc taatgacttc aaatgcgctt gaatatcttt gagtgataaa tctgtaattg
     6781 gctcttttat ataaacaagc cttgagttta tattgtttcc ctttaaattt tcagacatac
     6841 catacatttg atcttctgaa ctaatccaaa actgcaatat cccttcattt acaggaaata
     6901 attcttgttg ttctggaaga tcattcaaat taatctgtgc taacaatacc attggtacat
     6961 tattttcatc taatggtata tctttaaaag attttaaaaa tggcattcca ccaatcttac
     7021 tctggtttaa tgtttcttct tgcatctctt ttagatccat ttcaatacat ggtagtactg
     7081 gcaaattacc gttttctaca gattctacga aacggttata tgctttttgc tcctttttag
     7141 aaagatgact aacgtcttga atgtttagca tatttactcc cccaattttt atttttaaaa
     7201 tagctaaatt cattattttc atcattcttg aataataaaa tgtagcaata ctccaaataa
     7261 tagtacctaa taaattttct ttcatattat ttacttgctc agactaaaaa taaactcctc
     7321 aatcttagat taaggagtta cacggtgatt attcaccaat catttctgaa tatttttcag
     7381 ctgttaataa agcttcaagc tgactttcat cactaatttc tacttttacc atccatgctt
     7441 tttcgtatgg agattcattt acaaattcgg gactatcttc tagttcttcg ttgacttcaa
     7501 ctactttacc agagattggt gcatataatt ctgatacagt tttaactgat tctacgctac
     7561 caaacgtatc cccttcatta atttcatcat ctgtttctgg taattcaaca aaaacaatat
     7621 cacctaactc gctttgtgcg tattctgtga ttccaattgt tgctacatta ccttcaactt
     7681 taacccattc atgctctttt gaatatttca attcatttgg tactgccaag agaatcccct
     7741 cctaattaat ctttacatca ttaatgatgc cataaggcta gatgctattc aactatattt
     7801 ataaaaatta acaattgtat agcttatttc attttacatg taatgaatct aaatatcgag
     7861 atatttagta aacgtttaca ttatacttac gctaaccaag tctctttata ttgatcttct
     7921 ttaaatccta atgttatctt atcgcccatt actgctagag gacgctttac taacatacca
     7981 tcagatgata acaactctaa cttttcatca tctgataaag tttgtaattt atttttcaaa
     8041 tcaagctcac gatatttcgc accgtgtgta ttaaacaatt tattaatttc tacgcctgta
     8101 tttgcaatta ttgttttaaa ttcatttatt gtaggtatat gttgaacgat atcaattggt
     8161 tcataactta cgccatattc atctaaaaac tttgctgcct ttttacaagt tgtacaattc
     8221 ttatattggt aaaatttaat
//
LOCUS       DAKSQE010000105         8142 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.105, whole genome shotgun sequence.
ACCESSION   DAKSQE010000105 DAKSQE010000000
VERSION     DAKSQE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.105"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            198..329
                     /locus_tag="O0V33_002148"
                     /pseudo
     CDS             198..329
                     /locus_tag="O0V33_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
     gene            387..1106
                     /gene="splC"
                     /locus_tag="O0V33_002149"
     CDS             387..1106
                     /gene="splC"
                     /locus_tag="O0V33_002149"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplC"
                     /protein_id="HCX8976769.1"
                     /translation="MNKNLVIKSMAALAILTSVTGISAAVVEETQQIAKAEKHVTQVK
                     DTNVFPYHGVVAFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKI
                     KSISDYPGNEDISVMNIEEQAVERGSNGFNFNDNVQAFKFAKDAKVDDKIKVIGYPLP
                     AQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKVGSE
                     YNGAVYFTPQIKDFIQKHIEQ"
     gene            1230..1946
                     /locus_tag="O0V33_002150"
     CDS             1230..1946
                     /locus_tag="O0V33_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCX8976770.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVSTTMVEGIQQTAKAEHNVTKIT
                     NTSISPYKGILRVGAATGFVVGKNTILTNRHVVKDVQVGSTVLAHPNGENDTGGYYKV
                     KKVIPYAGSADLAIVEVEEDSVYPKNKKFSENTEILTLASEAKANERVAIVGYPAPYK
                     NKHHMYQSTGTVLSINGDKLVSDAFAEGGNSGSPVFNNKNEVVGVLYSGDQIGHSKKE
                     SYSVYLTPEIKKFIADNTEK"
     gene            2113..2828
                     /locus_tag="O0V33_002151"
                     /pseudo
     CDS             2113..2828
                     /locus_tag="O0V33_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038688.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
     gene            2992..3708
                     /gene="splE"
                     /locus_tag="O0V33_002152"
     CDS             2992..3708
                     /gene="splE"
                     /locus_tag="O0V33_002152"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplE"
                     /protein_id="HCX8976771.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVGTTMVEGIQQTAKAEHNVKLIK
                     NTNVAPYNGIVSIGSGTGFIVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKV
                     KKIVRYAGKEDIAILHVEDKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYI
                     NKFQMYESTGKVLSVKGNMIISDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPANKSTK
                     GYGVYFSPEIKKFIADNLDK"
     gene            complement(3657..3774)
                     /locus_tag="O0V33_002153"
                     /pseudo
     CDS             complement(3657..3774)
                     /locus_tag="O0V33_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500438.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3878..4597
                     /gene="splF"
                     /locus_tag="O0V33_002154"
     CDS             3878..4597
                     /gene="splF"
                     /locus_tag="O0V33_002154"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HCX8976772.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVGTTMVEGIQQTAKAENSVKQIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGSPILNSKYEAIGVIYAGNKPSGEST
                     RAFAVYFSPEIKKFIADNLDK"
     gene            4662..4796
                     /locus_tag="O0V33_002155"
                     /pseudo
     CDS             4662..4796
                     /locus_tag="O0V33_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500435.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4960..6516
                     /locus_tag="O0V33_002156"
     CDS             4960..6516
                     /locus_tag="O0V33_002156"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCX8976773.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     CFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKVELIDQVGYFIEPQDLFSAMIH
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVT
                     DGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYEN
                     FDIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADF
                     AFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGIIRRYLIEEKNYLESVIGLPANIFYG
                     TSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKKTIDK
                     YSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAY
                     LKELGVLKDE"
     gene            6509..7663
                     /locus_tag="O0V33_002157"
     CDS             6509..7663
                     /locus_tag="O0V33_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCX8976774.1"
                     /translation="MSNTQTKNVPELRFPGFEGEWEEKKLGDLIKVNSGKDYKHLEKG
                     DIPVYGTGGYMTSVSEPLSEIDAVGIGRKGTINKPYLLEAPFWTVDTLFYCTPKKETD
                     ILFILSLFRKINWKVYDESTGVPSLSKQTINKINRFVPSNKEQQKIGEFFIKLDRQIE
                     LEEQKLELLQQQKKGYMQKIFSQELRFKDENGNDYPNWEEKKIEDIASQVYGGGTPNT
                     KIKEFWNGDIPWIQSSDVKVNDLILRQCNKFISKNSIELSSAKLIPANSIAIVTRVGV
                     GKLCLVEFDYATSQDFLSLSSLKYDKLYSLYSLLYTMKKISANLQGTSIKGITKKELL
                     DSIIKIPHNLEEQQKIGDLFYKIDKYISFNKCKIEILKSLKQGLLQKIFI"
     gene            7700..>8142
                     /locus_tag="O0V33_002158"
     CDS             7700..>8142
                     /locus_tag="O0V33_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976775.1"
                     /translation="MEKLNEAIKNYIDSDELFALFIDGQWGVGKTYYIKNKILKEFDD
                     KIEIRYISLYGLQNLYEIKSIIISELINTSTKNLRKVLKSMKNFQVVPYLNMNNFSDN
                     ILRYIDDNSLKKIKNSLMKNNKSALLIIDDVERMSDKVSFEEFLG"
BASE COUNT         3190 a         1203 c         1428 g         2321 t
ORIGIN      
        1 gaaagttgag gattttcttc tatatttgtt aactttgatg ttactaaatg taatattaac
       61 ttaccataat tagcattatt tgacatttat gtttataact tttcaagtaa gaatataact
      121 ttacaagtcg ataattaata tcataattat atggaataat agtcatgata gggagaattg
      181 tgacatgcat acacaaccta aatagcaaca acgaactagt aggtattcat tttgcttcag
      241 atgtgaaaga tgatgacaat agaaatgctt atggcgtcta cttcacacca gagattaaaa
      301 aattcattgc agaaaatata gataaataat caaattgttt taaaacgagc gttgcaatat
      361 atctcaaatt gtaaaggagc ttgaaaatga ataaaaattt agtcattaaa agcatggcag
      421 cattagccat tttaacatca gtaactggaa taagtgctgc agtcgttgaa gagacacaac
      481 aaatagcaaa agcagagaag catgttacgc aagttaaaga tacaaatgtt tttccgtatc
      541 atggcgtcgt tgcatttaaa gatgcgacag gctttgtaat cggtaaaaat acaattatca
      601 ctaataaaca tgtatcaaag gattataaag ttggcgatag aattactgct catccaaatg
      661 gtgacaaagg aaatggtggt atttataaaa ttaaaagtat ttccgattat ccaggcaatg
      721 aagatatttc cgtcatgaat atagaagaac aagcggttga acgtggatcc aatggcttta
      781 attttaatga taatgttcaa gcatttaaat ttgcaaaaga tgctaaagtt gatgacaaaa
      841 ttaaagttat cggttacccg ttacctgcac aaaacagttt taaacaattt gaatctacag
      901 gaactataaa acgaattaaa gacaatattt taaattttga tgcatatatc gaaccaggta
      961 attcaggatc acccgttcta aattctaaca atgaagtcat aggtgttgta tatggcggca
     1021 ttggaaaagt tggttctgaa tacaatggtg ctgtatattt tacgcctcaa attaaagatt
     1081 ttattcaaaa gcacattgaa caataaacat atttaaatat acaccatgag catgtgttca
     1141 ataattttaa tgaaaaacat cggtcgaata taacataaaa agcaaacgtc tatatcaaaa
     1201 gcatcatgaa taaacagagg agcacaaaaa tgaataaaaa tataatcatc aaaagtattg
     1261 cggcattgac gatattaaca tcagtgactg gtgttagcac aacaatggtt gaagggattc
     1321 aacaaacagc caaagccgaa cataatgtaa cgaagataac aaatactagt atttcgccat
     1381 acaaaggcat actaagagta ggcgcagcta caggatttgt agtaggtaaa aacactattt
     1441 taacaaatag acatgtcgtt aaagatgtgc aagtaggtag tacagtgctt gcccatccaa
     1501 atggcgaaaa tgatactggc ggatactata aagtcaaaaa agttatccca tatgcaggct
     1561 cggcagattt agctatcgtt gaagttgaag aagattcagt ttacccaaaa aataaaaagt
     1621 tcagtgaaaa tactgaaatc ctaacgttgg catcggaagc taaagcgaat gaaagagttg
     1681 caatcgttgg ctatccagcg ccatataaaa ataaacatca tatgtatcaa tcaacaggaa
     1741 cagtactatc aattaatgga gataaattag tatcagatgc atttgctgaa ggtggcaatt
     1801 cagggtcacc ggtctttaac aataaaaatg aagttgtggg cgttttatat agtggagacc
     1861 aaattggtca ttctaaaaaa gagtcataca gcgtatattt aacgcctgaa attaagaaat
     1921 tcatcgcaga taacacagaa aaataagccg gaaagctcac ttacatgcct caacttagat
     1981 tacattttta aacttaataa ctaattcaac tatcatcata tctctgtgat tctatttatt
     2041 cgaaataatt ttaaaaataa aacttcaaaa acctaacctt atattaatac gaatacttag
     2101 aggagcacaa aaatgaataa aaatataatc atcaaaagta ttgcggcatt gacgatttta
     2161 acatcagtga ctggcgtcgg cacaaccatt gttgaaggta ttcaacaaac agcaaaagcc
     2221 gaacataatg tgaagcaagt acaaaacaca aatatttctc cgtataaagg aattgttaga
     2281 attggatcag ctacaggatt tgtggtagga aaaaatacaa ttttaacaaa taagcatgta
     2341 ttacctggtg ttcaagttgg tagcacaatt cttgctcatc caaatggcga aaatgatacc
     2401 ggtggatact ataaagtaaa aaaatcattc catacgcagg atctgaagat ttagcaatag
     2461 ttcaagttga agaagattct gtttatccaa aaaataaaaa attcagtgaa aatactgaag
     2521 tccttaaatt tgcgagtggc gctcaagtaa atgatcgttt atcaatcgtt ggttacccta
     2581 atccatataa aaataagcat catatgtttg aatcaacagg aaagatatta tccatcgatg
     2641 gtaatcaaat gacttcagat gcatttgctg aaggtggcaa ttcaggatcc ccagtgttta
     2701 attcaaatca tgaggtcatt gcaattgcat atgctgtcga tgtcaaaaat gatgcaacaa
     2761 aaaaatcata tttagtttat ttcacacccg agatcaagaa attcattgca gataacacag
     2821 aaaaataaac gaacatacaa atccatcctt acattgataa ataatttttt aaaataaaca
     2881 acaaactcaa caattcaaat catatctctg tgattctatt tattcgaaat gattcaaaaa
     2941 aactcaaaaa cctaacatta taattataag aatacttaga ggagcacaaa aatgaataaa
     3001 aatataatca tcaaaagtat agcagcattg acgattttaa catcagtgac tggtgttggc
     3061 acaacaatgg ttgaaggtat tcaacaaaca gccaaagccg aacataatgt gaaactaatc
     3121 aaaaatacta atgtagcacc atacaatggt atcgtttcga taggatctgg aacaggtttc
     3181 attgtcggaa aaaatacaat tgttactaac aagcatgtcg ttgcaggtat ggaaattggc
     3241 gcacatatta ttgcgcatcc taatggtgaa tataataatg gcggttttta taaagttaaa
     3301 aaaattgtcc gttatgcagg taaagaagat attgccattc tacatgtgga agataaagct
     3361 gttcatccaa aaaataggaa ttttaaagat tatacaggta ttttaaaaat agcatcagaa
     3421 gccaaagaaa atgaacgcat ttcaattgtt ggctatccag aaccttatat aaataaattt
     3481 caaatgtatg aatcaacagg aaaagtgcta tcagtgaaag gcaacatgat tatttctgat
     3541 gctttcgtag aaccaggcaa ctcaggttca gctgtattta atagtaaata cgaagttgta
     3601 ggtgttcact ttggtggaaa cggccctgca aataaaagta caaaaggata tggcgtttat
     3661 ttctctcctg aaattaagaa attcatcgca gataatttag ataaataatt aagcagataa
     3721 atcaatcctt acataagata gatgattttt aaaaattaac aacaaaatca acaattcaaa
     3781 tcatatctcc gtgattctat ttattcgaaa tgattaaaaa aataaaactt caaaaaccta
     3841 accttatatt aatacgaata attataggag cacaaaaatg aataaaaata taatcatcaa
     3901 aagtattgca gcattgacga ttttaacatc agtgactggt gttggcacaa caatggttga
     3961 gggtattcaa caaacagcca aagccgaaaa tagtgtaaaa caaattacaa atacaaatgt
     4021 tgcaccatac agtggggtta catggatggg cgcaggaaca ggatttgtag ttggaaatca
     4081 tacaatcatt actaataaac atgttaccta tcacatgaaa gtcggtgatg aaattaaagc
     4141 acatcctaat ggtttttata ataatggtgg tggactttat aaagttacta agattgtaga
     4201 ttatcctggt aaagaagata ttgcggttgt gcaagttgaa gaaaaatcaa cacaaccaaa
     4261 aggtagaaaa ttcaaagatt tcactagcaa atttaatata gcatcagagg ctaaagaaaa
     4321 tgaacctata tcagtcattg gttatccaaa tcctaatgga aataaactac aaatgtatga
     4381 atcaactggt aaagtattat cagtgaatgg gaatatagtg tcatcggatg caataatcca
     4441 gcctggtagc tctggatcac ctatattaaa tagtaagtac gaagctattg gtgtaatcta
     4501 tgcaggtaat aagccatcag gtgaaagcac aagagcattc gctgtttatt tctctcctga
     4561 aattaagaaa ttcattgcag ataatttaga taaataatta aaacttagac attcacccaa
     4621 tcctgacaaa atatactata actaacattt attaatatat attgcattat ttaatatgca
     4681 tcaaagccaa tcaatgattg attttcacca actcaattgt tgattggttt tatttatgta
     4741 tgaatgaaca actttttgac atcattgaga atataaatga ttttgaaagc atttgaaagc
     4801 tacaacattt ccaaaaaaat tttcaataac aattgcgccg ctaaaactca aaatttccac
     4861 aaccaacatc aaaattctaa acatcgcaac aaaaccaaat gttataataa atctattaca
     4921 caaagagata aattacttat gcaaaggcgg aggaatcaca tgtctattac tgaaaaacaa
     4981 cgtcagcaac aagctgaatt acataaaaaa ttatggtcga ttgcgaatga tttaagaggg
     5041 aacatggatg cgagtgaatt ccgtaattac attttaggct tgattttcta ttgcttctta
     5101 tctgaaaaag ccgaacaaga atatgcagat gccttgtcag gtgaagacat cacgtatcaa
     5161 gaagcatggg cagatgaaga ataccgtgaa gacttaaaag tagaattaat tgaccaagtc
     5221 ggttacttca ttgagccaca agatttattc agcgcgatga ttcatgaaat tgaaacgcaa
     5281 gatttcgata tcgaacatct ggcgacggca attcgtaaag ttgaaacatc aacactaggt
     5341 gaagaaagtg aaaatgactt tatcggactg ttcagcgata tggacttaag ttcaacgcgt
     5401 ttaggtaaca atgtcaaaga acgtactgcg ttaatttcca aagttatggt taatcttgac
     5461 gatttaccat tcgttcacag tgatatggaa attgatatgt taggtgatgc atatgaattc
     5521 cttatcgggc gctttgcggc gacagcgggt aaaaaagctg gcgagttcta tacaccacaa
     5581 caagtatcta agatactggc aaagattgtc acagacggta aagataaatt acgtcacgtg
     5641 tatgacccaa catgtggttc aggttcatta ctgttgcgtg ttggtaaaga gacacaagta
     5701 tatcgttatt tcggacaaga acgtaacaat accacataca acttagcacg catgaacatg
     5761 ttgttacatg atgtacgtta tgagaacttt gatatccgta atgatgatac gttggaaaat
     5821 ccagcctttt taggcaatac atttgatgcg gttattgcga acccaccata cagtgcgaaa
     5881 tggacagcag attcaaaatt tgaaaatgat gaacgtttta gtggttacgg caaacttgcg
     5941 ccaaaatcca aagcagactt tgcctttatt caacacatgg tacattactt agacgatgaa
     6001 ggtaccatgg ccgttgtact cccacatggt gtcttattcc gtggtgccgc agaaggtatc
     6061 attcgtcgtt atttaattga agaaaagaac tacttagaat ccgtgattgg tttgccagcg
     6121 aatattttct atgggacaag tattccaaca tgtattttag tatttaaaaa atgtcgccaa
     6181 caagacgaca acgtattatt tatcgatgca tccaatgatt ttgaaaaagg aaaaaatcaa
     6241 aaccatttaa gcgatgccca agtcgaacgt attatagaca catataagcg taaaaaaacg
     6301 attgataaat atagctacag cgcgacatta caagagattg ccgataacga ttacaactta
     6361 aacataccgc gatatgtcga tacattcgaa gaagaagcgc caattgattt agatcaagtc
     6421 caacaagatt tgaaaaatat cgataaagaa atcgcagaaa ttgaacaaga aatcaatgca
     6481 tacctgaaag aacttggggt gttgaaagat gagtaataca caaacgaaaa atgtgccaga
     6541 gttgagattc ccagggtttg aaggcgaatg ggaagagaaa aagttagggg atcttataaa
     6601 agttaattct ggaaaagatt ataaacattt ggaaaaaggt gatataccag tctatggtac
     6661 tggcggttat atgacaagtg tttcagaacc actaagtgaa attgatgctg ttggtattgg
     6721 gagaaaaggg actataaaca aaccatattt gcttgaggcg ccgttttgga cggtggatac
     6781 attattttat tgtacaccta aaaaagaaac agacatacta tttatattaa gtttatttag
     6841 aaaaataaat tggaaagtat acgatgaatc aacaggtgtg ccaagcttaa gtaaacaaac
     6901 cattaataaa ataaatagat ttgtcccttc aaataaagag cagcaaaaaa taggcgaatt
     6961 cttcatcaaa ctcgaccgac aaattgaatt agaagaacaa aaacttgaat tacttcaaca
     7021 acagaaaaaa ggctatatgc agaaaatctt ctcacaggaa ttgcgattca aggatgagaa
     7081 tggaaacgat tatccgaatt gggaagagaa gaaaatagaa gatatagcaa gccaagtata
     7141 tggaggcgga acaccaaata caaagattaa agaattttgg aatggagata ttccatggat
     7201 tcaaagctct gacgtaaaag taaatgattt gattctacga caatgtaata aatttatttc
     7261 caagaattca attgagcttt cttctgcaaa acttattcct gccaattcaa ttgcaatagt
     7321 tacaagagtc ggggttggaa aactgtgttt ggtagaattt gattatgcta caagtcaaga
     7381 ttttttatca ttaagtagtc ttaaatatga caaattatac tcattatatt cattgctata
     7441 tacaatgaaa aaaattagcg ctaatctaca aggaacttca attaaaggta taacaaaaaa
     7501 agagttgtta gatagtataa taaagatacc ccataatcta gaagaacagc aaaaaatagg
     7561 tgatctattt tataaaattg ataaatatat cagttttaat aaatgtaaaa ttgagatact
     7621 taaaagtctc aaacaaggat tacttcaaaa aatttttata taaatgatat agaatttaaa
     7681 attttgtaga ggtgaattga tggaaaaatt aaatgaagca attaaaaact atatagatag
     7741 tgatgaattg ttcgcccttt ttatagatgg acaatgggga gtagggaaaa cttattatat
     7801 taaaaataaa atattaaagg aatttgatga taaaatagaa attaggtata taagtttata
     7861 tggcctgcaa aacttatatg aaattaagag tataatcata agtgaattga taaatacatc
     7921 tactaaaaat cttaggaaag tcctaaaaag catgaaaaat ttccaagtag taccttattt
     7981 aaatatgaat aacttttctg ataacatatt acgttatatt gatgataatt cattaaaaaa
     8041 gataaaaaat tccctgatga aaaataataa aagtgctttg ctaataatcg atgatgttga
     8101 aagaatgagt gacaaagtaa gttttgaaga atttttggga tt
//
LOCUS       DAKSQE010000106         8021 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.106, whole genome shotgun sequence.
ACCESSION   DAKSQE010000106 DAKSQE010000000
VERSION     DAKSQE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.106"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..76
                     /locus_tag="O0V33_002159"
     CDS             <1..76
                     /locus_tag="O0V33_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCX8976776.1"
                     /translation="AMRSIKKLADGSMKRYEALKRIKV"
     gene            88..666
                     /locus_tag="O0V33_002160"
     CDS             88..666
                     /locus_tag="O0V33_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCX8976777.1"
                     /translation="MKTESYFKEYNQFVIDQQKAIQELKQERNALESKIKIDKSTYKQ
                     LIMDGQDDKADNLYQATDADEKKLKALNKRLETKKSVSKEVKYQKTIELLKHQSELSS
                     LYGSEKQSALGKLKKVVDAYNEIIDEIEDINDRYEDEHQQYASIYSQEQLYDDKEARE
                     ALNGYFRENIFTSYINGNDLPYEHNNKLFLKR"
     gene            684..902
                     /locus_tag="O0V33_002161"
     CDS             684..902
                     /locus_tag="O0V33_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid morphogenesis B protein"
                     /protein_id="HCX8976778.1"
                     /translation="METKYELNNTKKVANAFGLNEEDTNLLINAVDLDIKNNMQEISS
                     ELQQSEQSKQKQYGTTLQNLAKQNRIIK"
     gene            953..1480
                     /locus_tag="O0V33_002162"
     CDS             953..1480
                     /locus_tag="O0V33_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HCX8976779.1"
                     /translation="MKLLKTKNCLYYRNGDNKLSEYQLLTQFNPAFINKKIKMCEFQI
                     ESMYHMSASTTTCDEIMGVVSVSYPIEKLVIKIIETKAGLQNYKNRSINNMALLKKVL
                     NHYTEKEQKQVVKYMRSNGRYKPYNVIERLQVDLYQASIKQRSERQKQRNTAIENSKI
                     ARVNAYHQSSYVKVV"
     gene            1483..1824
                     /locus_tag="O0V33_002163"
     CDS             1483..1824
                     /locus_tag="O0V33_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCX8976780.1"
                     /translation="MDKKQIKDFVCDYHKRTRSDVLIDDEINTDEFFSIGDENSNEWM
                     ADDNIDDHIVKNHLEMIVDQVANDKEFYIFDSLIQGRSFKDISNVLECSEQSVRLWYE
                     TLLDKIVEVIE"
     gene            1821..2288
                     /locus_tag="O0V33_002164"
     CDS             1821..2288
                     /locus_tag="O0V33_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002500560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCX8976781.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYSANSAHVTGCRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNAAVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKARDMLGKYHKLFTDKHDINGNVPIFINIG"
     gene            complement(2545..3249)
                     /gene="tst"
                     /locus_tag="O0V33_002165"
     CDS             complement(2545..3249)
                     /gene="tst"
                     /locus_tag="O0V33_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_111772164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic shock syndrome toxin TSST-1"
                     /protein_id="HCX8976782.1"
                     /translation="MNKKLLMNFFIVSPLLLATIATDFTPVPLLSNQIIKTAKASTND
                     NIKDLLDWYSSGSDTFTNSEVLDNSLGSMRIKNTDGSISLIIFPSPYYSPAFTKGEKV
                     DLNTKRTKKSQHTSEGTYIHFQISGVTNTEKLPTPIELPLKVKVHGKDSPLKYWPKFD
                     KKQLAISTLDFEIRHQLTQIHGLYRSSDKTGGYWKITMNDGSTYQSDLSKKFEYNTEK
                     PPINIDEIKTIEAEIN"
     gene            3668..3895
                     /locus_tag="O0V33_002166"
     CDS             3668..3895
                     /locus_tag="O0V33_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HCX8976783.1"
                     /translation="MKQNDLAHGEFGNWLKNINLDRTQAHRFIKVSEEIKDVGTYQHL
                     GLRALSEIASLPVPVRIKVHITSNGKTKIPY"
     gene            complement(4750..5685)
                     /locus_tag="O0V33_002167"
     CDS             complement(4750..5685)
                     /locus_tag="O0V33_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCX8976784.1"
                     /translation="MRLQKAPLVTSGLILGLLGLGNLLKDISLVLNAICGIFALFIWV
                     HLLCTILNNFKNVKEQLNTPLVSSVFTTFFMSGFLGTTYLNTYFSDVTIITSLITPLW
                     LLCLMGIMIHMIIFSIKYLKGFSLENVYPSWTVLYIGIAIAGLTAPISGFYLIGKLSV
                     IYGFLATCIVLPIVFKRLKTYPLQTSIKPNTSTICAPFSLVAAAYVITFPKANDLIVI
                     ILLVLAQFFYFYIIFKLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLTYPEIWEW
                     LLFFEITLATLIVLRVFLGYVHFFFKTSPKASYNS"
     gene            6804..6983
                     /locus_tag="O0V33_002168"
     CDS             6804..6983
                     /locus_tag="O0V33_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976785.1"
                     /translation="MATISITTDYKFTKKSAQKLIDAMEINENNSNVNKTNIKATKIK
                     STCEIENLLKDYRSN"
     gene            6980..7276
                     /locus_tag="O0V33_002169"
     CDS             6980..7276
                     /locus_tag="O0V33_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976786.1"
                     /translation="MTVKVISLSELLTGDKQEVKRKIPSVLNILNSFETISISGSESA
                     HDVDLFLKNKSIAFDRQNLSRTHLVFSQFKNKQILVGYFTISNKPLVFYKTYVR"
     gene            7266..7607
                     /locus_tag="O0V33_002170"
     CDS             7266..7607
                     /locus_tag="O0V33_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976787.1"
                     /translation="MLDKISNTLKKKLYQKGETHSGNDNLIIQGYLIAQIEKNYSEEA
                     LATKSINANDLLTLAYVKVLEGANIFGGSYIWIEYEDVDRLREFYRKFGFTEIKDHTS
                     ENNLKMAILKI"
BASE COUNT         3068 a         1009 c         1377 g         2567 t
ORIGIN      
        1 agcgatgaga agtattaaga aattagctga tggcagtatg aaacgctatg aagctttgaa
       61 acgaattaaa gtgtaaaggg gataaaaatg aaaactgaat cgtactttaa agaatacaac
      121 caatttgtaa tagatcaaca aaaggctata caagaattga aacaagagcg taatgcattg
      181 gagagtaaaa taaagataga taagtccaca tataaacagt taatcatgga tggacaagat
      241 gataaagcag ataacctata tcaagcaaca gatgctgatg aaaagaaact aaaagcactt
      301 aataaacgct tagagacaaa gaaaagtgtg tcgaaagaag ttaaatatca aaagacaatt
      361 gaattattaa aacatcaaag cgagttgtca tcattatatg gatcagaaaa acaatcagct
      421 ttaggtaaat taaaaaaggt agtcgatgca tataatgaga tcattgatga aatagaagat
      481 attaatgata gatatgaaga tgagcatcag caatatgcga gtatttatag tcaagaacaa
      541 ttatatgatg ataaagaggc tagggaagca ttgaatggct actttagaga aaatatattt
      601 acatcatata ttaatggtaa tgatttgcca tacgaacaca ataacaagtt gtttttaaaa
      661 cgttaaaaag aaagggtaat taaatggaaa caaaatacga gttaaataat actaaaaagg
      721 tcgcaaatgc atttggttta aatgaagaag atacaaatct attaataaat gcagttgatt
      781 tggatattaa aaacaatatg caggagattt caagtgagtt acaacaatca gaacagtcta
      841 agcaaaagca atatggtaca acgctacaaa atttagctaa gcaaaacagg attattaaat
      901 agcaatgatt gcctatccaa ttcgggtagg ctctgtttat aggggtgaat aaatgaaact
      961 gcttaaaacg aagaattgtt tatattatcg taatggcgac aataaactat ctgagtatca
     1021 actattaacg caatttaacc cagcatttat taataaaaaa attaagatgt gtgaattcca
     1081 aattgaaagt atgtaccata tgagtgcgtc gaccacaaca tgtgatgaaa taatgggggt
     1141 cgtgtctgtc tcatatccga ttgaaaaatt agttatcaaa attattgaaa caaaagcagg
     1201 gttacaaaac tataaaaata gatctataaa taatatggcg ttgttgaaaa aggtactaaa
     1261 tcattataca gaaaaagagc agaagcaagt tgtaaaatat atgcgttcaa atggacgata
     1321 taagccttac aacgtcattg aacgcttaca agttgatttg tatcaagcaa gtattaaaca
     1381 acgttcagaa cgtcaaaaac aaagaaatac agcaattgaa aacagtaaga ttgcacgagt
     1441 aaatgcatat caccaatctt catatgtaaa agtggtgtaa caatggataa aaagcaaata
     1501 aaagacttcg tttgtgatta tcataagcga actagaagtg atgtgttgat agatgatgaa
     1561 ataaataccg atgaattctt ttcaataggt gatgaaaatt ctaatgaatg gatggcagac
     1621 gataacattg atgatcatat tgtaaagaat cacttagaaa tgattgttga ccaagtagct
     1681 aatgacaaag agttttatat tttcgattct ttaatacaag gacgtagttt taaagatatt
     1741 agcaatgtct tagagtgttc agaacaatct gtaagattat ggtatgaaac cttattagat
     1801 aaaattgtgg aggtgataga atgagtgagt taacggcaaa acaagcgcgt tttgtgaatg
     1861 agtatataag aacacttaat gtaacacaaa gtgccataaa agcaggctat agcgcaaata
     1921 gtgcacatgt gacaggatgt aggttattaa agaagccaca catcaagcaa tatatacaag
     1981 aacaaaaaga taagattata gatgagaatg tattaaccgc aaaagagtta ctacatgtgc
     2041 ttacgaatgc ggcagtcggt gatgagacag aaacgaaaga agttgtagtc aagcgagggg
     2101 aatataaaga gaatccacaa agtggcaaag tacagctagt ctataacgaa catgttgaac
     2161 tgatagaggt accaataaaa cctagtgatc gtttaaaagc tcgtgatatg ttgggtaaat
     2221 accataagtt atttacagat aagcatgata ttaacgggaa tgtgcctata ttcattaata
     2281 ttggttaatg ggatggcgat gatgaagatt tagataagac ggtacaagag gtatctaacg
     2341 ctaatcctaa tcatactgtg attgtggatg atataccgtt agaggattga ttacagtaaa
     2401 aacgattatc atattgagtt agtgaggatt agtttactaa ttcaccctag ctttatatta
     2461 aagcgttata aagataaaag ggagaacgct tattataatt aacggactcc ctttattaat
     2521 aattattaca gaaaaagtgg taaattaatt aatttctgct tctatagttt ttatttcatc
     2581 aatatttata ggtggttttt cagtattgta ttcaaacttt ttagataaat cactttgata
     2641 tgtggatccg tcattcattg ttattttcca ataaccaccc gttttatcgc ttgaacgata
     2701 taatccatgt atttgagtta gctgatgacg aatttcaaag tctaaagttg atatagctaa
     2761 ttgtttttta tcgaactttg gccaatactt taaggggcta tctttaccat gaaccttaac
     2821 ttttaaaggt agttctattg gagtaggtaa tttttcagta tttgtaacgc cacttatttg
     2881 gaaatggata taagttcctt cgctagtatg ttggcttttt ttagttcttt ttgtgtttaa
     2941 gtcaactttt tccccttttg taaaagcagg gctataataa ggactcggaa aaattataag
     3001 gctgatgctg ccatctgtgt tttttatacg catagatcct aaggaattat ctaaaacttc
     3061 actatttgta aaagtgtcag acccactact ataccagtct agcaaatcct ttatattatc
     3121 gtttgtagat gcttttgcag ttttgattat ttgattagat aataagggaa caggggtaaa
     3181 atctgtagcg attgtcgcaa gcaacaaagg gcttacgata aaaaaattca ttagtaattt
     3241 tttattcatt tttaattctc cttcattcaa atgtgtaaac gtttacatat agaatgtata
     3301 aatattattt aaatgaatca attaaccatc tctaaattat tgtttaaata tatattaatt
     3361 aaaaagtgtt tgttacatag ggagctatat caaaaaatat agatttaaac aacattttaa
     3421 agttacaaat agcaaaaaac aaagtgtgag tggtcattta aagaatatta attaatataa
     3481 gtttgaatta gttatattct ttaaagtcac tttctagtgg cgttttttac gctgagaaac
     3541 gtcctgtgtt gcagtaaggg atgggaacca cgcacaaata ctttaactgt aaacataacg
     3601 tcgtgaaata tggctttaaa catcgctggt caatctatct ttgagattgg tcgaagatta
     3661 aaaccatatg aaacaaaatg acctagcaca tggagaattt ggtaattggt tgaaaaatat
     3721 caacttagat agaacacaag ctcatcgttt cattaaagtt tctgaagaaa ttaaagatgt
     3781 tggtacatac caacatttag gtctgagagc tttatcggaa atagctagct tacctgtacc
     3841 agtacgcatc aaagtacaca taacatcaaa cggcaaaact aaaattccat actaaatgtt
     3901 tatgaattta actgcagaac tcaattttga gccttgtaaa attacataaa ttagttatat
     3961 aaatattatt tatgggtatt atataaacgg gaggggcaac gttattactt gcctattaga
     4021 acatggaatg gttctgcccc aactagtcag gtactaggcg actaatgggg agaaatcagt
     4081 tgaaatgaca tagtcatgtc tatttaagca ggtgtgtaac acacctgctt tctatttaca
     4141 tttaaagata aaatgtgcta ttattttact agaacttttt aacatttctc tcaagattta
     4201 aatgtgcata acaggcaggt acttcggtac ttgcctattt ttatgtaaac atattaggtg
     4261 tatgtataaa tttagggtat tgattataga tacttaatat atggcggaat ggttgaaaaa
     4321 tgataaagtg aggaaagtgt ttgactttca ttagtattag tacttttaat gattttattt
     4381 ttaatgtgta tagtaattta taaaattgaa agttatctaa cagttaagaa aatactaaaa
     4441 caattacaaa taaatattac attgtcttgg attaccaaat ggtaattgaa acctcagatc
     4501 tttagtttaa agctaatttt aataatgcaa acattcaagc aagtttaagg gttggtggat
     4561 aaagagaaaa aaataatagg gtatagaatt aagtttttta ccctataccc agttttatat
     4621 gaagcaagta aaatcggcag ctgaatggct ggttgatttt gagcaaagat ttattaagat
     4681 atgtcttgtc atatttctct ttatcatttt gtcattacta taagatattt ttaaaagtgc
     4741 tacattagat taagagttat agctagcctt cgggctagtt ttaaaaaaga aatgaacata
     4801 gcctaaaaag actcttaata ctattaaagt tgctaatgta atttcaaaaa ataagagcca
     4861 ttcccaaatt tctgggtacg ttagtacagg taaactattt tttaaggcag ttgctgaaat
     4921 tactaaagga aaagtgaaag ctgaaaatac tggtgaaaac ggctctttta gcaactttgg
     4981 aagtttaaat ataatataaa aataaaaaaa ctgagccaat accaaaagaa taataacgat
     5041 tagatcattt gccttaggaa aagttataac atatgccgca gcaactaaag aaaatggtgc
     5101 acaaattgtg gaagtgttcg gtttaataga cgtttgcaat ggatacgttt ttaatcgttt
     5161 gaatactatt ggtaagacaa tacatgttgc taaaaaacca tatataactg ataattttcc
     5221 aattaaataa aatccgctga ttggtgctgt taatccagca atagcaatac caatataaag
     5281 cactgtccat gatggataaa cattttcgag cgagaaccct tttaaatatt taattgaaaa
     5341 aataatcata tgtatcataa tccccataag acataagagc cataagggtg ttattaagct
     5401 agtgataatg gttacatcac taaaatacgt atttaaataa gtggttccca aaaatccaga
     5461 catgaaaaat gttgtgaaca cagatgaaac tagaggggta ttcaattgtt ctttaacatt
     5521 tttaaaatta ttgagaatag tacataaaag gtgaacccaa ataaagaggg caaagatacc
     5581 acaaatagca tttaaaacaa gtgatatgtc tttcaagaga ttgcccaacc ccaacaaacc
     5641 taagatcaat cccgatgtta ctaaaggtgc tttttgaagt ctcatgattt aaaaccttcc
     5701 ttttgtgatt ttattcacta attataacat gatatcttag aactattaat aagcagaatg
     5761 agttttgtat gtttaatgtg gattaatatg atgttgtttc gggaaatata tgtatttatc
     5821 tatttttgat attttatatt cagtataata cgtgattaca acgttgaata taaaaaaata
     5881 tgacgatgtt tatacgtatt aatattaata taatcacacg ttgcaggcat caaacgctta
     5941 tttattaatc aaaaatgggt ggacaaattt atatagtttt atcaatttta atattttaca
     6001 ctaactttat taggtgatat aagatgctga gataagcatt atattgcaat gaaaaagcat
     6061 tatatggata atcatatcta tcattgcaaa tatacttata gagatttatg tgtgtgataa
     6121 ttggtggtca taaattggtc ataatgaaat aaaaaaacta aaaaaattga atgcataaag
     6181 aatacacgat gctgatttaa taggattttt gtatatgatt tatatctatt tcatactgcc
     6241 cttaatgcca ggaatgatgt aaaacagttt ctagttttga ctaactagaa aattacatta
     6301 tgcacgagta tgattcgttc caaataaata ttagagcgta tgaaaatatg tttttaagac
     6361 gttttccatt agttaccaaa acttttggaa ggcgtcttta ttatgtgctg atgtgttatt
     6421 tagattcata atggggggtt ttttcaatgt taaaatcata tagattatat agagaagttt
     6481 agtatacctt ttgagtatat aaggagtttc ttctagtatg ttgtaatgta attagatttc
     6541 cggtaatcaa ttcggctttg cagaggactc acttgcgtat tgtaataaga agctgtctgc
     6601 attttgaaaa cacccacact agttacagca tgggtgtttt tatgctaaga aacaaattgg
     6661 tttaagtgta aaatcatata cactaaagaa atttattgaa tctattgtcg aaaagatgct
     6721 ttcatgatac attgtaaaca agaagacaac gtagacaatg tatacttggt tgtcaaatga
     6781 agtaaaataa ggaagtgagt agtatggcta caataagtat tactactgat tataaattca
     6841 caaaaaaatc agcgcaaaaa ctaatagatg caatggagat taacgaaaat aatagtaatg
     6901 tgaacaaaac aaacataaaa gcgactaaaa taaaatcaac ttgtgagatt gaaaatctat
     6961 tgaaggatta tcgaagtaat tgactgtaaa agtaatatca ctttcagaat tgttaacagg
     7021 tgataagcaa gaggttaagc gaaagatacc ttcagtttta aatatactaa attcatttga
     7081 gacaatatca atttcaggaa gtgaatcagc acacgatgtt gatttatttt tgaaaaataa
     7141 gtctatagca tttgatagac aaaacctgtc tagaactcat ttagtttttt cacaattcaa
     7201 aaacaaacaa atactagttg gctattttac aattagcaat aaacccttag ttttttacaa
     7261 aacgtatgtt agataaaata tcaaacacgt taaagaagaa gttatatcaa aagggtgaaa
     7321 ctcacagtgg aaatgacaat ttaatcatac aaggatactt aattgctcaa atagaaaaaa
     7381 attattctga agaagcattg gctacaaaat ccataaatgc aaacgattta ttaactttag
     7441 catatgtgaa agttttagaa ggtgcgaaca tatttggcgg ttcatatatt tggatagagt
     7501 atgaggatgt agatagatta agagaatttt atagaaaatt tggatttaca gaaatcaaag
     7561 atcatacaag tgaaaataat ttgaagatgg ctattcttaa aatataaagc acaaaaccac
     7621 acccacctat tgatttagaa gtgtggttat ttttatggaa gaatttataa aataaaggtc
     7681 aaagacattt gaatatttat caactgctct tctatcaaca cccatgtgtc tagctatttc
     7741 acttttgctt attttcatgt ttaagttcat cataacaatt attaattttt gtaaatatga
     7801 aagagtagta acttcaaaat ccgtatttat gtctgaagat aatttcattg ttgttcacag
     7861 caataaaatt atctctagaa ttttagaaaa tgtacatgtt taaacaatca aaagtataca
     7921 ttattaaatt atcattttca ttcatctttt caacaattga ggttaacgta gtaaaaaata
     7981 agttttaaat atttaaatca ttgtatagtt tagttttgag t
//
LOCUS       DAKSQE010000107         7869 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.107, whole genome shotgun sequence.
ACCESSION   DAKSQE010000107 DAKSQE010000000
VERSION     DAKSQE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7869
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.107"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            94..1596
                     /locus_tag="O0V33_002171"
     CDS             94..1596
                     /locus_tag="O0V33_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCX8976788.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYL
                     SMYTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSEQSWQTYDYVFLTCGTFA
                     YHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPI
                     TMTSRSANLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECAEYDI
                     DFEKLVHRRTGNHIADLKYDLARPTEMGIFQSIIEYLKENLNWIWNSLSIEDQHQFNQ
                     KYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDI
                     YNVVINATGAKSHLNELDQDDQLIKNLENRQIVQAHPMGGIQIIPETNQVISPRFGTL
                     TNMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQEQRSDNQ"
     regulatory      1815..1950
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2077..3120
                     /gene="ribD"
                     /locus_tag="O0V33_002172"
     CDS             2077..3120
                     /gene="ribD"
                     /locus_tag="O0V33_002172"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCX8976789.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKIAKVVYATKDNS
                     LDTHGDETIRDHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRHTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTTNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDKFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     gene            3127..3759
                     /gene="ribE"
                     /locus_tag="O0V33_002173"
     CDS             3127..3759
                     /gene="ribE"
                     /locus_tag="O0V33_002173"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCX8976790.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFNQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINKQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            3770..4951
                     /gene="ribB"
                     /locus_tag="O0V33_002174"
     CDS             3770..4951
                     /gene="ribB"
                     /locus_tag="O0V33_002174"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCX8976791.1"
                     /translation="MQFDNIDSALMALKNGETIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQKFKEKHKLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHL
                     I"
     gene            4964..5428
                     /locus_tag="O0V33_002175"
     CDS             4964..5428
                     /locus_tag="O0V33_002175"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCX8976792.1"
                     /translation="MNFEGKLIGKELKVVIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAVITLGCVIRGATSHFDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(5550..6551)
                     /locus_tag="O0V33_002176"
     CDS             complement(5550..6551)
                     /locus_tag="O0V33_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HCX8976793.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAAKEFVKPAGLKRVGIIAALGATVMLCLSTIKKLCRK"
     gene            6663..6782
                     /locus_tag="O0V33_002177"
     CDS             6663..6782
                     /locus_tag="O0V33_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976794.1"
                     /translation="MMLISFVYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            6785..7612
                     /locus_tag="O0V33_002178"
     CDS             6785..7612
                     /locus_tag="O0V33_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCX8976795.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVETRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
BASE COUNT         2840 a         1095 c         1443 g         2491 t
ORIGIN      
        1 accttaataa attttattaa aaataccaaa ttgctataaa tatgggtaat agataaaaga
       61 aaatatttaa aatgactaaa ggatggatga tatatgcgtg tagcaataat cggcatggga
      121 actgctggtg taagtgtatt gagagagtta gttaagcacc cgaaatttaa tcaattagat
      181 atagatttat acgatgataa agtaaatatg ggacagggcg taccatttca aaatgatagt
      241 tctgagctgc taattaatat gccttcgaaa aagatgagtt taaatttaga tgatgagact
      301 gaattttgga agtggtataa acaacaaact gattttaatt ttgatgaacc tgcatattta
      361 ccgagatttg tattcggaca ttatatgaaa tcttatttat ctatgtatac gaaaaaatat
      421 ccaaatatat caactaatta taataaagtt caagagattt atacaaattc taatatcgat
      481 gaaacgaatt taacttatta catatgtact acaaattcag aacaatcatg gcaaacgtac
      541 gattatgtat ttttaacatg tggtacattt gcctaccatg atccatataa cttgaagggc
      601 aaaaaaggat atatcgctac gccttatcca acatataata cgttagacga agtaaatgaa
      661 ctggacgata ttgcgattat tggtacaggc cttgcaagtt tagatgttgt tcgttatgtt
      721 gctgctcatc atccgaaatt accaattact atgacgagtc gttctgcaaa tttaccaagt
      781 gtaagaggta caatgataga cgtgacattc aagtacttaa ctaaagacaa attaaatgac
      841 ataaaaaaac atcatttcgg caatgcaccg cttgatacgt tagtttcttt atttttaaaa
      901 gaatgtgctg aatatgacat tgattttgaa aaattagtac atagacgtac aggaaatcat
      961 attgcagatt tgaagtatga tttagcgcgt ccaacagaaa tgggcatttt ccaaagtatc
     1021 attgaatatt tgaaagagaa tttaaattgg atatggaata gcttaagcat tgaagaccaa
     1081 catcaattta atcaaaaata ctcaaagatg attcaattaa attcaaatcc aatgcctcca
     1141 agaactgctg aactaattat tgaattaata gaaaataagt ctttaatttt gaaaaaagat
     1201 ttagaagatg tgaaacatga tggcaagttg tattattttt catacaaaaa ccaagaatca
     1261 gtagacatat ataatgttgt cattaacgcg acaggtgcaa aaagtcattt aaatgaatta
     1321 gatcaagatg atcaattaat taaaaatctt gaaaatagac aaattgtaca agcacatcca
     1381 atgggaggca tacaaatcat accggaaacc aatcaagtta ttagtccaag gtttggaaca
     1441 ttaacaaata tgattgcgat aggtcaaatg acaaacggtg ttaataaact acgaaatggt
     1501 gtgaaaatga tagtagaaca agttgcgcat acagtatctc aattatatga tgctttagaa
     1561 tcgaatgagc aagaacagcg aagtgacaat caataatttg taactagaag ataataaaga
     1621 gaacgctcta tagagacgca ttgaggtttt gattttaatg tctgttagta agaatcatag
     1681 caatgagatg cctatagtac tcagattata ttaaattaaa accgtcatta attgtttttt
     1741 cagaaaacat atagtatcat tttaaatgta gttgacatac tacgtactca aataatctat
     1801 aacaatttca tatataattc tttcggggca gggtgaaatt cccaaccggc agtaaattaa
     1861 gcctgcgacc tgctaatatg tttcgtatta gtggctgatc tagtgagact ctagagccga
     1921 cagttaaagt ctggatggga gaaagaatgt taattatcga caaagataat gtagcgtatt
     1981 tgtaaaaatg tgtacaaata ggcttattta acgataaatt tttctccttg catcttaatt
     2041 catgatgtga ggatttttgt ttatagaggt gatcatttga gtcaatttat ggattatgcg
     2101 attcaacttg caaatatggt acaaggtcaa acaggtgtta atccacccgt tggcgctgtt
     2161 gtagttaaag aaggtaggat tgttggtatt ggtgcacact tgagaaaagg tgacaagcat
     2221 gcggaggttc aagcacttga tatggcacaa caaaatgctg aaggtgcgac gatttatatt
     2281 acgttagagc catgtagtca ttttggttca acgccaccct gtgttaacaa aataattgat
     2341 tgtaagatag ctaaagtagt ttacgcaaca aaagacaatt cgttagatac acatggtgat
     2401 gagacgatac gggatcacgg tattgaggtt gaatgcgttg atgatgaacg tgcatcacaa
     2461 ttataccaag acttttttaa agcaaaagca aagcaactgc cacaaattac agtgaaagta
     2521 tctgcaagtt tagatggtaa acaagcgaat gataatggac aaagtcaatg gattactaac
     2581 aaagaggtta aacaagatgt ctataagtta agacatcgac acgatgcagt gttaactgga
     2641 agacatacag ttgaattaga tgatccacaa tatactacac gtattcaaga tggaaaaaac
     2701 cctataaaag taatattgtc taagtctggg aatattcatt ttaatcagca aatttatcaa
     2761 gatgaatcaa caccaatttg gatatatact gaaaatccaa atttaacaac caatcaaaca
     2821 catattgaaa ttatttactt gaagtcttgt gatttaacaa caattcttca caatttatat
     2881 aaaagaggag ttggaacttt gctagtcgag gcaggtccaa ccactacttc agaatttctc
     2941 caatctaatt atatagacaa atttattctc tattatgccc cgaaattaat tggcggatct
     3001 ggaaattatc aattttatca aacaaatgat gtgattgaga taccagatgc gaaccaattt
     3061 gaaattgttc attccgagtt attaaatcaa aatgtcaaat taactttacg aaagaagtga
     3121 tgatgcatgt ttactggcat cgttgaagaa ataggtgtcg ttaaaagtgt tcaaattcgt
     3181 caatcagtca ggacgattga aatcgaagca cataagatta cggcagatat gcatattggt
     3241 gattccatca gtgttaatgg tgcatgttta acagtgattg attttaatca gacatctttt
     3301 actgtacaag taattaaagg cactgaaaat aaaacctatt tagcagatgt taagcgacaa
     3361 tcagaagtaa atttagagcg tgccatgagt ggtaacggta ggtttggtgg acattttgtc
     3421 ctcggtcatg tagatgaact aggaacagtt tcaaaaataa atgaaacagc caatgccaaa
     3481 attattacga ttcaatgtag ccaacatatt aataagcagt tagtgaaaca agggtctatc
     3541 actgtggatg gtgtaagtct aacggtattt gacaaacatg ataacagttt tgacattcat
     3601 cttattccag aaacgaggcg ttcaacgatt ttatcatcca aaaaattagg agataaagta
     3661 catttagaaa cagacgtttt gtttaaatat gttgaaaata ttttaaataa ggataaagac
     3721 caattatctg tagataaatt aagagcattt ggtttttagg aggggtagca tgcaattcga
     3781 taatattgac agtgctttaa tggctttaaa aaatggagaa acaattattg tagtagatga
     3841 tgagaatcgt gaaaatgaag gtgatttagt agcggtcact gaatggatga acgataatac
     3901 cattaatttt atggcgaaag aagcaagggg attaatatgc gcaccagtgt ctaaagatat
     3961 tgcacaacgt ttggatttgg tacaaatggt tgatgataac tccgacatct ttggtacgca
     4021 atttacagtg agtattgatc atgtagatac aacaacagga attagcgctt atgaacgtac
     4081 attgactgcc aaaaagctca ttgatccaag tagtgaagct aaagatttta atcgtcctgg
     4141 tcatttattt ccattagtag cacaagataa aggggtatta gctcgtaatg gtcatactga
     4201 agcggctgtt gatttagcta aacttactgg tgccaagccc gctggtgtca tttgtgagat
     4261 tatgaatgat gacggcacga tggcgaaagg acaagattta caaaagttta aagaaaaaca
     4321 taaattaaag atgattacga ttgatgattt aattgaatat cgtaaaaaat tagaaccaga
     4381 aattgaattt aaggcaaaag tgaaaatgcc tacagatttc ggaacatttg atatgtatgg
     4441 ttttaaagcg acttacacag atgaagagat agttgtactg acaaaaggtg caattcgaca
     4501 acatgaaaat gtacgcttac attctgcgtg ccttacaggc gatattttcc atagtcaacg
     4561 ttgtgattgt ggtgctcaac ttgaatcgtc tatgaagtat atcaatgaac atggcggcat
     4621 gattatttat ctacctcaag aaggtcgtgg cataggattg ttaaacaaat tacgcgcata
     4681 tgaattaatt gagcaaggat atgatacagt aactgcaaat ttagcattag gttttgatga
     4741 agatttgcga gattatcata ttgctgcaca gattttaaaa tattttaaca tcgaacatat
     4801 caatttatta agtaataatc caagtaaatt cgaaggatta aaacaatatg gcattgatat
     4861 tgcagaaaga attgaagtta tcgtaccaga aacggtacat aatcatgatt atatggaaac
     4921 gaaaaaaata aaaatgggtc atttaatata ggaggacttt aacatgaatt ttgaaggtaa
     4981 attaattgga aaagaattga aagttgtaat cgtagttagt cgatttaatg attttatcac
     5041 tggaagatta cttgaaggtg caaaagatac tttgattcga catgatgtta atgaagacaa
     5101 tattgatgtt gcatttgttc cgggtgcgtt tgaaattcct ttagtagcta aaaaattagc
     5161 ctcatcagga aattatgatg cagtaattac attaggatgc gtaattcgcg gtgctacgtc
     5221 tcattttgat tatgtttgta atgaagtcgc gaaaggtgtt tctaaagtaa atgatcaaac
     5281 taatgtacca gtcatatttg gtattttaac gactgaaagt attgaacaag ctgttgaaag
     5341 agcaggtacg aaagctggca ataaaggtgc cgaagcagca gtaagtgcaa ttgaaatggc
     5401 taatttatta aaatctataa aagcatagag cgctgttgtg agttcataat caaagattta
     5461 taaatgattc agaactgcca atataatatt taggagccta gaacactgat taatgttcca
     5521 gactcctaaa ttattgttta tgccaagctc tatttacggc ataatttttt aattgtactt
     5581 aaacataaca taactgtagc tcctaaagct gcaattatgc caacacgttt taagccagca
     5641 ggtttcacaa attcttttgc agcaagagaa aggttttgtg ggcgttctgc taatcttctc
     5701 ataaaatacg caaaccaatc atcgccataa ggtacataaa tagtgaaatt atatccttca
     5761 tttgcaattt cttctgctaa ctctgatcta aaaccataga gcatttggaa ttccatacga
     5821 tctttttcaa tgtgattttc tttcataaat tgttttacat gattaatgat gcgatggtca
     5881 tgtgttgcaa ttgaagtgaa attgcgtgcg tttaacaaac gttgttcaat tatcttgatg
     5941 taatttgcat ctacgtcttc cttagattga aatgcaattg attcgttttc tttatatgca
     6001 cctttaacca aacgtaatcg taaatcttgg tacttatcaa ctaattcatg gctatcgtat
     6061 aaatatgctt gaattacagt accaacattt ctaaattcgc cttttaatcg atctaaaact
     6121 tgaacaattt gttgcaggct agcatatttt tcagtatcaa tatttatatg catattattg
     6181 taagtatttg ctttaagtaa aatctctctt aaattttggt aagctaattc taagtcgaat
     6241 tctgcaccta actgactcaa tttaacagac atatgtgcct ttacgccatg ttgatgaagc
     6301 gcgtccataa ttgttaaaat ttgttcttta gcatgattac tttcttcaac tgtaccgaca
     6361 aattccccta aattgtctac cgtaacagcg atattcttgt catttaagta ttcgattgta
     6421 ttaattaact ctggaattgt atttccggca acgactttat tggcacccaa acgtgggccc
     6481 actttttttg ctgcgttgtt taaaaaacta ttattagata atccgataaa aaaattcttt
     6541 aatagtgcca ttgctactcc tccaaaccct tgtgtttaca ataagtttag catgaaaaaa
     6601 atattcgaga aagcgttttc atcaaaaaca attaaaaata attatgtata attacactat
     6661 tattgatgtt gatttcattt gtatataata atatggacaa caataatact ataaatagaa
     6721 atctacatag tgaatttgct ataattatgt tattgaagat taatgaaaaa ggggcagtat
     6781 aataatgtgg aagtgggaag ctgaaaatga tgcgaaaggc gtcgttgtta ttgctcataa
     6841 tatattagaa catacgggaa gatatgcata tgttattacg atgttaagaa gaaatggcta
     6901 tcatgtgatt atgggagatt tgcctggtca aggtcaaact tcaagagcta ataaaggaca
     6961 aattgaaaac tttcaaacgt atcacgaaag tttgctagac tggttaaaga tagctaatga
     7021 atataaaatt cctacttatg tattaggggt aggacttggc ggtttgattt tattaaattt
     7081 acttgaaaaa gtagaattgc caatcgaggg gatgatgtta atttctccta tgctagaact
     7141 tcaaaagaat ggaaaaaatc gtaaagataa acttgtttca aatataggta aaatttcaaa
     7201 agacacacgt tttaatgttg gtgtagaacc aaaagattta acacgtaatt tagaaattgt
     7261 tgaagaaaca gttaacgatg gtttaatgtt aaaaaaagca acgtatcatt ggtataacac
     7321 aattaatgag acgatgaaag atacgatggc tcatattcat gacattcaac caatgccaac
     7381 actattaatg tatggaacga aggatttaat tgttgaaact agggcaattg atgagtttaa
     7441 agaaaaatat caaacacctg aattatattt taaagcttgg caaggttttt accacgaggt
     7501 tcacaatgag ccagaacgcg atgaagtaat gcgttatatt ctgactttct taaataatag
     7561 cgtcaataca atgggcttta ttgttgaaga tgacgaaatt gtagaaattt aatattctaa
     7621 aaaggtgaga cagtagatgc tcatcttttt ttaaactttt ttaaggttga gaatgtatat
     7681 cacattttat acacatttga tttgtaagaa atgttttgat ttatgcaaat catatcttga
     7741 aaaataacca atttagtctc attcggtttg atttaatttg ttaaatttaa aggtaactaa
     7801 ttaatttagt ttaatcaatt ttcatgagag ttatatgtaa taaaaattca atgcgtatct
     7861 tttttgagg
//
LOCUS       DAKSQE010000108         7860 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.108, whole genome shotgun sequence.
ACCESSION   DAKSQE010000108 DAKSQE010000000
VERSION     DAKSQE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7860
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.108"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            156..1064
                     /locus_tag="O0V33_002179"
     CDS             156..1064
                     /locus_tag="O0V33_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8976796.1"
                     /translation="MVHLIQISNINKSFKKNRVLKNISFDIEKGTCTALIGKNGAGKS
                     TLVDILSNKIIADDGVILDKDKLLQSENRSIMFQKTMFPDQLKVIGIIKLYQSFYENP
                     LTLDVIIELTKFNSNQLNQFANKLSGGQQRLLDFVISLIGQPQLILLDEPTSNMDIEM
                     REYFWSIIAKLKEENRTILYTSHYIEEVERMSDKIILIENGEIKLNDSTSHIRTNQQS
                     QITLSDEYKRKLKPDKDDLVIQKNHNGTIKIITSNVNDTILYLQQLHINLDDIEIQKV
                     SIVDSYFNNKKQRGSNYDTKLLENRI"
     gene            1033..1764
                     /locus_tag="O0V33_002180"
     CDS             1033..1764
                     /locus_tag="O0V33_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8976797.1"
                     /translation="MILSYLKIEFKVILRKKTTLILSILFPVIFYILFTSILDLPEDV
                     KPKFYKEYMYSMTVYSLLSFSLLTFPLDIINEKQNGWRQRLMVTPFTFTSYYISKVVK
                     TMLQFAIAILVIFMVGHFYKGVEMSAVQWLESGIFLWLGASLLITFGILFSLLNDIQK
                     TSALANIVTIGLAVLGGLWFPINTFPNWLQHVAHVLPSYHLRKLGVDIASNHHINLIS
                     FVIILLYALGSIIAVYCISHFKRAE"
     gene            1768..2859
                     /locus_tag="O0V33_002181"
     CDS             1768..2859
                     /locus_tag="O0V33_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCX8976798.1"
                     /translation="MKFLKDTSIAEISSILYLIFPIAGIFFNEVYGPTWLYIISVIVF
                     SLSYLILVIVNNRLNTLMFYILLIIHYFIICYFVFSVHPMLSLFFFYSAFAIPFTFKN
                     NVKKMATNLFILTMIICFTITYLVHNDYFVAMTIYYVVILLIVFDNLKKVKDREYKKE
                     IEEKNRYINTLITEQERHRIGQDLHDTLGHVFASLSLKSELAYKIIDSDTENAKAELL
                     AINKLSRESLNKVREIIDDIRLPSFIEEIESICKILKDADIQFTFENKELAQLLSPTK
                     QSILVMIAREAINNVIKHANASKVHGQLRAMNEHKLQFIIQDNGEGIDSGCEIKSISQ
                     RVKHLNGTLEVESNKGTKLIIEMPTGGIA"
     gene            2856..3458
                     /locus_tag="O0V33_002182"
     CDS             2856..3458
                     /locus_tag="O0V33_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCX8976799.1"
                     /translation="MISLIIAEDQNMLRQAMIQLIKLHGDFEILADVDNGLDAIKIIE
                     AHHPDVVILDIEMSGMTGLEVLSEIRKKHLNIKVIIVTTFKRPGYFEKAVANDVDAYV
                     LKERSIEELVETIYKVYNGKKEYSASLMTSFFTDKNPLTPKEQIVLREIGNGLSSKEI
                     SEKLFLTDGTVRNYTSVRIDKLFADNRFDAWKKANEKGWI"
     gene            complement(3571..3759)
                     /locus_tag="O0V33_002183"
     CDS             complement(3571..3759)
                     /locus_tag="O0V33_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HCX8976800.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
     gene            3898..4431
                     /locus_tag="O0V33_002184"
     CDS             3898..4431
                     /locus_tag="O0V33_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCX8976801.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHKGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLNIWSK"
     gene            complement(4574..5428)
                     /locus_tag="O0V33_002185"
     CDS             complement(4574..5428)
                     /locus_tag="O0V33_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976802.1"
                     /translation="MSFYVVLIIIIVALIGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEMSNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     NQITAIFK"
     gene            5759..6334
                     /locus_tag="O0V33_002186"
     CDS             5759..6334
                     /locus_tag="O0V33_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976803.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDNTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
     gene            complement(6388..7770)
                     /locus_tag="O0V33_002187"
     CDS             complement(6388..7770)
                     /locus_tag="O0V33_002187"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCX8976804.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
                     DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENF
                     TDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGE
                     GMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHG
                     VALKNLCDKN"
BASE COUNT         2835 a         1038 c         1210 g         2777 t
ORIGIN      
        1 cgattataga ggctcttttt attttatcca aattaaataa cgataaatat gttatgtaaa
       61 cctaatgatg acatttgtca tttcaaatct atgacaaagt atcctatttt aaatcgaata
      121 aatttactaa tcttaaatta ataaaatatt aagaggtggt tcatttgatt caaatatcta
      181 atattaataa atcatttaag aaaaatcgtg ttttaaaaaa catttcattt gatattgaaa
      241 aaggtacatg taccgcatta atcggaaaaa atggtgctgg aaaatcaaca ttagttgata
      301 ttttaagcaa taaaataatt gctgatgatg gcgtgatttt ggataaagac aaattattgc
      361 aaagtgaaaa tcgcagtata atgtttcaga aaacgatgtt tccagatcaa ttgaaggtaa
      421 ttggaattat caaattatat caatcatttt atgaaaatcc attaacattg gacgtaataa
      481 tagaactaac aaagtttaat tctaatcaat tgaatcaatt tgcaaataaa ttatctggag
      541 gtcaacaacg attacttgat tttgtaatat ctttaatagg acaaccacag cttattttgt
      601 tagatgaacc aacgtcaaat atggatattg aaatgagaga atatttttgg tcaataatag
      661 ctaagttaaa agaagaaaat cggacgattc tttatacatc gcactatata gaagaagtag
      721 agcgcatgtc cgacaaaatt attttgatag aaaatggaga aatcaaactt aatgattcaa
      781 cgtcacatat tagaaccaat cagcaatctc agattacgtt atccgatgaa tataaaagaa
      841 agttaaaacc agataaagat gatttagtta ttcaaaaaaa tcataatggc actatcaaaa
      901 ttattacttc aaatgtaaat gatacgattt tatatcttca acaacttcat attaatttgg
      961 atgatattga aatacaaaaa gtctcaattg ttgattcata cttcaacaat aaaaagcaaa
     1021 ggggatctaa ttatgatact aagttacttg aaaatcgaat ttaaagttat actgcgtaaa
     1081 aaaacaacat taatattatc tattttattt cctgttattt tctatatatt atttacttcg
     1141 atattggatt tgccggaaga tgttaaacct aaattttata aagagtatat gtatagtatg
     1201 acggtttata gtttgttaag ttttagctta ctaacttttc cattagatat tattaatgaa
     1261 aaacaaaatg gatggcgcca aagattaatg gtaacaccat ttacttttac tagttattat
     1321 atttcaaaag tagtgaaaac tatgctgcaa tttgcaatag cgatattagt gatttttatg
     1381 gttgggcatt tttataaagg tgttgaaatg agtgcagttc aatggttaga gtcaggaata
     1441 tttttatggt taggtgcgtc tctattaata acttttggca tattattttc tttgttaaat
     1501 gatattcaaa aaacaagtgc tttagccaat atcgtaacaa ttggattagc agtattaggt
     1561 ggattgtggt ttccgataaa cacatttccg aattggcttc aacatgttgc tcatgtttta
     1621 ccgagctatc atttgcgtaa actaggtgta gatattgctt caaatcatca tatcaattta
     1681 atatcatttg ttataatact cttgtatgct ttagggagta ttatagcagt atattgtatt
     1741 agtcatttta aaagggcgga ataaaatatg aaatttttaa aagatacttc aattgctgaa
     1801 atatcgtcta tactttatct aatttttcct attgccggta tattttttaa tgaagtatat
     1861 ggtcccacat ggttgtatat tatatcagtc attgtctttt cgttatcgta tcttatatta
     1921 gttatagtaa ataatagact taatacatta atgttttaca ttttgttgat tattcattat
     1981 tttattattt gttattttgt tttcagtgta catccaatgc taagtttgtt tttcttttat
     2041 agtgcttttg ccattccatt tacttttaaa aataatgtta aaaaaatggc aactaatctt
     2101 ttcatattaa cgatgattat atgttttaca attacttact tagtacataa cgactatttt
     2161 gttgcaatga cgatttatta tgttgttatt ttgttaatcg tgtttgataa tttaaaaaaa
     2221 gtgaaagatc gtgaatataa aaaagaaata gaagaaaaaa atcggtatat taatacgtta
     2281 ataactgaac aagagcgtca tagaataggt caagatttac atgatacgct tggtcatgtg
     2341 tttgcaagtt tatcattaaa atcagaatta gcttataaaa ttattgattc tgatactgaa
     2401 aatgcaaaag ctgaattgtt agcgattaat aagttgtcgc gggaatcatt gaacaaagta
     2461 cgcgaaatca tcgatgatat aagattacca tcatttattg aagaaattga gagtatctgt
     2521 aaaattttaa aagatgctga cattcagttt acgtttgaaa ataaagaact ggcacaatta
     2581 ttgagtccta caaaacaatc cattttagta atgattgctc gtgaagcgat aaacaatgtg
     2641 attaagcatg caaatgcttc aaaagttcat ggtcaattac gagctatgaa tgaacataaa
     2701 ttacagttca ttatacagga taatggagaa ggtattgaca gcggctgtga aataaagagt
     2761 atttcacaac gagtaaaaca tttaaatgga acattagaag ttgaatcgaa taaaggtact
     2821 aaattaatca tcgaaatgcc tacaggggga atagcatgat ttctttaatt attgcagaag
     2881 atcaaaatat gttacgacag gcaatgattc aattaattaa actacatggt gattttgaaa
     2941 ttttagccga tgtagataat ggtctcgatg caataaaaat tattgaggca caccatcctg
     3001 atgtagttat tttagatatt gaaatgtcag gcatgactgg acttgaagtt ttatcagaaa
     3061 ttagaaagaa gcatttgaat attaaagtga ttattgtaac aacttttaaa aggccggggt
     3121 attttgaaaa agctgttgca aatgatgtgg atgcatatgt tttaaaagaa cgttctatag
     3181 aagaattagt ggaaacaatt tataaagtgt ataacggtaa gaaagaatat agtgcttctc
     3241 ttatgacttc atttttcaca gataaaaacc cattaacgcc aaaagaacaa attgtattaa
     3301 gggaaattgg caatggttta agtagtaaag aaataagtga aaaattattt ttgacagatg
     3361 gaacagttag aaattataca tctgttagaa ttgataaatt atttgcagat aatcgttttg
     3421 atgcttggaa aaaggcaaat gaaaaaggct ggatctaaat acaaacaaaa aagtattgag
     3481 tgttttgatt taagagactt tgaaagtgag tcaataatca aaatcttact caatactttt
     3541 attttggtac tcgcaagtta gatttgttaa ctaatttttt cgtttattat aaaacgaaac
     3601 aatcaggtaa acgataaagc ctacaaagat acccaataaa atagatagta ctgccgtcac
     3661 tattaatggt aatttaaaaa atatttgtag gaaaatacca atgataattg cgataattac
     3721 tgcaattaat gtgactgtat tttcatttga aatgttcatt cattttcact ccttaacaat
     3781 aacattatat catgctatag ctttccaaaa tattgaaata tgtagatatg gctattgacg
     3841 atatttctta acttttatat gattaatcgg aatgaaaaaa gagaagtagg tggcaaaatg
     3901 aagtcaaata aatcgcttgc tatgattgtg gtagccatca ttattgtagg tgtattagca
     3961 tttcaattta tgaatcataa gggtcctttt aaaaagggaa caaatcatga aactgtacaa
     4021 gatttaaatg gtaaagataa agtacatgtt caaagagttg tggatggtga tacatttatt
     4081 gcaaatcaaa atggtaaaga aattaaagtt aggcttatag gggttgatac gccagaaacg
     4141 gtgaaaccga atacgcctgt acaaccattt ggcaaagaag catcaaatta tagtaagaag
     4201 acattaacaa atcaagatgt ttatttagaa tatgataaag aaaaacaaga tcgctatggt
     4261 agaacattgg cgtatgtatg gataagtaaa gatcgtatgt acaataagga attagtggaa
     4321 aagggacttg ctagagaaaa gtatttttca ccaaatggca aatatagaaa tgtatttata
     4381 gaagcacaaa ataaagctaa acaacagaaa ttaaatattt ggagtaaata attaaatagg
     4441 tgattatata tattaaagta aaatatactt gaagcatcga tttattatta ccataaattt
     4501 atatatagaa ataatttaaa ggcataataa ccatagtgcg tatcgaacgt aatggattat
     4561 tatgcctttg attttacttg aatatagctg tgatttgatt aacctgttct ttatttaaag
     4621 ggtgtttaga tagctctatg gcttcgctaa taatacgtcg taacgaagat tttgttgaaa
     4681 ctgtatcaac atatttatct ctttcttcag agccttgaat gatattaact gtaccatcac
     4741 taaagtaaat aacaccggta ctttggactt taaagttaaa gttttgttca atatgatcct
     4801 ttaatgcttt agcattattt gctgctaatt gatacggatc ataatcataa aaatcataaa
     4861 taacacgatt tggttgaaca gtttctgtaa aagtatagat agactttctt gatgaattaa
     4921 actgatcatg atattgctga ctaatgtaat gaccgacaac tttttcaata ttactattgc
     4981 tcatttctgt atcatgctca tcaggtacat caaagtgata aaatgttttt tcaccccaac
     5041 tttttacatc aacgtttatt aaaccgatat ctgaaacaat gataaaatca aatgaacgtg
     5101 catactcgaa aaacggatgt ttagtagcta aattgcttgt aacgataata tcgtaatatt
     5161 ttaatttacc attttcgaga tagctgtcta aaatcttgcg taattctctt ttagcatttt
     5221 tgtatgcatg gtggccaaca tcattattag aactcagcat ttgacttctt aattcagcat
     5281 tttctgcact taatgctttg ttctttttaa taagttgctt tcttgcataa acttcggtat
     5341 ctattttact attactatac ctttgattta aaactaatat accaattaat gctacaatga
     5401 taatgataag tacaacataa aaagacattt tttcaccaat cctttttgac ttctttaact
     5461 ttgtatacaa taataattaa taaagattaa ttgttattca atttcccaca tttttattag
     5521 ttgattttag ttcatcattg ttataatcaa attataaact gacagatatt gatgttcaat
     5581 gaatatgacg tgaaagattc gtgaattcaa gtttatgtcg aatttatgtt ataacggtca
     5641 tttaaattac agaattaggt cactcatagt attttgaaga ttgaattcat taattttaaa
     5701 atgtataatg atatttgtga aagcgcttgc ttaggaggtg tatttgagag tgaatgaaat
     5761 gaatgctaaa gaacaattag tggacaattt aatgaaaaca tcatcgcaat tatttaaatt
     5821 tcacggtgaa gttgccatgc agcttttctt aaatgatgaa ttaaaattac cttctattgt
     5881 tgaaatatgc gtggaacgta agcgtttaag tgatattgtg aaagttattc cgcaatcata
     5941 tgcgttacta tacatagata agcaagatca agcaatagct aaagaagatt tatcactttc
     6001 aaaaattgca aaagtttatg tgcaatatga taatacaaca ataatgagta ttttcgttta
     6061 tgatgttgta aacgatgaat ggatttttag attggatccg aatatacgta tacctaagag
     6121 taacatttac ttccatagtc taaattggga tgtggattat attaaaccag agattgttct
     6181 aatgtatgat ctaatgcaac accatcagta tcatcattat tccaattata aacgcgtcat
     6241 agatgcatta agctactatc aattttttat tttaaaattt gtagtaggtg agcaacgtat
     6301 taaggatgca atccagagaa caataaataa ttaagaaaaa gcaattcata acgcagttga
     6361 atacatgtgt tacgaattgc ttttatatta gtttttatca cacaagtttt ttaatgcaac
     6421 cccgtgatag caaaactcat atgtagataa tacagctttt tcagcatcat ctacatgaat
     6481 tccaaacatc atcgaaattt ccgaagcacc ttggttaatc atttttaagt taatttttga
     6541 ttcagctaag gcatgtgtaa ttttatttgc agtaccaatg actttattca taccttcgcc
     6601 gacaatcatt aaaattgcta aatcatgctc aatacttagc tcatcaacat cacatttttg
     6661 acgaatttca tttaatactt ttgtttcttt attttgaatt tgttttgaac gcataacgat
     6721 actgatagta tcaatacctg aaggcatatg atcaaatgaa atattattat cctctaagac
     6781 acctaatatc tttctagtaa agccgacttg tctattcatt aaatactttt tgatattaat
     6841 aacagtaaaa tctttatcac aacttatacc gctaatcaca tttttcgcat ttatttctct
     6901 atcatgcact ataaatgtac ctttatcttg agggcggttc gtatttttaa taactacagg
     6961 gatgcgatct ttataaagtg gttgtaaggc ttcatcatgg aaaacactaa aaccagcata
     7021 agataattca cgcatttctc gataagtgat ttcttcgatt aattcaggat ctttgatgat
     7081 atttggatta gctttataaa taccagacac atcggtgaaa ttttcataaa ttgtagctct
     7141 aacaccactt gatatgatgg cgccagttat atctgatccg ccacgtggaa atgtaactat
     7201 atatccttca tgagatacgc caaaaaatcc tgggataatt agtttttcat cataatctct
     7261 taatttttta atttcagagt aagcactatc taatatttgt gcttcttgtg ggacgtcagt
     7321 aacaaaaata cccgcttcct tcggtgatat atattttgtt ggtatacctt gactattatt
     7381 atataaagct atcaattgcg cattaaaatc ttcaccacaa gaaagtaatg catctaatag
     7441 tctcttcggt tcatttttta attgattaat ataatgttcc aaagtcacat ctatcgtccg
     7501 taaaatactt tcatccattt gcaattcttt tacaatatca tcataacgct gaataatttc
     7561 tcttttttta tcatgataat caagatgatt aatgaccttt tcatataatc tgattaacaa
     7621 atcagttgtt ttaatatcat tatcatgtct tttacctgga gcagaaacga taacaatctt
     7681 tcgctctgga tcagaattaa caatatttaa aaccttttta atttgagtag cattggagac
     7741 ggagctacca ccgaatttgg aaactttcat agtgcatgcc aacctttcta aaaaatcaga
     7801 aaattgtatt cagttttgta agtaagaatg atattatgta tttatatttt gaatattacg
//
LOCUS       DAKSQE010000109         7845 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.109, whole genome shotgun sequence.
ACCESSION   DAKSQE010000109 DAKSQE010000000
VERSION     DAKSQE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7845
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.109"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            290..1210
                     /gene="mvk"
                     /locus_tag="O0V33_002188"
     CDS             290..1210
                     /gene="mvk"
                     /locus_tag="O0V33_002188"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCX8976805.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHKFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            1215..2198
                     /gene="mvaD"
                     /locus_tag="O0V33_002189"
     CDS             1215..2198
                     /gene="mvaD"
                     /locus_tag="O0V33_002189"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCX8976806.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDLQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYNDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            2211..3287
                     /locus_tag="O0V33_002190"
     CDS             2211..3287
                     /locus_tag="O0V33_002190"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCX8976807.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEEADQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            3464..3805
                     /locus_tag="O0V33_002191"
     CDS             3464..3805
                     /locus_tag="O0V33_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HCX8976808.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFAFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKEEQDRKL
                     KEKLEKRKAQQ"
     gene            complement(3857..5179)
                     /locus_tag="O0V33_002192"
     CDS             complement(3857..5179)
                     /locus_tag="O0V33_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCX8976809.1"
                     /translation="MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSSKMYGGTCI
                     NVGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDSNIDVLDFKAQFK
                     SNTEVNLLDQHGDIVDSITAPHIIINTGATSIIPNIKGLDQAKHVFDSTGLLNISYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAHAITDLENKGIAL
                     HTNVETTELSSDNYHTTVHTNVGNFEADAVLLAIGRKPNTDLALENTDIELGDRGEIK
                     VNAHLQTTVPHIYAAGDVKGGLQFTYISLDDYRIIKSSLYGNQSRTTDNRGSVPYTVF
                     IDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMILG
                     ATLYGKQSEELINIIKLAIDQNIPYTILRDNIYTHPTMAESFNDLFNF"
     gene            complement(5166..5606)
                     /locus_tag="O0V33_002193"
     CDS             complement(5166..5606)
                     /locus_tag="O0V33_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCX8976810.1"
                     /translation="MNLEFNIAVHVLTFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMINTIRGKDGGYLANDRSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYQQDEQNNIINFYKEKTIKDVIEDIQKEDLCHENI"
     gene            5664..5765
                     /locus_tag="O0V33_002194"
     CDS             5664..5765
                     /locus_tag="O0V33_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976811.1"
                     /translation="MCQYLCLKSVELGVIIMMIYYLKVVALTVRLRN"
     gene            6230..7624
                     /locus_tag="O0V33_002195"
     CDS             6230..7624
                     /locus_tag="O0V33_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCX8976812.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDAL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIRTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEQKAEKETLNKADFAVIKAYAKEHPEDDIPKSIMKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDMANDDKTVGEALSHNALTTGIGIGAGAGMTATIALFASNPVGWAV
                     IGGFVFGSVSTMLVDWAYQNNKAGIKDKADWVGHKLDDGIDVVKKSTEKAVDSVGEAT
                     KSISNHLNPMKWSW"
     gene            7625..>7845
                     /locus_tag="O0V33_002196"
     CDS             7625..>7845
                     /locus_tag="O0V33_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCX8976813.1"
                     /translation="MLLCESKVINKNPKYRVIKYGDEYLMIDLVSTWLTLFLPMINWL
                     IPKKYVKISKKEFDDLNIVKPVKNKAFWPV"
BASE COUNT         2782 a         1104 c         1540 g         2419 t
ORIGIN      
        1 gttagggtgg aaaattataa attgtagggc tattttataa aaagataaag aatctgaaat
       61 atcactcgat aagatttaaa ctttgaagta aacgaaagtt agttttatac taatcaagtt
      121 aagtcatata taaatttaaa ataaagttta tacataatat gaataatgtg agataaagac
      181 tgcaatgaaa tttaacgcga atgcgtatgc aacttagaat atgatgtctt ttactaataa
      241 ataaaaacaa tgtatcaagt ataggataaa tataaaaggt gatattgaca tgacaagaaa
      301 aggatatggg gaatcgacag gtaagattat tttaatagga gaacatgctg ttacatttgg
      361 agagcctgct attgcagtac cgtttaacgc aggtaaaatc aaagttttaa tagaagcctt
      421 agagagcggg aactattcgt ctattaaaag cgatgtttac gatggtatgt tatatgatgc
      481 gcctgaccat cttaagtctt tggtgaaccg ttttgtagaa ttaaataata ttacagagcc
      541 gctagcagta acgatccaaa cgaatttacc accatcacgt gggttaggat cgagtgcagc
      601 tgtcgcggtt gcttttgttc gtgcgagtta tgatttttta gggaaatcat taacgaaaga
      661 agaactcatt gaaaaggcta attgggcaga gcaaattgca catggtaaac caagtggtat
      721 tgatacgcaa acgattgtat caggcaaacc agtttggttc caaaaaggtc atgctgaaac
      781 gttgaaaacg ttaagtttag acggctatat ggttgttatt gatactggtg tgaaaggttc
      841 aacaagacaa gcagtagaag atgttcataa actttgtgag gaccctcagt acatgtcaca
      901 tgtaaaacat atcggtaagt tagttttacg tgcgagtgat gtgattgaac atcataaatt
      961 tgaagcctta gcggatattt ttaatgaatg tcatgcggat ttaaaggcgt tgacagttag
     1021 tcatgataaa atagaacaat taatgaaaat tggtaaagaa aatggtgcga ttgctggaaa
     1081 acttactggt gctggtcgtg gtggaagtat gttattgctt gccaaagatt taccaacagc
     1141 gaaaaatatt gtaaaagctg tagaaaaagc tggtgcagca catacatgga ttgagaattt
     1201 aggaggttaa tgcgttgatt aaaagtggca aagcacgtgc acatacgaat attgcactta
     1261 ttaaatattg gggtaaaaaa gatgaagcac taatcattcc aatgaataat agcatatctg
     1321 ttacattaga aaaattttac actgaaacga aagtcacttt taacgaccag ttaacacagg
     1381 atcaattttg gttgaatggt gaaaaggtta gtggcaaaga attagagaaa atttcaaaat
     1441 atatggatat tgtcagaaat agagctggca tcgattggta tgcagaaatt gaaagcgaca
     1501 attttgtacc aacagcagca ggattggctt catcagcaag cgcatatgca gctttagcag
     1561 cagcttgtaa tcaagcgcta gacttgcagc tgtcagataa ggatttatcg agattggcgc
     1621 gaatcggttc gggttctgcg tcgcgtagta tttatggtgg atttgcagaa tgggaaaaag
     1681 ggtataatga tgagacgtca tatgccgttc cacttgaatc gaatcatttt gaagatgacc
     1741 ttgccatgat atttgttgtg attaatcaac attctaaaaa ggtacctagt cgatatggta
     1801 tgtcgttgac acgaaacaca tcaagatttt atcaatattg gttagatcat attgatgaag
     1861 atttagctga agcaaaagca gcgattcaag acaaagattt taaacgcctt ggtgaagtaa
     1921 ttgaagaaaa tggtttgcgt atgcatgcca cgaatctagg atcaacaccg ccgttcacat
     1981 atcttgtgca agaaagttat gatgtaatgg cgctcgttca cgaatgccga gaagcggggt
     2041 atccgtgtta ttttacaatg gatgcgggac ctaatgtgaa aatacttgta gaaaagaaaa
     2101 acaagcaaca gattatagat aaattattaa cacagtttga taataaccaa attattgata
     2161 gtgacattat tgccacagga attgaaataa ttgagtaagg aagagataaa atgattcagg
     2221 tcaaagcacc cggaaaactt tatattgctg gagaatatgc tgtaacagaa ccaggatata
     2281 aatctgtact tattgcgtta gatcgttttg taactgctac tattgaagaa gcagaccaat
     2341 ataaaggtac cattcattca aaagcattac atcataaccc agttacattt agtagagatg
     2401 aagatagtat tgtcatttca gatccacatg cagcaaaaca attaaattat gtggtcacag
     2461 ctattgaaat atttgaacag tatgcgaaaa gctgcgatat agcgatgaag cattttcatc
     2521 tgactattga tagtaattta gatgattcaa atggtcataa atatggatta ggttcaagtg
     2581 cagcagtact tgtatcagtt ataaaagtat taaatgaatt ttatgatatg aagttatcta
     2641 atttatacat ttataaacta gcagtgattg caaatatgaa gttacaaagt ttaagttcat
     2701 gcggagatat tgctgtgagt gtatatagtg gatggctagc gtatagtact tttgatcatg
     2761 aatgggttaa gcatcaaatt gaagatacta cggttgaaga agttttaatc aaaaactggc
     2821 ctggattgca catcgaacca ttacaagcac ctgaaaatat ggaagtactt atcggttgga
     2881 ctggctcacc ggcgtcatca ccacactttg ttagcgaagt gaaacgtttg aaatcagatc
     2941 cttcatttta cggtgacttc ttagaagatt cacatcgttg tgttgaaaaa cttattcatg
     3001 cttttaaaac aaataacatt aaaggtgtgc aaaagatggt gcgtcagaat cgtacaatta
     3061 ttcaacgtat ggataaagaa gctacagttg atatagaaac tgaaaagcta aaatatttgt
     3121 gtgatattgc tgaaaagtat cacggcgcat ctaaaacatc aggcgctggt ggtggagact
     3181 gtggtattac aattatcaat aaagatgtag ataaagaaaa aatttatgat gaatggacaa
     3241 aacatggtat taaaccatta aaatttaata tttatcatgg gcaataaatg atttgatagg
     3301 ggagcaatca tctaatttaa ttagaagata aatgctccat tttttattgc cagctgtgtt
     3361 acgtatcttg aaaaaaatat taaaaagagt aaaatagata gagttaacga aaaaattaat
     3421 gaaatcgacg atatagatat gataaagaaa ggtgggtagc aatatgaaaa atacattcct
     3481 tatttgtgat gaatgtcagg cagtcaatat aagaacgtta caaaagaagt tggaaaaatt
     3541 agatcccgat gctgaaatcg tgataggttg tcaatcttat tgtggacctg gacgccgaaa
     3601 aacattcgct tttgttaata accgcccact ggctgcgctt actgaagaag aattaatcga
     3661 aaaagtttct caacaattaa agaaaccacg tgatcctgaa gaagaagagc gtttaagaaa
     3721 acgacatgaa gaacgtaaac gtcgtaaaga agaacaagat agaaagctta aagaaaaatt
     3781 agaaaagcga aaagcacaac aataaagcct gatggcagca tcattcaatg cgtgccacca
     3841 ggtttttatg ttttgtctag aaattaaata aatcattaaa tgattcggcc atcgtaggat
     3901 gcgtataaat attatctcgt aatatggtat atggaatgtt ttgatcaatc gcaagtttaa
     3961 ttatattaat taattcttca gattgcttac catataatgt agcacctaaa atcatattat
     4021 tttcattatt aatgactact ttaaataaac ctcttggatc attgttaatt ttgtgacgag
     4081 gtatagcact tactaaaagt tgatgctcag tgtaatcata atgttgagcg gcagcttctt
     4141 tgctagttaa tccaacacgt gataatggtg gatctataaa tactgtataa ggcacgctgc
     4201 ctctattgtc agtcgtacgt gactgattac catataaaga tgatttgata attcgataat
     4261 catctaaaga tatatacgta aattgaagtc cgcctttaac atcacctgca gcataaatat
     4321 gcggcacagt tgtttgaaga tgagcattga ctttaatttc gcctctgtcg cctaattcga
     4381 tatcagtatt ttctaatgct aaatccgtat tcggtttgcg tccgatagcc aaaagtactg
     4441 catcagcctc aaagttacca acgttagtat ggacagttgt atgataattg tcagatgaca
     4501 attcagtcgt ttcaacattt gtatgcaatg caatgccttt attttctaag tcagtaatag
     4561 catgtgcaac gacatcttga tcttcgcgtg gcataaatga ttcaccacgt tctaatactg
     4621 taaccttact acctaaattc gcaaacattg aagcaaattc taaggcgata taaccgccac
     4681 ctacaataac gaggtgctta ggttgatagc taatgtttaa taaacctgtt gaatcgaaga
     4741 cgtgtttagc ttgatcaagg ccttttatgt taggaatgat agaggtagca ccggtattaa
     4801 taatgatatg aggtgcagta atactatcga cgatatcacc atgctgatct aataaattca
     4861 cttcagtatt agatttaaac tgcgctttaa aatccaatac atcaatgtta ctgtcgtctg
     4921 ctaataagtg gtaattttta ttgtttagcg cattgacaac atcgttttta cggttataac
     4981 ttgcttcaaa ggatttgcct tctaatccat catgtacaag tgtcttcgaa ggtatacatc
     5041 ctacgttgat acaagtgccg ccatacattt tcgaagattg ttcgataact gcgacgtgtt
     5101 gacctgttga tgcagcgtat ttcgctaaag ttttaccagc tttcccaaat cctattacaa
     5161 ttaaatcata tgttttcatg acataaatcc tccttttgaa tgtcttcaat gacatctttg
     5221 attgtttttt ctttataaaa attaataatg ttattctgtt cgtcttgttg ataatgtgac
     5281 atggtagttg caatattacg agcaatttga cagtgactac cttcgtcgcc agtaaatagt
     5341 cgtgtgtgat gttctttctc taagacaaaa tgtttatata atgttgctaa tgagacatca
     5401 gcgctacgat catttgctaa ataaccacca tctttacccc gtattgtgtt aatcattttt
     5461 aaatcgacaa gttgagtcgt cacgcggcgt aattgaacag gatttaaaca agttaattca
     5521 gctaatgaac tactattgaa tttttctgaa tgatgcttag ttaaaaaagt taatacatgt
     5581 acggcaatgt taaattctaa attcaatggt tctcacctcg taattgtaac tgtattagtt
     5641 acaattaaaa atcattttgc tcaatgtgtc aatacttatg tttgaaaagt gttgaattag
     5701 gtgtgattat tatgatgatt tattatttga aagttgttgc attaacggtg agattgcgta
     5761 attgaatttt agttatgtat gaactaatag atatgaaatg agtattgaaa ataagggcaa
     5821 taaaattttg aatgatgatg ttgtgtgatt gtataaactt gttcgtattt tagagacgtt
     5881 ttgatatttc gcataatgag gaaatacttt taacaactga tgacatattt taatagtgaa
     5941 tatattttga gatattaata tatttaatgt taaggatgtt gtcgcaataa aattaaaata
     6001 ttgaaagata atttttattt attaattgcg aatatacaaa gacaccatta tatccatatg
     6061 ctaaaataag aaaaaaggcg acatgttatc tcaatttacc tgcatctaga ctcctttaca
     6121 taagaaataa tcatcgccga gttttaaaat tttacaattt tgtagatgta aaaagcattt
     6181 gattttaaat agtttgaaaa tcatatattt ttgaaaaagg agcatgccga tgagtattga
     6241 catgtattta gacagatctc gaaaccaagc ttcaagtgtg ggtaatttga gtcaaacaat
     6301 gaattcaaat tatgatgcgt tggaaaaagc aattactcaa tttatcaatg atgatgcgct
     6361 taaaggtaaa gcgtatacgt cagctaagca attttttagt acggtgttaa ttccattatc
     6421 aacaagtatg aaaacattga gtgatttaac gaagcaagct tgcgataatt ttgtgtcacg
     6481 ttatacgagt gaggttgata gcatatcttt aaaagaatca gagcttgaag aagatatcag
     6541 atcattaagt caacaaatta cgcgatatga aaatttgaat aacaatttga aaaagcatgc
     6601 ttccgataat cagcaagcca tttcatcgaa ccaacaaata atacgaacat taggtcaaca
     6661 aaaacatgaa ttagaagaga agctacgcaa attgcgtgag tttaatcaaa aatcaccaga
     6721 aatatttaaa gaagttgaag aatttcaaaa aattgtacaa caaggactta cccaagcgca
     6781 aaatttttgg aacttttcaa caaatcaatt taatatacct tcaggtaaag aacttgattg
     6841 ggctaaagca agtcatgaaa aatatttgaa agttgctatg gggaaaattg aacaaaaagc
     6901 agagaaagaa actttaaata aagcagactt tgctgttata aaggcatatg ctaaagaaca
     6961 tccagaagac gatatcccga aaagtataat gaaatatata aatgacaata aagatagtat
     7021 taaacgagat ataggattag atattacgtc aacactttta gagcaaggcg gtataaatgc
     7081 aagtaaattc ggtgtattta ttaatacagc aggtggagtg aaaggcccag ccggtccaaa
     7141 ttcatttgtg gaagtcaaac gtacatcagg taatgtgttt atagaaaatg gtagtaaatt
     7201 tgcaaaaggc ggaaaatacc taggcaaagg tgttgcaggt gtaggatttg gtataggtat
     7261 gtatgatgat atggcaaacg atgataaaac agttggagag gcattgtcgc ataatgcttt
     7321 aactacagga ataggtattg gcgcaggtgc gggtatgact gctactatcg cgttatttgc
     7381 aagtaatcca gtaggatggg ctgttatagg tggctttgtg tttggttcgg tttcgacaat
     7441 gcttgtagat tgggcttatc aaaataataa ggctggtata aaagataaag cagactgggt
     7501 aggacataaa ctagacgatg gtatcgatgt tgttaaaaaa tctacagaaa aagccgtaga
     7561 tagtgttggt gaagctacaa aaagtatttc taatcattta aaccctatga aatggagttg
     7621 gtaaatgttg ctatgtgaat ctaaagttat caacaaaaat cctaagtata gagttattaa
     7681 atatggtgat gaatatttaa tgattgattt agtaagtacc tggttaactt tatttcttcc
     7741 tatgattaat tggttaattc caaaaaaata tgtcaaaatc agtaaaaaag aatttgacga
     7801 tttaaacatt gtcaaacctg ttaaaaataa agctttttgg ccagt
//
LOCUS       DAKSQE010000110         7715 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.110, whole genome shotgun sequence.
ACCESSION   DAKSQE010000110 DAKSQE010000000
VERSION     DAKSQE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.110"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(169..861)
                     /locus_tag="O0V33_002197"
     CDS             complement(169..861)
                     /locus_tag="O0V33_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCX8976814.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFEYAGVTKEACIEAPGYRVKESPVHFECEYVQTIRIPTGDPVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPSASKEELAGLE
                     GRNFDNQSDNKK"
     gene            1175..2119
                     /locus_tag="O0V33_002198"
     CDS             1175..2119
                     /locus_tag="O0V33_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976815.1"
                     /translation="MRKIMRALLCTSIIVSGCSFIPKYQTKDIPKTVSVKDYDGQYIG
                     EHKKRNEVFLKKHKDEAIKKYKNYVKDTFGYDCKINLVEAYTNKSGFSEKSKVDGISV
                     VGTVNYDVPFQLQLLFVESGNGIIISTFTPGHINETSAAVSAMMYKRYEHEIEQARLK
                     LKCEIEKDSYYAMSEKLQVKQEFNGVTRQYLNFRTDSIDDLDKFKKEFKPVMHLKGDA
                     FNQQLQSLINKYPQIQKNMKSEFIAYYDKGENRETVANYVWNLQKTTNDTMKLYPGNK
                     SIIFYKDKVSASQLDEHKRLQSDSQEISISGGENDEKK"
     gene            2106..2408
                     /locus_tag="O0V33_002199"
     CDS             2106..2408
                     /locus_tag="O0V33_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976816.1"
                     /translation="MKKNDHITDLDAIELSSAWNYNSKLVTGKNALLSDSLLRDIKIL
                     SSNAKGDADMLETELHAVINAMSQNEGKSVDALKNSTASLKNNLTKLKEQLDKTTH"
     gene            2558..2917
                     /locus_tag="O0V33_002200"
     CDS             2558..2917
                     /locus_tag="O0V33_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976817.1"
                     /translation="MILRYVTNLKLAKELYQASKPKLQEDRGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSVAAYKHFEAGQGKKGAQHALDL
                     LGLATLSLLDTFSCNKK"
     gene            complement(3002..3613)
                     /locus_tag="O0V33_002201"
     CDS             complement(3002..3613)
                     /locus_tag="O0V33_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCX8976818.1"
                     /translation="MHTFDAQSLTARENDKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMIAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTELSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGDFKINPHALQAVSRLAGNDYAKLGGLFTIDRPEK"
     gene            complement(3692..4168)
                     /locus_tag="O0V33_002202"
     CDS             complement(3692..4168)
                     /locus_tag="O0V33_002202"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HCX8976819.1"
                     /translation="MAVVYVAGGCLWGVEAFFTTIPGIIHTEAGRANGRSSKLDSPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNRQGNDIGEKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDKIAVEVLPLSNYIKSAEEHQQHLEKYPEDIHMCHISIDLLNKYK"
     gene            complement(4171..4671)
                     /locus_tag="O0V33_002203"
     CDS             complement(4171..4671)
                     /locus_tag="O0V33_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCX8976820.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATPEALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVPTNNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFENLAKNQHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            complement(4906..5673)
                     /locus_tag="O0V33_002204"
     CDS             complement(4906..5673)
                     /locus_tag="O0V33_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HCX8976821.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILQGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            complement(5670..6362)
                     /locus_tag="O0V33_002205"
     CDS             complement(5670..6362)
                     /locus_tag="O0V33_002205"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HCX8976822.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIQDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNKDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            complement(6379..7041)
                     /locus_tag="O0V33_002206"
     CDS             complement(6379..7041)
                     /locus_tag="O0V33_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HCX8976823.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNRISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILLIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
BASE COUNT         2576 a         1373 c         1094 g         2672 t
ORIGIN      
        1 atatcaatac attttcaata aacctagtgt tatatatctg aatttctaaa aaattcttct
       61 ttaaaattat aaatatagcg acatatcgct tcctgatttt gaaatataaa aatacatcga
      121 tactaatttc aacgtgtagt ttcgatgtat taaaaatgat tgaattcatt atttcttgtt
      181 atctgattga ttatcaaaat ttcttccttc taaacctgca agctcttctt tagaagctga
      241 tggtgctttc atttcaaata tttcattcac aaccgtgtaa tcataatatc ctaacctagc
      301 aataggttta atcgacttaa tgtctaattt accattatca agaatgacct tatcgtcaat
      361 atgaacctga gcaactcttc ctataacaat atcaactgtt gatacagggt ctcctgtcgg
      421 aatacgaatc gtttgaacgt actcacattc aaaatgtact ggcgattctt tgactcgata
      481 acctggtgct tctatacatg cttctttcgt cacacctgca tattcaaatt catcctcttc
      541 aggtggtagt gccttcgacg ataaattaac agcttctctt aaatcatacg tggccatgtt
      601 ccatacaaac caccctgtct cttctgcatt ttttactgta tctttacgct catgatcacc
      661 aagaacagat tgatttgctg cgaacataac cataggcgga tcccaagtta agttttgata
      721 ctgactataa ggtgctaaat tatctttccc atctttcgat acagtagaga tccaccctat
      781 tggacgtggt actgtactac ttttaaatgg atcgtgtggt aagccatgac tccttacacc
      841 ttgcttcggc gaataattca ttctatcctc acctcttatt agtaattaca tttaaggtta
      901 cgctctcatg acataagcgt ctaatataaa taatcaattt atttataagt agaaactata
      961 tatgacatgg ttgcttataa tttgcgttct tgattcgaaa atttcagata aggatttatg
     1021 caattaatat ttaataatgt tttttgttaa tttaattaat attagttaaa ataatcaaga
     1081 ttatattata ataatattga ataacattat tttagttttt gtataatttg cctatcaact
     1141 gaaaaacagc taatacaaag gagctaatga agatatgaga aaaattatgc gagccttatt
     1201 atgcacatca atcatagtaa gcggttgctc tttcatacca aagtaccaaa caaaagatat
     1261 tccaaagacg gttagcgtca aagattatga tggtcaatat attggtgaac ataaaaaaag
     1321 aaatgaagta tttctgaaaa aacataaaga tgaagcgatc aaaaaatata aaaactacgt
     1381 taaagacacc tttggttatg attgtaaaat taatttagtt gaggcctata ctaataaatc
     1441 tggatttagc gagaaaagta aggtagatgg tatctcagtg gttggtactg tcaattatga
     1501 cgttcctttt caactccaat tactttttgt tgaatctgga aatggcataa taatttcaac
     1561 atttacacct ggtcatataa atgaaacatc agcggccgtt tcagccatga tgtacaaacg
     1621 gtatgaacat gaaattgaac aagctcgatt aaagcttaaa tgtgaaattg aaaaagatag
     1681 ctattatgcg atgagcgaaa aacttcaagt aaaacaagaa tttaacggcg ttacaaggca
     1741 atacttgaat ttcagaacag atagtatcga tgatctggat aagttcaaaa aagaattcaa
     1801 acctgttatg cacttaaaag gtgatgcatt caatcaacag ttacaatctt tgattaacaa
     1861 atatccacaa atacaaaaaa atatgaaatc agagttcatt gcttattatg ataaaggtga
     1921 aaacagagag actgttgcta attatgtttg gaacctacaa aaaacaacta atgacacaat
     1981 gaaactttat ccaggaaata aaagcatcat attttataaa gataaagttt ctgcttcaca
     2041 attagatgag cataaacgtt tacaatcaga ttctcaagaa atctccattt ctggagggga
     2101 aaatgatgaa aaaaaatgat catataaccg atttagacgc aattgaatta agcagtgctt
     2161 ggaattacaa ttcgaaattg gtaactggca aaaatgcact actatcagat agccttttaa
     2221 gagatattaa aatattaagt tctaatgcta aaggtgacgc cgacatgtta gaaactgaac
     2281 ttcatgctgt tataaacgca atgtctcaaa acgaaggtaa atctgttgat gcacttaaaa
     2341 atagtaccgc ttctctaaaa aacaatctaa ccaaattaaa agagcaactc gataaaacta
     2401 cacattaatt tcatacattt tgagtgaaca gaattatatt tccatagcaa acattcctga
     2461 actacacatg ttacataaga gattcatttt tgaagaaacg ggtaaaatga taaaacaacc
     2521 gtttttcaaa aaatcaaata ttgggaggaa taaaaatatg attttacgtt acgtgaccaa
     2581 tttaaaactt gcgaaagagc tataccaagc cagtaaacct aaattgcaag aagatagggg
     2641 tatgattgaa ctgtttgaac atacttttgg cttgcaaaaa gaacttgtaa aatacgtcgg
     2701 cattgcagaa gcaactactg ctgctttata ctcagctagc tttatcaata aaaacatttc
     2761 tcgccttgct tcgctatcaa caataggtat tttaagtgta gctgcttata aacatttcga
     2821 agctggccaa ggtaaaaaag gtgcacaaca tgcattagat ttattaggtc ttgctacttt
     2881 aagtttacta gatacatttt cttgtaataa aaaatagtct tcattttaga agcacatact
     2941 ttagcacctg tgtacttttg attcaatcca taaacggtac accgctatag catgtgtttt
     3001 gttatttttc aggtctatct attgtaaata atcctcctaa tttagcataa tcatttccag
     3061 ctaaacgaga aacagcctgt aatgcgtgtg gattgatttt aaaatcaccc tcataaatac
     3121 tgtcatcaat atgataagtt acaatttcgc ctattattaa gtcagcccca tctaatacat
     3181 ctccaagcaa tatcatttgc gatagtttac attcgaatct cattttcgcg tctttaattc
     3241 ctggcgtctt aatcgttgta gatgttaaaa gtgataattc tgtacggctc aactcactgt
     3301 caccatatgc taacggcgct gcagtctcat taatatcttg aacattatct tcgtctgtaa
     3361 tatgcacaac aaagtctcca gtccgctcta tatttaaggc agtatctttt ctcttacctc
     3421 ctgcacgttg aactgcaata gcaatcattg gcggatgatt attaactata ttaaaaaaac
     3481 taaatggtgc tgcatttact gatgcatctt gatttaatgt tgtaacaaaa gctataggtc
     3541 gtggaataat tgaaccaatt aataatttat cgttttctct agcagttaat gattgtgcat
     3601 caaacgtatg cataatacct acctcttttc ttagtatatc taggtatttc tccgatttcg
     3661 gttaatttaa acatctattc tcctctgaaa atcacttgta tttatttagc aaatctattg
     3721 aaatatgaca catatgtata tcttctggat atttttctaa atgttgctga tgttcttcag
     3781 cacttttaat gtagttagac agcggtaaga cttccactgc aattttatct ctgtctttac
     3841 gtcgttcaat gaactgacgc gcttcaatta agtggtcatc tacacaacta tataaacccg
     3901 ttcgatactt ttctccaata tcatttcctt gtcgattcac actgtatgga tcaataattt
     3961 caaataaata attcatgatg tctgtaattg ttaacatacg atcatcaaaa tgaagtttga
     4021 cacattcagc ataaccatca tacggactgt ctaatttaga gcttcttcca tttgctcttc
     4081 ctgcttctgt atgtataatt ccaggtattg ttgtgaaaaa tgcttcaaca ccccatagac
     4141 accctcctgc tacataaaca actgccatat ttacacctca tcatcctttt ttatattttt
     4201 aacaaggtta taccatttaa taccgccatg acatgattct gatacacctt cattacgata
     4261 cccatatttt tcataaaatg aaattaatga ttctcgacat gttaacgtta caccatgtcg
     4321 atgttgattc ttagcaagat tttcaaaata gtttagtaag cgacctgcaa taccctgacc
     4381 ttgataattt ggtgctacaa caagacctaa cacactaata taaccacctt cactattatt
     4441 tgtggggaca tttttaaata aatcatcgct aatgtaacgc tcttttatga caggaccgtt
     4501 gatatatcca atcacttcat cattcttttc agctacaata aaagtttctt gaatttgttc
     4561 aattctcaat ttcaatgcct ctggtgttgc agcttcttcc ggtgaaaaac caatattttc
     4621 aagtgcaata atttgatcta agtcctgcat gattactttt cttaatacca taaaaacact
     4681 tccttagccc tatatatcaa aagtaacacc ttcatctata tattcattat tttcaaacta
     4741 ccttcactat aaaaaacgag tctttttatt aaaagccatt cgccttaatc aattagacaa
     4801 gttgtattat acgaattagc aaataatcat gattataatt cattttaagc atacttaaaa
     4861 agactggtac atgtatacca gtctaattcg aaaaaatata tttagttaaa accatttcaa
     4921 aaacgacttc acttttcccg gcttagtcgg tctgtctaaa taaacaaaat tatcattgat
     4981 tactgaactg gcatttcgaa gtaatgcttc aatgtcattc gaatatttct tcaatttatg
     5041 attgtgaaat aattcttgca tcaaaaatgg tctttggtca catgaatgtg catctgaagc
     5101 tacaaaatga gccaaattac attctataaa ttgtaatgat aacttttgaa tgttttttcc
     5161 aaatccacca actaaagaac tcgatgttaa ttgactcaat gccccatttg caacaagttc
     5221 atataatatt tctggatttt tggcgatact tctatttctt tcaggatgtg caatgattgg
     5281 tatgtagcct ctcgattgta tttcaaaaaa caattgtttt gtataatgtg gtacttcgcc
     5341 cgttggaaat tcaattagca aatatttcga acgattaata ccttgaatac taccattatc
     5401 taagccttgc agaatcgaat ctgtaattct aatttcttgc ccgggaaata atttaatatc
     5461 caatgcttga acttctggat gcgttcttaa ctccgccaat ttcacaagca cttgttgaaa
     5521 tgtattatca tatctcggat gcaaatgatg aggtgtcgct acaatacttg ttacaccttc
     5581 atccttagct tgctttaata gtgcaatact cttttcaatt gttttaggac catcatctat
     5641 atcaactaat atatggttat gaatatcaat catgattcat cagtcccata atatgcatag
     5701 taactagcac ttttatcttt aggcattcta tttaagacta cacctaataa tttagcacct
     5761 gttgcttcga taagttcttt tcccttttta acttcatctt tattattatt ttccgaatta
     5821 actacgtaga caacattgcc ggtaaacttt gaaaataatt gcgcatctgt aactgtgttc
     5881 actggtggcg tatcgataat aacaaagtta taattcatca ataacgtgtc atataaattt
     5941 gcaaatgccc ttgatgtaat taactctgac ggattcggtg ggattggccc agacgtcaag
     6001 acgtctaaat cttgaatttc agttgagata atactgtctt gataagttga ccaatttagc
     6061 aataaacttg aaagtccttc attgtttggc aaattaaaaa tatagtgttg cgtaggctta
     6121 cgcatatccc cgtctacgat taatgtttta taacctgctt gtgcatatgc aactgctaaa
     6181 tttgctgcaa ttgtagactt acctgcgcct ggtgcctctg aagtgattac aatgctttga
     6241 actgcactgt caggatttgc aaacataata tttgatctta tgcctcgaaa tttctcgcta
     6301 ataggtgact ttggttgttc atggacaatt aaacttgatg tacttcttcg tgtattcgtc
     6361 atggtaattc ctcgtaaatt aaaatttttg tattgaacct aaaataggta atcctagttg
     6421 cgattcaaca tcttcttccg tcttaatacg cttatctaat agttctttta agaaaataag
     6481 caatattgct aaaacaatac caacaatgat actgatcact aagttgacag atactattgg
     6541 agatactttt acagcattat catgtgctga ggaaagtatt gtaacattat caacactcat
     6601 aattttaggc atgtcatgtg caaaaacttt agatattcta ttaacaattt tgtcagattc
     6661 agatttattc ccagttgtaa ctgatacagt aataatttga gagtttgttt gattggttac
     6721 ttttaaaaac gaattcaact cagctgttga atactgacca tcaaactctc tagatacttt
     6781 atctagaatt cttggacttt tgataatttc cgtatatgta ttaacggact gcaaactact
     6841 ttgaacattt tggaaagcta aatcacttga ggactttttc atgttaacta atatttgagt
     6901 agaagcagta tatttgtcag gcataacaaa aaaggttaat gccgcactta ctacaagaca
     6961 tattgccggt aaaataagca ataatttaat attcttcttt agaatattta atagttttac
     7021 taaatcaaac ttttctttca tggtttcctc cacataatca atcattgtat tcattatgta
     7081 tgttttataa atcggacaat tatatctagt ttaacgacca caaaaataca caactacatt
     7141 ttctctaatt ttttatataa atattttatc gtttaaaatt atatcatgat tcttgactgt
     7201 tatgtataat ttatttatat tttcgttcga tatataatat tgcccaactt caaatatcca
     7261 aacctatcag taataaacct agataccatc gtactctatc atagctttct tataatcgag
     7321 cagaagcatc atcattactt gattatttgc tctttacaac accgagcgta cccgtactcg
     7381 gtagttcaat accttgcgta atccgtcact gtgagtttgg ttaatgataa taaagcccac
     7441 atcttttaaa aagatgtggg taatttatat aatttttatt tacattttaa acttatataa
     7501 aaaagcgtct atgtcatgat ttaccatcac ataggcgctt atcaataaat tattaattat
     7561 tactttccat ttcatctaat ttatgcggat ttcctgtaat tagatgacaa cttattcttt
     7621 tcaggggaac attacatttt tataatatgt tcaaagacaa acttaaccat tcacaaatat
     7681 aaagaataat attatcaaat cattgaacaa atcgt
//
LOCUS       DAKSQE010000111         7628 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.111, whole genome shotgun sequence.
ACCESSION   DAKSQE010000111 DAKSQE010000000
VERSION     DAKSQE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7628
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.111"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            148..612
                     /locus_tag="O0V33_002207"
     CDS             148..612
                     /locus_tag="O0V33_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976824.1"
                     /translation="MKRLLGLLLVSTLVLSACGNDENQEESKKEVKSKEKKIEKEKEN
                     KSKKDKEKEVATQQQPDNQTVEQPQSQEQSVQQPQQQIPQNSVPQQNVQVQQNKKQKV
                     DLNNMPPTDFSTEGMSEQAQKQIEELSMQKDYHGLSQREYNDRVSEIINNDN"
     gene            671..1708
                     /locus_tag="O0V33_002208"
     CDS             671..1708
                     /locus_tag="O0V33_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCX8976825.1"
                     /translation="MKTRCYDGKKWQYEFKHEGKRYRKKGFRTKREANSAGLDKLNEL
                     RSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINR
                     QVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSE
                     QEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFI
                     DERKTNTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEGGSIINLHAINNALKSAC
                     RVNNIPIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEE
                     DKKTTKILESM"
     gene            1759..2591
                     /gene="sph"
                     /locus_tag="O0V33_002209"
                     /pseudo
     CDS             1759..2591
                     /gene="sph"
                     /locus_tag="O0V33_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            complement(2848..3867)
                     /locus_tag="O0V33_002210"
     CDS             complement(2848..3867)
                     /locus_tag="O0V33_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leukocidin/hemolysin toxin family protein"
                     /protein_id="HCX8976826.1"
                     /translation="MIKQVCKNITICSLALSTALTVFPASSYAEIKSKITTVSEKNLD
                     GDTKMYTRTATTSDTEKKISQSLQFNFLTEPNYDKETVFIKAKGTIGSGLKILNPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNSTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRG
                     GLTGNITKEKNYSETISYQQPSYRTLIDQPTTNKGVAWKVEAHSINNMGHDHTRQLTN
                     DSDDRVKSEIFSLTRNGNLWAKDNFTPKNKMPVTVSEGFNPEFLAVMSHDKNDKGKSR
                     FIVHYKRSMDDFKLDWNKHGFWGYWSGENHVDQKEEKLSALYEVDWKTHDVKLIKTIN
                     DKEQK"
     gene            complement(3889..4944)
                     /locus_tag="O0V33_002211"
     CDS             complement(3889..4944)
                     /locus_tag="O0V33_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leukocidin family pore-forming toxin"
                     /protein_id="HCX8976827.1"
                     /translation="MKNKKRVFIASSLSCVLLLLSAANTEANSANKDSQDQTKKEHVD
                     KAQQKEKRNVNDKDKNTPGPDDIGKNGKVTKRTVSEYDKETNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHRNETNASWLKYPSEYHVDFQVQRNPKTEILDQLPKNK
                     ISTAKVDSTFSYSLGGKFDSTKGIGRTSSNSYSKSISYNQQNYDTIASGKNNNRHVHW
                     SVVANDLKYGNEIKNRNDEFLFYRNTRLSTVENPELSFASKYRYPALVRSGFNPEFLT
                     YISNEKSNEKTRFEVTYTRNQDILKNKPGIHYGQPILEQNKDGQRFIVVYEVDWKNKT
                     VKVVEKYSDQNKPYKEG"
     gene            5376..6599
                     /locus_tag="O0V33_002212"
     CDS             5376..6599
                     /locus_tag="O0V33_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCX8976828.1"
                     /translation="MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLKDLFDKYDIKS
                     EILKVNEHRANFVAEIGSGSPILALSGHMDVVDAGNQDNWTYPPFQLTEKDDKLYGRG
                     TTDMKGGLMALVIALIELKEQNQLPQGTIRLLATAGEEKEQEGAKLLADKGYLDDVDG
                     LMIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNQFKEKYAE
                     LKKNDTKHELDVAPMFKSLIGKDISEEDANYASGLTAVCSIINGGKQFNSVPDEASLE
                     FNVRPVPEYDNDFIESFFQNIINNVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVAS
                     SYVDKDDIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKY
                     IDIFKEASIQYLKEK"
     gene            7038..>7628
                     /locus_tag="O0V33_002213"
     CDS             7038..>7628
                     /locus_tag="O0V33_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCX8976829.1"
                     /translation="MNKVHKPLYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFI
                     ASSAFTVTGLSPVDIGSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNR
                     FLIMVTWNIDEPGGVIKLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTS
                     VSAFNNAGFALFKNNLIDYSNDPIVIITISILIIFGG"
BASE COUNT         2649 a         1192 c         1221 g         2566 t
ORIGIN      
        1 tcacaaaaat tagatggcga taagttaacc aaagaagatt cattatgatt ttactaaaca
       61 aaaaaacgcc tactagtgtg aaaacgtatt gattaatagc gcctatatgg agttttaata
      121 taaaaagtaa gcaaaggaga aatgagaatg aaaagattgt taggtttatt attagtgagc
      181 acgttagtgt taagtgcatg tggaaatgat gagaatcagg aagaatctaa aaaagaagtt
      241 aaatcaaaag aaaagaaaat tgagaaggaa aaggaaaata aatcgaaaaa agataaggaa
      301 aaagaagttg caacacaaca acaaccagac aatcaaaccg ttgaacaacc ccaatcacaa
      361 gagcaatcgg ttcaacaacc gcaacaacag ataccacaaa atagtgttcc tcagcaaaat
      421 gtccaagttc aacaaaacaa aaagcaaaaa gttgatttaa ataatatgcc tcccactgat
      481 ttttctacag agggtatgtc tgagcaggct caaaaacaaa ttgaagagct ttcaatgcaa
      541 aaagactatc atggtctgtc acaaagagaa tacaatgata gagtttctga aattataaat
      601 aatgataatt gattccacag ggtagcccgc ctacccttat tattttttgc caattttgag
      661 gagggaacgc atgaaaacac gttgttacga tggtaaaaaa tggcaatatg aatttaagca
      721 tgaaggaaaa agataccgta agaaaggttt tagaacaaag cgtgaagcta attctgctgg
      781 actagacaag ttaaatgagt taagaagtgg ttttaatata gataactata taactcttga
      841 agaatacttc gaaaattgga ttaaaacata taaacaacct gttgttaaag aaaataccta
      901 ccgtcattat agaaatgcat tacaacatat acaaaaacat aaaataggta aaatggagtt
      961 atcaaagata aatagacaag tttatcagaa attcataaac gactattcaa aagaacacgc
     1021 aaaagaaact ataagaaaaa caaacggtgc tattcggtca gctttagatg acgcattata
     1081 tgatggactt atttttaaaa accccgctta taaagttaat tataaagccg gaaaacctac
     1141 gaagtcagaa caagaaaaat tcatctcggt aactgaatat gaaatactaa aagatcacgt
     1201 cagaaagaag agaactcgtt catcattagc gctattcata atgatttgta cgggttgtcg
     1261 tgtcagtggt gcaagaaata taaagattga gcatatcaac caagtgaaaa acactatatt
     1321 tattgacgag cgaaaaacca atacttcccc tagatatatc agtatcgcta aatctgatat
     1381 gaaacacatt atggacgtca taagtacatt tgcaattagc tatgatggtt acattttcaa
     1441 agaaggcgga tctataatta accttcatgc tatcaataat gctttgaaat cagcctgtag
     1501 agtcaataat ataccaatta ttacatcgca cgcattaaga cacactcatt gttcttattt
     1561 actagcaaaa ggtgtatcta tacattacat ttctaaaaga ttaggtcata aaaatatagc
     1621 aataactaca tctgtgtatt ctcatttgtt agaagaaaaa tttaatgaag aggacaaaaa
     1681 aacaactaaa attttagaaa gtatgtaatt tagggaccca ttagggactc caaacccaat
     1741 aaatactgtt gttacaaggt ttctatgtat ccaaactggg ggggcaatat aaacgcgctt
     1801 atttaatcgg acaatcttct tatattaaaa ataatgatgt cgtaatattc aatgaagcat
     1861 ttgataatgg tgcatcagac aaattattaa gtaatgtgaa aaaagaatat ccttaccaaa
     1921 cacctgtact cggtcgttct caatcaggtt gggacaaaac tgaaggtagc tactcatcaa
     1981 ctgttgctga agatggcggc gtagcgattg taagtaaata tcctattaaa gaaaaaatcc
     2041 aacatgtatt caaaagcggt tgtggattcg acaatgacag taataaaggc tttgtttata
     2101 caaagataga gaaaaatggt aagaacgttc acgttatcgg tacacataca caatctgaag
     2161 attcacgttg tggtgctgga catgatcgaa aaattagagc tgaacaaatg aaagaaatca
     2221 gtgactttgt taaaaagaaa aatatcccta aagatgaaac ggtatatata ggtggcgacc
     2281 ttaatgttaa taaaggaact gaagagttca aagatatgct taaaaacttg aatgtaaatg
     2341 atgttttata tgcaggtcat aatagcacat gggaccctca atcaaattca attgcgaaat
     2401 ataattatcc taatggtaaa ccagaacatt tagactatat atttacagat aaagatcata
     2461 aacaaccaaa acaattagtc aatgaagttg ttactgagaa accgaaacca tgggatgtat
     2521 atgccttccc ttactactat gtatacaatg atttctcaga tcattatcca atcaaagcct
     2581 atagtaaata gcactcaaca aaaaactcgc ttcgttctaa aaagtcgaag cgagttatat
     2641 tgttaaaatt tgaattgact aactttaaac tgatatctca tcgttttcat ttctttaact
     2701 tccactatac ctctacatgc ctcatatgca cctaacaata tcaagttagg ttatccataa
     2761 taaaagatac agtgtctcaa gctatctatc ccaaaacaaa taaatagtta cctactacgt
     2821 gatacgcagc aggcaacttg cgaaaactta tttctgttct ttatcattaa ttgttttaat
     2881 taattttaca tcatgtgtct tccaatcaac ttcatataat gctgataatt tttcttcttt
     2941 ttgatctaca tggttttcac cagaccaata gccccagaaa ccatgtttgt tccaatctaa
     3001 tttaaagtca tccattgatc gtttataatg aacgataaat cttgatttac ctttatcatt
     3061 tttatcgtgt gacattacag ctaaaaattc tggattaaat ccttctgaca cagttacagg
     3121 cattttattt ttaggcgtga aattatcttt cgcccatagg tttccgtttc gagttaatga
     3181 aaagatttca cttttcactc tatcatcact gtcattagtt agttgtctcg tatgatcatg
     3241 tcccatatta ttgatagaat gtgcttctac tttccaagct acacctttat ttgttgtagg
     3301 ttgatcaata agtgtgcggt aagatggttg ttgataacta attgtctctg aataattttt
     3361 ctcttttgta atatttccag ttaaaccacc acgattaatt gaaaaatctc cacctgtttt
     3421 atagccatac gtatatttaa cttctcttga ttcatcttga tttttaggcg caaaatcagt
     3481 cacatttgta ctgttgttgt tatcatcaac gttttgaatc gagactgaat aagaaccagg
     3541 ccatcttaat gtactattcc aataaccatt tggatttaaa attttcaatc cactgccaat
     3601 tgtacctttc gccttaataa atactgtctc tttatcataa tttggctcag taagaaaatt
     3661 aaattgtaag ctttgtgaga tttttttctc tgtatcactc gttgtagccg tacgtgtata
     3721 catcttcgta tcaccatcaa ggttcttctc ggaaactgta gtaattttag atttaatttc
     3781 tgcataagaa gatgctggaa atacagttaa agctgttgat agcgctaaac tacagattgt
     3841 aatattttta catacttgtt taatcatttg tcatccgtcc cttctacttt atccttcttt
     3901 atatggttta ttttgatcag agtatttttc aacgacttta actgttttat ttttccaatc
     3961 tacttcataa actacaataa atctttgacc atctttattt tgttctaaaa ttggttgtcc
     4021 ataatgtata ccaggtttat ttttcaaaat atcttgatta cgtgtatatg taacttcgaa
     4081 tcgtgttttt tcatttgact tttcattaga aatataagtt aagaattctg ggttaaatcc
     4141 gcttcttaca agcgcaggat atctatattt tgatgcaaaa cttaattcag gattttctac
     4201 agtagataat ctagtatttc tatagaataa gaattcatca tttctgttct taatttcatt
     4261 cccgtatttt aaatcgttgg caacaacaga ccagtgtacg tgtctattat tatttttacc
     4321 gctcgcaatt gtgtcgtaat tttgttggtt ataactgata gatttagaat agctattaga
     4381 tgatgttcgt ccaatacctt ttgttgaatc gaatttacca cctaagctat aagaaaatgt
     4441 actatctact ttcgcagtgg aaattttatt ttttggcagt tgatctaaaa tttcagtttt
     4501 agggtttctt tgtacttgaa aatcaacgtg atattcactc gggtatttta accatgatgc
     4561 gtttgtttca tttcgatgag attcaaactt taaatttgaa tgaattgacc cttgtttttt
     4621 gacaagtaag acattcttgt catatgttgg atcatcgata aagtcgaatt gtaaattttg
     4681 taaaatattt gtctctttat cgtattcaga tacagtacgc tttgtaactt taccgttttt
     4741 accaatatca tcaggtcctg gtgtattttt atctttatca ttcacattac gcttttcttt
     4801 ttgttgagcc ttatcaacat gttctttctt agtttggtct tgtgagtctt tattcgctga
     4861 attcgcttct gtatttgctg ctgataacaa taaaagtaca catgataatg atgacgcaat
     4921 gaatacgcgc tttttatttt tcattgttat aaccttcttt cttatattta atattgttga
     4981 taggtatcga catgtgaata atatcacaaa aactaagaat atatatttaa ctatttatta
     5041 aatgattttg tttatattat taaatacttt tttcgatata aaattaatgt gtgtacaaag
     5101 tgattaaatt agtaaatcgc attatttagt agttaatcac catggatcta tatgttatac
     5161 atgcttaaac taaatttttt tcatacaatc cgactggacg attcacaatg aaaaaaacac
     5221 ttactatcag tgattattag actactgata caaggtgcat attttaagat gtaatcatga
     5281 acatagcacg tcactttgtt acttaaaatt aatgtttaaa tacaataatt atgtttatat
     5341 aaatatatat actactttga aaaggtgtga gcttaatgac aacttttagt gaaaaagaaa
     5401 aaattcaatt actagcagat attgttgaac tacaaactga aaataataat gaaatagacg
     5461 tttgtaatta tttaaaagat ttattcgaca agtacgatat taaatctgaa attttgaaag
     5521 ttaatgaaca ccgcgccaat ttcgttgcag aaatcggtag tggctctccc atacttgcat
     5581 tgagcggtca tatggatgtt gttgatgcag gaaatcaaga taattggact tatccccctt
     5641 ttcaactcac agaaaaagat gacaaattat atggtcgagg cacaacagat atgaaaggcg
     5701 gtctgatggc tttggtcatc gcactcattg aattaaaaga acaaaaccaa ttacctcaag
     5761 gaaccattag attactcgct actgctggtg aagagaaaga acaagaaggc gccaaattat
     5821 tagcagataa aggctattta gatgatgttg atggtctaat gattgctgaa ccaactggat
     5881 ctggaattta ttatgcacat aaagggtcta tgtcatgtaa agtaactgca actggtaaag
     5941 ctgtccatag ctcagttcca tttattggtg acaatgcaat tgatacactg cttgaatttt
     6001 ataatcaatt taaagaaaaa tatgcagagc ttaaaaaaaa tgatactaaa catgagttag
     6061 atgttgctcc catgttcaaa tcattgatag gaaaagatat ttctgaagag gatgcaaatt
     6121 atgcatctgg tcttacagct gtatgttcga ttataaatgg cggtaaacaa tttaactctg
     6181 taccagatga agcttcactt gaatttaacg taagaccagt tcctgagtat gataacgact
     6241 ttatagaatc gtttttccaa aatatcatta ataatgtgga tagcaataag ctttcactcg
     6301 atattccaag caaccatcga cctgtaacaa gcgataaaaa tagcaaatta attactacga
     6361 ttaaagatgt agcttctagt tatgtagaca aagacgatat atttgtttcg gcgcttgtag
     6421 gtgcaacgga tgcttccagc ttcttaggag ataataagga caatgttgat ttagccattt
     6481 ttggaccagg taatccatta atggcacatc aaatcgatga atatattgaa aaagatatgt
     6541 atctgaaata tattgatatt ttcaaagagg cttccattca atatttaaag gagaaataag
     6601 aggtatactg tcagctgccc tattcgcgtg ctggcagttt tttattgcga attttaagtt
     6661 gatagaaata gcgatgaata ttgtttgaat gtaaatattc aaatatcaac aagcatattt
     6721 ccattgaata ggtgatgtaa aactgaaact atagcgttga tttgtcatac ttaaattgca
     6781 ttgtctgttg aaattgggaa tccaatttct ctttgttggg gccccacccc aacttacatt
     6841 gtttgtagaa tttcttttcg aaattctctg tgttggggcc ccgccaactt gcattgttcg
     6901 cagaatttct ttttgaaatt ctctgtgttg gggctcctga ctagaattga aaagtaatac
     6961 acatcagtaa catttctatt ttcatttatg atattatcta attattaatt tataatataa
     7021 aggagtatgc aattaaaatg aacaaagtcc acaaaccttt atatttttat ttaatgcttt
     7081 tcttctctac aactatcata ggtgcacttt tattgtattt acctttcact ggtaaaaagc
     7141 caatatcttt tttagatgcc ctatttatag cttcaagtgc atttacagtt actggcttgt
     7201 ccccagttga tataggatca cagtttaata tacttggtga gatagtaata ctattattaa
     7261 tacaaatagg tggtctaggt atcgtaaccg taaccctatt gacactagta tttttaaata
     7321 gaaagatatc aatgaaaaat agatttttga ttatggttac atggaatatt gacgaacctg
     7381 gtggtgttat taagctaatt aaacacttgg ctatttatag tttagtcact gaattaattg
     7441 gtatgatttg tttgtgttta tcttttatac caaaatttgg tataggcaaa ggattatttt
     7501 taagcttatt cacatcagta tcagctttta ataatgctgg atttgccctt tttaagaata
     7561 acttaataga ttattctaat gatccaattg tcattatcac aatctcaata ctcataatat
     7621 ttggaggt
//
LOCUS       DAKSQE010000112         7528 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.112, whole genome shotgun sequence.
ACCESSION   DAKSQE010000112 DAKSQE010000000
VERSION     DAKSQE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7528
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.112"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            217..1113
                     /gene="xerC"
                     /locus_tag="O0V33_002214"
     CDS             217..1113
                     /gene="xerC"
                     /locus_tag="O0V33_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCX8976830.1"
                     /translation="MNHIQEAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLQL
                     NTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKSLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLIVNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            1110..1655
                     /gene="hslV"
                     /locus_tag="O0V33_002215"
     CDS             1110..1655
                     /gene="hslV"
                     /locus_tag="O0V33_002215"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCX8976831.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            1721..3124
                     /gene="hslU"
                     /locus_tag="O0V33_002216"
     CDS             1721..3124
                     /gene="hslU"
                     /locus_tag="O0V33_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCX8976832.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLAVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            3149..3922
                     /gene="codY"
                     /locus_tag="O0V33_002217"
     CDS             3149..3922
                     /gene="codY"
                     /locus_tag="O0V33_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCX8976833.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            3973..4065
                     /locus_tag="O0V33_002218"
     CDS             3973..4065
                     /locus_tag="O0V33_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976834.1"
                     /translation="MAVVNEMTLMIMLKSFLYFCLIFNQIDIPH"
     gene            4264..5031
                     /gene="rpsB"
                     /locus_tag="O0V33_002219"
     CDS             4264..5031
                     /gene="rpsB"
                     /locus_tag="O0V33_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCX8976835.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            5065..5178
                     /locus_tag="O0V33_002220"
     CDS             5065..5178
                     /locus_tag="O0V33_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001791405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976836.1"
                     /translation="MGDKILMLITFFKKKIKANYKYSIRVLAILPIIMLKL"
     gene            5213..6094
                     /locus_tag="O0V33_002221"
     CDS             5213..6094
                     /locus_tag="O0V33_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCX8976837.1"
                     /translation="MATISAKLVKELREKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            6231..6953
                     /locus_tag="O0V33_002222"
     CDS             6231..6953
                     /locus_tag="O0V33_002222"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCX8976838.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            6972..7526
                     /gene="frr"
                     /locus_tag="O0V33_002223"
     CDS             6972..7526
                     /gene="frr"
                     /locus_tag="O0V33_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCX8976839.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
BASE COUNT         2851 a         1052 c         1492 g         2133 t
ORIGIN      
        1 tttaaataaa gtttatttaa aagacaatga aagaataaat agtaaggcta ttaataaaga
       61 cttttagtaa ttaatttaca aaagaggtat tctaattaac aggttttccg attagttaca
      121 attatttaat tctcaaaaga tttagaattg attatcaaat tactgtaagc cctttgctgt
      181 atatgctaca attcttattg atggagggta aatgtattga atcatattca agaagcattt
      241 ttaaatacat tgaaagttga acgaaatttt tcagaacaca cattgaaatc atatcaagat
      301 gacttaattc agtttaatca atttttagaa caggaacatt tacagttgaa tacttttgaa
      361 tacagagatg ctagaaatta tttgagctat ttatattcaa atcatttgaa aagaacatct
      421 gtttctcgta aaatctcaac gttaagaact ttctatgaat attggatgac gcttgatgag
      481 aacattatta atccatttgt tcaattagta catccgaaaa aagaaaaata tcttccgcaa
      541 ttcttttacg aagaagaaat ggaagcgtta tttaaaactg tagaagagga cacttcaaaa
      601 agtttacggg atcgagttat tcttgaattg ttatatgcta caggtatccg tgtttcggaa
      661 ttagttaata ttaaaaaaca agatatagat ttttacgcga atggtgttac cgtattagga
      721 aaagggagca aagagcgctt tgtaccgttt ggtgcttatt gtagacaaag catcgaaaat
      781 tatttagaac atttcaaacc aattcagtca tgcaatcatg attttcttat tgtaaatatg
      841 aagggtgaag caatcactga acgcggtgta cgatatgttt taaatgatat tgttaaacga
      901 acagcaggcg taagtgagat tcatccccac aagctcagac atacatttgc aacgcattta
      961 ttgaatcaag gtgcagacct aagaacagta caatcgttat taggtcatgt taatttgtca
     1021 acaactggta aatatacaca cgtatctaac caacaattaa gaaaagtgta tctaaatgca
     1081 catcctcgag cgaaaaagga gaatgaaaca tgagtaatac aacattacat gcaacaacaa
     1141 tttatgctgt aagacataat gggaaagcag ctatggctgg agatgggcaa gtaacgcttg
     1201 gtcaacaagt catcatgaaa caaacggcaa gaaaagtgcg tcgtttatat gaaggtaaag
     1261 tgttagctgg tttcgcaggt agtgtagcag atgcgtttac gttatttgaa aaattcgaaa
     1321 caaaattaca acagtttagt ggtaacttag aaagagctgc tgttgaattg gcacaagaat
     1381 ggcgaggcga taaacaatta cgtcaattag aagctatgct aattgtaatg gataaagatg
     1441 ctattttagt tgtcagtgga actggcgaag ttattgcccc agatgatgac cttatcgcta
     1501 ttggatcagg aggcaactat gcattaagcg caggacgtgc attgaaacgc catgcatcgc
     1561 atttgtctgc tgaagaaatg gcatatgaga gcttgaaagt agcggctgat atttgtgtct
     1621 ttaccaacga taatattgtt gtcgaaacac tataataatc agagcatgat aaataattat
     1681 gagcaattaa ttttagttaa aagacggagg aatgaaatta atggatacag ctggaataag
     1741 attaactcca aaagaaatcg tatctaaatt aaatgaatac atcgttggac aaaatgatgc
     1801 taaacgtaaa gtggcaattg ccctacgtaa tcgatacaga agaagtttat tggatgagga
     1861 atcaaagcaa gaaatttcac ctaaaaatat tttgatgatt ggaccaaccg gtgttggtaa
     1921 aactgaaatt gcaagaagaa tggccaaagt tgtcggcgca ccatttataa aagtagaagc
     1981 tactaaattt actgaagtag gttatgtagg acgagatgtt gaaagtatgg ttagagatct
     2041 tgttgatgtt tcagtaagat tagtcaaggc gcagaaaaaa tcattggtac aagatgaagc
     2101 aacagctaag gccaatgaaa aacttgttaa gttattagtt ccaagtatga aaaagaaagc
     2161 gtctcaaacg aataatcctt tagagtcact tttcggaggt gcaattccaa atttcggaca
     2221 aaataacgaa gatgaagaag aaccacctac tgaggaaatt aaaacaaaac gttctgaaat
     2281 taagagacaa ttagaagaag gaaaacttga aaaagaaaag gtaagaatta aagtcgaaca
     2341 agatcctggt gctttaggta tgctaggtac aaatcaaaat cagcaaatgc aagagatgat
     2401 gaatcaatta atgcctaaaa agaaagttga gcgagaagtt gctgttgaga cggcaaggaa
     2461 aatcttagct gatagttatg cggatgaact aattgatcaa gaaagcgcta accaagaagc
     2521 acttgaatta gcagaacaaa tgggtatcat ctttatagat gaaatcgaca aagttgcgac
     2581 gaataatcat aatagtggtc aagatgtctc aagacaaggt gtgcaaagag atattttacc
     2641 tatacttgaa ggtagcgtta ttcaaaccaa atatggtact gtgaatactg aacatatgct
     2701 gtttataggt gctggagctt tccatgtatc taagccgagt gacttgatac cagaattgca
     2761 aggtcgtttt ccgattagag ttgaacttga tagtttagcg gtagaagatt ttgtaagaat
     2821 tttgacagaa ccaaaattgt cattaattaa acaatatgaa gcattgcttc aaacagaaga
     2881 agttactgta aactttaccg atgaagcaat tactcgttta gctgagattg cttatcaagt
     2941 aaatcaagat acagacaaca ttggtgcacg tcgacttcat acaattttag aaaagatgtt
     3001 agaagattta tcattcgaag caccaagtat gccgaatgca gttgtagata ttaccccaca
     3061 atatgttgat gataaattaa aatcaatttc aacaaataaa gatttaagtg catttattct
     3121 ataaaaatat acaaaaggag aaaaattcat gagcttatta tctaaaacga gagagttaaa
     3181 cacgttactt caaaaacaca aaggtattgc ggttgatttt aaagatgtag cacaaacgat
     3241 tagtagcgta actgtaacaa atgtatttat tgtatcgcgt cgaggtaaaa ttttaggatc
     3301 gagtctaaat gaattattaa aaagtcaaag aattattcaa atgttggaag aaagacatat
     3361 tccaagtgaa tatacagaac gattaatgga agttaaacaa acagaatcaa atattgatat
     3421 cgacaatgta ttaacagttt tcccacctga aaacagagaa ttattcatag atagtcgtac
     3481 aactatcttc ccaattttag gcggaggaga aagattaggt acattagtac ttggtcgagt
     3541 acacgatgac tttaatgaaa atgatttggt actaggtgaa tatgctgcta cagttattgg
     3601 tatggaaatc ttacgtgaga agcatagtga agtagaaaaa gaagcgcgcg ataaagctgc
     3661 tattacaatg gcaattaatt cattatctta ttctgaaaaa gaagcaattg aacatatctt
     3721 tgaagaactt ggcggtacag aaggcctatt aatcgcatca aaagttgcag atagagttgg
     3781 tattactaga tctgtaattg taaatgcact acgtaaatta gaaagtgctg gtgtaattga
     3841 atcacgttct ttaggaatga aaggtacttt cattaaagtt aaaaaagaaa aattcttaga
     3901 tgaattagaa aaaagtaagt aaaacgatga gtctgggaca taattcaatg tcctagtcgt
     3961 aacgatgtta tcttggcagt agtcaacgaa atgactttga tgattatgtt gaagtcattt
     4021 ctttattttt gtctaatttt caatcaaatt gatattccgc attaacatga tagaattacc
     4081 tttaaattta tgaaaaaatg cttgaaaaac atttgcttta gacacggcat tatgttatat
     4141 ttattttcgg ctataaagcc aatacacaca tatcaaacgt gattgtgtaa agggtgcaat
     4201 atttgtatat tgtctttaca atagtttgct atggaggtaa ttaaccaata ggaggaattt
     4261 ataatggcag taatttcaat gaaacaatta ctagaagcgg gtgttcactt cggtcaccaa
     4321 acacgtcgtt ggaacccaaa aatgaaaaaa tatatcttca ctgagagaaa tggtatttat
     4381 atcatcgact tacaaaaaac agtgaaaaaa gtagacgagg catacaactt cttgaaacaa
     4441 gtttcagaag atggtggaca agtcttattc gtaggaacta aaaaacaagc acaagaatca
     4501 gttaaatctg aagcagaacg tgctggtcaa ttctacatta accaaagatg gttaggtgga
     4561 ttattaacta actataaaac gatctcaaaa cgaatcaaac gtatttctga aattgaaaaa
     4621 atggaagaag atggtttatt cgaagtatta cctaaaaaag aagtagtaga acttaaaaaa
     4681 gaatacgacc gtttaatcaa attcttaggc ggaattcgtg atatgaaatc aatgcctcaa
     4741 gcattattcg tagttgaccc acgtaaagag cgtaatgcaa ttgctgaagc tcgtaaatta
     4801 aatattccta tcgtaggtat cgttgacact aactgtgatc ctgacgaaat tgactacgtt
     4861 atcccagcaa acgacgatgc tatccgtgcg gttaaattat taactgctaa aatggcagat
     4921 gcaatcttag aaggtcaaca aggcgtttct aatgaagaag tagctgcaga acaaaacatc
     4981 gatttagatg aaaaagaaaa atcagaagaa acagaagcaa ctgaagaata atcaactgtt
     5041 gaatctgact tagatatagt ttaaatgggt gataagatat taatgcttat cacctttttt
     5101 aaaaagaaaa tcaaggcaaa ttacaaatat tcaattagag tattggcaat cttgcctata
     5161 ataatgctaa aattataata tataaaatga taacttattg gaggaataat gaatggcaac
     5221 tatttcagca aaacttgtta aagaattacg tgaaaaaact ggcgcgggta tgatggattg
     5281 taaaaaagcg ctaactgaaa ctgatggtga catcgataaa gcgattgatt acctacgtga
     5341 aaaaggtatt gctaaagcag ctaaaaaagc agaccgtatt gcggctgaag gtttagtaca
     5401 tgtagaaact aaaggtaacg acgcagttat cgttgaaatc aactctgaaa cagactttgt
     5461 tgctcgtaac gaaggtttcc aagagttagt taaagaaatc gctaatcaag tattagatac
     5521 aaaagctgaa actgttgaag ctttaatgga aacaacttta ccaaatggta aatcagttga
     5581 tgaaagaatt aaagaagcaa tttcaacaat cggtgaaaaa ttaagtgttc gtcgttttgc
     5641 tatcagaact aaaactgata acgatgcttt cggcgcttac ttacacatgg gtggacgcat
     5701 tggtgtatta acagttgttg aaggttcaac tgacgaagaa gcagcaagag acgttgctat
     5761 gcatatcgct gcaatcaacc ctaaatatgt ttcttctgaa caagttagcg aagaagaaat
     5821 caaccacgaa agagaagttt taaaacaaca agcattaaat gaaggtaaac cagaaaacat
     5881 cgttgaaaaa atggtggaag gacgtttacg taaatactta caagaaattt gtgctgtaga
     5941 tcaagacttc gttaaaaacc ctgatgtaac agttgaagct ttcttaaaaa caaaaggtgg
     6001 aaaacttgtt gacttcgtac gctatgaagt aggcgaaggt atggaaaaac gcgaagaaaa
     6061 ctttgcggat gaagttaaag gacaaatgaa ataatctgtc ataaagtaaa acaaggaaga
     6121 agacaccttt aatgttgctt tattaaaatg taaatcattc taataaaacg acaactgtgt
     6181 cttctttact tgtatatgtt acatatattc acgatagaga ggataagaaa atggctcaaa
     6241 tttctaaata taaacgtgta gttttgaaac taagtggtga agcgttagct ggagaaaaag
     6301 gatttggcat aaatccagta attattaaaa gtgttgctga gcaagtggct gaagttgcta
     6361 aaatggactg tgaaatcgca gtaatcgttg gtggcggaaa catttggaga ggtaaaacag
     6421 gtagtgactt aggtatggac cgtggaactg ctgattacat gggtatgctt gcaactgtaa
     6481 tgaatgcctt agcattacaa gatagtttag aacaattgga ttgtgataca cgagtattaa
     6541 catctattga aatgaagcaa gtggctgaac cttatattcg tcgtcgtgca attagacact
     6601 tagaaaagaa acgtgtagtt atttttgctg caggtattgg aaacccatac ttctctacag
     6661 atactacagc ggcattacgt gctgcagaag ttgaagcaga tgttatttta atgggcaaaa
     6721 ataatgtaga tggtgtatat tctgcagatc ctaaagtaaa caaagatgcg gttaaatatg
     6781 aacatttaac gcatattcaa atgcttcaag aaggtttaca agtaatggat tcaacagcat
     6841 cctcattctg tatggataat aacattccgt taactgtttt ctctattatg gaagaaggaa
     6901 atattaaacg tgctgttatg ggtgaaaaga taggtacgtt aattacaaaa taaatttaga
     6961 ggtgtaaaat aatgagtgac attattaatg aaactaaatc aagaatgcaa aaatcaatcg
     7021 aaagcttatc acgtgaatta gcgaacatca gtgcaggaag agctaattca aatttattaa
     7081 atggcgtaac agttgattac tatggtgcac caacacctgt acaacaatta gcaagcatca
     7141 atgttccaga agcacgttta cttgttattt ctccatacga caaaacttct gtagctgaca
     7201 tcgaaaaagc gataatagca gctaacttag gtgtcaaccc aacaagtgat ggtgaagtga
     7261 tacgtattgc tgtacctgcc ttaacagaag aacgtagaaa agagcgcgtt aaagatgtta
     7321 agaaaattgg tgaagaagct aaagtatctg ttcgaaatat tcgtcgtgat atgaatgatc
     7381 agttgaaaaa agatgaaaaa aatggcgaca ttactgaaga tgagttgaga agtggtactg
     7441 aagatgttca gaaagcaaca gacaattcaa taaaagaaat tgatcaaatg attgctgata
     7501 aagaaaaaga tattatgtca gtataaaa
//
LOCUS       DAKSQE010000113         7282 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.113, whole genome shotgun sequence.
ACCESSION   DAKSQE010000113 DAKSQE010000000
VERSION     DAKSQE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7282
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.113"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(121..1494)
                     /locus_tag="O0V33_002224"
     CDS             complement(121..1494)
                     /locus_tag="O0V33_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HCX8976840.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRMSNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVQKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
     gene            complement(2167..2397)
                     /locus_tag="O0V33_002225"
     CDS             complement(2167..2397)
                     /locus_tag="O0V33_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976841.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
     gene            complement(2428..3364)
                     /locus_tag="O0V33_002226"
                     /pseudo
     CDS             complement(2428..3364)
                     /locus_tag="O0V33_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
     gene            complement(3800..4465)
                     /locus_tag="O0V33_002227"
     CDS             complement(3800..4465)
                     /locus_tag="O0V33_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCX8976842.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFEGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGDQDIKDALTQFEN
                     ETD"
     gene            4617..4937
                     /gene="czrA"
                     /locus_tag="O0V33_002228"
     CDS             4617..4937
                     /gene="czrA"
                     /locus_tag="O0V33_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HCX8976843.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            4939..5919
                     /gene="czrB"
                     /locus_tag="O0V33_002229"
     CDS             4939..5919
                     /gene="czrB"
                     /locus_tag="O0V33_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HCX8976844.1"
                     /translation="MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLA
                     NSLALLSDGIHMFSDTFSLGVALIAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFV
                     ISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAF
                     LHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEG
                     TPSDVDIDEVIATIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLE
                     NIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHSHHAHHHAHVH"
     gene            6185..7276
                     /locus_tag="O0V33_002230"
     CDS             6185..7276
                     /locus_tag="O0V33_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCX8976845.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     VQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLVGIAITTQSIIIIVVLLIKAIITWLLLIPIFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFKFFIGILLLNLVIILFKTPVGYLISFNTQSLSQSVAENIIRVYTVITI
                     ILFVVIHAVILMGIVQYYLKRGQKITKDKVKTADKNKKVVTEPKNTKVENDKVTTSVE
                     TKTEKAQDSLNDNTTKTMTSDKPEDNQPK"
BASE COUNT         2391 a         1304 c          988 g         2599 t
ORIGIN      
        1 ctatcataag ctcttaatta gctcttatgg ttgattaccc gtcatttttt ctttatacgg
       61 ttattcaaat atgcaatttt taatctttcc ttacttcttt tcaaaaacta ccatatatta
      121 ttagtcatgt ttgactttga ctatacgtgt gtattttaaa aactgctctg aatatttctc
      181 cttaatttcc tctaaactat catatgtaat acctacaata tgccaattag gattgaaata
      241 atagaaagaa tctttttgtc gtgaccattt aatcatatta ccgtctttat tataacgtgg
      301 taatgttacc tcatagcctt ctaacacatt aatatacgtt tggaaaatgt ctgtatctat
      361 acgattaaaa ttatcaataa ctaaataact tttatccttt ttaggcatta attgagtaat
      421 aaatccttca cgatagtata atgcccctgt ttcatttggt aaatacttgc cataaagcat
      481 atcttctgaa aaatcaggat gacctgttgt tataacagga tttgcattcg ctttttgcaa
      541 taagtcatta gccgctttta agccttcctc ttttcgatct acaacaagta aaataaatgg
      601 ctttaacgct tcttcttttt cactttcaag catatctggt ttttgaacca tttcaaatgg
      661 agatttcaat ccattattat cgctcatttc aataattgca tcatactgtg cttgtgacat
      721 acttgcaata gcctgttttg cattttcttg aaggaaatat aaatttttca atttaggatg
      781 cttatttaat gtacttaatg taatcggcgt aatgtctttc tcataagaca cttcaatcac
      841 tgtactattt gttctaccag gaattggtgg tttttcatga atatgctttg atacttctcc
      901 aattccaacg acagattgat ttttcgttcg attataaaaa ataatattgt cgccttcttc
      961 taactgagta taaaaatgat aaccattacg tttaataccg ttgtacgtgt gcgtataaat
     1021 cgtatattgg tttccaggtt caaattcttc agtttcagct aaaaagaaat aacgtggtat
     1081 cttaatttcg cctttaccaa gaccacttat taaatcaaac tcttctgcag tgatttggtt
     1141 gaacaatgtc tctttcatat tactcatacg aaattccaaa gcttcactac gctttaaata
     1201 atctgctgtt aatggtttca attgttcatt aaaacgaaac tgtacacgta ttttattttg
     1261 tgcacctgtt tcaacactaa taatttcacc acatccaagt agtccagtat ccgtctgaac
     1321 ttgataaaag atgacttgat ctcctacttt agccttttta aacgctctaa atccttgaga
     1381 tggattaaaa tgtgcgcctg attcaaataa agctgtttgt cctactaacg gttcattatg
     1441 attccaacgg ttatatccac aattcaacca aaaataattc gtttctgctg tcatcttaat
     1501 actccttaac ctgaataaat tttagaaaca ctatgaatta cattctttta atgtttctta
     1561 tgcagttggg cgcgtatacg atcaactgta tcccctttgt tcactgcgat tttaatcgca
     1621 tttcctataa cattgtagcg cctaggacat taatttacgt cccagaccct tatcgttttc
     1681 acttctaagt aagtcgaact attttgcttt acaacaagtg cgactctata tgcagttgga
     1741 cacacacata cgagcaactg tatacctttt aatcagtttt ctatatttta tttattatat
     1801 atgtcttaat gataaaaatt gttacaaaca gtttaacata tttagctacc tattttgcat
     1861 ataaaaaaca tgcttgcata cactatgcaa taaaaattac aaaattatat atgataccac
     1921 tatgcttgct tatctctata gcgccattga tacgcatttt taaatatcta tacgccttta
     1981 gaattttagc atgtcttaat tttcattaaa tattaattac ttcattttat atacaccaac
     2041 aaaaaccccc tcactactat gcaagtgaga ggttatgttg atgtgcttta ttttcaacaa
     2101 tgtcattaac acaatcatat ttctgctgtt aatgcactgt actatattca aacgcgctat
     2161 cgactattaa tttgaagcta caaaggtacc gcataatggc agcacattaa ttaacaatgc
     2221 gctaaacttt aatgaggcgc tgatttgctt gtctttaaaa gtcaacatag cactgataat
     2281 tcctaaaata gccactatac gtggaattgt catgctcgga tactgcggta tatgtaagaa
     2341 agcaccaaga cctgctatca caccacatac taacgcaata tatagaaatt gcttcaaatc
     2401 attcactcct aaattgttat tacactatta cacatagcta atcatcaatg tgaaatcacc
     2461 ttcaaagaca ctatccaaat cttcagaagt caaaataaag tttgtaccag tagtcagttt
     2521 gaaaatttca ccatcgacaa tcatttgccc ttcaccttcc aacactgtaa ctaaacagaa
     2581 ctctctaggc ttcatataat ttaacgtgcc agaaatttcc catttaacca atgtaaagaa
     2641 atcattcgat acaatgtgtg tacacttatg gttctcaata atttcacttt caggcaaaat
     2701 attaggtaat ggtgcattgt actgaataac gtctaaagct ttttcaatat ttaacggtct
     2761 accattatat tgattatctt gacgattgaa atcataaagt ctatatgtaa tgtctgacga
     2821 ttgcatcgtt tcgtatgcta gtattcctga agaaatagta tgtactgttc ctgctggaat
     2881 aaaatagaat tctccaggtt ttactttaat atatctaagt atcgactcta tcgttccgtg
     2941 ttgaacatga ttcgcaactt cttctctaga ctctgctaat gtccctataa ctatttctgc
     3001 atcttcttca gcatctataa tataccaaca ttcagatttg ccatattgcc cattttcatg
     3061 ttcaaaagca taagaattat cagggtgcac atgaatagaa agtgattctc ttgcatccac
     3121 tattttagtt agaagcggaa aatctttgct tgggaaatca ccaaacaatt cacgatgttc
     3181 tgaccaaata cggtctaatg tttgaccttg atatggtcca ttaataatct cgctcgtacc
     3241 atttggatgt gctgacacac caacattccc cagttgtatc attgtctaat tgatatccaa
     3301 actcacttag acgttgaccg ccccataatt ttgtttttaa aattggttgt aaaaataatg
     3361 gcattgttgc acctccattg tgattaagta agcaatagaa ctctgatgtt gttgttccat
     3421 tatattttga ttttgttctc atttatatcg tattataaac ttccacattt caaattaact
     3481 attagtgatt gtaccatatt tactaacatt gcagtactgc caattaaagc gcttcactta
     3541 aatttacagt actttaacat ttttaaaatt ttatagcata gagattatat ctctcttaca
     3601 tttgtacata tttctcttta aatttactcg cccattatac caattaataa acaactttaa
     3661 tagttgtgcc atacattgtt caaattcatt gtaaaacaca tagacaatac gtacttattc
     3721 atacttataa ttcatcatta tcaaaaaaat aacgagttac gaaaaagtaa cccgcttcaa
     3781 atcatattta ctatccttat taatccgttt cattttcaaa ttgagttaaa gcatctttaa
     3841 tgtcctggtc accactaata atttgaaact cttggtgatt aaaatgattg gatgtgacaa
     3901 tttcttttaa tactgtcgca acatcttctc taggaatttc acctttacct tcaaaatatt
     3961 gtgctgcttc tatctttcca gatcctgctg catttgtaag tgcccctgga tgtaaaattg
     4021 tataattcaa acctgaacgt cttaaatagt catcagcgta atgtttagca attgtatatg
     4081 gctttaaatc accgctatca tcaaaagcct gacgtctcga atcatatgtt gaaaccatga
     4141 catagtgttt aatattagcc tctttactcg caatcattga tttaacagca ccatctaaat
     4201 cgacaataat tgttttatct gcacccgtat tccctccaga acctactgaa aagataactt
     4261 tatcgaatgg tttaaacgtc tcagttaaag tctctattga atcattttca acatcaacaa
     4321 gaattgcttt cataccttgt gattttaacg cattaagttg atcggattgc ctaacacctg
     4381 cagtaaatgg tacattttct tttgctaatt gttgcactag taacgaacct acaccgccat
     4441 tagcacctat aaccaaaata ttcatttaca acactctcct atttattatt ccctatgcca
     4501 taccacttta tgagatatgt aaaacttgtt acaactataa aaatcaattg acatactact
     4561 gggaacgtat taaattaata tatgaacaaa tattcatatg aaaggattgt catataatgt
     4621 cagaacaata ttcagaaata aatacagata cattagaacg cgtaactgaa atcttcaagg
     4681 cattaggcga ttacaatcga atacgtatca tggaattgtt atcagtcagt gaagcaagtg
     4741 ttggtcacat ttcacatcaa ttgaatttat ctcaatcaaa tgtctcgcac caattaaaat
     4801 tacttaaaag tgtgcatctt gtgaaagcaa aacgacaagg ccaatcaatg atttattcat
     4861 tagatgacat ccacgtagca actatgttaa agcaagccat acatcacgcg aatcatccta
     4921 aagaaagtgg gttataatat gtctcattca catcatcatc atgaccatat gcatagtcat
     4981 gtaactacaa ataataagaa agtattgttt atatcgtttt taataatcgg tctatatatg
     5041 tttatcgaaa tcatcggcgg tctccttgct aacagcttgg cattactatc tgacggtatc
     5101 catatgttta gcgacacatt ctcattaggt gttgctttaa tcgcatttat atatgctgaa
     5161 aagaatgcca caactacaaa aacatttggt tataaacgtt tcgaagtact cgcagcgtta
     5221 tttaacggtg taacgctttt tgtaataagt attttaattg tttttgaagc gattaaacgt
     5281 ttctttgttc cttctgaagt ccaatctaaa gaaatgttaa tcattagtat tattggttta
     5341 attgtcaata tcgttgttgc attctttatg tttaaaggcg gcgacacttc acacaattta
     5401 aatatgcgtg gtgcttttct acatgttatc ggagacttat taggttcagt tggcgccatt
     5461 actgcagcta ttttaatttg ggcatttgga tggacaatcg ccgatcctat cgcaagtatt
     5521 ttagtttccg ttattatttt aaaaagcgct tggggtatca caaaatcttc aattaacatt
     5581 ttaatggaag gcacaccaag tgatgttgat atagatgaag ttatagctac tattaaaaag
     5641 gattcacgaa tacaaagtgt gcatgattgc catgtttgga caatttcaaa tgatatgaat
     5701 gcattaagtt gtcatgttgt tgtagaccat acattgacaa tgaaagaatg tgaattatta
     5761 ttagaaaaca ttgagcatga tttattacat ttaaatattc accatatgac tattcaatta
     5821 gaaacgccta atcacaaaca tgatgaatcg attatatgtt caggaacaca tagtcattca
     5881 catagccacc atgctcatca tcacgcgcat gtacattaat aattttaacc tactgccatt
     5941 gcatcgatta aacttttcaa tggcagtagg ttttttatgt ctttatggcg acttatttgg
     6001 tctttgatga tgcaatgttt attaacaaat tttcaactat tatttcttac attagtcata
     6061 tttttgacaa tttactatta taattctcta actttagtca ctttaattaa tttttattag
     6121 atattaatat gaaaataacg tgttttttgt tattgtattt atatgaagaa aaggaggcac
     6181 cactttgttt acttacttta aatcagcatt taaaaatgct aaaccacaat tattaataac
     6241 attgatatat gcacttatag catttgctgt aattgcagtt gtatatttac ttgctaattt
     6301 ccagcttgct aagtatgtac aaactattgc aatttattca caatttgggc aaaaaccacc
     6361 agtagatgca tatttaaaag ttatcgctgt attacttata gcagctgtag tttcactgtt
     6421 tgttttagtt caaattttta tcggaattac aaatgttatg aaacgagcaa tgagtcatga
     6481 aaaagtaaaa tttaccgatt tatttattgc atttaaaaaa ggtaattatt taaagagcgt
     6541 tctgatcggc cttgtatcta tagcaatgat tatcgttctt tcacttctga catcattact
     6601 atacaaatta ttttctccag tatcagaaat gattatgaat tcagtacaat catcatatgc
     6661 agatagtaca cacctggttg gtattgctat tacaactcaa agcatcataa tcattgttgt
     6721 tttattgatt aaagcaatta ttacttggtt attgttaata cctattttca actttatgac
     6781 tagtttcgtt gaatcaacta acgataaagt aaaaacacac ttagctaatg gatttaaagc
     6841 aatgaaaaat ggtcaaaaaa catttttcaa atttttcatt ggaatactat tattaaactt
     6901 agttattatt ttatttaaaa cacctgtggg ttacttgatt tcatttaata cgcaatcact
     6961 ctctcaaagt gttgcagaaa atatcataag agtttataca gtaataacaa tcattttatt
     7021 cgttgttatt catgcagtca ttttaatggg tatcgttcaa tattacttaa aacgtggtca
     7081 aaaaattact aaagataaag taaaaacagc tgacaagaat aaaaaagttg tcactgaacc
     7141 aaaaaataca aaagtagaaa atgataaagt gacaacatct gttgaaacga aaacagaaaa
     7201 agcacaagat tcattaaatg ataatactac aaaaacaatg acttcagata aaccagaaga
     7261 caaccaacca aaataaattt ta
//
LOCUS       DAKSQE010000114         7238 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.114, whole genome shotgun sequence.
ACCESSION   DAKSQE010000114 DAKSQE010000000
VERSION     DAKSQE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7238
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.114"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(86..1447)
                     /locus_tag="O0V33_002231"
     CDS             complement(86..1447)
                     /locus_tag="O0V33_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCX8976846.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAYKTQFGHASFTNLYEHGVFPKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKTPDLHKKA
                     TYKLFGGKYMGYLIFVFFIFVFGLLFINVETRRAIYFIPIWFILLGFMYLRYKRIAAK
                     SNN"
     gene            1795..2916
                     /locus_tag="O0V33_002232"
     CDS             1795..2916
                     /locus_tag="O0V33_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HCX8976847.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPIKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            2965..4233
                     /gene="icd"
                     /locus_tag="O0V33_002233"
     CDS             2965..4233
                     /gene="icd"
                     /locus_tag="O0V33_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCX8976848.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALTEFGDQVFTWQQYDEIVEKEGRDAANVAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLERLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            4623..5324
                     /locus_tag="O0V33_002234"
     CDS             4623..5324
                     /locus_tag="O0V33_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCX8976849.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVEN
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKE"
     gene            5327..6988
                     /locus_tag="O0V33_002235"
     CDS             5327..6988
                     /locus_tag="O0V33_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HCX8976850.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKEIVKQQNIDLLITKNDKKVFSSGKLKPTKTIDNYSQKKDV
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTEIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
BASE COUNT         2623 a         1030 c         1348 g         2237 t
ORIGIN      
        1 aggcaagtac gcaatttaat attataagtt gcatataaaa acccctgttg aactttaata
       61 tattcaacag gggtaaaact tgttgttagt tatttgattt agcagcgata cgtttatatc
      121 ttaagtacat aaatcctaat agtataaacc aaatcggaat aaagtatatc gcacgtcttg
      181 tttcaacatt gataaacaat aggccaaaca caaatataaa gaatacaaaa atgagatagc
      241 ccatatattt tccgccaaat aatttatacg tagccttttt atgaagatct ggagttttac
      301 gactataatt gatataagcg atgataatca gtccccaaac aactaagaat aatactgtag
      361 agattgttgt cacatacgta aataccttag tagcatcagg gaaaatatag tttaataatg
      421 ctgccacgag taacaatgct gatgaagcaa agatcgctac atgtggaacg ccatacttat
      481 tcgtcttaga gaaattcggt ggtgcttgtt gttgacttga taaaccgaac agcatacgac
      541 tattcgagaa aataccactg ttacatgatg aagcagcagc agttagtact acaaaattaa
      601 tcaagcctgc agcaaatgga attccaatta atgcgaataa ttttacgaat ggactgttat
      661 caggatcaac ttgttgccaa ggaataatcg acatgataac cgctagagca ccgacataaa
      721 atattaaaat tctaatagga acactattaa tcgcttttgg tattgtttta acaggatctt
      781 tcgtttcacc tgccgtaaca ccaatcattt cgattcccac gaatgagaat aaggccattt
      841 ggaatgacat aaagaatcca gatgctcctt tagggaatac accgtgctcg tataggttcg
      901 tgaaactcgc atgtccaaat tgagttttat atgcaaatag aatcataacg aaacctacga
      961 caattaaacc aatgattgtt gctattttaa tgatagagaa ccaaaattct aactctccaa
     1021 atagtctcgc actcaataag ttaaaagaca ttaataacaa tacgcaaaaa agtgcactaa
     1081 tccagtttgg aatttctggg aaccagaagc ttacatactt tgctacagct gttacttcag
     1141 ccatacctgt aataatccaa cagaaccagt atgtccaacc ggttacgaat cctgcaaaag
     1201 gcccaatgta tgtattggtt acatctgcga aagatttaaa ttcagtattt tgaatgatga
     1261 tttcacctaa acctcgcata aacataaata acataaatcc aataatgata tatgttagta
     1321 atatagaagg gccagttaat gcaatcgttt gaccagcgcc taagaatagc cctgtaccaa
     1381 ttgcgccacc aatagcaatt aattgaatgt ggcgattgct cagttccctt tgtaattttt
     1441 cagccataat acatctccct taaatataga tatgtttatt atgcacttat attaggatat
     1501 atacaattat tttcggtaaa aatgtgtaaa attccatgtt aatatacttt ggattttata
     1561 atcatatata ataaccaatt gaaaatttta ttctatgcta aaagttggtg atgaatataa
     1621 tcttgaaaaa gctttaatgg tgctatttgt ggccattctg tgacatttac atagatttac
     1681 aaaataattg ttgcacatat aatgccagtt tttatatttc acaaacgaaa tgcgtttagc
     1741 tataatatta gttgaaagcc atttcataaa gaaacagtaa aggggaaatt tatcatggca
     1801 gaattacaaa gaggtttaga aggggttatc gcagcggaga ctaaaataag ttcaattatt
     1861 gaaagtcaat tgacttatgc cggctatgat attgatgatc tagctgaaaa tgcgcaattt
     1921 gaagaagtta ttttcctatt atggaactat agattgccaa atgaagaaga gcttgctcat
     1981 ttaaagggga aattaaatca atacatgaca ttaaatcctc gtgtgtatac acattttgag
     2041 gagtatgtta cagatcacgt gcatccaatg acagcattac gtacgtcatt atcatatatt
     2101 gcacatttcg atcctgatgc tgaaaatgaa tcagatgaaa atcgttatga aagagcaatg
     2161 cgtatacagg ctaaagtagc atcattagtt acagcgtttg ctcgagtaag acaagataaa
     2221 gaaccaatta agcctaatcc tgacttaagt tatgcagcaa acttcctata tatgttacgt
     2281 ggggaattac caacagatat agaagtggaa gccttcaata aagcgcttat tttacacgct
     2341 gatcatgagt tgaacgcatc tgcatttaca gcacgttgtg cggtatcttc attgtcagat
     2401 atgtactcag gtattgtagc agctgtaggt tctctgaaag ggccattaca tggtggtgca
     2461 aatgaacaag ttatgacgat gttatctgag attgggtcaa ttgaaaatgt tgatgcttac
     2521 ttagatgaaa aatttgctaa taaagataaa gtaatgggct tcggtcatcg tgtatataaa
     2581 gatggtgatc ctagagcgaa atatttaaga gaaatgagcc gtcaaattac gaaagacgct
     2641 ggtcgtgaag aattatttga aatgtcagtg aaaatggaaa aacgcatggc agaagaaaaa
     2701 ggattaattc caaatgttga tttttatagt gcgagtgttt atcactgtat ggaaatacct
     2761 catgacttat tcacgccaat ctttgctgta agtcgttctg caggatggat tgctcatatt
     2821 ttagaacaat ataaagataa tagaattatg cgtcctagag caaaatatat tggcgaaacg
     2881 aatcgtaagt atattccgct tgaagaaaga aaataatcaa tacaaattaa aaatgaagat
     2941 gtaaaatttg gaggtaaaat aactatgact gcagaaaaaa ttactcaagg aactgaagga
     3001 ttaaacgtac ctaatgagcc aattatccca tttattatcg gtgatggaat tggaccggat
     3061 atttggaagg cagcaagccg agttatagat gctgctgttg agaaagccta taatggcgaa
     3121 aaacgcattg aatggaaaga agtgctagct ggtcaaaaag catttgatac aactggcgaa
     3181 tggttacctc aagaaacact tgatacaatt aaagaatatt taattgctgt taaaggacct
     3241 ttaacaacac caattggtgg tggtattaga tcattaaatg tagctttacg ccaagaatta
     3301 gatttattta cttgtttaag accggtacgt tggtttaaag gagtaccatc acctgttaaa
     3361 cgtccacaag atgttgatat ggttattttc cgtgaaaata ctgaagacat ttatgctggt
     3421 attgaattta aagaaggaac aacagaagtt aaaaaggtaa ttgatttctt acaaaacgaa
     3481 atgggtgcga caaatattcg attcccagaa acttcaggta ttggtattaa gccagtttct
     3541 aaagaaggta ctgagcgatt agttagagca gcaattcaat atgctatcga taataaccgt
     3601 aaatcagtta ctttagttca taaaggtaat attatgaaat ttacagaagg ctcatttaag
     3661 cagtggggtt acgatttagc attaactgaa tttggtgatc aagtatttac ttggcaacaa
     3721 tatgacgaaa ttgttgaaaa agaaggcaga gatgctgcta atgttgcaca agaaaaagct
     3781 gaaaaagaag gcaagattat cattaaagat tctattgctg acattttctt acaacaaatt
     3841 ttaactcgtc cagctgagca tgatgttgta gcaactatga acttgaatgg tgactacatt
     3901 tcagatgcat tagctgcaca agttggtggt attggtattg cgccaggtgc aaacattaat
     3961 tatgaaacag gtcatgctat ttttgaagca acacatggta cagctccaaa atatgcaggt
     4021 ttaaataaag tgaatccatc ttcagtaatt ttaagttctg tattaatgtt agaacgttta
     4081 ggatggcaag aagcggcaga taagattaca gattcaattg aagatacaat tgcttcaaaa
     4141 gttgttactt atgactttgc ccgtttaatg gatggtgctg aagaagtttc tacatcagca
     4201 tttgcagatg aattgattaa aaatttaaaa taagcaaaat aaacttaggt gaacctagca
     4261 actttaattg ctaggttcac ctttttaaat gaattgaagt tggcagcagg atagaaatga
     4321 taaagagtca cgaatgattg attatgtagt gtttcttaca cattaactaa agcgaatgtg
     4381 tatataagct taggaataaa tgagtaaaac tcatgcatag gcagtgggat agaaatgata
     4441 ttttcacaaa atttatttcg ttgtcccaga gtatgtcatg caaattatag aaggaaataa
     4501 aagaagtatt ttatttatgt gtaaagtaat gtaaaaaatg tgtaaagata atgtgtagga
     4561 tgttattttt ataaatagtt cggttataat gtaaatataa ttataagtta gggaggcata
     4621 ccatgtcgca aaaagtgttg gtagtagatg acgaacattc aatcgtaaca ttacttaaat
     4681 ataacttaga aacagctggt tatgaagttg ttgtcgcatt tgatggtgat gaggctttag
     4741 agaaggtaga aaatgaacag ccagatttaa ttattttaga tgttatgcta cctaaaaaag
     4801 atggcattga cgtatgtaag actgtaagaa caaataaaaa tttagtccct atattaatgt
     4861 taacggcgaa aaatgatgaa tttgatcggg tattaggttt agaattaggt gccgatgact
     4921 atatgactaa gcctttttca cctagagaag ttgttgcccg tgtgaaagcc attttaagac
     4981 gttctcaatt tgtaaaagaa gtagaagaga ttgacgatga tattaccatc ggatcgatac
     5041 gtattagacc cgattatttt gaagtatata agcataatga gctattggag ctaacaccga
     5101 aagaatttga actgttgctt tatttgatag aaagacaagg cagagtcatt acgagagagc
     5161 atatgttaaa tacggtatgg aattatgaat ttgcaggaga ctcaagaatt gttgatgtac
     5221 atatcagtca tttaagagat aaattagaag ataatcctaa gaagccacaa cttattaaaa
     5281 ctgtgcgtgg cttaggctat aaattggaaa gacctaaaga ataatgatga agtttcacca
     5341 ccgcttaatg ttattgataa gcagtatatt aattatcagt tttgttacat taggcattat
     5401 tattcacgct tccatcacat atgttttatc agaaaaacac gaacaagctc tagcaacaga
     5461 tgcaagatca tatatttatt tagtccaaga taatgagatt gataaagtta aagaaattgt
     5521 aaaacagcaa aatattgatt tattgataac taaaaatgat aaaaaggtgt ttagtagtgg
     5581 caaactgaaa cctactaaaa caattgataa ttacagtcaa aaaaaggacg tttttatttt
     5641 taataagaag gttaacggtt atcatgtatg gattaaaggt tacaataacg atattacaga
     5701 aatgcattgg acgttgtgga aatatcttat attgacatgt ttagttgtac ttatttgtct
     5761 gtattttgca agccgtagtt ttaagcgtac tttaatccgt cccattcaag aagttactta
     5821 tgcaacacaa ttgttggcaa atggttatta tcacatacgt gtacctgaaa gtaatgttgt
     5881 agaaactaaa gcgctttttg tgtctaccaa tgatttagca cgacgccttc aaaagcttaa
     5941 taatgagcaa aaaattcaat ccaaccgttt aaaaacaacg attgaaaata ttccaagtgc
     6001 tattttaatg atagatcgaa atggaaaaat cgttgttgca aataaagcgt attatgaaca
     6061 gttcaacatc tcgcataata ttgaacaagt aggctatcac ggctatgtta atactgaaat
     6121 tgagcaatta attttagaaa gttttaaagt tgaaaaacct atctatgaac aattggaagt
     6181 tgcaataaac caagtccatg ccaagtactt tgatatttct tgtgttccga ttttaactag
     6241 atctcaaaaa agtttgcaag ggattttagt tgtgatgcat gacataacaa atctgaaaca
     6301 acttgaaaat ctaagacgtg aatttgtagc aaatgtttca catgaattaa aaacacctat
     6361 tacttcaatt aaggggtttg ctgaaacact tattgatggt gcaaaaaatg atgcggaatc
     6421 attagacatg tttttgaaca taattttaaa agaatcaaat cgaatagaat ctttagtaac
     6481 agatctgtta gatttatcgc atattgagca acataccgag ttagatacag actatatgaa
     6541 tttatcagat ttaacgcggc gtattattga taatatgatg actcaagcca atcaaaaaaa
     6601 tatttccatt catactgaaa ttgaaaaaga tgtcattgtt aaagcacaag aaagtaaaat
     6661 tgctcaagtt atcacgaatt tgttaacgaa tgcaattaac tattcttatg aagatggaga
     6721 tattaacgtt cgtgtgtatc gagatgactt tcgtgtcatt ttcgaagtac aagattttgg
     6781 tataggtatt aaattagaag accaacaacg tatttttgaa agattttata gagtagataa
     6841 agcacgaagt agagattctg gtgggacagg tcttggatta tcaattacaa agcatatagt
     6901 agaggcccac caaggcaata ttgaagtgaa tagtcaagtt ggcaaaggct caacgttcaa
     6961 agttattcta aaagattata aagaataaaa attaaaagtg gtaacagcgc gtgtattttt
     7021 cacgaagttg ttgctgcttt tttatttcgt tcaaatctct attggtatta aattagagtt
     7081 aatgaagtgg aatatattcg gttgtcaatt ttgtcatttg catatgtcgt tttgtaagtt
     7141 ataagtaata gattgttgtg aatcaagcaa ttttataata tatttattat aataaagaaa
     7201 ttttagtaac acgcttaaaa tagatgctta attaaaaa
//
LOCUS       DAKSQE010000115         7124 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.115, whole genome shotgun sequence.
ACCESSION   DAKSQE010000115 DAKSQE010000000
VERSION     DAKSQE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.115"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..559
                     /locus_tag="O0V33_002236"
     CDS             <1..559
                     /locus_tag="O0V33_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCX8976851.1"
                     /translation="NIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNRKKILF
                     REVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASLSKKAR
                     YNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHHLEHLD
                     RIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(750..2486)
                     /locus_tag="O0V33_002237"
     CDS             complement(750..2486)
                     /locus_tag="O0V33_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HCX8976852.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     IKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHRELIAKQ
                     GAYEHLYSIQNL"
     gene            complement(2777..3319)
                     /locus_tag="O0V33_002238"
     CDS             complement(2777..3319)
                     /locus_tag="O0V33_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCX8976853.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWKDRYKKIRQY"
     gene            complement(3622..4659)
                     /gene="mutY"
                     /locus_tag="O0V33_002239"
     CDS             complement(3622..4659)
                     /gene="mutY"
                     /locus_tag="O0V33_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCX8976854.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVHDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFKSEHARREMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAVSGTINIERLPDDMIWFDLSDRDQYTFPV
                     PMSKIYQFING"
     gene            4811..5788
                     /locus_tag="O0V33_002240"
     CDS             4811..5788
                     /locus_tag="O0V33_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCX8976855.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTHPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVVGLHPLAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQKLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTKDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            complement(6049..6885)
                     /locus_tag="O0V33_002241"
     CDS             complement(6049..6885)
                     /locus_tag="O0V33_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976856.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRLIALIVFAVLMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGICVLCFLVLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSKDSTDEADT
                     SS"
     gene            complement(6894..>7124)
                     /locus_tag="O0V33_002242"
     CDS             complement(6894..>7124)
                     /locus_tag="O0V33_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8976857.1"
                     /translation="TLVPDDDNRFVASVTQQPIQLLFNDNNQLYGFVYPIVDKKELKD
                     KFNINNNIWITKVGNGYCIANLKEDKWIYIEL"
BASE COUNT         2432 a         1251 c          976 g         2465 t
ORIGIN      
        1 taatattaaa caagatagtc ataatttaaa acaacgtgtc gaaaagttag agaaaattta
       61 tgattactat agcgaagaac gtcctattac aaaaaaacat attcaccaac agaatagaaa
      121 gaaaatactt ttcagagaag tagttcagac gactagacaa gcttatgaag tattaaacaa
      181 attgtcgcgt tatcaaaacg acttatattt acttaataac aatttcctat tacaaatcaa
      241 attagattta gattcattaa ctgcttttca tgagcaaatt ttagctagcc tttctaaaaa
      301 agcacgttat aatgtgacac atgttgatta tgagttagat aatccgcaga aaaaagattt
      361 attgtctaca tttcagcatg agttaataaa tcacccttat caaacggaat attcgtttgc
      421 caatgttatg caaattgttg cagccatcga agagtatcga caccatttag agcatttaga
      481 ccgcataaga attagctttt tcacatatca tagatctgat gcagacattg aaattgttga
      541 agaagacttt gatttataac attaaaacaa ttcctgtaat taataccaca atgagataaa
      601 gtaatattga atctcgaaat taaatttaaa aaagcagtaa gattattttc aatgaagaaa
      661 atctcttact gctttttcta tgttatgacc tcaccatcct gttacaaatc ttccctacaa
      721 aattgtactg aagtcaaaca actaatactt tataaatttt gaatgctata taaatgctcg
      781 taagcacctt gttttgcaat caattcacga tgcgtacctg tttcaacaat atgtccattt
      841 tcaattacga caattttgtc agcatgtgta atagtggaca agcgatgcgc tacgataagt
      901 gtcgttcgat ctttactcaa cacatctaat gcttcttgaa taatggattc actttctaaa
      961 tcaagtgcac ttgttgcttc atccaggata agaattggcg gattatttaa aaatattcta
     1021 gcaatcgata atctttgttt ttgaccacct gataatttaa cacctcgttc acctacttca
     1081 gtgtcatatc cctgtggcaa gttcatgata aagtcatgtg cattagccat tttcgccgct
     1141 tcaactactt cttcatctgt tgctgttgga cgaccaagta aaatattttc cttaactgta
     1201 tcagaaaata aaatattatc ctgttgcacc aatcctattt gatttcttaa acttcccgtt
     1261 aaaaaatctt taatgttgtg accatctatt aaaatttgcc cagaagttac atcgtaaaat
     1321 ctcggtatta agttaattaa tgttgattta ccaccaccac tcatacctac gaaagcaact
     1381 gtttctcctt tttcaatact caaattaata tcttttaaaa ttggagcttc gttatcgtta
     1441 tattgaaaac taacatgatc aatatcaata cgaccttgtt taatttcaat aggttgagca
     1501 ccaacaccat ttttaatgtc ataatcttcg tcaattaatt ggaatacacg gtccattgaa
     1561 gcaaaacttt gcgttaaagt tgtaaatgat gcgactaaac gacgtaaagg cccgaacaat
     1621 aactccaagt atccaacaaa tgctgcaagt gtacctactg tgattgatcc agaaatagca
     1681 agatatgcac caacaccgat gacaattatt ggtccaatat ctgtaactgt attaattgcg
     1741 gcaaaagaat aggcattcca tcttgtatgt ttcaacgcac gtgttaagaa attagtgttc
     1801 tttttatcaa agtttttcgc ttcattgtct tcaatcgcaa aacttttaac gactgaaata
     1861 ccttgaacac gttcatgcaa gaatccttga acctcagcta atgcttgaga tctttcacgc
     1921 gtcaattttc ttaatcttcc aaagaaaacg tacaccgtta aaatgtaaaa tgggaagata
     1981 aacagtgctg ctaaagtcaa tttcacatct aaaaagaaca ttatggatag tgcaataata
     2041 attgttatac aatctaacca aatattcatt aacccggtta aaatgaaatc ttttgtttgt
     2101 tcaacatcat taatcactct agatattact tgacctactt gattattagc ataaaatctc
     2161 gcacttaaag cttgtaaatg gttgtataac tttttacgta tatcatacaa tattttatta
     2221 cttgtccatt gcgccaaata ttgacgtata aattcaattg gtggtctcac tattacaaaa
     2281 ataaataatg cgataccaat ggcaatagtt aaatgatgaa ctttttcatc agtcgttaat
     2341 gcgtggttat taatcacgcc atctattgca tatttaatta aaagtggtat aagcattggt
     2401 ataccaaact taattatccc aacaataatc gttgcaaaaa tacgatattt gtatggctta
     2461 acaaattgca aatatcgttt aatcatacaa tcccccctaa tctattgccc tatcctattc
     2521 ataagcataa aaatgaatag aggttggata cataatttgt agatgtaaat tcttcttaca
     2581 atttacattt ttaaaattaa gatatctcaa cctctgtcat attcatgtga ttaaatacgt
     2641 tgtcttattc gtatgttata ttctattcaa tttaatctat ggatactgtg tccccacacg
     2701 acagcaaaag ttatcatact tctttacatc actaagtcaa tataaatgat ttaatcagta
     2761 tttacacttt atttgcttaa tattgtctaa tttttttgta acggtctttc caaactttga
     2821 taaaatctgg cgcaaatggg cccttctttt gttctatcca ttgttgaaga atgtccacgt
     2881 tgcgtcttaa aataatatca atatcatgcg gataattcat ttgattcata tgttgctcat
     2941 attcatcttc atctaataaa tgatactttc catttggata tactttaata tctaaatcat
     3001 agtctatata ttttaatgcc tcttcatcac aaacaaatgg cgatgacaaa ttgcaatagt
     3061 aataaattcc atcttctcta aacatgcaga taacattaaa ccaatattct gagtgaaagt
     3121 aaacaattgc cggttcacgt gttatccaag ttcttccgtc actttcagtt actaacgtat
     3181 gatcattgcc accaatgaca acatgatcag taccctttaa tattgttgtt tcagaccaaa
     3241 cgcgatgaat cttaccatca tgtttataac tctgaatttt aatgttttcc ccttctttag
     3301 gtatggattc tctgaccata ctccacacca ccttctgtta atttaaccat tataaattat
     3361 agcatatttc agaaatagta ttatataaat acatattttt acgaaataag attttactac
     3421 ttaatgatta aactcggtaa tattgctaag tactacaaca gagatttaca tgtcccattt
     3481 aaagtatata aaatcatcac ttttatatat caacacttta actttttgac attgttatcc
     3541 tctgagaatt gaagataaca tttatacatt tttaaaatta atgtcactat attttccgat
     3601 aatcttatca atcatcgaat gttacccatt tataaattga taaatctttg acataggtac
     3661 agggaatgta tattgatctc gatcacttaa atcaaaccaa atcatgtcat ctggtaatct
     3721 ttcaatgtta attgttcctg aaacggcgta tacctttatc ttccatgtta aatgagtaaa
     3781 ttgatgcttc aattcaaaaa taggtgtttc tactggttga atgtcatgac cgattttttc
     3841 agtcatttca cgtctagcat gctcactttt aaacatagga aattgccaca taccatgcaa
     3901 taatttttcg ctacgctttt gcaacagata ttgaccttga ttatttctaa ttaaaaagac
     3961 ggattgctca attacttttt tacttacatt tttagattta actggtaact tttcaaatgt
     4021 acctttatca aatgcctcac agttttcttg aactggacaa aataagcata atggattttt
     4081 cggtgtacaa attaatgccc ctaattccat catagcttga ttaaacgttc cagcttctgt
     4141 agtaacatac ggtaataatt cttgttcgta cgatttcctc gtcgattgta atttaatatc
     4201 tcgatagtca tcattcaatc tagaccatac ccgaaaaaca tttccgtcta cagttgctag
     4261 tggtacatta tatgcaatgc tcattactgc agcttgtgtg tatgggccaa caccttttaa
     4321 agctttaaat tgatcaggat ctttgggaac taagccttca tatttatcat gaacttcctt
     4381 aattgccgta tgaaaatttc gagctctact ataatatcct aagccttccc agtactttaa
     4441 cacttcatct tccgaagctt gactcaaaac ttccacagtt ggaaatcgtt caacaaaacg
     4501 atgataatag tcaataactg ttttaacttg tgtctgttgt aacatgactt cacttaacca
     4561 aatatagtac ggattggtcg tttgtcgcca tggcatttct ctttgatttt catcaaacca
     4621 gtgtatcaaa ttttctttaa aactagactg ctgatacatt tataaaaccc tttcctcacc
     4681 aaaattaatt gtctttactc ataatgtttt tattgtacat taaaatcatg gttagtatgt
     4741 aagttaattt agttatttgc gaaattggat tataatagta tatataatat tatgaaatga
     4801 gtgaactgat atggacactg caacacatat cgcaattggg gtgggcctta cagcacttgc
     4861 aactcaagat ccagcaatgg cttctacgtt tggtgcaaca gctacaaccc ttatcgttgg
     4921 ttcattaatt cctgatgggg atactgttct taaattaaag gacaatgcaa catatatttc
     4981 gcatcataga ggtatcacgc attccatccc tttcacaata ctatggccaa ttttaattac
     5041 atttttaata ttcacgttct ttagtggaac ccacccattt catgtatgga tgtgggctca
     5101 gctcgcagta tttttacatg tctttgtaga tatattcaat tcttatggta cacaagctct
     5161 tagacctatc acaaacaaat ggattcaatt aagtgtgatt aacacatttg accctattat
     5221 tttcacagtt ctttgtattg gtattgtatt atgggttgta ggcttgcatc cacttgcagt
     5281 cttctttcct ataatcgctt tactaatcat ttattacatg attcgtttta aaatgagagc
     5341 cgtaattaag caacaagctt taaaagcaat tcaacaagag catcaccctg ttaaagtatt
     5401 tgttgcgcca acaataaaat ttatggaatg gcgtgtcgcg atacaaactg atgcacatga
     5461 ctatgttgga aaagcatatg gtagaaatgt ggtgtttagt gataaagttg aacgtcaaaa
     5521 attatcaaca gactccattt tatggaaagt caaaggtaat aaagatatac gtactttttt
     5581 aaacttttca tcaatctatc gttggcaaac aacaacgtta gcagatggtt ctactgaaat
     5641 tcgtttgatt gatttgcgtt atttaaaaaa tgatcattat tcatttgtgg caattgcaca
     5701 cgtaacaaaa gataatgtca tagaccactc ttatattggc tgggtattta cagaagataa
     5761 gttacaacgt aaactgtatg ctaaataatt tcaagttatt attcactaaa gttaatctat
     5821 aaaaaatgaa caaccgggca gaatgaaaat caaaacgatt tttactctgt ccggtttttt
     5881 aatgtaaaac tatgaatgct tttacaaaac ctaatattta tattgtcgct aacaaactac
     5941 cttcaatgac tcgaaatatt aaaatcagta taggaaaaca atatctagat tatattctaa
     6001 ttatttctga ttctcacaga ttaatttaca caacaagtca gctaaacatc atgaagaagt
     6061 atccgcctcg tctgtactat cttttgaaac atcttgttga ttatcagttt gtgtcacttt
     6121 agatgtttta ttataaattg cgtgtgtcgt ataccttgct aatacgaaat ttactgcagc
     6181 tattaaacat aagaataaaa taaagtaaaa atgatatgga atgttttcca tacttgatat
     6241 accaaatatg atagattgtg ctataccatt aacaatgtaa tacattggat tcagcattag
     6301 gatgtgattg ataaatacat gatttggatt tggtataaaa ataattggca acactaagaa
     6361 acacaataca caaatcccat aaaatatgat atttattttt tcagttaaca gtcgaataag
     6421 accaaaagta actgatatta atcctacaaa aatagttgcc atcacaataa aatagaatag
     6481 cgctatatat gatgtttcga agtttactgg tttaaccaat gcactaatca tcgtcaaaat
     6541 gactagcata ataaaactta aaatagacat aataactact ggcgtcgctg cggaaatatt
     6601 aaataactta gtaactaaaa agtcattcat ataatgatga taagagatat aaattgtgaa
     6661 catcaatacc gcaaaaacaa tcaatgcaat aagtctaaat aaccatcgca cctctttaac
     6721 atcggtcaaa tgactaaagg aaaatagcgt ttcacttgca ataataagta ttaaacatgc
     6781 agctaatacg attagaaaac ttttccaact ttgttttaaa cgttctgtta catacctttt
     6841 agtatgcggt aagtttttaa aatataaaat aatattatct agcatcttta cacctacaat
     6901 tcaatataaa tccatttgtc ttcttttaaa ttggcaatac aatatccatt cccaacttta
     6961 gtaatccaaa tgttattgtt aatattaaac ttatctttta attctttttt atctacgatt
     7021 ggataaacaa aaccgtataa ctgattatta tcattgaaaa gtaattgtat cggttgttgt
     7081 gtaacggatg caacgaaacg gttatcatca tcaggaacta aagt
//
LOCUS       DAKSQE010000116         7122 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.116, whole genome shotgun sequence.
ACCESSION   DAKSQE010000116 DAKSQE010000000
VERSION     DAKSQE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.116"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            14..892
                     /locus_tag="O0V33_002243"
     CDS             14..892
                     /locus_tag="O0V33_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HCX8976858.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESE
                     IKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITD
                     INQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDIT
                     HGDKAFEKKLEKLEEK"
     gene            1046..1366
                     /gene="ytxJ"
                     /locus_tag="O0V33_002244"
     CDS             1046..1366
                     /gene="ytxJ"
                     /locus_tag="O0V33_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HCX8976859.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            1791..2915
                     /locus_tag="O0V33_002245"
     CDS             1791..2915
                     /locus_tag="O0V33_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCX8976860.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAHQLNHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPESYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(3103..4329)
                     /gene="pepT"
                     /locus_tag="O0V33_002246"
     CDS             complement(3103..4329)
                     /gene="pepT"
                     /locus_tag="O0V33_002246"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCX8976861.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDADVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDTAENN"
     gene            complement(4343..4837)
                     /locus_tag="O0V33_002247"
     CDS             complement(4343..4837)
                     /locus_tag="O0V33_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCX8976862.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRSYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            complement(4855..5616)
                     /locus_tag="O0V33_002248"
     CDS             complement(4855..5616)
                     /locus_tag="O0V33_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCX8976863.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            complement(5812..6882)
                     /locus_tag="O0V33_002249"
     CDS             complement(5812..6882)
                     /locus_tag="O0V33_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCX8976864.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
BASE COUNT         2560 a         1033 c         1234 g         2295 t
ORIGIN      
        1 aaggagtgca atcatgaaaa aaattgttat tatcgctgtt ttagcgattt tatttgtagt
       61 aataagtgct tgtggtaata aagaaaaaga ggcacaacat caatttacta agcaatttaa
      121 agatgttgag caaaaacaaa aagaattaca acatgtcatg gataatatac atttgaaaga
      181 aattgatcat ctaagtaaaa ctgatacaac tgataaaaat agtaaagaat ttaaggcact
      241 acaagaagat gttaaaaacc atctcatacc taaatttgaa gcatattata agtcagcaaa
      301 aaatttgcct gatgatacaa tgaaagttaa gaaattaaaa aaagaatata tgacgcttgc
      361 aaatgagaag aaggatgcga tatatcaatt aaaaaaattc ataggtttat gtaatcaatc
      421 tatcaagtat aacgaagaca ttttagatta tacgaaacaa tttgaaaaaa atagatacaa
      481 agttgaatca gaaattaaat tagctgataa taaaagtgaa gcaactaatc ttacgacaaa
      541 attagaacat aataataaag cgttaagaga tactgcgaaa aagaacctag atgatagtaa
      601 agaaaatgaa gtaaaaggcg cgattaaaaa tcacattatg ccaatgattg aaaaacaaat
      661 taccgatatt aaccaaacta atattagtga taagcatgtt aataatgcaa ggaaaaacgc
      721 aatagaaatg tattacagtc tgcagaacta ttataataca cgtattgaaa caataaaggt
      781 tagtgagaag ttatcaaaag tcgatgtaga taagttgccg aaaaagggta tagatataac
      841 tcacggcgat aaagcctttg aaaaaaagct tgaaaaatta gaagaaaaat aactataatc
      901 atttttcaaa gttaaaaatt ttgaatttat ggttaacatg tcaacttact atgtgtataa
      961 tggtaaacat tgatattaac tatatgtata aaaatgtcac gcagatgcta tttaaatgtg
     1021 ataaatattt ttagaggtga atagagtggc tataaagcta agttcaattg accaatttga
     1081 acaggttatt gaggaaaata aatatgtttt tgtattaaaa catagtgaaa cttgtccaat
     1141 atcggcaaat gcgtacgatc aatttaataa atttttatat gaacgcgata tggacggtta
     1201 ttatttgatt gtccaacaag aacgcgattt gtcagattat attgctaaaa aaacgaacgt
     1261 taaacatgaa tcacctcaag cattttattt tgtaaatggt gaaatggttt ggaatcgaga
     1321 ccacggtgat attaatgtgt cgtcattagc acaagcagaa gaataatgaa actatagggt
     1381 tggaacattt tgccttacac tactagacgt gaatagcaca acttaaattc gtgtgaatca
     1441 gagtagtttg gctataatga tgttctgacc ttttatttta tgtaaccttt agaagcagtt
     1501 aagttagtac ttttttacaa acatatgtat aatgtattcg agtattttta ttgaaaatat
     1561 tttggaaaac gacgaatcca ataagaaaat tttaacatga tttgtaagtt agtttaatag
     1621 gaaatacatg ctaaaccata agaagtatac tgttatttgc tggaataatt aataatcatg
     1681 tcatgttaaa tgttcgcata taatcacgag ataaaatcta aaatttaaga ttaatctttt
     1741 atgaataaaa aacgtatcac aacaaataat aaagtaaggt ggtcaaggtt atgaaagtat
     1801 tagtagccat ggatgagttt catggaatta tttcgagtta tcaagctaat agatatgttg
     1861 aagaggcagt tgcaagccaa attgaaactg cagatgtagt tcaagtacca ttgtttaatg
     1921 gaagacatga attattagat tctgtatttt tatggcaatc tgggcaaaag tatcgtatac
     1981 cagtacatga tgcagatatg aatgaagttg aaggtgttta cggacaaact gatacaggga
     2041 tgaccgttat cgaggggaat ttatttttaa aaggtaaaaa accaattgtt gaacgaacaa
     2101 gttatggttt aggagaaatg attaaacatg cattagataa cgacgcaaaa catgttgtaa
     2161 tttcattagg tgggattgat agttttgatg ctggtgcagg tatgttacaa gcattaggtg
     2221 ctcaattcta tgatgacgaa gggcgtgtcg tagatatgag acaaggtgct ggtgtaatta
     2281 aatatattcg tcgtatggat atgtcgaact tacaccctaa aatggaaaca gcaagaattc
     2341 aagtaatgtc ggatttttca agtcgattat atggtaagca aagtgaaatc atgcaaactt
     2401 atgatgcgca tcagttgaac cataatcaag cagcagaaat cgataattta atttggtatt
     2461 ttagtgagtt atttaaaagt gaattgaaaa ttgcaattgg tccagttgaa cgtggtggtg
     2521 ctggtggtgg aattgcagca gtcttgaatg gactgtatca agctgaaata ttaaccagtc
     2581 atgcattagt agaccaacta acacatttag aaaatttagt tgaacaagcg gatttaatta
     2641 tttttggaga aggattaaat gaaaatgatc agttgctaga aacgacaaca ttgcgtattg
     2701 cagaactttg tcataaacat caaaaggttg ccattgcaat ttgtgcaact gctgaaaagt
     2761 ttgatttatt tgaatcacaa ggggttacag caatgtttaa tacatttatc gatatgccgg
     2821 aaagttatac cgactttaaa atgggattac aaattaggca ttatactgtt cagtctttaa
     2881 aactgttgaa aacacatttt aatgttgagg tttagtaaag aaggactaaa ttggtgatgc
     2941 tgtcatgatg gttaataaca tttatgatgg ttagcaaaac gaattagaag atcgaaagta
     3001 tacgtaaaaa atatgaaaaa tcacgctatc attgcactga atgttagcgt gattttttat
     3061 attaattaag cctgagttga actagtatat aatcgtttgt ttttagttat tttcagcggt
     3121 atcttctaca attccaatga ttacttgtac tgctttttcc atgacatcaa tggatgcata
     3181 ttcatatggg ccgtggaagt taccgcaacc tgtaaagatg tttggagttg gtaatcccat
     3241 aaatgacaat tgtgaaccat ctgtaccacc acgaataggt tcagtgtttg ctggaatatc
     3301 taatttagca aagacacgtt taggtatatc aataatatga ggcaatggta atattttttc
     3361 tgccatattg aaatattgat ccgatatatc aactttaact ggataatttt caaaatgggc
     3421 attgatatcg tcacgtattt ctaaaatacg tttcttacgc aattcgaatt gttttttatc
     3481 atgatcacga ataatgtatt gcaaagttgc tttttcaaca gttccttcaa agttcattaa
     3541 gtggtaaaag ccttcatatc cttctgttcg ctccggaact tcactatcag gtagcaaact
     3601 atcgaattgt tcacctaaac gtattgcgtt taccattgca tttttagctg aaccaggatg
     3661 aacattcaca ccgtggcatg taataaccgc ttcagcagcg ttaaagcttt catattgtaa
     3721 ttctccatat tgactaccat ccatagtata agcaaaatca gcattgaagc ggtcaacatc
     3781 aaatttatgt ggaccacgac cgatttcttc gtctggtgta aatccaatgc gaatggtacc
     3841 atgtttaatt tctggatgtt cttgtaaata acaaatagct tccataattt ccacaatacc
     3901 cgctttatcg tctgcaccta gtaacgatgt accatcagtt accattaatg tatgaccaac
     3961 taaactgtta agttctggaa atactttagg atctaagaca cgtttagtat tgcctagttt
     4021 gtatggcttg ccatcatagt tttcaataat ttgcggttta acatttgaag cattgaaatc
     4081 aggtgatgta tcaacatgag ccaaaaaacc aacagttggg acgtcggcat cgatgttact
     4141 ttctaatgta gcaaataagt agccattttc atctaaatca gttggcaatc ctaattgttg
     4201 taattctttt tctaataaat gtaacaaatc ccattgcttt tcagttgaag gtgttgttgt
     4261 agattttgga tcagattgcg tatcaattgt cgtatatctt gttaatctat ctatcaattg
     4321 gttcttcatt atattcgacc ccttaaactc tattattcat gttgtaagat tttttatatg
     4381 tcttaccttt gattttacca tacagttgtt tgatacgtgt gtacaggtaa tatagaattt
     4441 cagaaactaa tataccgaaa gcaatcgcac ctgaaatcag tgtaacttct aaaaatgtat
     4501 ttacagcact tgtataatca ttcgatacta aaaaacgagt cgcttgataa gctgcaccac
     4561 caggtactaa tggtataatg cctggcacta tgaatataat taccggtcgt ttataactgc
     4621 gactcatagt atgactcatt aagcctaaaa ttaagcttcc caaaaatgaa gcgccaactt
     4681 ttccaaactc taaatctacc gttaattggt aaatcgtcca tgcaatggca cctacaaatc
     4741 cacatgctac taagagacgt ttgggtgcat tgaaaatgat agagaaaagt actgttgata
     4801 taaagctgat tgtaaaatga aataaataaa atagcatgct ttaacagtcc ttccttaaat
     4861 gattaataaa acgattgcga caccagcacc gattgcgaat gctgttaatg cagcttcaac
     4921 accacgagac atacctgcaa gtaattcacc cgctaataaa tctcgaatgg cattggtaat
     4981 taatatacca gggacaagtg gcatgacact ggctatagta atgatatctt gattggttgc
     5041 aatacctaat ttagtaaatg tggctgcaat ggatatgacc acagcggctg caacaaactc
     5101 tgagaaaaat ttaatttgta tatagcgttg cacaaagctg aatgttaaaa atgcggatcc
     5161 accagcaatg actgcaatcc aacaatctga tgcgacacca ccaaacatga acaggaagaa
     5221 gccacatgca atggcagctg caaagaaatt cgttaaaaaa gaatattgta atgatgcatg
     5281 ctgtaaatga ataaattcag atttagcttc atcaattgtg agttctttat ttgatatttt
     5341 acgtgaaaga ctattcgtta aagcgatttt ctctaaatct gttgtacgct cttgtacacg
     5401 aattaatctt gtacttgtac gatcatttaa tgaaaaaata attgcagttg aactgacaaa
     5461 actatatgta ttatgaagac cataactatg tgcgatacgg ttcattgtat cttcaactcg
     5521 atatgtttca gcacctgatt caagtaaaat tctacctgca attaatacaa catcaatcac
     5581 tttgttttca tctataattg tgattgaatc tggcatatca attcacctcc aatgatatgt
     5641 gttatttatt tgaacaattg aagtttacaa cttgttgttg caactttcaa tagtgagaat
     5701 ttgtgttagt atgatgaact tgtatggttc aaatttaaat aagaaaaact gttaatcttt
     5761 gctattatac tatgatttaa taatagcaaa ggattaacag ttttgtcgtt gttatagatt
     5821 gatgataggg ttaaacatta ctttatttcg gccttgattt ttggctacat gcaccatatc
     5881 gtctgcatct ttaaacactt tacgctgtga ttttggatcg tcatctgtta aataaccaac
     5941 accgatagac actgacaatt taataacttc tttgtttggt aaatggaatg atgatttttc
     6001 aacacccgaa cgaatatttt cagctaattt aacactttga tcaagtgaat agttgtgaat
     6061 gacaactgag aactcttcgc caccatttct aaaaatttta aattgattcg gcacatagtt
     6121 tttaagtaat tgagacattt gttttaatac agcatcacct gatttgtgtg agtaggtatc
     6181 attgacatct ttaaatccat cgatatcgat taataataat gcgatacttt gatgttcttt
     6241 ttcagctttt cgtgaaattt catttaaatg tctatcaaat tcttttacat tacctaagcc
     6301 tgttaagtaa tcatatttat cttcgttttc ataacgattt acgagtgaga agaaatgcca
     6361 aatatcgaca aatgttatcg ctgaagctaa agtgataatt aatgaaattg gtattaaaat
     6421 gataacttcc gatagtgtgt aaataggact cactaatgcg acaccaaata aaatgattat
     6481 tgtaacaaca ttaagtatta acaatgatag cacatcattt tgttttaaaa atggtccaat
     6541 agcacttgtt actgcggcaa taacaatcaa cgtaacaccg tacataatcg agttgttaaa
     6601 tactacaatt tcaacaattg ctacaattac tgtggcagat aatgtataga ccatatttgt
     6661 aaatctacct aaaaacaata aaggaacgaa tgttaagtga attaaataat cttcacgata
     6721 agggataggg tagacagata ataataatga tacgattgtc attaatacag tgacatatgc
     6781 cttagaaaaa accatacgtt tgttttctga atactgtaag cgatggaata aatagattcc
     6841 agcgactata acagatatat tgtatataaa tgcttcgaac atgtctgaat cgactccttt
     6901 aattgaccac tagctattgt aagtgaaaac ttacaatttg tcattagttt acatataaaa
     6961 ttaatgtatg atatagactt tgatgttaaa atgttgcctt aaatgatatg atgaaaaaat
     7021 gaataatagg cgatatataa gaaatgaatc gtatagttgt aaatatgata tcattgattg
     7081 agcgaattaa tttataataa agctataaga tatacgtaga aa
//
LOCUS       DAKSQE010000117         7050 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.117, whole genome shotgun sequence.
ACCESSION   DAKSQE010000117 DAKSQE010000000
VERSION     DAKSQE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7050
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.117"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            243..1226
                     /locus_tag="O0V33_002250"
     CDS             243..1226
                     /locus_tag="O0V33_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCX8976865.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEGGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
     gene            1417..2343
                     /gene="pstC"
                     /locus_tag="O0V33_002251"
     CDS             1417..2343
                     /gene="pstC"
                     /locus_tag="O0V33_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCX8976866.1"
                     /translation="MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            2345..3262
                     /gene="pstA"
                     /locus_tag="O0V33_002252"
     CDS             2345..3262
                     /gene="pstA"
                     /locus_tag="O0V33_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCX8976867.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKYSKKF"
     gene            3309..4160
                     /locus_tag="O0V33_002253"
     CDS             3309..4160
                     /locus_tag="O0V33_002253"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCX8976868.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPTVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            4167..4808
                     /gene="phoU"
                     /locus_tag="O0V33_002254"
     CDS             4167..4808
                     /gene="phoU"
                     /locus_tag="O0V33_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCX8976869.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYNINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
     gene            complement(4948..6762)
                     /gene="pepF"
                     /locus_tag="O0V33_002255"
     CDS             complement(4948..6762)
                     /gene="pepF"
                     /locus_tag="O0V33_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCX8976870.1"
                     /translation="MSQGLPLREDVPVSETWDLTDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSQLSFVESEILELPEEILQQLEKSCPYQHYIKQLIKQKPFQLSASVEQLLAILS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTLNEMLMANYLFNKSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYRKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
BASE COUNT         2470 a         1102 c         1191 g         2287 t
ORIGIN      
        1 ttttaatttc atctgtccat tttttgtgtt catatttaaa tttggaaaat ttatcaggaa
       61 acatttcaat tttactgatg aagcaatatt tttcagatta acaaaaaata atatttacat
      121 tttcttaaca aacatttatg taacatttac agtttctaaa gatgaggtta ataattcgag
      181 gttaagataa agatgtaatc aatacaaata ctatttgttg ttcatacagg gaggatattt
      241 caatgaaaaa atggcaattt gttggtacta cagctttagg tgcaacacta ttattaggtg
      301 cttgtggtgg cggtaatggt ggcagtggta atagtgattt aaaaggggaa gctaaagggg
      361 atggctcatc aacagtagca ccaattgtgg agaaattaaa tgaaaaatgg gctcaagatc
      421 actcggatgc taaaatctca gcaggacaag ctggtacagg tgctggtttc caaaaattca
      481 ttgcaggaga tatcgacttc gctgatgctt ctagaccaat taaagatgaa gagaagcaaa
      541 aattacaaga taagaatatc aaatacaaag aattcaaaat tgcgcaagat ggtgtaacgg
      601 ttgctgtaaa taaagaaaat gattttgtag atgaattaga caaacagcaa ttaaaagcaa
      661 tttattctgg aaaagctaaa acatggaaag atgttaatag taaatggcca gataaaaaaa
      721 taaatgctgt atcaccaaac tcaagtcatg gtacttatga cttctttgaa aatgaagtaa
      781 tgaataaaga agatattaaa gcagaaaaaa atgctgatac aaatgctatc gtttcttctg
      841 taacgaaaaa caaagaggga atcggatact ttggatataa cttctacgta caaaataaag
      901 ataaattaaa agaagttaaa atcaaagatg aaaatggtaa agcaacagag cctacgaaaa
      961 aaacaattca agataactct tatgcattaa gtagaccatt attcatttat gtaaatgaaa
     1021 aagcattgaa agataataaa gtaatgtcag aatttatcaa attcgtctta gaagataaag
     1081 gtaaagcagc tgaagaaggt ggatatgtag cagcaccaga gaaaacatac aaatcacaat
     1141 tagatgattt aaaagcattt attgataaaa atcaaaaatc agacgacaag aaatctgatg
     1201 ataaaaagtc tgaagacaag aaataataag acgcaatttc aaatgtgtct tgaaacatga
     1261 ttttgatggt gaatcattat ttagagtgca aagcttgatt tatcgagacg ctgattttga
     1321 cattcagtta gtctacaagc ttatcaactt aaaatagtgg ttcatcatta ttttacaaat
     1381 ctaattattt tgggagtaat agaaagaggt ttgattatga cttcatctac taatgttaaa
     1441 gctttaattg aaaaaaataa taataaaaaa gggaagcata atgacaaaat tataccagtt
     1501 attttagctg caatttcagc gatttccatt ttaacaacac taggtatatt aatcacattg
     1561 cttttagaaa ccatcacttt tttcaccaga attccaataa ctgaatttct attttctact
     1621 acttggaatc ctaccggctc agaccctaag tttggtatct gggcattgat aatagggact
     1681 ttaaaaatca cagttattgc gactatattt gcagttccag tcgggcttgg tgcagcaatt
     1741 tatttaagtg aatatgcaag tgaccgtgca cgacgcataa taaaaccgat attagaaatt
     1801 ttagcaggaa taccaacaat tgtgtttggt ttctttgcat taacctttgt tacaccagta
     1861 ttaagatctt tcatacctgg tcttggagag tttaatgcta taagtcccgg cttagttgta
     1921 ggtattatga ttgtccctct catcacaagt ttgagtgagg atgcaatggc atctgtacca
     1981 aataaaattc gagaaggtgc ctatggactt ggagcaacta aattagaagt agcaactaag
     2041 gtcgtacttc ctgcagcaac atcaggtatt gtagcttcaa tagttctcgc gatttcaaga
     2101 gcaattggag aaacgatgat tgtatcatta gcggcaggta gttcaccaac agcttcatta
     2161 agtttaacaa gttcaattca aacaatgact ggatatattg ttgagatagc gacaggtgat
     2221 gcaacatttg gatcaaatat ttattacagt atttatgctg taggattcac attatttatc
     2281 tttaccttaa tcatgaattt actttctcaa tggatttcta agcgttttag ggaggagtat
     2341 taatatggaa acgacagata ataatagaca atcactcgtc gatcaacaac ttgtccaaaa
     2401 acatttatca tccagaacgg ttaaaaataa agtgttcaaa ctcatatttt tagcatgtac
     2461 attattagga cttgtcgtac ttattgcgtt gttaactcaa acattgatta aaggggtaag
     2521 tcatttaaat ttacagtttt tcactaattt ttcttcttca acaccatcta tggctggcgt
     2581 taaaggcgca ttaatcggtt cactttggtt aatgttaagt atcattccat tatcaatcat
     2641 cctaggaata ggtacagcta tatacttaga agaatatgcg aaaaacaaca aatttactca
     2701 gtttgttaaa atcagtattt ccaatttagc tggtgtacca tcagttgtat ttgggttatt
     2761 aggttacact ttgttcgttg gtggtgccgg gattgaagcc ttgaaaatgg gtaacagtat
     2821 attggcagca gcgctaacaa tgaccttact gatattacca attattattg tttcaagtca
     2881 ggaagcaatt agagctgtac ctaactcagt acgcgaagct tcttacggct taggtgctaa
     2941 taaatggcaa acgataagac gtgttgtctt accagcagcg ttacctggta ttttaactgg
     3001 attcattttg tctctttcaa gagcactggg agaaacagcg ccacttgtgc ttatcggtat
     3061 accaactata ttattggcaa cacctagaag tatattggat caattttcag cattacctat
     3121 ccaaatattt acttgggcga aaatgccaca agaagaattc cagaatgttg catcggcagg
     3181 cattatcgtt ttactagtta tcttaatctt aatgaatggc gttgcgatta ttttacgtaa
     3241 caaatatagt aaaaaattct aatttaaaca atcaatctaa tttatctatt aaaaagggag
     3301 tattaaatat ggcgcaaaca cttgcacaaa ctaaacaaat atctcaaagt catacgtttg
     3361 atgtctcaca aagtcatcat aaaacaccag atgatacaaa ctcacattct gttatatatt
     3421 caacacaaaa tttagactta tggtatggcg aaaatcatgc attgcaaaat attaatttag
     3481 atatttatga aaaccaaatt actgccatta taggtccatc tggttgtggc aaatcaacat
     3541 atattaaaac tttgaatcga atggttgagt tagtacccac agtcaaaaca gctggtaaaa
     3601 tactatatcg cgatcaagac atttttgatc aaaaatattc taaagaacaa ttacgtacaa
     3661 atgtgggcat ggtcttccaa caacctaatc catttccaaa atcaatatac gataatatta
     3721 cttacgggcc aaagattcac ggtattaaaa ataaaaaagt tcttgatgaa atcgttgaga
     3781 aatcattacg tggcgctgca atttgggatg aattaaagga taggttgcac acaaatgcat
     3841 atagtttatc cggtgggcaa caacagcgtg tttgtatcgc gcgttgttta gcaattgaac
     3901 ctgaagtcat tttaatggat gaacccacat cagcattaga tccaatctca acattaagag
     3961 tagaagagtt ggttcaagaa ctaaaagaaa agtacacaat tattatggtt acacataata
     4021 tgcaacaagc agctcgtgta tcagataaaa ctgcattttt cttaaatggt tatgtcaatg
     4081 aatatgatga cactgataaa attttctcta acccatcaaa caagaaaaca gaagattata
     4141 tttcaggaag gtttggttga tatataatgg caataattag acaacgatat caggagcaac
     4201 ttgatgattt aataaaagaa ttacgtcggt taggtgcaaa tgtctatgtg agtattgaaa
     4261 atggtataaa atcattaagt attgacgata gaggctttgc acgacaaaca gttaaaaacg
     4321 ataaacatat caatcaatta aattataata ttaatgagcg agttatcatg ttaattacaa
     4381 agcaacagcc cattgcgagt gatttgcgta tgatgatttc ttcattaaaa atcgcctccg
     4441 atttagaaag aataggagat aatgcatcga gtattgccaa tattcgattg cgtacgaaga
     4501 ttacagatga ttatgtgtta acccgtttaa agacaatggg taaactagct atgttaatgt
     4561 taaaggattt agatcaagca tttaaaaaga aagataccgt attaataaga gaaataattg
     4621 agcgtgatga agatatcgat gacttatata gtcatattat taacgcaacg tatcttattg
     4681 ataacgatcc atttgtcgct gcacaagccc atttagcagc aagacatcta gaacgtattg
     4741 gtgatcatat tattaacatc gctgaaagtg tttattttta tttaacaggt acacattacg
     4801 aacaataact taaagttatt actataaaat cccttacgat aaatatatat ttctattatt
     4861 cataaaccct caaaaaaacc aagattctca caattagtaa tgtgaaaatc ttggtttata
     4921 ttgttctact ataagttgtc tcgcatccta gttatttgct tgctcaattt catctgttaa
     4981 tttttcaact tcatccacta aatcagaaat atattgaatt gtagatttaa gtggctgttc
     5041 tgtagtaatg tctacacccg caatgtttgc aagttcgaca ggtgatacac taccaccttt
     5101 tttcaatgtt tctaaccaag catcaacagc tggttggcct tcatttttaa tcttttgaga
     5161 aacgacagtc ccgattgtta agccagcaga atacgtatac gaatataatc ccatatagta
     5221 atgaggttga cgcatccatg ttaattcagc accctcagtc atgtctactg catctccgaa
     5281 aaattgttta taaacattta gcattatttc atttaatgtc ggcgcattta aagattcacc
     5341 ttgatctact ttgcgataca cttcacgttg ataagcagct tctaataagt gggtaaccat
     5401 attatgataa tatgttctag ataaaattga gccaataacc caacgcttaa atcttggatt
     5461 atcacttttg ttaaataaat aattggccat caacatttca ttcaatgtag aaggggcttc
     5521 aacaaagtac attgatgctt ctgattcaag atatggttga tgtttttgag ctaatgtaaa
     5581 atgacctgca tgacctaatt catgtgctaa gacaaatgct tcagccattt taccagtcca
     5641 agaaataaac acatatgaat gtgtaaagta tggacttgca caaaatgcgc ctgtatcttt
     5701 acctttattt tgtgcaaaat caatccatcg ctgatcgtat gcttcacgta acatgtttgt
     5761 atagtcatca cctaaaacac ttaacgcacc gaaaatataa ttttttgagt cttcaattga
     5821 aatctctggt tcataatcag ggtctacaga aatttttaag tcttcaaaac gcatgttatc
     5881 taatccgtga atacgttgta aaagtttagc atatttctgc ataactggtg ccaagtcacg
     5941 cataatcata tcgatttgac ggtcaaacat atcacgcgtt acttcttgac tatgtaataa
     6001 ataatcgatg accgattcaa acccacgtaa atcagcttca attttttctt gttgtacttg
     6061 catattatat gtagccgcgg tagtatgctg atattttcga atcccatcgc taaacgattt
     6121 gaaactttta cgtctaaact caggatcttt attatcttca taatcatttt caaacgtagc
     6181 atagtcgaca gggtacgttg taccatcatg ttcaaatgaa tcgaatgtaa tatctagcat
     6241 ttttgtcgtg ccgtataaat cgtaaggact gtttagcgta ggtgataaaa ttgctaataa
     6301 ttgttctacc gacgcagata attggaatgg cttttgtttt attaactgtt taatatagtg
     6361 ttgatatgga catgattttt ctaattgttg aagtatttct tctggtaatt caagtatttc
     6421 tgactctaca aatgataatt ggctaacaat tttaccgtat gtagtagata atttagcgct
     6481 caatacttgt gcctcaatat tactagtatc tacacttaaa cgtagttctg cataattact
     6541 taagcgatct aaggcaatta aaatattttc taattcagct aaagcagtat taatttgttc
     6601 gattgaattt aatgttgttg catatgtatg atgaaattga tttgcttgtt gtactagagc
     6661 gtcaatactt tcataatatt gttgatcatc tttaaataag tctgttaaat cccatgtttc
     6721 tgaaacagga acatcttctc ttaaaggtaa accttgagac atataatcac tcctttggta
     6781 taatacattc attatacata attttgtctc ctaaacgata atgttacgat tcccatgtcg
     6841 caaggaacaa tgatttttct aatagtagat agaatattgc ttgaaactga ctgctattgt
     6901 ttaagttttt aaaaatttat aataaaatgt atacaaggaa agtaatgaaa taaaaggaga
     6961 atgaatatga cattattcga tatgcctaat tatttatgga tcacaacttt aataatgatt
     7021 ttattaacaa tattctgttg cttagtttta
//
LOCUS       DAKSQE010000118         7034 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.118, whole genome shotgun sequence.
ACCESSION   DAKSQE010000118 DAKSQE010000000
VERSION     DAKSQE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7034
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.118"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            52..711
                     /gene="arlR"
                     /locus_tag="O0V33_002256"
     CDS             52..711
                     /gene="arlR"
                     /locus_tag="O0V33_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HCX8976871.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            708..2063
                     /gene="arlS"
                     /locus_tag="O0V33_002257"
     CDS             708..2063
                     /gene="arlS"
                     /locus_tag="O0V33_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HCX8976872.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNAYLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIDYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
     gene            2537..5335
                     /locus_tag="O0V33_002258"
     CDS             2537..5335
                     /locus_tag="O0V33_002258"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCX8976873.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGIISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPAELALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            5349..6620
                     /gene="sucB"
                     /locus_tag="O0V33_002259"
     CDS             5349..6620
                     /gene="sucB"
                     /locus_tag="O0V33_002259"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCX8976874.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNLGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAVIGEGSGNASKENSNDNTPQQNDETTNNK
                     KEETTNKSADKAEVNQTNDDNQQRVNATPSARRYARENGVNLAEVSPKTNDVVRKEDI
                     DKKQQAPASTQTTQQAPAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAML
                     TTFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGD
                     DMITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMV
                     NGSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSY
                     DHRIIDGKEAVGFLKTIKELIENPEDLLLES"
BASE COUNT         2626 a         1052 c         1290 g         2066 t
ORIGIN      
        1 aatttaaaat atattacctt attagaaaaa gtcgtaatat gaggtgtaca aatgacgcaa
       61 attttaatag tagaagatga acaaaactta gcaagatttc ttgaattgga actcacacat
      121 gaaaattaca atgtggacac agagtatgat ggacaagacg gtttagataa agcgcttagc
      181 cattactatg atttaatcat attagattta atgttgccgt caattaatgg cttagaaatt
      241 tgtcgcaaaa ttagacaaca acaatctaca cctatcatta taattacagc gaaaagtgat
      301 acgtatgaca aagttgctgg gcttgattac ggtgcagacg attatatagt taagccattt
      361 gatattgaag aacttttagc aagaattcgt gcgattttac gtcgtcagcc acaaaaagat
      421 attatcgatg tcaacggtat tacaattgat aagaatgctt ttaaagtgac ggtaaatggc
      481 gcagaaattg aattaacaaa aacggagtat gatttactat atcttctagc tgaaaataaa
      541 aaccatgtta tgcaacggga acaaatttta aatcatgtat ggggttataa tagtgaagta
      601 gaaacaaatg tcgtagatgt ttatataaga tatttacgaa acaaattaaa accatacgat
      661 cgtgacaaaa tgattgaaac agttcgtggc gttgggtatg tgatacgatg acaaaacgta
      721 aattgcgcaa taactggatt attgttacca cgatgattac gtttgtcacg atatttttgt
      781 tttgtttaat tattatcttt ttcttgaaag atacactgca taatagtgag cttgatgatg
      841 cagaaagaag ctcaagcgat attaataatt tatttcattc taagcctgtt aaagatatat
      901 ctgcattaga cttgaatgca tatttaggta attttcaaga gattattatt tatgatgagc
      961 ataataataa attatttgag acatcgaatg ataacacagt gagagttgaa cctggttatg
     1021 aacaccggta ttttgaccgc gtaataaaaa aacgttataa aggcattgat tatttaatta
     1081 ttaaagaacc aatcacaacg caagatttca aagggtatag tttgttaatt cattcactag
     1141 aaaattatga taacatcgta aaatcattgt atattattgc gctcgcattt ggagtgattg
     1201 caacaattat aactgccaca attagttatg tattttcaac tcaaattact aaaccgcttg
     1261 tcagtttatc aaataaaatg attgagatcc gacgagatgg ttttcaaaat aaattgcaat
     1321 taaatacaaa ttatgaagaa atagataatt tagcaaatac gtttaatgag atgatgagcc
     1381 aaattgaaga atcatttaat caacaaagac aatttgttga agatgcatca catgaattac
     1441 gaacaccatt acaaattatt caaggtcatt taaatttgat tcagcgatgg ggaaaaaaag
     1501 acccagcagt attagaagaa tcgttaaata tttctattga agaaatgaat cgtatcataa
     1561 aattagtcga agaattactt gaattgacta aaggagatgt aaatgacatt tcttctgaag
     1621 cgcagaccgt gcatattaat gatgaaattc gctcgcgaat acactcatta aaacaattgc
     1681 atcctgatta tcaatttgat acggatctga catctaaaaa tctagaaatt aaaatgaaac
     1741 ctcatcaatt tgaacaatta tttttaatct ttattgataa tgcaattaaa tatgatgtga
     1801 agaataagaa aattaaagtt aagacaaggt taaaaaataa gcaaaaaata attgaaatta
     1861 cagatcatgg aattggtatt ccagaggaag atcaagattt catttttgat cgcttttatc
     1921 gagtggataa atctcgttca agaagtcaag gtggtaatgg actcggatta tctattgcgc
     1981 aaaaaatcat tcaattaaac ggtggatcga ttaaaattaa aagtgagatt aacaaaggaa
     2041 caacgtttaa aatcatattt taatcatgtt taagacgtca atcaaagtca tagaatcaat
     2101 tttttaagtg aggctgggac ataaatcaat gttctatgct ttacgaagtt atattggctg
     2161 tagttgactg aacgaaaatg cgcttgtaac aagctttttt caattctagt caggggcccc
     2221 aacaaagaga aattggattc ccaatttcta cagacaatgc aagttggggt gggacgacga
     2281 aataaatttt gcgaaaatat catttctgtc ccactcccaa gaacgactat aaaacaaata
     2341 atcaggggtt ctttatcatt tctgtggaat tcacctccca aaatttacaa taaaatacat
     2401 ctataaacct agaagaatca acgcttttgt tgattcttct ttttagtaga taaataggta
     2461 aatctacttt aacaaataac taaatagtga tattattaca ttgtaagcgt ttcaacattt
     2521 ttgtggaggg tgtaaaatga ctaacgaaag aaaagaagtt tcagaggctc ctgtaaactt
     2581 cggtgcgaat ttaggtctaa tgttagatct atatgatgac tttttacaag atccatcatc
     2641 tgtaccagaa gatttacaag tcttattcag cacaattaag aatgatgact caattgtacc
     2701 agctttaaaa agtacaagta gtcaaaatag cgacggcaca attaagcgtg tcatgcgttt
     2761 gattgataat attcgccaat acgggcatct taaagccgat atttatcctg taaatcctcc
     2821 gaaaaggaaa catgtaccta aattagagat tgaagacttt gatttagatc aacagacttt
     2881 ggaaggtata tcagcaggaa ttgtttcaga tcactttgcc gacatttatg ataatgctta
     2941 tgaagcaatt ttaagaatgg aaaaacgtta caaaggacca attgcatttg agtatacaca
     3001 tattaataac aataccgaac gtggttggtt aaaaagaaga attgaaacgc catataaagt
     3061 aacgttaaat aataacgaaa aaagggcact atttaaacaa ttagcctatg ttgaagggtt
     3121 tgaaaaatat cttcataaaa acttcgttgg tgcaaagcgt ttttcaattg aaggggtaga
     3181 cgcacttgta ccgatgttac aacgtactat tacgattgct gcgaaagaag gtattaaaaa
     3241 tatacaaata ggcatggctc accgtggacg tttaaacgtt ttaacgcatg tcttagaaaa
     3301 accgtacgaa atgatgattt cagaatttat gcatacagat ccaatgaaat tcttacctga
     3361 agatggtagc ttgcagttaa ctgctggatg gactggtgat gtgaaatatc accttggtgg
     3421 cattaaaact actgattcat acggtacaat gcagcgtatt gcactggcta acaatccaag
     3481 tcacttggaa attgttgcac ctgttgttga agggcgtacg agagcagcac aagatgatac
     3541 gcagcgtgct ggggctccga cgactgatca tcataaagca atgccaatta ttatacacgg
     3601 cgatgctgct tatcctggtc aaggtattaa cttcgaaaca atgaacttag gaaacttgaa
     3661 aggctattct acgggtggtt cattgcatat tattactaac aatagaattg gatttaccac
     3721 agaaccaatt gatgcacgtt caacaactta ttctacagat gtggctaaag gttatgatgt
     3781 gccaatattc catgtcaatg cagatgacgt tgaagctact attgaagcaa ttgatattgc
     3841 aatggaattt agaaaagagt ttcataaaga cgtcgttatt gatttagtag gttatcgtcg
     3901 tttcggacat aacgaaatgg atgaaccatc aattactaat ccagttcctt atcagaatat
     3961 tcgcaaacat gactctgttg aatatgtgtt tggtaaaaag cttgttaatg aaggtatcat
     4021 ttcagaagat gaaatgcatt catttataga acaagtccaa aaggaactaa gacaagcaca
     4081 tgataaaatt aataaagctg ataaaatgga taatccagat atggaaaaac ctgcagaact
     4141 tgcattgccg ttacaagcag acgaacaatc atttacgttt gatcacttga aagaaataaa
     4201 tgatgcattg ttaacatatc cggatggctt taacattttg aaaaagttaa acaaagttct
     4261 tgagaagcgt catgagccgt ttaataaaga agatggttta gttgattggg cacaagcaga
     4321 acaacttgca tttgcgacaa tcttacaaga tggtacaccg attcgcttaa ctggtcaaga
     4381 tagtgaacgt ggtacattca gtcataggca tgccgtgtta catgatgagc aaacaggtga
     4441 aacatataca cctttacatc atgttcctga tcaaaaagcg acatttgata tacacaattc
     4501 tccgctttca gaagcagcag tagttggttt tgaatacggc tataatgtgg aaaacaaaaa
     4561 aagcttcaat atttgggaag cgcaatatgg tgattttgca aatatgtcac aaatgatttt
     4621 tgacaacttc ttattcagtt ctcgctcaaa atggggagaa cgttcaggat taacattatt
     4681 cttacctcat gcatatgagg gtcaagggcc tgaacattca tcagcaagat tagagagatt
     4741 tttacaatta gctgctgaaa ataattgcac agttgtcaac ttatctagtt caagtaatta
     4801 tttccactta ttgcgtgcac aagcagctag tttagattct gaacaaatgc gaccattggt
     4861 tgttatgtca ccaaaaagct tactgagaaa taaaacagtt gcaaaaccaa ttgatgaatt
     4921 tacttctggt ggatttgagc caattttgac agaatcatat caagcggata aagttacaaa
     4981 agttattttg gcaactggca aaatgttcat tgatttaaaa gaagcattag ctaaaaatcc
     5041 agacgaatca gtattactcg ttgcgattga aagattgtat ccattcccag aggaagagat
     5101 tgaagcattg ctagcacaat taccaaacct tgaagaagtg tcatgggtac aagaagaacc
     5161 taaaaatcaa ggtgcatggt tatatgttta tccatatgtt aaagtactag ttgcagataa
     5221 atatgattta agttatcatg gcagaattca aagggctgct ccagctgaag gcgatggaga
     5281 aattcataaa cttgttcaaa ataaaattat agaaaatgca ttaaaaaata actaggggga
     5341 aataagtcat gccagaggtt aaagttccag aattagcaga atctattaca gaaggtacca
     5401 ttgcagaatg gttgaaaaac ctaggggata gcgtagaaaa aggtgaagct attcttgaat
     5461 tagaaactga taaagttaat gtcgaagttg tatctgaaga agcaggtgta ttatctgaac
     5521 aacttgcaag tgaaggcgac actgtagaag ttggacaagc aattgctgtc atcggcgaag
     5581 gtagtggcaa tgcttctaaa gaaaatagta acgacaatac tccacaacaa aatgatgaaa
     5641 caacaaataa taaaaaagaa gaaacaacaa ataaatcggc agataaagct gaagtcaatc
     5701 aaacaaatga tgacaatcag cagcgtgtta atgctacacc ttctgcgcgt cgatatgctc
     5761 gtgaaaatgg tgtgaatctt gctgaagtaa gtccgaaaac aaatgatgtt gttcgtaaag
     5821 aagatattga taagaaacaa caggcacctg catcaacaca aacaacacaa caagcacctg
     5881 caaaagaaga gaaaaaatac aatcaatatc ctacaaaacc agtgattcgt gaaaaaatgt
     5941 cacgcagaaa gaaaacggct gctaaaaaat tattagaggt atctaataat acagctatgc
     6001 taacaacatt taacgaagtt gacatgacaa atgttatgga attgcgtaaa cgtaagaaag
     6061 aacaatttat gaaagatcat gatggtacta aattaggatt tatgtcattc tttactaaag
     6121 cttctgtagc agctttgaaa aagtatccag aagttaatgc agaaatcgac ggcgacgaca
     6181 tgattacgaa acaatattat gatattggtg tagctgtttc gacagatgat ggactattag
     6241 taccattcgt aagagattgt gataaaaaga attttgcaga aatcgaagca gaaattgcta
     6301 atttagctgt taaagcacga gagaaaaaac ttggcttaga tgatatggtt aatggttcat
     6361 ttacgattac aaatggcggt atttttggat caatgatgag tacgccaatt atcaatggta
     6421 atcaagctgc aatcttaggc atgcattcaa ttattacaag accaattgcg attgatcaag
     6481 atacaatcga aaatcgtcca atgatgtata ttgcattaag ctatgatcat agaattattg
     6541 acggtaaaga agcagttgga ttcttaaaaa caattaaaga attaattgaa aacccagaag
     6601 acttattatt agaatcttaa tccacaacac aaaatagttt actattccta aaaacgggat
     6661 caaatcaata acaaacagca gtaagtttat tttctagtcg aaatatctta ctgctgtact
     6721 ttatttttac aatgcttcgt attaaaatgt gagtttaatt ttgactactt aaaaaatata
     6781 tcatttctat atgaaataga ctcacgatag aaatatagct agaatagcgt gtttcaatat
     6841 gctaatgaaa tcattttaga taataacaag cgatgaaatt tatgaattct ataagcgcct
     6901 ttagttaatt taaaatcatc tgaacaagat gtacattcac tttatggaca cgacatatat
     6961 atgtttttca tgtactacag aaaaacgaaa tatcccaata tccaatcgct atatacagat
     7021 actcattaat gaca
//
LOCUS       DAKSQE010000119         6818 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.119, whole genome shotgun sequence.
ACCESSION   DAKSQE010000119 DAKSQE010000000
VERSION     DAKSQE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.119"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(117..677)
                     /gene="icaR"
                     /locus_tag="O0V33_002260"
     CDS             complement(117..677)
                     /gene="icaR"
                     /locus_tag="O0V33_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HCX8976875.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNT
                     PEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQ
                     KFGAVEESKSQFKDEVYSLLNIFLKK"
     gene            841..2079
                     /gene="pgaC"
                     /locus_tag="O0V33_002261"
     CDS             841..2079
                     /gene="pgaC"
                     /locus_tag="O0V33_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HCX8976876.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLCYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            2043..2348
                     /gene="icaD"
                     /locus_tag="O0V33_002262"
     CDS             2043..2348
                     /gene="icaD"
                     /locus_tag="O0V33_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HCX8976877.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            2345..3217
                     /gene="icaB"
                     /locus_tag="O0V33_002263"
     CDS             2345..3217
                     /gene="icaB"
                     /locus_tag="O0V33_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HCX8976878.1"
                     /translation="MKYRKLIILVLSILIILPVSTLDGHHIANADDDPPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKWLKSHDAKFLTLKEF
                     LYYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPATGFIITGHVGEENFHNLD
                     MISKKELKEMYKTGLWEFETHTHDLHNLTKNNKSKLMKASEATIIKDLNKSEKYLTKN
                     FKKSQKTIAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVTPNSNDYYIPRILISDDAF
                     EHLIKRWDGFHEKD"
     gene            3204..4256
                     /gene="icaC"
                     /locus_tag="O0V33_002264"
     CDS             3204..4256
                     /gene="icaC"
                     /locus_tag="O0V33_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HCX8976879.1"
                     /translation="MKKIRLELVYLRAIICAIIIVTHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMSLFYSYSESLL
                     TDTSFQKQFIENVLLGQWYGYFIVVIMQFFILSYIIFKINYNLFNSKILLLLSFILQQ
                     SFLYYFTNNTTFHDTVLHYYPLSENTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVI
                     MIVLAVATYFVFIALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQM
                     ISAFSFFIYLLHPIILDSLFAYTNIFEDNTMVFLAISLLFILGLCIGVGMILREFYIF
                     RFIIGKQPYKLNINAY"
     gene            complement(4591..6633)
                     /gene="lip1"
                     /locus_tag="O0V33_002265"
     CDS             complement(4591..6633)
                     /gene="lip1"
                     /locus_tag="O0V33_002265"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HCX8976880.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLN
                     KDQLKDGKKSSLNNKNIQRDTTKNNNANPSDVNQGLEQAINDGKQSKVASQQQSKEAD
                     NSQDSNANNNLPSQSRTKEAPPLNKSDQTSQREIVNETEIEKVQPQQNNQANDKITNY
                     NFNNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKDNDKKNGLNLLKSSAVATLPNKGT
                     KELTAKAKGDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELMRNGSREEIEYQKKHGGEISPLFKGNNDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTNATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKVTDTKQA"
BASE COUNT         2154 a         1100 c         1019 g         2545 t
ORIGIN      
        1 tacatcttaa tattctcaat ttcgagttgt attgtcaact ttatatagat ttaaaaaaat
       61 aatctcatgt ctttttttac aaaagtaagt taattattac aaactagtaa caaaaattat
      121 ttcttcaaaa atatatttag tagcgaatac acttcatctt tgaattgact tttactttct
      181 tccactgctc caaatttttg cgaaaaggat gctttcaaat accaactttc aagaaacagc
      241 aatattaaat tctgaaagtc ttcttttgtc atctttatct ttgattcatc atagaatttt
      301 gctatctctt tacttaacga ttgatttaaa tcttgtattt gtccgtaaat atttccagaa
      361 aattcctcag gcgtattaga taattgaacg tacattctaa tatacctttc ttcgatgtcg
      421 aaaataaact caaataagaa ttgatataaa gcatcaattg aatagttcga tttattttga
      481 ttcatcataa taatattatt aaggtaatca aaacagcatt taacactttg ttcgtaaata
      541 ctttttttcg agtcaaaatg gtaatataaa ctcgcttttt ttatatttac acttttagct
      601 atatcatcaa gtgttgtacc gtcatacccc ttctctgaaa ataaggttat tgcgttatca
      661 ataatcttat ccttcaattt ttaaaacccc ctactgaaaa ttaatcacac tatgttacag
      721 gaaaattaag ttgcaattac aaatatttcc gtttaattat aacaacaatc tattgcaaat
      781 taaaatacta tcaattacca tatggcttac aacctaacta acgaaaggta ggtaaaggaa
      841 ttgcaatttt ttaacttttt gcttttttat cctgtattta tgtctattta ctggattgtc
      901 ggttcaattt atttctattt tactagagaa attagatatt cattgaacaa gaagcctgac
      961 ataaatgtgg atgaattaga aggcattaca tttttacttg cctgttataa cgaaagtgaa
     1021 acgattgaag atacgttgtc taatgttctt gcactcaaat acgagaaaaa agaaattatt
     1081 atcattaatg atggaagttc agataataca gcagaactca tctataaaat taaagaaaat
     1141 aatgacttta tttttgtcga tttacaagaa aacagaggta aagccaacgc actcaatcaa
     1201 ggcattaaac aggcttcata tgattatgta atgtgcttgg atgcagatac tatcgttgat
     1261 caagatgcac catattatat gattgaaaat ttcaaacatg atccaaaact tggtgcagtt
     1321 acaggtaatc ctagaattcg aaataagagt tctattttag gtaaaattca aacgatagaa
     1381 tatgcaagtt taattggctg tattaagcga agtcagacac ttgctggcgc agtcaatact
     1441 atttcgggtg tcttcactct atttaaaaaa agtgcagttg tcgacgttgg ctactgggat
     1501 actgatatga ttaccgaaga tattgcagtt tcttggaaat tgcatttacg tggatatcgt
     1561 attaagtatg aaccgcttgc catgtgttgg atgttggttc cagaaacatt gggaggtctt
     1621 tggaagcaac gcgtgagatg ggctcaaggg ggacacgaag tattactacg agactttttt
     1681 agcacaatga aaacgaaaag gtttccttta tatattttga tgtttgagca aatcatctca
     1741 attttatggg tatatatagt gcttctatat ttatgctatt tgttcataac agcaaacttc
     1801 ttagactata catttatgac atatagtttt tcaatatttc tactatcatc atttactatg
     1861 acttttataa acgttattca atttacagtc gcactcttta ttgatagtcg ctacgagaaa
     1921 aagaatatgg ctggactcat atttgtaagt tggtatccga cagtatactg gattattaac
     1981 gcagcagtag ttcttgtcgc atttccaaaa gcattaaaac gtaagaaagg tggttacgca
     2041 acatggtcaa gcccagacag agggaatacc caacgctaaa atcatcgcta aatattgtaa
     2101 gagaaacagc acttatcgct atatcgtgtg tcttttggat atattgttta gttgttctac
     2161 tcgtttatat tggtactata tttgaaattc atgacgaaag tatcaataca atacgtgttg
     2221 ctttaaacat tgaaaatact gaaattttag atatatttga aactatgggc attttcgcga
     2281 ttatcatttt tgtatttttt acaattagca tattgattca aaaatggcag agaggaagag
     2341 aatcgtgaag tatagaaaat taataatttt agtgttgagt atcttgatca tattgcctgt
     2401 aagcacactg gatggtcatc atattgcaaa tgcagatgac gatccaccta aaaaactgaa
     2461 atataaagaa aatagtgctc tggcattaaa ttatcaccgt gtaagaaaag cgaattttct
     2521 gaataatttc atttacttct tttctagtag taaagaaatt aaaaattata gtgttagtca
     2581 atcacaattt gaatctcaaa taaaatggct aaaatcacat gatgctaaat ttttaacctt
     2641 gaaagaattt ttatattaca agaaaaaagg taagtttcca aaacgaagtg tatggattaa
     2701 ctttgatgat atggatgaaa ctatttatga aaatgcttat ccaatcttaa aaaaatataa
     2761 aataccggca actgggttta ttatcacagg tcatgttggg gaagaaaact ttcacaacct
     2821 cgatatgatt agtaaaaaag aactaaaaga aatgtataaa actgggttat gggaatttga
     2881 aacacatacc cacgatttgc ataacttaac taaaaataat aagtcaaaat taatgaaagc
     2941 ttctgaagct acaatcataa aagatttaaa caaaagtgaa aaatatctaa ctaaaaactt
     3001 taaaaagtcg cagaaaacta tagcctatcc ttatggcttg atgaatgacg ataaattacc
     3061 ggtaattaaa aaagctgggt taaaatacgg tttttcatta gaggaaaaag cagtcactcc
     3121 gaactccaat gattattaca tccctagaat attaattagt gatgatgctt ttgagcattt
     3181 aattaagaga tgggacggat tccatgaaaa agattagact tgagctcgta tatttacgtg
     3241 ccattatatg tgcaattatt attgttacac atttattaac acaaattact ttaaaacatg
     3301 agaatatgga aggaggatct ttagtactac agttctatat tcgtaatatt gtcatttttg
     3361 gtacaccttg ctttattatc ttgtcacagt tactgacaac cttgaattac caaaaagtca
     3421 cctatagata cttaactaca cgcgtaaaat atatacttat tccttacata ttaatgagtt
     3481 tattttacag ttatagtgaa tcattattaa ctgacacaag ttttcaaaaa caatttattg
     3541 aaaatgtcct attaggtcaa tggtatggct attttatcgt tgttatcatg caattcttta
     3601 ttttgagtta tatcattttt aaaattaact ataacctatt caacagtaaa atattattat
     3661 tgttatcttt tattttacag caatcatttt tatattactt tacgaacaac acaacgtttc
     3721 acgataccgt gctacactat tatccattaa gtgaaaacac tataatattc ggatggattt
     3781 tttatttctt tttaggtgca tatatgggtt ataactacga acgtgtatta aatttcttag
     3841 aacgttattt agttattatg attgtattag ctgtagctac ttattttgtg tttattgcgt
     3901 tagcaaatgg agactattgg aacgttacca gcttttcata ttcattaaca ccatataata
     3961 gtattatgtt tattgttatc ttgggtattt gcacgcattt taaaacaatg ttatttaata
     4021 cgattcaaat gattagtgct ttctcattct ttatttattt attacatcca atcattctag
     4081 actcattgtt tgcatataca aatatatttg aggataatac aatggtcttt ctagcgatat
     4141 cactactatt cattttagga ttatgtatag gtgtcggtat gatattgcgt gaattctata
     4201 tctttaggtt tattattgga aaacaaccat ataaattgaa cattaatgct tattaattat
     4261 taagctatgt taaaaacacg cggtgggcga aatcagtttg aattgactga cttcgtttta
     4321 ccgcgtgttt aatattgtta tacatatatt cttattgcac atttaaacta cgtaaatgcc
     4381 aatgggagtg gtacagaaat gatattttcg caaaatttat ttcgtcgtcc caccccaact
     4441 tacattgtct gtagaatttc ttttcgaaat tctctgtgtt ggggccccgc caaggtatta
     4501 cttgaataac tcttttagaa ttctaaataa tgatccgatt aattgaaaga agtctgcagt
     4561 cattattaat tcctcccttt actttataaa ttatgcttgc ttagtatcag tcaccttttc
     4621 agttttcact aaatcatctg ctaaatgatg ccaaaaatct tgtaattctt ctcttgtgcg
     4681 cactgtatca gaactatctt ggccgacaaa atcaacatga tcccaatcat gttttgtagg
     4741 cgttacttgc caaatgcctt tttgaatttt atccgtcgca tttgtataag cttgattaaa
     4801 tggatgttga gaagaaataa cggatactaa accatcgttt tctcgccatt ctttttcagt
     4861 agctttaccg attaagttac cagtaatcac aaatgggaaa aacatattta agtctgcttt
     4921 ttgtctatcg ctatttaatg ctttgtgcgt tgcttcacca gtgtatgttt tatacacaat
     4981 gttagggttc aacgacgttt tacgatttaa atctgttgca ccctcacgcg tcagatcgta
     5041 aaatccatta tcttttgatt tccataaatt agattgttta acacgtttga cataatcaat
     5101 atatgattca tttggcttct gttttagacc ccattgagcc aacccgaagt ctactcttga
     5161 atttttatta ccaaacattt taccgatatc aaatacgatt tgtctcacta aagcttcatt
     5221 accagctaaa tctgatgcgt gcgttccatt atgtggcgtt cctaaagtag taattgatga
     5281 aatcatattg tcattattac ctttgaatag tggagaaatt tcgccaccat gtttcttttg
     5341 atactctatt tcttcacgac taccattacg cattaattct tctagttgac gtatcgtttg
     5401 accacccata ctatgtccaa caaggtgtac cttctgtcct ggtttccagt ctttgtaaat
     5461 tccttcgtat gtttttccat aacgttcatg tccatatttt gctgcatgtg ctgcaccata
     5521 atctacacga ccgcctttga tataataata aagttcaact gcgcggtcat agttacttcc
     5581 aaaagcactt atacttgctt cataagcttt gtaaccattt tcttctaaat cttggcgaat
     5641 gttcatttta ttaccgcccc aataatgagc taacactgaa ggattaatat catctgtaaa
     5701 cccattgaaa ccatgcacta aaacgatagg atcttgattt ttatactgcc cttgtttggc
     5761 aactttattc gtttgatcac ctttcgcttt tgcagtaagt tcctttgtcc ctttgtttgg
     5821 taacgttgct actgcactac tttttaataa atttaatcca tttttcttgt cattgtcctt
     5881 tgttggttct actggtgatt tttgattgtt ttttaaatct gaaactgata gtgttttttc
     5941 gtctttttga ggtttcactt cttgttcatt gttaaaattg tagttagtaa ttttatcatt
     6001 cgcttgatta ttttgttgcg gttgtacttt ctctatttct gtctcattaa caatttctcg
     6061 ttgacttgtt tgatctgact tatttaatgg tggtgcttcc tttgttcgac tttgtgaagg
     6121 tagattgtta ttagcgtttg aatcttgact attatctgcc tcttttgact gttgctgtga
     6181 cgccacttta ctttgtttgc catcattaat agcctgttct aacccttgat ttacatcgct
     6241 aggattagca ttgttatttt ttgttgtatc acgttgaata tttttattat taagcgatga
     6301 ttttttacca tcctttagtt gatctttatt caaatcatct gttttacgat gattttcttc
     6361 tgatattgtt ttatcattat gcaagttctt agttaaattt tcagtatgtt gctgttcgtc
     6421 aacaccgttt tcacgttgat aattagcatt ttccctagtt tgttgatccc caatagattg
     6481 tgctgtaact gtatcctcct gtgaatttcc catattcact tgcttctcag ctgcttgtgc
     6541 ttgtccacca cttaaaaata gtaatgtagc tattaaaatg gaagatgcac ctacactaaa
     6601 tttacgaata ctatacttat tttgactttt catcattgtc agcacctcta tttttttatt
     6661 ttagataaac aaattgataa ttattcgagg aataaactca aataatttac ttaaccaagc
     6721 actcataatg accatccttt ctattgttta atttgacgag aaagcgttta catttttatt
     6781 taaaaaatat attctccaaa gagaatttaa agtgtata
//
LOCUS       DAKSQE010000120         6703 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.120, whole genome shotgun sequence.
ACCESSION   DAKSQE010000120 DAKSQE010000000
VERSION     DAKSQE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.120"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            143..298
                     /locus_tag="O0V33_002266"
     CDS             143..298
                     /locus_tag="O0V33_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976881.1"
                     /translation="MIVKKLLKAIKQNFLYEQSNITFKMILFGYKRLSKMIFQFEINH
                     MNFLREG"
     gene            306..827
                     /locus_tag="O0V33_002267"
     CDS             306..827
                     /locus_tag="O0V33_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976882.1"
                     /translation="MINIISAIGSIGTFIMALFYFVSVSVQLYQMKISFLPALGFNQI
                     LLEREGDQLNIMNSATEEHHHKDYIKLYNLGGGAAKKIAIEVLLGNDKVIQKKYVNIL
                     PSKEGYMLSINKNVYEELERTIENNGYEADLNVRMTYYHNVSHKQQEVILKGQIDRFN
                     TYNNKEIYDLQFI"
     gene            966..1496
                     /locus_tag="O0V33_002268"
     CDS             966..1496
                     /locus_tag="O0V33_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCX8976883.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNNLELVAVLQNEVVGHGLLSEVYLDNEAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPNYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
     gene            1760..4831
                     /gene="clfA"
                     /locus_tag="O0V33_002269"
     CDS             1760..4831
                     /gene="clfA"
                     /locus_tag="O0V33_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HCX8976884.1"
                     /translation="MNMKKQEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSM
                     TQTENTSNESKSNDPSSVNAAPKTDNTNVSDSNTTTNTNSDETNVAQNPAQQETTQSA
                     STNATTEETPVTGEVTTTATNQANTPATTQSSNTNAEESVNQTSNETTSNDTNTVSSV
                     NSPQNSTNAENVSTTQDISTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSL
                     AAVAADAPAAGKDITNQLTNVTVGIDSGDTVYPHQAGYVKLNYGFSVPNEAVQGDTFK
                     ITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLT
                     MPAYIDPENVTKTGNVTLATGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNN
                     TYRQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDAQNTSIKVYKVDNASDLSESYYV
                     NPDNFEDVTDSVNITFPNPNQYKVEFNTPDDQITTPYIVVVNGHIDPNSKGDLALRST
                     LYGYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPG
                     SDSGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASD
                     SDSASDSDSTSDSDSTSDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSVSDSDSDSDSDSESDSD
                     SDSDSDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSDSDSDLDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDLDSDSDSDSDSDSDSDSASDSDSDSDSDSDSESDSDSDSDSDSDSD
                     SASDSDSDSDSASDSDSDSTSDTGSDNDSDSESDSNSDSDSGSNNNVVPPNSPKSGTN
                     ASNKNEAKESKEPLPDTGSEDEANTSLIWGLLASLGSLLLFRRKKENKDKK"
     gene            5052..6559
                     /gene="vwb"
                     /locus_tag="O0V33_002270"
                     /pseudo
     CDS             5052..6559
                     /gene="vwb"
                     /locus_tag="O0V33_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001628779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor binding protein Vwb"
BASE COUNT         2574 a         1021 c         1260 g         1848 t
ORIGIN      
        1 attcaactta tttgtatgag atgaatatgt attgaagaag atgttttatt aattttgaaa
       61 tactggtcaa agatgggtgc cctcaaaagc gttattgtat tttttagtca atgcaaatag
      121 attgccgtaa taataagcgt acttgatagt taaaaaatta cttaaggcta taaagcaaaa
      181 ttttttatat gagcagtcga atataacgtt taaaatgatt ctttttggat ataaacgatt
      241 aagtaaaatg atttttcagt ttgaaattaa tcatatgaat ttcttaagag aagggtgata
      301 tcttaatgat taatattatt tcagctatag gatctattgg aacatttatt atggctttat
      361 tttattttgt atcagtttca gttcaacttt atcaaatgaa aattagcttt ctgccagctt
      421 taggttttaa ccaaatttta ttagaaaggg agggggatca acttaatata atgaattccg
      481 ctacagaaga gcatcatcat aaagattata ttaaactata taatttaggt ggcggtgctg
      541 ctaaaaaaat tgcaatagag gttttattgg gtaatgataa agtcattcag aaaaagtacg
      601 tgaatatttt acctagtaaa gaagggtaca tgttatcaat taataaaaat gtgtacgaag
      661 aattagaaag aacgattgag aacaatggtt atgaagctga tttgaatgta cgtatgactt
      721 attatcataa tgtaagtcac aaacaacagg aagttatatt aaaaggtcaa atcgaccgtt
      781 ttaatactta taataataaa gaaatttatg atttgcagtt tatctgaaaa ttgatttaag
      841 aggatagttg tttattgcaa aaaatatcat tcaattttaa tgaaataatg gcgtcattac
      901 tataaaatat tactttatgt tgtaatgcat ttttctataa gatagaacta aaaggagggg
      961 caaagatgca aattagacaa atacatcaac atgactttgc tcaagttgac cagttaatta
     1021 gaacggcatt tgaaaatagt gaacatggtt atggtaatga atcagaacta gtagaccaaa
     1081 ttcgtctaag tgatacgtat gacaataact tagaattagt agctgttctt caaaatgaag
     1141 ttgtagggca cggtttacta agtgaagttt atcttgataa cgaggcacaa cgggaaattg
     1201 gattagtgtt agcacctgta tctgttgata ttcatcatca aaataaaggt attgggaagc
     1261 gattgattca agcattagaa cgagaagcaa tattaaaagg atataatttt atcagtgtat
     1321 taggatggcc gaattattat gccaatctag gatatcaacg cgcaagtatg tacgacattt
     1381 atccaccata tgatggtata ccagatgaag cgtttttaat taaagaatta aaagtgaaca
     1441 gtttagcggg aaaaacaggt accataaatt acacatctgc ttttgaaaaa atatgatttc
     1501 aagctaggat tacattaggc agagttcata ttaataatac aaattgttta caatgaaatc
     1561 gcgcattgtc gtttgcaatt tttaaaacag agaaaatgaa aaacttttcg ttgtaaatat
     1621 ttattgtagt taataaaatt ctaaaacaaa ttaattgcca ttatgcaatt ttggtgtata
     1681 attgcattaa tagagattaa atatatatta aaagggtata cagttaatat aaaatgactt
     1741 tttaaaaaga gggaataaaa tgaatatgaa gaaacaagaa aaacacgcaa ttcgtaaaaa
     1801 atcgattggc gtggcttcag tgcttgtagg tacgttaatc ggttttggac tactcagcag
     1861 taaagaagca gatgcaagtg aaaatagtat gacacaaacg gaaaatacga gtaatgagag
     1921 caaaagtaat gatccaagta gcgttaatgc tgcacctaaa acagacaaca caaacgtgag
     1981 tgattctaat acaacgacaa acactaatag tgacgaaacg aatgtagcgc aaaatccagc
     2041 acaacaggaa acgacacaat cagcatcaac aaatgcaact acagaagaaa caccggtaac
     2101 tggtgaagtt actactacgg caacgaatca agctaataca ccggcaacaa ctcaatcaag
     2161 caatacaaat gcggaagaat cagtgaatca aacaagtaat gaaacgactt ctaatgatac
     2221 taatacagta tcatctgtaa attcacctca aaattctaca aatgcggaaa atgtttcaac
     2281 aacgcaagac atttcaactg aagcaacacc ttcaaacaat gaatcagctc cacagagtac
     2341 agatgcaagt aataaagatg tagttaatca agcggttaat acaagtgcgc ctagaatgag
     2401 agcatttagt ttagcggctg tagctgcaga tgcaccggct gctggcaaag atattacgaa
     2461 tcagttgacg aatgtgacag ttggtattga ctctggagat acagtttatc cgcaccaagc
     2521 aggctatgtc aaactgaatt atgggttctc agtaccaaat gaggctgttc aaggtgacac
     2581 attcaaaata actgtgccca aagaattaaa cttaaatggt gtaacttcaa ctgctaaagt
     2641 gccaccaatt atggccggag atcaagtatt ggcaaatggt gtaatcgata gtgatggtaa
     2701 tgttatttat acatttacag actatgtaaa tactaaagat gatgttaaag caactttgac
     2761 catgcccgct tatattgacc ctgaaaatgt tacaaagaca ggtaatgtga cattggctac
     2821 tggcataggt agtacaacag caaacaaaac agtattagta gattatgaaa aatatggtaa
     2881 gttttataac ttatctatta aaggtacaat tgaccaaatc gataaaacaa ataatacgta
     2941 tcgtcagaca atttatgtca atccaagtgg agataacgtt attgcgccgg ttttaacagg
     3001 taatttaaaa ccaaatacgg atagtaatgc attaatagat gcacaaaata ctagtattaa
     3061 agtatataaa gttgataatg catcagactt gtctgaaagt tattatgtga atccagataa
     3121 ctttgaagat gtcactgata gtgtgaatat tacattccca aatccaaatc aatataaagt
     3181 agagttcaat acgcctgatg atcaaataac aacaccatat attgtagttg ttaatgggca
     3241 tattgatcct aatagtaaag gtgatttagc tttacgttca actttatatg gatataattc
     3301 gaatataatt tggcgatcaa tgtcatggga taatgaagta gcatttaata acggatcagg
     3361 ttctggtgac ggtatcgata aacctgttgt tcctgaacaa cctgatgagc cgggtgaaat
     3421 tgaaccaatt ccagaggatt cagattctga cccaggttca gatagtggtt cagattctgg
     3481 cagcgattct aattcagata gcggttcaga ttcgggtagt gattctacat cagatagtgg
     3541 ttcagattca gcgagcgatt cagattcagc aagtgattca gattcagcga gtgattcaga
     3601 ttcagcaagt gattcagatt cagcgagtga ttcagattca gcaagtgatt cagattcaac
     3661 gagtgattca gattcaacga gtgattccga ttcagcaagc gattcagatt cagatagtga
     3721 ctcagactca gacagcgatt cagattcaga tagtgactca gactcagata gcgattcaga
     3781 ttccgacagt gactcggatt cagatagcga ttccgactca gacagtgact cagattcaga
     3841 tagcgactca gactcagaaa gcgactcaga ttcagacagc gattccgact cagatagtga
     3901 ttcagattca gacagcgact cagacagtgt ttcagattca gacagtgact cagactcgga
     3961 tagtgaatca gactcagaca gcgactcaga ttcagacagc gactcagact cggatagtga
     4021 atcagactca gacagtgact cagactcaga cagcgactca gactcagata gcgactcaga
     4081 ttcagatagc gattcagact cagacagcga ctcagactca gatagcgatt ccgactcgga
     4141 tagcgactca gactcagata gcgattccga ctcggatagc gattcagaat cagatagtga
     4201 ctcagattca gatagcgatt ccgactcaga tagcgactca gattccgaca gcgattcaga
     4261 ttcagacagt gatttagact cagacagtga ctcggattca gatagcgatt ccgactcaga
     4321 cagtgactcg gattccgaca gcgattcaga ttcagacagt gatttagact cagacagtga
     4381 ctcggattca gatagcgatt ccgactcaga tagtgcctca gattcagata gcgactcaga
     4441 ctcagacagc gattcagaat cagatagtga ctcagattca gatagcgatt ccgactcaga
     4501 cagtgcctca gattcagata gtgactcgga ttcagcgagt gattccgatt cagattcaac
     4561 gagtgacaca ggatcagata acgactcaga ctcagaaagt gattcgaata gcgattccga
     4621 ttcaggttct aacaataatg tagtgccgcc taattcacct aaaagtggta ctaatgcttc
     4681 aaataaaaat gaggctaaag aaagtaaaga accattacca gatacaggtt ctgaagatga
     4741 agctaatacg tcactaattt ggggattatt agcatcatta ggttcattac tacttttcag
     4801 aagaaaaaaa gaaaataaag ataagaaata agtaataatg atattaaatt aatcgtatga
     4861 ttcatgaaga agtcacctaa aaaggtggct tttttacttg gattttccaa atatattgtt
     4921 tgaatataat taataattaa ttcatcaaca gttaattatt ttaaaaaggt agatgttata
     4981 taatttggct tggcgaaaaa atagggtgta aggtaggttg ttaattaggg aaaattaagg
     5041 agaaaataca gttgaaaaat aaattgctag ttttatcatt gggagcatta tgtgtatcac
     5101 aaatttggga aagcaatcgt gcgagtgcag tggtttctgg ggaggagaat ccatataaat
     5161 ctgagtcatt gaaattaaat gggaaaagaa gtactacaat aactagtgat aaatatgaag
     5221 aaaatttaga tatgttaata tcgtcattat catttgcaga ttatgaaaaa tatgaggaac
     5281 cagaatacaa agaagcagtt aaaaagtatc aacaaaaatt tatggctgaa gatgatgcat
     5341 taaaaaattt tttagtgaag agaaaaaaat aaaaaataga aatactaata catcaaatta
     5401 tctgggatta acacacgaaa gatatgagtc aatttataat tcattaaaaa atcatcgtga
     5461 agaattttca aaagaaatcg aagaaattaa taataaaaat ccagtgttaa aagaatataa
     5521 caatgaggaa caaactaaag ctgatacgga attaaacact cttgaaaatc aagtactaat
     5581 gataggttat acattttatc actcgaataa aaatgaagta gaagatttat ataacaaatt
     5641 agatatgatt cttggttata aagatgaaga gagaaaaaag aagagggcta ccaatcaaag
     5701 aatgttcaat aataaaaaag aggatttaga aactattatt gatgaattct ttggagaaat
     5761 tggacaacaa aggccaacat ctataccaac attagcgcct aaagaagaaa aagaaacaaa
     5821 tataaaaaat gcaaataaat taaaatctga cactgaagca gcaaaaaatg atgaagcaaa
     5881 aagaagttta aatacccaca atcacaaatc tgtatctcaa gaagtctctg aacaacaaaa
     5941 agctgactac gaaagaaaag ctgaagaaag aaaagcgaga tttttagata agcaaaaaaa
     6001 taagaaaact cctgtagttt cattagaata tgattttgaa cataaacaac gtgttgacaa
     6061 cgaaaacgac aagcaacttg tggtttctga gccatcaaag aaaccaacaa caccgcctac
     6121 atacactgaa acaaccacac agctaccaat gcctacagtt gagcgtcaaa cacagcaaca
     6181 aatcgtttac aaagcaccaa aaccattagc tggattaaat ggtgaaagtc atgatttcac
     6241 aacaacgcat caatcaccaa ctacttcaaa tcacacgcat aatcatctta ttgaaattga
     6301 agaaacatct gctttacctg gtagaaagac aggttcattg gttggtttga gtcaaattga
     6361 ttcttcgcat ttaactgaac gtgagaagcg cgtgattaaa cgtgaacacg tgagagaagc
     6421 tcaaaagtta gttgataatt ataaagatac acatagttat aaagaccgat taaatgccca
     6481 acaaaaagta aatactttaa gtgcaggtca tcaaaaacgt tttaataaac aaattaataa
     6541 agtatataat ggcaaataat taatgcatgg ctgcaaagga aataatgagt ttgccgtaaa
     6601 aataacaaca ttttaaacta gcaataaata atatcaaagt catcatttca atgatgcaat
     6661 ctagtatagt ccacattcta aacaggtgtg gactattact ttt
//
LOCUS       DAKSQE010000121         6635 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.121, whole genome shotgun sequence.
ACCESSION   DAKSQE010000121 DAKSQE010000000
VERSION     DAKSQE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.121"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..496)
                     /locus_tag="O0V33_002271"
     CDS             complement(<1..496)
                     /locus_tag="O0V33_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCX8976885.1"
                     /translation="MIRNRLSELLSERGLKISRVAKDVKIARSSLTSMAQNDSEMIRY
                     DAIDKLCSYLHISPSEFFEHNPINFDFTFDEEPNYKINDVFEGFEVTANITHAFSIEN
                     FDFEILVDVELDNRQKLNFDLDVSYKETEKITNSQHRFIFTIKNEDENIGLKKYVDSL
                     SAGLK"
     gene            669..887
                     /locus_tag="O0V33_002272"
     CDS             669..887
                     /locus_tag="O0V33_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCX8976886.1"
                     /translation="MTILANTRKFKEAMFLKGFNLSDLSRETGVGISYLSQIINGKKI
                     PSPKLAKKMAEVLQVEVNELFEFEVKEA"
     gene            892..1209
                     /locus_tag="O0V33_002273"
     CDS             892..1209
                     /locus_tag="O0V33_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCX8976887.1"
                     /translation="MFNINIDEDEARELLEQAINARVDELAKEKYFMTYKELSNYLNL
                     SKPTIEELLINNGMKYYMVGSTYRFKKSDVDEFMEQLTAHMNIQNNDFKQVNIKKLLE
                     ARQ"
     gene            1206..1361
                     /locus_tag="O0V33_002274"
     CDS             1206..1361
                     /locus_tag="O0V33_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCX8976888.1"
                     /translation="MKIYLTYICLVSLLTILLLAISNMYVAFSVYAWLITLGCNLTGE
                     ITTCENK"
     gene            1346..1549
                     /locus_tag="O0V33_002275"
     CDS             1346..1549
                     /locus_tag="O0V33_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCX8976889.1"
                     /translation="MRKQVIITKTVVGWYNIKDTQHNLMLNIPPKVFEQYFPDVSKDV
                     QVVCLEMDLSKITEIKNKKKVGS"
     gene            1551..1934
                     /locus_tag="O0V33_002276"
     CDS             1551..1934
                     /locus_tag="O0V33_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCX8976890.1"
                     /translation="MEIKQKYQLSKVVKILEVVLYEEDKFQSDKDYHYQDKALYEYAL
                     KLVHNGLFNILAELDFEDEAFLILDEVTMTLSDVMKETQHVYRYSVIDEKGEHKHTTD
                     RKGHVIGMLEWALDYIAGNIEVEEL"
     gene            1935..2252
                     /locus_tag="O0V33_002277"
     CDS             1935..2252
                     /locus_tag="O0V33_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1474 family protein"
                     /protein_id="HCX8976891.1"
                     /translation="MNWEIKDLMCDIEAVKEKINDVAIKHAWFVEDRFVKNELETKRE
                     HINFSASYLEHRIQNEHTVELLHVYLKEFGELIQKFHEIEKASSENFGEVSDDAQKLK
                     ITE"
     gene            2316..3185
                     /locus_tag="O0V33_002278"
     CDS             2316..3185
                     /locus_tag="O0V33_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001667297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HCX8976892.1"
                     /translation="MNEIKLEYDTHVSVVHYESLDSRSFNSFSKINWSKLVNKLSVPI
                     EANYKYARGVAVYGDIKNGANDQGEIIKKHRNDKNVIYRDVIVLDYDEINDLKQLHEA
                     ISSALSNVAWFWHTSYSHRTEQARIRLYIPLNERISADDYRKYTKVLANKIGHKVDEG
                     SYQPSRCFALPVIQKGHIFIKRVNDCPIMNVDMLEQWSKEFEQSNASPNVIGYTRRDS
                     EYWRELCFGTTEGNRNNALASLIGHLLRCHVNDYIVYSFALLWGQFACKPPMKEQEIN
                     ATFQSILNKHYNN"
     gene            3199..4908
                     /locus_tag="O0V33_002279"
     CDS             3199..4908
                     /locus_tag="O0V33_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCX8976893.1"
                     /translation="METGKSDVLDKIEKINKKDSALQEIIPKGYEIEHHQCGIALNQL
                     IPSKKEGEPDKKVFITSTIPQITERFEDIESNEVSFNMLFYDNKTPVNIAVSAEEISD
                     SRQLLKLVNKKLDVTSSTSTKLVDYINASKRYNPPLNVKVATRLGHVKGYFIYPYQEV
                     MKDSNIKLFSNDKGFQKLIDSFQSKGTLEGYSKKVFGQIKDLPMVMVMLYASLGSVLL
                     REFGLQPFIVEISGSTSTGKTFTLNLVSSVWGTSDLITTWSSTQNSIESMASFLNSFP
                     MFKDDTRNTHPKFVTSATYNFSSGESKSRSNINLTLNAKKEWRNILISTGESSIANMA
                     DEKAGVSARVVTLQDPPYPDNFDFTTLDKSFRENYGTLGLAFIKQYESKKDVYKNAFE
                     SYQRYFNQKGSNEIMQRLGRAFALLQVTGEVLNDIDGFEHDHFKIIEQAYDSMVKNNK
                     TIDKPKQLLEELLQYLDANRNNIAGDGYSSVKNGDIKAIYKRDYLCILGQTVHDKLGH
                     EMQTITGQWGKKGYLIKGEKDRLQKKVSHKNIKYRGFAINKEMLEELGFDFSNSHNPY
                     SDY"
     gene            5220..5600
                     /locus_tag="O0V33_002280"
     CDS             5220..5600
                     /locus_tag="O0V33_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976894.1"
                     /translation="MDKEQLKKYIYDYVKEYKEIPIYQLEDLFKEMNHDYIGRTSVTH
                     DKDENIVFWSGWNKITMFALIELVKSEQLDLVYRGSFVMRYLLDGRVPNLPLAICYPE
                     DGQQTDVPSWVPMVLRINKEEKIK"
     gene            5597..6238
                     /locus_tag="O0V33_002281"
     CDS             5597..6238
                     /locus_tag="O0V33_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012990921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCX8976895.1"
                     /translation="MNIETIVNQFETRAGTLLRYYTGLLEHSKVQPCCFKLYNDPFDM
                     AYVMMNGKLFGHVYIKDCKVRQSFELASPKHTEGLIRSIEGHYVGYELHDGKQLSISD
                     MMASQLFEDEYFMYGLQTYAESNNSDVFEYLENGFDTDTLEGIQSSNTDVIANIEMLY
                     QLATGINEPAPELVEGLRLVTEFVQDENATQEDYKALEHKLTELKSSYYSLNK"
BASE COUNT         2466 a          867 c         1245 g         2057 t
ORIGIN      
        1 ttttaaggcc tgcagataaa ctatcaacgt atttttttaa tccgatattt tcatcttcat
       61 ttttaatcgt gaaaataaat ctatgttgtg aattagttat cttttcagtt tctttatatg
      121 agacgtctaa gtcaaaattt aatttttgcc tattatctaa ttcgacgtct actaaaattt
      181 caaagtcaaa attttcaatc gaaaaagcgt gagtaatgtt tgcagttact tcaaatccct
      241 cgaaaacatc attaatttta taattcggtt cttcatcaaa agtaaagtca aaattgatcg
      301 gattatgttc aaaaaattct gaaggagata tgtgcagata actacataat ttatctatag
      361 catcatatct tatcatttca gaatcatttt gtgccattga agtaagtgaa cttcttgcta
      421 tttttacatc ttttgcaaca cgagatattt ttagtcctct ttctgacagt agttcagaca
      481 atctatttct aatcattaca aacctcctaa ttatgttaat aatagcattt ttttggacgt
      541 ttatgtacaa aaaaataaaa aatgattgag aagtcagtcg aaaaactatt gcaaaagaaa
      601 aacgattatg tataataaag ttataaattg attgagaagt cagtcaaaaa cgaaggagga
      661 ttttaattat gactatttta gcgaatacta gaaagtttaa agaagccatg ttcttaaaag
      721 gctttaattt atctgattta tcacgtgaaa caggtgttgg aatttcttat ttaagccaaa
      781 ttattaatgg taaaaagatt ccaagcccta aattagctaa gaaaatggca gaagttttac
      841 aagttgaggt aaatgaatta tttgaatttg aagtaaagga ggcataaacc aatgttcaac
      901 attaatattg atgaagatga agcacgtgag ttacttgagc aggctatcaa tgcacgtgtg
      961 gacgaattag cgaaagagaa atattttatg acttacaaag agttgtctaa ctatctgaat
     1021 ttaagtaagc ctactattga agaattactt attaataatg gcatgaaata ttatatggtc
     1081 ggatctacgt acagattcaa aaagtctgat gtagatgaat tcatggaaca gcttactgct
     1141 catatgaata tccagaataa cgactttaaa caagtcaata tcaaaaagtt attggaggca
     1201 aggcaatgaa aatctactta acttatatct gcttagtttc attgttaaca atattattac
     1261 tagcaatatc taacatgtat gttgctttta gcgtttatgc ttggctaata actttaggat
     1321 gtaatttaac aggagagatt acaacgtgcg aaaacaagtg attattacaa aaacagtagt
     1381 tggctggtac aacattaaag atactcaaca taatttaatg ttaaatatac cgccaaaagt
     1441 atttgaacag tactttcctg atgttagtaa agatgttcaa gttgtgtgtt tagaaatgga
     1501 tttatcaaaa attacagaaa ttaaaaataa gaaaaaagta ggtagttaag atggaaatca
     1561 aacaaaaata tcaattatca aaagtggtta aaatattaga agtagtatta tacgaggaag
     1621 ataagtttca atccgataag gactatcatt atcaggataa agcattatat gaatatgctt
     1681 taaagttagt tcataatgga ttgttcaata ttcttgctga attagatttt gaagatgaag
     1741 catttttaat tcttgatgaa gtaacaatga cgctaagtga tgtcatgaaa gaaacacaac
     1801 acgtttaccg ttatagtgtc atagatgaaa aaggtgaaca caaacataca acagatcgca
     1861 aaggacacgt gattggaatg ttagagtggg cattagatta cattgcggga aatattgaag
     1921 tggaggaatt ataaatgaat tgggaaatta aagatttaat gtgtgatatt gaagcggtaa
     1981 aagaaaaaat caatgatgta gctatcaaac atgcttggtt tgttgaagat agatttgtaa
     2041 aaaatgaatt agaaacaaaa cgggaacata ttaatttttc tgctagctat ttagaacatc
     2101 gtatacaaaa tgaacataca gttgagttat tacatgtgta cttaaaagaa ttcggtgaac
     2161 ttatacaaaa atttcatgaa atagaaaaag catcatctga gaactttggc gaggtatcag
     2221 atgacgcaca aaaattaaaa atcacagagt aatttagaaa ttacacatgt ttattataac
     2281 attttttact ctgtgaatca ctagaggtgc aaaaaatgaa tgaaattaaa ttagaatatg
     2341 acacacatgt ttcagtggta cattatgaaa gtttagactc acgttcattt aatagctttt
     2401 caaaaattaa ttggagtaag ttggttaata aactgtctgt acctatagaa gcaaattata
     2461 agtatgcacg tggtgttgct gtttacggtg atattaaaaa cggtgcaaat gatcaaggtg
     2521 aaattatcaa aaagcatcga aacgataaaa atgtcatata cagagatgtg attgtacttg
     2581 attatgatga aataaatgat ttaaagcaat tacatgaagc aatcagctca gctttaagca
     2641 atgttgcatg gttttggcac acaagttact cgcacagaac tgaacaagct agaatacgcc
     2701 tgtatatccc tctaaatgag cgaataagtg cagatgatta tcgtaaatat acaaaagtat
     2761 tagcaaataa aattggccat aaagtggatg aaggttcata tcagccaagt agatgttttg
     2821 cgttaccagt tattcaaaaa ggacacatat ttattaagcg agtgaatgac tgtccaatta
     2881 tgaatgttga tatgctcgaa cagtggtcga aggagtttga acaatcaaat gctagtccta
     2941 atgtcatagg atacactcga cgcgatagtg agtactggcg cgagctatgc tttggaacaa
     3001 ccgaaggcaa tcgtaacaat gcactagcta gcttaattgg gcatttatta agatgtcacg
     3061 ttaatgatta tattgtttat tcatttgctt tactatgggg gcaattcgca tgtaaaccac
     3121 ctatgaaaga acaagaaatc aacgccactt ttcaatcgat attaaataaa cactataaca
     3181 attagaaagg ggctttgtat ggaaacaggt aaaagtgatg tacttgataa aattgaaaaa
     3241 attaataaaa aagatagtgc cttacaagaa attataccaa aaggttatga aattgaacat
     3301 catcaatgcg gtattgcctt aaatcaactt ataccaagta aaaaagaagg cgagccagat
     3361 aaaaaggttt ttatcacaag tacaatccct caaatcactg aacgctttga agatattgag
     3421 agtaacgaag tcagctttaa tatgcttttc tatgacaata aaacgccagt aaatatagct
     3481 gtgagtgccg aagaaatttc agatagtcgt caactcttga aattggttaa taaaaagctg
     3541 gatgtaacat cgtcgacatc tactaaactt gttgattata ttaatgcatc taaacggtat
     3601 aatccaccat tgaatgttaa agttgcaacg cgtttggggc atgtgaaagg ttattttatt
     3661 tatccttatc aagaagtgat gaaagacagc aatatcaagt tgtttagtaa tgataaagga
     3721 tttcaaaagt taatagactc ttttcaaagc aaaggaacat tagaaggtta ctctaaaaaa
     3781 gtgttcggtc aaataaaaga tctaccaatg gtaatggtta tgttatatgc ctctttaggt
     3841 tcggttttat taagagaatt tggattacag ccctttattg tagaaatatc aggtagtaca
     3901 tctacaggta aaacattcac actcaactta gtatcaagtg tttggggaac gagcgacctt
     3961 atcacgacat ggagttctac tcaaaatagt attgaatcga tggcatcatt tttgaactca
     4021 tttccaatgt ttaaagatga tacacgtaat acacatccta agtttgttac cagtgccaca
     4081 tataactttt ctagtggtga aagtaaatca agaagtaata ttaatttaac actaaacgct
     4141 aaaaaagaat ggcgaaatat tttaatttct actggtgaat catctatcgc aaatatggct
     4201 gatgaaaaag cgggtgtatc agcacgtgta gttacactac aagatccacc atatccagat
     4261 aattttgatt ttaccacatt agacaaatcg tttagggaga actatggaac gttagggttg
     4321 gcatttatta aacaatatga gtctaaaaaa gacgtgtata agaacgcttt tgagagctat
     4381 caacggtatt ttaatcaaaa aggtagtaat gaaatcatgc aacgtttagg acgtgccttt
     4441 gcgttactgc aagttaccgg tgaggttttg aatgatattg atgggtttga acatgaccat
     4501 tttaaaatta tcgaacaagc ctatgacagc atggttaaaa acaataagac gattgataaa
     4561 cctaagcaac tgttagagga actattacaa tatttagatg caaatagaaa taatatcgct
     4621 ggtgatggct atagttcagt caaaaatggt gacatcaaag ctatatataa acgtgattat
     4681 ttatgtatat taggtcaaac tgtacacgat aaattaggtc atgaaatgca gactataaca
     4741 ggtcaatggg gcaaaaaagg atatttaatt aaaggtgaaa aagatcgctt gcaaaaaaag
     4801 gtgagtcaca aaaacattaa gtatagagga tttgctataa acaaagaaat gcttgaagaa
     4861 ttaggatttg atttctcgaa ttctcataat ccttattcag attattaaat agttcccaaa
     4921 gttcccgata agttcccgcg aaaaacatac aaacgggaac tataagacta ctttaaccac
     4981 aagcaattaa agttaatagt tcccgaagtt cccaataaat aatattatta tttattattt
     5041 gaaaacgaac aaatgttgtt agctttatac catatatgat agaaaatttt taacgggtac
     5101 aacgggaact aagtttattt aaagtttata tatcaatggt ttgactagtt cccgataagt
     5161 attttaagtc gggaattcaa cggggactag ttcccattta aaaatattgg aggtaacaca
     5221 tggataaaga gcaacttaaa aagtatatat acgattatgt aaaagaatat aaggagatac
     5281 cgatatatca gttagaagat ttgtttaaag aaatgaatca cgactatata gggagaacca
     5341 gtgtcacaca cgataaggat gagaatattg tgttttggag tggatggaac aaaattacaa
     5401 tgtttgcgct gattgaatta gttaaaagtg aacaacttga tttagtgtat agaggtagtt
     5461 ttgtaatgcg ttatttgttg gatggtagag ttcctaactt accattagca atttgttatc
     5521 cagaagatgg acaacaaacg gacgtgccct catgggtgcc tatggtatta agaataaata
     5581 aagaggagaa aatcaaatga acatagaaac tatcgtaaac caatttgaaa cacgagcagg
     5641 cacgttacta aggtactaca caggattatt agaacatagt aaagtgcaac catgttgctt
     5701 taagttatac aatgatccat ttgatatggc atacgtgatg atgaatggga agttattcgg
     5761 tcatgtatat attaaagatt gtaaagtaag gcaatcattt gaattagcgt cacctaagca
     5821 cactgagggg cttataagaa gtatagaagg tcattatgta ggttatgaat tacatgacgg
     5881 taaacagctt tctattagtg atatgatggc cagtcaatta tttgaagatg agtattttat
     5941 gtatggatta caaacatatg cagaatcaaa taatagtgat gtgtttgagt acctagaaaa
     6001 tggatttgat accgatacac ttgagggcat tcaatcgagt aatactgatg tgatagcgaa
     6061 tattgaaatg ttgtatcagt tagctacagg aatcaatgaa ccagcaccag agttagttga
     6121 ggggttgaga ttagtaactg agtttgtaca agatgagaat gcgacacaag aggattacaa
     6181 ggctttagag cataagttaa ctgagttgaa gtcatcttat tacagtttga ataagtaatt
     6241 aaatatggag tctcacgtgg tgtgtggctc ctaatataaa agtataaggt atagaagttt
     6301 taaaatgtaa aggttgcaac aatagtgagt taatagatag gtaggcgaaa ttcaaaaaag
     6361 tgtgaaatgt tgatattgag ctgttttatg gctttgaaaa taataaggtt atataaaggt
     6421 gttagctttt aacatcggaa ggtatacagt ctttgagaat tgaaaaaatg gcaagatttg
     6481 tgcaaggtgt gagaactttg ttaacgctaa tacaagctaa agtttgtgtt tttggcatag
     6541 gcctaaaagt taagtttgtt cgctgtttgt tcgtgttatt ttatcgaact taagttctat
     6601 attaggttaa tgtgaaaagc ctaacgttaa gttta
//
LOCUS       DAKSQE010000122         6615 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.122, whole genome shotgun sequence.
ACCESSION   DAKSQE010000122 DAKSQE010000000
VERSION     DAKSQE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.122"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(57..548)
                     /gene="thiW"
                     /locus_tag="O0V33_002282"
     CDS             complement(57..548)
                     /gene="thiW"
                     /locus_tag="O0V33_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HCX8976896.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLLTLKKRGILQR
                     FIK"
     gene            complement(575..940)
                     /locus_tag="O0V33_002283"
     CDS             complement(575..940)
                     /locus_tag="O0V33_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HCX8976897.1"
                     /translation="MYNKDDVLKQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            complement(941..2485)
                     /gene="miaB"
                     /locus_tag="O0V33_002284"
     CDS             complement(941..2485)
                     /gene="miaB"
                     /locus_tag="O0V33_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCX8976898.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLDVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFVKEVE
                     PEMVIQ"
     gene            complement(2619..2912)
                     /locus_tag="O0V33_002285"
     CDS             complement(2619..2912)
                     /locus_tag="O0V33_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HCX8976899.1"
                     /translation="MKDTLMSVQIIPKTPDNDNVIPYVDEAIKIIDESGLHFRVGPLE
                     TTVQGNMNECLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            complement(3007..3873)
                     /locus_tag="O0V33_002286"
     CDS             complement(3007..3873)
                     /locus_tag="O0V33_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HCX8976900.1"
                     /translation="MPTFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEQVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQFI"
     gene            complement(3874..5634)
                     /locus_tag="O0V33_002287"
     CDS             complement(3874..5634)
                     /locus_tag="O0V33_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HCX8976901.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLSEIDGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSNGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEEKGKIIATEIKEMV"
     gene            complement(5774..6571)
                     /locus_tag="O0V33_002288"
     CDS             complement(5774..6571)
                     /locus_tag="O0V33_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCX8976902.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IKINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
BASE COUNT         1972 a         1270 c          929 g         2444 t
ORIGIN      
        1 agggcgttca atattaaaat gaacaatgac tctgtttgaa atcatatatc ataaaattat
       61 tttataaacc tttgaagaat accacgtttt tttagagtaa gtaataagaa ataacttata
      121 atagatccga tagcacttga gactatgaac gtaatcatta acggtttaat gaagaagtct
      181 tgaagcccaa ggaaatatgc taatggtata caaattaaac ttccgatgac accagttcca
      241 agtacttcac cgaccgcggc catgaatata tgtttacgat atatgtaaaa catactagcc
      301 aataaaactc caatcatact acccggaaat gcaaaagctg taccagttcc aaaaagaact
      361 cttaaaattg atgatataag cgcttgagct aatccatacc aaggacctac tatgaccgca
      421 cttaatacat ttacaaaatg ctgtactggt gctgccttaa ctggtcctag aggaatgatg
      481 ataatactgc ttaatacaac atttattgca attaaaagtg cagttatagc cagttttctt
      541 gatttcatat gattgttctc ctttttgttt gtaattaatc actatgtttg gctttattat
      601 ggtcatttaa acgtgtttcc attgttgata caaacatttt caataattga ttcgcttcat
      661 attgtgaagt ttgaaactgt tcaactatgg gcaatgtatt tatttctgct tctatacttt
      721 gaatggtatg ttccgactgt tctagcgcat tttgtttccc gtaattttga aagtttactg
      781 cttgtttttg atgctttttt aacatatcca ttttagtctt tatcgtttgg ttctgatgaa
      841 tctgtgcttc aatttgttga tatgttttga tagtatccaa atttttaatt ttatttgcaa
      901 tattatccgc ttgtttcaac acgtcatctt tattatacat ttattgaatc accatttccg
      961 gctctacttc ctttacaaag ctgccattta atgaatactg tttagcttca tctattcgta
     1021 cttccactag tttaccaatc atttctttag gcgctttgaa attaactaac ttatttttat
     1081 cagtgtagcc agcaagaacc tgatcatctt ttttactact accttcacaa agtactgtta
     1141 cagtttgtcc ttcgtactta ctcatagcta tttgtgaata atgaccaact tttttattca
     1201 aacgttgcaa tcgttccttt ttgacatcta aaggtacatt atctttcatt ttagcagcag
     1261 gcgtaccatc acgttgtgaa tacaaatacg tatatgcatg ttcaaaacca acttcatcat
     1321 acaaagttaa agtttcttca aattgttcct ctgattcatt tggataccct acaataatat
     1381 ctgtagttaa tgctacattt ggaattctat ctttgattcg ttttactaaa tccaaataac
     1441 tttctcgtgt atattttcta cccattattt ttaatactgc attatttcca gattgaactg
     1501 gcaagtggat atgaggaacg atattaccac cctctgaaat aacatcaatc atgtgatctg
     1561 tgaagtccca aggatgactt gttgtgaaac gaactcttgg aatcgctatt ttagaaattg
     1621 cttgtaaaag gtctcctaag tcatattcta tatcctgtaa atctttacca taagaattta
     1681 cattttgacc taaaagcgtt atttctttgt aaccttcacg agcaagttca cgtacttcat
     1741 ctataatgtc ttcaggtcta cggcttcgtt ctttgcctct tgtaaatgga acaatacaat
     1801 atgtacaaaa cttatcacaa ccatacataa tattgaccca tgctttaatg ttgccttcac
     1861 ggacttttgg aagattttca ataacgtctc cttctttaga ccatacttca acaaccattg
     1921 ctttagataa gtatgcttct tctaaaattt ctggtaaatg atgaatattg tgtgtaccaa
     1981 atatcatatc tacattttga tacgatttta aaattttatt cactactgac tcttcttgtg
     2041 acatacaacc acaaactccg attaaaatat caggtcgttc tttcttcaaa tgcttcaaat
     2101 tacctatttc actaaaaact ttgttctcgg cattttctct aatcgcacat gtattaatta
     2161 aaataacatc tgcagtgtta atatcagtcg ttgcttgata gcctaatgct tcaagtatac
     2221 cagcaatgac ctcagtgtca tgtgcattca tttgacatcc atatgtttta attaaaaatg
     2281 tacgctcgtt ccccataccg cgatattttt catcaatttg gaaatctcta ttataacgaa
     2341 cttcttgttt acctcttttt ttcgcttctt ttaaattagg cggttgataa acatgttcaa
     2401 aatatttact ataatctttt tctgctttct tatctctctc agctaaaaca tctacagaac
     2461 ttgcttttct ttgttcttcg ttcactttac aaaatccttt ctatttgccc gtatcattta
     2521 ataattatat taaatttaac tataaagtgc aaaaacagtt aaagttatac gtcatctcta
     2581 acaagccaat tcaaatccag tttacttcac ttttaatgtt attcatcata tttttcagtt
     2641 aaagtttcaa tagtaatgcc gtctggcaca tgataaaact taacttggct aataatactt
     2701 ggacattcaa gttccaccat tcgttcattt aacgattgta ttaaaattaa acattcattc
     2761 atatttcctt gtactgtcgt ttctaacgga cctactctaa aatgcaaacc agattcgtca
     2821 attattttaa tcgcctcgtc tacgtaaggt ataacattgt cattgtctgg tgttttagga
     2881 attatttgta cactcattaa tgtgtctttc atttttaaaa actcctttat attttgtaat
     2941 agtaaaaaaa ctgagtagca taaatggatc tgttataaat acatttatac gactcagttt
     3001 taattattaa ataaattgtt cagttaatgc attgaacgtg tcttcagtaa ttttgatatc
     3061 tcttttagct agtggtgtat catctaactc tttgatttga gattcatatg atggggtttc
     3121 tttatcttga taaacaatac cagttactaa agattcatgt tcaataacag ttttagtcgc
     3181 taattgttta tctgtagaat catagttttc aatgtcatca acacttgtta aatgttcttt
     3241 aaaccaatcg tatgtgttaa ttttattata agtcacacat ggtgaaaaga cattaacaaa
     3301 tgaaaaccca tcgtgattaa ttgcatcttc aattagtttt gttaatcctt taatatcact
     3361 tgaaaaacct tgggctacaa atgtggcacc agatgataat gctaattcta aaggcgcaac
     3421 atttttttct atattacctt ttggcgttgt tttagtaaca aatcctactg ctgatgacgg
     3481 cgatgtttgt ccctttgtca aaccataaat ttgattatcc atgactatat acgtcatgtt
     3541 catatttctt cttaaagcat ggattgtatg ccccatacca atagcataac catcaccatc
     3601 tcctccagat gcaataacag ttaaatcttt attcgccatt tttacacctt gagctaaagg
     3661 taatgcacgt ccgtgaatag aatgaacgcc ataagaatta atatatcctg aaagacggcc
     3721 agaacatcct ataccggtga tgatagctac ttgttcaggt tctaacccta tatttgcggc
     3781 tgctttttga attgcagctt gtactgagaa atcgccacat ccagggcacc agttaggctt
     3841 aacattattt ctaaaatctt taaatgtcgg catctatacc atctccttta tttcagtagc
     3901 aattatttta cctttttctt cgatttcatg tggtaggaaa ggtgtcccat catactttgt
     3961 ataattttca atcttatcat gaatattgac attcattttt ataatactag ccaattgtcc
     4021 ttgataattg tgctccacta caacgacttt cttcgcttta ttaactgcat cttgaataac
     4081 gcttgttggg aatggatgca attgtctaat ttgtatagtg ttaactttta tgccttgttg
     4141 attcaaacga ttactacctt cttgaattgc accttttgta gaaataaaac cgatataaag
     4201 aatatctgca tcttcatgtt gtaagttagc ttctactggc gattcaatta gtaactgctc
     4261 aattttacgc attcgttttt ccatttgttg ttgtctattt gacgcagatt cgctaggttt
     4321 accttcttca ttgtgttcca caccagttat atgatgaata cctcctttaa cacccgggat
     4381 aggtctagga gaaacaccat tggatgttaa cgcataacgc ttgaaataac ctttatcatc
     4441 ttcttcacgt tcaatatcag attgaatgat ttcaccacgt ttaatttcaa tgcgattata
     4501 atctaatttt tcaacagttt gttttcctaa agataattgt aaatcactta gaactataac
     4561 agggcattga tattgttctg ctaaattaaa ggcttccata gttaaataaa atgcatcttc
     4621 tgcatcagta ggtgctacaa ctatttttgg aatatcacca tgtgtaccat aaatcatttg
     4681 cattaaatct gactgttctt gtttcgtagg taatccagta gaaggtccac ctcgttgggt
     4741 attaattatg actaatggcg tttcagtcat accagataat ccaattgctt ccatcattaa
     4801 agataaacct ggaccagcac tagccgtaaa cgctctgact ccaccataat ttgcaccaat
     4861 agccatagtt acagcagcaa tttcatcttc tgtttgaata accgctccgt ttactttaga
     4921 tatattggca atcatatatt ccataacttc agacgcaggt gtaataggat atgccgccat
     4981 aaattgtgaa cctgcagcaa ttgcacctaa tccaatggca tcgttaccaa tcatatatag
     5041 atgtggtagt gcatctgttg actctaattc aaagtccccg tcgatttcag ataagcgaga
     5101 ttgcattaat tgataacctt cgtttaatgc ttggatattg acttcaacta ccttgtcacc
     5161 ttttttagaa aacatattag taataagttc ttcaaatgta tttgtattca aattcatcaa
     5221 tgcgctagta gcacctattg caaccatgtt tttcattaat gctgtaccta attctttagc
     5281 ggttgctgta aaaggtaatt caataagctg tgcatgacat ccttctggtt tcacaggttt
     5341 agccttggca tcagctaaaa tgatactgtc ttctctcatt tcatgatggt taacatcaat
     5401 tgtttcttgg tcaaatgcaa tcaaaatatc taaatcatca ctaattgcat gaacaggcgt
     5461 cgtagaaact ctaattttat tattcgtatg tccacctttg atacgacttg aaaaatgtct
     5521 atatccatat aaataatatc cttttctatt catagccgtt gcgaagattt ccccagttga
     5581 ttcaatacct tcgccttgtt gaccgccaac tttccacgat aattgtggtt tcatagttat
     5641 atgcctccta tggatatatt tcatattaaa tgataacaaa aagaaccacc gatttactat
     5701 gtaaacagtg gtactatatt aaattcatac taatggacga tagggtccaa ttagaatgtt
     5761 tacttacgta attttaaaat gttgaaaatg gatggtcatc atttatcaat atacgttcga
     5821 tgtgctttgt tttaccttct ttgtccaaat caataacaac accagataat acacttctac
     5881 cttcatttgg aacaacatgt ctttgtggca aactagtgat aaaacgctca attacctctg
     5941 ttttatttat tcctaaaatg ccgtcataaa agcctgtcat accaacatcc gttatatacc
     6001 ctgtaccctt tggtaaaata cgttcatctg ctgtttgaat gtgtgtatgc gttccaacaa
     6061 cagcgctagc tctaccatct aaatgccatc ccattgcata cttttcagaa gttgtttctg
     6121 catgaaaatc aacaaatata aacggagttt gttcttgtgc ttccttgact aattgatctg
     6181 cctttttaaa aggatcatca atatctggca taaacgctct tccttgcaga ttaataactg
     6241 caagtttaat atcattaatt tttataaatc tcataccaat tcccggcgct tcatccggaa
     6301 aattagctgg tcttactagt cgttttgctt catctataaa atcataaatt tcacgttgac
     6361 catatgtgtg attacccata gtcatgaaat ctacaccatt tcttagtaat tgtttatata
     6421 ttttttcagt caaaccttta ccatgcgctg cattttcagc atttacaatt gtaactgttg
     6481 gtttatactt ttgcttcagt tgaggtatgt acgtttcaat tgcgtctcgt ccaattttac
     6541 ctacgatatc ccctataaac attattctca tgttttgttc atcctctcat aaatactagt
     6601 ttaattcata cgttc
//
LOCUS       DAKSQE010000123         6586 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.123, whole genome shotgun sequence.
ACCESSION   DAKSQE010000123 DAKSQE010000000
VERSION     DAKSQE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6586
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.123"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            37..486
                     /locus_tag="O0V33_002289"
     CDS             37..486
                     /locus_tag="O0V33_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCX8976903.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFKHYCEHEVLSSIINGAYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNEMAEYVYAKLFR"
     gene            835..2070
                     /gene="arcA"
                     /locus_tag="O0V33_002290"
     CDS             835..2070
                     /gene="arcA"
                     /locus_tag="O0V33_002290"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCX8976904.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKEVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            2103..3113
                     /gene="argF"
                     /locus_tag="O0V33_002291"
     CDS             2103..3113
                     /gene="argF"
                     /locus_tag="O0V33_002291"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCX8976905.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSVM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            3204..4634
                     /gene="arcD"
                     /locus_tag="O0V33_002292"
     CDS             3204..4634
                     /gene="arcD"
                     /locus_tag="O0V33_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCX8976906.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPVFKGGNTLPSIIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFGMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYSIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            4651..5592
                     /gene="arcC"
                     /locus_tag="O0V33_002293"
     CDS             4651..5592
                     /gene="arcC"
                     /locus_tag="O0V33_002293"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCX8976907.1"
                     /translation="MKEKIVIALGGNAIQTKEATAEAQQTAIRRAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFDNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFAEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            5691..6395
                     /locus_tag="O0V33_002294"
     CDS             5691..6395
                     /locus_tag="O0V33_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCX8976908.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLLCHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
BASE COUNT         2358 a          902 c         1274 g         2052 t
ORIGIN      
        1 aatacaagaa acgtaactac caaaggagtt tacaatatga agaaaagtaa acgattagaa
       61 attgtttcta caatagttaa aaagcataag atttataaaa aagaacaaat catttcatat
      121 attgaagaat attttggtgt aagatatagt gcaacaacaa ttgctaaaga cttgaaagaa
      181 cttaatatat atcgtgtccc tatcgattgt gagacatgga tttataaagc tattaataat
      241 caaacagaac aagagatgag agaaaagttt aaacattatt gtgaacatga agtacttagc
      301 tcgattatca atggcgcata tattatagtc aaaacctcgc caggtttcgc tcaaggcatt
      361 aactacttta tcgatcaatt aaatattgaa gaaatattag gtacggtgag cggaaatgat
      421 actacattaa ttttaacagc ttcgaatgaa atggcagaat atgtatatgc aaagttattt
      481 agatagtcct gttttaaatg tatattaaca aaccttaata atgtcgtgcg tacacaagtt
      541 gattactgaa atattaactt gtgtacgcgt ttttttatga aaagaaagaa ttcatagtca
      601 ttcatttgac tgtataaaaa actttataca acatgttttt atgggtattt ttgaataaaa
      661 aatgtatatt ttgacccaaa atacctttat ttatgtataa aaatccatta ttatgtattg
      721 tataacaaaa agatatgaaa ttttcgactt tctttatgtg aatataatca catgtaagcg
      781 tttgaagatt gtctatactc taaatgaatt caaagataaa aggaggaaat agacatgaca
      841 gatggtccaa ttaaagtaaa tagcgaaatt ggagctttaa aaactgtgtt acttaagcgt
      901 cctggaaaag aattagaaaa tttagtacct gattatttag atggattact atttgatgat
      961 attccatatt tagaagtagc tcaaaaagag catgaccatt ttgcacaggt gctaagagaa
     1021 gagggtgttg aagtacttta ccttgagaag ttagcagctg aaagtattga aaatcctcaa
     1081 gtaagaagtg aatttattga tgatgtatta gcagagtcta aaaaaacaat attaggtcat
     1141 gaagaagaaa ttaaggcatt atttgcgaca ctttctaatc aagaacttgt agataaaata
     1201 atgtcagggg tacgtaagga agaaattaat ccgaaatgta cacatctagt agagtatatg
     1261 gatgataagt atccattcta tttagatcca atgccaaacc tttattttac tagagatcca
     1321 caagcctcaa taggacacgg tataacaatc aatcggatgt tctggagagc acgacgacga
     1381 gaatcaatat ttattcaata tattgtaaag catcatccta gatttaaaga tgcgaatatt
     1441 ccaatttggt tagatcgaga ttgcccattc aatattgaag gtggcgatga acttgtttta
     1501 tctaaagaag tcttggctat aggcgtttca gaacgtacat ctgcacaagc tattgaaaag
     1561 ttagcgcgac gtatttttga aaatccgcag gcgacgttta aaaaagtagt agcaattgaa
     1621 attccgacta gtcgaacatt tatgcactta gatacagtat ttacaatgat agattatgac
     1681 aaatttacaa tgcattcagc cattttaaag gcagaaggca atatgaatat atttattatt
     1741 gaatatgatg acgtaaataa agatattgcc atcaaacaat ctagtcattt aaaagatact
     1801 ttagaagacg tactaggtat agatgatatc caattcattc caacaggaaa tggtgatgtc
     1861 attgatggtg ctagagagca atggaatgat ggctcaaata cattatgtat aagaccaggt
     1921 gttgtagtga cttacgatag aaactatgta tcgaatgatt tattgagaca aaaaggcata
     1981 aaagtcattg aaatatctgg tagcgagttg gtgcgtggac gtgggggtcc tagatgtatg
     2041 agtcaaccac tattcagaga agacatttaa cgtaataaat tatagaagcg agggtgaaaa
     2101 taatgacaga aattcaaaaa ccgtatgatt taaaaggcag atcattatta aaagaaagtg
     2161 attttaccaa agcagaattc gaaggactta ttgattttgc aattacatta aaagagtata
     2221 agaaaaacgg tattaagcat cactacttat ctggaaaaaa tattgcacta ctattcgaaa
     2281 agaattcgac gagaacacgt gcagcgttta cagttgcgtc tattgattta ggtgcgcatc
     2341 cagaattttt agggaaaaat gatattcaat taggcaaaaa agaatctgta gaggatactg
     2401 cgaaagtatt aggtagaatg ttcgatggta ttgaattccg tggtttttca caacaagctg
     2461 ttgaagattt agcgaagttc tctggtgtac cggtgtggaa tggattaaca gacgattggc
     2521 atcctacaca aatgttagct gattttatga caataaaaga gaattttgga tatctagaag
     2581 gaataaactt aacttacgtt ggagatggac gtaataatat tgcgcattca ttaatggtag
     2641 caggtgctat gttaggtgtt aatgtaagaa tttgtacacc taaatcatta aatccaaaag
     2701 aggcatatgt tgatattgca aaagaaaaag cgagtcaata tggtggttcg gtcatgatta
     2761 cggataatat tgcagaagca gttgaaaata cagatgctat atatacagat gtttgggtat
     2821 cgatgggtga agaaagtgaa tttgaacaac gtattaattt attaaaagac tatcaagtga
     2881 atcaacagat gtttgattta acaggtaaag attcaacgat attcttacat tgtttaccag
     2941 cattccatga tacaaataca ctttatggac aagaaattta tgaaaaatat ggattagctg
     3001 aaatggaagt tacagaccaa atctttagaa gtgaacattc aaaagtgttt gatcaagctg
     3061 aaaatagaat gcatacaatt aaagcagtaa tggcagcaac attggggagt taatcactaa
     3121 atggaacgat atgaatatga tgtgtctgat gatataagtg tcatgtacag acacctcata
     3181 ttgatattaa aggagaaatg aatatgaacg aatcaggaga taacaaactc agtaaatctt
     3241 ctttaattgg actagttata ggatccatga ttggtggcgg tgcgttcaat ataatgtctg
     3301 atatgggcgg taaagccggt ggattagcca ttattattgg ttggattatt acagctatag
     3361 gaatgatttc attagcgttc gtatttcaaa atttaaccaa tgaacggccg gagctagacg
     3421 gtggtattta tagttatgct caagcaggat ttggcgattt tgtaggcttt atcagtgctt
     3481 ggggatattg gttctcagcg tttttaggca atgttgccta tgcaacacta ttgatgtcag
     3541 cagtaggtaa ctttttcccg gtatttaaag gaggcaacac attacctagt attattgtcg
     3601 cctcgttact actctggggt gtccatttct tgattttaaa aggcgttgaa acagcagcat
     3661 ttatcaatag tattgttact gttgcaaagt taataccgat tttacttgta atcatatgca
     3721 tgataattgc attcaatttt gacactttta aaacaggctt tttcggtatg acgtcagagg
     3781 gtgtattgcc atttagttgg gcgagcacaa tgagccaagt taaaagtacg atgctagtga
     3841 cagtttgggt gtttatcggt atcgaaggtg cagtaatttt ttctagtaga gctaaaaata
     3901 aaaaagatgt aggtagtgct acggttatag gacttatatc agttttaatt atctatttct
     3961 tattaactgt attagctcaa ggcgtgattt tgcaaaatca tatttctcaa ttagattcgc
     4021 caagtatggc acaggtgctt gcaactattg taggtggttg gggatctaca cttgtaaata
     4081 ttggtttaat tatttcggta ctaggtgcat ggttaggatg gacattgctt gctggtgaat
     4141 tacctttcat tgttgcaaaa gatggattat ttccgaaatg gtttgctaaa gaaaataaaa
     4201 atggcgcacc tgtaaatgca ctgcttatta ccaatatatt agtacaatta tttttaataa
     4261 gtatgctatt tacacagagt gcgtatcaat ttgcattttc actagcatca agtgctattt
     4321 tataccctta catgttcagt gcattttacc aagttaaata cacgttagag caccgacagc
     4381 aagcaactac taaacaatgg acgattggta tcatagcctc aatttatgct atatggctta
     4441 tatatgcagc aggtatcaat tacttattat tgacgatgtt actttatatt ccagctcttc
     4501 ttgtttattc aatcgttcaa aagaataatc agacacgttt gattaaatca gactatattc
     4561 tttttatgat tattatcgta cttgcagtta tcgggttaat taagttattg atgggaacga
     4621 ttaatgtttt ttaaaaggag cgacaaaaat atgaaagaga aaattgtcat tgcattaggc
     4681 ggtaatgcga tacagacaaa agaagcaaca gctgaagcac aacaaacagc tattagacgt
     4741 gcgatgcaaa accttaaacc tttatttgat tcaccagcgc gtattgtcat ttcacatggt
     4801 aatggcccac aaattggaag tttattaatc caacaagcta aatcgaacag tgacacaacg
     4861 ccggcaatgc cattggatac ttgtggtgca atgtcacagg gtatgatagg ctattggttg
     4921 gaaactgaaa tcaatcgcat tttaactgaa atgaatagtg atagaactgt aggcacaatc
     4981 gtaacacgtg tggaagtaga taaagatgat ccacgatttg ataacccaac taaaccaatt
     5041 ggtccttttt atacgaaaga agaagttgaa gaattacaaa aagaacagcc agactcagtc
     5101 tttaaagaag atgcaggacg tggttataga aaagtagttg cgtcaccact acctcaatct
     5161 atactagaac accagttaat tcgaacttta gcagacggta aaaatattgt cattgcatgc
     5221 ggtggtggcg gtattccagt tataaaaaaa gaaaatacct atgaaggtgt tgaagcggtt
     5281 atagataaag attttgctag tgagaaatta gcaacgctga ttgaagcaga taccttaatg
     5341 attcttacga atgtagaaaa tgtatttatt aactttaatg aacctaatca acaacaaatc
     5401 gatgatattg atgtagcaac actgaaaaaa tacgcggcac aaggtaagtt tgcggaagga
     5461 tcgatgttgc caaaaataga agctgcgata cgatttgttg aaagtgggga aaacaaaaaa
     5521 gttatcatta ccaatttaga gcaggcatac gaagctttga ttggtaataa aggtacgcac
     5581 attcacatgt agcactttat cacgcgacaa aacattaaat atgtttctcc gttgattcaa
     5641 atgaaaaagt tgtctgctga cactttgcaa ggtttgaagg agtttaactt atgacagaaa
     5701 actttatttt gggtagaaat aataaattag aacatgaact aaaggcatta gcagattaca
     5761 ttaatatccc atatagtata ttacaaccat atcaaagtga atgttttgtc agacattata
     5821 caaaaggcca agttatttat ttttcgccac aagaaagtag caatatttac tttttaattg
     5881 aaggtaacat tattagagaa cattacaatc aaaatggaga tgtatatcgt tattttaata
     5941 aagagcaagt attatttcca atcagtaact tatttcatcc gaaagaggtt aacgaattgt
     6001 gtacagcatt aaccgattgt acagttcttg gattgcctag agaattgatg gcctttttgt
     6061 gcaaagctaa tgatgatata tttttgacac tttttgcatt aataaatgat aatgagcagc
     6121 aacacatgaa ctataacatg gcattaacaa gtaaatttgc taaagatcga attatcaaat
     6181 tgttatgcca tctatgtcag acagtaggat acgatcaaga tgaattttat gaaatcaaac
     6241 agtttttaac tattcaactc atgagtgata tggctgggat ttcccgggaa acagctggtc
     6301 atattattca tgaacttaaa gatgaaaaac ttgttgttaa agatcataaa aattggttag
     6361 taagcaaaca tttattcaat gatgtatgtg tttaatatac aatgtaaaat gaataagttg
     6421 aacataaggt ctaacgtaca tttatacgtt aggccttttt tgctagcatg atgattaatt
     6481 taatatgtta gttaaatttg attgttgaaa ttacagtaaa atttaaggtg atgaaaaatt
     6541 tagaacttct aagtttttga aaagtaaaac atttgcaata gtgtaa
//
LOCUS       DAKSQE010000124         6575 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.124, whole genome shotgun sequence.
ACCESSION   DAKSQE010000124 DAKSQE010000000
VERSION     DAKSQE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.124"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            124..1353
                     /locus_tag="O0V33_002295"
     CDS             124..1353
                     /locus_tag="O0V33_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCX8976909.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTMAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKLTDLQ"
     gene            1548..2405
                     /locus_tag="O0V33_002296"
     CDS             1548..2405
                     /locus_tag="O0V33_002296"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCX8976910.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEI
                     LKIHQEVTK"
     gene            2405..3349
                     /locus_tag="O0V33_002297"
     CDS             2405..3349
                     /locus_tag="O0V33_002297"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HCX8976911.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQA
                     LAIKSAFVEQLDSLESLSRDEIANDRFEKFRNIGSYIE"
     gene            3618..4586
                     /gene="pfkA"
                     /locus_tag="O0V33_002298"
     CDS             3618..4586
                     /gene="pfkA"
                     /locus_tag="O0V33_002298"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCX8976912.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQFINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            4608..6365
                     /gene="pyk"
                     /locus_tag="O0V33_002299"
     CDS             4608..6365
                     /gene="pyk"
                     /locus_tag="O0V33_002299"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCX8976913.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIEEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     ETAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVTNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNV
                     LVTIDAAQGKIFEGYANVL"
BASE COUNT         2338 a          885 c         1338 g         2014 t
ORIGIN      
        1 ttacataatt aaaactataa gttcttactc attcagatat acccaatatt tttactttta
       61 taatgtaatg cgttttatcc aagttatttt ttaaaaataa atattgaatt aggggctgat
      121 ttcatgtcat taagagatga agcattggaa atgcacaaac gtaatcaagg taaattagaa
      181 gttaaaccaa atgtaaaagt tactaataaa gaagaattaa gtttagcata ctcacctggc
      241 gttgctgaac cgtgtaaaga tatatatgaa gacaaaagaa aagtatatga ttacacaatt
      301 aaaggaaata cagttgcagt tattactgat ggaacagcgg tattaggttt aggtaacatt
      361 ggacctgaag caagtattcc tgtaatggaa ggtaaagcag tattattcaa aagcttcgct
      421 ggtatcaatg gggtgcctat tgcgttaaat acaactgata ccgaagaaat cattaaaaca
      481 gttaaattgt tagaacctaa ttatggtggt attaatttag aggatatttc ggcaccacgt
      541 tgttttgaaa ttgaagaacg attgaaaaaa gaaactaata ttccggtatt ccatgacgat
      601 caacatggta cagcaattgt aacaatggca ggtttggtaa atgcattgag agttgttaac
      661 aaagatattg ctaaaataaa agttgtacta aatggtgctg gtgcagcagg aatagccatt
      721 gttaaattac tatacgcgta tggtgtaaga aatatggtta tgtgtgactc aagaggcgca
      781 atttttgaag gacgttcata tggtatgaat cctacgaaag atgttgtagc taagtggaca
      841 aataaagaca agattgaagg gtctctagaa gaagtcgtaa aagacgcaga tgtatttatt
      901 ggagtttctg tagctaatgc gctgtcacaa gatatggtta agagtatggc agataatcca
      961 attatatttg caatggctaa tccaaatcct gaaataatac ctgatgatgc caaagcagca
     1021 ggtgcacgcg ttgttggtac aggacgttca gactatccta accaaattaa taatgtatta
     1081 gctttccctg gtatttttag aggtgcatta gaggttgaag ctacacatat aaatgaagaa
     1141 atgaaaaaag cagctgtaga agcgattgct gatttaattg atagttctga attaaatgaa
     1201 gactactgta tcccaggacc gtttgataaa cgtgtagcgc catcagttgc tcgtaatgtt
     1261 gctaaagcgg caatggaatc tggagtagct aggattgaag ttgatccgca agatgtgtat
     1321 gataaaacaa tgaaacttac agatttacaa taaaaataat taacgtttaa ttaaagtgct
     1381 taaaagttag caataaagag tcattaaaat gatgaaaatg aaagtttatg ataaacattc
     1441 aacagtcaaa cgaatataaa tcaaataaat ttaaacccgt ttttaactgg tcaagttcag
     1501 tttaaggctc taaatggtta gaacagaggt tatttggagg ttttcctatg tttaaagatt
     1561 tttttaatcg aacaaagaaa aagaaatatc ttacagtaca agactctaaa aataatgatg
     1621 tgcctgcagg tattatgact aagtgtccaa agtgtaagaa aattatgtac acaaaagaat
     1681 tagctgaaaa tttaaatgtg tgctttaatt gtgatcatca tattgcttta actgcgtata
     1741 aacgtataga agcaatttct gatgaaggat catttacaga attcgataag ggaatgacct
     1801 ctgcgaatcc attagatttt ccaagttatt tagaaaaaat tgaaaaggac caacaaaaga
     1861 caggtcttaa agaagcagta gtgactggta cagcgcaact agatggtatg aaatttggcg
     1921 ttgctgtcat ggattcacgt tttagaatgg gaagtatggg atcggttatt ggtgaaaaga
     1981 tatgtcgcat cattgattac tgcactgaga accgtttacc atttatactt ttctctgcaa
     2041 gtggtggtgc acgtatgcaa gaaggtatta tttccttgat gcaaatgggt aaaaccagtg
     2101 tatctttaaa acgtcattct gacgctggac tattatatat atcatattta acacatccaa
     2161 ctactggtgg tgtatctgca agttttgcat cagttggtga tataaatcta agtgagccaa
     2221 aagcattgat aggttttgca ggtcgtcgag ttattgaaca gacaataaac gaaaaattgc
     2281 cagatgattt ccaaactgca gaatttttat tagagcatgg acaattggat aaagttgtac
     2341 atcgtaatga tatgcgtcaa acattgtctg aaattctaaa aatccatcaa gaggtgacta
     2401 aataatgtta gattttgaaa aaccactttt tgaaattcga aataaaattg aatctttaaa
     2461 agaatctcaa gataaaaatg atgtggattt acaagaagaa attgacatgc ttgaagcgtc
     2521 attggaacga gaaactaaaa aaatatatac aaatctaaaa ccatgggatc gtgtgcaaat
     2581 tgcgcgtttg caagaaagac ctacgacact agattatatt ccatatatct ttgattcgtt
     2641 tatggaacta catggtgatc gtaattttag agatgatcca gcaatgattg gtggtattgg
     2701 ctttttaaat ggtcgtgctg ttacggttat tggacaacaa cgtggaaaag atacaaaaga
     2761 taatatttat cgaaattttg gtatggctca tccagaaggt tatcgaaaag cactacgttt
     2821 aatgaaacaa gctgaaaaat tcaatcgtcc tatctttaca tttatagata caaaaggtgc
     2881 atatcctggt aaagctgctg aagaacgtgg acaaagtgaa tctattgcaa caaatttgat
     2941 tgagatggct tcactaaaag taccagttat tgcgattgtc attggtgaag gtggcagtgg
     3001 aggtgcccta ggtattggta ttgccaataa agtattgatg ttagagaata gtacctactc
     3061 tgttatatct cctgaaggtg cagcggcatt attatggaaa gacagtaatt tggctaaaat
     3121 tgcagctgaa acaatgaaaa ttactgccca tgatattaag caattaggta ttatagatga
     3181 tgtcatttct gaaccacttg gcggtgcaca taaagatatt gaacaacaag ctttagctat
     3241 taaatcagcg tttgttgaac aattagattc acttgagtca ttatcacgtg atgaaattgc
     3301 taatgatcgg tttgaaaaat tcagaaatat cggttcttat atagaataat caacttaagc
     3361 atttttatgt taaatcgtta ctgggtttta ccataaattg aagtacatta aaacaataat
     3421 tcaatattta gatactgaat tttaactaag attagtagtc aaaattgtgg ctactaatct
     3481 ttttttaatt aagttaaaat aaaattcaat atttaaaacg tttacatcaa ttcaatacat
     3541 tagttttgat ggaatgacat atcaatttgt ggtaatttag agttaaagat aaatcagtta
     3601 tagaaaggta tgtcgtcatg aagaaaattg cagttttaac tagtggtgga gattcacctg
     3661 gaatgaatgc tgccgtaaga gcagttgttc gtacagcaat ttacaatgaa attgaagttt
     3721 atggtgtgta tcatggttac caaggattgt taaatgatga tattcataaa cttgaattag
     3781 gatcagttgg ggatacgatt cagcgtggag gtacattctt gtattcagca agatgtccag
     3841 agtttaagga gcaagaagta cgtaaagttg caatcgaaaa cttacgtaaa agagggattg
     3901 agggccttgt agttattggt ggtgacggta gttatcgcgg tgcacaacgc atcagtgagg
     3961 aatgcaaaga aattcaaact atcggtattc ctggtacgat tgacaatgat atcaatggta
     4021 ctgattttac aattggattt gacacagcat taaatacgat tattggctta gtcgacaaaa
     4081 ttagagatac tgcgtcaagt cacgcacgaa catttatcat tgaagcaatg ggccgtgatt
     4141 gtggagatct agcattatgg gctggattat cagttggtgc tgagacaatt gtagttccag
     4201 aagtgaaaac agatattaaa gaaatagctg ataaaattga acaaggtatt aaacgtggta
     4261 agaaacactc aatcgttctt gtagcagaag gttgtatgac tgcgcaagat tgtcaaaaag
     4321 aattatcaca attcatcaat gttgataata gagtgtctgt gttaggtcac gttcaacgtg
     4381 gtggtagccc aacaggtgcg gatagagttt tagcatcacg attaggtgga tatgcggtag
     4441 acttattaat gcaaggtgaa acagctaaag gtgttggaat taagaacaat aaaattgtag
     4501 caacatcttt tgatgaaatt tttgatggta aagatcataa atttgattat agtctatatg
     4561 aacttgctaa caagttatct atataagatt tcaggaggaa ttataaaatg agaaaaacta
     4621 aaattgtatg tacaattgga ccagcttcag aatcagaaga aatgattgag aaattaatca
     4681 atgctggtat gaacgttgca cgattaaact tttcacatgg tagtcatgaa gagcataaag
     4741 gtagaattga tacaattcgt aaagtagcta aaagattaga caaaattgta gcaattttat
     4801 tagatacgaa aggtccagaa attcgtacgc ataatatgaa agacggtatc attgaacttg
     4861 aacgtggtaa cgaagttatt gttagcatga atgaagttga aggaacacct gaaaagttct
     4921 ctgtaactta tgaaaactta attaacgatg ttcaagtagg ttcatacatt ttacttgatg
     4981 atggcttaat tgaattacaa gttaaagata ttgaccatgc taaaaaagaa gttaaatgtg
     5041 atattttaaa ctctggtgag cttaaaaaca aaaaaggtgt taacttacct ggtgtaagag
     5101 ttagtttacc tggtattaca gaaaaagatg ctgaagatat ccgtttcggt attaaagaaa
     5161 atgttgactt cattgcagca agtttcgtac gtcgtcctag tgatgtttta gaaattcgtg
     5221 aaattttaga agaacaaaaa gctaacattt cagtattccc taaaattgaa aaccaagaag
     5281 gtattgataa tattgaggaa attcttgaag tgtctgatgg tttaatggtt gcacgtggtg
     5341 acatgggtgt tgaaatccca cctgaaaaag taccaatggt tcaaaaagat ttaatcagac
     5401 aatgtaacaa attaggtaaa ccagttatta cagctacaca aatgttagat tctatgcaac
     5461 gtaacccacg tgctacacgt gcagaagcta gtgacgttgc caacgcaatc tatgatggta
     5521 cagatgcagt aatgttatct ggtgaaactg ctgctggttt atatcctgaa gaagctgtta
     5581 aaacaatgag aaatattgct gtatcagctg aaacagccca agattacaaa aagttattgt
     5641 cagatcgtac taaattagtt gaaacttcat tagtgaatgc tatcggtatt tcggttgcac
     5701 atacagcttt aaacttaaat gttaaagcaa ttgtagctgc tactgaaagt ggttcaacgg
     5761 cacgtactat ctctaaatat cgtccgcatt cagacattat tgcagtgact ccaagtgaag
     5821 aaactgcacg tcaatgttca attgtttggg gtgttcaacc tgtagttaaa aaaggacgta
     5881 agagtacaga tgcattgtta aacaatgcag ttgcaacagc tgttgaaact ggtagagtaa
     5941 ctaatggtga tttaatcatt attactgctg gtgtaccaac tggtgaaact ggaactacta
     6001 atatgatgaa aatccaccta gttggtgacg aaattgctaa tggtcaaggt attggacgtg
     6061 gatcagttgt tggtactacg ttagttgctg aaactgttaa agatttagaa ggtaaagatt
     6121 tatctgacaa agttatcgtt acaaactcaa tcgatgaaac gtttgtacct tatgtagaaa
     6181 aagcgttagg cttaattaca gaagaaaatg gaattacatc accaagtgca attgttggtt
     6241 tagaaaaagg tattccaaca gttgtaggtg tagaaaaagc tgttaaaaac ataagcaata
     6301 acgtgttagt tacgattgat gctgctcaag gtaaaatctt tgaaggatat gcaaacgtac
     6361 tataatttat aaaaaacgtc tttccattta tcaacaatgg aaaggcgttt tttggttcat
     6421 ctggtatttt atgacgtaat taataggata tttgataatg ataatgtatg aatagcaatc
     6481 tatataaatg tttatatctt ttatacatgc acattatcag ctttaaactt tcactcatat
     6541 tactttggaa agttattata aaatagaagt atgga
//
LOCUS       DAKSQE010000125         6571 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.125, whole genome shotgun sequence.
ACCESSION   DAKSQE010000125 DAKSQE010000000
VERSION     DAKSQE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.125"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..645)
                     /locus_tag="O0V33_002300"
     CDS             complement(<1..645)
                     /locus_tag="O0V33_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HCX8976914.1"
                     /translation="MKKSKAMLNVLLLIINLIAICSVNNAYANEENPKIEDLCKKSSV
                     DDIALHNIDKDYMTNRFTINESTVLTTEKFLDIDLLFKNFTWLDGKSAEFKDLKVEFS
                     SSEISKEYFGKTVDIYGVYYKAHCHGEHQVKTACTYGGVTPHENNKLSEPKEIGVAVY
                     KDNVNVNTFIVTTDKKKVTAQELDIKVRTKLNNVYKLYDRMTSDVQKGYIKFHSH"
     gene            complement(874..962)
                     /locus_tag="O0V33_002301"
     tRNA            complement(874..962)
                     /locus_tag="O0V33_002301"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(925..927),aa:Ser,seq:gga)
     gene            complement(1017..1088)
                     /locus_tag="O0V33_002302"
     tRNA            complement(1017..1088)
                     /locus_tag="O0V33_002302"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1053..1055),aa:Glu,seq:ttc)
     gene            complement(1090..1164)
                     /locus_tag="O0V33_002303"
     tRNA            complement(1090..1164)
                     /locus_tag="O0V33_002303"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1130..1132),aa:Asn,seq:gtt)
     gene            complement(1179..1252)
                     /locus_tag="O0V33_002304"
     tRNA            complement(1179..1252)
                     /locus_tag="O0V33_002304"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1218..1220),aa:Gly,seq:tcc)
     gene            complement(1268..1343)
                     /locus_tag="O0V33_002305"
     tRNA            complement(1268..1343)
                     /locus_tag="O0V33_002305"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1308..1310),aa:His,seq:gtg)
     gene            complement(1357..1429)
                     /locus_tag="O0V33_002306"
     tRNA            complement(1357..1429)
                     /locus_tag="O0V33_002306"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1394..1396),aa:Phe,seq:gaa)
     gene            complement(1446..1521)
                     /locus_tag="O0V33_002307"
     tRNA            complement(1446..1521)
                     /locus_tag="O0V33_002307"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1486..1488),aa:Asp,seq:gtc)
     gene            complement(1543..1616)
                     /locus_tag="O0V33_002308"
     tRNA            complement(1543..1616)
                     /locus_tag="O0V33_002308"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1580..1582),aa:Met,seq:cat)
     gene            complement(2088..2642)
                     /locus_tag="O0V33_002309"
     CDS             complement(2088..2642)
                     /locus_tag="O0V33_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HCX8976915.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAN
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
     gene            complement(2962..4362)
                     /locus_tag="O0V33_002310"
     CDS             complement(2962..4362)
                     /locus_tag="O0V33_002310"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HCX8976916.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPFGKLRAGFDLFKKPTQMQNGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYSNQQEDVF
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTYDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDDELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            complement(4386..5309)
                     /gene="hemH"
                     /locus_tag="O0V33_002311"
     CDS             complement(4386..5309)
                     /gene="hemH"
                     /locus_tag="O0V33_002311"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCX8976917.1"
                     /translation="MTKKMGLLVVAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHKD
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            complement(5367..6404)
                     /gene="hemE"
                     /locus_tag="O0V33_002312"
     CDS             complement(5367..6404)
                     /gene="hemE"
                     /locus_tag="O0V33_002312"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCX8976918.1"
                     /translation="MVHNKNNTILKMIKGEETTHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSGFVHTYTQR"
     gene            complement(6394..6501)
                     /locus_tag="O0V33_002313"
                     /pseudo
     CDS             complement(6394..6501)
                     /locus_tag="O0V33_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2032 a         1324 c          928 g         2287 t
ORIGIN      
        1 atgagaatgg aatttaatat aacctttttg tacatcacta gtcattcgat catataattt
       61 atatacatta tttaattttg ttcttacttt aatatcaagt tcttgtgcag taactttctt
      121 tttatctgta gtaacgataa atgtattaac atttacatta tccttataca cagctactcc
      181 tatttcttta ggttcactta atttattatt ttcatgaggt gttaccccgc cataagtaca
      241 ggcagttttc acttgatgct caccatgaca atgcgcttta taatagacac cataaatatc
      301 tacagttttt ccaaaatact ctttggaaat ttccgatgag ctaaattcca cttttaaatc
      361 tttaaattca gcagattttc catctaacca agtaaaattt ttaaataata aatcaatatc
      421 taaaaatttt tctgtagtta atacagttga ctcatttatt gtaaagcgat tagtcatata
      481 gtctttatca atattatgta gagcaatatc gtctacactt gacttcttac acaaatcctc
      541 aattttagga ttttcttcat ttgcatatgc attgtttaca ctacatattg caattaaatt
      601 tataattaat aataatacat ttaacattgc tttactcttt tttataatat ccctccttca
      661 caaaaagctt tcttatagaa atttttcaat ataaattaaa aaattctggt tttaaccttt
      721 aaatattcac taatactttt tgattacgat tttaaaactt aacaatccta ttaacaattg
      781 aacaaacaca ttttaaacta agtgactcag ataatagaca aaaaacctta aaaatagcgc
      841 ttaaaatggg taaaaccatt ttaagcgcta taacggagag tgagggattc gaaccctcga
      901 gacgcttgtg gcgcctacac actttccagg cgtgctcctt cggccaactc ggacaactct
      961 cctcaacaat aaatatgtaa taaaaaaaca gaagcgatat ttcacttctg tttgtatgac
     1021 tcctacggga ctcgaacccg tgttaccgcc gtgaaagggc ggtgtcttaa ccgcttgacc
     1081 aaggagccat ggctccacag gtaggactcg aacctacgac cgatcggtta acagccgata
     1141 gctctaccac tgagctactg tggaataata gaaaataatg gagcgggtga tgggaatcga
     1201 acccacaaca tcagcttgga aggctgaggt tttgccatta aactacaccc gctaatgtaa
     1261 acgattatgg ggcggctgaa ggggatcgaa ccctcgaatg tcggaaccac aatccgatgt
     1321 gttaaccact tcaccacaac cgccatgaat taagaatggt tcaggacaga gtcgaactgc
     1381 cgacacatgg agcttcaatc cattgctcta ccaactgagc tactgaaccg taatggtaat
     1441 aataatggcg gtctcgacgg gaatcgaacc cgcgatctcc tgcgtgacag gcaggcgtgt
     1501 taaccgctac actacgagac ctattaaatt aaaaactatg tattgcggga ggcggatttg
     1561 aaccaccgac cttcgggtta tgagcccgac gagctaccga actgctccat cccgcgctaa
     1621 tattattttg aattacctaa ttaatatacc ataatcaaaa acctaaagtc aagaactttt
     1681 tgaatttaat ttaaatgtta tctcttaaat aattacttaa atatcgtagc aacatgttct
     1741 ctgttgaaca caaatattag tatattcatt tttgtagtat acgtcaacga catttttaaa
     1801 gttttttgtg taaaaaaacg cttcttattc ccttttatca tataagtgtc taatagttgt
     1861 tataaatagt gttacagcat tttaaaaggt ataggaatta taaagtttac aacgcctata
     1921 ccttctgaaa aagaaattta aaagcttgct acaaccgcat gctcattcag tcaacgacta
     1981 ccaatataac attgtagccc taagacattc cttggcgatt caattaccac atttttttca
     2041 aaatcagcag ctacctacag acacaacata acacaacaat acattaccta tcgtgtcatg
     2101 taatcttgca tccgatcttg caacgctgta aatgtttcga agccatcctc ttctaagaag
     2161 tgccctccat cttccacgat tcgcaagttc ccctctaatg cattcattaa acgctgagtc
     2221 tctttatatg aaacgtattt gtcattttta gaactcaatc cgtaaaaatt gtcaactttc
     2281 tttttaatat tatcgtaatc aatagttaca ttacttaaat cgatatctaa atctatattt
     2341 tctgcatctt ctttaaagcc cgctatactg aaaaagcctt caatcggctg atcaatcatt
     2401 tcaatatatt ttaaagctgt gattgaacct aaaccatgtg ttacaaaata tgtatccttt
     2461 ttgcgtacat taatttgttt cgtcatagct tcaatccact gatccactgt attcgcttca
     2521 ggggattcaa aattaaataa tgttacgtca tatccttcta aagttaagtt atgctccaac
     2581 cactgatacc aatgatttct actatttcca tgcatagaat gtacaatgat tacatctgtc
     2641 atctcattct ctcctttcaa cttactactt cttttctatt taaaaaaatg aatgattacc
     2701 tataattgta aaataaaaac accttaatta gaaatgttat atcgcaaagt gacatttcta
     2761 attaaggtgc attgtcatca tttcaatatc attcaataat agctaaacct tcttcttcga
     2821 ttcagtttca tgaaataatt cccgctgaaa gtatcatgat ttacacatta cttccaccat
     2881 tatataactt aaaaatgact atatttcatc aaacattatc taaaggcgtc gcacctacac
     2941 caactccatc caacaattaa cttacaactc tgcgattact tcttcagcag caactttacc
     3001 ttgtgtaata caatcaggta gtccaaccgc ttcaaaagat gcaccagtta ctctaagtcg
     3061 tggatatgtt tgtttaatat gtgcttgaat ctgcctaatt tgttgaatgt gaccaacatg
     3121 gtactgtggc atacttttcg gcaaacgatt gacaattgta aattcaggat cgcctttaaa
     3181 tgtcatcatt tgacttaaat ctctacgtac aatcgatact aattcatcat ctgtatgatc
     3241 atcaaccaca gtatcacctg gtttacctac atacgcacga atcaaaacct taccttctgg
     3301 tgtagtaaat ggccattttt tcgatgtcca agtacatgcg gtaatgtcag tatcactcgt
     3361 tctcgcaatc acgaagccag taccatcata agtattttca atgtcttttt catcaaatgc
     3421 caatacaaca gttgcaacag tcgtactatc catcgtttta aaataatcaa atgctggatc
     3481 ttgtccgaac caattcaaaa aaacttgatg cggtgttgtc actaataccc catcgaatac
     3541 atcttcttgt tgattactgt aaacaatttt atattgcttt tgagatgtaa taatatcatc
     3601 cactgacgta ttgtaacgta ttgtcacacc tttatttttc acatcttgtt ctaatgcttc
     3661 aataaatgaa cttaaaccat gcttaaattg tttgaattgt ccttttggtg cgccaggata
     3721 taattgtctt tgtttcagac gcttattttt ctcatccttc atacctttta tcagacttcc
     3781 gaatgcctct tctttttctt taaaattagg aaacgtactc atcaaactta atttatcaat
     3841 atcggtacca taaataccac ccattaaagg ctcaattaaa ttctcaagta cctcattacc
     3901 taatcttgct ctgaaaaatg caccaacaga aatgtcacca ttctgcattt gagtaggttt
     3961 tttaaataaa tcaaaccctg ctcttaactt tccaaatggt gaaattaatt tagttgtcac
     4021 aaacggtttg atatctgtcg gaattcccat aattgatcca cctggaatag gatacaattt
     4081 gtttttcgca aaaatataag attgtccagt cgtatttgta acaatatctt gttctaatcc
     4141 aatatctttc gctaattctg tcataatcgt ttttctacct aaataagatt caggccctag
     4201 ttcaatcata taaccatctt tacgatacga ttgaatcttc ccccccggac gattcgatgc
     4261 ttcaaagatg gttacatcaa tattaggatc ttgctgtttt aaaaaatatg cacttgataa
     4321 acctgttatc cccgctccta taatagccac tgatttagtc acaacgtttc acgcttcttt
     4381 cgtcattaaa atatagactt gatttcatca acaattgcac cgataaataa tggatgtgta
     4441 ttcggcattt ttggacgata ataattcgcg ccaatgtcat cgcaaacaac tttacattca
     4501 taatcattgt cataaagcac ctctaaatgc tcacatacaa aacctactgg cgtatatata
     4561 aagtttttat actgatgttt ttcatataaa tcacgtgtta aatcttgtac atctggccct
     4621 aaccaaggtg tacctgtatt accttcagat tgccaaccaa tcgcgatatg ttcaatatta
     4681 gattgttctt taattaaaag cgcagtatgt tctagttctt gtggatatgg atcattattc
     4741 ttttcgatta aaccttttgg caaactatgt gccgaaacaa ctaataccgt gtctttgtgt
     4801 tcctcttccg gtatttgagc taatgtttcg ttgactttat tcgtccaata ttcaataaat
     4861 ttaggttgtt cataataatg tttcacatgt gtaagttgaa taccatattt tgcagcttct
     4921 tcatcagcac gtttgtcata tgatcctact gaaaatgaag aataatgtgg tgctagtact
     4981 acagtgattg cttcagtaat accatctttg tgcatttgtt caaccgcatc ttcgataaat
     5041 ggtgaaatgt gttttaatcc taagtatagt ttaaattcaa catctgcata tgctttattt
     5101 aatgctgaaa ctagcgcatc agcctggtca tctgttgtac ctgctaatgg tgataaacca
     5161 cctataaatt catatctatc tttcaaatct tgaagttctt cttcagatgg acgtttacca
     5221 tgtctaatgt ctgtataata tggctctatg tcactttctt tataaggtgt gccataagcc
     5281 acaactaata atcccatttt tttagtcatt gataatacct tcctttaaat gaattatctt
     5341 tcatgtgctt caatgtaata ctatgattat ctttgtgtat atgtgtgtac gaatccgctt
     5401 actttacgta acgtctctgg ttgcacttct gggaaaacac cgtgtcctaa attaaagatg
     5461 tgtttaccgt tctccatacc ttgatctaat attggtttca atctctcttc aatgacattc
     5521 catggtgcta ataaaattga tggatctaaa ttaccttgta atgttttagt aacgcctaat
     5581 tgttgagcct gattaataga cgttctccaa tctaggccta atacatcaat cggcaaatca
     5641 ttccattcat tgattaaatg actggcacct acaccgaata gaattaccgg cacatcatgt
     5701 ttttctttaa cctcactgat taatcgaatc atatgtggtt taatgtaacg tctgtaatcc
     5761 tcgacattta atgcacctac ccatgaatcg aaaatttgaa tcaattcggc acctgcttcg
     5821 acttgagctg ttacatattt aacagataca tcgactaaat gattcattaa agcaaaccat
     5881 gttgcttcat ctctatacat catcgctttt gtaaaattgt aatttttcga tggtccgcct
     5941 tcaatcatat atgacgctaa tgtaaatggt gccccagtaa atcctattag cggcacattt
     6001 aacttttctt ctgttaaaag tttaattgta tctaatacat atggtacatc tcgttcgggg
     6061 tctatttgag aaagtttctc aacatcttga attgttttga taggattatg aatcactgga
     6121 ccaatacccg atttaatttc tacatcgaca ccaattggct ttaatggtgt cataatatct
     6181 ttgtataaaa ttgctgcatc tgtatgataa ttatcaactg gtaaatgtgt aacataagcg
     6241 cacaactccg gctgatgtgt aatatcgaat agtgaatatt tttctttcaa ttttcgatat
     6301 tctggttgcg aacggccagc ttgtcgcata aaccaaacag gtgtatgtgt cgtttcttca
     6361 cctttaatca ttttcaaaat tgtattgttt ttattatgca ccataaaggc ctcctaaatt
     6421 aaaatcattc ttatctatat tatcatatcg ctcattcgtt cgtattttca ataaataaat
     6481 gtcataaaac tgacattaaa ttacacatca tttgtttgtg tatttaaatt ttaaaatctt
     6541 gatggattat ctccacattt ttttgtcttg c
//
LOCUS       DAKSQE010000126         6538 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.126, whole genome shotgun sequence.
ACCESSION   DAKSQE010000126 DAKSQE010000000
VERSION     DAKSQE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.126"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            151..912
                     /gene="sufC"
                     /locus_tag="O0V33_002314"
     CDS             151..912
                     /gene="sufC"
                     /locus_tag="O0V33_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCX8976919.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RLAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
     gene            1010..2317
                     /gene="sufD"
                     /locus_tag="O0V33_002315"
     CDS             1010..2317
                     /gene="sufD"
                     /locus_tag="O0V33_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCX8976920.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            2423..3673
                     /locus_tag="O0V33_002316"
     CDS             2423..3673
                     /locus_tag="O0V33_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCX8976921.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPVQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDVDQLINALKQTKEFFSYEF"
     gene            3663..4127
                     /locus_tag="O0V33_002317"
     CDS             3663..4127
                     /locus_tag="O0V33_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCX8976922.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            4278..5675
                     /gene="sufB"
                     /locus_tag="O0V33_002318"
     CDS             4278..5675
                     /gene="sufB"
                     /locus_tag="O0V33_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCX8976923.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            5862..6001
                     /locus_tag="O0V33_002319"
                     /pseudo
     CDS             5862..6001
                     /locus_tag="O0V33_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6007..6321)
                     /locus_tag="O0V33_002320"
     CDS             complement(6007..6321)
                     /locus_tag="O0V33_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976924.1"
                     /translation="MYFVLAIFTLISASVSLGYSIQACASSHNINAYYALSRSLPLFL
                     LAIFSLVIHSAIFLITISIAMILVQFLDAIVGYKSKDVFKTYGPLATSVVNLILLIVF
                     LF"
BASE COUNT         2365 a          913 c         1249 g         2011 t
ORIGIN      
        1 aaaaataata aaaagggttt aagttgttta tattaaggta taagtaagtt ataattaact
       61 gaacgcatta ttacaaagtc tttttgacta caaattaaaa ttattataaa ctagttaaga
      121 aaactttata ttttacggag ggaatataaa atggcatcaa cattagaaat caaagaccta
      181 catgtgtcta ttgaggataa agaaatctta aaaggtgtta acttgacaat taacactgat
      241 gaaatacatg cgattatggg accaaacggg acaggtaaat caactttgtc atctgcaatt
      301 atgggacacc caagttatga agtaactaaa ggagaagtac ttttagacgg tgtaaatatt
      361 ttagaattag aagttgatga aagagcaaaa gcaggattat tcttggcaat gcaatatcca
      421 tcagaaatta caggtgttac aaatgctgat ttcatgcgtt tagctatcaa tgcgaaacgt
      481 gaagaaggac aagaaatcaa cttaatgcaa tttattaaga aattagataa aaatatggat
      541 tttctagaca tagataaaga catggcacaa cgttatttaa atgaaggttt ctcaggtgga
      601 gagaagaaac gtaacgaaat cttacaatta atgatgttag aacctaagtt tgcaatctta
      661 gatgaaatcg attcagggtt agacatcgat gcattaaaag ttgtatctaa aggtattaac
      721 caaatgcgtg gggaaaactt tggtgcatta atgatcacac actatcaacg attattaaat
      781 tacattactc ctgataaagt acatgtaatg tatgctggta aggtcgttaa atctggtggt
      841 ccagaattag caaaacgtct tgaagaagaa ggatatgaat gggttaaaga agagttcggt
      901 tcagctgaat aatcttatta atacagtatc catgagatgt tcatctatat atgatgaaaa
      961 tgaacattta tacgaaatag taaatttcat caagtaggag gaaaaagtta tgacaactga
     1021 tattttgaac atttctgaag aacaacttgt tgattattct aaagcccaca atgaaccttc
     1081 ttggatgaca gaattacgta aaaaagcttt gaaattaaca gaaactttag aaatgccaaa
     1141 acctgataaa acaaaattaa gaaaatggga ttttgattct tttaaacaac acgatgtaaa
     1201 aggtgatgtt tatcaatctt tatcacaatt acctgagtca gtaagagaaa ttattgacgt
     1261 agatcattct aaaaacttag taattcaaca taataatacg attgcgtaca cacaagttga
     1321 tgataatgca tcgaaagatg gcgttatcgt tgaaggttta gcagatgctc ttatgaacca
     1381 tagtgattta gtacaaaagt actttatgaa agatgcagta acagtagatg aacatcgtat
     1441 cacagcgcta cacacggcat tagttaatgg tggcgtattt gtttatgttc ctaaaaatgt
     1501 agttgtagaa catccagtac aatacgttgt gttgcacgac gacgaaaatg caagctttta
     1561 taaccatgtc atcatcgtta ctgaagaaag cgccgaagtc acatatgttg aaaattactt
     1621 atcaaatgca tctggtgaag gaaatcaatt aaatattatt tctgaagtga ttgctggtgc
     1681 aaattcaaat atcacatatg gctcagtaga ctatatggat aaaggcttta caggtcatat
     1741 cattcgacgt ggtattactg aagcggatgc ctcaattaat tgggcattag gtttaatgaa
     1801 tgagggtagc caaattattg ataatacaac aaatttattt ggtgatcgtt caacaagttc
     1861 acttaaatca gtagttgtag gtacaggcga acaaaaaatt aatctaacat ctaaaatcgt
     1921 acaatatggt aaagaaactg atggttatat ccttaaacat ggtgttatga aagaacatgc
     1981 atcatctgta tttaatggta tcggctacat taagcatggt ggaactaaat caattgctaa
     2041 tcaggaatca cgtgtattaa tgttatctga acatgctcgt ggtgacgcga atcctatttt
     2101 attaattgat gaagatgatg tacaagctgg tcatgctgca tcagtaggtc gtgttgatcc
     2161 agatcaactt tactatttaa tgagtcgtgg gatttctcaa agagaagcgg aacgtcttgt
     2221 tatacatggt ttcttagatc cagtagtacg tgaattacct atcgaagacg ttaaacgtca
     2281 attgagagaa gtaattgaac gcaaagtttc taaataatat tttgaaaata aaagtttgta
     2341 atagatatag actgtcgata ttggtataag actaatacaa cgtcagtatt taaatgatta
     2401 ggatttttat ttaagaaagg tcgtgaatga agtggccgaa cactcatttg acgttaatga
     2461 agtaatcaag gattttccga tattagatca aaaagtcaat ggcaaacgtt tagcatatct
     2521 tgattcaaca gcgacaagtc aaacgcctgt gcaagtgtta aatgttttag aagattacta
     2581 caagcgttat aattcaaacg ttcatcgtgg tgttcataca ttaggatcat tagcaactga
     2641 tggttatgaa aatgcccgtg aaaccgttcg tcgttttatt aatgcgaagt attttgaaga
     2701 aatcattttt acacgcggaa caactgcgtc gattaacctt gtagcacata gctatggtga
     2761 tgcaaatgtt gaagagggcg atgaaattgt tgtcactgaa atggaacatc atgccaatat
     2821 tgttccttgg caacagttag caaagcgtaa aaatgcgaca ttgaaattta taccaatgac
     2881 agctgacggt gaattaaaca tcgaggatat taagcaaacg attaatgata aaacaaagat
     2941 cgttgctatt gcacatattt ctaatgtact cggtacaatt aatgatgtta aaaccattgc
     3001 agaaatagct catcaacatg gtgcaattat cagtgttgat ggggcgcaag cagcaccaca
     3061 tatgaaactt gatatgcaag aaatgaatgc tgatttttat agttttagtg gacataaaat
     3121 gcttggacca acaggtattg gcgtattatt tggtaaacgt gagttactac aaaaaatgga
     3181 accgattgag ttcggtggcg acatgattga ttttgtaagt aagtatgatg caacatgggc
     3241 tgatttacct actaaatttg aggcgggtac tccattaatt gctcaagcaa ttgggcttgc
     3301 agaagctatt cgctatttag aacgcatagg ttttgatgca attcataaat atgaacaaga
     3361 attaacgata tatgcttatg agcaaatgtc tgcaattgaa ggaattgaaa tttatggccc
     3421 gccaaaggat cgtcgtgcag gtgtaataac gtttaattta caagatgtac atccacacga
     3481 tgttgctaca gccgtagata cagaaggtgt agcggttaga gctgggcatc attgtgcgca
     3541 accgttaatg aaatggttaa atgtgtcttc aacagctaga gcgagttttt atatatacaa
     3601 cacgaaagaa gacgttgatc agttaattaa tgccttgaaa caaacgaagg agtttttctc
     3661 ttatgaattt taataatcta gatcaattat atagatctgt cattatggat cattataaaa
     3721 atcctagaaa taaaggtgta ttagataacg ggtctatgac agtagatatg aataacccga
     3781 catgcggtga ccgtatacga ctaacatttg atatagaaga cggcattata aaagatgcta
     3841 agtttgaagg tgaaggttgt tcgatttcaa tggcaagtgc atcgatgatg acacaagccg
     3901 ttaaaggtca ttcacttgga gaagcaatgc aaatgagcca agaatttacg aaaatgatgc
     3961 ttggtgaaga ctatgtgatt acagaagaaa tgggagatat tgaagcattg caaggtgtat
     4021 ctcaattccc agctcgtatt aaatgtgcca cattagcttg gaaagcattg gaaaaaggta
     4081 ctgttgctaa agaaggtaaa gcagaaggta cgactgaaga agaatagaat gctgttaatc
     4141 atagatgatt ttgatattag acatataata gtaaaaaaat ttttatatga tgtcatgtca
     4201 ttgttataat atggtttaca tcatgaatta aaaacttacg cacgccgttg taaatatatt
     4261 tttaaggagt gattgaaatg gctaaaaaag cacctgatgt tggggattat aaatatggat
     4321 tccacgacga tgatgtgtcc attttcagat cagaacgtgg tttaactgag aatatcgtta
     4381 gagaaatttc taacatgaaa aatgagccgg aatggatgtt agatttccgt cttaaatcat
     4441 taaaattgtt ttataaaatg ccaatgcctc aatggggtgg cgacttatca gaattgaatt
     4501 tcgatgacat tacttactat gtaaagcctt cagaacaagc tgaacgttca tgggatgaag
     4561 tgcctgaaga aattaaaaga actttcgata aattaggaat tcctgaagct gaacaaaaat
     4621 atttagctgg tgtttctgct caatatgaat ctgaagttgt ttaccataat atggaaaaag
     4681 aacttgaaga aaaaggtatt atctttaaag atacagatag tgctttacaa gaaaatgaag
     4741 aattattcaa aaaatacttt gcttctgtag tacctgcagc agataacaaa tttgcggcgt
     4801 taaactcagc agtatggtca ggtggttcgt tcatttatgt acctaaaaat atcaaactag
     4861 atacgccact acaagcttat ttccgtatta actctgagaa catgggtcaa tttgaacgta
     4921 cattaatcat tgctgatgaa ggtgcttctg tacattacgt agaaggttgt actgcaccag
     4981 tttatacaac tagttcttta cactctgctg ttgtggaaat cattgtgcat aaagatgcgc
     5041 acgttcgtta tactacgatt caaaactggg cgaacaatgt atacaattta gttacgaaac
     5101 gtacttttgt ttatgaaaac ggaaatatgg aatgggtaga tggtaactta ggttctaagt
     5161 taacgatgaa atatccaaac tgtgttcttt taggtgaagg tgcaaaaggt agtacattat
     5221 ctattgcatt tgctggtaaa ggacaagttc aagatgccgg tgctaaaatg attcataaag
     5281 cgccaaatac atcttcaacg attgtttcta aatctatttc taaaaatggt ggtaaagtta
     5341 tttatcgcgg tattgttcat tttggacgta aagcaaaagg cgctcgttca aatattgaat
     5401 gtgatacatt aatcttagat aacgaatcaa catcagatac aattccatat aacgaagtat
     5461 tcaacgatca aatatcatta gaacatgaag cgaaggtttc aaaagtttct gaagaacaat
     5521 tattctatct aatgagtcgt ggtatttctg aagaagaagc gacagaaatg attgttatgg
     5581 gattcatcga accatttaca aaagaacttc caatggaata cgcggtcgaa atgaaccgtt
     5641 taatcaagtt cgaaatggaa ggtagtattg ggtagcttta aaccgcgttg ttaagccatt
     5701 cttaactgtc gaaaacggtt attgatacca ttttgatact gaatataaca aaaagccaca
     5761 ttactgtggc tttttttgtt ttataactaa atcggattga tagataagct ttgtacttat
     5821 ttatatcagt ccgatttttt gattggtgta aaaaataatc attgatgcta gataaagcga
     5881 caacataaat acaacatgat tgtggcatta gagtgctggt ctttattaaa ttaattgaaa
     5941 actacatcaa atatccttta aagataattc gataatagtt cgattaagtt tcgttgtata
     6001 agtgagttaa aataagaaaa ctattaataa aattaagttc actacagatg ttgctaatgg
     6061 accgtaagtt ttaaagacat ctttactttt ataaccaaca atcgcatcta aaaattgaac
     6121 taaaatcatt gcaatggata tagttatcaa aaatatagca ctatgaatga ctaaagaaaa
     6181 aatagctaat aaaaataaag gtaagcttcg actaagtgca taatatgcat ttatattatg
     6241 gctagatgca caagcttgaa ttgaataacc taaactcaca ctggcactaa tgagtgtaaa
     6301 tattgctaaa acaaaataca tgttaatcct tctttctata tttgaatatc tgtaaacaag
     6361 tacttgtcta aagatattta aaagataatt agaataaatt tatgagaaac tttttgttat
     6421 cattatagcg atttcaaatc attataacta tgtcataaac taaatttgtt aaaatttttc
     6481 attatgtaaa tttattaata acaattaggt cgaattatag catcatttta ctaataaa
//
LOCUS       DAKSQE010000127         6513 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.127, whole genome shotgun sequence.
ACCESSION   DAKSQE010000127 DAKSQE010000000
VERSION     DAKSQE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6513
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.127"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(205..711)
                     /locus_tag="O0V33_002321"
     CDS             complement(205..711)
                     /locus_tag="O0V33_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCX8976925.1"
                     /translation="MAHIIRRVSIKDVENFISMLAKIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            824..1747
                     /gene="graX"
                     /locus_tag="O0V33_002322"
                     /gene_synonym="apsX"
     CDS             824..1747
                     /gene="graX"
                     /locus_tag="O0V33_002322"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HCX8976926.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFAISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTTVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIVETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESSVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            1763..2437
                     /gene="graR"
                     /locus_tag="O0V33_002323"
                     /gene_synonym="apsR"
     CDS             1763..2437
                     /gene="graR"
                     /locus_tag="O0V33_002323"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HCX8976927.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVIDLSKDSIQKGDQTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEIGMDSAIETKVGK
                     GYMAHE"
     gene            2430..3470
                     /gene="graS"
                     /locus_tag="O0V33_002324"
                     /gene_synonym="apsS"
     CDS             2430..3470
                     /gene="graS"
                     /locus_tag="O0V33_002324"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HCX8976928.1"
                     /translation="MNNLKWVVYFLKSRKNWIFWILFLNILMLGISLIDYDFPIDSLF
                     YIVSLNLSLTLIFLILTFFKEVKLYRHFEKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ILAHKDKVVDQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEENQERKQALLFEWSR
                     INSMLDTQLYITRLESQRKDMFFDYVSLKRMVIDEIQLTRHISQVKGIGFDIDFKVDN
                     HVYTDIKWCRMIIRQILSNALKYSENYNVDISTELIDQHVALIIKDHGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVDSVKDQLGIQLQVTSTIGKGTTVKLIFPLQNEI
                     VERMSEVTNLSF"
     gene            3617..4378
                     /gene="vraF"
                     /locus_tag="O0V33_002325"
     CDS             3617..4378
                     /gene="vraF"
                     /locus_tag="O0V33_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HCX8976929.1"
                     /translation="MTILEVKQLTKIYGNKKMAQEVLRDINMSVEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGHKLEKLSNKALSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKDTMLDRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLMKMNEEIKTTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGINYEF"
     gene            4368..6257
                     /locus_tag="O0V33_002326"
     CDS             4368..6257
                     /locus_tag="O0V33_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8976930.1"
                     /translation="MSFNEIIFKNFRQNLSHYAIYLFSLITSVVLFFSFVALKYTHSI
                     NITETYPIIKEGSQVGSYFLFFIIVAFLLYANVLFLKRRSSELALYQTLGLSKFNIIY
                     ILMFEQLLIFSITAILGITIGIFGSKILLMIVFTLLGIKEKVPIIFSFRAVFETLLLI
                     GVAYFLTAAQNLILVLKQSISELSKHNQAKQMNHNKITFEEVILGILGIILIISGYYY
                     SLRIVHYYDAIGVLMFILFATVIGAYLFFKSSVSLVFKIIKSFKKGVISVNDVMFSSS
                     IMYRIQKNAFSLTVMAIISAITLSVLCFAAISRATLLSEIKYTSPHDVTIKDQQKANQ
                     LASELNNRKIPHFYNYKEVIHTKLYKDDLFDIKAKEPYNVTITSDKYIPNTDLKRGQA
                     DLFVAEGAIKDLVKHKKHGKAVIGTKKHHVDIKLRKDINKIYFMTDVDLGGPTFVLND
                     QDYQEIRKYTKPKHIVSQFGFDLKHKKEMLALEKATNKVDKSIETRSEAISSISSLTG
                     ILLFVTSFLGISFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMAKGLKFKIA
                     FNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPIFIVMGLYICMYAIFAMTAYNHSKRT
                     IRHSI"
BASE COUNT         2374 a          842 c         1140 g         2157 t
ORIGIN      
        1 aaactaaata atataaagca ccactaatcg tagttgatca attacacatt agtggtgttt
       61 tattttgaat ttaaatttga taaatgaata agagtatacg cattcattta agattagatg
      121 acattgataa gtatgagtaa tgtattaggt aaccattgaa ttcgttgtta tacgattcta
      181 atacaagatt atgataaaat agctttaatt aagaatttta gccatcatat agtcatcgta
      241 atatttacca tcgatgaata acttatcttt taaaacgcct tcgatttgaa aatcggcact
      301 tttaaaaagc tctagggcag gttggttatt gagtggtaca tttgcttcaa ttcggtgtat
      361 ttgattgttt aaacaccaag ccataatggc atcaagaagt gcttggccaa ttccacgatg
      421 ttgatataat ttctttacac ctaaatcaat tttagcaaca tgtttaatgc gttgaaatgg
      481 tgtcgtatta acaaaggcaa agcctacaag ttgttcatca ctttcagcaa caaagatgac
      541 tttatgtgga gaagtgatat attcttctaa ttgtttacta gccgatgtga cgctaggatc
      601 atattctcct ggtgtgtaga acatatacgg agattcgtcg tatattttcg ctaacattga
      661 aatgaaattt tctacatctt tgatactaac tctacgtata atatgggcca taaaaagcct
      721 ccagtatttt gaaattttaa aacatttgct actattataa tatatattta accaaaaggt
      781 agagtaatat gttttttggt tacagtttaa aggagtattt tagatgaaac ctaaagtttt
      841 attagcaggt ggaacagggt atattggtaa gtatttaagt gaagtgattg aaaatgatgc
      901 tgaacttttt gctatatcaa aatatccaga caataaaaaa acagatgatg ttgaaatgac
      961 ttggattcag tgtgatatat ttcattacga acaggttgtt gccgcaatga atcaaataga
     1021 tattgctgta ttctttatcg atccaacaaa gaattctgcc aaaataacac aatcatcagc
     1081 aagagattta acattaatcg cagcagataa ttttggtcga gcagcggcta tcaatcaagt
     1141 aaaaaaagta atctacatac ctgggagtcg ttatgataat gaaacaattg aacgcctagg
     1201 tgcatatggt acaactgtag aaacaacaaa tttagttttt aaacgttctt tagttaatgt
     1261 agaattacaa gtttcaaagt atgatgatgt tagatcaacg atgaaggtag ttttaccaaa
     1321 gggatggaca ttaaagaacg ttgtaaacca ttttattgca tggatgggtt acactaaagg
     1381 aacttttgtg aaaacagaaa aatcacatga tcaatttaag atatatatta agaataaggt
     1441 gcgaccactc gcagtattta aaatagtaga aacagctgat ggaataataa ctttaatttt
     1501 attgagtgga agtttagtga aaaaatatac agttaatcaa gggaagttag aatttagatt
     1561 aatcaaagaa tcatcagtcg tttatataca tctatacgat tatatccctc gattattttg
     1621 gccgatttat tactttatac aagcaccaat gcaaaaaatg atgattcatg gctttgaagt
     1681 tgactgccgg attaaagatt ttcaaagtcg attaaaatca ggagaaaata tgaaatatac
     1741 taaatgatat tgggtgatat ggatgcaaat actactagta gaagatgaca atactttgtt
     1801 tcaagaattg aaaaaagaat tagaacaatg ggattttaat gttgctggta ttgaagattt
     1861 cggcaaagta atggatacat ttgaaagttt taatcctgaa attgttatat tggatgttca
     1921 attacctaaa tatgatgggt tttattggtg cagaaaaatg agagaagttt ccaacgtacc
     1981 aatattattt ttatcatctc gtgataatcc aatggatcaa gtgatgagta tggaacttgg
     2041 cgcagatgat tatatgcaaa agccttttta tacaaatgta ttaattgcta aattacaagc
     2101 gatttatcga cgagtgtatg aatttactgc tgaagaaaaa cgcacgttaa catggcaaga
     2161 tgctgtcatt gatctatcaa aagatagtat acaaaaaggt gatcagacga tttttctatc
     2221 caaaacagaa atgattattt tagaaattct tattactaaa aaaaatcaaa tcgtttctag
     2281 ggatacaatt attactgcgc tatgggatga tgaagcattt gtcagtgata acacattaac
     2341 agttaatgtg aaccgattac gtaagaaact atctgaaata gggatggaca gcgcaattga
     2401 aacaaaagta ggaaaaggat atatggcaca tgaataattt gaaatgggta gtttattttc
     2461 tgaaatcaag aaagaattgg atattttgga tactgttttt aaatattctt atgttaggca
     2521 ttagtcttat tgactatgac tttccaatcg atagtttgtt ttatattgtt tctttaaatt
     2581 taagcttaac actgattttt cttatattga ctttttttaa agaagtaaaa ttatatagac
     2641 attttgaaaa agataaagaa atcgaagaaa taaaacataa agatttagcg gaaaccccat
     2701 tccaacgtca tacagttgat tatttatatc gtcaaatttt agcgcataaa gataaagtgg
     2761 tcgaccaaca gttgcaatta aacatgcatg aacaaaccat cacagaattt gtgcacgaca
     2821 tcaaaacacc agtgactgct atgaaattgt taattgacca agaagagaat caagaaagaa
     2881 aacaagcgct attatttgaa tggtcacgta ttaactcgat gttagatacg caattgtata
     2941 ttacgagatt agagtcgcaa cgtaaagata tgttttttga ttatgtctca ttgaagcgca
     3001 tggttattga tgaaattcaa ttaacaaggc atataagtca agttaaaggt atcggatttg
     3061 atattgattt taaagtggat aatcatgtgt acacggatat aaaatggtgt cgtatgatta
     3121 ttagacagat tttatcaaat gctttgaaat atagtgagaa ttataatgtt gatatttcaa
     3181 cggaattaat tgatcaacat gttgcgttaa tcatcaagga tcatggaaga ggtattagta
     3241 aaaaagatat gcctcgaata tttgaacgtg ggtttacgtc aacggctaac agaaatgaaa
     3301 cgacatcatc cggcatgggt ctatacttag tggatagtgt gaaagatcaa ttaggaatac
     3361 aattacaagt tacttcgact attggaaaag gaacaacggt taaattgatc ttcccattac
     3421 aaaatgaaat tgttgagcgc atgtcagaag tgacaaatct gtcattttaa acatgcgttt
     3481 tgttacttag aattgataca tcatgaccac ttcaacgata taatcaaata gatgttaatt
     3541 aaatacaaaa ttgaaggtaa gcgtgccaaa ttgaatataa aggaaatgaa gttaagataa
     3601 atataggagt gttaaagtga caatattaga agtaaaacaa ttaacaaaaa tatatggcaa
     3661 caagaaaatg gcacaagaag ttctgcgaga tatcaatatg tctgttgaag aaggtgaatt
     3721 tattgccatt atgggacctt caggatctgg taaaacgacc ttattaaatg ttttgagttc
     3781 aattgattat atttcgcaag gttctatcac actaaaagga cacaaacttg aaaagctttc
     3841 aaacaaagca ttatctgata ttcgaaagca tgatattggt tttatttttc aagaatataa
     3901 tttacttcat acattaactg ttaaagaaaa tatcatgtta ccattaacgg ttcagaaatt
     3961 agataaggac acaatgttag atcgttatga aaaagtggca gaagcattaa atatattaga
     4021 catcagtgat aaatatccat ccgaattgtc tggtggtcaa agacaacgaa catcagctgc
     4081 cagagcattt ataacattgc cttctattat atttgccgat gaacctacag gtgcactcga
     4141 ttcgaaaagt acacaagatt tattaaagcg attaatgaaa atgaatgaag agattaagac
     4201 gacaattatt atggtgacac atgatccggt tgcagctagc tatgcaaatc gagtagtgat
     4261 gcttaaagat gggcaaatat tcacggagtt atatcaaggt gatgatgaca aacatacttt
     4321 ctttaaagaa atcatacgtg tacaaagtgt tttaggtggc attaattatg agttttaatg
     4381 agattatatt taaaaatttc cgccaaaatt tatcacatta tgccatttat cttttttcgc
     4441 taattacaag tgtagtttta ttttttagct tcgtagcact aaaatatacg catagcatta
     4501 acataactga gacatatccc attattaaag aaggatcgca agtaggtagt tactttctat
     4561 tttttatcat tgttgccttt ttgctatatg ccaatgtact atttcttaaa cgtcgtagtt
     4621 ctgaacttgc attatatcaa acattaggtt tatccaaatt caacattata tatatattga
     4681 tgtttgaaca attattaata ttttcaataa cagctatatt gggcattacg attggtatct
     4741 ttggttctaa aattttatta atgatagtgt ttactttatt aggtatcaaa gaaaaggttc
     4801 caattatttt cagctttaga gcagtgtttg agacactatt attaattggc gttgcttatt
     4861 tcctaactgc agctcaaaat cttatcttag tattgaaaca atctatttca gaactatcaa
     4921 agcataatca ggctaagcaa atgaatcata ataaaattac atttgaagaa gttatactag
     4981 gaattctagg tattatttta atcatcagtg ggtattacta ctctttacgc attgtacatt
     5041 actatgatgc aattggcgtc cttatgttta tattgtttgc gacagtaatt ggggcgtatt
     5101 tattttttaa aagttcagta tccttggtat ttaaaattat taaaagcttt aaaaaaggtg
     5161 taataagtgt aaatgatgtc atgttttctt catctattat gtatcgtatt cagaaaaatg
     5221 cattttcact tactgttatg gcaattattt cagctattac tttatcagta ctttgttttg
     5281 ctgctatcag tagagcaact ttattaagtg aaattaagta tacgtcacct catgatgtta
     5341 cgattaaaga tcaacagaaa gcgaatcagt tagcaagtga attgaacaac cgaaaaattc
     5401 ctcattttta taattataaa gaagtgattc atacaaaact ttataaggat gacttatttg
     5461 atataaaagc aaaggaacca tataacgtaa caattactag tgacaaatat attcctaata
     5521 cggatttgaa acgtggtcag gctgatttat ttgtagcgga aggcgctatt aaagatttag
     5581 tgaagcataa gaaacatggt aaggcagtaa taggaacgaa aaagcaccat gtggatatta
     5641 aattacgtaa agatatcaat aaaatttact ttatgactga tgttgactta ggtggaccga
     5701 cgtttgtgtt aaacgatcaa gattatcaag agataagaaa gtatactaag ccaaaacaca
     5761 ttgtttctca atttggattt gatttaaaac ataaaaaaga aatgttagca ttggaaaaag
     5821 caacaaataa agttgacaaa tctattgaaa caagaagtga agccataagt tcgatatcga
     5881 gtttaacggg aatattgtta tttgtaacct catttttagg aattagtttc ctaattgccg
     5941 tatgttgtat catatacatt aaacaaatag atgaaactga agacgagtta gaaaattata
     6001 gtattcttag aaaacttgga tttacacaaa aagatatggc aaaaggatta aaatttaaaa
     6061 ttgcgtttaa ttttgggtta ccattagtca ttgcactttc acatgcatac tttacatcat
     6121 tagcatatat gaaattgatg gggacaacga atcaaatacc gatatttata gtaatgggac
     6181 tatatatttg tatgtatgcc atttttgcaa tgacagctta taatcattct aagcgtacaa
     6241 tcagacattc aatttaatat atatatagcg gctttcagta ggctagtgga ttaagattca
     6301 tgagctttac tgaaagcttt tatttttgga tagttatagg tccgggcacg acagttttat
     6361 tgtatatgct tcaactttgt aaattgtatt aaatgtgaga aaatagaggc ataggcattg
     6421 aaagaaatat gaattaagaa tagttagaag aaagataagt gtattttaat atgtaaaagt
     6481 tatgtaataa atattgtatc gttgcaaatt ttc
//
LOCUS       DAKSQE010000128         6493 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.128, whole genome shotgun sequence.
ACCESSION   DAKSQE010000128 DAKSQE010000000
VERSION     DAKSQE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6493
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.128"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            110..1306
                     /locus_tag="O0V33_002327"
     CDS             110..1306
                     /locus_tag="O0V33_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCX8976931.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            1296..4232
                     /locus_tag="O0V33_002328"
     CDS             1296..4232
                     /locus_tag="O0V33_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCX8976932.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQANIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTVFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVYYSEAITDEIEVIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            4229..5170
                     /gene="yhaM"
                     /locus_tag="O0V33_002329"
     CDS             4229..5170
                     /gene="yhaM"
                     /locus_tag="O0V33_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HCX8976933.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(5291..6253)
                     /locus_tag="O0V33_002330"
     CDS             complement(5291..6253)
                     /locus_tag="O0V33_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HCX8976934.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFKKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSDVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
BASE COUNT         2377 a          919 c         1099 g         2098 t
ORIGIN      
        1 gctaatgaat cactacacct ataagtatga atatagtgat tagaatgctt tgtatagttg
       61 gattttgcaa aattgatgtt agaaccttta attaaggaga tatggatgta tggttaaatt
      121 tattcattgt tcggatttac atttagatag tcctttcaaa tctaagagtc atataagccc
      181 taaaattttt gaggatgttc aaaaaagtgc ttatgaaagc tttaaaaaca ttgtagatat
      241 tgcattacaa caagatgttg attttgtaat tatcgcgggt gatttgtttg atagtgaaaa
      301 cagaacatta cgtgctgaaa ttttcttaaa acagcaattt gaacgtttac aaaatgaaca
      361 aatctttgtt tatgtttgtc atggtaatca cgacccatta tcatcaaaga tttcatcaaa
      421 ctggccagat aatgtttctg tattttcaaa taaagttgag acgtatgaag caattactaa
      481 atctggtgaa acaatttata ttcacggatt tagttatgaa aatagagcaa gttatgagaa
      541 caagattgat gaatatccat caagtcaagg ccaaaaaggc atacatattg gtgtcttgca
      601 tggtacgtac agtaaatctt cagttaacga aagatatacc gagttcattt tagaagattt
      661 aaacagtaaa ttgtatcatt attgggcttt aggtcatata catgaacgtc aacaattaag
      721 tgatatgcct gtaattaact attcaggtaa tattcaaggt agacatttta atgagcaagg
      781 tgaaaaaggt tgcttattaa tcgagggtga ccacttaaaa ttaaaaacta aattttatcc
      841 tacacagtat attagatttg aagaagcaac tattgaaacg gataagacat ctaagcaagg
      901 tttatacgag gtcattcaaa actttaaaga acaagtgaga gaagaaggga aagcctttta
      961 tcgtttaacg cttgttatta atagtgagac attaatttca cctcaagatt tattacaagt
     1021 tgaagaaatg attacagatt atgaagaaaa cgaaaatcaa tttgtatata ttgatgagtt
     1081 aaaaatacaa tatgcacaaa atgatgagtc acctttagtt aatgaatttt cagcggaatt
     1141 attagtcgat caaactgttt ttgataaagc gatgtcagac ttatatttaa atccaagggc
     1201 atctaagttc ctagacgatt atggaacatt cgaccataca gcattagtta atcgtgctga
     1261 agaaatatta aaagctgaaa tgagaggtga acaaaatgat aattaaatca cttgaaattt
     1321 atggttacgg tcaatttgtt caacgtaaaa ttgaatttaa taaaaacttc actgaaattt
     1381 ttggtgaaaa tgaagcgggt aaatcgacga ttcaagcatt catccattcg atattatttg
     1441 gttttccaac taaaaagtct aaagagccaa gactagaacc acgtctaggt aaccaatacg
     1501 gtggtaaatt agtacttatt cttgatgatg gcttagagat tgaagttgaa cgaattaaag
     1561 gcagtgctca aggtgatgtg aaagtatatt tacctaatgg tgctgtgcgt gatgatgctt
     1621 ggttacaaaa gaaacttaat tatatttcta aaaagacata tcaaggtatc ttttcatttg
     1681 atgtactagg gcttcaagac attcatagaa atctaaatga aaaacaattg caagattatt
     1741 tattacaagc aggggcttta ggatcaactg aattcacgtc aatgcgcgaa gtgattaatc
     1801 gtaaaaaaga tgaattatat aaaaaatcag gtaaaaatcc gattattaat caacaaattg
     1861 agcaattaaa acaactagaa agtcaaattc gtgaagaaga agctaaacta gaaacatatc
     1921 atcgtttagt agatgatcga gataaatcat cacgtcgatt agagaattta aagcataatt
     1981 taaatcaatt atcaaaaatg catgaagaaa aacaaaaaga ggttgcttta catgatcatt
     2041 cacaagaatg gaagtctcta gaacaacagt taaatattga gccaatcaca ttcccagaaa
     2101 aaggtgtaga tcgttacgaa aaagcacgag cgcataagca atcgttagaa agagatattg
     2161 gtttaagaaa tgagcgttta gctcaactta aagaagaagc gactcaatta gagccagtta
     2221 aacaatctga tattgacgcc ttcattagtt tgaatcaaca agaaaatgaa attaaaaata
     2281 aagaatttga acttactgca atcgaaaagg atattgcgaa taaacaacgt gataaagatg
     2341 aattgcaagc aaatattggt tggtctgaaa cgcatcatga cgtagatagc tctgaggcaa
     2401 tgaaaagtta tgtcagtgag caaatcaaga ataaacaaga acaagcggca tacattaagc
     2461 aattagaacg tagtttagaa gaaaataaaa tcgaagataa tgcggttcat agcgaactag
     2521 attctgttga agaaaaaata gttcctgaag agacttttga aaagaaaaaa gaatactcac
     2581 aacaagtcat tgaattaaac gaaaaagaga acttgtatag caagttgaaa gaacgtttcg
     2641 aaattgagca acaagaaaaa caaaaacgtc aaaaattgtt acgaacaacg tttattcttt
     2701 taacattagt aggtattggt ttaactgtct tctcatttat ttcaaacaac atgttgtttg
     2761 gtattatttt cgcagtatta actttagtat ttgttatcgg tatcatcatg tctaaatcaa
     2821 aagaagtata ttatagcgag gccatcacag atgaaatcga agtaattaaa gcacaactag
     2881 ccattttaga tgaaaactat gacttagatt ttgatcttga tgagcaatat cgcattcgtg
     2941 atcactggca acaagctttg aaaaataaag atattttaga agagaaacgt cagtatattg
     3001 aaggtcgttt aaatgatgcg aaagggcgtc atgatgaatt acaatcgaca gttgaaaacg
     3061 ttaaggatga attatactta tcatctaaaa tttcaaatga tttaattgtt gatagtattt
     3121 ctacaatggc gaatattaaa gcattagatc aacatatcag tgatttaaat caacaacgcc
     3181 agcaattggt tcaagaatta gatacattct acaatcatgc tgaagctgta acaaaatcac
     3241 aatttgttta tttcaacaaa ttatctttat tccacgatgt gcaacaatgg ttgaaatcag
     3301 ctgaagacac gaatgaaaaa tggcgtatta atgctgaaaa taccaaactt gttacaaatg
     3361 aattaaatca tttaaatgct caattagaag aaaataataa agaaattaca gcactattcg
     3421 attttatcaa tgttggtact gaagaagatt tttaccaaca tcatgaagat taccaaacat
     3481 acactagtaa tttgagtcgt tttaatgatt taacaaaata tcttgagaac caaaactatt
     3541 cttatgaatt aagttctagt ttaagtgaaa aaacaacagc acaacttgaa gaagaagatc
     3601 atttattggc tactcaagtt gacgaatata atgagcaata tcttgaaatg caagcacaag
     3661 tcagtgattt aagtgcacaa atcaatcaca tggaaactga tacaacgctt gctaatttaa
     3721 gacatgaata tcacagtctt aaaaatcaac ttaatgatat cgcaaaagat tgggcaagtt
     3781 taagttattt acaaagttta gttgatgaac acattaaaca aattaaagat aaacgtttgc
     3841 ctcaagttat taatgaagcg gtagaaatat tgaagcattt aacagatggc agatatacga
     3901 tgattaacta taatgaagat tcaattacgg ttaaacatgt taatggtcaa ttatatgatc
     3961 ctgttgaact aagtcaatct acaaaagaat tactttatgt agctttacgt atcagtttaa
     4021 ttaaggtact acgaccatat tatccgttcc cattaattgt tgatgatgca tttgttcatt
     4081 ttgataaaaa acgtactgaa aaaatgttga attatttacg atcattatca gaacactatc
     4141 aagtactata ctttacatgt gtaaaagata atattgttcc atcaaaagaa gtgattacat
     4201 taaacaaaat agaggaaggc gggaaacgat gagaaatata gagaatctaa atcccggaga
     4261 ttcagttgat cactttttct tagtgcataa agctacacag ggtgtaacag cacaaggtaa
     4321 agattatatg acattacatt tgcaagataa aagtggtgaa attgaagcga aattttggac
     4381 ggctacaaaa aatgatatgg caacaatcaa gcctgaagaa attgtacatg ttaaaggtga
     4441 catcataaac tatcgcggaa ataaacagat gaaagtcaac caaattagac tagcgacaac
     4501 tgaagatcaa ttaaaaacag aacaatttgt agatggtgca cctttatcac cggcagaaat
     4561 acaagaagag atttctcatt atttgctaga tattgaaaat gctaatttac aacgtatcac
     4621 acgtcattta ttgaaaaaat atcaagaacg attttacaca tatccagctg ctagttctca
     4681 tcatcataac tttgcgagtg gcttaagcta tcatgtatta acgatgttac gtattgcaaa
     4741 atcaatttgt gacatttatc cattgttaaa caaaagtttg ttatatagtg gtattatttt
     4801 gcatgatatt ggtaaagtta gagaattgag tggtcctgtt gcgacgtcgt atacagtcga
     4861 aggtaattta ttaggacaca tctcgattgc gagtgatgaa gtagttgaag cagctcgtga
     4921 attgaacatt gaaggagaag aaatcatgtt gttaagacat atgattttat ctcatcatgg
     4981 taagttagag tatggttcgc caaagctgcc atacttaaaa gaagcagaaa ttttatgcta
     5041 tatcgataat atcgatgcta gaatgaatat gtttgaaaag gcttataaaa aaactgacaa
     5101 gggtcagttt acagataaaa tatttggtct tgaaaatcgt agattctaca atcctgaatc
     5161 actcgattaa gaattaaaag atatcggaca gatgagtata ctgaaacatg agctcatctg
     5221 tccgattttt aatgcgaaaa aaccgagcgg ccgtggtatg atttttaaac cacggtcgct
     5281 cggttttgtg ttattggctc atgccggatt gtccgccttg tgcgccacct tgtttaagtt
     5341 tttcagggtt taagatttta tcttcgacaa ctgatttaat atcacgatct ttaaagtcaa
     5401 catcgtattc ttttaataga tctttgtatg catcagttaa taattttgga tttttttgta
     5461 ctttctgatc gactaatttt tctttcaggc tttgtttttc actgttaaag tctgttggtt
     5521 tatcagcttt aataatatga tatccaaagc ttgatttaac aacgtctgat acttcaccat
     5581 ctttaagttt aaatagtgct ttttcaaaat ctttatcagt ttgtccttta agaacataac
     5641 ctaattcgcc atctttttta gctgaaccag tatccattga ttctttttta gcgatttcac
     5701 caaatttact tggatctttt gaaacttctt tttgaatttc ttcagctttt tgtttcgctt
     5761 ctttatcatc taagccttct ttgtcgcttt tcttagattt aactttaatt aaaatgtgtg
     5821 aagctttctt gctgtcttct ttaatttcag aatcagagat tttaatttta tctgatagta
     5881 attctttatg ataagcagca gtacgtaaat tttctttata tttatcggct gttaaacctt
     5941 gctgttgaag ggccttttta aatttatctt taccgccgta ttgcttttgc attttttcaa
     6001 tttgttcgtc aatcttctta tcattaactt tatttttata tttatcagct aaaattttat
     6061 ttaacatttc agtaaacgat gcatttgcaa tttgatcttt accgattttt ttcattgtat
     6121 ctgcaactgt tacatctcca gctttagaag aaattaatgt attttcttta gaatctgtgg
     6181 cactagcgcc acaagcgcct aataataaag cactagctgt taccggaacg attaatttgt
     6241 ttatcatctt catagttgaa actcctttgt aagttaatca tcgtgaatat aacatagttt
     6301 tatattatca ccaatcaata acccatgaca tcagactaga gcattaggtt taggggtaga
     6361 aattgatgaa aataaaaaaa caaggtctat atacatttga ttgtaagcta ttacatagcc
     6421 tgaaaacaaa taataacacc ttgctataat tattttgtta taaatgatat ggatttttaa
     6481 tttttttcaa aat
//
LOCUS       DAKSQE010000129         6211 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.129, whole genome shotgun sequence.
ACCESSION   DAKSQE010000129 DAKSQE010000000
VERSION     DAKSQE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.129"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            53..979
                     /locus_tag="O0V33_002331"
     CDS             53..979
                     /locus_tag="O0V33_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8976935.1"
                     /translation="MGKYIFKRFIYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQ
                     KEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLT
                     AMFIGVILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWF
                     PVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLF
                     GHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAIT
                     LLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK"
     gene            979..2049
                     /locus_tag="O0V33_002332"
     CDS             979..2049
                     /locus_tag="O0V33_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8976936.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAIASSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVQHRNLPA
                     KIPLLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            2065..3147
                     /locus_tag="O0V33_002333"
     CDS             2065..3147
                     /locus_tag="O0V33_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8976937.1"
                     /translation="MTERILELNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKLALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPIGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLKVERVSFNEK"
     gene            3137..4078
                     /locus_tag="O0V33_002334"
     CDS             3137..4078
                     /locus_tag="O0V33_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8976938.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFEIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQREKGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPADDIYQYPLHDYTKSLLSAIPQPDPESERSRKRFSYIDD
                     EANNHLRQLHEIRPQHFVFSTEEEAARLRKNLLVTQD"
     gene            4097..5752
                     /locus_tag="O0V33_002335"
     CDS             4097..5752
                     /locus_tag="O0V33_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCX8976939.1"
                     /translation="MTRKLKTLILIFVATIALSGCANDDGIYSDKGQVFRKILSADLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALD
                     DETLQIDLEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKKVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNELSFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
     gene            5964..>6211
                     /locus_tag="O0V33_002336"
     CDS             5964..>6211
                     /locus_tag="O0V33_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCX8976940.1"
                     /translation="MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTEASKHKG
                     GTLNVALTAPPSGVYSSLLNSTHADAVVEGYFNENLLT"
BASE COUNT         2237 a          875 c         1195 g         1904 t
ORIGIN      
        1 tttgaaggga atggaaattt agaaatcgga atttgttaga ggaggggatt agatggggaa
       61 atatattttt aaacgattta tttatatgct tatttcttta tttattatta ttacaattac
      121 atttttctta atgaaattaa tgccaggttc accatttaac gatgctaaat taaatgctga
      181 acaaaaagaa attttaaatg aaaaatatgg attaaatgat cctgtagcaa cgcagtattt
      241 acattattta aaaaatgttg ttacagggga ttttggtaat tcattccaat atcataatca
      301 acctgtgtgg gatttgatta aaccgagact attaccttct tttgaaatgg gtcttacagc
      361 aatgttcatc ggtgtgatac tgggtcttat tttaggtgtt gcagcagcta ctaaacaaaa
      421 ttcttgggtt gattatacaa ctacagttat ttcagttatt gcagtatctg taccatcttt
      481 tgtacttgct gtacttttac aatacgtatt tgcagttaaa ttaagatggt tcccagtagc
      541 tggatgggaa ggtttttcga ccgcggtatt accgtcactt gcattatctg cagctgtttt
      601 agcaactgtc gccagataca taagagcaga gatgatagag gtattaagtt cagactacat
      661 tttattagcg agagctaaag gtaattcgac aatgcgtgta ctttttggac atgcacttag
      721 aaatgcttta attccaatta ttacaattat cgttcccatg ttagcaagta ttttaacagg
      781 cactttaaca attgaaaata tttttggagt tcctggatta ggggatcaat tcgtacgttc
      841 aattacaaca aatgatttct cagtaatcat ggcaatcaca ctattattta gcacactgtt
      901 tatcgtttct atttttattg tagatatttt gtacggtgtg atagatccac gaattcgtgt
      961 tcaaggaggt aaaaaataat ggctgaaaat aaaaacaatt tgtcgattaa cgacgatcat
     1021 tctaatgcag ctatgacgca tacctctgac gctatcgcat catctgattt tattattaga
     1081 gaattagatt tgaatcagga acctgaaatg caacgagaaa gcaaaaactt ttggcaagat
     1141 gcttgggctc agttaaaacg aaataagtta gctgttgtcg gtatgatagg tttaattatc
     1201 attgtaatat ttgcttttat cggtccagtt ataaataaac atgattatgc tgaacaaaat
     1261 gtacaacata gaaatcttcc ggcaaaaata cctttattag acaaagttcc atttttacct
     1321 tttgatggta aagatgcaga tggcaaggat gcttataaag cagcaaatgc taaagaaaat
     1381 tattggtttg gtactgatca gttgggtcga gatttatgga caagaacatg gaaaggtgct
     1441 caaatttcat tgtttatcgg tgttgttgca gcgatgttag atatttttat tggcgttgta
     1501 tatggtgcga tttctggatt cttcggtgga cgtgtcgata cgattatgca acgtatactt
     1561 gaagtcatag catctattcc gaatttaatt gtcgtaattt tatttgtatt aatttttgaa
     1621 ccatccattt ggacaattat attggctatg tctatcacag gctggttagg catgagcaga
     1681 gttgtacgtg gagaattttt aaaattgaaa aatcaagagt ttgtcatggc ttcgaaaaca
     1741 ttgggggctt ctaaattcaa attgatattt aagcatattt tacctaatac gttaggtgct
     1801 atcgtggtta catcaatgtt tacagtacct agtgctattt tcttcgaagc atttttaagt
     1861 ttcattggta taggtgtacc cgcacctcaa acatcgttag ggtcattagt aaatgatggg
     1921 cgcgcaatgt tattaattta tccacatgaa ttatttatac cagcaatgat tttaagttta
     1981 ttaattctat tcttttactt atttagtgat ggattacgtg atgcatttga tccgaaaatg
     2041 cgtaaataaa agggggcata gcatatgact gaaagaatat tagaactaaa tgatttgcat
     2101 gtttcctttg atattacagc aggggaagtg caggcagtga gaggtgtaga tttttattta
     2161 aacaaagggg aaacattggc aattgttggt gaatcaggtt caggtaaatc tgtgacaaca
     2221 aaagcaatta caaaattatt ccaaggggac acaggaagaa ttaaaaaggg agaaatttta
     2281 tttttagggg aagatttagc aaaaaaacct gaaaatgagt taattaaatt acgtggaaaa
     2341 gatatttcaa tgatctttca agatccaatg acatctttaa acccaacgat gcaaattggt
     2401 aaacaagtca tggaaccatt aattaagcac aaaaattata gtaaagcaca agctaaaaag
     2461 ctcgcattgg aaatactaaa tcttgtagga ttaccaaatg cagaaaaaag atttaaagca
     2521 tatccacatc aattttcagg tggacaaagg caaagaattg ttattgcaac cgcattagct
     2581 tgtgaaccta aagtgctgat tgctgatgaa ccaacgactg cattagacgt aacgatgcag
     2641 gcacaaattt tagatttaat gaaagaacta caacaaaaaa tcgatacagc aattattttt
     2701 ataacgcatg atttaggggt tgttgcgaat attgctgata gagtggcagt tatgtatggt
     2761 ggtcaaatgg ttgaaacagg agatgttaac gaaatatttt atgatccaaa gcatccatat
     2821 acatggggat tattatcgtc aatgcctgat ctatcaacaa caaatgacac accattacta
     2881 gcgattcctg gagcgccacc tgatttatta cacccaccta taggtgatgc atttgcgaga
     2941 cgtagtcaat atgcattaga tattgatttt aaagtagaac cgccgtggtt taaagtttca
     3001 ccgacacatt ttgtgaaatc ttggttatta gacgcacgtg caccaaaagt tgaactaccc
     3061 gagctggtaa aacaacgtat gaaaccgatg cctaataatt atgaaaaacc actcaaggta
     3121 gaaagggtgt cgttcaatga aaaatgatga agtgctatta tctattaaaa atttaaagca
     3181 atattttaac gcaggaaaga aaaacgaagt gagagcgatt gaaaatattt cgtttgaaat
     3241 atacaaagga gaaacattag gtttagtagg agaatcgggg tgtggtaaat ctacaactgg
     3301 taaatcaatt atcaaactta atgatattac aagtggagaa attttgtatg agggtattga
     3361 tatacaaaag attcgtaaac gtaaagattt gcttaaattt aataaaaaga tacagatgat
     3421 ttttcaagac ccatatgcgt ctttaaatcc taggttaaaa gtaatggata tagtagctga
     3481 aggtattgat atccatcatt tagcaactga taagcgtgac cgtaaaaaac gtgtctatga
     3541 tttacttgaa actgttggat taagtaaaga acatgccaat cgttatcctc atgaattttc
     3601 aggtggacaa cgccaacgta ttggaattgc ccgtgcatta gccgttgaac cagaatttat
     3661 tatagcagat gaacctattt cagcattgga tgtttcaatt caagctcaag tagtcaattt
     3721 gttattaaaa ttacagcgtg aaaaaggtat tacattccta tttattgcac atgatttatc
     3781 gatggtgaag tatatctctg atcgaattgc agtcatgcat tttgggaaaa ttgtagaaat
     3841 cggaccagca gacgatattt atcaatatcc attacatgat tatactaagt ctttattgtc
     3901 ggcaattcct caaccggatc ctgaatcgga acgtagtcgt aaacgattta gttatattga
     3961 tgatgaagca aataatcatt taagacaatt acatgaaata agaccacagc attttgtctt
     4021 tagtactgaa gaagaagcgg cacgactacg aaaaaatttg ttggtaacac aagattaagg
     4081 ggaagggaga aatgaaatga caagaaagtt aaaaacgctg attttaatat ttgttgccac
     4141 aattgcatta agtggttgtg ctaatgatga tggtatttat tcagataaag gtcaagtatt
     4201 cagaaaaatt ttgtcagcag acttaacatc acttgataca tcattaatta cggatgaaat
     4261 atcttctgaa gtgactgcgc aaacattcga aggtttatat acattaggaa aaggtgacaa
     4321 accggtgtta ggtgttgcga aagcttttcc tgaaaagagt aaagatggga aaactttaaa
     4381 ggttaaatta agaagcgatg ctaaatggag caatggtgac aaagtgactg cacaagactt
     4441 tgtttatgct tggagaaaaa cagttgaccc taaaactggt tctgaatttg catacattat
     4501 gggggacatt aaaaatgcga gtgatattag tactggtaag aaacctgtag agcaattagg
     4561 tatcaaagca ttagatgatg aaacattaca aattgattta gaaaagccgg taccatacat
     4621 taatcaatta ttagcactta atacatttgc acctcaaaat gaaaaagttg ccaaaaaata
     4681 tggtaaaaat tatggtacgg cagctgatag agcggtatac aatggtccat ttaaagttga
     4741 tgattggaaa caagaagata aaacgttact atctaaaaat cagtattatt gggataaaaa
     4801 gaaagtaaaa ttagataaag tgaattataa agttattaaa gacttacaag caggtgcatc
     4861 attgtatgat actgaatctg tagatgacgc agttattaca gcagatcaag taaataaata
     4921 taaagataat aaaggattaa actttgtgtt aacgactggg acattttttg taaaaatgaa
     4981 tgaaaaacaa tatcctgatt ttaaaaacaa aaatttaaga ctggctatcg cacaagcaat
     5041 agataaaaaa ggatacgttg attcagtgaa aaacaatggt tcaattcctt ccgatacact
     5101 aacagccaaa ggcattgcga aagcacctaa tggcaaagat tatgcgagta ccatgaattc
     5161 acctctaaaa tataatccta aagaagcaag agcgcactgg gacaaagcta aaaaagagtt
     5221 aggtaaaaat gaattgtcat tttcgatgaa cacagaagat acaccagatg caaaaatatc
     5281 tgctgaatac atcaaatcac aagttgagaa aaatttacca ggagttactt tgaaaattaa
     5341 gcaattgccg tttaaacaaa gagtatcact agaactgagt aacaattttg aagcatcact
     5401 tagtggttgg tctgcagatt accctgatcc tatggcttat ttagaaacaa tgaccacagg
     5461 tagcgcacaa aataatacag actggggtaa taaagaatac gatcaattac ttaaagtagc
     5521 gagaaccaaa ttggcacttc aaccgaacga acgatatgaa aacttgaaaa aagcagaaga
     5581 aatgttccta ggagatgcac cggtagcacc aatttatcaa aaaggtgttg cacatttaac
     5641 aaatcctcaa gtaaaaggat tgatttacca taaatttggt ccaaataact cacttaaaca
     5701 tgtatatatt gataaatcga tagataaaga aacaggtaag aagaaaaaat aatttgcttt
     5761 gtaaattaga ctggagatat atctccagtc tttttgtgtt gcagaaaaac tttgggaata
     5821 aaaaattaaa ataagtcgtt ttttaaatta ctgaaattga ttaaatgcat aaatgactga
     5881 atattctaaa aataaacttg taataatttt ttctatgagt aaactaaaaa gaaaatatta
     5941 gattgaaagt aggaggcata tgtatgggga agcttattaa atatatttca atacttctta
     6001 ttgtcgtttt agtgttgagc gcttgcggaa aaagcagtaa taaagatgaa ggagtaaaag
     6061 atgctactaa aacagaagcg tcaaaacata aaggtggtac tctaaatgta gcgttaacag
     6121 caccgccaag tggtgtttat tcttcgttat taaatagtac acatgcggat gctgtagttg
     6181 aaggatattt taatgaaaac ttattaacaa t
//
LOCUS       DAKSQE010000130         6124 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.130, whole genome shotgun sequence.
ACCESSION   DAKSQE010000130 DAKSQE010000000
VERSION     DAKSQE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.130"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            220..1158
                     /locus_tag="O0V33_002337"
     CDS             220..1158
                     /locus_tag="O0V33_002337"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCX8976941.1"
                     /translation="MANIVFKLSDKDITTLMSRISFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSEELLPQHSQLNTNKTKKKNTANSSLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLT
                     LHNEKYNIQQAKGWTQVKMKAVLHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFITYMDQISKNINMTIPKGAGAKVD
                     VIAAKIIKNYGISRLDSISKKHFKNREKAQKILKPL"
     gene            complement(1460..3862)
                     /locus_tag="O0V33_002338"
     CDS             complement(1460..3862)
                     /locus_tag="O0V33_002338"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCX8976942.1"
                     /translation="MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIFVFSESQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELELSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQVRLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     HTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVQVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFEKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFAMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQGKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVIGFIGELHPTLAADNDLK
                     RTYVFELNFDALMAVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(3862..4920)
                     /gene="pheS"
                     /locus_tag="O0V33_002339"
     CDS             complement(3862..4920)
                     /gene="pheS"
                     /locus_tag="O0V33_002339"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCX8976943.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEEKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(5301..6041)
                     /locus_tag="O0V33_002340"
     CDS             complement(5301..6041)
                     /locus_tag="O0V33_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCX8976944.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQS
                     GIVITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPQYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
BASE COUNT         1989 a         1177 c          868 g         2090 t
ORIGIN      
        1 aaatgaaata atgtaaagaa ttttctattc aatactttac catgtttaaa ttgtgtcact
       61 ataacatttt cataaacatt atacatgacc actatgtatt ttgtaagtat ccgcaattaa
      121 ttctttacaa catatataaa tgtttctgac gttattatca tttatgatat gattattttt
      181 gctaatgata atgaaattat tacgaaagga tttacacaaa tggcgaatat cgtttttaaa
      241 ttgtcggata aagacataac gacattaatg tcacgcattt cttttgatac tgagaattta
      301 cctcaaggta tgaaagcacg tgcaaagtat caaaatacaa ctgtaaatat ttaccaatca
      361 ggtaaagtca tgtttcaagg taatcatgca gaagctgtgt ctgaagaact tctaccgcag
      421 cattctcaat tgaatactaa taaaactaaa aagaaaaata cggccaattc ttctcttgaa
      481 cagactttaa tgtatgatca atttaactgt attggtagtg atgaggctgg aagtggcgat
      541 tactttggtc cgttgactgt gtgtgctgct tttgttacca aagagcatgt ccctatttta
      601 aaaactttgg gtgtagatga ttcaaaaaaa ttaaccgata ctaaaattgt agaacttgct
      661 gagcaattgg ttactttcat cccacattca ctattaacac tacacaatga aaaatataat
      721 attcagcaag caaaaggctg gactcaagta aaaatgaaag ctgttcttca taatgaagcc
      781 atcaaaaatg tacttgaaaa aattgattcg tctcaattag attatatcgt cattgatcag
      841 ttcgcaaaac gcgaagtgta tagccattac gcattatctg atataccact tccaaagaaa
      901 acaaaatttg aaacaaaagg agaatcgaag tcattagcca ttgcagtggc aagcataatt
      961 tctagatacg catttattac atatatggat caaatatcaa aaaatattaa tatgacgatt
     1021 cctaaaggtg ctggtgctaa agtcgatgta atcgccgcca aaataataaa aaattacggt
     1081 atttcacgtt tagattccat ttcaaaaaaa catttcaaaa atcgtgaaaa agcccaaaaa
     1141 attttaaagc ctctttgatt tataatcatt agaggctttt atgcagttgt gcaaagcatc
     1201 aactgtaatc cgctgagcct ctatgattta taatcaagat aaattggatc ctaataggaa
     1261 atcctattag gtaagtcata ctaatttgct ttgttcataa gtatgacttt atatgcagtt
     1321 gtgcaaagca tcaactgaaa accactcttc ctctttgatt tataaatgaa atggaaccta
     1381 ataggtgaat ccaaaaataa aaccccatgt tagtgatatc aatctaattc agatatcctt
     1441 atacatgggg tttaaatcat tatctaataa cagcaccttg ttcaattaat gctgcttcaa
     1501 tttccgcttg tacttttgaa acgcgttcat ctgtcaatgt ttcttctgtg tctaaataat
     1561 ttaaacgtat tgcaattgat tttttacctt tttctaaatg ttcgccctga tatacatcaa
     1621 agacaagtgt atcttttaat atattgccac cgtgtgcatg aatcgttgat aataaatcag
     1681 ctgctggaat attttgatct acttctaatg caatgtcacg agacatgcct gggaatctcg
     1741 gaattggctg gtaattaatg taacctaccg acacagccat taatgcatca aaattcaact
     1801 caaaaacata cgtacgtttt aaatcattat cagctgctaa tgttgggtgt aattcaccaa
     1861 taaaaccaat aactttattc tctaataaga tttcagctgt acgacctgga tgtaatccat
     1921 caatatcagc acgacgataa ctaaattcaa ggtttaactt ttcagatact cgatccacga
     1981 caccttttgc taaataaaaa tcaaccgttt ctttcttacc ttgccattga ttgactacat
     2041 aatctccagt taaaatacca cttaaatatt caacttgatc tggtagttca ccttcaccat
     2101 tagcaaagaa gacattgccg atttcaaata attttacatc tttattttta cgtgccacat
     2161 tatatgatgc cgcttcgatt aaatgtggta ataaactttg acgtaatgac gcatgcgctt
     2221 cactcattgg catcaataaa tcaattgttt gacgctgttg catcgcaaat gcagtagcat
     2281 cttctttaga aactaacgaa tacgtaatag cttggtctaa tccagcacct tctaacactt
     2341 ctttaaccat tctcgtttta tattggcgat cagttaactg accactagta actttttcga
     2401 agacaggtaa tgttgaagga atatcatcgt aaccataaat acgtgcaact tcttcaatta
     2461 aatcttcttt aattgtaata tctttgcgac gtgacggcac ttgcactgta ataacatcat
     2521 catttatttc tgtatcaaac cctaattgat taaaaatagt aacaatatca ttttgtgaca
     2581 aatcaaatcc aatagtgcga ttaattttat cagcagtgat gtcgattggt gtaataaatg
     2641 caccaagttc tcctgaagac actctatctt ttagcacttt tccgtttgca taagtttgta
     2701 ataaataaca tgcacggtcg actgcttcat ctacaaattc agtagctatt cctttttcaa
     2761 aacgactaga tgattcactg cgtaaattta aacgtcttga tgtatgacga attgaaactg
     2821 gatcaaaaat agcaccttca ataactatat ttgatgtatg ttctttaact tctgaaaaat
     2881 cgccacccat aacaccagct aatgcaattg gagtttgtcc attagtaata acaatatcgc
     2941 tcgttaataa ttcacgttct gtatcatcta atgttgtcat tttttcgcct tcattagctt
     3001 gacgaacaac aatctgttgt gaaccaatcg catcttgatc aaacatgtgc aatggttgac
     3061 cgtattctaa taacacataa tttgagatgt cgacaacatt attaatagga cgtatacctg
     3121 cttttattaa gcgtacttgc atccaaattg gcgaaggttc aattgtcacg tcgtgaacaa
     3181 cacgtgcact ataatatggt actttatctt cattttcaat tgtcacagtc agttcatcat
     3241 ttgcagataa ctcaagctca tttgatgttg tctctggctt agtcattttt gtattatata
     3301 atgctgcaac ttcataagca gtacctatca tacttaaagc atcagcacga ttcggcgtta
     3361 aatcaaattc cattacttga tcatctaaat ataaagcttg taaggcatct gttcctggaa
     3421 cttgggattc actaaataca aaaatgcctg attcaaaact tttcggtata tagttacttg
     3481 aaataccaat ttcttgtaac gaacaaatca taccttccga acgttcaccg cgtaatttgg
     3541 cacgcttaat tttaatacca ccaggcaatc taccacctac tttagcaaca atgacatatt
     3601 gtcctgcatc aacgttcggg gcaccacata cgatttgtac aggttcgtct tctccgatat
     3661 caacttggca aacatttaat ttatcagcat caggatgttt ctcttttgac ttaacgaagc
     3721 caacaactaa atttttgata tcttttgtgt agtcaattaa atcatccact tcaatacctg
     3781 tgcgcgtaat acgttctgcc aaattactta cagaatcatc gattgttaca tattctttca
     3841 accattcatt tgatatcaac attacatgtc acctctatct tctaccgctt taaattgatc
     3901 taaaaatctc acatcattag tatagaaatg acgaatatct tctataccat atttcaacat
     3961 tgcaatacgg tctggtccca taccaaatgc aaatccagag tactctgaag aatcaaaacc
     4021 agccatttct aatacattag gatgtaccat tccagcgcct aaaatttcaa tccatcctgt
     4081 gtgtttacac acattacaac cttttccttt acatttaaaa catgacacat caacttctac
     4141 agaaggttca gtgaatggga agtaacttgg acgtaaacga atttcacgat cagcaccaaa
     4201 taatttctta gctaacaatt ctaaagtacc tttcaaatca ctcattttaa cgtttttatc
     4261 aacaactaat ccttcaattt gtgtaaattg atgactatgt gtcgcatcat cagagtcacg
     4321 acgatacact ttaccagggc aaataatttt aactggacct tgaccatgac gtgattccat
     4381 cgtacgtgct tgcactggtg atgtatgcgt acgtaataaa atttcatccg taatatagaa
     4441 actatcttgc atatcacgtg cagggtgtga tttaggtaaa ttcagcattt cgaagttata
     4501 atgatcttgt tcaacttcat atccattgac aatttcataa cctaaaccta agaataagtc
     4561 ttcaatttct tctattgtac gtgttaatgg atgctttgaa ccgatttcaa tatgacgacc
     4621 tggtaatgat acatcaattg tttcttcagc caattgctta tttaattttt ctttaactaa
     4681 catctgttgt ctttcatcta attcattttg aattgtttga cgcaattcat tcactttttg
     4741 accaaacgca ggcttctctt cattcggcaa atccttcatc aatttcatta gtccgctaac
     4801 tgaccctttt ttacctaagt atttcacttt aacttcttgc agtgcacgtt catcatttgc
     4861 ttcattaata tctacaagcg cttgttgttt taactctgac attgtttgtt gttcagacat
     4921 atgtcactga cctccctatt cgaactattc attttctatt tatcatattt gttgtttaac
     4981 taaaaaaaga ctttcatcct cttaaaaaat aagggacgaa agtcatttcc gtggtaccac
     5041 ccaaatttat gcctaagcat acacttgttt aatattgata acggattgtc tttccgactt
     5101 aaatctcttt aagtaaccaa aaaggtgaaa agactttaaa taattggaat aacttgcagt
     5161 cacgattatt ctccctaaaa caattattta tataaatgtc catgcctttt tattggttta
     5221 tattcaattg tgtatgcaat cacgtagaaa tgttctgtta aacaattaat tataattata
     5281 tcggaaaact ttcaacacgg tcaacctttc aaatgataaa gtaaaatact acctgcaatc
     5341 gctacattta aactttcagc tttaccataa ataggtatga ttaaattttg tgtagtatgt
     5401 gctaataatt caggattaac accttctccc tcattaccta atagtaatgc aaaagaatca
     5461 cttgaagtaa cttctttata tgccactgcg ttttcaagtg ctgtaccata aacaggacca
     5521 ttaaactgag taataaacgt atcgagatct tgtgtcataa ctggcaaatg aaaaacacta
     5581 ccttgactcg ctcgcaacac tttatcttga taaggatctg tcgtaccctt ttccattatt
     5641 acagcatcca ttccagcagc atccgcagtt ctaattaatg tgcctaaatt tccaggatct
     5701 tgaacacgat cgataagcaa tacttgttgt gctttagaaa tatcatattg tggcttctca
     5761 atgattgcga aaaacccttg tggtgtcact gtacctgata aagattcagc aactttcatg
     5821 tttatttcaa aaacttcttg cgcgtatgcg ataatttgct gatctaatct tgccggttca
     5881 attgcaaata attgtgtaat tacaatccca ctttgataag cttcttcaat taaatgcaca
     5941 ccttcaatta aagctaatcc agttttatcc ctctcacgtt tcttttttag cttgttcgct
     6001 tgtttaattc tattattttg tgcagaagta atttgttcca tagatagctc ctcgctttat
     6061 ttttaaaaat aaaaatatta atcattaata agatgaaaac atttgattgt atagttaata
     6121 ttaa
//
LOCUS       DAKSQE010000131         5874 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.131, whole genome shotgun sequence.
ACCESSION   DAKSQE010000131 DAKSQE010000000
VERSION     DAKSQE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.131"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            91..755
                     /locus_tag="O0V33_002341"
                     /pseudo
     CDS             91..755
                     /locus_tag="O0V33_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270135.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            761..1768
                     /locus_tag="O0V33_002342"
     CDS             761..1768
                     /locus_tag="O0V33_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCX8976945.1"
                     /translation="MNRKTPKTTDGLMRHIRDNKGIQINGSTEKNQLRNIGYFHGFKG
                     YNFFLNKEEELNFEKFSELHALYSFDTEIKNLFYKHVMFCETAIKNRLLEIVCVNSGF
                     DLDSLFQKSLTYYKSYSPGSSKYKKHLKNTTRLRRDIYDMIHEHCNSKPYIYHYIHQD
                     RTVPLYAVFELFTLGNLVFFTRCMSQNLNIEAQKQLKLYSPAFDQSKDILGDIIDFMK
                     GLRNAIAHNGVLYDCRFKTGETSNRLKEYLAVKMDIHNLDFDRIIDYLILLILICSGL
                     SYSKAELQRIIRQFEQNLDFLRSKISESTYDKIIGVRARPKLRSLKNFINNLDFYLKT
                     D"
     gene            complement(2727..3239)
                     /locus_tag="O0V33_002343"
     CDS             complement(2727..3239)
                     /locus_tag="O0V33_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976946.1"
                     /translation="MKQQMLSKVLLSTVVVMGSIAGSSLVMDDNAHAEQKSDNIGKLN
                     QKNESTLHLSFEKGIKGTVDKNGKLTLSDGKTSKVMPTNAKDKKGNDVVLVYKKVKDG
                     FDVQVIKSSQERKTNWVKCGLGTVGGAGTGGLGGASAASVIPGLGTVAGAIIGGVSGG
                     ATGAAASCFG"
     gene            complement(3357..3794)
                     /locus_tag="O0V33_002344"
     CDS             complement(3357..3794)
                     /locus_tag="O0V33_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HCX8976947.1"
                     /translation="MAGQSIFETGRRLKHVKENDLAHGEFGKWLEKVGLDKYQASRFI
                     KVANEQSKLHSSANLGLKALYQIATIPVEHREEKQQTSSGEMKTPYEMTNKEREEFKR
                     QLKQRDEENAQLQSQMEQAQRSEEIARKQYKYGLNNYIFTIKF"
     gene            complement(3936..4904)
                     /locus_tag="O0V33_002345"
     CDS             complement(3936..4904)
                     /locus_tag="O0V33_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCX8976948.1"
                     /translation="MKPFESHNKQLKILRRRGMEVPSSAKRDLENENYYNIINGYKDL
                     FLELDVNGNFLVPDKYKQGTHFKEVFSLYKLDRKFRNVLLEYLLVFETHIKSRISYYF
                     SEKYREPHSYLYFKNYSSDTSKTDSIVKMVATFSSVMSNRKNKPLKHYINTHNGVPLW
                     ILVNYLTLGNVSKMYSNLDDDLRLEVAKDYKRKLERDYKTRVQITPSDVDSILQQAHM
                     FRNVCAHEERLYDYKIDRAKSRANIFANYNKIYDKEYVPTMNGSYVFDLLISLCLFLN
                     KHDYIKLVKNMDKLISNYSHSFYTITIDDLYTKMNFPDQTKILDML"
     gene            complement(5181..5750)
                     /locus_tag="O0V33_002346"
     CDS             complement(5181..5750)
                     /locus_tag="O0V33_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCX8976949.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYSANSAHVTGCRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNAAVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKARDMLGKYHKLFTDKHDINGDVPIFINIGEWDGD
                     DEELDKTVKDVSNANPNHTVIVDDIPLED"
     gene            complement(5747..>5874)
                     /locus_tag="O0V33_002347"
     CDS             complement(5747..>5874)
                     /locus_tag="O0V33_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCX8976950.1"
                     /translation="IFDSLIQGRSYKDISSVLECSEQSVRLWYETLLDKIVEVIE"
BASE COUNT         2000 a         1058 c          773 g         2043 t
ORIGIN      
        1 ataaaatttg ttaatttaaa atagtaagca attcaaagtt atatgtgtaa tagataaaat
       61 agatatccct atagtgatgc gttactaact atatgtaata acaatttaaa agataatgta
      121 gtgaaagaaa taccggattg tttcgactat ctaattcagc ataaaaatac ccaacgagaa
      181 aacgtaatta taaaaaggta aaatctatag tgcagtttca aattgctaat ggcatgcgta
      241 tcggtgagct acttgcaata aaaagagaaa atataaacta tgaagataaa acgctagata
      301 tcgatggtac aattaattgg ataactgatt aaaaatggaa gcatccggag taaaagagac
      361 aactagaaca agtaacagct ataggactat aggcctcact atccatagca tcgacttact
      421 aagaacactt atgctttgaa tgataagttt attaatatag ggtacatatt cacaaatgca
      481 gctggtggtc ctatcgattt aaacaaaata aataacatta ttaaaggggg ggctatcaaa
      541 gagacaactg agattagttc tattaagaaa cctgcaacga cgcatacatt acatcattcg
      601 catatatcta cacttgctca attaggaatt aacttaaaag caatgcaaga gcatgtaggt
      661 cattcagatt ataaaaaaat ctagagatat acacacatgt tactaatcag atggcgaaag
      721 atatgctgaa taaatttgaa cgattgggga gttaaaaact atgaacagaa aaacacctaa
      781 aactactgat ggtttaatga gacatataag agacaataaa ggtatccaaa taaatggtag
      841 cacggaaaaa aaccagttac gaaacatcgg ctattttcat ggattcaaag ggtataattt
      901 ctttttaaac aaagaagaag aacttaattt tgaaaaattc agcgaattac acgctttata
      961 cagttttgat acagaaatta aaaacctttt ctataaacac gttatgttct gtgaaacggc
     1021 tattaaaaat agattgctag aaatagtttg tgtaaactct ggctttgatt tagatagttt
     1081 atttcaaaaa tcattaacat attataaaag ttatagtccc ggtagttcaa aatataaaaa
     1141 acatttaaaa aatactacta gactaagaag agatatttac gacatgatcc atgaacattg
     1201 taattcaaaa ccttacattt atcattacat acatcaagat agaaccgtgc cactatatgc
     1261 cgtatttgaa ttattcactt taggaaatct ggtatttttc actcgttgca tgtcccaaaa
     1321 tttaaatata gaagctcaaa aacaactaaa attatactcc ccagcatttg atcaaagtaa
     1381 agatatacta ggtgacatca ttgattttat gaaaggctta agaaatgcta tagctcacaa
     1441 tggtgtactt tatgactgta gatttaaaac tggagagaca agtaatagat taaaagaata
     1501 tttagcagta aaaatggata tacacaattt agatttcgac agaataattg attatctcat
     1561 tttattaatt ctaatttgtt ctggtctctc ttattctaaa gcagaactcc aaagaattat
     1621 aaggcaattc gaacaaaacc ttgatttttt acgatcaaag ataagcgagt cgacttatga
     1681 taaaattata ggcgttcgag caagacctaa attaagaagt ttaaagaact ttatcaataa
     1741 ccttgatttt tatctaaaaa cagattaata ttatacataa atagcggtgc atatctgcgg
     1801 aatgcacttg aggttaactg ttactgacgg ttgcctcttt ttttatgcat ctaaattcat
     1861 attatttttg caatataaac atatctttgt gcaaattccg aacacaaaac attcacatcc
     1921 tcttttttgc ccttcatctt ttatcccaaa acacaaaaac aaccccataa gcctatgctt
     1981 acggggtttt acaataaact aaatattatt gttcttcttt tatatactgc tattttatga
     2041 ataatcatta gtgttcaaaa agctgttaaa tcaatgtttc atactcatat tgttcatttt
     2101 gaaagtctat gaacattcac actatttcgt aactttgcga actttttgcg aacatactac
     2161 ccttgcccct agtttcacaa ttacaccttt tttcgccttt ataacaacca cactcctaga
     2221 tcaataggtt gtaaggtttg aatgtaccta tagcactgaa cattttaatg ggctatactt
     2281 ctcaacaatt cacatcactt cataattaac gtattgcaat atgtattcac cgcctgcaaa
     2341 gtatgaggtc atatttttat atcaatgtac aaaaaactgt tttgccctta aaacaaccac
     2401 attcctgatt aataggtagt ttagtttgag cattttataa ttaacataaa aaatagacaa
     2461 gtaccgaagt acctgcctta tttctaaatc cacaggaatt cattaatata ataattataa
     2521 ttgtaactta attaatgtct attatagtta tatataatag aagcaaaacc taaaatgact
     2581 ataaaagcta tcaaaacata aactgaaaaa ttatcagttc taatataaaa cttaaaaagt
     2641 aagaacatta caaaagcaac tataaaatag acaggaataa acgactttcg ccaaagtata
     2701 gagtttttca tgaattcact ctcctatcaa ccgaaacatg acgctgcggc acctgtggca
     2761 ccaccagaaa ccccaccaat aatagcacct gcaacagttc ctaaacctgg tataactgaa
     2821 gctgcactag caccgcctag cccaccagtg ccagcgcctc caactgttcc tagaccacat
     2881 ttaacccagt tagtttttct ctcttgacta gatttaatta cttgaacatc aaatccatct
     2941 ttaacctttt tataaaccaa aacaacatcg ttaccttttt tatctttagc attagttggc
     3001 atcacttttg acgtttttcc atcagataat gttaacttac catttttgtc aacagtccct
     3061 ttaataccct tttcaaatga aagatgcaag gtactttcat ttttttgatt cagtttcccg
     3121 atattatcac ttttttgttc agcatgagcg ttgtcatcca ttacaagaga agatcctgct
     3181 attgatccca taactacgac tgtacttaat aatacttttg ataacatttg ctgtttcata
     3241 ataaaaactc cttttaaaat ttttacattt taattttatc agttcttact ataacgcttt
     3301 accaaaattt cttaagtgca ataaaatcat tcctaattgt aaaaaatggc gtgtgtctaa
     3361 aattttatag taaaaatata attatttaat ccatatttat attgctttct cgctatctcc
     3421 tccgaacgtt gtgcttgttc catttgtgat tgaagttgtg cgttttcttc atcgcgttgt
     3481 ttgagttggc gcttaaattc ttcacgttct ttattggtca tttcgtatgg tgttttcatc
     3541 tctcctgaag acgtttgttg tttttcttct cgatgctcta ctggaatagt tgctatctga
     3601 taaagcgctt taagtcctaa atttgcgctc gagtgcaatt ttgattgttc atttgcaact
     3661 ttgataaacc tgctagcttg gtacttatct aacccaactt tttcaagcca cttaccaaac
     3721 tctccgtgcg ctaagtcatt ttctttcaca tgcttcaatc ttcggccagt ctcgaatatc
     3781 gactgaccag cgatgtttga agtcgtattt cacgacgtca cacttacgat tagcatatct
     3841 agttacagaa tcttatcccc cactgcaaca cagggcgttt ctcagcgtct taaaataaaa
     3901 aaacgccact cgtaagtgac attaaaaaat atcttttata acatatccag tatttttgtt
     3961 tgatctggaa aattcatttt tgtatatagg tcatctatag taattgtata gaaagaatgt
     4021 gaataattac ttattagttt atccatattt ttcaccaatt ttatgtaatc atgtttattc
     4081 aaaaatagac atagtgaaat caacaaatcg aatacataac taccattcat tgtaggaacg
     4141 tattctttat cgtatatttt gttataattg gcgaatatat tagctctact tttagccctg
     4201 tctattttat aatcatacaa tctttcttca tgcgcacaca cgttacgaaa catatgtgct
     4261 tgttgtagta tactgtctac atctgatgga gttatttgaa cacgtgtttt ataatctctt
     4321 tccaatttcc ttttatagtc tttagcaact tccaatcgaa gatcatcatc caaattggaa
     4381 tacatttttg aaacattacc taaagttaaa taattcacca atatccatag tggcactcca
     4441 ttatgagtat taatataatg ttttaatggt ttattttttc tattactcat aaccgagcta
     4501 aatgtagcaa ccattttcac gatgctatct gtcttacttg tgtcagatga ataattttta
     4561 aagtataaat atgaatgtgg ttctctatat ttttcgctaa aataatatga aattcttgat
     4621 ttaatatgag tttcaaatac taacaaatac tctaataaaa cattcctaaa ttttctatct
     4681 agtttgtata aagaaaagac ttctttaaaa tgagtacctt gcttatattt atcaggaacc
     4741 aaaaaattac cattaacatc tagttctaaa aataaatctt tataaccatt tatgatatta
     4801 taataatttt cattttctaa atctctttta gcactactcg gtacttccat tcctcttctt
     4861 cttagaattt tcaattgttt attatgactt tcaaatggct tcataaattt acccctataa
     4921 aagtagccat aacccgaata gagttatggc tagatcgtta tatatataat acatttaatt
     4981 ttatcggttg taaataaata agaaataaca aaaagagata ttttacacaa ttacctcctt
     5041 tattttattg ctactcctca aacccaccaa tattatcaat aaacactggt gttgttacat
     5101 ttaagtctac tttctcagta aataagctat ggtatcaaac taattagtat taatttataa
     5161 atagcatagc ttcattttct tcaatcctct aacggtatat catccacaat cacagtatgg
     5221 ttaggattag cgttagatac atcttttaca gttttatcta attcctcatc gtctccgtcc
     5281 cattcaccaa tgttaatgaa tataggaaca tccccgttga tatcatgctt atctgtaaat
     5341 aacttatggt atttacccaa catatcacga gcttttaaac gatcacttgg cttaattggc
     5401 acctctatca gttcaacatg ttcattatag actaactgta ctttgccact ttgtggattc
     5461 tctttatatt ccccacgctt gaccacaact tctttcgttt ctgtttcgtc accgactgcc
     5521 gcattcgtaa gcacatgtag taactctttt gcagttaata cattctcatc tataatctta
     5581 tctttttgtt cttgtatata ttgcttgatg tgtggcttct ttaataacct acaccctgtc
     5641 acatgtgcac tatttgcgct ataacctgct tttatggcac tttgtgtcac attaagtgtt
     5701 cttatatact cattcacaaa acgcgcttgc tttgccgtta actcactcat tctatcacct
     5761 ccacaatttt atctaataag gtttcatacc ataatcttac agattgttct gaacactcta
     5821 agacactact aatatcttta taactacgtc cttgtattaa agaatcgaaa atat
//
LOCUS       DAKSQE010000132         5635 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.132, whole genome shotgun sequence.
ACCESSION   DAKSQE010000132 DAKSQE010000000
VERSION     DAKSQE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.132"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..312
                     /locus_tag="O0V33_002348"
     CDS             <1..312
                     /locus_tag="O0V33_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCX8976951.1"
                     /translation="TYKELATYLKILSDSSRLEILDLLSCGELCACDLLEHFQFSQPT
                     LSYHMKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCICHNLKTGE
                     C"
     gene            312..1604
                     /gene="arsB"
                     /locus_tag="O0V33_002349"
     CDS             312..1604
                     /gene="arsB"
                     /locus_tag="O0V33_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HCX8976952.1"
                     /translation="MMTTLATFIFLVTLLFVLWQPKGLDIGFTALAGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGSGLKMFVYIIL
                     LGAVVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFNISFSQYLSRMIIPNLFSLLASLLVLWLYFRKAIPKSFDANHIKKPID
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLILARKSNAVNIKQV
                     IKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFFTLLGLYLTLILF"
     gene            complement(1691..2545)
                     /locus_tag="O0V33_002350"
     CDS             complement(1691..2545)
                     /locus_tag="O0V33_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCX8976953.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
     gene            complement(2821..3045)
                     /locus_tag="O0V33_002351"
     CDS             complement(2821..3045)
                     /locus_tag="O0V33_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976954.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
     gene            3244..3714
                     /locus_tag="O0V33_002352"
     CDS             3244..3714
                     /locus_tag="O0V33_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCX8976955.1"
                     /translation="MKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQI
                     YKPSSNQSLSSFLFMRLNFYLIDLFRQQKRLNDIILCENHSPILTEQPTYFNEYDLRL
                     QDVCALLNHRERQWLMLYLEGYKQYEIADIMSLSTSTIKLIKASVKRKCQRFFK"
     gene            3827..4270
                     /locus_tag="O0V33_002353"
     CDS             3827..4270
                     /locus_tag="O0V33_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCX8976956.1"
                     /translation="MQDISTKHLLYIQTATSNHLETKCVFLYHEYNLEFPITKAIPYY
                     AKLHFTSASILIDAAKSILNINKLVPIYLNPKTILFPIKQKRAPIQTYINAHYIIGMT
                     EMGNSTMLHFKNGKTLEVDEPMSLISKKCYESLALKHFIENTNDY"
     gene            complement(4257..4700)
                     /locus_tag="O0V33_002354"
     CDS             complement(4257..4700)
                     /locus_tag="O0V33_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976957.1"
                     /translation="MNKASFDKKVKKQLWFLNKNEKQALDQRLSSITDKDNVNFNKPI
                     TFANTYLRENVFRSKETKSYSIFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVNPK
                     VELSTTVVILTIIGAILLMLVSIYLIKITTSYFTKKLLEHKFNSH"
     gene            4998..5633
                     /locus_tag="O0V33_002355"
     CDS             4998..5633
                     /locus_tag="O0V33_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCX8976958.1"
                     /translation="MQKFKDLFYDDLSVARGNYFLTLLSAFLITIILLIGFVVSDTHI
                     LYGVFIVLIGIVLLRLFKINLLSFKKLTMSQVIYIIGGALLIYGLDHLYLHFHDVPTN
                     EQELDRELQHMPLYMSIVTIAIIPAIVEEIVFRGMIIRVVFRKHLFIGLVVSSLVFAS
                     LHESDTWIGYLPYLYSGVIFGLIYLKTKRLEVVILIHFINNLSSLLILIWG"
BASE COUNT         1847 a          923 c          765 g         2100 t
ORIGIN      
        1 acgtataaag aactagcaac atatttaaaa attttatcag attcaagcag attagaaata
       61 ctagatttac tttcttgtgg agagttatgc gcttgtgatt tgttagaaca ttttcaattc
      121 tctcaaccca cacttagcta tcatatgaaa gcattagtaa aaaccaactt agttacgaca
      181 cgaaaaatcg gaaataaaca tttataccag cttaatcata atatttttga gtccgtaatt
      241 aataacttgt caaaagttca tacctctaac caacgatgta tttgtcataa ccttaagact
      301 ggtgaatgct aatgatgaca actttagcga cattcatttt tctagtaact ttattatttg
      361 tattatggca acctaaaggc ctagatattg gatttactgc attagcaggt gcctttattg
      421 ctgttattac tggtgttgta agtttttccg atgttttcga agtaacaggt atagtctgga
      481 atgccacttt aacttttgta tcagtcattc ttatttcatt aatattagat aaagttggat
      541 tattcgaatg gtcagctatt cacatgcttc atgcttcaaa aggtagtggc ctaaaaatgt
      601 tcgtttatat catattattg ggtgccgtgg ttgctgcatt tttcgcaaat gatggcgcag
      661 ctttaatctt aacgcccatt gtattagcaa tggtaaaaaa tataggtttt agtaagcggg
      721 ccatatttcc atttattatt gcaagtggtt ttattgctga tacaacgtct ttacctttaa
      781 tcgtgagcaa tttggtcaat atcatttctg cagattactt taacattagt ttctctcaat
      841 atcttagtag aatgattatc cctaatttat tctcgttatt agctagcctt ctagttttgt
      901 ggttatattt tagaaaagcg attcctaaat catttgatgc aaatcacata aaaaaaccta
      961 tagatgccat taatgattta aagctattta aaatttcatg gattgttcta gttatattac
     1021 ttttcggcta tctaatcagt gaatttacta aaattccggt atcaattttc accggaatca
     1081 ttgcttttat atttctaata ttggctcgta aatcaaatgc tgtaaatatt aagcaagtca
     1141 ttaagggcgc accttggaat atagtattat tttcaattgg tatgtatatc gtcgtattcg
     1201 gcttaagaaa tgctggcatt actttaatat tagctaaaat attagaatat atttccaatt
     1261 acggtctatt tagcactatt ttgggaatgg gcttcatttc agcattttta tcatcaataa
     1321 tgaataatat gcctacagtt ttaatagatg cgattgctat tggtcaatca aatgtccatg
     1381 gcatgttaaa agaaggccta atttatgcaa atgttatcgg ttctgattta ggtccaaaaa
     1441 ttacaccgat aggctcttta gctacattac tgtggttaca cgtcttaaca caaaaagatg
     1501 ttaagatttc ttggggcaca tactttaaaa ctggtatcat cattacaatt ccagtactat
     1561 ttttcacact tttaggatta tatctaacac ttatactatt ttaagtaata aaaagacgct
     1621 ttctatttat tgcgatagaa agcgtctttt tgtacttgag caagtcatat cataacttaa
     1681 ctctaacaaa ttacttattc aaatgtttac tgtcatcttt atacacaaag tatttgaagt
     1741 attttccttc agtcttcatg ttcttataaa agtcagcgat aattgttgca ttactttctg
     1801 cccacttaat atctgtagca tattgatgtt ctcctggatt ttttggattc catctcatac
     1861 tatacaatgt attttgatct gtgcttgata agaagtgctt atgaatgaaa tcagcaccgc
     1921 ctgaaatagc tttttcaggt gtatcccaac catgcttttt agcatattct gcacctgttt
     1981 taattgggtc tttatcaaga gctcccactc catagaaatt gtagtatttt ttaccatcaa
     2041 tttcgacacc attagctaat tcacttttaa ctgcgccagt ttctaataat gcatgtgaaa
     2101 ttaaataaac ttcgttaacg tgcttatctt tagcagcttt taagaaatca tccgtatgtt
     2161 tcaataacgt tggtctatcg actaacatac gtttaattct atttttatca atcccttgat
     2221 actttgataa atctaaaaat tgatattttt gcttttcatt atcgataaaa gtaccgctat
     2281 ccattgcact tttaatttca gttgcagatg catctctcca tgcatcattc tttttatttg
     2341 atacctgttg actcgtataa ttatttattt gtttctttgc tgcatcgttt aatgtaacat
     2401 ttaacttttc aatcttaatg tcggatttaa catgtttgaa aaatatctga tcggatatca
     2461 ttgagaaaaa taaaaatgag acaacagcaa atatgacaac aagtcctatt attccaaaaa
     2521 tagaaccttt cttgtgttta ttcatatcca cacctcttag gtcattgttg ttatacattc
     2581 agtttgacta cgataataaa gtttaacatt attaaaaccg tttaacaatt ttatctatgc
     2641 attgttacat attcataaat aaacagtaat gtaaatgaaa cgttttaaac gatttatcaa
     2701 cattacttta cgttgattca cgaatcataa atgttttatc tttttgtata acctttgaaa
     2761 acctttatat caataatctt attattcacg tattaaaaaa caaaaaattc tcttgttcgt
     2821 ttacttagtt tcttttttat aatccatcat aataaaactt gctgcatttg atctattttt
     2881 ataatagtac tgatttaaat caattgtacc ttcaatttga ccatcacgat acatcatttc
     2941 attagtgaag ttatttatca tcacgaaatc agcgcgatct cttataacat ctaaatcgtc
     3001 gtttcgtgca aaaaaatgtt ctaaatttaa atgtgcgtaa tccattgtta cctcccgatt
     3061 acaaattttg ttctatctac tatttagcaa agtaataata catcatatca cattaaatta
     3121 attacttatg tcttatattt aacacttaat ttagcgatta cgcaaatgaa tgatttttct
     3181 aaatatttta ttctatcttt atccggaaat atttgttata cgtataactt ggagaatgac
     3241 tacttgaaat ttaatgacgt atacaacaaa caccacaaaa tcatacacca tcttttaaaa
     3301 aaatataata ttagctataa ttatgatgag tattatcaac tactcttgat aaaaatgtgg
     3361 caattgagtc agatatataa accctcaagc aatcaatctt tatcctcttt tttattcatg
     3421 cgactaaact tttaccttat cgatctattc agacaacaaa aacgtttaaa tgatatcatt
     3481 ttatgtgaaa atcattcacc aatactaact gaacagccaa catactttaa tgaatatgac
     3541 cttcgtttac aagatgtttg tgccctacta aaccacaggg aacgtcagtg gcttatgctt
     3601 tatcttgaag gctataagca atatgaaatt gctgatatta tgtcattatc aacttcaaca
     3661 attaaattaa ttaaggcatc agttaagcgt aaatgccaac gtttttttaa atagatgcga
     3721 cgtgtcgtgt tgattaaaat gattaatact cttggaacgc tcaataatat gcatttcatt
     3781 tatgcgaacc attttattat taactgattg aaaaggagta ctatatttgc aagacatttc
     3841 tacaaaacac ctactttata tccaaaccgc aacttcgaac catcttgaga caaaatgtgt
     3901 ctttctgtac catgaataca atctcgaatt ccctattact aaagccattc cttattatgc
     3961 taaattgcat tttacatcag catcaatttt aatcgatgcg gcaaaaagca tactcaacat
     4021 taacaagctg gtgcctattt accttaatcc gaaaacgatt cttttcccaa taaaacaaaa
     4081 gcgtgcaccg atacaaacat atatcaatgc acactatata attggtatga cggaaatggg
     4141 caattcgact atgctccatt tcaaaaatgg caaaacatta gaagtcgatg aacccatgtc
     4201 tcttatttcc aaaaaatgct atgaaagttt agcattgaag catttcattg aaaatactaa
     4261 tgactattaa atttatgttc taacagtttc tttgtaaaat atgatgttgt gatttttatt
     4321 aaatagatac taactaacat taataaaatc gccccaatga ttgttaaaat aacaaccgtt
     4381 gtcgacaatt ctactttagg gttaacgaaa aattgcacac cagatagact tgtaattaat
     4441 ccaaataaaa acaaacctaa taaagcaacg tatgcaaaca tcatcaccac taatgttaca
     4501 aagatgctat aacttttagt ttctttacta cgaaatacat tttctcttaa gtacgtatta
     4561 gcaaaggtga ttggcttatt aaagttcaca ttgtccttgt cagtaatact agacaaccgt
     4621 tgatctaatg cctgtttttc atttttgttc aaaaaccata attgcttttt aaccttttta
     4681 tcaaatgatg ctttattcat aattaaggat ttctctcctt tataatattg acgtaatttt
     4741 aaacattagc gtcaattgaa agtgtatttt atatatttta cagaaataca tgcatcaaat
     4801 ctacaaaaat taactttgca attttatttt tagtaatttg acactgtata attgacaccg
     4861 tttatattgc aaacaaatgg ttttagttaa gtgtaatgat gcgcctttaa acatatctaa
     4921 gtttctcata taatgaaaat gaccatacca atgaaatgct ttaatattta ttttaaagat
     4981 gatagaaagg attaaatatg cagaaattca aggatttgtt ttatgatgat ttatcagttg
     5041 cacgtggaaa ttattttcta actttattat ccgcattctt gattactatt attctactaa
     5101 ttggtttcgt tgtcagtgat acacacatac tttatggcgt gtttatagtg ctcatcggta
     5161 ttgtgttatt acggttattc aaaattaatt tactctcatt taaaaaatta acaatgtcac
     5221 aagtgattta tatcatcggt ggtgcattgc taatttatgg gttagatcat ctttatttac
     5281 attttcatga cgtaccgaca aatgaacaag aattagatcg agaattacaa catatgcctt
     5341 tgtatatgtc tattgtcaca attgctatta tccctgccat tgtagaagaa atcgtttttc
     5401 gaggcatgat aataagagtt gtatttagaa agcatttatt tattggatta gttgtgtcta
     5461 gtctagtgtt tgcatcattg catgaatcgg atacttggat tgggtactta ccttatttat
     5521 attctggagt gatttttggc ctaatatatt taaaaacaaa gcgattagaa gtcgttattt
     5581 taatccactt tataaataac ctatcctcat tactcattct gatatgggga taata
//
LOCUS       DAKSQE010000133         5628 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.133, whole genome shotgun sequence.
ACCESSION   DAKSQE010000133 DAKSQE010000000
VERSION     DAKSQE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.133"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            58..840
                     /locus_tag="O0V33_002356"
     CDS             58..840
                     /locus_tag="O0V33_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCX8976959.1"
                     /translation="MGNIKSFALYISILLLIVVVAGCGKSDKTKEDSKEEQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTLLTSFSKSNKPDEIDDEGMVLYLNR
                     NTKKATGYYFVNKIYDDISKNQNEKKYRVELKNNKIVLLDNVEDEKLKQKIENFKFFS
                     QYADFKDLKNYQDGSITTNENIPSYEAEYKLNNSDENVKKLRDIYPITTKKAPILKLH
                     IDGDIKGSSVGYKKIEYKFSKVKDQETTLRDYLNFGPSDEDS"
     gene            888..1661
                     /gene="lpl1"
                     /locus_tag="O0V33_002357"
     CDS             888..1661
                     /gene="lpl1"
                     /locus_tag="O0V33_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl1"
                     /protein_id="HCX8976960.1"
                     /translation="MGYLKRFALYISVMILMFAIAGCGKGNETKEGSKETQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDGEFEKGDKGMWTIYTDFAKSNKPGELDDEGMVLNLDR
                     NTRTAKGHYFVTTFYRNGKLPDEKNYKIEMKNNKIILLDEVKDDKLKQKIENFKFFGQ
                     YANLKELKKYNNGDVSINENVPSYDVEFKMSNKDENVKQLRSRYNISTEKSPILKMHI
                     DGDLKGSSVGYRKLEIDFSKRENSKLSVIEFLSYKPAKK"
     gene            1692..2494
                     /locus_tag="O0V33_002358"
                     /pseudo
     CDS             1692..2494
                     /locus_tag="O0V33_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            2532..3302
                     /locus_tag="O0V33_002359"
     CDS             2532..3302
                     /locus_tag="O0V33_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCX8976961.1"
                     /translation="MGYLKRIGMCISLLIVIIFVTSCGGGNKITGDSKETQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDGEFEKGDKGMWTIYTDFAKSNKSDELDDEGMVLNLDR
                     NTRTAKGYYFVKKFYEKDKFSDRKNYKVEMKNNKIILLDKVNDPNLKERIENFKFFGQ
                     YANFKDLENYNNGDVSINWNVPSYDVEYKMSNKDENVKQLRSRYNIPTDKAPMLKMHI
                     DGDLKGSSVGYKRLEIDFSKEDRDISVIDYLSYKPAKK"
     gene            3334..4134
                     /locus_tag="O0V33_002360"
     CDS             3334..4134
                     /locus_tag="O0V33_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCX8976962.1"
                     /translation="MRYLNRVVLYIIVMVLSVFIIGCDKSSDTSEKPKEDSKEAQIKK
                     SFEKTLDMYPIKNLEDLYDKEGSRDGEFKKGDKGMWTIYTDFAKSNKQGGLSNEGMVL
                     YLDRNTRTAKGHYFVKTFYEKDKFPDRKKYKVEMKNNKIILLDKVEDPKLKKRIENFK
                     FFGQYANLKELKNYNNGDVSINENVPSYDAKFKMSNKDENVKQLRSRYNIPTDKAPVL
                     KMHIDGNLKGSSVGYKKLEIDFSKGEKSDLSVIDSLNFQPAKVDEDDE"
     gene            4153..4947
                     /locus_tag="O0V33_002361"
     CDS             4153..4947
                     /locus_tag="O0V33_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCX8976963.1"
                     /translation="MKSIKRIGLCISLLILIIFVTSCDGDNKITGDSKEEQIKKSFAK
                     TLEMYPIKNLEDLYDKEGYRDGEFKKGDKGTWVIRSEMKIQLKGENLESRGVVLEINR
                     NTRTAKGNYIVREVVEDSDGMTHNHTKRYPVKMENNKIIPLKQIVDEKVKKEIEEFKF
                     FVQYGNFKELENYKDGEVTYNPEAPIYSAQYQLKNSDYNVEQLRKRYNIPTQKAPKLL
                     LKGSGNLKGSSVGYKNIEFTFVENKGENIYFTDSVYFNPSEDKYNY"
     gene            5145..>5628
                     /locus_tag="O0V33_002362"
     CDS             5145..>5628
                     /locus_tag="O0V33_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCX8976964.1"
                     /translation="MSVLLLIIFIGGCGNMKDEQKKEEQTNKTDSKEEQIKKSFAKTL
                     DMYPIKNLEDLYDKEGYRDGEFEKGDKGMWVLYSSIVSEFKGESLKSRGMILKLDRNK
                     RTAKGSYIIRELKEDKNHDVQKNEKKYPVKLVNNKIIPTEDVKNEDLKREIENFKLFS
                     Q"
BASE COUNT         2424 a          553 c         1074 g         1577 t
ORIGIN      
        1 aattatttat atgaaaagag aatataaatg aagtataaaa cagagagacg tgaaatgatg
       61 ggaaatataa aaagttttgc attgtacata agtatcttgc ttttaatagt tgttgtagca
      121 ggttgtggca aaagtgataa aacaaaagaa gattccaaag aagaacaaat taaaaagagc
      181 tttgcgaaaa cattagatat gtatccaatt aagaatctcg aggacctata tgataaagaa
      241 ggatatcgag atggcgaatt taaaaaggga gataagggga cgtggacttt actcacaagt
      301 ttttcaaaaa gtaacaaacc ggatgaaata gatgacgaag gcatggtttt atatctaaat
      361 agaaatacca aaaaggcaac aggttattat tttgtaaata aaatttatga tgatattagc
      421 aaaaatcaga atgagaaaaa ataccgtgtt gaacttaaaa ataataagat tgttcttttg
      481 gataatgtag aagacgaaaa acttaaacaa aaaattgaaa attttaaatt ttttagtcag
      541 tatgcggatt ttaaagattt aaaaaattat caagatggaa gtataacaac taatgaaaat
      601 attcctagtt atgaagcaga atacaaattg aataatagtg atgaaaatgt aaaaaaactt
      661 agagatattt atccaattac aacgaaaaag gctccaatat taaagttaca tatagatggt
      721 gatataaaag gaagttcagt tggatacaaa aaaatagaat ataaattttc aaaagttaaa
      781 gatcaagaga caacattaag agattattta aattttgggc cgtctgatga agatagctaa
      841 agtttattag atgtagaaat aattctaaaa cagagagacg tgaaacgatg gggtatttaa
      901 aaaggtttgc attgtacata agcgttatga ttttaatgtt tgcgatagca ggttgtggca
      961 aaggtaatga aacaaaagaa ggttcaaaag aaacacaaat caaaaagagc tttgcgaaaa
     1021 cgttagatat gtatccaatc aaaaatctcg aggatttata cgacaaagaa ggctatagag
     1081 atggtgaatt tgaaaaaggt gacaaaggga tgtggacgat atacacagat ttcgctaaaa
     1141 gtaataaacc gggtgaattg gatgatgaag gtatggtttt aaatctggat aggaatacta
     1201 gaaccgctaa aggccattat tttgttacta cattttaccg gaatggtaaa ctgccagatg
     1261 aaaagaatta taaaattgaa atgaaaaata ataagattat tttattagat gaagtaaaag
     1321 atgataagct caagcagaaa atagaaaatt ttaaattttt cggtcaatat gcaaatctta
     1381 aagaattgaa aaaatataat aatggtgatg tttcaattaa tgaaaatgtt cctagttatg
     1441 atgttgaatt caaaatgagc aataaagatg aaaatgttaa gcaattaaga agtcgttata
     1501 atatttcgac tgaaaaatca cctatattaa aaatgcatat tgatggtgac ctgaaaggca
     1561 gttccgtagg ttatagaaag ttagaaattg acttttcaaa acgtgaaaac agcaaattat
     1621 cagtcattga atttttaagt tataaaccag cgaaaaaata gtattgataa ggaatattag
     1681 ggtgtgaaaa aatgggacat ctaaaaaagc ttgcactgtt cataagtgct attattttga
     1741 gcatttttat aataagttgt aatagttcaa tcgatactgc ggaaaaagca aaagaagatt
     1801 caaaagaaga acaaattaaa aagagctttg cgaaaacgtt agatatgtat ccaatcaaaa
     1861 atctcgagga tttatacgac aaagaaggtt atcgagatga cgaatttaaa aaaggcgata
     1921 agggtacatg gactttactc acaagttttg ctaaaagtaa caaaccaggc gagatagatg
     1981 acgaaggtat ggttttattc cttaatagaa atacaaagaa ggcaacagga tattattata
     2041 cgagcaaagt tcatgatgaa tttaatgaaa aagatcaaca aaaaaatatc atgttgaatt
     2101 aaaaaataat aagatagtac ttttggataa agtagaagat ccaaatctca aaaataaaat
     2161 agaaaatttt aaattcttta gtcagtatgc tgattttaga gatcttaaat attataaaaa
     2221 tggaaatata tcaagtacag ataatgtgcc aagctacgat gcagaatata aaatgagtaa
     2281 tacggataaa aatgtaaaaa aacttagaga agtttatcct attacaacca aaaagtcgcc
     2341 agtattaaaa ttacatatag atggcgacat aaaaggaagt tcagttggtt ataaaaatat
     2401 agaatataat ttttcaaaag taaaagacca agaaacagct gtaagagatt ttgtgaattt
     2461 tggaccatct gatggaggag ctaaagttta ttaggtgtag aaataattct aaaacagaga
     2521 gatgtgaaaa gatgggatat ttaaaaagga ttggaatgtg cataagccta ttgattgtaa
     2581 ttatttttgt aacatcttgc ggtggtggta ataagatcac tggagattca aaagaaacac
     2641 aaatcaaaaa gagttttgcg aaaacgttag atatgtaccc aatcaaaaat ctcgaggacc
     2701 tatatgataa agaaggctat cgagatggcg aatttgaaaa aggtgacaaa gggatgtgga
     2761 cgatatatac agattttgct aaaagcaata aatcagacga attggatgat gaaggtatgg
     2821 ttttaaatct ggatagaaat actcgaacgg ctaagggata ttattttgtt aagaaatttt
     2881 atgaaaagga taaattttca gatagaaaaa attataaagt tgaaatgaaa aacaataaaa
     2941 ttattttatt agacaaggta aacgatccaa accttaaaga aagaatagaa aattttaagt
     3001 tttttggaca atatgcaaat tttaaggatt tggaaaatta caacaatggc gatgtgtcaa
     3061 taaattggaa tgttccaagt tatgacgtgg aatataaaat gagcaataaa gatgaaaatg
     3121 ttaagcaatt aagaagtcgt tataacattc ctactgataa agctccaatg ttaaaaatgc
     3181 atattgacgg ggacttaaaa ggaagttctg ttggatataa aaggttagaa atagactttt
     3241 caaaagaaga tagggatatt tcagtcattg attatttaag ttataagcca gcgaaaaaat
     3301 agtgttgata attaaatatt agggtgtgaa atgatgagat atttaaatag agttgtactg
     3361 tacataattg ttatggtttt gagtgttttt ataataggtt gtgataaatc aagcgatact
     3421 tcagaaaagc caaaagaaga ttcaaaagaa gcacaaatta aaaagagttt tgagaaaaca
     3481 ttagatatgt atccaattaa gaatctcgag gatttatacg acaaagaggg atctcgtgat
     3541 ggtgagttta aaaaaggcga taaagggatg tggacgatat atacagattt cgccaaaagt
     3601 aataaacaag gtggattgag taatgaaggt atggtcttat acttagatag aaatacacgg
     3661 acagcaaagg gacattattt tgttaagaca ttttatgaaa aggataaatt cccagataga
     3721 aaaaaatata aagttgaaat gaaaaacaat aaaattatct tattagacaa ggtagaagat
     3781 ccaaaactaa aaaagagaat agaaaacttt aaatttttcg gacaatatgc aaaccttaaa
     3841 gaattgaaaa attacaacaa tggtgatgtc tcaattaatg agaatgttcc aagttatgac
     3901 gcaaaattta aaatgagcaa taaagatgaa aatgttaagc aattaagaag tcgttataat
     3961 attcctactg ataaagcacc ggtattaaaa atgcatattg atggtaattt gaaaggaagt
     4021 tctgtgggtt ataaaaagtt ggaaattgac ttttcaaaag gtgaaaaaag cgatttgtca
     4081 gtaatagatt ctttgaattt ccagccggcg aaggtggatg aagatgatga atgatgagga
     4141 tggtgtgtaa caatgaagtc tataaaaagg attggattgt gcattagttt gttgatttta
     4201 atcatctttg ttacatcttg tgatggtgat aataagatta ctggagactc aaaagaagaa
     4261 caaatcaaaa agagctttgc gaaaacatta gaaatgtatc caatcaaaaa cctcgaagat
     4321 ttatacgaca aagaaggata tcgagacgga gaatttaaaa aaggtgataa agggacttgg
     4381 gttattagat ctgaaatgaa aatccaatta aaaggagaaa atctggaatc taggggagta
     4441 gttttagaaa ttaacagaaa tactagaacg gctaaaggga attatattgt tagagaagtt
     4501 gttgaagata gcgatggaat gacacacaat catacaaaaa ggtatcctgt aaaaatggaa
     4561 aataataaaa tcattccatt aaaacaaatc gttgatgaaa aagtaaaaaa agaaattgaa
     4621 gaatttaaat tctttgtaca atacgggaat ttcaaagaat tggaaaacta taaagacgga
     4681 gaagtgacat ataacccaga agcaccaata tactctgcac aatatcaatt gaaaaacagt
     4741 gattacaatg tagaacaatt acgtaagcga tataatatcc cgacgcaaaa agcgcctaaa
     4801 ttattattga aagggtcagg taatctaaaa ggctcatcag ttggatataa aaatattgaa
     4861 tttacctttg ttgaaaataa gggagaaaat atttacttca cagatagtgt ctactttaat
     4921 ccaagcgagg ataaatataa ttactaatat aaaaaataac gttagaacat ttaataataa
     4981 agttaaatga attacatgta ttaaattgaa taagttagtt ttgtttgata acataaaagt
     5041 gtaataatca ctattttatt aagttgtatg aaatattcaa atgtgtataa aaataaagca
     5101 gagagatgtg aaatgatgga gtatataaaa aaattgcttt gtacatgagt gtattacttt
     5161 taatcatttt tattggggga tgtggaaata tgaaagatga acagaaaaaa gaggaacaaa
     5221 caaataaaac agattcaaaa gaagaacaaa tcaaaaagag ttttgcgaaa acgttagata
     5281 tgtatccaat taagaatctc gaggatttat acgacaaaga aggctatcga gatggcgaat
     5341 ttgaaaaagg tgacaaaggg atgtgggttc tgtattcatc tattgtatca gaattcaaag
     5401 gcgagagttt aaaatcacga ggaatgatat taaagttaga tagaaataag agaactgcta
     5461 aaggaagtta tattataaga gaattgaaag aagataaaaa tcatgatgtt caaaaaaacg
     5521 aaaaaaagta tccagttaaa ttggtgaata ataaaataat tccaactgaa gatgtaaaaa
     5581 acgaagattt aaaaagagaa attgaaaact tcaaattatt ttctcaat
//
LOCUS       DAKSQE010000134         5561 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.134, whole genome shotgun sequence.
ACCESSION   DAKSQE010000134 DAKSQE010000000
VERSION     DAKSQE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.134"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            93..1064
                     /locus_tag="O0V33_002363"
     CDS             93..1064
                     /locus_tag="O0V33_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8976965.1"
                     /translation="MKYLLKGKILLLLLILLTIISLFIGVSELSIKDIFHLSDSQQNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSISGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            1051..2007
                     /locus_tag="O0V33_002364"
     CDS             1051..2007
                     /locus_tag="O0V33_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCX8976966.1"
                     /translation="MRNNNKKIMLLIAVTFLISMLYLFVGIDFDIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVTFSAVLLVIILVITIFLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            2004..2765
                     /locus_tag="O0V33_002365"
     CDS             2004..2765
                     /locus_tag="O0V33_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8976967.1"
                     /translation="MIQVGNLTKTINNQMILDDISIEIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFDEGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHEIVNDALDLLQLQDIRKRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVAMKNGQLVKSD
                     LKDNVIQSSVLSDLYDMDIQIEHIRNQRICLYFKD"
     gene            2880..3909
                     /locus_tag="O0V33_002366"
                     /pseudo
     CDS             2880..3909
                     /locus_tag="O0V33_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
     gene            complement(4226..4540)
                     /locus_tag="O0V33_002367"
     CDS             complement(4226..4540)
                     /locus_tag="O0V33_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976968.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(4558..5481)
                     /locus_tag="O0V33_002368"
     CDS             complement(4558..5481)
                     /locus_tag="O0V33_002368"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCX8976969.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNVEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLVKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
BASE COUNT         1921 a          805 c          961 g         1874 t
ORIGIN      
        1 ctattgaaat gagtgacaaa aaacgatacc attaatgaga aatgttatca atgataataa
       61 ttatcattaa attaaaggga gaaaattttg taatgaagta tttattaaag ggaaaaattt
      121 tgcttctatt acttatattg ctaacaatta tttcgttgtt tataggtgtc agtgaactgt
      181 ctattaaaga tatctttcat ttgtcagact cacagcaaaa tatattgttc tcaagccgaa
      241 taccgagaac gatgagtatt ttaattgctg gaagttcatt agctttagct ggcctaatca
      301 tgcaacaaat gatgcaaaat aagtttgtta gtccaactac agctgggacg atggagtggg
      361 caaaattagg aatcttaatt gccctattat tctttccaac tggtcatatt ttattaaaac
      421 tagtatttgc tgttatttgc agcatcagcg gaacattttt atttgttaaa attattgatt
      481 ttatcaaagt gaaagatgtc atctttgtac cactattagg gattatgatg ggtggcattg
      541 tcgctagttt tacaacgttt atctcattac gtacaaacgc agttcaaagc attggtaact
      601 ggcttaacgg gaactttgcc attatcacaa gtggacgtta tgaaatcttg tatttaagta
      661 ttcctctttt agcattgaca tatctttttg caaatcattt cacaattgta ggtatgggta
      721 aagactttac caataattta ggtttgagtt atgaaaaatt aatcaatatt gctctattta
      781 ttactgccac aattacggca ttagtcgttg tgacagttgg aactttaccc ttcttaggtt
      841 tagttattcc aaatattatt tcgatttata gaggcgatca tttaaaaaat gcaattcctc
      901 atacgatgat gttaggtgcc atctttgtat tgttttctga tatagttggc agaattgtag
      961 tttatccata tgaaattaat attggtttaa caataggtgt atttggaaca atcattttcc
     1021 ttatcttact tatgaaaggt aggaaaaatt atgcgcaaca ataataaaaa aataatgctt
     1081 ttaattgcag tcacgttctt aattagtatg ctgtacttat ttgttggtat tgattttgat
     1141 atatttgaat atcaattttc aagtcgttta agaaagttca tattaattat tttagtaggt
     1201 gctgccattg caacatccgt tgttattttc caagcgatta caaataatcg tttattgaca
     1261 ccatcaataa tgggattaga tgcagtttat ttatttatca aagtattgcc cgtcttttta
     1321 tttggaattc aatcggtatg ggttactaat gtatatttga actttatatt aacacttata
     1381 acgatggtgt tattcgcact aatcctattc caaggtatct ttaaaattgg acatttttca
     1441 atttacttta tcttacttat aggtgtcctt ttaggaacat tttttagaag tattacaggt
     1501 tttattcaat tgattatgga tcctgagtca tttttagcaa tacaaagtag tatgtttgct
     1561 aattttaatg cttctaattc gaatttagtt actttttcag cagtattatt agtaatcata
     1621 ttagtcatta caatttttct attgccatat ttagatgtat tgcttttagg tcgtgctgaa
     1681 gcaattaatc tggggatatc gtatgaaaaa ttaacgcgga ttctacttgt aatagtttct
     1741 gtcttagtct ctgtgtcaac tgcattagta ggaccaatta catttttagg cttattgact
     1801 gtaaatctag cgcatgaact aatgaagacg tatgaacata aatatatatt aatcgcgaca
     1861 atttgcttga gttggattag tttatttagt gcgcaatggg tagttgaaaa tgtgtttgag
     1921 gctacgacag aaatgagtat acttattgat ttaattggtg gaagttattt catttatcta
     1981 ttagttagaa ggagaaatgc gcaatgattc aagttgggaa tttaactaaa actataaata
     2041 atcaaatgat attggacgat attagcatag aaattgaaaa aggtaagttg acttctttaa
     2101 ttggacctaa tggagcaggg aagagtacgt tactttcagc gatatgtcgc ttaattcggt
     2161 ttgatgaagg ggaagtaaaa atagatggac agcttatgtc tgattataaa aacaatgatt
     2221 tgtcgaaaaa gatatcgatt ttaaagcaaa cgaaccatac agaaatgaac attactgtag
     2281 aacaattagt taatttcgga cgatttccat attctaaagg acgtttaacg aaagaagacc
     2341 atgaaattgt taatgatgct ttagatttgt tgcaattaca agatattaga aagcgtaata
     2401 taaaatcatt gtctggtgga caacgtcaac gtgcctatat agcgatgacc attgcacaag
     2461 atactgaata tattctttta gatgaaccgt taaataattt agatatgaag catgctgttc
     2521 aaattatgca aacgttaaaa atgttagcgc ataaaatgaa taaagcgatt gtcattgtgt
     2581 tacatgatat taactttgcg tcctgttatt cagatcagat tgtagcaatg aaaaacggac
     2641 aactagttaa atcagatttg aaagataatg tcattcaaag tagtgtttta agtgatttat
     2701 atgacatgga tattcaaatt gaacatataa gaaatcaaag gatttgttta tattttaagg
     2761 attgataatt tggagacact ttaaaggggt gatgcgccaa ttaaagaagg gttaacaagt
     2821 atttattcgt atttcacatc aagcacacag attaagccaa aagaggagaa tattatatta
     2881 tgaagaaaac agtcttatat ttagtagtag cagtaatgtt tttattagcg gcatgcggta
     2941 acaattctga taaagaacaa tcaaaatcag aaactaaagg ttctaaagat acagtaaaaa
     3001 ttgaaaacaa ctataaaatg cgtggcgaga aaaaagatgg tagcgatgct aaaaaagtta
     3061 aagaaactgt tgaagtacca aagaacccta aaaatgcagt tgtattagat tatggcgcat
     3121 tagatgtaat gaaagaaatg ggcttatcag acaaagtaaa agcattacct aaaggtgaag
     3181 gcggcaagtc attaccgaat ttcttagagt catttaaaga tgacaagtat acaaatgttg
     3241 gtaacttgaa agaagttaat ttcgataaaa ttgcagcaac aaaaccggaa gtaatcttta
     3301 tctctggccg tacagcaaat caaaagaatt tagatgaatt taaaaaagct gcaccaaaag
     3361 caaaaattgt ttacgttggt gcagatgaaa agaatttaat tagttcaatg aaacaaaaca
     3421 ctgaaaatat tggtaaaatt tacgataaag aagataaagc taaagaatta aataaagatt
     3481 tagataacaa aattgcttca atgaaagata aaactaagaa gttcaacaaa tcagttatgt
     3541 atttattagt taatgaaggt gaattatcaa catttggacc taaaggtcgt ttcggtggat
     3601 tagtttacga tacattaggt ttcaacgcgg ttgataaaaa acgtaagcaa tagtaaccac
     3661 ggacaaaatg tctctaacga atacattaat aaagaaaatc cagatgttat tttagcaatg
     3721 gatagaggac aggctgtaag tggtaaatca actgcgaaac aagcattaaa taatcctgta
     3781 ttaaaaaatg ttaaagcaat taaagaagac aaagtttata atttagaccc taaattatgg
     3841 tactttgcag ctggatcaac tacaactaca attaaacaaa ttgatgaact tgaaaaagtt
     3901 gtaaaataat tttaaaagag gggaacaatg gttaaaggtc ttaatcattg ttcccctctt
     3961 tgctttaaaa aagggaatct gggacgtcaa tcaatgtcct agactctaaa atgttctgtt
     4021 gtcagtcgtt ggttgaatga acatatactt gtaacaagct catttcaata cctgtgggct
     4081 ccaaacatag agaaattgga tttccaattt ctacagacaa tgtaagttgc cgggctccaa
     4141 acatagagaa tatcaaaaag aaattctaca gaagtggtgc tttatcatgt ctatcccact
     4201 ccctataatt ttttggctat gttgtttaaa tttcgaaata aatatgatag tgatatttgc
     4261 agcgattgtt aaatcgagat tggcaattag gacaacgctc aaccatcata tattcattga
     4321 ttgttaattc atgtttgcat acaccgcata agattgcttt ttcgttaaat gaaggctcag
     4381 accaacgctt aatggcgtgc ttttcaaact cattatggca cttatagcat ggatagtatt
     4441 tattacaaca tttaaattta attgcaataa tatcttcttc ggtaaaataa tggcgacagc
     4501 gtgtttcagt atcgattaat gaaccataaa ctttaggcat agacaaagct ccttaactta
     4561 cgattccttt ggatgttcac caataatgcg aacttcacga tttaattcaa tgccaaattt
     4621 ttctttgacg gtcttttgta cataatgaat aaggttttca taatctgtag cagttccatt
     4681 gtctacattt accataaaac cagcgtgttt agttgagact tcaacgccgc caatacggtg
     4741 accttgcaaa ttagaatctt gtatcaattt acctgcaaaa tgaccaggcg gtctctggaa
     4801 tacactacca catgaagggt actctaaagg ttgtttagat tctctacgtt ctgttaaatc
     4861 atccatttta gcttgtattt cagtcatttt accaggagct aaagtaaatg cagcttctaa
     4921 tacaactaag tgttcttttt gaataatgct attacgataa tctaattcta attcttttgt
     4981 tgtaagttta actaacgagc cttgttcgtt tacgcaaagc gcatagtcta tacaatcttt
     5041 aacttcgcca ccataagcgc cagcattcat atacacagca ccaccaattg aacctggaat
     5101 accacatgca aattcaaggc cagtaagtgc gtaatcacga gcaacacgtg agacatcaat
     5161 aattgcagcg ccgctaccgg ctataattgc atcatcagat acttcgatat gatctagtga
     5221 taataaacta attacaatgc cacgaatacc accttcacgg ataataatat ttgagccatt
     5281 gcctaaatat gtaacaggaa tctcattttg ataggcatat ttaacaactg cttgtacttc
     5341 tacattttta gtaggggtaa tataaaagtc ggcattacca cctgtcttag tataagtgta
     5401 tcgttttaaa ggttcatcaa ctttaatttt ttcatttggg ataagttgtt gtaaagcttg
     5461 atagatgtct ttatttatca cttctcagta catcctttct catgtcttta atatcatata
     5521 gtattatacc aattttaaaa ttcatttgcg aaaattgaaa a
//
LOCUS       DAKSQE010000135         5554 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.135, whole genome shotgun sequence.
ACCESSION   DAKSQE010000135 DAKSQE010000000
VERSION     DAKSQE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.135"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            237..701
                     /gene="accB"
                     /locus_tag="O0V33_002369"
     CDS             237..701
                     /gene="accB"
                     /locus_tag="O0V33_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCX8976970.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPFEAAAMPMPQVQSTDNNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            701..2056
                     /gene="accC"
                     /locus_tag="O0V33_002370"
     CDS             701..2056
                     /gene="accC"
                     /locus_tag="O0V33_002370"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCX8976971.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            2071..2433
                     /locus_tag="O0V33_002371"
     CDS             2071..2433
                     /locus_tag="O0V33_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCX8976972.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            2493..2882
                     /gene="nusB"
                     /locus_tag="O0V33_002372"
     CDS             2493..2882
                     /gene="nusB"
                     /locus_tag="O0V33_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCX8976973.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKDHEPVLDETISPNLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFINGVLSNIKK"
     gene            2899..4236
                     /locus_tag="O0V33_002373"
     CDS             2899..4236
                     /locus_tag="O0V33_002373"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCX8976974.1"
                     /translation="MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHL
                     YFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLD
                     GIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSR
                     FPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVV
                     RAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFT
                     LTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQR
                     HRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNL
                     SPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            4229..4459
                     /locus_tag="O0V33_002374"
     CDS             4229..4459
                     /locus_tag="O0V33_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCX8976975.1"
                     /translation="MTKETQSFEEMMQELERIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            4437..5318
                     /locus_tag="O0V33_002375"
     CDS             4437..5318
                     /locus_tag="O0V33_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCX8976976.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMNTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDATKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELMQIDEQF
                     NTKHLLEIVDLFYSRDH"
BASE COUNT         2119 a          771 c         1044 g         1620 t
ORIGIN      
        1 agctaataaa aataacattg atatgtttaa ctgaatgcat aaaattttta atcaagagtt
       61 aagatgagtc aatgttaagt aataaatcca caagtgtttt taatttgaaa tgtggtttta
      121 ttataacttt gggatagacc tataatgtcg cctgcgatat gaacatgctt gaattgaaga
      181 gttgtctcaa gtaaaataga cgggtagatg aaaacaaact gaaggagtca gtaataatga
      241 actttaaaga aatcaaagaa ttaattgaaa ttctggataa atcaacttta acggaaatca
      301 atattgaaga tactaaaggc aaagtgacgc ttaagaaaga aaaagaaact gagattatca
      361 cgccacaaat ctcacaaatg ccatttgaag ctgcggcaat gcctatgcct caagtacaat
      421 caactgataa caataaaact gaagctccaa agccaacttc agataatcac aaaacaatta
      481 atgcacctat ggtaggtaca ttttacaaat cgccatctcc agacgaagaa gcctatgtgc
      541 aagttgggga cactgtttca aatgaaacaa cagtgtgtat tttagaagca atgaaactat
      601 ttaatgaaat tcaagcagaa atttcaggtg aaattgttga gatcttagta gaagacggac
      661 aaatggtaga gtatggccaa ccgttattta aggtgaaata atgaaaaagg ttttaattgc
      721 aaaccgcggt gaaatcgcag ttaggattat tcgcgcttgt cgtgatttag gcatccaaac
      781 tgttgcaatc tattctgaag gggataaaga tgcgctacat actcaaattg ctgatgaagc
      841 atattgcgta ggtcccactt tgtctaaaga ttcatattta aatattccga acatcttatc
      901 tattgcaact tctacaggtt gtgatggcgt tcatccgggt tatggctttt tagctgaaaa
      961 tgctgacttt gcagaattat gcgaagcatg ccaattgaag ttcattggac caagttatca
     1021 atctatccaa aaaatgggta tcaaagatgt tgctaaggca gaaatgatca aagccaatgt
     1081 tccagttgtt cctggtagtg acggtttaat gaaagacgtc tcagaagcta agaaaatcgc
     1141 taaaaaaatt ggttatccgg tcatcattaa agctactgct ggtggtggcg gaaaaggtat
     1201 ccgtgttgct cgtgatgaaa aagaacttga aactggcttc cgaatgacag aacaagaagc
     1261 acaaactgca tttggtaatg gtggacttta tatggagaaa ttcatcgaaa acttccgcca
     1321 tattgaaatc caaattgttg gggacagcta tggtaatgta attcatttag gagaacgtga
     1381 ttgtacaatt caaagacgta tgcagaaatt agtggaagaa gcaccttccc caattttaga
     1441 tgatgaaaca cgtcgtgaaa tgggaaatgc agcagttcgt gcagcgaaag ctgtaaatta
     1501 tgaaaatgcg ggaacaattg agtttatata tgatttaaat gataataaat tttattttat
     1561 ggaaatgaat acacgcattc aagtagaaca tcctgtaact gaaatggtaa caggaattga
     1621 tttagttaaa ttacaattac aagttgctat gggtgacgtg ttaccgtata aacaagaaga
     1681 tattaaatta acaggacacg caattgaatt tagaattaat gctgaaaatc cttacaagaa
     1741 ctttatgcca tcaccaggta aaattgagca atatcttgca ccaggtggat atggtgttcg
     1801 aatagagtca gcatgttata ctaattatac gataccgcca tattatgatt cgatggtagc
     1861 gaaattaatc atacatgaac cgacacgaga tgaagcgatt atggctggca ttcgtgcatt
     1921 aagtgaattt gtggttcttg gtattgatac aactattcca ttccatatta aattattgaa
     1981 taacgatata tttagaagcg gtaaatttaa tacaaacttt ttagagcaaa atagcattat
     2041 gaatgatgaa ggttaatagg aggtcaatcc atggtcaaag taactgatta ttcaaattca
     2101 aaattaggta aagtagaaat agcgccagaa gtgctatctg ttattgcaag tatagctact
     2161 tcggaagtcg aaggcatcac tggccatttt gctgaattaa aagaaacaaa tttagaaaaa
     2221 gttagtcgta aaaatttaag ccgtgattta aaaatcgaga gtaaagaaga tggcatatat
     2281 atagatgtat attgtgcatt aaaacatggt gttaatattt caaaaactgc aaacaaaatt
     2341 caaacgtcaa tttttaattc aatttctaat atgacagcga tagaacctaa gcaaattaat
     2401 attcacatta cacaaatcgt tattgaaaag taatgtcata cctaattcag taattaaata
     2461 aagaaaaata caaacgtttg aaggagttaa aaatgagtcg taaagaatcc cgagtgcaag
     2521 cttttcaaac tttatttcaa ttagaaatga aggacagtga tttaacgata aatgaagcga
     2581 taagctttat taaagacgat aatccagatt tagacttcga atttattcat tggctagttt
     2641 ctggcgttaa agatcacgaa cctgtattag acgagacaat tagtcctaat ttaaaagatt
     2701 ggactattgc acgtttatta aaaacggatc gtattatttt aagaatggca acatatgaaa
     2761 tattacacag tgatacacct gctaaagtcg taatgaatga agcagttgaa ttaacaaaac
     2821 aattcagtga tgatgatcat tataaattta taaatggtgt attgagtaat ataaaaaaat
     2881 aaaattgagt gatgttatat gtcagattat ttaagtgttt cagctttaac gaaatatatt
     2941 aaatataaat ttgatcaaga cccacatctc caatctgtat tgattaaagg tgagctttca
     3001 aatttcaaaa agcattcaag tggtcattta tactttaatg tgaaagataa agaaagcgtt
     3061 ataagtgcca tgatgttcaa aggtagtgct tctaaattaa acttcgaacc taaagaagga
     3121 gatgaagtct tattagaagc acgtgtttct gtttttgaac gtcgtggaaa ctatcaaatt
     3181 tatgtaaata aaatgcaatt agatggtata gggaacttat atcaaaaatt agaagcatta
     3241 aagaaaaaat taactgaaga aggttgtttt gataaagcga ataagaaatc aatacctaag
     3301 tttcctaaaa aaattgcagt tttaacagcg agtacaggtg ccgcaattcg agatatccat
     3361 tcaacgataa atagtcgttt tccattagct gaacaaatac aaattagtac tttggttcaa
     3421 ggtgaaaaag caaaagacga cattattgaa aaaattgaat atgcagatag tttaggtgta
     3481 gataccatta ttgtaggtcg aggcggtgga tccatagaag atttatggaa tttcaacgaa
     3541 gaagctgtcg tccgtgcaat atataattgt aagacaccga ttatatcagc agtaggtcat
     3601 gaaacagact ttacattaag tgattttgct gcagacatca gagctgcgac tccaactcaa
     3661 gctgctgtta ttgcaacacc agaccaatat gaattgctgc aacaaataca gcaatatcag
     3721 tttacattga ctcgtttcat caagaaacat ttagaacaac aacgtaaaca tgttgaacat
     3781 ttgtcatcgt attacaagtt taaacaacca actttattgt atgatcaaca gatacaacgt
     3841 cgtgatgatt tagaaaagag actgaaacaa caaattcagg caacttttga acaacagaga
     3901 catcgattaa tgctgttgca gcaacgctat aatttaaaag cgttactaag tagtgttaat
     3961 caagaacaac aaaataatct tcaattgaca aaccaacttg ttaaattatt gaattctaag
     4021 atattaagtt ataaaaatga tttgaaaaat aaagtagaaa acttaaacaa tttaagtcca
     4081 actaatacaa tgttgcgtgg atatgcaatt gttaataaaa aagacgaagt cattacgagt
     4141 actaaagatt taacagaaaa tgatcaattg acgttaacaa tgaaagatgg cttagtagat
     4201 gcaaaagtta cgaaagtaag gtgtaataat gactaaagaa acgcaaagtt ttgaagaaat
     4261 gatgcaagaa ttagagcgaa ttgttcaaaa attagataat gaaacagtat ctttagagga
     4321 atcattagat ttataccaac gtggtatgaa actatcagca gcttgtgaca caactttaaa
     4381 aaatgccgaa aaaaaggtga atgacttaat aaaagaagaa gctgaggatg taaaaaatga
     4441 cgaatctacc gatgaataaa ttaatagatg aagtcaataa tgaattatcg gttgcgataa
     4501 ataaatcagt aatgaatact cagctagaag aaagtatgtt gtattcatta aatgctggag
     4561 gtaaacgcat ccgaccagtt ctgttattac tcactttaga ttcactaaat accgagtatg
     4621 agttaggtat gaagagcgca attgcactag aaatgattca tacatattca cttattcatg
     4681 atgacctacc agcgatggat aatgatgatt atcgacgagg gaaattaacg aatcataaag
     4741 tatatggtga gtggacggcg atattagcag gtgatgcttt attaactaaa gcatttgaac
     4801 ttatttcaag tgatgataga ttaactgatg aagtaaaaat aaaagttcta caacggctgt
     4861 caatagcaag tggtcatgtt ggaatggtcg gcggtcaaat gttagatatg caaagcgaag
     4921 gccaaccaat tgatcttgaa actttggaaa tgatacacaa aacaaaaaca ggagcactat
     4981 taacttttgc ggttatgagt gcagcagata tcgctaatgt cgatgatgca actaaagaac
     5041 atttagaaag ttatagttat catttaggta tgatgtttca gattaaagat gatttattag
     5101 actgctatgg tgatgaagcg aagttaggta aaaaagtggg cagcgatctt gaaaataata
     5161 aaagtacgta cgtgagttta ttaggaaaag atggcgcaga agataaattg acttatcata
     5221 gagacgcagc agtggatgaa ctaatgcaaa ttgatgaaca attcaataca aaacacttat
     5281 tagaaatcgt tgatttattt tatagcaggg atcactaaaa gcaaatatat cgattagcaa
     5341 caattgttaa gtttgaaatg tctcaaaagg catattcaac tgacgttgtt tgttaatcga
     5401 tttttctcat gttgaaactt aaaattatgt taaagacttt catctaaaaa tagaattttc
     5461 aatttctata cgacaatatt cgcatctgtg atatcaatgt gattttaatt aaatatgttc
     5521 aaacaattga cgcttattaa atctaataat tagc
//
LOCUS       DAKSQE010000136         5411 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.136, whole genome shotgun sequence.
ACCESSION   DAKSQE010000136 DAKSQE010000000
VERSION     DAKSQE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.136"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(334..682)
                     /locus_tag="O0V33_002376"
                     /pseudo
     CDS             complement(334..682)
                     /locus_tag="O0V33_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633422.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
     gene            complement(723..1109)
                     /locus_tag="O0V33_002377"
                     /pseudo
     CDS             complement(723..1109)
                     /locus_tag="O0V33_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1185..2156)
                     /locus_tag="O0V33_002378"
     CDS             complement(1185..2156)
                     /locus_tag="O0V33_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCX8976977.1"
                     /translation="MTETKAIDTHAHLWNEDYLDKLGKLGSQGTEVAKGINQSASKED
                     LEKRFKMMDDAGVDLQIISATPQSPQWGTKEEAHQSAQEINDLYERLVQQYPDRFLAY
                     GAVSLPYVDQAIEEAQALLKKDEFVGIAIPTIVKDKVSVADKQFEPFFAAMNELNATI
                     YIHPTGCGAQSPLVNDYQLEWVIGAPLESTFITLQLIKNEIPQKYPNIKFHISHLGGA
                     LPFFMTRIKDNYEDWNAFNIDPYEVLNRQFWYDTANFHEPSLINSIETFGKDKFMMGS
                     DFPYFQDEKYTRGVDYIKNSNIDPDMINGILRGNAIEFYQIEENHNH"
     gene            complement(2209..2580)
                     /locus_tag="O0V33_002379"
     CDS             complement(2209..2580)
                     /locus_tag="O0V33_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017638058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCX8976978.1"
                     /translation="MLLRHVINTYVGSEIFKSSLPKVQNSDEMAQQFEQGFGLSRRSM
                     RLAGAFEMIGSLLLFTSIFGKLGKKLVVVGTIMINIVMGTAIYNHYKAGHGYKGAQAA
                     SKFFFLNVLSLLEVLSLTRNK"
     gene            complement(2600..3991)
                     /locus_tag="O0V33_002380"
     CDS             complement(2600..3991)
                     /locus_tag="O0V33_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCX8976979.1"
                     /translation="MRNVYAFNIDLKQRNRVIAVVMIGAFVGVLNQTLMTTILPEIMK
                     DFTVSSSTAQWLTTIFMLVNGIMIPITAFLIERFTLRSLFFNATCFLIIGSFICMLGI
                     NFPMLLVGRSIQALGAGILMPLTQTLLFIMFPPEKRGMAMGMFGLVIGFAPAIGPTAA
                     GWFVNIFDWRYLFLVVLLIGMVDFVFGYLSLPNITELSRPNLDKLSVILSTVSFGGLL
                     FGFSTAGNLGWSHPMVYITIIAAIVILTVFIFRQLKLESPLLEFRVFKYNDFSVAMIL
                     IVLMFMLFIGNLTILPIYMQTMMKWSPLESGLILLPGGLIMGLLSPVTGKLFDKIGGR
                     ILSIMGMLTIMIGALLMAQFSQNTTQLYVIISFSVTMLGNAMIMTPMTTQALNALPRQ
                     YIAHGTAMNNTIRQVSAAIGTGILVTLMTGLGKTSSLSGSQGLIHGLDITFYVVALIA
                     FIGTIIAFFIRKH"
     gene            4105..4308
                     /locus_tag="O0V33_002381"
     CDS             4105..4308
                     /locus_tag="O0V33_002381"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976980.1"
                     /translation="MKNAIMKGVVYINFEGLEQYLNGDNTRYNTLSLDALNYYLKQYV
                     ITIPFKNMDVQNGMKVSAEVKDI"
     gene            4318..4659
                     /locus_tag="O0V33_002382"
     CDS             4318..4659
                     /locus_tag="O0V33_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HCX8976981.1"
                     /translation="MNHQHGYFYYEMNNFFNVYFEYKGFAVNIVSSTIHTHGGGKCLK
                     GSQVSLIIPIDGVDYVAGAGFGDLPFSAMPIVPKDNSPIIHDMNGDFHAIYVNDYLFY
                     VRKMEKDNDNN"
     gene            4958..>5411
                     /locus_tag="O0V33_002383"
     CDS             4958..>5411
                     /locus_tag="O0V33_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCX8976982.1"
                     /translation="MEKNQRKKRSDAEYNQQIILTTMEDLLEQGEDISAKKMSDIAKI
                     SGVGVGTLYRHFESKTLLCQAIMDKKVDQMFIEIEDVLAENTQWPVRDKINIILTKYL
                     DLKEANFTTLNFIEKSSSHSSSVINIPFFERLKNLLIQQFENVHSIADL"
BASE COUNT         1854 a          875 c          861 g         1821 t
ORIGIN      
        1 agaagctagg aaaataaagc cgtcgattcc tgaaatgggt gaaaatggca ttgcaattag
       61 agatgaaatt gttttgacaa aatatttgta atatataaat atattcagct ttaaatcacc
      121 ctagtcctta ccaaaaagac ttttacacaa acaaaaaagg aggaaactta aattcctcct
      181 ttcccttatt acacctataa ttctatttta acgtcttcgt ccagttgggc ttcaaattca
      241 tctagaagtg ctcttgcttc tgcttaattt gaatcatata aggtgttttt tgttattcct
      301 aaaatttggc atagctaaat aaatctgtat tttctatttg tcttttttgg catttatgat
      361 aacccaagta tcttttggaa cggtaactgt agttgttgat tgaatttatt cgttttcact
      421 ttcatcgaag cttagtaatg atttatataa atctgatttc caaacccaaa cttctttttg
      481 ggtttgctgt gttgccttat tagtattctt cacttcatat ttatcctgat gcttaacgga
      541 ctcagatata ttattttttt cattttaggc ttgaagtgag ttgtagtttt ttatccttag
      601 gattatctct atagaccatg acatagttat ttgtattttt tcctatttca ttgattatta
      661 atatattagc tggattattt tggctctatg tcaaattgga ctgatgcatt caataaagga
      721 agttaagcaa ctaaacattg cttaacttcc tttttacttt ttggagcgta aagttttgaa
      781 cataataata tacgattgcg caaatgattg taatttccat aaccaaaaga cgtgcgttta
      841 attaatttta ttttgttatt tataccttct aaaggaccat ttgataaatt gtaataatca
      901 aatgtatttt ttataaagta tgcatgtttg ttaagtgtct taattgcccg ttggacaaat
      961 gaatgcgctt gagataatgg catatgtttt aattcgtgaa taaagccttc ataatcatta
     1021 tgtttaagtt tgaatcgtag ttgattgata tattggtagg cgtcatttag tgagtcatct
     1081 tgatctaata aatacttaat gatacctttt tatatagaaa gtaaagtagc gttattctaa
     1141 catatttagt ttataccata atataatgcc tctagaataa aacgctagtg attatggttt
     1201 tcttcaattt gataaaattc aattgcattg cctcttaata taccattaat catgtcaggg
     1261 tcaatattac tgtttttaat atagtctacg ccgcgtgtat atttttcatc ttggaagtaa
     1321 gggaagtctg accccatcat aaatttatcc ttaccaaatg tctcaataga gttaatcaat
     1381 gaaggttcgt ggaagtttgc tgtatcatac caaaattgtc gattcaatac ttcataagga
     1441 tcaatattaa aggcattcca atcttcataa ttgtctttaa tacgagtcat gaagaatggt
     1501 aaagcgccac ctaaatgtga aatatggaat ttaatatttg gatatttttg tggaatttca
     1561 tttttaatca attgtaatgt aataaaggta gactctaatg gtgcaccaat aacccattct
     1621 aattgatagt cattgactaa cggactttga gcgccacaac ctgttggatg aatataaatt
     1681 gtagcattga gctcattcat cgctgcaaag aaaggttcaa attgtttatc agcaacagag
     1741 accttatctt ttactatagt tggaatggca atgcccacaa attcatcttt tttcaaaagg
     1801 gcttgggctt cttcaatagc ttgatcgaca taaggtagtg agacagcacc atatgccaag
     1861 aaacgatctg gatattgttg aactaatctt tcatataagt cattaatttc ttgagcactt
     1921 tggtgagctt cttctttagt accccattgt ggagattgtg gtgtcgcaga aataatttgt
     1981 aaatctacgc cagcatcgtc catcattttg aagcgtttct ctaaatcttc tttagacgca
     2041 gattgattaa tccctttagc aacttcagta ccttgactac ctaatttgcc aagtttgtct
     2101 aaatagtctt cattccataa atgtgcgtgt gtatcaatcg cttttgtttc agtcatatta
     2161 tcccctccat taaaaattgt cattcataat ttgcttattg ccaatagctt atttattacg
     2221 agttaagctt aacacttcta gtaagcttaa tacatttaaa aagaaaaatt tagaagcagc
     2281 ttgtgcacct ttataaccat gcccagcttt ataatggtta taaatagctg tacccataac
     2341 aatattaatc ataatcgtac caacaactac taattttttc cctaatttac cgaaaataga
     2401 agtaaataaa agaagtgaac caatcatttc gaaagcgcca gctaatctca ttgatctacg
     2461 tgataaacca aagccttgtt cgaattgttg agccatttca tcactatttt gtacttttgg
     2521 taaactgctt ttaaaaattt cactacctac atatgtgtta atgacgtgtc ttaataacat
     2581 tttaaaatcc tccaaataat taatgtttgc gaataaagaa tgcaatgata gtacctataa
     2641 aggcaatcaa tgcgacgaca tagaatgtaa tatctagacc atgaataagt ccttgactac
     2701 cacttaatga agaggttttt cctaatcctg tcatcaatgt gaccaagata ccagtaccta
     2761 ttgcagctga tacttgtcta atcgtattat tcatggctgt accatgtgca atgtattgac
     2821 gtggcaaagc atttaaagct tgagttgtca ttggcgtcat gatcatagca ttacctaaca
     2881 tggtaactga aaatgaaata atcacatata gttgtgttgt attttgacta aattgagcca
     2941 ttaataatgc gccaatcata atcgttagca tccccataat actcaagata cgcccaccaa
     3001 ttttatcgaa taatttacca gtgacaggcg ataacagtcc cataattaac ccgccaggta
     3061 ataaaataag tcctgactct aacggggacc atttcatcat cgtttgcata taaattggta
     3121 agattgttaa attccctata aataacatga acattagtac aattaaaatc attgccactg
     3181 aaaagtcgtt atatttaaat actctaaact ctaacaatgg cgattccagt tttaattgtc
     3241 ggaaaataaa taccgttaat ataactatgg cagcaataat agttatataa accattggat
     3301 gtgaccaacc taagtttcct gctgtactaa aaccaaatag taagccgcca aagctaaccg
     3361 tcgataaaat aacggatagt ttgtctaagt ttggtctact tagttctgtt atatttggca
     3421 acgatagata cccgaataca aaatcaacca tacctattag taatacgact aggaacagat
     3481 agcgccaatc gaaaatattg acgaaccaac cagctgcagt tggtccaatc gcgggtgcaa
     3541 aacctattac aagaccgaac attcccattg ccattccacg tttttcaggt ggaaacataa
     3601 tgaaaaggag tgtctgtgtg agtggcatca gtataccagc acctaaggct tgaatactac
     3661 gtcctactaa taacattgga aaattgattc ctagcataca aataaatgaa ccaattatta
     3721 aaaagcatgt tgcattaaaa aataaagaac gtaatgtaaa acgttcaatt aaaaaagctg
     3781 tgataggaat cataatccca ttgactagca taaaaattgt cgtgagccat tgtgctgtac
     3841 tacttgatac cgtaaaatct ttcataattt ctggaagtat cgtagtcatc aaagtttgat
     3901 tcagcacacc aacaaaagca ccaatcatta cgacagctat aacacggttc cgttgtttca
     3961 aatctatatt aaaggcatat acattcctca aattttgacg cctccctctc ttttaagtgg
     4021 aacctactat catttatttt atacggaacg ggttccgttt gtcaatttaa taactcaagg
     4081 gtatgataag aggtatctga taaaatgaaa aatgcaataa tgaagggggt agtttatata
     4141 aattttgaag gattagaaca atatttaaat ggtgataata caagatataa cacgcttagt
     4201 ttagatgcgc ttaattatta tttaaaacaa tatgtgataa ctatcccgtt caaaaatatg
     4261 gatgttcaaa atgggatgaa agtttctgca gaagtaaaag atatttaaaa taaagtagtg
     4321 aaccatcaac acggttactt ttattatgaa atgaataatt tctttaacgt gtattttgaa
     4381 tataaaggat ttgctgtaaa tatagtttct tcaactatcc atacccatgg tggtggaaaa
     4441 tgcttaaaag gttctcaagt gtctttaatc atacctattg atggcgttga ttatgttgca
     4501 ggtgctggct ttggagactt gccatttagt gccatgccaa ttgttccaaa agataatagt
     4561 ccaatcattc atgatatgaa cggtgatttc catgccattt acgtcaacga ttatttattt
     4621 tatgttcgaa aaatggagaa agataatgac aataactgag atcacactat gaagcggaat
     4681 taacacctcg tcatattcat gactttgttt acaatattga atataatgaa attaatccta
     4741 atgctatttt tgtgaaacgc tagttagtaa caatgattaa atctttttgg tcgtgctaca
     4801 atgttacaaa ataatataac tttaactaag caacaaaaga aagaataata tgatgtaaca
     4861 cctgagaatg acagacaatt tttagaagaa gtatttaatt tagatgttaa aattgataga
     4921 ttagaaagtt aaaaacaacg ttaaaaagag gaatataatg gaaaaaaatc aacgaaaaaa
     4981 acgcagtgat gccgaatata accaacagat tatcttaact acaatggaag atttactaga
     5041 acagggtgaa gatataagtg ctaagaaaat gtcagatatt gctaaaatat caggtgttgg
     5101 agttggtaca ttatatcgtc attttgaaag taaaacattg ttatgtcagg ctattatgga
     5161 taaaaaagtc gatcaaatgt ttatagaaat agaagatgtt ttagcggaaa atacacagtg
     5221 gcccgtgcga gataaaatca atattatatt aacaaagtat ctagatttaa aagaagcaaa
     5281 tttcacaaca ttaaatttta tagaaaaatc cagttcacac tctagttctg taattaatat
     5341 tccattcttt gagcgactaa aaaacttatt aattcaacaa ttcgaaaatg ttcattccat
     5401 agcagacttg g
//
LOCUS       DAKSQE010000137         5408 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.137, whole genome shotgun sequence.
ACCESSION   DAKSQE010000137 DAKSQE010000000
VERSION     DAKSQE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.137"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..256
                     /locus_tag="O0V33_002384"
     CDS             <1..256
                     /locus_tag="O0V33_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCX8976983.1"
                     /translation="NKGIGSHLLNYAEIIASDMSKRSISVITTNENIKALYFYQKNKY
                     RITDVIFNAVTEARKIKPSIPTIDKNGIEIRDEIVLKKCL"
     gene            complement(361..1347)
                     /locus_tag="O0V33_002385"
     CDS             complement(361..1347)
                     /locus_tag="O0V33_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCX8976984.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKLEL"
     gene            1656..1859
                     /locus_tag="O0V33_002386"
     CDS             1656..1859
                     /locus_tag="O0V33_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976985.1"
                     /translation="MEDLKQSLKGLGWYDLFFTVPMFLLFVYLPNYNFITIFINIVII
                     IFFSIGLILTTHIIIDIIKSNSK"
     gene            1908..2204
                     /locus_tag="O0V33_002387"
     CDS             1908..2204
                     /locus_tag="O0V33_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HCX8976986.1"
                     /translation="MATEKDVNDLFLNHVNSNAVKTRKMMGEYIVYYDGVVIGGLYDN
                     RLLVKATKSARHQLQDNTLISPYPGAKEMILIPHFVKVTNLTDLFELIKNDLQK"
     gene            complement(2267..3487)
                     /locus_tag="O0V33_002388"
     CDS             complement(2267..3487)
                     /locus_tag="O0V33_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCX8976987.1"
                     /translation="MTDLLKIKNFRLFFLAEIISAFGVGISTVEANWYLIDKTNDSQL
                     LGIMLALNVLSGFLASPIIGGLADKYNRRNIILITYLLQVILYLLIVIALLMIGFETY
                     LVIGFAIVNGIGWTTYMATSRSLVKQILKPDQYTDANSLLEISLQTGMFIAGGLSGIL
                     YEINGFTLIIAMTIMMFLISIFMLFRLHVDKPTHSEEESTNSLLQEYLLGWKFLKDNM
                     MIFIFGVISIIPMVFTMIFNISLPGYVYNVLKLSSVQFGFSDMLYGIGGLCAGLISAI
                     LSKKISTKALIFLLYFILVINSALFIWINSAFYLFIGSFILGYSISSIRIYMNTAIMN
                     TVSDKYVGRSFTIWTSISLLLQSFIAPFLGRWINEINDKFGFYIILILSLLIFVTLLL
                     VNKTDKIKYAHKEE"
     gene            complement(3480..4030)
                     /locus_tag="O0V33_002389"
                     /pseudo
     CDS             complement(3480..4030)
                     /locus_tag="O0V33_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
     gene            4143..5003
                     /locus_tag="O0V33_002390"
     CDS             4143..5003
                     /locus_tag="O0V33_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCX8976988.1"
                     /translation="MNLNILKSFLVTSETKNLTKASELLNYSQSTVSTHIEKLEKQLD
                     VKLFYRKKYGMELTEEGLAYVKYAKVILDSNSEYEREIKGLYNKKVNISINMQESQYL
                     YRYYNKISEWLAEHPYVNLKFKSAHSNFYIKEEIANFKSDISLITDEKIINSNLTAIP
                     ITEERLIFVTNKEMKDFKLNDIQNHTLLVTEKGCSYREQLEKILYKYSFSTKQMVEFL
                     GIESLKKHLKNSHGIALLPEFIVADELENKSLFQIDVDVDIPNLETTLIINPESSKQV
                     LESFVKDVFL"
BASE COUNT         1912 a          813 c          835 g         1848 t
ORIGIN      
        1 gaacaagggt attggtagtc atttacttaa ttatgctgaa ataattgcct cagatatgag
       61 taaaagaagt atcagtgtta ttactacaaa tgaaaatatt aaggcactct atttctatca
      121 aaaaaataaa tatagaatca cagacgttat ttttaatgct gtaacagaag ctaggaaaat
      181 aaagccgtcg attcctacaa ttgataaaaa tggcattgaa attagagatg aaattgtatt
      241 gaaaaaatgt ttataacgta taaatatacg ttgttttaaa tcaccctagt tcataccaaa
      301 aagcctttaa cacaaacaaa aaaggaggaa cattatattc ctccttgacc ttcattacat
      361 ctataattct agtttaacgt cttcgtccat ttgggcttca aattcatcga gtagtgctcg
      421 tgcttctgca attgattgtg tgttcatcaa ttgatgtcga agttcactag cgcctcttat
      481 gccacgcaca tagattttaa agaatctacg caagctcttg aattgtcgta tttcgtcttt
      541 ttcatatttg ttaaacaatg atagatgcaa tctcaacaga tctaatagtt ctttgcttgt
      601 gtgttcgcgt ggttcttttt caaaagcgaa tggattatgg aaaatgcctc taccaatcat
      661 gacgccatca atgccatatt tttctgcaag ctcaagtcct gtttttctat cgggaatatc
      721 accgttaatt gttaacaatg tatttggtgc aatttcgtca cgtaaatttt taatagcttc
      781 gattaattcc caatgtgcat ctactttact catttcttta cgtgtacgaa gatgaataga
      841 taaatttgca atgtcttgtt cgaagacatg cttcaaccaa tctttccatt catcgatttc
      901 atagtagcca aggcgtgttt taacacttac cggaagcccg cctgctttag ttgcttgaat
      961 aatttcggca gcgacgtcag gtcttaagat taagccggaa cccttaccct ttttagcaac
     1021 atttgctaca ggacatccca tatttaagtc aatgccttta aagcccattt tagctaattg
     1081 aatactcgtt tcacggaact gttctggctt atctccccat atgtgagcga ccatcggctg
     1141 ttcatcttca ctaaaagtta agcgtccgcg cacactatgt atgccttcag ggtgacaaaa
     1201 gctctcggta tttgtaaatt cagtgaaaaa cacatccggt ctagctgctt cacttacaac
     1261 gtgtcgaaag acgatatctg taacgtcttc cattggcgcc aaaataaaaa atggacgtgg
     1321 taattcactc caaaaatttt ccttcataat atatttatac cctctttata attagtatct
     1381 cgatttttta tgcatgatga tattaccaca aaagacgaac ttatacaaaa ggaattttag
     1441 ttgatacaac catttgaaag ggaagtctac gagtagtcta aaatgaatgt tgtggtaagt
     1501 tgatcagtat acagatcaag gattatcgta ttagatagtt cattattaat gatacactac
     1561 ttattaatat gattcagaat tttctttagc tacatataca gtaatgtgat ttatacgtta
     1621 tcttataaca aagacaaatt tataaaggtg atattatgga agatttaaag caatctttaa
     1681 aaggtttagg ttggtatgac ttatttttta cagtacctat gtttctacta ttcgtgtatc
     1741 tgccgaatta taattttata actatattta ttaacattgt tatcattatt ttcttttcca
     1801 taggtttgat tttaactacg catataatta tagatataat taagagcaac tcgaaatgat
     1861 tcattaacac gaatgtgatt aaacataaaa ctgaaggagc gattacaatg gcgactgaga
     1921 aagatgtaaa tgatttattt ttaaatcatg tgaattcaaa tgctgttaaa actagaaaga
     1981 tgatgggaga atatattgtt tattatgatg gtgtggttat aggtggtttg tatgataata
     2041 gattattggt caaggcgact aaaagtgcac gtcatcaatt gcaagataat acattaatat
     2101 cgccatatcc tggtgccaag gaaatgattt taattccaca ctttgtcaaa gtaacaaatc
     2161 tcactgattt atttgagctc ataaaaaatg atttgcaaaa gtgaagttcg gatggagaga
     2221 attaagcaca tcgacaaatg atcgcatgtt aaaagaagag tgacggtcac tcttctttat
     2281 gtgcatattt tattttgtct gttttgttaa cgagcagcag tgtaacaaat atgagtaagg
     2341 ataaaatgag tataatatag aaaccgaatt tatcattaat ttcattaatc catcttccta
     2401 aaaatggagc aatgaaactt tgcagtaaca atgaaattga cgtccatatc gtaaatgagc
     2461 gaccgacata tttatctgaa acagtgttca ttatagctgt attcatataa attctgattg
     2521 atgaaattga gtagcctagt ataaatgatc ctatgaataa gtaaaatgct gagtttatcc
     2581 aaataaatag tgctgaattt atgactaata tgaaatataa caaaaatatc aatgctttag
     2641 ttgagatttt tttcgaaaga atagctgaaa ttaaacctgc acataatcct ccaattccat
     2701 ataacatatc tgaaaaacca aattgtacag acgaaagttt taaaacatta tatacatatc
     2761 ctggtaatga tatgttaaag atcattgtaa acaccattgg tatgattgaa ataactccaa
     2821 atataaatat catcatgttg tcttttaaaa atttccatcc taataaatat tcttgcaata
     2881 agctatttgt tgattcttcc tctgaatgag ttggtttatc tacatgcaat ctaaataaca
     2941 taaaaatgct gattagaaac atcattatag tcatcgctat aattagagtg aatccattta
     3001 tttcatataa tattcctgat aatccacctg caataaacat acctgtttgc aaactaatct
     3061 ctaatagaga atttgcatct gtatattgat ctggttttaa aatctgttta actaaacttc
     3121 tagatgttgc catatacgta gtccaaccta tcccattaac aatcgcaaat ccaatcacta
     3181 aataggtctc aaagcctatc attaagagtg ctatgacaat gagtaaatat aatattacct
     3241 gaagaagata ggtgattaaa ataatatttc gtctattata tttatcggct agtccaccta
     3301 ttataggaga agctaagaaa ccggataata catttaaagc taacataata cctaatagtt
     3361 gggagtcgtt agttttgtcg attaaatacc aattagcctc aactgtacta attccaactc
     3421 cgaatgctga aattatttct gctaaaaaga acaatctgaa gtttttaatt ttcaacaaat
     3481 cagtcataaa tatcaccttt tttcaatgca agatttatat ttgcgagctc ttctataaat
     3541 tgagagtcgt ttaaaatatc gaatggttta ttgccttcgc cgatcaaaaa tctatagtta
     3601 gtaatattca taaactcaaa tattaatttg aattggttga ctaaaggttg tgctttggta
     3661 tgcggattgt cgccacctat gattaaaatc aaatactttt tttgtgacat gatatctttg
     3721 aagttgtcta tttgcgtatc tcgcaatgac tcggtccatc tatcaatgaa gagttttaaa
     3781 gacgcactca tggagtacca atagagggtg ttgaaaaaat aataatatct gattctaaaa
     3841 ctttgattaa aatttgttcg taatcatcat tatgaaagtt ggcacctttg ctatgacgat
     3901 tatctgtaac cttttcaatg ttactttgat ataaattcac aaagttgaca tttaaattct
     3961 tcaataggtt ctctactgcg atagctgaat tgccatcttt tctactactt ccaaataaac
     4021 aagtaatcat agtcataact cctttcgatt tacctttata ataatattta atatattatt
     4081 ataaaatcag aattcttaga atgcagaatt cgataaaagt gaattctaaa ggaggtacta
     4141 ctttgaactt aaacatttta aaatcatttt tagtaactag tgaaacaaag aaccttacta
     4201 aagcatcaga attacttaac tattcacagt caactgtatc tacacatatt gaaaaattag
     4261 aaaagcaatt agatgttaaa ttattttata gaaaaaaata tggtatggaa ctaacggaag
     4321 aaggcttagc atacgttaaa tacgctaaag tgattttaga tagtaatagc gaatacgaga
     4381 gagaaataaa aggactttac aataagaagg taaatataag tattaacatg caagaaagtc
     4441 agtatttgta tcgctactat aataagatta gtgaatggtt agctgaacac ccatatgtaa
     4501 acttgaagtt taaatccgca cattctaatt tctatattaa agaagaaatt gctaatttta
     4561 aatcggatat tagccttatc acagacgaaa agattattaa tagtaactta actgctattc
     4621 ctataactga agaacgttta atatttgtta ctaacaaaga gatgaaagat ttcaaattaa
     4681 acgatattca aaaccataca ttacttgtta ctgaaaaagg gtgtagttac agagaacagt
     4741 tagaaaagat tctctataaa tatagctttt caacaaaaca aatggttgaa tttctaggaa
     4801 ttgagtcatt gaaaaaacac ttaaaaaatt cacatgggat tgcactattg ccggagttta
     4861 ttgttgcaga tgaattagaa aataaaagtt tgtttcaaat agatgtcgat gtcgatattc
     4921 cgaatttaga aacaactttg ataattaatc ctgaatccag taagcaagta ctagaatctt
     4981 ttgtaaaaga tgtcttttta taaatattgg tgaaaacgtg tagttatggt gaaactcaaa
     5041 gataataatt taaatgagat gttaatgaaa aagcaattca atataaaaac aggtgattta
     5101 tatctttaat aaggattatt tctagttgaa atgtcaaatt gcgggcattc attttcaaaa
     5161 tagtaaagaa tttaattgat gaaaacctct gaatcatact agttagtatg gattgattaa
     5221 tattttaacc tgctaaatga gtcgcttatg tattttaaat agctgtcatc tagcaggttc
     5281 tttgttatta ataagttcag ataaattatt ttagtctgtt ttctaatgtt gataataata
     5341 actctaaatc tgtactttca ttgtgagata tatagtacat cataaatttt tatatttgga
     5401 ttgagact
//
LOCUS       DAKSQE010000138         5387 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.138, whole genome shotgun sequence.
ACCESSION   DAKSQE010000138 DAKSQE010000000
VERSION     DAKSQE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.138"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(99..1907)
                     /locus_tag="O0V33_002391"
     CDS             complement(99..1907)
                     /locus_tag="O0V33_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCX8976989.1"
                     /translation="MHFHTHLLYNTCEKYCLGAVFFYFWGVFLYNSLHKCKGFSFHVL
                     LSLTEILKGCLATMDKELWIKRANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRGK
                     FGLGEGRLNKFTIEKLALGLARYLNAQTNNPTIVIHYDIRHLSTEFAQIIANVLANHQ
                     IIVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYSSDGAQLSTDASE
                     LVSRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVVF
                     TSLHGTSVPIVPELLKSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELAN
                     KSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMIQ
                     SIVSSELTKSLARYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPFV
                     RDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGLEGKKKI
                     ESIMTHFRSNPPQEIQGLKVKAIEDYLTSEVYQLDKDTTSQIDSPKSNVIRVLFDEGF
                     IALRPSGTEPKIKLYVSLKCPDFDDVAQKINAMIFS"
     gene            2024..2416
                     /locus_tag="O0V33_002392"
     CDS             2024..2416
                     /locus_tag="O0V33_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HCX8976990.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEKMSLPLMWEFPGGKVEKNE
                     TEKDALIREIREEMKCDLIVGDKVITTEHEYDFGIVRLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
     gene            2418..5279
                     /locus_tag="O0V33_002393"
     CDS             2418..5279
                     /locus_tag="O0V33_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HCX8976991.1"
                     /translation="MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTI
                     IDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGEL
                     LKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
                     GDLVDSVKNEFDLLWQNSTQLTQQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKS
                     VEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVH
                     NEGILNRAKEEFKKVLPTKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDEK
                     EFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFEMFDYNIAYEIR
                     LQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGE
                     ILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLREGKINYIITV
                     DLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNYKTNYL
                     IPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLIL
                     KAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSNYYEFLVRYKKIDALLTENESKN
                     LVFFSRQIAPGLKRIDSIVLEELLKNELTYDELKNKMLNEVKDITEDDIDTSLRILDF
                     SFYNAGIEKIYGSPIIECNERMIRLSDAFTNALSNQTFKIFLEDLIELSKYNNEKYQK
                     GKNGLILYNKYSREDFSKIFNWSKNGSSVIMGYMIRSQEMPIFITYDKHEDISDSTKY
                     EDEFLSQDELKWFTKSNRTLKSKEVQKILSHRAKGIKMYIFVQKKDDDGIYFYYLGTA
                     GYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYITN"
BASE COUNT         2012 a          702 c          924 g         1749 t
ORIGIN      
        1 attagcccct tcaaaacgcg aaaacataag gattctagtt tcaaaagggc tgataagtat
       61 aaaatgaaat gtaatatttc gatgtttaaa atttttaatt agctaaaaat catcgcatta
      121 attttttgag ctacatcatc aaaatccgga cattttaacg acacatataa tttaatttta
      181 ggttcagtac cagaaggacg taaagcgata aatccttcgt caaataagac acgaataaca
      241 tttgacttag gagaatcaat ctgcgacgtt gtatctttat ccaactgata aacctcgcta
      301 gttaaataat cttcaattgc tttcactttc agtccttgaa tctcttgcgg tggatttgaa
      361 cggaaatgtg tcataattga ttcaattttt ttcttacctt ctaaaccttc tagcgtatgc
      421 gagaataatg tatcctcatg tctaccaaca gtttgataaa tctgttctaa ttcatctttc
      481 aatgttttgc catataattt taactcagaa gcgtatttta taatgagtgg cacaatttgt
      541 acggcatctt tatcacgtac aaaaggctct gatagaaaac cgtaactctc ttcaaatgcg
      601 aaaatcatat tttgatgatc gtccagttgt cttatttcct gagcaataaa tttaaagccc
      661 gtcagcacct ctttgtattc aacattatta tagcgtgcta atgactttgt taactcacta
      721 cttacaatag attgaatcat taagcggtga cgtaattgag acgtttgttg aatacgataa
      781 ttaagtaaca gcgcaccaat ttggttgcca ttaaaatatg tgatatgacc gtgtgcatca
      841 cgttcagcaa taccaagacg atccgcatca gggtcagttg atattaataa gtctgcatga
      901 cttttatttg caagttcgac tgcttgatca aaagcgcgat gatcttcagg attagcactt
      961 tgaactgagc taaaattcgg atcaggttta cattgtgctt ctacaagatt gaattgattg
     1021 aaattcaaag attttaataa ctcaggtaca atcggaacac ttgtaccatg caaactagtg
     1081 aacacaactt gtaaatcaga tttaggaata tagccaatca tattttgaat gtgtttcata
     1141 tagtcatcag ttacagattt cgggaaaggc ttgatataag atgtattttg ttttgaaata
     1201 ggtatatcaa tctgtaatgg atcgccaact tcttcgatat aacgacttac aagctcagat
     1261 gcatcggtcg atagttgcgc gccatcagaa ctatatactt taatgccgtt atagtctttc
     1321 ggattatgac ttgctgtaat cataatgcca gcagtagtat taagattgcg caccgcgaac
     1381 gataattccg gtgtcgtttt atatgtatca ggtaaataaa ctattatttg atggtttgct
     1441 aatacattag caatgatttg ggcgaattca gttgaaagat gtctaatatc ataatgaatg
     1501 actattgttg gattgtttgt ttgggcattt aaataacgcg ctaaacctaa tgccaatttt
     1561 tcaatagtaa acttattaag tcgaccttca ccaagaccga attttccgcg tatacccgca
     1621 gtaccaaacg ttaatttact ttcaaaacct tctcgctgtt caatgtcaga ttgttgctca
     1681 taaaaatgtt taactaaact atcattagct ctttttatcc ataactcttt atccattgtt
     1741 gctaaacatc ctttcaaaat ctcagttaga cttaataaaa catgaaaact aaagccctta
     1801 catttatgta atgaattata aagaaatacg ccccaaaagt aaaaaaacac agccccaaga
     1861 caatactttt cacaagtatt atataataga tgtgtatgaa aatgcatgga gtagagcgtt
     1921 catgtgaatg taaaatatta atttttaata ttgatttata tatagttttg taatttttga
     1981 tagaataaca attaagatga ttttaaattg aagggtgagt cttatgaaaa aagtaatcaa
     2041 tgtagtagga gctattattt tttctgataa caaaattctt tgtgcacaga gaagtgaaaa
     2101 aatgagtctg cctttaatgt gggaatttcc tggcggtaag gttgaaaaga atgaaactga
     2161 aaaagacgct ttgattagag aaattagaga agaaatgaaa tgtgatctaa ttgttggaga
     2221 caaagttata actacagaac atgaatatga ttttggaatt gttaggttaa caacatacaa
     2281 atgtacttta aacaaagagt taccaactct aactgaacat aagagtattg aatggttgtc
     2341 aataaacgaa cttgataaat taaattgggc cccagcggat ataccagccg ttaataaaat
     2401 tatgactgag ggataatatg agtagattac taaatgattt caatcaatct ttacataaag
     2461 ggtttataga caaacatatt tctcataaag ggaattatac accgaagctt ttagtaaata
     2521 ataaaaatga aaaagttctt tcaacaatta tagatgaact tcaaaaatgt gaaacgttct
     2581 atttttctgt ggcttttata acagaaagcg gtttagccag cttaaaggct caactattag
     2641 atttaagcaa taagggtgtt aaagggaaaa tattaacatc taattactta gggtttaata
     2701 gtcctaaaat gtatggagaa ttacttaaat taaaaaatgt agaggtaaga ttaaccgata
     2761 ttgctggatt ccatgccaaa ggatatattt ttgaacataa ggattatagt tctatggtaa
     2821 taggaagctc taatttaaca tcgaatgcgt taaaggttaa ttatgagcat aatgttttac
     2881 tgtctactat gaaaaatggg gatttggttg atagtgtaaa aaatgaattt gacttgttat
     2941 ggcaaaacag tacccaactg actcagcaat ggattaattc atataaagaa tcatttgagt
     3001 accgctcctt agaaaaattg gcggaggttg aacaaactca aatgctgcta gctgataagg
     3061 tgaaaaaatc agtggaaatt gtaccgaatt taatgcaagc agaagcatta aggtcattaa
     3121 aagctattag ggataaagcc aaagataagg cgttaataat atctgcaact ggtacaggta
     3181 aaacgatttt atgtgcatta gatgttagag aagtaaaccc taataaattt ttatttattg
     3241 ttcataatga gggaatttta aatagagcta aggaagaatt taaaaaagta ttacctacaa
     3301 aaaatgatag tgattttgga ttgttgacag gcaaacatag agatgtcgat gccaaatatt
     3361 tatttgcaac aattcaaaca ttatctagag atgataattt taagcagttt gatgaaaaag
     3421 aatttgatta tattgttttt gatgaagcac atcgttctgc tgcatctact tatcaacgag
     3481 tatttaatta cttcaaacct aagtttatgc tgggtatgac tgcaacacca gaaagatcag
     3541 atgaattaag tatatttgaa atgtttgatt ataatattgc ttatgaaata aggttacaag
     3601 cagcattaga aagtgatatt ttatgtccat ttcattattt tggtgtgact gattatgtac
     3661 atcaaggtat taaagaagat gatgtaacta aactgagata tttaacttct gatgaaagag
     3721 ttaattatat tattcaaaag acagattact atggatattc aggtgaaatt ttacaaggat
     3781 taatttttgt aagtagcaaa aaagaagctt atgatttagc ggataaacta agttcaaaag
     3841 ggattaaaag tgttgcttta acaggagacg atagtgttaa ttatcgccaa attgtaatag
     3901 aaaaattaag agaaggtaag attaactata ttataactgt agatctattt aacgaaggta
     3961 ttgatattcc agaagtcaat caagttgtaa tgctgagacc aactgagtct agtattatat
     4021 tcattcagca acttggtaga gggttaagaa aaagttcaaa taaagagtat gttacagtaa
     4081 ttgattttat tggtaattat aagacaaatt atttaattcc gattgcgctt tctggggatc
     4141 aatcgcaaaa taaagataat tataagaagt ttttaacgaa taacgattcg attaatggag
     4201 tatctacaat taattttgaa gaagttgcta aaaaacagat ttacaattca ttggatgcag
     4261 tatcattgaa tcaaaataaa ttaatattaa aagcttatga agaagttgaa aatagattgg
     4321 gacacatgcc gttactaatg gattttatac aacaacattc aatagatcca agcgttatat
     4381 tttctaaatt tagtaattat tacgaatttt tagtaaggta taaaaaaata gatgctttat
     4441 tgacggaaaa tgaatctaaa aatctggttt tcttttcaag acaaatagca cctggattga
     4501 aaagaatcga tagtatagtt ttagaagaac ttttaaaaaa tgaattaaca tatgatgaat
     4561 taaaaaataa aatgttgaac gaagttaagg atataacaga agatgatata gatacttcat
     4621 taagaatttt agatttttca ttttacaacg caggaattga aaaaatctat ggaagtccca
     4681 ttatagaatg taatgaaaga atgataagac taagcgatgc atttactaat gctctaagta
     4741 atcaaacatt taaaatattt ttagaagatc ttattgaatt atcaaagtat aacaatgaaa
     4801 aataccaaaa aggtaaaaat ggactgattt tatataataa atattcaaga gaagatttta
     4861 gtaaaatatt taattggagt aaaaatggtt cgtctgtaat catgggatac atgataagaa
     4921 gccaagaaat gccaatattt atcacttatg ataagcatga ggatataagt gatagcacaa
     4981 aatatgaaga tgaattttta agtcaagatg aattaaaatg gtttactaaa tctaatagaa
     5041 cgctaaaatc aaaagaagtt cagaaaattt tgtcccatag agcaaaagga attaaaatgt
     5101 atatttttgt acagaaaaaa gatgatgatg gtatatattt ttattattta ggaactgctg
     5161 gatatataga aggttcagag aagcaagata aaatgccgaa tggttcaaac gtagtaacta
     5221 tggatttagc actcgataaa gctgtcagag atgatatata tcgctatata acaaattaat
     5281 gtaaaccgaa aaatgaatgt tagtaaagat gtatttaaac atcaatttta acaagtaaag
     5341 acattgagtt tcagtgttat tattttaaat ataagcgcaa tttatct
//
LOCUS       DAKSQE010000139         5051 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.139, whole genome shotgun sequence.
ACCESSION   DAKSQE010000139 DAKSQE010000000
VERSION     DAKSQE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.139"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(27..908)
                     /locus_tag="O0V33_002394"
     CDS             complement(27..908)
                     /locus_tag="O0V33_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8976992.1"
                     /translation="MQNKSKSPFKIALSKFIHNKIAMLSVIFLLIITIVSIIAPLIAP
                     FPVNQQDLLNIKGEMTAQNILGTDSGGRDNFSRLLYAGRISLSIGITSTIGMLLIGIT
                     VGVISGYFGGIVDTLLMRITEFVMLFPFLIFAIVLNAALGDKIKNPYGSAIILVLVII
                     VLSWGGIARLVRGKVLQEKENEYFLAAKSIGTPTYKIILKHLLPNILSVVIVQATLLF
                     AGMIVVESGLSFLGFGISKAIPSWGNMLSDAQEGDVISGKPWIWMPPAIMITLTILSI
                     NFVGEGLKDAFNPRGRR"
     gene            complement(920..1570)
                     /locus_tag="O0V33_002395"
     CDS             complement(920..1570)
                     /locus_tag="O0V33_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002486553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8976993.1"
                     /translation="MIKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIK
                     QEAINAQREKLGLNDSIPHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLG
                     AMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQ
                     WFPFQGSVDINLKEGTFEYYMSKIYHTFLPAFTLGLLGNLISDITYGFIDPRIRSN"
     gene            complement(1563..2543)
                     /locus_tag="O0V33_002396"
     CDS             complement(1563..2543)
                     /locus_tag="O0V33_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCX8976994.1"
                     /translation="MENILEVNQVKKYYKIKTGLLQKTQYVKAVDDVSFSIKKGQTFG
                     LVGESGCGKSTLGKVIIRLEDATSGSIIVNGEDITRLQGKKLRKSRQQYQMIFQDPYA
                     SLNPMQMVGDIISEPILNYKKLPKEEIKKEVLYLLKCVGLSEDAYYKYAHEFSGGQRQ
                     RVGIARALALRPSLIVADEPVSALDVSVQSQVLNLLKDLQEQFNLSYLFIAHDLSVVK
                     HISDVIGVMYLGHIVEIASDKEIYENPKHPYTKALISSIPQIDKLNKNRIILKGELPS
                     PSNPPQGCPFHTRCPIAQDMCKKSMPELKDIGNEHQVACFYVDKVGDLND"
     gene            complement(2546..3532)
                     /locus_tag="O0V33_002397"
     CDS             complement(2546..3532)
                     /locus_tag="O0V33_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8976995.1"
                     /translation="MNNVLLEVKDLETSLKINNEWLATVENISFELSKGEVLGIVGES
                     GCGKSILSKSIIKLLPEKISKLSNGEVIFDGKRIDTLNEKQLLDIRGNDIAMIFQEPM
                     TALNPVFTIKNQLVESIKSHKKISKKEANNLAKDLLKKVGIARQDEILNSYPHQLSGG
                     MRQRVMIAMAISCSPKLLIADEPTTALDVTIQAQILDLLKELQKETQMAIMMITHDLS
                     VVAEFCDKVLVMYAGQIVEFGGIKEILHNPKHPYTQKLLSTIPKLKEEQKRLETIEGI
                     VPSIQAFHVNKCRFANRCNKKLDICNNQSPKMHVCEDVIVRCHLYQNEYKEI"
     gene            complement(3583..>5051)
                     /locus_tag="O0V33_002398"
     CDS             complement(3583..>5051)
                     /locus_tag="O0V33_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCX8976996.1"
                     /translation="DKKIHPKAYIASWKDIEPAKKIEFKIKKGIKWHDGNELKIDDWI
                     YSIEVLANKDYEGAYYPSVENIQGAKDYHEGKADHINGLKKIDDYTMQVTFDKKQENY
                     LTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYPIGIGPYKVKKIVPGEAVQLVKFDDY
                     WQGKPALDKINLKVIDQAQIIKAMEKGDIDVTNDATGAMAKDAKSSNAGLKVLSAPSL
                     DYGLIGFVSHDYDKKANKTGKVRPKYEDKELRKAMLYAIDREKWIKAFFNGYASEINS
                     FVPSMHWIAADPKELNDYKYDPEKAKKILDKLGYKDRDGDGFREDPKGNKFEINFKHY
                     SGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFGKYNEDLANASKDMEVYFRSWAGGTD
                     PDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDFDKVGIDEKKRKDIYVKWQKYMNDEL
                     PGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYNLTKEA"
BASE COUNT         1583 a          925 c          644 g         1899 t
ORIGIN      
        1 atttatgtcc cagactcttt tattatttaa cgtctacctc taggattaaa agcatcttta
       61 agcccttccc ctacaaagtt tatacttaat atagttaatg taatcattat agcaggtggc
      121 atccatatcc acggtttacc acttataaca tccccttctt gagcatcact caacatatta
      181 ccccaagatg gaattgcttt actaattccg aatcctaaaa agctcaatcc tgattccact
      241 acaatcatac cggcaaataa caatgttgct tgtacgataa ctacacttaa tatattcggc
      301 aaaagatgtt tcaaaataat tttatatgtg ggtgtaccaa ttgattttgc tgccaaaaag
      361 tattcatttt ctttttcttg aagtacttta ccacgaacaa gtcttgcaat acctccccaa
      421 cttaatacga taataactag aacaagaatt atggcagatc cataaggatt tttaatttta
      481 tctccaagtg cagcatttaa tacaattgca aatattaaaa atggaaataa cataacaaat
      541 tcggttattc tcattaataa tgtatcaaca atacctccaa aataaccaga aatcactcca
      601 actgtaattc caatcaaaag cattcctatt gtagatgtaa ttccaatgga taatgaaata
      661 cgacctgcat ataacaaacg actaaagtta tccctaccac cagagtctgt accaagaatg
      721 ttttgtgctg tcatttcacc ttttatattt aataaatctt gttggttcac tggaaaagga
      781 gctattaatg gcgctataat tgatacaata gttatgatta ataaaaaaat aaccgataac
      841 attgcaattt tattatgaat aaatttagac aatgcaattt taaaaggcga ttttgactta
      901 ttttgcattc taatcccccc tagttacttc taattcttgg atctataaat ccataagtaa
      961 tatcagaaat caaattaccc agtaaaccta aagtgaatgc aggtaaaaat gtgtgatata
     1021 ttttactcat ataatattca aaagtacctt ctttaaggtt aatatcaaca gaaccttgaa
     1081 acgggaacca ttgtaattca aaagaaaaaa taaaaattgc aaatactccc gcaataaaag
     1141 atggaatggc taacatcaaa taattaaata tttgcacagt ataatccgtc aaactgtaag
     1201 aatatctacc tgacgttatt cctaaagcaa atgagataat ataagtaata attaatgaca
     1261 tagcaccgag taatattgta tttggaattc tttcctcaat aacatcaatt accggccttt
     1321 tatatttgat tgactcccct aaatcaccgt gtataacatg attgacccat ctaatgtatt
     1381 gatgtggtat tgagtcgttg agacctaatt tttccctttg tgcatttatt gcttcttgtt
     1441 ttattttacc gttttgtaga tctgaaaatg gatctcctgg ttgtatgatt gctaatgaaa
     1501 atacaactat tgaaattaaa attagtaacg ggatcattaa ccctaatctt ttaagtatta
     1561 atttaatcat ttaaatcacc tactttatct acgtagaaac aagcgacttg atgttcgtta
     1621 ccaatgtcct ttaattctgg catacttttt ttgcacatat cttgagcaat tggacatcta
     1681 gtatggaaag gacaaccttg tggcggatta cttggcgaag gtaattctcc ctttaatata
     1741 attctatttt tatttagctt atcaatttgt ggtattgatg aaatcaacgc ttttgtataa
     1801 ggatgtttgg gattttcata aatttcttta tcagatgcga tttcaactat atgacctaaa
     1861 tacataactc caatgacatc acttatatgt tttactacac ttaaatcatg tgcgataaat
     1921 aaatagctta agttaaattg ttcttgtaaa tcttttaata aattcagtac ttgagattga
     1981 acagatacat ctaatgcact tacaggctca tcagcaacaa ttaaactcgg acgcaaagcc
     2041 aatgctcttg caattcccac tctttgtctc tgtccacctg aaaattcatg tgcatattta
     2101 taatatgcat cttcacttag accaacacat tttaataaat atagaacttc tttttttatt
     2161 tcttcttttg gcaatttttt ataatttaaa ataggttctg aaatgatatc tccaaccatt
     2221 tgcatcggat tcaatgatgc atacggatct tgaaatatca tctgatattg ttgtcgtgat
     2281 tttctcagtt ttttaccttg taatcttgtt atatcttcac cattaacaat tattgagcct
     2341 gaagttgcat cttcaagtct gataatcact ttacctaacg ttgacttacc acaacctgat
     2401 tcgcctacca atccaaaagt ttgtcctttt tttattgaaa acgatacgtc atcaacagct
     2461 ttaacgtact gagttttttg taataatcca gttttaattt tgtagtattt ttttacttga
     2521 tttacttcta aaatattttc cattcttata tctccttata ttcattttgg tataaatgac
     2581 accgtacaat gacgtcttca caaacatgca ttttaggaga ttgattatta caaatatcca
     2641 gttttttgtt acatctattt gcaaatctgc acttattaac gtgaaatgct tggattgatg
     2701 gcacaattcc ttctattgtt tcaagtcgtt tctgctcttc tttaagtttt ggaattgtag
     2761 ataataattt ttgggtataa ggatgtttcg gattgtgtag tatttctttt atgcctccaa
     2821 attctacaat ttgacctgca tacataacca aaactttatc gcaaaattca gcaaccacac
     2881 tcaaatcatg tgtaatcatc ataattgcca tttgcgtttc cttttgcaat tcttttaata
     2941 agtctaatat ttgcgcttga atcgtgacat ccaaagcagt tgtaggttca tcagcaatta
     3001 ataatttggg agaacatgaa atggccattg caatcattac tctttgcctc ataccaccag
     3061 ataattgatg gggatagcta tttaatattt catcttgtct tgcaattcca acttttttta
     3121 gtaaatcttt tgctaagtta tttgcttctt ttttagaaat ttttttatgt gattttatag
     3181 attccacaag ttgatttttt atagtaaata ccggatttaa agcagtcata ggttcttgaa
     3241 aaatcatagc aatatcattt cctcgaatat ctagcaattg cttctcattg agcgtatcga
     3301 ttcgtttacc atcaaatata acttctccat tacttagttt agatatcttt tctggtaata
     3361 atttaataat tgacttactt aatatggact taccgcaacc agattcccct actataccca
     3421 aaacttctcc tttagataat tcaaaagaaa tattttcaac agttgctaac cattcattat
     3481 ttatttttaa tgatgtttct aaatctttaa cctctaacaa tacattattc ataacatccc
     3541 cccatataag taaacctcga caaaatcgtc gaggttacat tactaagctt ctttagttaa
     3601 attatataaa ctttgatcag ttccaatttc aatgtctaag tttcgtactt tatcattaac
     3661 tatagttatc gatttacctt ggaacattgg taatccaggt aactcatcat tcatatattt
     3721 ttgccattta acataaatat ctttacgttt cttttcatca atgcctactt tatcgaagtc
     3781 taatgcatca tctaaatatt gatctgattt tggtaaaact gttctcattt cattttgagg
     3841 tctatcagtg tggtataaat ctgatgggtc tggatctgta ccacctgccc atgatctgaa
     3901 gtacacttcc atatcttttg atgcatttgc taagtcttca ttatatttac cgaattctac
     3961 taacttcaca tttgttttca agccaacttt ttcccagaaa tcttttatcg cagcagttct
     4021 tggttcaaaa gtaggatttg aacctgaata atgtttaaag ttaatctcaa atttattacc
     4081 tttaggatct tctctaaatc cgtcaccatc tcgatcttta taacctaact tatctaagat
     4141 ttttttagct ttttcaggat catatttata atcgtttagt tccttaggat cagctgctat
     4201 ccaatgcatt gatggtacaa aactatttat ttcactagcg taaccattga aaaatgcttt
     4261 gatccatttt tctctatcaa ttgcataaag cattgcttta cgtaattctt tgtcttcata
     4321 ttttggtctc actttaccag ttttattagc ttttttatcg taatcatgag atacgaatcc
     4381 tattaaaccg tagtctaagc ttggcgcaga taataccttg agaccagcat tagatgactt
     4441 agcatctttt gccattgcac cggtagcatc attcgtaaca tcaatatcgc ctttttccat
     4501 tgccttaata atttgagctt gatcaataac ttttaaattg attttatcta atgcaggctt
     4561 accttgccaa taatcatcaa atttaacaag ttgaacagcc tcacctggga cgattttctt
     4621 aactttatat ggtccaatac caataggata ttttcggatt ttatctgatt tcgctaaatc
     4681 tttaattggt acatctgata aatatttttt acttaataaa ggtccagtaa taaatcctgt
     4741 taagtaattt tcttgctttt tatcaaatgt aacctgcatt gtgtagtcat ctattttctt
     4801 caatccgtta atatgatcag cttttccttc atgataatct tttgcacctt ggatattttc
     4861 tacacttgga taataagcac cttcgtaatc cttgttagct aagacttcaa ttgaataaat
     4921 ccaatcatca attttcaatt cattaccatc atgccattta ataccttttt taattttaaa
     4981 ttctattttc ttagccggct cgatatcctt ccatgaagca atatatgctt taggatgtat
     5041 ttttttatca a
//
LOCUS       DAKSQE010000140         5047 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.140, whole genome shotgun sequence.
ACCESSION   DAKSQE010000140 DAKSQE010000000
VERSION     DAKSQE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.140"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            99..815
                     /locus_tag="O0V33_002399"
     CDS             99..815
                     /locus_tag="O0V33_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCX8976997.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIGKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            815..1288
                     /locus_tag="O0V33_002400"
     CDS             815..1288
                     /locus_tag="O0V33_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCX8976998.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYAVPK
                     GTIFASSIEISNTYSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            1380..1496
                     /locus_tag="O0V33_002401"
     CDS             1380..1496
                     /locus_tag="O0V33_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8976999.1"
                     /translation="MLQLILMKIALNDFEKDKSVLFIFLKNHFYEEMCVLSN"
     gene            1641..2285
                     /locus_tag="O0V33_002402"
     CDS             1641..2285
                     /locus_tag="O0V33_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCX8977000.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKIHQKPQNKTNFEKSKQIKPKQYNQTKNHQQQKKN"
     gene            2403..3269
                     /locus_tag="O0V33_002403"
     CDS             2403..3269
                     /locus_tag="O0V33_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCX8977001.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKQWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMTSFVEEPKA"
     gene            3401..4621
                     /locus_tag="O0V33_002404"
     CDS             3401..4621
                     /locus_tag="O0V33_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HCX8977002.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFF
                     TKNQKITKEDVIST"
     gene            4618..>5047
                     /locus_tag="O0V33_002405"
     CDS             4618..>5047
                     /locus_tag="O0V33_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HCX8977003.1"
                     /translation="MTIVLWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLSPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQ"
BASE COUNT         1749 a          662 c          948 g         1688 t
ORIGIN      
        1 gtagttcaat aatgttaatt gtgaaaatgt atataatcat aaaaaaatca tgtataatat
       61 atgttgttaa ttaaacagtt cgaaagcgag atgacattat gggacgtaaa tggaataaca
      121 ttaaagaaaa aaaggcccaa aaagataaaa atacaagtag aatatatgcg aaatttggta
      181 aggagattta tgttgcagca aaatctggtg aaccaaatcc agaatctaac caagctttaa
      241 gattggtact tgaacgcgct aagacatatt cagtaccgaa tcatattatt ggaaaagcaa
      301 tagataaagc taagggtgct ggagacgaaa actttgatca cctaagatat gaaggatttg
      361 gcccaagcgg atcaatgctt attgttgatg cgttaacaaa caatgtaaat cgtactgcat
      421 ctgatgtgcg tgctgctttt ggtaaaaacg gcggtaatat gggtgtatct gggtcagttg
      481 cttatatgtt tgaccatgtg gcaacatttg gtattgaagg taaatctgtt gacgaaatac
      541 ttgaaacatt aatggaacaa gatgtagatg taaatgatgt gattgacgat aatggattga
      601 caatagtcta tgctgaacca gatcaatttg cagtcgttca agatgcgctt cgtgcagcag
      661 gtgttgaaga atttaaagtt gctgaatttg aaatgttacc tcaaacagat attgagcttt
      721 ctgaagcgga ccaagtaaca tttgaaaaat taatcgatgc attagaagat ttagaagatg
      781 tacaaaacgt attccataat gtggatttga aataatgaaa tcagcagaac aatggattga
      841 tgaattgcaa cttgaatcgc atcctgaagg tggtttctat agagagacaa tccgagaagt
      901 attgaaagat ggacgaagag cgccgtttag tagtatttat tttttgctta cagatgacaa
      961 tatttcgcat tttcatcgaa ttgatgctga tgaagtatgg tactatcatg ctggtgattc
     1021 tctaacaatt catatgataa atccggatgg ggaatatacg actgcaacat tgggtactga
     1081 tatccaaaat ggagatgtat tgcaatatgc agtgcctaaa ggaacaattt ttgcttcttc
     1141 aatcgaaatt tcaaatactt atagtttagt aggttgtatg tgtcaaccag catttgagtt
     1201 taagcagttt gaattgttta agcaatctga attaattaca caatatccgc atcttaaatc
     1261 agtaatcgaa aaatatgctt taaaataaaa agaatcaatg aagtgatttg aaggttgtta
     1321 gtaaaccttt gagtcacttc atttttgtat gtattcttga ttgaatcaga atagatttgg
     1381 tgcttcagct gattttaatg aaaatagcat taaatgattt tgaaaaagat aagagtgtgt
     1441 tatttatatt tttgaaaaat cacttttatg aagaaatgtg tgtactatct aattaatatt
     1501 gtctatttta agtaaattga agcgagttgt agtaaaataa taataaatat tttcatatga
     1561 ttaacaaaaa ctataaaact gtatcatgac atgacatcat ccatgtagtt ttatataata
     1621 atgacaagga gttggtgact gtgaaagtaa agtatataga taaacgtcac tggcgtcgcc
     1681 taattgatag ggaatacaca gaggtaaaag ttaataataa taggttcaag ggtattatag
     1741 gcttagtcac gatgaaaaag gttcgtgatc ctttagaggt gacggtagtt ggacaaaata
     1801 ttattgtcgc agatgacaat tataaatggt tgcaaatact acctgaaaag aaacgttata
     1861 gtattactgt aatgtttgat aataaaggca atccattaga atattacttt gatataaata
     1921 ttaaaaatat aacgcaaaaa ggtaatgcgc gtacagtaga tttatgttta gatgttttag
     1981 cattacctag tggtgaatat gagttggtag atgaagatga tttaatgttt gcattagaaa
     2041 gtgagcaaat tacaaaaaag caatttcatg aagcatatat gattgcgcat caaattatgg
     2101 cagagttaga aaatgatttt aaaggattcc aaaagaaaat catgtactgc tttaataaaa
     2161 ttaatgcaaa ggctcaaaaa attcatcaaa agccacaaaa taaaactaat tttgaaaaaa
     2221 gcaaacaaat aaaacctaag caatataatc aaactaaaaa tcaccaacaa caaaagaaaa
     2281 actaagtaat tcaagctgca gccataccaa taaaattggt gaaaattatt cgttagtaat
     2341 gaactcttga atgctcgtag tcatgaaagt tcaataattg aataatttat gggggggaaa
     2401 ttatgaagat tgaagactat cgtttactaa taacattaga cgaaacgaaa acgttacgta
     2461 aagcggctga aattttatat atatctcaac ctgctgttac acaaagacta aaagctattg
     2521 aaaatgcttt tggagtagat atttttatca gaacaaaaaa acaattgatt acaacaactg
     2581 aaggaacaat gattattgag catgcccgtg acatgttgaa aagagagcga ttattttttg
     2641 acaaaatgca ggcacatatt ggtgaagtga atggaacgat atcaatcggg tgttcttcat
     2701 tgattggaca aaccttactt cctgaagttt tgagcctata taatgcccaa tttcctaatg
     2761 ttgaaattca agtacaagtt ggttcaactg aacaaattaa agcaaatcat agagattatc
     2821 atgttatgat aactcgtgga aataaggtaa tgaatttagc taatacacat ttatttaatg
     2881 atgatcatta ttttattttt ccgaaaaata gacgagatga tgttacaaag ttaccattta
     2941 tagagtttca agctgatccg atttatataa atcaaataaa acaatggtat aacgataatt
     3001 tagaacaaga ttaccatgca actattacag tggatcaagt agcaacttgc aaagaaatgt
     3061 tgattagtgg tgtaggtgtt acaatcttgc cagaaatcat gatgaaaaat atcagcaaag
     3121 aacaatttga gtttgaaaaa gtagaaattg ataatgaacc gctgattcgt tcgacattta
     3181 tgagttatga tccgagtatg ttgcaattac cacaagttga ttcatttgta aatcttatga
     3241 cgagctttgt tgaagaacca aaggcgtagt cttagactaa tttaaggtta gtatttaatt
     3301 ttaagatata cggttaaatt gaacacagtt ggatgctaat gcaccaacag caaaagagcc
     3361 cctaattaat aaattaaaag gggctctaag aagcggggtt atggatatgt ttgcagcgtt
     3421 attacaaata aagaattata aactctttgt tgctaatatg tttctattag gtatgggtat
     3481 tgcggttacg gtcccatatc ttgttctttt tgcaactaaa gatttgggta tgacaacaaa
     3541 tcagtatgga ttacttctag catctgcagc gattagtcag tttacagtaa attcaattat
     3601 tgctagattt tcggatacgc atcactttaa tagaaaaatt attattattc tcgcattatt
     3661 aatgggtgcg cttggttttt caatatactt ttttgtagat acaatctggt tattcatatt
     3721 actatatgcg attttccaag gattatttgc accagcaatg ccccaacttt acgcatctgc
     3781 tagagaatct atcaatgttt caagctctaa agatagagcc caatttgcca atacagtatt
     3841 acgttcaatg ttctcattgg gctttttatt tggtccattt attggtgcgc aattaatcgg
     3901 attaaaaggc tatgctggat tgtttggtgg aacaataagt atcattttat ttactttagt
     3961 acttcaagtg tttttctata aggatttaaa cattaaacac cctattagta cgcaacaaca
     4021 tgttgaaaag attgctccta atatgtttaa agacaaaacg cttttattac catttattgc
     4081 atttatttta ttacacattg gacaatggat gtatacgatg aatatgcctt tatttgttac
     4141 tgattattta aaagaaaatg agcaacatgt cggttattta gctagtttat gtgctggttt
     4201 agaagtgcca tttatgatca ttcttggcgt tttatcatct agattacaga ctcgaacatt
     4261 gttgatttat ggagcgattt ttggaggttt attctacttc agcattgggg tatttaaaaa
     4321 cttctatatg atgttagcag gacaggtgtt tttagctatt ttcttagcgg ttcttttagg
     4381 aattggtatt agttatttcc aagatatctt accagatttc ccaggatacg cctcaacact
     4441 attttctaat gcaatggtta ttggacagtt aggcggtaac ttattaggtg gtgctatgag
     4501 tcactgggta ggtttggaaa atgtattttt tgtatcagca gcatcaatca tgttaggtat
     4561 gatacttata ttctttacta aaaatcaaaa aattacaaaa gaggatgtga tatcaacatg
     4621 acaattgttt tatggctact tatcatcgct gccttcatgt tagcatttgt tgggttgatt
     4681 aagccgatta ttccttctgt tttagtatta tgggttggct ttctaatcta tcaatttggc
     4741 tttcataatc agcatttatc atgggtgttt tatgtatcta tggcattgct aacaatatta
     4801 attttatgtg cggacttttt agctaataaa tattttgtga atcgcttcgg tggttctaag
     4861 tttggagagt atgcagcttt aattggtgtc gttattggat gttttgtttt atcgccattt
     4921 ggaattatta tcattccatt tattttggta ttcatagttg aattaataca aggctattca
     4981 tttgaaagag cagttaaagt aagtataggt tcaatcgtag catttttaac aagtagtata
     5041 gctcaag
//
LOCUS       DAKSQE010000141         4974 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.141, whole genome shotgun sequence.
ACCESSION   DAKSQE010000141 DAKSQE010000000
VERSION     DAKSQE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.141"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(114..2723)
                     /gene="clpB"
                     /locus_tag="O0V33_002406"
     CDS             complement(114..2723)
                     /gene="clpB"
                     /locus_tag="O0V33_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCX8977004.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRSLVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDSDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
     gene            complement(2926..4740)
                     /locus_tag="O0V33_002407"
     CDS             complement(2926..4740)
                     /locus_tag="O0V33_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCX8977005.1"
                     /translation="MNKTKGFTKYKKMRYMPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLIS
                     ILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     YVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRSSYIPQFIRMVIVVTLLIPFML
                     ILVGAFNKYGKDIIGEKANSFDTTIEDNYSMRIAPIDNIHIDGLVSEKKKESSDVYNN
                     IKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVL
                     ELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLID
                     WYKRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK"
BASE COUNT         1562 a          931 c          638 g         1843 t
ORIGIN      
        1 aatattggcg tttattaaat catttaaaaa gtatattgcc aatgaacaat tataataaat
       61 ttcaaatcta aaaaaccaag aatgcgatta atcatcacat tcttggttca attttattca
      121 tgaatttttt caacattaaa cgttaagtta ttgtctgaat ttaaattaac tttaatagtt
      181 gtaccttctg ggaatccctc tttaatcatc atacgtgcta atggtgtttc aatttggcgt
      241 tgtacaaaac gttttaatgg tcttgcaccg tattgaggtt cataagcctc ttgacctagc
      301 caagctttag catcatcaga aacttcaatt gagattcgtt gttctagtaa tcttatattt
      361 aattgcgtta atattttgtc tacaatcata ctcatgtcat caatagataa aggtttaaat
      421 aatacgatgt catccatacg attcaaaatt tctggtttga aatatgcatt taaacttgtc
      481 ataacagctt tttctgttga ttctgtaatt tcaccagtct cttttacgtt ttctagtaaa
      541 acttgagatc caatattact tgtcataata ataatagtat ttttaaaatc aacgctacgt
      601 cctttagaat cagttaaacg gccttcatct aaaatttgca ataatacatt aaagacgtca
      661 gtatgcgctt tttcaacctc atctaataaa ataactgagt atggattacg acgaaccgct
      721 tcagttaatt gacccccttc atcatgtcca atatatcctg gaggtgcccc tatcaatctt
      781 gatactgcat gtttttccat atattcactc atatcaatac gaatcatatg tttttcagaa
      841 tcaaataatg atgcagctaa tgatttagct aattcagttt tacctacccc agttggacct
      901 aggaataaga aactaccaat aggtctgttt ggatctttaa tacctgctct agctctaact
      961 actgcatctg aaaccaggtc aaccgcttta tcttgaccta caacacgttt atgcaagata
     1021 tcacttaagt gaagtaattt ttcacgttct gtttcaacta attttgaaac tggtatgcct
     1081 gtccattggc tgacaatatc accaatttct tcgtctgtta caacttcacg aatcattcga
     1141 tctgaatctt caccttgctc atcttggaaa ttatcctcta attctctaag ctctttttcc
     1201 aattgaggaa ttgttccata ttgtagttca gctgcttttt ctaaattgtt atttgtttgt
     1261 gcatcttcca acgcttgtct actttcatct agttgcgcac gtttttcttg taaatttgct
     1321 attttttctt tttctgattc tacacgagat tgaagtgctg cttgtttctc tttttcattg
     1381 gcaagctctt cttgtagttc ttgtaatctc tgtttgctcg cattgtcaga ttcattttta
     1441 agtgcgcttt cttcaatttc taattgcatg acacgtctat taacttgatc caattcagtt
     1501 ggatttgatc ccatttccgt acgaattgtt gcacatgctt ggtcaactaa atcaatcgct
     1561 ttatctggta aaaaacgatc agtgatgtaa cgatcagaca attcagcggc agcaactaag
     1621 gatctatctt gaatacgcac accatgatat acttcatatc gttcttttaa accacgtaaa
     1681 attgaaattg tatcttcaac atcaggctca ctaactgcta ctttttggaa acgacgctct
     1741 aatgccgaat ctttttcaat atattctcga tattcattta aagttgttgc accaatacaa
     1801 tgtaactctc ctcgtgctaa cattggtttt aacatgttgc ctgcatccat ggcaccatct
     1861 gttttaccag cacctacaag catatggatt tcatcaataa ataatataat tctaccatca
     1921 gactctttaa cttcttttag gactgctttt aatctctctt caaattcacc acgatattta
     1981 gcgcccgcta ctaatgcgct taaatctaac tcaaaaacag ttttatctaa taatgattct
     2041 ggcacatctt tcttaactat acgctgcgct aatccttcaa caattgcagt tttaccaaca
     2101 cctggttcac caatgagcac agggttgttt ttagttttac gacttaaaat acgaatcgta
     2161 tttcgaattt cttcatctct tcctataaca ggatccattt taccttgtct aacttcttct
     2221 actaagtcgc ggccatattt agctaatgct tcgtaattaa cttctggatt ttgtgatgtc
     2281 acgtgatttc cccctcttac ttttttaata atttctttga taacttctac tttattattt
     2341 atataatgtt ttgttgtttg atcaatgtcc attgccgaac gtaaaatatg ctccattgaa
     2401 atatattcat cttcatattc tttcatgtat gattcagcct tagttatcaa ttggtttgct
     2461 tgttggctaa tatattgacc atattgtata ttgtcacctt ctacagatgc atacgtgttt
     2521 agtttgtctt cataagcttt gtttaattga tctacctcaa tatttgctcg ttctaaaata
     2581 cttttatata agctttcact ttcatttaag gcagcgctta aaattgcctc aatttctata
     2641 ttttgtaatt tatgttgctg actcagttca actgcttgtt gtaaagcact ttgaacagca
     2701 tatgtcattt tatttatatc cattaatttc acctctaatt tgtagagttg gtcaagttgc
     2761 acttttgact ttgactttct ttgaccttaa ttatattata gtcccaatat aatcatttat
     2821 caactctttt acacttaaat ttcctaatat ttgtaaataa ttttttgaat taaaaaaatc
     2881 tagctaatgt tacgttacac attaactaga cttattaaac atatgttatt tctttttaag
     2941 tgcttttaat atttcatctt ttaaagctag gactcctttg tactctaaat gtacaccgtc
     3001 tggtgcaaaa tattcactat gtccttgtga tcgcttatac caatcaatta atgtgacatt
     3061 ggactttcgt ttcgccgcgt cagctaataa tcgatttaca tttgcctcat aaattcttgg
     3121 aacacgtgta ttaactaaat aaatcttggc ttttccaaat tgattaagta aatcgtcgag
     3181 ctgtttgaca gtaaagtcgc catttgtacc taattctaat acgacttgat cagatgattt
     3241 tttatattgt gaataattcg ctttaactaa aggtaaggtt tgatacaatt gacgccctac
     3301 ttttccatca attctagact taggaactga tgacttaaat gactcaccga tatcaaccat
     3361 tactgaatca ccgattaaaa gaggtttaat attattatat acatcggaag attccttttt
     3421 cttctcactt actaagccat caatgtgaat gttatcaatt ggtgctatcc gcattgaata
     3481 attatcttca atagtggtat caaagctatt cgctttttct cctataatgt ctttaccata
     3541 tttattgaat gcacctacta aaatcaacat aaatggaatt aacaaggtta ctacaattac
     3601 cattcttata aattgtggta tataagaaga tcgccaattt aaagctttaa taccttcttt
     3661 tctaaatgga gtttctataa agcgatatga tagctctgca aatataattg ttaaacttat
     3721 atctataaag tacacatata caggtatctg tccgtctaca tagtaactat gtacgaaact
     3781 aattactgca aaatgccata aatatagact ataagacctt ttcccgataa atactaacac
     3841 tggatttgaa aatatcttcg ctatccatgt agatggatga acgacactgg caataataaa
     3901 taacgttaat atggatatta aatagaaacc accatcatat atccaattcg tctcatcatt
     3961 aataatgaaa aataataata taagtactat aaatgataaa ctacctatgc tatcaataac
     4021 atattttaca actttaggtg gatcattttt caatttaaac ggcggccata aaaaagctaa
     4081 aataacaccc agtaacaatg tctgtaatct agtatctgta ccaaaataca ctcgtgaatg
     4141 atccccatta atactgtaga taaacatcat taaccctaaa gaaattatag atactcccca
     4201 aaaaataaat cctattttgt atcgcttttt aattgttaac aataatgtaa ccaaaataac
     4261 tgggaaaaat atgtaaaact gttcttcaat tgctaaagac cataaatgct ttaatggcat
     4321 aaatgaaaat tgctcaaaat aattaacatc ttttgctata taccaccagt ttgatacata
     4381 aaatatcgca gcaataatat catgtttaac cctaatgata ttatctgatt ttaataataa
     4441 ggttgctgtc cctacaacca ttaataaaac tatgactgct ggtaataaac gttttaaacg
     4501 acgtatccaa aagcttttca atttaatgat acctgtgtca tcatactctt tgagtaataa
     4561 gcttgtaatt aaataaccag agatcacaaa aaatgtatcc acacctaaaa agccacctgt
     4621 caaccattgc ttatttaagt ggtaaataat aattcctaga acagcgattg ccctcaaacc
     4681 atcgagccct ggcatatatc tcattttctt atattttgta aaaccctttg ttttgttcat
     4741 ttttgcattc ttccctttta aaactgttct tcttagatgc ttaattaaat ttagttatgc
     4801 tgtttaaaag aatattgaaa tgcatatgta tattattgaa ttacgacatc atcaaaatca
     4861 tattgactaa aatactgtta aattaaaaaa ttaccaatga tgattcttat ttcgaaatcc
     4921 aatttgtaat gcaactcggc aacttaaaaa ctatgaagta ttatgtattg taat
//
LOCUS       DAKSQE010000142         4964 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.142, whole genome shotgun sequence.
ACCESSION   DAKSQE010000142 DAKSQE010000000
VERSION     DAKSQE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.142"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            55..249
                     /locus_tag="O0V33_002408"
     CDS             55..249
                     /locus_tag="O0V33_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977006.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDAPKFGCVNGCMLFILY
                     YRVNRIGGNRSFEIFTNPIF"
     gene            complement(318..1127)
                     /locus_tag="O0V33_002409"
     CDS             complement(318..1127)
                     /locus_tag="O0V33_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCX8977007.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYSYNNYNNGYSYNNYSR
                     YNNYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSG
                     YTSGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQT
                     TQGAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
     gene            1737..3692
                     /locus_tag="O0V33_002410"
     CDS             1737..3692
                     /locus_tag="O0V33_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCX8977008.1"
                     /translation="MTDNYSLHIFKSMKMSSVKEANLKIIYWFQGTGVVSINLQQYDV
                     KRNDVTVIMLNDLYQIDGHDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSKIKQQPYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMFKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGDTPKLIRKRNSPTSHSINHSQLVEIDKNIIELFDDTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNPENLHIIVT
                     IDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSTERGKIEKLVATIFNMTF
                     ESNRCKIAFLINDLNTTAIQKLKRLIDNYIGVFELIYHLEPNELNTRYDKEIDHLIDY
                     FILPLRQIEQVSIARSKIICDVDLINENNDLKEKITMSDINKVTELLTNYTKFRGLIM
                     HMSIISEASKEINLTRLSIFIFIINILNQLRGIVIYQNDTMIVTKFKHEVQCVICLPA
                     ALLNENSDAIRLSCKGLQQFSHAKTKQIVLSIDKDNHTSENGVLVNDIMQQHSYWKAN
                     FIERNIYDHSLMVSSNSIVHLKYHLEI"
     gene            3718..4461
                     /locus_tag="O0V33_002411"
     CDS             3718..4461
                     /locus_tag="O0V33_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCX8977009.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDRLLKMKEIQSKMALLQMLSYLNNHQWLLKARDQRDQRKLTISINKMHI
                     DKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELLLNLKCYLNMCQLSLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVKLNNK"
     gene            4525..4851
                     /locus_tag="O0V33_002412"
     CDS             4525..4851
                     /locus_tag="O0V33_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCX8977010.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYQVQGANEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSNNVEMFVEYV
                     KQHMIV"
BASE COUNT         1772 a          607 c          867 g         1718 t
ORIGIN      
        1 tttgtagcgg aacatgttaa tatatgtaat gtaagcttta tgtcatggca tgtaatgact
       61 aaattaattg agaatttgaa ggcaagtata tttgtaagta ctttaactaa aaatttatca
      121 atgtatagcc gatttgacgc gcctaaattt gggtgtgtca atggctgtat gttgtttatt
      181 ctttattaca gagtgaatcg gattggtgga aatcgaagtt ttgagatttt taccaatccg
      241 attttttgat gtctagaaat taaaaaagcc aacaaggctc ttgaaacctt gttggcttaa
      301 ttcactttca tcacaaatta gtgaatgaag ttataaccag cagcttggct agctgagatt
      361 gtacgtgaag ttacaacacc tgggccataa ccatagttca tttctgaaac tcttactgaa
      421 ccattgctgt taacactttc aacgtatgca acgtgaccgt atgcaccttg agttgtttgc
      481 ataattgcac cagcttttgg tgtattgttc actgtgtaac cagctcttgc agctgcgtta
      541 gcccagttac ttgcattgcc ccaagttgaa ccgattttac cacctacacg atcaaatacg
      601 tagtatgtac attgaccaga agtgtataag ttacgtcctg aagtataacc acttgagatt
      661 gaacggccat ttgatgatgg agccatagtt gtagttactt gaacattgtt gcttgaagtg
      721 ctgtagcttg cacctaaacc accagtacgg tagctgtttg tgttgtaact attatagttg
      781 ttgtagttat atgattgatt attatttgag tagttgttgt aacggctgta gttattgtag
      841 ctataaccgt tgttgtaatt gttgtagcta tagttattgt agccattgta gtagtaatag
      901 ctgtagtagc cattatcttg gtttaattga cttggatgcc agttaccttt ccatgtgtaa
      961 tggtagttac cttgtgcatc aatagtgtaa gtatagctat atgatgttgg gtcatttgga
     1021 ttataaccgt agttatcttg ctcagaagca tgagcttgat ttcctgatgc aattgcgatt
     1081 gtagcgaatc ctgcagttgc gatagtagct gtagcgattt tcttcatttt aaaaatatcc
     1141 tcctaaaaat tttaaatcta aaatattttc gtaatgtccg tgtgacaaaa ttaatgttat
     1201 aagttatctc tcgtaattaa acgacaagaa agactataac agaaattagc gtccttgtgt
     1261 gctttgttaa cgttttgtaa ttttttgcta atatcttgac acaatagaat tgtaaaagta
     1321 tagaaatttg cattttgcaa aacttataac tacggcattc tttgtaaaaa ctgaatgttt
     1381 cgaaaataag tatgttacaa atttgtaata ttactgaaaa ttctaaatgt atattttgtg
     1441 cataatatag gacttttaat cagaaaatgc ataaaaattc gacacgaacg atcaaatttc
     1501 tttggttagg aaagctttgg tatttggata aaaatgtata tttaatggat aaaatatcct
     1561 aatttagcat aaaaaaatgt tttaataaaa gtattatttg atataatcga tttatgtttt
     1621 gttactgcta aaaaacatgt ggcgatataa ttggtagtga atattagatg ttgacaattg
     1681 atttgagaaa gacaaacagg agtaaatcaa tatatttgta tatgtgagga tgtaagatga
     1741 cagataatta ttcactacat atttttaaaa gtatgaaaat gagtagtgtg aaggaagcaa
     1801 acttaaaaat tatatattgg tttcaaggta caggtgtagt ttcaatcaat ctacaacaat
     1861 atgatgtgaa aagaaatgat gtcaccgtta ttatgttgaa tgatttgtat cagattgatg
     1921 gtcatgatga tagtgtatgt tgtgttgttg aagtaagtgc aaagacattt ttgaagttta
     1981 tgaatgcaaa ctatatgatg cgtggcaaga tattaacaaa tgaggttgct agcactttta
     2041 gaatcttgat taagtattta atctacagca aaattaaaca acagccttat aatgctaatg
     2101 ataagattat taattatatg tgtgttgaat taatgtcatt aaatacatac gatggtagtc
     2161 gtcatttagt agccgaagaa gtgcacgatt atttaacaaa caaccaccat aaaaagataa
     2221 ataggaaaga tgtcattaat caagttagta tcacaaacaa agctttaagt gacatgttta
     2281 aagcgacgcc ttatagtaat tttattcaat atttaaatca tattagactg gagcattgct
     2341 taattgatat tttaacttct aagaagccca ttgaagaaat cgcaagtagt catggtttta
     2401 atcattattc tcgttttata cacctcttta aagaaacgta tggtgacact cctaaattaa
     2461 ttagaaaaag aaatagtcca acatctcatt caattaacca ttcccaatta gttgaaattg
     2521 ataaaaatat aattgaatta tttgatgata ctaatcaaag ttcttcaagt atgcgcgaga
     2581 ttgatatacc ttttgagcct acaaaaagga gtcaaatgta tcaacctaaa aacatctata
     2641 ttaaaggaag taatttttat tggatggatt actatactat taaacaaatc attcaaagat
     2701 taggtgttaa tcctgaaaac ttgcatataa ttgttacaat cgacacaagc aatgagctat
     2761 cgattgaaca aatcacttta ttacttcaaa agatagtgaa ttacaatgta aatgtagtat
     2821 ttcgtattaa acatgaatat aaggaattgt taacgagtac tgaaagaggc aagatagaaa
     2881 agctagtagc tacaattttt aatatgactt ttgagagtaa tagatgtaag attgcatttt
     2941 taattaacga cttaaatact actgcaattc aaaaactgaa acgtttaata gataattata
     3001 taggagtatt tgaattaata tatcatctgg aaccaaatga gttgaatacg cgctatgata
     3061 aagaaattga tcatctaata gactatttta tcttaccgct aagacaaata gaacaggtgt
     3121 ctattgcacg ttctaaaatt atatgtgatg tagatttgat taatgagaat aatgacttaa
     3181 aagaaaaaat cacaatgtct gatattaata aggttactga acttttgaca aattatacaa
     3241 aatttcgagg acttattatg cacatgtcaa ttataagtga agcgagcaaa gaaataaatt
     3301 tgactagatt atcaatattc atttttatca taaatatact taatcaatta cgagggattg
     3361 ttatttatca aaatgacacg atgattgtaa cgaagttcaa acatgaagta caatgtgtca
     3421 tttgtttgcc agccgcattg ttaaatgaga attcagacgc tatacgttta tcgtgtaagg
     3481 gtcttcaaca atttagccat gctaaaacta aacaaattgt tttaagtata gataaagata
     3541 atcatacttc cgaaaatggt gtacttgtga atgatattat gcaacaacac agttattgga
     3601 aagctaattt tatagaaaga aatatttatg atcattcatt aatggtctca agcaatagta
     3661 ttgttcattt gaaatatcat cttgagatat aggcatggtt taagagaggt tttaaagatg
     3721 tctaaattta aagttaagcg tttaactgca cacatactaa tgttgaaagt cattaatgat
     3781 atgttgaaat attcatttca tctcactttg actcaagtga aactattaaa tctattagtt
     3841 aaatatgata attctgattc caatagcgct tcaatagata ggttattaaa aatgaaagaa
     3901 atccaatcta agatggcact tcttcaaatg ttaagttatt taaacaatca tcaatggcta
     3961 ttgaaagcaa gagatcaaag ggatcaacgt aaattgacta tttcgattaa taaaatgcat
     4021 atagataaaa tagaatatat gaataatgag cttagtgact atattgaacg ttattttggt
     4081 caatatacat ttgattttag ttgtgactat gtatcgtatt tattgtcgtc acaagaatta
     4141 ttgctaaatc taaagtgtta tttaaatatg tgtcaattat cattagaaga gttatacgtt
     4201 ttaggaatat taaatctgca taaaggtcaa ttgacggtta aagaattaca gggtgaattc
     4261 catcacccga tatttgcggt tagtccgatt ttaaagtgtt taattttgaa aggattagtt
     4321 aaaaaagaga gatgtgaatt agatgaaagg cgtgttattg taacaatcaa gcgggaaaag
     4381 ttttctaaag tcactatgct tattcaagca tgttataatt atcttgaaaa gggaatacaa
     4441 gttaagctta ataataaata gttaatcact tgtatttcat atacctatga agaatagttt
     4501 atagttttaa gtgggaggga tattatgatt ttagataatg tgaatccaaa tgatttattt
     4561 ccaactgaaa aaaagggtcc gtcagtgtta ggaataattg agtatcaagt tcaaggtgcg
     4621 aatgaatttg aaggggcatt tatagcaaca aatgaaagat taattatgaa tgtagatatg
     4681 aatggccaat tttattatag aagtattagt tataatgaaa tagaaaagat agattatgat
     4741 ggtcaaacta taatgttcaa atttaatatt ggtaatgtcc caatgcatga tattaaaagt
     4801 aataacgttg aaatgtttgt tgaatatgta aaacaacata tgatagtata atgtcaatgt
     4861 atgtaggttg ataagaagag tttgaatgat aactcattgc atttaaagat aaatggcatg
     4921 cgttatgatt taaagctctt ctttttttat attttataaa tttt
//
LOCUS       DAKSQE010000143         4835 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.144, whole genome shotgun sequence.
ACCESSION   DAKSQE010000143 DAKSQE010000000
VERSION     DAKSQE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.144"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            1..480
                     /locus_tag="O0V33_002413"
     CDS             1..480
                     /locus_tag="O0V33_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977011.1"
                     /translation="MSDYTKLSKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPQYLIYILFVLCTLTVLKLIIKPLWQYHCRFYQVDQLSVQYRTSFLIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVKIIEARTMSNLRGVESDV"
     gene            473..2050
                     /locus_tag="O0V33_002414"
     CDS             473..2050
                     /locus_tag="O0V33_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCX8977012.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFVIFQLKDFDYTNPE
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQMIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSST
                     EKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIAAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKMKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVPILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRH
                     FLIPSIIVIAMAGIGCYFWNQLLLLFTIITVVIIGLFVIKGYLYVKHAGFKMTDEQIT
                     IQNFVLLKRNIYYFKAQHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYASS
                     KDVQILKSWYLRGENDE"
     gene            2043..2534
                     /locus_tag="O0V33_002415"
     CDS             2043..2534
                     /locus_tag="O0V33_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCX8977013.1"
                     /translation="MSDYKFMSPHAKKVMRLSAVLFWIPIAFVLLIAFNILNWLFWEI
                     LDNYISIVSSVIIILLVAIFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHIENNK
                     NEE"
     gene            2538..2897
                     /locus_tag="O0V33_002416"
     CDS             2538..2897
                     /locus_tag="O0V33_002416"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCX8977014.1"
                     /translation="MIHGIGVDLIEIDRIKVLYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            2963..4111
                     /gene="alr"
                     /locus_tag="O0V33_002417"
     CDS             2963..4111
                     /gene="alr"
                     /locus_tag="O0V33_002417"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCX8977015.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTKTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYHRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVAAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            4196..4366
                     /gene="mazE"
                     /locus_tag="O0V33_002418"
     CDS             4196..4366
                     /gene="mazE"
                     /locus_tag="O0V33_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCX8977016.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            4363..4725
                     /locus_tag="O0V33_002419"
     CDS             4363..4725
                     /locus_tag="O0V33_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCX8977017.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVS
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVVHQKN"
BASE COUNT         1708 a          660 c          922 g         1545 t
ORIGIN      
        1 atgtcagatt atactaagtt atctaagagt cctaagtcag cattgttgta ttactacatt
       61 acgaatggct tagaatttat attgagtgta gctgtgtatg tcatttttta ttttatatgg
      121 ttaagatttg aatggcctca gtacttaatt tatattttat ttgttttatg tactttgact
      181 gttttaaaat taatcattaa gcctttatgg caatatcact gtcgctttta tcaagttgat
      241 caactcagtg tacaatatcg tacatcattt ttaatatata aagaagaaac gagtcgaatt
      301 gaacgtttac aatacttgtc gattaaatct aatccaatta gtaaagtgct taatttgtat
      361 aaagttggat ttatgactgc aggtcatact atttatttac cgatgatgtc tcatgacgat
      421 gttaaaataa tcgaagcgag aactatgtcg aatttgagag gggttgaaag tgatgtctga
      481 acctcaaaaa ttacatccga tttcatattt taatggcata atcaatgcta tcaaacaaaa
      541 catagtcgtc ttttttattt tcgttatctt tcaattgaaa gattttgatt atacgaatcc
      601 cgaatcatat ttatggatag gtattgtatt tgtattcttt ttaatatcat acatttctca
      661 aattgtcgga attatgaaca cgcgatattg gattgaggat aattatttca ttttaacaac
      721 aggtattttt aataaaaagc gtaaagaatt aaatataaaa cgtattcagt ccgttgatat
      781 gactcaaggg gtcgtcaatc aaatgatagg tggcgtggat ttacaaatca aaacgccaag
      841 tgatggtatt gtcttaagtg ttatttcgaa aaagcaaggt gaatatcttg aacgatatat
      901 tgatcaattg caaacagagt tgaagacaga agtggcgcaa aagtctagta ctgagaagaa
      961 tgacaactgt aatcatgaca ttgaaggtaa tgtggaagac atcgcagaag gtcgcttaga
     1021 tgaagtgcag aacactgata gtatgctaaa tcagaaaaat aaacgcattc ctatatatca
     1081 gatgaatttt aaagaatcac tatttatggc aatgaccagt ggcgccattg gtgtaacgtt
     1141 agcagcttta gtaccgattt acggcgctgt aagagaattg atcccatggt cgaagctaaa
     1201 tcatgaaatt agtcaatggg ttcaaattgt aagtatttca gtattaatta tcattttact
     1261 agtattaata gcggcatata ttattggaac gattataacg atgataagat tttatggctt
     1321 ttcggtgatg cttgaaaatg agcaacttaa aattgaatat ggactcttta acaagatgaa
     1381 agtgactgta ccaacgaaac gattgcaagc tgttgttgaa cgtcagtcat atatacgaag
     1441 attatttggc tacactgcaa tacattttat catcacaagt gattttgaaa ataaaaaaat
     1501 cgaagatgat gacaatgatt caggtagtgt gccgatattg ccgtttatta aacgagacaa
     1561 agcatatcaa attatccaga atctagtacc agatttatct tatcagcaag tcaatgaagg
     1621 tatgcctttg tcaggtttcc acagacactt tttaatacct agtattattg tcatagctat
     1681 ggcaggaatt ggctgttact tttggaatca attattattg ttatttacga ttataacagt
     1741 agtcattatt ggtttatttg taatcaaagg atatctttat gtcaaacatg cgggttttaa
     1801 aatgacagat gaacaaatta ctatccagaa tttcgtgcta ttgaaacgaa atatttatta
     1861 ttttaaagcg caacacatta taggatttga cacttggcaa catacattac ttaaacgcaa
     1921 caatttaaat aattttagtt ttattgttgc taaaggtgtg ggagcccaat cgattaactt
     1981 aaaatacgca agtagcaaag atgtgcaaat attgaagtcg tggtatttga gaggtgaaaa
     2041 tgatgagtga ttataagttt atgtcaccac atgctaaaaa agtgatgagg ttaagtgctg
     2101 tgctgttttg gataccaata gcgtttgtac ttttaatagc atttaatatt ttgaactggc
     2161 tattttggga gatattagat aactatatat ctattgttag tagtgtgatt ataatattac
     2221 ttgttgctat tttcacgctt gtcatagcgc cagtctatcg atataagcac tgcagatatg
     2281 catttgaaaa acaccatttg cgtgtacgaa acggcatttt atttttggat gaaaaagtaa
     2341 taccatattt tcgtattcaa aatattgata tcgatgttgg tcctataatg agacgctatc
     2401 gattagcaac tttgacactt tatacagcgg gtggtcaagc tgaaattgaa ttaatagata
     2461 gaagtgaagc acgtaggttg aaacgctggt taaataacga gagacatata gaaaacaata
     2521 aaaatgagga ataatcaatg atacatggaa ttggtgtaga tttaatcgaa atagatcgaa
     2581 taaaagtgtt atatagtaag caaccaaaat tggttgagcg gattttaact aaaaatgaac
     2641 agcacaaatt caacaatttc acacatgagc aacgtaaaat tgaattttta gctggcaggt
     2701 ttgctacaaa agaagcgttc agtaaagcat taggcacagg cttaggaaaa catgtcgcat
     2761 ttaatgatat agattgttac aacgacgaac ttggcaaacc aaagatcgat tacgaagggt
     2821 tcatcgtaca tgttagtatc tcacacactg agcattatgc gatgagccaa gttgttttag
     2881 aaaagtcagc attttaaatt tgttatacaa tgataattaa aatctagttt taaaacgtgt
     2941 gggaattaca ggaggaagta atatgtcaga taaatattat agatctgcgt atatgaatgt
     3001 agatttaaac gctgttgcat caaatttcaa agtattcagt acattgcatc caaataaaac
     3061 agtgatggct gtcgttaaag ccaatgccta tggactaggt agtgttaaag tagcacgtca
     3121 tttaatggaa aatggtgcca cattttttgc tgtagcaacg ttagatgaag cgatagaact
     3181 tagaatgcat gggattactg ctaaaatttt agtcttaggt gtgttaccag ctaaagatat
     3241 tgataaagcg atacaacacc gagttgcctt aacggttccg tctaaacagt ggttgaaaga
     3301 agcaattaaa aacatttctg gtgagcaaga gaaaaagtta tggttgcata ttaaattaga
     3361 tacaggaatg ggacgtttag gtattaaaga tactaaaacg tatcaagaag tgattgaaat
     3421 cattcaacaa tatgagcaac ttgtatttga aggggtgttt acacactttg cctgtgctga
     3481 cgaaccagga gatatgacaa ctgaacaata tcaccgtttt aaagatatgg tcaatgaagc
     3541 aattaaacct gaatatatac attgtcagaa ctcagcaggt tctctattaa tggattgcca
     3601 attctgtaat gcaataagac caggaatttc cctttatgga tattatccat cagagtatgt
     3661 acagcaaaaa gttaaagtac accttaaacc aagtgtgcaa ttaattgcta atgtagttca
     3721 aacaaagacg ctacaagcgg gtgagtctgt aagttatggt gcaacttata cagctactga
     3781 tccaacaaca atagcattgt tacctattgg atatgcagat ggctatttac gcattatgca
     3841 aggtagcttt gtaaatgtaa atggtcatca atgcgaagtt attggtcgcg tatgtatgga
     3901 tcagacaatt gtaaaagtgc cagatcaagt taaagctgga gattcggtga ttttaataga
     3961 taatcataga gaaagtccac agtcggtaga ggtggcagct gaaaagcaac atactattaa
     4021 ttatgaagtg ctttgtaact tgtcgagacg tttgccgcga atctatcatg atggtgatca
     4081 acgttttgta acaaatgaat tgttaaaata atatggtcag tgcatataaa atttgttatc
     4141 attagagtaa ttaaaggtca tttaataact tttggaatca attggaggtt ctcatatgtt
     4201 atcttttagt caaaatagaa gtcatagctt agaacaatct ttaaaagaag gatattcaca
     4261 aatggctgat ttaaatctct ccctagcgaa cgaagctttt ccgatagagt gtgaagcatg
     4321 cgattgcaac gaaacatatt tatcttctaa ttcaacgaat gaatgattag acgaggagat
     4381 gtttatttag cagatttatc accagtacag ggatctgaac aagggggagt cagacctgta
     4441 gtcataattc aaaatgatac tggtaataaa tatagtccta cagttattgt ttcggcaata
     4501 actggtagga ttaacaaagc gaaaataccg acacatgtag agattgaaaa gaaaaagtat
     4561 aaattggata aagactcagt tatattatta gagcaaattc gtacacttga taaaaaacga
     4621 ttgaaagaaa aactgacata cttatccgat gataaaatga aagaagtaga taatgcacta
     4681 atgattagtt tagggctgaa tgcagtagtt catcagaaaa attaggcgtc tattatatgt
     4741 atttttcaga gataaataaa atattgatat aaaagacaat aactttataa taattataac
     4801 tatttctaaa ttctgtacga agaaatttct tataa
//
LOCUS       DAKSQE010000144         4770 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.145, whole genome shotgun sequence.
ACCESSION   DAKSQE010000144 DAKSQE010000000
VERSION     DAKSQE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.145"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..430)
                     /locus_tag="O0V33_002420"
     CDS             complement(<1..430)
                     /locus_tag="O0V33_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCX8977018.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMTYPLHKSIHLLFFLPYRKSFKVHKLTKRKWLIFYNTYVNQPV
                     HKFYFCINLILPLIILSAMFVYLTISFPQYGHYFMFLLALN"
     gene            complement(1163..1528)
                     /locus_tag="O0V33_002421"
     CDS             complement(1163..1528)
                     /locus_tag="O0V33_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCX8977019.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            complement(1670..2092)
                     /locus_tag="O0V33_002422"
     CDS             complement(1670..2092)
                     /locus_tag="O0V33_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCX8977020.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            2226..2966
                     /locus_tag="O0V33_002423"
     CDS             2226..2966
                     /locus_tag="O0V33_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8977021.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDEAMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHNQTLDDIYIHVTQGGDVHA"
     gene            2959..4182
                     /locus_tag="O0V33_002424"
     CDS             2959..4182
                     /locus_tag="O0V33_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8977022.1"
                     /translation="MRNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFSLIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGVSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFIIFALTYYLLLAPKWYITFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGNKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLIVWLSYPLVTAIIGCLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEDKVIKGYEQFLYRLMFVICTVFALTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(4306..>4770)
                     /gene="traP"
                     /locus_tag="O0V33_002425"
     CDS             complement(4306..>4770)
                     /gene="traP"
                     /locus_tag="O0V33_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HCX8977023.1"
                     /translation="LNQIKINNPSHHLFQFSTADSSVIFEETEENTVLKSPSIYEVIK
                     EIGAFNEDHFYCAIFIPSTEDHVYQLEKKLISVDDNFKNFGGFKSYRLLRPVKGTTYK
                     IYFGFADRQTYEDFKNSDAFKDHFSKEALSHYFGSSGQHSSYFERYLYPIKE"
BASE COUNT         1639 a          687 c          784 g         1660 t
ORIGIN      
        1 aattcaatgc caataagaac ataaaataat gtccatattg cgggaatgaa attgttagat
       61 aaacgaacat tgcagataag ataattaacg gcaatattaa gttaatgcaa aaataaaatt
      121 tgtgtacagg ttgattgacg taggtattat agaatataag ccattttctt ttagtcaact
      181 tatgaacttt aaacgatttt ctatatggta agaaaaataa taaatgtata cttttatgta
      241 atggatacgt cataaataca agtaataaaa agatgagaaa atgtctatct gataacggcg
      301 tattagataa aaaatacatt acttcataac taactaaaaa cataatgatg gttgcaactg
      361 cactcataaa tgcaattcta ggcaaaccaa atcgtgcatt gatatcaatt tgtcttttac
      421 ataaaaacat atcttacgcc tcatctcttt tcaatataag ttagattata actaactcag
      481 gttcagtaat tcaatagtaa catatcatgt tagaagattt gttatatatg aagaaagggg
      541 cgacgtattg tttgatttga aacgcgaaag tgggattggt aaaattggct gattgactag
      601 cgtagaagta gaatgcagaa caaatttctg cgaacacata tttttgctaa taaagcatca
      661 cttatgaaaa aggcgcgtgt gacatgagta tttgaaaact tgaataatat aaaaaatgga
      721 tatggcaggg catatgcaaa agcatctttt aactaactaa gtcatacaaa tgctaaaaat
      781 atagtgatat gtaattataa taaataccag attaataatt tgtggaagta aggtatatgt
      841 aatgtgcata taaggttcgg ctcagtgaaa atataagtat gttgtttgtc gataatgatt
      901 taagtaaagc gtaatcaaag tgcaaggata acacaatgaa tcctaagcaa tattttcaat
      961 caattcataa atatgattta agggaaaatc actacaaaca acgtcttact gtaattggta
     1021 atcaaacatt cattcgagcc ataaaaaaga gatgacaaga aatatctaat ttctaatgtc
     1081 atctcgttta tttggcattt aattttaaaa aataaatatg ctgttactaa taaaaatagt
     1141 tgtgatcgag aacgtaaacg tgctacttag aaatttcgtt tccaatatcc tcgccacgat
     1201 tttgtaaatt ttcgatgtgt gactgtaaac gttcaatatt aggattgatg tcagatttga
     1261 agtttccaag taatgactta atttcaccac cgattgaact accaaattcc gcactttcgt
     1321 ttttaatttg aacaccatag tttaaaatgt cattaaaact ttgtttaatc gtttcaaatt
     1381 cacgttgtag ttctgatttt gaaccagtag gggcagtacg atcaatcgtg ttgttcttga
     1441 cacttttgtc gtcacgtcct tgaagtgcaa ctacaaaacc agctgctacg ccaacaccga
     1501 taccgaatag aatgcgtgat gctttcattt atattctcct cacttttaac atctgttatc
     1561 ttaataactc attttgttta aaattgctaa ccttgatgtt atcacttcaa acgcaattgt
     1621 tataatatta atcatattat acccgttcga gcttagatta agcatacatt taaaattgtg
     1681 cttgaatttg ttcagcaatt tgttgttttg catcgttatc taaaatatct tcatgtgttt
     1741 cccacttata accaaatcca tcaatatcat tttcatatcg aggaattaag tggaaatgaa
     1801 tatgaaatac cgattgatct gcaaactcac cattattttg aataatgttt aaaccatcag
     1861 gattaaatgc acgcttaata gcatttgcta ctttaggtaa tgctgcgccg atatgtttca
     1921 ttgtttcttc atcagtttca aagatattag cagaagcttt tttaggaatt aataacgtat
     1981 gtcctttagt aacttgtgat atatctaaaa aggcatagac ataatcgtct tcatatactt
     2041 taaagctagg aatttctcca gttaaaattt tgccgaaaat tgtttctgac atgatgacta
     2101 cactcctttt caacttgtat taccattcat tataacattg tcgctaaaaa ttcgatatta
     2161 ggatatgtgt atttaatatg agcattttat aatttttggt acaatataag tcatggaggt
     2221 gccttatgac agttaaagta gaacagctta caggtggata tggaaaacgc cctgtaatta
     2281 aagatataaa ttttgaatta aacaaaggcg aaatcgttgg acttattggc cttaatggcg
     2341 ctggtaagag tacaacgatt aaacatatgc taggattgct tacacctatg gaaggatctc
     2401 tatcaatttc agatatcaac attaatgatg acatagaggc ttatagaaga aagttatctt
     2461 atattccgga atcaccggtt atatacgaag aactcacatt agaggaacac attgagatga
     2521 cagcaatggc atatgatatt gatcgtgatg aagcgatgaa tcgagcaatg ccattattaa
     2581 agacattccg tttagaaaat gaattgaaag tttttccgag tcatttttct aaggggatga
     2641 aacaaaaagt catgattatt tgtgcgttta tcgttaatcc cgaattgtat attatagacg
     2701 aaccattcct tggcctagat ccattaggaa ttcaatccat gttagattta atggttgaaa
     2761 agaaaaacga aggtagaacc gttctaatga gtacacatat tttagcaaca gctgaacgtt
     2821 attgtgatcg ttttatcata ctagacgaag gtgaagtcgt tgcatttgga gatttagaag
     2881 cattgagaca acaaactggc ttacataatc aaacgttaga cgatatatat attcatgtga
     2941 cgcaaggtgg agatgtacat gcgtaaccaa gcgaccacat tatttaataa aagattacat
     3001 gcccttcgta aagagaaaaa ttattataat aagtttatat ttaatggtca tttcatggtc
     3061 tttttactta ttttgctggg tgcatttatt tttggatatg gtgaatggtt aaaacatatt
     3121 ccaacgaata tcaatttttc attaatagca gcagtgattg ttgcgttgac atccattttt
     3181 ccaatgcgac cactcctaaa agaagcggat aaaatctttt tattaccatt tgaaaaacac
     3241 atgtctcaat ttatgcgtca tgcgatttta tatagctact ttgcgcgcat tttaatccaa
     3301 ctcattattg ttattgtaat gttcccgttg ttttacaata ttaatcaaca taatgtggca
     3361 ttttatattt gttttggagt cagtgcatta atttttccat atgttggttt acgcttaaga
     3421 tggcaatggt atcaatcggg acttaaaaca tggcaagtta atttaatatc attcatcatc
     3481 tttgctctaa catattactt actattagct ccaaaatggt acatcacatt tgtgatggtt
     3541 gctttgcctg tgttaataga atttttagtt aaaaaatata aacctggctt tttgtatcca
     3601 tgggagaaaa tgattgctat cgaacatcga catcatatga attattataa atttgttaat
     3661 atgtttaccg atgtaaaaca tttgaaagaa tctgcagtca gaagaagtta tctagatata
     3721 cttttaccag ttccaaaggg taataaattt aactctaatg cgatgtattt attcttattt
     3781 attagaagct ttataagagg gagagacgca tttaatatta tctttaggtt agtaattatt
     3841 gcagttttac ttatagtttg gttatcatat ccattagtga cagcaattat tgggtgttta
     3901 tttgtatata ttatattgtt acaaatggca caattttatt cacaacaagc atatggctta
     3961 tggcctcaag tgtggcctgt acctgaagat aaggtaatca aagggtatga acagttttta
     4021 tatagattaa tgtttgttat ttgtactgtg tttgcattga catttattat aaaacatatg
     4081 acgttattct atgttgtact cattttctat attgtaggtt tattaacgat tagaagtatt
     4141 attaaaaagt taaaatatca ggaaacatta ttacgagact aaaaaacttc cattggcatg
     4201 tatgttgtaa aggtgcatgt aatgttgaaa gccaaatgat acggcgttca gattacatta
     4261 gcatctatac gttaacagca taaccaatgg aagttttctt cgaatctatt cttttattgg
     4321 gtataaatat ctttcaaaat aacttgaatg ttgtccactt gaaccaaagt aatgacttaa
     4381 tgcttctttt gaaaaatgat ctttaaaagc atcagaattt ttaaagtctt cataagtttg
     4441 tcgatcagca aagccaaagt aaattttgta tgttgtacct ttaacaggtc ttagcaatcg
     4501 ataactttta aagccaccaa agtttttgaa attgtcatca acactgatta atttcttttc
     4561 aagttgataa acatggtctt cagttgacgg aataaaaatt gcgcaataaa aatgatcttc
     4621 attgaatgcg cctatttctt taataacttc atatattgaa ggtgatttca gtacagtatt
     4681 ttcttcagtc tcttcaaaaa tcaccgatga atcagcagtt gaaaattgga ataaatgatg
     4741 agatggatta ttgattttta tctgatttaa
//
LOCUS       DAKSQE010000145         4725 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.146, whole genome shotgun sequence.
ACCESSION   DAKSQE010000145 DAKSQE010000000
VERSION     DAKSQE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.146"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            2..874
                     /gene="dprA"
                     /locus_tag="O0V33_002426"
     CDS             2..874
                     /gene="dprA"
                     /locus_tag="O0V33_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCX8977024.1"
                     /translation="MIRLFLLKLYWAHFSTKQIHQFLMAYPNVIKEGGRKKDSYLCEW
                     VNREENVHLLRKYYAFIKLYHNDIIKELQKLKVSYITYMDTEYPVLLKEIYQFPLLLF
                     YRGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
                     IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEIGLVISEYPPHTPIAKYRFPERNR
                     IISGLSKGVLITEAKEQSGSQITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAK
                     VVLNANDIFEDYYI"
     gene            1048..3123
                     /gene="topA"
                     /locus_tag="O0V33_002427"
     CDS             1048..3123
                     /gene="topA"
                     /locus_tag="O0V33_002427"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCX8977025.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            3279..4586
                     /gene="trmFO"
                     /locus_tag="O0V33_002428"
     CDS             3279..4586
                     /gene="trmFO"
                     /locus_tag="O0V33_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HCX8977026.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLSERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEAAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
BASE COUNT         1797 a          696 c          881 g         1351 t
ORIGIN      
        1 attgattaga ctatttttgc ttaagttata ctgggcacac ttttcgacta aacaaattca
       61 tcaattttta atggcatacc ctaatgtaat taaagagggg ggcagaaaaa aagatagtta
      121 tttatgtgaa tgggtgaata gggaagaaaa tgttcattta ttacgtaaat actatgcttt
      181 tataaaactt tatcataacg atattattaa agaactgcag aaattaaaag taagttacat
      241 tacatatatg gatactgaat acccagtgct attaaaagaa atatatcaat ttccattact
      301 tcttttctat agagggaaca tcaaattaat aaataatatg catcatttgg cagtagtagg
      361 tgcaagagat tctacaagtt atacccaaca gtctttagaa tttttattat caaatgataa
      421 aagcaaatat ttaacaattg tttccggcct tgctcaagga gctgatgcaa tggcacatca
      481 aatagcttta aaatacaatc tccctacaat agcagtttta gcctttggcc atcaaacaca
      541 ttatcccaaa agcacattag cattaagaaa taaaatagaa gaaataggtt tagttatatc
      601 cgaatatcca ccacatacac caattgctaa atatagattt cctgagcgca atagaattat
      661 cagcggtttg tcaaaagggg ttttaattac tgaggctaag gaacaaagtg gtagtcaaat
      721 cacgatagat tttgcattag agcaaaatag aaatgtttat gttttacctg gatctatgtt
      781 taatcctatg acaaaaggta atttattacg tatccaagaa ggtgctaagg tagtattaaa
      841 cgctaatgat atatttgaag actactatat ttaaaactaa aaataacgag ttgcttcata
      901 tttcttttgt taccctttgt aatttatttt ttaataaaat ctataaaaaa tagacaggga
      961 aaatgatttg tttagatata aaacgttgac aaaagcaaaa ttaagcgttt atcatttatc
     1021 tttagtaatt agattagcga gggggaaatg acattggcag ataatttagt cattgttgaa
     1081 tcgcctgcaa aagcaaaaac cattgaaaag tatttaggta agaaatataa agttatagct
     1141 tcaatgggac acgtcagaga cttaccaaga agtcaaatgg gtgtcgacac tgaagataat
     1201 tacgaaccaa aatatataac aatacgcgga aaaggtcctg ttgtaaaaga attgaaaaaa
     1261 catgcaaaaa aagcgaaaaa cgtctttctc gcaagtgacc ccgaccgtga aggtgaagca
     1321 attgcttggc atttatcaaa aattttagag cttgaagatt ctaaagaaaa tcgcgttgtt
     1381 ttcaacgaaa taactaaaga cgctgttaaa gaaagtttta aaaatcctag agaaattgaa
     1441 atgaacttag tcgatgcaca acaagcgcgt cgaatattag atagattagt tggctataac
     1501 atctcgccag ttctatggaa aaaagtaaaa aaagggttgt cagcgggtcg agttcaatct
     1561 gttgcacttc gtttagtcat tgaccgtgaa aatgaaatac gaaactttaa accagaagaa
     1621 tattggacta ttgaaggaga atttagatac aaaaaatcaa aattcaatgc taaattcctt
     1681 cattataaaa ataaaccttt taaattaaaa acgaaaaaag atgttgagaa aattacagct
     1741 gcattagatg gagatcaatt cgaaattaca aacgtgacta aaaaagaaaa aacgcgtaat
     1801 ccagcaaacc catttacaac ttctacatta caacaagagg cggcacgtaa attaaacttt
     1861 aaagctagaa aaacaatgat ggtcgcacaa caattatatg aaggtataga tttgaaaaaa
     1921 caaggtacga ttggtttaat aacatatatg agaaccgatt ctacacgtat ttcagatact
     1981 gccaaagctg aagcaaaaca gtatataact gataaatacg gtgaatctta cacttctaaa
     2041 cgtaaagcat cagggaaaca aggtgaccaa gatgcccatg aggctattag accttcaagt
     2101 actatgcgta cgccagatga tatgaagtca tttttaacga aagaccaata tcgattatac
     2161 aaattaattt gggaacgatt tgttgctagt caaatggctc cagcaatact tgatacagtc
     2221 tcattagaca taacacaagg tgacattaaa tttagagcga atggtcaaac aatcaagttt
     2281 aaaggattta tgacacttta tgtagaaact aaagatgata gtgatagcga aaaggaaaat
     2341 aaactgccta aattagagca aggtgataaa gtcacagcaa ctcaaattga accagctcaa
     2401 cactatacac aaccacctcc aagatatact gaggcgagat tagtaaaaac actagaagaa
     2461 ttgaaaattg ggcgaccatc aacttatgcc ccgacaatag atacgattca aaagcgtaac
     2521 tatgtcaaat tagaaagtaa gcgttttgtt cctactgagt tgggagaaat agttcatgaa
     2581 caagtgaaag aatacttccc agagattatt gatgtggaat tcacagtgaa tatggaaacg
     2641 ttacttgata agattgcaga aggcgacatt acatggagaa aagtaatcga cggtttcttt
     2701 agtagcttta aacaagatgt tgaacgtgct gaagaagaga tggaaaagat tgaaatcaaa
     2761 gatgagccag ccggtgaaga ctgtgaagtt tgtggttctc ctatggttat aaaaatgggg
     2821 cgctatggta agtttatggc ttgctcaaac ttcccggatt gtcgtaatac aaaagcgata
     2881 gttaagtcta ttggtgttaa atgtccaaaa tgtaatgatg gtgacgtcgt agaaagaaaa
     2941 tctaaaaaga atcgtgtctt ttatggatgt tcgaaatatc ctgaatgcga ctttatctct
     3001 tgggataagc cgattggaag agattgtcca aaatgtaacc aatatcttgt tgaaaataaa
     3061 aaaggcaaga caacacaagt aatatgttca aattgcgatt ataaagaggc agcgcagaaa
     3121 taatattttt atttcctaga gacattttaa gattgttaaa tagaatcatt agtgaatctt
     3181 attttaaaga tagtaatgga ttaatctaaa taagtgcgga taatattaac ataacaacat
     3241 aattaaaaga cataaatgac aataaaagga gtatagaaat gactcaaact gtaaatgtaa
     3301 taggtgctgg tcttgccggt tcagaagcgg catatcaatt atctgaaaga ggaattaaag
     3361 ttaatctaat agagatgaga cctgttaaac aaacaccagc gcaccatact gataaatttg
     3421 cggaacttgt atgttccaat tcattacgcg gaaatgcttt aactaatggt gtgggtgttt
     3481 taaaagaaga aatgagaaga ttgaattcta taattattga agcggctgat aaggcacgag
     3541 ttccagctgg tggtgcatta gcagttgata gacacgattt ttcaggttat attactgaaa
     3601 cacttaaaaa tcatgaaaat atcacagtta ttaatgaaga aattaatgcc attccagatg
     3661 gatacacaat tatcgcaaca ggaccactta ctacagaaac ccttgcacaa gaaatagtgg
     3721 acattactgg taaagatcaa ctttatttct atgatgcggc tgctccaatt attgaaaaag
     3781 aatctattga tatggataaa gtttacttaa agtcccgtta tgataaaggt gaagctgcat
     3841 atttaaactg tcctatgact gaggatgaat ttaatcgctt ttatgatgca gtattagaag
     3901 ctgaagctgc gcctgtaaat tcatttgaaa aagaaaaata tttcgagggt tgtatgcctt
     3961 ttgaagtaat ggcagaacgc ggacgcaaga cattactatt tggaccaatg aaaccagtag
     4021 gattagaaga tccaaagact gggaaacgtc cttatgcggt ggttcaatta agacaagatg
     4081 acgctgctgg cacactctac aatattgtcg gcttccaaac acatttaaaa tggggagctc
     4141 aaaaagaagt cattaaatta attccaggtt tagagaatgt tgatattgtc agatatggtg
     4201 tgatgcatag aaataccttc attaactcac cggacgtatt aaacgagaaa tatgaattga
     4261 tttcacaacc aaacatacag tttgcgggcc aaatgactgg tgttgaaggt tatgtagaaa
     4321 gcgcagctag cggcttagtt gcaggcatca atcttgcgca taaaatatta ggcaaaggtg
     4381 aggtagtatt tccgagagaa actatgattg gaagtatggc ttactatatt tctcatgcta
     4441 aaaacaataa gaatttccaa cctatgaatg ctaacttcgg gttattacca tctttagaaa
     4501 ctagaattaa agataaaaaa gaacgctatg aagcacaagc taatagagct ttggattact
     4561 tagaaaattt caaaaaaact ttataaaata gttagaaaga ctagatatgc tattcattct
     4621 taagtcatca acgagtaagt aatgactttc taaatggaaa atacttatcc tagtcttttt
     4681 aattttggaa ttgttacgta tttctgacaa tttagaattc gtatt
//
LOCUS       DAKSQE010000146         4663 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.147, whole genome shotgun sequence.
ACCESSION   DAKSQE010000146 DAKSQE010000000
VERSION     DAKSQE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.147"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            48..1391
                     /gene="brnQ"
                     /locus_tag="O0V33_002429"
     CDS             48..1391
                     /gene="brnQ"
                     /locus_tag="O0V33_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCX8977027.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
                     KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGI
                     TKTNQIFKQTLTAGLIAAIALIFIYISLGYIGNHMPVSDMKLNELKSHDRNIGTYLLT
                     TMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMS
                     FIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRITQQIPVIIVFILSI
                     FSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
     gene            complement(1459..2595)
                     /locus_tag="O0V33_002430"
     CDS             complement(1459..2595)
                     /locus_tag="O0V33_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCX8977028.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLMSKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQQVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGPNKQS"
     gene            complement(2627..3256)
                     /locus_tag="O0V33_002431"
     CDS             complement(2627..3256)
                     /locus_tag="O0V33_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCX8977029.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGIIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            complement(3275..3544)
                     /locus_tag="O0V33_002432"
     CDS             complement(3275..3544)
                     /locus_tag="O0V33_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCX8977030.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            3706..4014
                     /locus_tag="O0V33_002433"
     CDS             3706..4014
                     /locus_tag="O0V33_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCX8977031.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCEEDLQIFYSFIVLQNNEVYYKLPIID
                     "
     gene            4185..4385
                     /gene="cspA"
                     /locus_tag="O0V33_002434"
     CDS             4185..4385
                     /gene="cspA"
                     /locus_tag="O0V33_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HCX8977032.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
     gene            4582..>4663
                     /locus_tag="O0V33_002435"
     CDS             4582..>4663
                     /locus_tag="O0V33_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSA protein"
                     /protein_id="HCX8977033.1"
                     /translation="MKYLILSLVANLLVFGVLSAIGLNINI"
BASE COUNT         1445 a          700 c          751 g         1767 t
ORIGIN      
        1 atattaactt gtaaattatt tcgtgtttta aaaaaaggag taactctatg aataaaaata
       61 catgggtcat tgggtttacg ctcttcgcta tgttttttgg cgcaggcaat ctaatttttc
      121 ctcctaatct cggattggat agtggtcaat ttttctggcc cgccatttta gcgtttgttc
      181 taactgggat tggtttacca ttattaggtg tgattgtagg tgcacttgat aaagaaggat
      241 atattggcgc attaaataaa atttcaccta aattttcaat attgttctta atcatcattt
      301 atttaactat aggaccactt ttcgcaatac ctagaactgc atctacatct tttgaaatga
      361 caattacacc aattatacat agtaatagta gtatcgcttt atttatattt acgattatct
      421 acttcatagt cgttttgtat atttgtttaa atccatctaa gttaatcgat cgtattggtt
      481 cattattaac accattatta ttgattacta ttttagcgat gattattaaa ggatacttag
      541 actttagcgg taatagtgct ggaaagggca atgaagcact atatcattct aatttttcaa
      601 gttttgctga aggctttaca caaggctatt taacaatgga tgccattgca gcaattgctt
      661 tttcaatgat tgttgttaat gcagtaaaac taacaggtat tactaaaaca aatcaaatat
      721 tcaaacaaac tttgactgct ggtttaattg cagcaattgc tttaattttc atatatattt
      781 cattaggtta tattggtaat catatgccag taagtgacat gaaactaaat gaattgaaat
      841 cacatgaccg taacattggg acatatttat taacgacaat ggcttcaaca ggatttggtt
      901 cattcggaaa gtatttattg ggcatcattg tggcgctggc atgtctaact acagcatgtg
      961 ggcttattgt tgcagtttct gaatatttcc atagaatcgt acctaaagta tcatacaaag
     1021 catttgtatt agttttcatt ttaatgagtt ttattattgc taaccaaggt ttaaatgctg
     1081 ttatctcaat gtcaattccg gtattaagca ttgtataccc agtagcaata actgttgtat
     1141 tattaatttt aattgccaaa ttcataccga caaaacgcat tacacaacaa attccagtta
     1201 ttatcgtatt tatattgtcg attttcagtg ttattagtaa gttaggttgg ctgaaaatta
     1261 actttataga atcattgcct ctaagagcgt attctttaga gtggttccca gtagcaatta
     1321 ttgcaacgat attaggctat ctagtcggca tatttgtaaa acaagatcca attaaatatc
     1381 aacaggaata acgaaaaaag gaagcatctc caatttaatc gtaggatgct tccttttttg
     1441 ttatatctaa ttcgtaattt atgattgttt attcggtcct ttgatgttca ctaaatgtga
     1501 ctttaaatct tgttctaatt gttgtaattc tttttcagct aattgtcgtt cttcgcgacc
     1561 gtgttgttga ataattaatg tttcttcaat tgtctcaata atgttacgct gtgtacgttt
     1621 caatgtatca aggtcaacaa tgccacgctc attttctgtt gcagtttcaa tcgcattttg
     1681 tttcaacatt tcagcatttg ctgttaataa atcattagtt gtatcagtga cagctcgttg
     1741 tgcagcaact gcattacgct gtctcattaa tgtaagcgca atggccattt gatttttcca
     1801 tagtggaata cttgtcaaaa ttgaactttg tatcttctcg gcaagggctt gattaacatt
     1861 ttgaatcata cgaatttgtg gcgcagtttg caacgctatt tgtcttgaaa gctgtaagtc
     1921 atatatgcgt ttatctagtc tatctataaa ttgctgcata tctgcaactt gttgaatatc
     1981 catttgatta gtggattgct gcgcttgctg ttgcaattgt ggtagctttt cattttctaa
     2041 ttgcaacttt ttttgctgtg cagcaatgat atgcaatgat aagtcatcaa agtattgttt
     2101 gtttttatca tatagcgtat ctaataattc aatatctctt gttaaatgtg tttgatgttt
     2161 ctgcagttga atcgttatgc gatcgacttg agcactaact gattgcattc ttgaaaagat
     2221 ttcattgata gacgactttg ctctgctaaa aattcttttt aacatagatg gtttatcagt
     2281 atttaactca tttggattaa ctgactttag ttttgacatt agatctgaca aagtatctcc
     2341 aataggacca acatctttac tttgtacctc atccaacatt tgatgtgaaa attgagacat
     2401 ttgtttctgt aaatcagaac caaacgctaa taaaccttca ttgtctaaag ggttaatttg
     2461 tttactgatt gtgtctacct gtttttgttg ttcaattgtt aacttttctt tttgaatagt
     2521 agtattcgat aattctttat cgcttataaa atcatctagt ggatggcttt ctttgaaact
     2581 tttattttca gtcatcaatc atctctccaa tagttgctat tttacatcaa tcttgatgat
     2641 gcttttgatg taacttattt aattccattt caatatctaa acgttcataa tcatcttcgt
     2701 tgagacgctt taaatcagcg attaatgttc gtttgacctc atccaaagta attcgtgttt
     2761 gttctaattt ttgctgttca ttaattgatt ttttgggcat ttttgctaga cgtgtatatg
     2821 catcaaccaa atttaaagca ttatcaatat gagaataaaa aaagccctca actttataaa
     2881 atgatgcagg tctctgtcta actgtcgtat aaatagaacg tgaaatttgg tatatatcat
     2941 taatttgcct aaaatcttta attgatctta tattgacata cgtttttaaa atacctctaa
     3001 gtttttggtg tgtatgattt aactgatttc gaatatagcg atagtctttt ctagtcaaac
     3061 caatttcgtt taaatatttg cgtgaagtga gtttttgtat cggtaggtat gtcattaaaa
     3121 agccaacaat accaatagac atatcaatta aaaaagatac atcaagtgca attatcccaa
     3181 atatgcttgt taaaaacgct acaggaattc ccactaacac cccaaatata tgagaaatat
     3241 tatatctcac tgtcatcttc ctttatttag cattttatat tgatcgaaaa tccgataatt
     3301 tttgatttag ttctaactct tcaagttgat ggctcgttac atttgatgct ggtgaggcac
     3361 cttcaattac accttgaata aatctctcta tatctgcgtc atccccttgt gcatatatct
     3421 ctacatagtc atctacattt tgaacagtac cgacaatgtt atagttcatt gcaatgcgtt
     3481 gtgtaaaata tctaaatccg acgccttgaa cgcgtccgaa tacttgtaaa tgtatatgtc
     3541 tcatttttac cacctcataa tgttattata cgtagtttta cttaaaaaaa ctaataatta
     3601 ctatagttaa tactttgttt gtttcaagtc gtcaaacttg attttcagag gataaaggta
     3661 taaaaataag tatagagttt tgaagtatgg aaggggtctt taataatgtg gacagttacc
     3721 aaaattagag ccgattatga gggatggtgg ttattcagtg actggccaga aaacattgtt
     3781 gaaaaatatc aatatcaaga ttttgatgac atgtttaagc actatcaaca attgattaat
     3841 caatgtaaag ttcagttcga taactatgtc acaggcaaat ataatattta tgcattttat
     3901 aataattgtg acatgaacta ttgcgaagat tgtgaagagg atttacaaat attttatagt
     3961 ttcattgttt tacaaaataa tgaagtatat tataaactac caataattga ttaaatgaat
     4021 attttttatt attgtattgc aatttggtaa taactgcact ataatacaag ttaagcagat
     4081 gattattcca tattgcaaag aatatttgag tgaaatttgc atgtattttt tgtaatatgc
     4141 gaataagcat attgaatgaa ttttagtctt ggaggtttca gattatgaaa caaggtacag
     4201 ttaaatggtt taacgctgaa aaaggattcg gctttatcga agttgaagga gaaaatgacg
     4261 tattcgtaca tttttcagca attaaccaag atggttacaa atcattagaa gaaggtcaag
     4321 ctgttgagtt tgaagtagtt gaaggcgacc gcggtccaca agctgcaaac gttgttaaac
     4381 tataattctt agatttgaat cattgatttt aactaacacc ttaccaagaa gtaaggtgtt
     4441 tttatatgct tattacaaat acttaacgcg atgagtttct caaccaaatt gagaactctt
     4501 tttttatatc accaacattg tttgcaatag aaaaatatat ttatataata taattatggt
     4561 gtacaacgac gaggtgattt tatgaaatat cttatcctta gtttagttgc taatttatta
     4621 gttttcggtg tattatctgc aattggttta aatattaata ttt
//
LOCUS       DAKSQE010000147         4646 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.148, whole genome shotgun sequence.
ACCESSION   DAKSQE010000147 DAKSQE010000000
VERSION     DAKSQE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.148"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(24..112)
                     /locus_tag="O0V33_002436"
     tRNA            complement(24..112)
                     /locus_tag="O0V33_002436"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(75..77),aa:Ser,seq:gct)
     gene            complement(115..189)
                     /locus_tag="O0V33_002437"
     tRNA            complement(115..189)
                     /locus_tag="O0V33_002437"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(155..157),aa:Asn,seq:gtt)
     gene            complement(331..900)
                     /locus_tag="O0V33_002438"
     CDS             complement(331..900)
                     /locus_tag="O0V33_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCX8977034.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
     gene            1110..1328
                     /locus_tag="O0V33_002439"
     CDS             1110..1328
                     /locus_tag="O0V33_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HCX8977035.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYMAEYKNLEAFLGE"
     gene            complement(1409..2395)
                     /locus_tag="O0V33_002440"
     CDS             complement(1409..2395)
                     /locus_tag="O0V33_002440"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCX8977036.1"
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIV
                     GKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKF
                     TEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
                     NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLT
                     DEDWENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIF
                     GDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            2594..2770
                     /locus_tag="O0V33_002441"
     CDS             2594..2770
                     /locus_tag="O0V33_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HCX8977037.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
     gene            2785..3387
                     /locus_tag="O0V33_002442"
     CDS             2785..3387
                     /locus_tag="O0V33_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCX8977038.1"
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAG
                     ISYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVN
                     WKWQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWVAILITAICSSSLFIYLDNWWI
                     VTFIFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     gene            3658..3765
                     /locus_tag="O0V33_002443"
     CDS             3658..3765
                     /locus_tag="O0V33_002443"
                     /inference="COORDINATES: protein motif:HMM:NF028245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCX8977039.1"
                     /translation="MISIANALHLMLSFGMFIVTFIGVVVAIINLNNKK"
     gene            3935..4036
                     /locus_tag="O0V33_002444"
     CDS             3935..4036
                     /locus_tag="O0V33_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977040.1"
                     /translation="MKFAANKLTLTIFRKLCINNEKEIWIKYEEVFL"
     gene            4046..4318
                     /locus_tag="O0V33_002445"
     CDS             4046..4318
                     /locus_tag="O0V33_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HCX8977041.1"
                     /translation="MFGTILYLSLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYY
                     HAHKGHGATAIGKYRSFSGYTRASVKAKASATKHNLRVNRAYYNIY"
     gene            4362..>4646
                     /locus_tag="O0V33_002446"
     CDS             4362..>4646
                     /locus_tag="O0V33_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCX8977042.1"
                     /translation="MKWFKLILDVTIFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRINQFAKNENVAIYKSTLNYTNKNVDKDIYVFNKSKATTI"
BASE COUNT         1588 a          678 c          713 g         1667 t
ORIGIN      
        1 gtatatacca tttaagcaag taacggaaac ggagggattc gaaccctcgc gccgctttcg
       61 cgacctacac ccttagcagg ggcgcctctt cagccaactt gagtacgttt ccaatggctc
      121 cacaggtagg actcgaacct acgaccgatc ggttaacagc cgatagctct accactgagc
      181 tactgtggat taatttgtat gtgtttgaat atcaagcaca aatattatta taacaagatt
      241 aagttaattt gcaatagata ccttaaaaaa atatgcagaa catttacact atatttataa
      301 aacattccgc atattttaaa aataaacaat ttatttatct tcaaataaag aatagcgtgt
      361 caatgcttca aacacattca atgtggcttt attataatct attggttggt cgggattttt
      421 tgatatcatg cgagattgtt tatctaccat gtaatagtaa aaaatggaat tgttatattg
      481 gtgccataaa ctgtggtatg aaacatgaag aatgattgat tcattattaa taaatgtcac
      541 tttacattta cgatttttta attctttaat actttcgata taatgcatat ttaaccaaat
      601 attttcattt tgtctgtcag aatgtgttgg gaaaaaataa gttggaaata atggtgttag
      661 taaaataggt ggtttactag aaatgccagt aatgcggttt gtctctgctt tcttgccaag
      721 ataagtatta ccataaaatt tgcaagagcg ttcaatgatt ttctggactt taaaaggatt
      781 ttgaatacgc gttttgtcaa accgaattat ttcactacca tttctttgat cgtcatcatc
      841 aaatgctggt cgaataacca tgtctccttt gcgtatcaca taaatatttt gagaatacat
      901 aagcaaaacc tcgctttatt aagttttaaa ccattaatat aaaaatgcaa ttaatgatat
      961 aaagatattt tataataaaa ttctgagttg tcaagttatt attttggtga aaatgtacaa
     1021 taatttttaa ctaatagtca gaatattcat tgatttcaaa attagaaaaa tgtatactta
     1081 ttataaatta aagaaaggta gtggtttcta tgaaatacaa tactaatgtt aaacatacaa
     1141 ctttagaagc gtttgtcaca accgtcaatg atttgggtat tgaattaatt atcaatgaag
     1201 cacttcgaga ggtaagaaaa cgacagctca tagaacttat agatgacgca ctcgtcaata
     1261 aagatgaagc agcatttaat caatatatgg cagaatacaa aaatttggag gcatttctcg
     1321 gtgaataaca ttgattcgaa ttccataacg caatcataaa aggactattc ttaacatctc
     1381 atacatgtaa gaatagtcct ttaatcgttt atgacattaa tctaattaat tcatgtctat
     1441 ctatgtcacc gaaatagtga tataaatcat attctgatag tgcttcttct atgtgctcaa
     1501 agtcgtgtag acagccaact aatgcatttt caagatcagt gacatctccg acaccaaaga
     1561 aatcaccgaa aatttttgca tgttcgattt tacctcgttt aacatcaaac ttgatttgta
     1621 caaatccttt ttcaaatttt tcctcgcgtt caaagttata tttagggttt cttccataat
     1681 tccattccca agttctatat ttgtcgttac ttaacttttc aatattttcc caatcttcat
     1741 ccgttaattt atattcttct acttcagttt ctccaaagat agttttcaag atgatttttt
     1801 taaattcttc aatttctaaa gggtcattta aaaattcttg aatgtttgct actcgtttac
     1861 gaacagattt aatacctttt gatttaatct tagctggatt aacttttagt gcattctgaa
     1921 cttcgtctaa atcactattt aacatcaacg taccatgact aaacattcta tttttaactt
     1981 taaccatggc atttcctgag attttcgctt gtccaacttg aatatcgtta cgaccagtta
     2041 attcagcatt tacgcctaaa gattgtaatg cttgaacaat tggttcagtg aatttttgga
     2101 aattgtggaa actgttacca tcatcatctg ttataaaact aaagtttaaa ttgccagtat
     2161 catgataaac agcgccacca ccagaaattc ttcttactac atcgatgtta tgagcatcta
     2221 tatatgtctg atttacttcc tctatcgtat tttgattctt tccaacaatg atagatggtc
     2281 tatttatgta aaataaaaag taactttctt ctgctggtaa attttttaaa acatattctt
     2341 ccattgctaa gtttaaagtt ggatctgtaa tattattatt actaatgaat ttcattacaa
     2401 tctctccctt atatctatat atattctcta cttatttatg ccttaacttt gctgaaaatg
     2461 ccaattatat actcacaaat cgttctaaat atcggcttaa agttacattt tattattttt
     2521 gattgttaat agtttatttg ttaagtgaat tttagtacaa tataatttag ttatgattta
     2581 aggaggactt aaaatgactg ttgcagaagt gggtaacatt gttgagttta tggatggatt
     2641 aagaggtcgt gttgaaaaaa tcaacgataa ctctgttatt gttgacttaa caattatgga
     2701 aaattttaat gaccttgatt taccggaaaa aactgttatc aatcataaac gatataagat
     2761 tgttgaataa gaaggtaagt tataatgaat aaaatctcga aggctttaac ttggtttatt
     2821 ataagtttca ttatatttca tctcatatta tttattatgt ggggcgaaca ccaagaatac
     2881 tggtatttat atacaggtat aatgctaatt gctggtatca gttatgtgtt ttatcaaaga
     2941 gatattgaat ctaagcggtt gcttacatca attggtgttg gtattattac ggcaattatt
     3001 ttaattatgc ttcaactttt attctcactt ataacttcta atttaagtta tagttcatta
     3061 attaaagaat tagcaagaac aggtgtcaat tggaagtggc aaatgttagt aactttactt
     3121 tttgtcattc catgtcacga gttatatatg agaactgttt tacaaaaaga attaacacat
     3181 ttttcaatac cgaaatgggt agctatatta ataactgcaa tttgttctag ttcattattt
     3241 atttatttag ataattggtg gatagtaaca tttatatttg ttgcacaagt gatattatca
     3301 ttaagttatg agtatactcg acgtatagca acaacatccg ttgcacaaat tgtagctatt
     3361 atattgttat taatttttaa tgcttaaata agcttttctt agacgtctgt gttctaagaa
     3421 aagctttttt gctttagaat agcaatacga agattcgagg catgcttcat tatagtgtac
     3481 acgtttttaa cggcaagctg ttattattga gattaggttt tagtcagttt ttattactaa
     3541 atattttata aaaacagtat aatatttttc gagcaagttt aattgatggt ggctatctga
     3601 gtaaaggggg gtggtgccta tggcattact taacactttt gaaaggaaaa gcctattgtg
     3661 atatctattg caaacgcatt acatttaatg ttaagtttcg gtatgtttat cgtcactttc
     3721 attggtgtag tagtcgcaat aattaattta aacaataaaa aataaccatc atttcaaaac
     3781 tttgacaaag aatgatggtt aaagcaaatc tcatttagtc accgtctttt taacgggttc
     3841 aataggggac atgttcctgc atgtcgtcta tttatgcact gcgcaattta ataataatac
     3901 aattatactt tgatgtccat ttaatataaa gtggttgaaa tttgcagcaa ataaattaac
     3961 cttgactatt ttcagaaaat tatgtataaa taacgaaaaa gagatatgga ttaaatatga
     4021 agaggtgttt ttatagaaga ataaaatttt tggcactata ttatatttat ctttagcact
     4081 tggattatca acagcagctt atgcatctac agaatacgct gaaggaggca cttggagtca
     4141 cggtgtcggc agtaagtatg tttggtcgta ttattatcat gctcataaag gtcatggtgc
     4201 aacagctatc ggaaaatata gatcatttag tggttataca agagccagtg taaaagcaaa
     4261 agcatcagct actaaacata atttgcgggt caatagagcg tattataaca tttattaaat
     4321 actaaaaaat catagaaagt aaacaacgca agaggtacaa catgaaatgg ttcaaattaa
     4381 tacttgatgt tacaatcttc attctgattg ctatattgtt atttgtttat acatataaag
     4441 aaaacgaaga aatattgcct gatactaaat accctatagc ggtaactgac tggaataaaa
     4501 aatatagtaa aaatgagatt tataaacgta taaatcaatt cgctaaaaat gagaacgtag
     4561 caatctataa atcaacttta aattatacaa acaaaaacgt agataaagat atatatgtat
     4621 ttaataaatc aaaagcaaca actatc
//
LOCUS       DAKSQE010000148         4585 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.149, whole genome shotgun sequence.
ACCESSION   DAKSQE010000148 DAKSQE010000000
VERSION     DAKSQE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.149"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            224..2746
                     /gene="mprF"
                     /locus_tag="O0V33_002447"
     CDS             224..2746
                     /gene="mprF"
                     /locus_tag="O0V33_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCX8977043.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVVITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILN
                     KITWVRWVLYAVSLFLPLFIIYSMVRPPDKNNRYVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDVHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYLLLAIHTSACLLLLLNVVGIYK
                     QSRRAIIYAMISIILIIVATLFTYASYILITWLVIIFALLIVAFRRARRLKRPIRMRN
                     LVAMLLFSIFILYINHIFIAGTFYALDVYTIEMHTSVLKYYFWITILIIAIIVGAIAW
                     LFDYQFSKVRISSNIEECEEIIDQYGGNYLSHLIYSGDKQFFTNEDKNAFLMYRYKAS
                     SLVVLGDPIGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDN
                     RQEMHFSVGQFNETYLSKAPIGVMRNENNEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
     gene            complement(2949..3458)
                     /gene="msrA"
                     /locus_tag="O0V33_002448"
     CDS             complement(2949..3458)
                     /gene="msrA"
                     /locus_tag="O0V33_002448"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCX8977044.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            3595..4578
                     /locus_tag="O0V33_002449"
     CDS             3595..4578
                     /locus_tag="O0V33_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCX8977045.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
BASE COUNT         1480 a          621 c          807 g         1677 t
ORIGIN      
        1 ttttcataag tattgattca tcattttctt tatgttaaat atgaatcagt atagataacc
       61 atattgttct gtttgagata gaaatatttt ctatttactt cttaatacag atgtcaatat
      121 gacaaaagtg ttatataaat caaaggtaaa tgatatgcta tttttatggt ttatatgata
      181 tattttgatt tataacagaa ataattagaa ttgatgtgaa aaaatgaatc aggaagttaa
      241 aaacaaaata ttttcaatct taaaaattac gtttgctaca gctttattta tttttgtagt
      301 aatcacgttg tatcgagagt tatctgggat taactttaaa gatactttgg ttgaatttag
      361 taaaatcaac cgtatgtccc ttgtcttatt atttattggt ggtggggcat cgcttgttat
      421 tctctcaatg tatgatgtga ttttatctag agctttaaaa atggatattt cattaggaaa
      481 agttttaaga gtaagttata tcattaatgc attaaatgcg attgtaggct ttggtggctt
      541 tattggtgcc ggtgtaagag caatggttta caaaaactat acgcatgata aaaagaaatt
      601 ggttcacttt atttcattaa tactgatttc aatgttgaca ggtttaagtt tgttatcatt
      661 gttaatcgta ttccatgttt ttgatgcatc attaatttta aataaaatta cgtgggtgag
      721 atgggtactt tatgctgtgt cgttgttctt accattattt attatatatt caatggttag
      781 accgcccgat aaaaacaatc gttatgtagg cttgtattgt acattagtgt catgtgtgga
      841 atggttagcc gctgcagttg tattatactt ctgtggtgtc atagttgacg tccatgtctc
      901 atttatgtca tttattgcaa tattcattat tgctgcatta tctggattag tcagctttat
      961 tccaggtggt tttggtgcct tcgacttagt tgttttatta ggctttaaaa cgttaggtgt
     1021 acctgaagaa aaagtattat taatgctact actttatcgc tttgcgtact atttcgtacc
     1081 agtgattatt gcattaatcc tttcatcatt tgagtttggt acttcagcta agaaatatat
     1141 tgaaggatct aaatatttca ttccggctaa agatgtgaca tcgtttttaa tgtcgtatca
     1201 aaaagatatt attgctaaaa taccatcatt atcattggca attttagtat tctttacaag
     1261 tatgattttc tttgtaaata atttgacgat tgtatacgat gctttatatg atggcaatca
     1321 tttaacgtat tatcttttat tggcaataca tacaagtgcc tgtttattac ttttacttaa
     1381 tgttgtaggt atttataaac aaagtagacg tgccattatt tatgcaatga tttcgattat
     1441 cttaattatt gtcgcgacgt tattcactta tgcgtcatac attttaataa cttggttagt
     1501 tattatcttt gctttactta ttgttgcttt ccgtagagca cgtaggttaa aacgtccaat
     1561 tcgaatgaga aatctagtcg caatgctttt gttcagcata tttatactct acatcaacca
     1621 tatatttata gctggaacat tttatgcttt agatgtttat acaatcgaaa tgcatacgtc
     1681 tgtacttaaa tattatttct ggattaccat tttaattatc gcaattattg ttggtgcaat
     1741 tgcgtggtta ttcgattatc aatttagtaa agtgcgtata tcttctaata ttgaagagtg
     1801 cgaagagatt attgatcaat atggtgggaa ttatttaagt catttaattt atagtggtga
     1861 taaacagttc tttactaatg aagataaaaa tgcattttta atgtatcgct ataaagcaag
     1921 ttcgttagta gttcttggcg atccaatcgg tgatgaaaat gcctttgatg aattgttaga
     1981 agcattctat aattacgctg agtatttagg ctatgatgtt atattctatc aagttacaga
     2041 tcaacacatg cctttatatc ataatttcgg caaccaattt ttcaaattag gtgaagaagc
     2101 aattattgat ttaacgcaat tttcaacttc aggtaaaaaa cgccgtggat ttagagcgac
     2161 tttaaacaaa ttcgatgaac ttaatatttc attcgaaatt attgaaccac cgttttcaac
     2221 tgaatttata aatgaacttc aacatgtcag tgatttgtgg ttagataatc gtcaggaaat
     2281 gcatttctct gttggtcaat ttaatgaaac atatttatct aaagcgccaa ttggtgtgat
     2341 gcgcaatgaa aataatgaag taattgcatt ttgtagttta atgccaacat actttaatga
     2401 tgccatttca gtcgatttaa ttagatggtt gccagagtta gatttaccgt taatggatgg
     2461 attatacttg cacatgttac tttggagtaa agaacaaggt tatacaaaat ttaatatggg
     2521 tatggcaacg ttatcgaacg ttggtcaatt gcattattca tatttaagag aaagacttgc
     2581 aggtcgtgtc tttgaacatt tcaacggtct atatcgtttc caaggattac gtcgttataa
     2641 atctaaatat aatccgaatt gggaaccacg ctttttagtt tatcgtaaag ataattcgct
     2701 ttgggaatca ctttctaaag taatgcgtgt aatacgtcac aaataattaa aatccaagtg
     2761 ctaataggta tacagttatg cctgaagcgc ttggattttt tggtttctat aaagaatgat
     2821 ttataaaaaa cggcacagat aagaatattt aagtttccta tctgtaccgt attttaatat
     2881 tgaaatgaac ttaagttaag tcgcctaact tttattatca gtccaatcag tttcatcaag
     2941 tgtaaatgtt attgtttatt tttgtattct tggcgtattt tttgttcttc tgcataacgc
     3001 tctggatttt tcttataaaa atcttggtga tagtcttcgg ctttgtaaaa ttgtgacgct
     3061 ggtaatattt ttgttgcaat tgccttatca gcattaatcg tatttttaag ctgctcgata
     3121 taagtctcag cgagttcttt ttgatgatca ttagtgtaga aaatagctgt ttgatattga
     3181 ggaccacggt cttgatattg accacctgta tctaatgggt caatgactga gaaaaatatt
     3241 tctaataact tattgtatga gaataatgca acatcatatt gaatttcaac agtttctaaa
     3301 tgtccactcg tacctgattt tacttgttcg taagtagggt tttcaatatg tccgcccata
     3361 tatccagaag ttactttttc tatgccgtca aaggtgtcaa atggtttcgt catacaccaa
     3421 aagcaacctc cggcaaaata agctgtatta atattcattt ttgacatcct ttcattagac
     3481 cttagtacga tttattaaga aatcacttgc tttttgaatt gtttttatat aacgttaata
     3541 tgtgattatt atagtacaca aaaatctatt tttgaagata taaggtaggt aaatatggat
     3601 aaagaaacta atgacaacga atatagacgt caaagtgaac atcgcacttc ggcgcctaag
     3661 cgaaaaaaga agaagaaaat taggaaatta cctatcattc ttctgattgt tgtaatttta
     3721 cttatcgcat tagttgtata tattgtacat agttacaata gcggtgtaga atatgccaag
     3781 aaacatgcga aagatgttaa agtacatcaa tttaatggac cagtaaaaaa tgatggtaaa
     3841 atttctattc ttgtactcgg tgcagataaa gcacaaggtg gacaatcaag aacagattct
     3901 atcatggttg ttcaatatga ctttatcaat aaaaagatga aaatgatgtc tgtcatgcgt
     3961 gatatttatg cagatattcc agggtatgga aaacacaaaa ttaattcagc atacgcttta
     4021 ggtggtccag agctacttag aaaaacactt gataaaaatt taggaattaa tcctgaatat
     4081 tatgcagtag ttgattttac tggatttgag aaaatgattg atgaattaat gccagaaggt
     4141 gtaccaatta atgtcgaaaa agatatgtcg aaaaatattg gtgtatcttt gaaaaagggt
     4201 aaccataggt tgaatggtaa agaattactt ggttatgcaa gattccgtca cgaccctgaa
     4261 ggtgacttcg gacgcgtgcg acgtcagcaa caagtaatgc aaactttgaa aaaagaaatg
     4321 gttaatttta gaacagttgt taaattacca aaagttgcag gtattttaag aggctatgtg
     4381 aatacaaaca ttcctgattc agggatcttc caaacaggtt tgagttttgg tatccgaggt
     4441 gaaaaagatg ttaagtcatt gactgtgcca atcaagaact catacgaaga tgtcaataca
     4501 aatactgatg gtagtgcatt acagattaat aaaaacacaa ataaacaagc tattaaagac
     4561 tttttagatg aagattaaaa ataaa
//
LOCUS       DAKSQE010000149         4576 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.151, whole genome shotgun sequence.
ACCESSION   DAKSQE010000149 DAKSQE010000000
VERSION     DAKSQE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.151"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(188..544)
                     /locus_tag="O0V33_002450"
     CDS             complement(188..544)
                     /locus_tag="O0V33_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCX8977046.1"
                     /translation="MKKKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     ATIEKMISEKDESIEKNVKLPSDADVSVKKGDFVIVYKLADSDKIIKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
     gene            complement(822..1244)
                     /locus_tag="O0V33_002451"
     CDS             complement(822..1244)
                     /locus_tag="O0V33_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HCX8977047.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMIFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEIVTEQEVIDWLWETQD
                     KSFFSSKEDPELCVLRVVPQSIKLMNDKSLDTPIKIDL"
     gene            complement(1315..3420)
                     /gene="rsp"
                     /locus_tag="O0V33_002452"
     CDS             complement(1315..3420)
                     /gene="rsp"
                     /locus_tag="O0V33_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HCX8977048.1"
                     /translation="MTCQLKIHHTLSTIPSRNINNIMVLFSLTSKLNITINGETKDVS
                     NYIILINHGDIYNINHGENIIELMIPVFYFYQQDDDFFNGYLDRHLLQSSNYIKSLIA
                     DLISTPTSSSLMGKNIGQSIIDTLLKEAFIRIDHEYLPNIALSNPVFIDCVNYIHDNI
                     DAHLSLKDIAMHCNISESYCSNLFVRYLSMNFKDYFTSIKLVNAINLLLSTKHSITTV
                     SELAGFNSHTNFANQFKNYLHFSPKQFRSLVSKITEPPQIHFQQDNVSQFTELISTID
                     LTAQLATNTTDIHIDDFNPKDRSQRAKVFVRFSNFNELFQFIFNEYYDINFEHLPKPV
                     VFIDDIHDIEISQTNYNLLNRCFEKLFEKNIGLAIAIKSTQQFETMKQLILTFLQGNQ
                     DYKTSKKLVKFMLVFCSNSMTAEEIHLCHLKIKNKNKEIKYSVTVDGFLETYSTVEQV
                     YDVMQRLKFHYYFIDIENSKTATHLITKNQHYHQTDTHFEQYKKFILDSGISSTQFVY
                     NNLSVSGFKYTNDGKNPIQLSDIVYHLIALLRYGGGISYQLLDDHSNYISLYNKYGSP
                     LPLMHLYKMFRPFVNEDIEITNNYVLSRKDNNYHFLLFNKINDRYMSDVKQDFIFHNE
                     LPQDSLMIIKTLNHEHGSIQHLLPTSDQLVYIEKEILDELDKTNYPKTELAVQEETGR
                     TFELKLNHDEVKYICFKPS"
BASE COUNT         1651 a          670 c          645 g         1610 t
ORIGIN      
        1 tgcatgcgca aagcgcaggc gaactgaagc gaagtgtagg ggcttttatg catgcgctaa
       61 agcgcagggc atttgcaaat aaactggtat atttattctc aaaaaaacag cagtaagatg
      121 attttcaatt agaaatatct tactgctgct tttaatatct tatatattac tcaaaattaa
      181 agcttaatta gtgtttcatg tgcattttgc ccatgtcatg aattttcatc attaaaccat
      241 gtggtacatc gtcatgatca acttttttta ctttaataat tttatcagaa tcggctaatt
      301 tataaacaat tacaaagtcg ccttttttaa ctgaaacgtc tgcatctgaa ggtaatttaa
      361 cgttcttctc aatgctttca tctttttcac taatcatttt ttcaattgta gcattgtctt
      421 tcataattcc ataatatgta tctttctttc caccagtcat catcatgatt actagtacaa
      481 tacatacaat caacatgatg gcaattaaac taataccaag accaaaacct tttttctttt
      541 tcacggttca tcaatctcct taaatgtagt taattctgtt atacctaaaa gtacaacagt
      601 ttgcaaatgc agtatttata ttacataaaa ttaatttaaa aagacacacc ttaatcacaa
      661 taaatgtgaa taaaatgtgt ctaaaatatt gttatataat aaaaatatag ttaaaagtat
      721 taaacgcttt caaaatcgaa atatatataa ataggatact gttgaaattt atagctaaca
      781 tgttcaattg ctgactaatt ttaaaactaa aaacttacat attataaatc aattttaata
      841 ggtgtatcta atgatttatc attcattagc ttaatggatt gtggaactac tcttaaaaca
      901 cataactctg ggtcttcttt tgaactgaaa aagcttttgt cttgtgtttc ccatagccaa
      961 tcaatcactt cttgttctgt aacgatttcg attgtcgctt ccatttcaac gaaactacga
     1021 ttagttgtgt cgttatagcc taataaaaca tatgctgctg gattatcttt aatttctttg
     1081 accttagcag aatggatatt cgttttagta tataaagtaa gaccatcatt ataaaagatc
     1141 atatatctac tattaggttt gttattatat gcagttgata atacaccaac ttttgacgtt
     1201 gctagcacgt tttcaattgc ttgaattgct tgtgagttac tcatcctata tcatctcctt
     1261 atgatataaa tagcctttaa cgtaagaaac taaacacaag aggactgtta gtatttagct
     1321 tggtttaaag caaatatatt taacctcgtc gtgatttaac ttgagttcaa atgtacgacc
     1381 agtttcttct tgaactgcaa gctccgtttt agggtaattc gttttgtcta attcatctag
     1441 aatttctttt tctatataga caagttgatc gcttgttgga agcaaatgtt gaattgaacc
     1501 atgttcatga ttcaatgttt taataatcat caaagagtct tgaggtaatt cattatggaa
     1561 aatgaagtct tgttttacgt ctgacatata tcggtcatta attttattga ataataagaa
     1621 atggtagtta ttatctttac gactcaatac ataattattt gtaatttcaa tgtcttcatt
     1681 aacaaaaggt ctaaacattt tatataaatg cattaatggt agggggctac catatttgtt
     1741 gtacaaggaa atataatttg aatggtcatc taataattga tagctaatgc caccgccata
     1801 tcgcaataat gcgattaagt gatacactat gtcagataat tgaattggat tcttaccatc
     1861 attagtatat ttaaatccac ttacagacag gttattatat acaaattgtg ttgatgatat
     1921 acctgaatct aaaataaatt tcttatactg ttcaaaatga gtgtcagttt gatgatagtg
     1981 ttggttttta gtgataagat gtgttgcagt ttttgaattt tcaatatcaa taaagtaata
     2041 atggaattta agacgctgca tgacatcata aacttgttcc acggttgaat aagtttctaa
     2101 aaagccatcg actgtcacac tatatttaat ttctttattt ttgtttttaa ttttaagatg
     2161 acataaatga atttcttctg ctgtcataga gttagagcaa aatacgagca taaatttaac
     2221 taatttcttg cttgtcttat agtcttgatt gccttgtaaa aatgttagta ttaattgctt
     2281 catcgtttcg aattgttgtg ttgatttaat agcgattgct aaacctatat ttttttcgaa
     2341 aagcttttca aaacatctat ttaacaggtt gtaattcgtt tggctaatct caatatcgtg
     2401 aatatcgtca atgaaaacga caggttttgg taggtgctca aagttaatat cgtaatattc
     2461 attgaaaata aattgaaata gttcattgaa attactaaat cgaacaaata ctttggcacg
     2521 ctgacttcga tctttcggat taaaatcatc tatatgaata tctgttgtat ttgtagccag
     2581 ctgagctgtt aaatcaattg ttgaaattaa ctcagtgaat tgtgatacgt tatcttgttg
     2641 gaagtgtatt tgtggtggtt cagtaatctt ggaaacgagt gaacggaact gtttaggact
     2701 aaaatgcaag taattcttaa attgatttgc aaaatttgta tgactgttaa aaccggccaa
     2761 ttcagaaaca gttgtaatag aatgttttgt agaaagtaat aaattgatgg cgttaacaag
     2821 tttaatactt gtaaaataat ctttaaaatt catacttaaa tatcgaacaa aaagattaga
     2881 acaatatgat tcagagatgt tgcaatgcat cgctatatct tttaaagaca agtgcgcatc
     2941 aatattgtca tgaatatagt ttacacagtc tataaacacc ggattgctta aagcaatatt
     3001 tggtaaatat tcatgatcta ttcttataaa agcttcttta agtaaagtgt ctataatact
     3061 ttgacctata ttttttccca ttaaagaaga acttgttggt gtactgatta aatcggcaat
     3121 taatgactta atataattac ttgattgtaa taaatgacga tctaaataac cattaaagaa
     3181 atcatcatct tgttgataaa aataaaaaac gggtatcatt aattcgatta tattttcgcc
     3241 gtggttaata ttataaatat caccatgatt aattaagata atataattac ttacgtcttt
     3301 ggtttcacca ttaatcgtaa tattgagttt acttgtaagt gaaaatagca ccataatatt
     3361 attgatatta cgacttggta ttgtcgataa tgtatgatgt attttaagtt ggcatgtcat
     3421 atttctctct cctgcctaat atatattgaa atctatcatt taaatgtaaa acatggaaat
     3481 ttttaaaatc atggatagta tacctattat agttgaatat ttttgaaaag gtaatagtga
     3541 aaaataaaag aaattaaaga aaactcctag gaaaacgctt gtttttatcc atcgtgcaca
     3601 atataaaagt aaaattagaa agatagtaag caaaatttgg aaattttcga tagagtctaa
     3661 ttaaattatt tggtgcataa ttaatataga tttcaaacct atgtatttca aaaaacaata
     3721 catagccatt ttgaactaac ttgctaatct tttattgata ttcaactatg ttataaaaaa
     3781 agcaaattga ccaaaatgga agtttgaatc aaaaaacatt aaatgtaccc atgacttaaa
     3841 ttttatcgcc attatttaag tttggacata cacttttgtc atcaaacgct gaaaattagt
     3901 tcgattgaaa tcacttcttg ttataaaaat tcttagtagc cctgttacaa aatattcgct
     3961 acaaattatt caaatcaaca acaataacca tcaactaaga aattttaaac aatctactaa
     4021 aaaataaagc gtttaaaagt tctgtttaga aaacatcaag ataagtataa acatctagaa
     4081 aagtgaacat ctaagaaaat ccatctagtt attgccgtaa caattagaat aatcatttga
     4141 aaaagtgcat tagacaacga aattcaccat caagataatg aagcaacaag aaacagcaca
     4201 catcaatgaa ttaacacctt agattcatca tgagaaaatg catggcacaa ctagatttac
     4261 catcaagata attcttaagc agcgaagaat taacatctag aaatgcataa acatcttaga
     4321 ttttacatca agagatgcga taactataaa caactagaaa cgtcaccaag atagtgcaac
     4381 aacaaagaat cagcatcttg aaatcgcact aacatctaag aatcaacatc aagtattata
     4441 ttaacatcat agataacttc atcaagacag gcatccacat ctaagaaact catctagaat
     4501 agctaacacc taacatcaaa gaatattcat caagaacagt atctaacaga actttttgac
     4561 tatatatgaa ttaatt
//
LOCUS       DAKSQE010000150         4545 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.152, whole genome shotgun sequence.
ACCESSION   DAKSQE010000150 DAKSQE010000000
VERSION     DAKSQE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.152"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(182..475)
                     /locus_tag="O0V33_002453"
     CDS             complement(182..475)
                     /locus_tag="O0V33_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HCX8977049.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGK
                     HYVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
     gene            complement(855..1250)
                     /locus_tag="O0V33_002454"
     CDS             complement(855..1250)
                     /locus_tag="O0V33_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent arsenate reductase"
                     /protein_id="HCX8977050.1"
                     /translation="MDKKTIYFICTGNSCRSQMAEGWGKEILGEGWNVYSAGIETHGV
                     NPKAIEAMKEVDIDISNHTSDLIDNDILKQSDLVVTLCSDADNNCPILPPNVKKEHWG
                     FDDPAGKEWSEFQRVRDEIKLAIEKFKLR"
     gene            complement(1268..2560)
                     /gene="arsB"
                     /locus_tag="O0V33_002455"
     CDS             complement(1268..2560)
                     /gene="arsB"
                     /locus_tag="O0V33_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HCX8977051.1"
                     /translation="MMTILAIVIFLLTLTFVIWQPKGLDIGITALIGAVVAIITGVVS
                     LSDVLEVTGIVWNATLTFVAVILISLILDEIGFFEWSAIHMVKASNGNGLKMFVFIML
                     LGAIVAAFFANDGAALILTPIVLAMVRNLGFNQKVISPFIIASGFIADTTSLPLIVSN
                     LVNIVSADYFDIGFIEYFSRMIIPNIFSLIASILVLWLYFRKSIPKTFDTENLSDPKS
                     AIKDSKLFKLSWIVLAVLLVGYLVSEFIQIPVSIIAGIIAFIFVILARKSKAVHTKQV
                     IKGAPWNIVVFSIGMYLVVFGLKNVGITTVLGDVLTNISNYGLFSSIMGIGFIAAFLS
                     SIMNNMPTVLIDAIAIGQSSATGILKEGMVYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKGVKISWGTYFKTGIIITIPVLFVTLLGLYLTLIIF"
     gene            complement(2557..2871)
                     /gene="arsR"
                     /locus_tag="O0V33_002456"
     CDS             complement(2557..2871)
                     /gene="arsR"
                     /locus_tag="O0V33_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCX8977052.1"
                     /translation="MSYKELSTILKILSDSSRLEILDLLSCGELCACDLLEHFQFSQP
                     TLSHHMKSLVDNELVTTRKDGNKHWYQLNHAILDDIIQNLNIINTSNQRCVCKNVESG
                     DC"
     gene            complement(2932..3267)
                     /locus_tag="O0V33_002457"
     CDS             complement(2932..3267)
                     /locus_tag="O0V33_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCX8977053.1"
                     /translation="MITSLPMMNEVIGNPLLDKFMKDLIIQILAMISEQERNESKRRQ
                     AQGIQVAKEKGIYKGRPILYSPNAKDPQKRLVYYRVVELLEQGKSISTIAKEVGITRQ
                     TIYRIKNSK"
     gene            3587..3799
                     /locus_tag="O0V33_002458"
     CDS             3587..3799
                     /locus_tag="O0V33_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977054.1"
                     /translation="MNKKNELDIAFIMSTVLNVLFLVGLTFLVQLENLYILIPYTILG
                     FINAVYLTVRAINITKNRRFCCKVKK"
     gene            complement(3834..4508)
                     /locus_tag="O0V33_002459"
     CDS             complement(3834..4508)
                     /locus_tag="O0V33_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISSau6 family transposase"
                     /protein_id="HCX8977055.1"
                     /translation="MNYFRYKQFDKDVITVAVGYYLRYALSYRDISEILRERGIYVHH
                     STIYRWVQEYAPILYQIWKKKNKQAYYKWHIDETYIKIKGQWNYLYRAIDADGHTLDI
                     SLRKKRDNHSAYTFIKRLIKQFGKPQMIITDQAPSTKVAMSKVIKDFKLTPNCHCTSK
                     YLNNLIEQDHRHIKVRKISYQSINTAKNTIKGIECIYGLYKKNRRSLQIYGFSPCRVI
                     SIMLAS"
BASE COUNT         1560 a          766 c          644 g         1575 t
ORIGIN      
        1 gaactttcag gtataatttc ttcattttct ttgaagaaaa ataaaaataa tagccctgaa
       61 attaataata aagtactaaa atctaaaaat aatttgaatt ttttcatatt gcctctttca
      121 agagaataga cagaattaag tttacttaat tctgtctatt ctccttaatt aatatatgta
      181 attagtgaag actataaaat gttttgtttc caccccaagc ctttggagca gatgccttag
      241 attgaacacc aggtcgagca acaccactaa aagatgaata tttccctact gctgttgaac
      301 catgatttct tttattgtga ctataataag accatacgta atgttttcca ataccatggc
      361 tccaaacacc accagctaca tgtactgttg cagcttcagc tttataagtt acacctaata
      421 aagtgccgaa taagattgta ctagcaatac aactagaaac aaatttcttt ttcataaaaa
      481 cacctccatt taatattctt tatttattat agcaaaaata aataaaatag agaatgagta
      541 aaaataatct aaaagtgttt ttttgctctg ttgcttgttt tgttcatcta tatataatat
      601 taattctatt atccttaaat taactatcat taaacaacga tatgacaatg tataataaat
      661 acaatttcac tacctataat tcaacattat gtaaactaaa gtatttcaaa taattaactg
      721 tattaatact ctcatcatga ttgcgttata aacgttcgtt aatgagaaaa agagagggca
      781 tattgtatat ggcacaaatt ttgttcttta aatggcaaaa cacctcttat taactaaata
      841 agaggtgttt atgattatct caatttaaac ttttctatag ctaatttaat ctcgtctcta
      901 acacgttgga attctgacca ttctttacct gctggatcat caaaacccca atgctctttt
      961 ttaacgtttg gtggtaaaat aggacaatta ttgtctgcat cactacataa cgttacgacc
     1021 aaatctgatt gttttaaaat atcattatca atcaagtctg acgtatggtt tgatatatca
     1081 atatctactt ctttcatagc ttctattgct ttaggattaa caccatgtgt ttcaatacca
     1141 gcagaataga cattccaacc ttcacccaat atttcctttc cccaaccttc agccatttgg
     1201 ctacgacaag agtttcctgt acatataaaa taaattgttt tcttatccat aattaaagcc
     1261 ccttttctta aaatatgatt agtgtaaggt ataaacctaa gagtgttaca aataggactg
     1321 gaatagtgat gataattcca gttttaaagt atgttcccca cgaaatcttc acaccttttt
     1381 gtgttaagac atgtagccac aataatgttg ctaaagaacc aattggcgta attttaggtc
     1441 ctaaatcaga acctatgaca ttcgcataaa ccatgccttc ttttaatatt cctgtagcac
     1501 tggattggcc aatcgctatt gcatctatta aaacagttgg catgttgttc ataatcgaag
     1561 ataagaaagc agctataaag ccgataccca tgatgctgct aaataaccca taatttgaaa
     1621 tgtttgttaa aacatcccca aggaccgttg taatacctac attttttagt ccaaacacaa
     1681 caagatacat accaatagag aatacaacga tattccatgg tgcaccttta atcacttgtt
     1741 ttgtatgaac tgctttagat ttacgagcta atattacaaa gataaaagca ataataccag
     1801 caataattga tacaggaatt tggataaact cgctaacaag atatccaacg agtagtactg
     1861 ctaatactat ccatgaaagc ttaaataact tagaatcttt aattgcactt ttaggatctg
     1921 atagattttc tgtatcgaac gttttaggta tggattttct gaaatataac cataaaacga
     1981 gaatactagc aatcagagag aatatattag gaatgatcat tcggctaaaa tattcaataa
     2041 atccaatatc gaagtaatct gcagaaacga tattaactaa gttacttaca attaagggaa
     2101 gtgatgtagt atcagcaata aaaccactgg caataataaa gggggaaatc actttttgat
     2161 taaatcctag attccttacc atcgctaata caataggcgt taagattaaa gctgcaccat
     2221 catttgcgaa aaatgctgct acaattgctc caagtaacat aataaaaaca aacattttta
     2281 agccattacc gtttgaagcc ttgaccatat gtatcgcaga ccattcaaaa aaaccaattt
     2341 catctaatat taatgaaata agaataacag ccacaaaagt taaagttgca ttccaaacaa
     2401 tacctgttac ttctaataca tcagaaagac ttacgactcc tgtaatgata gcaacaacag
     2461 ctccaattaa agctgtaata ccaatatcta aaccttttgg ttgccaaatc acaaaggtta
     2521 aagttaaaag aaaaattaca attgctaaaa tagtcatcaa cagtcacctg attccacatt
     2581 tttacataca catctttgat tagatgtatt aatgatgttc aagttttgga taatatcatc
     2641 taaaatagca tgattaagtt gataccaatg tttattgccg tcttttcgtg ttgtaactaa
     2701 ttcattatct actaatgact tcatatgatg acttagtgta ggttgtgaga attgaaagtg
     2761 ttctaataag tcacaagcgc atagctcacc acaagaaagt aaatctaata tttctaacct
     2821 acttgaatct gataaaattt ttaatattgt tgatagttct ttataagaca tgaataaacc
     2881 tcctagacat taaatagatt aacatctata tagattattg tctatgttat tttatttact
     2941 attttttatt ctatatatag tttgacgtgt aataccaact tctttagcta tagtacttat
     3001 agatttaccc tgttcaagta attcaacaac tcggtaataa actaaacgct tttgtgggtc
     3061 tttagcattg ggagaataca gaataggtct ccctttatat ataccttttt ctttggcgac
     3121 ttgaatccct tgagcttgtc gacgtttact ttcatttctt tcttgttctg aaatcattgc
     3181 taaaatttga ataattaaat ctttcataaa tttatctagt agtggatttc caatgacttc
     3241 attcatcata ggtaaactcg ttatcatgag ctgaacttct tttcgaagat ttttcgtaga
     3301 tataatttgc taataaactt cctaaaactg aataaagaaa aatagttaaa ctcatgtaat
     3361 cactccataa ttctgaagtg tctccatgtc tacgcataga cacgcattta tgccgagaaa
     3421 atttattgtg cgttgagaag aacccttaac taaacttgca gacgaatgtc ggcatagcgt
     3481 gagctattaa gccgaccatt cgacaagttt tgggattgtt aagggttccg aggctcaaca
     3541 tcaataaagc aactatggta aaattttaaa aaaggaggaa tgtattttga ataaaaaaaa
     3601 tgagttagat atagctttta ttatgtctac cgttttgaat gttttatttt tagttgggtt
     3661 aactttctta gttcaattag aaaatttata tattttaatt ccatatacta ttttgggttt
     3721 tataaatgca gtttatctaa ctgttagggc tataaatatt actaaaaata ggaggttctg
     3781 ttgcaaagtt aaaaaataag tgtctaacct tatgtttaaa ataatacttc gtattaacta
     3841 gctagcatga tgctaattac acggcatggc gaaaacccgt agatctgaag agatctgcgg
     3901 ttctttttat atagtccata aatacattca atgcctttga tcgtattctt tgccgtattg
     3961 atactttgat aacttatttt ccttactttg atatgacgat gatcttgttc aatgagatta
     4021 tttaaatatt tagaggtaca atgacagttg ggggtaagtt taaaatcttt aatcactttg
     4081 gacattgcca ccttcgttga aggggcttgg tctgtaatta tcatttgagg tttgccaaat
     4141 tgtttaataa gacgtttaat aaacgtatat gctgagtgat tatctcgttt cttacgtaac
     4201 gaaatatcta gtgtatggcc atctgcatca atagcgcgat ataaataatt ccattgccct
     4261 ttaattttga tatacgtctc atctatatgc catttataat aagcttgctt attctttttc
     4321 ttccaaattt gatacaaaat aggagcatat tcctgtaccc atcgataaat tgttgaatga
     4381 tgaacataga tgcctcgttc tctcaatatt tcagatatat cacgataact taatgcatat
     4441 cttagatagt agccaacggc tacagtaatg acatctttgt caaattgctt atatctgaaa
     4501 taattcatcc tctgcacctc atttttgttg actattatac tatat
//
LOCUS       DAKSQE010000151         4528 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.153, whole genome shotgun sequence.
ACCESSION   DAKSQE010000151 DAKSQE010000000
VERSION     DAKSQE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.153"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            137..1312
                     /gene="rpsA"
                     /locus_tag="O0V33_002460"
     CDS             137..1312
                     /gene="rpsA"
                     /locus_tag="O0V33_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCX8977056.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDDIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLESEEEDNPTIGDMIGDKLKNLKL"
     gene            1534..2844
                     /gene="der"
                     /locus_tag="O0V33_002461"
     CDS             1534..2844
                     /gene="der"
                     /locus_tag="O0V33_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCX8977057.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEADVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTDAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            2861..3859
                     /locus_tag="O0V33_002462"
     CDS             2861..3859
                     /locus_tag="O0V33_002462"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCX8977058.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQEAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQYADIYLMALPTKAMREVATQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKN
                     VSKLTQDLFMNDYLRVYTNDDLVGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            4030..4302
                     /locus_tag="O0V33_002463"
     CDS             4030..4302
                     /locus_tag="O0V33_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCX8977059.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
BASE COUNT         1630 a          606 c          906 g         1386 t
ORIGIN      
        1 taatacacag aatgagatta agtgacatat ttatggatac gactaattta ctaaatgtaa
       61 taaaggtaga atttcttgac aattctgtca gtttataaga tgttataaat atgtagtgta
      121 taaggaggcg aacaagatga ctgaagaatt caatgaatca atgattaacg atattaaaga
      181 aggtgacaaa gtcactggcg aggtacaaca agttgaagac aagcaagttg ttgttcatat
      241 caacggtggt aaatttaatg ggattattcc tattagtcaa ctatctacgc atcatattga
      301 tagcccaagt gaagttgtaa aagagggcga cgaagttgaa gcatatgtca ctaaagttga
      361 gtttgatgaa gaaaatgaaa ctggagctta catcttatct agaagacaac ttgaaactga
      421 gaagtcttat agttatttac aagaaaaatt agataataat gaaatcatcg aagcgaaagt
      481 aacagaagta gttaaaggtg gtttggttgt tgatgtagga caaagaggtt ttgttccggc
      541 ttcactaatt tcaacagact tcattgagga tttctctgtg tttgatggac aaacaattcg
      601 tattaaagtt gaagaattgg atcctgaaaa taatagagtc attttaagcc gtaaagcagt
      661 tgaacaagaa gaaaacgatg ctaaaaaaga tcaattatta caatctttaa atgaaggcga
      721 tgttattgat ggtaaagtag cgcgtttaac tcaatttggt gcatttatag acattggcgg
      781 tgtcgatggt ttagtgcatg tatctgaact ttctcacgaa catgttcaaa caccagaaga
      841 agtagtttca attggtcaag atgttaaagt taaaattaaa tctattgata gagatacaga
      901 acgtatttca ttatcaatca aagatacgtt accaacacct ttcgaaaata ttaaaggtca
      961 attccacgaa aatgatgaca ttgaaggtgt cgtagtaaga ttggcaaact ttggtgcatt
     1021 tgttgaaatt gcaccaggtg tacaagggct tgtacatatt tctgaaattg cacacaaaca
     1081 cattggtacg ccaggtgaag tattagaacc tggtcaacaa gtaaatgtta aaatattagg
     1141 tattgatgaa gagaatgaaa gagtatcact atctattaaa gcaacattac caaacgaaga
     1201 tgttgttgaa agtgatcctt ctacgactaa ggcgtactta gaaagcgaag aagaagataa
     1261 tccaacaatt ggcgatatga ttggtgataa acttaaaaat cttaaactat aatttaatat
     1321 ttaatagtca actccacatg tttatgattg catgtggagt atttttatgt aacaaaatat
     1381 atccggaatg ataacgtgga caaatttaac taagtgtttt ttaagataaa gttttaagtg
     1441 ctgattttta tcattacagt aataaactca ttttgaatac acagtctcat gtgatattat
     1501 taaaaagata taagaaagag aggaagttag cttatgacta aacctatagt agctattgta
     1561 ggtaggccta atgtaggtaa atctacaatt tttaatagaa tagttggaga acgtgtttcg
     1621 attgttgaag atacgccagg tgtaacacga gatcgtattt attcttcagg tgagtggtta
     1681 acacatgatt tcaatattat tgatacaggt ggtattgaaa ttggtgatgc accattccaa
     1741 acacaaatta gagcgcaggc agaaatcgcc atagatgaag cggatgttat tatttttatg
     1801 gttaacgtgc gtgaaggatt gacacaaagc gatgaaatgg tcgctcaaat tttatacaaa
     1861 tctaaaaaac cggtcgtatt agcggttaac aaagtagata atatggaaat gcgtacagac
     1921 gtgtatgatt tctattcatt aggatttggt gaaccgtatc cgatatcagg gtcacatggt
     1981 ttaggtcttg gtgacttgtt agatgcagtt gtttctcatt ttggtgaaga ggaagaagat
     2041 ccttatgatg aagatacaat tcgactatcc attattggac gaccaaacgt aggtaaatca
     2101 agtttagtaa atgctatttt aggtgaagat cgcgttatcg tttctaatgt tgcagggaca
     2161 acgagagacg ctattgatac agagtatagt tatgatggac aagattatgt tttaatcgat
     2221 actgctggta tgcgtaaaaa aggaaaagta tatgaatcaa ctgagaaata ttcagtatta
     2281 agagctttaa aagcgattga acgttcaaat gttgttttag tggtcattga tgcagaacaa
     2341 ggcattattg aacaagataa acgtgttgca ggatatgcac atgaacaagg taaagcagtc
     2401 gtgattgtcg taaataaatg ggatactgtg gaaaaagata gtaaaacgat gaagaaattt
     2461 gaagatgaag tacgtaaaga attccaattt ttagattatg cacaaattgc ttttgtgtct
     2521 gctaaagaac gcacaagatt acgtacatta ttcccttaca ttaatgaagc aagtgaaaac
     2581 cataaaaaac gtgttcaaag ttcaacttta aatgaagtcg ttactgatgc aatttccatg
     2641 aaccctacac caacagacaa aggtagacgt ttgaatgtct tttatgcaac acaagttgct
     2701 atagaaccac cgacatttgt tgtatttgtt aatgatgtag aattaatgca tttttcttat
     2761 aaacgctatt tagagaatca aatccgtgcc gcttttggtt ttgaaggtac accaattcat
     2821 attatagctc gaaagagaaa ttaacgattg ggggataaca atgactaaaa ttaccgtttt
     2881 tggtatggga agttttggga cagcccttgc caatgttctt gcagaaaatg gacatgatgt
     2941 tttgatgtgg ggtaaaaatc aagaagctgt tgatgaatta aatacatgtc atacaaataa
     3001 aaagtattta aaatacgcga aattagatgt taacatcatc gctacttcag atatgaccaa
     3061 agcaattcaa tatgcagata tttacttaat ggctttacct actaaagcaa tgcgagaagt
     3121 tgctactcaa attaatgata agctgacatc taaaaagact tttatacatg ttgctaaagg
     3181 tattgaaaat gggacattta aacgtgtgtc agaaatgatt gaagattcta tttcacctga
     3241 atataatgca ggtattggcg tgttgtcagg gccaagtcat gcggaagaag ttgtagtcaa
     3301 gcaaccaact acagttgctg catcatcaaa agataaaaat gtaagtaaat taacgcaaga
     3361 tttatttatg aatgattatt tgcgtgtgta cacgaatgat gacttggttg gtgttgaact
     3421 tggtggtgca ttgaaaaata tcatcgcagt agcaagtggt atcgtagctg gaattggcta
     3481 cggtgataat gcaaaagctg cattaatgac tcgtggctta gcggaaatta gcagattagg
     3541 tgaaaagtta ggtgccgatc ctatgacatt tctaggttta ggtggtatcg gtgacttaat
     3601 cgttacttgc acatcaacac attctcgaaa tttcacatta ggatataagc ttggacaagg
     3661 tgaatcgatg gatcaagcat tatctgaaat gaatatggtt gttgaaggta tttatacaac
     3721 taaatccgtt tatcatttag ctaaagaaaa aaatgtggat atgccaatta caaatgcatt
     3781 atatagagta ttatttgaaa atatctcagt aaaagaatgc gtaaaagatt taatggagcg
     3841 cgataaaaaa tctgaataaa atgtgtatta ttacacatat acagctaata attacgtaaa
     3901 atgaatagaa atggcttaaa ttcaacgttt ttgccacata aatgattgcg atttatgtaa
     3961 tgcttgtgtt aaagttttac cataatgata taaacatatc attatcaaac ctcaggaggt
     4021 gaatgtctaa tgaacaaaac agatttaatc aatgcagttg cagagcaagc tgatttaact
     4081 aaaaaagaag ctggttcagc agtagatgct gtattcgaat caatccaaaa ctcacttgct
     4141 aaaggtgaaa aagtacaatt aattggtttc ggtaactttg aggtacgtga acgtgctgca
     4201 cgtaaaggtc gtaaccctca aactggtaaa gaaattgata tcccagcaag taaagttcca
     4261 gcattcaaag ctggtaaagc attaaaagat gctgtaaaat aattttactt aaaaagccct
     4321 gaataagggc tttttatttt gcttttaata cttacaacct gtacataaat tgtaatgttc
     4381 ttctaagttt ttaatctttg gaacattcat tgtttaagcg ttcaaaatat tacatgacat
     4441 aatagttttg ataaaaatag caactacaaa atatacatat gaataatgag attaattgtt
     4501 agcgttgagt ttacttaaaa ggtaacca
//
LOCUS       DAKSQE010000152         4504 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.154, whole genome shotgun sequence.
ACCESSION   DAKSQE010000152 DAKSQE010000000
VERSION     DAKSQE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.154"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            182..1138
                     /locus_tag="O0V33_002464"
     CDS             182..1138
                     /locus_tag="O0V33_002464"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCX8977060.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            1338..1817
                     /locus_tag="O0V33_002465"
     CDS             1338..1817
                     /locus_tag="O0V33_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCX8977061.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1832..2671
                     /gene="fakB2"
                     /locus_tag="O0V33_002466"
     CDS             1832..2671
                     /gene="fakB2"
                     /locus_tag="O0V33_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCX8977062.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTFNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSVGVAHANVIEYVDKLKKVFNEAFHVDNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2757..3290
                     /gene="msrA"
                     /locus_tag="O0V33_002467"
     CDS             2757..3290
                     /gene="msrA"
                     /locus_tag="O0V33_002467"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCX8977063.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHADNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DKHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            3283..3711
                     /gene="msrB"
                     /locus_tag="O0V33_002468"
     CDS             3283..3711
                     /gene="msrB"
                     /locus_tag="O0V33_002468"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCX8977064.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMVRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            3723..4223
                     /locus_tag="O0V33_002469"
     CDS             3723..4223
                     /locus_tag="O0V33_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCX8977065.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEYVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            4223..4444
                     /locus_tag="O0V33_002470"
     CDS             4223..4444
                     /locus_tag="O0V33_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCX8977066.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
BASE COUNT         1637 a          649 c          821 g         1397 t
ORIGIN      
        1 gaaacataga tcacgttatg atgtggcagg tacttaatca tcattttata aataaagtaa
       61 cggaattcat gatattaata tcatattcct aaaatgagtg ataacaaaat gctacataaa
      121 gttaaattat atcgaactaa atatacatac tataaataat aaaaatgagg tgttatcgca
      181 tatgttgaat tcatttgatg cagcatatca cagtctttgt gaagaagttt tagaaatagg
      241 aaatacacga aatgatcgca caaatacagg tacgatttcg aaatttggtc atcaacttcg
      301 ctttgactta tctaaaggat ttccactatt aacgacaaag aaagtttctt ttaaattagt
      361 agcgaccgaa ttattatggt tcattaaagg agatacaaat atccaatact tattaaagta
      421 taataataat atttggaacg aatgggcttt tgaaaattat atcaaatcag acgagtataa
      481 aggtccagat atgacagatt tcgggcatcg tgcattgagt gatcctgaat ttaacgaaca
      541 atataaagaa caaatgaaac aatttaagca acgtattctt gaagatgata catttgcgaa
      601 gcaattcggt gatttaggaa atgtttatgg taaacagtgg cgagattggg ttgataaaga
      661 tggtaatcat tttgatcaac ttaaaacagt aattgagcaa attaagcata atccagattc
      721 aagacgacac atcgtatctg catggaatcc aacagaaatt gatacaatgg cacttccgcc
      781 ttgtcataca atgttccagt tttatgttca agatggtaag ttaagttgcc agttatacca
      841 acgtagcgca gatatctttt taggtgtgcc atttaatatc gcaagctacg ctttattgac
      901 gcaccttatt gccaaagaat gtggacttga agtaggagaa tttgtgcata catttggaga
      961 tgcacatatt tattcaaatc atattgatgc gattcaaaca caattagcac gtgaaagctt
     1021 caatcctcca acattaaaaa ttaacagtga taagtctatt tttgacataa attatgaaga
     1081 tttggaaatt gttgactatg aatcacatcc agcaataaaa gctccaatag cagtgtagtc
     1141 gttgcatagt tagctaacca tatagacatc aaaatgacat caaagtattt tcaagtgcaa
     1201 aaaagtactt ttttgtgtta aacgttttca taaattatgc aaaatcatta tttctatcac
     1261 actttatgat aaaaattgtg ttaaattaaa gataacttag taattaaaaa tgaaatgata
     1321 gaagaaggag gataatcatg actttatcca ttctagttgc acatgacttg caacgagtaa
     1381 ttggttttga aaatcaatta ccttggcacc taccaaatga tttgaagcat gttaaaaaat
     1441 tatcaacagg tcatacttta gtaatgggtc gtaagacatt tgaatcgatt ggtaaaccac
     1501 taccgaatcg tcgaaatgtt gtacttactt cagatacaag tttcaacgta gagggcgttg
     1561 atgtaattca ttctattgaa gatatttacc aactaccggg ccatgttttt atatttggag
     1621 ggcaaacatt atttgaagaa atgattgata aagtggacga catgtatatt actgttattg
     1681 aaggtaaatt tcgtggtgat acgttctttc caccttatac atttgaagac tgggaagttg
     1741 cctcttcagt tgaaggtaaa ctagatgaga aaaatacaat tccacatacc tttctacatt
     1801 taattcgtaa aaaataaggg ggaaaacgac catgacaaaa cagattatag taacagactc
     1861 aacatccgat ttatctaaag aatatttaga agcaaacaac atacatgtaa ttcctttaag
     1921 tttaactatt gaaggagctt catacgttga ccaagtagat attacatcag aagaatttat
     1981 taatcatatt gaaaatgatg aagatgtaaa gacaagtcag ccagccatag gtgaatttat
     2041 atctgcttat gaagaactag gaaaagatgg ttctgaaatc ataagtattc atctttcttc
     2101 aggactaagt ggtacattta acactgctta ccaagcaagt caaatggtag atgctaatgt
     2161 aactgttatt gattcaaaat ctatttcttt tggtttaggg tatcaaatac aacacctagt
     2221 agagcttgta aaagaaggtg tctcaacttc tgaaatagtt aaaaagttaa atcatttaag
     2281 agaaaacatt aaattatttg tagttatagg gcaattgaat caattaatta aaggtggcag
     2341 aattagtaaa acaaaaggtt tgattggtaa tcttatgaaa attaaaccaa ttggtacact
     2401 agatgatggt cgcttagagc ttgtgcacaa tgcgagaact caaaattcta gtatccaata
     2461 cttgaaaaag gaaattgctg aatttatagg agatcatgaa atcaaatccg ttggtgtcgc
     2521 acatgctaac gtcattgaat atgttgataa attgaagaaa gtttttaatg aagcttttca
     2581 tgtggataat tacgatataa atgtaactac accagttatt tctgcacata ctggtcaagg
     2641 tgcgattggc ctcgtagtcc ttaagaagta aatttaatct tttcagtgtt aattacttcc
     2701 atttcaatcc tttatagact aaatttataa ttagatagat agaggaggta attcatatga
     2761 caaaagaata tgcaacatta gcaggaggat gtttctggtg catggttaaa ccatttacat
     2821 catatccagg catcaagtca gtcgtatctg gttatagtgg cggtcatgct gataacccaa
     2881 cttatgaaca ggtatgtacg aatcaaaccg gccatgtcga agcagtacaa attacgtttg
     2941 atccagaggt tacttccttt gaaaatatat tagacatata tttcaaaaca tttgacccaa
     3001 ccgatgatca agggcaattt ttcgatagag gcgaaagcta tcaaccagtc attttctatc
     3061 atgataagca tcagaaaaag gctgctgagt ttaaaaagca acaattaaat gaacaaggta
     3121 ttttcaagaa accagtgatt acacctatta aaccatataa aaatttctat ccagctgaag
     3181 actaccatca agattattat aaaaagaacc cggtacatta ttaccaatat caacgtggtt
     3241 caggtagaaa agcgtttata gaatcacatt gggggaatca aaatgcttaa aaaagataaa
     3301 agtgaactaa cagatataga atatattgtt acacaagaaa atggcactga accaccattt
     3361 atgaatgaat attggaatca ttttgctaaa gggatttatg tagataagat ttctggtaaa
     3421 cctttattta catctgaaga aaagtttcat tctgagtgcg gatggcctag cttttccaaa
     3481 gcgcttgatg acgatgaaat tatagaatta gtcgacaaat catttggtat ggtaagaact
     3541 gaagtgcgtt cagaagaatc aaatagtcat ttaggacacg tctttaatga tggacctaaa
     3601 gaaagtggcg gcttaagata ctgtatcaat tccgctgcaa ttcaatttat tccatatgaa
     3661 aaattagaag aattgggtta tggcgattta atatcacatt ttgataagta ggagtggaaa
     3721 gtatgtttaa aaaattattc ggaaaaggca aagaagttca gaaagatatc gcaatttatg
     3781 caccattaac tggagaatac gtgaaaattg aagatattcc agatcctgta ttcgcacaaa
     3841 aaatgatggg cgaagggttt ggtattaatc caactgaagg agaagttgtg tctccaattg
     3901 caggacgtgt tgacaatgtc tttccaacta agcatgcaat tggtttaaaa gcagataacg
     3961 gcttagaatt attagttcat atcggtttag acacagttca attagacggt gaaggatttg
     4021 aagtgttagt gtctagtggt gatgaagtta acgtcggaga tccattagta agattcaacc
     4081 ttgaatatat taataataat gctaaatctg ttatttcacc aattataatt actaacactg
     4141 atcaagcggc ttctattaat atttatgatg aaaatgctgt gattaaaggt gaaactaaag
     4201 tgattgatgt gacaatgaac taatgaaaaa ctattcgttt tatcaatttg tcatgacagt
     4261 gcgtggtcga cacgacgata aaggtcgtct agcagaagag atatttgacg atcttgcttt
     4321 cccaaaacac gatgatgatt ttaacatact gtctgattat attgagacac atggtgattt
     4381 cacattgcca atgtctgtat ttgatgatct atatgaagaa tatacggaat ggctaaaatt
     4441 ttaatataat ttttaataat aatagttaga accagggtga tgcaagttgt tatcctggtt
     4501 ttaa
//
LOCUS       DAKSQE010000153         4339 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.155, whole genome shotgun sequence.
ACCESSION   DAKSQE010000153 DAKSQE010000000
VERSION     DAKSQE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.155"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..193
                     /locus_tag="O0V33_002471"
     CDS             <1..193
                     /locus_tag="O0V33_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977067.1"
                     /translation="ENDFPHPFKKLIRWSNGIAAGADIQIFVTNYINENDLHIQNSLI
                     VAIVCLLSFLSTYCSHSKK"
     gene            complement(208..348)
                     /locus_tag="O0V33_002472"
     CDS             complement(208..348)
                     /locus_tag="O0V33_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977068.1"
                     /translation="MLQKFRIAKEKNKLKLKLLKHASYCLERSNNPELLRAVAELLKK
                     VN"
     gene            complement(379..576)
                     /locus_tag="O0V33_002473"
     CDS             complement(379..576)
                     /locus_tag="O0V33_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977069.1"
                     /translation="MQAQNKKVIYYYYDEAGNRRPVNIQYNDGYDLMIDPRFIEMTLE
                     RHPHLKNNFYGLIDGKEFKLD"
     gene            complement(592..1344)
                     /locus_tag="O0V33_002474"
     CDS             complement(592..1344)
                     /locus_tag="O0V33_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCX8977070.1"
                     /translation="MQALQTKSNIGEMFNIQEKENGEIAISGRELHQALEVKTPYKKW
                     FERMSDYGFEENIDYIVTDIFVHNPLGGRQNQTDHALTLDTAKEIAMIQRSEPGKRAR
                     QYFIQVEKAWNSPEMIMQRALKIANNTINQLETKIERDKPKIVFADAVATTKTSILVG
                     ELAKIIKQNGINIGQRRLFEWLRQNGFLIKRKGVDYNMPTQYSMERELFEIKETSITH
                     SDGHTSISKTPKVTGKGQQYFVNKFLGEKQTS"
     gene            1395..1724
                     /locus_tag="O0V33_002475"
     CDS             1395..1724
                     /locus_tag="O0V33_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977071.1"
                     /translation="MTDEAKFVLLQLYSIYLDRIDEGMSKRSASYFGSDESSFNAFFL
                     GFNFEDYIDAVLELKHRDFVIASAEDGGFLEMALSREGIAYSESESKKDYKTLMGLIR
                     DLKKLII"
     gene            complement(1713..1928)
                     /locus_tag="O0V33_002476"
     CDS             complement(1713..1928)
                     /locus_tag="O0V33_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCX8977072.1"
                     /translation="MNIQVATKLAMEKGISIRRENQDVYGILPTNLQRYQCLVVSRHY
                     KKKRQTAAGRWQPSADDLIADDWILDY"
     gene            complement(1944..2207)
                     /locus_tag="O0V33_002477"
     CDS             complement(1944..2207)
                     /locus_tag="O0V33_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCX8977073.1"
                     /translation="MKTLKELRTDYGLTQKELGDLFKVSSRTIQNMEKDSTNIKDSLL
                     SKYMSAFNVKYDDIFLGNEYENFVFTNDKKKSIILAFKEKQTS"
     gene            complement(2204..2377)
                     /locus_tag="O0V33_002478"
     CDS             complement(2204..2377)
                     /locus_tag="O0V33_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCX8977074.1"
                     /translation="MFLAIIIFLSLFILLTSFFLIIRNFHIIVKFFTKKTYLMLTIRK
                     FRIILGLRKAVTT"
     gene            2340..3053
                     /locus_tag="O0V33_002479"
     CDS             2340..3053
                     /locus_tag="O0V33_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCX8977075.1"
                     /translation="MNKERNIIIAKNIRKFLNDSNMSQKKLAELINIKPSTLSDYLNL
                     RSNPSHGVIQRIADVFEVGKSDIDTTYKDDNDITSIYNKLTPPRQENVLNYANEQLEE
                     QNSKGDNVVDINSYKQEKTPVNVNGCVSAGVGERLHDETLFTEMVKGPIPTHDLALKV
                     NGDSMEPMFKDGEIIFVEKTHNIKNGQIGIFIIEEEAYVKKVFVEDDRLTLVSLNKDY
                     DDLHFYRNESVRLIGKVIL"
     gene            3069..4001
                     /locus_tag="O0V33_002480"
     CDS             3069..4001
                     /locus_tag="O0V33_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCX8977076.1"
                     /translation="MKKYDIAVLDFETMNEHMNSPCEVAVSLIKDLSIVKVYSSYINP
                     PNNRYNLKNAKIHKIPEDVILKAPKYPDIYQEILYLLKESHLIIAHNALFDISVLKNT
                     NNYYDLPVPNFMYVDSINIFRSFHAISSFKLENLCSLYDIDKEKLHSAKFDVLALSKM
                     LISLAKNNQHYSVLKLIHYMPKQYIRFSKYSNSPTKLFDSGFQKIHMKISEINKIEVE
                     SVIPILKDKNVVFTGNFDTEKQDLMILNRKKGAYIRSDVTAKTDILVEGVQDDKYKDV
                     NGLVSKQRKAREYVGNGAKIQFLNEEDLINLIKE"
     gene            4007..>4339
                     /locus_tag="O0V33_002481"
     CDS             4007..>4339
                     /locus_tag="O0V33_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977077.1"
                     /translation="MIKKIFTKKHVFLVIEDENHNHSDAVFGKSILLSIYVGVNKKTN
                     SKSGKFIYLDRSKRIVRQSDITKIESANENDVDFYNLLKKEKEIVYSKNIVDKYNLAN
                     YIIYYEVST"
BASE COUNT         1454 a          689 c          662 g         1534 t
ORIGIN      
        1 cgaaaatgat tttccacatc ctttcaaaaa gttaataaga tggtctaatg gcattgcagc
       61 aggtgctgac attcaaatct ttgtaacaaa ctatataaac gagaacgatt tacatattca
      121 aaattcattg atagttgcta tagtttgttt attaagtttt ttatcgacct attgttcaca
      181 ttctaaaaag taataataag gcctaattta gttaaccttc tttaacaact ctgcaactgc
      241 tcgcaacaat tcagggttgt tacttctttc taaacagtaa ctagcatgct ttagtaattt
      301 gagttttaat ttattttttt ctttcgcgat tctaaatttt tgtaacattt gttatgcctc
      361 ctttgcattt ccaaaaattt aatctaactt aaattctttt ccatctatta atccataaaa
      421 gttatttttt aaatgcggat gtctttcaag cgtcatttca ataaaacgcg ggtctatcat
      481 taagtcgtag ccatcgttgt attgaatatt aacgggtcgt ctattaccgg cttcgtcata
      541 gtagtaataa atgacttttt tgttttgagc ttgcattgtt cgttcctcct attaagatgt
      601 ttgtttttct cctaaaaact tattaacaaa gtattgttgt cctttgcctg ttacttttgg
      661 cgtcttacta attgatgtgt gaccgtccga atgtgtgatt gatgtttctt taatttcgaa
      721 taactcacgt tccattgaat actgtgtagg catgttataa tccacaccct tgcgtttaat
      781 aaggaatccg ttttgacgta accactcaaa caatctgcgt tgcccgatgt ttataccgtt
      841 ttgtttaatg atctttgcta actctccaac taaaattgat gtcttagtag tagctactgc
      901 atctgcaaat acaatttttg gtttatcacg ttcaatcttt gtttctaatt gattgattgt
      961 gttgttagca atttttaaag cacgttgcat aatcatttct gggctattcc atgctttttc
     1021 aacttggatg aagtattgtc ttgcacgttt gccaggttca ctacgttgaa tcattgcaat
     1081 ctcttttgca gtgtctagtg tgagtgcgtg gtcagtttga ttctgacgac ctcctagtgg
     1141 gttatggaca aaaatgtccg tgactatata atcgatattt tcttcaaatc cgtaatcact
     1201 cattctttca aaccattttt tgtatggagt cttaacctct aatgcttgat gaagttctcg
     1261 accgctgatt gcgatttctc cattttcttt ttcttgtatg ttgaacattt cgcctatgtt
     1321 cgattttgtt tgtaatgctt gcatattgtt tgtgctcctt tctgctatac tcctattaag
     1381 gaggtgaatg acttatgact gatgaagcta aatttgtcct tttacaactt tattcaattt
     1441 accttgatag aattgacgaa ggtatgtcta aacgttctgc atcttatttc ggtagtgatg
     1501 aatcctcatt taacgctttc tttttaggtt ttaattttga agactatatc gatgcagttc
     1561 ttgaattaaa gcatagagat tttgtaattg cttctgctga agatggcggt tttcttgaga
     1621 tggctctttc tcgagaaggt atcgcctact cagaatcaga atccaaaaaa gattacaaaa
     1681 cacttatggg tttaattaga gatttgaaaa aattaataat ctaaaatcca atcatctgct
     1741 attaaatcgt ctgcgctagg ctgccacctt ccggcggcgg tttgtctttt tttcttatag
     1801 tgtctagata cgactaggca ttgataacgc tgcaaattag ttggtaatat cccatacaca
     1861 tcttgattct ctctccttat acttattcct ttctccatcg ctagcttcgt tgctacttga
     1921 atgttcattt gttattcctc ctattaagat gtttgttttt ctttaaatgc taaaataatt
     1981 gatttctttt tatcattcgt aaatacgaaa ttttcgtatt cattacctaa aaaaatatca
     2041 tcatatttaa cattaaaagc actcatatac ttagaaagta aactatcttt aatgtttgta
     2101 gagtcttttt ccatattttg aattgtacgt gatgagacct taaataaatc tcctaactct
     2161 ttttgagtca atccgtaatc agtcctcaac tcttttaatg ttttcatgtt gtcaccgcct
     2221 ttcgtaaacc taatataata cgaaattttc gtatcgtcaa cattaaatac gtttttttcg
     2281 taaaaaactt tactatgata tgaaaatttc gtataataag aaaaaaggag gtaagtaata
     2341 tgaacaaaga aagaaatatt attatagcca aaaacattag aaaatttctc aacgattcaa
     2401 atatgtctca aaagaaactt gctgaactca ttaacataaa accatctact ttaagcgatt
     2461 atttaaattt acgttccaac ccctctcacg gcgttataca aaggatagct gatgttttcg
     2521 aggttggtaa aagcgacata gatactacat acaaagacga taacgacatc acttccatat
     2581 acaacaaact cacacctccc cgccaagaaa acgtacttaa ctatgcaaat gaacaattgg
     2641 aagaacagaa ttctaaagga gataacgttg tagatattaa ttcatataaa caggagaaaa
     2701 ctccagttaa cgtcaatggt tgcgtctctg ctggtgtagg agaacgttta cacgatgaaa
     2761 cgctatttac tgaaatggtt aaaggaccta tccccacaca cgatttagcg ttaaaagtaa
     2821 atggtgattc tatggaacct atgtttaaag atggcgaaat catatttgtg gagaaaactc
     2881 acaatataaa gaatggacaa attggtatat tcatcattga agaagaagcg tacgttaaga
     2941 aagtctttgt tgaagatgat agattgactc tagtttcact aaataaagat tacgacgatc
     3001 tacactttta tagaaatgaa agtgtgaggt taattggaaa agttatttta taaaaggagc
     3061 acttgcaaat gaaaaaatat gatattgcag tcttagactt tgaaactatg aatgaacata
     3121 tgaacagccc ttgcgaagtt gctgtatctt taattaagga tttatcaata gtaaaagttt
     3181 attcatctta tattaatcct cctaataata gatataactt gaaaaacgct aaaatacata
     3241 aaatacctga agatgtcata ttaaaagcac ctaaatatcc agatatttac caagaaattc
     3301 tctatctttt aaaagaatca catttaatta ttgctcataa tgcacttttt gatatttcag
     3361 tattaaaaaa tactaataat tattatgact tacctgttcc aaacttcatg tatgtcgata
     3421 gtataaatat ctttagaagc ttccacgcaa tctctagttt taaattagaa aatttgtgta
     3481 gcttatatga tatcgataaa gaaaaattac attctgctaa atttgacgtg ctagctttat
     3541 cgaagatgtt gatatcactc gctaaaaaca atcagcatta tagtgtatta aaattaatac
     3601 attatatgcc taagcaatac attagattta gcaaatattc taactctcca actaaacttt
     3661 tcgattcagg atttcaaaaa attcatatga aaatatctga gattaataaa atagaagtgg
     3721 aaagtgtaat ccctatttta aaagataaaa atgttgtttt tacaggtaat tttgacactg
     3781 aaaaacaaga tttaatgata ttaaatagaa agaaaggagc ttatatcaga agtgacgtaa
     3841 ctgcaaaaac agatatttta gtcgaaggtg ttcaagatga taaatataaa gatgtgaacg
     3901 gactagtttc aaaacaacga aaagctcgag aatatgttgg aaatggtgca aaaattcaat
     3961 ttttaaatga agaagactta ataaatttaa taaaggaata ataacgatga tcaaaaaaat
     4021 ttttacaaaa aaacatgtat tcttagttat agaagatgaa aaccataatc acagtgatgc
     4081 tgtttttgga aaaagtatat tactttcaat ttacgtcggt gtgaataaaa agactaattc
     4141 taaatcaggg aaatttatat accttgacag atctaaaaga atcgttagac aatctgatat
     4201 caccaaaata gaatcagcta acgaaaatga tgtagatttt tataatttac tgaagaaaga
     4261 aaaggaaatt gtttattcca aaaatatagt agataaatac aatttagcga actatataat
     4321 ttactacgaa gttagtact
//
LOCUS       DAKSQE010000154         4324 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.156, whole genome shotgun sequence.
ACCESSION   DAKSQE010000154 DAKSQE010000000
VERSION     DAKSQE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.156"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            189..833
                     /locus_tag="O0V33_002482"
     CDS             189..833
                     /locus_tag="O0V33_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HCX8977078.1"
                     /translation="MKITYKYRGDLPLNTENNKNQNQSAKNSERRGMLKGCGGCLISF
                     IILIILLSACSMMFSNNDNSTSNQSSKTQLTQKDEDKSENMPEEKSETDKDLQSTEEV
                     PANENTENNQHEIDEITTTDQSDDEINTPNVAEEESQDDLKDDLKEKQQPSDHHQSTQ
                     PKTSPSTETNKQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDKRACEPDKY"
     gene            914..1909
                     /locus_tag="O0V33_002483"
     CDS             914..1909
                     /locus_tag="O0V33_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCX8977079.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQNQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGITGNLGGNSSKETNKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSLAPTNAKGIFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QSDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAVASSNSDNVSYEASATTPATTSSADTDSEDSEKSSKDED
                     KQNASKSDNSSVEKSESNEETAPVEPMPHSKPTTSEAPPSQNIHNEDSMYDASTE"
     gene            1985..2611
                     /locus_tag="O0V33_002484"
     CDS             1985..2611
                     /locus_tag="O0V33_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977080.1"
                     /translation="MKFKAIVAITLSLSLLTACGANQHKENSSKSNDTNKKTQQTDNT
                     TQSNTEKQMTPQEAEDIVRNDYKARGANENQTLNYKTNLERSNEHEYYVEHLVRDAVG
                     TPLKRCAIVNRHNGTIINIFDDMSEKDKEEFEAFKKRSPKYNPGMNDQAEMDNESEDI
                     QHHDIDNNKAIQNDLPDQKVDDKNDKNAVNKEEKHDNRENNSAETKVK"
     gene            2652..2996
                     /locus_tag="O0V33_002485"
     CDS             2652..2996
                     /locus_tag="O0V33_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HCX8977081.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIDNAEHTLFTPYM
                     METLSTLGVKPDIIDLIHKGTELEDFSAFNLSIDDTVTVCLQRTEELLKQYKNVEFND
                     KILINWRIMQEK"
     gene            3094..3666
                     /locus_tag="O0V33_002486"
     CDS             3094..3666
                     /locus_tag="O0V33_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977082.1"
                     /translation="MEKNEYTAKYNEYSQLLDATYSQAVAYLLNKYGAVTDDYYKEKS
                     YTRFLNGEIKSISKGKYTRASEGLYCHHISEDKFQNLSDLRFISEFKYSYNYQKKENL
                     VYCDLIEHLILHAIITKESNGQFGVAGLCQMIKPTVIDWYIGEYNPKPAWMQATKARA
                     YLPGILVEKLLIKIDDMLKGIEIYDFLESR"
     gene            complement(3815..>4324)
                     /locus_tag="O0V33_002487"
     CDS             complement(3815..>4324)
                     /locus_tag="O0V33_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HCX8977083.1"
                     /translation="KRELNQTQSAFQKLKITREEKLKNHKSRIKEDLKRIRNLTMKTE
                     HDMDSEKSNDYKEELEYLIKRILKDESVIKIDKEMEDLKKKKLYLDARLKKEEILTSE
                     YLRPEICKELREKLKQYHYVESELFPDVYRHIGYIQSNFLSNQTNNRTINKNNISENL
                     VDLLKLKEN"
BASE COUNT         1677 a          683 c          675 g         1289 t
ORIGIN      
        1 tttaagcagt aatacaactt tacaattatt aaattagcat tcctacaatc caaaaatatt
       61 tttaaagttt tgaaaaaaat aaagtatttc cactactaat ttggtaaaat atgattatgt
      121 gatatagtga aagcgtttta cacttaataa ctccctctta aatgcatcca ggttttatgt
      181 agtaaatcat gaaaataaca tataaatata gaggagattt acctttgaat acagagaaca
      241 acaagaatca aaaccaatct gctaaaaatt ctgaaagacg tggcatgtta aaaggatgcg
      301 gcggttgcct tatttctttt attatattaa ttatcttatt atcagcctgt tcaatgatgt
      361 ttagtaataa tgacaattcc actagtaatc aatcatcaaa aacgcaatta actcaaaaag
      421 acgaagataa aagtgaaaat atgcctgaag aaaaatcaga aacagataag gatttacaat
      481 caaccgaaga agtacccgca aatgaaaata ctgaaaataa tcaacatgaa attgatgaaa
      541 taacaacaac agatcaatca gatgatgaaa ttaacacacc aaacgttgca gaagaagaat
      601 cacaagatga cttgaaagat gatttaaaag aaaagcaaca accaagtgac catcatcaat
      661 ccacgcaacc taagacttca ccatcaactg aaacaaacaa gcaacaatca tttgctaatt
      721 gtaagcaact tagacaagta tatccgaatg gtgtcactgc cgatcatcca gcatatcgac
      781 cacatttaga tagagataaa gataaacgtg catgtgaacc tgataaatat taaacaacaa
      841 gcgaattgaa ttcaaattgt atttagcttt atgcactaat cacatagtaa ataatgaggg
      901 agatttttta ggcatgagca atcaattcaa aagcgaagaa gagcgaagac aatgggaaca
      961 attccaagct ttccaaaatc aacaaaacca acagaaccag caatacggac aaaagaaatc
     1021 taaaaaagga tggttctggg gctgtggtgg ttgtctagta ttattcattt taattatcat
     1081 cggtatttca gcttgtacag ctggtattac aggtaacctt ggcggaaata gttctaaaga
     1141 aacgaacaaa acccataaaa tcggtgaaac tgttaaaaat ggcgaccttg aagtcactgt
     1201 aaattcagtg gaaactatga aatctgtagg accatctctt gcaccaacaa acgctaaagg
     1261 tatatttgtc gttgctgatg tgacgattaa aaacaaaggt aaagaagcgt taacaattga
     1321 tagttcaatg tttaagctaa aatccggtga taaaacattt gaagcagata atacaggttc
     1381 aatgtctgct aatcaaagtg acaatggtag tatagaaaat tcatttttct tacagcgtat
     1441 aaatccagat agcactgctc aaggtaaaat tgttttcgat gtgtcagaaa acatagccaa
     1501 cgcaaaagat aaaaaattag aagttatttc tagtttattt agcgtcaaga agattacatt
     1561 tgatttatcc gatgctaaaa aaacatcaaa agctaaaaaa gacaagcaag atacagaagt
     1621 agctgttgcg agttcaaata gcgataatgt aagttatgaa gcttcggcta ctacacctgc
     1681 tacaacttct agtgcggata ctgattctga agatagcgaa aagtctagta aagatgagga
     1741 taagcagaat gcgtctaaaa gtgataattc tagtgtagaa aaaagtgaat ctaatgagga
     1801 aactgctcct gtagagccca tgccccatag caaacctacc actagtgaag cacctcctag
     1861 ccaaaatatt cacaatgaag atagcatgta cgacgcttca acagaataaa atttcagggt
     1921 agctcggcta cccttctttt acggaaaaat taattataca taatcaaatc aaggagataa
     1981 aaaaatgaaa ttcaaagcta tcgttgcaat cacattatca ttgtcactat taaccgcctg
     2041 tggtgctaat caacataaag aaaatagtag taaatcaaat gacactaata aaaagacgca
     2101 acaaactgat aacactacac agtcaaatac agaaaagcaa atgactccac aagaagccga
     2161 agatatcgtt cgaaacgatt acaaagcaag aggtgctaac gaaaatcaaa cattaaatta
     2221 taaaacaaat cttgaacgaa gtaatgaaca tgaatattat gttgaacatc tagtccgcga
     2281 tgcagttggc acaccattaa aacgttgcgc tattgttaat cgacacaatg gtacgattat
     2341 taatattttt gatgatatgt cagaaaaaga taaagaagaa tttgaagcat ttaaaaagag
     2401 aagccctaaa tacaacccag gtatgaatga tcaagctgaa atggataatg agtcggaaga
     2461 cattcaacat catgatattg acaataacaa agccattcaa aatgacttac cagatcaaaa
     2521 agtcgatgat aaaaacgata aaaatgctgt taataaagaa gaaaaacacg ataaccgtga
     2581 aaataattca gcagaaacta aagttaaata agggtatact ttgattaatc gtaattttta
     2641 ggagaaccgc tatgataaca tttgaaaata tacaacaact tgaaaaatac accttaatga
     2701 ctatgcatgg tctttttaat caacttaaac tcggtattat ttcaatcgac aatgcagagc
     2761 atacgctctt tacaccttat atgatggaaa cactctctac cctaggtgtg aaaccagaca
     2821 ttatcgattt aattcacaaa gggacagagt tagaagactt ttcagcattt aatttatcaa
     2881 ttgatgatac agttacagtc tgtttacaaa gaactgaaga actattaaaa caatacaaaa
     2941 atgtagaatt caatgacaaa atattaatca attggcgcat tatgcaagag aaatagacat
     3001 ataaaagtag aacctaacta tgtggatatt gattttattc tttctaaatt acaagtattt
     3061 cattttataa agtttgaaaa gaggtttatt gaaatggaga aaaacgaata tacagctaaa
     3121 tataatgaat atagtcaatt attagacgct acttactcgc aagctgtagc atacctttta
     3181 aataaatatg gcgctgtaac cgatgactat tataaggaaa aatcatacac acgattttta
     3241 aatggagaaa tcaaaagtat ttcaaaagga aaatacacta gagctagcga aggattatat
     3301 tgtcatcaca taagcgagga caaattccaa aacttatctg acctaagatt catttccgaa
     3361 tttaagtact catacaatta tcaaaagaaa gaaaacttag tttactgcga tttaatcgag
     3421 catttaattt tacatgcaat tattacaaaa gaatccaatg gccaatttgg tgtggctgga
     3481 ttatgtcaaa tgatcaaacc aacagtcatt gattggtaca ttggcgaata taatccaaaa
     3541 ccagcatgga tgcaagccac caaagcacgt gcctatttgc ctggaatatt agtagagaaa
     3601 ttactcatta aaattgacga tatgttaaaa ggcatagaaa tatatgattt ccttgagtct
     3661 agataaatga ttaatggaga tttatttttt gctgttgaga ttttgttata gatgtttaaa
     3721 cctgtaatta aatatatttt ataaaaatag accacgcata cctctctata aacggacaat
     3781 gtttataaat gagtttgcat ggtcttgaat tgtattagtt ttcttttagt tttaataaat
     3841 cgactagatt ttctgatata ttatttttat taatagtacg attatttgtt tgatttgata
     3901 gaaaattaga ttgaatataa cctatatgtc tgtatacgtc agggaataat tcactttcaa
     3961 cataatgata ctgttttaat ttctccctta attctttaca aatctcaggc cttaaatatt
     4021 cactagtcaa gatttcctct tttttcaacc ttgcatctaa atatagtttt ttctttttta
     4081 agtcttccat ttctttatct attttaataa cgctttcatc ttttaagatt cgtttaatta
     4141 aatattccaa ttcctctttg taatcattag atttttcact atccatgtca tgttcagttt
     4201 tcatagttaa attgcgtatt cttttgaggt cttctttaat tctactctta tgatttttca
     4261 atttctcttc tctagttatt tttagttttt gaaaagcaga ttgcgtttga tttaactccc
     4321 tttt
//
LOCUS       DAKSQE010000155         4306 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.157, whole genome shotgun sequence.
ACCESSION   DAKSQE010000155 DAKSQE010000000
VERSION     DAKSQE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.157"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(205..1407)
                     /locus_tag="O0V33_002488"
     CDS             complement(205..1407)
                     /locus_tag="O0V33_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HCX8977084.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
     gene            1725..2795
                     /locus_tag="O0V33_002489"
     CDS             1725..2795
                     /locus_tag="O0V33_002489"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCX8977085.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITTEIKSTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSIGISKCSNEVELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            2810..4168
                     /locus_tag="O0V33_002490"
     CDS             2810..4168
                     /locus_tag="O0V33_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCX8977086.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIP
                     FEGENVDGHRFVSKALQDGAGAAFYQKGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCCVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLTYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLSTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIGVLTSDLKAHDRVLVKGSRGMKLEEVVNAL
                     IS"
BASE COUNT         1602 a          620 c          766 g         1318 t
ORIGIN      
        1 atatcaaaaa agctaatttc tatcaaataa ttaatagaaa ttagcttttt tacattgcct
       61 aagaacttaa tgtctcaagc acttcaaaga gggattgtta ttatcgagta atatctatat
      121 atgacatttt aaatcgacga cgcaaataga ctatataacg tcatcttgaa aagttatgat
      181 atttgaaact caaaatagtt taaattaatt actttttgga tggtataaat ctacatatcg
      241 ttttggttca tgataataaa ttgataagac tatacctatt ccagtcatca tactccatag
      301 cgcactacca ccataactaa taaatggtaa tggaatacca gtgattggta acaactgaat
      361 tgtcatacca atattttgta aaatatggaa cacaagtaaa gtgacgaaac caacgataaa
      421 gattttgtta aattgatctt caattttcgc agctaatctt attagatgga agattaaaaa
      481 taaaaatatt aagatcaata tgacagaacc gataaaacca agttcctctc caatcactga
      541 aaagataaag tcagtatgat tttcaggtat ataaacctca ccgtgattgt atcctttacc
      601 tagtaactgt cctgaaccga tagctttaag tgattcagtt aaatgatagc catcaccact
      661 actatatgta taggggtcaa gccatgaatt gattcgtccc atttgataca gttggacacc
      721 taataaattt tcaattaatg cgggtgcata tagaatacct aaaatgactg tcattgcacc
      781 aacaatacct gtaataaaga taggtgctaa gatacgccat gttataccac ttactaacat
      841 cacacctgca ataattgctg ctaatactaa tgtagttcct aggtcatttt gcagtaatat
      901 taaaatactt ggtactaacg agacaccaat aattttgaaa aataataaca aatcactttg
      961 gaatgattta ttaaatgtga attgattatg tctagaaacg acacgcgcta atgctaaaat
     1021 taaaataatt ttcatgaatt cagatggctg aatactgata gggccaaacg tgtaccaact
     1081 tttggcacca ttgataatag gtgtaatagg tgactcagga ataacgagca atcctattaa
     1141 taatagacag attaagaaat acaataaata tgtataatgt ttaatctttt taggtgaaat
     1201 aaacatgatg atacctgcaa aaattgcacc taaaatgtaa taaaaaattt gtctgatacc
     1261 gaaattagca ctgtattgac caccgcccat tgccgagtta ataagcagaa cactgaaaat
     1321 tgctaaaaca gctatagtgg ctactaatac ccagtctact ttgcgaagcc aatgcttatc
     1381 cggctgttga cgagatgaat aattcattgc aaactccttt tatactcact aatgtttata
     1441 tcaattttac atgacttttt aaaaattagc tagaatatca cagtgatatc agctatagat
     1501 ttcaatttga attaggaata aaatagaagg gaatattgtt ctgattataa atgaatcaac
     1561 atagatacag acacataacg cctcgttttt aaaatgcaaa acagcattaa aatgtgatac
     1621 tattaagatt caaagatgcg aataaatcat ttaacaattg gactaaatca atattaattt
     1681 atattaaggt agcaaaccct gatatatcat tggaggaaaa cgaaatgaca aaagaaaata
     1741 tttgtatcgt ttttggaggg aaaagtgcag aacacgaagt atcgattctg acagcacaaa
     1801 atgtattaaa tgcaatagat aaagacaaat atcatgttga tatcatttat attaccaatg
     1861 atggtgattg gagaaagcaa aataatatta caactgaaat taaatctact gatgagcttc
     1921 atttagaaaa tggagaggcg cttgagattt cacagctact gaaagaaagt agttcaggac
     1981 aaccatacga tgcagtattc ccattattac atggtcctaa tggtgaagat ggcacgattc
     2041 aagggctttt tgaagtattg gatgtaccat atgtaggaaa tggtgtattg tcagctgcaa
     2101 gttctatgga caaacttgta atgaaacaat tatttgaaca tcgagggtta ccacagttac
     2161 cttatattag tttcttacgt tctgaatatg aaaaatatga acataacatt ttaaaattag
     2221 taaatgataa attaaattac ccagtctttg ttaaacctgc taacttaggg tcaagtatag
     2281 gtatcagtaa atgtagtaat gaagtggaac ttaaagaagg tattaaagaa gcattccaat
     2341 ttgaccgtaa gcttgttatt gaacaaggcg ttaacgcacg tgaaattgaa gtagcagttt
     2401 taggaaatga ctatcctgaa gcgacatggc caggtgaagt cgtaaaagat gtcgcgtttt
     2461 acgattacaa atcaaaatat aaagatggta aggttcaatt acaaattcca gctgacttag
     2521 acgaagatgt tcaattaacg cttagaaata tggcattaga ggcattcaaa gcgacagatt
     2581 gttctggttt agtccgtgcg gatttctttg taacagaaga caaccaaata tatattaatg
     2641 aaacaaatgc aatgcctgga tttacggctt tcagtatgta tccaaaatta tgggaaaata
     2701 tgggcttatc ttatccagaa ttgattacaa aacttatcga gcttgctaaa gaacgtcacc
     2761 aggataaaca gaaaaataaa tacaaaattg actaactgag gttgttatta tgattaatgt
     2821 tacattaaag caaattcaat catggattcc ttgtgaaatt gaagatcaat ttttaaatca
     2881 agagataaat ggagtcacaa ttgattcacg agcaatttct aaaaatatgt tatttatacc
     2941 atttgaaggt gaaaatgttg acggtcatcg ctttgtctct aaggcattac aagatggtgc
     3001 tggggctgct ttttatcaaa aaggcacacc tatagatgaa aatgtaagcg ggcctattat
     3061 atgggttgaa gacacattaa cggcattaca acaattggca caagcttact tgagacatgt
     3121 aaaccctaaa gtaattgccg tcacagggtc taatggtaaa acaacgacta aagatatgat
     3181 tgaaagtgta ttgcataccg aatttaaagt taagaaaacg caaggtaatt acaataatga
     3241 aattggttta cctttaacta ttttggaatt agataatgat actgaaatat caatattgga
     3301 gatggggatg tcaggtttcc atgaaattga atttctgtca aacctcgctc aaccagatat
     3361 tgcagtcata actaatattg gtgagtcaca tatgcaagat ttaggttcgc gtgaggggat
     3421 tgctaaagct aaatctgaaa ttacaatagg tctaaaagat aatggtacgt ttatatatga
     3481 tggcgatgaa ccattattaa aaccgcatgt taaagaagtt gaaaatgcaa aatgtattag
     3541 tattggtgtt gctactgata atgcattagt ttgttgtgtt gatgatagag atactacagg
     3601 tatttcattt acgattaata ataaagaaca ttatgatctg ccaatattag gaaagcataa
     3661 tatgaaaaat gcgacgattg ccattgcggt tggtcatgaa ttaggtttga catataacac
     3721 aatctatcaa aatttaaaaa atgtcagctt aactggtatg cgtatggagc aacatacatt
     3781 agaaaatgat attactgtga taaatgatgc ctataatgca agtcctacaa gtatgagagc
     3841 agctattgat acactgagta ccttgacagg gcgtcgcatt ctaattttag gagatgtttt
     3901 agaattaggt gaaaatagca aagaaatgca tatcggtgta ggtaattatt tagaagaaaa
     3961 gcatatagat gtgttgtata cgtttggtaa tgaagcgaag tatatttatg attcgggcca
     4021 gcaacatgtc gaaaaagcac aacacttcaa ttctaaagac gatatgatag gagttttaac
     4081 aagcgattta aaagcgcatg accgtgtatt agttaaaggc tcacgtggta tgaagttaga
     4141 agaagttgta aatgctttaa tttcatagag attagtcgag cgacctttta cttataaaaa
     4201 tgatttgaat taatactaaa agattacaaa gatgaggtgg ttttgtgtgt aaatacaaaa
     4261 ttgccttttt ctttttatgt taaatctata aatttgaaac taaatc
//
LOCUS       DAKSQE010000156         4160 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.158, whole genome shotgun sequence.
ACCESSION   DAKSQE010000156 DAKSQE010000000
VERSION     DAKSQE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.158"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..446)
                     /locus_tag="O0V33_002491"
     CDS             complement(<1..446)
                     /locus_tag="O0V33_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCX8977087.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPK"
     gene            complement(739..2028)
                     /locus_tag="O0V33_002492"
     CDS             complement(739..2028)
                     /locus_tag="O0V33_002492"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCX8977088.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHSNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIKQTLKAADYAFSQMK"
     gene            2109..2564
                     /locus_tag="O0V33_002493"
     CDS             2109..2564
                     /locus_tag="O0V33_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCX8977089.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIVYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKYGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            2570..3520
                     /locus_tag="O0V33_002494"
     CDS             2570..3520
                     /locus_tag="O0V33_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCX8977090.1"
                     /translation="MKVVGLNRMREVETELQQRFSDVDFKFYKKASEIPESDLANLDI
                     LVGYDGGINEAFLRRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIVINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKEALLIEVLKSRVIRHAYLDVFENEPLKPNHELYELDNVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNGLIENEVDAKKGY"
     gene            3617..4063
                     /gene="perR"
                     /locus_tag="O0V33_002495"
     CDS             3617..4063
                     /gene="perR"
                     /locus_tag="O0V33_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HCX8977091.1"
                     /translation="MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
BASE COUNT         1527 a          630 c          687 g         1316 t
ORIGIN      
        1 aatttaggtg gtagaaacac aaagttaact agagatgaac ttatgacacc aatcattaca
       61 agtacaaacc tgtaaaaggc agtaatatag aaagaaccgg tatgttgtcc cataatgatt
      121 aatgctgtta cactcgccaa agtagcaaca tgtgctaaat taaatttaaa taaaatagca
      181 ataagtacta tgacggtaac acccataatg ataaaattat cactaaaaat tgttaccatt
      241 gtaacagata gtatggcacc tataatgtta cccaatgctt gatcagaaac tgttttaaat
      301 gaacgataaa tactaggttg cattgcaaca acagcactga caccagctaa ggctttcaga
      361 ccaacatcat ccggtagtaa agaagcgata gacatagcta aaataatggc tataccagtt
      421 ttgaaaatcc gagctcctag tctcaaaaat aatgacgccc cttttaagtt tattgaatat
      481 ctaacattcg tattcattac tgttatacac ttactagtta caaaattcaa acttatttat
      541 agttgttaaa ataaatcata cataatactg atagcgatgt aaaactttag tcagagatta
      601 aaatagtata aatttgtaaa ataaaaactc acatagggac atatcaattt aaacgttaat
      661 agttaacgat ataaaatgaa tctactatgt gagcatttgt tttattttaa ttcaatttaa
      721 aatatatttc cttaaaagtt atttcatttg actaaaagca tagtctgcag cttttaaagt
      781 ttgtttaata tcttcttctg tatgttcagt tgttaagaac caagcttcaa acttagaagg
      841 tgctaaattg ataccttgat ttaacatcaa tttgaaaaat ttaccgaacg cttcgccgtc
      901 agaatgttca acttgatcat aatgtgtgac tttttcatct gtaaagtaca atgttaaaga
      961 tccgtaaata cgattaattg tagctgtgat attatgtttt tcgattaatt taagtaaacc
     1021 ttcttctagt tgttggccta agctgtctaa tttttcataa acaccgtctt gttctagtac
     1081 ttcgagtaat gcaatacctg ctttcataga taacggatta ccagccattg taccagcttg
     1141 atatgcaggt cctagaggtg ctacttgttc cataatatct tgacgtccac catagcctcc
     1201 aattggtaaa ccaccgccaa caattttacc aaatgcagtt aaatcaggga taacacctaa
     1261 taaatcttga gcggcaccgt aatggaaacg gaatgcagta attacttcat cataaatcac
     1321 tagtgtcccg ttgctatgtg aaatttcatt aacctcttct aaaaatccag gttgaggcat
     1381 taccattcca aagttaccaa caattggttc tactaatact gcggcaattt catcacccca
     1441 aaattcaatt gcttctttat atgcgttaat atcattgaaa ggaacagtaa tgacttcacg
     1501 tgctacgctt tctggaacac cagctgaatc tggagaaccg agttgagatg ggccgctacc
     1561 tgctgcaacc aatactaaat cagaatggcc atgataagat ccagcaaatt ttataatttt
     1621 atttctttta gtatatgcac gtgcaacacg aattgttgtc atgactgctt ctgttccaga
     1681 atttacaaag cgaattttct caagagatgg aattgcatca cgtaattttt tgctgaattc
     1741 aatttctagt tcagtcggtg taccaaataa aacaccttta gcagcttgtt cttgaattgc
     1801 tttagtaata tgaggatgtg catgccccgt aataattgga ccgtatgctt gaagataatc
     1861 aataaattta ttgccatcga catcatataa atatgcaccg tgtccttctt tcataacaac
     1921 aggtgcaccg cctcctacag ctttataaga acgagaaggg gaattgacac cgcctagaat
     1981 atattcgttt gaaagttgtt gtaaacgttc actttcacta aaattcattt atatcaacct
     2041 cttttaattt aatattttca tctaatatcg tatcataaaa ttattataat gaagaaaaag
     2101 gtgattatat gttgcaaaaa ggagaacaat ttccaatatt taaattagaa aatcaggacg
     2161 gaactgtcat tacaaatgat acattaaaag gtaaaaaggc gattgtatat ttttatccta
     2221 gagataatac acctacttgc accacagaag cttgtgactt tagagacaat ttagaaatgt
     2281 tcaatgattt agatgttgca gtatatggta taagcggtga ttcaaagaaa aaacaccaaa
     2341 attttattga aaaatacgga ttgaatttcg atttattagt agatgaagat tttaaattag
     2401 ctaaagaaac tggcgtatat cagttaaaaa aatcatttgg caaagaaagt atgggcattg
     2461 taagaacgac ttttataata gatgaacaag gtaaagtatt agatgttatc gagaaggtta
     2521 aagttaaaac acaaatagaa gaacttaaaa acattttggg gtgacatata tgaaagttgt
     2581 tgggttgaat cgtatgcgtg aagttgaaac tgaattacaa caacgctttt cagatgtaga
     2641 ttttaaattt tataaaaaag catcagaaat acctgagagc gacttggcta atttagatat
     2701 attagttggt tatgatggcg gtatcaatga ggcattttta cgacgttgtc caaatttaaa
     2761 atggatcgca tggtttgcaa cgggtgtaaa tacattaccg ttagattata ttgcagatca
     2821 cggcatactt ttaactaatg gaaaaggtgt tcaagctaaa caattatctg aatacatttt
     2881 ggctttcatt ttagatgatt ataaaaagat gaaactatca tatgataacc aacgacaaca
     2941 tatatatgat tcgaaaataa ctggtaaacg cctatcagga caaacagttt tatttttagg
     3001 tacaggtgca attgctacta gaactgcgaa gttagcaaag gcttttaata tgaatttaat
     3061 tggtctgagc aagtcaggtc agaacaaaga tgagtttgat gagatatata ctatcgaatc
     3121 acttgaaagc acattaccaa atgctgacat tgttataaat gctttaccag aaacgcaaga
     3181 aacgattcat ttactaaaga aaaaacattt tgaattaatg aaagatgaag cactttttat
     3241 aaatatagga cgaggtagca tagttaaaga agcgctctta atagaagtat taaaaagtag
     3301 agttattcga catgcatatt tagatgtgtt tgaaaatgaa cctttgaaac ctaatcatga
     3361 attatatgaa ttggataatg taactataac agcgcatata actggtaatg attatgaagc
     3421 aaagtatgac ttattagata tttttaaaaa caatctagtt aattttctca ataagaatgg
     3481 tctaattgag aatgaagttg atgctaaaaa aggctattaa atgaaatcat catgtaaata
     3541 ttgacacgcg cgcaatacta cagttatatt tatagtaagt aataataatt attatataag
     3601 aaagatggtg atatagatga gtgttgaaat agaatcaatt gaacatgaac tagaagaatc
     3661 aattgcatca ttgcgacaag caggcgtaag aattacacct caaagacaag caatattacg
     3721 ttatttaatt tcttcacata ctcatccaac agctgatgaa atttatcaag cactttcacc
     3781 tgattttcca aatataagtg ttgcgacaat atataataac ttaagagtgt ttaaagatat
     3841 tggaattgta aaagaattaa catatggaga ctcatcaagt cgattcgact ttaatacaca
     3901 taatcattat catattatat gtgaacaatg tggtaagatt gttgattttc aatatccaca
     3961 gttaaatgaa attgaaagat tagctcagca tatgactgac tttgacgtaa cacatcatcg
     4021 aatggaaatt tatggagttt gtaaagaatg ccaagataaa taatttaact ttggtagtat
     4081 gacaaattaa aaaggcgtta gcttacttca tataagtaag cgtaatattt aagaagttaa
     4141 acgacatgaa agttgtttaa
//
LOCUS       DAKSQE010000157         4127 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.159, whole genome shotgun sequence.
ACCESSION   DAKSQE010000157 DAKSQE010000000
VERSION     DAKSQE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.159"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(166..1884)
                     /gene="ptsP"
                     /locus_tag="O0V33_002496"
     CDS             complement(166..1884)
                     /gene="ptsP"
                     /locus_tag="O0V33_002496"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCX8977092.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNNAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ASALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEAKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMDGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
     gene            complement(1887..2153)
                     /locus_tag="O0V33_002497"
     CDS             complement(1887..2153)
                     /locus_tag="O0V33_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCX8977093.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            complement(2307..2849)
                     /locus_tag="O0V33_002498"
     CDS             complement(2307..2849)
                     /locus_tag="O0V33_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HCX8977094.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRER
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISSAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            complement(2903..4075)
                     /locus_tag="O0V33_002499"
     CDS             complement(2903..4075)
                     /locus_tag="O0V33_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCX8977095.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQEVNTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHV
                     LNLFSYTGAFSVIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRIFSVQKDYDKLITGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
BASE COUNT         1283 a          791 c          553 g         1500 t
ORIGIN      
        1 taattaaaga acaatattta tgtcaatgac acctagttgt ttaattagtt agcaacaaca
       61 ttccattgaa attaacataa atcaaaaaaa gaacccgaag tattcataat tactcacaat
      121 gaatacttcg ggtttattta taattctgtc tttcgaaaga attaattatt ttacgtagtt
      181 gttaactaat tcaataactt cttcttgcgt tgcgcagtcg actgcacggt tagcaagttc
      241 agtcatttca tttttactta aaccattaat ttgtcttctt gctttcagaa tagacgttgc
      301 actcatagag aactcatcta aacctaaacc aagcaataat ggaatagctg tttcatctcc
      361 agccatttca ccacacatac ctgtccattt accttcttta tgtgacgctt caataacttg
      421 tttaactaaa cgtaagattg aaggattata tggttggtat agatatgata cacgctctga
      481 catacggtca gcagctaatg tgtattgaat taaatcattt gtaccgatac tgaagaaatc
      541 tacttcttta gcaaagacat cagctaatgc tgctgttgca ggtatctcta ccatgattcc
      601 taattctata tcatctgaaa tgtcatgacc ttcattttta aggttttctt tttcttctaa
      661 taatatagct ttagcttctc taaattcgtt aattgttgca accattggga acatgatatt
      721 taacttacca taaactgatg cacgtaataa tgcacgtagc tgtggtctga aaatatcttg
      781 ttgcgcaagg cataaacgaa tcgcacggta acctaagaat ggattcattt cttcaggcaa
      841 gtttaagtat gataattctt tatctccacc tatatctaaa gtacgtacaa caacacgttt
      901 accgtccatt gcttctaata cttctttata agcttcaaat tgttcttctt ctgtaggcat
      961 ttggtcacga cccatatata aaaactcagt tctatataag ccgatacctt gtgcaccatt
     1021 ttcaataaca cctggcaaat cattaggtgt accaatattt gcagcaagct ctgcgtgaac
     1081 accatcaact gtaacagtat cagcatcacg tagtttttgt aattctttct tgtcagcaaa
     1141 ataacgctca cgtttatctt gataagcgat taactcatct tcagttggat taacgattac
     1201 atcaccattt aatccatcta cgataatcat gtcgccttgt ttagcttctt gagtaattga
     1261 ttttgtacca acaattgctg gaatttctaa agaacgactc ataatcgcag agtgacttgt
     1321 tcttccgcca atgtttgtag caaaaccttg tacgaattct ttatttaatt gagcagtatc
     1381 tgatggcgtt aagtcattcc ctacaataac aacgctttca tcaatcatac tcggattcgg
     1441 taattctaca cctaaaatat gtgataacac acgtttagaa acgtcgcgaa tatcagccgc
     1501 acgttctttc atgtattcat tatccataga ttcaaaaatc gtaacaaatt gtgttgttac
     1561 atctgttaat gctgaagcag cattagcgtt ttcattttta atcttatctt gaattggttg
     1621 aattaattca gggtcatcta aaactaataa atgtgcatca aagatagcag ctttatcagc
     1681 acctaattga acctctgcat tatttctaat tttagttaac tcaactttag aagcttcgat
     1741 agcgttattg aacttagcaa cttctccttc aacatcagtg actttttcat ttttgtcgaa
     1801 tgttaagtct ggctcaacta acaaataagc tttagcaatt gcaacaccat cagatgcagc
     1861 aattccttta attaatttag acatgattat ttagtcaatc cttcttttga taagacgtca
     1921 ctgattgctt gaatggcgtc agattcatca ctaccgtcag cataaattgt aatttcagca
     1981 tctttaccaa caccaaggct cataacaccc atgattgatt ttaagttaac ctttttaccg
     2041 ttatattcta attggatatc agaatcgaat tttgaagctg tttgaactaa cattgttgct
     2101 ggtctagcgt gaataccagt ctcgtcgatg attacatatg aattttgttc cataattaca
     2161 tttctccttc gtatagttag aatcttcaat attattacaa tactaactta gttttatacc
     2221 ttaactttac caaaaccatg ctttaaattc aattcgttta aaaccggttt cagtaaaatt
     2281 tgcaaattgc aaaactatta tagcacctac atgacatttt taataacatt ttcgcatttt
     2341 tgaatgtgat cttttccaat tttattcata aaatagtaac taatagatgc agacacagct
     2401 tgtcctacaa caggaaacca ttttgtttgt ttagctgcag ttcgttttgc tacatctcta
     2461 attacaattt ttaaaaatgc acttgaaatt cttttaccaa taaattgact accttgaatt
     2521 gccgctgcag acataattct ttctttcaca tcatccccaa ggctattaac ttgcttatga
     2581 tctaatccgt atattttatt tacatcttcg ataatatctt tcattaattt taaatcaaca
     2641 ccaaagtcta atccaggaat tggaacaacg ctcattgttg atgacattaa agactttctc
     2701 tttacaattg cttcagcacg ttcgcgtctt tctctcaatt cttgaatcgt cgttggcatt
     2761 gcaccttttc cgtcaacatt ttctatttta aaaactgaat taccaatttt atttgttaaa
     2821 tttgatgtta aattattttt gaatcccatt gtatcatcac ccttcttttt ctcaataaga
     2881 tactataccc ctttttatca aattaatgtc taatatttac aaaaacagct tttaaatatt
     2941 tagatggctt ataatgtgga tgtgttttaa aatcttttgg taatcccata acttcagtta
     3001 attcataatc aacgccactc tcttcaagag tctttttaat agtattttta aattgcttta
     3061 atggatatac acttgcgttt gtacataata ataatgttcc ttcagatgat aagatattta
     3121 aggcgccagt aattaatttg tcataatctt tttgcactga aaatatacgt tttttattgc
     3181 gtgcaaagct aggtggatca atcacgatcg tgtcataact atgtccatga cgtgcagcat
     3241 atttatagaa atcaaaagtg tccatgacat aaatatattg agatttagga tcaatagcat
     3301 ttaatccaaa attttcttca gttaaactac gagaacgatt agccaaatct acacttgttg
     3361 ttgaagatgc ctcacttgct gctattacag aaaaagcacc tgtataacta aataagttta
     3421 aaacatggcg ttctttggca tattgatccc ttaatttctt gcgcacttct ttttgatcta
     3481 aaaagatacc tgtcatcaaa ccatcttcaa ggtctacatt ataaaatgtg aagttttctt
     3541 cgataacaat tggaaactca ggtgcatcgc cttcaacaaa accaccacta tattcgctgt
     3601 ctttaaatct tactttttcg tatatagatt tataatcaaa tacctttcta accgcttcaa
     3661 gaatggcata tttaaattta taaatacctt ttgagtacca ttgaatcaac aaatgaccat
     3721 cgtaattgtc gatcgttaat cccccaactc catcaccttc agcattaaat aatctaaaag
     3781 catttgttcc atctatatta aaataataat cacgttctgc taatgcagta ttaaacaatt
     3841 tcacaaagaa agctgtgttg acttcttgag ctttatcgta tgttagaacc caacctaatc
     3901 ctttatgttg gcgaccaaca taagccgttg caacatattg tgatttatca gtcacaattt
     3961 gaaaaatatc tccttctttt aaatgatctt gtgaatagat atcctcttct tcaattaaag
     4021 gatatccatt aaaatatttt gtttctttgc ctttgttcag agttgctatt ttcatctgac
     4081 aacttccttt ctgtgtttcg ttgtagtttt atatagatta aattgtt
//
LOCUS       DAKSQE010000158         4116 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.160, whole genome shotgun sequence.
ACCESSION   DAKSQE010000158 DAKSQE010000000
VERSION     DAKSQE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.160"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            322..720
                     /gene="nrdI"
                     /locus_tag="O0V33_002500"
     CDS             322..720
                     /gene="nrdI"
                     /locus_tag="O0V33_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCX8977096.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
     gene            683..2788
                     /gene="nrdE"
                     /locus_tag="O0V33_002501"
     CDS             683..2788
                     /gene="nrdE"
                     /locus_tag="O0V33_002501"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCX8977097.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            2908..3879
                     /gene="nrdF"
                     /locus_tag="O0V33_002502"
     CDS             2908..3879
                     /gene="nrdF"
                     /locus_tag="O0V33_002502"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCX8977098.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
BASE COUNT         1519 a          575 c          724 g         1298 t
ORIGIN      
        1 gaaattacat ttaaaagtaa tttaacacag aaatttaata gttattatca atgaatagtc
       61 atatttttta gaaaatgtac tgagcaaatg gaaggtatct aatgatctaa acactacata
      121 tagtgatttt tatacattca acccatataa gctactattt tctcaaatat aaatctatgc
      181 aattggttta catttgagaa aataagtagc ttcattatag ttaatacaat gctgagataa
      241 ccatagtagc gacgttgtta aagcattttt taattgtaat gactacttaa tttaaaaggg
      301 ttgaagaaag aaggtgatcc aatgaaaata atatattttt catttactgg aaatgtccgt
      361 cgtttcatta agagaacaga acttgaaaat acgcttgaga ttacagcaga aaattgtatg
      421 gaaccagttc atgaaccgtt tattatcgtt actggcacta ttggatttgg agaagtacca
      481 gaacccgttc aatctttttt agaagttaat catcaataca tcagaggtgt ggcagctagc
      541 ggtaatcgaa attggggact aaatttcgca aaagcgggtc gcacgatatc agaagagtat
      601 aatgtccctt tattaatgaa gtttgagtta catggaaaaa acaaagacgt tattgaattt
      661 aagaacaagg tgggtaattt taatgaaaac catggaagag aaaaagtaca atcatattga
      721 attaaataat gaggtcacta aacgaagaga agatggattc tttagtttag aaaaagacca
      781 agaagcttta gtagcttatt tagaagaagt aaaagataaa acaatcttct tcgacactga
      841 aatcgagcgt ttacgttatt tagtagacaa cgatttttat ttcaatgtgt ttgatattta
      901 tagtgaagcg gatctaattg aaatcactga ttatgcaaaa tcaatcccgt ttaattttgc
      961 aagttatatg tcagctagta agtttttcaa agattacgct ttgaaaacaa atgataaaag
     1021 tcaatattta gaagactata atcaacacgt tgcaattgtt gctttatacc tagcaaatgg
     1081 taataaagca caagctaaac aatttatttc tgctatggtt gaacaaagat atcaaccagc
     1141 gacaccaaca tttttaaacg ccggccgtgc gcgtcgtggt gagctagtgt catgtttctt
     1201 attagaagtg gatgacagct taaattcaat taactttatt gattcaactg caaaacaatt
     1261 aagtaaaatt ggtggcggcg tagcaattaa cttatctaaa ttgcgtgcac gtggtgaagc
     1321 gattaaagga attaaaggcg tagcgaaagg tgttttacct attgctaagt cacttgaagg
     1381 tggctttagc tatgcagatc aactaggtca acgccctggt gctggtgctg tgtacttaaa
     1441 tatcttccat tatgatgtag aagaattttt agatactaaa aaagtaaatg cggatgaaga
     1501 tttacgttta tctacaatat caactggttt aattgttcca tctaaattct tcgatttagc
     1561 taaagaaggt aaagactttt atatgtttgc acctcataca gttaaagaag aatacggtgt
     1621 tacattagac gatattgatt tagaaaaata ttatgatgat atggttgcaa atccaaatgt
     1681 tgagaaaaag aaaaagaatg cacgtgaaat gttgaattta attgcgcaaa cacaattaca
     1741 atcaggctat ccatatttaa tgtttaaaga taatgctaat agagttcatc cgaattcaaa
     1801 tattggacaa attaagatga gtaacttatg tactgaaatt ttccaattac aagaaacttc
     1861 aattattaac gactatggta ttgaagacga aattaaacgt gatatttctt gtaacttagg
     1921 ttcattaaac attgtaaatg taatggaaag cggaaaattc agagattcag ttcactctgg
     1981 tatggacgca ttaactgttg taagtgatgt agcaaatatt caaaatgctc caggagttag
     2041 aaaagctaat agtgaattac attcagttgg tcttggtgtg atgaatttac atggttactt
     2101 agcaaaaaat aaaattggtt atgagtcaga agaagcaaaa gattttgcaa atatcttctt
     2161 tatgatgatg aatttctact caatcgaacg ttcaatggaa atcgctaaag aacgtggtat
     2221 caaatatcaa gactttgaaa agtctgatta tgctaatggc aaatatttcg agttctatac
     2281 aactcaagaa tttgaacctc aattcgaaaa agtacgtgaa ttatttgacg gtatggctat
     2341 tcctacttct gaggattgga agaaactaca acaagatgtt gaacaatatg gtttatatca
     2401 tgcatataga ttagctattg ctccaacaca aagtatttct tatgttcaaa atgctacaag
     2461 ttctgtaatg ccaatagtag atcaaattga acgtcgtact tatggtaatg cggaaacatt
     2521 ttaccctatg ccattcttat cgcctcaaac aatgtggtac tacaaatctg cattcaatac
     2581 tgatcagatg aaattaatcg atttaattgc gacaattcaa acgcatattg accaaggtat
     2641 ctcaacgata ctttatgtta attctgaaat ttctacacgt gagttagcaa gattatatgt
     2701 atatgcgcac tataaaggat taaaatcact ttactatact agaaataaat tattaagtgt
     2761 agaagaatgt acaagttgtt ctatctaata agtaaacgta aaaaatgaca aacaactaat
     2821 catctcgttt aaaataacag atgattagtc tgccatgtct atatttgtca attattgaga
     2881 gtaacattac aggaggaaat tatattcatg atagctgtta attggaacac acaagaagat
     2941 atgacgaata tgttttggag acaaaatata tctcaaatgt gggttgaaac agaatttaaa
     3001 gtatcaaaag acattgcaag ttggaagact ttgtcagaag ctgaacaaga cacatttaaa
     3061 aaagcattag ctggtttaac aggcttagat acacatcaag cagatgatgg tatgccttta
     3121 gtcatgctac atacgactga cttaagaaaa aaagcagttt attcatttat ggcgatgatg
     3181 gagcaaatac acgcgaaaag ttattcgcat attttcacaa cgctattacc atctagtgaa
     3241 acaaactatt tattagatga atgggtttta gaagagccac atttaaaata taaatcagat
     3301 aaaattgttg ctaattacca caaactttgg ggtaaagaag cttccatata tgaccaatat
     3361 atggccagag ttacgagtgt atttttagaa acattcttat tcttctctgg tttctattat
     3421 ccgctatatt tagctggtca aggaaaaatg acgacatcag gcgaaatcat tcgtaaaatt
     3481 cttttagatg aatctattca tggtgtgttt actggtttag atgcgcagca tttacgaaat
     3541 gaactatctg aaagcgagaa gcaaaaagct gaccaagaaa tgtataaatt gctaaatgac
     3601 ttgtatttaa atgaagagtc atacacaaaa atgttatacg atgatcttgg aatcactgaa
     3661 gatgtgttaa actatgttaa atataatgga aataaagcac tttcaaattt aggttttgaa
     3721 ccttattttg aggaacgtga atttaaccca attattgaga atgccttaga tacaacaact
     3781 aaaaaccatg acttcttctc tgtaaaaggt gatggttatg tattagcatt aaatgtagaa
     3841 gcattacaag atgatgactt tgtatttgac aacaaataat aattaaaatt aaaaagacct
     3901 tcacatgaaa gggtaatagc gattcgtttc gtcttgactc ctacatgttg aaggtctttt
     3961 ttctataact taaaaacgta tgcacatttt gaaaaagtaa aaatggcatt taatgatgcc
     4021 aatgaggcta gaaagtatac aagatactgc attacaaaag ggtgtgctat tttactatat
     4081 acatctaaat tacacattta caaaaaatga tttcta
//
LOCUS       DAKSQE010000159         3961 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.161, whole genome shotgun sequence.
ACCESSION   DAKSQE010000159 DAKSQE010000000
VERSION     DAKSQE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.161"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..538)
                     /locus_tag="O0V33_002503"
     CDS             complement(<1..538)
                     /locus_tag="O0V33_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCX8977099.1"
                     /translation="MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNKILSLIGYSDTIVLQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFITSLPTIIVV"
     gene            complement(510..1484)
                     /locus_tag="O0V33_002504"
     CDS             complement(510..1484)
                     /locus_tag="O0V33_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HCX8977100.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMDKYI"
     gene            complement(1536..2159)
                     /locus_tag="O0V33_002505"
     CDS             complement(1536..2159)
                     /locus_tag="O0V33_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCX8977101.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESEKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            complement(2156..2485)
                     /locus_tag="O0V33_002506"
     CDS             complement(2156..2485)
                     /locus_tag="O0V33_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HCX8977102.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            complement(2485..3471)
                     /locus_tag="O0V33_002507"
     CDS             complement(2485..3471)
                     /locus_tag="O0V33_002507"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCX8977103.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            complement(3468..3671)
                     /locus_tag="O0V33_002508"
     CDS             complement(3468..3671)
                     /locus_tag="O0V33_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCX8977104.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            complement(3652..>3961)
                     /locus_tag="O0V33_002509"
     CDS             complement(3652..>3961)
                     /locus_tag="O0V33_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977105.1"
                     /translation="AQNIVKQTINKNYADDQTYYLSGMIMATINSKSEGMTEWERGLR
                     MFPKSGLLNFELAIANRSLNDDEKALKYVRKALNADPKNTDYINLEKELTKSNESKNK
                     "
BASE COUNT         1264 a          721 c          534 g         1442 t
ORIGIN      
        1 ttactacaat tatagtaggt aagctggtaa tgaaaaaaga caatgttttt tgaaaagaag
       61 atagttgaat attcatagaa gtatataatt gttgaaactg tggaattact gtgaggttta
      121 atataataat catagcaata aaaatagaaa ctagtattaa ggggtattgc agtgtcttta
      181 acaatcgttg ttctgacttt ctattcactt tcatataatt tacggtttct tctagaacgt
      241 ctatgatatt gccaaatctt tctgccaaat atacttgcag gacgatagta tcgctataac
      301 ctatcagtga taatatctta ttgcatggtg caccatttga aatttcactt agaatggtgg
      361 tacctaattg cttattttta tatgtcattt gaagatttaa aaattgaaaa ctttgataca
      421 gagtgaaacc atatttcaac aaattacata aatttgaaag taaatcgatt tgttgggcct
      481 tacttaattg tcgcttctta gaatgtagtt taaatgtatt tatccattgt agtttcacaa
      541 atgacacctg cttttgtcat atcatcaagt ttatcttcta agttcttaaa tgatgatggt
      601 aatgaatgat tatgggaaaa gaaatatcgg agttgttgct gagatagaat ttcacatact
      661 aattgtcgct gttgcttaat agtagttaca agtcgttggt ttataattaa gttagttgcc
      721 tgtatcaatt cttgtacaga aatgcccatt tctaatagcc ttaaaatagc acctttacaa
      781 tcagttgcat gcaatgtagt cagaacaagg tgaccactta aactagcctg tataacacac
      841 ttggcaacat ctttatctct gatttcacct attaaaataa catcaggatc acatcttaaa
      901 atagctttaa acgaatttac atagttaatg ccagctttat cattcacatt aatttgcacg
      961 ataccaggaa tttgcatctc tacaggatcc tctatagaaa ttacatttaa attcaaggct
     1021 ttattcgcgt atgagaccat ttgatacatt aatgtactct ttcctgaacc agttggccca
     1081 ctaaacaata gtaatccttg tttcttattc atgaggtgtt taaaatcatt gaatttataa
     1141 gttgatttct gttgttgaaa aaattgaggt acaattctga taacacaact ttcttggcca
     1201 agtgacaatg gtaaagttga tattctcaaa aaatatattt tattgaaatg gtaactatat
     1261 cgaccgctct gtgcgacttg ctgtgtagaa acatcaagcc cagcttgaaa cttcatataa
     1321 actaataact tttgataaat gctattccca atttgttcat actgctccaa gttatcatta
     1381 attctaaatt taatacttac ttcattttta actggaataa aatgtacatc actcgctttc
     1441 atttctatcg ctttattaat tatttcttga aatagaatct tcaaaaaaac acctcctaca
     1501 tataatcacg taggaggtgt ttttattact tcaatttaac cgtgtaaaaa tggatttaat
     1561 tgttcatcat caaccgtcgt atatggacca tgtccaggga ataaaggtaa atcgccttct
     1621 aattcaaata ttttatcttg aatagaatca actagcgttt catagtcacc tttatataaa
     1681 tctgtacgtc cgattccatt attaaataat gtatctccaa caactgcgaa ttcatcaaac
     1741 acatatgtta aacttcctgg tgaatgtcca ggtgtgtgta acacattaaa cttaaatcct
     1801 tctatttctg tgctaccttc gtttaacttt tcaggagtta ccttacttgt aataattggt
     1861 aatccatatt gcttaaattt atctgctcca tttttaacgg gatcttttag aaaatcaaac
     1921 tctgcttcat gcatataaac cgggacatcg aatcgatcaa ctatatcatc gactgctccg
     1981 atatgatcaa agtgtgcatg tgttaataaa atagctttta atggtttatt tatttggttt
     2041 aattttttaa taattttttc actttcacct gaagggtcaa tcagaataac agctttgtca
     2101 ttttcgatga aatacgtatt agtatcaact aagcctaaag ttaagcttga aatcctcata
     2161 ggttttcacc actattttct aaatgttttt gtactgatgt tagtttatca ttcatttttc
     2221 tagatttgtc tcgtcggtca tcaatacgga tatttgtaca aactctactt aaacctttat
     2281 caaaaggtaa ttcatgtatc acttgcacaa cttctaatac atcgcttaat tcaccttcaa
     2341 ttagagtatt cattggtgtt aattgaaaat caattttacc catcgcttta tattcttgaa
     2401 gttttttctg aatatctgct atatatttac taacactcgg accttccgtt ccaactggaa
     2461 taacaaccac atcaacaata gccattattt taccccctct ttatctaata cataggtctt
     2521 gattaatcct gctgctcctg taatacctgc atcattacct aatttcgctt gtacaatttc
     2581 agtttcaaat tgagcaggtg caaatgttaa attatgatat tctgttttaa tattttcaat
     2641 taaaataggt cctgcagtag acattcctcc acctagaacg atatatttcg gattacttgt
     2701 aacactaata atactacata aatatccaat atagtttgca accttttcag ttatgaaaat
     2761 acagaattgg tcaccagctt tagccgcatc aaaaacagct tttgctgtaa ccttattttc
     2821 tttaatcaat tctaatatag aagatctaaa cgtcaacttc ggatagtaga agttgactaa
     2881 gttaacaacg cctgtcgctg aagcaactgt ttcaatacat ccagaacgac cacaattaca
     2941 tttaaatcgt tgatcgaagt ctgctctaaa atgacctatt tctgcgccag agccattatg
     3001 accatgtacg atttcaccat ttgaaataat tcctccacct agtcctgtac caagtgtgat
     3061 ggcaacaaca tcatcggcac cttcaccagc acctttgtgt ttctccccta aagcagctat
     3121 gttagcatca ttatctacat acactggaca atcaacgaat tgttcaaaaa tctcacgtac
     3181 attaactttt tctggccaat ataagtttac tgctccattt actgtacctt tttcaaagtc
     3241 aacaggacct ggaacaccaa tacctacgcc aagtacattt gaaaaattat aattattttc
     3301 atttactttt tcaacaaacg aatcataaat tcctttcaaa agtgtataac ctgtactatc
     3361 agatgtatca gtgtgaatag accatttatg taattgttct aattcaggtg tgaaaatacc
     3421 taatttacaa gtcgtcccgc ctacatcagc tgctaaaata atttttgtca tttctgttca
     3481 ttccttctct gattaattat caacgtacat ttcaaatatt cttccttaga taacagttca
     3541 tattgataca atgatgaaat ctcctgttga atcatttcgt acatatcttc tggattttta
     3601 aaatatatta gaaatccgta acttttcaat aactgttgta catcataaaa gttatttatt
     3661 tttcgactca tttgatttag tcaactcttt ttctaagtta atataatctg tatttttagg
     3721 gtctgcattt aatgctttac gcacatattt taatgctttt tcatcatcat ttaatgaacg
     3781 atttgctatc gctaactcaa aatttaataa acctgattta ggaaacattc taagtcctcg
     3841 ctcccattct gtcatacctt cagacttaga attgatagta gccataatca taccacttaa
     3901 ataatatgtt tgatcatcgg cataattttt atttattgtc tgcttcacaa tattttgagc
     3961 a
//
LOCUS       DAKSQE010000160         3909 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.162, whole genome shotgun sequence.
ACCESSION   DAKSQE010000160 DAKSQE010000000
VERSION     DAKSQE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.162"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            133..504
                     /locus_tag="O0V33_002510"
     CDS             133..504
                     /locus_tag="O0V33_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCX8977106.1"
                     /translation="MLKKWLGMALITPMLTFIIWVFNSHTIITYLNILFYVSLIIFIS
                     TFLILLVQEGIFDATSYGFRRLKYQMSSSKKKKSISDDPFFNPQEVKKEHYFVSTWII
                     PLLLINILYFIMTIVLSLILV"
     gene            complement(559..1803)
                     /gene="fabF"
                     /locus_tag="O0V33_002511"
     CDS             complement(559..1803)
                     /gene="fabF"
                     /locus_tag="O0V33_002511"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCX8977107.1"
                     /translation="MSQNIRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINENTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVSS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(1815..2756)
                     /locus_tag="O0V33_002512"
     CDS             complement(1815..2756)
                     /locus_tag="O0V33_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCX8977108.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASVKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSEGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKLKDDDTIVLVGFGGGLTWGAMTIKWGK"
     gene            3051..3236
                     /locus_tag="O0V33_002513"
     CDS             3051..3236
                     /locus_tag="O0V33_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCX8977109.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
     gene            complement(3493..>3909)
                     /locus_tag="O0V33_002514"
     CDS             complement(3493..>3909)
                     /locus_tag="O0V33_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCX8977110.1"
                     /translation="ITVTLALGMIATTGATVASNEVSAAEKDKLPATQKAKEMQNVPY
                     TIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKSAVYTIT
                     WKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
BASE COUNT         1205 a          736 c          579 g         1389 t
ORIGIN      
        1 tttgctttaa actaatgaag aatgattgat tttagaattt caataaagct gaagaaatta
       61 aaaaatcatt cctcaatgtc tcattttaat gtaaactatg cgtaagagtt ttctataagt
      121 gggagtgatc aaatgttgaa aaaatggctt ggaatggcac taataacccc aatgctaacg
      181 tttattatat gggtttttaa tagccatact atcattacat atttaaatat attgttttat
      241 gtttcattaa ttatttttat tagcactttt ttgattttac ttgtacaaga aggcattttt
      301 gatgcgacaa gttatggttt tagaagatta aagtatcaaa tgtcatcttc gaaaaagaag
      361 aagtctatct ctgatgatcc atttttcaac ccacaagagg taaaaaaaga gcattacttt
      421 gtttctacat ggataatacc cttattatta ataaatattt tatattttat tatgacgatt
      481 gtgctgtcgc tcatcttagt ataaagaaac agaaaaaatc ttcagtaact aaaacaacgt
      541 tactgaagat ttttataatt atgcttcaaa tttcttgaat actaatactg cgttatgtcc
      601 accgaatcct aagctattac tcattgcata agtaatatca aggtcttgcg cttcatttgg
      661 aacaatatcc aaatcacatt ctgggtctgg tgttaccgca tgaattgtag gtgcgacttt
      721 agagtcttta attgaaagcg ctgagaagat tgcttcaatt ccacctgttg caccaagtaa
      781 gtgaccagtc attgattttg ttgagctaac ttttaagtgt ttagctgctt caccaaatgt
      841 atttttaata gctttaactt catttaagtc accaacagga gtacttgtac catgggcatt
      901 taagtattgt acatctttag gttcaatacc agcatcatcc atagctgctt gcattgctct
      961 agaaccgcct tcaccttctg gagctggcgc tgtaatatga taagcatcac ctgtagtacc
     1021 atagccaact atctcagcat aaatatttgc acctcgagct tgtgctgatt ctaaagattc
     1081 gattactaaa ataccagcac cttcacccat aacaaaaccg tctctacctt cttggaatgg
     1141 acgacatgct gtttcaatgt catcatttgt agaaagcgct cgacttgcac tgaaacctgc
     1201 aattgccata tgagtaattg gagcttccgt accgccagta atcattgcat ctgcatcacc
     1261 gcgttgcaca attttaaatg cttcaccgat agagttagta cctgttgcac atgctgtaac
     1321 tgttgcacca tttggtcctt ttgcacctaa gtcaattgat acttgtccag ttgccatatc
     1381 aggaattaac attggtacga aaaatggact cacacgtctt gggcctttat ccattaattg
     1441 tttatgtgca atttcaaatg tttccattcc accgatacca gaaccaatcc atacaccgat
     1501 tcgatctgca gtattttcat tgatatctaa ttgcgcatct ttaacagcct ctctagctgc
     1561 tacaattgca tattgagtaa atctatccat acgacgcgct tcttttttgt cgatatgatc
     1621 ttcaatatta aagtttttaa gttctcctgc taagtgaacg ctataaggtt cagtatcgat
     1681 acgtgtaatt ttatcgatac catttacgcc ttttagagca ttctcccatg ttgttttgac
     1741 atcattaccg attggagaaa gggctcccat acctgtaata actactctta tattttgact
     1801 cattcgttat cctcctattt tccccatttt attgtcattg cgccccaagt taggccgcca
     1861 ccgaatccga caagaacaat tgtatcatca tctttgagtt taccattttt taattcttga
     1921 tcgatactta aaggtattga cgcagctgaa gtatttccat atttatttac agaaacactc
     1981 attttgtctt ttgaaatacc taagcgttct ctagctgatt ccataattct aatattagct
     2041 tgatgaggaa taaataaatc tatatcatct gatgttaaat tcgctttttc aactacacgt
     2101 gttgatgcat cacccataat tctaacagca aatttaaata cttctcgacc attcattttc
     2161 agtttaccag tatctttatc taaatataaa tgtttaccac ctgtgccatc agaacccatt
     2221 tcataactta taatacctct gccttctgaa acttcaccga tgataaccgc acctgcacca
     2281 tctccaaata gaactgcagt agaacggtca gttaaatctg ttattttaga taatttatct
     2341 gcaccgacaa ctaaaatatt atgataatct ccagattgaa catattgttt agctgtaatc
     2401 attgaataca taaatccaga acatgctgca agttgatcca tagaggcaac tttgcccgtc
     2461 cctaaacgtt cttgtaacat atttgcgaca gttggaaatg gcatatctcc agttgctgtg
     2521 gcaacaatta tcatatctat atcttcgggc tgaataccag cgtcagcgat tgcttttaca
     2581 cttgcttcat atgctaaatc tgaagtatct tgatcgtcat ctgcccaatg tctttcttta
     2641 attccagtca tcttagaaat ccattcatca gatgtatcta aaaattgctc aaaataggca
     2701 ttgtcaataa tcttttctgg tgcatatgca ccaaaacctt taatacccac gttcatagtt
     2761 gaacaccttc ttaataaaat ttttaatacc aggtattaat ttaacataga ttacttttta
     2821 aactcaagtc agaggtagga atattgacta gttaataaaa ttttaatgct ttattatcgt
     2881 caaatttcag tatgaaattg attttaatgc gcttaattta gaatagccat tcagataaaa
     2941 tcatttattt aaactgtata atatggtcaa aatatatgag atgttactat acaaatttct
     3001 aactttcact taaaataaaa tatatataat taaagtggag gtagaatgac atgaaacacc
     3061 tcgtaacatt cttttgggca ttattattaa tgcaaatggt taactttgta cttaatagcc
     3121 tagtaggtgg agacagctta aatgtagtga atccaattat catggcagta ttgttcacga
     3181 ttttcacagc aattttcgct gcagtaatta aaccgccgaa agattcatca caataactat
     3241 ttagtataaa atgaaatatg tctttcttat tattgaagct gcccagataa aggcggcttt
     3301 tatttttggc cattaatgtt ttgtatttgg aatattttaa aactcaatga cctctaaccc
     3361 atcactttta aatttagaaa aagccttaat acaaagatgt catttacgag taatctttgt
     3421 atcaaggcaa ttatattata atcatcactg taaaattaaa tgcttcatgt tgaatttgat
     3481 aattttaagt gtttatttag tttttacatt aatatcaatt tgcttaattg aatttgaatc
     3541 aaacaagttt gctgtataac tatctttctt taaatccact tcttttttac tgccatcttt
     3601 ccaagtaatc gtgtaaacgg cagattttgc atttcgaatc ttctcaggtg ttacaccacg
     3661 ttcatcatat aataaagctt taactttatt ccctaaatct aaatatgata attggctatc
     3721 ttttggtaaa tttaagtaag attgattgaa agccataatg ccatctactg caattgtata
     3781 tggaacattt tgcatttctt tagctttttg agttgccggt agtttgtcct tttctgctgc
     3841 agatacctca ttacttgcca cagtagcgcc agtcgttgcg atcatgccca gtgccaaagt
     3901 tacagttat
//
LOCUS       DAKSQE010000161         3887 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.163, whole genome shotgun sequence.
ACCESSION   DAKSQE010000161 DAKSQE010000000
VERSION     DAKSQE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.163"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            251..1192
                     /locus_tag="O0V33_002515"
     CDS             251..1192
                     /locus_tag="O0V33_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCX8977111.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSKEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     RDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
     gene            1313..1867
                     /locus_tag="O0V33_002516"
     CDS             1313..1867
                     /locus_tag="O0V33_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCX8977112.1"
                     /translation="MTTKQLVYTALMTAIIAILGLVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSTGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGLK"
     gene            1939..2709
                     /locus_tag="O0V33_002517"
     CDS             1939..2709
                     /locus_tag="O0V33_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCX8977113.1"
                     /translation="MSHISMSDINCDGTIFENNDKQLIYLTPIFPQRYTSNTWLYKKM
                     PTQEQWLKDLKRQHQLHTNQGSNHYAFSFPENEQLDDTWMAIFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAIVDESHIDAYLKVAYQFSLPFGRDYADAHEKMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTMQSLVGEYALSRNH
                     KPVILVADGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(2880..3677)
                     /gene="fdhD"
                     /locus_tag="O0V33_002518"
     CDS             complement(2880..3677)
                     /gene="fdhD"
                     /locus_tag="O0V33_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCX8977114.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAVAKTSMSKITLTPQQIINMMTRLQSASHIYQETGGLHNAA
                     LSDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
BASE COUNT         1333 a          552 c          702 g         1300 t
ORIGIN      
        1 tagattagta ttattacagc taaagtagta tacctgagaa aatagctcaa tgtatctctg
       61 cattaataag ttatatcata attattttag tgcatacttt atggaaggca tatcagggaa
      121 tgcctatcaa ttaaagaaga ggtttaaaag gattacaaca gaatgttatg attttgtaga
      181 aagatatatt acaacgtttt ataaaaacat aatattgtta atggaaaatg aaatgtaagg
      241 gggatttcga gtgactaaga aagtttattt taaccatgat ggtggtgtag atgatttagt
      301 atctctattt ttattattac aaatggaaaa cgttcaattg ataggggtca gtacaattgg
      361 tgctgattgt tatttagaac catctttgag cgcatcagta aaaattatta atcgtttttc
      421 aaaggaagat attcaagttg cgccatcata tgaacgagga aaaaatccat ttcctaaaga
      481 atggcgtatg catgcctttt ttatggacgc attgccaatt ttaaatgagc cagtcaaaca
      541 cgttgcttca aatgtgagag acaaagaagc ctttgaagac attattcaaa ctttaaagag
      601 acaatcagaa aaagtaacat tattatttac aggcccgctt acagatttag caaaagcact
      661 acaaaaagat tcatcaatcg ttcagtatat agaaaaatta gtttggatgg gtggtacctt
      721 tttaccaaaa ggaaatgttg aagaacctga gcatgatggt tctgcagaat ggaatgcata
      781 ttgggatcca gaagcggtta aaattgtttt tgatagcgat atagagattg atatggttgc
      841 tttagaaagt acgaatcaag taccgctaac gttagatgtt agacaaagat gggcgaatga
      901 acgccaatat acgggcattg attttttagg agtaagttat gcagcggtac caccattaac
      961 acactttata acaaattcta cttacttttt atgggatgtt ttaacgactg cttatattgg
     1021 taacaaggac ttagttcact caattgagaa aaaagtggat gtaataagtt atggaccaag
     1081 tcaaggtaag acatttgagt gtaaagatgg gcgcaaaatt aatgtcataa atcatgtaga
     1141 taacaacgca ttttttgatt atataactgc acttgctaaa aaagtaaatt aacagctgtg
     1201 tagaataatt aaggttttaa tttatataga acaacttatt gtaaactttt cgttccttaa
     1261 agtttacaat ggtgctataa taatggtcat gaaatacgaa aggaagtaaa aaatgacaac
     1321 aaaacagtta gtatacacag ctttaatgac agcgattatc gctattttag gattggtacc
     1381 ggtaattcca ctaccatttt cttcagtacc aattgtactt caaaacattg gtattttctt
     1441 agcaggtgtg attttaggac gtaaatatgg cacattaagt gttatcgtct ttttattatt
     1501 agtagttgct ggtttgccac tgttatcagg tggtcgcggt ggcattggtg tattcgcagg
     1561 tccttcaaca gggtttttac tattatatcc agttgtagca ttcatgattg gtgcgattcg
     1621 tgatagattc atcaatgaaa ttaatttctg gattttattc gttggtattt tagtttttgg
     1681 tgttatagca ttagatgtta ttggtacatt gattatgggc atgattatta acataccatt
     1741 tacgaaagct atttcaattt cattagctta tttgcctggt gatatattaa aagcaattgt
     1801 agcaagtttg attggtacag ctttacttaa tcactcgcag tttcgtcaaa ttatgggatt
     1861 aaagtaatca tatttaagat aaaaaagtaa ttgattaagt tgcttttcaa ttgttgaaag
     1921 tgaaaggagt aggtgccaat gtctcatata agtatgtcag atattaattg cgacggtaca
     1981 atatttgaaa ataacgacaa gcagttgatt tatttaacgc ctatttttcc acaacgatac
     2041 acaagcaaca catggttata taaaaagatg cctacccaag agcaatggtt gaaagactta
     2101 aagcgtcaac atcaattaca tacaaatcaa ggttcaaatc attatgcatt tagttttccg
     2161 gaaaatgaac aacttgatga cacttggatg gctattttta aagatatgaa ttttgaacta
     2221 ggtattatgg aattgtatgc catagaaagt gatgcgcttg ctaatttgcc gcgtaactct
     2281 gacgttgaaa ttgctatcgt tgacgagtcg catatagatg cctatttaaa agttgcatat
     2341 cagtttagtt tgccatttgg aagagactat gcagatgcac atgaaaaaat ggtaagggaa
     2401 cattatcaaa aagatgtgat taaacgctta gtagcttatt taaataatga acctattggc
     2461 gttgtagatg tcattgaaag tgaaaattac attgaattag atggatttgg tgtactagaa
     2521 caatttcgac accaaggaat cggttctacc atgcagtcat tagtaggtga atatgcttta
     2581 tctagaaatc ataaaccggt tatattagtt gcagatggcg aagatacagc aaaagatatg
     2641 tatgcaaagc aaggttatgt ttaccaatca ttttgttatc aaatattaaa agaagatatt
     2701 ggaaattaac gattgatgat aggtttgtat tagaataaaa aagcagtaaa ctggtttcga
     2761 gtagaaatca acttactgct ttttaaattg ttttgagcta cttatactta taaaaatagt
     2821 gcttttatat tgttgattca tgtagaatac gttcattatg acatactata atgaatatgt
     2881 tattgttcag aatcaatgat acgttctgga tgactgtata tattaaagcc accatttcga
     2941 ataaatccaa ctgccgtaat atttaggtca ttagctaagg ttacagcaag cgttgtcgga
     3001 gcagatttag ataaaatgac gccaacacca attttcgcgg ctttaattaa aatttctgat
     3061 gaaatacgtc cactgaaaat taatacttta tctcggacag taatatgtcg ctgaatacaa
     3121 aatccatata atttatctag agcgttatgt ctgccaatgt cttgtcgatg tacaaaaaat
     3181 gtcaaaccat cgcttagagc agcattatgc aggccacctg tttcttggta aatatgactg
     3241 gcactttgta aacgcgtcat catattaata atttgttgtg gtgttaaagt gattttagac
     3301 atagatgttt tagcaacagc agcatcattt tgaaaataaa actcacgact ctttccacaa
     3361 caagatgcaa tcattcgttt tgtggaatat tgaaagcgat cgcctaaatc tttattcagt
     3421 tcaacatggg caaatccttt actatcatca atcagtacag attttaattc atctcgcttt
     3481 aaaatggcac cttccgaagc cagaaatcca atgactaact cctcaaggtt tgttggactg
     3541 catataacag tcgcaaattc ttcaccattc accataattg taagtggaaa ttctgtcaca
     3601 tattgatctg ttgtattgaa taattttcca tcttcatatc taacaattgg ttgacctaaa
     3661 gatacatctt tgttcattat ctaacccctt taattagctt gaactttatt ttaaatcaat
     3721 atgcttaata ttttaacata tttgcttaaa actgaaattt gattaactaa ataaaatagc
     3781 aggcaattat ttagaatttg attggattta gaaaaaggca cactagcagc tcaattaata
     3841 aaaaataatt tttagaaagt gtgacaatta tttgaatcat tatttaa
//
LOCUS       DAKSQE010000162         3864 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.164, whole genome shotgun sequence.
ACCESSION   DAKSQE010000162 DAKSQE010000000
VERSION     DAKSQE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.164"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..209
                     /locus_tag="O0V33_002519"
     CDS             <1..209
                     /locus_tag="O0V33_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977115.1"
                     /translation="FFIPNFQDIKYQPLLGYAAFLAIMSLLISFLLWYFTRNWRKERK
                     ARKLEKQIEKYGYEGAELRRKDKK"
     gene            157..342
                     /locus_tag="O0V33_002520"
     CDS             157..342
                     /locus_tag="O0V33_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977116.1"
                     /translation="MKNTAMRALNFAVKIRNNIRQLYAQNHQLLSYTAVTIISKVVEY
                     SAIVVFVLTFLYFTWLT"
     gene            513..1499
                     /locus_tag="O0V33_002521"
     CDS             513..1499
                     /locus_tag="O0V33_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8977117.1"
                     /translation="MFIIKSILYRLIQMIVVLFVISTLAFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKLSDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVLTLSLGMCAYIIRLVRSNLLILLKSNIVQASRLRGMNERY
                     ILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKNKTNTPLISESSDRHA"
     gene            1492..2322
                     /locus_tag="O0V33_002522"
     CDS             1492..2322
                     /locus_tag="O0V33_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCX8977118.1"
                     /translation="MHKIFSKNNLIFFVFGAFIFVMIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITVIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPIRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYNESH
                     "
     gene            2309..3082
                     /locus_tag="O0V33_002523"
     CDS             2309..3082
                     /locus_tag="O0V33_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8977119.1"
                     /translation="MSLIDIQNLTIKNTSEKSLIKGIDLKIYNQQINALIGESGAGKS
                     LIAKALLEYLPFDLTCTYDSYQFDGENVSRLSQYYGHTIGYIYQNYAESFNDHTKLGK
                     QLTAIYRKHYKSCKEEALSKIDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LKPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYIYVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            3075..3776
                     /locus_tag="O0V33_002524"
     CDS             3075..3776
                     /locus_tag="O0V33_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8977120.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPVKGEIYLSDNAVLPIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLIQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFTIEELFNV
                     DRHPYTKELVQAFSY"
BASE COUNT         1304 a          545 c          656 g         1359 t
ORIGIN      
        1 cattttttat tccaaatttt caagatataa aatatcaacc attattagga tacgcggcgt
       61 ttttagcgat catgagttta ttaattagtt ttctattatg gtattttaca agaaattggc
      121 gtaaagaaag aaaagcgcgt aaattagaaa agcaaattga aaaatacggc tatgagggcg
      181 ctgaacttcg ccgtaaagat aagaaataat attcgacaac tatatgcaca aaatcatcaa
      241 cttttaagtt acactgcagt aacaattatt tcgaaagtgg tggaatacag tgctatagtt
      301 gtttttgttt tgactttttt atattttaca tggttgactt gattctaaat attatatttt
      361 ataatgattt tgttaaaaaa ttaaatatac ttcaatttct ttttggataa tactataatg
      421 aacgttagtt cttaatatgt aagttgcata tacatgcgcc attctcatgt aaatagaaag
      481 tgttagtaat ttacacaaag agggggacta ttatgttcat aataaagtct attttgtata
      541 gactgataca aatgatagtt gttttatttg ttatttctac acttgcattc attttaatga
      601 aattatcacc aggcaatcct gttgataaaa tattacatct tgatgtagca caagtgtcga
      661 cggaacaaat aaatgcaacc aaagataaat tgggattaaa tgattcttta ttggtacagt
      721 ggtggcattg gatgaatcat ttattacatt ttaatttagg aaaaagtttt gaatcaaaag
      781 aaccggtaac acagatatta ttcaactatg cacccataac actactcata tctttttcaa
      841 cattagtagt gtcattatgc atatctatac cgctcggcat tatagcagcc aaacgatttc
      901 ataagttatc tgacaaagtg attagagtga tctcaactct atcaattagt ttaccagcgt
      961 tctttatcgg tattatttta ttatttattg tcacaaattt aatgaatata gatagcgtta
     1021 tactaagtca gtttatatta cctgtactca cgctatcttt aggtatgtgt gcatacatca
     1081 ttcgcttagt gcgttctaat ttattgatat tattgaaaag taatatcgta caagcatcaa
     1141 gattacgcgg tatgaatgaa cgctatattt taattcatga tttactaaaa ccaacaattt
     1201 tgccgattat cccattatta gggatttcac ttggtagtct aataggtggt actgtagtga
     1261 ttgaaaattt atttgatata cctggtattg gttatctatt aatggatagt attaaatctc
     1321 gagattatcc tgttattcaa ggatgcgtgt tatttattgg cttcttcgtt gttattatca
     1381 atacgattgc tgatttatta acgttattac ttgatccgaa gcagcgttta caattaggaa
     1441 atccgaaaaa caaaaccaat acaccattga tatcagaaag tagtgaccgt catgcataaa
     1501 atattttcaa agaataacct tatctttttt gtattcggcg catttatatt tgtgatgatt
     1561 gtactacaat tctttgtcag tagtgaaaat gcaaccaaag tcaatttatc acaaactttt
     1621 gaacctatta gttggttgca tttattagga actgatgatt atgggagaga tttatttacc
     1681 cgaattatta tcggtgcacg ttcaacattg tttgttactg ttttaacatt aatagctatc
     1741 gttgtcatag gtgttacact aggtctattt gccggatata aaaaggggtg gattgaacga
     1801 ttagtgttaa ggtttattga tgttggtcta agtattccag aatttatcat catgattgct
     1861 ttagcaagct ttttccaacc atcgttatgg aatttagtta tttcaattac agtaataaaa
     1921 tggatgaatt acacaaggtt gactagaagt atagttaata gcgaaatgaa taagccttat
     1981 ataaaaatgg cacaattatt tcatgtacct ataagaacaa tattaatacg tcatttaaca
     2041 cctaaaatta taccggctat tatcgttttg atggtcgttg atttcggtaa aatcattcta
     2101 tatataagtt cactatcatt tattgggtta ggtgcacaac cgccaacacc agagtggggc
     2161 gctatgttgc aacaaggtcg tgattttatt tcgtctcatc caattatgtt gattgcacct
     2221 gcttcagtca ttgctataac tattttaatt tttaatttaa ccggtgatgc gctaagagat
     2281 agattgctga aacaacgagg tgaatataat gagtctcatt gatatacaaa atttaacaat
     2341 aaagaatact agtgagaaat cacttattaa agggattgat ttgaaaattt ataatcaaca
     2401 gattaatgcc ctgattggag agagcggcgc tggaaaaagt ttgattgcta aagccttact
     2461 tgaatattta ccatttgatt taacctgcac gtatgattcg taccaatttg atggggaaaa
     2521 tgttagtaga ttgagtcaat attatggtca tacaattggc tatatttatc aaaattatgc
     2581 agaaagtttt aacgaccata ctaaattagg taaacagtta actgcgattt atcgtaagca
     2641 ttataaaagt tgtaaagaag aggctttgtc caaaattgac aaggctttgt cgtgggttaa
     2701 tttacaaagc aaagatatat taaataaata tagtttccaa ctttctgggg gccaacttga
     2761 acgcgtatac atagcaagcg ttctcatgtt gaaacctaaa ttaatcattg cagacgaacc
     2821 agttgcatca ttggatgctt tgaacggtaa tcaagtgatg gatttattac agcatattgt
     2881 attagaacat ggtcaaacat tatttattat cacacataac ttaagtcatg tattgaaata
     2941 ttgtcagtac atttatgttt taaaagaagg tcaaatcatt gaacgaggta atattaatca
     3001 tttcaagtat gagcatttgc atccgtatac tgaacgtcta attaaatata gaacacaatt
     3061 aaagagggat tactatgatt gagttaaaac atgtgacttt tggttataat aaaaagcaga
     3121 tggtgctaca agatatcaat attactatac ctgatggaga aaatgttggt attttaggcg
     3181 aaagtggctg tggtaaaagt acgctcgctt cattggttct tggcttattt aaacctgtta
     3241 aaggagaaat ttacttaagt gacaatgctg tgttaccgat tttccagcac cctttaacta
     3301 gctttaaccc tgattggacg attgagacct cattaaaaga agcgttatat tattacagag
     3361 gtctaactga taatactgct caggaccaat tattaataca acatttatct acttttgagt
     3421 tgaacgcgca attattgact aaattaccaa gcgaagtgag tggcggacaa ttacaaagat
     3481 ttaatgtcat gcgttcgtta ttagcacagc ctcgcgtttt aatatgtgat gagataactt
     3541 caaatttaga tgttatagct gaacaaaatg taataaatat attaaaagcg caaacgatta
     3601 cgaacttaaa tcattttatc gttatttctc atgatttatc cgtgttacaa cgcttagtca
     3661 atagaattat cgttcttaag gatggcatga tagtcgatga ttttacaata gaggaattat
     3721 ttaatgttga tagacaccct tatacaaaag aattagtgca agcattttca tattagttat
     3781 ttaagaatgc gataattcta gacttgttat aaaatataga taaatcaagt atattaatct
     3841 agacacatat cgattttatt ttct
//
LOCUS       DAKSQE010000163         3781 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.165, whole genome shotgun sequence.
ACCESSION   DAKSQE010000163 DAKSQE010000000
VERSION     DAKSQE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.165"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..1708
                     /gene="lanM"
                     /locus_tag="O0V33_002525"
     CDS             <1..1708
                     /gene="lanM"
                     /locus_tag="O0V33_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001798254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 2 lantipeptide synthetase LanM"
                     /protein_id="HCX8977121.1"
                     /translation="VIPQSKNVVRHQNEIIDYKDFIENIIEGFKCTCAIVMKKKDCII
                     KIIDDYKNQIIRVIPRSTDKYVQFLQYTYHPSALKSYLNLESILENLYALPFKERKII
                     KSEYYDLLNGDVPVFFSRIDSKDIIDSKGRFINNVYFRSNYSLVHDKINSLDNSICAN
                     QIAIVQSSINTLPDNDIDGFFIKDEKVNDKEILEIIEVTLESILGKGIIYKDLITWID
                     MLQDGSGNKKPKILNTNLYDGISGISLVLVYIKKIYPEKFDRYMELTLNNSINRPNVD
                     YSAISAYNGFASNIYPILKHMDGSFRGKRYKRVLTQFINSLESFEEKDINNYDWLSGI
                     GSLPSLINHIYNMLPDGHLLNILNKFADILIRQKTEVFAIDNLGLAHGLSGYLLSLKN
                     VNLNIEREDVQNLILEIEEKLIKSYDSNLSGWKFKRNKEMICTNYWCTGSIGIALSLS
                     HLVSKKNEEKINEIQKDLEEYYIDTNKLLRSDTLCHGNLGVIDYLIYRYNITGNEILL
                     EKAKKIFSNIIRKQKKYGHYSLNSYGEYPSTSLFTGKLGLCYIGLRLLNPKTIDSVSI
                     LE"
     gene            1720..2418
                     /locus_tag="O0V33_002526"
     CDS             1720..2418
                     /locus_tag="O0V33_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCX8977122.1"
                     /translation="MKKVICIVGSRNPSSKGLAFCKALLIRLKDFYHEIDYKILTPND
                     YFLEPIDGTNETFYKGIDKKDNIDDGSMIKHNLEDADFVIFCTPVYSHNVSGDIKILI
                     DRLSYWGHIFKLLGKSGLTVTVADSNGFIQVDEYIDKILTGFGVDVIENVILTRKDDV
                     QKFDFLSLIGDIVETFQPEYKIKVPAFTEAQFKSYKNSIRKQPHENFEYRYWDENKLF
                     YMETLQEYVDKYIN"
     gene            2439..>3781
                     /locus_tag="O0V33_002527"
     CDS             2439..>3781
                     /locus_tag="O0V33_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HCX8977123.1"
                     /translation="MKKIVYSLIILLFLSIISMSTLSKVQASEDELMIYILTKKNIQE
                     KEFKKEIEGYTNTNVIYEVPEIGLYQVRGSQRELESIIEHSKIIKNHTLNTKKNPDKT
                     QIDYQTDTNQNSIISEKKDLQWDISRLTSNGKSFITKSNIHNTTVAIIDSGVGNHPDL
                     KNAVNIRKNLVPKGGNNGKEKLETGDISFHTDFLGHGTMLAGQMAADGLSKGLYPGLK
                     INSYRVFGSESAESIWVIKGIIEAAKDDSDVINLSLIEYVSRGDTINKDGKIDYTNKI
                     KYEAYERAVNYAKSRGSFLVTAIGNQSLNLKDKEQIYNYWKKKNPSLVTNSQIYAVPA
                     SFQNVISVGSINHQNTISNFSNHNSADIYTYGGDNRLVEDIGTEKYFSNRMFEKEWIL
                     ALSPQGGYTYTFGTSLSAAKVSAIAAATIDKNNYKNRPDLTQRDLYNMTSGNKER"
BASE COUNT         1494 a          462 c          634 g         1191 t
ORIGIN      
        1 tgtgattcca cagtcaaaaa atgtagttag acatcaaaat gaaattattg attacaaaga
       61 tttcatagag aatattatcg aaggatttaa gtgtacatgt gctatagtaa tgaaaaagaa
      121 agattgtatt attaaaatta ttgatgatta caaaaatcaa ataataagag taattccacg
      181 tagtactgat aaatatgtac agtttttaca atacacttat cacccttctg cattgaagag
      241 ttatcttaat ttagaaagta ttttagagaa cctttatgcc ttaccattca aagaaagaaa
      301 aattattaaa agtgagtatt acgatttatt aaatggtgat gttccggtgt tcttctctag
      361 gatagattcg aaagatataa tagattcaaa aggacgtttt attaataacg tttattttag
      421 aagtaactat tccttagtac atgacaagat aaatagttta gacaactcta tatgtgctaa
      481 tcaaatagca atagtgcaat caagtattaa tactttacca gacaatgata ttgatggttt
      541 ctttattaag gatgaaaaag taaatgataa agagatacta gaaattatag aagtcactct
      601 tgagtcaatt ctaggtaaag gaattattta caaagatctt ataacatgga ttgacatgtt
      661 acaagatggt agtggaaata agaagcctaa aatattaaat actaacttat atgatggtat
      721 cagtggaatt tctctggtgc tagtatacat taaaaaaata tatcctgaga aattcgatag
      781 atatatggag cttacattaa acaattcgat taatcgccct aatgttgatt actcagctat
      841 atctgcatat aatggttttg catcaaatat atatccgata ctaaaacata tggacggctc
      901 ttttagaggg aaaagatata aaagagttct aacacaattt ataaactctt tagagagctt
      961 tgaggaaaaa gatataaata attatgattg gctttcaggt ataggaagtt taccttctct
     1021 catcaatcat atttataata tgttacctga cggccattta ttaaatatac taaataagtt
     1081 tgcagatata ttgattagac aaaaaacaga agtgttcgcc attgataacc taggacttgc
     1141 acatggctta tctggttatt tactttcgtt aaaaaatgtg aatttaaaca tcgaaagaga
     1201 agatgttcaa aatctcattt tagaaattga agaaaagcta attaaaagtt acgacagtaa
     1261 cttatccggt tggaaattta aaagaaataa agaaatgatt tgtactaatt attggtgtac
     1321 aggatctatt ggaatcgcac tatcattatc acacttagtt tctaaaaaga atgaagaaaa
     1381 gattaatgaa atccaaaagg acttggagga gtactatata gacactaata aactattgcg
     1441 atcagacaca ttgtgtcatg gtaatttagg agttatagat tatttaattt atcgttataa
     1501 tattactggt aatgaaattt tacttgaaaa agcaaaaaaa atattttcta atattataag
     1561 aaagcaaaaa aaatatggcc attattctct caattcatat ggggaatatc catctacgtc
     1621 tttatttacc ggaaaattag gtttgtgcta tataggttta cgtttactta accctaaaac
     1681 tatagactct gtatctatct tagaatagga gaaaataaaa tgaaaaaagt aatatgtatt
     1741 gttgggtcaa gaaatccttc gtcaaaagga cttgcatttt gtaaggcact tcttattcgt
     1801 cttaaagatt tttatcatga aatagactat aagatattaa caccaaatga ttattttttg
     1861 gaacctatag atggaactaa tgaaacattc tacaaaggaa tagacaaaaa agataacatt
     1921 gatgatggtt ccatgattaa acataattta gaggatgctg acttcgttat tttttgtact
     1981 cctgtatatt cacataatgt ttctggagat ataaaaatat taattgatag attgtcttac
     2041 tggggacata tatttaaatt gcttggaaaa tctggattaa ctgttacagt agctgatagt
     2101 aatggattta tacaagtaga tgaatatatc gataaaatcc ttacaggatt tggtgttgat
     2161 gtaattgaga acgttatatt aactagaaaa gatgatgttc agaagtttga ttttctatca
     2221 cttataggag atatagtgga aacttttcaa ccagaatata aaataaaagt tcccgctttt
     2281 acagaggccc aatttaaatc atataagaat agtattcgga aacagccaca cgaaaatttt
     2341 gagtatagat attgggatga aaacaaatta ttttatatgg aaaccctaca ggaatatgta
     2401 gataagtata taaactagga aggagtaatt tgttataaat gaaaaaaatt gtatattctt
     2461 taataatctt gttgtttcta tctattatta gtatgtcgac gctgtcaaaa gttcaagcat
     2521 cagaagatga attaatgatt tatattctaa ctaagaagaa tattcaagaa aaagaattta
     2581 aaaaggagat agaaggttat acaaatacaa atgttatata tgaagtgccg gaaataggcc
     2641 tataccaagt gagagggtct cagagagagc tagaaagtat aattgaacac agtaaaatca
     2701 ttaaaaatca tacattaaat acaaagaaaa atccagataa aactcaaata gattatcaaa
     2761 ctgataccaa tcaaaatagc attatttcag aaaaaaaaga tttacaatgg gatattagta
     2821 gattaacttc aaatggaaaa agttttataa ctaaatctaa tattcataat accacagtag
     2881 ctattattga ctcaggagta ggcaatcatc cagatttgaa aaatgcagta aatataagga
     2941 aaaatctagt tcctaaaggc ggaaataatg gcaaagaaaa gctagagact ggagatattt
     3001 catttcatac cgatttttta ggtcatggaa ctatgctagc aggtcaaatg gcggccgatg
     3061 ggttatctaa aggactctat cccggattaa agataaactc ttacagagta ttcggatcgg
     3121 aaagtgctga atcaatatgg gtaattaaag ggattataga ggcggcaaaa gatgattcag
     3181 atgttattaa ccttagttta atagaatatg tttcaagagg agatactata aacaaagatg
     3241 gcaaaataga ttatacaaat aaaattaaat atgaggctta tgaaagagcg gttaattatg
     3301 caaaatcgag aggttcattt ttagttacag ctataggaaa tcaatcttta aatcttaaag
     3361 ataaagaaca aatttataac tattggaaga agaaaaatcc tagtctagtt accaatagtc
     3421 aaatatatgc agtacctgct tcatttcaaa atgttataag tgttggttca attaatcatc
     3481 aaaatacaat atctaatttt tctaaccaca attctgctga tatatatact tatgggggag
     3541 ataatagatt agttgaggac ataggtactg aaaaatattt ttcaaataga atgtttgaaa
     3601 aagaatggat tctggctcta tcaccacaag gaggatatac gtatacattt ggaacatcgt
     3661 tatcggcagc taaagtctca gccatagcgg cagcaactat agacaagaat aattataaaa
     3721 ataggcctga tttaacccaa agagaccttt acaatatgac gagcgggaat aaagaaagat
     3781 t
//
LOCUS       DAKSQE010000164         3761 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.166, whole genome shotgun sequence.
ACCESSION   DAKSQE010000164 DAKSQE010000000
VERSION     DAKSQE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.166"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(96..1601)
                     /locus_tag="O0V33_002528"
     CDS             complement(96..1601)
                     /locus_tag="O0V33_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCX8977124.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVILAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAFSPLIIKAMKGIR"
     gene            complement(1941..2441)
                     /gene="queF"
                     /locus_tag="O0V33_002529"
     CDS             complement(1941..2441)
                     /gene="queF"
                     /locus_tag="O0V33_002529"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCX8977125.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEQMAEHRLMNHDLYP
                     EKIDNR"
     gene            complement(2461..3327)
                     /locus_tag="O0V33_002530"
     CDS             complement(2461..3327)
                     /locus_tag="O0V33_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCX8977126.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFIYKYHQPMFGKLSSQPLLLTRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQTQVLYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKVIKK"
BASE COUNT         1354 a          595 c          592 g         1220 t
ORIGIN      
        1 tttatactga tatcttgaat ttaattatta gaaaccaata aaaaaacagt aagccattta
       61 aatgacttac tgttttttga attaggccaa caatattaac gaataccttt cattgcttta
      121 ataattaaag gtgagaatgc taatacaatt gttgtaacaa taattgcaac aacacctaag
      181 aagataaagt aatttgtttg acctagtggt tctattaact taactaatgt accattgatt
      241 gcttgtgcag aagcgttagt taagtaccaa atactcatca tttgggcatt aaatgcttta
      301 ggtgctaact taacagcagc actattaccc gttggtgata agcaaagttc accgataaca
      361 cagataatgt acgataaaat aacccagtta actgagaagt ttgatgaacc tgatgcataa
      421 cctacaatac caattagtat gtatgacgca cctgctaaga acgtaccaat tgcaaatttc
      481 actggcaggc taggttgttt agttccaagc ttttgccata aaagtgaaat aattggagct
      541 aataataaaa taaataatgg gttaattgat tggaaaatcg cttcaccaaa gtttgttttc
      601 caaccaaata agtttaattt catatctgaa tgttcaattc cataaatgtt caatacatta
      661 gacccttgtt cttgaatagc ccagaacacc attccaagaa taaataatgg aataaatgct
      721 ttaacacgag aacgttcagt atcagtgaca tctttacttc taataattaa agtgaagtaa
      781 atgattggta atgcaatacc taatactaaa acagtattac taactaagtt aaatgataat
      841 gagttagtta atgcaccaat aacgataatt aatacaattg ctaagataac acttccgata
      901 ataagaccat actttttctt ttcagctggt gtcaatgggt tagtaggttt cataccaacg
      961 ctacctaagt ttttgcggtt gaaaagtaca taccatacta aacctaatgc cataccaaca
     1021 gctgcaatca agaatccacc gtggaagttt ttaacattaa caaagtgttg caaaataata
     1081 ggtgataata atgcacccat attaactgac atatagaaaa taacaaaacc tgcatccata
     1141 cgtctatcat tttcaggata taaacggcca acgatatttg aaatgtttgg cttcattaaa
     1201 cctgaaccaa taatgatgaa gaacattgat gtgaataagc cgattaatgc aaatggtaag
     1261 cttaaacaaa tatgtccgat aataataaag actgcaccta ataaagtagc gcctctagta
     1321 cctgtaattc tgtcagcaat ccatccgcct ggtattgatg tcatatagat taatgaacca
     1381 taaactgaca taattgacat agctgttgtt ttatcaattc caaggccatt atctgttacg
     1441 gcaaagtaca tgtagaaaat gagtagggca cgcatgccat aataactaaa cctttcccag
     1501 aactctacaa agaagagtac gcctagccct cgaggatgcc cgaaaaatcc tgtttgaggt
     1561 atgtcttgaa tttgatttcc atgggagttt tgttgtgtca tttatacatc ccatcctttc
     1621 ttcccctaat acaatagttt aagtaaattt gattgtgcct tatcaactaa atatttcaca
     1681 aatcacaaaa aaataaaaat attgagaata ttctgttatt aagcgcgcac aattaattag
     1741 agataatata aaaatatact tattgacgtt aaagtgcaat aggtttctat aaaaatgttg
     1801 tttaattcgt gtactaatat tcttttggtg tactttggta taaaatgtta ttttaatgta
     1861 acctattatg tatgctaaat gaaaaacaca ccaataagtt aggaaaatca acttattggt
     1921 gtgtttatta aatgatacat ttaacgatta tctatttttt cgggatataa atcatgattc
     1981 attaaacgat gttcagccat ttgttcgtat ttagaatttg gtcgtccata gtttgtataa
     2041 gggtcaatag aaattccacc acgtggtgtg aacttgcccc aaacttcaat ataatgtgga
     2101 tccataagct ctatcaagtc attcataatt atattcatac aatcttcatg gaaatcaccg
     2161 tgatttctga aactaaataa gtaaagtttc aacgattttg attcaaccat tttaacattt
     2221 ggaatataag aaatatagat agttgcaaaa tctggttgcc cagtaatcgg acataatgat
     2281 gtgaattctg gacagttgaa ttttacgaaa tagtcacgac cttgatgctt attatcaaac
     2341 gattctaata catcaggacg atagtcaaaa ttgtacgtat tgtcttgatt tcctaataaa
     2401 gttatatctt gtaattcatc ttgttgacgg ccatgtgcca taaaaagcgc tcctttaaat
     2461 ttattttttt attactttgg cgtctcggcg tgctttttca aacatgtaat aacttgcacc
     2521 gataataacg acgtaaccta atgttgcata gaaatctgga gattctccaa atagaataaa
     2581 tccaagtatt gctgtgaaaa ttatagatgc atatgtgaaa atagaaatat ctttcgcagc
     2641 agcaaaacta tatgccaatg taacgccaat ttgacctaca gctgcagcta aaccagcacc
     2701 taatagataa agcacttgcg tttgactcat tggttcatag gtatatgcag tgaaaggtat
     2761 taaaacgatg acagaaaata aggagaagta aaatactata gtatatggtg cttctctagt
     2821 actaagtgcg cgtacacacg tatatgccga tgcagcaaaa atacctgaaa atagtccagc
     2881 tagtgatgga atcatagatg atgaaaattc aggtttcact attaaaagca tacctaaaat
     2941 agcaattatc attgctgtta tttgatattt ccttaccttt tcatgtaaga aaacaatact
     3001 taataaaatc gtccagaaag gattgagttt cattagtgaa tcagcatcgc taagtaccat
     3061 atggtcaatg gcgtaaatat ttaataatac acctatgagt ccaagtgttg atcgtgttaa
     3121 taataatggt tggcttgaga gtttaccaaa cattggttga tgatatttat atataaaaaa
     3181 taatggaata aacattgcta ctaagtttcg tgctaatgat ttttgaaaaa caggaagatc
     3241 acctgcaagt ctgaaaaaca ctgacataaa actgaaacca atagccgaaa ttaaaatggc
     3301 aatgatacct tttactttag gattcaattt tatcgcctct tttatataaa attaacgtat
     3361 ttatattagc ataaaacaac atgttgtgca taaatagttg aaatttacta taaaaagact
     3421 ataatagact ttatcgaaca agtgttctgt gtttatttgt cggaataata gggcattaca
     3481 cttttatgaa tgtttgtgtt attacataaa acaaatatca attcagtatc aagctaataa
     3541 gctttttctt gatttctgtt gatacaattg agattgacac agattttaaa aaatcaagtg
     3601 atatctactg aaaaaatttt tttaatttgt tcaagttttt ctaattaagt attggtgcct
     3661 agttggaacg ttttacgaac attcgattag aaaatgacac tttaaatcat agtgtgtctt
     3721 atgtataatg aaacacataa tatagtgttg gttaaacgaa a
//
LOCUS       DAKSQE010000165         3712 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.167, whole genome shotgun sequence.
ACCESSION   DAKSQE010000165 DAKSQE010000000
VERSION     DAKSQE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.167"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(349..773)
                     /locus_tag="O0V33_002531"
                     /pseudo
     CDS             complement(349..773)
                     /locus_tag="O0V33_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(813..1040)
                     /locus_tag="O0V33_002532"
                     /pseudo
     CDS             complement(813..1040)
                     /locus_tag="O0V33_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095317.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1031..2094)
                     /gene="istA"
                     /locus_tag="O0V33_002533"
                     /pseudo
     CDS             complement(1031..2094)
                     /gene="istA"
                     /locus_tag="O0V33_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215400.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(2214..2492)
                     /locus_tag="O0V33_002534"
                     /pseudo
     CDS             complement(2214..2492)
                     /locus_tag="O0V33_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2508..2645)
                     /locus_tag="O0V33_002535"
     CDS             complement(2508..2645)
                     /locus_tag="O0V33_002535"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCX8977127.1"
                     /translation="MKFETLEQLETELYDYVNWYNNFRPHSSSQYLMPVAFKNLHMKS
                     V"
     gene            complement(2658..2978)
                     /locus_tag="O0V33_002536"
     CDS             complement(2658..2978)
                     /locus_tag="O0V33_002536"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCX8977128.1"
                     /translation="METLVSDLTYVKVAGTWHYICLFIDLFNREIVGYSAGKNRDANL
                     VSKAISRINHNLEQIKLFHTYRGKEFDNHLIDEVLETFIIKRSLSFEGCPYDNAITEA
                     TMKD"
     gene            complement(3335..3445)
                     /locus_tag="O0V33_002537"
     CDS             complement(3335..3445)
                     /locus_tag="O0V33_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCX8977129.1"
                     /translation="MVRLYKNGKPRNEIIREYDLTPSALGKWIKQHQNTG"
BASE COUNT         1164 a          577 c          497 g         1474 t
ORIGIN      
        1 tattctatga agataagcat aatattattt atatgaataa aaaaaatgtg aattttaata
       61 actcggaatt agaatgtcaa ttgcaattag atgatggaga aaaaaatgta ttattgaaac
      121 aacttgtcaa tttttataaa tcgaccatta ttttaatgtt tattaccgaa ataacgttga
      181 tgttttactc cataatatca tctaactatc aaacagtgag tatggtttta attgctatag
      241 ttattttcat ttcatcttca ctactaatta caacgaatgt atatacaaat cacaactaat
      301 taataatggc tctatgtcaa attggactga tgagttcaat aatggaagtt aggcaactaa
      361 acattgctta acttcttttt tcctttttgg agcgtaaagt ttgaacataa taatattcga
      421 ttgtgcaaat gattgtaatt tccataacca aaagatgtac gtttaattaa ttttattttg
      481 ttatttatac cttccaaagg accatttgat aaattgtaat aatcaaatgt attttttata
      541 aagtatgcat gtttgtttag tgtcttaatt gctcgttgga caaatgaatg cgcttgaggg
      601 aatggcatat gttttaattc atgaacaaaa ttttcataat cattatgttt aagtttgaat
      661 cgtagttggt tgatatattg gtaagcgcca tttagcgagt catcttgatc taataaatac
      721 ttaacgatac ttttttgtgt cttccattcc ttaaataaag ctactttacg atatttgcca
      781 atagcggttt aaaaatgtct ttttttagcg gtttaagatt gtcgcatatt taaggatttt
      841 tcgtttttat aatcttttaa tcgatatgat tctccagtaa ttttaaatac ttttgaatgg
      901 cgaactaacc aatcaattat agctgctgat acaatcttat tactaaatga ctcttcccaa
      961 ctcgaaaatg gaatattagt cgtaattatt gttgatttga tttcatatct tagtgacatt
     1021 aactgataga ataaatcagc agttcatcag tagaattatt gaatatcact tgtacttctt
     1081 caccaatgta tttagtaggt accgagtatt taccttttct gaagttaatc atcgattctt
     1141 tagaaatgat tcttatacac tcattttcga tgtaagtatc tagtaacttg gcattaaaag
     1201 aatttaaatg ttctttttct tcataattga aaacgtcgat agatcgttgt tctgttgctt
     1261 gtgaaatttc taagtgattc aattcgtgac ataaatcgtt tactagccca ataagttcta
     1321 cagcatcata aaattcataa tcgtatggtc ttaaacgctg ttcaacaaat ttagctaatg
     1381 attcagcaga ccctttggtt tgaggacgat agggtctaca agcaataggt tcaaagttgg
     1441 catccttact aaattgataa aataaattat taaagacgac ttttttatat tgtgttctcg
     1501 gtctatcaac tacagttctc atattatcga accagatttc ttttggaacc ccttcggtgt
     1561 attcaaaagc gtcttttaaa cattcaaata aggtatcttg ttttctatcc caagttaatg
     1621 tgatatactt catttttgaa tagtgtagaa cgtaaagaaa gatattgaat tgataagttc
     1681 tgccaaactt atcatgcatt accatatctt ctttccagtc tacttgagca actataccgg
     1741 gattagtttc tacgcgtata attgcttttt tagtctcatt ttgttttttc agtattctcg
     1801 tagaatagta tatttacctt cataactctt tttctctata tatttaaaaa tggccatagc
     1861 tgtacatcct aattcaattt ttttattaat aatttcttta aatggatcta gttttgatgc
     1921 tttcttattt tgttgtcttt tcttcaatcg ttctaattca ttttcttttc ctgcttcata
     1981 atatttttta actgttcttg gatcgcaatt atattgtcga ccaagctcag cataatttgg
     2041 tttaatacct ttcataatgt aaaatagcac tccttcataa atgtcatgtc tcaattaaat
     2101 aaccccaatc gaagattgag ttaagacatg attatataat ttaaaaaaga aaaaagtagg
     2161 aaaaacagca ttttcatttc gccattttcc tacattttat aatcgccatt tacatattca
     2221 aaggcctcta aatcatctcc gggttttaat agtaatttag catatctttt aaacttatta
     2281 tataaagatt gattcgtcgt tcttaattca ttcataacag tgactcgaat gacatttaaa
     2341 gcgcgattta ataattgaaa aatatggaat cgatcgataa ctattttagc gttcggaaac
     2401 atctctttaa tcaatgtcat atagccttag tgcatatcaa tggaaacagt ctttacttgc
     2461 tttctatctt ttagtgaaaa acgataaaag tggtaagtca acatttttca gacacttttc
     2521 atgtgtagat ttttaaatgc tactggcatc aaatactgag atgaagaatg tggtctaaaa
     2581 ttgttgtacc aatttacata atcatataat tctgtttcta actgttctaa tgtttcaaat
     2641 ttcatttgtt ttacaaatta gtctttcatc gttgcttccg taattgcgtt atcataagga
     2701 caaccttcga aacttaatga acgcttgatt ataaaggttt ctaggacttc atctatcaaa
     2761 tgattatcaa attctttgcc tctataggtg tgaaatagtt tgatttgttc aagattatga
     2821 tttattctgc tgattgcttt tgatactaaa tttgcgtccc tatttttacc tgcactgtaa
     2881 ccaacaattt ctctattaaa tagatctata aataaacata tgtaatgcca tgttcctgcg
     2941 acttttacat atgttaaatc acttactaat gtctccattt gttgctctct attaaaaacg
     3001 cgattcaagt gatttttaat tcgtcttgta ccaaagactt ttctattaga attaaaaata
     3061 tttatgacgg catgttctac gtttgaatca tctttagtga ttttattatt ttttcttttt
     3121 atagaatcat aatagctact tcttggtatt tttaggactt tacacattgc cggaactgaa
     3181 tattgatgtg cattcttttg aatgacttct attttcgtcc tatgatcagc gctgcttgct
     3241 ttaaaatatc gttctccatt tttatatgtt gaacttcatt gcctaattta atcagctctt
     3301 tttctttatc tgttaagcta tcttgatgat tgaatcaacc tgtgttttga tgttgcttta
     3361 tccatttccc taacgcggaa ggtgttaaat catactcgcg tataatttca ttcctaggct
     3421 taccattttt atataatcta accatttata acttaaaatt tgaactaaat gatcttcttt
     3481 ctctagtcat aataaaatcg cctactttct taaattaaca aaatctactc tcatagaatg
     3541 tgtccaatta agtgtagacg attcattgtt cacctcaata aaattatgtc taggttttta
     3601 aaaaatgtac attattaaac tatcatttcc attctaacta ctatagcaac tgatgtctat
     3661 gattataaat cttttaatat tggtttaata taaacattcc atttctagaa ac
//
LOCUS       DAKSQE010000166         3688 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.168, whole genome shotgun sequence.
ACCESSION   DAKSQE010000166 DAKSQE010000000
VERSION     DAKSQE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.168"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..240
                     /locus_tag="O0V33_002538"
     CDS             <1..240
                     /locus_tag="O0V33_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977130.1"
                     /translation="AINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSF
                     TIAMITKNWLSEILFFINIVLVLYYIYPIFYSFIG"
     gene            269..457
                     /locus_tag="O0V33_002539"
     CDS             269..457
                     /locus_tag="O0V33_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977131.1"
                     /translation="MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVI
                     LYIFGFAILIYTFIFGVL"
     gene            645..1214
                     /locus_tag="O0V33_002540"
     CDS             645..1214
                     /locus_tag="O0V33_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977132.1"
                     /translation="MNYIKRTLILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPG
                     DIIITKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKV
                     YRCSYPGAGKKAADWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSV
                     KSYGLHIISPYAIKDNFIDPYKLRLVKAY"
     gene            1302..1586
                     /locus_tag="O0V33_002541"
     CDS             1302..1586
                     /locus_tag="O0V33_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977133.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNNGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEVLDDNIYNISILNKTFIEFEQNFG"
     gene            1648..1866
                     /locus_tag="O0V33_002542"
     CDS             1648..1866
                     /locus_tag="O0V33_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977134.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEIDLDN
                     GNVSNIDHSLLVSVDPKALEISIFDAVN"
     gene            complement(2363..2563)
                     /locus_tag="O0V33_002543"
     CDS             complement(2363..2563)
                     /locus_tag="O0V33_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCX8977135.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
     gene            complement(2920..3606)
                     /locus_tag="O0V33_002544"
     CDS             complement(2920..3606)
                     /locus_tag="O0V33_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCX8977136.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKEQYAKRFFFFATSCL
                     VLTLVVASSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
BASE COUNT         1249 a          583 c          488 g         1368 t
ORIGIN      
        1 gctatcaata tagttattac taccactttt gttatcttta atattttaat cacatataat
       61 aaagatttag atgatttatg ttggctcctg cctggtatta tcatttgtgg tgtgatactc
      121 atagtatcct ttaccattgc aatgataact aaaaactggt taagtgaaat attatttttt
      181 ataaatatcg tactcgttct ctattacatt tatcctattt tttatagttt tataggttaa
      241 taattacggt atataaggag ggtttcacat gtctttacat tttgcaattc tgttttggct
      301 agcattaatt ttcttagttg ccgctacgtt tatactcgta ttaatgaaaa aaactggcaa
      361 agaatctaaa aaagagtcct atttaagttt cactgtcatt ctctatattt tcggattcgc
      421 tattttaata tacacattta tatttggtgt gctataaatg aaattcattt tatatccagt
      481 ctgaaacgtc tcaaagatgt tttagactgt tttttattct taatagacat ttcacactct
      541 catactttaa tatttaagat atgttttagt cttgcatgct tattaaagat taaaattata
      601 attcaaggct atttacatat atttcttcaa caaggagctt ttaagtgaac tatattaaac
      661 gaacgttaat tttacttata ttattcgttg tcgtatcccc tataaactca ccaaaaacaa
      721 ttgcagataa taagtattca gaaattcaag atgacaaatt ccaattacaa cctggtgata
      781 ttattattac aaaaggccct gtcatgtggg gattttttgg tcattgtagt atcgcgattg
      841 atgataaaac gattttgcaa attgaggggc ctggcgacaa accaactaca caatccttcg
      901 aatcttttaa atataattat gcaagtggca aaaatgattg gatgaaagtt tatcgttgca
      961 gctatcctgg tgcaggtaaa aaagcggctg actgggttaa gaaaaactat gaaaatacaa
     1021 atcatcgtta tctagttaca ttaaatttga acagcaaaaa atttacgtat tgtactaaaa
     1081 ttatctatca agcttataaa tttggtgtaa gtgaaaaatc agttaaaagt tatggactac
     1141 atattatttc accctatgca attaaagata attttataga tccatataaa ctaagacttg
     1201 ttaaagctta ttaacacagt ttcatcaaat agttaaatta gtttgtgttt tgcaataaat
     1261 tgggtataga ttacaaatga tatttcagga ggcccaatat tttgaatgaa ctaactaaag
     1321 aacaaaagta tacaatagct aaattttaca aattatatat tgaacgttcg aataatggag
     1381 aaacagaaac agtcgctaac ttttttggcg atgctaaaga tgcacgtgaa aactattttt
     1441 gcgatcgtga ttatcaagac ttcttaacta actgccaaat tttaattcaa aacaaatact
     1501 taactggtga agttttggac gataacattt acaatatttc tattttaaac aagacattta
     1561 ttgaatttga acaaaatttt ggttgaacat gaatgtctca actataaact ttctaaccct
     1621 aatttaaagc aggagtgaaa aacatacatg agtgaatggc atattaataa tcaatctaca
     1681 aaaccttttt taatacaaca agcggaaaag caattgtcta ttgttaaacc gttgcctaat
     1741 ggtagcttcc aaatactcac agaaatagat ttggataatg gcaatgttag taacatcgat
     1801 cattctctac ttgtttcagt agaccccaaa gcattagaaa taagtatatt tgacgctgta
     1861 aattaaacac aatattttta tttaattatt ttaatgtttc gccttttaaa tgaaaacaag
     1921 attactgaat tattatgaga ttttaaaaac aataaatatt ctcaataatt aatcagtctt
     1981 aataaaaaat taacaaaaag ttgattagtt aaattattaa ttaagtagta agatattatg
     2041 cgatgtggta tcctattatt gttaaacatt ttgagtgttt aacattactt cttgttatcg
     2101 ccatatacag actttaattc tcgtcattat taaagttctg tattggcgct ttttttatat
     2161 tcaaaatcaa aagtcggaca gatgaaaagt aaaaaacttt tccatcagtt cgatttattt
     2221 tagaaccatt atatattgtt agatctccac ctaaatttat tggacaaaaa aataacagct
     2281 agtgctttta ccacaccaac tgctatcgat ttaaagcata aaaaaacagc cctataataa
     2341 ggactgtttg aataagttaa aattacattt taactacgtt tgcagcttgc tcgccacggt
     2401 cgccttcaac gatgtcgaat tcaacttttt ggccttcttc taatgatttg tatccatctt
     2461 cagcgattgc tgagaagtgt acgaatacgt cgctaccatc ttctctttcg atgaaaccaa
     2521 aacctttttc tgcattaaac catttaactg taccgttatt catattgaat acctccgtcg
     2581 tgcttttgca ctaaatattt tgtaacaaat tcataaatga aaaggagaat attctatata
     2641 aataactcac atttttcttc acgctcttta ttacttaacc ttcattatac acttttaaat
     2701 atagaagtaa agcattattt tattttctgt gataatacac attgaaatat atagtaatat
     2761 tccattttaa tactaaattt aaaattttta ttttctaata ttaaatacac ttgattttga
     2821 tacgatttac ttttttaatt ctgatttgat aataaatcat aacgtgatct aaaaattata
     2881 aaagtgccac tagcagcagt gacactttta caatgagcat tattgacctg aatcagcgtt
     2941 gtcttcgctc caaatattta atttctcttt tttcgcttgt gcttcacttt ttcttaaaag
     3001 ttgttcatgt gtattgttag gtttataaac ataagcaact ttagccaagc cttgacgaac
     3061 taaagcttcg tttaccattt ttccatcagc ataaatatac gctaagccac gtccatattt
     3121 atcagttctt tgacctttgt caaactcgac ttcaattttc tttgcatttt ctaccatctt
     3181 tttcgtaaat gcacttgctt caggaccata tttctctaca ccttttttag gatgctttgt
     3241 ttcaggtgta tcaaccaata atagtctgaa tgtcattggt tgacctttgt acattaattt
     3301 aacagtatca ccatcaatcg ctttaattaa tgtcgcaggt tctttatgta attttttagt
     3361 tgaagttgca ctatatactg ttggatcttc agaaccactt ctatttacgc cattatctgt
     3421 ttgtgatgca tttgctgagc tacttagact tgaagctaca actaaagtta acactaagca
     3481 actagtagcg aaaaagaaaa acctctttgc gtattgttct ttcgaaacat tactgatagc
     3541 catccctata agtaatattg aaacaattgc catacatatg ccagcactta ataagtattc
     3601 tgtcataact aacacctctt tctttttagt taattttaat attaaacgtt aattaattat
     3661 aattctacat ttttacaatt aattaata
//
LOCUS       DAKSQE010000167         3661 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.169, whole genome shotgun sequence.
ACCESSION   DAKSQE010000167 DAKSQE010000000
VERSION     DAKSQE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.169"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..601
                     /locus_tag="O0V33_002545"
     CDS             <1..601
                     /locus_tag="O0V33_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCX8977137.1"
                     /translation="KYVAKIDSETSKLCHSLNGKIFKVKDMIPGVNAPPMHPWCRSTT
                     VPHVGNWRDKFFKEREGKYQVEVKEAKLQEKAKNQMKEMIESGKIKIEINPEKQNRHS
                     LGHKLYLKNKIYALQNDERFPSYTILSIEELNDLLKKYSMTGKILVDKFGFNRKEIIN
                     FEKTIGKAYAGGKYINTAYGKIHYSKTGSHIVPFVSKEK"
     gene            603..800
                     /locus_tag="O0V33_002546"
     CDS             603..800
                     /locus_tag="O0V33_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977138.1"
                     /translation="MLIKDVYRKNVIVTLKNDEKLKGFVIDFENPLESDSGNYCMDLE
                     TDLGFYSIDESDIKDIQIITE"
     gene            865..993
                     /locus_tag="O0V33_002547"
     CDS             865..993
                     /locus_tag="O0V33_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977139.1"
                     /translation="MKVIFYCPKRADDILKASMEYQSTDYKRRYISWKKMKVILLM"
     gene            960..1544
                     /locus_tag="O0V33_002548"
     CDS             960..1544
                     /locus_tag="O0V33_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977140.1"
                     /translation="MEKNESNITDVTQNEEQLDNSDEQSQQNEKTFSQEEVSQLIKER
                     IARERKKSDERIKNAKENNDSNEVAYLLKGAKVTKVYGDQNSVSFVPGEKATELLFDS
                     KPNSIVMLHNHPGQSGFSLNDLAVFTINNSIKTMTIVTNKGRIKFISKTEHFKEKVMK
                     KMIANLLIEKSLDVISTKDIERLLKELYNNDNII"
     gene            1742..2056
                     /locus_tag="O0V33_002549"
                     /pseudo
     CDS             1742..2056
                     /locus_tag="O0V33_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282612.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            2213..2386
                     /locus_tag="O0V33_002550"
     CDS             2213..2386
                     /locus_tag="O0V33_002550"
                     /inference="COORDINATES: protein motif:HMM:NF026880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977141.1"
                     /translation="MSTGNLILSDERFDLKDIINFNQIIGKIHIGNVYIETIKEKVHY
                     SKTGAHIVPYIDK"
     gene            2397..2591
                     /locus_tag="O0V33_002551"
     CDS             2397..2591
                     /locus_tag="O0V33_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977142.1"
                     /translation="MRIEDAYRKDVIITLLNNEEYEGFVTDYENEFESETGNLVVDIQ
                     TDFAVYSFDETEIKSIRLLK"
     gene            2672..3335
                     /locus_tag="O0V33_002552"
                     /pseudo
     CDS             2672..3335
                     /locus_tag="O0V33_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463904.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            3348..3596
                     /locus_tag="O0V33_002553"
     CDS             3348..3596
                     /locus_tag="O0V33_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977143.1"
                     /translation="MLILKIKEINDKSVTYKYFPNNDENIKPGIIQMDIDSLEVINAE
                     KSSLEKNTRDNYFIHAIDRIYINTSKGLFPESELVAWG"
BASE COUNT         1546 a          403 c          627 g         1085 t
ORIGIN      
        1 taaatatgtt gccaaaatag atagtgaaac atctaaatta tgtcattcac tcaacggaaa
       61 aatatttaaa gttaaagata tgataccagg tgtgaatgcg ccacctatgc atccttggtg
      121 tagaagtacc acagtgccac atgttggcaa ttggcgagac aagttcttta aagagcgtga
      181 aggtaaatat caagtagaag taaaagaagc aaaattacag gaaaaagcta aaaaccagat
      241 gaaagaaatg attgaaagtg gtaaaataaa aatagaaata aatcctgaaa aacagaatag
      301 acattcttta ggtcataaat tatatttaaa aaataaaatt tatgcattgc agaatgatga
      361 gaggtttcct agctatacaa tactctctat agaagaatta aatgatttat taaagaaata
      421 ctcaatgact ggcaaaatat tagtggataa atttggattt aatcgtaagg aaataattaa
      481 tttcgaaaaa actataggaa aagcttatgc aggtggaaaa tatattaata ctgcatatgg
      541 taaaatacat tattctaaaa ctggttctca tatagtacca tttgtaagta aggagaagta
      601 aaatgttaat caaagatgta tatcgtaaaa atgttatagt tacactaaaa aatgatgaaa
      661 aattaaaagg ttttgttata gattttgaaa atccgttaga aagtgattct ggtaactatt
      721 gcatggattt agaaacagat ttaggttttt attctattga tgaatcagat attaaagaca
      781 tacaaataat tacagaatag catcctttct acacagataa agagaaagtg gtgctatttt
      841 tatacatttt tttaaccttc caatgtgaag gttatttttt attgtccaaa acgtgctgat
      901 gacattttaa aagcaagtat ggaatatcag tcgacagact ataaacggag gtatatctca
      961 tggaaaaaaa tgaaagtaat attactgatg taactcagaa cgaagagcaa ctagacaaca
     1021 gtgatgaaca atcacaacag aatgagaaaa cattttctca agaggaagta tcacaattga
     1081 ttaaagagcg tatagctaga gaacgcaaaa aatcagatga acgtattaaa aatgccaaag
     1141 aaaacaatga tagcaatgaa gtagcttatt tattaaaagg tgctaaagtt acaaaagtat
     1201 atggcgatca aaatagtgta tcttttgtgc caggggaaaa agcaaccgaa ttattatttg
     1261 acagtaaacc gaattcaatt gttatgttac ataaccatcc tggacagtca ggattttcat
     1321 tgaatgattt agcagttttt actattaata attctattaa gactatgaca attgtaacta
     1381 ataaaggacg tattaaattt ataagtaaga cagaacattt taaagaaaaa gtaatgaaaa
     1441 aaatgatagc aaacttatta atagaaaaat ctctagatgt aattagtaca aaagatattg
     1501 aaaggttatt aaaagagtta tacaataacg ataatataat ataaagaaat aggtgattaa
     1561 aaggtgacaa tgatgttaga cggtaagtta tctaaaaaag aattgctcag gttgttaact
     1621 gaaaaaaacg atgaaaaaaa taagggaaaa gagaaacaat ctaaatagca tcctttctac
     1681 acaaaaatat agaaagtggt gctattttta tatgcttttt taactattta ctgaaatagc
     1741 agaatcacaa aagatagctt atcttaaaga tttaggcgaa gatggcgaat ataaatatgt
     1801 tgccaaaata gatagtaaaa catctaaatt atgtcattca cttaacggaa aaatatttaa
     1861 agttaaagat atgataccag gtgtgaatgc gccacctatg catccttggt gtagaagtac
     1921 cacagtgcca catgtcggca attggcgaga caagttcttt aaagagcgtg aaggtaagta
     1981 tcaagtagaa gtaaaagaag caaaattaca ggaaaaagct aaaaaccaga tgaaagaaat
     2041 gattgaaagt gataaaataa aaatataaat aaattgtggg aaataaaata gatatatgtt
     2101 aggtcatcac ttatataatg aaaataaaaa aaagagccat tttaaataaa aagaaattgc
     2161 ctagctatac aatactttct atagatctat tgagtgaatt gttaagagaa aaatgtcaac
     2221 aggcaatcta atattaagtg atgagcgatt tgatttgaaa gatattatta attttaatca
     2281 aattattgga aaaatacata tcggaaatgt gtatattgaa accataaagg aaaaagtgca
     2341 ttattcgaag acaggtgctc atatagtacc ttatattgat aagtaggtga aaagtattga
     2401 gaattgaaga tgcatatcgt aaagatgtta ttatcacgct tttgaataat gaagaatacg
     2461 aagggtttgt aactgactat gaaaatgaat ttgagagtga aacaggaaat cttgttgtag
     2521 atatacagac cgattttgct gtttattcgt ttgatgaaac tgagataaaa agtattagat
     2581 tattaaaata atttctaaat agcatccttt ctacacagat aaagagaaag cggtgctatt
     2641 ttttgcgctt ttataactat ttactgaaat atcagaatca caaaagttaa catatttaaa
     2701 agaacttggt gaagatggca aatataaata tgttgccaaa atagatagta aaacatctaa
     2761 attatgtcat tcactcaacg aaaaaatatt taaagttaaa ggtatgatac caggtgtgaa
     2821 tgcgccacct atacatcctt ggtgtagaag taccacagtg ccagatgtcg gtaattggcg
     2881 agacaagttc tttaaagagc gtgaaggtaa atatcgggta gaaggttctt ttattgaatc
     2941 gggtgcactt aataataaaa gtgatgaata cggtatcaaa aggaacaggc atgctcaaat
     3001 ttattataat tcagttagaa atcaagaaaa cagatagaga tttctaaaat tgctaaaaat
     3061 actaatataa ataaaaatac aattcaaaga gtttatgaac atatatttga aaataagtat
     3121 ttattagaaa atggatttaa acaatttgat cctgactttt atatggctca aagttggcaa
     3181 cgtttaagag aagggaaaaa tattaaaaaa tggatataat aatgttgaaa catgaagctc
     3241 ttgaacacta tctaatgaat aagtataatt tacactatat agaggcacat aaacttacag
     3301 aaataaaata taattatagt attttaatta attagaggtg gaaatgattg ctaatattaa
     3361 aaataaaaga aattaatgat aaaagtgtta catataaata ttttcctaat aatgatgaaa
     3421 atattaagcc gggtattatt caaatggata tagatagcct tgaagttatc aatgctgaaa
     3481 aatctagttt agaaaaaaat acaagagaca attattttat tcatgccata gacagaatat
     3541 acataaatac tagtaaaggg ctatttcctg aatctgaact cgtagcatgg ggataaagaa
     3601 agtttacttt taaaaatttc atatttaatt ttaaaaattt taaccttcca atgtgaaggt
     3661 t
//
LOCUS       DAKSQE010000168         3597 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.170, whole genome shotgun sequence.
ACCESSION   DAKSQE010000168 DAKSQE010000000
VERSION     DAKSQE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.170"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..346
                     /locus_tag="O0V33_002554"
     CDS             <1..346
                     /locus_tag="O0V33_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001798264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCX8977144.1"
                     /translation="KVISFIKINDSNYRLNNVDTMKVTLYSNGSNYTKETLLINKDEF
                     CPLRKLILNNKLDSQRVMEVDSLAAIINLVELGKGMTLLPMAFENKSDIVQESSEIFD
                     VSYYTYNNILHK"
     gene            1076..1634
                     /locus_tag="O0V33_002555"
                     /pseudo
     CDS             1076..1634
                     /locus_tag="O0V33_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181351.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            1789..2271
                     /locus_tag="O0V33_002556"
     CDS             1789..2271
                     /locus_tag="O0V33_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977145.1"
                     /translation="MKKFNNWILNAISGSQTDKNGTTEELKGAKFIILYAYSMLVLLA
                     IVISNIFIHILEPKLSITTQIIIVLILIESLIGLRFLKGYDVRRGKDKENKKNSKDFV
                     KLKSILVAILFTSLVLTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVSYTMEEKK
                     "
     gene            2476..3462
                     /locus_tag="O0V33_002557"
     CDS             2476..3462
                     /locus_tag="O0V33_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HCX8977146.1"
                     /translation="MKKCIKTLFLSIILVVMSGLYHSAHASDSLSKSPENWMSKLDES
                     KHLTEINIPGSHDSGSFTLKDPVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMI
                     SVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYY
                     YNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWTDNATFE
                     TKINNGSLNLQVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNLYVNFLSVASGGSAFN
                     STYNYASHINPEIAKTIKANGKARTGWLIVDYAGYPWPGYDDIVSEIIDSNK"
BASE COUNT         1347 a          428 c          661 g         1161 t
ORIGIN      
        1 taaggttatt tcattcataa aaattaatga ctcaaattat agacttaata atgttgatac
       61 tatgaaagta acgctttaca gtaacggaag taattatacg aaagagactt tactaataaa
      121 taaagatgag ttttgtccat taaggaagct tattttaaat aataagcttg attcacaacg
      181 ggtaatggaa gttgattcgt tagcagcaat aataaattta gttgaactag gaaaaggaat
      241 gactttatta cctatggctt ttgagaataa aagcgatata gtgcaggaaa gctctgagat
      301 atttgatgtt agctactata cttataataa tattctgcat aagtgattaa tcgaaagttt
      361 atggggtttt aattatagga acgatattaa aagtctagta gttagaatga gcgatgtgac
      421 agaacagaaa atgtaccttt tatttggtaa atttgttatt ttaaagtagt gatttttgta
      481 gataggaaaa acgggtcctc ttatccgcct ttaccactct ttaaattatg actgcaacta
      541 ctaaaaattg atcgcaaaac ccaacataaa ttgccatact caactatttt ggaaagcatc
      601 attttcatat tatcaaatta taagaaaaag ctgcttcatt ttattgaaga ggaatttaag
      661 aaaaaagttc cgacacgtgt tgctactgaa aaaggcttag actacgactt atatattaat
      721 tttattagta acggtattat cggactacta gatatctatc taaacagtag tgaaaataca
      781 gaagaacaag aaaatataac attgcaggtg tcgcacctac tatctttgta tactctaatt
      841 taaaactaag ctgccaatat tgaatattat ggggtataga caagttatat gtagccattt
      901 tttgagagtg ttttattata atagttttat gagttgatac tgcagtgaca agagctaaaa
      961 aggaatttta tgtaattggt gacatgcaaa gaatacagac aaaaccattt tatgaaacca
     1021 tttttaaaga aatgtagaat aacatacaaa aaagtatata gagggagtta ttttcttgca
     1081 aattagaaat gattttatag aaaagctatt agacagagct caaattaaag aaggtatgcg
     1141 tgtactggat ataggttgtg cgactggtga ggtcacttaa ttaattgcaa aacgtgtggg
     1201 ggcgaatggt gaagtcgtcg gtgttgatgt gaatgaatca ttacttaaaa ttgcaaatga
     1261 aaacaatcaa tataacaatg tattatatca atattctgat atatatcaat tgccagaaac
     1321 tatggggcat tttgatgcga ttgttggtag aagagtattg atgtacttac cagatgctga
     1381 aaagtgttta caaattttga aatcaatttt aaaacctgag ggtatattat gttttcaaga
     1441 aagtgattca aacgtcaatg gataatgatt tagcatggct tgtaggtgta atgctacaaa
     1501 gaatgaaagc acatggtgtc attaatgatg atttttcact agatgagttt aaatcaaacc
     1561 ttgaacacga agcaataaac aatcagtgtg catttatacg tgacatggcg tttggtatta
     1621 tcggaaaagc atagtttaaa aaaagctgaa agtagcatat tattatcaac agtgttttaa
     1681 tttgtgaagt tattaatgga atttgaaatg acagcatttc tataggctaa tcaagtgata
     1741 cttaattact gagcaattta tacattttaa aaggagtaaa attgaataat gaagaaattt
     1801 aacaactgga tattaaatgc aataagtgga tctcaaacag acaagaatgg aacaactgaa
     1861 gaattaaaag gggcaaaatt tatcatttta tatgcatatt caatgctcgt tttgcttgcg
     1921 atagtaattt ctaacatatt cattcacatt ttggagccta aattatcaat caccactcaa
     1981 atcatcatcg ttttgatttt aattgaatca ctaattggac tacgtttctt gaaagggtac
     2041 gatgttaggc gtggcaaaga taaagaaaat aagaaaaata gtaaggattt cgttaaacta
     2101 aaatcaattt tagtagcaat tttatttaca tcattggtgc tgacagcagg cactgtagct
     2161 gatatatacg gtttcactga cttaggaaat actagaagtg atttaatcgt ttggagcata
     2221 ggtggtatta tatttggcct cgtaagttac acaatggaag aaaaaaaata acgataagga
     2281 gctggcgatt ataaagctag ctcctttttt aacttatata tgtaaagaac tatcctaagg
     2341 gtttttaatc atatgtcaat aattcctata atacattatt aaaacatcaa ttaaataagt
     2401 tttaaaattt tacacatatt tttatttaaa aaagatgtat aattaatgta ttaaatatag
     2461 aaagaagttg atattatgaa aaagtgtatt aagactttgt ttttaagtat cattttagta
     2521 gtgatgagtg gtttatatca ttcagcacat gcgtcagatt cgttgagtaa aagtccagaa
     2581 aattggatga gtaaacttga tgaaagcaaa catttaactg agattaatat accgggttca
     2641 catgatagtg gctcattcac tttaaaggat ccagtaaaat cggtttgggc aaagactcaa
     2701 gataaagatt accttacgca aatgaagtca ggagtcaggt tttttgatat tagaggtaga
     2761 gcaagtgctg ataatatgat ttcagttcat cacggcatgg tttatctgca tcatgaacta
     2821 gggaaatttt tagatgatgc taaatattac ttgagtgctt atccaaacga aacaattgtg
     2881 atgtctatga aaaaggacta cgatagcgat tctaaagtta cgaagacatt tgaagagatt
     2941 tttagagaat attattataa taacccgcaa tatcaaaatc ttttttatac aggaagtaat
     3001 gcgaatccta ctttaaaaga aacgaaaggt aaaattgtcc tattcaatag aatgggaggt
     3061 acatacataa agagtggtta tggtgctgac acgtcaggta ttcaatggac agacaatgcg
     3121 acatttgaaa cgaaaattaa taatggtagc ttaaatttac aagtacaaga tgagtataaa
     3181 gattactatg ataaaaaagt agaagctgtt aaaaatttat tggctaaagc aaaaactgat
     3241 agtaacaaag acaatctata tgtgaatttc ttgagtgtgg cgtctggagg cagcgcattt
     3301 aatagtactt ataactatgc atcacatata aatcctgaaa ttgcaaaaac gattaaagca
     3361 aatggaaaag ctagaacggg ttggctgatt gttgactatg cagggtatcc gtggcctgga
     3421 tatgatgata tcgtaagcga aattatagat agtaataaat aaggattcaa taatgatatt
     3481 aagacgagta tgaaaatagt tagattctaa ttattttcac tactcgtttt tattttgaaa
     3541 ataagtaatg attcaacaat attataaatt gaacagattg tttgtgaaat ttttgat
//
LOCUS       DAKSQE010000169         3589 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.171, whole genome shotgun sequence.
ACCESSION   DAKSQE010000169 DAKSQE010000000
VERSION     DAKSQE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.171"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..238)
                     /locus_tag="O0V33_002558"
     CDS             complement(<1..238)
                     /locus_tag="O0V33_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCX8977147.1"
                     /translation="MTKNEIRKYILHKMKTFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYL"
     gene            complement(447..596)
                     /gene="rpmG"
                     /locus_tag="O0V33_002559"
     CDS             complement(447..596)
                     /gene="rpmG"
                     /locus_tag="O0V33_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCX8977148.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
     gene            complement(709..2784)
                     /locus_tag="O0V33_002560"
     CDS             complement(709..2784)
                     /locus_tag="O0V33_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCX8977149.1"
                     /translation="MLKRLKEKSNDEIVQNTINKRINFIFGVIVFIFAVLVLRLGYLQ
                     IAQGSHYKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEM
                     LDTAEKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDK
                     QLLSKIGKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSK
                     LPGVNTSMDWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYL
                     EYQYEDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDK
                     QIKKLRSQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGS
                     SVKGGTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVSINDKQALMHSSN
                     VYMFKTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETKGQIE
                     PLTNNPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDDVGP
                     LKKKINGTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEV
                     FQNGEPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGK
                     DDKNKDKDK"
     gene            complement(2904..3503)
                     /locus_tag="O0V33_002561"
     CDS             complement(2904..3503)
                     /locus_tag="O0V33_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCX8977150.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
BASE COUNT         1033 a          654 c          503 g         1399 t
ORIGIN      
        1 ttaaataatc cattttcggc acaaaaatac gtttatgatc cattaaggct tgttcaataa
       61 tagaaaaagt atctacttca tgattaaaag aaagaactag cgcaattgcg tttgcttctt
      121 tgtattcttc agttgcaaaa aattgatttc ttaaccatgt gtctgctttt cgcttttcag
      181 ctttattaaa agtcttcatt ttatgtaaaa tatattttct aatctcattt ttagtcacat
      241 tatcacccac tttattcaat tctattatat cgttaataac aatacatgtc attatgttgt
      301 catgatgaat tatgcgacaa ttctaaaatt tattatttca attgaaaatc ttttatcatt
      361 tcatacgtgt gcacaattaa aaaggtgaaa cctcttattt tgagatttca ccttttttat
      421 tgttaattta aatttaattt taatgattat tttgtttcac ggtgtaaagt ttgtttgttt
      481 tcacgtgaac agaatttctt catttcaaca cgttctggat tatttctttt gttcttagtt
      541 gtaatgtagt ttctgtcacc acattccgta caagctaaag ttacgtttac gcgcatggtt
      601 attcctcctt atcatttcta aatacgactt tattattata ccaacatcta taatatttgc
      661 aaagttaaaa ttttacaacc atgcgctaca taatcgtcag gttaaatttt atttgtcttt
      721 gtctttattt ttatcgtctt tacctaactg cttaaagtag tagttaatta catctctacc
      781 taagtctcca cctgttaacc atggtggtgg tacaggctga tttgtatata caattgaaaa
      841 cgctaatttt ggatcatcaa ttggcgcgta tcctatataa gtagagttaa ctcttggctc
      901 tccgttttgg aacacttcag cggtaccagt tttaccagca gtaggcacta ctgtatcttt
      961 aaaactaaca tatccagtac catctttatc attaaatgcc attttgaatc cttcttgaat
     1021 ttgtttgatt tctttttcag tattattaac cttgttcaag acagtgccat taattttctt
     1081 cttgagtgga ccaacatcat ctttattagt tgattcatga atcgttaatc caatgtgtgg
     1141 ttgtattcta taaccatcat tcgctatagt tgaaacatat tgtgataatt gtaatggtgt
     1201 ataggtatca tattgaccaa ttgataaatc tagataatta cctggattat ttgttaatgg
     1261 ttcgatttga ccttttgttt catttggtaa atctatacct gttttcacac ctaagcctac
     1321 ttgattcaat cctcttctta gcttttgggc aggtgaactt atgtctgatg gtaaagccat
     1381 accagaataa taagggtctc ccgctaattt taatgctgtt ttaaacatat atacgtttga
     1441 tgaatgcatc aaagcttgct tatcattaat agatacatgc ccgtttttat taaagtatga
     1501 tcgttttgtc aaaccacctt ggaaatgtaa tggttcatcg accattgttt ctccaacttt
     1561 gatagcttta ttctgataac cagctaataa tgttccacct tttacagaag atccaactgc
     1621 aaattgagaa gtaaacgtac caatgtcata atcagtcatt ttaccactct tattaatctg
     1681 ctttccggca agcgcaagaa tgtctccatt tttaggattt tgtacaacca tcatagcatt
     1741 atccatatct tttgcacctt ggctgcgaag cttcttaatt tgtttatcta ataatgcttc
     1801 tacttctttt tgaagatcta tatcgatcgt caatttcaaa tcttgaccgc gagcaccagg
     1861 atttaacact tctgaagatg taactttacc agatttgtcc gttgtgtatt tcatttcttt
     1921 cttcttacca cgcaatacat cttcatattg atattctagg taagattttc caacacgatc
     1981 attgcgtgaa tatcctttgg ataagtaatg ttctgtcaat tctttgggaa taccttcagc
     2041 aggtgtcgat acatctccga atatacctct taaagtgtcg ccatatgggt attttctatc
     2101 ccaatccata gacgtgttaa cacctggtaa tttggaaagt tgctgagaaa ctgctgcata
     2161 ctctttttca ctgacatctt cattttttat catttgtgga tctaaaactg ttcctgcatt
     2221 catctctcga aaaatggcta aaacttgtaa atctttagaa gacaattcat ctaattgtga
     2281 ttttccgatt ttcgataaca gttgtttatc atattgatct tgtttaatac ttccatctgc
     2341 taacatagct tgttcttttg tcatcattgc ttttgctttt ttaggatgca actgaatcca
     2401 gaaatctttt ttatcacgtt ctgtaatctt cttagtatcc atcttgatta gctttgataa
     2461 cttttcagcc gtatccaaca tttccgattg tgttgttttt cgacccctag tatatgtaat
     2521 agccatttta gaagcattat caactaaaac tttcccattt ctgtctaaaa tacgacctct
     2581 tggcacagac tcattcactg taatgttttc atcatttttt ataatttgtt tataatgtga
     2641 gccttgtgcg atttgtaaat aacctaaacg tagtactagt actgcaaaaa taaatacaat
     2701 cacaccaaat ataaagttaa ttctcttgtt aattgtattt tgaacgattt catcatttga
     2761 tttttctttt agtcttttta acaaaactac ctacctctat tcaaagtctt tcactttaaa
     2821 tcatatatga atttagaaat tatttctatc tttttgacaa aaaataacgg tctcatttaa
     2881 gagaccgaac aagtaatcat actttatttt gttgcattat ataattcgtc aactttttcc
     2941 cagttaacta cattccaaaa tgcgccaatg tagtcagggc gtttgttttg atattttagg
     3001 taataagcgt gttcccatac gtctaaacct aaaataggtg ttttaccctc agttaatgga
     3061 ttatcttggt ttggtgtagt cacaatttct aactggccat tgtttacgac taaccaagcc
     3121 caacctgaac caaagcgtgc agctgctttg tcagcaaatt cttttttaaa ttcttctaaa
     3181 gaaccccatt gttctttaat tttttctact acagtacctt tttcttctga gtttggtgaa
     3241 agtaactccc agaataatga atggtttaaa tgtccaccgc cattattacg tacagcagtt
     3301 tggatgttag ctggtacact gtctaaatta gcaacaattt cttcaataga tttagattct
     3361 aaatctgtac cttctactgc agcatttaat ttcgtaacat aagtgttatg atgtctgtca
     3421 tggtgaattt ccatagtttc tttgtcaaaa tgtggttcta atgcatcaaa tgcgtatggt
     3481 aattttggta attcaaaagc cataaataat catcctccta aaatgtctgt aagtaaataa
     3541 taacaagcag tgactggttc aacaaagaat ttgcttaaat tctactact
//
LOCUS       DAKSQE010000170         3544 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.172, whole genome shotgun sequence.
ACCESSION   DAKSQE010000170 DAKSQE010000000
VERSION     DAKSQE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.172"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(32..898)
                     /locus_tag="O0V33_002562"
     CDS             complement(32..898)
                     /locus_tag="O0V33_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCX8977151.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWCTIFVDKRMDHLKDELLNRGLWD
                     ANDIKVYDKVRRQIVAELEKHQSKPSLLHGDLWGGNYMFLQDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVT
                     HSMDKILQDTTD"
     gene            1116..2180
                     /locus_tag="O0V33_002563"
     CDS             1116..2180
                     /locus_tag="O0V33_002563"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCX8977152.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTTFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEYAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     TRYLKDHHFDNVSDIIGLDA"
     gene            2335..2610
                     /locus_tag="O0V33_002564"
     CDS             2335..2610
                     /locus_tag="O0V33_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977153.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQPEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLIIVILAIIRFVHKMM"
     gene            complement(2796..3518)
                     /locus_tag="O0V33_002565"
     CDS             complement(2796..3518)
                     /locus_tag="O0V33_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCX8977154.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
BASE COUNT         1178 a          678 c          532 g         1156 t
ORIGIN      
        1 tgcatattat tatcttaatc taacgtaata actaatctgt tgtatcttgt aaaatcttat
       61 ccatagaatg cgtaacacta tcacggtaca tctccccaaa tttcaataaa tgtaccatca
      121 ataaatataa acgataaaat tcaagtctat aggatgcacc ttttgcgagt ggataatgtt
      181 tattatacgc atcataaaat tcgctcgtaa aaccaccaaa taccgttgta ataccgatat
      241 caaattctct gtcaccatat aatggtgctg gatcaaataa cgccggacga ccatcttgta
      301 agaacatata attgccaccc cataggtcac catgtaataa agatggttta ctttgatgct
      361 tttctaattc cgccacaatt tgacgtcgca ctttgtcata tactttgata tcgttggcat
      421 cccatagccc tctatttagc aactcatctt tcaaatgatc catgcgcttg tcgacaaaaa
      481 tggtacacca atcgtcttgc caatgattat caaaagaaat atcgccacct tcataaggta
      541 atgagaagcc aaatttgcct tcttcttgtt gctgactgtg taattgagct acgagttgcc
      601 ctaattggcg ttgactccct gaagcccctt cttctaaata cgtcatcact aaatacgcat
      661 caccgttaac ctcgccactt gcaattactc taggtgccgt gatacctgca cgttcaaatt
      721 catttaaacc tgcaatttct gcagcataaa atgattcttt acgtccacgt tggacaagta
      781 agaaaaatgt atccgtatcc gtttcgactc gatatgcttc gtttacatcg ccaccactca
      841 ctggtgaaat ctctttaata tcttttaaag gtaaatgctc taaccattgt tcattcatac
      901 aaacaccttc cattacgctt actttatact attatattgc ctaattagga tgatttaatc
      961 tcaattttat ttgtggtact tgcgtcaatt ttcaaaactt tttagtttta actaggaaat
     1021 tatgtaggga aatgctagtc tattaaaaga atatatttca catatattaa gttgaggata
     1081 gtaagcctca aatataatga aataggagca tactcatgta caaattaatt aaacctttct
     1141 tattcaaaat cgaacccgaa aaagcacacg gactaactat cgatgcatta aaaacgttac
     1201 aaaaatttcc agttttattc ccagtcgtcg ataaactatt tacttataag aatccaacgt
     1261 tatcacaaac gatacaaggt aatacgtacg acaatccaat tggcttagca gctggtttcg
     1321 acaaatcttg cgaagtacca aaagcattgg aacaccttgg attcggtgct ttagaattag
     1381 gtggtatcac acctaaacct caaccgggta accctcaacc acgcatgttc agattattag
     1441 aagatgatgc cttgataaat cgtatgggct tcaataatat tggtatgaac aaagcactca
     1501 gtcatttgcg taaaaatgct tatcaagtac ctgttggtat caatgttggt gtgaataaaa
     1561 tgacacctta tgaagcgcgt tatcaagatt atataaaagt cattgatacg tttaaacacg
     1621 acgtttcatt tttcacagtc aatatcagtt ctccaaatac tgaaaacctt caaaatttcc
     1681 atgataaaga tgaattttca atgttatgtc aagctttgac aacatttaaa aaacagcacg
     1741 atgtaacagt gccaatttac ttaaaactaa cgtctgatat ggatttcgat ggcttaaaag
     1801 cactattacc agcgattact gagacatttg acggtatcat cttagcaaac acaacgcgac
     1861 aacgagatgg tttaacttct gctaataaag tcgaagaagg cggtttgagt ggtcgtccat
     1921 tatttgaacg taatttaaaa ttgattaagt atgcttatca gcaaacaaat ggtgaatttt
     1981 taattatagg tacaggcggc gtattcagta ctgaatatgc aatcaaaatg atgcgtcacg
     2041 gtgcgtcact tattcaaatt tattcatcac ttgttattga aggcccaggt ttaactaaga
     2101 aaatgaacaa aggcatcaca cgttacttaa aagatcatca ttttgacaat gtcagtgata
     2161 ttataggact agatgcctaa tccaacgtag tcaaataaca ctttaaaact tgctgaatcg
     2221 tcttattaaa attatgatat aactcaagaa gatatattta tcaccatttt tcaaaataat
     2281 ggcttaaata tcacatatca ttaaatatag cgttttataa cggaggcgtt tgttatgact
     2341 aaagaaacag ataataaaaa aacagtagcg acttctgaca acaaagacac acagcaacaa
     2401 aatcaacctg aagaagatga acgtcaaggt ttgaatggtt atcgtaaaac agatcttgat
     2461 ttagaaatcg agcaagagct acgtgaaatg atgaaaacag gagaaaatga aacgaaaaat
     2521 gactacaaaa agtttaaagt gttttcactt atttcaacac ttatcattgt cattttagca
     2581 attataagat ttgttcataa aatgatgtaa ttagagtgag acattgtttt atgtctcaga
     2641 atccagttat tcatgatagc tacaatattt acgatgatat aaataacccg agattttagt
     2701 atgattcatt tcactaaagt ctcgggtttc tatttgataa tttttaatag ggcatggctt
     2761 gtatatgcgc ctgcctttta tcttatttcc aatgattaat ctggatattg ttctaaaaat
     2821 gctttcgctt ctttattaac tgttttaaaa tcaatacctt gttgcatcgc tgcaaagaca
     2881 catccacaat aacactgcct aaagatatta tagtcattac acatttctat ggaacgctca
     2941 taacctttac ttttcttaaa atcacttggc aaatagttca catcgtatat tttttggaca
     3001 tccataccaa gttcattgat taattgtgcg ttctttttag gtgataacgt gattgcactg
     3061 ccaaaataat catagccatg ttctacagct gctttcgcta caatatccaa acgcatttca
     3121 aaacaagcgg tacaacgtaa gccgccttct ttttcatcag ctaattcttt atccttcacc
     3181 attttcacaa acttatgcgg ttcataaggt gcttcaatat acttcacatt cgcaccagtc
     3241 ttgcgattaa aatcttccac aaattgttct tgcactttag cacgtcgtaa atattcattt
     3301 ttcggatgaa tatttgaatt cgcgaaataa attgcaatgt ctgcatattg tgttaaaaac
     3361 tctaatgtat atgtactaca aggtgcacaa caactatgca ataagatttt aggtctgatt
     3421 gcttctcttt cccactgccc gattaatttc ttcaacacct tgtcataatt aattttttga
     3481 tttttcattt tactgataat aggttcagca ttaatcatga tttaacctcc cacatttaat
     3541 catt
//
LOCUS       DAKSQE010000171         3426 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.173, whole genome shotgun sequence.
ACCESSION   DAKSQE010000171 DAKSQE010000000
VERSION     DAKSQE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.173"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            187..1230
                     /locus_tag="O0V33_002566"
     CDS             187..1230
                     /locus_tag="O0V33_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCX8977155.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYGVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
     gene            1233..1913
                     /gene="sdaAB"
                     /locus_tag="O0V33_002567"
     CDS             1233..1913
                     /gene="sdaAB"
                     /locus_tag="O0V33_002567"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCX8977156.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIVEEE
                     GDSIGQHPNCAYIKSFRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIMIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            1927..2826
                     /gene="sdaAA"
                     /locus_tag="O0V33_002568"
     CDS             1927..2826
                     /gene="sdaAA"
                     /locus_tag="O0V33_002568"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCX8977157.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            2845..2943
                     /locus_tag="O0V33_002569"
     CDS             2845..2943
                     /locus_tag="O0V33_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977158.1"
                     /translation="MIKKKHLSGSLVPDRCFLIIFHKVEKFMGNAC"
     gene            3005..3175
                     /locus_tag="O0V33_002570"
     CDS             3005..3175
                     /locus_tag="O0V33_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977159.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
BASE COUNT         1148 a          512 c          719 g         1047 t
ORIGIN      
        1 ttagtaaata ttaatgacaa cataagcagt ttttgaaaca atccatttta aaaatctttc
       61 acaaatatga aatatttcac ttaaatattt gatttgataa cagataaaag ttgaaaacgt
      121 tttcttaagg gtgtacgatg tttatggtct gaaaataaaa taaacataga gagaggtttt
      181 tgggaaatgg atattctgtt aggtgtaggc acattagtct tggttttagt gatcatgacg
      241 ctatttttaa agtatgcacc gtacggtaaa caaggactac aagcattatc cggtgcagct
      301 tgtgcgacat ttctaccaca agcattctta agttacgcta ttggtggcgt atttgatatt
      361 aaatttctac aagatattgg ggatttagca ggaagtttaa gcggtatcgc agtaggtatt
      421 ctaacgtgct taaacttagg cgttgcacca gtatttgcgg tcattgttgg tttagtatta
      481 catgacttta aattattacc agcattcatc gcagcttatg gcgtagcttt tttaattaaa
      541 tggattgaaa agaaagttcc agaaggcttg gacttaattg tggtgatctt atttgcacca
      601 gcaattgctt tcggacttgc atcaatcatt acacctggtg tgctagcaac attaaaacaa
      661 ataggtagtg ctgtcactgc ggtaggtgat aacaatccat atgcattagc ggtcatttta
      721 ggactagtga ttcctgttac aggtatgaca ccgctaagtt caatggtatt aacaagttta
      781 ttaggactga caggtgttcc tatggctatt ggagcactaa catgtacagg tagctcattt
      841 gttaacctta ttttattccg aaaattaaac attggtggac cgtcaaaagc atttgcagtt
      901 tgtattgagc cattgacgca aattgattta atagcgcaat atccagtaca actatatggt
      961 acaaatgcac tgattggggt cgtcaacgca tgtatcgtga catttagcgg actagtaata
     1021 ggtgtcaaag gtatggcaac accaattgca ggtgccatcg tattatttgg ctttaataat
     1081 gcagtgacat caattgtaac aatcgcaaca gtgattattg tcagcattgt cttagcctat
     1141 attattggaa cactaattaa taagtttaat ttaatgaata tcaactttaa aatgccgagc
     1201 aaaaagaatc atattaagga gagtgtttaa ttatggcaaa gagctatgat tatcaaagtg
     1261 ctttcgatat tatcggacca gttatgatgg gaccttcaag ttctcatacg gctggtgcag
     1321 taaagattgg gaattcagca cgcgcaatat tgggagatat tcctaaaaat attgaaatac
     1381 attattatga atcatttgct caaacacacc aaggacatgg tacagatgtc gctattgtgg
     1441 gtggagcaat gggatattca acatttgata accgaattaa atcttcatta gaaattgcag
     1501 ctgatgatgg tattaaaata gatatcgtcg aagaagaagg cgatagtatt ggtcaacatc
     1561 caaactgtgc ttatattaaa tcatttcgta atgatggtcg ctacattgaa attatcggta
     1621 tttcaatcgg tggtggtaca attaaaatta aaggtattca tataaatgga ctagaagtag
     1681 atttaaatca tggattacca attttagttg ttgacggaaa tatgactaaa gcacaagtaa
     1741 atcatttcat taatgatatc aacgatatga aattagactg taaagatgaa tttattatga
     1801 tagatgaaga taaatgcttg gttgtaatac cattaaataa agcaatctca gaatcgacat
     1861 taaatacaat taaagagaaa tacagtgacg taaacgtttc ctatataaat tagaggggga
     1921 ataaacatgt ttgattcaat tagagagact atagattatg ccgtagaaaa taatatgtca
     1981 tttgcggata tcatggttaa agaagaaatg gaattaagcg gtaaatcacg tgatgaagtg
     2041 cgagcgcaaa tgaaacaaaa tttagatgtc atgcgagacg cagtaataaa agggacgaca
     2101 ggtgatggcg ttgaaagtgt aacgggctac actggtcatg acgctgctaa actacgtgat
     2161 tataatgaaa cacatcatgc tttgtctgga tatgaaatga ttgatgcagt caaaggtgcc
     2221 attgcaacaa atgaagtcaa tgctgcgatg ggtattattt gtgcaacgcc aacagctggt
     2281 tcctcgggta ccattcccgg tgcacttttt aaattagaaa aaacacatga tttaacagaa
     2341 gagcaaatga ttgacttctt attcacttca gcattgtttg ggcgtgtcgt agcaaacaat
     2401 gcaagtgtag ctggtgcaac aggtggctgc caagctgaag ttggttcagc atctgcaatg
     2461 gcggcagctg cagcagtagc tatattcgga ggatcaccag aagcatccgg gcacgctatg
     2521 gcattagcga taagtaattt attaggttta gtttgtgacc cagtagccgg acttgttgaa
     2581 attccttgtg ttatgagaaa tgcaattggt tcgggtaacg ctttaatttc agcagattta
     2641 gcattagcag gtattgaaag tagaatccct gttgatgaag ttattgaagc aatggataag
     2701 gttggtcgta accttcctgc atcattacgt gaaactggat tgggtggact agcaggcaca
     2761 ccaactggcg aagcaattaa acgtaaaatc tttggcacag ctgaagatat ggttaaaaat
     2821 aattaatgag ataacaataa aatattgata aaaaagaagc atctgtctgg atcattagta
     2881 ccagatagat gcttcttaat tatctttcat aaagttgaaa aatttatggg aaatgcatgc
     2941 taatcaaaat agtgattgca aaagcgcccc cgtatatcta aaataactta aaaggagtga
     3001 tgatatgcta agactatcaa tcatctttat agcattcatc attaacacaa cgattacata
     3061 tggatatacg actgagggaa cgtgggtcaa tctattattt aaatctttat cgctcagcat
     3121 gattatcgtt ttcatgttct actatattcg ttttgttatt gagaaaaagc gttaaatcaa
     3181 agctatgggg atagtatttg aaaagaataa ataaaaggga cgatgtaaat caatgtaata
     3241 tgctctacaa agttatattg gcagtagttg actgaacgaa aatgcgcttg taacaagctt
     3301 ttttcgattc tagtcagggg ccccagcaca gagaatttcg aaatgaaatt ctacaggcaa
     3361 tgcgagttgg ggtggaacga cgataaagaa atactttttc tatagaaaaa gtatttctta
     3421 tgcatg
//
LOCUS       DAKSQE010000172         3347 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.174, whole genome shotgun sequence.
ACCESSION   DAKSQE010000172 DAKSQE010000000
VERSION     DAKSQE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.174"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            78..1253
                     /locus_tag="O0V33_002571"
     CDS             78..1253
                     /locus_tag="O0V33_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HCX8977160.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISTMEQDKIKYATQTICQDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            1231..2421
                     /locus_tag="O0V33_002572"
     CDS             1231..2421
                     /locus_tag="O0V33_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCX8977161.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHIVWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAVFYGSYFIANR
                     EKAK"
     gene            complement(2534..3043)
                     /locus_tag="O0V33_002573"
     CDS             complement(2534..3043)
                     /locus_tag="O0V33_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HCX8977162.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIE
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
BASE COUNT         1115 a          533 c          519 g         1180 t
ORIGIN      
        1 gtctttattt cttttataat gccgtaaaat aagttacact taaataaaat tttcaaacta
       61 acggagggtg gctattaatg gttactcaaa ataaaaagat attgattatt actggctctt
      121 tcggtaacgg tcatatgcaa gttacacaga gtatcgttaa tcaacttaat gatatgaatc
      181 tagaccattt aagcgtcatt gagcacgatt tatttatgga agctcaccca attttgactt
      241 ctatttgtaa aaaatggtat atcaatagct ttaaatattt tagaaatatg tacaaagggt
      301 tttattacag ccgcccagat aaactagaca aatgtttcta caaatactat ggacttaata
      361 aattaataaa tttattgata aaagaaaagc cggatttaat attattaacg tttcctacac
      421 cagttatgtc ggtactaact gagcaattta acattaatat tccagttgct acagtgatga
      481 cagactatcg cttacataaa aactggatta cgccgtattc aacaagatat tatgtggcaa
      541 caaaagaaac gaaacaagac tttatagacg taggtattga tccttcaaca gttaaagtga
      601 caggtattcc tattgataac aaatttgaaa cacctattaa tcaaaagcag tggttaatag
      661 acaacaactt agatccagat aagcaaacta ttttaatgtc agctggtgca tttggtgtat
      721 ctaaaggctt tgacacgatg atcactgata tattagcgaa aagtgcaaat gcacaagtag
      781 ttatgatttg tggtaagagc aaagagctaa agcgttcttt aacagctaag tttaaatcta
      841 acgagaatgt attgattcta ggttatacca aacacatgaa tgaatggatg gcatcaagtc
      901 aacttatgat tacgaaacct ggtggtatca caataactga aggtttcgcc cgttgtattc
      961 caatgatttt cctaaatcct gcacctggtc aagagcttga aaatgccctt tactttgaag
     1021 aaaaaggttt tggtaaaatc gctgatactc cagaagaagc aataaaaatt gtagctagtt
     1081 taacgaatgg taatgagcaa ttaactaaca tgattagtac aatggaacaa gataaaatta
     1141 agtatgctac acagacaata tgtcaagact tacttgattt aatcggtcat tcatcacaac
     1201 cacaagagat ttatggaaag gttcctttat atgcaagatt cttcgttaaa taattacgct
     1261 aatcataaaa atttcatttt aatgcttatt atcttatttt taatggaatt tgcgagaggc
     1321 atgtatatcc taagctatat taatttcttg ccaacagtaa catccattgc tgtagcaata
     1381 acgtctctag cattttcaat acattttatt gctgatgcat ctacaaactt tgttatagga
     1441 tttttactga aaaaattcgg cacaaaaatc gttttaacga ctggatttat tttagcattt
     1501 acaagtttat ttttagttat ttggtttcca gcatcacctt ttgtaatcat atttagtgct
     1561 atgatgctcg gtattgcagt tagtcctatt tgggtcatta tgttatccag tgttgaagaa
     1621 gataaacgtg gcaaacaaat gggctatgtt tatttttcat ggttgttagg cctattggta
     1681 ggtatggttt ttatgaatct acttatcaaa gtgcacccaa cacgctttgc cttcatgatg
     1741 tctttagtcg ttttaattgc ttggatatta tattattttg tcgatgtaaa gttaacaaat
     1801 tataatactc gtccagttaa agctcaattg agacaaattg ttgatgtgac aaagcgccat
     1861 ttattgttgt tccctggtat tcttttacaa ggtgccgcaa ttgctgcact agttcctata
     1921 ttaccaacat atgctactaa ggttattaac gtaagcacaa ttgaatatac tgttgcaatc
     1981 attattggtg gtatcggctg tgcagtttcg atgctatttt tatcgaaact aatagataac
     2041 cgcagcagaa actttatgta tggtgttatt ctaagcggat ttattttata catgatatta
     2101 atttttactc tatctatgat tgttaatatt cacatcgtgt ggattatcgc tttagctatc
     2161 ggtctaatgt atggcatctt attaccagca tggaatacct ttatggcacg atttatcaaa
     2221 tcagatgaac aagaagaaac ttggggtgtt tttaatagta tccaaggatt cggctcaatg
     2281 atcgggccat tatttggcgg attaattaca caatttacca acaatttaaa caatacattt
     2341 tatttctcgg cattaatctt tttagtctta gctgtatttt atggaagcta ctttatagca
     2401 aatagagaaa aagctaagta aggttaaaac gtattttaaa ataaagaaaa tggtcatttt
     2461 tattacattc aataaaaatg accattttct tattaattca aacatactac gattaacaat
     2521 actattatgt tacttaagct aatttaaacg tttcaataac tttccattta tcttcgtcat
     2581 cgtcaaaacg taacaaagtt aattcatcga taatttcttt atggtctacc ccagctaatg
     2641 ctacttgacc aaaaatatct tcgaattctt ggctagatag tccttgtgct attgtaaagt
     2701 gtggcacaaa aacatgttca gcttctccat agaaatcttc tccattaaag cgattaaaca
     2761 attcttctaa gtcgtccgtc ttcgcaactt taaagtaaat cacattgttc gttggtttga
     2821 agctagaagc ttttgtagca tgaacttcta ctgctggtat accattaata cgagctctaa
     2881 cctgttcaat gacagaatct aaatcaccat cttcaatttc aaatggtgct ttaattgtca
     2941 catgtggttt aattcgtgaa tactgtttat catatctttt acggtaagaa tccaccgctt
     3001 cttgaaatga ctttgatgga attaatgcta atcctaaaat catctgaatt cctcctttgt
     3061 ttaacctatt gctattatat caaatttctt tgaaaaccgt tacattaaaa ataattcatt
     3121 atacatcttt tgcaatttcg acaaatttaa attaaattaa acagtaaaat cttttataaa
     3181 tatatttaac tatagcattg tcaaatttat ctagacagcc tagataaccc tataaataat
     3241 tttaattctg ttttataatt cagtgcgttt ctattacaat gttcatttaa gcttgttgtg
     3301 ataggattct ataataaaca aaaaatattg atttctagta acagttg
//
LOCUS       DAKSQE010000173         3305 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.175, whole genome shotgun sequence.
ACCESSION   DAKSQE010000173 DAKSQE010000000
VERSION     DAKSQE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.175"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..603)
                     /locus_tag="O0V33_002574"
     CDS             complement(<1..603)
                     /locus_tag="O0V33_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977163.1"
                     /translation="MNNKLIYTSYDGDNILLIDSFIKLVIDFKYIPINPTKSLGYYIS
                     TSIHDNDKGECLRDCLSLEMICDELWVFIDNNKYIPEGVRLEIASWLKYKSSPVKYIS
                     IPSLLENSSINDDLFLDFDDSNILKEKEISEPVPKKSELRPVNCINILPEHHKYIDWI
                     KYHLFYNKFVPLDYLSIKPYIYFDNIEHYKSELSLLNERCN"
     gene            complement(769..1527)
                     /locus_tag="O0V33_002575"
     CDS             complement(769..1527)
                     /locus_tag="O0V33_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCX8977164.1"
                     /translation="MLEFKAGKINKHVLYSDILNRDVTLSIYLPESYNQLVKYNVILC
                     FDGLDFLRFGRIQRTYESLIKEARIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQS
                     VGKEILPFIDSTFSTLKVGNARLLVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDEK
                     VLDKLNQCANKEQLTIWHVIGLDEKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYY
                     DEFEGGHQWKDWKPLLSDILLYFFSNQSPDQLYE"
     gene            complement(1670..3238)
                     /locus_tag="O0V33_002576"
     CDS             complement(1670..3238)
                     /locus_tag="O0V33_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCX8977165.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTVIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGGIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSVFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGSGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
BASE COUNT         1149 a          643 c          436 g         1077 t
ORIGIN      
        1 attacatcgt tcattcaata gcgataactc acttttataa tgttctatat tatcaaaata
       61 aatatatggc tttatactta aataatccaa tggtacgaat ttgttataaa ataaatgata
      121 ctttatccag tctatatatt tatggtgttc cggtagtata ttaatacaat ttacaggtct
      181 taactcactt tttttcggta ctggttcgga aatttccttt tctttaagta tatttgaatc
      241 atcaaagtct aaaaataaat cgtcatttat actcgaattc tctaataaag aaggtatgct
      301 tatatactta actggagagg atttatattt taaccatgag gctatttcta accgaactcc
      361 ttctggaata tatttattat tgtctatgaa tacccaaagt tcatcacaaa tcatttccaa
      421 agacaagcag tctcttaaac actcaccttt gtcattgtca tgaatcgatg tagatatata
      481 atatcctaat gatttagttg gatttattgg aatatattta aaatcaataa caagtttaat
      541 aaaactatct atcaatagta tgttatcacc atcataagag gtatatataa gcttattatt
      601 cactgtaaat tccccccttc actattctat acatttcacc ttcttgaagc tctttatccg
      661 ttgttattgc aggaatatgc ttttctctta atataatacc taagtgccct aaaattgccc
      721 ctcttttgaa gataaaacca ctacaataat cagttatata agaacatatt actcatataa
      781 ttgatccggt gactgattac taaaaaaata caataatata tctgacagca atggtttcca
      841 atctttccat tggtgaccac cttcaaattc atcgtaataa taagttatat tatatttctt
      901 aatttgttca gctaattctc tattcggtgt taagaaatcg gcacgcttac catttgttgg
      961 taaagtgaaa tctttttcat ctagaccaat gacatgccaa attgtcaatt gttctttatt
     1021 agcacattga tttagcttat ctaatacttt ttcatctgaa tgtggactta acattgctac
     1081 acgactaaaa atcgttggat aggtcaacgc cgttaataag gcaatactac ccgctaaact
     1141 atctcctact aataaccttg cattacctac tttcagtgta gaaaacgtcg agtcaataaa
     1201 tggcaatatt tctttaccga ctgattgaat agttaaatga gaacgacttc cttgtggatg
     1261 aaattcttct ctacgcttat caacgtcttc ataatggaat ccgacaatga tcgcatcatc
     1321 aatacgcgct tctttgatta acgattcata tgtacgttgt attctcccga aacgtaaaaa
     1381 atctaatccg tcaaagcaaa gaatgacatt atatttaaca agttgattat aagattctgg
     1441 caaataaata cttagtgtga catctctatt taaaatatca ctatataaaa catgtttatt
     1501 aatctttcca gctttaaatt ccaacataaa aaagtgcccc ctagtaattg tattattatt
     1561 gtaccaagag acactatatt ttaaaattca aatttctgag cacctaaaat tctcaatact
     1621 ttttcttttt tatcaaatga tcactacata taccataact aaaagttgac tactttttgg
     1681 cacgtgcttg ttttaaacgt tctggatatg tcttcaacca aaagacagca ttaggtaatt
     1741 tattcggatc aggttgataa actgcattat aaccgctgcc taaacgtttc ttttgaattt
     1801 cataatcttt taaagcattt acggcaggct tatgtaaaat ccaaatcgca atgatattta
     1861 accaagccat tagacctaca cctaaatcac cgaatgccca tgctacatca gctgttttaa
     1921 ctgcaccgta aaatgtagcg aacaaaataa tcacacgagc aatattaata aataccgatg
     1981 aaacttgatt attttgatta cgcgttaaat aagcaacatt tgtttctgta atgtagtagt
     2041 acgacaaaat tgtagtaaat gcaaagaaga ataaagcaaa tgcaataaag tacgaaccta
     2101 cgccagagaa agtaggatca aattgataac cactgccatg gaacgcttta tcaatgccgg
     2161 cttgtgcata catcgcagta cctgaataat ctttacctgt tgcattttca acataaatcc
     2221 caccgtcttt aattaaatgc ggtgtgccat tcgcattaac cgtaccatca gtcacattat
     2281 atgtaccaga aataagtata atcagagcag ttgcagtaca tacaaataat gtatcaatat
     2341 aaactgaaaa tgcttgtact agaccttgtt tacttggatg tgatacttct gctgccgctg
     2401 ctgcgtgtgg acctgtacct tgaccagcct catttgagta taatccacgt ttaacaccaa
     2461 tctctatcat tgcgccaacg ataccaccaa aagcagattg taatccaaat gctgatttga
     2521 aaattaatgc aaataacgct ggcacttctt gtatattgat acaaatgata atgacagcca
     2581 ttagtatgta aataattgcc ataaatggta caacggctgt tgcaacattg gcaatactac
     2641 gtacaccacc aaaaataatt aatcccaaaa taactacaac aataccaccc attaaccatt
     2701 gtggaacatg aatcgcattg tgcatagaac ttgctatagc gttagattgc acaccaggaa
     2761 gcaatagacc tactgagata atcgtaacaa tagcaaagat aattccgtaa attttaccaa
     2821 atttaccacc aataccatat tctatataat acgctggtcc accacggtat tcattatttt
     2881 caactctctt gaatatttga ccaagtgtag attcaataaa agcactactt gcacctaaaa
     2941 atgcagtaat ccacatccaa aatactgcac caggtcctcc tataaatatt acagtagata
     3001 caccgacaat attacctgta ccaaccctgc ctgctaaaga catggctatc gcttgaaaac
     3061 ttgaaatacc atttggagac ttctctcctt gaaacattaa tcgaatcatt tctttaaaat
     3121 gtctaacttg aaaaaatcgc atacgcaatg taaatagcac accagtaatt agcaaaccat
     3181 aaactaatgg cttactccaa acaacctcat ttaaaaaggt tactaatttt tcaatcataa
     3241 gattccccct attgtttaaa cataaaaata ttatacgatt aagcacgaga cacttcaata
     3301 tattt
//
LOCUS       DAKSQE010000174         3281 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.176, whole genome shotgun sequence.
ACCESSION   DAKSQE010000174 DAKSQE010000000
VERSION     DAKSQE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.176"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            288..2438
                     /locus_tag="O0V33_002577"
     CDS             288..2438
                     /locus_tag="O0V33_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCX8977166.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKKDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVENAIRGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTAVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVAKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     QLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FETPILKSDVLSIEDLKEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWIHSIDKNKDKVSLTMIDPHE"
     gene            2431..2886
                     /locus_tag="O0V33_002578"
     CDS             2431..2886
                     /locus_tag="O0V33_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCX8977167.1"
                     /translation="MNNEILQRMVENLSEEKFGRTFQHRAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
BASE COUNT         1259 a          408 c          630 g          984 t
ORIGIN      
        1 tgtcgcgata tagtattaat aatttaagtg aagaagatat ctaattgtca gtttaattaa
       61 gtggattgct attagaataa aaaaagtagg tttagattat gaaatttagg aaatgatggt
      121 gtgtcatttt caataatctt agtgcgtttt aagatatagt atgacctaat aattcgtttt
      181 taaagttttg gaagttaaaa ttgcattaag tatcatacct taatagaagt attttagaat
      241 atgttaaaat aaatgagtaa atttaagaaa aagtgtgggt taagtaaatg gacaatcaat
      301 tgattaattc aatcatagag aaatatcaat ttagtaaaaa acaaattgaa gcagtgttaa
      361 cactgcttga agaaaaaaat acagtaccat ttattgcgag gtatcgaaaa gagcaaactg
      421 gtggactaga tgaagttcaa ataaagcaaa ttgatgacga ataccaatat atggtcaatt
      481 tacaaaaacg taaagaagaa gttatcaaaa atatagaaca gcaaggatta cttactgagg
      541 aattaaagaa agatatttta aaacagaaca aattacaacg tgttgaagac ctatatagac
      601 cttttaaaca aaagaaaaag actagggcga ctgaggcgaa acgtaaagga ttagaaccat
      661 tagcgatatg gatgaaggca cgtaaacatg aagtatcaat tgaagaaaaa gcacaacaat
      721 ttataaatga agaagtgcaa tcggttgaaa atgctatcag aggtgcgcaa gatattattg
      781 ctgaacaaat ttcagataat cctaaatata gaacaaaaat tttaaaagat atgtatcatc
      841 aaggtgtgtt aactacatct aaaaagaaaa atgctgaaga tgaaaaaggt atttttgaaa
      901 tgtactatgc atatagtgag ccaattaaac gcattgctaa tcatagagtt ttagctgtga
      961 atcgtggcga aaaagagaaa gtattatctg taaagtttga attcgatacg acagcagtag
     1021 aggacttcat tgcacgtcaa gaaatcaatc ataataatgt aaatcgcagt tatattttag
     1081 aggcgattaa agatagcttg aaacgcttaa ttgtcccttc gatagagcgt gaaatccatg
     1141 ctgatttaac tgaaaaagct gaaaatcatg caatagatgt ttttagtgaa aacttaagaa
     1201 atctattact gcaacctcca atgaaaggta aacaaatatt aggcgtagat cctgcattta
     1261 gaacaggttg taaattagca gtcattaacc catttggtac ttttatagca aaaggtgtga
     1321 tttatccgca tccaccagtt gctaaaaaag aagcagcaga gaaggatttt gtacaaatgg
     1381 ttaaagcgta tgatgtgcaa ttaattgcaa ttggcaatgg tacagcaagt cgtgaaacag
     1441 aacaatttgt tgcagattta attaaaaagc atcagttgcc agtacaattc atcattgtca
     1501 atgaagcggg cgcctcagta tactcagcat cagaaattgc tagagatgaa tttcctgatt
     1561 ttcaagtgga agaaagaagt gccgtatcaa ttggacgacg cgttcaagat ccattaagtg
     1621 aattagttaa aatcgatcct aaatctattg gtgttggaca ataccagcat gatgttaatc
     1681 aaaaagcact agaaaatgca ttaactttcg ttgttgaaac agcagttaac caagtaggtg
     1741 ttgatgtcaa tacagcatca tcatcattat tacaatatgt gtctggtcta agttcgcaaa
     1801 ttgctaaaaa tataattgca tatagagaag aaaatggcgc aataaagcat aataaagagt
     1861 taagcaagat taagcgtttg ggtgctaaaa cttttgagca gagtatagga tttttaagaa
     1921 tagtagacgg gtcagagcca ttggataata catcgattca tcccgaaagc tataaagtaa
     1981 cgtatcagtt attagataaa ctcggatttg gtggtaatga tttaggtagt gatgcattaa
     2041 aagctaaatt aaattcattg gatatggatg aactggcgat tgaattacaa gtcggtgtac
     2101 ctacattaga ggatattatt aaatccttga aagcacctaa tcgagatcca agggacgagt
     2161 ttgaaacacc aatccttaaa tccgatgtgc tatcaattga agatttaaaa gaaggaatga
     2221 aattgagtgg cacagttaga aatgtagtag attttggagc atttgtagat ataggtgtta
     2281 aacaagatgg acttgtccat gtttcgaaac tttctaagaa atttgttaaa aacccaatgg
     2341 atattgtcag tgttggtgat atcgtagacg tttggattca tagtattgat aaaaataaag
     2401 acaaagtatc attaacaatg attgatccac atgaataacg aaatacttca gcgaatggtc
     2461 gagaaccttt cggaagaaaa atttggacgt acctttcaac atcgtgcata ttttaataaa
     2521 aggttacgta caacaggcgg acgttatctt ttaaagtctc atgatattga aattaatcca
     2581 aaacaatatg agcattatgg agaagatgct gttgtaaaaa ttatactgca tgaactgtgt
     2641 cattatcact tgcatattgc tggtaaaggt tatcaacata aagatcaaga ttttaaacga
     2701 ttgagccaac aggttggagc gcctagattt tgtaacagca ttgaaagcta tcaacaacgg
     2761 gcaaattatg aatattattg cactaaatgt catgcaaaat atatacgtat ccgtaaagtt
     2821 gatacgaatc gtatgcgttg cggacattgc aatggaaaat taagaatgaa aagacaattg
     2881 aaataagtac aatacaaata gagctaattc atgtatatgt aagattattt atgagttgta
     2941 tggaatttat atagaattta tcagtgagtt attttgattg gtaaacatag taattgaata
     3001 agaagaaatt gtgtttataa aatgtttaaa gccgagattg tcgtaattta atacttcgca
     3061 atcgcggctt ttctactttt aatataggta atggcataaa ttgtgacttg ataggatgaa
     3121 attaaataga ttagattaaa aatcttggcg tattttagta atgaattata tataaacttt
     3181 acccaattaa agctttaaag tggcacaaaa gataattata catttttaat gacattttga
     3241 ttgaatttat ctattgactg tacagcttat agttagtatg a
//
LOCUS       DAKSQE010000175         3266 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.177, whole genome shotgun sequence.
ACCESSION   DAKSQE010000175 DAKSQE010000000
VERSION     DAKSQE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.177"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            36..413
                     /locus_tag="O0V33_002579"
     CDS             36..413
                     /locus_tag="O0V33_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCX8977168.1"
                     /translation="MDKQVRNTTEIVRLAKQKSQKTREKVDKAISKFSIEGKAINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITSNVVSKPKKSSRSEEILIKTLKRRVME
                     LKKENKKLQNQIQKLYGDLYNKE"
     gene            651..1016
                     /locus_tag="O0V33_002580"
     CDS             651..1016
                     /locus_tag="O0V33_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCX8977169.1"
                     /translation="MTKDMCEVTYIHEDKVNRAKKDLAKQNPMDVAKVFKALSDDTRV
                     KIAYVLSLEGELCVCDVANIIESSTATASHHLRLLKNLGIAKYRKEGKVVYYSLDDEH
                     VKQLVEKAFLYQREVASIG"
     gene            1009..3189
                     /gene="cadA"
                     /locus_tag="O0V33_002581"
     CDS             1009..3189
                     /gene="cadA"
                     /locus_tag="O0V33_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase CadA"
                     /protein_id="HCX8977170.1"
                     /translation="MDSSAKTLTEDKQVYRVEGFSCANCAGKFEKNVKQLAGVQDAKV
                     NFGASKIDVYGNASVQELEKAGAFENLKVFPEKLANSSMQAVKEDTKAPKEEKIPFYK
                     KHSTLLFATLLIAFGYLSHFVNGEDNLVTSMLFVGSIVIGGYSLFKVGFQNLIRFDFD
                     MKTLMTVAVIGAAIIGEWAEASIVVVLFAISEALERFSMDRARQSIRSLMDIAPKEAL
                     VMRNGQEIMIHVDDIAVGDIMIVKPGEKIAMDGIIINGVSAVNQAAITGESVPVAKTV
                     DDEVFAGTLNEEGLLEVKITKYVEDTTISKIIHLVEEAQGERAPAQAFVDKFAKYYTP
                     IIMVIAALVAVVPPLFFGGSWDTWVYQGLAVLVVGCPCALVISTPISIVSAIGNAAKK
                     GVLIKGGVYLEELGAIKAIAFDKTGTLTKGVPVVTDFKVLNDQVEEKELFSIITALEY
                     RSQHPLASAIMKKAEQDNITYSDVRVEDFTSITGRGIQGNIDGTTYYIGSPRLFKELN
                     VSDFSLEFENKVKVLQNQGKTAMIIGTDQTILGVIAVADEVRETSKNVIQKLHQLGIK
                     QTIMLTGDNQGTAEAIGAHVGVSDIQSELMPQDKLDYIKKMKAEHGNVAMIGDGVNDA
                     PALAASTVGIAMGGAGTDTAIETADIALMGDDLSKLPFAVRLSRKTLNIIKANITFAI
                     GIKIIALLLVIPGWLTLWIAILSDMGATILVALNSLRLMRVKDK"
BASE COUNT         1106 a          503 c          716 g          941 t
ORIGIN      
        1 aacaaatcat tggaagtttg acggggtaat tatcaatgga taaacaagtt agaaatacaa
       61 cagaaattgt acgtttggcg aagcagaaat cacaaaagac aagggaaaaa gtagacaaag
      121 cgatttctaa attttcgatt gaaggtaaag ctattaattt taattcaata gcaaaggaag
      181 ctaatgtttc taaatcatgg ctttataagg aacacgatat taggcaaaga atcgaatccc
      241 ttcgtgagcg tcaaataaca tcaaatgtag tctcaaaacc caagaaaagt tctcgttcgg
      301 aggaaatcct tataaaaacc ttaaaaagaa gggttatgga attaaaaaaa gaaaataaaa
      361 aattacagaa ccaaattcaa aaattatatg gagatctgta taataaagag tagttttaat
      421 tattaatctg tagacagatt gtgaaaggat gcacttaaac taacggggca gttatgttta
      481 agatacttta aaatttggtt aaaatctccg aaaggattgg tttctaacat tagaaatcaa
      541 tcctttttaa tattaattat atatcattca aacaatcatt tgactatttg tttatggtgg
      601 gatatattat aatcatcaaa caaacagtca tttgaataag gaggataatt atgacaaaag
      661 atatgtgtga agttacctat attcatgaag ataaggtaaa cagggctaaa aaagaccttg
      721 ctaaacagaa tcctatggat gtagcgaaag tttttaaggc tctatcagat gatacaagag
      781 ttaaaattgc ttatgttttg tctttagagg gagagttatg tgtttgtgat gtagctaata
      841 tcattgaatc ttcaacggct acggcatccc atcatttaag attattgaaa aatttaggta
      901 tagcaaaata ccgtaaagaa ggaaaagtag tctattattc actagatgat gagcatgtta
      961 aacagcttgt agaaaaagct ttcttgtatc aaagggaggt tgctagtatt ggatagttca
     1021 gcaaaaacat taacagaaga taaacaggtt taccgtgtgg agggtttctc gtgtgcgaat
     1081 tgtgctggga agtttgaaaa aaatgtaaaa caactagctg gagttcagga tgcaaaagtg
     1141 aactttggcg cttccaaaat tgatgtatat ggaaatgcat cggttcaaga gcttgaaaaa
     1201 gcaggtgctt tcgagaatct taaggtattt cccgaaaaac tagcgaattc atcgatgcaa
     1261 gcggtcaaag aagacactaa ggctcctaaa gaagagaaaa taccgtttta taaaaaacac
     1321 agcacattgc tgtttgccac attactgatt gcttttggtt acctttctca ctttgtaaat
     1381 ggagaagata accttgtaac ttccatgtta tttgtaggtt cgattgtaat tggcggatat
     1441 tcactattta aagttggttt tcaaaatttg atacgctttg atttcgacat gaaaaccctg
     1501 atgaccgtag cagttattgg agctgccatc attggtgaat gggcagaggc atccattgtt
     1561 gtcgttctct ttgcaatcag tgaagcactt gaacgttttt ctatggatag agcaagacag
     1621 tccattcgtt cattgatgga tattgcccca aaagaagcac ttgttatgcg gaatggtcag
     1681 gaaataatga tccatgtgga cgatattgcc gtgggtgata ttatgatcgt caaaccaggg
     1741 gagaaaattg ccatggatgg gatcattata aatggtgtgt cggctgtcaa ccaggctgct
     1801 ataacaggag aatctgtccc tgttgccaaa acggtagatg atgaagtatt tgcaggtacg
     1861 cttaacgaag agggactact tgaagtaaaa atcaccaaat acgtagagga tacaactatc
     1921 tccaagatta ttcatctggt tgaggaagca caaggggagc gcgctccagc gcaagcattc
     1981 gtagataaat ttgcgaaata ttatacgccg atcattatgg ttattgcggc gctcgttgca
     2041 gtcgttccac ctttattctt tggtggaagt tgggatactt gggtttatca aggattagcg
     2101 gtacttgtag ttggatgtcc gtgtgcatta gttatttcta ctccaatctc gattgtctcg
     2161 gcaattggaa atgcagctaa aaaaggtgtg ttgattaaag gcggtgtcta tctagaggaa
     2221 ttaggagcca ttaaggcaat cgcatttgat aaaacaggaa cactgacaaa aggtgtacca
     2281 gtggtaacag attttaaagt gttaaatgat caagtggaag aaaaagagct gttttccatt
     2341 attacagctt tagaatatcg atcacaacat ccacttgctt cagcaataat gaagaaagca
     2401 gagcaagata atattactta ttccgatgtt agagtggagg acttcacttc tattacaggt
     2461 cggggcattc aagggaatat agatggaaca acctattaca ttggcagtcc aaggcttttt
     2521 aaagaattaa atgtttccga ttttagcctt gagtttgaaa ataaagttaa agttttacaa
     2581 aaccaaggga aaacggccat gattattgga acggaccaaa caatcctcgg cgtgattgct
     2641 gtagcagatg aggtccgcga aacaagtaaa aatgtgattc aaaaacttca tcagttagga
     2701 atcaagcaaa caattatgct gacaggtgat aatcaaggta ccgcagaagc aatcggtgct
     2761 catgtaggcg tttctgatat tcagtccgaa ttgatgccac aggataagtt ggactatatt
     2821 aaaaaaatga aagccgagca tggtaatgta gctatgattg gcgatggcgt caatgatgct
     2881 cctgcacttg ctgcatccac tgttggcatt gcaatgggcg gtgctggaac agatactgcc
     2941 atcgagacag ctgatattgc attaatggga gatgatttaa gtaagcttcc atttgcagta
     3001 agacttagca ggaaaacgct aaatatcatc aaagcgaaca tcacgtttgc catcggaatt
     3061 aagataattg ccttactatt ggttattcca ggctggctaa ccctttggat tgcgattctt
     3121 tccgatatgg gagccactat tttggtagca ttaaatagtt tgcgactgat gagagtgaag
     3181 gataaatagg tactatatgt gtgcaattta cggagaggct gggacataaa tccctaaaaa
     3241 gcagcagtaa gataattttc aattag
//
LOCUS       DAKSQE010000176         3241 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.178, whole genome shotgun sequence.
ACCESSION   DAKSQE010000176 DAKSQE010000000
VERSION     DAKSQE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.178"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(80..1480)
                     /gene="nhaC"
                     /locus_tag="O0V33_002582"
     CDS             complement(80..1480)
                     /gene="nhaC"
                     /locus_tag="O0V33_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCX8977171.1"
                     /translation="MMTRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     AWIAKRVGLTWQDLETGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLSISPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIVGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMLISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNDSMIHQSHISFSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVGEFFIWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
     gene            complement(1575..2657)
                     /locus_tag="O0V33_002583"
     CDS             complement(1575..2657)
                     /locus_tag="O0V33_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HCX8977172.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVESIGLDKLDLEKYLK"
     gene            2905..>3241
                     /locus_tag="O0V33_002584"
     CDS             2905..>3241
                     /locus_tag="O0V33_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HCX8977173.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITFIGVFLIATDIDFIIIIGFIITFI
                     GILSIFAFSI"
BASE COUNT         1091 a          614 c          445 g         1091 t
ORIGIN      
        1 aaaacctttt caaattataa ccgctacaat tataaatgat taaagtgttt caacatatta
       61 tatatattcc acgctcacat taagttaaac gtgaattcga tgacttttta atacctattc
      121 ctgtaaaacc atagataatt gctataattg cgcataaata acatggtact gtccatatga
      181 aaaattctcc aacagagaca tgaagttgat tcgtatagta aatacctgat gttccccatg
      241 gtattaaagg taaaaccatc gtacttgagt cttcaagtgt tcttgataaa acggaacgag
      301 agacttggta tttttcgaac aaatctttca ttaacacacc gaccataata attacaatcg
      361 aagcaacacc tgcagcgaat acaagcgcaa tacaacaaat aacggtaata catattaaac
      421 ttcctacaga atggatgcct ttagaaatag tagttaataa gacttctaaa catcctgctt
      481 tttctacaat acctgcaaat gcatatccgc aaaatatcgt tactaatatt tgggtcatac
      541 tcatcatacc accctgttct aacaagcttt tcacactgaa tgaaatatga gactgatgta
      601 tcattgagtc gttaaaacca ctaaatgttg ctttgaaacc atctgtcatt ttgaaatgat
      661 gattaaaagt ccccactata atggcagaaa agcttgatat tagcatagct ggcactgtag
      721 ccattttaaa tagcaaacaa acaatgatca caattaaggg aacccagacc catatgttaa
      781 tttgataaat ctgtgcaagc tctgataaca atgtttgaat ctgcttatca tttgaatgcc
      841 ctttaaattg aaatccaaca ataaaccata ctaataaacc gatgattgac gcaggtattg
      901 ttgtccacat cattgaatga atgtgtttaa atatattcac tttagtgaca agcgccgcta
      961 aattagttgt atctgataaa ggtgacattt tatcgccaaa tacagcgcct gctataatag
     1021 cacccgcagc cattcctggt gagatactta attgattacc gatagaaata agtgcaatcc
     1081 ctgcagttga tgcagagccc catgctgtac cagttgctac agatgtaaca gcacttataa
     1141 aaaaggctga tattaaaaaa tagcttggat tcaataaatc taagccataa taaatcaagg
     1201 ctggcactgt gccagaaaac atccaactgc ctactataat tcctaccgct agtataatta
     1261 aaattgcagg cattgcagta tttaaacgtt ctgcaatgcc tgtttctaaa tcttgccatg
     1321 ttaagcctac acgttttgca atccatgcgg catatgctga ggcaataatt aataatactt
     1381 gaattggaat atcaaaaaac acaaagcctg ttacaacaac aataaccatt acaatcattg
     1441 tcgaaattga ttctaaaaag gttggttttc ttgtcatcat ctatcgtccc cttcgcttat
     1501 tgatgagaaa cctcttttct tcaatttatt gcttttgcat aatgctatat ccttttctat
     1561 cttcaagcca tcatttattt taaatatttt tctaaatcaa gcttatctaa gccaatggat
     1621 tcaactgtta agccttcctc aaagaagtct ctctctaaaa tggttgatgc aatgacaatt
     1681 actgcatcta tatttggtgt cggtacatca ataacacgac ctaagcttga ccataatact
     1741 aaaccatatg caatatcctc agttaaatat ctgctttcta catgatttgg tcctggaatt
     1801 tgtgaaaata ctgggcttgt attaaacaaa cgatttaatg gttcatcttc tttatcacgt
     1861 tctaaataac cacgttcaat acgtgattct ttagctgttg ataattcaaa acctaatcta
     1921 cggcctaaat ttaaacgttc taattcgatt gcatgaagta atctaactgt atgtttagta
     1981 attccttctt tatataaagc gaactcacca gcataatcaa tacgaccgac attcaataat
     2041 gttggccctg gatgcacttc aggattacca ttttcaagat ttgttttaat taagctttcc
     2101 tcttttacta aatgatcata aatacttgaa actttgtcat aacaatcatt tagacagctt
     2161 ctatcatatg ttgaaaagaa gatacgacgt acatttagag ataaatcaac tgctgcattt
     2221 tcaaaatcga cacgcgtacc atacgtcaac gtattagctt ctgctaattg tggttttgtt
     2281 tcaatatgtc tatcttctaa aacattcata aaacgaattg accccattgc tgcagccatg
     2341 ttgaagaata tcaactgatt atcagttaca tgctctgcca ttacatcagc ataatattct
     2401 atgtatgaag atggaataat cacttgaaca atttcagcat cttttaaaac atattccata
     2461 tcatcactaa tatcagtgaa ttttacgaaa cgttcatcac cttcattatt aaaatcaaat
     2521 ccgccctttt cgattgcgtt ttgaaactta cttatagatt gattacgaca atataattta
     2581 acatcgtggc ctttgctcac catatctact gctgccgtaa ctgcgccatt tcctgatcct
     2641 acaattgcta ttttcataaa caaccctctt tctatctttg gttaaaatta aaaacaaatt
     2701 atacgtaaga caatataaaa atgatgcgaa ttcattacgc atcattataa atgttaagta
     2761 tatataacct taaattaacc tatttaaacg ccgacgtcca ccaattattt ttatttttaa
     2821 taattgttta ataaacatgt ttatttttga ttatattttg ttatagttaa agataatatt
     2881 acaattttca aaggagtgtc taatatgaca actgaaatga acgacgtaac aaatcatcaa
     2941 ccgaatcgca aatccaatga tgagaacatt atggcgatgc ttatctattt attaagttta
     3001 ttcacatcta ttattggtcc actgattata tggttattga aaaaagatga atcgaaacta
     3061 gttgatcgtg ctggaaaaaa ttatcttaac tatacgattt cttatattat ttggtctatc
     3121 gttttagtcg ttatcacttt tataggcgtt ttcctaattg ctaccgatat tgatttcatt
     3181 atcattatag gtttcatcat tacgtttatc ggtattttat ccatattcgc tttctcaatt
     3241 t
//
LOCUS       DAKSQE010000177         3149 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.179, whole genome shotgun sequence.
ACCESSION   DAKSQE010000177 DAKSQE010000000
VERSION     DAKSQE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.179"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            12..845
                     /gene="blaZ"
                     /locus_tag="O0V33_002585"
     CDS             12..845
                     /gene="blaZ"
                     /locus_tag="O0V33_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCX8977174.1"
                     /translation="MFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALDTKSG
                     KEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEKYVGK
                     DITLKALIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIELNYYS
                     PKSKKDTSTPAAFGKTLNKLIANGKLSKENKKFLLDLMLNNKSGDTLIKDGVPKDYKV
                     ADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKSVMKE
                     F"
     gene            complement(1319..1963)
                     /gene="trmB"
                     /locus_tag="O0V33_002586"
     CDS             complement(1319..1963)
                     /gene="trmB"
                     /locus_tag="O0V33_002586"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCX8977175.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGEMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEY
                     FKNGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK"
     gene            complement(1978..2769)
                     /locus_tag="O0V33_002587"
     CDS             complement(1978..2769)
                     /locus_tag="O0V33_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCX8977176.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
BASE COUNT         1108 a          523 c          429 g         1089 t
ORIGIN      
        1 tgaaaaagtt aatattttta attgtaattg ctttagtttt aagtgcatgt aattcaaaca
       61 gttcacatgc caaagagtta aatgatttag aaaaaaaata taatgctcat attggtgttt
      121 atgctttaga tactaaaagt ggtaaggaag taaaatttaa ttcagataag agatttgcct
      181 atgcttcaac ttcaaaagcg ataaatagtg ctattttgtt agaacaagta ccttataata
      241 agttaaataa aaaagtacat attaacaaag atgatatagt tgcttattct cctattttag
      301 aaaaatatgt aggaaaagat atcactttaa aagcacttat tgaggcttca atgacatata
      361 gtgataatac agcaaacaat aaaattataa aagaaatcgg tggaatcaaa aaagttaaac
      421 aacgtctaaa agaactagga gataaagtaa caaatccagt tagatatgag atagaattaa
      481 attactattc accaaagagc aaaaaagata cttcaacacc tgctgctttc ggtaagactt
      541 taaataaact tatcgcaaat ggaaaattaa gcaaagaaaa caaaaaattc ttacttgatt
      601 taatgttaaa taataaaagc ggagatactt taattaaaga cggtgttcca aaagactata
      661 aggttgctga taaaagtggt caagcaataa catatgcttc tagaaatgat gttgcttttg
      721 tttatcctaa gggccaatct gaacctattg ttttagtcat ttttacgaat aaagacaata
      781 aaagtgataa gccaaatgat aagttgataa gtgaaaccgc caagagtgta atgaaggaat
      841 tttaatattc taaatgcata ataaatactg ataacatctt atattttgta ttatattttg
      901 tattatcgtt gacatgtata attttgatat caaaaactga ttttccctct attattttcg
      961 agatttattt tcttaatcct ctttaacaaa ctagaaatat tgtatataca aaaaattata
     1021 aataatagat gaatagttta attataggtg ttcatcaatc gaaaaagcaa cgtatcttat
     1081 ttaaagtgcg ttgctttttt ctcatttata aggttaaata attctcatat atcaagcaaa
     1141 gtgacaacca taaaaggtga agaacctttt tatattcaaa atcaaaaaag cggacatatg
     1201 aaaagtgaaa aaattccatc tgtcgggttt attacagaac caatatgctt tatctaaaga
     1261 accattatag tacgaaaaaa cacttatgat tgtatgtgta cataccctca taagtgctct
     1321 atttttgtga atggaatttc gcttccatac gataaatacg tgacccttta tccgaaaatt
     1381 tcttttcata ttctgttaaa atattactgc catcgtcttc ttgatgtaaa tttagattta
     1441 tttttgtaaa atacattcca aattgagaca tactttctaa actgtaggca aatagtcctc
     1501 tgttatcagt tttaaagtgt aaatctcctt catcatttaa gatttgttga tacaacgcta
     1561 aaaacgtatg atacgttaaa cgtcgttttg catgacgatt ttttggccat ggatctgaaa
     1621 agttcaaata aatacgcgaa acttcgccgt ttttaaaata ttcatttaat tcaatggcat
     1681 cattacaaat aatctttaaa tttgttaaac ccatctcttt aactttatcc aatactttat
     1741 aaacgatact tttctcacgt tccattgaaa tatagttaat atgaggattt tgagcagcta
     1801 atgttgtaat aaactgcccc atacccgaac caatttcaat gtgtatcggt tgcgttttat
     1861 caaaccattc agtcatttcc cctgcatgtt gaccgtccat gtcaaccaat tcaggatgat
     1921 cttttaaata atcttcagcc catggtttgt atcgaactct catattttat tctcctctta
     1981 aataaacatg ttactattca taacttcatt taggaattta agccaagtgt tcatatcctt
     2041 atatcttttt tgctcttcat accattgaac aagacctata gattgaatta ccgtatacca
     2101 tttcatacgt ttatttaaat tcaagctctc ttgaactcca tatgtttcaa gccattcaga
     2161 ccattgttgt tgtggaacat agttgtaaag cagcattccg atatcaattg ccgggtctgc
     2221 aatcattgca ccttcccaat cgactaaaaa tagttcatct cgatcggata ataaccaatt
     2281 attatgattc acatcaccat gtacaacagt gaaaaaacgc gaatctaaac tcggtatatg
     2341 ctcttctaaa taggttaatg attttctcac aatatgatgt gttaaaactt ctcttgataa
     2401 agaggcatta attttattaa gcataatctc aggagtaata ggttccattt ccatacgctt
     2461 taacatactc aataaaggtc tagaattgtg tatcttcttt aataaatgtg caactcttgt
     2521 ttgcttcatt tcatttgaag atagttcacg gccatttttc caatgttgcg ctgtaacaac
     2581 ctcgcctgtt tctatgcgtt tcgtccatac taatttgggc acaatacctt ctgctgataa
     2641 tgccgcaata aatggatttg aatttcgttt taaaaacaac ttttgtccat cttgttcagc
     2701 catatatgct tcaccagatg caccacctgc agaatcaagt gtccacccta attgataaaa
     2761 ctgctccaac tcgtccacct cactttcaat tagaaaatgg ctctagaaat aggtttttca
     2821 agagccatat attctaattt ataacaccat actggtacaa attttatgtc cagataatta
     2881 ttttaaatcc tcaaccaatg cctacattac acgactaaat ttaaatcgta atgtctgtca
     2941 ttgacaccat acattctata gtcacttact tgacataaaa tgttaccgtg tctaaaacta
     3001 catgtttttg taacttttgt aggcgataaa ctctagtttt caaaataatt gctatcccat
     3061 tttcatggtt aacataaatt tatgaactgt aacatttacg tacttagtaa aatatgatgc
     3121 acatcatatt tgtaactcat agaaaattt
//
LOCUS       DAKSQE010000178         3082 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.180, whole genome shotgun sequence.
ACCESSION   DAKSQE010000178 DAKSQE010000000
VERSION     DAKSQE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.180"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            199..708
                     /locus_tag="O0V33_002588"
     CDS             199..708
                     /locus_tag="O0V33_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCX8977177.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDLVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
     gene            720..1790
                     /locus_tag="O0V33_002589"
     CDS             720..1790
                     /locus_tag="O0V33_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCX8977178.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEVLYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTIIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKTIDEEQLTAQILLQELNKMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            1807..2421
                     /locus_tag="O0V33_002590"
     CDS             1807..2421
                     /locus_tag="O0V33_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCX8977179.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFIYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSSIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGVFCIILSTLLLRNKLIN"
     gene            2538..2737
                     /locus_tag="O0V33_002591"
                     /pseudo
     CDS             2538..2737
                     /locus_tag="O0V33_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184337.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2813..2945
                     /locus_tag="O0V33_002592"
                     /pseudo
     CDS             2813..2945
                     /locus_tag="O0V33_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1147 a          382 c          537 g         1016 t
ORIGIN      
        1 atacttttta aatagtatta ttcactagtt ttaacaatca attaattggt atatgatact
       61 tttattggtt atttttatcc catagtgtga taattactat ttttcattca taataaaggt
      121 ttaaagcatg ctaatagtgt gtaagattaa catgtactga aaaacatgtt taacataacg
      181 atataaggag tgacgtacat gatccgtcta ggtaaaatgt cagatttaga tcaaatctta
      241 aatctagtag aagaagcaaa agaattaatg aaagaacacg acaacgagca atgggacgat
      301 cagtaccctc ttttagaaca ttttgaagaa gatattgcta aagattattt gtacgtatta
      361 gaggaaaatg acaaaattta tggctttatt gttgtcgacc aagaccaagc agaatggtat
      421 gatgacattg actggccagt aaatagagaa ggcgcttttg ttattcatcg attaactggt
      481 tcgaaagaat ataaaggagc tgctacagaa ttattcaatt atgttattga tttagttaaa
      541 gcacgtggtg cagaagttat tttaacggac acctttgcgt taaacaaacc tgcacaaggt
      601 ttatttgcca aatttggatt tcataaggtc ggtgaacaat taatggaata tccgccatat
      661 gataaaggtg aaccatttta tgcgtattat aaaaatttaa aagaatagag gtaatattaa
      721 tgacgaaaat cgcatttacc ggagggggaa cagttggaca cgtatcagta aatttaagtt
      781 taattccaac tgcattatca caaggttatg aagtgcttta tattggttct aaaaatggta
      841 ttgaaagaga aatgattgaa tcacaactac cagaaattaa gtattatcct atttcgagtg
      901 gtaaattaag aagatatatt tctttagaaa atgccaaaga tgtatttaaa gtattaaaag
      961 gtattcttga tgctcgtaaa gttttgaaaa aagaaaaacc tgatctatta ttttcaaaag
     1021 gtggatttgt atctgtgcca gttgttattg cagccaaatc attaaatata ccaactatta
     1081 ttcatgaatc tgacttaaca ccaggattag cgaataagat agcacttaaa tttgccaaga
     1141 aaatatatac aacatttgaa gaaacgctaa actacctacc taaagagaaa gctgatttta
     1201 ttggagcaac aattcgagaa gatttaaaaa atggtaatgc acataatggt tatcaattaa
     1261 caggctttaa tgaaaataaa aaagtgttac tcgttatggg tggaagctta ggaagtaaaa
     1321 aattaaatag cattattcgc gaaaacttag atgcattatt acaacaatat caagtgatac
     1381 atttaactgg taaaggatta aaagatgctc aagttaaaaa atcaggatat atacaatatg
     1441 aatttgttaa agaggattta acagatttat tagcaattac ggacacagta ataagtagag
     1501 cagggtcaaa tgcgatatat gagttcttaa cattacgtat accaatgtta ttagtaccat
     1561 taggtttaga tcaatcccga ggcgaccaaa ttgacaatgc aaatcatttt gctgataaag
     1621 gttatgctaa aacgattgat gaagaacaat tgacagcaca aattttatta caagagctaa
     1681 ataaaatgga acaggaaaga actcgaatta tcaataatat gaaatcgtat gaacaaagtt
     1741 atacgaaaga agctttattt gataagatga ttaaagacgc attgaattaa tggggggtaa
     1801 tgctttatga gtcaatggaa acgtatctct ttgctcatcg tttttacatt ggtttttgga
     1861 attatcgcgt ttttccacga atcaagacta gggaaatgga ttgataatga agtttatgaa
     1921 tttatatatt catcagagag ctttattacg acatctatca tgcttggggc tactaaagta
     1981 ggtgaagtct gggcaatgtt atgtatttca ttacttcttg tggcatatct catgttaaag
     2041 cgccacaaaa ttgaagcatt attttttgca ttaacaatgg cattatctgg aattttgaat
     2101 ccagcattaa aaaatatatt cgatagagaa agacctacat tgctgcgttt aattgatata
     2161 acaggattta gttttcctag cggtcatgct atgggatcaa ctgcatattt tggaagtagt
     2221 atctatttat taaatcgatt aaatcaaggt aattcaaaag gtattcttat agggttatgt
     2281 gcagctatga ttttattgat ttccatatca cgtgtatatc taggtgtaca ttatccaaca
     2341 gatattattg ccggcattat tggtggcgta ttttgcatta ttttatcaac gttattactt
     2401 agaaataaat taatcaatta aatagtaaaa aaacaaaagc agtaaaccta aagtgtcgta
     2461 agggtttact gcttttataa aacgttgtta taacgtatat tgtcttttac gggcatataa
     2521 aaggggaata tttgaaaatg accaatccaa caagaacgaa acgttgtggg gggatgttct
     2581 atgtggtatt gataatcatt ttcaactact attatacatt agtgagaatc attgtcaatt
     2641 agaaactaaa actttttttg aatatttttt aagaatagta aataaaacgc atgattacgc
     2701 tattttagaa aataaaaaaa tttgtatttc tcagtagaat tagaatattt aaaagtgatg
     2761 aggcttaaaa attatattgt tacatacttc ttttgaattc atacattaag aaatgttact
     2821 tgcaagttca aacaggcaca ttgaaatgat gtgtgataaa tgtttaaact acggtcattt
     2881 tgtgaaaata aagataataa ctattcattt tacattaata aaagtcagta tacgacaaca
     2941 attgatattg cggtaagacc ttgtgttaca gtattctata tttaagtact gcaatcagaa
     3001 ttaacagaat tccattaact gattattaaa tatttgagtt aataaataat taatgattgt
     3061 agcttgaaaa atttaaaaca tg
//
LOCUS       DAKSQE010000179         3058 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.181, whole genome shotgun sequence.
ACCESSION   DAKSQE010000179 DAKSQE010000000
VERSION     DAKSQE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.181"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            64..513
                     /locus_tag="O0V33_002593"
     CDS             64..513
                     /locus_tag="O0V33_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-inhibiting protein CHIPS"
                     /protein_id="HCX8977180.1"
                     /translation="MKKKLATTVLALSFLTAGISTHHHSAKAFTFEPFPTNEEIESNK
                     KMLEKEKAYKESFKNSGLPTTLGKLDERLRNYLKKGTKNSAQFEKMVILTENKGYYTV
                     YLNTPLAEDRKNVELLGKMYKTYFFKKGESKSSYVINGPGKTNEYAY"
     gene            complement(608..943)
                     /locus_tag="O0V33_002594"
                     /pseudo
     CDS             complement(608..943)
                     /locus_tag="O0V33_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
     gene            complement(1593..2084)
                     /gene="sak"
                     /locus_tag="O0V33_002595"
     CDS             complement(1593..2084)
                     /gene="sak"
                     /locus_tag="O0V33_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylokinase"
                     /protein_id="HCX8977181.1"
                     /translation="MLKRGLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASY
                     FEPTGPYLMVNVTGVDGKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKE
                     FRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVII
                     EKK"
     gene            complement(2275..3030)
                     /locus_tag="O0V33_002596"
     CDS             complement(2275..3030)
                     /locus_tag="O0V33_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCX8977182.1"
                     /translation="MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIR
                     MWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQ
                     FLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKVKPPAQKAVGKS
                     ASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTL
                     IYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF"
BASE COUNT         1056 a          547 c          425 g         1030 t
ORIGIN      
        1 ttaggaattt cgcaaaacta actataaatt taaatataga atttaaggag aattaacatc
       61 attatgaaaa agaaattagc aacaacagtt ttagcattaa gttttttaac ggcaggaatc
      121 agtacacacc atcattcagc gaaagctttt acttttgaac cgtttcctac aaatgaagaa
      181 atagaatcaa ataagaaaat gttagagaaa gaaaaagctt ataaagaatc atttaaaaat
      241 agtggtcttc ctacaacgct aggaaaatta gatgaacgtt tgagaaatta tttaaagaaa
      301 ggcacaaaaa attctgctca atttgaaaaa atggttattt taactgaaaa taaaggttac
      361 tatacagtat atctgaatac accacttgct gaagatagaa aaaatgttga gttactaggt
      421 aaaatgtata aaacatactt ctttaaaaaa ggagagtcta aatcatctta tgtaattaat
      481 ggtcctggaa aaactaatga atatgcatac taatagtagt tacataaatt aaaaggtaga
      541 tatttctttt ttatataaag gtttggcaga catttcataa cttgccaaac ctttatatat
      601 ctaattatca aactgcacta aacttaccaa aactgcttat tctattacct gccttgtcta
      661 cctctcctgt cgctatataa cgacgttgtc cactattagc aatataagta atccatctat
      721 agccattaat gcaatatgcg ccgtcatatt tgatagttgc gttattgggt aatacacctg
      781 taattcttga attagttgaa tagccatccc tcacgttatt acctttaaca ttggcaactg
      841 tgtaataacc agtctctttt ttatacggta cattgttttt atcgagtgta taacctgctg
      901 gcactggtga attcttttca tttttagctg gtgttttaac attactgata cctgatacac
      961 acttccaata aaaataacca caccatttaa gatgcggtgt agcgactgta atatttctat
     1021 gttgttgaga tatatgtatc gagtgaaggg caaaaaggat atcaattgca ggataagtgt
     1081 taggttacta ggccacttaa caggctatat agttcactcc tactatatac agttaattat
     1141 aacataaaaa gcaccccgta aactattata cgggaatgct aaagtcatat atactacgag
     1201 ggagtagtat gaaaactatg ctctctatca taagaaaaaa cacccagtga catgcttggg
     1261 tgaacaagga tagatgtaaa tagttgatgc atgtgtaaca catcataaca aaaaactagc
     1321 ccgaaagcta gctataacat acaatctaaa aagacgtccc ttgaatacgt ctagaaagat
     1381 tataacataa aaaaataggc aagtaccgaa gtacctgcct aaaaaaggat tatccacttt
     1441 ttcatcctaa ctgatttctc cccataagtc acctaatatc tgattaggtg gggaagaacc
     1501 attcgtgcat gaatgagaat ttgatgaaag ataattttca ctacacatat tcaatcaaga
     1561 cattgctttc tataataaac aactattttg ttttatttct tttctataat aacctttgta
     1621 attaagttga atccagggtt tttaatatgc tctgataaat ctgggacaac aaaacctttt
     1681 tctgttatag ggaaagactt cgtttcttct tttttcttat tcttatcata ataagtgact
     1741 tcgatctttg cgcttggatc taattcaact actctaaact ctttatatgc tgtcgcatct
     1801 aatgcccatt cgacatagta ttcaattttt tcttttgtaa gtgtagtccc aggtttaata
     1861 ggaaactcga cataatgagg ggatagcaat tcatttcctt taccatcaac tccagtcaca
     1921 tttaccatca aatacgggcc tgttggttca aaataactcg cgtcatcgcc ttttttatat
     1981 tttcctttgt cgaatgaact tgatgcactt acctcattag taattgaaga aaatgagaat
     2041 aataacaata aaacagttaa aaataataaa cctcttttga gcatggcgct tcctccaaat
     2101 ataatatatt tgtttttatt tatctctttt tatagctaac acccttattt aagatgataa
     2161 atatcaacaa cttcatttta tattgagaaa atattaaaaa tcaataaaat attaaaagaa
     2221 actaaaatta cattaaaaaa taattaacaa atatttaaca ttttaaccta agaattaaaa
     2281 cacttctttc acaatcaatc tctcatgcca tatccactca ttatgattgt tccaataaat
     2341 gcgacaccaa ccatctataa tttcaaacac atatattaat gttccaggcg cgtatacagc
     2401 ctgtccaaca tcgaatctat agttagtacg attatcaccg tatctagtgg ctgaagtagc
     2461 acctaagcca tcgattttcg cattaaaata agcacctttt gaccatttaa ggttataagg
     2521 cgctttactt ccaactgtta ttttacttgc agatttaccg actgcttttt gagcaggtgg
     2581 tttaacttta tttgtgatct tattcattaa gccctcactt ttatacttag gtctaataaa
     2641 gtgagtacag ccgtaataat tatcccaacg taactttgca ggcgtatttg cgttaccgtc
     2701 atagttctgt tccaaaatta aaaattgatt tgtattacca ccattaaaca ctaaaccaat
     2761 atgaccgtat tgtttatata ttcctttggt aaatacagcc acatcaccta tttgtggaac
     2821 aaacgatggt gtgttttcat atactgttgc catgttttta aaatcgttat tgattgcatc
     2881 ttttgcattt ccccacattc taatttctaa caaccaataa atgtaatcaa ctgctaaatc
     2941 tgcacattgg taaccatacc aaccgtcaaa atcaatatat ctaccttgat accaacgtaa
     3001 ccttgctctt gcttcactgt atgttttcat tattttacct cctagtattt tcttcttg
//
LOCUS       DAKSQE010000180         2968 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.182, whole genome shotgun sequence.
ACCESSION   DAKSQE010000180 DAKSQE010000000
VERSION     DAKSQE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.182"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            154..726
                     /locus_tag="O0V33_002597"
     CDS             154..726
                     /locus_tag="O0V33_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HCX8977183.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDSYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYITLSHFGINIDESEIYNYLFEDMSDY
                     YPSYFKKYNQSEVKHYLHDIQKSYLKSRGN"
     gene            729..1454
                     /locus_tag="O0V33_002598"
     CDS             729..1454
                     /locus_tag="O0V33_002598"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HCX8977184.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGMKALDVCCGTGDWTIALSKAVGSTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEADFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            1456..2415
                     /locus_tag="O0V33_002599"
     CDS             1456..2415
                     /locus_tag="O0V33_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCX8977185.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQLSTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDS
                     IDEAKEVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            2507..2956
                     /gene="ndk"
                     /locus_tag="O0V33_002600"
     CDS             2507..2956
                     /gene="ndk"
                     /locus_tag="O0V33_002600"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCX8977186.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE"
BASE COUNT         1115 a          396 c          555 g          902 t
ORIGIN      
        1 gtaagggtta ttatgagaat tacaaaagct acataaatta ccgttagttt aaattgaaat
       61 ttaaaaatga taagtaatct taatgcttca aaagatttta atttcttaca tataatgtta
      121 atatgaatag tgataatatc gtgaggtgta gacatggaaa caactgttag caaattggaa
      181 agacaaatag aagaaagatt aaagggtgta tcagaatatg aatccattaa tattaatcat
      241 cggttaggta agttgcttga ttcatatgat attccagatg ttgccaaagt agcatgcctc
      301 acaattgaca catcaatgag acaccttgat gacattacat ataatcattt gtctaaacat
      361 tccattttga ttggagattt aattagtgca catttttata cgctactagc agaaatcaat
      421 gatttatcat ttcaaaatga aattagtaaa gcaattgttg aaattaatga attgaaatct
      481 tcattacatc atcaagcttt aaatgattat gaaatttctc aagcaatagt aaaaattgaa
      541 acacttttcc catatattac gttatctcat ttcggtatca atattgatga gtcggaaata
      601 tataattatt tatttgaaga tatgtctgat tactatccat catacttcaa gaaatataat
      661 caaagtgaag taaagcatta cttacacgat atccaaaaaa gttatttaaa aagtagaggt
      721 aattaagcat ggctgacaat aaagcaaata aagagcaagt acatcgcgtt tttcaaaata
      781 tttcaaaaaa atatgacaga ttaaataata ttattagttt tgagcagcat aaagtatgga
      841 gaaaacgtgt catgaaagac atgggtgtta gaaaaggtat gaaagcatta gatgtttgtt
      901 gtggtactgg tgattggaca atagcattaa gcaaagctgt aggatcaaca ggagaagtta
      961 ctggcattga ctttagtgag aatatgttag aagtaggaaa agaaaaaact gcttcaatgg
     1021 aaaatgttaa acttgttcat ggtgatgcaa tggaattgcc gtttgaagac aattcttttg
     1081 attatgtaac aattgggttt ggattaagaa atgtgccaga ctatttagtc gcgttaaaag
     1141 aaatgaatag agtacttaaa ccaggtggta tggtggtatg tcttgaaacg agccaaccaa
     1201 ctttgccagt ctttaaacaa atgtatgcat tatactttaa atttgttatg cctatttttg
     1261 gaaaattatt tgcaaaatca aaagaagaat atgaatggtt acagcaatct acgtttaatt
     1321 ttcctggaaa agaagagttg aagcgcatgt ttgaagaagc ggatttcata aatgtaagag
     1381 tacgtagttt tacagggggc gttgctgcaa tgcaccttgg ctataaagaa aaagataata
     1441 ccaaaggtga ttaacgtggc aaagttaaac atgaacaatg aaattaagaa agtggaacaa
     1501 cgacttgaaa aggcaataaa aagtaaagat tctgtattag aacaggcatc attacactta
     1561 ttgtcttctg gtggtaaaag agtacgccca gcatttgtaa ttctgagtag ccaatttggc
     1621 aaagatgaac aaacgtctga acaaacgtat caagttgcag tcgcattaga gttaattcat
     1681 atggcaacac ttgttcatga tgacgttatt gataaaagcg acaagcgtcg aggcaagtta
     1741 accatatcaa agaaatggga tcagacaact gctattttaa ctgggaattt tttattggca
     1801 ttaggacttg aacacttaat ggcagttaaa gataatcgtg tacatcaatt aatatctgaa
     1861 tctatcgttg atgtttgtag aggggaactt ttccaatttc aagaccaatt taacagtcaa
     1921 cagacaatta tcaattattt acgacgtatc aatcgcaaaa cagcactgtt aattcaatta
     1981 tcaactgaag ttggtgcaat tacatctcaa tctgataaag aaactgtacg aaaattgaaa
     2041 atgattggtc attatatagg tatgagcttc caaatcattg atgatgtatt agacttcaca
     2101 agtaccgaaa agaaattagg taagccggtc ggaagtgatt tgcttaatgg tcatattacg
     2161 ttaccgattt tattagaaat gcgtaaaaat ccagacttca aattgaaaat cgaacaatta
     2221 cgtcgagata gtgaacgcaa agaatttgaa gaatgtatcc aaatcattag aaaatctgac
     2281 agcatcgatg aggctaagga agtaagttcg aagtatttaa gtaaagcttt gaatttgatt
     2341 tctgagttac cagatggaca tccgaaatca ctacttttaa gtttgacgaa aaaaatgggg
     2401 tcaagaaaca catagtattt atgaaaagta ttgaaagcgc tttaccaacc tgttaatata
     2461 taatagtaat atacataaac ctattaaaca cagggggata cataaagtgg aacgtacatt
     2521 tttaatgatt aaaccagacg cagtacaaag aaatctaatt ggtgaagtaa tttcaagaat
     2581 tgaacgaaaa ggactaaaac ttgtcggtgg taaattaatg caagtaccaa tggaacttgc
     2641 tgaaacacat tatggtgaac accaaggtaa accattttat aatgatttaa tttcatttat
     2701 tacatcagca ccagtgttcg caatggtagt tgaaggtgaa gatgcagtta atgtatctag
     2761 acatattatt ggcagcacca atccttcaga agcttcacca ggatcaatta gaggtgattt
     2821 aggtttaact gttggtagaa atatcattca cggttcagat tcattagagt ctgctgaacg
     2881 tgaaattaat ttatggttta atgaaaatga aattactagc tatgcttcac cacgtgatgc
     2941 atggttatat gaataaaata taagctat
//
LOCUS       DAKSQE010000181         2966 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.183, whole genome shotgun sequence.
ACCESSION   DAKSQE010000181 DAKSQE010000000
VERSION     DAKSQE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.183"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            234..959
                     /locus_tag="O0V33_002601"
     CDS             234..959
                     /locus_tag="O0V33_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL14"
                     /protein_id="HCX8977187.1"
                     /translation="MKKNITNKIVLSTALLLLGTTSTQPPKAPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHLSFSGKWLWQNPNGTIHATLQTWVWYTHIQVFGPESWGNINQL
                     RDKYVDIFGTKDEDTIEGYWTYDETFTGGVTPAATSSDKPYKLFLKYKDKQQTMIGGL
                     EFYQGNKPVITLKELDFRVRQTLIKNKKLYNGEFNKGQIRITGGGNHYTIDLSKKLKL
                     TDTNRYVKNPRHAEIEVILEKSH"
     gene            1069..1794
                     /locus_tag="O0V33_002602"
     CDS             1069..1794
                     /locus_tag="O0V33_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL13"
                     /protein_id="HCX8977188.1"
                     /translation="MKNNFTKKIILSIALTMIGTATSQSPHSPISMSSEAKAYNISES
                     ETNVNELTKYYTQRHLTFSNKWLWQKDNGTIHATLLQLSWFSHIQVFGPESWGNINQL
                     RNKYVDIFALKDYETWRTYMLAQETFTGGVTPVAKPSDKHYKLNVTYKDKAGTFIGEY
                     QFYTGNKPVLTLKEVDFRIRQTLIKNKKLYNGDYNKGHIKITGGSNNYTIDLSKRLKS
                     TDANRYVKNPQTAHIEILLEKSS"
     gene            1903..2619
                     /locus_tag="O0V33_002603"
     CDS             1903..2619
                     /locus_tag="O0V33_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HCX8977189.1"
                     /translation="MRKCIMKKLILMTTLLLLGTTATQTSNSPLNVSTDAKAYHIGQD
                     ETNINELIKYYTQLPLTFSNRWLYQYDDGNIYVEFKRYSWSAHIRLWGAESWGNVNQL
                     RDRYVDVFGLKDEDTSQLWWVYRDTFTGGVTPAASPSDKPYSLFVQYKDKLQTIIGAH
                     KMYQGNKPILTLKEIDFRARETLIKNKILYHENRNKGKLKITGGGNDFTIDLSKRLHS
                     DLANVYVKNPQKITVEVLID"
BASE COUNT         1111 a          480 c          464 g          911 t
ORIGIN      
        1 caataaatag ctaatttaac gcaattaata tcataattag catcagtttt aatttattag
       61 cactcttttc attttataat tggcaatgtt ccaatgctta aaaatgaaaa attttactta
      121 aatcacacgt tcaactgttt atgtttattt aacatagtgt tagcttttat ttaattccga
      181 atcggtgcaa taccttatat actatgggta attcacgcaa aggagattat catatgaaaa
      241 agaacatcac gaacaaaatt gttttatcaa cagcattgtt acttttagga actacatcaa
      301 cacaaccgcc taaagcacca atcagttttt catctgaagc aaaagcatac aatataagtg
      361 aaaacgaaac gaatatcaat gaattaatca aatattacac gcagccacac ctttcatttt
      421 ctggaaaatg gctatggcaa aaccctaacg gtaccataca tgcaacatta caaacgtggg
      481 tttggtacac gcatattcaa gtatttggtc ctgaaagttg gggcaacatt aatcaattaa
      541 gagataaata tgtagatatt tttggtacca aggatgagga tactatagaa ggctattgga
      601 cttatgatga aacctttact ggaggtgtaa caccagcagc tacttcatct gataagcctt
      661 ataaattatt tttaaaatat aaggataaac aacaaactat gattggtgga cttgagtttt
      721 atcaaggtaa taaaccagta ataactttaa aagaactaga tttccgcgta cgtcaaactt
      781 taattaagaa caaaaagcta tataatggag aatttaataa aggtcaaatt aggattacag
      841 gtggtggtaa tcattatacg attgatttga gtaaaaagtt aaaactaact gacacaaacc
      901 gttatgttaa aaatcctcga catgcagaaa ttgaagtcat tcttgaaaaa tctcattagt
      961 atcattcatt gagtgaatac aaatagactg aatgatttaa ttgatttctc tctttttata
     1021 aacttattat tcctcgcata taacattcct cgtaaaggag attttctaat gaaaaacaac
     1081 ttcacaaaaa aaattatttt atcaatagca ttgactatga taggcacagc tacaagtcaa
     1141 tcccctcatt caccgattag tatgtcatct gaagcaaaag catacaatat aagtgaaagc
     1201 gaaacgaatg taaatgaatt gactaaatat tacacacaac gccatttaac attttcaaat
     1261 aaatggctat ggcaaaaaga taatggtaca atccatgcaa cattgttaca gttatcatgg
     1321 tttagtcata ttcaagtatt cggccctgaa agttggggta atattaatca attaagaaat
     1381 aaatatgtag atatttttgc tttaaaagat tatgaaacct ggcgcactta tatgttagcc
     1441 caagaaacat ttactggcgg tgttacacca gtagcaaaac ctagcgataa acattataaa
     1501 ttgaatgtaa catataaaga taaagcagga acatttattg gcgaatatca attttataca
     1561 ggtaataaac cagttttaac tttaaaggaa gttgatttcc gaatccgcca aacactaatt
     1621 aaaaacaaaa aattatataa tggtgattat aataaaggcc acattaagat tacaggtggc
     1681 agcaacaact acacaataga tttaagcaaa agattaaaat caactgatgc aaatagatat
     1741 gttaaaaacc ctcaaactgc acacatcgaa atcctccttg aaaaatctag ctaacatcga
     1801 aatcgaagta aacagaaata agcacaatgt ttacattgac gccgttcatt tttaaatttg
     1861 ttaattctcg tattgatgat tacgcagaaa ggagaagttc gaatgagaaa gtgcattatg
     1921 aaaaagttaa ttttaatgac aacattgtta ttattaggaa caacagctac acaaacctct
     1981 aattcgccat taaatgtttc tacagacgct aaagcttatc atattggcca agacgaaacg
     2041 aacatcaatg agttaattaa atattacaca caactgcctc tcacattttc aaatagatgg
     2101 ttataccaat atgatgacgg caacatttat gttgaattta agcgttattc ttggtcagcg
     2161 catatacgat tatggggtgc tgaaagttgg ggcaacgtta atcaattaag agaccgctat
     2221 gtagacgtgt ttggtttaaa agacgaagac actagccagt tatggtgggt atatcgtgat
     2281 acatttacag gtggtgtaac acctgctgca tcaccttctg ataaaccata tagtcttttt
     2341 gtccaataca aagataaact acaaacaatt attggtgcac ataagatgta ccaaggtaat
     2401 aaaccaatat taacattgaa agaaattgat tttcgagcac gtgaaacact aattaaaaat
     2461 aaaatattat atcatgaaaa tcgcaataaa ggtaaactta aaatcaccgg tggcggcaat
     2521 gactttacaa ttgatttgag taaaagatta cattctgatc ttgcaaatgt ttatgttaaa
     2581 aatcctcaaa aaataacagt cgaagtcctc attgattaaa taaggtcgta tgatttatat
     2641 cgtaaatact aaggataaac aatatttgag taatactaca aaatgtctca atagtactta
     2701 cacaatcatt aatttagtac acagaatatt aaatgcgatt tggagtctaa tagaaatgcc
     2761 aagtcattct ctgggtttgg gcccgcccca acttgcattg cttgtagaaa ttggtaaccc
     2821 aatttctcta tgttggggcc ccaccccaac ttacattgtc tgtagaattt ctattcgaaa
     2881 ttctttttgt tggggcccct gtctctaatt taaagtcttg ttataatcgc atttttgttt
     2941 ggtcaatgtc tgtctatata atattg
//
LOCUS       DAKSQE010000182         2932 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.184, whole genome shotgun sequence.
ACCESSION   DAKSQE010000182 DAKSQE010000000
VERSION     DAKSQE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.184"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(51..1367)
                     /locus_tag="O0V33_002604"
     CDS             complement(51..1367)
                     /locus_tag="O0V33_002604"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HCX8977190.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRKYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVSVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEIDAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYVIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(1419..2243)
                     /locus_tag="O0V33_002605"
     CDS             complement(1419..2243)
                     /locus_tag="O0V33_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCX8977191.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIERNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            2357..2665
                     /locus_tag="O0V33_002606"
     CDS             2357..2665
                     /locus_tag="O0V33_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCX8977192.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
BASE COUNT          896 a          498 c          487 g         1051 t
ORIGIN      
        1 acaaaattct aacctagaca attaagatta gattgaatcg catgattcat ttatttagct
       61 ttgtaaccaa tcatattgat taaatcttta gggtggctat atggtggtgc ataagccact
      121 tcaaactcag ttaactcatc tacagttagc tggttcatca ttgccatcga tagtacatca
      181 atacgtttat ctgcgccttc ttttcctact gcagctgctc ttaaaatctg acggtttgaa
      241 gtgtcataat atactcttaa gtgtaaaggg gaatttcctg ggtaataatt cgcgtgtgca
      301 ccttgagtga cttccaccat tttatagtca aattgcttta gttcgtttgg tttaacgccg
      361 acactcgcaa atgtataatc aaagaacttc acaatattgt tgcctaagaa gcctttgaat
      421 tcaatagtgt catttccagc aatttgttcg gcaacaatac ttgctgcacg gtgagcgccc
      481 caagctaaag gaacactagc cggtagatcg acatgtcgat aatgtgatgt tgcaatatcg
      541 cctattacat aaatgtttgg aacatttgtt tcaaatttat cgtttaccgg tatgaaacct
      601 tttcgatcaa gtttgatatt tgaactttcg ataaattttg aattggggtg agtaccgaca
      661 ccttcaataa tcatatcgta atgttcaact tttcctgatt taaatgtaat ttcatttcca
      721 ttgatagcat caatttcctc atttaaacgg tatggaatct cccgcttatc taattcatca
      781 agtataggtt gattcatatc ggcatccatt aatttattta tcttatcaga tcgatgaatt
      841 aaagtagggt gtaaaccacg ttcataaaga ttttcaagaa cttctaatga aacataccct
      901 gcacctacaa ccaatacttt atcaacttga tttgctttga tgaattgatc gatagcatca
      961 gtgtcttcta aatttctaag tgtaaatgta atatcacttt caaagccaag gctatttgca
     1021 cttgcaccag ggcttaaaat gagtttatcg tatgattctt caaattgttc gttggtcttt
     1081 ctatttaata cagatacagt ttgtctttca tcattgattg caataacttc atgataagtt
     1141 tttactgtaa tttgttttct atcataaaat ttttcaggtg tatacgctaa agcatatttt
     1201 ctatcttcaa caacttcgcc aatgacataa ggcaatgcac aattagcaaa gctcatatca
     1261 cgatcttttt caaaaataat aatgtcactt tctttatcta aacgtcgaat ttggctggca
     1321 catgttgctc cgccagcgac tgctccgact acgactattt tgggcatcgt aacttacctc
     1381 ctattgtttt aaaatattat aaaatgatta gttataaatt aacagtaata tctaatattt
     1441 aaattaaaga aatcatttaa atatcgacca attccatctt cattattgtt gaatgtaata
     1501 ttgttcgcta catctttaag ttcttgcaaa ccattttcca tagcaacacc gtgacgcgcg
     1561 tactcaatca tttctatatc attatcttca tcaccgaatg caataatatt atttcgttca
     1621 atatttaaaa attgtctaac ttgttcaatg cctcttgctt tattaattcc aagttttaca
     1681 atttcaatga cagggaatgg tgcgccccag cgtcgatgct caatatgatc ggcataaaaa
     1741 tgagtaagca tatttttgat ttcaggtatt ttactttctt cggcttcaat taaaattgag
     1801 gtaggggatt ctttcaagtg gacaagtaaa ttaccagttt gaattcttgg attacccatt
     1861 gaaaaacctt caaataatct tggatcatga ttattaatga aaacataatc tttcacttct
     1921 gcaataatat tcgatacttg atattgttgt aatccttgaa taatattttg tgcgatgcct
     1981 aaatctaaaa tttcatggca agttttgaag tttttatctt tagggtgatg tacgtaagcg
     2041 ccattaaaat taacaattgg tgtcgttaaa tttaattcat gataatacat ttgacttgca
     2101 cgataaggtc tgccagtcgc aatcataatt tggtgtccac gttgttgtaa ttcatttaat
     2161 acttgtttag tatatgatga aatttctttg ttatcgttta ataatgttcc gtctaagtct
     2221 agacatatta aatgtggttg catatctctc tcctcataag ttgcttagtt catatagata
     2281 catcatagca ttttattggc gttttgtcgg atatttgata tattatgaat aatatgacat
     2341 taaagaggtg atatggatgg aagaggcatt gaaagatagt atcttaggtg cattagaaat
     2401 ggtaattgac cctgaattag gaattgatat cgttaattta ggtttagtat acaaagtgaa
     2461 tgttgatgat gaaggcgtat gtacagttga tatgacttta acatcaatgg gatgtccaat
     2521 gggacctcaa attattgatc aagttaaaac agtattagca gagattcctg aaattcagga
     2581 tactgaagtg aatatcgtat ggagtccacc ttggacaaaa gatatgatgt cacgttacgc
     2641 taagattgca cttggtgtga gctaacataa acttgcatta cttgtagaaa ttggaaatcc
     2701 aattggggct ccgctcccaa cttgcattgc ttgtagaatt tcttttcgaa attctctgtg
     2761 ttggggcccc gcagtcaata taacattgta gagtataatt cattgtgaat aagctccctt
     2821 caaagtagac attgaaggga gccttattta cgttctaggt tctgacttta ttttgaaaat
     2881 ttatatctaa cttgtttcag aaaatatgaa ttaagacagt gacttgcact tc
//
LOCUS       DAKSQE010000183         2923 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.185, whole genome shotgun sequence.
ACCESSION   DAKSQE010000183 DAKSQE010000000
VERSION     DAKSQE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.185"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..786
                     /locus_tag="O0V33_002607"
     CDS             <1..786
                     /locus_tag="O0V33_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCX8977193.1"
                     /translation="NLFFAMCNKLKEQDTNTLRLSFDELKKLSNYSPETRNINRFAND
                     LDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIHKREQYLEISTSSNLKHILNSITNNF
                     TKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLKIHIDDFKNRLDIPKSYRMTDINKNV
                     FKPIIIELGSIFNNLTINKIKAKKGRKIEWIEFTFDAEKRIHNKRQPQMSKIDKSRQY
                     VRREKTPKWLEERSYEKQPQKDYDPQLEKEREDFLKQLELNWE"
     gene            873..1214
                     /locus_tag="O0V33_002608"
     CDS             873..1214
                     /locus_tag="O0V33_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCX8977194.1"
                     /translation="MKLAEAIKEQRELKRWSQEELANILKVSRQSVSKWESAKNYPSL
                     DILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRAPSIVDFLYDFWWLFFPVAG
                     FLVWAIHSFMG"
     gene            complement(1429..1524)
                     /locus_tag="O0V33_002609"
     CDS             complement(1429..1524)
                     /locus_tag="O0V33_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCX8977195.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGYWLHKRDKK"
     gene            complement(2074..2816)
                     /locus_tag="O0V33_002610"
                     /pseudo
     CDS             complement(2074..2816)
                     /locus_tag="O0V33_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
BASE COUNT         1017 a          426 c          437 g         1043 t
ORIGIN      
        1 aatttatttt ttgcgatgtg taataaatta aaagagcaag atactaatac attacgtttg
       61 tcttttgatg aattaaaaaa attaagtaat tatagtccag aaacacgtaa tattaataga
      121 tttgctaatg atttagataa tgtgtataaa aagatgttga atttaaccat tagatatgaa
      181 gatgatgatg taatagaaag attcgtttta ttcaatcatt atagaatcca taagcgagaa
      241 caatatctag aaatatcaac ttcttcaaat ttaaaacaca tattgaattc aattactaat
      301 aattttacaa aatttgaatt aaaagaaatg actaggctta aatctacata ttctaaaaat
      361 atgtttagat tacttaaaca atacaaacat acaggctatt taaaaattca catcgatgat
      421 tttaaaaatc gtttagatat acctaaatct tatcgtatga ctgatattaa caaaaatgtt
      481 tttaaaccta ttattattga attaggttct atatttaata atcttactat caataaaatc
      541 aaagcgaaaa aagggcgtaa aattgaatgg atagagttca catttgacgc tgagaaacgc
      601 attcataaca aacgacaacc tcaaatgtcc aagatagata aatcaagaca atatgtgagg
      661 cgtgaaaaga cgcctaaatg gttagaagaa cggtcatatg aaaagcaacc tcaaaaagat
      721 tacgacccac aattagagaa agaacgagag gattttttaa aacaacttga actgaattgg
      781 gaataacgca acttttcgtt gcgagttggt taactaaaag tatcgtgact tttcaattat
      841 tttaattata gtttgagtta aggagtgatt ttatgaaatt agctgaagct ataaaagaac
      901 agagagaatt aaaaagatgg tctcaagagg aattagccaa tatattaaag gtttccagac
      961 aaagtgtttc taaatgggaa agtgccaaaa actatccttc cttagatatt ttgattgcta
     1021 tgagcgattt atttggaatc tctttagagc atttaattaa aggagatgca cgttttaaaa
     1081 aagttatttt agagggaaat tatagagaga gtaacagggc accttccata gttgattttc
     1141 tttatgattt ttggtggctt ttctttcctg ttgccggatt tttagtttgg gccattcatt
     1201 cgttcatggg ttgattttaa aatcaaaatt taatgcacat caggcaatat gaaaaaggaa
     1261 agtattttta cgacaccttg aaatgtattg ataagaatag aagagtttac aaaaggcaat
     1321 atgaaatata tactaaaagt aacaagaatt tcgtttgtaa acgcaccaaa ccccgaacta
     1381 ttcgccgtag ttcggggttt tttggtgaat tggctttaat cgtctatatt attttttatc
     1441 acgtttatgg agccaataac caaataacgt aacgagacaa ccactgattg ccgttgtcat
     1501 gatgtgaaca agaatttcat tcataaatcg cacctcctct ctacgccgaa ttgacgtctg
     1561 agagatagac gaccttcaca ttataacaaa tctcttcttc catgaaataa catgaatatt
     1621 aataaatgtt gttatatatt aaagataaat aaatataagg gtgggattga taatgtttgg
     1681 cgatcaaaga taagaagcta caaaatatgt cattaaagaa ggttatcaag atatttattt
     1741 cctcaacaaa aacggggaat ggtattattt cgaagtaaga agtgcctggc gtggcaaaca
     1801 tattattaga gttaaagatg gtttgttagg ttggagaaaa gaaatagtaa ctgaataaag
     1861 atacaaaata cttgccaatg caaaagaatg aaaacgcgtc aaatcaacga tgtataaaag
     1921 atgcataaaa gataatgaaa gtaaaaagcc ataaatgcct atatgacggc atttatggct
     1981 ttttgtttgg ttaacataat cgatgattac cagaagttct cactatataa aagacactaa
     2041 aattttataa cttttagtgc ctaatcaatt attttagcta ccaaacaaac gactccaaaa
     2101 ccccttttta ggttccgttt cttcactctt ttcttctata tcactatcat tgtcatttag
     2161 attagctgga acgtcttctc tttttgcttc ttcaccttgt gtactttcgt cgctttgact
     2221 tccagatata tccttatatt gaatctctgt ttctttggtt tcttcctgat ctctgttaat
     2281 atttacagga ttagtagtat atgttttttc ttgtgcatca acgttttgtc tagtgttagt
     2341 agatgtatca aagggataat taaggtgttt ttctttttct aattgatttt ctaatttttg
     2401 aatcttttta ttactctcta atgccagaac ttgctgattt tcaagcaatt ttgatctttc
     2461 ttgcaactct tttctgtaaa attcaattgt tgtttcatga cgactttctt gtttttcaat
     2521 ttgattattt aaagtatcaa tctgattttt aaggatttct actatttgag catcgctttt
     2581 cgattcaaaa ttaatattat ccgacccttt ttttgtttgt cttcagtagt actttcaaaa
     2641 ccatatttct ttttattttc atttactctt tgaatgattt tttctatatc ggtatcttct
     2701 ttaataaacg aagtgttttc ctgttttatt gtctctatat tcaatcgctt aatattatta
     2761 aaaatcgttt gcttactaac ccctaattct tcactcaact ttttcacact tttcatatca
     2821 tcaacccttt ataaaccctt gataaaacta tattttccct tttataagtt tatttaaagt
     2881 gctataataa acttaatata acaaaaaccc caactactgg taa
//
LOCUS       DAKSQE010000184         2753 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.186, whole genome shotgun sequence.
ACCESSION   DAKSQE010000184 DAKSQE010000000
VERSION     DAKSQE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.186"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            222..1535
                     /gene="murT"
                     /locus_tag="O0V33_002611"
     CDS             222..1535
                     /gene="murT"
                     /locus_tag="O0V33_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HCX8977196.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNGDDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISRFDVAPFLHLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQAIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
     gene            1537..2268
                     /locus_tag="O0V33_002612"
     CDS             1537..2268
                     /locus_tag="O0V33_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCX8977197.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHNFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEKQAKQVLIDRANRQKKSR"
     gene            2354..2463
                     /locus_tag="O0V33_002613"
                     /pseudo
     CDS             2354..2463
                     /locus_tag="O0V33_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500597.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2492..>2753
                     /locus_tag="O0V33_002614"
     CDS             2492..>2753
                     /locus_tag="O0V33_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977198.1"
                     /translation="MKPTKVILKDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEIKFEFALFDSDMDNLKYIEYDNPVTEIYFIEPDLHFTII"
BASE COUNT         1050 a          341 c          522 g          840 t
ORIGIN      
        1 ttgtaaataa taataattat aatttaatga ctgattttag cactataatt gagaaaatgt
       61 atcatttacc gagatttaat tgagaataaa tatcattttt aattacctaa atgagaatga
      121 ttataattta gaaacactgt atcaatttgt gattgagaaa cttttttgaa gttgccttgt
      181 catgttataa tgatgcgagt ggaaatttga ggaggaaaca catgagacag tggacggcaa
      241 tccatctagc gaaattggcg cgtaaagcaa gtagagcagt aggtaaaaga ggaacagatt
      301 tacctggaca aatcgctaga aaagtggata cagatatatt aagaaaatta gcagagcaag
      361 ttgatgatat tgtatttatc agtggaacaa atggtaaaac aacgacttca aacttaattg
      421 gacatacttt aaaagcgaat aatattcaaa ttatacacaa taatgaaggt gctaatatgg
      481 ctgcaggtat aacttctgca ttcatcatgc aatcaacacc taagactaaa attgcggtaa
      541 tcgaaattga tgaaggttcg attccacgtg tgttaaaaga agttacacct tcaatgatgg
      601 tatttactaa tttctttaga gatcaaatgg atcgtttcgg tgaaattgat attatggtta
      661 ataacattgc agagacaatt agtaataaag gcatcaaatt attgctaaat ggtgatgatc
      721 catttgtgag tcgtttgaaa atcgcaagtg atacgattgt gtactatggt atgaaagcac
      781 atgctcatga atttgaacaa agtacgatga atgaaagtag atattgtcca aactgtggtc
      841 gcttattgca atatgattat attcattata atcaaattgg tcattatcac tgtcagtgtg
      901 gtttcaaacg agagcaagca aaatatgaaa tatcaagatt tgatgtggca ccgtttttac
      961 atttaaatat caatgatgaa aaatatgata tgaaaatagc aggtgacttt aacgcttata
     1021 acgcgttagc agcatataca gttttaagag agctagggtt aaatgaacaa gcaattaaaa
     1081 atggctttga aacgtataca tcagacaatg gtcgtatgca gtactttaaa aaagaacgaa
     1141 aagaagcgat gatcaattta gctaaaaacc ctgcaggaat gaatgcaagt ttatcagttg
     1201 gtgaacaatt agaaggcgaa aaagtgtatg ttatttcgct aaatgataac gctgcagatg
     1261 gtcgagatac ttcatggatt tatgatgcag atttcgaaaa attatctaag caacaaattg
     1321 aagctatcat cgtgacaggt acacgagcag aagaacttca attgcgattg aagttagcag
     1381 aggttgaagt accaattata gttgagcgtg atatttataa agcaacggca aagactatgg
     1441 attataaagg tttcacagtt gcaataccaa actatacatc attagcgcct atgcttgaac
     1501 aattaaaccg ttcgtttgaa ggaggtcaat cataatatgc atgaattgac tatttatcat
     1561 tttatgtcag ataaattgaa tttatacagt gatataggaa atattattgc tttaagacaa
     1621 cgtgcaaaaa aacgaaatat taaagttaat gtcgtagaaa tcaatgaaac agaaggtatt
     1681 acctttgatg aatgtgatat tttctttatc ggtggtggaa gtgatagaga acaagcatta
     1741 gcaacaaaag aactaagtaa aattaagaca ccacttaaag aagcgattga agatggtatg
     1801 ccgggattaa cgatttgtgg aggctatcaa tttttaggaa aaaaatatat cacgcctgat
     1861 ggtacagaat tagaagggtt aggtatttta gatttttata ctgaatcaaa gacaaaccga
     1921 ttaacaggag atattgttat cgaaagtgat acttttggaa ctattgtagg ttttgaaaat
     1981 cacggtggta gaacatatca taatttcggt acacttggtc atgttacttt tggttatggt
     2041 aataatgatg aagataaaaa agaaggcatt cattataaaa atttattagg tacttattta
     2101 cacggaccaa ttttacctaa aaattacgaa ataactgatt atctgttaga aaaagcttgt
     2161 gaacgtaagg gtattccgtt tgagcctaaa gaaatagata atgaagcgga aaagcaagcg
     2221 aaacaagtat taatagacag agcaaataga cagaagaagt ctcgttaact ctgaacatcg
     2281 catcaatggg tttaaaattg ataaacgatg aagtttagta attaatcata tatgtataaa
     2341 cacacacatt attttggatg gaaacaacca aattgatgtg tgttttttgt tctagtgaat
     2401 aattattata caatgagtat ctatcctaga attatcaata gtaatggtga ttatgcaaca
     2461 tgaaaaaatg aatgatgaaa ggaatttgac gatgaagcct actaaagtga tattaaaaga
     2521 tgcatcttat ttacatagca aaacatcgat aacatttatt ttaaaagatg tagttatcga
     2581 agaagataat aaaatttatt atttcgacac tagtgcaact ttcgaagatc aagaaattaa
     2641 atttgaattt gcactttttg atagtgacat ggacaattta aaatatattg aatacgacaa
     2701 tccagttacc gaaatatatt ttatagaacc tgatttacat tttacgatta ttg
//
LOCUS       DAKSQE010000185         2734 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.187, whole genome shotgun sequence.
ACCESSION   DAKSQE010000185 DAKSQE010000000
VERSION     DAKSQE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.187"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..573)
                     /locus_tag="O0V33_002615"
     CDS             complement(<1..573)
                     /locus_tag="O0V33_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977199.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRISYTESQIKVYRTKTQYTDLLFL
                     YLEHAFLSQDFFDIPSIHSDLDDILVNMFLY"
     gene            complement(587..1378)
                     /locus_tag="O0V33_002616"
     CDS             complement(587..1378)
                     /locus_tag="O0V33_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCX8977200.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQDDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
     gene            complement(1559..1762)
                     /locus_tag="O0V33_002617"
     CDS             complement(1559..1762)
                     /locus_tag="O0V33_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977201.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            complement(1791..2600)
                     /locus_tag="O0V33_002618"
     CDS             complement(1791..2600)
                     /locus_tag="O0V33_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCX8977202.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNCEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSSKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKIGTGGLGGEIHLMPVETEMTMPAYGAVDQV
                     EFETKDVDFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
BASE COUNT          854 a          477 c          375 g         1028 t
ORIGIN      
        1 atataagaac atattaacta aaatatcatc taaatctgaa tgtatagatg gtatatcaaa
       61 gaaatcttga cttaaaaatg catgttctaa atacaaaaat aataaatctg tatattgtgt
      121 tttagtacga tacactttaa tttgagattc cgtatatgat atccgtgtat ctaagcgaag
      181 atcaattaat ttagcagtac ttgggcgctc aactttaata gaatttaata cgcgcatatc
      241 ttctaataat ttaaaaagtt gttgataaaa tttagggtgt ttaaaagttt tatcttgtac
      301 tacttcattt acaatttgta catccatcat atgataaccg taagcagcta acataacatc
      361 tgtttttaaa ccagccattt cgatatggct tggtcgatgt gaccaaaacc aactcgtaat
      421 caaaacattt tgaacaggat tatagtaagg gaatttttgg attttaactt gtgtttgttc
      481 attttttaat aagagtcgag ctaagtcttg caacatcatt acttgcttgg catctaactg
      541 ttcgtcgtta aattttatga aacgatcact catgttttat ccctctttaa aagtttagtt
      601 ctacagcatt atatattgct tgttgttcac gttcatcttc caatttatca ataattgtac
      661 gtttaattgc acgttcaact ggcattacag tgattaaatc acacaagtct aataatgcac
      721 gtatactagc ggcttcttca gaaatttgtc cctgcttaga catagtacgt aaatcttcgt
      781 taaacttaat aatttgttcg atttgtttat catcttgtaa taaactttgc tctttaatta
      841 cattttttaa aatgtccccg tcaatatagt caacgtgaat aacaacaaag cgatttttta
      901 gtgcttcatt cattggcaat gtaccaacat aaccttcatt tatcgctgca ataacgttaa
      961 atcctggtac agctttgatt acttcaccag tgtatggatt cgtaatttga cgacgataat
     1021 ctaagacccc atttaataca ggtaatgttt caggtttagc catatttatt tcatcaatat
     1081 ataaaatatg cccctctttc atagctttaa taactggacc atctacaaag acaatttctt
     1141 gttgtccttc cgcatttgtt ttaattgttt taaagcctaa taagctttct gtatctaaat
     1201 caacagaaca gttgacttga tgcatgggtg tatcaacaac ttcacttaat gtttctgcca
     1261 acttcgtttt ccctgaacct gttggacctt taagtaaaat atttttattt aaatcgaata
     1321 atgccttcgc atcattgaaa actgttgaat ctgaattctt ataatgtttt agtgccattt
     1381 taaaacgttc tcctttaaat aaattaaaaa actaagacat ggaataagtt ctccaaatgc
     1441 cttagtttac tattctattc tgatataaaa cgcaatgctt agtatgcgaa ttgtttatat
     1501 tttaccaaat ttactaattg aaaatctcat attaggattc atttcaataa ttatgcgttt
     1561 aaacttcttt gaaatatgtt ttatagtaac cgccaactaa tcctgaatta tctattattt
     1621 gataatattc ttcagtttcg ttgataacat catattgttt gcctacagtt aacatgtcag
     1681 aaactgtaaa tttctttgca tctgtattaa tgacttctac tttcttgatt ggcgtacgtt
     1741 ctttccaagt ttcatgtaac ataaattttg tcctcactat cgtaaaaaaa ttatttttgt
     1801 aaagtgaata taaacgataa tccactgttt tcagtaattc taattgattc aatatcatct
     1861 tgctcaagtg tttgatatgg tatttccact tcatctaagc gcgatttcgc ttgattaaag
     1921 aaatctacat ctttcgtttc aaactcaact tgatcgactg cgccatatgc cggcattgtc
     1981 atttcggttt ctacaggcat taaatgtatt tcgccaccta gaccacccgt tccaattttg
     2041 aatacttgga catgatagtc agcattgtcg aagggttggt attctgcaaa tacttcaagt
     2101 ccgaatatat ttgttaaaat ttgacctgta atatctacat gattcacttt aagaatcact
     2161 ggccctaaac cttgcacttg atgtaacgga ttgaccgcac tttcgaaaga aggcatacct
     2221 aaaccaacgc cataattatt ctcgtttgaa tatattgaga aaatatgacc attgttatct
     2281 tcgagactga aatatttatt gccatttaat tctttaactg ttgtaaatgg aatatcctta
     2341 ctcgataata tttccgcata ctcctctaga cctgagtcag ttggtgtacg taatccaata
     2401 cccacaaagt gagattcttc taattgctca cttggaactt gtataaattg aagtcttgtt
     2461 cctgggctta tatctgcatc gccgaaacga attgagtttt caagaagttc ttcacaattt
     2521 aatcctaata tgtcaaccat gaaatgtttt gtctgttcta tatttgttgt acctaatgtt
     2581 atacttctaa gtccacacat tatagccacc tctatcttta tctttactat tatattataa
     2641 cttattttaa tcataaatta tagaatatcg gtcagttgat tttgattata gattaatatg
     2701 tgaaaaatgt atttctttaa aagaactatt tcat
//
LOCUS       DAKSQE010000186         2703 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.188, whole genome shotgun sequence.
ACCESSION   DAKSQE010000186 DAKSQE010000000
VERSION     DAKSQE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.188"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(278..1078)
                     /locus_tag="O0V33_002619"
     CDS             complement(278..1078)
                     /locus_tag="O0V33_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCX8977203.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKLRFEANFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYKGNELYNYKSFNRQVFQQVVDYLNMNQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGHDSIDIIMPNMTKGQALKRLLDKWGMSSSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLKK"
     gene            complement(1308..1928)
                     /gene="srtA"
                     /locus_tag="O0V33_002620"
     CDS             complement(1308..1928)
                     /gene="srtA"
                     /locus_tag="O0V33_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HCX8977204.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDNKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVEVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
     gene            2122..2613
                     /locus_tag="O0V33_002621"
     CDS             2122..2613
                     /locus_tag="O0V33_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCX8977205.1"
                     /translation="MIRCAKKEDLNAILAIYNDAIINTTAVYTYEPQTIDERVAWFET
                     KQRKHEPIFVFEENGSVLGFATFGSFRPWPAYLYTIEHSIYVDASARGKGIASQLLHH
                     LIVEAKAKGYRALVAGIDASNKASIQLHQKFAFKHAGTLTNVGFKFNQWLDLAFYELD
                     LQD"
BASE COUNT          836 a          501 c          380 g          986 t
ORIGIN      
        1 tacaaaagta gaagctaatg ccaataggta tacatttgcg tggaagaaaa gtattcaaaa
       61 tcatgaattg ttttattttg tatatctgta tcaaaatcga tttgattaaa atccgcttta
      121 ttttatcatc tattcaaatg attttagtgc gcttatttta ctatggcatt attgcctaac
      181 ctgtttgaga tatattaata tttgatgatt cgtattcaaa gttttcattt aacatgtatt
      241 tagtatcatg atagctgctt cattgataat ctacttccta ctttttcaac acttcttgtt
      301 cgataatcgt taaaacaccc tgcttatcat tacttggagc gacagcagag gcaatattga
      361 acaattcttc atcatgactg ttttccatca cataactatg cttagcgaat gctagcatat
      421 ctttatcatt attcgcatct ccgaaggcca tgagctctga agaagacatt ccccatttat
      481 ctaacaatcg ttttaatgcc tgccccttag tcatgtttgg cataataata tcaatgctat
      541 cgtgcccact tgagacaagt ttaatatcat tgctgaattg cgttgctact tcttcgtcaa
      601 cattcggatg cgtctcacga ttaatattaa atgctatttt gacataatca tcctcaggta
      661 attgttgtag actgtcaatt tcttttaact gatgataata aaatctcgta tcttctttaa
      721 atgcttcaga agtatgtttt aaaatatacg cgcttttcaa accacagatg acgagttgat
      781 caatactttg gttcatattt aagtaatcga caacctgttg aaacacctga cgattaaagc
      841 ttttataatt atataactca ttgcctttat aaataactgc accattttca gaaataaaat
      901 acatatctct atccccgaaa atagacttca acttcgcata ttgattacca ctcgcagcaa
      961 taaatgtaat atctctattt ctaagttcag taaaattcgc ttcaaatcta agtttatcgt
     1021 atgtcttttt tgaatctaaa aacgtcccgt ccatatctac cgcaatagcc ttaaccatga
     1081 ttatcctcct cattgctctt ttagtcgtct tcattatatc catctcatga caagcaatga
     1141 agtttgtcat accacaatga acctatattc acattttgaa attgctttca tatatgcaaa
     1201 acacattaca ctggatatta aattactttt tacctcacac caaacaaaaa aacagtctct
     1261 caatcaagac tgttttctac ttaatattca tccattatca caatggatta tttgacttct
     1321 gtagctacaa agattttacg tttttcccaa acgcctgtct tttcattgta atcatcacaa
     1381 gtaattaatg ttaattgttt atctttacct ttttgttcat ctagaacttc tacatctgta
     1441 ggcttaacat ctcttatact tgtcatttta tacttacgtg tttcattacc aactttaaag
     1501 taaaccatac tacctttttt ggctgcttta agatttgtaa attgatagtt cggacggtca
     1561 atgaaagtgt gtcctgcaat tgaaatattt tgatcatcta gtgattcatt ttcttctgca
     1621 aagcttacac ctctatttaa ttgttcaggt gttgctggtc ctggatatac tggttcttta
     1681 atatcagcat ctggaatttc aatatagcct gccacttttg atttatcttt cggaatctga
     1741 ggtttagctt gctgcttatt gtctttactc gcctgttctt ttacattttt atcatattgt
     1801 tcaatctttt catctttatc tttatcgtga agataattat cgatatgtgg tttagcaaac
     1861 aaatatgctg ccactaggat aagtactaca ccagcgattg tcattaatcg atttgtccat
     1921 tttttcatac gttaaggctc cttttataca tttcacaatg cctattaaca tttcatatag
     1981 tataccatta attataaaat gactcatagc aattcatttt ataatattaa atttgcacat
     2041 atattgtcac gttagatttc attacacata tttgcattca aataacgaaa cgcttttaat
     2101 aattactaag ggggaattgt tatgattaga tgcgctaaaa aagaggattt aaacgctata
     2161 ttagcgatat acaatgatgc cattatcaat actacagctg tttatactta tgaaccacaa
     2221 accatagacg aacgtgtcgc atggtttgaa acgaaacaac gtaagcatga gcctatcttt
     2281 gtatttgagg aaaatggaag tgtcttaggg tttgcgacgt tcggttcgtt tagaccttgg
     2341 ccagcatacc tatatacaat cgaacattct atttatgtcg atgcttcagc tagaggaaaa
     2401 ggtattgcta gtcaattgct acaccattta attgtggaag caaaagctaa aggttatcgt
     2461 gcactagttg caggcattga tgcttccaac aaagcgagta ttcagttgca tcaaaagttt
     2521 gcttttaagc atgccggcac actgaccaat gtaggtttta aatttaatca atggttagat
     2581 ttagcatttt acgaattaga tttacaagac tagtaatatt tgaatcacat aatataaaca
     2641 agacaaccat gttaattcac ttaacataac aagccaacat ataaaatttt aaacttctca
     2701 ggg
//
LOCUS       DAKSQE010000187         2576 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.189, whole genome shotgun sequence.
ACCESSION   DAKSQE010000187 DAKSQE010000000
VERSION     DAKSQE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.189"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            34..252
                     /locus_tag="O0V33_002622"
     CDS             34..252
                     /locus_tag="O0V33_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977206.1"
                     /translation="MENQKQGNGLKIATWVFIVLTVVTPLFGIGSIVCSINYKKYDAE
                     KGSKLLKIAIIVTIIVFVLNLLAYLGLR"
     gene            384..884
                     /locus_tag="O0V33_002623"
     CDS             384..884
                     /locus_tag="O0V33_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCX8977207.1"
                     /translation="MTFEEKLSQMYNEIANEISGMIPIEWEKVYTMAYIDDEGGEVFY
                     YYTEPGSNELYYYTSVLNKYDISESEFMDSAYELYKQFQNLRNIFKEEGYEPWTSCEF
                     DFTKEGELKVSFDYIDWINTEFDQLGRQNYYMYKKFGVIPEMEYEMEEVKEIEQYIKE
                     QDEAEQ"
     gene            895..1395
                     /locus_tag="O0V33_002624"
     CDS             895..1395
                     /locus_tag="O0V33_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCX8977208.1"
                     /translation="MTFEEKLSEMYNEIANKISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTKINSDELNYYTDIPKEYNISVQVFDDLWMDLYDLFEELRNLFKEEGHEPWTSCEF
                     DFTRDGKLNVSFDYIDWANSEFGPMGREHYYMYKKFGIWPEKEYAINWVEKIKDYVKE
                     QEEAEL"
     gene            1406..1894
                     /locus_tag="O0V33_002625"
                     /pseudo
     CDS             1406..1894
                     /locus_tag="O0V33_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
     gene            1905..2393
                     /locus_tag="O0V33_002626"
                     /pseudo
     CDS             1905..2393
                     /locus_tag="O0V33_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
BASE COUNT         1039 a          239 c          522 g          776 t
ORIGIN      
        1 tgaaagacaa aataatgagt aggagttaat gagatggaaa accaaaaaca aggcaatggc
       61 ttaaaaattg caacatgggt atttattgta ttaacagtag ttacaccgct atttggtatt
      121 ggaagtattg tttgtagtat taattacaaa aaatacgatg cagaaaaagg ttcgaagtta
      181 ttgaaaattg caattatcgt aacaataatt gtttttgttt taaatttatt agcatattta
      241 ggtttaagat aagtaaaatg aatttgaaga agcattaagc gacatttggg tgttgtttaa
      301 tgctttttat ttaacggaaa taagtgtaga gaataaatta atagcttaat caagagatat
      361 ttcgaacaca taggggcgat aacatgactt tcgaagagaa gttaagtcaa atgtacaacg
      421 agattgcgaa tgagatcagt gggatgatac cgatagagtg ggaaaaagta tatacaatgg
      481 cttatataga tgatgaaggt ggagaagtgt tctactatta cacagaacct ggaagcaatg
      541 aattatacta ctatactagt gtattaaata aatatgatat atcggaatca gaatttatgg
      601 actcagcgta tgagttgtat aaacaatttc aaaatttaag aaatatattt aaagaagaag
      661 gatatgaacc atggacatca tgcgaatttg attttacaaa agaaggtgaa ttaaaagttt
      721 catttgatta tatagattgg atcaatacag agtttgatca attgggccgt caaaattatt
      781 atatgtacaa aaaatttggg gttataccag aaatggaata tgaaatggaa gaagttaaag
      841 aaatcgagca atatattaaa gagcaagatg aagctgaaca ataggggaga taacatgact
      901 ttcgaagaaa aattaagtga aatgtacaat gaaattgcga ataaaattag tagcatgata
      961 ccagtagaat gggaaaaggt atatacaatg gcttatatag atgatggagg aggtgaagta
     1021 ttctttaatt atactaaaat aaacagcgat gaattgaatt attacaccga tatacctaag
     1081 gagtataaca tttctgtgca agtatttgat gatttatgga tggatttata tgatttgttt
     1141 gaggaattaa gaaatttatt taaagaagaa ggacatgaac catggacatc atgcgaattt
     1201 gattttacaa gagacggcaa attgaatgtt tcatttgatt atattgattg ggcgaattca
     1261 gagtttgggc caatgggaag agaacattat tatatgtata aaaaatttgg aatttggcct
     1321 gaaaaagaat atgccataaa ttgggttgaa aaaataaaag attatgttaa agagcaagaa
     1381 gaagctgaac tataggggag ataacatgac tttcgaagaa aaactaagtg aaatgtacaa
     1441 caagattgca aatgagattg ttggcatgat acctgtagaa tgggaaaagg tatatacaat
     1501 agcctatgta aatgatagag gtggagaggt cgtttttaat tatactaaac caggtagcga
     1561 tgaattgaat tattacatga atatatctag agattataat gtttcggaag aaatatttga
     1621 tgatttatgg atggaacttt atagatcatt taaaaaatta agaaatatat ttaaagaaga
     1681 aggacatgaa ccatggacat catgcgaatt tgattttaca aacgaaggta aattaaaagt
     1741 ttcatttgat tatattgatt gaaagaatac agaatttgat caattgagtc ttgaaaatta
     1801 ttatatgtac aaaaaatttg gggttatacc agaaatggaa gaaattaaag aaatcgagca
     1861 atatattaaa gagcaagaag aagctgaact ataggggaga taacatgact ttcgaagaaa
     1921 aacaaagtga aatgtacaat aaaattgcaa atgagattag tgggatgata ccagtagagt
     1981 gggaaaaggt atatacaatt gcctacctag atgatgaagg tggagaagtc gtttttaatt
     2041 atactaaacc aggtagcgat gaattgaatt attacacgga tatatctaga gattataata
     2101 tttcagaaaa aatatttgat gatttatgga tgaatcttta ttacttgttt atgaatttaa
     2161 gggatttatt taaataagaa gatttagaac catggacatc atgtgaattt gactttacaa
     2221 gcgaaggtga tttaaacgta tcttttgatt atatagattg gattaaatta ggttttggcc
     2281 catcaggaaa ggaaaactac tatatgtata aaaagtttgg tgttttacca gaaatggaat
     2341 atgaaatgga agaaattcga gcagtagaga agtatgttaa agagcaagag tagcagacat
     2401 gttataaaag gctgtgcaaa accaacctcg ttttacattt gattcaaaga agaaggtaaa
     2461 aaataagatt atttgcaact taaaaagtca attagcttat cggtatccat acatcatgga
     2521 taaataagtt caactaatta acaaatcacg atataaattt aaaattgtca ttaatt
//
LOCUS       DAKSQE010000188         2549 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.190, whole genome shotgun sequence.
ACCESSION   DAKSQE010000188 DAKSQE010000000
VERSION     DAKSQE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.190"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            138..>2549
                     /locus_tag="O0V33_002627"
     CDS             138..>2549
                     /locus_tag="O0V33_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 8 family protein"
                     /protein_id="HCX8977209.1"
                     /translation="MTNKMKKWQKLSTITLLMTGVIALNNGEFRNVDKHQIAVADTNV
                     QTPDYEKLKKTWLDVNYGYDQYDENNQDMKKKFDAKEKEAKKLLDDMKTDTNRTYLWS
                     GAENLETNSSHMTKTYRNIEKIAESMQHKNTVLKTVENKLKIKEALDWMHKNVYGKNP
                     SQKVEDLTKNRKGQTTPKNNSLNWWDYEIGTPRALTNTLLLMDDMLTKDEMKNYSKPI
                     STYAPSSDKILSSVGESEDAKGGNLVDISKVKLLESVIEEDVDMLKKSIDSFNKVFTY
                     VQDSATGKGRNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKESPFKTTQDNA
                     TLSNWIDEGFMPLIYKGEMMDLSRGRAISRENETSHTASATVMKSLLRLNDTMDDSTK
                     TRYKQIVKTSVNSDSSYNQNNYLNSYSDIAKMKKLMNDSTISKNDLTQQLKIYNDMDR
                     VTYHNKDLDFAFGLSMTSKNIARYENINGENLKGWHTGAGMSYLYNSDVKHYRDNFWA
                     TADMTCLPGTTTLNDMPSTNTKNDKSFVGGTKLNNKYASIGMDFENQDKTLTAKKSYF
                     ILNDKIVFLGTGIKSTDSSKNPVTSVENRKANGYKLFKDDIEITTSDVNAQETHSVFL
                     ESNDTKKNIGYHFLDKPKITVKKESHTGKWSEINKSQKKDDKKDEYYEVTQTHNTSDS
                     KYAYVLYPGLSKSDFKSKNNNVSIVKQDEDFHVIKDNDGVFAGVNYSDNTKSFDINGI
                     TVELKEKGMFVIKKKDDKAYKCSFYNPETTNTASNIESKIFIKGYTITNKSVINSNDA
                     GVNFEL"
BASE COUNT         1010 a          363 c          441 g          735 t
ORIGIN      
        1 tacattttta gatattaaat aaatctcatg gtgactgatg gtttccatgc atcaacgaat
       61 ttcccaatcc tttggaaagt tgaatcatta attaatccta atatcaattg cacacacgca
      121 aaaaaggaga catgtcaatg acaaataaaa tgaagaaatg gcaaaaatta tccaccatta
      181 cgttattaat gaccggagtg attgctttaa ataatggtga atttagaaat gttgataaac
      241 atcaaatcgc tgtggctgat acgaatgttc aaacgccaga ttatgaaaaa ttgaagaaga
      301 cgtggctcga cgttaactac ggttatgatc agtatgatga gaataatcaa gatatgaaga
      361 agaagtttga tgctaaagaa aaagaagcca agaagttact tgatgacatg aaaactgata
      421 cgaatagaac atatttgtgg tcaggagctg aaaaccttga aactaattct tctcacatga
      481 caaaaaccta tcgtaatatc gagaaaatcg cagaatcaat gcaacataag aatacggtat
      541 taaaaacagt tgaaaacaag ttgaaaataa aagaagccct agattggatg cacaaaaatg
      601 tttatggcaa gaatccttct caaaaagtcg aggatttaac taaaaatcgt aaggggcaaa
      661 ctacacccaa gaataactca ttgaattggt gggattatga aattggtacg ccaagagcat
      721 taacaaatac actacttcta atggatgata tgctcactaa agatgaaatg aaaaattatt
      781 caaaacctat tagtacatat gcaccatcca gtgacaaaat tttatcttct gttggtgaat
      841 cagaagatgc taaaggtgga aatttagtgg acatttctaa agtaaaactt ttagaaagtg
      901 ttattgaaga agatgtagat atgttgaaaa agtctataga ttcttttaat aaagtgttca
      961 cttatgttca agattctgcc actggtaaag gtcgcaatgg attctataaa gatggctctt
     1021 acattgatca tcaagatgtc ccttacactg gtgcttatgg tgttgtacta ttagagggta
     1081 tttctcaaat gatgccgatg ataaaagaat ctccttttaa aactacacaa gataatgcta
     1141 cattaagcaa ttggattgac gaagggttta tgccattaat ctataaaggt gaaatgatgg
     1201 atttatcacg aggtagagct atcagtcgtg aaaatgaaac gagtcataca gcgtcagcga
     1261 ctgtaatgaa atcattgttg agattgaatg ataccatgga tgattcaaca aaaactagat
     1321 ataagcaaat cgttaaaact tctgttaatt ctgattcaag ttacaaccaa aataattatt
     1381 taaattcata ttcagacata gctaaaatga aaaagttaat gaatgatagt actatttcta
     1441 aaaacgattt aacacagcaa cttaaaatat ataatgacat ggatcgtgtc acctatcaca
     1501 ataaagacct ggactttgca tttggtttaa gtatgacatc gaaaaacatc gcacgatacg
     1561 aaaatatcaa cggagagaac ttaaaaggtt ggcacaccgg tgcaggcatg tcttatttat
     1621 ataacagcga tgtcaaacac tatcgcgata acttctgggc aacagccgat atgacttgtc
     1681 ttccaggcac tactacttta aatgatatgc catctactaa tactaagaat gataaatctt
     1741 ttgttggcgg gacaaaatta aataataaat acgcaagcat cggtatggat tttgaaaatc
     1801 aggacaaaac tttaactgcc aaaaaatcat atttcatatt aaacgataaa attgtcttct
     1861 taggaactgg cattaaaagt actgattcat caaagaatcc agttacaagt gttgaaaatc
     1921 gcaaagcaaa tgggtataaa ttatttaaag atgatattga aattaccact tcagatgtta
     1981 atgctcagga aacccattca gtctttttag agtccaacga tactaaaaag aacattggtt
     2041 atcatttctt agacaagcca aaaataactg taaaaaaaga aagtcatact ggtaagtgga
     2101 gtgaaattaa taaaagtcaa aaaaaagatg acaaaaaaga tgagtattat gaagtaactc
     2161 aaacacataa tacatctgac agtaaatatg catatgtttt gtatcctggt ttatcaaaaa
     2221 gtgattttaa atcgaagaat aataatgtaa gtattgttaa acaagatgaa gattttcatg
     2281 tgataaaaga taatgatggc gtatttgctg gggttaatta tagtgataat actaaatctt
     2341 ttgatataaa cggaattact gttgaattaa aagaaaaagg catgtttgta attaaaaaga
     2401 aagatgataa agcatataaa tgtagcttct ataatcctga aactacaaat accgcttcaa
     2461 atatagaatc aaaaattttt attaaaggtt acaccataac taataaaagt gtcataaact
     2521 ctaatgatgc tggtgtaaac tttgaatta
//
LOCUS       DAKSQE010000189         2542 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.191, whole genome shotgun sequence.
ACCESSION   DAKSQE010000189 DAKSQE010000000
VERSION     DAKSQE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.191"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            54..2206
                     /locus_tag="O0V33_002628"
                     /pseudo
     CDS             54..2206
                     /locus_tag="O0V33_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            2352..>2542
                     /gene="sarX"
                     /locus_tag="O0V33_002629"
     CDS             2352..>2542
                     /gene="sarX"
                     /locus_tag="O0V33_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HCX8977210.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELD"
BASE COUNT         1003 a          297 c          388 g          854 t
ORIGIN      
        1 aatattatag ggaatgataa aagtggtact taaatataga aagaggtaaa gttatggcaa
       61 aatcatgctt gcatatactt actaataatg aatatgcgac aacgcgttgc caagatggca
      121 tagtcttatt ttggccaatt gacggggaaa tcgaactaca aaaatttcgt aaaagtaaaa
      181 taattgaaga tgatatatat atattattaa tcatctggat gtatttagta ttaagaataa
      241 taaaaaaacg atcatgttgt atttgagtag cgattggttt gcggaattag gctttacttt
      301 ctttaattac cactatacag caaagttaat taaatcatct tataatttga aatgtttact
      361 attaaaattg acttatcgat accttgataa tcaacctctc aatgacgctg atgttagaaa
      421 attacaggat attattaaaa ttattgcaaa agaagcaagt atggataaaa agattgcgca
      481 aaatcaatat cgatatgcct attatggtga tttgcgtgac gagctcgaat atatttatca
      541 aaatgtaaat caacgattga cattaaaaag tgtcgctgat aaattatttg tctcaaagtc
      601 aaatttgtca tcacaattcc acttacttat gggcatgggt tttaaaaaat atattgatac
      661 tttgaaaatt ggtaaatcga ttgaaattct acttactact gatagtacta taagcaacat
      721 aagtgaacat ttaggtttta gtagtagctc aacttactct aaaatgttta aaagttatat
      781 ggatataaca ccgaatgaat atcgtaattt atcaaaattt aataaatcta taatgcttaa
      841 tccagagcca cttgtaggcg gtatggcgca agaagtaaaa gaagtcatat tgaattatat
      901 tgatcattat aaaaatcacc taactgatgt tatacatatt gacgaagaca aatttgaaat
      961 acctaagtta tttcaaacgg ttattcaaat taatacttat acagaaatga aactagtttt
     1021 cttagaagga ctttttaaaa ccttattgaa taagaatagc caagttgtct ttttcatcat
     1081 gccatcgatt ttaaaaagta aaaataccat gtccgaagaa gaaaagttta caatcattaa
     1141 aaccataatt gaaagtaatt taaagatagc atttaatata aatgatattg aaacgactta
     1201 ttatgttgaa gaagctttta tgaatgttat tagacaatta tctccaaacg aaataaatca
     1261 tcataataat tacgaagtgc attttgtttt tgatttatca ttgatggaaa ttaggacaat
     1321 ttaccgtatg attttaaaac tacataacat catgttaaat gtgaaattag gattgaatat
     1381 tacctgttta tttgataaac cttcagtttt taaatcacta gtatcacaaa taaagcgact
     1441 taaattcgat tcgttaataa tagataatgc aaatttaagt agcccttatt tgatggggga
     1501 aagtgatgag ttactattga aaaatatttt gcattttaaa aatttaaaac aagttattaa
     1561 cgaattggat atagaacaag aaaagctgat ttttctaaat gttgaaaatc ataaactgct
     1621 taataataaa gaacgagatt taagtaatag tgctccatta atttataaga cattaagtgc
     1681 gctgtatcat aactttcatg gttttggatt aaacattttt gataatcatc aaacatttaa
     1741 tgcgatgcat ctatatgata aaaatggatt taaaacaaca ctaggtctta tattagaaaa
     1801 gtttatcgaa tatgtctcga aaccaaaata cgaaaacagt tattattcta tttttgatat
     1861 agagaattat tattgtctgg ttatttatga ttggcgagtg atagagagcg agacaattat
     1921 gagcaatttt gaagatagtc aagtttatat taatttcaaa aataatattt taaacgataa
     1981 atatctaatt gtaatagaaa cgttggatga aaatagtggg aacattaatc atttgatctc
     2041 taaagaatta agagataaat atgaatggaa ccccagttta ctctctaaaa ttgacaacta
     2101 ccttaaaccg gcaatcgaga ttaaagagca taactttagt aataattcct taaatattaa
     2161 cgttactttt aatgcgttat acataattaa aataggaaaa aaataacacc ttgatatgta
     2221 ttgcaaaatt taatttgcat ttgttggtaa ttttgacaac gtattaaaaa caaatgagaa
     2281 agacaatggc agaaaaattt aagataataa tgacagaagc attgtcttta tatatttggg
     2341 ggtgcaacat tttgaatact gagaaattag aaacattgct tggcttctat aaacaatata
     2401 aagcattatc tgaatatatt gataaaaaat ataagttgtc gctaaatgat ttagcagtct
     2461 tagatttaac gatgaagcat tgcaaagatg agaaagtact tatgcaatca tttttaaaaa
     2521 ctgcaatgga tgagctagat tt
//
LOCUS       DAKSQE010000190         2457 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.192, whole genome shotgun sequence.
ACCESSION   DAKSQE010000190 DAKSQE010000000
VERSION     DAKSQE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.192"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..1435
                     /locus_tag="O0V33_002630"
     CDS             <1..1435
                     /locus_tag="O0V33_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCX8977211.1"
                     /translation="LYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLV
                     LTTIFFIANILIIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPL
                     TLKGKKGYKFSIFMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYT
                     LEISPNIRNSEEKQILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFI
                     NNNFLNFYKNINHDFKMIVNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKV
                     DVQVLSKNVSVFSFDRTTNLKFGYLDSPIVMLLTPDDLTGDFYLAAVSQGGYLFKDLD
                     TLKQLLKDNHLEEEISGITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFET
                     IQYFSWNKKQLLLRKIHGYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMS
                     IAIIQHLLQIAYIKYLEKYFKDLMREI"
     gene            1439..1750
                     /locus_tag="O0V33_002631"
     CDS             1439..1750
                     /locus_tag="O0V33_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCX8977212.1"
                     /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVP
                     KDTQDYRNITVYSKNGEKKSYKLDFLGYDPTKEYVKVNHKGKYVRSIEYITKDIPSFL
                     K"
     gene            1762..2388
                     /locus_tag="O0V33_002632"
     CDS             1762..2388
                     /locus_tag="O0V33_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8977213.1"
                     /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLL
                     NIIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLGLALKFK
                     KNKKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTG
                     SLDTKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS"
BASE COUNT          946 a          299 c          362 g          850 t
ORIGIN      
        1 attatatctt ttgtacttct tatatgacag aggatataat ttgaaatttt atgctattca
       61 aaagctacat ggcttttcaa ctacacgcat tatttttcat aatattcata taaaaatcat
      121 ttattggcta gtacttacca ctattttctt tattgctaat atattaatca ttcatatagc
      181 caatttagaa ttggatttct taatgtttat tcgtagattt atcgtgttgc tttttgtttt
      241 tgcttgtatt actacattgt tgtggctgat ttcgtatagt cttcttttga agttaagtat
      301 tccattaacc ttaaaaggga aaaaaggata taagttttca atatttatgg gtaccttcac
      361 caaatgtatc atggtgctaa tactatcttt tttactggca caaaatttaa atgcttatac
      421 gaagttaaag aatatatatg actctgaaaa aatctggcaa aagttagata attactatac
      481 attagagatt tctcctaata ttagaaattc agaagaaaaa caaattttag aaactaatat
      541 atacaattta attaagaaaa gcgaacaact tggtggattg cttttaaaga ataataatat
      601 agccaatccc gataaaaaca attacattcc tgaaaatggc aatgtctttt ttatcaataa
      661 caattttctg aatttctata aaaatataaa tcatgatttt aaaatgattg tcaatcattc
      721 ggataaaata aacgtgttta ttcctccaag gttacaatat caaaaatctg aaattcaaaa
      781 aaatcaccaa ggatggattg actttcaaaa gcaacaaaac aaaaaagtag atgtgcaagt
      841 actgtcaaaa aacgtatctg ttttttcttt tgatcgcaca actaatttga aatttggata
      901 tctagattct ccaatagtaa tgttgttgac acctgacgat ttaacaggag atttttattt
      961 agctgctgtt tctcaaggtg gatatctatt taaagattta gatacactta aacagttgct
     1021 taaagataat cacctagaag aagaaattag cggtataact aattacaagg attctgtgtt
     1081 aaatgaatta aatgaaacga aaacaaaaat gataataaca atagttacta ttattattaa
     1141 cgttttcatt cttttgatag ctactgtatt tgaaacaatt caatatttta gctggaataa
     1201 aaagcaacta ttattaagaa aaattcatgg ttattcatta ttcagcagta atgtacgtta
     1261 tttaacaatt tctattttac tttctattat gttagcttac actacgcata ttttatttgg
     1321 ttcaaagata ttactattca taataatgtc tattgctatt attcaacatt tacttcaaat
     1381 tgcttatatt aaatatcttg aaaaatactt taaagattta atgagggaga tttaaacaat
     1441 gaaaagaact ttaattattg taacaattgt actattaatc atcatactta gtctgttatt
     1501 ggttagaaat gagcatttag atcgttttaa cccactgtta aaagaaaaag tatcttatgc
     1561 caaagttcca aaagataccc aggactatag aaatataact gtatactcta aaaatggaga
     1621 aaaaaagagt tataaattgg attttttagg ttatgatcct acaaaagaat atgtgaaagt
     1681 aaatcataaa gggaaatatg tgcgttcgat agaatacatt acaaaagata ttcctagctt
     1741 tttaaaatga ggtgaccgat agtgataaaa cttgaaaatg tttttgtaaa aaaaggtaat
     1801 aaaaacattt tagacggatg taactttaat tttgaaaaag gaaaatcata tgctttagtt
     1861 ggagaaagtg gcgctggaaa atctacttta cttaacataa tcgccgggtt cgaagatgta
     1921 tcacaaggaa gcatatatat tgaagataag ttactaaaaa agaaagtcga tttttataga
     1981 tatacgttag ggtatttgtt tcaaaatttt gctttacttg aaaatcaaac aatcagtcaa
     2041 aatttaggtt tagccctaaa gttcaaaaag aataaaaaag ataatatgaa cttaaagaaa
     2101 gaagtattaa aaaaggtcgg cctagattta gatatcaaga gaattgttag ttctctaagc
     2161 ggtggtgaac aacaaagagt agcattggca agattaatat taaaagatcc gaaaatcatt
     2221 ttagctgatg aacctactgg atcactagat acgaaaaatg gaaaaatagt tattgattta
     2281 ttattgaagc tacttgatga aaataaaact atgattgtcg taacgcatga tttagagctt
     2341 gcgaaacgat ttgatgtcat agtcaatatt tctgagctta gaagttaaga aatattgata
     2401 ggtatagcca tacatagaga aaaaacaata tgaaatatga ttagtcatta cttttga
//
LOCUS       DAKSQE010000191         2448 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.193, whole genome shotgun sequence.
ACCESSION   DAKSQE010000191 DAKSQE010000000
VERSION     DAKSQE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.193"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            73..1388
                     /locus_tag="O0V33_002633"
                     /pseudo
     CDS             73..1388
                     /locus_tag="O0V33_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1701..>2448)
                     /locus_tag="O0V33_002634"
     CDS             complement(1701..>2448)
                     /locus_tag="O0V33_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCX8977214.1"
                     /translation="NKKIELCTFPEFQRLTVKKLEFKYSYFDEPVLRDINLDIKKGDK
                     VAIVGKSGAGKSTLLKILSGVLEKNSGEILLNNRNINSTELNKIAAYINQSPYIFKDN
                     LYNNIFLNGGLKPEEIIDVLHQSALYNMISKYPLGLQTIVSENGSNFSGGQKQQISFA
                     RALSTRKKVFILDEPTSSLDNTTENIIMKTLKNADMTCIISAHRLSTIKQFDAILVLD
                     GGYIVEQGTHNSLMKLRGYYYKLYTSREMG"
BASE COUNT          859 a          360 c          374 g          855 t
ORIGIN      
        1 aatataaaaa aagcgcaatt atctctataa ttaaaagtga ctaaacaaat aactaaagga
       61 gataatgcgc ctatgaataa tgttatacta aatacctttg attttaaaga taaaaatatt
      121 atttttgacg gtaaacttga gaaaattgag tataaaggga gaaagtgttt cttttattac
      181 gcaaaactaa tctatacacc tgaggtgtgc ccaaactgca attgtaaaaa tattaatggt
      241 aatttaatca aaaatgggtc taagacttct agaatcacta tgcctaaaat ctccgaatat
      301 ccaacttata tcatgttgcg taagcaacgt tttaaatgta aaacgtgcga acgatacttt
      361 acagctgaaa cacctgaagt tgataaatac tgttttatat caaaaaaaca cgatcagctg
      421 tacttaataa agcagcagaa attcgttctg aaaaatctat agcgaaatcc tgctctgtgt
      481 cagctactac agtttctcgt atcattgatg aggccgctca atcgcttcat caatcgccgt
      541 attctgccct tcctgaacat atcatgatgg atgaatttaa aagtgttaaa aatgtttctg
      601 ggaaaatgag ttttatatac gctgatgcac aaacgcatca tattattgat attgttgaag
      661 atcgtagatt aagtgagttg aaaaagtatt tttatcgttt ttcacttaaa gctagaaaac
      721 gtgtgaaaac agtttcgatt gatatgcatg aaggttatat gacattgatt aaagagatgt
      781 ttccgaacgc taaaatagtc atcgatcgat tccatattgt tcaattatta aatcgtgctt
      841 taaatggtat tcgagtcact gttatgaatg aattaagaac gacgaatcaa cctttatata
      901 ataagtttaa aagatatgcg aaattactat taaaacctga agaagattta gaggcatttg
      961 aatatcgtaa agtagcttta tttaaagaat ggaagacaca aaagggtatc gcaaagtatt
     1021 tattaaatca agatgactcg ttaaatgacg cctaccaata tatcaatcag ctacgattca
     1081 aacttaaaca taatgattat gaaggcttta tttacgaatt aaaacatatg cgattatccc
     1141 aaacgcattc agttgtccaa cgagcaattg agacacttaa caaacatgta tactttataa
     1201 aaaatacatt tgattattac aatttgtcaa atggtccttt agaaggtata aataacaaaa
     1261 taaaattaat taaacgtaca tcttttggtt atggaaatta caatcatttg cgcaatcgaa
     1321 tattattatg ttcaaaactt tacgctccaa aaagtaaaaa ggaagttaag caatgtttag
     1381 ttgcctaact tccgatattg aacgcatcag tccaatttaa catagagcca aaatagagaa
     1441 ctaaagtaca acttttttgg ttcgttacag tatttttgct gaaaattgtc aatgtcaaat
     1501 tatccaagaa ttcaatgtga ttcattaaag cttaatatgt atttgtggta tgaacagatt
     1561 ttcaaatcta aaacgctaca ttgacttttt ggtatctttt taattactaa ttactgtact
     1621 ttgtcaccac attgatttgg tatgatttct ataatattca ctgcagattg atagtataat
     1681 gggagtgagt tttgtataaa ttaccccatt tctcttgatg tgtataattt atagtagtat
     1741 cctcgtagtt tcatcaaaga gttatgagtt ccttgttcta caatatatcc accgtctaaa
     1801 actaatatgg catcaaattg ttttatagta cttaatctat gggcagatat tatgcaagtc
     1861 atatccgcat tttttaaagt tttcattata atattttcgg tggtattatc gagggaactc
     1921 gttggttcat ctagtataaa aacttttttt ctagtggaga gtgctcttgc aaaagatatt
     1981 tgttgttttt gaccgccaga aaaattagat ccattctctg aaactattgt ttgtaaacct
     2041 aaaggatatt tagatatcat attgtataaa gcagattgat gaagtacatc gattatttct
     2101 tctggcttta aacctccatt gagaaagata ttattgtata aattatcttt aaaaatatat
     2161 ggagattgat ttatataagc tgcaattttg tttaattcag tagaattaat gttacgattg
     2221 ttgagtaata tttctcctga attcttttcc aaaactcctg aaagtatctt tagtaaagtg
     2281 ctttttcctg caccgctttt accaactatt gcaactttat cccctttttt tatgtccaag
     2341 ttaatatctc ttaacactgg ttcatcaaaa taactatatt taaactcaag tttttttaca
     2401 gtgagacgtt gaaattccgg aaaagtgcat aattcaattt ttttattg
//
LOCUS       DAKSQE010000192         2423 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.194, whole genome shotgun sequence.
ACCESSION   DAKSQE010000192 DAKSQE010000000
VERSION     DAKSQE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.194"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            63..383
                     /locus_tag="O0V33_002635"
     CDS             63..383
                     /locus_tag="O0V33_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCX8977215.1"
                     /translation="MQAIKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYNDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
     gene            complement(533..1072)
                     /locus_tag="O0V33_002636"
     CDS             complement(533..1072)
                     /locus_tag="O0V33_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HCX8977216.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTD
                     LEEDMPKAPRKNRNLITLY"
     gene            complement(1155..1871)
                     /locus_tag="O0V33_002637"
     CDS             complement(1155..1871)
                     /locus_tag="O0V33_002637"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCX8977217.1"
                     /translation="MTHVEVVATITPQLYIEETLIQKINHRIDAIDVLELRIDQFENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFTNDSYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIITHLQQYNKEVIISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     EPQAPGQIDVTDLKAQVTLY"
     gene            2019..>2423
                     /locus_tag="O0V33_002638"
     CDS             2019..>2423
                     /locus_tag="O0V33_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCX8977218.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVPPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDPDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLN"
BASE COUNT          801 a          421 c          366 g          835 t
ORIGIN      
        1 tttattatga tataaattta gctataataa aaattattaa actatctaga ggagttgtta
       61 caatgcaagc aatcaaaagt aatgaatcat ttaaatctgt aattaatagc gatacacctg
      121 taatcgttaa atttgaggca ggatggtgcc cagactgtcg tgctatggat ttatggattg
      181 acccaatcgt agaacaatat aatgattacc aatggtatac tgttaatcgt gatgaattag
      241 aagatgtagt tgttgaaaat gaagttatgg gtatccctag cttgctagta tttaaaaacg
      301 gcgacaaaat tgcacacctt cattcagcaa atgctaagtc acctgaacaa gttgaatcat
      361 ttttagcaga aacttttaaa taaatactaa aatacaaatg tgtatatact gcacaataaa
      421 aagctgagac tgtgattgta ccgactctag taagttagat taaatctcta actcgataga
      481 tgtccatcaa ttcatgactc agcttttatt aatgtttcta taattataca tattaatata
      541 atgtaattaa gtttctattt ttacgtggtg ctttaggcat atcttcttct aaatccgtta
      601 aatataagaa tccagcaaga acttcatctt tctttatacc aagtgttttt cttactttag
      661 gatcatagat atataatggc gatttccaac atgtacctat tcccgcttca taaagtaaca
      721 acataagatt ttgtgcatat gcaccaaatg caaagtagtt ttcatcattt tgacgttgtc
      781 ttggatctgt ttttaaaata agcaataaca tgccacctag tttcgttact gcatcataat
      841 gacattgttg cttatctaat tcattaggaa atgcaaattt agaaatatcc ttactcatat
      901 ctcctaatct gtctttcgga acatgcacaa ctctccatgg ttccctcatt ccgtgatttg
      961 gagcatcagc agctttctca attgcttgat atagcaatgc gtcatctatg tgcatatctc
     1021 ttttaaattt tttcacactt cttctattag ctattgcttg ttgtaattcc atctaaaatc
     1081 gctcctccac gaacaatgat aatacttctc aattataaga cagcacttac acaaaagcaa
     1141 cctaattcat tttattagta taaagtcact tgtgctttta aatcagtaac atcaatctgt
     1201 cctggagctt gtggttctcc gatacaacca taagtcaacg caccaccaaa aacgccttga
     1261 gccgttctac ttattagtcc aagtttagac attgaaatac caacaacttt gcagtccata
     1321 gtatctgaaa atgtagacat tgcctgcaat aaatttagca catcattttt attatgtggc
     1381 attactgcta atttaacgta ttctgggttg aatttttgca ttttaaaaaa tataaattgc
     1441 aattcatcta atggaggcgt actttcgaaa ttatgatgtg atataatcac ctctttatta
     1501 tactgttgca aatgtgtaat gattcgttga tgtttttcaa tgtcaatatc tgcttgccat
     1561 tctatatcaa tcatatctat gccattgata tttgctaagt ctgatattaa attaagatac
     1621 gagtcatttg taaattgccc atagccacct tgtaactttg tacgatacgt aaccaataat
     1681 ttgaatgaat cttgcataac cttcagcttt gtaatcattt ctgccacttg atcaactgtg
     1741 acattttcaa attgatcaat tcgtaattct aatacgtcta ttgcatcaat acgatgatta
     1801 attttttgaa ttaaagtttc ttcgatatat aattgtggcg tgatagtcgc tactacttcc
     1861 acatgtgtca tagtaaatcc ctcatttcag atactcatta tttttattgt aaacaacaac
     1921 tttttatcct tcaatcaaac aatgctataa atgaatatga ttaaggtatt ttcgaatggg
     1981 taaagcataa atgtatttta aattaggagg ttataagaat ggcaatacat tatgaaacta
     2041 aagcgacgaa tgttggcgga cgtaaaggac atgtttatac tgatgatcga gcattagata
     2101 ttgatatcgt tccgcctgct caagcagatg gtaaagctac taaccccgaa caattatttg
     2161 cagcaggtta tgcatcttgc ttcaacggtg ccttcgacct aattttaaag caaaacaaag
     2221 tgcgtgatgc tcatccagaa gtaacactaa cagtgagact agaagatgat ccagactcag
     2281 aaagtcctaa attaagtgtt tcaattgatg cgacaattaa aaatgttata tctcaagaag
     2341 aagctgaaaa atatttacaa atggctcatg aattttgtcc atattcaaaa gcgactcaag
     2401 gaaatattaa tgtcgattta aat
//
LOCUS       DAKSQE010000193         2360 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.195, whole genome shotgun sequence.
ACCESSION   DAKSQE010000193 DAKSQE010000000
VERSION     DAKSQE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.195"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            274..2343
                     /gene="eap"
                     /locus_tag="O0V33_002639"
                     /gene_synonym="map"
     CDS             274..2343
                     /gene="eap"
                     /locus_tag="O0V33_002639"
                     /gene_synonym="map"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular adherence protein Eap/Map"
                     /protein_id="HCX8977219.1"
                     /translation="MKFKSLITTTLALGVLASTGANFNTNEASAAAKQIDKSSSSLHH
                     GYSKIQIPYTITVNGTSQNILSSLTFNKNQQISYKDIENKVKSVLYFNRGISDIDLRL
                     SKQAKYTVHFKNGTKRVVDLKAGIHTADLINTSDIKAISVNVDTKKQVKDKEAKANVQ
                     VPYTITVNGTSQNILSNLTFKKNQQISYKDLENNVKSVLKSNRGITDVDLRLSKQAKF
                     TVNFKNGTKKVIDLKAGIYTANLINTGDIKNININVETKKQAKDKEAKANNQVPYSIN
                     LNGTTTNIQSNLAFSNKPWTNYKNLTAKVKSVLKSDRGVSERDLKHAKKAYYTVYFKN
                     GGKRVIHLNSNIYTANLVHAKDIKKIEVTVKTGSKANAERYVPYTIAVNGTSTPNLSD
                     LKFKGDSRVSYSDITKKVKSVLKYDRGIGERELKYAKKATYTVHFKNGTKKVINLNSK
                     ISQLNLLFVKDIKKIDVDVKTGSKAKADSYVPYTIAVNGTSTPIASKLKLSNKQLIGY
                     QDLNKKVKSVLKHDRGINDIELKFAKQAKYTVHFKNGKTQVVDLKSDIFTRNLFSVKD
                     IKKIDIDVKQHTKSNKALNKVANIATKVKFPVTINGFSNVVSNEFAFLHPHKITTNDL
                     NAKLRLALASDQGITKHDIGLSERTVYKVYFKDGSSKFVDLKAAKQDSKVFKATDIKK
                     VDIEIKF"
BASE COUNT         1018 a          299 c          365 g          678 t
ORIGIN      
        1 agatgctgta aatataattg acaaatgtaa tcggatttaa gaaatgaagg ttgtaatgac
       61 atttaagttt gaagcttggt ataaacaaaa tcaaacaaca ttttaggtat tgtaaattaa
      121 ttagttaatc aaaattaaaa gaacttttaa cagtgttaaa aaattattgt taaagataat
      181 ttattattaa tattcagtta attcaaaaaa tagagaaagt ctggctataa ttaagttgca
      241 atcacgaatt acataaaaaa ggagagataa tttatgaaat ttaagtcatt gattacaaca
      301 acattagcat taggtgtttt agcatcaaca ggagcaaact ttaatactaa cgaagcatct
      361 gccgcagcta agcaaataga taaatcatca agttcattac atcatggata ttctaaaata
      421 cagattccat atacaatcac tgtgaatggt acaagccaaa acattttatc aagcttaaca
      481 tttaataaga atcaacaaat tagttataaa gatatagaga ataaagttaa atcagttttg
      541 tactttaata gaggtattag tgatatcgat ttaagacttt ctaagcaagc aaaatacacg
      601 gttcatttta agaatggcac aaaaagagtt gtcgatttga aagcaggcat tcacacagct
      661 gacttaatta atacaagtga cattaaagca attagtgtta acgtagatac taaaaagcaa
      721 gtgaaagata aagaggcaaa agcaaatgtt caagtgccgt atacaatcac tgtgaatggt
      781 acaagccaaa acattttatc aaacttaaca tttaaaaaga atcaacaaat tagttataaa
      841 gatttagaga ataatgtaaa atcagtttta aaatcaaaca gaggtataac tgatgtagat
      901 ttaagacttt caaaacaagc gaaatttaca gttaatttta aaaatggcac gaaaaaagtt
      961 atcgatttga aagcaggcat ttatacagcg aacttaatca atacaggcga tattaaaaat
     1021 atcaatataa atgtagaaac taaaaagcaa gcgaaagata aagaagcaaa agcaaataat
     1081 caagtgccat attcaattaa tttaaatggt acaacaacta atattcaatc taatttagca
     1141 ttttcaaata aaccttggac aaattacaaa aatttaacag caaaggttaa atcagtattg
     1201 aaatctgaca gaggcgttag tgaacgtgat ttgaaacacg caaagaaagc gtattacact
     1261 gtttacttta aaaatggtgg taaaagagtg atacatttaa actcgaatat ttatacagct
     1321 aatttagttc atgcgaaaga tattaagaaa attgaagtta cagttaaaac tggatcaaaa
     1381 gcgaatgcag agcgttatgt accatacaca attgcagtaa atgggacatc aacaccaaat
     1441 ttatctgatt taaaatttaa aggtgactca cgtgtaagtt acagtgatat cactaaaaaa
     1501 gttaaatcag tattgaagta tgatagaggt atcggtgaac gtgaattaaa atatgctaaa
     1561 aaagcaactt atacagtaca ttttaaaaat ggaacaaaaa aagtgattaa tttaaactca
     1621 aaaattagcc aactgaattt gctttttgtc aaagatatta aaaagataga tgtcgatgtt
     1681 aaaacagggt caaaagcgaa agcggatagc tatgtaccat acacaatcgc agtaaatggg
     1741 acatcaacac ctattgcatc aaaactaaaa ctttcgaata aacaattaat tggttatcaa
     1801 gatttaaata aaaaagttaa atcagtttta aaacatgata gaggtatcaa tgatattgaa
     1861 ttgaaatttg cgaaacaagc aaagtatact gtacacttta aaaatggaaa gacacaagta
     1921 gttgacctta aatcagatat ctttacaaga aacttattca gtgtcaaaga tattaaaaag
     1981 atagatattg atgtaaaaca acacactaaa tctaataaag cgcttaataa agtggctaac
     2041 atagcgacta aagtgaagtt tccagtaacg ataaatggat tttcaaatgt agtttcaaat
     2101 gaatttgcgt ttttacatcc acataaaata acaacaaacg acttgaatgc taaacttaga
     2161 ctagcgttag caagcgatca aggtattact aaacatgata ttggactttc tgaacgcact
     2221 gtgtataaag tgtattttaa agacggatca tcaaaatttg ttgacttaaa agctgcgaaa
     2281 caagattcaa aagtatttaa agcaactgac attaaaaaag tagacattga aattaaattt
     2341 taatctttaa ttttagatta
//
LOCUS       DAKSQE010000194         2325 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.197, whole genome shotgun sequence.
ACCESSION   DAKSQE010000194 DAKSQE010000000
VERSION     DAKSQE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.197"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            282..724
                     /locus_tag="O0V33_002640"
                     /pseudo
     CDS             282..724
                     /locus_tag="O0V33_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
     gene            724..1167
                     /locus_tag="O0V33_002641"
     CDS             724..1167
                     /locus_tag="O0V33_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCX8977220.1"
                     /translation="MKKKYILILLIVILIGLLVIAYTHNKKTKDHYIETQEKRINLYF
                     KYNLNNYSSMKVTSFKKTPMGGYIVDGYVNHNKNYDFKVLISVTDNHQFEDSIGYDDK
                     TFGKLFKEKDHKNELKPKDIIKKEHLDKSEYEAEPPLFFFSGRLE"
     gene            1167..1610
                     /locus_tag="O0V33_002642"
     CDS             1167..1610
                     /locus_tag="O0V33_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCX8977221.1"
                     /translation="MNKKYILIIVSVILIGMIVISYAHNKQKKDHYIETQEKRIDLFF
                     KYNLNHYKSLKVTRFKKSPMDSYFIGGYINNNKDYEFQAFINVGGRKQFQRDIGYDEE
                     KLGKLLKEQEVKTWKTVDQIIKNEHLDKSEYEAEPPLFFFSGSLD"
     gene            complement(1785..1886)
                     /locus_tag="O0V33_002643"
     CDS             complement(1785..1886)
                     /locus_tag="O0V33_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977222.1"
                     /translation="MQGFKEKHQELKSALCQIGLMRSISEVRQLNIA"
     gene            complement(2009..2104)
                     /gene="pepA1"
                     /locus_tag="O0V33_002644"
     CDS             complement(2009..2104)
                     /gene="pepA1"
                     /locus_tag="O0V33_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin
                     PepA1"
                     /protein_id="HCX8977223.1"
                     /translation="MMLIFVHIIAPVISGCAIAFFSYWLSRRNTK"
BASE COUNT          941 a          283 c          387 g          714 t
ORIGIN      
        1 aatgttagaa aagagacaaa ttttgattta agtaataaaa tgataagtga acttaaaaag
       61 atattaattt agaataagat tgctatttta tacataatac gaaaagcaaa tcagcgcgac
      121 tttatgtatt taatactatg tcaagcaagt aatttttaaa aggtattttg aaacaacgaa
      181 tcatgagagg cgttgaacat agaaaaagat agcatgtcaa agtaaattgg tttttgaaat
      241 ctaaaatcta taagaaataa atactacact aggggattaa aatgaaaaag agatatgtat
      301 ttgtggtagt tgttgcaata ttaataggcc taattattat tgcatatgcg cataataagc
      361 aaaaaaagac cattacattg agacacaaga aaagcggata gatttgtatt ttaagcataa
      421 tcttaacaat tataaaaata tgaaggtgac agatttccat aaaactccga tgggtgggta
      481 tttcattata ggttatataa atgatgataa aaaatataaa ttccaagcaa gtatagatcc
      541 gggaagcgat aatcaatatc agaaagatat tggttaccat gaagataaat taggcaaatt
      601 attcaaagaa aaagatccta aatataaact ctctgtcgat gaaataattg aaaaagaaca
      661 cttagacaaa aatgaatatg aagcggaatc acctttattc ttcttttcgg ggagattaga
      721 gtgatgaaaa agaaatatat tttaatatta cttatagtaa ttttaatagg actgctagtc
      781 attgcttata cgcacaataa gaaaactaaa gaccattaca ttgagacgca agaaaaacga
      841 ataaatttat attttaagta taaccttaat aattatagct ctatgaaagt tactagtttt
      901 aaaaagacgc ctatgggtgg ctatattgtg gatggatatg ttaatcacaa caaaaattat
      961 gattttaaag ttctcatctc tgtaactgac aatcatcaat ttgaagatag cataggatac
     1021 gatgataaga catttggtaa attatttaaa gaaaaagacc ataaaaatga attaaaacct
     1081 aaagatatta taaaaaaaga acacttggat aaaagtgaat atgaagcaga accaccactg
     1141 ttcttctttt cagggagatt agagtgatga ataagaaata tattttaata atagttagtg
     1201 taattttaat aggtatgata gttatttcat atgcacataa taaacaaaaa aaagaccatt
     1261 acattgaaac acaagaaaag agaatagatt tattttttaa gtataacctg aatcattata
     1321 agtcacttaa agtaacgaga tttaaaaagt cgccaatgga cagttatttt ataggtggtt
     1381 atataaataa caataaagac tatgaattcc aagcttttat taatgtaggt ggtagaaagc
     1441 aatttcaaag agatatagga tatgacgaag aaaaacttgg aaaactatta aaagagcaag
     1501 aagtaaaaac ttggaaaacc gtcgatcaaa taattaaaaa tgaacactta gataaaagtg
     1561 aatatgaagc ggaaccaccg ttgtttttct tttcaggatc actggattag attcggttgt
     1621 atggtgtaat aaaaattatt attatcctca caatcattaa aggacaagag tattttaaaa
     1681 aatcattaca taacatacaa attttggtta tggaagttac aatcatttgc gcaatcgaat
     1741 attattatgt ttaaaacttt acgctccaaa aagtaaaaag gaagttaagc aatgtttagt
     1801 tgcctaactt ccgatattga acgcatcagt ccaatttggc atagagcact ctttagttct
     1861 tgatgtttct ctttaaaacc ttgcatattt tacaaaaaga aaaattagca gtataattga
     1921 gataacgaaa ataagtattt acttatacac caatcccctc actatttgcg gtagtgaggg
     1981 gatttttatt ggtgcggcta tatgtcacct attttgtatt gcgtctactt agccaataag
     2041 aaaaaaacgc aatggcacag ccactgatga ctggtgctat gatgtgaacg aaaataagca
     2101 tcaccttata cacctcctct ctgcgtctaa attgacgcct gagagatagg cgactctact
     2161 attatatcat ctaattggac aaattctatg atagtagatt ttgttaattt aagaaagaag
     2221 aacatttagt tcagagttta agttataaat ggttagatta tatgaaaatg gtaagccaag
     2281 gaatgaaatt atacgcgagt atgatttaac atctttgatg ttagg
//
LOCUS       DAKSQE010000195         2237 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.198, whole genome shotgun sequence.
ACCESSION   DAKSQE010000195 DAKSQE010000000
VERSION     DAKSQE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.198"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            115..1140
                     /gene="emp"
                     /locus_tag="O0V33_002645"
     CDS             115..1140
                     /gene="emp"
                     /locus_tag="O0V33_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HCX8977224.1"
                     /translation="MKKKLLVLTMSTLFATQFMNSNHANASTESVDKNFVVPESGINK
                     IIPTYDEFKKAPKVNVSNLADNKNFVASEDKLNKIADPSAASKIVDKNFAVPESKLGI
                     IVPEYKEINNRVNVTTNNPASKQVDKQIVAKDPEVNRFITQNKVNHRFITTQTHYKKV
                     ITSYKSTHVHKHVNHATSSIHHHFTIKPSEAPRYTHPSQSQSLIINHHFAVPGYHGHK
                     VVTPGQASIRIHHFCAVPQINSFKVIPSYGHNSHRMHVPSFQNNTTATHQNAKVNKTY
                     NYKYFYTYKVVKGVKKHFSFSKSHGCKIVKPALNIKNVNYQYAVPSNSPTHVVPEFQG
                     ILPAPRV"
     gene            1471..2028
                     /locus_tag="O0V33_002646"
     CDS             1471..2028
                     /locus_tag="O0V33_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001627793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977225.1"
                     /translation="MKRKVLVLTMGVLCATQLWQTNNAKALVTESGVNDTKQFTEVTS
                     EEKVIKDAISKVNESFIYYPQNDLKGLGGEHNDYEKITYSTSSNNVLELSMSSKYVGG
                     KSGAMVGYSEIYSSHFTDRDKRAIRRDHVKEAQNLINDYKYTQIYEDFAKATAKVSTL
                     SQSHQNYLNKQIDKVNNKIEKTEKR"
BASE COUNT          893 a          341 c          325 g          678 t
ORIGIN      
        1 tttacttcaa gttaattatt atactgaaaa ttctggtata taatgctgtt agtgaatata
       61 acaggaaaat taaattggtt atgatattga gtctatataa aggagaaata acagatgaaa
      121 aagaaattat tagttttaac tatgagcacg ctatttgcta cacaatttat gaattcaaat
      181 cacgctaatg catcaacaga aagtgttgat aaaaactttg tagttccaga atcgggtatt
      241 aataaaatta ttccaactta cgatgaattt aaaaaagcac caaaagtaaa tgttagtaat
      301 ttagctgaca acaaaaactt tgtagcttct gaagataaat tgaataagat tgcagatcca
      361 tcggcagcta gtaaaattgt agataaaaac tttgccgtac cagaatcaaa attaggaatc
      421 attgtaccag agtataaaga aatcaataat cgagtgaatg taacaacaaa caatccagct
      481 tcaaaacaag ttgacaagca aattgttgct aaagacccag aggtgaatag atttattacg
      541 caaaataaag taaaccatcg tttcattact acgcaaaccc actataagaa agttattact
      601 tcatacaaat caacacatgt acataaacat gtaaaccatg caacatcttc tatccatcat
      661 cactttacta ttaaaccatc agaagcacct agatatacac acccatctca atctcaatcg
      721 ttaattataa atcatcattt tgcagttcct ggataccatg gtcataaagt tgtaacacca
      781 ggacaagcta gtattagaat tcatcacttt tgtgctgtac ctcaaataaa tagttttaag
      841 gtcattccat catatggtca caattcacat cgtatgcatg taccaagttt ccaaaataac
      901 acaacagcaa cacatcaaaa tgcaaaagta aataaaactt ataactataa atatttttat
      961 acttataaag tagtcaaagg tgtaaaaaaa catttctcat tttcaaaatc acatggttgt
     1021 aaaattgtta aaccagcatt aaacatcaaa aatgtaaatt atcaatatgc tgttccaagt
     1081 aatagcccta cacacgttgt tcctgagttt cagggtatct taccagcacc acgagtataa
     1141 aaattgacat taagtttacg agatatgata aatacctatt attttaaaca tagtctgcaa
     1201 tctatgaggt tgtaggctat gttttttgca gtttatcaat aaacacccat caacaaatta
     1261 taccgttttt ctactttaaa agttggaagt aacataatct taaataaata tattattaat
     1321 taagataaat ataagactcg agattattgt taatagtttg ttcatcgcaa gttaattatt
     1381 gtttctaaaa tattggtata taattttcaa tggcgaagaa aacagggtaa aaaagtcggt
     1441 ttttaaatca aagcaaataa ggagtaaaaa atgaaaagga aagtactagt attaacaatg
     1501 ggcgtacttt gtgcgacaca attatggcaa acgaataatg caaaagcttt agtgacagag
     1561 agtggcgtta atgatactaa gcaatttact gaagtaacat cggaagaaaa agttataaaa
     1621 gatgctattt cgaaagtcaa tgaaagcttt atttactatc cccaaaatga tttgaaggga
     1681 ttaggtggag aacacaacga ttacgaaaaa attacatata gcacttcttc taataatgtt
     1741 ttagaattat caatgagttc aaaatacgta ggcggtaaat caggagctat ggttggttat
     1801 agtgaaattt actcatcaca tttcacagac cgcgacaaac gtgctatcag acgtgatcat
     1861 gttaaagaag cacaaaactt gattaatgat tataaatata cgcaaatata tgaagacttt
     1921 gctaaagcta ctgcaaaggt aagtacactt agtcagtctc accaaaatta tttaaataaa
     1981 caaattgata aagtgaataa taagatagag aaaactgaaa aacgctaata caaagtaatt
     2041 tataagttat acatttcgtt tttaaatgac aatttatccc cgtaaatata ataaataatc
     2101 ttttcaaatt ccgcatagat atagagacgc taataaacct ctttgtctcg atatgatagt
     2161 ctgcaacgat tcatgttgta ggctttttaa ttttacaaat acgactaaat atataagttc
     2221 tgacacctaa aatatag
//
LOCUS       DAKSQE010000196         2224 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.199, whole genome shotgun sequence.
ACCESSION   DAKSQE010000196 DAKSQE010000000
VERSION     DAKSQE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.199"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            55..305
                     /locus_tag="O0V33_002647"
                     /pseudo
     CDS             55..305
                     /locus_tag="O0V33_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187510.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            627..806
                     /locus_tag="O0V33_002648"
     CDS             627..806
                     /locus_tag="O0V33_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977226.1"
                     /translation="MKFKTTKECKSNNIFKRSQEINNRESEKGCLWGISMLILLFLLI
                     LFGITACSSSIHFIN"
     gene            1117..1377
                     /locus_tag="O0V33_002649"
     CDS             1117..1377
                     /locus_tag="O0V33_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977227.1"
                     /translation="MCIVIYLIYSLIVYFICIINFFIMAAPFLMTNADYVWTPMTTVT
                     LFILSLIIFLIFIKTKDVVHLTIFILNLLFSILYCLPILFYL"
     gene            complement(1430..1780)
                     /gene="scn"
                     /locus_tag="O0V33_002650"
     CDS             complement(1430..1780)
                     /gene="scn"
                     /locus_tag="O0V33_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-A"
                     /protein_id="HCX8977228.1"
                     /translation="MKIRKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNE
                     KLANELKSLLDELNVNELATGSLNTYYKRTIKISGQKAMYALKSKDFKKMSEAKYQLQ
                     KIYNEIDEALKSKY"
BASE COUNT          797 a          291 c          292 g          844 t
ORIGIN      
        1 ttagttaatc aattttgcat ctattttgtt gtaagctata taaaaggagt gataatgatg
       61 gtgaaaaaaa caaaatccaa cacactaaaa aaagatgcaa cacttgcatt agcaaattta
      121 ttattaattg gggcactgac agacaatagt gccaaagccg aatctaagaa agatgatact
      181 gatttgaagt tagttagtca taacgtttat atgttatcga ccgttttgta tccaaactgg
      241 agacttttaa cataaaatta cttatcattc aaaaagtaaa acagcataat atcaaggttt
      301 ataactttat cattatcaat aatacctcat ataaaataaa attttaggga ctttttaggg
      361 actttaaatt taaaattaca agtttaatag aaacatcaaa ataatcacat gtttgtgtgg
      421 aatgtacacc ccaaaagcta gactgaaaaa tctatttttt gaggtgtatt tttataggta
      481 aatataataa attagagtag acaactcaga attccaattt tataataata ttgcttgaca
      541 tatcaaacta gatagtacta ttttgaatat attattataa tcaaaaattc attgtaaact
      601 tttagacaaa aggaagtaat aaaaacgtga aatttaaaac aactaaagaa tgtaaaagta
      661 ataatatctt taaaagaagt caagaaatta ataatagaga aagtgaaaag ggttgtttat
      721 ggggcattag catgttgatt ctacttttct tattgattct gtttggaata actgcttgtt
      781 catcaagcat tcactttatt aattagattt ttttacttgg aggtatcatg tgaagaacca
      841 tacaaatata attaatatct tattagttat agtcaactca ttaactcatt ttctaactct
      901 aaacacctca ttttttaata attcagcatc ggatttctgt tttatcatag gggctatatt
      961 tttcttaatc ggaatttttg ttgcaatata cggtatgaag cgagcaacat attggttaaa
     1021 cttattgatt ttatttacca atatttttta ttttctacac ttctgtgtgt tacttttgtt
     1081 aaaatatata ggatttaaat tatttattta tgaagggtgt gtattgttat ttacctaatt
     1141 tatagtctaa ttgtctattt catctgcatt ataaattttt tcataatggc tgcaccattc
     1201 ttaatgacta atgctgatta tgtatggacg cctatgacta cagttacgtt atttattttg
     1261 agtttgatta ttttcttgat atttataaaa acaaaagatg tcgttcattt aacaattttc
     1321 atattaaact tacttttttc aatactttat tgtttgccta tactgtttta tttatgaaca
     1381 cttttatatt atctagaaaa ttaaaaccac ccttttacgg gtggtttttt taatatttac
     1441 tttttagtgc ttcgtcaatt tcgttataaa tcttttgaag ttgatatttt gcttctgaca
     1501 ttttcttaaa gtcttttgac ttaagagcat acattgcttt ttgacctgaa atttttatag
     1561 ttcgcttata ataagtgttt aaacttccag tagctaattc attaacattt agttcatcta
     1621 ataacgattt taattcatta gctaactttt cgttttgata ttcattcgat gttggcaagc
     1681 ttgtgctagc ttgtgcctca tttttatcta gattagttac tagtggtgat gctaaaacga
     1741 ttgctaaagt tcccgcaagt atagattttc taattttcat tttttattct ccttagtaat
     1801 tagtactaat ttattttaat gactaactaa aaaaaagtac attaattatt cattaactat
     1861 atagatcttc ttattaaatg aaaattaata aattatcaaa ttgacatacc ttattgtatt
     1921 tatagaaaaa caaaaaaagg taagcaccga aatgcttacc tacttcccat aaacaatata
     1981 acacatatac attgatttgg aaagcgcaaa aataaattta ataaactagc ccgaagggga
     2041 gcaatacata aaaaatgaaa ggcgctcctt gaaaacgccc aaggtaatat taacataaaa
     2101 tggctactat tgcattatct aattttattt aattaaaaca aatatatata gaacaaaaaa
     2161 actagcccga aggctagctt atgaatagat gaaaatttga acacattgct gtgtctaaga
     2221 taaa
//
LOCUS       DAKSQE010000197         2212 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.200, whole genome shotgun sequence.
ACCESSION   DAKSQE010000197 DAKSQE010000000
VERSION     DAKSQE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.200"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            1..116
                     /locus_tag="O0V33_002651"
                     /pseudo
     CDS             1..116
                     /locus_tag="O0V33_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451275.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
     gene            123..194
                     /locus_tag="O0V33_002652"
                     /pseudo
     CDS             123..194
                     /locus_tag="O0V33_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            201..398
                     /locus_tag="O0V33_002653"
     CDS             201..398
                     /locus_tag="O0V33_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain protein"
                     /protein_id="HCX8977229.1"
                     /translation="MNLQSYIGKLVKLTLTNNKILIGKVIDFDDKIDNFDGYNSIEID
                     TGRITYDISENKIKDIQIITK"
     gene            complement(945..1970)
                     /locus_tag="O0V33_002654"
     CDS             complement(945..1970)
                     /locus_tag="O0V33_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HCX8977230.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFIN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
BASE COUNT          794 a          382 c          284 g          752 t
ORIGIN      
        1 caggtgtaaa tgcgtcacct atgcatcctt ggcgtagaag taccacagtg ccacatctcg
       61 gcaattggcg agacaagttc tttaaagagc gtgaaggtaa atatcgggta gagaacaata
      121 ctgaaatgaa attaggtgaa gttcattatt ctaaaactgg ttttcatgta gttccgtata
      181 ttaaaaagga gtgattaaaa atgaatttac agtcatacat aggcaaatta gtcaaattaa
      241 cgcttactaa taataaaata ttaattggaa aagtgataga ctttgatgat aaaattgata
      301 attttgatgg ttataattcg atagaaattg atacaggtag aattacatat gatatatcag
      361 aaaataaaat taaagacata caaataatta caaaatagca tcctttctac acagataaag
      421 agaaagcggt gctattttta tacacttttt taacaactaa ggatacaaga cacatacctt
      481 caacaatatc ctcatctaaa cctatcgatt tcctaagctc aattttaaac acaaagttta
      541 tttgtaaaga tgcacaccta taaaaatctt aatcatcgaa aagtcacaaa catctccata
      601 ttaaaaactt aaatagtgca ttccatttaa actaaacatc gatatttcta ccaatataga
      661 cttagtaatt cgattgttaa tacattactt tcataaaata actacaacca tcataatact
      721 tctcaaatct cgtcaaatta aagtaaatac agcacaaatt aacaatttta aatatataaa
      781 ataaaacaag atactcaata atctgtagta ccttgttttg gaataatgta attttatatc
      841 atcaacggct acaaatgagc agtttcatat ataaataaac acaactactc aactgatctt
      901 gattaattaa taaatgattt gccagatgac atataacagc attattagat taattctaat
      961 agtttattta aattttcttc ggttgtcgcc caactggttg cgaatctaac aacacgatgt
     1021 tgatcatcgt atttttccca aacagcaaat ttaacttttt gttctaactc tgctattttc
     1081 tcgttactta aaataaaaaa ttgttgattg gttggagaat caaagtaaag acgatagcct
     1141 ttatttataa acccgtcttt catcttattt gccatttcga tagcatgtct gcttatatta
     1201 aaatataaat tatccgtaaa taattctaaa aattgtatgc ctgttaaccg tccttttgct
     1261 aaaagtgcac cgtgatgttt gattcgagtg gtaaattgtt tcggttcatt attcttcgta
     1321 aaaacaattg cttctccgca taatgcacct atcttcgtac cacctatata aaatacatca
     1381 caatatttag cgatgtcttt aatagtcata tctgattgat cactcatcaa tccataccct
     1441 aatcgtgcac catccataaa taatggaagc tgatattgct tacatacttt gcataactct
     1501 tccaattctg atttagagta taatgtgcca tattcagtag gatgagaaat atataccatt
     1561 cctgggaata ccatatggtc ctttttaaaa tcacttttaa atgtctccat gtaagtttca
     1621 acatctgaag cactaacttt tccttcctta gagggtatag taattacttt atgtccacta
     1681 tattcaattg caccgccctc atgcacagca acatgaccag tgtctgctga aatgacccct
     1741 tcgtaacttt ctaacattga attaataaca acctgattgg tttgtgttcc acctactaaa
     1801 aaacgaattg tagcatttgg acagtcaatt gtatctttaa tcttttcaat tgcctgagct
     1861 gtgaattgat caaagccata tcccgaagct tgtacaagat ttgtatctac taatcgtttt
     1921 aatacttttt catgagcacc ttctaaataa tcattttcga atgaaatcac tacatttccc
     1981 cctaaaacta atatcaacat tttaataaga taaaccaatt tcaaaactag ttcgatattt
     2041 aaaatgtatt atgaatggtt aaagtttgta tcgcattatc gcgaagttga ataaatatat
     2101 tatacatcaa acaatacatt atgaaaatta attaattttt taccacaatt actttgcgtg
     2161 ccgaaacaca tatattaatg attgaatttt actgttaaaa gttctatgat tt
//
LOCUS       DAKSQE010000198         2171 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.201, whole genome shotgun sequence.
ACCESSION   DAKSQE010000198 DAKSQE010000000
VERSION     DAKSQE010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.201"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            185..388
                     /locus_tag="O0V33_002655"
     CDS             185..388
                     /locus_tag="O0V33_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCX8977231.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            complement(385..621)
                     /locus_tag="O0V33_002656"
     CDS             complement(385..621)
                     /locus_tag="O0V33_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977232.1"
                     /translation="MDNHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            846..1427
                     /locus_tag="O0V33_002657"
     CDS             846..1427
                     /locus_tag="O0V33_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCX8977233.1"
                     /translation="MAKTLYLMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGLFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEDRIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            complement(1500..2117)
                     /locus_tag="O0V33_002658"
     CDS             complement(1500..2117)
                     /locus_tag="O0V33_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HCX8977234.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     TAGLSDSLFIIIAVVGVSIIIMSLPILQAIIYIVGLIFLMYMAWTIWHDKPSTDGEAQ
                     IMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGKMVGSIDKTGKLLTIINKISSIIIIIVALMILQKLIQLLF"
BASE COUNT          798 a          329 c          327 g          717 t
ORIGIN      
        1 attaatgtga aaagaatatt tacatactaa aaacccttaa agttgtacaa ttcgttaata
       61 tttttagaat cagataacaa aaaaatgtgc aaaatcactt ttgcacattt acaaatacta
      121 gttttctgat aagattaata ataacacttt aaatagcgtt taataaatga agggggcaag
      181 tcttatgacg ttttacaatt tcatcatggg ttttcaaaat gataacacac catttggtat
      241 attggccgaa cacgttagtg aagataaagc attccctcga ttagaagaaa gacaccaagt
      301 aattagagca tatgtgatgt ctaattacac agatcatcaa ttaattgaaa ctacaaatag
      361 agctattagc ttatatatgg caaattaatt tgagtagtac caattatgat gtattagtgc
      421 atcccaaata tcttttgttt taaagtttat ttcatcattt cttatcgaaa atggtgtaat
      481 aatgtcttta tctaaccaag tgtttataag ttcatttggt acaccatcta acaacatttc
      541 acttttacta attataaaac attcccagtc aagtgaaaca ttttgtggat tcacataatt
      601 acattgatta tgattatcca taaacactca ctcctttaaa attctgtact cttcattgcg
      661 ttttaccccg tcacattatc ttttaaacta aaatcatcat tacttatgaa aaaaatgttc
      721 atcaaaagca aaggttttcg ctaccgaaaa agtttaaata atggtttaat atatttggta
      781 ctcattttaa taaaaagaga atacattttg agctatcaat actttttatt gaagaggtgt
      841 tattgttggc taaaacgtta tatttaatgc gccacggaca aactttgttt aattttaagg
      901 gactaattca gggatttgga gattcgccgc taacagaact tggaattgct caagctcaaa
      961 aggcacgtag ctattatgaa actaagggga taaacttcga tttatatgca tcatcaacgc
     1021 aagaacgtgc aagtgacaca cttgaaaatg ttgcacctaa tcaatcgtat caacgtttta
     1081 agggactgaa ggaatggcat tttggattat ttgaaggtga gtcagtctat ctatttgata
     1141 atctatacaa gcctgaagac ttattcggag atcgaattgt tcctttcaaa ggagaggcaa
     1201 ggcaacaagt tgaagatcgc attgtgaaaa ctttacatga cattatgtct caaacaaaga
     1261 ataatgcatt agtcgtgagt catggaacaa taatgggagt atttttaaga tattgcctta
     1321 aactagatga agcattaaag cataatatcg gtaattgtaa tatcttgaaa tttgaatatg
     1381 acaatggaac atttaaattt gttgagttaa ttgatccaaa tttataataa ataaagcgtt
     1441 atacatagac acaagtatgt cttttgtata acgcttttaa aatacaacta catgacgctt
     1501 tagaataata attgaataag tttttgtaat atcatcagag caacaataat aattattatg
     1561 cttgatattt tattgattat tgttaataat tttccggttt tatcaattga tccaaccatt
     1621 tttcctaaaa ttgcgagtaa aaagaaccat aaccatgaaa cgctaataca agcgattgta
     1681 aacgcaattt tattgctgcc actatataat gcagcactac taccaattac accaattgta
     1741 tctaaaatag catgtggatt gagtaatgaa actgataaag caaaacttac ttgtttcatt
     1801 ggagacataa tttgagcttc tccatctgtt gagggtttat catgccaaat ggtccaagcc
     1861 atatacatca agaaaattaa accaactata taaataattg cttgaagtat aggtaaagac
     1921 ataataatga tagaaactcc taccactgca ataataataa ataagctgtc tgacaacccg
     1981 gctgtaatta ttgcaggcaa tacatatcta tattttggtt gattagctcc ttggttaaaa
     2041 ataaatacat tttgtgcacc taaaggtaaa attagaccaa tcgctaagat aaatccatga
     2101 ataattgcgg ttaccattaa gttttgacac ccctaaaaca tagaatattc tgaaaaaact
     2161 tattgatatc a
//
LOCUS       DAKSQE010000199         2117 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.202, whole genome shotgun sequence.
ACCESSION   DAKSQE010000199 DAKSQE010000000
VERSION     DAKSQE010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.202"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(413..2059)
                     /locus_tag="O0V33_002659"
     CDS             complement(413..2059)
                     /locus_tag="O0V33_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCX8977235.1"
                     /translation="MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTN
                     NIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFS
                     AGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
                     LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQIN
                     GGLHYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAAL
                     IVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPF
                     VGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKYKEIFDMTPETQ
                     LFGVFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSRMVKVVWGI
                     SQALIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLG
                     LTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
BASE COUNT          765 a          392 c          306 g          654 t
ORIGIN      
        1 aggtgtaata agtatttttt attaattgag aataattatc aatttcgtga atgattcaat
       61 tcaattttta aacgtattat ttcattgagc agaaagaaaa ttattgcgtc aaactttaaa
      121 atttcttttc aatgtcattc ttttgatata cacataccga aatatgttta gttaattttt
      181 gtttataccg tcgtacatca ctttagcttt gtcaaacccc ttactattaa gttgtcataa
      241 ataaacctat tttaaaatat aaaaaaatcg agaattcgta gtttaataac gaaattctcg
      301 ttcttatcat tttgaatata ctcaattttc cacaaaaata aacaagtagt atatctgttc
      361 tagctactag aatgacatac tacttgttat taaaatactt gactaaattt tattagttat
      421 cttttttctc tatatttcta cgtgactgac gcttttcaag aatgtcagat tcataatctt
      481 cttgttgact cttgatatat tcttgtaagc ggtgtttatt cggagtcaat gttaaaccta
      541 ggaatttacg ttcctggttt gcatccttgt agaaacttac catcatgagt atgacgacaa
      601 aggagaatgg gaatgcactt ataattgcag cactttgaat cgcatttaaa gcttcagcac
      661 cgttaccgcc accagctaat aaaagtacaa atgctattaa ggcctgtgaa attccccaaa
      721 caacttttac cattctagat ggatttaatg aaccaaatgt tgtttgcatt cctaatacaa
      781 atgttgctga gtcagcagat gtaataaaga atgatgcaat taataataat gcaatcaacg
      841 ataaaacaat gccaaatggc acatgattaa acactccaaa tagctgtgtt tcaggagtca
      901 tatcaaaaat ttctttgtat ttcttacctg tctcgatgcc taatacacca aagacactaa
      961 accatacaaa actaacaatt gctggaacta gcaagacacc agaaatgaac tctctaattg
     1021 aacgtccttt tgaaactcgt gcaataaaca caccaacgaa tgggctccaa cttaaccacc
     1081 aaccccagta ataaagtgtc catgaagaca tccattcacg tttttgagga tttaaagcag
     1141 ctgtatcaaa actattaaac aagaatgtgt ttagtaaact acccgtagag ctagttaaca
     1201 tatttaaaat aagaacagtt ggtccaacaa ttaaagcagc taccattaaa atagtaccta
     1261 aaccaatgtt caagttactt aagtattgaa tacctttact taatccagac catgcacttg
     1321 ctataaataa gatagtaaca acaatgatga taatcgcttg tacaaacgta ttgtttggaa
     1381 cattgaacaa gtaatgtaaa ccaccattaa tttgtagagc acccatacct aacgaaacag
     1441 ctaccccaac gattgtcgca aatacagata aaacgtcaat aaaaatccca ataggacctt
     1501 ctactttatc acctaaaaga ggacgtaaag ttctagataa taaacctggt tcacctttac
     1561 ggaattgcga atatgccaac gctaacgcaa caacaccata aacagcccaa gcatggaatc
     1621 cccaatggaa aaatgttgaa cgtagggctt cagtataagc ttcagtagtt ttgggatctg
     1681 ccgtaggtgg cgtagcaaag tgcgccatcg gttcagctgc accataaaac accaaaccta
     1741 tccccatacc agcactaaac aacatagcaa accatgaaat tgtattaaac tcaggtttgt
     1801 catttggttt acctagttta agttttccaa taggactaaa aataaggaat atacagaaga
     1861 acacgataat cgtagtaaga ataagataat accaacctaa cttttctgta atccacattt
     1921 taatattatt ggtaacatag ttgaattgtt caggtaaaaa tgcaccaagt aatacgacta
     1981 tagcaacaac aattgcacta tagatgaaga ctggtgaata cttctttcca tttggattct
     2041 ctggtgaaga agaattcata attaattact cccttcaatt ctatatttaa ttttatgtag
     2101 tagaataaaa atattat
//
LOCUS       DAKSQE010000200         2107 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.203, whole genome shotgun sequence.
ACCESSION   DAKSQE010000200 DAKSQE010000000
VERSION     DAKSQE010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.203"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            177..>2107
                     /gene="ltaS"
                     /locus_tag="O0V33_002660"
     CDS             177..>2107
                     /gene="ltaS"
                     /locus_tag="O0V33_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HCX8977236.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANS"
BASE COUNT          783 a          309 c          335 g          680 t
ORIGIN      
        1 atcaaagttg aaagctacaa ataatgaaaa ttataaactt gaatctgaaa gtaattacta
       61 taattatgac aatgttaact tttaaacgca cttattaatt aactacataa tgttaatatc
      121 taatttattc aagtactttc gcaagattta ttatctaaat aacgggggaa agaatcatga
      181 gttcacaaaa aaagaaaatt agtctttttg cgttcttctt attaaccgta ataacgatta
      241 ccttgaagac gtatttttct tattatgttg atttttcttt aggtgttaaa ggtttagtac
      301 aaaacttaat attattgatg aatccttata gtttagtagc actggtttta agtgtgttcc
      361 tattctttaa aggcaaaaaa gcattttggt ttatgttcat aggcggcttc ttattgacgt
      421 tcctattata tgccaatgtt gtgtacttta gattcttctc ggatttttta acgtttagta
      481 ctttaaacca agtaggtaac gtagaatcta tgggtggtgc ggttagtgca tcattcaaat
      541 ggtatgactt tgtttatttc attgatacgt tagtttactt attcatttta atatttaaaa
      601 caaaatggtt agacacaaaa gcatttagta agaaatttgt tcctgtcgta atggcggctt
      661 cagtagcatt attcttctta aacttagctt ttgctgaaac tgacagacca gaattattaa
      721 cacgtacatt tgaccataaa tatttagtga aatatttagg accgtataac tttacagtat
      781 acgatggtgt taaaactatc gaaaataatc aacaaaaagc gttagcatct gaagatgact
      841 taacaaaagt attaaattat acgaaacaac gtcaaacaga gcctaaccca gaatattatg
      901 gggtagcaaa gaagaaaaat attattaaga ttcacttaga aagtttccaa accttcttaa
      961 ttaataaaaa ggttaatggt aaagaagtaa caccgttttt aaacaaatta tcaagtggga
     1021 aagagcaatt cacatacttc cctaactttt tccatcaaac aggtcaaggt aaaacatctg
     1081 actctgaatt tacaatggat aacagtttat acggtttacc gcaaggttct gccttttcat
     1141 taaaaggaga taatacgtat cagtcattac cagcaatttt agatcaaaag caaggctaca
     1201 aatctgatgt catgcacggt gactataaaa cattctggaa cagagaccaa gtatataaac
     1261 actttggtat cgataaattc tatgatgcaa catactatga catgtcagat aaaaacgttg
     1321 taaacttagg cttgaaagac aaaattttct ttaaagattc tgctaattat caagctaaga
     1381 tgaaatcacc attctattct catttaatta cattgactaa ccactatcca ttcacattag
     1441 atgaaaagga tgcaactatt gagaagtcaa acacaggtga tgcaacagtt gatggttata
     1501 ttcaaacagc acgttattta gacgaagcat tagaagaata tattaatgac ttgaagaaaa
     1561 aaggattata tgacaattca gtgattatga tttatggtga ccactatggt atctctgaaa
     1621 accataacaa tgccatggaa aaactattag gtgaaaaaat cacaccagct aaatttacag
     1681 atttaaacag aactggtttc tggattaaaa tccctggtaa atctggtggt atcaataatg
     1741 aatatgctgg tcaagtcgat gtaatgccaa caattttaca tttggctggt atagatacga
     1801 agaactattt aatgttcggt actgatttat tctctaaagg tcataatcaa gtagttccat
     1861 tcagaaatgg tgactttata acaaaagatt ataaatatgt taatggtaag atttattcta
     1921 ataaaaataa tgaactcata actactcaac cagctgattt cgaaaagaat aaaaagcaag
     1981 ttgaaaagga tctcgaaatg agtgacaacg tgcttaatgg tgatttattt agattctaca
     2041 aaaatccaga cttcaaaaag gtaaatcctt cgaagtataa atatgaaaca ggacctaaag
     2101 caaactc
//
LOCUS       DAKSQE010000201         2068 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.204, whole genome shotgun sequence.
ACCESSION   DAKSQE010000201 DAKSQE010000000
VERSION     DAKSQE010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.204"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            58..345
                     /locus_tag="O0V33_002661"
     CDS             58..345
                     /locus_tag="O0V33_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977237.1"
                     /translation="MAKEIINNTERFILVQIDKEGTERVVYQDFTGSFTTSEMVNHAQ
                     DFKSEENAKKIAETLNLLYQLTNKKQRVKVVKEVVDRTDLSSDKTVDSETM"
     gene            401..775
                     /locus_tag="O0V33_002662"
     CDS             401..775
                     /locus_tag="O0V33_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977238.1"
                     /translation="MDDIQKIKKELSELVERVDDVEILANETADHVLELREEHKQHHN
                     ELRESHKELKDKQDKVVDENLEQTKILNRIEERYQTQVDVAQKNEEKTLAQNKWLVGA
                     IWALVTIVMIAVITASITALLP"
     gene            1196..1969
                     /gene="sea"
                     /locus_tag="O0V33_002663"
     CDS             1196..1969
                     /gene="sea"
                     /locus_tag="O0V33_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type A"
                     /protein_id="HCX8977239.1"
                     /translation="MKKTAFILLLFIALTWTTSPLVNGSEKSEEINEKDLRKKSELQG
                     AALGNLKQIYYYNEKAKTENKESHDQFLQHTILFKGFFTNHSWYNDLLVDFDSKDIVD
                     KYKGKKVDLYGAYYGYQCAGGTPNKTACMYGGVTLHDNNRLTEEKKVPINLWLDGKQN
                     TVPLETVKTNKKNVTVQELDLQARRYLQEKYNLYNSDVFDGKVQRGLIVFHTSTEPSV
                     NYDLFGAQGQNSNTLLRIYRDNKTINSENMHIDIYLYTS"
BASE COUNT          815 a          247 c          392 g          614 t
ORIGIN      
        1 aggaagagta atccttagca ctatttttat acaaaaattt aaggaggtca tttaattatg
       61 gcaaaagaaa ttatcaacaa tacagaaagg tttattttag tacaaatcga caaagaaggt
      121 acagaacgtg tagtatatca agatttcaca ggaagtttta caacttctga aatggttaac
      181 catgctcaag attttaaatc tgaagaaaac gctaagaaaa ttgcggagac gttaaatttg
      241 ttatatcaat taactaacaa aaaacaacgt gtgaaagtag tgaaagaagt tgtggataga
      301 actgacttgt catctgataa aacagttgat agcgaaacaa tgtagctata ctaagctatg
      361 agcattacgc tcatagcttt cttagaaagt aggtgtagtt ttggatgata ttcagaaaat
      421 aaaaaaagag ctttctgaat tagttgaacg tgtggatgat gttgaaatac tagcaaatga
      481 aacagctgat catgtgcttg aacttagaga ggaacataag caacatcata atgaactaag
      541 agaatctcat aaagaactta aagataagca agataaagtt gtagatgaga atttagagca
      601 aacaaagata ttaaacagaa ttgaagaaag atatcaaacg caagtagatg ttgcgcaaaa
      661 aaacgaagaa aagacactcg cccaaaataa atggctcgta ggtgccatat gggcgcttgt
      721 aacaattgtt atgattgcag tcattactgc atcaattact gcgttattac cttaagggag
      781 gtggacaaaa tgagttgggc aagatggttg tcatgttatt tgtatggtcg taaatgtaaa
      841 taatgttttt ggtcagtgct tcggcactgg ctttttattt tgattgaaaa gagttacgta
      901 cattgtatta cacagctcaa aagacaggaa gcatactgca agtgaagttg ggaagtgttg
      961 tcaataccaa gtaagtaaga tatctgaaat gtataataga gtaaaaatga aatcttttta
     1021 ttatattata gacaagtata aaaaaggtat agtaatatat gtatgtataa gtaaataatg
     1081 ataattctat aattattgta tataactaat aattacttcg acaaaaataa tctattatcc
     1141 aaatatttta gataataaaa agtttgtatg gaattatgct ttagaggtga gcaaaatgaa
     1201 aaaaacagca tttatactac ttttattcat tgccctaacg tggacaacaa gtccacttgt
     1261 aaatggtagc gagaaaagcg aagaaataaa tgaaaaagat ttgcgaaaaa agtctgaatt
     1321 gcagggagca gctttaggca atcttaaaca aatctattat tacaatgaaa aagctaaaac
     1381 tgaaaataaa gagagtcacg atcaattttt acagcatact atattgttta aaggcttttt
     1441 tacaaatcat tcatggtata acgatttatt agtagatttt gattcaaagg atattgttga
     1501 taaatataaa gggaaaaaag tagacttata tggtgcttat tatggttatc aatgtgcggg
     1561 tggtacacca aacaaaacag cttgcatgta tggtggtgta acgttacatg ataataatcg
     1621 attgaccgaa gagaaaaaag tgccaatcaa tttatggcta gacggtaaac aaaatacagt
     1681 acctttggaa acggttaaaa cgaataagaa aaatgtaact gttcaggagt tggatcttca
     1741 agcaagacgt tatttacagg aaaaatataa tttatataac tctgatgttt ttgatgggaa
     1801 ggttcagagg ggattaatcg tgtttcatac ttctacagaa ccttcggtta attacgattt
     1861 atttggtgct caaggacaga attcaaatac actattaaga atatatagag ataataaaac
     1921 gattaactct gaaaacatgc atattgatat atatttatat acaagttaaa catggtagtt
     1981 ttgaacacgt aatgttcaga ttattatgaa ccgagaataa tctgaaagtt tacaagcagt
     2041 aaaaaaagta tatgtgctat aatatgct
//
LOCUS       DAKSQE010000202         2035 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.205, whole genome shotgun sequence.
ACCESSION   DAKSQE010000202 DAKSQE010000000
VERSION     DAKSQE010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.205"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(128..1135)
                     /locus_tag="O0V33_002664"
     CDS             complement(128..1135)
                     /locus_tag="O0V33_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HCX8977240.1"
                     /translation="MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKT
                     AILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYG
                     IPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
                     VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVE
                     PQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLH
                     FPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLF
                     F"
     gene            complement(1151..1768)
                     /locus_tag="O0V33_002665"
     CDS             complement(1151..1768)
                     /locus_tag="O0V33_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HCX8977241.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETIIMKNS"
BASE COUNT          701 a          365 c          286 g          683 t
ORIGIN      
        1 ttaaaatgct ttgtcttggt cattctatgt aaactctata aaataggttg tctatttaca
       61 taaaaaaatg aacttcaata ccaccatata tttgtgatac tgaagttcag tatagcttaa
      121 ttttcaatta gaaaaataaa ttaagtatat agaataataa acctgctaac aatgctgaaa
      181 taggtaatgt aatcacccat gtaatgatca ttcgttgcgc agtactccat tttacacctt
      241 tagctcggtt agaagcacca acacctaaga ttgatgatga cacaacgtga gttgttgata
      301 atgggaaatg tagcgatgat gcaacaaaaa ttgttaatgc agatgataaa tcggccgcag
      361 caccgtttgc tgggcgtatt ttcataatat taccacctac agttttgata attttccagc
      421 caccaattgc agtaccaagc cccattgctg tcgcacaggc aaattttacc cataactgtg
      481 gttcaacgct gccatcattc tgtacattag caacaatcaa tgccaacgta ataataccca
      541 ttgatttttg cgcatcattc gtaccgtgag agaatgattg taacgctgct gtgaaaattt
      601 ggaaaaatct aaagttacga ttcgctcttg ttaaatttgc atttttaaag ataactttaa
      661 aaattgaata catcaagaaa ccaacacaaa atgcgataat cggtgaaacg attaatacaa
      721 taataatttt tgtgaaacct tggtaatgta atacgccaaa tgagccttca gatgcgattg
      781 ctgcacccgc aattgaacct ataagtgcat gtgaagacga acttggaatt ccgtaaaacc
      841 aagtagctaa attccaaata atagccgcaa gtattgcagc taacacaaca actaatccat
      901 tttccaattt aaatggatcg acaatgtctt tagtaatggt gcctgcaacg cccgtaaatg
      961 ttaaagcacc tataaagttc atcactgctg ccattaaaat tgccgtttta ggcgttaacg
     1021 ctctagtaga tacagcagta gctactgcat tggctgtatc atggaatcca ttgataaagt
     1081 caaatatcag cgagaaaata actacagcta tagtgacgat gattatatat gacataaata
     1141 tatactcccc ttagctattt ttcataataa tagtttcaaa attatttgct acgatttgac
     1201 atttatcagc aatttcttcc atgctttcat aaatatcttt tattttaatt aatgtgattg
     1261 gatctgtttc actattgaaa atatgtttaa ttgactgtct taaaatacca tcacagtttg
     1321 tttcaaattc tttaatatta attgaatgaa tacgcatatg tgataatttt ttatcgacta
     1381 ataagccgac agcaagtttc atttctgcaa ctgctttttg aatgttatca acaaactcag
     1441 ccatatattc atctgtgtat tcgattgaat acatttcaaa catagctgcc gtttcttcaa
     1501 ttgcatctaa aacatcatca attgcatcac ataatgataa aatatcttca cgttcaattg
     1561 gtgtgataaa tgtttgattt aaatcagtaa ttacttgatg tactaattcg tcaccatgtg
     1621 actcataagt tttaatgttg tctgagtatg cctttaaatc tagatgtgta ttgaaatcca
     1681 ttttaccgaa ttcaatagct gcacgatcta ggttaaaaac catctcttct aattgaacca
     1741 taaacttatc ttttttctta ctaaacattt aaaatcctcc atttaagcga ttgtcaccaa
     1801 tcacattcag ttataatttg tttcaaatta agacaagtga atttacaaac taatgataca
     1861 aatttgttat tatcaatcgt cagtatattt ttagtgtact gatattaatt tcaaaaatgc
     1921 ctcacagtaa acaatttact gtatttgccc ttatatgaaa atagcacaaa atttacaatc
     1981 ccacaattca atttacaaat ttttaacaat tccgaaactt aacttttaca taaaa
//
LOCUS       DAKSQE010000203         2023 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.206, whole genome shotgun sequence.
ACCESSION   DAKSQE010000203 DAKSQE010000000
VERSION     DAKSQE010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.206"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            126..>2023
                     /gene="clfB"
                     /locus_tag="O0V33_002666"
     CDS             126..>2023
                     /gene="clfB"
                     /locus_tag="O0V33_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HCX8977242.1"
                     /translation="MDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQASEQS
                     NDTTQSSKNNASADSEKNNTIETPQLNTTANDTSDISANTNSANVDSTAKPMSTQTSN
                     TTTTEPASTNETPQPTAIKDQATAAKMQDQTVPQEANSQVDNKTTNDANSIATNSELK
                     NPQTLDLPQSSPQTISNAQGTSEPSVRTRAVRSLAVAEPVVNAADAKGTNVNDKVTAS
                     DFKLEKTTFDPNQSGNTFMAANFKVAGKVKSGDYFTAKLPDSVTGNGDVDYSNSNNTM
                     PIADIVNDKKEVVAKATYDILTKTYTFVFTDYVNDKENINGQFSLPLFTDRAKAPKSG
                     TYDANINIADEMFNNQITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQTVFV
                     NPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDTSKLSDSYYADPNDSN
                     LKEVTNEFKDKITYKYQNVASINFGDINKTYVVLVEGHYDNTGKNLKTQVIQENIDPA
                     TGKDYSIFGWNNENVVRYGGGSADGDSAVNPVDPTPGPPVDPEPEPEPTPDPEPSPEP
                     EPEPEPTPDPEPSPDPDPEPTPDPEPSPEPDPDSDSDSDSGSDSDSGSDSDSDSDSDS
                     DSDSDSDS"
BASE COUNT          777 a          362 c          390 g          494 t
ORIGIN      
        1 tatataaata ggaagaaaac aaattttacg taattttttt cgaaaagcaa ttgatataat
       61 tcttatttca ttatacaatt tagactaatc tagaaattga aatggagtaa tattttgaaa
      121 aaagaattga ttatttgtcg aataagcaga ataagtattc gattagacgt tttacagtag
      181 gtaccacatc agtaatagta ggggcaacta tactatttgg gataggcaat catcaagcac
      241 aagcttcaga acaatcgaac gatacaacgc aatcttcgaa aaataatgca agtgcagatt
      301 ccgaaaaaaa caatacgata gaaacacctc aattaaatac aacggctaat gatacatctg
      361 atattagtgc aaacacaaac agtgcgaatg tagatagcac agcaaaacca atgtctacac
      421 aaacgagcaa taccactaca acagagccag cttcaacaaa tgaaacacct caaccgacgg
      481 caattaaaga tcaagcaact gctgcaaaaa tgcaagatca aactgttcct caagaagcaa
      541 attctcaagt agataataaa acaacgaatg atgctaatag catagcgaca aacagcgagc
      601 ttaaaaatcc tcagacatta gatttaccac aatcatcacc acaaacgatt tccaatgcgc
      661 aaggaactag tgaaccaagt gttagaacga gagctgtacg tagtcttgca gttgctgaac
      721 ctgtagtaaa tgctgctgat gctaaaggta caaatgtaaa tgataaagtt acggcaagtg
      781 atttcaagtt agaaaagact acatttgacc ctaaccaaag tggtaacaca tttatggcgg
      841 caaattttaa agtggcaggg aaagtgaaat caggggatta ttttacagcg aagttaccag
      901 atagtgtaac tggtaatgga gacgtggatt actctaattc aaataatacg atgccaattg
      961 cagatatagt gaatgataaa aaagaagttg tagctaaagc aacatatgat atcttgacta
     1021 agacgtatac atttgtcttt acagattatg taaatgataa agaaaatatt aacggacaat
     1081 tttcattacc tttatttaca gaccgagcaa aggcacctaa atcaggaaca tatgatgcaa
     1141 atattaatat tgcggatgaa atgtttaaca atcaaattac ttataactat agttcaccaa
     1201 ttgcaggaat tgataagcca aatggcgcga acatttcttc tcaaattatt ggtgtagata
     1261 cagcttcagg tcaaaacaca tacaagcaaa cggtatttgt taaccctaag caacgagttt
     1321 taggtaatac gtgggtgtat attaaaggtt accaagataa aatcgaagaa agtagcggta
     1381 aagtaagtgc tacagataca aaactgagaa tttttgaagt gaatgataca tctaaattat
     1441 cagatagcta ctatgcagac ccaaatgact ctaaccttaa agaagtaact aatgagttta
     1501 aggataaaat cacttataaa taccaaaacg tagcaagtat taattttggt gatataaata
     1561 aaacgtatgt cgttttagtc gaaggtcatt atgataatac tggtaaaaac ttgaaaacac
     1621 aggttattca agaaaatatt gacccagcga caggtaaaga ttacagtatt ttcggttgga
     1681 ataatgagaa tgttgtacgt tatggaggcg gaagtgctga tggtgattca gcagtaaatc
     1741 cagttgatcc aacaccggga ccaccagttg acccagagcc agaaccggag ccaacgccgg
     1801 atccagagcc aagtccagag ccagagccag aaccggaacc aacgccagat ccagaaccaa
     1861 gtccagaccc ggatccggaa ccaacgccag atccagagcc aagcccagaa ccagatccgg
     1921 attcggattc ggacagtgac tcaggctcag acagcgactc aggttcagat agcgattcgg
     1981 attcagatag cgactcggat tcagatagcg attcggattc aga
//
LOCUS       DAKSQE010000204         1978 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.207, whole genome shotgun sequence.
ACCESSION   DAKSQE010000204 DAKSQE010000000
VERSION     DAKSQE010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.207"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            76..441
                     /locus_tag="O0V33_002667"
     CDS             76..441
                     /locus_tag="O0V33_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HCX8977243.1"
                     /translation="MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKNLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
     gene            623..1900
                     /locus_tag="O0V33_002668"
     CDS             623..1900
                     /locus_tag="O0V33_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCX8977244.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKDITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
BASE COUNT          696 a          263 c          346 g          673 t
ORIGIN      
        1 acaatctggt aaaaactctt tattaaataa attgattcgt tcatactgaa tagataagca
       61 aaaaggagtt acataatgaa gaagaaaaaa aacgcgatta aagttatcat tatattattt
      121 atttcattaa ttgttgctgt agcatttttc tttggtctga aaacatatca aggccataag
      181 aatattcaac ttattgacag ttacttggaa gagaaaaatc taaaagataa aattaaatca
      241 gaaaaaacag agtatagtgc taaaaaaggt ctcttctata aggaagttac gtttaaagat
      301 gaacctggtg taacttatgt tgtgcaacca atcagtacta ataaaggatt atttgttgaa
      361 ggctttgata cagagactaa gaaaagtttg aaaactgcaa agcataaata tttcaatcag
      421 aattataaac catcaaaata gtgaaacgta taactggtga aacagtttag tgcctgcaat
      481 tttaattatt taaggtaaac agtttttgta tggaaattag tgatttttga catctataaa
      541 agataaatat tttaattttt tatagataat tatcaatttt ctgatataat tgagtgatgt
      601 cagatgaagg ggagtttgta ctatggctct attcaagaga aaaattagcc tacctatgca
      661 agtagttatt gcattagtgt taggtgttgt cgtaggactt ttattatatg gacaagaaaa
      721 cgtagcaaat tacattaaac catttggtga cgtattttta aatttaatta aaatgatcgt
      781 tataccagtt gtattttgct cactagcgct ttctatttcg aacgttgggg aatcgaaaac
      841 tgtagggcgt tatggctgga aaacaatttt atactttgaa attattacaa caatcgcaat
      901 aggtttaggg attatcttcg gtaacctatt taaaccaggt gctggattag acccaacaaa
      961 attacctaaa ggtgatattt ctaaatatca atcaactgca catgcagcag aacaatctac
     1021 atatggaaat cattttattg ataccattgt acatattatt ccgacaaact tttttgaagc
     1081 tttaaataag ggtgaattat tacctattat ctttttcgca gtattctttg gattaggatt
     1141 agctgcagta ggtaaaaaag cagaaccagt taaagaattt ttaagcggat cgcttgaagc
     1201 tgtgttctgg atgattaata aaattttaaa attagcacca cttggagtgt ttgcattcat
     1261 ttgtactaca attattacat ttggtgcatc agcattatta ccactattaa aattagtatt
     1321 agttgttgtc tttgcaatgg tgttctttgt attcgctata ctaggactag ttgcatggat
     1381 gtgtggtatt aatatcatga atattattag aatcttgaaa agtgaattgc ttttagcatt
     1441 ttctacatca agttcggaag ctgttcttcc tgtaatgatg aagaaaatgg aaaactttgg
     1501 ttcaccaaaa gatattacat cctttgttat accgattggc tatacgttta acttagacgg
     1561 atcagcactt tatcaatcta ttgcagcatt attcgttgca cagatgtatg gaatgcactt
     1621 aacattatca gagcaaattg tgttgatgtt aacattaatg attacatcta aaggtatggc
     1681 ggcagtacca ggaacttcaa tcgttgtttt attaacaaca ttaggcgcca tgggcttacc
     1741 ggcacaaggt ttagcattaa ttattggtgt tgaccgtatc ttagatatgg tacgtacatg
     1801 tgtaaacgtt attggtaacg cattatcaac aattgttatt gctaaatggg aaaacgtata
     1861 tgataaagca aaaggtcaag aatatttaaa atcaatttaa aaaatactat ctgacattta
     1921 aagagtctgg gacataaatc cctaaaaaaa cagcagtaag atattttcta attgaaaa
//
LOCUS       DAKSQE010000205         1971 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.208, whole genome shotgun sequence.
ACCESSION   DAKSQE010000205 DAKSQE010000000
VERSION     DAKSQE010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.208"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(224..1510)
                     /locus_tag="O0V33_002669"
     CDS             complement(224..1510)
                     /locus_tag="O0V33_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCX8977245.1"
                     /translation="MNPLAQSLNEQLQQSNATAFAMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSRPIVTNALTHGLSLVGDLFVNQDDTILLPEHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVEAIKA
                     LANKGTKVIAVVDDAYYGLFYEDVYTQSLFTALTNLHSNAILPIRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEALRKHLIDKYSIG
                     VIALNATDIRIAFSCVEKDDIPHVFDSIAKAIDDLR"
     gene            1710..1808
                     /locus_tag="O0V33_002670"
     CDS             1710..1808
                     /locus_tag="O0V33_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977246.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
BASE COUNT          629 a          303 c          349 g          690 t
ORIGIN      
        1 catttgtaga gatgtgatac ttgaagtgat ttgccacacg tttgataaat ttatctaagg
       61 tgtatcaagt tttgttagga gagatgtata aaactaatta ggtatagcga ttaataagtt
      121 gctgaataaa atatatctta tgatgctttg aaagaaggaa taattttaaa aataaaaaac
      181 caataatccg agtcatacac atcggattat tggttgaagt taattatctt aagtcatcaa
      241 ttgctttagc aattgaatca aatacatgtg ggatatcgtc tttttctaca caactaaacg
      301 caatacgaat atctgtagca ttaagtgcaa tgacaccaat agaatacttg tcaattaaat
      361 gcttgcgcaa tgcttcaggg tcaacatcat gaacttttat tgccataaag tatccagagt
      421 taaagtcata tgcttgccaa tgtgaatgat attgatcagc atagacaact tccttagtga
      481 cttcatagcg ttcttttaat gtttgaatat tttgttcgat ttctttatcg aattgtttat
      541 tattttttaa tacatgctta acagcgcttt gtgttggaag cggtccacta gaaatgttgc
      601 ttcgtataag accttttact ttggcttcta aaacttcttt agttgtttga tcagacgttc
      661 caaatgtcat aaatccaaca cggaatcccc atgcgaaaaa ttcttttgtt gccccatcta
      721 aacgaatagg taatattgca tttgaatgta aattagttaa tgcagtaaat aatgattgag
      781 tatacacatc ttcatagaat aaaccatagt atgcatcatc cacaacagct ataacttttg
      841 tacctttatt agctaatgct ttaattgctt cgacgatcgt agtcacttct ttatgcgtag
      901 gtgtgtaacc tgtcggatta ttaggataat ttaaaatcat aatgacttta tctttattgt
      961 atgattgtaa agcttctact agtgaatcag tagtataatg cccgtcttta tcaaagatag
     1021 gatatgtttg aaggtttgca ccatttcttg tattgaaaac aagtttgtaa ttaccccaat
     1081 tatgctctgg taacaagata gtgtcatctt gatttacaaa taaatcgcca actagagata
     1141 aaccatgtgt taatgcattc gtaacaattg gtcgtgacat gttgtcgatt gatagctctg
     1201 gattgtcacg caacattttt tgttgccata aatcacgtaa ttcttcgatg ccttgtggtg
     1261 gcgcataagg gaatatttca tctggagtta aatcattaaa cattgcatct aaagatgatg
     1321 caaacatttt tccgtcttta tttgtcgcca tacctatagt tgcattatat gttgtactct
     1381 ttgcttcagc agattgagat aaaatgccct ttggataaaa catattttga cctaagtcag
     1441 ataacattgc aaaggcagtt gcatttgact gttgaagttg ttcatttaag ctttgggcta
     1501 aaggattcat aattgcttaa cctctcttta aaaaaattgt atgcctatat attatcttca
     1561 attataccaa agataaagtg ttgttggtga attaacgaat aatatgtaaa atccgctacc
     1621 aaaatacgaa cacatgttct gtttttgtgt tagaataggt gtaggtcatc cttcagtatg
     1681 atactttagt cgaacggagg tgtagtgtaa tgatgtggtg gcaagaaggg atgatgacac
     1741 ttattacagg tggcttacta attatttttc ggctttggtt agaactgaaa tggaaaaata
     1801 aaaaatgatc ctacacacat aaataacccc aagtctgctg gtaccagact tggggttgtg
     1861 tagtataatt ctgtggatac acttaaataa tatcattgaa cacaatatat cgcaatcaat
     1921 aatcattttg aatgcatagt tttgttgtca tgtaagccaa tgagagattg g
//
LOCUS       DAKSQE010000206         1949 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.209, whole genome shotgun sequence.
ACCESSION   DAKSQE010000206 DAKSQE010000000
VERSION     DAKSQE010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.209"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..591)
                     /locus_tag="O0V33_002671"
     CDS             complement(<1..591)
                     /locus_tag="O0V33_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCX8977247.1"
                     /translation="MSQNTNHSYYHQNQHAQAISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFYTI
                     ITVVSLLLFLLYTLYDFNRLKRGDYSPREMGFNLFIN"
     gene            952..1749
                     /locus_tag="O0V33_002672"
     CDS             952..1749
                     /locus_tag="O0V33_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCX8977248.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNII
                     ANRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
BASE COUNT          726 a          328 c          278 g          617 t
ORIGIN      
        1 attaataaat agattaaatc ccatttctct tggtgaatag tcaccccttt ttaaacgatt
       61 aaaatcatac aaagtataaa gtagaaataa caacagacta acgacggtaa taatagtata
      121 aaaaatagga ttttgaagaa aaataccaat tagactcgca ataattaatg ttattaatgt
      181 aacgaataaa tacttgccta tactcgatgc atctccaacg aagaaataac caataatacc
      241 aaatgctata aatgcaaaaa ttgctaatgc gatattttta tagaaaatat ctggtcctaa
      301 attttgtaaa tacgtggtaa acgttgcgta tgacaataag ccaatcacta cagcgtatat
      361 atgtgaaata attaaaccga accgtctcgc tctttcaaaa acaagtgttg ctaaaataat
      421 aatcagtaat ccaaatgaca agggttgtcg ccaacttaat ggtaaaaact gacctagata
      481 gcatcctatg ccaaatataa tccaataata cataaaataa agccacactt tacttattgc
      541 ttgagcatgc tggttttgat gataatatga atgatttgta ttttgcgaca aaataattcc
      601 tccttctact gtcgttttat gcgtcaaatt ttacagtaaa ttttgcttca tcaaaagaaa
      661 taacctttaa caagtatagt aattttacat tttataatgt tacaaaatat aattttttat
      721 aattagttaa aatcactaaa acgcttttat acactatcaa atcagcattt ataaaaatat
      781 gaaccgatat cctaaaatgt taataatatt acaagataat aacaaaccac acaaagctac
      841 ttatttttga taatatggaa agcgtaatat aaaacgaaaa cttaatttac tatataaatt
      901 gtcttaataa tttttaaaag tagtaaatct taattttaag gaggagtccc tttgaaaaaa
      961 ttagcatttg caataacagc aacatctggt gcagctgcat ttttaacgca tcatgatgcg
     1021 caagcttcta cacaacatac agtacaatct ggtgaatcat tatggagtat tgctcaaaaa
     1081 tacaacactt cagtagagag tattaaacaa aataaccaat tagataacaa cttggtattc
     1141 cctggtcaag ttatctcagt aggtggaagt gatgcacaaa atacgtcaaa cacttctcca
     1201 caagctggtt cagcatcatc tcatactgta caagctggtg aatcattaaa tatcatcgct
     1261 aacagatatg gtgtttcagt tgatcaatta atggcagcca ataacttacg tggttattta
     1321 attatgccta accaaacatt acaaattcct aatggtggat caggtggtac aacaccaaca
     1381 gctacaacag gtagcaatgg caatgcatca tcttttaatc accaaaattt atacactgct
     1441 ggtcaatgta catggtacgt atttgaccgt cgtgctcaag ctggtagtcc aattagcaca
     1501 tattggtcag acgctaagta ttgggctggt aacgcagcta atgatggtta ccaagtaaac
     1561 aacacaccat cagttggttc aatcatgcaa agcacacctg gtccatatgg tcatgttgct
     1621 tatgttgaac gtgtcaatgg tgatggtagt atcttgattt ctgaaatgaa ttacacatat
     1681 ggtccataca atatgaacta ccgtacaatt ccagcttcag aagtttctag ctatgcattc
     1741 atccattaat taaataaatt gtactgatat atactagcaa ttcacatcat gtgagattgc
     1801 tagtttttta tttttgaaaa aaattttcat tttggtacaa aaaattatct cacccttccc
     1861 tatcatactt atttatattt tgtatgaatg gtagttaggt aaaaaataat aacctaccta
     1921 tttgattaaa ttaaatgcat aatatatta
//
LOCUS       DAKSQE010000207         1927 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.210, whole genome shotgun sequence.
ACCESSION   DAKSQE010000207 DAKSQE010000000
VERSION     DAKSQE010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.210"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            75..1685
                     /locus_tag="O0V33_002673"
     CDS             75..1685
                     /locus_tag="O0V33_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HCX8977249.1"
                     /translation="MNLSDALSRSVSDMSYENLDKDQTYDFLGQDYKVIEKRDDTQNG
                     FRAYVFAPVIKGKVDKSQLLIGYAGTDPYSLNDIRTDLQLPIHHNTNNLKINNHYKDL
                     KNSTIHKKYNTIKDKNDLFSFSSNLNNKALFGDLWAAKFAPTQMDESDAFTESVKKKY
                     PDSEIHPLGHSLGAFLAQYNLIKHNLDNGTTFAAPNVYHSFTGDFKKDIDNGVYDSKI
                     RNIGHFDDPINNLNFFNPRIGKNITSMPHYDGFITNLPLIGQHTLKSYNDFDADGNVK
                     EMPELEQFNDDQSLNNRFQLFDPFGIKSRFFDSIKIDSSILNRTSENDFDPFAILNKK
                     DKLFESNDLDIEGFNNKQKEDLSIDLNPFDDELNDEDEPLIPKRGDSKKLKAEKKISV
                     KKTKSSSKNSGKKVIIQPDEVRNIASTIRDRLFLYTQIITALDEYREETMSGANRILD
                     KYQSELLSGSHEFITPNDLEQYMEILSVEGSSGNFKFYNSGLMDDLIQALDQNKKDLL
                     QFAEKLEFAANKFEEKDLEESDVFGLFS"
     gene            1700..>1927
                     /locus_tag="O0V33_002674"
     CDS             1700..>1927
                     /locus_tag="O0V33_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HCX8977250.1"
                     /translation="MSSKDAKKSQEEARLQQLKQEMRNSTDNMVSNAKNNVALKFQTL
                     NEIIETINNSSQSLEGTFEGKASEAAQQSINK"
BASE COUNT          763 a          271 c          298 g          595 t
ORIGIN      
        1 attaaaataa taactttagt tttccgaatc atattttacc tcacttttaa atttttaata
       61 aaggagtttt tattttgaat ctttcagacg ctctttcaag aagtgtttcc gatatgtctt
      121 atgaaaattt agataaagat caaacatacg actttcttgg ccaagattac aaagtcatcg
      181 aaaaacgcga cgatacccag aatgggttta gagcatatgt tttcgctcca gtaatcaaag
      241 gtaaagttga taagagtcaa cttttaatag gttacgctgg cactgaccca tatagtctaa
      301 atgatattcg tactgattta caacttccca ttcatcataa cacaaataat ctaaaaatta
      361 ataatcatta taaagattta aaaaactcaa ctattcacaa aaaatacaat acaataaaag
      421 ataaaaatga cctttttagt ttttcttcta atttaaataa taaagcactt ttcggtgatc
      481 tatgggcagc taagtttgca ccgactcaaa tggacgaaag tgatgctttt actgaatcag
      541 ttaaaaagaa atatccagat agtgaaattc acccattagg acatagttta ggtgcgtttt
      601 tagctcaata taatttaata aaacataatc tggataacgg tacaacgttt gcagcaccta
      661 acgtatacca ttcatttact ggagacttta aaaaagacat tgataatggt gtctatgatt
      721 caaaaatacg caatattggc cattttgatg atcctattaa taatttgaat ttttttaatc
      781 ctcgtattgg caaaaatatt acatcaatgc cacattatga tggatttata actaaccttc
      841 ctttgattgg tcaacacacg cttaaatctt acaatgattt cgatgcagat ggtaatgtga
      901 aggaaatgcc agaacttgaa caatttaatg atgaccaatc tcttaataat agatttcaat
      961 tatttgaccc atttggtata aaaagcagat ttttcgactc tattaaaatc gatagttcta
     1021 tattaaatag aacaagtgaa aatgattttg atccttttgc tattctcaat aaaaaagata
     1081 aattattcga atcaaatgac cttgatatag aaggatttaa taataaacaa aaagaagatt
     1141 taagcattga tttaaatcct tttgatgatg aattaaatga tgaggacgaa ccattaattc
     1201 ccaaaagagg cgatagtaaa aaattaaaag cagaaaagaa aatctctgtt aaaaaaacca
     1261 aaagtagttc caaaaattca ggcaaaaaag taataattca gcctgatgaa gtgcgtaata
     1321 tcgctagcac aattcgtgat agactatttt tgtatacaca aataattact gcattggatg
     1381 aatatagaga agaaacaatg tcaggggcta atcgcatttt agataaatat caatcagagc
     1441 ttctaagcgg ttcacatgaa tttataacac ctaacgatct tgaacagtat atggagattt
     1501 tatcagttga gggaagttca ggtaatttca agttttataa tagtggttta atggatgact
     1561 taattcaggc attagatcaa aacaaaaaag acttgctaca atttgctgaa aaactagaat
     1621 ttgctgcaaa taaattcgaa gaaaaagatt tagaagaaag cgatgttttt ggactattta
     1681 gttaaagagg gtaatttata tgagctcaaa agatgcaaag aaaagtcaag aagaagctag
     1741 attacaacaa ttaaagcaag aaatgcgtaa tagtactgat aatatggtta gcaatgctaa
     1801 aaataatgta gcattaaaat ttcaaacatt aaatgaaatc attgaaacaa ttaataattc
     1861 ttcgcaaagt ttagaaggta catttgaagg aaaagcaagc gaagcagccc aacaaagtat
     1921 aaataaa
//
LOCUS       DAKSQE010000208         1919 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.211, whole genome shotgun sequence.
ACCESSION   DAKSQE010000208 DAKSQE010000000
VERSION     DAKSQE010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.211"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            128..856
                     /locus_tag="O0V33_002675"
     CDS             128..856
                     /locus_tag="O0V33_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HCX8977251.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESTSHKKKENDNEELNE
                     ELKEFKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDK
                     DLNPSSISHDYINITQDDKNTVNKLRDGYLLSDKNYKDWTEHNQDQIKKGKTAQAMFI
                     YELRGDGNINLNVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFEEEY
                     KKEQEREKEKEKQKDDDHSGLDEV"
     gene            complement(1144..1758)
                     /locus_tag="O0V33_002676"
     CDS             complement(1144..1758)
                     /locus_tag="O0V33_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HCX8977252.1"
                     /translation="MNDLSLSSFLKRSNKFMQFNCFICLILIIVFYIIGMNIQDFSDF
                     PSKDLNHKVTYNLNGFLEIFVNNAFIVPFVSLILSIIPIPYLYFIPTISTIYSLSVII
                     GVTFSYKLNEGIAIFIGILPHGILEIYLTSIELSMLFLLNAYIRKNSMNLFRKRKETL
                     PNFFVLLKSIVKCYLLIFLPVAFLCALIEITVTPTVYKFLTNII"
BASE COUNT          823 a          254 c          249 g          593 t
ORIGIN      
        1 ttattacaag aattatttaa tatcatctta gaatctcaca aaatatcaac tttgtttaat
       61 tactcaaaac gataagtcgt ttagagtttt taaaataaat ttttgcgaaa taaaggagac
      121 atgttaaatg aaaaaagtaa tggggatatt attagcaagt acacttatct taggtgcttg
      181 tggacatcat caagatagtg caaaaaaaga gagcactagt cacaaaaaga aagaaaatga
      241 caatgaagaa ttaaatgaag aacttaaaga atttaaaagc aaaaaaaata tggatataaa
      301 aattaaaggc gatactattg ttagtgacaa atttgaagct aaaataaaag aaccgtttat
      361 catcaatgaa aaagatgaga aaaagaaata tatcgctttt aaaatggaaa ttactgctaa
      421 aaaagacgat aaagatttaa atccatcttc tatttctcat gactatatta atatcactca
      481 agatgataaa aatacagtaa ataaattaag agatggttat cttttaagtg ataaaaatta
      541 taaagattgg acagaacata accaagatca aattaaaaaa ggcaaaactg cacaagccat
      601 gtttatctat gagttaagag gtgatggaaa cattaattta aatgtccata aatactcaga
      661 agataaaaca gttgattcta aatcattcaa atttagtaaa cttaaaaccg aagatttttc
      721 tcatagagcg gaaacaaggg aagaagtaga aaagaaagaa aaagaatttg aagaagagta
      781 caaaaaagaa caagaacgag agaaagaaaa agaaaagcaa aaagatgacg accacagtgg
      841 tttagatgaa gtataaattt atgaatgctc gcattcagcg ttataatctt tgttgttatt
      901 attgtcatta taaattagct tacttacact cataatgaca cactaattta attgttaaat
      961 ataacaaaaa agagtagctc gcctactctt ttttgttgtt ttgaaatggt catttttgaa
     1021 atgactcgat tatagtttca cgaataccat gcaacatttt tcgtgaactc atattttaca
     1081 aatcaatatt tatcgatcaa aaataaacct atacacatag ttagctatgc ccttttaaaa
     1141 gttctatatt atattagtta aaaatttgta aacagttggt gtaacagtaa tttcaattaa
     1201 agcgcataaa aatgccacag gtaaaaatat tagcaggtaa cactttacta ttgattttaa
     1261 taaaacaaaa aaatttggca atgtctcttt tctttttcta aataaattca ttgaattttt
     1321 tctgatgtac gcatttaata agaataacat tgatagttca atgcttgtta agtatatttc
     1381 taaaatacca tgaggcaaaa taccaataaa aatagctatc ccttcgttta atttatatga
     1441 aaatgtaacc ccaataataa ccgacaatga ataaattgta gatattgtag gaataaaata
     1501 caaatatggt attggtatta tcgataatat aagtgacaca aaaggaacta taaaagcatt
     1561 attaacaaat atctccaaaa acccattaag attataagtc actttatgat ttaaatcttt
     1621 actaggaaaa tcactaaagt cctgtatatt cataccaatt atgtaaaata ctattattag
     1681 aatcaaacaa ataaaacaat tgaattgcat aaatttgtta ctgcgtttca aaaatgaaga
     1741 taaactcaag tcattcattg attatgcccc catctataaa tactatttca ctaaatagat
     1801 acgttcactc gcaaacgata tcatccttaa aaatctatta aaaaccagct atattaaaac
     1861 ctttaattta ccagctaaaa ctatcacaac ttttcctcat tacatttaaa caatatatc
//
LOCUS       DAKSQE010000209         1908 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.212, whole genome shotgun sequence.
ACCESSION   DAKSQE010000209 DAKSQE010000000
VERSION     DAKSQE010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.212"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            188..893
                     /locus_tag="O0V33_002677"
                     /pseudo
     CDS             188..893
                     /locus_tag="O0V33_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            1285..1821
                     /locus_tag="O0V33_002678"
     CDS             1285..1821
                     /locus_tag="O0V33_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977253.1"
                     /translation="MHNKNEFNKKLKDFIGSDKRMALVKGYINEYKLTTVLRALNNSD
                     YTKGTIHTRSIGNLKQIVDNRIIPKNITQNTIFKLSNLTLEVNLYERNTIYHGDFAIY
                     YPVESALIIETDTPRLINHINNNPVQKIFIITTNDWSFNTLELEKIVDETIIYDLKQE
                     DPKQYEILYKNKHGKLPY"
BASE COUNT          799 a          288 c          274 g          547 t
ORIGIN      
        1 tacatgaact ataaaaagcg gataaatagg aggtcattgc gaaatttgtt aaatacaaaa
       61 ttgatgagtt ccacctaata aaattgttgg ttttatagta acatctattt tcgtaccatt
      121 catgttaatt tcagtgctaa taatgctgat ataaagaaga ataaattgat taggagaaca
      181 ctatttaatg tctaaaaata tcaatcaagc aaatagtaaa ttaaatacgt caaacaaaaa
      241 actaaaacaa gcttattcaa aatccgatag taaaaacttg aaagttatat acatgcctca
      301 ttggctagag ttttattcaa ttgctataca tagcgatgtt acaagcaata aaaaaagata
      361 ttataaatca tatagtagag gaacagtggt atatgtaaaa ctagggagta atattggaag
      421 tgagttttct ggtaatcatt tttgtgtaat attagataac aaagacaata aaggaaaaga
      481 aacagttaca atagttccac tttcttccaa aggtaataaa aattatttga agttaaacga
      541 atcggtgcta aatctaacaa caacagattt aaaaaaaaca gattatcgat atcagtagta
      601 aagtacaagc attagcccaa aaatatagag atattgacat taaacttagc acagaagata
      661 aaaagctttt gtctaactta aatcaaaaag ctaatgaact gtatagagtg ataggtgttt
      721 attccaagca caaagggaaa gatacatacg ttaatatatc agcaattaca acaataagca
      781 aaagacgtat aagcaaaata aatgattctg atcctactgg aacgataata atttcagaag
      841 atgacatgaa tgaaatcgaa aagcaattaa agataaagtt cttttctaat tgaattgtaa
      901 tattacatct gatataattg gtttacaaat tcccggtaac cattccggct tcattctctt
      961 gaagaatgac atattctcag cgttttagct gaaggtcaga tgatacgtca tctggcctct
     1021 ttttttatat aaatgccctt taagtgaaat atacaacaat ctgcaaccaa tatagacatc
     1081 acaaaacaca caataaatta cacaatgtgt tataagccac atgtcactat tatcgtagct
     1141 agctgcgtca ctcaaaacga atacggaacg cgttggttat tccaatcata atattactct
     1201 gcaaatacac agccctgtaa ccgagtaaat ggatagagat ttgaacaaat gaaaacaatc
     1261 aactaatgga aaggataaaa tattatgcac aacaaaaatg aattcaacaa aaaactgaaa
     1321 gattttatag gtagcgataa acggatggct ttagtaaagg gttatattaa cgagtataaa
     1381 ttaacaactg ttttaagagc tttaaataat tccgattaca caaaagggac gatacacact
     1441 cgttcaatag gcaacctaaa acaaatagta gataatcgaa ttattcctaa aaatataact
     1501 caaaacacaa tcttcaaatt gagtaattta accttagagg ttaacctcta tgaaagaaac
     1561 acaatttatc acggagactt tgctatttac tatcctgtag aatctgctct aataatagaa
     1621 acagatactc ctaggctaat taatcatata aataacaacc cagtccaaaa aatatttata
     1681 ataacaacaa atgactggag ctttaataca cttgaattag aaaaaatagt agacgaaact
     1741 ataatttatg atttaaaaca agaagatcct aaacaatatg aaattttata taaaaataaa
     1801 cacggaaaat tgccttattg atgaattaaa acgcaaactt agaacacctt accccttttc
     1861 cgtccttatt gtaattttaa atcatcaaga ggctgggaca taaatccc
//
LOCUS       DAKSQE010000210         1879 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.213, whole genome shotgun sequence.
ACCESSION   DAKSQE010000210 DAKSQE010000000
VERSION     DAKSQE010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.213"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            235..357
                     /locus_tag="O0V33_002679"
     CDS             235..357
                     /locus_tag="O0V33_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977254.1"
                     /translation="MSYIIKFTFKFFEMTTYISQLTLDIELVSSELLVILSGFI"
     gene            complement(361..1155)
                     /locus_tag="O0V33_002680"
     CDS             complement(361..1155)
                     /locus_tag="O0V33_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCX8977255.1"
                     /translation="MIQSKKLTLGICLVLLIILIVGYVIMTKTNGRNAQIKDTFNQTL
                     KLYPTKNLDGFYDKEGFRDQEFKKGDKGTWIVNSEMVIEPKGKDMETRGMVLYINRNT
                     RTTKGYYFISEMTDDSNGRPKDDEKRYPVKMEHNKIIPTKPLPNDKLKKEIENLKFFV
                     QYGNFKDINDYKDGDISYNPNVPSYSAKYQLNNDDYNVQQLRKRYDIPTKQAPKLLLK
                     GDGDLKGSSVGSKNIEFTFVENKEENIYFTDSVQYTPSEDTRYESN"
     gene            complement(1265..1758)
                     /locus_tag="O0V33_002681"
                     /pseudo
     CDS             complement(1265..1758)
                     /locus_tag="O0V33_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
BASE COUNT          523 a          325 c          229 g          802 t
ORIGIN      
        1 aaatgtagat gttctttaga cattaaaagc ttctaacatg atacggaata tgaggttcct
       61 gtatcacatt agaagctttt ttgttgtggt gcttatattg ataatggcat gaatgaacac
      121 acgctgtctt agtttcagga atcggctaga atattaaact tccgttcttt gaatattttt
      181 taatttctta ctaatatcat ccagttcatt cttcgccttt ttaacccaat ctccttgagc
      241 tacattatca aattcacctt taaatttttt gaaatgacta cctatatttc acagctcact
      301 ttagatatcg aacttgtttc ttcagagtta cttgttatat tatcaggttt tatttgatat
      361 tcagtttgac tcataccttg tatcctcact tggagtatat tgtacagaat ccgtaaaata
      421 gatgttttct tctttatttt ctacaaaggt aaattctata ttttttgatc ctactgatga
      481 tccttttaaa tcaccatcac ctttcaataa tagtttcggc gcttgtttgg ttggaatatc
      541 atatcttttt cttaactgtt ggacattata atcatcatta ttcaattgat attttgccga
      601 ataacttggt acattaggat tatatgaaat atcaccatct ttataatcat taatatcttt
      661 aaagttgcca tattgtacaa agaacttaag gttttcaatc tcttttttta acttgtcatt
      721 cggtagtggc ttcgttggta tgattttatt atgttccatt tttaccggat accttttttc
      781 atcatccttt ggtctgccgt tactgtcatc tgtcatttca cttataaaat aatacccttt
      841 tgtggttcta gtattgcgat tgatatagag caccattcct ctcgtttcca tatccttacc
      901 ttttggctcg attaccattt cagaattaac tatccaagta cctttatcac cctttttaaa
      961 ttcttgatct cgaaagcctt ctttatcgta aaaaccatcg agatttttgg ttggatataa
     1021 ttttaatgtt tgattaaatg tgtctttaat ttgggcgttt cgaccatttg tttttgtcat
     1081 aatgacataa cctacaatca atataatgag taataccaag catataccga gcgttaattt
     1141 ctttgattga atcatcattt ctccacctta tatttttaat actgactcaa tctttaattt
     1201 atacatctaa agagaaaaat tttttaacac ttaatggaat tttcattcca aatttctatt
     1261 aactctattc agtaggtttg aaattaatac tatcagaaaa atagatgttt tcttttttat
     1321 tttctacaaa agtaaattct aactctttag accctacaga tgagcctttt aaatcaccat
     1381 cgccttttaa taacattttc ggtgcttgtt tcgttggtat gtcatatctt tttcttaact
     1441 gttgcacatt ataatcatca ttcctcaatt gatatttttc ggaataactt ggcacattag
     1501 gattatatga aatatcacca tttttataat cattaatatc tttaaaattg ccatattgta
     1561 caaaaaattt aaagttttca atttcttttt tcaacttgtc atcttgtatt ggcttcgttg
     1621 ggataatttg attatgttcc attttgactg gatatttttt tctttttcat acgtttttcc
     1681 ttttgtttca cttacaaaaa aataacctgt tgtccttttt gtatttctat tcatatatag
     1741 aatcattcct cttgatttta aactttctcc ctttaattga atattcattt cagagctgat
     1801 tatccatgta cctttgtctc ctttgtcaaa ttcttgatct cgaaagcctt ctttatcgta
     1861 aaaatcatcg agatttttg
//
LOCUS       DAKSQE010000211         1873 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.214, whole genome shotgun sequence.
ACCESSION   DAKSQE010000211 DAKSQE010000000
VERSION     DAKSQE010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.214"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(187..1674)
                     /locus_tag="O0V33_002682"
     CDS             complement(187..1674)
                     /locus_tag="O0V33_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977256.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPLMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNYATSTLISLFIIYYCINAIIYKEKQPVSVTVLF
                     YVLSFFGQLFMENVTLFHCIILILAFLYEFTANRTLNYKLLFSFMIATIGTIIMFSNP
                     NYRKILFEGSEYQQVSNNQGIFSKFAEMISTSLPYGVIFSQVIILSMIAALIIYLLLR
                     SDRYLHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTDIGLVGFANIIVCGYFAFS
                     IFVGIYLSINDRKTQITLYSLLISICVSASTLVIVTPIEPGNFLIVYTIHVIILIILL
                     KEFRKYKSINVNFIKGASLVLAVIYLSAFIYVHYEHEKRVQLLKQQIKDHPRQEVYTV
                     ESLPFEHYMHHPSPINKNYQEWFNNYEELPKMTKVKYIPFGSDDS"
BASE COUNT          719 a          277 c          247 g          630 t
ORIGIN      
        1 ataaaacaag tgactatata gactaagcta aaaaataaaa agacataatc atgattgttc
       61 ttgatgagca ctcaataaag tcaggttaaa agtccaactt ttgagatgca cattatcatt
      121 tcatgattat gtctactttt taatattttt atgctgctat tataaatttt tataggtaag
      181 aataagttat gaatcgtcag aaccaaatgg aatatattta actttggtca ttttcggtaa
      241 ttcttcataa ttattaaacc actcttggta atttttattg attggtgacg gatgatgcat
      301 ataatgttca aacggcaatg attccactgt ataaacctct tgtcgtggat gatctttaat
      361 ttgttgtttt aataactgaa ctcttttttc atgttcataa tgaacataaa taaatgcact
      421 aagatatatc acagctaaaa ctagtgatgc acctttaata aaattaacat tgattgactt
      481 atatttccga aattctttta ataaaataat taaaatgatt acatgtatcg tataaacaat
      541 caaaaaatta cctggttcaa ttggagtaac aatgactagt gtcgacgccg aaacacatat
      601 tgaaataagt aaggaatata aagtaatttg tgttttacga tcattaatag acaaataaat
      661 acctacaaat atcgaaaacg caaagtaacc acatacaatt atgttcgcaa aaccaaccaa
      721 tccaatatct gtatttttat ttaataaaaa ctgattgtaa aatagtaaat aataaagcgg
      781 taaagtgata aatcctatca taatgatacg acgctttaat attgttaaat gtaaataccg
      841 atcacttcta agcagtagat aaataataag cgctgcaatc atacttaaaa ttataacctg
      901 actgaaaatg acaccatatg gaagtgatgt cgatatcatt tctgcaaatt ttgaaaagat
      961 accttgatta ttagaaactt gttgatattc tgatccttca aataaaattt tgcgataatt
     1021 tggattggaa aacataatga ttgtacctat agtagcaatc ataaatgaaa acaataattt
     1081 ataattaagt gttcgatttg cagtaaattc atacagaaaa gctaaaatta aaatgataca
     1141 gtggaataat gtcacattct ccataaataa ttgaccgaag aagcttaaaa catagaatag
     1201 tactgtaaca cttaccggtt gcttttcttt atatataatt gcattaatac aataataaat
     1261 gataaagagt gaaattagtg ttgatgtcgc ataattataa aatcctgcaa accagccata
     1321 tgtatctgca taaatagcac ttggtaaaat taacatcaaa gaaaatgcta acaaataata
     1381 gcttgtccac gctttacaac gtgtaatgtg cataatcatc caaatgatac ccatactaat
     1441 gagaccatat gaaagccaac gtaaccagct tacatgtaca gctataattt caaagatatt
     1501 tccgatatag cgaccattaa gtgatgcaaa tcctactttt aaaatatcag tattataatt
     1561 gctaaaccat tgtaaatcat cgtgcatgag tggtagtaag atacccataa aagtataaaa
     1621 caataatatc gcaattaata tcaaagttgt cttgtgtaat tgaattgttt tcactttgct
     1681 aatcctcaaa tctagttaaa ttttcctcaa cttgtaggtc gaaaaattaa ttcaatattt
     1741 ttaatgtatt tctaattttc acctatgcat gtttcctcaa tcaaattaga taaacaaggt
     1801 atttaatatt acttttcaac attttatcta aatcgtccct cgtctttttc tatgacgaat
     1861 gattacactt gtg
//
LOCUS       DAKSQE010000212         1833 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.215, whole genome shotgun sequence.
ACCESSION   DAKSQE010000212 DAKSQE010000000
VERSION     DAKSQE010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.215"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            134..1353
                     /locus_tag="O0V33_002683"
                     /pseudo
     CDS             134..1353
                     /locus_tag="O0V33_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
BASE COUNT          705 a          192 c          309 g          627 t
ORIGIN      
        1 tctttttcta ttgattgaga aaatagttat ttatatagtt tttaaagaaa aaaagtgata
       61 atatttagct aatctaatga aaatggattt attggactaa aaatacattt taaatgagta
      121 gaggaggctg caaatgggtt acagaacttt aaaaagtatt tttcatgaac acaatgaaag
      181 taaaatgaag gaagagtata ttaagcgatt taattcttta gcatctttca atactaatat
      241 taatatcata cctatggaaa atggaaaaaa agttaatgat ttggaatatc ctctattctt
      301 tatggtgact aaaaatctat caaaaaacaa gaattaatat caattaatag tagaaaaatt
      361 gatagagcac ttaattcttt accatatgca gctagagaac aatattttaa tgatttatta
      421 atcgatgaat tacaaagtac taatgaaatt gaaaatgtat ttagtactaa acaagagatt
      481 gcacatgcgt taaataacca agcatcagac tttcttaagt tcaggggact cgtggatcaa
      541 tataaagaga tagaacttaa taaaaaaatt aaagttgata atgtaagaga cattagagcg
      601 atttatgata aattagtttc aaatgaaatt aacgaacaag ataagttaga tggagagcta
      661 tttcgtaaaa attttgtcgg tgtgcatgat gggtcaacga ataaatatat acatgttggg
      721 ttacaacctg aaaccaaaat tgttgaatat ataggtgaaa tgctaacatt tttaaaatat
      781 tttgatgcgc ctcagccgtt caaaatcatg gctagtcatt atatgtttga atatatacat
      841 cctttttatg atggcaatgg tagagttgga agatttataa ttgccaagct cttgagtgat
      901 tattatgata attatactgc tttgacattt tcatatgtta taaataggaa taaatcgaaa
      961 tattacaagg cgtttatgac tgcttcaaat catttgaatt gtggtgattt gacggaattt
     1021 attgacacaa tgcttgagtt attgatagca ggacaagaaa gaatacttga tgagttaata
     1081 ccaaaaatgg atgcgactga aaaattaacg ctttatttaa ctagtcatta taaaaagata
     1141 gattatgaat ttttgtatct cctttcaatg gataaattgt ttggaaataa aagaaataga
     1201 ttaacattaa ttgatttaga aaacatattg ggagtaggaa gagtaaaaat taataataca
     1261 attaaaaaat ttggtaacta cttagttaaa attaaaagta gaccaacaat ttatgagata
     1321 agtgatgaat ttctaaattc gattataaaa taaatgtaat atgtgattca gctatactta
     1381 ttgtatttgt aagattgcgt aattgcttca ttaaaaaata tatttgttga gagtggtgca
     1441 tgacaagtgt actgctcttt ttgttgtgta tcagtattgt gatgttttaa ttcgaatatt
     1501 atatgaatca ttatgaaatt taataaaaat aattgaaata attatcattt tttctgatat
     1561 actgttaaac ggtttggaat ttttaagttt acactgtatt ggttgatata actcaactaa
     1621 taattgcgaa cagagtattt caaattgaaa agtattatga gtgtgataca taaatcaaaa
     1681 ttgtaggctc aagggagtgg gacagaaatg atattttcgc aaaatttatt tcgtcgtccc
     1741 accccggcaa ggttgactag aattgaaaaa agcttgttac aagtgcattt tcgttcagtc
     1801 aactactgcc aatataactt cgtagagcat aga
//
LOCUS       DAKSQE010000213         1822 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.216, whole genome shotgun sequence.
ACCESSION   DAKSQE010000213 DAKSQE010000000
VERSION     DAKSQE010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.216"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            61..1590
                     /gene="aur"
                     /locus_tag="O0V33_002684"
     CDS             61..1590
                     /gene="aur"
                     /locus_tag="O0V33_002684"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HCX8977257.1"
                     /translation="MRKFSRYAFTSMATVTLLSSLTPAALASDTNHKPATSDINFEIT
                     QKSDAVKALKELPKSENVKNHYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDEAADKAFNAVKIDKNKAKNLQDDVIKE
                     NKVEIDGDSNKYIYNIELITVTPEISHWKVKIDADTGAVVEKTNLVKEAAATGTGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAYNFNDQTGQATLITNEDENFVKDEQRAGV
                     DANYYAKQTYDYYKNTFGRESYDNHGSPIVSLTHVNHYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTNLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPSHMKDYVYTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
BASE COUNT          704 a          261 c          374 g          483 t
ORIGIN      
        1 gaaaaattaa attaatattt tatttataag ctgtttttaa aatttcagga ggaatgaaat
       61 gtgaggaaat tttcaagata tgcatttaca agtatggcaa cagtaacgtt gctgagctct
      121 ttgacacctg cagcactagc gagtgatacg aatcacaaac cagcaacttc agatattaat
      181 tttgaaatca cacaaaagag tgatgcagtt aaagcattaa aagagttacc taaatctgaa
      241 aatgtgaaaa atcattatca agattacgct gttacagatg taaaaacaga taagaaagga
      301 ttcacgcatt acacgttaca accgagtgtg gatggtgtgc atgcgcctga caaagaagtg
      361 aaagtgcatg cggacaaatc gggtaaagtc gttttaatca acggtgatac tgacgcaaag
      421 aaagtaaagc cgacaaataa agtgacatta agcaaggatg aagcggctga caaagcattt
      481 aacgcagtta agattgataa aaataaagct aaaaacctcc aagatgacgt tatcaaagaa
      541 aataaagtcg aaatcgatgg tgacagtaat aaatacattt acaatattga attaattaca
      601 gtaacaccag aaatttcaca ttggaaagtc aaaattgatg ccgacacagg agcagttgtt
      661 gaaaaaacga acttagttaa agaagcagca gcaactggca caggtaaagg tgtgcttgga
      721 gatacaaaag atatcaatat caatagtatt gatggtggat ttagtttaga ggatttgacg
      781 catcaaggta aattatcagc atacaatttt aacgatcaaa caggtcaagc aacattgatt
      841 actaatgaag atgaaaactt cgtcaaagat gagcaacgtg ctggtgtaga tgcgaattat
      901 tatgctaaac aaacatatga ttactacaaa aatacatttg gtcgtgagtc ttacgataac
      961 catggtagtc caattgtctc attaacacat gtaaatcatt atggtggaca agataacaga
     1021 aataacgctg catggattgg agacaaaatg atttatggtg atggcgatgg ccgcacgttt
     1081 acaaatttat caggtgcaaa tgacgtagta gcacatgagt taacacatgg cgtgacacaa
     1141 gaaacggcga atttagagta taaagatcaa tctggtgcgt taaatgaaag cttttcagat
     1201 gtttttggat actttgtaga tgatgaggat ttcttgatgg gtgaagatgt ttacacacca
     1261 ggaaaagagg gagatgcttt acgaagcatg tcaaacccag aacaatttgg tcaaccatct
     1321 catatgaaag actatgtata cactgaaaaa gataacggtg gtgtacatac gaattctggc
     1381 attccaaata aagcagctta taacgtaatt caagcaatag ggaaatctaa atcagaacaa
     1441 atttactacc gagcattaac ggaatactta acaagtaatt caaacttcaa agattgtaaa
     1501 gatgcattat accaagcggc taaagattta tatgacgagc aaacagctga acaggtgtat
     1561 gaagcatgga atgaagtagg cgtggagtaa aaatatataa acaagaagaa gtaatgttaa
     1621 acacttataa ataattaaat tttaaataca gcttatatat ggggtatacc tacattgata
     1681 attgactttg tgggtgtacc ctctttttgt aattagatga gaggagtgtg agggttgtct
     1741 gccgaaagac tactcggtag tctaaaaatc attactagta gtaaatatgt gataattaaa
     1801 tgctgactta gaatacaaaa tt
//
LOCUS       DAKSQE010000214         1799 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.217, whole genome shotgun sequence.
ACCESSION   DAKSQE010000214 DAKSQE010000000
VERSION     DAKSQE010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.217"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(194..1129)
                     /gene="trxB"
                     /locus_tag="O0V33_002685"
     CDS             complement(194..1129)
                     /gene="trxB"
                     /locus_tag="O0V33_002685"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCX8977258.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSAPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
     gene            complement(1196..>1799)
                     /locus_tag="O0V33_002686"
     CDS             complement(1196..>1799)
                     /locus_tag="O0V33_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977259.1"
                     /translation="QMYNALAYNYYYLGEEDESHYYWDKLKQISKVEIGHAPWVIENS
                     KEVFDQHILPLLQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTY
                     LVQGLTLNKLDFIHRGLLTLYHNELFVSENDLMVAWINQGELIIAEKVDLTDVEPYIG
                     AFIYLYFKNQPRNVTKKQITTWLGITQYKLNKMIEFLLSI"
BASE COUNT          573 a          355 c          233 g          638 t
ORIGIN      
        1 taaagttata agtttaatat aacatataaa aaaactagaa ctcattgaaa cgcacaaatg
       61 atacgtacta agttctagtt ttctatataa tttgctttaa tttaaaagta agacattatt
      121 ttaacaatgt gatgtcacta ttaaaataaa tatccaaata atttacaacg caacatctta
      181 attcgacttc gatttaagct tgatcgttta aatgttcaat atattccgct gcactttgtg
      241 ctgcaatact accatcgcca gtagcagtga caatttggcg taaacctttg tcgcgaacat
      301 ctcctgctgc aaaaatacct ggtgctgatg ttgtcatatc atcttttgtc acaatataac
      361 caacatcatt tgtaatacct aagtctttaa atggtgctgt taatggtttc ataccaatat
      421 agatgaatac accatcagcc tcgtgtgttt cttctgaacc atcttttgta gacgttaatg
      481 tcacagaacc cactttgccg tctttttcat taattgattt caaagtatgg ctccaaataa
      541 agtctatttt atcatttttg aatgctctat cttgtaaaat acgttgtgca cgtaactcat
      601 cacgacggtg aacgattgtt actttgtcag caaatttagt taagaatgtt ccctcttcta
      661 ctgctgagtc accaccaccg ataacgaata ggcgtttatt tttaaagaat gcaccatcac
      721 atactgcaca ataacttaca ccgcgtccac caagttcttg ttcacccgga acaccaattt
      781 tcttgtattc tgcacctgta gcaataatga ccgctttagc tgttaattct ttgttaccaa
      841 agttaatcac tttatattcg cctttatctt ctacagattt aatatctcca tattgataaa
      901 ctgcaccaaa ctttttagcg tgttcaaaca tttttgtaga taaatctgga cctgtaatca
      961 tttcgaaacc agggaagttc tctacttctt ctgtattagc catttgaccg cctggaatac
     1021 ctctttcaat cataactgtt tttaaattag cacgtgatgc gtatactgca gcagtcatac
     1081 cagctggacc tgcaccgata attgctatat caaaatctat ttcagtcatt ttattaacgc
     1141 ctcctcatta ttaatcatta tgcgcattat ataataaatc taacttttca taaatctata
     1201 tgctcaagag aaattcaatc attttgttca gtttatattg tgttatgcct aaccatgttg
     1261 taatttgctt ctttgtaacg tttcgaggtt gatttttaaa atacaaataa ataaacgcac
     1321 cgatatatgg ctcaacatca gttaaatcta ctttttcagc aattatgagt tcaccttgat
     1381 taatccatgc aaccattaaa tcattttcac ttacaaataa ttcattatgg taaagcgtta
     1441 acaagccgcg atgaatgaag tctaatttat tgagcgttaa accttgaact aaatacgtta
     1501 aatacaattt ctcataatta tttagatttt ccagaacctg ccaaatactt tccgtcatca
     1561 caatttcttt accatttaat tgatccaata aaaaaatacc atataaacga taatgactgt
     1621 catcactttg aagtaatggc aaaatatgtt ggtcaaaaac ttctttgcta ttttcaatta
     1681 cccaaggagc atgtccaatt tccactttag aaatttgctt caatttatcc cagtagtaat
     1741 gactttcgtc ttcttcacct aaataataat aattatatgc taaagcattg tacatttga
//
LOCUS       DAKSQE010000215         1776 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.218, whole genome shotgun sequence.
ACCESSION   DAKSQE010000215 DAKSQE010000000
VERSION     DAKSQE010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.218"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            123..1613
                     /locus_tag="O0V33_002687"
     CDS             123..1613
                     /locus_tag="O0V33_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HCX8977260.1"
                     /translation="MDDKQHTTSSDDERAENATSNQDQQTNSSKRVHLKRWQFISILI
                     GTIIITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQNSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDQALENLV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
BASE COUNT          726 a          227 c          306 g          517 t
ORIGIN      
        1 taaaataaac taagttgtga ctaaaaatta atcaattata gtgaaatatg gtgcgctatc
       61 ttgcataaat tgatatgatt aactacacag aatttaaaag tacataataa ggaagtgata
      121 caatggatga taagcaacac acaacttcat ccgatgatga acgcgctgaa aatgcaacaa
      181 gcaatcaaga ccaacaaact aattcatcga aacgcgttca cttaaaacgt tggcaattca
      241 tatcaatttt aattggtaca atcataataa cagctgtcat cacagttgtt gcatatattt
      301 ttataaatca aaaaataagt ggtttaaaca aaactgatca agcaaactta aataaaattg
      361 aaaatgtgta taaaatctta aatagtgatt attataaaaa acagaactct gacaagttga
      421 gtaaagctgc aattgatggc atggtcaaag aattaaaaga tccttattct gaatatttaa
      481 caaaagaaca aactaaatcc tttaatgaag gtgtttcagg tgattttgta ggtattggtg
      541 cagaaatgca aaagaaaaat gatcaaatta tggttactag ccctatgaag ggttctccag
      601 cagaacgtgc tggcattcgt cctaaagatg tcattactaa agtaaatgga aaatcaatta
      661 aaggaaaagc attagacgaa gttgtcaaag acgttcgcgg taaagaaaac actgaagtaa
      721 ctttaactgt tcaaagaggc agtgaagaaa aagacgttaa gattaaacgt gaaaaaattc
      781 atgttaaaag cgttgagtat aagaaaaaag gtaaggttgg agttattact attaataaat
      841 tccagaatga tacatcaggt gaattgaaag atgcagttct taaagctcac aaagatggtt
      901 tgaaaaagat tgttttagat ttaagaaata atccaggtgg actactagat gaagctgtta
      961 aaatggcaaa tatttttatc gataaaggaa aaactgttgt taaactagaa aaaggtaaag
     1021 atactgaagc aattcaaact tctaatgatg cgttaaaaga agcgaaagac atggatatat
     1081 ccatcttagt gaatgaaggt tcagctagcg cttctgaagt gtttactggt gcgctaaagg
     1141 actataataa agctaaagtt tatgggtcaa aaacattcgg taaaggtgtc gtacaaacta
     1201 caagagagtt taaagatggt tcgttgttaa aatatactga aatgaaatgg ttaacgccag
     1261 atggtcatta tattcacggt aaaggcatta aaccagacgt tactattgac acacctaaat
     1321 atcaatcttt aaatgtcatt cctaatacga aaacatttaa agttggagac gatgataaaa
     1381 atattaaaac tattaaaatt ggtttatcag ctttaggtta taaagttgat aatgaatcaa
     1441 cgcaatttga tcaagcttta gaaaatctag ttaaagcttt ccaacaagcg aataaactcg
     1501 aggtaactgg tgaatttaat aaagaaacga ataataaatt tactgagtta ttagttgaaa
     1561 aagctaataa acatgatgat gttctcgata aattgattaa tattttaaaa taaagcgata
     1621 cacactacta aaattttatt attattatgt taatgacacg cttcctaaat ttgcaaagat
     1681 agcaatttag gaggcgtatt tatttttatt gacgtctaac tctaaaagat ataaattaga
     1741 catttacaaa tgatgtaaat aacacaattt ctatca
//
LOCUS       DAKSQE010000216         1770 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.219, whole genome shotgun sequence.
ACCESSION   DAKSQE010000216 DAKSQE010000000
VERSION     DAKSQE010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.219"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            244..1020
                     /gene="seg"
                     /locus_tag="O0V33_002688"
     CDS             244..1020
                     /gene="seg"
                     /locus_tag="O0V33_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031927817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type G"
                     /protein_id="HCX8977261.1"
                     /translation="MKKLSTVIIILILEIVFHNINYANSQPDPKIDELNKVSDYKSNK
                     GTMGNVMNLYMSPPVEGRGVINSRQFLSHDLIFPIEYKSYNEVKTELENTELANNYKG
                     KKVDIFGVPYFYTCIIPKSEPDINQNFGGCCMYGGLTFNSSENERDKLITVQVTIDNR
                     QSLGFTITTNKNMVTIQELDYKARHWLTKEKKLYEFDGSAFESGYIKFTEKNNTSFWF
                     DLFPKKELVPFVPYKFLNIYGDNKVVDSKSIKMEVFLNTH"
     gene            complement(1595..>1770)
                     /locus_tag="O0V33_002689"
     CDS             complement(1595..>1770)
                     /locus_tag="O0V33_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1829 domain-containing protein"
                     /protein_id="HCX8977262.1"
                     /translation="DIKPNRPSRNKLEPIMLIIVNDVYHPINERAQTVAEHENLEILK
                     WSNKSKIIETLTS"
BASE COUNT          631 a          199 c          278 g          662 t
ORIGIN      
        1 tatatgaggg gacaacgatt tgaatagtag taaaatttga aatttatata ttatcttgaa
       61 tacggattca aaattatttg ttgtaattag taataatgat aaatagaatg ttaatttatt
      121 ttgctagtga atttttattt ttaaaacgaa tgctatcgac acactacaac ctgaactatc
      181 tataagcgtg aatttataat aagtttcatt gtcaaataga ctgaataagt tagaggaggt
      241 tttatgaaga aattatctac tgtaattatt attttgattc tagaaatagt ttttcataat
      301 ataaattatg cgaattccca acccgatcct aaaatagacg aactaaataa agtaagtgat
      361 tataaaagta ataagggaac tatgggaaat gttatgaatc tttatatgtc tccacctgtt
      421 gaaggaagag gagttatcaa ttctagacag tttttatctc atgatttaat atttccaatt
      481 gagtataaga gttataatga ggttaaaact gaattagaaa atacagaatt agctaacaat
      541 tataaaggta aaaaagtaga catttttggt gttccatatt tttatacatg tataatacct
      601 aaatctgaac cggatataaa ccaaaatttt ggaggttgtt gtatgtatgg tggtcttaca
      661 tttaatagtt ctgaaaatga aagagataaa ttaattactg tacaggtaac aatcgacaat
      721 agacaatcac ttggatttac aataactaca aataagaata tggttactat tcaagaacta
      781 gattacaaag caagacactg gctcactaaa gaaaaaaagc tatacgagtt tgatggttct
      841 gcatttgaat ctggatatat aaaatttact gaaaagaaca atacaagttt ttggtttgac
      901 ttatttccta aaaaagaact agtacctttt gttccatata agtttttaaa tatttacgga
      961 gataataaag tagttgattc taagagtatt aaaatggaag tatttcttaa tactcactga
     1021 taagtatatt tgatatcact atacaatcat aaagtgcgtt tttcgaactt ttgttctaag
     1081 tgttttatag attcatgtaa atagttagtt tattgctggt tgaattattt aatttttctc
     1141 aatgatttat tctaaatatg ttttatgtca atagcggttt aaaaatgctg tttaatggcg
     1201 gtttgaaaat gacgtttttt agaggtttaa tattgtcgtg actttaaatg tatttttact
     1261 ttaataatct tttagtcgcc atgattctcc agtggaaatg ataatttaat aacgtatact
     1321 tttgattgtt taagaatgta caatttttta acactctaga gataaatata tcgaggtaat
     1381 ccgccttctc cgttaattta acggaaatac aatgaaatgt tagtattctt aaaagagcaa
     1441 gtgtcaaata cgtatcaaga aaaagatttt ctgattcgtt gaccttgctc tttttgttct
     1501 aaaataaaac gtgcaaataa aaaagtatct acttttttac ttgcacgata tgatttgaaa
     1561 attaaagaat tacaaatagg ggtctaatgt taatttatga ggttaaagtt tcaatgattt
     1621 ttgatttatt agaccatttt aatatctcaa ggttctcatg ttctgctaca gtttgtgctc
     1681 tttcatttat cggatgatat acatcattta caattattaa catgataggt tctaatttgt
     1741 ttctggaagg tctgtttggt ttgatatcac
//
LOCUS       DAKSQE010000217         1732 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.220, whole genome shotgun sequence.
ACCESSION   DAKSQE010000217 DAKSQE010000000
VERSION     DAKSQE010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.220"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            55..795
                     /gene="seh"
                     /locus_tag="O0V33_002690"
     CDS             55..795
                     /gene="seh"
                     /locus_tag="O0V33_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type H"
                     /protein_id="HCX8977263.1"
                     /translation="MINKIKILFSFLALLLSFTSYAKAEDLHDKSELTDLALANAYGQ
                     YNHPFIKENIKSDEISGEKDLIFRNQGDSGNDLRVKFATADLAQKFKNKSVDIYGASF
                     YYKCEKISENISECLYGGTTLNSEKLAQERVIGANVWVDGIQKETELIRTNKKNVTLQ
                     ELDIKIRKILSDKYKIYYKDSEISKGLIEFDMKTPRDYSFDIYDLKGENDYEIDKIYE
                     DNKTLKSDDISHIDVNLYTKKSIINENF"
     gene            819..1540
                     /locus_tag="O0V33_002691"
                     /pseudo
     CDS             819..1540
                     /locus_tag="O0V33_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492129.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
BASE COUNT          717 a          176 c          281 g          558 t
ORIGIN      
        1 gttgtttttg ttgttttata attaagttaa tgaaatatat tgaggagttt tcaaatgatt
       61 aataaaatta aaatattatt ttcgttttta gcattattac tttcattcac atcatatgcg
      121 aaagcagaag atttacacga taaaagtgag ttaacagatt tagctttagc taatgcatat
      181 ggtcaatata atcacccatt cattaaagaa aatattaaga gtgatgaaat aagtggagaa
      241 aaagatttaa tatttagaaa tcaaggtgat agtggtaatg atttgagagt aaagtttgca
      301 actgctgatt tagctcagaa gtttaaaaat aaaagtgtag atatatatgg ggcatctttt
      361 tattataagt gtgaaaaaat aagtgaaaat atttctgaat gtctatatgg aggtacaaca
      421 ctaaatagtg aaaaattggc acaggaaagg gtgattggtg ctaatgtttg ggtagatggc
      481 attcaaaaag aaacagaatt aatacgaaca aataagaaaa atgtgacatt gcaagaatta
      541 gatataaaga tcagaaagat attgtccgat aaatataaaa tttattataa agacagcgaa
      601 ataagtaaag gtctaattga atttgatatg aaaactccta gagattactc attcgacatt
      661 tatgatttaa aaggagaaaa tgactatgag atagataaaa tttatgaaga caataaaact
      721 ttaaaatctg atgatataag tcatattgat gtaaatctat atacgaaaaa aagtataatc
      781 aatgaaaatt tctagatcaa tgttagctgt taattgtcat gtattttttc atttttccgt
      841 tttggaattc atttaagcat ttttctcaag caagtacata tactccaaaa ggagaaactc
      901 taaagaacaa gtccgactta gatttaaccg cgatatataa tattaaatct gtatattcat
      961 aaagtgaatt ttttaaattt taaagataaa attaaaatca aacaactatt aaataaatat
     1021 ttaatgttat aattaagtat tgaaataaaa atagtctaaa acggagctta atagttcaga
     1081 aattgcaaac acattcaaag ggaaaatgta gatatattcg gagtacactt tggagcaaat
     1141 tgtgttggag aagtaggtga gaatagcgct tgtttatatg gtggagtcac atttcacgaa
     1201 aaaatgaaaa acaacaaaaa agtcaatagg cataaatgta tttaaaaatg gatttcaaca
     1261 aaaagggttt gtcattacta ctgataaaat caaacccata gctcaagagc tagatcttaa
     1321 tacgagaaac aatatacaaa atcaatataa aatgtatgat tcggaaactg gagatataca
     1381 aaaaggatat ataaaatttc attctactaa atcaagtttc tattatgact tatttgattt
     1441 taaggggaaa aaacgtgtgg atttcttaaa gttctataat gacaatgaac ttataagtac
     1501 taaaaattta catatagata tctatttgtt taacaagtaa ataattggag acgtttgttg
     1561 ataccataca gccatatggg cagacaaaat gtcagtttat cttttcatca gttgtctaaa
     1621 ctacatggaa aaagtttaat aagcatagga aagattatta acagagagaa gtgacttagt
     1681 ttggaatata aatcttacaa caaaatgagt aataatattt tatctatctt gt
//
LOCUS       DAKSQE010000218         1727 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.221, whole genome shotgun sequence.
ACCESSION   DAKSQE010000218 DAKSQE010000000
VERSION     DAKSQE010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.221"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            130..507
                     /locus_tag="O0V33_002692"
     CDS             130..507
                     /locus_tag="O0V33_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HCX8977264.1"
                     /translation="MSYSIIRVVKVKSKTNTRGIQRENKNYENIDIDLSKSYLNYDLV
                     NDTKFDFNKKIDEKIEKNYKGKRKIRTDAIKHIDGLITSDNVFLINYLKKRQNSFLSM
                     LKRSWKKNTVKKIFYMRQYIWMR"
     gene            447..773
                     /locus_tag="O0V33_002693"
     CDS             447..773
                     /locus_tag="O0V33_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HCX8977265.1"
                     /translation="MEEEYGKENLLYATVHMDEITPHMHYGVIPITKDGRLSAKEVVG
                     NKKALTEFQDRFNTYINKQGYDLKRGISRQLTKEKHDQVSGYKQKTEYHKQMYMREKQ
                     IEDHLK"
     gene            840..1340
                     /locus_tag="O0V33_002694"
     CDS             840..1340
                     /locus_tag="O0V33_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCX8977266.1"
                     /translation="MYEIKTKNVGGWFHKEKQETGNIVITKTYFEKYTKQIKAAQMIL
                     DDYEWIKSGKSLKKSEKQNESLVNELTSVHMENEKLVEEFNDLAQRYNYLLSENEKKD
                     KELNYTLKLFNQVFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKK
                     KYQAQE"
BASE COUNT          715 a          180 c          303 g          529 t
ORIGIN      
        1 gtgatgacaa aaaacaagcc tcgcagagca cacgttttta atgacttatt tggtttttaa
       61 taagtctagt atgttaaaac ttttctgtgt ttaggtgtta taatcatatg cagtggaagt
      121 tgatttatta tgtcttattc aattattcga gttgttaaag tgaaatcaaa aacaaataca
      181 agaggtattc aaagagaaaa taaaaattat gaaaatatag atattgattt aagtaagtca
      241 tatttaaact atgatttagt caatgatact aagtttgatt ttaataaaaa aattgatgag
      301 aaaatcgaaa aaaattataa agggaaacgc aaaattagaa cagatgccat taaacatatt
      361 gatggattaa ttacatcaga taatgtgttt ttaatcaatt atctgaagaa gagacaaaat
      421 agttttttaa gcatgctaaa gcgttcttgg aagaagaata cggtaaagaa aatcttttat
      481 atgcgacagt acatatggat gagataacac cacatatgca ttatggcgta attcctatta
      541 ctaaagatgg acgtttaagt gcgaaagaag ttgtaggtaa taaaaaagca ttaaccgaat
      601 tccaagatag attcaatacg tatattaata agcaaggcta tgatttaaaa cgaggaattt
      661 ctagacaact tacaaaagaa aaacatgatc aagttagcgg ctataaacaa aaaacagaat
      721 atcataaaca aatgtatatg cgcgagaagc aaatagagga tcatttaaag tgagaaaatg
      781 ataaattgat ataaaactac caaaaatcat tagatacgct taaaaagctt ctgagtgtca
      841 tgtatgaaat taaaactaaa aatgtaggtg gatggtttca taaagagaag caagaaacag
      901 gcaatattgt aattactaaa acttattttg aaaaatacac taaacaaatt aaagcagcac
      961 agatgatatt agatgattat gaatggatta aaagtgggaa gtcattaaaa aaatcagaga
     1021 aacaaaatga aagtttagtt aatgaattga ctagtgtcca tatggaaaat gaaaagctgg
     1081 ttgaagaatt taatgattta gcacaacgtt ataattattt gttgtcagag aatgaaaaga
     1141 aagataaaga actaaattat acgcttaagt tattcaatca agtttttaaa atcattaaaa
     1201 gtatgatgaa agaagagcgt tatcatacgc taataaatca tattgataat catttagaca
     1261 attctaaaat acgtgaagta atgacgatag ataacaatga tgaacagttc tttaagaaga
     1321 aatatcaagc acaagaatga gaaatcattt ttaaagaaga tagagaagat ggttatacat
     1381 tataaaaata gatatttaaa tgcatttgtt tgaggttaat tattttttag gatgaaaaat
     1441 aggtagcccc cctctaactt tttaatatat ttttataaat ctttttaaaa acctttttag
     1501 gggcttgttc agacctagat caacaaggga tttcaatgcc taaaggagtg tttttgccac
     1561 ataaaaggag tgttttaaaa agttgtactc cttttatggt ttgacattga taataaagta
     1621 gtttattgtg aataaaagtt ttcaaatggg agtgtatatt caaacgatac tcctttaggt
     1681 tcgtaataaa gggggggctg tatcatgaca ggagagacag ttgtata
//
LOCUS       DAKSQE010000219         1679 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.222, whole genome shotgun sequence.
ACCESSION   DAKSQE010000219 DAKSQE010000000
VERSION     DAKSQE010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.222"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            244..1113
                     /locus_tag="O0V33_002695"
     CDS             244..1113
                     /locus_tag="O0V33_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCX8977267.1"
                     /translation="MEMSRLNLTDNNDINEIIAWRGEQEELEQKRDTYKKRYHEFEME
                     IARLESLTKDKELLDSDKLKDEYELKKGKMNTLIDEYSAVHYQCQNNINKTQSIVSHI
                     NYLNQELKDQQEIFQLAEIVSGKNNKNLTLENFVLIYYLDQIIAQANLRLATMSDNRY
                     QLIRREAVSHGLSGLEIDVFDLHSNKSRHISSLSGGETFQSSLALALGLSEIVQQQSG
                     GISLESIFIDEGFGTLDQETLETALDTLLNLKSTGRMVGIISHVSELKNRIPLVLEVK
                     SDQYQSSTRFKRN"
     gene            complement(1184..1546)
                     /gene="mscL"
                     /locus_tag="O0V33_002696"
     CDS             complement(1184..1546)
                     /gene="mscL"
                     /locus_tag="O0V33_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCX8977268.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
BASE COUNT          659 a          243 c          264 g          513 t
ORIGIN      
        1 tgtcgaaatc tcatcactga atttgaaaca ctctcgaaat ataataatat cactaatttt
       61 gaggtggatt ataagaagta tgtacaagat gtgaatcaac atcaagaaca ctcaaaccag
      121 attgaagata agttaataca attatctcaa agaaagttaa ttgagcaaaa taatctaaat
      181 cactatgaaa aacaactaga aacttacaat aatgacttag aattgaatga acaatctatt
      241 gaaatggaaa tgtcgaggct gaatttaact gataacaatg atataaatga aataatagcc
      301 tggaggggcg agcaagagga attagagcag aaaagggata cttataaaaa acgttatcat
      361 gaatttgaaa tggaaatagc taggttagaa tcattaacca aagataaaga gttattggac
      421 tctgataaat taaaagatga atatgaacta aaaaaaggaa agatgaatac actgatagat
      481 gaatactctg ctgttcatta tcaatgtcaa aataatatta ataaaacaca atctatagtt
      541 tcgcatatta attacttaaa tcaagaatta aaggatcaac aagaaatatt tcaattggct
      601 gagattgtca gtggtaagaa taacaaaaat cttacattgg aaaacttcgt cttaatttac
      661 tatttagatc aaattattgc tcaagcaaat ctgagattag cgacaatgtc agataatcga
      721 taccaactaa ttaggcgaga agcggtttct catggtctta gtggcctaga aattgatgta
      781 tttgatttgc attcaaataa gtctagacat attagctcgt tatcaggtgg agaaactttc
      841 caatcgtcgc ttgcattagc tttagggtta agtgaaattg tacagcagca atcaggaggt
      901 atttcactag aatcaatttt tattgatgaa ggattcggta cattagatca agaaacgctt
      961 gaaacagcgt tagacacttt gttaaatctt aaatcaactg gtagaatggt tgggattatt
     1021 tcacatgtga gcgaattgaa aaatagaata cctttagttt tagaagtgaa atcagatcaa
     1081 tatcagagtt caacaagatt caaaagaaat taaaacataa aaaatagaga tggtactttg
     1141 gaatgtatag aacaacctta gcccatctct ttttatttaa aaattatttt ttctcacgta
     1201 ataaatctct gatttcagtt aataacacaa cattttcttc cacaactgct tcttcttcgg
     1261 cttcttcttt cttcattaag gtatttgcaa tcttaacgaa gatgaataaa gcaaacgcga
     1321 tgataataaa gtcgataaca gattggataa ataaaccata tttaataccc cagaatgacc
     1381 attctttagc aaaatcaact gatccgaaaa ttttaccaat taatggcata atgatatttt
     1441 ctactaatga agatataatc ttgttgaaag ctgcacccat cacaacagca attgctaaat
     1501 ctaagacgtt accttttaag gcgaactctt tgaattcttt taacattaca ctcaacctct
     1561 cttgtttaaa tgtaacctca attatacaac aaataaaaaa attagaaaat agattattta
     1621 agaaaatttg attatcgcaa agacctttgt tatcctataa aggtgcgtga gttattgaa
//
LOCUS       DAKSQE010000220         1646 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.223, whole genome shotgun sequence.
ACCESSION   DAKSQE010000220 DAKSQE010000000
VERSION     DAKSQE010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.223"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..673)
                     /gene="sei"
                     /locus_tag="O0V33_002697"
     CDS             complement(<1..673)
                     /gene="sei"
                     /locus_tag="O0V33_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031927819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type I"
                     /protein_id="HCX8977269.1"
                     /translation="MKKIKYSFILVFILFFNIKDLSYAQGDIGVGNLRNFYTKYDYID
                     LKGVTDKNLPIANQLEFSTGTNDLISESNNWDEISKFKGKKMDIFGIDYNGPCKTKYM
                     YGGATLSGQYLNSARKIPINLWINGKHKTISTDKIATNKKLVTAQEIDVKLRRYLQEE
                     YNIYGHNNNGKGKEYGYKSKFYSGFNKGKVLFHLNDEKSFSYDLFYTGDGLPVSFLKI
                     YEDNKI"
     gene            complement(708..1427)
                     /gene="sem"
                     /locus_tag="O0V33_002698"
     CDS             complement(708..1427)
                     /gene="sem"
                     /locus_tag="O0V33_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_058008129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type M"
                     /protein_id="HCX8977270.1"
                     /translation="MKRILIIVVLLFCYSQNHIATADVGVLNLRNYYGSYPIEDHQNI
                     NPDNNRLSHQLVFSKDNSTVTAEFKNVEDVKKFKNRAVDVYGLSYSGYCLKNKYMYGG
                     VTLAGDYLEKSRCIPINLWVNGNLKTISTDKVSTNKKIVTAQEIDTKLRRYLQEEYNI
                     YGFNDTNKGRNYGTKSKFFSGFNTGKISFHLNDGTSFSYDLFDTGTGQAESFLKIYND
                     NKTVETDKFHLDVEISYKDES"
BASE COUNT          538 a          264 c          186 g          658 t
ORIGIN      
        1 ttattttatt atcttcataa attttcaaaa aacttacagg cagtccatct cctgtataaa
       61 acaaatcata tgaaaatgat ttttcatcat ttaaatgaaa taaaactttc cccttattaa
      121 aacctgaata aaatttagat ttatacccat attctttacc tttaccattg ttattatgac
      181 catatatatt gtattcttct tgaagatatc tccttaattt aacatcaatt tcttgagctg
      241 tgactagttt tttattagtt gctattttgt cagtagaaat tgttttatgc ttgccattaa
      301 tccaaagatt tatagggatt tttctagcag aatttaagta ttgtcctgat aaagtggccc
      361 ctccatacat gtatttagtt ttacaaggac cattataatc aatgccaaaa atatccattt
      421 tctttccttt aaatttactt atttcgtccc aattattaga ttctgagatc aaatcattgg
      481 taccggttga aaattcaagt tgatttgcaa taggtaagtt tttatctgtg acgcctttta
      541 aatctatata atcatatttt gtatagaaat ttcttaagtt acctacacca atatctcctt
      601 gcgcatacga aaggtcttta atgttaaaaa ataatataaa aactaatata aaactgtatt
      661 taattttttt catggcattt cctttactaa gttatgattg aagtatttca actttcgtcc
      721 ttataagata tttctacatc taaatggaat ttatcagttt cgacagtttt gttgtcatta
      781 tatattttta ggaaactttc agcttgccct gttcctgtat caaataagtc ataagagaat
      841 gatgtaccgt cattcaaatg aaatgatatt ttcccagtat taaatccaga aaaaaacttg
      901 gattttgtac cgtaatttct tcctttattt gtatcattaa agccataaat gttatattct
      961 tcttgtagat atcttcttaa tttagtatca atttcttgag ctgttactat ctttttatta
     1021 gttgatactt tgtcagtaga aattgtttta aggttgccat taacccaaag attaatagga
     1081 atacatctag acttctctaa ataatcaccc gctaaagtaa ctcctccgta catatattta
     1141 ttttttagac aatatccact ataacttagg ccgtatacat caacagcacg atttttgaat
     1201 ttttttacat cttcaacatt cttaaattca gctgttactg tcgaattatc cttagaaaaa
     1261 actaattgat gtgaaagacg attattgtca ggattaatat tttgatggtc ttcgattgga
     1321 tagctaccat aatagttcct aagattcaaa actccaacat cagcggttgc gatatgattt
     1381 tgtgaataac aaaacaataa aacaacaatg ataagtattc ttttcatttt atatttctcc
     1441 tataattaaa tatgtctatc tttattatcc ttttattgta aaaaaacatt cttcttctta
     1501 aatcatcctg actctatccc acttatatac tattactcgt taatctactt ttgttatcta
     1561 tactaaaata gaaaaatttt tatagtaata aagttagatt tgaatcatta atcgttcatc
     1621 gtcattcgct atgcaacttt gtaaaa
//
LOCUS       DAKSQE010000221         1611 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.224, whole genome shotgun sequence.
ACCESSION   DAKSQE010000221 DAKSQE010000000
VERSION     DAKSQE010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.224"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(16..1497)
                     /gene="cls"
                     /locus_tag="O0V33_002699"
     CDS             complement(16..1497)
                     /gene="cls"
                     /locus_tag="O0V33_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCX8977271.1"
                     /translation="MQFSFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEY
                     YTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIIIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLA
                     KDLRVAYEHDITKSKQLTEESYANRPLSVKFKESLAKLVSPIL"
BASE COUNT          559 a          284 c          205 g          563 t
ORIGIN      
        1 ctccttacaa ataaattata aaattggcga aactaattta gctaacgatt ctttgaattt
       61 aacagacagc ggtctattgg catatgattc ttcagtaagt tgttttgatt ttgtaatatc
      121 atgttcataa gccaccctta aatctttagc aagattttca tcatatacaa aggcatttac
      181 ttcaaaattt aattcaaaac ttctaaagtc catatttgct gtgcccactg atacgatttc
      241 atcatcaatt aagcacattt tagaatgtat aaatccattt tcatacgtat aaattttaac
      301 accacttgat aataagtcag aggcatttga aaatgtcgcc caataaacta aaggatgatc
      361 tggcttacat ggaatcatta aatgtacatc tacacctgat ttagctgcaa ttttaatggc
      421 atttatatat gaattatccg gaatgaaata aggcgattgc aaatataccg acttcttagc
      481 gctcataatc atttttgtat aaccatattc aatttgatgc cagtcactag ctgggccact
      541 tgcagctatt tgaattggtg aattacccaa tggtccgttc tttttaggga aatacttaac
      601 atcatattca aattgaggtc gatgtgcttg cgaattccag tctaaaataa agcgcaattg
      661 taatgcatca actgcatctc cttgaattcg taaatgtgta tctctccaat atcctagttt
      721 acctaaacca aggtattcat ctccaatgtt aaatcctccg acataaccta attgaccatc
      781 aataataatg atttttctat gatttctgtt attcatacgg aaatttaata atggtagttt
      841 agacgcaaaa aatgcttcaa cttcgccacc taatgattta aagtgatcaa aatttgccat
      901 cttaacattt ttagaaccaa catcatcata taatattttt acttctaaac cttgtttcaa
      961 tttttcttct aaagcatgta aaattctttt acctaaacca tctaaagcga aagtatagta
     1021 ttctaaatgg atatattctt ttgcattttt aatatctttt aaaacttgat cgtataaatc
     1081 attaccgtca atgaaatgat caactttatt attttcagtt aaaaaaccat cttgatccat
     1141 caaaagcata cgtactaaat catgatgttt ttgaacttgt ttgttatcag taccataatt
     1201 acctttatca aagctttcta tttgttgttt caaaagtcca tcgaaatccg ttaacacgtt
     1261 accattgttt ttattcaatt tgcgtgccga aacggttcta ccaaaaaaca agtaaagaat
     1321 aaaaccaatc aagggtaaga caaaaagtac aaatagccat gcccaagttg aactcgctgt
     1381 acgcctattt ctttctaaaa agataataat aaaagctaat actaaattaa tgatgaatcc
     1441 aatggctaaa ataatagtaa ataacgttcc taaatcgttt gaaaatgaaa attgcatagt
     1501 ttatttctcc tataaagaaa ggcacaaaaa acttttgtac ctttcgttcc agattattta
     1561 tttacagata gagttaatat tgcattaaac tcttcttcat tatttggaaa t
//
LOCUS       DAKSQE010000222         1606 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.225, whole genome shotgun sequence.
ACCESSION   DAKSQE010000222 DAKSQE010000000
VERSION     DAKSQE010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.225"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            36..362
                     /locus_tag="O0V33_002700"
     CDS             36..362
                     /locus_tag="O0V33_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HCX8977272.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            364..843
                     /gene="rsbW"
                     /locus_tag="O0V33_002701"
     CDS             364..843
                     /gene="rsbW"
                     /locus_tag="O0V33_002701"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HCX8977273.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKEKNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKDENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            818..1588
                     /gene="sigB"
                     /locus_tag="O0V33_002702"
     CDS             818..1588
                     /gene="sigB"
                     /locus_tag="O0V33_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HCX8977274.1"
                     /translation="MAKESKSANEVSPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHK"
BASE COUNT          609 a          199 c          327 g          471 t
ORIGIN      
        1 agataaatat ttttaataag taggagtgta atgaaatgaa tcttaatata gaaacaacca
       61 ctcaagataa attttacgaa gttaaagtcg gtggagaatt agatgtttat actgtgcctg
      121 aattagaaga ggttttaaca cctatgagac aagatggaac tcgtgatatt tatgttaatt
      181 tagaaaatgt gagttatatg gattcgacag gtttaggttt attcgtaggt acattaaaag
      241 cattaaacca aaatgataaa gagctataca ttttaggtgt gtcagatcgt atcggtagac
      301 tatttgaaat tactggtctt aaggatttaa tgcatgttaa tgaaggaacg gaggtcgaat
      361 aacatgcaat ctaaagaaga ttttatcgaa atgcgcgtgc cagcatcggc agagtatgta
      421 agtttaattc gtttaacact ttctggcgtt ttttcgagag ctggtgctac atatgatgat
      481 attgaagatg ccaagattgc agttagtgaa gctgtgacaa atgcagttaa acatgcatac
      541 aaagaaaaaa acaatgtggg cattattaac atatattttg aaattttaga agataaaatt
      601 aaaattgtta tttctgataa aggtgacagt tttgattatg aaacaactaa atcaaaaata
      661 ggtccttacg ataaagatga aaatatagac tttttacgtg aaggtggcct aggtttattt
      721 ttaatcgaat ctttaatgga tgaagtgaca gtatataaag aatctggtgt gacaatcagt
      781 atgactaagt atataaaaaa agagcaggtg cgaaataatg gcgaaagagt cgaaatcagc
      841 taatgaagtt tcacctgagc aaattaacca atggattaaa gaacaccaag aaaataagaa
      901 tacagatgca caagataagt tagttaaaca ttaccaaaaa ctaattgagt cattggcata
      961 taaatattct aaaggacaat cacatcacga agatttagtt caagttggta tggttggttt
     1021 aataggtgcc ataaatagat tcgatatgtc ctttgaacgg aagtttgaag cctttttagt
     1081 acctactgta atcggtgaaa tcaaaagata tctacgagat aaaacttgga gtgtacatgt
     1141 tccgagacgt attaaagaaa ttggaccaag aatcaaaaaa gtgagcgatg aactaaccgc
     1201 tgaattagag cgttcacctt ctatcagtga aatagctgat cgattagaag tttcagaaga
     1261 agaagtgtta gaagcaatgg aaatgggaca aagttataat gcgttaagtg ttgaccattc
     1321 cattgaagct gataaagatg gttcaactgt tacgctatta gatattatgg gacaacaaga
     1381 tgaccattat gacttaactg aaaaacgtat gattttagaa aaaatattac ctatattatc
     1441 tgatcgcgaa cgagaaatca tacaatgtac gtttattgag ggtttgagtc aaaaagagac
     1501 aggtgagcgt atcggtttaa gtcaaatgca tgtttccaga cttcaaagaa cggcaattaa
     1561 gaaattacaa gaagcagcac ataaatagaa tttgcttatt aataat
//
LOCUS       DAKSQE010000223         1575 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.226, whole genome shotgun sequence.
ACCESSION   DAKSQE010000223 DAKSQE010000000
VERSION     DAKSQE010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.226"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            45..365
                     /locus_tag="O0V33_002703"
     CDS             45..365
                     /locus_tag="O0V33_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCX8977275.1"
                     /translation="MKYIIAILLGLIVSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKDDTPKE
                     VRQE"
     gene            362..1003
                     /locus_tag="O0V33_002704"
     CDS             362..1003
                     /locus_tag="O0V33_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCX8977276.1"
                     /translation="MIELKDLTIQKGNTHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYVYFNGQLEQFKSNFYRDKLGYLFQNYGLIVNLTVNENLDIGLAYK
                     KISKKEKEQLKIRYIEQFGLSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPT
                     GALDPKTGQMIIQSLFDLIDENKVLILATHDMAIANQCDEIIDLEQYRKVASM"
     gene            complement(1091..1381)
                     /locus_tag="O0V33_002705"
     CDS             complement(1091..1381)
                     /locus_tag="O0V33_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977277.1"
                     /translation="MFFACHFKMKIGGVILTRTYNIIGILSCLISFIIMALPMIWYVA
                     SALWFFPGAIMILLLSLVIVFCYIKTKNQLHLLLLVLNIIILFFFSLPLLLS"
BASE COUNT          644 a          188 c          247 g          496 t
ORIGIN      
        1 tatcatggcc gacgttttaa tgaagttatc aaggagtttt aactatgaaa tatatcattg
       61 caatattatt aggattaatc gtatctatta ctattgcttt tacaatcata catcatccta
      121 tacttgatcg ttttaatcct ttcttaaaaa cggaatatag ttatgccaaa gtgccaaaag
      181 gtacgcaaca atatgttaat attacggctt atagtgaaag aggggaaaag cttgattata
      241 aattaacatt taatggattt tctcctagta gaacgtatgt cgaaataaag cataaagggc
      301 aatatgttat atcaatcaca tatgttgaaa aagatgatac accaaaagag gtaagacaag
      361 aatgatagaa ttaaaagatt taaccataca aaaaggtaat acacatattt tgaagaaact
      421 taatttgaaa tttcaatgtg gaaaatcata tgcacttatt ggtaaaagtg gatgcggtaa
      481 atctacatta ttaaatacta ttgctggact tgaaaaaaca ggaaaacaat atgtctattt
      541 taatggtcaa ttagaacaat ttaaatctaa tttttacaga gataaattag ggtatttatt
      601 tcaaaattat ggattaatcg ttaatctgac agtaaatgaa aatttagata ttggattagc
      661 atataaaaaa ataagtaaga aagaaaaaga acaattaaag atacgttata tagaacagtt
      721 tggtctgtca aacagtttaa aaagaaaagt tcatacgcta agtggaggtg aacaacaacg
      781 tgtcgcttta attagaatga tgttaaaaga tccgattgtt atgttagctg atgaaccaac
      841 gggtgcgtta gatcctaaaa caggacagat gattattcaa tcattatttg atttgatcga
      901 tgaaaataaa gtgctgattt tagcaacaca tgatatggct attgcaaatc aatgtgacga
      961 aataatagat ttagaacagt atagaaaagt agcatctatg tgatatccaa aagataaaat
     1021 taaaagccaa tcaattggga atgttaattt caacactcca tttgattggc ttttataatg
     1081 cgttgctatg ttatgaaagt aacaacggca aactgaaaaa aaacaatata ataatattta
     1141 atacgagtag taataaatgt aattgatttt ttgttttaat gtagcaaaaa acaatgacta
     1201 aagatagcag caatatcatg attgcacctg ggaagaacca taatgctgaa gctacatacc
     1261 aaatcattgg taacgccata ataataaaag atataagaca agaaaggata ccaataatat
     1321 tataagttct agtcaaaatt acacctccaa ttttcatttt aaaatgacat gcaaaaaaca
     1381 atcattttaa atacatcata caataaaaat aaattatatg atagatgaat ttgaaattaa
     1441 attgaattgg cttatcattc gtaaacatta ttatgacgta acaattctta taagtggaac
     1501 gagtgtaatt ataccatctg tatagagagt cgtatcaatc gtaaaattta atctatgttg
     1561 ttattgattt ttata
//
LOCUS       DAKSQE010000224         1546 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.227, whole genome shotgun sequence.
ACCESSION   DAKSQE010000224 DAKSQE010000000
VERSION     DAKSQE010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.227"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            1..528
                     /locus_tag="O0V33_002706"
                     /pseudo
     CDS             1..528
                     /locus_tag="O0V33_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            585..>1546
                     /gene="holA"
                     /locus_tag="O0V33_002707"
     CDS             585..>1546
                     /gene="holA"
                     /locus_tag="O0V33_002707"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCX8977278.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKSPKDMAHNVDQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALD
                     LFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYR
                     VKLALGQVRHYQLDELLNIIDACAETDYKLKSSYMDKQLILELF"
BASE COUNT          644 a          166 c          259 g          477 t
ORIGIN      
        1 aagggataaa gtagtaatac attgatgtta ttatcgaaat taagccataa gtataacatt
       61 aaacttatgg atgtaaggca agttagtagt tttaaacttg gagatagtag ttttctattt
      121 tttgatagtt ttattccaaa tagccgagat aaaaatgaat actcgattat tactatgatt
      181 acatatcaaa ataaaaaagt tttattaatg ggcgatgcta gtaaaaataa tgaatcttta
      241 cttctaaaaa aatataattt gccggagatt gatattttaa aagtaggaca tcatgggagt
      301 aagacaagta gttctaaaga atttatagag atgattaagc ctaaaataag tttgatttct
      361 tctggaaaga acaatatgta tcatcttcct aacatagaag ttgttaaacg attgcaaagg
      421 attcgcagtc gcatttacaa tagccaacaa aacggtcaag ttacaataga cttagatgat
      481 aatttaaaag ttgattcaaa ctcttatgga aatgcaagtg gtttatagat acagaaacat
      541 gatataatct attgatgtgt caaacgaatg aaagggtgta ctgaatgagc gacaatattg
      601 tagctattta tggagatgtg cctgaattgg ttgaaaaaca aagtgcagaa atcatatcac
      661 aatttttgaa aagtgataga gatgacttta actttgtgaa atataattta tacgaaacag
      721 agattgcacc aattgttgaa gaaacattaa cattgccttt cttttcagat aaaaaagcaa
      781 ttttggttaa aaatgcatat atatttacag gtgaaaaatc gccaaaagat atggctcata
      841 atgtagatca attaatagaa tttattgaaa aatatgatgg cgaaaatttg attgtctttg
      901 agatatatca aaataaactt gatgaaagaa aaaagttaac taaaactcta aaaaagcatg
      961 caaggcttaa aaaaatagag caaatgtctg aagaagaaat aaaaaaatgg attcaaagta
     1021 aattaaatga gaatttcaaa gatatcaaaa gagatgcatt agatttattt attgagttga
     1081 caggtattaa ctttaatatt gtctcacaag agatagaaaa gttgatttta tttttaggcg
     1141 atagaccaac aattaataag caggatgtta accaaattat taatagaagt ttagaacaaa
     1201 atgtattttt actgactgaa tacattcaga aaagaaagaa agaacaagca attcatttag
     1261 taaaagattt aataacaatg aaagaagaac ctattaaatt acttgcacta attacaagta
     1321 attaccgatt attttatcaa tgtaagattc tgagtcaaaa aggatatagt ggacagcaaa
     1381 ttgctaaaac aataggcgta catccataca gagtaaaatt agcgttagga caagtaagac
     1441 attatcaact tgatgaatta ttgaatatta tagatgcttg tgcggaaact gattataaac
     1501 ttaaatcatc atatatggat aaacagttaa tactggaatt attcat
//
LOCUS       DAKSQE010000225         1517 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.228, whole genome shotgun sequence.
ACCESSION   DAKSQE010000225 DAKSQE010000000
VERSION     DAKSQE010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.228"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            252..1015
                     /locus_tag="O0V33_002708"
                     /pseudo
     CDS             252..1015
                     /locus_tag="O0V33_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
     gene            complement(1063..>1517)
                     /locus_tag="O0V33_002709"
     CDS             complement(1063..>1517)
                     /locus_tag="O0V33_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCX8977279.1"
                     /translation="LLDIASIRSIERGFNYYQSECVINLKSFSETQHETEVKGSGNKV
                     YRCYIDMEHPRKSKCNCPLADGRRVICKHMIALLFTASPEAANKHIIMLNEVEEDYHL
                     RRNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEDEFY"
BASE COUNT          522 a          260 c          211 g          524 t
ORIGIN      
        1 caccaaaacg tgagtattgg tttaatgaaa catagcatga atgtaaattt agtttactga
       61 cataaacacg tcacaaacat acagttatat aagtcccctt atggaatttc atttctagtt
      121 tacataatat atattatagg aagttatatg tgtctaacgc aaaaggtacc ctacatcata
      181 atcattatct aatatcgtca cataacttac ttatgctata atcatggtat tatattgttt
      241 ggagtgattt gatgaaattt gtctttgata ttgacggtac actttgtttt gacggccgat
      301 taattgacca gactattatt gatacattgt tacaattaca acatgctggt catgaactta
      361 tatttgcatc agcacgtccg attcgtgatt tgttgccagt tttaccatca gtatttttat
      421 cagcacacat taattggcgc aaatggtgct atgatttcac agcaatcaat gatttctgtt
      481 atcaaaccaa ttcatacaga tacatatcat catatcttaa aaataattca aaagtatgag
      541 ttagattata ttattgatga tgattggaat tatgctgcac aacttgacgc tgagaacgct
      601 atttttgagc gtttagatcc acataagctg gccagttgta ttgatgttgc aaatatcgac
      661 acaccgatca agattatttt attaaatata gaaccgacac aaattacaac tatattagat
      721 gagctcaata aataccatca agaattggaa atgattcacc attcaaatga atataacatt
      781 gatataacag cgcaaaatat taataaatat actgcattac aatatatatt tgatgcagat
      841 gttaaatata tattgtaatg atcacaatga tattgtcatg ttacaacatg ctagtaatgg
      901 ctatattata ggaccatcag aagcatacac acacgcaata ttgaaacttg ataaagtcaa
      961 acacatcgat aataatgcac tagctatttg caaagtctta aaatcatata aataaaaaca
     1021 cccctatcaa atgataatca ttatcaatgg ataggggcta ttttaataaa attcgtcctc
     1081 ttcatctaat ccaaataatt ctgccatttc tccatgttca attaacatgt ttaaatatgc
     1141 gtcgcggagt tcttcttcac tcatatcatt aatcatttct ttaagagaat caatccacat
     1201 atttctgcgt aagtgatagt cttcttcaac ttcgtttaac atgattatat gtttatttgc
     1261 tgcttctgga ctagctgtaa agagtaatgc aatcatatgt ttacatatca ctcgtcttcc
     1321 atcagcaaga ggacaattac atttggattt tctaggatgt tccatatcaa tataacaacg
     1381 atatactttg ttaccactgc cctttacttc agtctcatgc tgcgtttctg aaaatgattt
     1441 taagttaatt acgcattcac tttgataata attaaagcct ctttctatag aacgaatact
     1501 tgcaatatca agtaatc
//
LOCUS       DAKSQE010000226         1500 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.229, whole genome shotgun sequence.
ACCESSION   DAKSQE010000226 DAKSQE010000000
VERSION     DAKSQE010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.229"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            136..1344
                     /locus_tag="O0V33_002710"
     CDS             136..1344
                     /locus_tag="O0V33_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCX8977280.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLN
                     SLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTN
                     IMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIA
                     AVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLK
                     IHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNRFYGNDVGEY
                     DIKESNKIVE"
BASE COUNT          475 a          196 c          260 g          569 t
ORIGIN      
        1 aattaagtgt attacacggg cgtttcgtta ctgtaaaggg tatttgtatg gtatcattga
       61 tgttagtaat tttacataag tggactgttt ttatacaaca tcttatgaat attttaagtg
      121 gtggtgaatc tataattgaa tgaaaatgaa aagaatataa gaaagaactt tttaaaattt
      181 accgaatcac ggtatatgaa gtttgttggt gggaatgatt tagtcttctc attaatagca
      241 ctagtattgt tgggtattgt tatttttatt ttcgaaaaag tatcatacgt ttttgatcct
      301 tttatcatcg tttttaagac gatagcagca cctatcatcg tctctttaat tctattctat
      361 ctatttaacc caatcgtaaa tatgatggag cgttatagaa taccaagagt tgcaggtatt
      421 tctattattt atctagctgt agtaggtgtt attacgttaa ttgttaattt attgatacct
      481 attattggtt cgcaagtaga tagtttagtt aaaaattcac cgcaatatct agaaaaatta
      541 attaattcta ttgataaaat agcaaataat acgtttttct cttcgtatta tagtcaaatt
      601 aatgattggt taaattcttt acctaagaaa ataccatcta tgttaagtga atttacagat
      661 ggctttgggt ctaaaattgc aacgtttgca gaaacgattg ctaatattgg cgttgtgatt
      721 gtcacaacac catttgtact attctttatg cttaaagatg gacatcactt caaagaattt
      781 tcaacgaata ttatgccacc gaaattccga aaagattttc atgatctact tgaaaaaatg
      841 agtgttcaag ttggttcata cattcaagga caaattatcg tttcattctg tatcggtata
      901 ctgttgttta tcggttattc ggttatcggg ttgaaatata gcttagtatt agctagtatt
      961 gctgcagtta caagtgttgt gccatattta gggcctacta tagcgatttc accagctatt
     1021 gtcatagctg ctataacatc gccgtggatg ctcttaaaat tagcagtagt atggacttta
     1081 gtacaatttg ttgaagggca cttcatttca ccaaatatca tgggtaaaac acttaagatt
     1141 catccactta caatcatttt cattttactg tgtgcaggca aattgcttgg tattgtaggc
     1201 gttattttag gtattccggg atatgctatt ttaaaagtat tagttactca tttattccaa
     1261 ttatttaaac gtcgatacaa tcgtttctat ggtaatgatg taggtgaata tgatattaaa
     1321 gaaagtaata aaatagttga ataagtaatt aaaaatacca atagactata cgtttgttgg
     1381 tatttttatg gtgcttataa tgttaggtgt tcaaatttat gattatcagc acatgtttag
     1441 cttataaatt tttatgaaag tttagccatt tataaagcac atgcataatg aagcgagtat
//
LOCUS       DAKSQE010000227         1499 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.230, whole genome shotgun sequence.
ACCESSION   DAKSQE010000227 DAKSQE010000000
VERSION     DAKSQE010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.230"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..958
                     /locus_tag="O0V33_002711"
     CDS             <1..958
                     /locus_tag="O0V33_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCX8977281.1"
                     /translation="KLRIEIVKYMKRRGVDCNINNICITSGAVQGLKLIADGLLIPQS
                     KIIIETPSYINSIRTWHNIRAKIIPLSINYIKQNINNIFKLNSDYRHSIFYCIPTLHN
                     PTQNTYSKEEKQKIIDQCHKEGIPIVEDSIYSDLWFSPNQQISMKALKNSDNVLYLGS
                     LSKTVSPGLRIGWIIADEKVIHHLADLKMQNDYGASSISQYIATTWLSNYHEDHLYKL
                     KIELKLRKSIFIKSLKKHLSKFGYWNEPQGSFYIWFKLSVPVNIKLLFNEAIKENILI
                     HPGEIYDINSKSYIRFSYSYIDKEEIDQSLKKLSEIINRIRT"
BASE COUNT          583 a          224 c          189 g          503 t
ORIGIN      
        1 aaagttaagg atagaaatag taaagtatat gaagcgaaga ggagttgatt gtaatatcaa
       61 taatatatgt attacatccg gtgctgtaca aggtttaaaa ctaattgcag acggactgtt
      121 aattccacaa tctaaaatta taattgaaac accatcatac attaattcca tacgtacttg
      181 gcataatatc agagcgaaaa ttattccttt gtccatcaat tacatcaaac agaatattaa
      241 caatatcttt aaattaaata gtgattatag gcacagtatt ttttactgta tacctacgct
      301 gcataaccct actcaaaata cttatagtaa agaagagaag caaaaaatta tagatcaatg
      361 tcataaagaa ggtattccaa ttgtggaaga tagtatatac tctgatttat ggttcagccc
      421 taaccaacaa atttctatga aagctttaaa aaatagtgat aatgttttat atttaggtag
      481 tttatctaaa actgtaagtc ccggtttacg tattggttgg attatagcag atgaaaaagt
      541 aattcatcat ttagccgatt taaaaatgca aaatgattat ggtgcaagtt ctatatcaca
      601 gtatattgcc acaacgtggt taagtaatta tcatgaagat catttatata aattaaaaat
      661 cgaactaaaa ttacgaaaat ctatttttat aaagtcatta aagaaacatc tatctaagtt
      721 tggatattgg aatgaacccc aaggctcatt ttatatctgg tttaagctat ctgttccagt
      781 aaatataaag ctcttattta acgaagcaat aaaagaaaat atactaatac acccaggaga
      841 aatatatgac attaactcta aaagctatat ccgcttttca tactcatata tcgacaagga
      901 agaaattgac caaagcttaa aaaaattaag tgaaattata aatagaatac gaacttagtt
      961 aacatttatc accctttata taattcattt tatacatgcg tgttataata ttaagcacaa
     1021 tacgcattac atttcataaa aaacctaaaa tgcattttgt tataagatat tgggatatta
     1081 tacacattta caaatattga caatatgatc tatatcatca aatattattt cattgtatga
     1141 atatagaagt tttattaaaa gataataatg agtactatta aaaagggaat tccccaaaaa
     1201 atgttttgat tggaaattcc cttttttaat ttttatgtgg cgaattgttg cacttaattt
     1261 gacaacctaa aacttaagaa aatattcagt cacacaacct atcttactta ttacttattt
     1321 aattccgtat acccatctca caaatccatc tataatacaa actatcattt tatataagca
     1381 gaatttaccc tgtcttccac acaaactgtt ttcgtcactt taatatgtct tctattcaaa
     1441 aaagtcactc ataacaaatt gcgcatttta ataaatttag caatgccatt tgcatgctg
//
LOCUS       DAKSQE010000228         1488 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.231, whole genome shotgun sequence.
ACCESSION   DAKSQE010000228 DAKSQE010000000
VERSION     DAKSQE010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.231"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            278..1237
                     /gene="hyl"
                     /locus_tag="O0V33_002712"
                     /pseudo
     CDS             278..1237
                     /gene="hyl"
                     /locus_tag="O0V33_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
BASE COUNT          571 a          229 c          233 g          455 t
ORIGIN      
        1 ataaaattag ctttgtcttt tccttggttc ataaagagtt cattcaactt tgactaagcc
       61 tcgacattga aatgcttcct ttcaaatatt aattaaaagt taaaaacata ttcttaaaat
      121 attcactcat catcactcaa tcatttaaca gttgctacat taaatatact aatataaacg
      181 atgataaata tttgatatgt ctcaactgca ttattctaaa ttgacatatt gattattgtt
      241 tcctcaaatc gttttaaaaa atagaaggat gatgaaaatg aaaacacgta tagtcagctc
      301 agtaacaaca acactattgc taggttctat attaatgaat cctgtcgcta atgccgcaga
      361 ttctgatatt aatattaaaa ccggtactac agatatagga agcaatacta cagtaaaaac
      421 aggtgattta gtcacttatg ataaagaaaa tggcatgcac aaaaaagtat tttatagttt
      481 tatcgatgat aaaaatcaca ataaaaaaat attagtcatc agaacgaaag gtaccattgc
      541 tggtcagtat agagtttata gcgaagaagg cgctaataaa agcggtttag cctggccttc
      601 agcatttaag gtatagttgc aactacctga taatgaagta gcacaaattt ctgattacta
      661 ccctagaaat tcaattgata ctaaagaata tatgagtaca ttaacttatg gattcaacgg
      721 taacgtgact ggtgatgatt ctggaaaaat tggcggtttg attggtgcaa atgtttcaat
      781 tggtcataca ctcaaatatg ttcaaccaga tttcaaaaca attttagaaa gtccaactga
      841 taaaaaagta ggttggaaag ttatatttaa caatatggtg aatcaaaatt ggggaccata
      901 tgacagagat tcttggaacc cagtatatgg caatcaactt ttcatgaaaa caagaaacgg
      961 ttcaatgaaa gcagcagaga acttccttga ccctaacaaa gcaagttctt tattatcttc
     1021 aggattctca ccagactttg ctacagttat tactatggat aggaaagcaa ccaaacaaca
     1081 aacaaatata gatgtaatat acgaacgagt tcgtgatgac tatcaactac attggacttc
     1141 aacaaattgg aaaggtacca atactaaaga taaatggaca gatcgttctt cagaaagata
     1201 taaaatcgat tgggaaaaag aagaaatgac aaattaatgg ttatgtacac tcaaatagtc
     1261 acataataat attagccgat aacatcagaa atgatgtgcg tcggctattt tagttgcagt
     1321 ataaaattaa acatcatgaa tagtaatata cctattatgc ttttcagtat atattcacgg
     1381 ctaaaatcat gattacggta ctgatttctt aactacaaac atttgaaatt cattgttgtc
     1441 aaacccttta attaaaagtt ttgaaattgt tcaaaactta ttgaacac
//
LOCUS       DAKSQE010000229         1457 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.232, whole genome shotgun sequence.
ACCESSION   DAKSQE010000229 DAKSQE010000000
VERSION     DAKSQE010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.232"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..474)
                     /locus_tag="O0V33_002713"
     CDS             complement(<1..474)
                     /locus_tag="O0V33_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HCX8977282.1"
                     /translation="MLKKAKLILIATLLLSGCSTTNNESNKETKSVPEEMEASKYVGQ
                     GFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFSLKVKATNVVGSGDGVEVYVHCDDH
                     DIVFNASIPFDKSIIDSDSSLRSEDKGDDMSTLVGTVLSGFEYRAQKEKYDNLYKF"
     gene            complement(564..779)
                     /locus_tag="O0V33_002714"
     CDS             complement(564..779)
                     /locus_tag="O0V33_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977283.1"
                     /translation="MTTLPSHYDDMFILNTQAHARLDQMAQQFEVVCNGLNENEGQAI
                     QTMDQSASLIRSNLIQVKEQLEKLAVY"
     gene            complement(1263..>1457)
                     /locus_tag="O0V33_002715"
     CDS             complement(1263..>1457)
                     /locus_tag="O0V33_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCX8977284.1"
                     /translation="GLGATSATRYGDNRTNYRFDVGQAVYAPGTLIYVFEIIDGWCRI
                     YWNNHNEWIWHERLIVKEVF"
BASE COUNT          449 a          279 c          220 g          509 t
ORIGIN      
        1 aaatttatat aaattgtcat acttttcttt ttgtgctcga tattcaaagc cactcaacac
       61 tgtaccaact aaagtactca tatcatcgcc tttatcctca cttcttaatg agctatcact
      121 gtcaataatt gatttgtcaa atggaatact cgcattaaat acgatatcgt ggtcatcaca
      181 atgcacgtat acttctacgc cgtcgccact acctacaaca ttcgtagctt taacttttag
      241 actgaagtta tccataaaaa attgttcgcc tcgtttggca attttgtctt tatgcttttt
      301 cgcaaattca atcgcatctt tttctgcagg tggttggaag ccttgtccta catattttga
      361 agcttccatt tcttctggta cagattttgt ttctttatta gattcgttat tggtagttga
      421 acatcctgat agcagtagcg ttgctattaa gattaatttt gcctttttaa gcatatttca
      481 tcacctattt atgtgtttgt aaaactttta tgtttgaaaa agctacttat tctcaatgaa
      541 aacaagtagc ttttaataaa taattagtat actgctagtt tttctaattg ttctttaact
      601 tgaattaagt ttgaacgaat taacgaagca gattgatcca ttgtttgaat agcttgccct
      661 tcattttcgt tcaagccatt acaaacaact tcaaactgtt gtgccatttg atcaagacgt
      721 gcatgagctt gagtgtttaa aataaacata tcatcataat gagatggtaa agtggtcatt
      781 ctctaacgta cacgagcgtc caaaaacact gttgtatcaa ggtttttgtc atttttaacc
      841 ttttagattt tccacaagcg tttgcccttt ttcaaataat ctgccctttt ttcgccccga
      901 aaaaacgcaa aaaataacca caatcctaaa ttaataggat ctgtggtttt gttggttgta
      961 ggggaataaa tataaccgta tcgattaaga tacggttgta gcgaatgtaa catttctatg
     1021 ttgttaagat atatgtatcg agtgatgaca aggaagatgt ctcctgtggg accaacagtc
     1081 agatacatgg cctctgccgg gctatatagt tcactcctac tatatacgca tgtaattata
     1141 acataaaaaa aataggcaag taccgaagta cctgcctgtt atgcacattt aaatcttgag
     1201 agtaatgtta tctaaagaat aatgttatta tatcacaata caacatttaa gcaaacatta
     1261 tattaaaaca cttctttcac aatcaatctc tcatgccata tccactcatt atgattgttc
     1321 caataaatgc gacaccaacc atctataatt tcaaacacat atattaatgt tccaggcgcg
     1381 tatacagcct gtccaacatc gaatctatag ttagtacgat tatcaccgta tctagtggct
     1441 gaagtagcac ctaagcc
//
LOCUS       DAKSQE010000230         1430 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.233, whole genome shotgun sequence.
ACCESSION   DAKSQE010000230 DAKSQE010000000
VERSION     DAKSQE010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.233"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            63..1409
                     /locus_tag="O0V33_002716"
     CDS             63..1409
                     /locus_tag="O0V33_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HCX8977285.1"
                     /translation="MKKEILKKQWVLWTILVTSFFSIGTPDIAIIPFSLSIYALSKLI
                     LVNKFVEPDLVTLQNLKEKNKSINIENQKIKREIQELKNLKKDLISSIEEGTKELEHI
                     TSYLNDELFKYDIELTYPFDLVEVDSSQINTYIKKLQMKEKELLNLEEVKIFNVSTEN
                     KRHQNAQAKQIIRLFNAETSQLINKVNSKNIESMQNKIFKSYEGINKIFETDNVRIPE
                     TLLDIKLEMLDLMHKYQVKIEDEKIIRREERARLKEIEQAEKEMEKKLKELDKDIRHH
                     NNEIKKLTMYLNNTDLQVEKELYIEKIRELDQSLKNLNSERENVEDRKDNAQSGFVYI
                     ISNIGSFGENVYKIGVTRRLEPMDRINELSSASVPFEFDVHALIFSENAFELKNKLHE
                     YFKKYKVNKVNGRKEFFKVNINEIKDKVLSEHNSTVQFIDEPKAIQYRETLRLTSL"
BASE COUNT          649 a          168 c          214 g          399 t
ORIGIN      
        1 aagcaattaa gccatcgaga gtatttaaat acatataaat tcaaattaag gagaataggt
       61 aaatgaaaaa agaaatttta aaaaagcaat gggtattatg gacaatctta gtaacatctt
      121 tctttagtat aggcacacca gatatagcaa taatcccgtt cagtttatca atatatgcat
      181 tatctaaatt aatacttgtt aataaatttg tagaacccga tttagtaaca cttcaaaact
      241 tgaaagaaaa aaataaaagt atcaacattg aaaatcaaaa aatcaagcgt gaaatacaag
      301 aattgaagaa tctaaaaaaa gatttaatat ctagtataga agaaggtact aaagaacttg
      361 aacacattac tagttattta aatgatgaat tatttaaata tgatatagaa ttaacttatc
      421 ccttcgattt agttgaagta gactcatctc aaattaatac atatattaaa aaattacaaa
      481 tgaaagaaaa agaattacta aatcttgaag aagtgaaaat atttaatgtt tctacagaga
      541 ataagagaca tcaaaatgca caggccaagc aaataatacg attatttaat gctgaaactt
      601 ctcaattaat aaataaagta aatagcaaaa atatcgaaag catgcagaac aaaatattta
      661 aaagttatga agggataaat aaaatatttg aaacggataa tgtacgtatt cctgaaactt
      721 tacttgacat aaaacttgag atgttagatt taatgcacaa gtatcaagtt aagatagaag
      781 atgaaaagat cataagaaga gaagaacgag ctagactgaa agaaatagaa caagcagaaa
      841 aagaaatgga gaaaaaacta aaagagcttg ataaagacat tagacaccac aataatgaaa
      901 tcaaaaagct gacaatgtat ttaaataaca cagatttaca agtcgaaaaa gaattatata
      961 ttgaaaagat tagagaatta gatcaatcac tcaagaattt aaattctgaa agagaaaatg
     1021 tagaggacag aaaagataat gctcaatcag ggtttgttta tataatatca aatatcggtt
     1081 catttggaga aaatgtttat aagattggtg taacccgtag attagaacca atggatagaa
     1141 ttaatgaatt aagtagcgca tctgttccct ttgaattcga tgtgcacgct ttaattttct
     1201 ctgaaaatgc ttttgagctt aaaaacaaat tacacgaata ctttaaaaaa tataaagtta
     1261 ataaagttaa cggaagaaaa gaattcttta aggttaatat taatgaaatt aaagataaag
     1321 ttttatcaga acacaatagc acagttcaat tcattgatga accaaaagct atacaataca
     1381 gagaaacttt aagattaact tcactctaaa aatttgggta gcccgcctac
//
LOCUS       DAKSQE010000231         1410 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.234, whole genome shotgun sequence.
ACCESSION   DAKSQE010000231 DAKSQE010000000
VERSION     DAKSQE010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.234"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            164..670
                     /locus_tag="O0V33_002717"
     CDS             164..670
                     /locus_tag="O0V33_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977286.1"
                     /translation="MIIKILTILLLLCILSYLVTNKKKPFLFLKTLFMGVVFIFIGYI
                     SLAISAVIIYGIIQFITIDFGSFFLMGIILILISSIFQLFIVRLLFRKKNVDLTEVVV
                     LEHLIQWFLVYFAIYQAVNEKMDINDINIDNFQSVFFDVSNLNLVILPTLIISWVTIF
                     NYRMRSYK"
     gene            927..>1410
                     /locus_tag="O0V33_002718"
     CDS             927..>1410
                     /locus_tag="O0V33_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding protein"
                     /protein_id="HCX8977287.1"
                     /translation="MKNKLIAKSLLAIAAIGITTTTIASTADASEGYGPREKKPVSIN
                     HNIVEYNDGTFKYQSRPKFNTTPKYIKFRHDYNIVEYNDGTFEYGARPQFNKPAAKTE
                     ATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYNVKKMILQERIDQVLK
                     Q"
BASE COUNT          535 a          171 c          224 g          480 t
ORIGIN      
        1 agcataattc aacacttaca tttttaaata gataaaatag tgtaaaatca cgtgccaaga
       61 ttactataga gtaggcattg agtattattg gcaatcactt aagtgattgt tattttttcg
      121 tggtcaataa taaagatata aataaacata ttgaggtcaa acgatgataa ttaaaatttt
      181 aacaattcta ttactacttt gtatattgag ctatttggtt acaaataaaa agaagccttt
      241 tctgttctta aagacactct ttatgggtgt ggtgtttatc tttataggat atatttcact
      301 ggcaatatct gccgtaatta tttatggtat tattcaattt atcacaattg attttggtag
      361 ttttttctta atgggtatta ttttgatctt gatttcaagt atattccaat tatttatagt
      421 tagattactt tttagaaaaa agaatgtcga tttaacagag gttgtcgttt tagaacattt
      481 aattcaatgg ttcttagttt attttgcgat ctatcaagca gtaaatgaaa aaatggacat
      541 taatgatatt aatatcgaca atttccaatc agtctttttt gacgtgtcta atttgaattt
      601 agtaattcta ccaactttga ttattagctg ggttacaata tttaactata gaatgagaag
      661 ttacaaataa aatctatgaa attataccat cagacaccaa cattcaaatg gtgtcttttt
      721 gttgtgtagt tttatttttg aaattcgaaa agtagaggca tgaaattttt gactagtgta
      781 taagtattga tgagacacaa gatagataac tatattttgt ctatattata aagtgtttat
      841 agttaattaa taactagtta atttcaaaag ttgtataaat aggataactt aataaatgta
      901 agataataat ttggaggata attaacatga aaaataaatt gatagcaaaa tcattactag
      961 cgatagcggc aattggtatt actacaacta caattgcgtc aactgcagat gcaagcgaag
     1021 gatacggtcc aagagaaaag aaaccagtga gtattaatca caatatcgta gagtacaatg
     1081 atggtacttt taaatatcaa tctagaccaa aatttaatac aacacctaaa tatattaagt
     1141 ttagacatga ttataacatc gtggaatata atgacggtac attcgaatat ggtgcacgtc
     1201 cacaatttaa taaaccagca gcgaaaactg aggcaactat taaaaaagaa caaaaattga
     1261 ttcaagccca aaatcttgtg agagaatttg aaaaaacaca tactgtgagc gcacatagaa
     1321 aagcacaaaa ggcagtcaac ttagtatcat ttgaatacaa tgttaagaaa atgatattgc
     1381 aagagcgaat tgatcaagta ttaaagcaag
//
LOCUS       DAKSQE010000232         1377 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.235, whole genome shotgun sequence.
ACCESSION   DAKSQE010000232 DAKSQE010000000
VERSION     DAKSQE010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.235"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..229)
                     /locus_tag="O0V33_002719"
     CDS             complement(<1..229)
                     /locus_tag="O0V33_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001798254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977288.1"
                     /translation="MNQMLLNNQEFVEALTIKERIELFKEMEEDYFNYDQEIIDSIFM
                     EWINRKSSLKMEDFDEIITNQGYDYRYYKYVI"
     gene            complement(297..530)
                     /locus_tag="O0V33_002720"
     CDS             complement(297..530)
                     /locus_tag="O0V33_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001798253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lichenicidin A2 family type 2 lantibiotic"
                     /protein_id="HCX8977289.1"
                     /translation="MKKEGSMQMKTENIIGKSFVELDQAEMDFISGAEGTVEPQATPT
                     ISSVACIRVSVGVSAAGASFVTSFVASATSNCD"
     gene            complement(533..745)
                     /locus_tag="O0V33_002721"
     CDS             complement(533..745)
                     /locus_tag="O0V33_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lichenicidin A2 family type 2 lantibiotic"
                     /protein_id="HCX8977290.1"
                     /translation="MSNELNNLVGLSFTELNQEEMDFISGAEGTVEPQATPTIASPFT
                     PYAIEISIGATVSAVSGLVSYTKDCL"
     gene            847..>1377
                     /locus_tag="O0V33_002722"
     CDS             847..>1377
                     /locus_tag="O0V33_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HCX8977291.1"
                     /translation="MKLIEQNEHSECGIACITMILSYYKSNITLADIRDKFGVPKGGH
                     SLANMKEILNYYGLNSKGFRVDDVDDLNQLQSPVICHWNNSHFILLKKVKRNNYYIFD
                     PDLGKRKLNREEFLEGFSNSILIIEGKNSKFDTRKTRSSLSKKYIFNNQIHLIFSIFI
                     FSFIMQIIGLLVPLATQ"
BASE COUNT          467 a          218 c          192 g          500 t
ORIGIN      
        1 atattacata tttataatac ctgtaatcat atccttgatt agttattatt tcatcaaaat
       61 cttccatttt taatgaactt tttctattta tccattccat aaaaatacta tcaatgattt
      121 cttgatcgta attaaaataa tcctcttcca tttctttaaa taattcaatt cgttccttta
      181 tagtcaaagc ttctacaaat tcttgattat tcagtaacat ttgattcaca ccctaataga
      241 ttaattgtta aataaagggg gagatttccc ccttaccttt atgccaaaat tcttaattaa
      301 tcacaatttg atgttgctga agctacaaaa gaagtaacaa aagatgcccc agctgcggat
      361 actcctacgc taactctaat acatgcaact gaagaaattg ttggtgtagc ttgtggctct
      421 acagtaccct ctgcaccaga aatgaaatcc atttccgctt gatctagttc tacgaatgat
      481 tttcctataa tgttttcagt tttcatttgc atactaccct cttttttcat aattataaac
      541 aatcttttgt atagcttact aaacctgata ctgcagatac tgtagcgcca attgaaattt
      601 ctattgcata tggcgtaaat ggtgatgcaa ttgttggagt ggcttgtggt tccactgttc
      661 cttcggcacc agaaataaag tccatttctt cttggtttaa ttctgtaaac gagagaccaa
      721 ctaaattatt aagttcgttt gacatattta cacctccttt tagaatataa tgataatgat
      781 tttatataat atatattgtc tatatattat aaactatacc taaaattgtg acttgcaggt
      841 gctgaaatga aattgattga gcaaaatgag catagcgaat gtggtattgc atgtattact
      901 atgattcttt catattataa atccaacatt accctcgctg atattagaga taaatttggt
      961 gttccaaaag gtggacattc attagctaat atgaaagaaa tcctaaacta ttatgggtta
     1021 aattctaaag gctttcgagt agatgatgta gatgacttaa atcaattaca aagtccagta
     1081 atatgtcatt ggaacaattc acattttatt cttctaaaaa aagtaaaaag aaataattat
     1141 tacatttttg atccagattt aggcaagaga aagttgaata gagaagaatt tttagaaggt
     1201 ttctcaaatt caattctaat tattgaggga aaaaattcaa aatttgatac tcggaaaact
     1261 agaagtagcc tttcaaagaa gtatattttt aacaaccaaa ttcatcttat atttagtata
     1321 tttatattta gctttattat gcaaataata ggtttattag tgcctttagc tacacag
//
LOCUS       DAKSQE010000233         1301 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.236, whole genome shotgun sequence.
ACCESSION   DAKSQE010000233 DAKSQE010000000
VERSION     DAKSQE010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.236"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..255
                     /locus_tag="O0V33_002723"
     CDS             <1..255
                     /locus_tag="O0V33_002723"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCX8977292.1"
                     /translation="VDKKGIYYPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYD
                     RFLANYQTKTISLLYDFQITSFEPESFDQPVDKLIIYQSA"
     gene            267..>1301
                     /locus_tag="O0V33_002724"
     CDS             267..>1301
                     /locus_tag="O0V33_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCX8977293.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSTIR
                     IKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPAT
                     YTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHI
                     LMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLI
                     GSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGY
                     YYKVNRNIFWI"
BASE COUNT          458 a          151 c          232 g          460 t
ORIGIN      
        1 gtagataaga aggggattta ctatccaact tcaaaaggtg aaacaacgaa taacctagat
       61 ttaattgttg ttcctggtgt tggatttcaa gacgatggat atagaattgg gtatggtggt
      121 ggctattatg acaggttttt agctaattat cagacaaaga caataagctt attatacgat
      181 tttcaaataa catcatttga accggaatca ttcgatcaac cagtcgataa attgattata
      241 tatcaatcag catagtggag gactaaatga acatagacaa acaattttgg aaaacaatat
      301 attattggat taggtacttg aattttgata ttgtcagtag ggaaaaagat gaccaagaaa
      361 tttggttagc ccataaaaga aaaaaacaag tagtcatttt taaacaacat atcaagtcta
      421 cccaagaaat acgttttgac aaagcgaaag tgcttgaaca caaagatgaa atagcaaatt
      481 ttatttcttt cgaaccacaa agttttgaat tttattattt tacagaatcg gaattttcag
      541 aagaacaatt aaatgaagtt tcgacaatta gaattaaatt caatgttata agacacacaa
      601 aagatttgat aaagcatatg ccgaatatat ttttggctag acttatttca gaagataatg
      661 ataaaaagac atatatgttc tataaacgca aagtattaac cgataacttt ttagataaat
      721 atatgcagaa attttcaccg gcaacataca caataatatt tgtaaatgtc ttaatatggt
      781 tatgtatgat tttatattta aataattttt cggatgtaaa attattagat gttggcgggt
      841 tagtgcattt taatgtcgta catggtgagt ggtatcgaat tgttacatcg atgtttttac
      901 attttagttt tgaacatata cttatgaata tgctttcatt atttattttt ggtaaaatag
      961 tcgaagcaat tattggttca tggaggatgt taactgtata ctttattgca gggctgtttg
     1021 gaaactttgt atcactatca tttaatacga ctacaatttc agttggggct agtggtgcta
     1081 tattcggtct gatcggatca atttttgcga tgatgtatgt ttcaaaaaca tttaacaaaa
     1141 aaatgttagg acagttatta attgcattag tgatattagt tggtgtttct ctgtttatgt
     1201 caaatataaa tattgtggcg catattggag gcttcattgg tggtttatta ataactttaa
     1261 ttggctatta ctataaagtg aatcgtaata ttttttggat t
//
LOCUS       DAKSQE010000234         1300 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.237, whole genome shotgun sequence.
ACCESSION   DAKSQE010000234 DAKSQE010000000
VERSION     DAKSQE010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.237"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(143..1234)
                     /locus_tag="O0V33_002725"
     CDS             complement(143..1234)
                     /locus_tag="O0V33_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HCX8977294.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLSDLYNAKKLK"
BASE COUNT          400 a          239 c          186 g          475 t
ORIGIN      
        1 tttatttcta tacaaaaaat aatagccact ctaaaagtaa tatactctta gaatggctac
       61 aagtttaaaa ttgtcgattc aaagataata ttctacttca acatgactta ttagttaagt
      121 agtcaatagt ttccttggaa tattatttta actttttagc gttatataaa tcagacaaag
      181 gctttaattc atcataaaat gcttggaatt catctaaatc catttgttga cccgcatcac
      241 taagtgcaac agatggatct ggatgcacct cagccataac gccatcagca cctactgcta
      301 atgctgcttt cgcagttggt aacatgatat ctttacgacc tgtactatgc gtaacatcta
      361 ccatgactgg taagtgtgta ccttgtttta aaattggtac tgctgaaata tctaaagtgt
      421 tacgtgtcgc cttttcataa gttcggattc cacgttcaca taaaataatg ttttgattac
      481 cttgtgaagc aatgtattca gctgcataaa caaactcttc gattgtagca gataaaccac
      541 gttttaatag aataggcttt ttcgtacggc cagcttcttt taataactcg aagttttgca
      601 tattacgtgc accaatttgg aatacgtcta aatactcatc agccacttca aaatcatttg
      661 gatttacgat ttcgctgaca acatttaaat catatttatc tttaatttgt ttaagtattt
      721 taagaccttc gacacctaaa ccttggaagt catatggtga tgtacgtggc ttaaatgcac
      781 cgccacgaat aaatttttca cctttagcat gtaagttttt agcaacagct tcaacttgtt
      841 caaatgattc aactgaacat ggcccaaata caaatgattt attgccgtct ccaataatgc
      901 ccccattatc aaatgttaca atcgtatctt caggtttcaa cttacgtgat acatataaat
      961 gtttttcatt ttcagatttt tgtaaatctg tagaggcttt gaaaatttct ttaaataatt
     1021 gcttaatagt attatcgttg aatggtcctt tgttactatc gattaagtcg ttaagcattt
     1081 ctttttcgcg ttgtggatca tagatacgtg taccttgttt taatttttct tccccaattt
     1141 tttgtgctag ttcaccacgt ttagataata agtctaaaat ttgatgattc agtgagacaa
     1201 tctcacttct gtatgattct aatttattac tcatccttac tcacctctac gaattatttc
     1261 aagtatattc cactttaaag caataaaata taatgttaaa
//
LOCUS       DAKSQE010000235         1288 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.238, whole genome shotgun sequence.
ACCESSION   DAKSQE010000235 DAKSQE010000000
VERSION     DAKSQE010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.238"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(215..298)
                     /locus_tag="O0V33_002726"
     tRNA            complement(215..298)
                     /locus_tag="O0V33_002726"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Leu,seq:caa)
     gene            complement(427..501)
                     /locus_tag="O0V33_002727"
     tRNA            complement(427..501)
                     /locus_tag="O0V33_002727"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Gly,seq:tcc)
     gene            complement(509..582)
                     /locus_tag="O0V33_002728"
     tRNA            complement(509..582)
                     /locus_tag="O0V33_002728"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(548..550),aa:Cys,seq:gca)
     gene            complement(588..659)
                     /locus_tag="O0V33_002729"
     tRNA            complement(588..659)
                     /locus_tag="O0V33_002729"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(625..627),aa:Gln,seq:ttg)
     gene            complement(671..743)
                     /locus_tag="O0V33_002730"
     tRNA            complement(671..743)
                     /locus_tag="O0V33_002730"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(708..710),aa:His,seq:gtg)
     gene            complement(746..819)
                     /locus_tag="O0V33_002731"
     tRNA            complement(746..819)
                     /locus_tag="O0V33_002731"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(785..787),aa:Trp,seq:cca)
     gene            complement(835..915)
                     /locus_tag="O0V33_002732"
     tRNA            complement(835..915)
                     /locus_tag="O0V33_002732"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(879..881),aa:Tyr,seq:gta)
     gene            complement(921..996)
                     /locus_tag="O0V33_002733"
     tRNA            complement(921..996)
                     /locus_tag="O0V33_002733"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(961..963),aa:Thr,seq:tgt)
     gene            complement(1004..1076)
                     /locus_tag="O0V33_002734"
     tRNA            complement(1004..1076)
                     /locus_tag="O0V33_002734"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1041..1043),aa:Phe,seq:gaa)
     gene            complement(1095..1170)
                     /locus_tag="O0V33_002735"
     tRNA            complement(1095..1170)
                     /locus_tag="O0V33_002735"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1135..1137),aa:Asp,seq:gtc)
     gene            complement(1180..1253)
                     /locus_tag="O0V33_002736"
     tRNA            complement(1180..1253)
                     /locus_tag="O0V33_002736"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1217..1219),aa:Met,seq:cat)
BASE COUNT          384 a          300 c          294 g          310 t
ORIGIN      
        1 tcattatcat taatgtaata ccaaaaaaag aagttaaaca atgtatagtt gcttaacttc
       61 ctatattaaa ctcatcatta caatttgaca tagagcctat taaagcgtgt gccatttgag
      121 tgccacttta tttgtattgt atagagagaa ataaaaagaa accttgtttt acaaggtttc
      181 tagtacgtta tgttatgtaa ataacagtta attataccgg tggtcggggt cgaaccgaca
      241 ctccacaagt ggaacgggat tttgagtccc gcgcgtctgc caattccgcc acaccggctt
      301 aatggtaaac aaaaaacttc cctttggaag caattatgga gcggaagata ggatttacac
      361 ctatacctca ttccaggaag gaatgtattc taagagttga aatactcccg cattattatt
      421 aaattatgga gcggaagata ggatttgcac ctatacctcg ttccgggaag gaacgtgttc
      481 taaaagttga actactcccg cataaacctg gaggcggcaa ccggatttga accggtgata
      541 aaggttttgc agacctctgc cttaccactt ggctatgccg ccaataactg ggctagctgg
      601 attcgaacca acgagtgacg gagtcaaagt ccgttgcctt accgcttggc tatagcccat
      661 taataataag ggcggctgaa ggggatcgaa ccctcgaatg tcggaaccac aatccgatgt
      721 gttaaccact tcaccacagc cgccatggca ggggcagtag gaatcgaacc cacaccaaag
      781 gttttggaga cctctattct accgttgaac tatgccccta ttaaaaataa taaatggagg
      841 ggggcagatt cgaactgccg aacccgaagg agcggattta cagtccgccg cgtttagcca
      901 cttcgctacc cctccataaa tggtgccggc cagaggactt gaacccccaa cctactgatt
      961 acaagtcagt tgctctacca attgagctag gccggctaag aaatggttca ggacagagtc
     1021 gaactgccga cacatggagc ttcaatccat tgctctacca actgagctac tgaaccataa
     1081 taaaaatgta atgatggcgg tctcgacggg aatcgaaccc gcgatctcct gcgtgacagg
     1141 caggcgtgtt aaccgctaca ctacgagacc tataaaatat tgcgggaggc ggatttgaac
     1201 caccgacctt cgggttatga gcccgacgag ctaccgaact gctccatccc gcgataataa
     1261 aaaataatgg cggaggaaga gggattcg
//
LOCUS       DAKSQE010000236         1278 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.239, whole genome shotgun sequence.
ACCESSION   DAKSQE010000236 DAKSQE010000000
VERSION     DAKSQE010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.239"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..1138
                     /gene="scpA"
                     /locus_tag="O0V33_002737"
     CDS             <1..1138
                     /gene="scpA"
                     /locus_tag="O0V33_002737"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HCX8977295.1"
                     /translation="LSLIFSLSITPIANAESNSNIKAKDKRHVQVNVEDKSVPTDVRN
                     LAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVLNTEGNIDYIV
                     TISPKVTKDSSSSSKYTINVSSFLSKALNEYKDQQITILTNSKGYYVVTQNHKAKLVL
                     KTPRLEDKKAKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQYVNKLENFKI
                     RETQGNNGWCAGYTMSALLNATYNTNKYHAEAVMRFLHPNLQGQQFQFTGLTPREMIY
                     FGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAILGSRVESRNGMHAGHAMAVVGNAKL
                     NNGQEVIIIWNPWDNGFMTQDAKNNVIPVSNGDHYQWYSSIYGY"
     gene            1169..>1278
                     /locus_tag="O0V33_002738"
     CDS             1169..>1278
                     /locus_tag="O0V33_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HCX8977296.1"
                     /translation="MEQFELFSIDKFKCNSEAKYYLNIIEGEWHPQDLND"
BASE COUNT          494 a          185 c          209 g          390 t
ORIGIN      
        1 tttaagttta atattttctt taagtattac ccctattgca aacgctgaga gcaattcaaa
       61 tatcaaagca aaagataaga ggcatgtgca agttaatgtc gaggacaaga gtgttccaac
      121 tgacgtacgt aatttagcgc aaaaagacta tctttcttat gttacttcac ttgataaaat
      181 ctataataaa gagaaagcct cgtatacact tggtgaacct tttaaaattt ataaattcaa
      241 caaaaagagt gatggtaatt attattttcc tgttttgaac actgaaggta atatcgatta
      301 tatagttaca atttcaccaa aagtcactaa ggattctagt tcatcttcta aatacacaat
      361 caatgtttct tcatttttat ctaaagcctt aaatgaatat aaagatcaac aaattactat
      421 tttgacaaac tcaaaaggtt actatgtggt tactcaaaat cataaagcta aactagtact
      481 aaaaacacca cgtctagaag ataaaaaggc aaaaaagacc gagagtatac ctactggcaa
      541 taatgtaacg caactaaagc aaaaagcttc tgtcactatg ccaactagtc aatttaaaag
      601 taataattat acttacaacg agcaatatgt aaataaactt gagaatttca agataagaga
      661 aactcaaggt aacaatggtt ggtgtgcagg ctatacgatg tctgcattac taaatgcgac
      721 ttataatact aacaaatatc atgcagaagc tgtgatgaga tttttacacc caaacttaca
      781 aggacaacaa ttccaattta ctggtttaac gccaagagag atgatttatt ttggacaaac
      841 tcagggaaga agtccacaac ttcttaatag aatgactaca tataatgaag ttgacaatct
      901 tactaaaaat aataaaggaa ttgctatttt aggtagtaga gtagaatcaa gaaatggtat
      961 gcacgcaggg catgctatgg cagttgttgg gaacgctaaa ttaaacaatg gacaagaggt
     1021 tataattata tggaatccat gggataatgg ttttatgact caagatgcta aaaacaatgt
     1081 tattccagta tccaatggcg accattatca atggtattca tctatttatg gttattaaaa
     1141 tttgtatttt agaaaggtaa ataatattat ggaacaattt gaattattta gtattgataa
     1201 attcaaatgt aattcagagg ctaagtatta tcttaatatt attgagggag aatggcatcc
     1261 tcaagattta aatgatag
//
LOCUS       DAKSQE010000237         1276 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.240, whole genome shotgun sequence.
ACCESSION   DAKSQE010000237 DAKSQE010000000
VERSION     DAKSQE010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.240"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            13..127
                     /gene="rrf"
                     /locus_tag="O0V33_002739"
     rRNA            13..127
                     /gene="rrf"
                     /locus_tag="O0V33_002739"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            139..214
                     /locus_tag="O0V33_002740"
     tRNA            139..214
                     /locus_tag="O0V33_002740"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:172..174,aa:Val,seq:tac)
     gene            231..306
                     /locus_tag="O0V33_002741"
     tRNA            231..306
                     /locus_tag="O0V33_002741"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:264..266,aa:Thr,seq:tgt)
     gene            312..387
                     /locus_tag="O0V33_002742"
     tRNA            312..387
                     /locus_tag="O0V33_002742"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:345..347,aa:Lys,seq:ttt)
     gene            391..472
                     /locus_tag="O0V33_002743"
     tRNA            391..472
                     /locus_tag="O0V33_002743"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:425..427,aa:Leu,seq:tag)
     gene            476..550
                     /locus_tag="O0V33_002744"
     tRNA            476..550
                     /locus_tag="O0V33_002744"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:508..510,aa:Gly,seq:gcc)
     gene            561..649
                     /locus_tag="O0V33_002745"
     tRNA            561..649
                     /locus_tag="O0V33_002745"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:595..597,aa:Leu,seq:taa)
     gene            668..741
                     /locus_tag="O0V33_002746"
     tRNA            668..741
                     /locus_tag="O0V33_002746"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:702..704,aa:Arg,seq:acg)
     gene            751..824
                     /locus_tag="O0V33_002747"
     tRNA            751..824
                     /locus_tag="O0V33_002747"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:785..787,aa:Pro,seq:tgg)
     gene            842..917
                     /locus_tag="O0V33_002748"
     tRNA            842..917
                     /locus_tag="O0V33_002748"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:875..877,aa:Ala,seq:tgc)
     gene            939..1012
                     /locus_tag="O0V33_002749"
     tRNA            939..1012
                     /locus_tag="O0V33_002749"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:973..975,aa:Met,seq:cat)
     gene            1041..1114
                     /locus_tag="O0V33_002750"
     tRNA            1041..1114
                     /locus_tag="O0V33_002750"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1075..1077,aa:Ile,seq:cat)
     gene            1124..1213
                     /locus_tag="O0V33_002751"
     tRNA            1124..1213
                     /locus_tag="O0V33_002751"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1160..1162,aa:Ser,seq:tga)
BASE COUNT          287 a          303 c          350 g          336 t
ORIGIN      
        1 tacattcata tgtctggtga ctatagcaag gaggtcacac ctgttcccat gccgaacaca
       61 gaagttaagc tccttagcgt cgatggtagt cgaacttacg ttccgctaga gtagaacgtt
      121 gccaggcagt tttaaatcgg agaattagct cagctgggag agcatctgcc ttacaagcag
      181 agggtcggcg gttcgaaccc gtcattctcc accatttatt cttacatatt gccggcctag
      241 ctcaattggt agagcaactg acttgtaatc agtaggttgg gggttcaagt cctctggccg
      301 gcaccatgga agagccatta gctcagttgg tagagcatct gacttttaat cagagggtca
      361 gaggttcgaa tcctctatgg ctcaccattt gcgggtgtgg cggaattggc agacgcacta
      421 gacttaggat ctagcgcctt acggcgtggg ggttcgactc ccttcacccg catatgcaga
      481 agtagttcag cggtagaata caaccttgcc aaggttgggg tcgcgggttc gaatcccgtc
      541 ttctgctcca tttttatagt gccggggtgg cggaactggc agacgcacag gacttaaaat
      601 cctgcggtga gtgatcaccg taccggttcg attccggtcc tcggcaccat tttcaataaa
      661 aatatatgcg cccgtagctc aattggatag agcgtttgac tacggatcaa gaggttatgg
      721 gttcgactcc tatcgggcgc gttaattata cgggaagtag ctcagcttgg tagagcactt
      781 ggtttgggac caaggggtcg caggttcgaa tcctgtcttc ccgatatact gtaattatta
      841 tgggggctta gctcagctgg gagagcgcct gctttgcacg caggaggtca gcggttcgat
      901 cccgctagtc tccaccatat tatttacaaa ctatataagg cggtgtagct cagctggcta
      961 gagcgtacgg ttcatacccg tgaggtcggg ggttcgatcc cctccaccgc cactatttat
     1021 tagttgtaaa attatattta ggacctttag ctcagttggt tagagctaac ggctcataac
     1081 cgttcggtcg caggttcgag tcctgcaagg tccatataat tttggaggaa tacccaagtc
     1141 cggctgaagg gatcggtctt gaaaaccgac aggggcttaa cggctcgcgg gggttcgaat
     1201 ccctcttcct ccgttttact aatggtctcg tagtgtagcg gttaacacgc ctgcctgtca
     1261 cgcaggagat cgcggg
//
LOCUS       DAKSQE010000238         1244 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.241, whole genome shotgun sequence.
ACCESSION   DAKSQE010000238 DAKSQE010000000
VERSION     DAKSQE010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.241"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            97..1207
                     /gene="prfB"
                     /locus_tag="O0V33_002752"
     CDS             join(97..168,170..1207)
                     /gene="prfB"
                     /locus_tag="O0V33_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCX8977297.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDGEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
BASE COUNT          481 a          166 c          254 g          343 t
ORIGIN      
        1 cttaacattg ttatttaaca aaattatgtt aaaatttagc attataaaag atataaatca
       61 atgacttgaa ttgaattata aataggagcg aaggctatgg aattatcaga aatcaaacga
      121 aatatagata agtataatca agatttaaca caaattaggg ggtctctttg acttagagaa
      181 caaagaaact aatattcaag aatatgaaga aatgatggca gaacctaatt tttgggataa
      241 ccaaacaaaa gcgcaagata ttatagataa aaataatgcg ttaaaagcaa tagttaatgg
      301 ttataaaaca ctacaagcag aagtagatga catggatgct acttgggatt tattacaaga
      361 agaatttgat ggagaaatga aagaagactt agagcaagag gtcattaatt ttaaggctaa
      421 agtggatgaa tacgaattgc aattattatt agatggacct cacgatgcca ataacgcaat
      481 tctagagtta catcctggtg caggtggcac ggaatctcaa gattgggcta atatgctatt
      541 tagaatgtat caacgttatt gtgagaagaa aggttttaaa gttgaaactg ttgattattt
      601 acctggagat gaagcaggga ttaaaagtgt aacattgctc atcaaagggc ataatgctta
      661 tggttattta aaagctgaaa aaggtgtaca ccgactagta cgaatttctc catttgattc
      721 atcaggacgt cgtcatacat catttgcatc atgtgacgtg attccagatt ttaataatga
      781 tgaaatagag attgaaatca atccggatga tattacagtt gatacattca gagcttctgg
      841 tgcaggtggt cagcatatta acaaaactga atcggcaata cgaattaccc atcacccctc
      901 aggtatagtt gttaataacc aaaatgaacg ttctcaaatt aaaaaccgtg aagcagctat
      961 gaaaatgtta aagtctaaat tatatcaatt aaaattggaa gagcaggcac gtgaaatggc
     1021 tgaaattcgt ggcgaacaaa aagaaatcgg atggggaagc caaattagat catatgtttt
     1081 ccatccatac tcaatggtga aagatcatcg tacgaacgaa gaaacaggta aggttgatgc
     1141 agtgatggat ggagacattg gaccatttat cgaatcatat ttaagacaga caatgtcgca
     1201 cgattaatat atattttaag ggagtgggac agaaatgata aaga
//
LOCUS       DAKSQE010000239         1240 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.242, whole genome shotgun sequence.
ACCESSION   DAKSQE010000239 DAKSQE010000000
VERSION     DAKSQE010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.242"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..415)
                     /locus_tag="O0V33_002753"
     CDS             complement(<1..415)
                     /locus_tag="O0V33_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCX8977298.1"
                     /translation="MTAIDKVYYEDVLEDDILNLLKPYIQGKEKELYITSDNFAIDLN
                     KVTALLDLKIQYEDLDDDVSGYIDERTIYVNKNHSSNRKRFTIAHEIGHFLHNHKNKI
                     KYRNNSYAYDTEEMIEERVANDFSANLLMPKKQMEA"
     gene            complement(608..1054)
                     /locus_tag="O0V33_002754"
                     /pseudo
     CDS             complement(608..1054)
                     /locus_tag="O0V33_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
BASE COUNT          387 a          211 c          126 g          516 t
ORIGIN      
        1 aagcttccat ttgctttttg ggcattagta aattagctga aaagtcatta gccacccttt
       61 cttcaatcat ttcttcagtg tcatatgcat aactattatt tctatatttg attttatttt
      121 tatgattgtg taaaaaatgt cctatttcat gggcaattgt aaatcttttt ctattactag
      181 aatgattttt atttacataa atagttcttt catcaatata acccgaaaca tcatcatcca
      241 aatcttcata ctgaattttt aaatccagta atgcggtcac tttatttaaa tcaattgcaa
      301 aattatcaga agtgatataa agttcctttt ctttcccctg tatatatggt ttaagcagat
      361 taagtatgtc atcttctaat acatcttcat aataaacttt gtcaatggca gtcataaaaa
      421 tcgcctccta aaagatattg ttaatcttct ctatctatta attatatttt atattctaaa
      481 gcttaaaata accagtctaa ctttcattat tttagtcact aattaaatat gcgcatatct
      541 ttacttacat ttattgtttt aatcactaaa attaataata aaatattttt tcactgtaca
      601 accgaatcta atccaacgat cccgaaaaga agaacaacgg tggctcggct tcatattcat
      661 ttttgtctaa gtgttatttt tcaattattt catccactgt taatcggtct ttagcatctt
      721 tctctttcaa taatcgccct attttttctt ccttatatcc tatgctacta ttaaactgat
      781 ttccttcatc tcctgtattt atatatgcct gaaatttata ttgtttatca ttatttatat
      841 atcctttaat aaagtaagca ttcacagggc ttttttgaaa ctttgttatt ttcatagact
      901 tatagtgatt caggttatgc ttaaaatata aatcgattcg cttttcttgt gtctcaatat
      961 aatgttcttt tttacttttg tgaaacgcgt atgcaataac aattagtact attaataatg
     1021 caacaactac cacaaataca tatctctttt tcattttaac ccccatagtt tagtatttat
     1081 ttcttataga ttttagattt caaaaaacaa ttcactttga catgctatct tttttctatg
     1141 ttccgtttac gcccctcatg aaacgttgtt tcaaaatact tttaaaaatt atttgcttga
     1201 cataattata aatacataaa ataacgctgt tatatatggg
//
LOCUS       DAKSQE010000240         1216 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.243, whole genome shotgun sequence.
ACCESSION   DAKSQE010000240 DAKSQE010000000
VERSION     DAKSQE010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.243"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(30..1031)
                     /locus_tag="O0V33_002755"
     CDS             complement(30..1031)
                     /locus_tag="O0V33_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HCX8977299.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDIVQIIYEAILKLQNPNKKDDMTILIIKRVN"
BASE COUNT          380 a          223 c          153 g          460 t
ORIGIN      
        1 cgaaataatt tctaatcttc ttttttaaat taatttactc tttttataat caaaatagtc
       61 atatcatctt ttttatttgg gttttgaagt tttaaaattg cttcatagat aatttgaaca
      121 atatcttgtg ggtgcatatg tttatgtttt ttaatatatt ctaaaagttt ttgtttatct
      181 ataaaggtac cttcactatt tctagcttca gtcacaccat ccgttaaaat gataattaaa
      241 tcatcaaggt atattggaat ttcttgttgt tgatatcgtg tttgtgaact gattcctaac
      301 actctacctc taactgaaat ttcttcaaac tcttcttttt cagcgcgata aatatatcca
      361 ggctcatgac cagctgaact acaatataat aaatggttca tttcttcata taaaccataa
      421 aacattgtga cgaacatatt ttgattaata ttcttttcaa caacacgatt taaacgtttt
      481 aagccatcac tcggtaattg tgagtgtcca taagaatcca tgccaaactt tatcatactc
      541 attgctaaag cagctggtat accttttcca atgacatctg caacagcaaa gctcattgtg
      601 ccatcgttat ggtcaattaa attaaaataa tctccactta ctttttgtgc cgccactgaa
      661 ataacgccaa tttgaatact atcaaattgt ggaatatctg ttttaagcat tgtttgttgt
      721 aagctagaag ctaagtctat ctctttatcg tgaacttgaa gtttatctac caatctttga
      781 taatctcgat aactataacc aaagccttta acgatttctt gtaagacatc taatgtcttg
      841 aacacatctt cacgcgttaa gtttaacgtc aatatatagt ttttgtgaat atcgacaatg
      901 tcttcaggca agacgtcctt acgaatcact tcgtcagtgt atttctcaca cttttttatc
      961 aattctactt tatcttggca cgttaaactt tcatcaatta aacccttata atgttgctta
     1021 aattcttcca cgattagttg cctcctcact tcccttacaa agcaaatgct taagcaagga
     1081 taattatgat tcacttacta acatttattt aattgcactt atcaattttg tcgtttgcaa
     1141 ttttgctgta gatgatactt ttaagctaaa aaaactttag gatggaatac ctaaagccta
     1201 aaaaacttaa gacatc
//
LOCUS       DAKSQE010000241         1194 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.245, whole genome shotgun sequence.
ACCESSION   DAKSQE010000241 DAKSQE010000000
VERSION     DAKSQE010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.245"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(1..488)
                     /locus_tag="O0V33_002756"
                     /pseudo
     CDS             complement(1..488)
                     /locus_tag="O0V33_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
     gene            complement(509..745)
                     /locus_tag="O0V33_002757"
     CDS             complement(509..745)
                     /locus_tag="O0V33_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977300.1"
                     /translation="MIRFDVNGSDHANSPNNERIPTPHIHIYTEEYNNGGIAIPLKDI
                     EDLELTDEIIESLDFFMKYTNIKHDNVIIEPRLL"
     gene            complement(846..1074)
                     /locus_tag="O0V33_002758"
                     /pseudo
     CDS             complement(846..1074)
                     /locus_tag="O0V33_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121211.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          381 a          169 c          168 g          476 t
ORIGIN      
        1 aaacctgatt ctccagaaac agaaacttgt gctaaacctc ttatttctcg gtcatataag
       61 aattcaaata cttcttcata gaatctgttt gtaacgttag atttagtagt taaagtaagg
      121 tcataaattt tcaatatgcc ttgaattaaa ttatgtttag actgagcaaa actctaattt
      181 ttaacatccg ctgtaatttc tttatcaact aattttaaat taaattgatt taaaatattc
      241 tgtataagtt tagttctcgt ttttgtgttt atatcaatac ccaacatttc taattcatta
      301 atcgtcaatc cgttatctga tagacaaatt tcattattag gtagtgtatc agcatatatt
      361 ctaataaaat cattcaaatg gttcttaaag ggagtagtaa tttctgtaga actatctaat
      421 tctttgaaga tataattttg ttttaaccag ttgaaatatt cgttcattct ttcttcgata
      481 gtcttcaata agtatcccgc catttctttt acagtaatct tggttctatt ataacattat
      541 cgtgttttat attagtatac ttcataaaga agtctaatga ttctataatt tcatctgtta
      601 attctaaatc ttcaatatct tttaatggga tcgcaatacc accattatta tattcttcag
      661 tatatatgtg tatatgtggc gtaggtattc tttcattatt tggtgaattt gcatgatcgc
      721 taccattaac atcgaaccta atcatatcag tggttaaaac ataaatcctt ggttataaaa
      781 gaaaatcggt tttaaaaaat cgaatcacga ttaattcaga agagtagtgg tatttttaaa
      841 ttttacttaa tgatattgct aagttctgga gtaaatccag taggatgacc acaaagtttt
      901 cttgtagtta cataattctt ttgagccgtt tcagattgat ataacttatt tacattattt
      961 ctgctaattt cacgtaagat agtagatgcg gatcggtcta atttacgtgc aataggtctt
     1021 aaagaataat tttctctata cgtgctcagt tagtataaga tagttatcgc tcatattggc
     1081 aatacttaaa tgtgttttcg tggttgtaaa caccttacaa taaagtgccc attgtgggta
     1141 tttttatgaa tttttattgg gttgcactta atattacaat gtattgatta ataa
//
LOCUS       DAKSQE010000242         1187 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.246, whole genome shotgun sequence.
ACCESSION   DAKSQE010000242 DAKSQE010000000
VERSION     DAKSQE010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.246"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..>1187
                     /locus_tag="O0V33_002759"
     CDS             <1..>1187
                     /locus_tag="O0V33_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCX8977301.1"
                     /translation="ILKEDMLNEYYYLPKTLYNTLASPEFEDLKRTDASQVDGQDDTS
                     EDDDNESEKADSKSADSESKGGAYLEMELHEGQNSETLGNDEAREGDATDDMTDMMTK
                     KGKGSNDTLNREEGDAVGQSQAFQLDGVNKNVEIKWQIPEIEPQYVLEYQESKQDVQY
                     EIKDLIQIIKKTIEREQRDARFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKSFDAT
                     FTLLIDASASMHDKMAETKKGVVLFHETLKALNIKHEILSFSEDAFDSDDHVQPNIIN
                     EIINYDYSTFEKDGPRIMALEPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPS
                     AFNYSQDGIIDTYEAVEMSRKFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGV
                     AHLP"
BASE COUNT          450 a          166 c          235 g          336 t
ORIGIN      
        1 attttaaaag aagatatgct aaacgaatat tattacttac ctaaaacact atataataca
       61 ttagctagtc cagagtttga agatttaaag agaactgatg caagtcaagt cgatgggcaa
      121 gatgatactt cagaagatga tgataacgaa agtgaaaaag ctgactctaa atctgcagat
      181 agtgagtcaa aaggtggcgc ctatttagaa atggagcttc acgaaggaca aaatagtgaa
      241 acattaggta atgatgaagc acgtgaaggc gatgctacag acgatatgac tgatatgatg
      301 accaaaaagg gcaaaggatc aaacgatacg ctaaatcgtg aagaaggcga tgctgttggt
      361 caaagccaag catttcaact tgatggtgtg aacaaaaatg tagaaataaa atggcaaatt
      421 cctgaaattg agccacaata tgttttagaa tatcaagaat cgaaacaaga cgtacaatat
      481 gaaatcaaag acttaattca aattataaag aaaacgattg aacgagaaca acgagatgca
      541 cgttttaatt taacaaaagg gcgattacaa aaggatttaa ttaattggtt tatcgacgat
      601 cagtataaat tgttttacaa aaaacaagat ttaagtaaat catttgatgc gacgtttaca
      661 ttgttaattg atgcgtcagc aagtatgcat gataaaatgg ctgaaacgaa gaaaggtgta
      721 gtgttattcc acgagacgtt aaaagcattg aacattaaac atgaaatttt atcattcagt
      781 gaggatgcct ttgattcaga cgatcatgta caaccaaata tcattaatga aattattaac
      841 tatgattact caacctttga aaaagatggc ccacgtatta tggcacttga acctcaagat
      901 gataatcgtg atggtgttgc tattagagtt gcaagtgaac gattaatgcg tcgaaatcaa
      961 catcaacgat ttttaatcgt attttctgac ggcgaaccgt ctgcatttaa ttatagtcaa
     1021 gatggtatta ttgatacgta tgaagctgta gaaatgtcac gtaaatttgg cattgaagta
     1081 tttaatgtat ttttgagtca agatccaata actgaagatg ttgaacaaac aattcataat
     1141 atttatggtc aatatgccat tttcgttgaa ggtgttgcgc atttacc
//
LOCUS       DAKSQE010000243         1180 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.247, whole genome shotgun sequence.
ACCESSION   DAKSQE010000243 DAKSQE010000000
VERSION     DAKSQE010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.247"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            38..1114
                     /locus_tag="O0V33_002760"
     CDS             38..1114
                     /locus_tag="O0V33_002760"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCX8977302.1"
                     /translation="MSQAVKIELRETLKEKPDTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKKDGEVALFRPDENFKRLNNSLARLE
                     MPQVNEGELLEGLKQLVDLEREWVPEGEGQSLYIRPFVFATEGVLGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
BASE COUNT          412 a          159 c          247 g          362 t
ORIGIN      
        1 acaaattctt atactatatt attaggaggc aatcaccatg tcacaagcag ttaaaattga
       61 attacgcgaa acattaaaag aaaaaccaga tacatctcaa ttaggatttg gtaagtattt
      121 tacagattat atgttgagtt atgattatga tgcggataaa ggatggcatg atttgaagat
      181 tgtaccttat ggtccaattg aaatttcgcc agcagcacaa ggtgttcact atggacaatc
      241 agtgttcgaa ggtttaaaag catataaaaa agatggagag gttgcacttt tccgtccaga
      301 tgaaaacttt aagcgtctta ataactcgtt agcacgctta gaaatgcctc aagtcaatga
      361 aggtgaattg ctagaaggac ttaagcaatt agttgattta gagagagaat gggttccgga
      421 aggtgaaggt caatcattat atattcgtcc ttttgttttt gcgacagaag gggtacttgg
      481 cgttggtgca tcacatcagt ataaattatt aattatttta tctccttcag gtgcatatta
      541 tggtggtgaa actttaaaac caactaaaat ctatgtagaa gatgaatatg tacgtgctgt
      601 tcgtggcggt gtaggctttg cgaaagttgc aggtaactat gcggcaagtt tattagcaca
      661 aactaatgca aataaattag gttatgacca agtattatgg cttgatggtg ttgaacagaa
      721 atatatcgaa gaagttggta gcatgaacat tttcttcgtt gaaaatggca aagtaattac
      781 accagagttg aatggcagta ttctacctgg tattacacgt aaatctatta tcgaattagc
      841 taaaaactta ggatatgaag tcgaagagcg ccgcgtttca atcgatgaat tattcgaatc
      901 atatgataaa ggtgagttaa cagaagtatt tggtagtggt actgcagcag ttatttcacc
      961 tgtaggtaca ttgagatatg aagatcgtga aatcgttatt aataataatg agactggtga
     1021 aattactcaa aaattatacg acgtctatac tggtattcaa aatggtactt tagaagataa
     1081 aaatggttgg agagtcgttg taccaaaata ttaataaaaa ttgaatatga tcatgaaaac
     1141 aaaatgtaaa agttaatcat gactaaaatc cctttcaatg
//
LOCUS       DAKSQE010000244         1161 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.248, whole genome shotgun sequence.
ACCESSION   DAKSQE010000244 DAKSQE010000000
VERSION     DAKSQE010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.248"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            171..1160
                     /gene="trpS"
                     /locus_tag="O0V33_002761"
     CDS             171..1160
                     /gene="trpS"
                     /locus_tag="O0V33_002761"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCX8977303.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKASFKTVKKMEKAMGLGRKR"
BASE COUNT          436 a          176 c          201 g          348 t
ORIGIN      
        1 attaaatgag aaaaacctaa tttttcagat aagtttctac acttataaaa aagattatta
       61 atccctttgt tagtagcaag ttatacatat attctaacac atcttacatt tttaagaaat
      121 actgttataa tgataattgt taaattatta ctaagaaagt aggcatttaa atggaaacat
      181 tattttcagg cattcaacct agtggaattc ctactattgg aaattatatt ggcgcattaa
      241 agcaatttgt tgatgtgcaa aacgactatg attgttattt ctgtatcgta gatcaacatg
      301 caattacaat gccacaagat cgcttaaaat tacgtaaaca aactagacaa ttagcagcga
      361 tttatttagc ttctggtata gatccagaca aagcaacatt gttcatacaa tctgaagtcc
      421 ctgcacacgt acaagcagga tggatgttaa ctacaattgc ttctgttggc gaattagagc
      481 gtatgacgca atacaaagat aaagctcaga aagcagttga aggtatacca gctggtctat
      541 taacatatcc acctttaatg gcagctgata ttgttcttta caatactaat atcgttccag
      601 ttggcgatga ccaaaagcag cacattgaat taacacgtaa tcttgtagat agatttaata
      661 gtcgttataa tgatgtcctt gtgaaacctg aaattcgtat gcctaaagtc ggtggccgtg
      721 ttatgagttt acaagatcca acaagaaaaa tgagtaaaag tgatgacaac gctaaaaact
      781 ttatttcatt attagacgaa ccaaatgttg ctgctaaaaa aattaaaagt gctgtgactg
      841 attcagacgg tattattaaa ttcgaccgcg acaataaacc aggtattaca aatttaattt
      901 caatatacgc tggattaaca gacatgccta ttaaagatat tgaggcaaaa tatgaaggcg
      961 aaggttacgg taaattcaaa ggcgaccttg ctgaaatagt taaagcattt ttagtagaat
     1021 tccaagaaaa atacgaaagt ttctataact cagataaact tgatgatatt ttagatcaag
     1081 gtagagataa agcacataaa gcttcattta aaactgtcaa aaaaatggaa aaagcgatgg
     1141 gtttaggacg taagagataa a
//
LOCUS       DAKSQE010000245         1135 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.249, whole genome shotgun sequence.
ACCESSION   DAKSQE010000245 DAKSQE010000000
VERSION     DAKSQE010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.249"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            68..301
                     /locus_tag="O0V33_002762"
     CDS             68..301
                     /locus_tag="O0V33_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977304.1"
                     /translation="MVFKDWEINVVYSGKHEVVTNENSLFVIDEDYDVAIAINYLDNK
                     LKVSHVNYGSEFTIDASNKVLALMIHNPNIDEN"
     gene            911..1096
                     /locus_tag="O0V33_002763"
     CDS             911..1096
                     /locus_tag="O0V33_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977305.1"
                     /translation="MNKHINKKTTKHLEDYKKVQQRNSEETNRGCLKSAAMLIVLFLF
                     IIFGIVACSTHVNFFFQ"
BASE COUNT          429 a          156 c          159 g          391 t
ORIGIN      
        1 tgtaagaagt tgtatgaata agttaaactt agattgaatc agtttaaatg ttcaaggagg
       61 atgacaaatg gtttttaaag attgggaaat taatgttgtt tactcaggga aacatgaagt
      121 agttacaaac gaaaatagtt tatttgttat tgatgaagat tatgatgtcg caatcgcaat
      181 caattattta gataataaat taaaagttag tcatgttaac tatggctctg aatttacaat
      241 tgacgcatct aacaaagtac ttgcgttgat gattcataat ccaaatattg acgagaatta
      301 aatattaaaa aacatttaga aacataaagg catactatat atagtctttt aaacgtataa
      361 cgaacgaaaa ttagaaattc ggatattcat tacactatta aaatgcaatt tttcaaagca
      421 tttaaaaaca acagttaagg tggtgaccaa aaattaacat tgtgtataac atcattgtat
      481 atatagcatg catgattaac ttcggactga ttatcgtccc attgctaact accaatgacg
      541 cagattttgt ttggacttct tcgtcattaa tatatatgat ccttttaaca ctacttatat
      601 taattgttta tattaaaact aaagatagtt tacagctaac aatatttata cttagtgtgg
      661 cattaatcct tctatactgt gcacctttac tgttcattta tgtattctaa tgtttcatta
      721 atattttaat acgtcagttt aaaaacaaac aaattggaaa ataaacgaaa caagttatca
      781 tctaatttaa catgatactc acttaaaatt tgaaacaacg agcagcctaa cttaagcttt
      841 atatctttat ttcaagttat gttttcaact attttaatgc aatttacatt tagattaagg
      901 agtaaacatc atgaataaac atataaataa aaagaccaca aagcatcttg aagattataa
      961 aaaggttcaa caaagaaatt cggaggaaac aaatagaggt tgtttaaaaa gcgccgccat
     1021 gcttattgtc ttatttctat tcattatatt tggcatcgtg gcatgttcga cacacgttaa
     1081 ctttttcttc caataatata ctcattgagt aaaaatcaga acgtttcata ctacg
//
LOCUS       DAKSQE010000246         1124 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.250, whole genome shotgun sequence.
ACCESSION   DAKSQE010000246 DAKSQE010000000
VERSION     DAKSQE010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.250"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            57..809
                     /locus_tag="O0V33_002764"
                     /pseudo
     CDS             57..809
                     /locus_tag="O0V33_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
BASE COUNT          449 a          120 c          188 g          367 t
ORIGIN      
        1 tttcctagcc tgtttatcat ttatgtctcg ctctcaaatg taaattaata attgttttgg
       61 gggagtttga agtaaaatat ttaacaggat ttattttaat attattgtta gaaggaattt
      121 ttacaaattc agcaagtgca atcgaatatt cagacttaca tcataaaagt aaacttgatt
      181 ccaagcgtct atataatgct aaggtgtcat ttgctaatcc aactgatctt gaaaataaaa
      241 aaacaaatga tagactgttg aagcatgatt tgttatttca tgacatgttc ttaaatgatg
      301 cttggaaaaa agatttaaaa gttgaattcg aaaatgaggc actttcaaag aaatttataa
      361 ataagaatat tgatgtatat gctggaagct atagctatga atgtcatggg ggagaaacca
      421 ataaaacgca atgtagttat ggtggtgtta ctttaagtga caataataaa tacgatgatt
      481 ataagaatat accttgtaat ttatggattg acggacatca aacagaaata gaactaactg
      541 cagtgaaaac gaaaaagtaa attgttacca ttcaagaatt agatgttcaa ttaagaaatt
      601 atttgaatga gaagtataag ttgtacgaac aaggtggcga cattgttaaa gggtatgtta
      661 aatatcataa tgatgatgaa aaaaatgtag aatatgattt ttataattta aatggtgagt
      721 atggtcatga ggtattaaaa atgtatgctg ataataaaac tatcaatagt gacaaattgc
      781 atttagatat ctatttattc aaatcataat ttaagtgagt taatcattaa tgatctttga
      841 cagtgaaata taaagtacct gtcattatat tgttacaaat aaaaacagca gtaaggaggc
      901 tattactcag aaaagtctta ctgctgtttt ctattagaat aatctttaat gcacacagga
      961 attcgatacg aaacaaatat gattaactgt ataattacaa aataaaacta ctatagtaaa
     1021 aagaagataa agacgatgat aataaatgca tcgaatataa ttttcttaat gtttttatca
     1081 acaacatcag gtaaggacat aagtataaat cctagaatta gagt
//
LOCUS       DAKSQE010000247         1105 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.251, whole genome shotgun sequence.
ACCESSION   DAKSQE010000247 DAKSQE010000000
VERSION     DAKSQE010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.251"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(315..>1105)
                     /locus_tag="O0V33_002765"
     CDS             complement(315..>1105)
                     /locus_tag="O0V33_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977306.1"
                     /translation="SYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAIWNF
                     LFLRRALPLTKQELGEEEPELSRTSKGNVTNQTKIHLKQLQDKTTEYARKTRRSVDLD
                     KIRAKRDKFKKKVNDIIDIQEDDIPDWMRKPKWVKPMYVELFCGVVIFLFTFLEFNNR
                     NALFVSGDWKLSQTQYVIEWVTLLILLFIIIAYIATTLTFHLKGKFYYLQLFMGSILF
                     FKLLTEFINIMIHGLLLSVFITPTLLLMLLAIIISYSLQLRERP"
BASE COUNT          388 a          184 c          136 g          397 t
ORIGIN      
        1 ttcgaatgat atatgttaaa cttctataaa tgagatacta tatatcgttt cgcaaattcc
       61 acttgtcact tcaactattt actagttttc ttaattttac aaatggataa cttttcaact
      121 attttatttg cactaaaact ttgtatacaa ttttcaatac ttaattcaca ttacctgaat
      181 caatcaaatc tagttacaaa aaaagtacta tccataaact tcattgacct gtatctaagt
      241 aagttagaga tatatctaac ttatagcgta cacatcaaaa tgtttaatag atagtacttt
      301 tataatgctt ttaattatgg tcgctctcgt aattgtaacg aataagaaat gatgattgcc
      361 aataacatta atagtaatgt tggcgtaatg aacactgaaa gtagaagtcc atgaatcatt
      421 atatttataa attccgttaa caatttaaag aataaaatgc tccccataaa taattgtaaa
      481 taataaaact tacctttcaa gtggaaagtt aacgttgtag cgatatatgc gataataatg
      541 aatagcagaa ttagtaatgt aacccattca ataacatatt gtgtctgtga taatttccaa
      601 tcaccagata caaataatgc attacgatta ttaaattcta aaaatgtgaa taaaaagatg
      661 acgacaccac aaaatagttc gacatacatc ggtttaaccc atttcggttt tctcatccaa
      721 tcaggaatat cgtcctcttg aatatcgata atatcattaa ctttcttttt gaatttatct
      781 cttttagctc taattttatc taaatcgaca cttcttcttg tcttacgtgc atattctgta
      841 gtcttatctt ggagttgctt caagtgaatt ttagtttgat tcgtgacatt ccctttactt
      901 gttcttgata attctggctc ctcttcacct aattcttgct ttgttaaagg taaagctcgg
      961 cgcaagaaca agaaattcca aatagccatt tgaccaagta accacataat tataaagaaa
     1021 gcattgacac ttaagatatc aattgattct atttcattag catcaattct gccatacaat
     1081 gcaatttgtg taattaaata actta
//
LOCUS       DAKSQE010000248         1075 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.253, whole genome shotgun sequence.
ACCESSION   DAKSQE010000248 DAKSQE010000000
VERSION     DAKSQE010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.253"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..389
                     /locus_tag="O0V33_002766"
     CDS             <1..389
                     /locus_tag="O0V33_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977307.1"
                     /translation="MGYLSSRYLTKYLNYKFVSEICLVIFLIIYTYQSFIAVTISMIF
                     LGISSGLTRPQTINKLSSSSNLRVMLNYAETLYFIFNIAFLLMGGYLYTIGTIQYLIL
                     FIALLIFIYLIIIFYFTRREQHETKN"
     gene            373..888
                     /locus_tag="O0V33_002767"
     CDS             373..888
                     /locus_tag="O0V33_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HCX8977308.1"
                     /translation="MKLKTEFKGNNIPYEYAAGADVSDSINGNPIKSFPFEVTELPEG
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSVSGNSISIKADLSRTKGDYVQGKNSFTS
                     GLLAEDFSEIENHYVGPTPPDKDHQYELTVYALDHSLNLKNGFYLNEFLKEVNQHKID
                     QTSINLIGRKI"
BASE COUNT          400 a          124 c          166 g          385 t
ORIGIN      
        1 ttatgggtta tttatcttca cgttatttaa ccaaatactt gaactataag tttgtgtcag
       61 aaatttgttt agtaatattt ttaataatat atacatatca aagtttcata gcagttacta
      121 tttctatgat atttttaggt atttcttcag ggttaactcg tccacaaact ataaataaac
      181 tttctagcag tagtaactta agagtgatgc ttaattatgc agaaacgtta tattttattt
      241 ttaatatcgc atttttactt atgggtggtt acttatatac aataggaact attcaatact
      301 taatattatt tattgcatta ttaattttta tatatttaat aataatattt tattttacaa
      361 ggagagagca acatgaaact aaaaactgaa tttaaaggga acaatatacc atatgaatac
      421 gcagcaggtg cagatgtgag tgattctatt aacgggaatc caattaaatc atttccattt
      481 gaagtaactg aattaccgga agggactaaa tatcttgctt ggtctttaat tgactatgat
      541 gcaatcccag tatgtggctt tgcttggatt cattggagtg tagctaatgt aagtgttagt
      601 ggcaattcaa tttctattaa agcagattta tcaagaacaa agggcgacta tgtacaaggt
      661 aaaaatagct tcactagtgg tttgttagct gaagattttt cagaaataga aaatcactat
      721 gtaggaccaa caccacctga taaagatcat caatatgaat taacagttta tgcgttagat
      781 cattctttaa atttgaagaa tgggttctac ttgaatgaat ttttaaaaga agtaaatcaa
      841 cataaaatag atcaaacaag tattaacctt ataggaagaa aaatttaata ttaaatatct
      901 catcaatata aaattgttca attaaaagta cgaagaaaca agggatttaa tttatatatt
      961 aggtacggcg ttcgctataa tgcaatgaag taattaaatt taagaaatgt aaacttagtt
     1021 attgtaatgt gaatttattt gaaaaaatag aaagtattaa caattatagc tttta
//
LOCUS       DAKSQE010000249         1047 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.254, whole genome shotgun sequence.
ACCESSION   DAKSQE010000249 DAKSQE010000000
VERSION     DAKSQE010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.254"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..>1047
                     /locus_tag="O0V33_002768"
     CDS             <1..>1047
                     /locus_tag="O0V33_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCX8977309.1"
                     /translation="RHRVIIRDSNKQWREFIINWVQDTMDGYTEIECIASYLADITTA
                     KPYAPGKFEKKTTSEALKDVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTT
                     YKMVLDFYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALI
                     AVGPENDKGKRLELVVTDDEAQSQFNLPMRYIWGIYEPQSDDQNMNETRLSSLAKTEL
                     NKRKSAVMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIIS
                     ENSTYTFGQPKEFKESELREEFNKRLNLIHQKLNDNISNINTIVKDIVDGELEYFERK
                     IHKSDTPPENPVND"
BASE COUNT          408 a          156 c          203 g          280 t
ORIGIN      
        1 aacgacatcg tgttattata agggattcaa acaaacaatg gcgtgaattt attattaact
       61 gggttcaaga tacgatggac ggctacacag agatagaatg tatagcgtct tatcttgctg
      121 atataacaac agctaaaccg tatgcaccag gaaaatttga gaaaaagaca acttcagaag
      181 cattgaaaga tgtgttgagc gatacaggtt gggaagtttc tgaacaaacc gaatacgatg
      241 gcttacgtac tacgtcatgg acttcttatc aaactagata tgaagtttta aagcaattat
      301 gtacaaccta taaaatggtt ttagattttt atattgagct tagctctaat accgtcaaag
      361 gtagatatgt agtactcaaa aagaaaaaca gcttattcaa aggtaaagaa attgaatatg
      421 gtaaagattt agtcgggtta actaggaaga ttgatatgtc agaaatcaaa acagcattaa
      481 ttgctgtggg acctgaaaat gacaaaggga agcgtttaga gctagttgtg acagatgacg
      541 aagcgcaaag tcaattcaac ctacctatgc gctatatttg ggggatatat gaaccacaat
      601 cagatgatca aaatatgaat gaaacacgat taagttcttt agccaaaaca gagttaaata
      661 aacgtaagtc ggcagttatg tcatatgaga ttacttctac tgatttggaa gttacgtatc
      721 cgcacgagat tatatcaatt ggcgatacag tcagagtaaa acatagagat tttaacccgc
      781 cattgtatgt agaggcagaa gttattgctg aagaatataa cataatttca gaaaatagca
      841 catatacatt cggtcaacct aaagagttca aagaatcaga attacgagaa gagtttaaca
      901 agcgattaaa cctaatacac caaaaattaa acgacaatat tagcaatatc aatactatag
      961 taaaagatat tgtagatggt gaattagaat actttgaacg caaaattcat aaaagtgata
     1021 caccgccaga aaatccagtc aatgata
//
LOCUS       DAKSQE010000250         1047 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.255, whole genome shotgun sequence.
ACCESSION   DAKSQE010000250 DAKSQE010000000
VERSION     DAKSQE010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.255"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..>1047
                     /locus_tag="O0V33_002769"
     CDS             <1..>1047
                     /locus_tag="O0V33_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCX8977310.1"
                     /translation="RHRVIIRDSNKQWREFIINWVQDTMDGYTEIECVASYLADITTA
                     KPYAPGKLEKKTTSEALKEVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTT
                     YKMALDFYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALI
                     AVGPENDKGKRLELVVTDDEAQSQFNLPTRYIWGIYEPQSDDQNMNETRLRSLAKTEL
                     NKRKSAVMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIIS
                     ENSTYTFGQPKEFKESELREEFNKRLNIIHQKLNDNISNINTIVKDVVDGELEYFEHK
                     IHKSDTPPENPVND"
BASE COUNT          403 a          160 c          209 g          275 t
ORIGIN      
        1 aacgacatcg tgttattata agggattcaa acaaacagtg gcgtgaattt attattaact
       61 gggttcaaga tacgatggac ggctacacag agatagagtg tgtagcgtca tatctcgctg
      121 atataacaac agctaagcca tacgcaccag gtaaacttga gaaaaagaca acatcagaag
      181 cgctgaaaga agtactaagt gatacgggtt gggaagtttc tgaacaaacc gaatacgatg
      241 gcttacgtac tacgtcatgg acttcttatc aaactagata tgaagtttta aagcaattat
      301 gtacaaccta taaaatggca ttggattttt atatagagct tagttctaat accgtcaaag
      361 gtagatatgt ggtactcaaa aagaaaaaca gcttattcaa aggtaaagaa attgagtatg
      421 gtaaagattt ggttgggtta actaggaaga ttgatatgtc agaaatcaaa acagcattaa
      481 ttgctgtggg acccgaaaat gacaaaggaa agcgtttaga gttagttgtg actgatgacg
      541 aagcacaaag tcaattcaac ttacctaccc gttatatttg gggaatatac gaacctcaat
      601 cagatgatca aaatatgaat gaaacacggt tgcgttcttt agccaaaaca gagttaaata
      661 aacgtaagtc ggcagttatg tcatatgaga ttacttctac tgatttggaa gttacgtatc
      721 cgcacgagat tatatcaatt ggtgatacag tcagagtaaa acatagagat tttaacccgc
      781 cattgtatgt agaggcagaa gttattgccg aagaatataa cataatttca gaaaatagca
      841 catatacatt cggtcaacct aaagagttca aagaatcaga attacgagaa gagtttaaca
      901 aacgattgaa cataatacat caaaagttaa acgataatat tagcaatatc aacactatag
      961 ttaaagatgt tgtagatggt gaattagaat actttgaaca caaaatacac aaaagtgata
     1021 caccgccaga aaatccagtc aatgata
//
LOCUS       DAKSQE010000251         1040 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.256, whole genome shotgun sequence.
ACCESSION   DAKSQE010000251 DAKSQE010000000
VERSION     DAKSQE010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.256"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            154..927
                     /locus_tag="O0V33_002770"
     CDS             154..927
                     /locus_tag="O0V33_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCX8977311.1"
                     /translation="MIHSKRLKMCLCLIILSVFIGACGMKKEESSKDKQIKENFNKTL
                     SLYPTKNLEDFYDKEGFRDEEFDKGDKGTWIVDSEMVVELKDKKMESRSMVLYINRNT
                     RTTKGNFIVRELWEDSKGYAQSKDTKYPVKMEHNRIIPTKQIADDKLRKEIENFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAEYQLSNNDYNVKQLRKRYNIPTKKAPKLLIK
                     GDGDLKGSSIGHKNLEFTFVENKEENIYFTDSINFKPTE"
BASE COUNT          436 a          108 c          188 g          308 t
ORIGIN      
        1 cgctttttta aataatttgt gatgttaata tgtgaaaaat acaactaaca attaaaagcc
       61 acgaggattt aatcataaaa tttcctctag tattattaat attggaattt gaacttaact
      121 ttgcatcaaa aataaaaatg gggatgtgaa atgatgattc attcaaaaag gttgaaaatg
      181 tgtctatgct tgataatact atctgttttt ataggggctt gcggaatgaa aaaagaagaa
      241 agtagtaaag ataaacaaat taaagaaaac ttcaacaaaa cgttgagcct gtatccaact
      301 aaaaatcttg aagactttta tgataaggaa ggatttcgtg acgaagagtt tgataaagga
      361 gataaaggca cttggattgt tgattctgag atggtagttg agctgaagga taaaaaaatg
      421 gaatctagaa gtatggtgct ctatataaat cgtaatacta gaacaacgaa aggtaatttt
      481 attgttagag aattgtggga agatagtaaa gggtatgcac aaagtaaaga tacaaaatat
      541 ccagtgaaaa tggaacataa tcgaattatc ccaaccaaac agatagctga tgataaatta
      601 agaaaagaaa tcgaaaactt taaatttttt gtacaatacg gtgactttaa agatattaat
      661 gattataaag atggtgacat ttcatataat ccaaatgtac ctagttattc agcagaatat
      721 caattgagta acaatgacta caatgtgaag caacttagaa agaggtataa tataccaact
      781 aagaaagcac ctaaattatt aataaaaggt gatggtgatt taaaaggatc atctatagga
      841 cacaaaaatt tagagtttac ctttgtagaa aacaaagaag aaaacatcta ttttacggat
      901 agtattaatt tcaaacctac tgaatagggt taatagaagt ttggaatgaa aattccataa
      961 agtgttcaaa ataatttttc tttagttgta aatattaaaa actgagtcag tattaaaaat
     1021 ataaggtgga gaaatgatga
//
LOCUS       DAKSQE010000252         1028 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.257, whole genome shotgun sequence.
ACCESSION   DAKSQE010000252 DAKSQE010000000
VERSION     DAKSQE010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.257"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..>1028)
                     /locus_tag="O0V33_002771"
     CDS             complement(<1..>1028)
                     /locus_tag="O0V33_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001798254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4135 domain-containing protein"
                     /protein_id="HCX8977312.1"
                     /translation="YKNINTIIDSHYSSHAASEEIGYYLIYNPFLEKAKDELYEYLQK
                     SNEKLEIFNSKTLLKEFYEHIAQLMFNISNKAIITELHEQKSQGFLKGRNEKEELKYF
                     LTNISQKDNIIKFFNKYPVLCRKLYQVYKTQVDYMIALLQNIINDWSDIIEVFGLKNE
                     KLMSIHFGQGDTHQDGKTVAILHFGDSNKIVYKPKNLHIAKLYNTLVNFLNKENQFLD
                     LKYANILMKENYSYEEFITNSDAKSLKEVNNYYYRYGQIMGICYLLNASDIHFENIIS
                     SRDMPHVIDLETLFFNVIPRVGMSDDVQLKAQEIILNNIDNTLLIPIELQLDSRGSKV
                     DFSGLSYE"
BASE COUNT          343 a          157 c          120 g          408 t
ORIGIN      
        1 ttcataggat aaaccactaa aatcaacttt agagcctctc gaatctaact gtaactctat
       61 tggaattaaa agagtattat cgatattatt taatataatc tcttgagcct ttaattgtac
      121 atcatcagac atacctaccc ggggtataac gttaaaaaac aaagtttcta aatcaataac
      181 atgaggcata tctctagaag atattatatt ctcaaaatgt atatctgaag catttaaaag
      241 ataacaaata cccattattt gtccatacct ataatagtag ttattaacct cttttaatga
      301 cttagcatcg ctgttagtga taaattcctc ataagagtaa ttttccttca ttaatatatt
      361 cgcatatttc aaatctaaga attgattttc tttatttaaa aagttaacta gagtattgta
      421 aagttttgcg atatgtaaat ttttcggctt ataaactatc ttattgctat ctccaaaatg
      481 taaaatagcg acagtttttc catcttgatg ggtatctcct tgaccaaaat gaatactcat
      541 taatttttca tttttcaagc cgaatacttc tataatatct gaccaatcat taattatatt
      601 ttgtagcaag gcaatcatat aatctacttg tgtcttgtat acttggtata atttcctaca
      661 taaaactgga tatttgttaa aaaactttat tatattgtcc ttttgagata tgtttgtaag
      721 aaagtattta agttcttcct tttcatttct tccttttaaa aatccttgac tcttttgctc
      781 gtgtaattca gttataatag ctttatttga aatgttaaac attaactgag ctatatgttc
      841 gtaaaactcc tttagtaatg ttttactatt aaaaatttct agtttttcat ttgatttttg
      901 taagtattca tataattcat ccttagcttt ttccagaaat gggttatata ttaaataata
      961 accaatttct tcggaagcag catgactact atagtgactg tctataatag tattaatatt
     1021 cttatacc
//
LOCUS       DAKSQE010000253         1006 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.258, whole genome shotgun sequence.
ACCESSION   DAKSQE010000253 DAKSQE010000000
VERSION     DAKSQE010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.258"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..>1006
                     /locus_tag="O0V33_002772"
     rRNA            <1..>1006
                     /locus_tag="O0V33_002772"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          279 a          203 c          299 g          225 t
ORIGIN      
        1 agctttaggg ctagcctcaa gtgatgatta ttggaggtag agcactgttt ggacgagggg
       61 cccctctcgg gttaccgaat tcagacaaac tccgaatgcc aattaattta acttgggagt
      121 cagaacatgg gtgataaggt ccgtgttcga aagggaaaca gcccagacca ccagctaagg
      181 tcccaaaata tatgttaagt ggaaaaggat gtggcgttgc ccagacaact aggatgttgg
      241 cttagaagca gccatcattt aaagagtgcg taatagctca ctagtcgagt gacactgcgc
      301 cgaaaatgta ccggggctaa acatattacc gaagctgtgg attgtccttt ggacaatggt
      361 aggagagcgt tctaagggcg ttgaagcatg atcgtaagga catgtggagc gcttagaagt
      421 gagaatgccg gtgtgagtag cgaaagacgg gtgagaatcc cgtccaccga ttgactaagg
      481 tttccagagg aaggctcgtc cgctctgggt tagtcgggtc ctaagctgag gccgacaggc
      541 gtaggcgatg gataacaggt tgatattcct gtaccaccta taatcgtttt aatcgatggg
      601 gggacgcagt aggataggcg aagcgtgcga ttggattgca cgtctaagca gtaaggctga
      661 gtattaggca aatccggtac tcattaaggc tgagctgtga tggggagaag acattgtgtc
      721 ttcgagtcgt tgatttcaca ctgccgagaa aagcctctag atagaaaata ggtgcccgta
      781 ccgcaaaccg acacaggtag tcaagatgag aattctaagg tgagcgagcg aactctcgtt
      841 aaggaactcg gcaaaatgac cccgtaactt cgggagaagg ggtgctcttt agggttaacg
      901 cccagaagag ccgcagtgaa taggcccaag cgactgttta tcaaaaacac aggtctctgc
      961 taaaccgtaa ggtgatgtat aggggctgac gcctgcccgg tgctgg
//
LOCUS       DAKSQE010000254          976 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.259, whole genome shotgun sequence.
ACCESSION   DAKSQE010000254 DAKSQE010000000
VERSION     DAKSQE010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.259"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..120)
                     /locus_tag="O0V33_002773"
     CDS             complement(<1..120)
                     /locus_tag="O0V33_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977313.1"
                     /translation="MQDNKQGLQANPEYTIHYLSQEIMRLTQENAMLKAYIQEN"
     gene            complement(110..>976)
                     /locus_tag="O0V33_002774"
     CDS             complement(110..>976)
                     /locus_tag="O0V33_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977314.1"
                     /translation="GVVALTSDNNRVVLESYASSNIKSKQAPVYLYPNTDKVPGLNRF
                     AFTLSNADNAYSSDGYIMFGSDENYDYGAGIRFSKERNKGLVQIVNGRYATGGDTTIE
                     AGYGKFNMLKRRDGNRYIHIQSTDLLSVGSDDAGDRIASNSIYRRTYSAAANLHITSA
                     GTIGRSTSARKYKLSIENQYNDRDEQLEHSKAILNLPIRTWFDKAESEILARELREDR
                     KLSEDTYKLDRYVGLIAEEVENLGLKEFVTYDDKGEIEGIAYDRLWIHLIPVIKEQQL
                     RIKKLEESKNAG"
BASE COUNT          272 a          212 c          147 g          345 t
ORIGIN      
        1 attttcttgt atatacgctt ttaacatcgc gttttcttgt gttaacctca taatttcctg
       61 tgataaataa tgaattgtat attcaggatt agcttgtaat ccttgtttgt tatcctgcat
      121 tctttgactc ctccaatttc ttgattctta gttgttgttc tttgataaca gggataagat
      181 gaatccatag acgatcatac gctatacctt caatttctcc tttgtcatca tacgtgacaa
      241 actcttttaa tcctaaattc tccacctctt cagcaatcaa acctacgtat ctatcaagtt
      301 tataggtgtc ttccgataat tttctatctt ctctcagctc tctagctaaa atttcagact
      361 cagctttatc aaaccacgtt ctaataggta agttaagaat agcttttgaa tgttccagtt
      421 gttcatctct atcgttatat tgattttcga tagataactt gtatttacgc gctgatgtcg
      481 aacgcccaat tgtgccagca gaagtaatat gcaaattagc tgcggccgaa taagtacgtc
      541 tataaattga gttagaagct atcctatctc ctgcatcatc tgaacctaca gacagtaggt
      601 ctgtactctg tatatgaata tacctattac catcacgtcg tttcagcata ttaaatttgc
      661 cataccctgc ttcgattgtt gtatctccac ctgttgcata tcgtccatta acaatttgaa
      721 caagaccttt atttctttct ttagaaaacc tgatacccgc accgtaatca tagttctcat
      781 cagaaccaaa cataatataa ccgtcactcg aataagcatt atctgcatta gacagcgtga
      841 atgcaaatcg gtttaatcca ggcactttgt ctgtgtttgg atataaatac accggtgcct
      901 gtttgctttt gatattcgat gaagcgtaag actccagaac aacccgatta ttatctgacg
      961 ttagtgcaac gacacc
//
LOCUS       DAKSQE010000255          974 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.260, whole genome shotgun sequence.
ACCESSION   DAKSQE010000255 DAKSQE010000000
VERSION     DAKSQE010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.260"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..434
                     /locus_tag="O0V33_002775"
     CDS             <1..434
                     /locus_tag="O0V33_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCX8977315.1"
                     /translation="SGYFQLFKTYFVTNELVLFYKNGITLQSIVDVYINHSSDPFRQF
                     LGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIE
                     DKAIKQTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK"
     gene            448..759
                     /locus_tag="O0V33_002776"
     CDS             448..759
                     /locus_tag="O0V33_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCX8977316.1"
                     /translation="MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQST
                     GCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFIKEEQKTCKSGETITISNGEAVA
                     N"
     gene            737..>974
                     /locus_tag="O0V33_002777"
     CDS             737..>974
                     /locus_tag="O0V33_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCX8977317.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTLTSKGLSNLRVI
                     DDEANIISLITELNYIKSQAIANQGYINVRFYENS"
BASE COUNT          370 a          111 c          159 g          334 t
ORIGIN      
        1 tatcaggcta tttccaatta tttaaaactt attttgtaac taatgaatta gtgttgtttt
       61 ataaaaatgg tattacactt caatcaatag tagacgttta tattaaccat agtagtgatc
      121 catttagaca gtttctaggt aaatacttat taacttattc agaaatggga tatggtttgc
      181 ctcaaatttt agaaaaacta aaatgcttta agcctcaatt aattaagttt gtgctacaag
      241 gtgaaaagag agggaagcta gaagtagaac taaagttata ttcgcaaata ttagtaaaac
      301 aaatagaaga taaagcgata aaacagactc agtttttaca gcctatttta tttttgattt
      361 taggtttatt tattgtcgca atttatttag taattatgtt accgatgttt caaatgatgc
      421 aaagtataaa ataatggagg ttaatatatg tttaaatttc ttaagaaaac tcaagcgttt
      481 acattgatag agatgctatt agtgttatta atcatcagtt tattattaat tttaatcatt
      541 ccaaatattg ctaaacaaac tgctcacata caatcaacag gttgtaatgc acaggtaaaa
      601 atggttaata gtcaaattga agcgtatgca ttgaaacata atagaaatcc atcgtctatt
      661 gaagacttaa ttgcagatgg ttttataaaa gaagagcaaa agacatgtaa atcaggagag
      721 acaataacaa ttagtaatgg agaagcagtt gcaaattaga aaacagtcag catttactat
      781 gattgagatg cttgtggtaa tgatgttaat cagtatattt ttacttttga cattgacatc
      841 taaaggatta agcaatctta gagtaataga tgatgaggca aatatcattt ctcttattac
      901 tgaattgaat tatattaagt cgcaagctat agcaaatcaa ggatatatca atgttagatt
      961 ttatgaaaac agtg
//
LOCUS       DAKSQE010000256          960 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.261, whole genome shotgun sequence.
ACCESSION   DAKSQE010000256 DAKSQE010000000
VERSION     DAKSQE010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.261"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..744)
                     /locus_tag="O0V33_002778"
     CDS             complement(<1..744)
                     /locus_tag="O0V33_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCX8977318.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMTLNDQ
                     TITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFI
                     ALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRR
                     LRRKEYIHAQDDFVSMIKNHYPICYNTAYK"
BASE COUNT          322 a          150 c          108 g          380 t
ORIGIN      
        1 tttatatgct gtgttatagc aaatcggata gtgattttta atcatagaca caaaatcatc
       61 ttgtgcatgt atatattctt ttcttcttaa tcggcgaatt aaaaattgta catgtcttat
      121 aaaacgttgg tattgtaatg attccttatc aacagttgtc acaaggtctg attcaatgat
      181 atctatacct tttttaataa cattattgat caaggtcatc tcatgcatag ataaatcttc
      241 tgtgttagat gcaatatgta atgcaataaa tcctatctca tcttcaggaa aatgcacatc
      301 taatgctgca tttaactgat caattacctg tttagcaata tgatatgcat cactatataa
      361 ctgcatagtt tccataacaa atggattact aataacttga ttttgtttta agcgtttata
      421 agcaaatata atatgatccg ttaatgaaac tacaagttgt tttgaatcaa cattcatcgc
      481 agtattagaa ataaaattca acgaatcaat aattacttgt aatacattat catcagcgat
      541 ttcaactaaa cttttataat gtgctttttg ttgttcactc tctaatttat aaattttctc
      601 tattgtaata gtttggtcgt ttaacgtcat tccctctttt ttgttaaaac caataccttt
      661 accgattaaa ataacttctt gatcattatt agtacatact acgacattgt tgttcaatgt
      721 tttagtaaca atatattctc ccattatcat cacctatttt tctattatta agattatata
      781 tcggaaatgt ctaacttaaa agtataataa tttaaatact tgtgttctat tacacgccta
      841 tcctatatga tatatcttaa tttaatttcg atgcctctca aagtggaata actttaataa
      901 aaaaatctga ctcccatatt tgcaaataat gctatttatt tatatattat ctgaattaat
//
LOCUS       DAKSQE010000257          958 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.262, whole genome shotgun sequence.
ACCESSION   DAKSQE010000257 DAKSQE010000000
VERSION     DAKSQE010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.262"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            1..899
                     /locus_tag="O0V33_002779"
                     /pseudo
     CDS             1..899
                     /locus_tag="O0V33_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
     gene            883..>958
                     /locus_tag="O0V33_002780"
     CDS             883..>958
                     /locus_tag="O0V33_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HCX8977319.1"
                     /translation="MEILNDSVKSFEKLYLQLQDGFPVI"
BASE COUNT          391 a          110 c          148 g          309 t
ORIGIN      
        1 aaattaaaaa ttcataattt agtggtaaaa atactattgc caatatctga cttgcatata
       61 aaagaaaagc ttaatttttc aaataaatat tatattcaga aaatcaaaga ctgcttggat
      121 atattaaaga aagataaaaa aggagtagat atttgttttg aagatgcaac aaggacttct
      181 agagaaaaat tgaaagaata catggaaatt atttcaaaat atcaagttag aacagttaca
      241 tttgcggaca ctgtaggatg ttcgacacca ttagaatacg gagatatttt taattacttt
      301 gtaaaaaaat attctaacat aattttttct gctcattgtc ataacgatct agggttggct
      361 actgcaaata cattagctgc aattttaaat ggtgcgaagc aaatagaaac tacatttttg
      421 ggaattggtg agagagcggg taatgctcct attgaggaaa taattactat tttgacaaaa
      481 aaacaaatag aaagtacgga attcacttta cccgacgtat ataaaactag tattaatatt
      541 tctaaaattc tcgattttca aatatcagaa aacaaaccta taattggtga aaatatattt
      601 aaacatgaat caggaattca tcaagatggt actaaaaaaa tataaatatg tatcaatatt
      661 tagttcctag tgatttagga ttgaaaaatt cacaagttgt tcagtttcca ataagtaata
      721 tttctagtaa gaaaatcttg cacaataaat ttaaatcaat agttaacact gaagaaattg
      781 atgaaaatat ttctttttat aaacttgtga acaagttttc acctgaagta gcacctgaag
      841 atacagtgga tttacttcag ataataaaaa ggaggagtaa agatggaaat tttaaatgat
      901 tctgtaaaaa gttttgaaaa actttattta caattacaag atggttttcc agtgattg
//
LOCUS       DAKSQE010000258          955 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.263, whole genome shotgun sequence.
ACCESSION   DAKSQE010000258 DAKSQE010000000
VERSION     DAKSQE010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.263"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..>955
                     /locus_tag="O0V33_002781"
     CDS             <1..>955
                     /locus_tag="O0V33_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCX8977320.1"
                     /translation="MIKGKQHFKTIRFINTVCKSVLLVLIASVMIENTSVIKDLNKIK
                     ETEKYWNVLDDYYTIEFAPYHETKQSLIDNMVRSEQLVKASEAENNAILFKPKGDSVD
                     NDNFSPDEGNVILVNNQFWSIYHKQFQPDIPIKNQKNNVEVIIPQKFHAMRNEINQAY
                     HSWFEFVQNKNNKESKLSIQFINKNDYRIFTFDARDSRHLSFIEAPIIVNVQASDLSK
                     DFYYAMISQGGYLFKNYDALVKNIEKYHLDGEISGITNYKDSVMEMYHENNLKLTVLN
                     FSQIIIAIILIIIILFDVKYYFEQHRKLLVIKKLYGYSTLRA"
BASE COUNT          375 a          120 c          155 g          305 t
ORIGIN      
        1 atatgattaa aggtaagcag cactttaaaa caattcgttt tattaataca gtttgtaaaa
       61 gtgttctctt agtactaata gctagtgtaa tgatagaaaa tactagtgtt attaaagatt
      121 tgaataaaat aaaagaaact gagaaatatt ggaatgtatt agatgattat tacacgattg
      181 aatttgcacc ttatcacgaa acaaaacaaa gtttgattga taatatggtg cgatcagaac
      241 aattagtaaa ggctagtgaa gcagaaaata atgcgatttt attcaaacca aagggtgact
      301 ccgttgacaa tgacaacttt tcacctgatg aggggaatgt aatattagta aataatcagt
      361 tttggtcgat ttatcacaag cagtttcaac ctgatattcc aataaaaaat caaaaaaata
      421 atgtcgaagt aattattcca caaaagtttc atgcaatgcg taatgaaatc aatcaagcat
      481 atcattcatg gtttgaattt gtacaaaata aaaataataa agagagtaag ttatctatac
      541 agtttatcaa caaaaatgat tatcgaattt ttacatttga tgcacgagat agtcgccatt
      601 tgtcatttat agaggcgcca atcattgtga atgttcaggc atcagattta tcgaaggatt
      661 tttattatgc catgatcagt caaggcgggt atttattcaa aaattatgac gcgctagtaa
      721 aaaatattga aaagtatcat cttgatgggg aaatcagtgg aataaccaat tataaagata
      781 gcgtgatgga aatgtatcat gaaaacaatt tgaaattaac agtactcaac ttttcacaaa
      841 tcattatcgc aatcatttta ataattatta ttttatttga tgtgaaatat tattttgaac
      901 aacatcgaaa attactcgta atcaaaaagc tatatggtta ttcaacatta agagc
//
LOCUS       DAKSQE010000259          921 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.264, whole genome shotgun sequence.
ACCESSION   DAKSQE010000259 DAKSQE010000000
VERSION     DAKSQE010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.264"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..774
                     /locus_tag="O0V33_002782"
     CDS             <1..774
                     /locus_tag="O0V33_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCX8977321.1"
                     /translation="EGYKATNFPDAKGWVGAGTKRGLPKAMTDFQITYKCIVEQKGKG
                     AGRTAQHIYDSDGKLLASIGYENKYHDRKIGHIVVTLYNQKGDPKKIYDYQNKPIMYN
                     LDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDRRKPIDMDEKEWIDGGKFYQRPASIIA
                     IYSAKYNGYKWMEMNGLGSFNTEILPKPKGARDVIIQKGDLVKIDMQAKSVVINEEPM
                     LSEKSFGSNYFNVDSGYSELIIQPENVFDTTVKWQDRYL"
BASE COUNT          332 a          126 c          198 g          265 t
ORIGIN      
        1 gaaggatata aagcaactaa ttttcctgat gcaaaaggtt gggttggtgc tggcacgaaa
       61 cgagggctcc ctaaagcgat gacagatttt caaattacct ataaatgtat tgttgaacaa
      121 aaaggtaaag gtgccggaag aacagcacaa catatttatg atagtgatgg taagttactt
      181 gcttctattg gttatgaaaa taaatatcat gatagaaaaa taggccatat tgttgttacg
      241 ttgtataacc aaaaaggaga ccccaaaaag atatacgact atcagaataa accgataatg
      301 tataacttgg acagaatcgt tgtttatatg cggctcagaa gagtaggtaa taaattttct
      361 attaaaactt ggaaatttga tcacattaaa gacccagata gacgtaaacc tattgatatg
      421 gatgagaaag agtggataga tggcggtaag ttttatcagc gtccagcttc tatcatagct
      481 atctatagtg cgaagtataa cggttataag tggatggaga tgaatggatt aggttcattc
      541 aatacggaga ttctaccgaa accgaaaggc gcaagggatg tcattataca aaaaggtgat
      601 ttagtgaaaa tagatatgca agcaaaaagt gttgtcatca atgaggaacc aatgttgagc
      661 gagaaatcgt ttggaagtaa ttatttcaat gttgattctg ggtacagtga attaatcata
      721 caacctgaaa acgtctttga tacgacggtt aaatggcaag atagatattt atagaaagga
      781 gatgagagtg tgatacatgt tttagatttt aacgacaaga ttatagattt cctttctact
      841 gatgaccctt ccttagttag agcgattcat aaacgtaatg ttaatgacaa ttcagaaatg
      901 cttgaactgc tcatatcatc a
//
LOCUS       DAKSQE010000260          890 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.265, whole genome shotgun sequence.
ACCESSION   DAKSQE010000260 DAKSQE010000000
VERSION     DAKSQE010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.265"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..591)
                     /locus_tag="O0V33_002783"
     CDS             complement(<1..591)
                     /locus_tag="O0V33_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCX8977322.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVLIAPNKENNHLLTEASLFEKYKIISDNHPEYW"
     gene            702..>890
                     /locus_tag="O0V33_002784"
     CDS             702..>890
                     /locus_tag="O0V33_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCX8977323.1"
                     /translation="MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGM
                     CTTIEDEFNIHQFRDILSP"
BASE COUNT          316 a          113 c          137 g          324 t
ORIGIN      
        1 ccaatattct ggatggttat cacttattat tttgtatttt tcaaataaag atgcctcagt
       61 tagaagatga ttattctctt tattgggagc aatcaataca attttacctt cgcatacttt
      121 ttcagaatga acttctctta gtttaggttg atttctgcta atcccgatgt catacgtatg
      181 attattaata tctttttcaa tattttcatt tttgacatgg agagaaacat cgataaacgg
      241 atgctcgtta aagaaagatt ttaaaaattt gggcataatg aatgtcgcga tatatgaaga
      301 cacgacaaca tttaatttcg attgaaacat cgtcttttta agttggatat gtttgatgcc
      361 actttcatat tgttcaataa aactttgcgc aattggaaga aatgtatgac catcttcagt
      421 caagataatt tgatttttat aagtttcaaa tagtttcaca ttgagatgct gttctaaatt
      481 tttaatttgc ttatgtatag aaggtatagt gagattaatt tcttcactag ctaatcgata
      541 gtttaacgtc ttcgctaaaa tgacaaatgt atagtaccaa tccaaattca tgaacttacc
      601 tctttcaaaa aaagttaata attcattaga taatatttaa tttttattgc ataaaaataa
      661 tattaatatt atatcagagg ttatcgaggg ggtgaagtaa tatgattcga attcaagata
      721 atacaataag ggatggtatg caacaaagta atgttcgaaa aagtctaatt ataaaaaaag
      781 aagtattgaa acaaattaac aagttaaata taaattctgt tgaagtaggc atgtgtacaa
      841 ctatcgagga tgaatttaat attcatcaat tcagagacat tttaagtcct
//
LOCUS       DAKSQE010000261          876 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.266, whole genome shotgun sequence.
ACCESSION   DAKSQE010000261 DAKSQE010000000
VERSION     DAKSQE010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.266"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..>876
                     /locus_tag="O0V33_002785"
     CDS             <1..>876
                     /locus_tag="O0V33_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1182-like element ISSau3 family transposase"
                     /protein_id="HCX8977324.1"
                     /translation="ALYRDLVEEKIIPEIKEDGDSDLTIEEIDLIGSHLDKEIEDLNH
                     SIQNEDCTQIRKQTRKKRTEIKKFKKKFDDYSERKSKYEEQKSILKDRNSFSKTDHDA
                     TFMRMKEDHMKNGQLKPGYNLQIATNSQFVLSYDLFQNPTDTRTLIPFLTMIQNTFGY
                     LPEYIVADAGYGSEQNYMAIIDDFNKTPLITYGMFIKDKTRKFKSDIFNTQNWKYDEL
                     NDEFICPNNKRIGFKRYAYRNDRYGFKRDFKLYECDDCSACSLRQQCMKPNSKSNKKI
                     MKNYNWEYFKAQINQ"
BASE COUNT          368 a          112 c          143 g          253 t
ORIGIN      
        1 aagcattata tcgtgactta gttgaagaaa aaataatacc agagataaaa gaagatggag
       61 atagcgattt aacaatagaa gaaatagatt taattggtag tcatttagat aaagaaatcg
      121 aagatttaaa tcattctatt cagaacgaag attgtactca aattagaaaa cagacccgta
      181 aaaaaagaac tgagattaag aagttcaaaa agaaatttga tgattattcc gaaagaaaaa
      241 gtaaatatga agaacaaaaa tcgattctta aagatagaaa tagcttttct aaaactgatc
      301 atgatgcaac ttttatgaga atgaaggaag accatatgaa aaatggccaa cttaagccag
      361 gatacaattt acaaatagcg acaaattctc aatttgtttt atcctatgac ctatttcaaa
      421 acccgacaga tactagaact ttaattccat ttttaacaat gattcaaaat accttcggtt
      481 atttaccgga gtatattgta gctgatgcag gttatggtag tgagcaaaac tatatggcta
      541 ttatagatga ttttaataaa acgccactta ttacgtatgg tatgtttatt aaagataaaa
      601 cgagaaagtt taaaagtgac atttttaaca ctcaaaactg gaaatatgac gaacttaatg
      661 atgaatttat atgtcctaat aacaaaagaa taggttttaa aagatatgca taccgtaatg
      721 atagatatgg ttttaaacgt gacttcaaac tatatgaatg cgatgactgt tcagcatgtt
      781 ctttgaggca acaatgcatg aagccaaatt cgaaatccaa taagaaaatc atgaagaatt
      841 ataattggga atactttaaa gcccaaatta atcaaa
//
LOCUS       DAKSQE010000262          854 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.267, whole genome shotgun sequence.
ACCESSION   DAKSQE010000262 DAKSQE010000000
VERSION     DAKSQE010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.267"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
BASE COUNT          345 a          126 c          104 g          279 t
ORIGIN      
        1 tatatgaaga aaccacttat cacatattga cgaatggaga catcttaaac caacccggaa
       61 atttgaaatt atggacgtag gcaaggcttt tgtccaaccg tttcattgca tgaatttgga
      121 aaaacatgtc agataccttt atgaaatttc cgaatatttg catttaagta aatcataaat
      181 ttcagaagta ttatgataat atataccatt aaaaccaatt actaattcat tgttctaact
      241 atgcaggtaa ctttccgcaa taagtccaaa ataaatctaa tggataaaaa ataatcaaaa
      301 ctttaacttt aatcaatatt gaactgcaaa aatttggcac aagctaaaac aatgattaaa
      361 ccgacaggga ttgccaatga cacaggtaaa tcagtagaag aaatgatgtg tagaagttca
      421 caagtaaact ggaaaatagc aattaattaa aattcgtttc aacctttata catactaagc
      481 aaaattacac actttataaa aataatatta attttgtaaa attcagctta tttggaggat
      541 taaaagcaat gataaaacag gttctaagaa aaagtttaca tacagtaaaa gaacatatcg
      601 ataaaagtaa cttccaaaaa aacttaatat tttcacttgt catattccca ttcttatttg
      661 tgccttacat tataatagat atactcgttc atttatctaa taatcaagaa tactttaagt
      721 tattatcatc tatttcacat atatttttaa tgttctttgt aacagcaata agtttatacg
      781 ctatactttc tataccttta acatctaatt catgtaggaa atcaagcttt agaaagatta
      841 gtaaagtaat aatt
//
LOCUS       DAKSQE010000263          851 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.268, whole genome shotgun sequence.
ACCESSION   DAKSQE010000263 DAKSQE010000000
VERSION     DAKSQE010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.268"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..494)
                     /locus_tag="O0V33_002786"
     CDS             complement(<1..494)
                     /locus_tag="O0V33_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCX8977325.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LINK"
BASE COUNT          286 a          116 c          127 g          322 t
ORIGIN      
        1 tacttattaa tcaaatcata aaaaagccaa tgttcatcga aataggtatt tccgatttta
       61 attgcatttt tctcaaaacc aagaatgtcg aatcctagtg cactatagaa aactttagca
      121 cctatgttgt ttgacacaat tgatgtaagt aaagattcgt aattgttctg ccgtgcataa
      181 ttaataatga aattaatgag ctcgctatta atcgatttat cgttattagt aacaaagttg
      241 tatttaatta atgatttatg ttcttttcca acgtatcgaa tttgttctaa ggcagctgtt
      301 gcgataagtg tttcgttttc ataacagcca aatacaatac atttcggtga accttgattt
      361 aaaatgtcat gtattaaggt gtcagtcatt gtttgactat aatgtgaatc ttgagtgaat
      421 tcttcattga ctgtcgaaac aagtctctta tactcattaa aatcattatt cgttaatgtt
      481 ttaatttcaa tcattactga cctccttaat cattattaag aaagattttg tactaaaaaa
      541 atttcttaat tacatattaa aatgtttatt aaaattttgc aatcattgat ttatatgcac
      601 attcttgtaa atggattatc aatttaatta tatttatttt taagttcagg gaattatgga
      661 attggttatt ggtcgacact ggacttttat ttgtagacgg tatgcgaaga agtcaagcca
      721 gtattggaac cggtcacatt tatgatgact aagggacaat cattttcagt taaatattgc
      781 cagaaatttc aatcacgatt agaaaaaata accaaccaaa aatttttggt tggcaaattt
      841 gaattttatg t
//
LOCUS       DAKSQE010000264          839 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.269, whole genome shotgun sequence.
ACCESSION   DAKSQE010000264 DAKSQE010000000
VERSION     DAKSQE010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.269"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            58..345
                     /locus_tag="O0V33_002787"
     CDS             58..345
                     /locus_tag="O0V33_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977326.1"
                     /translation="MAKEIIDNTERFILVQIDKEGTERVVYQDFTGSFTTSEMVNHAQ
                     DFKSEENAKKIAETLNLLYQLTNKKQRVKVVKEVVERSDLSPEVTVNTETV"
     gene            403..699
                     /locus_tag="O0V33_002788"
     CDS             403..699
                     /locus_tag="O0V33_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCX8977327.1"
                     /translation="MGWFKKHEHEWRIRRLEENDKTMLSTLNEIKLGQKTQEQVNIKL
                     DKTLDAIQKEREIDEKNKKENDKNIRDMKMWVLGLVGTIFGSLIIALLRMLMGI"
BASE COUNT          329 a           96 c          167 g          247 t
ORIGIN      
        1 aggaagagta atccttagca ctatttttat acaaaaattt aaggaggtca tttaattatg
       61 gcaaaagaaa ttatcgacaa tacagaaagg tttattttag tacaaatcga caaagaaggt
      121 acagaacgtg tagtatatca agatttcaca ggaagtttta caacttctga aatggttaac
      181 catgctcaag attttaaatc tgaagaaaac gctaagaaaa ttgcggagac gttaaatttg
      241 ttatatcaat taactaacaa aaaacaacgt gtgaaagtag ttaaagaagt agttgaaaga
      301 tcagatttat ctccagaggt aacagttaac actgaaacag tatgaaaagc tatgagttag
      361 atactcataa tctttattct tttagaaagc gggtgtactg aattggggtg gttcaaaaaa
      421 cacgaacatg aatggcgcat cagaaggtta gaagagaatg ataaaacaat gctcagcaca
      481 ctcaacgaaa ttaaattagg tcaaaaaacc caagagcaag ttaacattaa attagataaa
      541 accttagatg ctattcaaaa agaaagagaa atagatgaaa agaataagaa agaaaatgat
      601 aagaacatac gtgatatgaa aatgtgggtg cttggtttag ttgggacaat atttgggtcg
      661 ctaattatag cattattgcg tatgcttatg ggcatataag agaggtgaat aaaatgttta
      721 aactaatctt tggttatagt ttctggacat gtttttggtt cggtaaatgt aaataagttt
      781 tagtcagtgc ttcggtactg actttttatt tattgttgta attatggtaa tatgcagaa
//
LOCUS       DAKSQE010000265          826 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.270, whole genome shotgun sequence.
ACCESSION   DAKSQE010000265 DAKSQE010000000
VERSION     DAKSQE010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.270"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            198..>826
                     /locus_tag="O0V33_002789"
     CDS             198..>826
                     /locus_tag="O0V33_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001795060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977328.1"
                     /translation="MTRYGSQIIQNGKEIKLRTTKEEFNATNRTLSNILNEIVQNVTD
                     GTTIRYDDNGVAQVLNVGPRGIRLNADKIDINGNREINLLIQNMRDKVDKTDIVNSLN
                     LSREGLDINVNRIGIKGGNNNRYVQIQNDSIELGGIVQRTWKGKRSTDDIFTRLKDGH
                     LRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNG"
BASE COUNT          324 a          123 c          175 g          204 t
ORIGIN      
        1 atcttaaaga gaaggaatcg caagcgtatg ctgatggtaa aatttcggaa gaagagcaac
       61 gcgctataca agatgctcaa gctaaacttg aagaggcaaa acaaaacgca gaactaaagg
      121 ctagaaacgc tgaaaagaaa gctaatgctt atacagacaa caaggtcaaa gaaagcacag
      181 atgcacagag gagaacactg actcgctatg gttctcaaat tatacaaaat ggtaaggaaa
      241 tcaaattaag aactactaaa gaagagttta atgcaaccaa tcgtacactt tcaaatatat
      301 taaacgagat tgtccaaaac gttacagatg gaacaacaat cagatatgat gataacggag
      361 tggctcaagt tttaaatgtg gggccacgtg gtattagatt aaatgctgat aaaattgata
      421 ttaacggtaa tagagaaata aaccttctta tccaaaatat gcgagataaa gtagataaaa
      481 ccgatattgt caacagcctt aatttatcaa gagagggtct tgatatcaat gttaatagaa
      541 ttggaattaa aggcggtaac aataacagat atgttcaaat acagaatgat tctattgaac
      601 taggtggtat tgtgcaacga acttggaaag gcaaacgatc aaccgatgat atattcacac
      661 gtcttaaaga tggacatcta aggtttagaa ataataccgc aggcggttca ctttatatgt
      721 cacattttgg tatttcaaca tatattgatg gagaaggcga agacggaggt tcatccggta
      781 ctattcaatg gtgggataaa acttacagtg atagcggtat gaatgg
//
LOCUS       DAKSQE010000266          826 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.271, whole genome shotgun sequence.
ACCESSION   DAKSQE010000266 DAKSQE010000000
VERSION     DAKSQE010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.271"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..629)
                     /locus_tag="O0V33_002790"
     CDS             complement(<1..629)
                     /locus_tag="O0V33_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001795060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977329.1"
                     /translation="MTRYGSQIIQNGKEIKLRTTKEEFNATNRTLSNILNEIVQNVTD
                     GTTIRYDDNGVAQALNVGPRGIRLNADKIDINGNREINLLIQNMRDKVDKTDIVNSLN
                     LSREGLDINVNRIGIKGGDNNRYVQIQNDSIELGGIVQRTWRGKRSTDDIFTRLKDGH
                     LRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNG"
BASE COUNT          209 a          181 c          121 g          315 t
ORIGIN      
        1 ccattcatgc cactatcact gtaagtttta tcccaccatt gaatcgtacc agatgaacca
       61 ccgtcttcac cttcaccatc aatataagtc gaaataccaa aatgtgacat ataaagtgaa
      121 ccgccagcgg tgttatttct aaatcttagg tgaccgtctt tcagtcgcgt aaaaatatcg
      181 tctgttgaac gtttccctct ccaagtacgt tgcacaatac cacctagttc aatagaatca
      241 ttctgtattt gaacatatct gttattgtca ccgcctttaa ttccaattct attaacattg
      301 atatcaaggc cctctcttga taaattaagg ctgttgacaa tatcggtttt atctacttta
      361 tctcgcatat tttggataag aaggtttatt tctctattac cgttaatatc aattttatca
      421 gcatttaatc taataccacg tggccccaca ttcaaagctt gagccactcc gttatcatca
      481 tatctgattg ttgttccatc tgtaacgttt tgaacaatct cgtttaatat atttgaaagt
      541 gtacgattgg ttgcattaaa ctcttcttta gtagttctta atttgatttc cttaccattt
      601 tgtataattt gagaaccata gcgagtcaat gttttcctct gtgcatctgt gctttctttg
      661 accttgttgt ctgtataaac attagctttc ttttcagcgt ttctagcctt tagttctgcg
      721 ttttgttttg cctcttcaag tttagcttga gcatcttgta tagcgcgttg ctcttcttcc
      781 gaaattttac catcagcata cgcttgcgat tccttctctt taagat
//
LOCUS       DAKSQE010000267          821 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.272, whole genome shotgun sequence.
ACCESSION   DAKSQE010000267 DAKSQE010000000
VERSION     DAKSQE010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.272"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..276
                     /locus_tag="O0V33_002791"
     CDS             <1..276
                     /locus_tag="O0V33_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCX8977330.1"
                     /translation="KLRLEIYNNNSSKGKIILFPSLKNFCFTIFYYFLFGGLSIMALS
                     MLLTLNPQNIIGFIGWLVMTAGFFLLNMSSIIDKKIYVLSKTNTVEK"
     gene            350..511
                     /locus_tag="O0V33_002792"
                     /pseudo
     CDS             350..511
                     /locus_tag="O0V33_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            512..821
                     /locus_tag="O0V33_002793"
                     /pseudo
     CDS             512..821
                     /locus_tag="O0V33_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
BASE COUNT          298 a           99 c          139 g          285 t
ORIGIN      
        1 aagctaagat tggaaattta taataataac tctagtaaag ggaaaataat tttatttcct
       61 tcattaaaaa acttttgctt tacaatattt tattattttt tatttggcgg tctttcaata
      121 atggctctaa gtatgttatt aactttaaat cctcaaaata taataggctt tattggttgg
      181 ttggtaatga ctgcaggttt ctttctgtta aacatgtcat cgattattga caaaaaaatt
      241 tatgtattat ctaaaactaa cacggtggaa aaatgatggt ttagctggat ttactgcgag
      301 ttctgtttcg tcaatacttg tatattggac caatcaaaga aatgaatttg gaataaaagg
      361 taaaaacgat tggataggac ataaactaga cgatggtatc gatagtgtta aaaaatctac
      421 agaaaaagcc gtagatagtg ttgggaatgt tgttggtgaa ggttcaaaaa gtatttctaa
      481 tcatataagc cctaagaaat ggagctggta aatgttgcta tgcgaatcta aaatcatcaa
      541 taaaaacccc aaatatagaa ttattaaata taatgatgaa tacttaatgg tcgatataat
      601 aagcacttgg attagtttat tttttccatt tatcaattgg ttcatcccaa aaagatatgt
      661 caaaatcagt aaaaaagaat ttgacgattt aaatattgtt aaacctgcta aaaaatgttt
      721 tttggccagt tgcaggtagc tctgctttgc tgggagttgc attaagaaaa tatacacatt
      781 tacttgacgt tcaacttgat aaaaaattag ttattgccat a
//
LOCUS       DAKSQE010000268          807 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.273, whole genome shotgun sequence.
ACCESSION   DAKSQE010000268 DAKSQE010000000
VERSION     DAKSQE010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.273"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            57..479
                     /locus_tag="O0V33_002794"
     CDS             57..479
                     /locus_tag="O0V33_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCX8977331.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITNFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTVTDWDIQRSFNDAVGLNLLDLQ"
     gene            666..>807
                     /locus_tag="O0V33_002795"
     CDS             666..>807
                     /locus_tag="O0V33_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HCX8977332.1"
                     /translation="MQSRYIARIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLL
                     LRL"
BASE COUNT          269 a          117 c          114 g          307 t
ORIGIN      
        1 tattttccat ttaattgtat aattaatgac atattaaaat aaaggggatt gatcatatga
       61 aattgcaaaa aagtaatcat actatactac tagtgttaga aaaaggagaa gatatcgttg
      121 aatgtataac aaactttgca gatgaccaag acttaacatt tacatctgtc agcggcattg
      181 gtgcatgtga tgacgtcgtt ttaaaatttt tcaatttaac aacgaaacaa tatgaagaaa
      241 aacatattac tgagccacta gaattgacga gcttattagg taatatttct cgtctagata
      301 atggacattt tgcacatttg catgctactt tcggtactca atcatatgaa acttttagcg
      361 gacatctcgc taaagcaatt gtttctgcaa ctgctgaaat cattcttact gtaactgatt
      421 gggacattca acgttcattc aacgatgcag tcggtttaaa tttacttgat cttcaatagc
      481 actttacttt ttagttgaat caaattttat tacaagttat cataagtgct taatcataaa
      541 tgcattctcg tttattaata tacttcaacg agttaaatta aatttttaat taggacttga
      601 atgcgtttat aactatcaca tttgaatgaa taactttttt cattagaaag tagtgtttat
      661 tattgatgca atctcgatac atcgctagaa tttacttttt aatattattt atcgtctccc
      721 tttttgagac taaaattttt caatttatga ctttaaattt gtttttagca tatatacctt
      781 ttgagcttag tctattatta cgattat
//
LOCUS       DAKSQE010000269          767 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.274, whole genome shotgun sequence.
ACCESSION   DAKSQE010000269 DAKSQE010000000
VERSION     DAKSQE010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.274"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(505..>767)
                     /locus_tag="O0V33_002796"
     CDS             complement(505..>767)
                     /locus_tag="O0V33_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCX8977333.1"
                     /translation="EICYSAITKEQADLFYQLMSLRYEWKSTIITTNIPFSSWGESFS
                     NKIVSAAIIDRLVHHSKVFKITGESYRLKDYKTEKSLNIRQS"
BASE COUNT          283 a           99 c          120 g          265 t
ORIGIN      
        1 aatatgtatt acatcatggt ttagataaaa aagaaagact taagaaaact gagaaagaac
       61 ttttgaatag ctaagtaaaa aggtcaaaca tattgttctg aatataaata agtaataacc
      121 gtacctttat tgatgcggtt attttttatg taaaaaaacg aaaaaagttt ttaaaaaagt
      181 gttggataca aaacaggttg gaaagagaag aaaaaatgac aaatatcgca aaagccaaaa
      241 gcgaaatctt ccgcaactac actttaggct ttgcaacact tgccacaata ataaaatgtt
      301 ttttatagag ggggatattc cctcccacgt attatattac gaggtgatga aaatgacaaa
      361 cttaactaaa actagaataa taatctttat taattatcta atcggaattt tagctttaat
      421 atttgccttt tatataatct taatgtcaat agcggtttaa aaatgctgtt ttatggaggt
      481 ttgaaaatgt cgttttttag tggtttaaga ttgtcgtata tttaatgatt tttcggtttt
      541 ataatctttt aatcgatatg attctccagt aattttaaat acttttgaat ggtgaactaa
      601 ccgatcaatt atagctgctg atacaatctt attactaaat gactctcccc aactcgaaaa
      661 tggaatatta gtcgtaatta ttgttgattt ccattcatac cttagtgaca ttaactggta
      721 gaataaatca gcttgttctt tggtgatggc agaataacaa atttcat
//
LOCUS       DAKSQE010000270          756 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.275, whole genome shotgun sequence.
ACCESSION   DAKSQE010000270 DAKSQE010000000
VERSION     DAKSQE010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.275"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..515
                     /locus_tag="O0V33_002797"
     CDS             <1..515
                     /locus_tag="O0V33_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HCX8977334.1"
                     /translation="IDKIFEYKKRSKDKPLSLFIDKPEDWKLYGDNQNTEIVDKLVEI
                     FWPGPLNIILKNKTSYNYMLNNSDSIAIGCVQNKTMRRFISYINSPIAITSANISGTA
                     NDILITENEAIKHMGENVRYMLRSQNKTNYKTSSTIIKVTDNKIELLREGDIKFEEIK
                     ERLGTGIIYE"
     gene            508..>756
                     /locus_tag="O0V33_002798"
     CDS             508..>756
                     /locus_tag="O0V33_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977335.1"
                     /translation="MNKLILGIYLYRIFSRAYFYLPFLLIYFLVQGYSIIQLEILMAS
                     YGIAAFLFSLYKEKCFKICNLKDSNKLVVSEIFKIIGLL"
BASE COUNT          301 a           78 c          119 g          258 t
ORIGIN      
        1 gtatcgataa aatatttgaa tataaaaagc gatcgaaaga taagccatta tcattattta
       61 ttgataagcc agaggattgg aaattgtatg gagataatca aaatacggaa atagttgata
      121 aactagttga aatattttgg cctggaccat taaatattat tttaaaaaat aaaacaagct
      181 ataattatat gctcaataat tcagatagta tagctatagg atgtgtacaa aataaaacga
      241 tgagaaggtt tatttcatat attaattcac ctatagcaat tacttcagca aatatctctg
      301 gaactgccaa tgatatttta ataactgaaa atgaagcgat taagcacatg ggtgaaaatg
      361 taagatatat gctaagaagt caaaataaaa ctaactataa aacgtctagt acgattatta
      421 aagtgacaga taataaaatt gaattattaa gagaaggaga tataaagttt gaagaaataa
      481 aagagagact aggtacaggt attatttatg aataagttaa tacttgggat ttatttatac
      541 cgaatttttt cacgagcgta cttttattta ccttttttat taatttactt tttggttcaa
      601 ggttattcca taatacaatt agaaatatta atggcgtctt atggcattgc agcattttta
      661 ttctctctat acaaagagaa gtgttttaaa atttgtaact taaaagattc taataaatta
      721 gttgttagtg aaatattcaa aatcatcggt ttattg
//
LOCUS       DAKSQE010000271          748 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.276, whole genome shotgun sequence.
ACCESSION   DAKSQE010000271 DAKSQE010000000
VERSION     DAKSQE010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.276"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..261
                     /locus_tag="O0V33_002799"
     CDS             <1..261
                     /locus_tag="O0V33_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977336.1"
                     /translation="NITDVTQNEEQLDNSDEQSQQNEKTFSQEEVSQLIKERIARERK
                     KSDERIKDAKENNDSNEVAYLLKGGKVTKVDGNQNSVSFVPG"
     gene            274..573
                     /locus_tag="O0V33_002800"
     CDS             274..573
                     /locus_tag="O0V33_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977337.1"
                     /translation="MLFDSKPNSIVMLHNYPGQSGFSEYDLFTFFKHPSIKSMTIVTN
                     KEQVKFITKSDRFQGKIVSKFCTKYFTHINIINDSYIEKLLKKLYSINMIKYKVR"
     gene            570..707
                     /locus_tag="O0V33_002801"
     CDS             570..707
                     /locus_tag="O0V33_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977338.1"
                     /translation="MILIMIDSQLDGNVTAKELSESFKELVEEFERIEKANKNSNSKD
                     K"
BASE COUNT          317 a           87 c          123 g          221 t
ORIGIN      
        1 aatattactg atgtaactca gaacgaagag caactagata acagtgatga acaatcacaa
       61 cagaatgaga aaacattttc tcaagaagaa gtatcacaat tgattaaaga gcgtatagct
      121 agagaacgca aaaaatcaga tgaacgtatt aaagatgcca aagaaaacaa tgatagcaat
      181 gaagtagctt atttattaaa aggtggtaaa gttacaaaag tagatggcaa tcaaaatagt
      241 gtatcttttg tgccagggta acaagcaacc gaattgttat ttgacagtaa accgaattca
      301 attgttatgt tacataacta tcctggccaa tcaggatttt cagaatatga tttatttaca
      361 ttttttaagc atccatcaat aaaatcaatg acaatcgtta ctaataagga acaagtaaaa
      421 tttattacca agtcagatag gtttcaaggt aaaatagtga gtaaattttg tactaaatat
      481 tttacgcata ttaatatcat taatgatagc tatattgaaa aattattaaa aaaactttat
      541 agtataaata tgattaaata taaagtgaga tgattttaat tatgattgat tcacaattag
      601 atggaaatgt aactgcaaaa gaattgagtg aatcttttaa agaattagtt gaagaatttg
      661 aacgcataga aaaagcgaat aagaattcaa attcaaaaga taaatagcgt cctttctaca
      721 cagataaaga gaaagcggtg ctgttttt
//
LOCUS       DAKSQE010000272          711 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.277, whole genome shotgun sequence.
ACCESSION   DAKSQE010000272 DAKSQE010000000
VERSION     DAKSQE010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.277"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(148..>711)
                     /locus_tag="O0V33_002802"
     CDS             complement(148..>711)
                     /locus_tag="O0V33_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCX8977339.1"
                     /translation="MGLVEKIGNSFFQSITTRTAGFNSIDIASINKSTALMLMLLMFI
                     GGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISDKYIKLSIVTINISYLF
                     ISIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYHGITKIIIIFVMLCGKV
                     GLLTLLRTFIPPKSPKNYRYTKGQIYL"
BASE COUNT          252 a           92 c          105 g          262 t
ORIGIN      
        1 gtacttatgc atttgctttt ttaaagatat aagacgtttt gtcgtcctaa aataaataaa
       61 aaacaaaaaa actacctgtt taggtagttt tttaaatgta atagattaaa acactagttc
      121 atttcttgtt aaagatggat agttatttta tagataaatt tgtcctttag tgtagcggta
      181 attttttgga ctttttggtg gtataaatgt tcttaataaa gttaatagtc ctactttacc
      241 gcaaagcata acgaatataa taattatttt agtaatacca tgatattctg tggtaaggtt
      301 catacttaac ccgactgttc caaatgcaga aaccacttcg aataataact tgattaatga
      361 tatgttcgga ttaattatcg ataatataaa agtaatgata ctgataaata gatatgagat
      421 attaatggta acaatagata gttttatata tttgtcagat atttctttat tgaatactga
      481 aacattattt tctttacgta tataatttaa tacaaaaata aacgctactg caaaagtagt
      541 tattttaatt cctccagctg cactgagagg ggcaccacca ataaacataa gtagcattaa
      601 cattaaagca gtagatttgt taatgcttgc tatatcaata ctgttaaagc ccgctgttcg
      661 tgttgttatt gattggaaaa aagaatttcc gattttttca actagtccca t
//
LOCUS       DAKSQE010000273          699 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.278, whole genome shotgun sequence.
ACCESSION   DAKSQE010000273 DAKSQE010000000
VERSION     DAKSQE010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.278"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            487..>699
                     /locus_tag="O0V33_002803"
     CDS             487..>699
                     /locus_tag="O0V33_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCX8977340.1"
                     /translation="MEYKSYNKMSNNILSILSSEKNFDNKMVVSTGSYILSELPCIVA
                     YKNNDIVGLLTYKVYDEYIEIISLDSF"
BASE COUNT          239 a           85 c          105 g          270 t
ORIGIN      
        1 tttatttcta aaataatatg ttctgaaaaa tagaagtatt ttttgaacca ctaaataacg
       61 cgctattaaa tggcttcgct atgcccgtct aacacattag tgtaaaattt tataatttga
      121 atttttgttg gagggtccct tcctagtgtg ttctctcaga caccccaacc ggcacattat
      181 tgtaagttga cttttcgata atattaaaga tttaaaatta taaagctatt aattatgatt
      241 agtggtttca aaatgttgtt ttttagaggt ttaagattgc catgaatttc gttttatttg
      301 ttctcatgtt caggtttctt tatatcaact tttattttag taaaccaaaa agagtagcaa
      361 cttcaaaatt cgtatttata gctaaaaata attttaaaat gcacatgttt aaagaatcaa
      421 aagtgtgcat ttttaaatca tcattttcaa ttaataagga ttattaacag agagaagtga
      481 cttattttgg aatataaatc ttacaacaaa atgagtaata atattttatc tatcttgtcg
      541 agtgaaaaaa atttcgataa taaaatggta gtctcaacag gtagttacat tttgtctgag
      601 ttgccatgta ttgttgccta taaaaataat gatatcgtgg ggctactaac gtataaagtt
      661 tatgatgagt atattgaaat catttcttta gatagtttt
//
LOCUS       DAKSQE010000274          684 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.279, whole genome shotgun sequence.
ACCESSION   DAKSQE010000274 DAKSQE010000000
VERSION     DAKSQE010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.279"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            334..480
                     /locus_tag="O0V33_002804"
     CDS             334..480
                     /locus_tag="O0V33_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977341.1"
                     /translation="MKLFYIAFLIIIWLNIFLGNEVIHTLTVLITTLYIVNRRKRVKN
                     DRVE"
     gene            437..664
                     /locus_tag="O0V33_002805"
     CDS             437..664
                     /locus_tag="O0V33_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977342.1"
                     /translation="MLIEERGLKMTELNEIINAIQSLFESQSGYKISKNSGVPYQTVQ
                     DLRNGKTKIEDARFRTIIKLYSYYTSLKEQS"
BASE COUNT          289 a           60 c          108 g          227 t
ORIGIN      
        1 tataaaaata caaatatctt agaattaaat caattaatta actattaaat aaaagttaac
       61 tgtatattaa ctagtataat taatcaatta tgaatagaga aaaattaatt gtcttggaat
      121 atttgtgcga attaatttta acaaagtatt gtaatttcgt ataaaaatgt aaaagggtgt
      181 agtgtaatat tataaaacat tgtaaaatat gttatacaag ggaaaatgta gacttctagt
      241 tataggcgtt ataagagaat cttaaactta ggttagcttt taatgttgaa actaagtgga
      301 aagtgataaa tataaaaatg ataggggatt ataatgaaac tattttatat cgcatttcta
      361 attattatat ggctgaatat atttttagga aatgaagtca ttcatacact gactgtttta
      421 ataacaacat tgtatattgt taatagaaga aagagggtta aaaatgacag agttgaatga
      481 aattatcaac gctattcaat ctttgtttga gtctcaatca ggctacaaaa tatcgaaaaa
      541 ttcaggggtg ccgtatcaga ccgtacaaga tttgaggaat ggtaaaacga aaatagaaga
      601 tgctagattt agaacaatta taaaactata tagttattat acatcattaa aagagcagtc
      661 ataattaaat cattaaataa agag
//
LOCUS       DAKSQE010000275          669 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.280, whole genome shotgun sequence.
ACCESSION   DAKSQE010000275 DAKSQE010000000
VERSION     DAKSQE010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.280"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            160..366
                     /locus_tag="O0V33_002806"
     CDS             160..366
                     /locus_tag="O0V33_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977343.1"
                     /translation="MNEIETIISEIEKLLTNNTPYSISKNSGVPRQTVTDLKVGNTKI
                     KDAKFKTIIKLYEYQKSSENNSEC"
BASE COUNT          274 a           76 c           90 g          229 t
ORIGIN      
        1 caagaaattt tatcaatata taataaaaaa cgccaatact attaggcatt agtttatgaa
       61 ttaactagtt tggcaactac aaagaaacaa tgtattgcta aacgtagtta ctgcattagg
      121 ataattataa ttataaatag aaagagagcg tgattaaaaa tgaatgagat tgaaactatt
      181 ataagtgaaa tagaaaagtt attaactaac aatacaccat atagtatttc aaaaaactca
      241 ggtgtaccac gtcaaacagt tactgattta aaggtaggta atactaaaat aaaggatgcc
      301 aaatttaaaa caataatcaa gttatatgag tatcaaaagt cgtcagaaaa taattcagag
      361 tgctaataaa tataccttgt ttgattagat ggacaagaga ttaaatttct attaatttca
      421 tgattgtgtg tccacacttt gttcacctca ataaaattat ctctaggttt ttaaaattgt
      481 acatgtttaa acaatcaaaa gtatgcatta ttatattatc atttccataa aaatttaaat
      541 gatctttaag tgatgatata gaaggctgaa cacagtaaat aagttaactt taatgcagta
      601 ttctatacta tacatatttt gtaataatgt ttatgcgcat aatttttcat tgataatttt
      661 ttatattag
//
LOCUS       DAKSQE010000276          663 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.281, whole genome shotgun sequence.
ACCESSION   DAKSQE010000276 DAKSQE010000000
VERSION     DAKSQE010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.281"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..>663
                     /locus_tag="O0V33_002807"
     rRNA            <1..>663
                     /locus_tag="O0V33_002807"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          162 a          145 c          211 g          145 t
ORIGIN      
        1 cgtggtggga gacagtgtca ggcgggcagt ttgactgggg cggtcgcctc ctaaaaggta
       61 acggaggcgc tcaaaggttc cctcagaatg gttggaaatc attcatagag tgtaaaggca
      121 taagggagct tgactgcgag acctacaagt cgagcagggt cgaaagacgg acttagtgat
      181 ccggtggttc cgcatggaag ggccatcgct caacggataa aagctacccc ggggataaca
      241 ggcttatctc ccccaagagt tcacatcgac ggggaggttt ggcacctcga tgtcggctca
      301 tcgcatcctg gggctgtagt cggtcccaag ggttgggctg ttcgcccatt aaagcggtac
      361 gcgagctggg ttcagaacgt cgtgagacag ttcggtccct atccgtcgtg ggcgtaggaa
      421 atttgagagg agctgtcctt agtacgagag gaccgggatg gacatacctc tggtgtacca
      481 gttgtcgtgc caacggcata gctgggtagc tatgtgtgga cgggataagt gctgaaagca
      541 tctaagcatg aagcccccct caagatgaga tttcccaact tcggttataa gatccctcaa
      601 agatgatgag gttaataggt tcgaggtgga agcatggtga catgtggagc tgacgaatac
      661 taa
//
LOCUS       DAKSQE010000277          662 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.282, whole genome shotgun sequence.
ACCESSION   DAKSQE010000277 DAKSQE010000000
VERSION     DAKSQE010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.282"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(63..662)
                     /locus_tag="O0V33_002808"
     CDS             complement(63..662)
                     /locus_tag="O0V33_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCX8977344.1"
                     /translation="MFVDDLSVTKDNYLIAVLKMLIIFLVINYGTDISYDHEYVGLFI
                     ILLGVVLLKVLNINLLSFKKLKFPHMLYIIFGFLLMYGLDYLYDTFAPPTLNEILIDE
                     ESEDAPYHIALLSTAIIPPITEEIICRGLIIRILFRNHLFLGFIVSTVFFTLIHESNT
                     LIGYLPYFYSGLIFGYTYLKTKRLEVPILIHFINNLLAM"
BASE COUNT          262 a           90 c           95 g          215 t
ORIGIN      
        1 taatactttg taaaagcttt ggtgtcatca tgtaaatatc aaaataatta atgattttat
       61 tgttacatgg ctaagaggtt attgataaaa tgtattaaaa tcggtacctc taatcgtttt
      121 gtttttaagt aagtatagcc aaaaattaaa cctgaataaa aatacggaag ataaccaatc
      181 agcgtgtttg attcatgtat tagcgtgaag aatacagttg aaactataaa acctaagaaa
      241 agatgatttc tgaataaaat tcgtataatc aatccacgac agatgatttc ttctgtaatt
      301 ggtggaatga tagcagtact aagcagagct atgtggtaag gtgcatcttc agactcttca
      361 tctatcaata tttcatttaa tgtaggcggt gcgaacgtat catataagta atctaatcca
      421 tacatcaaca aaaatccaaa tataatataa agcatatgag ggaattttaa ttttttgaaa
      481 gataataaat tgatgtttaa aactttaaga agtacaacac ctagtaatat aataaatagc
      541 ccaacatact catggtcata actgatgtct gttccataat taattactaa aaaaatgatc
      601 aacattttta aaactgcaat taaataattg tcctttgtta cagacaaatc atcaacaaaa
      661 at
//
LOCUS       DAKSQE010000278          658 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.283, whole genome shotgun sequence.
ACCESSION   DAKSQE010000278 DAKSQE010000000
VERSION     DAKSQE010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.283"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            11..310
                     /locus_tag="O0V33_002809"
     CDS             11..310
                     /locus_tag="O0V33_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HCX8977345.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     TIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTSYQKTCIQY"
     gene            228..>658
                     /locus_tag="O0V33_002810"
     CDS             228..>658
                     /locus_tag="O0V33_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCX8977346.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVI
                     SITLLIVPDLIRLNKTFLIESRELTTVDFEFFSRDILEDFKGVDRNDIEIRQQRIILH
                     KGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKS"
BASE COUNT          267 a           75 c          106 g          210 t
ORIGIN      
        1 agaattcgtt atgaaaagct ataagtgtaa aggatcattc ttaatagata gtatggctgg
       61 atttttgctt attggattga ttacattact attgatacca atgatgaatc aaatgcaagc
      121 gagtataaac cataaactac aaacaattga tgcttctaaa gtaattttga cgactgtatc
      181 taaaattaat aaagaagaac ttaagaaggg ggtaactata gggaaatatg atattaagca
      241 aagtgaccaa caaatttgtg ctatttcaaa aaataccact tcttatcaaa agacatgtat
      301 acagtattaa tgtcaaagct ttttcgctaa ttgaaatgtt agtagcgatg atggttataa
      361 gtataacttt actaattgtt ccagacttaa ttagacttaa caaaactttt ctaattgaaa
      421 gtagggaatt aacaactgta gatttcgaat ttttctcaag agatattcta gaggatttta
      481 aaggtgtaga tagaaacgat attgaaatta ggcaacagcg tatcatttta cataaaggtg
      541 aagaaatgat cgaatacaaa ttaataaata ataaaattat taaagttgta aatgacagag
      601 gaaatataac aatgattaat aatgttacag catttactgc aaatatctac tataaatc
//
LOCUS       DAKSQE010000279          658 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.284, whole genome shotgun sequence.
ACCESSION   DAKSQE010000279 DAKSQE010000000
VERSION     DAKSQE010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.284"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..373
                     /locus_tag="O0V33_002811"
     CDS             <1..373
                     /locus_tag="O0V33_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCX8977347.1"
                     /translation="HKQKKEHYIAIQEKRIDLFFKHNLNNYKNMKVTDFHKTPMGGYF
                     IVGYINDDKKYKFQASIDSGSNNQYQKNIGYHDDKLGKLFKEKDPKYKLSVDEIIEKE
                     HLDKNEYEADPPLFFFSGPIE"
BASE COUNT          281 a           71 c           96 g          210 t
ORIGIN      
        1 gcacaagcaa aaaaaagaac attatatcgc cattcaagaa aaaagaattg atttgttttt
       61 taaacataac ttaaataatt ataaaaatat gaaagtgaca gatttccata aaactccaat
      121 gggtgggtat ttcattgtag gttatataaa tgatgataaa aaatataaat tccaagcaag
      181 tatagattcg ggaagcaata atcaatatca gaaaaatatt ggttaccatg atgataaatt
      241 aggcaaatta tttaaagaaa aagaccctaa atataaactc tcagtcgatg aaataattga
      301 aaaagaacac ttggacaaaa atgaatatga agccgatcca ccgttatttt tcttttcagg
      361 tccaattgaa taaatgttat ggatagaatg gatgaaaaat ataattattt aaattttaaa
      421 gtaaacctga tcatgattta agattataag aaagaaaaat tgactaatgt taaaaccaac
      481 agtcatatga tttgcaacgt ttatacaatc atcgtaaaac tcagtatgct taatttattt
      541 aaaatgtttg tcaataagtc cagtgaaaag gagattgatt ttttgaataa aaaatctctt
      601 atgattgtaa ttataatttt aatattgatt ctaggcggaa tctttttgaa gatgaaat
//
LOCUS       DAKSQE010000280          656 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.285, whole genome shotgun sequence.
ACCESSION   DAKSQE010000280 DAKSQE010000000
VERSION     DAKSQE010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.285"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            82..656
                     /locus_tag="O0V33_002812"
                     /pseudo
     CDS             82..656
                     /locus_tag="O0V33_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181110.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
BASE COUNT          288 a           79 c           92 g          197 t
ORIGIN      
        1 aatcattaaa taaatgtaaa gctaaataaa aacgttttat tacctcttac caaactaaaa
       61 tatggaaaga ggttttttgt catgtaaaat caatatttta cagtgcaaga aaattacaaa
      121 gaaagatttt atcaaatccc taaagtattc tttaccagtg aaaattataa aaatctaact
      181 aatgatatga aaatagccta tgctatctta cgtgatcgct taaatttatc tattaaaaat
      241 aactgggttg atgaagatgg taacatttac tttgtttatt caaatgaaaa gctaatggaa
      301 atcttaaatt gtaaaaaaga aaagttaaca aaaataaaaa aagggttaga aaacgatggt
      361 ctactcattc aaaaacgacg tggtttaaat aaaccaaata ttctatattt aatgaaacct
      421 attgtcacag aacgtgatat ttacaaaata gaaaaagaag aaaacgatgt tgaactttac
      481 ggcgagaagg aagttcgaaa atcgaacgtc cagaagttcg aaaatcgaac actaatgata
      541 ctgactttaa tgacattgac tttaatgata ctgagaataa tgatatgaat gacttgaatg
      601 atataaagta caaaaatgaa attagtaatc attcaaatca atttacacat aatttt
//
LOCUS       DAKSQE010000281          650 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.286, whole genome shotgun sequence.
ACCESSION   DAKSQE010000281 DAKSQE010000000
VERSION     DAKSQE010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.286"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..>650
                     /locus_tag="O0V33_002813"
     CDS             <1..>650
                     /locus_tag="O0V33_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001800414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HCX8977348.1"
                     /translation="LNKPKLVQNFALKLAKNLDDINNKAIDRAIEDLKKFVHTDYDLI
                     NCLKKGIVYHHGSVPENVRKYIEYLYNEEPAIKYIVTTSTLLEGVNLPATSLFILEPR
                     KGRKNLNASNFKNLVGRVCRFNEIFNNSSGDLAYLLPKIHIIKGEYCSSKFNIRNFAN
                     NVIYIEKERKDIVENPLLRNNQNENKELKEKANEFLTNISDNRILKGKIAKTFIGKS"
BASE COUNT          280 a           64 c          102 g          204 t
ORIGIN      
        1 ttaaataaac cgaagctagt acaaaatttc gctttaaaat tagcgaaaaa cttagatgat
       61 atcaataata aagctattga tagagcaatt gaagatttaa agaaatttgt gcataccgac
      121 tatgatttaa taaattgttt gaaaaaagga atagtttatc atcatggatc tgtaccagaa
      181 aatgttagaa aatatataga gtacttatat aacgaagaac cagcaattaa atatatagtt
      241 actacttcaa cgttactaga aggtgttaat ctacctgcca caagtttatt tattttagag
      301 ccgagaaaag gtaggaaaaa ccttaatgct agtaatttta aaaatttagt aggtcgagtt
      361 tgtagattta atgaaatatt taacaacagt agtggtgatt tagcgtattt attgcccaaa
      421 atacacatta ttaaaggaga atattgttct tctaagttta acataagaaa ttttgcaaat
      481 aatgtaattt atattgaaaa agaaagaaaa gatatagttg aaaatccttt attaagaaat
      541 aatcaaaacg agaataaaga attaaaagaa aaagctaatg agtttttgac taatataagt
      601 gataatagaa tattgaaagg gaaaattgct aaaactttta tcggaaaatc
//
LOCUS       DAKSQE010000282          630 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.287, whole genome shotgun sequence.
ACCESSION   DAKSQE010000282 DAKSQE010000000
VERSION     DAKSQE010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.287"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..>630
                     /locus_tag="O0V33_002814"
     CDS             <1..>630
                     /locus_tag="O0V33_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HCX8977349.1"
                     /translation="VNADVDKNDLKKKSDIDSSKLFNLTSYYTDITWQLDESNKISTD
                     QLLNNTIILKDIDISILKTSSLKVEFNSSDLANQFKGKNIDIYGLYYGNKCVGLTEEK
                     TSCLYGGVTIYDGNQLDEERVIGVNVFKDGIQQEGFVIKTKKAKVTVQELDTKVRFKL
                     ENLYKIYNKDTGNIQKGCIFFHSNNHQNQSFYYDLYNIKGSVGAEFFQF"
BASE COUNT          269 a           60 c           98 g          203 t
ORIGIN      
        1 atgttaatgc tgatgtagac aaaaatgatt taaagaaaaa atctgatata gatagtagta
       61 agttatttaa tttaacaagt tattatactg atataacgtg gcaattagac gagtcaaata
      121 aaattagtac agatcaacta ctgaataata ctataatatt aaaagatatt gatatatcca
      181 tacttaaaac ttctagtttg aaagttgagt ttaactcatc agatttagca aatcaattta
      241 aagggaaaaa tatagatatt tatggactgt attatggaaa taaatgtgta ggcttaactg
      301 aagaaaaaac atcatgctta tacggaggag ttacgatata tgatggaaat caattagatg
      361 aagagagagt tataggcgtt aatgtattta aagatggtat ccaacaagaa ggttttgtta
      421 taaaaactaa aaaggctaaa gtaacagtac aagaattaga tactaaagtt cgatttaaat
      481 tagaaaattt atataaaata tacaataaag ataccggtaa catacaaaaa ggatgcattt
      541 tctttcattc taataatcat caaaatcaat cattttatta tgatttatat aacataaaag
      601 gttcagtagg agcagagttt tttcaatttt
//
LOCUS       DAKSQE010000283          603 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.288, whole genome shotgun sequence.
ACCESSION   DAKSQE010000283 DAKSQE010000000
VERSION     DAKSQE010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.288"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            88..>603
                     /locus_tag="O0V33_002815"
     CDS             88..>603
                     /locus_tag="O0V33_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSau1 family transposase"
                     /protein_id="HCX8977350.1"
                     /translation="MSYNHLTLTERARIEVLRQENYSLRSIARKLKRSVSTISREISR
                     NNLNQSYQAETAQKNYETKRKLCGRPTRFTPELGNIIKYYLKCHWSPEQIVGRLLQNQ
                     ICFKTIYRWINSNMINFELISCLRQKGKRQKPKETRGKFNIGRPISQRPKEIKKRNTF
                     GHWEADTIVSSR"
BASE COUNT          232 a          111 c           89 g          171 t
ORIGIN      
        1 cctataaaaa ttcataaaaa atgcccataa ttggcacttt gttgtaagat gttaataacc
       61 acaaaaacac atacaaggag tgccaacatg agctataacc atcttacact aactgaacgc
      121 gcacgtatag aggttttacg tcaagaaaat tattctttaa gatctattgc acgtaaatta
      181 aaacgatctg tatctactat ctcacgtgaa attagtagga ataatttaaa tcagtcatat
      241 caagctgaaa cagctcaaaa aaattatgaa accaaaagaa aactttgtgg tcgtcctact
      301 cgatttactc cagaacttgg taatatcatt aaatattatt taaaatgcca ctggtctcct
      361 gaacaaatcg taggtcgtct attacaaaac caaatctgtt ttaaaaccat ctatcgatgg
      421 attaactcta atatgattaa tttcgaactt atttcatgcc ttcgtcaaaa aggaaaaaga
      481 cagaaaccta aagaaactag aggtaagttt aatattggcc gtcctatttc acaacgtcct
      541 aaggaaataa agaaacgtaa tacattcggt cattgggaag ctgacacaat tgtatccagc
      601 aga
//
LOCUS       DAKSQE010000284          586 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.289, whole genome shotgun sequence.
ACCESSION   DAKSQE010000284 DAKSQE010000000
VERSION     DAKSQE010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.289"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(129..479)
                     /gene="scc"
                     /locus_tag="O0V33_002816"
     CDS             complement(129..479)
                     /gene="scc"
                     /locus_tag="O0V33_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-C"
                     /protein_id="HCX8977351.1"
                     /translation="MKFKKYIVAGTLAVLLSTTAVSTLDGNKADASSKKDYIIQSEFH
                     DKRIAEELKSLLDQSYVNDLAAGSLNPYYKRMIMMNQYRAKAALKSNNFAKMAEAKVG
                     LENIYKEIDEIINR"
BASE COUNT          175 a           91 c           66 g          254 t
ORIGIN      
        1 atttatattt gtctccccta ttttttgtat acattgctca tacataaatt ttaaattgca
       61 gaatttttaa ctgtacaaat aaaaaaacag gcgaagacaa ttctattttt gtctctgcct
      121 gttttttatt atctatttat aatttcatca atttctttgt aaatgttttc taatccaact
      181 ttagcttcag ccatttttgc gaaattatta ctttttagtg ctgcttttgc tctatattgg
      241 ttcatcataa tcatacgttt gtagtatggg tttaagcttc ctgcagctaa atcatttaca
      301 taagattgat caagtaatga tttcaattct tcagcaattc gtttatcatg aaactcactt
      361 tgaattatat agtctttctt actacttgca tctgctttat tcccatctaa cgttgatact
      421 gctgttgttg ataatagtac tgctaatgtt cctgctacta tatatttttt aaatttcatt
      481 gtaaattctc cttttaaaat tgatacttaa tgattatata gtcaatttta ggaaagttac
      541 attaacctgg cattaaataa catagtcgtt ttattaataa aaaata
//
LOCUS       DAKSQE010000285          583 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.290, whole genome shotgun sequence.
ACCESSION   DAKSQE010000285 DAKSQE010000000
VERSION     DAKSQE010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.290"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..565
                     /locus_tag="O0V33_002817"
     CDS             <1..565
                     /locus_tag="O0V33_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCX8977352.1"
                     /translation="YLMVNIINNWLVLFIPLLNWLTPKRYIKISQEELENLNTFKPAK
                     NNAFWPALGSSVLFSVTFRKYMPLFNVRLEKTIVIAIFFVVFLGILFFYLNLNRRLAL
                     SVFTMNKEKSQKMILLPNLKHFFFIIFAYLYFGGFSFFTLYALITIDVQNIIIFICWG
                     AVTMFFFFINIVPIGIKKAHVILLKKS"
BASE COUNT          199 a           64 c           81 g          239 t
ORIGIN      
        1 atatttgatg gtgaatataa taaataattg gttagtttta tttattccat tgcttaattg
       61 gttgacacca aaaagataca tcaagatcag tcaagaagaa ttagaaaatt taaatacttt
      121 caaacctgct aaaaacaatg cattttggcc agcattaggt agttcagttt tatttagtgt
      181 gacttttaga aagtatatgc ctttatttaa tgttagattg gaaaaaacaa tagtaatagc
      241 catatttttt gttgtatttc ttggtatttt atttttctac ctaaatttaa atcgtaggtt
      301 agcattaagt gtttttacta tgaataaaga aaaaagtcag aaaatgatat tgctacctaa
      361 tttaaaacat tttttcttta taatatttgc atatttatat ttcggtggtt tttcattttt
      421 cactttatat gccctgataa ctatagatgt tcaaaatata attatattta tctgttgggg
      481 tgcagtaact atgtttttct tttttataaa cattgttccc attggcatta aaaaagctca
      541 tgtaatactt cttaaaaaaa gctgaataat aaaggagttt ttt
//
LOCUS       DAKSQE010000286          572 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.292, whole genome shotgun sequence.
ACCESSION   DAKSQE010000286 DAKSQE010000000
VERSION     DAKSQE010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.292"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            87..476
                     /locus_tag="O0V33_002818"
     CDS             87..476
                     /locus_tag="O0V33_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HCX8977353.1"
                     /translation="MLHLHILSWVLAIILFIATYLNISKNQGGTPYFKPLHMVLRLFM
                     LLTLISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHTMFWI
                     TIALIIITMVLGVILPLGPISKLFGIG"
BASE COUNT          201 a           68 c          102 g          201 t
ORIGIN      
        1 aataatgatt aaattataag aaagtgggta tttatcccac gaagttttaa tttaataact
       61 aaaattatta aagtggggga attaatatgt tacatttaca tattttaagt tgggtattag
      121 caatcatttt atttatcgct acatacttaa atatttcaaa aaatcaaggt ggtacgccat
      181 atttcaaacc attacatatg gtgctacgat tatttatgct gttgacgtta atttcaggat
      241 tttggatatt aattcagtca tttatgaatg gcggggcaaa tcatatgttg cttacattga
      301 aaatgctgtg tggtgtagca gtagttggat tgatggaagt gtcgattgct aaaagaaaga
      361 gacatgaaca aagtcacaca atgttttgga taacaattgc attaattatc atcacaatgg
      421 tattaggtgt cattctaccg ttagggccta tatcaaaatt attcggtatt ggctaattga
      481 taagacatat agtatatgac ataaggcatg gacaattatt agtccaagac cttatgtctt
      541 ttttgattct aaaaatgttt aaacaaaaaa aa
//
LOCUS       DAKSQE010000287          553 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.293, whole genome shotgun sequence.
ACCESSION   DAKSQE010000287 DAKSQE010000000
VERSION     DAKSQE010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.293"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(1..553)
                     /locus_tag="O0V33_002819"
                     /pseudo
     CDS             complement(1..553)
                     /locus_tag="O0V33_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          185 a           89 c           79 g          200 t
ORIGIN      
        1 tcctcgaact gacatacgaa tgaaacccaa aatagctttc ataaatccaa aaacaggctc
       61 tacatcaatt tttcttattg gatttcgaat ttggcttcat gcattgatgt ctcaaagaat
      121 atgatgaaca gtcatcacat tcatatagtt tgaagtctcg tttaaaacca tatctatcat
      181 tacggtatgc atatctttta aaacctattc ttttgttatt aggacatata aattcatcat
      241 taagttcgtc atatttccaa ttttgagtgt tgaaaatgtc acttttaaac tttctagttt
      301 tatcttaaat aaacatgcca tacgtaataa gtggcgtttt attaaaatca tctataatag
      361 ccatatagtt ttgctcacta ccataacctg catcaactac aatatactct ggtaaataac
      421 gaagggattt tgaatcattg ttaaaaacgg aattaatgtt ctagtatctg ttgggttttg
      481 aaataggtca taggataaaa caaattgaga atttgtcgct atttgtaaat tgtatcctga
      541 cttaagttgg gca
//
LOCUS       DAKSQE010000288          533 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.295, whole genome shotgun sequence.
ACCESSION   DAKSQE010000288 DAKSQE010000000
VERSION     DAKSQE010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.295"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            27..>533
                     /locus_tag="O0V33_002820"
     CDS             27..>533
                     /locus_tag="O0V33_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HCX8977354.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     TNHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYNKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDRLLSTNDEIALQFNTKKHQILQHSLTIRQYEMQK
                     QQYFEKLCK"
BASE COUNT          208 a           82 c           69 g          174 t
ORIGIN      
        1 aattttcaat atcaaaggag tttatcatgt attcaaatgt tcaaccattc attaatagtg
       61 atttaactaa ttgttttcaa tcacaataca ctgaaattga atcattatta ttcaacctct
      121 tagacagccc agtaaaatat acaaaacata ttggcggtac gaatcatttt aaatattcaa
      181 cagagccaat tttagatatt ttagtaggtg tagaaaattt gcatgatatt acatccttag
      241 atgaaaaacg cttaaattat gtagggtttt atcgtttgca tcataaatat aataaaaagg
      301 ttatgatggc gaagtttaac aattttcatg aactaaaaca acaggtgcgt ctacatatca
      361 ttcaaatgaa tacccaaaca tttgaatctt atcttgcact tgatcgtctc ttatcgacta
      421 atgatgagat cgctttacaa ttcaatacta aaaagcatca aatattgcaa catagcttaa
      481 caattagaca atatgagatg caaaagcaac aatactttga aaaactatgt aaa
//
LOCUS       DAKSQE010000289          533 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.296, whole genome shotgun sequence.
ACCESSION   DAKSQE010000289 DAKSQE010000000
VERSION     DAKSQE010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.296"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..282
                     /locus_tag="O0V33_002821"
     CDS             <1..282
                     /locus_tag="O0V33_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystatin-like fold lipoprotein"
                     /protein_id="HCX8977355.1"
                     /translation="IDEVTKLEKESIQDVKNTKKYKNVERSKSYYKIYNDGEVIIMTY
                     MPFKDSNTKVSRVYKINQTSDQYEEDSNIDPEKFEKDNKPVYEENNMKK"
     gene            391..>533
                     /locus_tag="O0V33_002822"
     CDS             391..>533
                     /locus_tag="O0V33_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCX8977356.1"
                     /translation="MKKRKKRKELDYKTRLAIAKYLNDGLNIIQISKILGRDDAVLYR
                     EVN"
BASE COUNT          232 a           50 c           91 g          160 t
ORIGIN      
        1 attgatgaag ttacaaagtt agaaaaagaa agtattcaag atgtgaaaaa tacaaagaag
       61 tataaaaatg ttgagcggtc aaaaagttat tataagattt ataatgatgg tgaggttatt
      121 ataatgactt atatgccttt taaagatagt aatacaaaag tttcaagagt atataaaata
      181 aatcagacta gcgatcaata tgaagaagat tctaatattg atcctgaaaa gtttgagaaa
      241 gataataaac cagtttatga agaaaataat atgaaaaaat aaagaatcaa ctccctatac
      301 gctttagcgg tggtattggg agttgatgaa catatatcct cttataactt attttataaa
      361 taaccttttt aaaagtaaag gaggatatac atgaaaaaaa gaaaaaaacg taaagaatta
      421 gactataaaa cacgtctagc tatcgcaaag tatttaaatg atggtttaaa catcattcag
      481 atttctaaga ttttaggtcg tgatgatgcg gttctttata gagaagtaaa taa
//
LOCUS       DAKSQE010000290          524 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.297, whole genome shotgun sequence.
ACCESSION   DAKSQE010000290 DAKSQE010000000
VERSION     DAKSQE010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.297"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..>524
                     /locus_tag="O0V33_002823"
     CDS             <1..>524
                     /locus_tag="O0V33_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCX8977357.1"
                     /translation="PIETSVRIPQNDISRYVNEIFETIPDSEFDEFRHHRGATSYHPK
                     MMLKIILYAYTQSVFSGRRIEKLLHDSIRMMWLAQDQTPSYKTINRFRVNPNTDALIE
                     SLFIQFHSQCLKQNLIDDKSIFIDGTKVEASANRYTFVWKKSIQNYESKLNENSKALY
                     RDLVEEKIIPEIKE"
BASE COUNT          200 a           75 c           80 g          169 t
ORIGIN      
        1 taccaataga aacttctgtt agaattcctc aaaatgatat ttcgcgatat gttaatgaaa
       61 tttttgaaac tatacctgat agcgaattcg atgaattcag acatcatcgt ggtgcaacat
      121 cctatcatcc aaaaatgatg ttaaaaatca tcttatatgc atatactcaa tctgtatttt
      181 ctggtcgtag aatagagaaa ttacttcatg acagtattcg aatgatgtgg ttagctcaag
      241 atcaaacacc ttcttataaa actattaatc gttttagagt gaatcctaat actgatgcgc
      301 taattgaatc tttatttatt cagttccata gtcaatgttt aaagcaaaat cttattgatg
      361 ataaatcaat ttttattgat ggtacaaaag tagaagctag tgccaataga tatacatttg
      421 tgtggaagaa aagtattcaa aattacgaat cgaaattgaa cgaaaattca aaagcattat
      481 atcgtgactt agtcgaagaa aaaataatac cagagataaa agaa
//
LOCUS       DAKSQE010000291          514 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.298, whole genome shotgun sequence.
ACCESSION   DAKSQE010000291 DAKSQE010000000
VERSION     DAKSQE010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.298"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            9..134
                     /locus_tag="O0V33_002824"
     CDS             9..134
                     /locus_tag="O0V33_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977358.1"
                     /translation="MTYIPFSIITLITGIIMHMTMYFVKFECRKMPLFMTIIFTI"
     gene            89..382
                     /locus_tag="O0V33_002825"
     CDS             89..382
                     /locus_tag="O0V33_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977359.1"
                     /translation="MSENAIIHDDYFYNLKAVKTHNIAKNVNKSLLNDKGVSIGKFIQ
                     KLKGKNPTWRYPKIKWTISKNKGQSYGGSYWKLINNKGKRIASLTKEGKILRE"
BASE COUNT          208 a           53 c           90 g          163 t
ORIGIN      
        1 gtggcgatat gacatatata cccttttcta ttattacatt aattacaggt atcattatgc
       61 atatgacaat gtactttgta aaatttgaat gtcggaaaat gccattattc atgacgatta
      121 tttttacaat ctaaaagcag taaagactca taatatagcc aaaaacgtta ataaaagttt
      181 gttaaatgat aagggggtga gtattggtaa atttattcaa aagcttaaag ggaaaaatcc
      241 tacctggaga tatccaaaaa ttaaatggac tatttccaaa aataaagggc aatcatatgg
      301 tggttcttat tggaagttaa taaataacaa aggaaaaaga atagcttctt taactaaaga
      361 aggaaaaatc ttaagggaat aaggtgttgt aaattatgtg ctttgatata aataattttg
      421 atataaaaaa gttgaatttt agaaaggtaa aaaacgcaat tcatttaagt ttaagtggcg
      481 ataaatacca gtttttggag gacggtaaaa ctat
//
LOCUS       DAKSQE010000292          506 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.299, whole genome shotgun sequence.
ACCESSION   DAKSQE010000292 DAKSQE010000000
VERSION     DAKSQE010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.299"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
BASE COUNT          133 a          119 c           73 g          181 t
ORIGIN      
        1 acataaaacc taaaaacagc agtaagatga tttatgatta aaaactatct tactgctgtt
       61 cactttctat aatacttctg aatgtcttca cttatacttc tagttacaga tttagacaat
      121 cgaaaatgca cattattaaa ttatcatttc caaaactcaa tgcggctcat cgcatccatt
      181 tttttgcctg gcaacgttct actctagcgg aacgtaagtt ggctaccatc gtcgctaaga
      241 acctttcttg acttgtgaca atcgcttgct tctttcctct ccttcggctc tcgcttactc
      301 atttagctct actaaactcg ttacgctctt ttcttgtttc gtcagattca aacgttttca
      361 cttcgccaag ccatttttct ttgtgttaac ttttattttg acgttttaga cataaaaaaa
      421 aagagacctt gcggtctcaa tgcggctcat cgcatccatt tttttgcctg gcaacgttct
      481 actctagcgg aacgtaagtt cgacta
//
LOCUS       DAKSQE010000293          500 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.301, whole genome shotgun sequence.
ACCESSION   DAKSQE010000293 DAKSQE010000000
VERSION     DAKSQE010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.301"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..>500
                     /locus_tag="O0V33_002826"
     CDS             <1..>500
                     /locus_tag="O0V33_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977360.1"
                     /translation="ALFLEYRKKLQDVYTDVEDVKIAISDRFKLLQSQYTDEKYKEAL
                     EIIATKFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKTDKDGIVER
                     LDTAEAERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQE
                     AQEALK"
BASE COUNT          212 a           65 c           96 g          127 t
ORIGIN      
        1 aagctttatt tcttgagtat agaaagaaat tacaagatgt ttatacagat gtagaagatg
       61 tcaaaatcgc catttcagat agatttaaat tattacagtc acaatacact gatgaaaaat
      121 ataaagaagc gttggaaata atagcaacaa aatttggttt aacggtgaat gaagatttgc
      181 agttagtcgg agaacctaat gttgttaaat cagctattga agcagctaga gaatccacaa
      241 aagaacaatt acgtgactat gtaaaaacat cggactataa aacagacaaa gacggtattg
      301 ttgaacgttt agatactgct gaagctgaga gaacgacttt aaaaggtgaa atcaaagata
      361 aagttacgtt aaacgaatat cgaaacggat tggaagaaca aaaacaatat actgatgacc
      421 agttaagtga tttgtccaat aatcctgaga ttaaagcaag tattgaacaa gcaaatcaag
      481 aagcgcaaga agctttaaaa
//
LOCUS       DAKSQE010000294          489 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.302, whole genome shotgun sequence.
ACCESSION   DAKSQE010000294 DAKSQE010000000
VERSION     DAKSQE010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.302"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..178)
                     /locus_tag="O0V33_002827"
     CDS             complement(<1..178)
                     /locus_tag="O0V33_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977361.1"
                     /translation="MFKVNYSILSYYPDIYLHSNLAVGVAFEIIGESYHKNEIKFITQ
                     RRKILSFDDEIDNLE"
BASE COUNT          171 a           81 c           61 g          176 t
ORIGIN      
        1 gttctaaatt gtctatttca tcatcaaatg ataaaatttt tcttctttgg gttataaatt
       61 taatttcatt cttatgataa ctttctccta taatttcaaa tgccacacct acagctaaat
      121 tactatggag ataaatatct ggataataac ttaaaatcga ataattcact ttgaacataa
      181 ctaatcactc cttgtacagt ggtgaagcaa gtattattat taaattgtag gagggaaagt
      241 ccctcctgac tgattaacgc tcgaatgtcc attcaatctt tataaagtag agatttatta
      301 agattaacaa tttggtacat ttgagctttt ttattccctt cactcgcact tacttaaact
      361 tatatgctca ttatataaat ataaaaagct ataaaaattt aatggaaatg cccccttcga
      421 agtagacatt gaaaaaatga aaactttgaa gggagcattt caagacgatc cattccaatc
      481 ttttttatc
//
LOCUS       DAKSQE010000295          464 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.303, whole genome shotgun sequence.
ACCESSION   DAKSQE010000295 DAKSQE010000000
VERSION     DAKSQE010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.303"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..>464
                     /locus_tag="O0V33_002828"
     rRNA            <1..>464
                     /locus_tag="O0V33_002828"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          146 a           89 c          126 g          103 t
ORIGIN      
        1 taagggcgca cggtggatgc cttggcacta gaagccgatg aaggacgtta ctaacgacga
       61 tatgctttgg ggagctgtaa gtaagctttg atccagagat ttccgaatgg ggaaacccag
      121 catgagttat gtcatgttat cgatatgtga atacatagca tatcagaagg cacacccgga
      181 gaactgaaac atcttagtac ccggaggaag agaaagaaaa ttcgattccc ttagtagcgg
      241 cgagcgaaac gggaagagcc caaaccaaca agcttgcttg ttggggttgt aggacactct
      301 atacggagtt acaaaggacg acattagacg aatcatctgg aaagatgaat caaagaaggt
      361 aataatcctg tagtcgaaaa tgttgtctct cttgagtgga tcctgagtac gacggagcac
      421 gtgaaattcc gtcggaatct gggaggacca tctcctaagg ctaa
//
LOCUS       DAKSQE010000296          460 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.304, whole genome shotgun sequence.
ACCESSION   DAKSQE010000296 DAKSQE010000000
VERSION     DAKSQE010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.304"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..>460
                     /locus_tag="O0V33_002829"
     CDS             <1..>460
                     /locus_tag="O0V33_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HCX8977362.1"
                     /translation="YRGQANDWPMKAGLLRNDIIDDLKKEFENIYEDMAYKYPDLIEY
                     TCLNKKEYKAEDFKKRENNMAYLQHYGLRTTLIDITENPFIALLFLTSNSQVFNNATL
                     DMYNINPKIHSEQNLFSRVKMISKNKRIIAQKGAFFNFEKLLIFQNEQNVN"
BASE COUNT          201 a           56 c           70 g          133 t
ORIGIN      
        1 tatagaggtc aagcaaatga ttggccaatg aaagcaggat tattaagaaa tgatataata
       61 gatgatttaa aaaaagagtt tgagaatata tacgaagata tggcttataa gtacccagat
      121 ttaatagagt atacgtgctt aaataaaaaa gaatataaag cagaagattt taaaaaaaga
      181 gaaaacaata tggcttattt acaacattat gggctaagaa ctacattaat tgatattact
      241 gaaaacccct ttatagcgct cctattttta acttctaact cacaagtgtt taacaatgca
      301 accttagaca tgtataatat caatcctaaa atacacagcg aacaaaattt gttttcaaga
      361 gtaaaaatga ttagtaaaaa caaaagaata atagctcaaa agggtgcgtt ttttaacttc
      421 gaaaagctat tgatttttca gaatgaacaa aatgtaaata
//
LOCUS       DAKSQE010000297          459 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.305, whole genome shotgun sequence.
ACCESSION   DAKSQE010000297 DAKSQE010000000
VERSION     DAKSQE010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.305"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..101)
                     /locus_tag="O0V33_002830"
     CDS             complement(<1..101)
                     /locus_tag="O0V33_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HCX8977363.1"
                     /translation="MHAMQYHVKLPSDYNMEIIRDRVRLNGYKTDGF"
     gene            192..>459
                     /locus_tag="O0V33_002831"
     CDS             192..>459
                     /locus_tag="O0V33_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCX8977364.1"
                     /translation="MIKLNFNDLEIFITVCEEASINKAAIKLRYAQSNISQRISKLEN
                     ELGVVLLFRNQKGAKATKAGEEFLAYSKKVLRDTETIKNKMKNNT"
BASE COUNT          189 a           60 c           64 g          146 t
ORIGIN      
        1 ttaaatccat cagtcttata accatttaat cgaactctat ctctaattat ttccatattg
       61 taatcactag gcaacttaac atgatattgc attgcatgca tttagcatac ccccttatat
      121 aaaaaggata gcaataataa gtaaaatctc atattatcta attgtgatat agttatcata
      181 aaaagtgata ggtgattaaa ttgaacttta atgatttgga aatttttata actgtatgtg
      241 aagaagcatc tatcaataaa gctgcaatta aacttagata tgcacaatct aatatatctc
      301 aaagaattag caagcttgaa aatgaattag gtgtagtttt gctttttaga aatcaaaaag
      361 gtgctaaggc aactaaagca ggcgaagaat ttttagctta tagcaaaaaa gtattaagag
      421 atacagagac tataaaaaat aaaatgaaaa ataacacta
//
LOCUS       DAKSQE010000298          454 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.306, whole genome shotgun sequence.
ACCESSION   DAKSQE010000298 DAKSQE010000000
VERSION     DAKSQE010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.306"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            53..256
                     /locus_tag="O0V33_002832"
     CDS             53..256
                     /locus_tag="O0V33_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCX8977365.1"
                     /translation="MIVKLKELREKFNITQTELSIKTNISRQTINTLENGSYIPSLKT
                     AKKLSLFFNTSIESIFIFEEEDL"
BASE COUNT          183 a           59 c           61 g          151 t
ORIGIN      
        1 ttgacatcat tatatatata aattattata cagtaaatga ggagatgata tattgatagt
       61 aaaattaaag gagttgagag aaaagtttaa tattacacaa actgaactgt ctataaaaac
      121 taatatatct aggcaaacta taaacacgtt agagaatggc tcatatatac cgtcgcttaa
      181 aaccgcaaaa aaattatcat tattctttaa cactagtata gaaagcatct ttatatttga
      241 ggaggaagat ttatgaacag aaatctttta accattttat tattgatagg gtatacgtat
      301 ttaatattta actttctaac agctgaatac tccttgacgt caagaatatt ctatgcattg
      361 aactttatta gtttatcctt aatattatta gcaaaggcta aaagtaagaa attaaataaa
      421 aacacacact tccttgacga agtacaacta gttt
//
LOCUS       DAKSQE010000299          411 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.309, whole genome shotgun sequence.
ACCESSION   DAKSQE010000299 DAKSQE010000000
VERSION     DAKSQE010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.309"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..332
                     /locus_tag="O0V33_002833"
     CDS             <1..332
                     /locus_tag="O0V33_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="base excision DNA repair protein"
                     /protein_id="HCX8977366.1"
                     /translation="VLVGTTNNRKQAIITLAELHIKNGLRENVLQVISSIKGIGSWTV
                     QMFMLFQFPHVAFENIPVADIGLHRAIEKNHDLPHKSIDKSKGESYFQNMKSIDVFKY
                     WYIYMVE"
BASE COUNT          154 a           53 c           68 g          136 t
ORIGIN      
        1 aagttttagt aggtacgact aataatagaa agcaggctat cataactctt gctgaattac
       61 atataaaaaa cggcttaaga gaaaatgttt tacaagttat ttcaagtata aaaggtatag
      121 gttcttggac agtgcaaatg tttatgctat ttcaatttcc tcacgtagct tttgaaaata
      181 ttcctgtagc agacatagga ttgcacaggg ccatagaaaa aaatcatgat cttccacata
      241 aatcgattga taaaagtaag ggagaatctt acttccaaaa catgaaatct atagatgtgt
      301 ttaaatattg gtatatatac atggttgaat agatgtttaa gcagtttgtt gtacagtaaa
      361 aataaaacct attaaatata aggattgctt aatctctttt ctaattattc a
//
LOCUS       DAKSQE010000300          409 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.310, whole genome shotgun sequence.
ACCESSION   DAKSQE010000300 DAKSQE010000000
VERSION     DAKSQE010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.310"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            146..>409
                     /locus_tag="O0V33_002834"
     CDS             146..>409
                     /locus_tag="O0V33_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCX8977367.1"
                     /translation="MYKNYNMTQLTLPIETSVRIPQNDISRYVNEIVETIPDSEFDEF
                     RHHRGATSYHPKMMLKIILYAYTQSVFSGRRIEKLLHDSIRMMW"
BASE COUNT          147 a           74 c           58 g          130 t
ORIGIN      
        1 ggtcttcgac tggcactgct ccctcaggag tctcgccatt aatactacgt attaacatgt
       61 aattttactt tgaaatactt taaaaaaata agacactttc gtataattta ataaatacca
      121 ctaaactaaa ttaacgaggt gccttatgta taaaaattat aacatgaccc aacttacact
      181 accaatagaa acttctgtta gaattcctca aaatgatatt tcacgatatg ttaatgaaat
      241 tgttgaaacg atacctgata gcgaattcga tgaattcaga catcatcgtg gcgcaacatc
      301 ctatcatcca aaaatgatgt taaaaattat cttatatgca tacactcaat ctgtattttc
      361 tggtcgtaga atagagaaat tacttcatga cagtattcga atgatgtgg
//
LOCUS       DAKSQE010000301          403 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.311, whole genome shotgun sequence.
ACCESSION   DAKSQE010000301 DAKSQE010000000
VERSION     DAKSQE010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.311"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(<1..>403)
                     /locus_tag="O0V33_002835"
     rRNA            complement(<1..>403)
                     /locus_tag="O0V33_002835"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           89 a          120 c           88 g          106 t
ORIGIN      
        1 ttcacatcag acttaaaaaa ccgcctacgc gcgctttacg cccaataatt ccggataacg
       61 cttgccacct acgtattacc gcggctgctg gcacgtagtt agccgtggct ttctgattag
      121 gtaccgtcaa gatgtgcaca gttacttaca catatgttct tccctaataa cagagtttta
      181 cgatccgaag accttcatca ctcacgcggc gttgctccgt caggctttcg cccattgcgg
      241 aagattccct actgctgcct cccgtaggag tctggaccgt gtctcagttc cagtgtggcc
      301 gatcaccctc tcaggtcggc tatgcatcgt tgccttggta agccgttacc ttaccaacta
      361 gctaatgcag cgcggatcca tctataagtg acagcaagac cgt
//
LOCUS       DAKSQE010000302          394 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.313, whole genome shotgun sequence.
ACCESSION   DAKSQE010000302 DAKSQE010000000
VERSION     DAKSQE010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.313"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
BASE COUNT          138 a           60 c           78 g          118 t
ORIGIN      
        1 agctaactta ctatataatt ctttttacat aaatatcata actataaagt atcttggcat
       61 ggaaaaatgt agctactttc aaaaaaagaa acgtcgactg tagatagtgg tgaaaggaaa
      121 ttttgatttt agcaatctat taatgattgt aaaaagtaaa actattcttg ggagtcgaac
      181 ataaatcgat gtctagactc tatagtgtta tatttggcag tagttgacgg ggccccaaca
      241 taaagaaatt ggattcccaa tttctacaga caatgcaagt tgggggcggg gccccaacac
      301 aaaagctggc ggaaagtcag cttacattaa tgtgcaagtt ggggtgggcc ccaacataaa
      361 gaaatacttt ttctttagaa attagtattt ctta
//
LOCUS       DAKSQE010000303          384 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.314, whole genome shotgun sequence.
ACCESSION   DAKSQE010000303 DAKSQE010000000
VERSION     DAKSQE010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.314"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            complement(55..>384)
                     /locus_tag="O0V33_002836"
     rRNA            complement(55..>384)
                     /locus_tag="O0V33_002836"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           89 a          101 c           86 g          108 t
ORIGIN      
        1 atatgtcacg ttattccgca tcttctgaag aagatgttcc gaatatatcc ttagaaagga
       61 ggtgatccag ccgcaccttc cgatacggct accttgttac gacttcaccc caatcatttg
      121 tcccaccttc gacggctagc tcctaaaagg ttactccacc ggcttcgggt gttacaaact
      181 ctcgtggtgt gacgggcggt gtgtacaaga cccgggaacg tattcaccgt agcatgctga
      241 tctacgatta ctagcgattc cagcttcatg tagtcgagtt gcagactaca atccgaactg
      301 agaacaactt tatgggattt gcttgacctc gcggtttcgc tgccctttgt attgtccatt
      361 gtagcacgtg tgtagcccaa atca
//
LOCUS       DAKSQE010000304          366 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.317, whole genome shotgun sequence.
ACCESSION   DAKSQE010000304 DAKSQE010000000
VERSION     DAKSQE010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.317"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..>366
                     /locus_tag="O0V33_002837"
     rRNA            <1..>366
                     /locus_tag="O0V33_002837"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          103 a           77 c          109 g           77 t
ORIGIN      
        1 ttgagtgcag aagaggaaag tggaattcca tgtgtagcgg tgaaatgcgc agagatatgg
       61 aggaacacca gtggcgaagg cgactttctg gtctgtaact gacgctgatg tgcgaaagcg
      121 tggggatcaa acaggattag ataccctggt agtccacgcc gtaaacgatg agtgctaagt
      181 gttagggggt ttccgcccct tagtgctgca gctaacgcat taagcactcc gcctggggag
      241 tacgaccgca aggttgaaac tcaaaggaat tgacggggac ccgcacaagc ggtggagcat
      301 gtggtttaat tcgaagcaac gcgaagaacc ttaccaaatc ttgacatcct ttgacaactc
      361 tagaga
//
LOCUS       DAKSQE010000305          349 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.320, whole genome shotgun sequence.
ACCESSION   DAKSQE010000305 DAKSQE010000000
VERSION     DAKSQE010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.320"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            96..178
                     /locus_tag="O0V33_002838"
     tRNA            96..178
                     /locus_tag="O0V33_002838"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:129..131,aa:Leu,seq:gag)
     gene            200..273
                     /locus_tag="O0V33_002839"
     tRNA            200..273
                     /locus_tag="O0V33_002839"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:232..234,aa:Gly,seq:tcc)
BASE COUNT          121 a           62 c           69 g           97 t
ORIGIN      
        1 gtaaaaaaac gattaaaagt tattgacaaa tatttttaat cataatataa taaataacgt
       61 tctgaaaaac gaagatgttt taaatgccta tatccgcggt cgtggcggaa tggcagacgc
      121 gctaggttga gggcctagtg ggagaaatcc cgtggaggtt caagtcctct cggccgcatc
      181 aaaattctta attaaataag cgggtgtagt ttaatggcaa aacctcagcc ttccaagctg
      241 atgttgtggg ttcgattccc atcacccgct ccataaataa ttttaatgaa cattgaaaac
      301 tgaatgacaa tatgtcaacg ttaattccaa aaaacgtaac tataagtta
//
LOCUS       DAKSQE010000306          314 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.322, whole genome shotgun sequence.
ACCESSION   DAKSQE010000306 DAKSQE010000000
VERSION     DAKSQE010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.322"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
BASE COUNT          120 a           44 c           32 g          118 t
ORIGIN      
        1 tttaaacttt tatataaata caaaagtatg tttgttcaaa aatttcactt tatcgaatta
       61 aattcaatga ttttataccc tcgtacattt gaattaaaca ataataattc ttaaatcatc
      121 gaacgattaa ctttttggaa ttataactaa aaatagcgat agcatacaac ttcatataag
      181 tcatatgcta tcgccatttt cgacatcgta tttaaagtaa gatattcaat tgactgcaag
      241 taatatgtca tcagtcggag ttatttgtat taatatcaaa taattaatta cgcagacttt
      301 tctatttaaa cata
//
LOCUS       DAKSQE010000307          304 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.323, whole genome shotgun sequence.
ACCESSION   DAKSQE010000307 DAKSQE010000000
VERSION     DAKSQE010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.323"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..>304
                     /locus_tag="O0V33_002840"
     CDS             <1..>304
                     /locus_tag="O0V33_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCX8977368.1"
                     /translation="PDVAVLRRYWNGRWIEATPNDVEKLGGITREKALFSELNNIFIN
                     LSIQHASLLSEATELLNSEYLVDNDLKADLQASLDAVIDVYNQIKNNLESMTPETAT"
BASE COUNT          109 a           43 c           60 g           92 t
ORIGIN      
        1 ccctgatgtt gctgtcttgc gtagatattg gaatggtcga tggattgaag caacaccaaa
       61 tgatgttgaa aaattaggtg gtataacaag agagaaagcg ctattcagtg aattaaacaa
      121 tatttttatt aatttatcta tacaacacgc tagtcttttg tcagaagcta cagaattact
      181 gaatagcgag tacttagtag ataatgattt gaaagcggac ttacaagcaa gtttagacgc
      241 tgtgattgat gtttataatc aaattaaaaa taatttagaa tctatgacac ccgaaactgc
      301 aacg
//
LOCUS       DAKSQE010000308          289 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.denovo.326, whole genome shotgun sequence.
ACCESSION   DAKSQE010000308 DAKSQE010000000
VERSION     DAKSQE010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.denovo.326"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
BASE COUNT           92 a           61 c           37 g           99 t
ORIGIN      
        1 acataaaacc taaaaacagc agtaagatga tttatgatta aaaactatct tactgctgtt
       61 cactttttat aatacttctg aatgtcttcg cttatacttc tagtcacaga tttaaacaat
      121 caaaagtgca cattattaaa ttatcaattt cacacttcgc caagccatct ttctttgtgt
      181 taacctttat ttcaacgttt tagacataaa aaaaagagac cttgcggtct caatgcggct
      241 catcgcatcc atttttttgc ctggcaacgt tctactctag cggaacgta
//
LOCUS       DAKSQE010000309         4946 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.guided.143, whole genome shotgun sequence.
ACCESSION   DAKSQE010000309 DAKSQE010000000
VERSION     DAKSQE010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.guided.143"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            116..535
                     /gene="fosB"
                     /locus_tag="O0V33_002841"
     CDS             116..535
                     /gene="fosB"
                     /locus_tag="O0V33_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB/FosD family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HCX8977369.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFKLAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
     gene            complement(791..1726)
                     /locus_tag="O0V33_002842"
     CDS             complement(791..1726)
                     /locus_tag="O0V33_002842"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HCX8977370.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSTPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFATLAAKSIVNHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTGYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFIHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            2004..3263
                     /gene="sdpC"
                     /locus_tag="O0V33_002843"
     CDS             2004..3263
                     /gene="sdpC"
                     /locus_tag="O0V33_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HCX8977371.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSVIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKIQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYDTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGIAKNDTENTVADEPSIHEGTEKTES
                     QHHIDNQTESNHDEDHDITSKSVESAESVKHAPQSDDLTNDSNEDETEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            complement(3329..4015)
                     /locus_tag="O0V33_002844"
     CDS             complement(3329..4015)
                     /locus_tag="O0V33_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HCX8977372.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKMVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            4186..4839
                     /locus_tag="O0V33_002845"
     CDS             4186..4839
                     /locus_tag="O0V33_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HCX8977373.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPSLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
BASE COUNT         1705 a          904 c          697 g         1640 t
ORIGIN      
        1 ttattagagc ctcttatgca gttgctcagt caactgtata aaaattaatg acgtcatttc
       61 aaaaatcgat acaaaaataa tttattataa aaattctaag aaagaagtga acccgatgtt
      121 aaaatctatt aatcatatat gcttttcagt cagaaattta aacgattcaa tacattttta
      181 tagagatatt ttacttggaa aattgctatt gactggtaaa aaaactgctt attttaagct
      241 tgcaggccta tggattgctt taaatgaaga aaaagatata ccacgtaatg aaatccactt
      301 ttcatataca catatagctt tcactataga tgacagcgaa tttaaatatt ggcatcagag
      361 gttaaaagat aataacgtga atatcttaga aggaagagtt agagatatta gagatagaca
      421 atcaatttac tttactgacc ctgatggtca taagctagaa ttacatactg gcacacttga
      481 gaacagatta aattattata aagaggctaa accacatatg acattttaca aataaggcgt
      541 cattataaaa aggcctcttg aactccgtta aaattttaat taattattat ataataagag
      601 aacttttcaa acaatacagt tgttattttt actatctcaa caaacataaa taagcagtaa
      661 gatgactaca acttaagagt cttcttactg caattatctt tcaaatatat caacgttaat
      721 ataacttcta ttacgaaata ctcacattct gccctgcaat gcaaatttcg tcacatataa
      781 atatttttaa ttattttaaa aaatgatgca ctaaattagc aacgagcttc gcagttctat
      841 tgtcagcgtc atatgttgga ttcatctcag caatactaac tgaagacacc ttatcacttg
      901 gaataatacg ttttgccaat tcaagaacag tatgtggata caaacctaac actgccggcg
      961 cacttacccc aggcgcaaat gcactatcaa tgacatccat acaaatcgta aacataatga
     1021 catcatgttc atgtataaaa cgctcaatca tatctttgat tgttggtgat acgtgactca
     1081 ataattcatc tgcaaagaca taatcaatct ttttctcttt agcataatca aataaacttt
     1141 gcgtattccc accttgagca ataccaagca ctaaataacc tgtgttttca tcttcttcta
     1201 aaatttgtct aaagctcgtt ccagatgtag attgttgttc agcacgtgta tcaaaatgcg
     1261 catcaatatt tatcacacca atagattgtg ttggatagac tttacgcgtt gctaaatatt
     1321 gagcatacgc aatatcatgt ccaccaccta ataaaaacgt ttgtctatga ttaacaattg
     1381 acttcgctgc aagtgttgca aattcttttt gagtatcaat taattcctca tgatcatgat
     1441 aaacatttcc gtaatcgact aaagtttcac attgattcaa atccggcaaa cctgcaaatg
     1501 cttgtttaat cgcatctggt ccttcttttg caccaatgcg ccccttgttt aaagcaacac
     1561 ctttgtcaac agcatagcct aatataccaa cccctgatgg tgtactactt ttttccaatt
     1621 tcgacaaatc ttcaaatgtc actgtttgaa aatgcctgaa ttttttcggg tctgtttcac
     1681 tatctaacct tccagtccat aaatttggtt caccttgctt gtacacagca tttccccctc
     1741 ttatttatgt ggctttttaa caattaaagt ataacgtact acaaattttg aattcaattc
     1801 ataattaaaa agtacttatc tatatacctt ttacccctat tttctattca ctaacgaaca
     1861 cgaaagcgat ttatgttaat aaaatacgtc aagactatta cattttcatt aatattagca
     1921 cagacaattt aactcacggc ttgtaatatg tataattgtt acaaaatgat attttgcttg
     1981 ttacctaatg gaggttacat ataatgaaaa acaataaaat ttctggtttt caatgggcaa
     2041 tgacgatctt cgtctttttc gttattacaa tggcgttatc cattatgctt agagatttcc
     2101 agtctgtcat tggtgtcaaa cactttatat ttgaagttac agatctagca ccattaattg
     2161 ctgcaatcat ttgtatactc gttttcaaat ataaaaagat ccaacttgca ggtttaaaat
     2221 tctcaatcag cctgaaagta attgaacgtc tactgctagc tttaatttta cctttaatta
     2281 ttctaattat tggtatgtac agctttaata catttgcaga tagctttatt ttattacaat
     2341 caacaggctt atcagtacct attacacata ttctgattgg acatattcta atggcgttcg
     2401 tagtagaatt cggattccgt tcatacttac aaaatattgt tgaaacgaaa atgaatacgt
     2461 tctttgcaag catagttgtt ggtttaatgt actctgtatt ttcagcaaat acaacttacg
     2521 atacagaatt tgcagcttat aatttccttt acacattctc attctcaatg attctaggtg
     2581 agttaattag agctacaaaa ggacgtacga tctacatcgc aacaacattt catgcttcaa
     2641 tgacatttgg ccttattttc ttatttagcg aagaaatcgg tgatttattt tcaattaagg
     2701 tcattgctat ttcaacagca attgtagcgg ttggctatat tggtttaagc ttaattattc
     2761 gaggtattgc ttatttaaca actagacgaa accttgaaga acttgagcct aataattatt
     2821 tagaccatgt caatgacgat gaagaaacta atcatactga ggctgaaaaa tcttcttcaa
     2881 atattaaaga tgctgaaaaa acaggtgtag ctactgcatc aacggtcggt attgctaaaa
     2941 atgatactga aaatactgtg gctgacgaac caagcattca tgaaggtact gaaaaaacag
     3001 aatctcaaca tcacatagat aatcaaactg aatctaatca tgatgaagat catgacatca
     3061 cttcgaagtc agtagaatca gcagaatcag ttaaacatgc accacaaagt gacgatttaa
     3121 caaacgattc aaatgaagat gaaacagagc aatcattaaa agaacctgcg acctataaag
     3181 aagacagacg ttcatcagtt gtaatcgatg cagaaaaaca tatcgaaaaa actgaagaac
     3241 aatcttcaga taaaaacaaa taataacaaa gcgcttgcta gtacctctta aaaagatgat
     3301 gctagcaagc gcttttctat actatatatt atttttctaa aattttaacg ccctcttgag
     3361 tacctacaat aacttgatct gccatatcta agaagtagcc tgtctcaaac acacctgtca
     3421 gatgaattaa atactcatgg aacttatatg ggtcaattcc ttttggtaac ttacaatcta
     3481 aaatataatt gccattatca gttataaatg cgacatcttc gtttacacgg cgttctactt
     3541 ttatatcagc gtatgattca attttacgta atatgtgata ccagttaaat ttatccacct
     3601 ctactggtaa cttaaacgtc tcacctaagt attgaaccat tttcgtttca tcgacaacca
     3661 caacaaaacg tgatgccatt tcatctatca ctttctctct gaacagcgca ccaccaccac
     3721 ctttaattat atttaaagat ggatctactt catcagcacc atcaattgct aagtcgatat
     3781 gatcaacatc attgatttcg catattttaa tacctagttc ttttgctaaa aatgcaattt
     3841 tattagaagt gcatacacct gtaatattgt aaccacgttc tttaattagt tgcgccattt
     3901 gaggtaagag taattccatt gtacttcctg taccaattcc cagcgtcatg tcaccattga
     3961 tttgacttaa aacatcattt aatgtcatta acttgagtgc tttgacatct ttcatgaagg
     4021 tagcctccca tatttatgta atctattcaa ttcatatttt acatgactcg tataaattaa
     4081 cataccctta tttctaacat ttttttaaac atatcaatca gaatttacaa ttctaaaaat
     4141 tttttatagt acaatatact ttaacttaac gttaaggaga atgatatgat atcaattcac
     4201 gcaatttcga caggaaaaat ccaagatttg ccttatagct cgaaaagacc gatgcgctct
     4261 gctttagata aaactaagat ttcacaaaca acatggttat cttcaactgg tttcactggt
     4321 gatgaacagg cttataaaga tcatggtggg ccacataaag cagtttgtgg gtttagtaaa
     4381 cataactatg cactgtatca agatgattta ccttcactac ctactcatgc gatgtttgga
     4441 gagaatttaa catttgatta tttagacgaa tctgatgttt actttggtaa tcaatatcgt
     4501 ttaggtgaag cgttaattga ggtttctgaa attagagaac catgctggaa aattcaagca
     4561 aaatataata ttcctgattt agtgaagcgc atgtctacat ctggtaaaac aggtttctat
     4621 ttccgggtat taaaacaagg ctatgtatct ccaaatgatc agctttactt aatacaagaa
     4681 gcaccaatcg aacatcgttt atctgtacaa cagcttaatg acctttatta taatgataga
     4741 caaaatcaag atatgttacg atatgcacta aacaatccat ttctgtcacc aacaagacgc
     4801 gataaacttc aaaaaatgta taacagaaca ttgaaataat tacctttcat tataaatgtt
     4861 aaatgaactt ttcaaaacaa aaaggaatca acttacacat cgtttgtata atagtcttat
     4921 ctataactac tgatgctatt gattcc
//
LOCUS       DAKSQE010000310         4576 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.guided.150, whole genome shotgun sequence.
ACCESSION   DAKSQE010000310 DAKSQE010000000
VERSION     DAKSQE010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.guided.150"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            213..1169
                     /locus_tag="O0V33_002846"
     CDS             213..1169
                     /locus_tag="O0V33_002846"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCX8977374.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            1369..1848
                     /locus_tag="O0V33_002847"
     CDS             1369..1848
                     /locus_tag="O0V33_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCX8977375.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1863..2702
                     /gene="fakB2"
                     /locus_tag="O0V33_002848"
     CDS             1863..2702
                     /gene="fakB2"
                     /locus_tag="O0V33_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCX8977376.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTFNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSVGVAHANVIEYVDKLKKVFNEAFHVDNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2788..3321
                     /gene="msrA"
                     /locus_tag="O0V33_002849"
     CDS             2788..3321
                     /gene="msrA"
                     /locus_tag="O0V33_002849"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCX8977377.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHADNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DKHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            3314..3742
                     /gene="msrB"
                     /locus_tag="O0V33_002850"
     CDS             3314..3742
                     /gene="msrB"
                     /locus_tag="O0V33_002850"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCX8977378.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMVRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            3754..4254
                     /locus_tag="O0V33_002851"
     CDS             3754..4254
                     /locus_tag="O0V33_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCX8977379.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEYVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            4254..4475
                     /locus_tag="O0V33_002852"
     CDS             4254..4475
                     /locus_tag="O0V33_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCX8977380.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
BASE COUNT         1660 a          657 c          834 g         1425 t
ORIGIN      
        1 gttatagatt tgggtaaaca attagcaatt ggaaacatag atcacgttat gatgtggcag
       61 gtacttaatc atcattttat aaataaagta acggaattca tgatattaat atcatattcc
      121 taaaatgagt gataacaaaa tgctacataa agttaaatta tatcgaacta aatatacata
      181 ctataaataa taaaaatgag gtgttatcgc atatgttgaa ttcatttgat gcagcatatc
      241 acagtctttg tgaagaagtt ttagaaatag gaaatacacg aaatgatcgc acaaatacag
      301 gtacgatttc gaaatttggt catcaacttc gctttgactt atctaaagga tttccactat
      361 taacgacaaa gaaagtttct tttaaattag tagcgaccga attattatgg ttcattaaag
      421 gagatacaaa tatccaatac ttattaaagt ataataataa tatttggaac gaatgggctt
      481 ttgaaaatta tatcaaatca gacgagtata aaggtccaga tatgacagat ttcgggcatc
      541 gtgcattgag tgatcctgaa tttaacgaac aatataaaga acaaatgaaa caatttaagc
      601 aacgtattct tgaagatgat acatttgcga agcaattcgg tgatttagga aatgtttatg
      661 gtaaacagtg gcgagattgg gttgataaag atggtaatca ttttgatcaa cttaaaacag
      721 taattgagca aattaagcat aatccagatt caagacgaca catcgtatct gcatggaatc
      781 caacagaaat tgatacaatg gcacttccgc cttgtcatac aatgttccag ttttatgttc
      841 aagatggtaa gttaagttgc cagttatacc aacgtagcgc agatatcttt ttaggtgtgc
      901 catttaatat cgcaagctac gctttattga cgcaccttat tgccaaagaa tgtggacttg
      961 aagtaggaga atttgtgcat acatttggag atgcacatat ttattcaaat catattgatg
     1021 cgattcaaac acaattagca cgtgaaagct tcaatcctcc aacattaaaa attaacagtg
     1081 ataagtctat ttttgacata aattatgaag atttggaaat tgttgactat gaatcacatc
     1141 cagcaataaa agctccaata gcagtgtagt cgttgcatag ttagctaacc atatagacat
     1201 caaaatgaca tcaaagtatt ttcaagtgca aaaaagtact tttttgtgtt aaacgttttc
     1261 ataaattatg caaaatcatt atttctatca cactttatga taaaaattgt gttaaattaa
     1321 agataactta gtaattaaaa atgaaatgat agaagaagga ggataatcat gactttatcc
     1381 attctagttg cacatgactt gcaacgagta attggttttg aaaatcaatt accttggcac
     1441 ctaccaaatg atttgaagca tgttaaaaaa ttatcaacag gtcatacttt agtaatgggt
     1501 cgtaagacat ttgaatcgat tggtaaacca ctaccgaatc gtcgaaatgt tgtacttact
     1561 tcagatacaa gtttcaacgt agagggcgtt gatgtaattc attctattga agatatttac
     1621 caactaccgg gccatgtttt tatatttgga gggcaaacat tatttgaaga aatgattgat
     1681 aaagtggacg acatgtatat tactgttatt gaaggtaaat ttcgtggtga tacgttcttt
     1741 ccaccttata catttgaaga ctgggaagtt gcctcttcag ttgaaggtaa actagatgag
     1801 aaaaatacaa ttccacatac ctttctacat ttaattcgta aaaaataagg gggaaaacga
     1861 ccatgacaaa acagattata gtaacagact caacatccga tttatctaaa gaatatttag
     1921 aagcaaacaa catacatgta attcctttaa gtttaactat tgaaggagct tcatacgttg
     1981 accaagtaga tattacatca gaagaattta ttaatcatat tgaaaatgat gaagatgtaa
     2041 agacaagtca gccagccata ggtgaattta tatctgctta tgaagaacta ggaaaagatg
     2101 gttctgaaat cataagtatt catctttctt caggactaag tggtacattt aacactgctt
     2161 accaagcaag tcaaatggta gatgctaatg taactgttat tgattcaaaa tctatttctt
     2221 ttggtttagg gtatcaaata caacacctag tagagcttgt aaaagaaggt gtctcaactt
     2281 ctgaaatagt taaaaagtta aatcatttaa gagaaaacat taaattattt gtagttatag
     2341 ggcaattgaa tcaattaatt aaaggtggca gaattagtaa aacaaaaggt ttgattggta
     2401 atcttatgaa aattaaacca attggtacac tagatgatgg tcgcttagag cttgtgcaca
     2461 atgcgagaac tcaaaattct agtatccaat acttgaaaaa ggaaattgct gaatttatag
     2521 gagatcatga aatcaaatcc gttggtgtcg cacatgctaa cgtcattgaa tatgttgata
     2581 aattgaagaa agtttttaat gaagcttttc atgtggataa ttacgatata aatgtaacta
     2641 caccagttat ttctgcacat actggtcaag gtgcgattgg cctcgtagtc cttaagaagt
     2701 aaatttaatc ttttcagtgt taattacttc catttcaatc ctttatagac taaatttata
     2761 attagataga tagaggaggt aattcatatg acaaaagaat atgcaacatt agcaggagga
     2821 tgtttctggt gcatggttaa accatttaca tcatatccag gcatcaagtc agtcgtatct
     2881 ggttatagtg gcggtcatgc tgataaccca acttatgaac aggtatgtac gaatcaaacc
     2941 ggccatgtcg aagcagtaca aattacgttt gatccagagg ttacttcctt tgaaaatata
     3001 ttagacatat atttcaaaac atttgaccca accgatgatc aagggcaatt tttcgataga
     3061 ggcgaaagct atcaaccagt cattttctat catgataagc atcagaaaaa ggctgctgag
     3121 tttaaaaagc aacaattaaa tgaacaaggt attttcaaga aaccagtgat tacacctatt
     3181 aaaccatata aaaatttcta tccagctgaa gactaccatc aagattatta taaaaagaac
     3241 ccggtacatt attaccaata tcaacgtggt tcaggtagaa aagcgtttat agaatcacat
     3301 tgggggaatc aaaatgctta aaaaagataa aagtgaacta acagatatag aatatattgt
     3361 tacacaagaa aatggcactg aaccaccatt tatgaatgaa tattggaatc attttgctaa
     3421 agggatttat gtagataaga tttctggtaa acctttattt acatctgaag aaaagtttca
     3481 ttctgagtgc ggatggccta gcttttccaa agcgcttgat gacgatgaaa ttatagaatt
     3541 agtcgacaaa tcatttggta tggtaagaac tgaagtgcgt tcagaagaat caaatagtca
     3601 tttaggacac gtctttaatg atggacctaa agaaagtggc ggcttaagat actgtatcaa
     3661 ttccgctgca attcaattta ttccatatga aaaattagaa gaattgggtt atggcgattt
     3721 aatatcacat tttgataagt aggagtggaa agtatgttta aaaaattatt cggaaaaggc
     3781 aaagaagttc agaaagatat cgcaatttat gcaccattaa ctggagaata cgtgaaaatt
     3841 gaagatattc cagatcctgt attcgcacaa aaaatgatgg gcgaagggtt tggtattaat
     3901 ccaactgaag gagaagttgt gtctccaatt gcaggacgtg ttgacaatgt ctttccaact
     3961 aagcatgcaa ttggtttaaa agcagataac ggcttagaat tattagttca tatcggttta
     4021 gacacagttc aattagacgg tgaaggattt gaagtgttag tgtctagtgg tgatgaagtt
     4081 aacgtcggag atccattagt aagattcaac cttgaatata ttaataataa tgctaaatct
     4141 gttatttcac caattataat tactaacact gatcaagcgg cttctattaa tatttatgat
     4201 gaaaatgctg tgattaaagg tgaaactaaa gtgattgatg tgacaatgaa ctaatgaaaa
     4261 actattcgtt ttatcaattt gtcatgacag tgcgtggtcg acacgacgat aaaggtcgtc
     4321 tagcagaaga gatatttgac gatcttgctt tcccaaaaca cgatgatgat tttaacatac
     4381 tgtctgatta tattgagaca catggtgatt tcacattgcc aatgtctgta tttgatgatc
     4441 tatatgaaga atatacggaa tggctaaaat tttaatataa tttttaataa taatagttag
     4501 aaccagggtg atgcaagttg ttatcctggt tttaatttgt tgatttgtca atttaagtgc
     4561 aacagttcat cacata
//
LOCUS       DAKSQE010000311         2347 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.guided.196, whole genome shotgun sequence.
ACCESSION   DAKSQE010000311 DAKSQE010000000
VERSION     DAKSQE010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.guided.196"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            140..847
                     /gene="deoD"
                     /locus_tag="O0V33_002853"
     CDS             140..847
                     /gene="deoD"
                     /locus_tag="O0V33_002853"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCX8977381.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIVAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            854..2206
                     /gene="tet(38)"
                     /locus_tag="O0V33_002854"
     CDS             854..2206
                     /gene="tet(38)"
                     /locus_tag="O0V33_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCX8977382.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFALL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVILNRWTQSEK
                     "
BASE COUNT          752 a          347 c          427 g          821 t
ORIGIN      
        1 gcaagatcag tagagataac tgtaaaatgt tcttaatatt gtcaaaatgt aatgcttgaa
       61 agcgctttta aaaaatatta ttatatacat ggttagacaa atagacaaat tactatacaa
      121 atattgggag gaatatttta tgaaatcaac accacacatt aaaccaatga atgacgtcga
      181 aattgcagaa acggttctat tgccaggaga tccgttaaga gctaagttca ttgcagaaac
      241 atatttggac gatgtggaac agttcaatac agtgcgaaat atgtttggtt ttaccggaac
      301 atataaaggt aaaaaagttt ctgtcatggg ttcaggaatg ggtatgccat ctattggcat
      361 ttactcttat gaattaattc atacatttgg ttgtaaaaaa ttaattcgcg ttggttcttg
      421 tggcgcgatg caagaaaaca ttgatttata tgatgtgatt gttgcacaag gtgcctctac
      481 tgattcaaat tacgttcaac aatatcaatt accaggtcat tttgcgccaa ttgcttctta
      541 tcagttatta gaaaaagctg ttgaaacagc acgtgacaaa ggtgtacgtc atcatgtagg
      601 taatgtgtta tcaagtgata ttttctataa cgcggataca acagcgagtg aacgttggat
      661 gcgtatgggt attttaggtg tagaaatgga atcagctgca ttatacatga atgcaattta
      721 cgctggtgtc gaagcattag gtgtgttcac agtgagcgat catttaattc atgaaacatc
      781 aacaacacct gaggaaaggg aacgtgcatt tacagatatg attgaaattg cactgtcatt
      841 ggtgtagatg attatgaatg ttgaatattc taaaataaag aaagcagtac ctattttatt
      901 attcttattt gtattcagtt tggttataga caactcattc aaattgattt ctgtagccat
      961 tgctgatgac ttaaacattt cagtaacgac agtaagttgg caagcgacat tagccggttt
     1021 agtaattggt attggcgctg tagtatacgc ttcattatct gatgccatta gtatacgcac
     1081 actatttatt tatggcgtga tattaatcat tatcggatca attattggtt acattttcca
     1141 acatcaattc gcattacttt tagttggacg tattattcaa actgcaggtt tagctgctgc
     1201 agagacatta tatgtgatat atgttgcaaa gtatctttct aaagaggacc agaagactta
     1261 ccttggctta agtacgagca gttattcctt gtcattagtt atcggtacat tatcaggtgg
     1321 atttatttct acgtatttac actggacaaa tatgttttta attgcattaa tcgtagtatt
     1381 tacgttgcca ttcctattta aattattacc aaaagaaaat aatacaaata aagctcattt
     1441 agattttgtt ggcttaattc tagtggcaac tatcgctaca acagttatgc tgtttattac
     1501 gaactttaat tggttatata tgattggtgc cttaattgcg attatcgttt ttgcgctata
     1561 tattaaaaat gcgcaacgtc cattagtaaa caaatcattt ttccaaaata aacgttatgc
     1621 ttcattttta tttatagtat ttgtaatgta tgccatccaa ttgggttata tttttacgtt
     1681 cccattcata atggaacaaa tttatcatct tcagctagac acaacatcac tgctattagt
     1741 accgggttat attgtagcag tcatcgttgg tgcactaagt ggtaaaatcg gcgaatatct
     1801 taattcaaaa caagcgatta tcacagcaat tattttaatc gcactgagct tgattttacc
     1861 tgcatttgca gtaggtaatc acatttcaat cttcgtcatt tctatgatat tctttgcagg
     1921 tagctttgct ttaatgtatg cacctttact taacgaagcc attaaaacaa tagatcttaa
     1981 tatgacaggt gtggctattg gtttttataa tttaattatt aatgtggcgg tatctgtagg
     2041 tattgcgatt gctgcggctt taatcgattt taaagcatta aatttcccag gcaatgatgc
     2101 attaagttca catttcggta ttattttaat tattttaggt ttaatgagta ttgtcggatt
     2161 agttttattc gtcatcttaa atcgttggac acaatctgaa aaataaatag atataaattc
     2221 gcgagatata ttcgtattta tagtaaaatt aaataaagag attatataac acgaggagta
     2281 gtaagtatga aatttgagaa atatatagat cacactttat tgaagcctga gtcaacacgt
     2341 acgcaaa
//
LOCUS       DAKSQE010000312         1200 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.guided.244, whole genome shotgun sequence.
ACCESSION   DAKSQE010000312 DAKSQE010000000
VERSION     DAKSQE010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.guided.244"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            <1..795
                     /locus_tag="O0V33_002855"
     CDS             <1..795
                     /locus_tag="O0V33_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HCX8977383.1"
                     /translation="MVIQSQFLMGQSITDYNYKKPLHNDYQILDKSKIFGSNSGSFVM
                     YSMKKDKYYIYNEKESRKRYSPNSTYKIYLAMFGLDRHIINDENSRMSWNHKHYPFDA
                     WNKEQDLNTAMQNSVNWYFERISDQIPKNYTATQLKQLNYGNKNLGSYKSYWMEDSLK
                     ISNLEQVIVFKNMMEQNNHFSKKAKNQLSSSLLIKKNEKYELYGKTGTGIVNGKYNNG
                     WFVGYVITNHDKYYFATHLSDGKPSGKNAELISEKILKEMGVLNGQ"
     gene            785..1165
                     /gene="blaI"
                     /locus_tag="O0V33_002856"
     CDS             785..1165
                     /gene="blaI"
                     /locus_tag="O0V33_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCX8977384.1"
                     /translation="MANKQVEISMAEWDVMNIIWGKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
BASE COUNT          513 a          128 c          202 g          357 t
ORIGIN      
        1 atggtaattc aaagccagtt tttgatgggg caatccataa ctgattataa ttataaaaag
       61 cccttacaca acgattacca aattttagat aaaagtaaaa tttttggttc aaatagtgga
      121 tctttcgtca tgtacagcat gaagaaagac aaatactata tttataacga aaaagaaagt
      181 agaaaaaggt attctcctaa ttcaacttat aaaatttatt tagctatgtt tggacttgac
      241 cgacatatta taaatgatga aaattcacgt atgtcatgga atcataagca ttaccctttt
      301 gatgcttgga ataaggaaca agatttaaat acagcaatgc aaaattcagt taattggtac
      361 ttcgaacgta ttagcgatca aataccaaag aactatactg cgactcaact caagcaatta
      421 aattatggta ataaaaattt gggaagttat aaaagctatt ggatggaaga tagtttgaaa
      481 atatctaatc ttgaacaagt aatagttttt aaaaatatga tggaacaaaa taaccatttt
      541 agtaaaaaag caaagaatca attatcttct tcattattga ttaagaaaaa tgaaaagtat
      601 gaactgtatg ggaaaacagg tacaggtata gtaaacggga agtataataa tgggtggttt
      661 gtaggttacg taattacaaa tcatgataag tattattttg ctacacattt atcagatgga
      721 aagccatctg ggaaaaatgc tgaattaatc agtgaaaaaa tattaaaaga aatgggtgtt
      781 ttaaatggcc aataagcaag ttgaaatatc tatggctgaa tgggatgtta tgaatataat
      841 atggggtaaa aaatcagtat cagctaatga aattgtagtt gaaattcaaa aatataaaga
      901 agttagcgat aaaacgatta gaacattaat cacaagacta tataaaaaag agattataaa
      961 acgatacaaa tcagagaata tttattttta ctcatcaaat attaaagaag acgatattaa
     1021 aatgaaaact gctaaaacct ttcttaataa actgtatgga ggggacatga aaagtttagt
     1081 gctgaatttt gcgaaaaatg aagaattaaa taacaaagaa attgaagaat tgcgagacat
     1141 tttaaatgat attagtaaaa agtaattaga gatatgtttc aaacataata catatgtaaa
//
LOCUS       DAKSQE010000313         1092 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate PC0088
            SAMN25295804-rid16183773.guided.252, whole genome shotgun sequence.
ACCESSION   DAKSQE010000313 DAKSQE010000000
VERSION     DAKSQE010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25295804
            Sequence Read Archive: SRR17789858
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516351.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516351.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 16:18:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,857
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,681
            CDSs (with protein)               :: 2,681
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 66 of 111
            Pseudo Genes (incomplete)         :: 27 of 111
            Pseudo Genes (internal stop)      :: 39 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25295804-rid16183773.guided.252"
                     /isolate="PC0088"
                     /isolation_source="Swab (Sputum)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-05-08"
                     /collected_by="Hospital staff"
                     /note="genotype: ST5227"
     gene            31..876
                     /locus_tag="O0V33_002857"
     CDS             31..876
                     /locus_tag="O0V33_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaZ family penicillin-hydrolyzing class A
                     beta-lactamase PC1"
                     /protein_id="HCX8977385.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALD
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKALIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKENKKFLLDLMLNNKSGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
BASE COUNT          445 a          126 c          161 g          360 t
ORIGIN      
        1 aattacaact gtaatatcgg agggtttatt ttgaaaaagt taatattttt aattgtaatt
       61 gctttagttt taagtgcatg taattcaaac agttcacatg ccaaagagtt aaatgattta
      121 gaaaaaaaat ataatgctca tattggtgtt tatgctttag atactaaaag tggtaaggaa
      181 gtaaaattta attcagataa gagatttgcc tatgcttcaa cttcaaaagc gataaatagt
      241 gctattttgt tagaacaagt accttataat aagttaaata aaaaagtaca tattaacaaa
      301 gatgatatag ttgcttattc tcctatttta gaaaaatatg taggaaaaga tatcacttta
      361 aaagcactta ttgaggcttc aatgacatat agtgataata cagcaaacaa taaaattata
      421 aaagaaatcg gtggaatcaa aaaagttaaa caacgtctaa aagaactagg agataaagta
      481 acaaatccag ttagatatga gatagaatta aattactatt caccaaagag caaaaaagat
      541 acttcaacac ctgctgcttt cggtaagact ttaaataaac ttatcgcaaa tggaaaatta
      601 agcaaagaaa acaaaaaatt cttacttgat ttaatgttaa ataataaaag cggagatact
      661 ttaattaaag acggtgttcc aaaagactat aaggttgctg ataaaagtgg tcaagcaata
      721 acatatgctt ctagaaatga tgttgctttt gtttatccta agggccaatc tgaacctatt
      781 gttttagtca tttttacgaa taaagacaat aaaagtgata agccaaatga taagttgata
      841 agtgaaaccg ccaagagtgt aatgaaggaa ttttaatatt ctaaatgcat aataaatact
      901 gataacatct tatattttgt attatatttt gtattatcgt tgacatgtat aattttgata
      961 tcaaaaactg attttccctc tattattttc gagatttatt ttcttaatcc tctttaacaa
     1021 actagaaata ttgtatatac aaaaaattat aaataataga tgaatagttt aattataggt
     1081 gttcatcaat cg
//
